LOCUS       DAVKLX010000001       126667 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKLX010000001 DAVKLX010000000
VERSION     DAVKLX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 126667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 126667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..126667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.001"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            133..2025
                     /gene="pepO"
                     /locus_tag="VW325_000001"
     CDS             133..2025
                     /gene="pepO"
                     /locus_tag="VW325_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9733485.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEESWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2148..2777)
                     /locus_tag="VW325_000002"
     CDS             complement(2148..2777)
                     /locus_tag="VW325_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9733486.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(3117..3359)
                     /locus_tag="VW325_000003"
     CDS             complement(3117..3359)
                     /locus_tag="VW325_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733487.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            3770..5992
                     /locus_tag="VW325_000004"
     CDS             3770..5992
                     /locus_tag="VW325_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9733488.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALIYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            6022..6465
                     /locus_tag="VW325_000005"
     CDS             6022..6465
                     /locus_tag="VW325_000005"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9733489.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTDIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(6504..6919)
                     /locus_tag="VW325_000006"
                     /pseudo
     CDS             complement(6504..6919)
                     /locus_tag="VW325_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7277..7639
                     /locus_tag="VW325_000007"
     CDS             7277..7639
                     /locus_tag="VW325_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733490.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEEDVLILLSELQNFFLDVFQAKRIHRA
                     FCVRLCLKAQFLLSNNDS"
     gene            7820..8119
                     /locus_tag="VW325_000008"
     CDS             7820..8119
                     /locus_tag="VW325_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733491.1"
                     /translation="MNRDVLEFLRTETAEKISLYISEANRLEGDVTLLAPSSQDLEDI
                     KNAMLSNSNLGLKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(8214..8651)
                     /locus_tag="VW325_000009"
     CDS             complement(8214..8651)
                     /locus_tag="VW325_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733492.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLGENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(8753..9418)
                     /locus_tag="VW325_000010"
     CDS             complement(8753..9418)
                     /locus_tag="VW325_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU9733493.1"
                     /translation="MAIASGYLNSRLDKFVDWGPWTALVPFGLISVTNVEISMLSTRF
                     TGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWKASQEGRPN
                     QMSQKQVKLAAIIISIIAFLFAFVTNYIGYEGKMNLLAYVTTIAFALSLIANALNALK
                     LTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVRYIEGNSN
                     KEY"
     gene            complement(9606..10256)
                     /locus_tag="VW325_000011"
     CDS             complement(9606..10256)
                     /locus_tag="VW325_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9733494.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     NFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            10368..11210
                     /locus_tag="VW325_000012"
     CDS             10368..11210
                     /locus_tag="VW325_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9733495.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEDFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMNFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            11286..11723
                     /locus_tag="VW325_000013"
     CDS             11286..11723
                     /locus_tag="VW325_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9733496.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            11792..12094
                     /locus_tag="VW325_000014"
     CDS             11792..12094
                     /locus_tag="VW325_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733497.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLDFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(12185..13258)
                     /locus_tag="VW325_000015"
     CDS             complement(12185..13258)
                     /locus_tag="VW325_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9733498.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDAELQLYFDLFFGTILDAGISSINDLTSLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            13414..14091
                     /locus_tag="VW325_000016"
     CDS             13414..14091
                     /locus_tag="VW325_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9733499.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            14084..15058
                     /locus_tag="VW325_000017"
     CDS             14084..15058
                     /locus_tag="VW325_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9733500.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(15107..17050)
                     /gene="thrS"
                     /locus_tag="VW325_000018"
     CDS             complement(15107..17050)
                     /gene="thrS"
                     /locus_tag="VW325_000018"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9733501.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LEHYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(17108..17284)
                     /locus_tag="VW325_000019"
     CDS             complement(17108..17284)
                     /locus_tag="VW325_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9733502.1"
                     /translation="MTKQGRYYDETSYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            complement(17291..17431)
                     /locus_tag="VW325_000020"
     CDS             complement(17291..17431)
                     /locus_tag="VW325_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9733503.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            complement(17862..18062)
                     /locus_tag="VW325_000021"
     CDS             complement(17862..18062)
                     /locus_tag="VW325_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733504.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPMVILIVSLLYSYRFRFSL"
     gene            18301..18930
                     /locus_tag="VW325_000022"
     CDS             18301..18930
                     /locus_tag="VW325_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9733505.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKDQADELDLLRTKNNLNWTFVSSAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKEHISLLEK"
     gene            19071..19340
                     /gene="rpsO"
                     /locus_tag="VW325_000023"
     CDS             19071..19340
                     /gene="rpsO"
                     /locus_tag="VW325_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9733506.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            20156..20770
                     /locus_tag="VW325_000024"
     CDS             20156..20770
                     /locus_tag="VW325_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9733507.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(21062..21811)
                     /locus_tag="VW325_000025"
     CDS             complement(21062..21811)
                     /locus_tag="VW325_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9733508.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            21944..24280
                     /locus_tag="VW325_000026"
     CDS             21944..24280
                     /locus_tag="VW325_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU9733509.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNLAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            24381..25058
                     /locus_tag="VW325_000027"
     CDS             24381..25058
                     /locus_tag="VW325_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9733510.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVPHVSK"
     gene            25045..25842
                     /locus_tag="VW325_000028"
     CDS             25045..25842
                     /locus_tag="VW325_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9733511.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            25859..26962
                     /locus_tag="VW325_000029"
     CDS             25859..26962
                     /locus_tag="VW325_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9733512.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQAPANELFPGLQGFDNLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            26963..27043
                     /locus_tag="VW325_000030"
                     /pseudo
     CDS             26963..27043
                     /locus_tag="VW325_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            27263..28282
                     /gene="galE"
                     /locus_tag="VW325_000031"
     CDS             27263..28282
                     /gene="galE"
                     /locus_tag="VW325_000031"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9733513.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            28284..29249
                     /locus_tag="VW325_000032"
     CDS             28284..29249
                     /locus_tag="VW325_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9733514.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFSAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(29300..29506)
                     /locus_tag="VW325_000033"
     CDS             complement(29300..29506)
                     /locus_tag="VW325_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9733515.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            29558..30034
                     /locus_tag="VW325_000034"
     CDS             29558..30034
                     /locus_tag="VW325_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9733516.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            30043..30714
                     /locus_tag="VW325_000035"
     CDS             30043..30714
                     /locus_tag="VW325_000035"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9733517.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            30844..31182
                     /locus_tag="VW325_000036"
     CDS             30844..31182
                     /locus_tag="VW325_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9733518.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            31185..31814
                     /locus_tag="VW325_000037"
     CDS             31185..31814
                     /locus_tag="VW325_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9733519.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            32054..33205
                     /locus_tag="VW325_000038"
     CDS             32054..33205
                     /locus_tag="VW325_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9733520.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            33518..34267
                     /gene="truA"
                     /locus_tag="VW325_000039"
     CDS             33518..34267
                     /gene="truA"
                     /locus_tag="VW325_000039"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9733521.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            34257..35030
                     /locus_tag="VW325_000040"
     CDS             34257..35030
                     /locus_tag="VW325_000040"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9733522.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            35008..35469
                     /locus_tag="VW325_000041"
     CDS             35008..35469
                     /locus_tag="VW325_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9733523.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            35696..37037
                     /locus_tag="VW325_000042"
                     /pseudo
     CDS             35696..37037
                     /locus_tag="VW325_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(37165..37368)
                     /locus_tag="VW325_000043"
     CDS             complement(37165..37368)
                     /locus_tag="VW325_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9733524.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(37361..38443)
                     /locus_tag="VW325_000044"
     CDS             complement(37361..38443)
                     /locus_tag="VW325_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9733525.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(38569..39351)
                     /locus_tag="VW325_000045"
     CDS             complement(38569..39351)
                     /locus_tag="VW325_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9733526.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(39351..40694)
                     /locus_tag="VW325_000046"
     CDS             complement(39351..40694)
                     /locus_tag="VW325_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9733527.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(40793..42109)
                     /locus_tag="VW325_000047"
     CDS             complement(40793..42109)
                     /locus_tag="VW325_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9733528.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            42302..43528
                     /locus_tag="VW325_000048"
     CDS             42302..43528
                     /locus_tag="VW325_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9733529.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            43727..45301
                     /locus_tag="VW325_000049"
     CDS             43727..45301
                     /locus_tag="VW325_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9733530.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRIEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            45566..46354
                     /gene="codY"
                     /locus_tag="VW325_000050"
     CDS             45566..46354
                     /gene="codY"
                     /locus_tag="VW325_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9733531.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            46354..46929
                     /locus_tag="VW325_000051"
     CDS             46354..46929
                     /locus_tag="VW325_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9733532.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            47011..47096
                     /locus_tag="VW325_000052"
     tRNA            47011..47096
                     /locus_tag="VW325_000052"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47045..47047,aa:Leu,seq:aag)
     gene            47379..48509
                     /gene="ugpC"
                     /locus_tag="VW325_000053"
     CDS             47379..48509
                     /gene="ugpC"
                     /locus_tag="VW325_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9733533.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            49020..49778
                     /locus_tag="VW325_000054"
     CDS             49020..49778
                     /locus_tag="VW325_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9733534.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            49865..50377
                     /locus_tag="VW325_000055"
     CDS             49865..50377
                     /locus_tag="VW325_000055"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9733535.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            50559..51503
                     /locus_tag="VW325_000056"
     CDS             50559..51503
                     /locus_tag="VW325_000056"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9733536.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYRYL"
     gene            51512..52189
                     /locus_tag="VW325_000057"
     CDS             51512..52189
                     /locus_tag="VW325_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9733537.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEISPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            52287..53045
                     /locus_tag="VW325_000058"
     CDS             52287..53045
                     /locus_tag="VW325_000058"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9733538.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            53083..54588
                     /gene="lytC"
                     /locus_tag="VW325_000059"
     CDS             53083..54588
                     /gene="lytC"
                     /locus_tag="VW325_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU9733539.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWYGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            55084..55602
                     /locus_tag="VW325_000060"
     CDS             55084..55602
                     /locus_tag="VW325_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9733540.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            55732..56238
                     /locus_tag="VW325_000061"
     CDS             55732..56238
                     /locus_tag="VW325_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9733541.1"
                     /translation="MTKKIVAIWAQDKEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPGELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            56238..56408
                     /locus_tag="VW325_000062"
     CDS             56238..56408
                     /locus_tag="VW325_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733542.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            56440..57672
                     /gene="clpX"
                     /locus_tag="VW325_000063"
     CDS             56440..57672
                     /gene="clpX"
                     /locus_tag="VW325_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9733543.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            57681..58268
                     /locus_tag="VW325_000064"
     CDS             57681..58268
                     /locus_tag="VW325_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9733544.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            58279..58659
                     /locus_tag="VW325_000065"
     CDS             58279..58659
                     /locus_tag="VW325_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9733545.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            58711..59601
                     /gene="rapZ"
                     /locus_tag="VW325_000066"
     CDS             58711..59601
                     /gene="rapZ"
                     /locus_tag="VW325_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9733546.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKCLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            59598..60575
                     /locus_tag="VW325_000067"
     CDS             59598..60575
                     /locus_tag="VW325_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9733547.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            60572..61483
                     /gene="whiA"
                     /locus_tag="VW325_000068"
     CDS             60572..61483
                     /gene="whiA"
                     /locus_tag="VW325_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9733548.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHYQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(61517..62482)
                     /locus_tag="VW325_000069"
     CDS             complement(61517..62482)
                     /locus_tag="VW325_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9733549.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            62618..63475
                     /locus_tag="VW325_000070"
     CDS             62618..63475
                     /locus_tag="VW325_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9733550.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            63462..64241
                     /locus_tag="VW325_000071"
     CDS             63462..64241
                     /locus_tag="VW325_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU9733551.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            64265..65617
                     /locus_tag="VW325_000072"
     CDS             64265..65617
                     /locus_tag="VW325_000072"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9733552.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            65691..66065
                     /locus_tag="VW325_000073"
     CDS             65691..66065
                     /locus_tag="VW325_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9733553.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            66067..66915
                     /locus_tag="VW325_000074"
     CDS             66067..66915
                     /locus_tag="VW325_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9733554.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNDPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            67181..67948
                     /locus_tag="VW325_000075"
     CDS             67181..67948
                     /locus_tag="VW325_000075"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9733555.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLPDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            67960..69144
                     /locus_tag="VW325_000076"
     CDS             67960..69144
                     /locus_tag="VW325_000076"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9733556.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLENISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQNKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQARGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            69141..71012
                     /locus_tag="VW325_000077"
     CDS             69141..71012
                     /locus_tag="VW325_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9733557.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDATLLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLEPIAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(71155..72339)
                     /gene="mntE"
                     /locus_tag="VW325_000078"
     CDS             complement(71155..72339)
                     /gene="mntE"
                     /locus_tag="VW325_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9733558.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDILRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            72643..75339
                     /locus_tag="VW325_000079"
     CDS             72643..75339
                     /locus_tag="VW325_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9733559.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALITFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            75352..76143
                     /gene="yghU"
                     /locus_tag="VW325_000080"
     CDS             75352..76143
                     /gene="yghU"
                     /locus_tag="VW325_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU9733560.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            76259..76669
                     /locus_tag="VW325_000081"
     CDS             76259..76669
                     /locus_tag="VW325_000081"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9733561.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETTRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            76681..78276
                     /locus_tag="VW325_000082"
     CDS             76681..78276
                     /locus_tag="VW325_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733562.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            78443..78607
                     /locus_tag="VW325_000083"
     CDS             78443..78607
                     /locus_tag="VW325_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9733563.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            78686..79414
                     /gene="yaaA"
                     /locus_tag="VW325_000084"
     CDS             78686..79414
                     /gene="yaaA"
                     /locus_tag="VW325_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9733564.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(79465..80949)
                     /locus_tag="VW325_000085"
     CDS             complement(79465..80949)
                     /locus_tag="VW325_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9733565.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            81165..87926
                     /locus_tag="VW325_000086"
     CDS             81165..87926
                     /locus_tag="VW325_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9733566.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAASVLLGTALVLGVAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTEEV
                     TKPAEKVEEAKDKKEEVTHQDAVDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADATEAELSAAESSIQSFIIGLELRSNSNKETVSETPVAKKADAVESKEGAK
                     PAATTERSAVDSAILPTSTADKVETTSAPASINEILKLGLSLSDARQNPAIRKENVNR
                     GYSGFRAASNPANPIVSGSGNTVAFADISQGGRSYSFRGYGNSRGGNSIHYDVTTVRS
                     GNSVNFTISYSAPGDSREFVNNNFILDKGDGFGNPSNATITSSNPRVREQSKSISQGA
                     NYVSHSGYSMTSAISTNTEQTIRFSLPIINLNGDLSVRLKPVTFNVDQGGGGAATSND
                     PYSNSNYYYRANPLYLDANPYGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEK
                     GQRQITYKVHRFGNETLLGLPISNSVTKEAKPRIMQIGVAKDLIDTVKPRVDQNKVGD
                     TNNLTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDQGERGLTGAKGEKGDRGER
                     GLTGAQGAKGEKGDRGERGLTGAQGAKGEKGAQGERGLTGAKGEKGDQGERGLTGAQG
                     EKGDRGERGLTGAKGEKGAQGQAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTSTV
                     VRDGFDGASPLVATQRNDADKTTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEKG
                     DKGEQGLQGRDGEQGPKGEDGKTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDG
                     ASPLVSTHRNEADKTTTVIFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGK
                     DGFTPEVTVTDNNNGTHTITITQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTL
                     TFYIDKDGDGSYTAGKDELVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGD
                     TFVNTQTGDVFVKKGNTWEPAGNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGEK
                     GDQGERGLTGSKGEKGDQGERGLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPAG
                     PQGPKGPKGEQGNPGTPGKDGKSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITD
                     GRNGTNGQSPTITATRSVQNGKNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTIT
                     EGQNGTHTLTVHNPGSPDVTTTIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYIN
                     TETGDVYVKNNGNWDKEGNIKGPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVG
                     RDGRDGKDVLNGKANPEAHQGKDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGE
                     RGEDGKTPEVTVTPGKDGHSTDITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQG
                     KNGNPSHTIVTFYTDENNDGKYTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKE
                     TKVYQEDPANPGQPLNPEKPLAVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGS
                     QPTTVFVQDGAKGETGATGQDGQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGK
                     NGKDGRAPKIKVEDITSPSRIRRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVL
                     NSKDIYNGIDGRDGSAPTITTKDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITT
                     TENPDGSHTITVTNPDGSTKETVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTN
                     AIIKDGKDGKAATATTTENPDGSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRD
                     NGDGSHTIVITNPEGVTTETTVRDGKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRD
                     GREPKLEVIDNNDGSHTIKVTGADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSD
                     GREYKSIIKDGKDGKDSVSPTVTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPK
                     VSVEDNGDGSHTITIINSDGTVTKTVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVT
                     PSNDKPVPPTPNVPTPEVPVKPVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVP
                     EQPVVPTPAQPATPVNANPVAPTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVG
                     RRKEA"
     gene            complement(88163..88711)
                     /locus_tag="VW325_000087"
     CDS             complement(88163..88711)
                     /locus_tag="VW325_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9733567.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            89032..89520
                     /locus_tag="VW325_000088"
     CDS             89032..89520
                     /locus_tag="VW325_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9733568.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            89517..90704
                     /locus_tag="VW325_000089"
     CDS             89517..90704
                     /locus_tag="VW325_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9733569.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            90697..90921
                     /locus_tag="VW325_000090"
     CDS             90697..90921
                     /locus_tag="VW325_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9733570.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            91078..92424
                     /gene="asnS"
                     /locus_tag="VW325_000091"
     CDS             91078..92424
                     /gene="asnS"
                     /locus_tag="VW325_000091"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9733571.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPVAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            92577..92867
                     /locus_tag="VW325_000092"
     CDS             92577..92867
                     /locus_tag="VW325_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9733572.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            92879..93349
                     /gene="ssbA"
                     /locus_tag="VW325_000093"
     CDS             92879..93349
                     /gene="ssbA"
                     /locus_tag="VW325_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9733573.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVLDFSRNENPFGATNPLDISDDDLPF"
     gene            93381..93620
                     /locus_tag="VW325_000094"
     CDS             93381..93620
                     /locus_tag="VW325_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9733574.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            93915..95315
                     /locus_tag="VW325_000095"
     CDS             93915..95315
                     /locus_tag="VW325_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9733575.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGNPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            95317..95676
                     /locus_tag="VW325_000096"
     CDS             95317..95676
                     /locus_tag="VW325_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9733576.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            95730..96479
                     /locus_tag="VW325_000097"
     CDS             95730..96479
                     /locus_tag="VW325_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9733577.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            96469..96768
                     /locus_tag="VW325_000098"
     CDS             96469..96768
                     /locus_tag="VW325_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9733578.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            96839..97774
                     /locus_tag="VW325_000099"
     CDS             96839..97774
                     /locus_tag="VW325_000099"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9733579.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            97861..98373
                     /locus_tag="VW325_000100"
     CDS             97861..98373
                     /locus_tag="VW325_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9733580.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDEEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            98398..99006
                     /locus_tag="VW325_000101"
     CDS             98398..99006
                     /locus_tag="VW325_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU9733581.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKASLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            99107..99289
                     /locus_tag="VW325_000102"
     CDS             99107..99289
                     /locus_tag="VW325_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9733582.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGLKNVFTK"
     gene            complement(99335..100780)
                     /locus_tag="VW325_000103"
     CDS             complement(99335..100780)
                     /locus_tag="VW325_000103"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9733583.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGVDCYQIIQGKKESYPGDTAVAENYL"
     gene            100886..102508
                     /locus_tag="VW325_000104"
     CDS             100886..102508
                     /locus_tag="VW325_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9733584.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            102711..104669
                     /locus_tag="VW325_000105"
     CDS             102711..104669
                     /locus_tag="VW325_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9733585.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            104841..105074
                     /locus_tag="VW325_000106"
     CDS             104841..105074
                     /locus_tag="VW325_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEU9733586.1"
                     /translation="MKLKLLRVDTKVIMGSFFLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            105080..106522
                     /locus_tag="VW325_000107"
     CDS             105080..106522
                     /locus_tag="VW325_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9733587.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDTSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRITSFETPFALLFI
                     FATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVYSRSKGSSVA
                     LENLLAVPKEKFEGGKSVSGRGLNFNHVYFGYDENRPVLKDIICSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGQSQRLQIARA
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            106680..107774
                     /locus_tag="VW325_000108"
     CDS             106680..107774
                     /locus_tag="VW325_000108"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9733588.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGSKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            107782..108948
                     /locus_tag="VW325_000109"
     CDS             107782..108948
                     /locus_tag="VW325_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9733589.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            109041..112139
                     /locus_tag="VW325_000110"
     CDS             109041..112139
                     /locus_tag="VW325_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9733590.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVDRQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAIQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKTDLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILADNHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVIYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            112187..112804
                     /locus_tag="VW325_000111"
     CDS             112187..112804
                     /locus_tag="VW325_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9733591.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            112815..114149
                     /locus_tag="VW325_000112"
     CDS             112815..114149
                     /locus_tag="VW325_000112"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9733592.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLYQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            114159..114623
                     /locus_tag="VW325_000113"
     CDS             114159..114623
                     /locus_tag="VW325_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9733593.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            114620..115114
                     /locus_tag="VW325_000114"
     CDS             114620..115114
                     /locus_tag="VW325_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9733594.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            115195..116850
                     /gene="mltG"
                     /locus_tag="VW325_000115"
     CDS             115195..116850
                     /gene="mltG"
                     /locus_tag="VW325_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9733595.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            116921..117403
                     /gene="greA"
                     /locus_tag="VW325_000116"
     CDS             116921..117403
                     /gene="greA"
                     /locus_tag="VW325_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9733596.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            117911..118309
                     /locus_tag="VW325_000117"
     CDS             117911..118309
                     /locus_tag="VW325_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9733597.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            118754..119761
                     /locus_tag="VW325_000118"
     CDS             118754..119761
                     /locus_tag="VW325_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9733598.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSKMEIIFIKRF
                     Q"
     gene            119836..120036
                     /locus_tag="VW325_000119"
     CDS             119836..120036
                     /locus_tag="VW325_000119"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9733599.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            120071..120787
                     /gene="atpB"
                     /locus_tag="VW325_000120"
     CDS             120071..120787
                     /gene="atpB"
                     /locus_tag="VW325_000120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9733600.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            120801..121295
                     /gene="atpF"
                     /locus_tag="VW325_000121"
     CDS             120801..121295
                     /gene="atpF"
                     /locus_tag="VW325_000121"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9733601.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            121295..121831
                     /locus_tag="VW325_000122"
     CDS             121295..121831
                     /locus_tag="VW325_000122"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9733602.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            121846..123351
                     /locus_tag="VW325_000123"
     CDS             121846..123351
                     /locus_tag="VW325_000123"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9733603.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            123367..124245
                     /locus_tag="VW325_000124"
     CDS             123367..124245
                     /locus_tag="VW325_000124"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9733604.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            124331..125737
                     /gene="atpD"
                     /locus_tag="VW325_000125"
     CDS             124331..125737
                     /gene="atpD"
                     /locus_tag="VW325_000125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9733605.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            125748..126167
                     /locus_tag="VW325_000126"
     CDS             125748..126167
                     /locus_tag="VW325_000126"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9733606.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(126239..>126667)
                     /locus_tag="VW325_000127"
     CDS             complement(126239..>126667)
                     /locus_tag="VW325_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9733607.1"
                     /translation="RKLSDKHFYRPTFRMHLTNKEILDKLLSYSQDLKHHYQLYQLLL
                     FHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLPYSNAKLEATNN
                     LIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT        38139 a        23461 c        28588 g        36479 t
ORIGIN      
        1 cttggaatta tgcgttttat catggaaaga ttttttataa tagctaaaaa ataatagtta
       61 aatggttatt aattgcattc cctagtgatt tttgttaaga taaatgcaaa tacaaatgaa
      121 agcgagaaca agatgacacg ttatcaagat gatttttatg atgctatcaa tggagaatgg
      181 caacagacag ctgaaatccc agcagataag tctcaaacag gaggttttgt tgatttagac
      241 caggaaattg aagacctgat gttggcgaca acagacaagt ggttagcagg tgaagaagtg
      301 cctgaggatg ctatcttgga aaactttgtc aaataccacc gcctagttcg tgattttgac
      361 aagagagaag ctgacggtat cacacctgtc ttaccactcc ttaaagaatt ccaagaattg
      421 gaaacttttg cggattttac agctaaacta gcagagtttg agcttgcagg aaaaccaaac
      481 ttccttcctt ttggtgtatc gccagacttt atggatgcta gaatcaatgt tctatgggct
      541 agcgctccaa gcacaatctt gccagatacg acctactatg cagaagaaca tcctcagcgc
      601 gaagaactct tgactctttg gaaagaaagc agcgcaaatc tcctcaaggc ttatgatttc
      661 tctgatgaag aaattgaaga cttgctagaa aaaagacttg aattggaccg ccgagttgcg
      721 gcagtggtgc tctctaatga agaaagttca gaatatgcta aactctatca tccatattct
      781 tacgaagatt tcaagaaatt cgcgcctgcc ctacctttgg atgacttctt caaagcagtt
      841 attgggcaat taccagacaa ggttattgta gacgaggaac gtttctggca agcagcagag
      901 caattctaca gtgaggaatc ctggtctctc cttaaagcaa ccttgatttt gagtgttgtc
      961 aatctttcaa ccagctattt aacagaggat atccgtgttt tgtctggtgc ctacagccgt
     1021 gccctttctg gagttccaga ggcaaaagat aaggtcaaag cagcttatca tctagcacaa
     1081 gaacctttca agcaagccct ggggctttgg tacgcccgtg agaagttctc tccagaagcc
     1141 aaggcggatg tggagaaaaa agtggcaacc atgattgatg tctataagga gcgtctgctt
     1201 aagaatgact ggctcactcc agaaacctgt aaacaggcta tcgtgaagct caatgtgatc
     1261 aaaccttata ttggctatcc agaagaattg cctgcacgtt acaaggataa ggtagtgaat
     1321 gaaactgcca gtctttttga gaatgctcta gcctttgcgc gtgtggaaat caagcacagt
     1381 tggagtaagt ggaaccagcc tgtagactat aaggaatggg gcatgcctgc tcatatggtc
     1441 aatgcctact acaatcctca gaagaacctg attgtctttc cagcggccat tttacaggcg
     1501 cctttctatg acttgcatca gtcatcttct gctaactacg gtggtattgg ggcagtgatt
     1561 gcccatgaaa tttcccacgc ctttgatact aacggggctt cctttgacga aaatggtagc
     1621 ctcaaggatt ggtggacaga gagcgactat gctgccttca aggagaaaac acaaaaagtc
     1681 attgaccaat ttgatggaca ggattcttat ggagcaacca ttaacggtaa attgactgta
     1741 tcagaaaacg tggctgactt gggaggaatc gcagcagcgc ttgaagcagc taagagagaa
     1801 gcagacttct cagcagaaga gttcttctac aacttcggtc gcatctggcg catgaaaggt
     1861 cgaccagaat ttatgaaact tttggctagc gtcgatgtgc acgcaccagc caaactccgt
     1921 gtcaatgtgc aagtaccaaa cttcgacgat ttctttacaa cctatgatgt caaagaagga
     1981 gacggaatgt ggcgttcacc agaggagcgc gtgattattt ggtaatacaa aaaatctagt
     2041 catcagaaaa aaggcatcca atcatgtgtg aaaaccttga taggatgcct tttgtaatag
     2101 aaagatttgc tggatagttt acttaaattt cgattgtcat catcttgcta gaaaaaggga
     2161 ttgaaggcct tttcgtgagc gatagtagta gctggaccat gtcctggata gacatcgtag
     2221 tttggtaggg tgaagagttg ggtctggata ctatgaagga gttgctccat gctaccagtc
     2281 ggaaggtcgg tccgtccgat agtttcgcgg aatagagcat ctcccgtcaa gactaggtga
     2341 gcatcaggaa agactaggga aacaccaccg atagagtgcc ctggggtcgg tagaacctta
     2401 aaacgaaatt cctctagttg gtattcttcg tgaaagacaa aggtgtgttc tgcaggtttt
     2461 gtgaccacat ctgccatatc atcgtggcga gggagaccgg agagattatc gacaggagtg
     2521 tagagccagc tggcttcgct ctctgcgata tagacaggag gattgccaaa cgtctcgcga
     2581 accaagtcca gactcatgat atggtcataa tgggcgtggg tcaagagaat agcacagatc
     2641 ggtttgttga tcttctcgat tgtctgacga atggcttccc aatgactacc aggatcgacg
     2701 acgatgaggt gcttttcgcc ttctagataa taggtatttt cataggcaac aggattcacg
     2761 gttttatgga ttttcatact agctccaatc tcaaagaatg tactaatact cttcgaaaat
     2821 ctcttcaaac cgcgtcaact ttatctgcaa cctcaaagca gtgctttgaa cagcctgcgg
     2881 ctagctttct agtttgctct ttgattttca ttgagtataa attaagtata gcacagttag
     2941 ggagaatagg taaggattta aaatgaactt aaaaaccagc ataactagat gaagacaggt
     3001 tggttatcaa tttatcaata ttggaaaggt gtcaattgcc cttcataggt tgcataaact
     3061 ccgtcattag cttgtttgat agacgtgatg tttagagtgc taccgtagtt ggcttctcat
     3121 ttgtcgctat attcactata agttatccta ctgggcaggt tctggtcgtt agcatagtat
     3181 tgaacgagtt ttttatagct ttgctggctg aataggaaca ggcagcaaac tcctaacatt
     3241 aggagagcaa agatagaaat agcagttttt tcataggtat tctccttata aactaaagga
     3301 tagaagaaag tatcttgagg atttttatac tcttcaaaaa tctcttcaaa ccgcgtcaac
     3361 gtcgccttgc cgtatatatg tgactgactt tgtcagtctt atctacaacc tcaaagcagt
     3421 gctttgaaca gcttgcggct agtttcctag tttgctcttg gattttcatt gagtattaat
     3481 tccaaaatcc agtcaaggta ccagtgtatg tgatacgagt tccaccgttt atctttcgta
     3541 tcttcaatgg aattggggaa gtcagaaagt ctttgggtta caatatggtt tccgttcttc
     3601 tatctcttct tctatcacct aagtttaaaa tctaacttcg tagtatatta tgggccaata
     3661 attcagaatt atcaagcaat tttgaattat ttttttctta ttctgttcca atctagtttt
     3721 catattctta aaaaagtgat aaaatggtag gaagaattgg agagtagaga tgccgaaaga
     3781 agtgaattta acaggcgaag aagttgtcgc tttaaccaaa gaatatttaa cggaagagga
     3841 tgttcatttt gtccataagg ccttgatcta tgctgttgaa tgccacagtg gtcaatatcg
     3901 caaatcaggc gagccttata tcattcaccc tatccaagtg gcaggtattt tagctaagct
     3961 aaagctggat gctgtaacag tagcttgtgg attcttgcat gatgtggtgg aagatacaga
     4021 tgcgaccttg gacgatttgg aaagagagtt tggtcctgat gtgcgggtga ttgttgacgg
     4081 agttaccaag cttggcaagg tcgagtacaa atcgatcgag gagcaattag cggaaaatca
     4141 tcgcaagatg ctcatggcca tgtctgagga catccgcgtt attttggtca aactgtctga
     4201 ccgcttgcac aatatgcgga ccctgaaaca tcttcgaaaa gacaagcagg agcgtatttc
     4261 caaagaaacc atggaaatct atgccccact tgcccatcgt ttggggattt ccagtgtcaa
     4321 atgggaatta gaagacttgt ctttccgtta tctcaatcca acggagtttt acaagattac
     4381 ccatatgatg aaggaaaagc gcagggagcg tgaggccttg gtggatgagg tagtcacaaa
     4441 attagaggag tatacgacag aacgtcactt gaaagggaag atttatggtc gtcccaagca
     4501 tatttactca attttccgca aaatgcagga caagagaaaa cggtttgagg aaatctatga
     4561 tctgattgct attcgttgta ttttagatac ccaaagtgat gtttatgcca tgcttggtta
     4621 cgtgcatgaa ttttggaaac cgatgccagg tcgcttcaaa gactatattg ccaaccgcaa
     4681 ggccaatggt tatcagtcta tccatacgac tgtttatgga ccaaaagggc cgattgaatt
     4741 ccagattcga accaaggaaa tgcacgaggt ggctgagtac ggggttgcgg ctcactgggc
     4801 ttataagaaa ggtataaagg ggcaagttaa cagcaaggaa tcagctattg gaatgaactg
     4861 gatcaaggag atgatggagc tccaagacca ggctgatgat gctaaggaat ttgtggactc
     4921 tgttaaggaa aactatctgg ctgaggagat ttacgttttt accccagatg gagctgtccg
     4981 ttcccttccc aaagattcag gaccgattga ttttgcctac gaaatccata ccaaggtcgg
     5041 tgaaaaagca actggtgcca aggtcaatgg ccgcatggtt ccactgacaa ccaagttaaa
     5101 gacaggggat caggttgaaa ttatcgccaa cccgaactcc tttggaccta gccgtgactg
     5161 gctcaatatg gtcaagacta gcaaggcgcg caataagatt cgccagttct ttaaaaacca
     5221 agataaggaa ttgtctgtca acaagggtcg tgagatgctg atggctcagt tccaagaaaa
     5281 tggctatgtg gcaaataaat ttatggacaa gcgccacatg gatcaagttc tgcaaaagac
     5341 cagttacaag acagaagact ccctctttgc ggccattggt tttggggaaa tcggtgcgat
     5401 taccgtcttt aaccgtctga ctgaaaagga acgccgtgag gaagagcgtg ccaaggccaa
     5461 ggctgaggca gaggagcttg tcaaaggtgg cgaggtcaag gttgaaaata aagaaactct
     5521 caaggtcaag catgaggggg gagtggttat tgaaggtgct tctggtctcc tagtgcggat
     5581 tgctaagtgt tgtaaccccg tgcctggtga cgatattgtt ggctacatta ccaagggtcg
     5641 tggtgtggct attcaccgtg tggactgtat gaacctgcgt gcccaagaaa actacgagca
     5701 acgtctcctt gatgtggaat gggaagacca gtactctagc tcaaataagg agtatatggc
     5761 ccatatcgat atctacggtc tcaaccgtac aggactgttg aacgatgtac tgcaagttct
     5821 ttcaaataca accaagaata tttcaacggt caatgcccaa ccaaccaagg atatgaagtt
     5881 tgctaatatc catgtgtcct tcggtattgc caacctctct acactgacca cggttgtcga
     5941 taaaattaag agtgtgccag aagtttactc tgtcaaacgg accaacggct agttgtccta
     6001 gctcttacta gaaaggctat tatgaaaatc attatccaac gggttaaaaa agcccaagtg
     6061 agtatagaag gccagattca gggaaaaatc aatcagggac ttttattgct agttggtgtt
     6121 ggaccagagg accaagagga agatttggac tatgctgtga gaaaactggt caatatgcgg
     6181 attttttcag acgcagaagg caagatgaac ctgtctgtca aagatattga aggagaaatc
     6241 ctctctattt ctcagtttac cctctttgcg gatactaaga gaggcaatcg tccagccttt
     6301 acaggggcag ctaaacctga tatggcatca gacttctatg atgctttcaa tcaaaaatta
     6361 gcgcaagaag tgcccgttca gacagatatc tttggagcag atatgcaggt tgagctggtt
     6421 aataacggac ccgttaccat tatcctagat actaaaaaga gataaaaaag accaagccca
     6481 gtcggcttgg tctttctcat ctatcataaa atactccaaa aagaaatcgg ttcttgatat
     6541 gcttggggga agtctctttt caggctttgg cagatgcgat aggaagggat gagatgtcct
     6601 aggatgagga gagttccctg aaggaaaagt ggaatatcac ttaaagacac caaggagaga
     6661 atcatcaggc tatcccatag gagaatttgt aaagcaaaac gccagaatct cggtctaatc
     6721 tgggaataga gctgactaaa gtggtcacaa agttggtttg aaagataggt caatagcaca
     6781 ggatgaaaga aaatgagcca accgctataa atcaaaatcc agtcccaagt aagcccccct
     6841 ttttaaatgt caaaaatgcc agcatgattc catcaatgac aaggagttga atggttttga
     6901 tgaagatgat ttttttcatg ctaaaacctc cttttcctta gagacactcc cctacaggta
     6961 ttttagaatg tatattgctt agatattcct tattttatta gctttttaag gtattaaagt
     7021 agttatataa gtttatgaag ggtatcctat cctgcacggc taattttgag ttcgaaactg
     7081 aggagtttat aactcaaaat ttcgattaaa tcagcattta acgatagtat gtttgtcata
     7141 aaatgataaa tttttagatt gtggaaattt tttttaactt aattcttgat ttttttctaa
     7201 atgtgatata cttaatttac gggataaaaa aaccacctta gaaaggtgtg ataatgtgca
     7261 aaaagagaat gttagaatgc gtttagcgtc tgaatccaga cagatattag ataagcttaa
     7321 agatgatgtt tacaataaat taggctatga agtaagctat tccagtattg ttagtcaagc
     7381 tgttagagaa tacgttccta aaaaagaacg tatagattgg ataaaattaa aagagacagc
     7441 tatacccttt tcatcgttaa aacagagcaa taattgggag taccagacat cacttatgtt
     7501 agaggaagat gtattaatct tactgtctga actacaaaat tttttcttag atgtcttcca
     7561 agcgaaacgt atacataggg cattttgtgt acgcctatgc ttgaaagcac aatttttatt
     7621 aagtaataat gattcttaat actatcaata ttaatgcgga gtgtaatgct ccgctaaaat
     7681 aaaacctatt acgggataaa aacactactt tatttaggat ggaaagagga acgaatgaag
     7741 atgaaagcta gagattcacc attgaattga ttttgttttt agaaaaaatc aataattaga
     7801 aaaaatacga ggtaaaaaaa tgaatagaga tgtattagag tttctgagaa cagagaccgc
     7861 tgaaaaaatt agtctttata taagcgaggc taatagattg gagggcgatg taactctact
     7921 cgcacctagt tctcaagatt tggaagatat taaaaatgct atgctttcca attctaatct
     7981 cggattaaaa gttgcgagac tggatgtgat gaagaaaata gcatatgctt ctacaagaaa
     8041 tcattattta acaggggcta cgatttttgg ggatatttcg aaaggaacat acaattgtga
     8101 tcctaaaagt tatgtctaat ataagggagg ttaatccctc cccttgtttt tgaaaatcga
     8161 aaggaaaagt ataaaaatga aagttaatga atatttctgg agaacgagct gtcttatact
     8221 aaaaaagaag aaatcgataa aatagtatac gtgttagaga tgtgtttgtc gtaaaagtct
     8281 ggatagtttt ctcctaggta gtcagctgtc atataataaa aagtagaatg gcaagcagtg
     8341 ataattggca taagagatga aaaacgctga gaactaatga aggctaagag aacaaaaaat
     8401 agaaaatcaa tggtgagaac ccctaaatag tagaagcgaa tctttttatt gatttgagga
     8461 taaatctcag cgaattgatg tttgagtcgg acgaccaaga gaaatagcag gagtccttga
     8521 gcaaggaaga aaattaaacc agagagcaag agccactcaa aagatgtctc gagtctagtg
     8581 ataaaaaatg caaatagtag caaatcaata actgcaattg ctataaaatg gattctatag
     8641 agttttttca tgacaagacc ttcctctttt atctaacttc attctactcc aaaagaatgg
     8701 gagttacaac taaaatgata aaaatagcag aagggagatt ctcttaagtt ggctagtatt
     8761 ctttatttga gtttccttct atgtatctaa cttcttcatc attccagaca aataaagctc
     8821 cgattgcatt gaggatataa aagatgtatt taccgatatt ggcgaagttt ccttgaatac
     8881 cagcttttgt cagctgaacg aaattgtaaa tcaaccaaaa gccccactga gttgttagtt
     8941 tcaatgcatt caaagcattg gcaatgaggg acagtgcaaa ggcaatagtt gttacgtagg
     9001 caaggagatt catcttgccc tcatatccga tatagttggt cacaaaggca aagaggaagg
     9061 cgatgatgga aatgatgatg gccgccaatt ttacctgttt ttggctcatt tggttgggtc
     9121 tgccttcttg cgaagctttc catttcttta tagcaaaggt ataaatgagg aaggtgacgg
     9181 gataggtaat gatggccgcc ttatttccaa ggatataatc aatagcaccg gacaaaatgg
     9241 tattaacaat accaaagtaa tttccccatt tgcttaattt ccccgtgaaa cgagtggaca
     9301 acatggaaat ctcaacgttg gttacggaaa tcaatccaaa gggtacaaga gctgtccatg
     9361 gtccccagtc tacaaattta tcgaggcgtg agttgaggta accagatgca atcgcaatcc
     9421 aacgaccaaa gcaaccccga agaggtcaaa ctatttagat gtagcaaaaa tttttagtga
     9481 ttttttcata ggttaaacta cctttctttt tttcaaatat tctcccacca aatgaaagta
     9541 aaataaaatg atagaaataa aaccctgaaa ataaaacttt cagggttggt aggggacttg
     9601 agaaattagt tgattttctc gacatagagt tgttttgcaa tgtccatatt cacttgtaaa
     9661 tcctcgtcgt tactgaggat agtgaaggta ttgctgaagc catcaaactg cttgacttgg
     9721 agctggtcac cgatgtgaag tgagtgcttg tccagataat ggagaatgtc aaaattatcg
     9781 tgcacccgag tcaggcggta ggcgccagct tccttgatat cagctagtgg gaggttattg
     9841 atttcaacga ggagttctcc cttggcagga atagttcctc cgtgggggca ggttttaggg
     9901 aaacctagca gtttatctag tctttccacg aacaggtcag agacagtgtg ttccaagacc
     9961 tcagcttcct cgtgaatctg gtcacttgta tagtctaaat gatgaactag aaaaacttca
    10021 atcaagcggt gcttacgata gagctcagag actagtttga gaccgaggtc agtcagtaga
    10081 tagccacatt ccttgtcctt taggatgagg ttttcacttt tcattcgttt gatcatttca
    10141 gttacggcag ggggagagac ttgcatgcga gccgcaattt ccttgttggt aatcttatgc
    10201 aggtctatgc caatttcata aatacatttt agatagtctt ctttgtttgg ggtcattcgt
    10261 ttcctcttgt ctaatatctt tatctagtat atcaaaaaaa gctagatttc tctagctgtg
    10321 actttttatg ttatacttat tgcaagagaa aaaacgagga gatggatatg acgaaaatag
    10381 ctcttctttc agatattcat ggaaatacca ccgccttgga ggctgttttg gcagatgctc
    10441 ggcagctagg agtggatgaa tactggcttt tgggagatat tctcatgcca gggacaggac
    10501 gtagaaggat tttggacttg ttggatcaac taccgattac ggctagagtt ttgggaaact
    10561 gggaagacag tctttggcat ggtgtccgta aggaattgga tagtactcgc cccagtcaac
    10621 gctatctctt gcgccagtgc cagtatgttt tagaggaaat ttccctagaa gaaattgaag
    10681 tgctccacaa tcaacctctt caaattcatc gtcagtttgg ggatttgacg gtgggaatta
    10741 gtcatcatct gcctgataag aactgggggc gagagttgat tcatactggc aaacaagagg
    10801 attttgaccg cttggtgact catcctccct gtgatattgc tgtttatgga catattcacc
    10861 agcagttgct tcgttacggg actggtgggc aattgattgt caatccgggt tcgattggcc
    10921 aacctttctt tttggacgct cagctacgga aggacttgcg ggcccagtat atgattttgg
    10981 agtttgatga caagggcctg gtagatatga acttccgacg ggtagactac gatgtagcag
    11041 ctgaattgca gctggctaaa gacctgagac ttccctattt tgaggtttac tatgaaagtc
    11101 tggtcaatgg gatccaccat actcatcatc aggaattttt gagagaattg gctcagaaag
    11161 agggttgcga tcgggagtta gacgactggt tgaaaagtgg taacgattga cattacaact
    11221 aaaaagggta taataataga aaaagaagag tagaggcagg gtagcctcgt aaaaaggaga
    11281 agaggatgca aattccaagt agatttacca ttgcgactca tatgctgata atcattgccc
    11341 tcgaggggaa ggaaagcaag gtgaccagtg attttctggc tgctagtgtc ggggtcaatc
    11401 ctgtcattat cagaaagatc ttgtcccagt tgaagaaggc agagctgatt tcagtagcgc
    11461 gtggaacggg cggaacagag attgtcaagg accttaagga tattagtctt ttagatgttt
    11521 atcaggcggt cgaatgtctt ggtaagacag gtcaactctt cagtttccat gacaatccga
    11581 atccaaattg ccctgtagga gctcatattc atgatgtttt ggatcaaaaa ttggagagaa
    11641 ttcagttgac tatggaggca gaacttggtc agaccagtct agaaaaagtc gtggccgatg
    11701 cagagagtca gatgaaggat taagagggat aaatgccctc tttttttctt tggataatta
    11761 tgataaaatg tagctattaa aggagtaaga aatgtatctc gttataggaa ttgtattagc
    11821 ctttatagtg tcattttgga aggataatag aagtttgtgg aatccagtat tatttttatt
    11881 atccctcatt tcaagttatt tttatctatc ctaccttttt tataaaaatg gatacgagaa
    11941 tgttcagtta gatttttatg tatttgcctt tgtcttactt ccttttttgc tttttctaag
    12001 tggcatcttt ttaatctata atggggtcat tttgttgaag cgagagggac gttccaagcc
    12061 ccattatctc tcaatgctct ttgattttta ttgactatta gattctattt aaaaagactt
    12121 agaatgcaca tgaaagtatg tattctaggt cttttgtttt gtatacattt aattttcaag
    12181 agtattatct atttttccat gcctggtaac gagtatcaat caataactgg gtaaggcgac
    12241 ccaaggtttc tctttcttct ggagtgaggg attgagcttg gacaaagaga tgatgaatgt
    12301 attcaagcgc ctttaaggaa gtcaagtcat tgatagagct aataccagca tcaagaatag
    12361 tgccaaagaa gaggtcgaag tagagctgaa gttctgcgtc agggactgtg gccacccatt
    12421 ctttaaaggt tgtgtctact tgctggctat cactgttggt cttatccagt tggacgaagt
    12481 gcttgtcctc aatctgccaa ctaaaggtat cgtgctgggc gatgccaccc agggcagtac
    12541 tctgaacgat gatttggtga gcaggaattt ccagcatcat accgataatg gaaccttgtg
    12601 gaatgaatat cttgcttcta tccattatcc tttgataacc cgcagtctgt gtcaattctt
    12661 gatggagacc aggtgcatca aatgtataaa ctgctgtgat ctgattttgc aaactttgct
    12721 caatttggct agcagcatag atagcgagat ttcctccctt ggaatgccca gccagaataa
    12781 ccttttgctt aggatgatgg gcaaaaaagt tctttaaata gcgaagggcg tgcttttgag
    12841 caggaatttc cttcatatag gtcaggtgga aatcttcctt ccagccaatg atactgtcat
    12901 ctgtcccacg aaagacaatc agataggtat cgaggctgac acgataagtc atagccgcaa
    12961 attgcttttg cagttcaggg tcgatgtcgt tgataaaatg ggagagtttg caatttttga
    13021 agcgcttgtg ttgagccaat tcatctaata attgaaggcg atttttgcta gtaagcatgg
    13081 tgggctctct tggaacctga ggtgctagat ctaaaagacg ttgaggaagt gtggagacca
    13141 gattatcaaa ggagaggtag gtgatttcta ttaaggttag aatgtctaac tcattcaagg
    13201 gaaggtcgta ataagaatca tatgcgacat ctttcagata gtcaaaaata ttggccataa
    13261 gagctccttt ctttttattt atcttagcaa atttccctca aattagctag tagcattgcc
    13321 tgtttgtact ggctaaaaac aggctatttc aaattcagtt tcagaccatc tggcatggaa
    13381 aaatctgtta taataataga aaaggagaag cgcatgcaca agattttatt aatagaagat
    13441 gatcaggtca ttcgtcaaca ggtcgggaaa atgctctctg aatggggatt tgaagtggtc
    13501 ctggtagaag actttatgga agttttgagt ctatttgttc agtcggaacc tcatctggtc
    13561 ctcatggata ttggtttgcc cttgtttaat ggttatcact ggtgtcagga aatccgcaag
    13621 atttccaagg tacctatcat gtttctttct tcgagagacc aggctatgga tattgtcatg
    13681 gcaatcaata tgggggcgga tgactttgtg accaagcctt ttgaccagca ggttctttta
    13741 gctaaggttc agggcttgtt gcgtcgttcc tatgagtttg ggcgtgatga gagtttgctg
    13801 gaatatgctg gtgttatcct caataccaaa tccatggatt tacattatca agggcaagtc
    13861 ttgaatttga ccaagaatga attccagatt ttacgcgtgt tatttgagca tgcaggcaac
    13921 atcgtagcac gtgacgacct gatgcgggaa ctttggaaca gtgacttttt cattgatgat
    13981 aataccctct ctgtcaatgt ggctcgtttg cgtaaaaagt tggaggagca gggattggta
    14041 ggatttatcg agaccaagaa aggaataggg tacggattga agcatgcttg attggaaaca
    14101 attttttcta gcctatctgc gctcccgtag tcgtcttttt atctatctgc tttctttggc
    14161 atttcttgtc ttactctttc agtttttatt tgccagtcta ggaatttact tcctctactt
    14221 tttcttcttg tgttgctttg taaccatctt atttttcact tgggacatat tggtggaaac
    14281 gcaggtctat cgccaggaac ttctctatgg agagagggaa gccaagtctc ctttggaaat
    14341 agctttagca gaaaaattag aagcgcgtga gatggaactc tatcagcaga ggtcaaaagc
    14401 agaaagaaaa ctgacggatt tgctggatta ctataccttg tgggtccatc agataaagac
    14461 ccccattgca gccagtcaac tcttagttgc agaagtggtc gaccgccaac tgaagcagca
    14521 gctagaacag gaaattttca aaatcgactc ctataccaac ctagttttac agtacctgcg
    14581 tttagaaagt ttccatgatg atttgctctt aaagcaggtt caaattgagg acttggtcaa
    14641 ggaaataatt cgtaaatatg ctcttttctt tattcaaaaa ggcttaaatg tcaatctaca
    14701 tgaccttgat aaagaaatcg tgacggataa aaagtggctg ctagtggtta ttgagcaaat
    14761 catctcaaac agtctcaagt acaccaagga aggtggtctg gagatttata tggatgacca
    14821 agagctttgt atcaaagata cgggaatcgg gataaaaaac agtgatgtcc tccgagtatt
    14881 tgaacgtggc ttttcaggat acaatggccg tttgacccag cagtcctctg gacttggcct
    14941 ttatctatct aagaaaattt ctgaagaact ggggcaccag attcgtatcg agtctgaggt
    15001 cggaaaagga acgacagtgc ggattcagtt tgctcaagtg aacttagtcc ttgagtaaaa
    15061 atagatgaga aaaagcctcc agattggagg cttttatgct agactcttat ttctcaacgc
    15121 gtgatttgtt ggcgatatca gctaggatag cttgaacaaa attatcaact gagacagttt
    15181 gtgtttcttt ttggccgtag cgacgaacgt tgactgtttc gtcttccatt tctttgtctc
    15241 caacaattaa ttggtaagga atcttgctgg tttgtgaagc acggatcttg aactgcattt
    15301 tttcattgcg ctcatctacg tctgcacgga caccgcggtc acggagtttc ttggccactt
    15361 cccaagcgta gtccacgtgt ttttcgttag atactgggat gagggttact tggtgtggtg
    15421 ccagccatgt tgggaaggcc cccttgtagt tctcaatcaa gatagctgtg aagcgttcca
    15481 tagttgagat aaccccacgg tggatcatga ctggacggtg atcttcgcca tcagctccga
    15541 tgtatttgag gtcgaagcgt tctggcaaca agaaatcaag ttggatagta gaaagggttt
    15601 cttcttttcc aagggcagtt ttaatctgga tatccaattt tggtccgtag aaggctgctt
    15661 caccttcggc ttcaaagtag tccacgccca tttcatcaag agctgcacga agcatggttt
    15721 gggcattttc ccacatctca tcgttatcaa agtacttatg agtatcttga gggtcacgaa
    15781 gagagaggcg gaagcggtag tcagtcaagt tgaagtcttc ataaacatcg ataatcaact
    15841 gaagggcacg ttggaattct tcttggattt gttctggagt aacgaatagg tgaccgtcgt
    15901 tgagtgacat ttcacgtaca cgttgaaggc cagtgagggc accagatttt tcgtaacggt
    15961 gcatcatacc gatttcagcg atacggattg gcaattcacg gtaagagtga acatggtgtt
    16021 tgaaaacttg gatgtggtgc ggacagttca ttggacgaag gacaaattct tccccgtcac
    16081 ccatgtccat ggttgggaac atgtcttctt ggtaatgatc ccagtgacca gaagtcttgt
    16141 aaagctcaac agaagcaagt ggtggagtgt agacgtgttg gtagccagaa gccaactctt
    16201 tgtttacgat gtagtgttcc aattcacgac ggatagtcgc accatttggc aaccagaatg
    16261 gcaaaccttg tcccacttct tgtgaaatca taaagaggtc aagctcttta ccaagtttac
    16321 ggtggtcacg ttccttagct tcttcacgca tttgaaggta gtttttcaag tctttcttgt
    16381 caaaccaagc tgtaccgtag atacgttgca tcatagcgtt gtcgctgttt ccacgccagt
    16441 acgcaccagc tacatggaga aggtggaaga tttggatacg acctgttgat ggaacgtgag
    16501 gtccacggca gaggtctaca tattcaccct gacgatagat agtcaaaccg ccttcgtctt
    16561 ctgagtgttc ttcaatcaat tccaacttgt aagggtcatt tttgaagatt tcacgtgcct
    16621 cgtctttagt cacttcttca cgaatagatg ggaagttttc tttgacgatt ttttgcattt
    16681 cttcttcgat acgaggaagg tcttcgttag agatttgacc agctgtgttg tcagtatcgt
    16741 agtagaaacc atcttcgatg gctggaccaa ctcccaagtg aatgtctggg aaaagacgac
    16801 gagctgcttg ggcgaacaag tgagctgctg agtgacgcaa gattggaagg gcatcttcgt
    16861 gatcaggtgt cacaatttcg atgcttccat cttcagtgat agcgcgagta gtgtcgatga
    16921 gtttgccgtt gaatttacca gccaaggctt ttttagctag ggaattgctg atagattggg
    16981 caatttcaaa agttgttacg ccagattcga attcacgaac agcgccatct gggaaagtaa
    17041 tgttaatcat gatgttctcc ttttttatta tttcatagtt tctaagcatt gattttttat
    17101 cagcagacta cttccgagtc ctaacgttca attgcttttc tttatcagag aggaagtttt
    17161 ccatttccgt cagaatgtct atatccaaac gttgagaaag ttctaatagc caccagatat
    17221 tttctgaaag tttttgttcc agtgtgtagg atgtttcatc atagtagcgt ccttgctttg
    17281 tcatcaccag tcattggaaa tttccaatat cattagataa agccaagagg tcttcttcta
    17341 ccgtccactt gaaatcatga tgcttaactt ccagttcgtg ataagcttgt cggattgccc
    17401 aactgcgctc cactaattct tgcaactcca taattagtat cctaataaaa aaatgcgaca
    17461 tcctcgaaag gacgtcgcaa cgtggttcca ccttcattta tgtacctcaa aagagaggga
    17521 cacctctgat cggctctaac gtggccaccg ttttatgttt tcataaaaaa ctcaagagta
    17581 gtatcagttt agacttccta tgcgtttcca gcaaccacgc actctctagt agaaagggac
    17641 taagtgactt gtctctatgg attattatag catgattgtg agatttttca aggattttgt
    17701 gaaagttttt tgtttttcat tttttgtaga atatgcttga tgctaatacc agaatccatg
    17761 cccaccagag caactgtttc atagattagc aagtaaatta gtataaactc accaaaggtt
    17821 atgatagtaa agtaaaagag cagtccgtta ccaactaacc atcaaagtga gaaacggaaa
    17881 cggtagctat agagtaacga tacaatcaag attaccatag gtgtaaagaa gagaagattg
    17941 tttatataga gtcccttgag tgtaaggggg tcagaaacaa ctagcaagaa caactcataa
    18001 agtggccaat aaaagaataa tatgaccaga tagtagggga gatgggaata gaatctacgc
    18061 atgataatca cctcgtttta aaaaaacaga caacttagat ctcttgctat gattattata
    18121 gcatgtttaa aacaagaaag taaatttgtt gacaaagaag atttttcttt tcactagaat
    18181 catagttagt tttcttggta gaaatatttt gataataatt aagcaagact attgatattg
    18241 attattaaga gtgtcacaat tgttgtaact aaaatgatta caacaataga ggtataaaaa
    18301 atgaaactag cagttattgc agcaaatgga caagcgggga aagcaattgt tgaagaagca
    18361 gtcaaacgag gacatgaagt aacggctatt gttcgttctg aaaacaaaag tcaagctgag
    18421 tctattatta aaaaagattt atttgagttg acaaaagatg atcttactgg atttgatgct
    18481 gttatttcag cgtttggtgc ttatacacca gatacgctac cgctccacag caaatcaatc
    18541 gaactattta atcagcttct tgctggaaca cagacacgtt ttctagttgt agggggagca
    18601 ggaagtcttt atatagatga aacaaaaacg acacgacttt tagatacgtc tgattttcct
    18661 gaagaattta agtcactagc aaaagatcaa gcggatgaat tggatcttct tcgtacaaaa
    18721 aataatctca actggacttt tgtcagttct gctgtagatt ttattcccga tggtgaaaaa
    18781 acaggaaact acattctagc aggagaaatt tttactacta atgaaaaagg aataagccag
    18841 attagttatg cagattatgc tattggattg gtagatgaac ttgaaaaagg tcatcatata
    18901 aaagaacata tctctttact tgaaaaataa taactatgag agtattaatg tttgacagaa
    18961 aatttaaatc ttggtaaaat agggattgtc gtaaagacaa ataacttctt cttggttaca
    19021 ggcatgccaa cctgtcactc ggatgaagcc aaataaaaag gagaaacatc atggcaatct
    19081 caaaagagaa aaaaaatgaa atcattgcac aatatgcacg tcacgaaggt gatacaggtt
    19141 cagtagaggt tcaagttgct gtccttactt gggaaatcaa ccaccttaac gaacacatca
    19201 aacaacacaa aaaagaccac gctacttacc gtggattgat gaaaaaaatc ggtcgccgtc
    19261 gtaacttgct tgcatacttg cgtaaaaacg acgttaaccg ttaccgtgag ttgatcaact
    19321 ctctaggact tcgtcgctaa ttcaagatac aaatgctgtc aaaagcacaa agcaaaaata
    19381 ggaaaattga cgaagaaact tcagtttcta ggagatttta tcttttttgc caagtgctta
    19441 ggccgtgttc aattgagcat atcttgataa tgaagctact ctaaattggg tagctttttt
    19501 gcgttgtcac cttacctcga tatactcaag tataatcttt ggttacggtt cctagcactg
    19561 taaggtaaaa taaaccagct tttctccctt cggggagatt ttttttgttt cactgaaagt
    19621 ttcgtacgat cttcgcctcg tcgcctagtc tataaacgat ttatctagca agaatcatga
    19681 tgctaagggc gtcaaaaatc cgtatgaaaa tagggaaatg acacagtgtt cgatgagcac
    19741 aaggagtttc atctttttca ctaggatttt agcccgggct caaatcagct ctctgacttc
    19801 agagggcttt tttattgagg ttttatcggt aacaattttg gagactacaa aaacctcaaa
    19861 cggtatcagc taattacacc ataaaggtgc gcagttactc ggcttttcaa gccgagtagc
    19921 tgtatgcaag ccccctagaa gagcccactc tttacaaagt aaagtatagt atgttatact
    19981 ttacatggaa gtagtcactg aattccagtt agaaattact ttgtaactac gttttgagga
    20041 ggagtaaaat gctttcctac gttcgacatt acccactagc gatagctaaa ttaatgtgtc
    20101 tgtgctctcc taaaatctgc tgatttatta ctgactaata caggaggttt tttttatggg
    20161 acagacaatc atatctgcta ttggtgttta tatttccacc agtatcgatt atttaattat
    20221 tttaattatt ttatttgcac agctatcaca gaataaacag aaatggcata tttatgcggg
    20281 gcaatatcta ggcacaggct tacttgtagg ggcgagttta gttgctgctt atgtcgttaa
    20341 tttcgtgcct gaagaatgga tggttggatt gcttggttta atccctatct atttagggat
    20401 tcgctttgca attgttggag aagatgcgga agaagaagag gaagaaatta ttgaaagatt
    20461 agaacaaagc aaggcaaatc aactgttttg gacagttaca ttgctgacaa ttgcgtctgg
    20521 cggagataat ttaggtatct atatacctta ttttgcttcg ttagattggt cacagaccct
    20581 cgtggccttg cttgtgtttg taatcggcat aattatcttt tgcgagatta gtagggtgtt
    20641 atcctctatt ccgttaatat tcgagacaat tgaaaaatac gagcgaatca ttgtgccctt
    20701 agtattcatt ctacttggac tatacatcat gtatgaaaat ggcacgatag agacttttct
    20761 gatcgtgtag atttttttgt ttcactagga ttttagcccg agctcaaatc agctctctga
    20821 ttttcagagg gctttttcat tgagtataaa tggacaatct taaagttact ctattgtaga
    20881 gtggcttttg atttgattag gagattctta taaatatagt aaaatgaaat aagaatagga
    20941 caaatcgatc aggacagtca aatcgatttc taaaaatgtt ttagaagtag aggtgtacta
    21001 ttctagttcc aatctactat ataaatgaaa accttagctg tttggccaag gttcatttct
    21061 tctatgcaag ttcttctctt ttcttatctg ggttccagat gaagcttgcg ataaagctaa
    21121 agatgatggt taggatagcg aggataatag caaggatgag ggcagggatg cggataaacc
    21181 accagagtgg atttggtttt ttatcattgt gccattgttt cgtttcttcg tccagacgtt
    21241 ggaattctgt ttgaatacga ttggcgactg tttcaatgcc ttcatcattc attttcttga
    21301 tatctgagat atcgattgga tttccaaagt tcatatcgac acgttcacgg ctaatcaagc
    21361 ccttcaaagt catgggaccg gtgtaggtaa ccggcatgat acggaccttg gccattttgg
    21421 caatcagtgc tgcgcccccc ttgacatcgt ttgagtggcg gctaccactt ggaaacatga
    21481 tgagagagcg gtcacttttt ttgagaacgt tgataggata tttgatggct gaggcgctgg
    21541 gattttcacg gtcgatggga aaggcgccac acatacgaat ccaccaacca aagatacggt
    21601 tggtaaagag ttcttttttt gccataaaga tgaactgttt tggcttggtc gcaaaggcca
    21661 tataaacagg atcccaccag gtacggtgag gcgcaactaa aatataattt tcatcttgat
    21721 taggaatttt atcagtatta tgatagtgag cattgccatt gatggaccat aagagcaata
    21781 caactaatcc acgcaaataa gtataaaaca tgcgatctcc ttcgattgtt ttcttgttat
    21841 tattatacct tatcaaagga gggctggcaa acttttccct tgactagata catatttagg
    21901 atgaaattag aattctgtta aaaaaaatga tataatagaa tttatggata aaaataagat
    21961 tatgggatta acccaaagag aagtcaagga aagacaggct gagggtttgg tcaatgactt
    22021 taccgcatca gccagtacca gcacttggca aatcgttaaa cgaaatgtct ttaccctttt
    22081 taacgctttg aacttagcca ttgctttggc tcttgccttt gtgcaggctt ggagcaatct
    22141 ggtcttcttt gctgttatct gctttaacgc tttttctggg attgtgaccg agctacgagc
    22201 caaacacatg gtggacaagc tcaatctcat gaccaaggaa aaggtcaaaa ccatccgtga
    22261 tggtcaggaa gttgctctta atcctgaaga attagtgcta ggagatgtca ttcgtttgtc
    22321 tgcaggagag cagattccta gtgatgcctt ggttttggaa ggctttgcgg aagtcaatga
    22381 agccatgtta acgggagaaa gtgatttggt gcaaaaggaa gttgacggct tacttttgtc
    22441 aggaagtttc ctagccagtg ggtcagtttt atctcaagtt caccatgtcg gtgcagacaa
    22501 ctatgctgcc aaactcatgc ttgaggctaa gaccgttaaa cccatcaact cccgtatcat
    22561 gaaatcgctg gacaagttgg ctggttttac tgggaagatt atcattccct ttggtctggc
    22621 tctcttgctg gaagccttgc ttttaaaagg cctgcctctc aagtcatctg ttgtaaactc
    22681 gtcgacagct cttttgggaa tgttgcctaa gggaattgcc cttttgacca ttacttcgct
    22741 cttgactgca gttatcaagc taggtttgaa aaaggtcttg gtgcaggaga tgtactctgt
    22801 tgagaccttg gcgcgcgtgg atatgctctg tctggacaag acgggtacca tcacccaagg
    22861 aaagatgcag gtggaggctg ttcttccgtt gacggaaacg tatggtgaag aggctattgc
    22921 cagcatcttg actagctaca tggcccatag tgaggataag aatccaactg cccaagccat
    22981 tcgccagcgt tttgtgggag atgttgctta tcctatgatt tccaatcttc ccttctcgag
    23041 cgaccgcaag tggggggcta tggagttaga aggcttgggg acagttttct taggggcacc
    23101 tgagatgttg ctggattctg aagtcccaga agccagggag gccttggaga gaggatcacg
    23161 tgtcttggtc ttagctctca gtcaggagaa attagaccat cacaaaccac agaaaccatc
    23221 tgatattcag gctctagcct tgctggaaat cttggacccc attcgagagg gagcagcaga
    23281 gacgctggac tatctccgtt ctcaggaggt aggactcaag attatctctg gtgacaatcc
    23341 agttacggtg tccagcattg cccagaaggc tggttttgcg gactatcaca actatgtaga
    23401 ttgctcaaaa atcaccgatg aggaattgat ggccatggcg gaggagacag ctattttcgg
    23461 acgtgtttcc cctcatcaaa agaaactcat catccaaacg ttgaaaaaag cgggtcatac
    23521 aacggctatg acaggggacg gggttaatga tatcttggcc cttcgtgagg cggattgttc
    23581 tatcgtgatg gcggaggggg atccagcaac ccgtcagatt gccaatctgg ttctcttaaa
    23641 ctcagacttt aatgatgttc ctgagattct cttcgagggt cgtcgcgtgg tcaataacat
    23701 tgcccacatc gccccgattt tcttgataaa gaccatctat tccttcctgt tagcagtcat
    23761 ctgtattgcc agtgctttac taggtcggtc agagtggatt ttgattttcc ccttcattcc
    23821 gatccagatt accatgattg accagtttgt ggaaggtttc ccaccattcg ttttgacttt
    23881 tgagcgaaat atcaaacctg tcgagccaaa cttcctcaga agatccatgc ttcgtgccct
    23941 accaagtgcc ctcatggtcg tgtttagcgt tctttttgtg aaaatctttg gaacgagtca
    24001 aggttggtct gagttagaaa tctcaactct cctctattat ctcttggggt caattggttt
    24061 cttatccgta tttagagcct gcatgccatt cactctatgg cgagtcctct tgattgtctg
    24121 gtcagtagga ggcttcctag ccacagctct cttcccaaga atccaaaaac tgcttgaaat
    24181 ttcaaccttg acagaacaaa cgttgcctgt ttatggtgtc atgatgttgg tctttaccgt
    24241 gattttcatc ctgactagtc gctatcaagc tagaaaataa agaaagactg caatctgtgg
    24301 attgcagttt ttttaggtac aagattgcca gccgaaatgt ggtataataa gaggtaatag
    24361 agtttggaaa gtgagagaaa atgatttcaa agagattaga attggtagct tcctttgtgt
    24421 cacagggggc tattttacta gatgtgggaa gtgaccatgc ttatctgcct atcgagttgg
    24481 ttgagagagg ccaaatcaaa agcgctattg caggtgaggt ggtggaaggt ccctatcagt
    24541 ctgcggttaa aaatgttgag gctcacggcc taaaggagaa aatccaagtc cgtttagcca
    24601 atggcttggc agcttttgaa gagactgacc aagtgtctgt cattaccatt gctggcatgg
    24661 gtggtcgttt gattgctagg attttagaag aaggtttggg gaagttagct aatgtagagc
    24721 gtttgatcct ccagcccaat aatcgtgaag acgacttgcg tatctggcta caggatcatg
    24781 gattccagat tgtagcagaa agcatcttag aagaagctgg aaagttttat gagattttgg
    24841 tggtggaagc aggacaaatg aagctatcag ccagtgatgt tcgctttggt cccttcttgt
    24901 ccaaagaagt cagtccagta tttgtccaaa aatggcaaaa agaagctgag aagctagagt
    24961 tcgccctcgg acaaatccca gaaaaaaatc tggaagaacg tcaagttcta gtagataaga
    25021 ttcaagctat caaggaggtg ccccatgtta gcaagtgaag tgatccaagc ttatgaagcc
    25081 ttttgccctc aggaattttc tatggaggga gacagccgtg gtctgcaaat tggcacttta
    25141 gacaagggta tccaaagggt catggttgcc ctcgatattc gtgaagagac agtggcagag
    25201 gccattgaaa agggtgtgga cttgattatc gtcaagcacg cgcctatctt tcgtcctatc
    25261 aaggacttgc ttgccagccg tccgcaaaat cagatttaca tcgacctgat taagcatgat
    25321 atcgcagttt atgtcagcca taccaatatt gatatcgttg aaaatgggct caatgactgg
    25381 ttttgtcaga tgctaggaat cgaggagacg acttatctgc aggaaacagg tccagaacgt
    25441 ggaattggac gtattgggaa tattcagcct cagacatttt gggaattggc ccaacaggtc
    25501 aagcaagtct ttgacctaga tagccttcga atggtgcatt atcaagagaa tgatttgcag
    25561 aagcctattt caagagtagc aatttgtggt ggaagcgggc agtctttcta taaggatgct
    25621 ttggcaaagg gggcagatgt ctatatcact ggcgacatct actaccatac tgctcaggat
    25681 atgctgtctg atggcttgtt agcattggat ccaggtcact atatcgaagt gctttttgtg
    25741 gaaaaaatcg cagcactcct tactcaatgg aaagagaaaa aaggctggga actagagatt
    25801 ttacctagtc aagcatcgac caatcctttc cgtcatatct agttagaaag tgaagacaat
    25861 gaaaaaagtt gccattattg gagcaggaat tgtgggagca acagctgcct actacctctc
    25921 gagagaaagt gacctagagg tgaccgtttt tgaccatgag caaggtcaag ccaccaaggc
    25981 cgcagcagga attatcagtc cttggttttc caaacgccgt aataaagcct ggtataagat
    26041 ggcgcgcttg ggggctgatt tttatgtgga tttattagct gatttagaga aatcaggaca
    26101 agaaatcgac ttttaccagc gttcgggagt ctttctcttg aaaaaggatg aatccaagtt
    26161 ggaagaactc tatcaactgg ctctccagcg cagagaagaa tctcccttga taggccagtt
    26221 agccattctg gaccaagccc cagctaatga attattccct ggcttgcagg gattcgacaa
    26281 cttgctctat gcttctggtg gagcgagagt agatggtcac ctcttagtaa ctcgtttgct
    26341 ggaagccagt caggtcaagt tggtcaaaga aaaagtgact ctgacactct tatcatcagg
    26401 ctaccagatt ggcgaagagg tgtttgatca ggttattttg gcaacgggag cttggttggg
    26461 ggacatgtta gagcctttag gttatgaagt ggatgtccgt cctcaaaaag gacaactacg
    26521 agattatcag cttgtccaag acatggaaga ttaccctgtt gtcatgccag aaggggagtg
    26581 ggatttgatt ccctttgcag gtgggaaatt atccttgggc gctacccatg aaaatgacat
    26641 gggatttgat ttgacggtag atgaaccctt gctccaacaa atggaggagg cggccttgcc
    26701 tcattaccca gttttagcta gagtgacttc aagggctgag cgtgtgggaa tccgtgccta
    26761 taccagtgat ttctctccct tctttgggca ggtgccagaa ttggcaggtg tctatgcagc
    26821 cagtggacta ggttcatcag gcctcacaac tggtcctatc attggttacc atctagccca
    26881 actgatccaa gacaaggagt tgaccttgga ccctctaaat tacccaattg aaaactatgt
    26941 caaacgagta aaaagcgaat aatactcaat gaaaatcaaa gaacaaacta ggaaactagc
    27001 cgcgggttac tcaaagcact gctttgaggt tgtggataga actgacgaag tcagctcaaa
    27061 acactgtttt gaggttgtgg atagaactga cgaagtcagt aaccataccc atggcaaggc
    27121 gacgttgacg tggtttgaat tggattttcg aagagtataa gcattttact gaaattttat
    27181 caggtagttt aggatggcaa atgacattcc ctgtcaaaaa tggtaaaata agaaaaaatg
    27241 atctgagaat cgaggaaaaa agatgcaaga aaagattttg gtaactggtg gtgccggttt
    27301 tattggaacc cacactgtta ttgaattgat ccaagcaggc catcaagttg ttgtggtgga
    27361 taatcttgtc aatagcaatc gtaagagttt agaagttgtt gaaagaatca caggagttga
    27421 gattcctttc tatgaggcag atattcgtga cacagatacc ctcagagata ttttcaagca
    27481 ggaagaaccg actggtgtca ttcactttgc tggtttgaag gctgttggcg aatcaacacg
    27541 tatccctctt gcctactatg acaacaatat cgctggaact gtcagccttt tgaaagccat
    27601 ggaagaaaac aactgtaaaa acatcatctt cagttcttct gcgacagttt acggggatcc
    27661 gcacacagtg cccatcttgg aagatttccc actttcagtg acaaacccat acggtcgtac
    27721 caagctcatg ctagaggaaa ttttgaccga tatttacaaa gcagactcag aatggaatgt
    27781 tgtcttgctt cgttacttta acccaatcgg agcccatgag agtggtgatt tgggagaaaa
    27841 tccaaacggt attccaaaca atctcttgcc atatgtgact caagtagccg ttggaaaatt
    27901 agagcaagtg caagtgtttg gagacgatta cgatacggaa gatggaacag gtgttcgtga
    27961 ctatatccac gttgtcgatt tggctaaggg tcacgttgca gctttgaaaa aaatccaaaa
    28021 aggttcagga ctaaacgttt ataaccttgg aactggtaaa ggttactcag ttcttgaaat
    28081 tatccaaaac atggaaaaag cggtgggacg tcctattcct taccgcatcg tagaacgtcg
    28141 cccaggtgat atcgctgcct gctactcaga cccagcaaaa gctaaagcag aactcggttg
    28201 ggaagcagaa ctcgacatca cccaaatgtg tgaagacgca tggcgttggc aaagcaagca
    28261 tccaaatgga tttgaagact aagatgatga tttcaatcat cgtcccttgt ttaaacgaag
    28321 aggaagtact tcctcttttt tatcaggctc tggaagcttt acttccagat ttggaaacag
    28381 aaatcgagta tgtctttgtc gatgatggat caagtgatgg gaccttggaa ctcttaaagg
    28441 cctatcggga gcaaaatccg gcagtccatt atatttcttt ctctcgaaat tttggcaaag
    28501 aagcagccct ttatgcaggc ttgcaatatg cgacaggaga tttggtggtg gtgatggatg
    28561 cagacctcca agatcctcct agtatgttgt ttgagatgaa aaatgtacta gacaaaaatg
    28621 tagacttgga ctgcgttggg acacggagaa ctagtcggga gggagaaccc ttctttcgca
    28681 gtttctctgc tgttctcttt tatcgcctca tgcaaaaaat cagcccagta gctctgccgt
    28741 cgggtgtccg tgattttcgt atgatgagaa ggtctgtagt cgatgccatt ttaagcttga
    28801 ccgagtccaa tcgtttttca aaggggctct tcgcctgggt gggtttcaaa acctactatt
    28861 tggactatcc aaatgtcgaa aggcaggctg gcaagacaag ttggagtttt aggcaactct
    28921 ttttctactc cattgaaggg attattaatt tttcagattt tcccttgatt atagcctttg
    28981 tagctggtct cctatcttgt tttctttctc tgctgatgac cttttttgtt gtggttcgga
    29041 ccctcatttt gggcaatccg acatctggtt ggacctctct gatggctgtt attctctttc
    29101 ttggaggcat tcaactcttg accattggga ttctcggcaa gtatatcagt aagatttatt
    29161 tagagactaa aaaaagacca ctttatctta tcaaagaaaa aagtgacctt cctgatttta
    29221 cagaaaaaaa taaagtgaaa agactataat tttacatgga aatgtgctaa actagaggga
    29281 gtggattatc gccattgatt taggcttcct ggtttccaat cagggcgcga gttgggcaat
    29341 ttttaacagc ctctaagaca tcctgactag gagaaatttc tttttccagt tggtcagggt
    29401 catcgtaaaa acgcacgatt ccattatcgt ggtaatcaaa taaatcagaa taagtttggc
    29461 aaagcccaca ggcgatacat cgttcaggta taagtgtgat tttcatgttc atatttatat
    29521 tgtaataaga aaagtaaaaa aaaacaagga gtaaggtatg gcaaaagaac cgtggcaaga
    29581 agatatctat gatcaagaag aatcaagagc agagcgtcgg catcgaaacc acggaggggc
    29641 ggataggatg gctaatcgta ttttgacgat cctagctagt attttctttg taattgtggt
    29701 ggtgatggtc atcgttctca tctatctatc atcggggggg agtaatcgca cagcagcctt
    29761 aaaagacttt catgattctg atgcaagtgt agtacaaatc tcatcttcaa gcagttctca
    29821 gcctgagcag agttcagagc cagaatctac ttctagtagt tcagaagaag ctgctaatcc
    29881 tgaaggaacg attaaagttc tcgcaggaga aggggaagca gctattgccg ctcgtgcagg
    29941 aatctccatt gctcagttag aggccttgaa tcctgggcac atggctacag gatcttggtt
    30001 tgctaatcca ggtgatgtta taaaaataaa ataggagccg atatgaaaac aattcaaatt
    30061 gctattgatg gtcctgcttc cagcggtaag agtacggtcg caaagattat tgctaaggat
    30121 tttggattca cctaccttga tacaggagct atgtatcgtg cagcgaccta tatggctctt
    30181 aagaaccaat taggagttga agaagtcgaa gcccttctag ccttgttgga ccagcatcca
    30241 atcagctttg gacgttcaga aactggagac cagcttgttt ttgtaggaga tgtggatatt
    30301 acccatccta tccgtgaaaa tgaagtgacc aatcatgttt ctgctattgc agcaattcct
    30361 gaagtgcgtg agaaactggt ttctctccaa caagaaattg cccagcaagg cgggattgtt
    30421 atggatggtc gcgatattgg aactgttgta ttgccgcaag cagaattgaa aattttccta
    30481 gtagcttctg ttgatgagag agcagagcgt cgttacaagg aaaatattgc caagggaatt
    30541 gaaacagacc ttgaaaccct aaaaaaggaa attgctgcgc gtgactacaa ggatagtcat
    30601 cgtgagactt ctcctctcaa acaagcagag gatgctgtct accttgatac aactggtttg
    30661 aacattcaag aagtagttga aaaaatcaaa gcagaagctg aaaaaagaat gtaaatgtag
    30721 aaaagccgat agattgatat cggttttttc taaatttgtc aaaatgctga ttttagggta
    30781 taatagttgc tgttcgagaa attttgattt tcaaagaata gtgaatgatg ataaggagaa
    30841 atcatgaaca atttaccaaa ttgtccaaaa tgtaactcag agtatgtcta cgaagacggt
    30901 gccctactgg tttgcccaga gtgtgctcat gagtggaatc ctgctgaagt tgcagaagta
    30961 gaagaaggtc ttgtcgctat cgatgccaac ggaaataaat tgactgatgg tgatacagta
    31021 actctcatca aggacttgaa agtaaaaggt gcgccaaaag atttgaaaca agggacgcgc
    31081 gtgaaaaata tccgcatcgt agaaggcgat cacaatatcg actgtaagat tgatggtttt
    31141 ggtgccatga aacttaaatc agagtttgtg aggaagattt aagcatgtca aaacactata
    31201 aacttgtatt ttatagccgt atcttcttgt ttctagcggc ttttacggga gtttatcttg
    31261 aaattactag gcatggtggt tttgggatgc ttctctatta cacggttctg tccaacctct
    31321 tggtaactat ttttaccctt tatcttctaa aggttatgag ccgtgtaggt gaaaattggc
    31381 aaaggccaag tctcttgcgc ttaaaaggtg gggtcaccat gagtatcatg attacctgtg
    31441 tgatttacca tttcctcttg gcgcccattg cgactaattt ctatacccta gaaaatttcc
    31501 tttgccacta tatcgttccc atctggtttt tagcggatac cctctttttt gacaaacagg
    31561 gtcaatacaa gatttgggat ccagcagtgt ggaccatttt accctttctg tatatgatgt
    31621 ttgctctttt taatggcttg gttctaaaac tcaatattcc aaatgccaag gataatcctt
    31681 tcccttactt ctttttgaat gtgaacaagg gttggaatgt tgtgtttaag tggtgtctga
    31741 ttatctttgt tgcctatatg gtagcaggat ttattttcta ctttatcaag caaatcaaga
    31801 gaaagtcatc ctaatactct tcgaaaatct cttcaaacca cgttagtttc catctgcaac
    31861 ctcaaaacac tgttttgaac aacctgcggc tagctttcta gtttgctctt tgattttcat
    31921 tgagtatgag atacaaggga ttcattttcg aatttaagaa ggagtctaca aaccaatgag
    31981 gcttcttctt ttcttgctta tttgataact atcaaaaaag tagaagatta agagaaatat
    32041 agaaagagat ttcatgaaac aatttttaga acgggccagc attttggctc tctccctcgt
    32101 tttgattacc tccttttcca tttcgagtgc cctaccagcc atgtttgact attatcaggg
    32161 ttattctaag gaacaaattg agctcttggt gagcttgcct tcctttggaa tcatgatgat
    32221 gttactgcta aatggtttct tagaaaaaat atttcctgag cgcttacaga ttagtttggg
    32281 cttgctgatt ttatcattga gcggtacagc tcccttctgg taccaagcct atccctttgt
    32341 ctttggaaca cggcttctct ttggtttggg tcttgggatg atcaatgcca aggccatttc
    32401 tattatcagt gaacgctacc aaggaaaaag gcgaattcag atgttagggc tacgcgcttc
    32461 tgcagaggtc gttggagctt ctctcattac cttggccgtc ggtcagttgt tggcctttgg
    32521 ttggacagct atctttctag cctatagtgc tggatttttg gtgatgcccc tttatctgct
    32581 ctttgtccct tatggaaaat caaagaaaga agtcaagaaa agagcgaagg aagcaagtcg
    32641 tttaactcga gaaatgaaag gcttgatttt taccttagct atcgaagcgg cagttgtagt
    32701 ttgtaccaat acagctatta ccatccgtat tccaagtttg atggtggaaa gaggattggg
    32761 ggatgcccag ttatctagtt ttgttcttag tatcatgcag ttgatcggga ttgtggctgg
    32821 ggtgagtttt tctttcttga tttctatctt taaagagaaa ctgctcctct ggtctggtat
    32881 tacctttggc ttggggcaaa tcgtgattgc cttgtcttca tccttgtggg tggtagtagc
    32941 aggaagtgtt ctggctggat ttgcctatag tgtagtcttg acgacggtct ttcaacttgt
    33001 ctctgaacga attccagcta aactcctcaa tcaagcaact tcatttgctg tattaggctg
    33061 tagtttcgga gcctttacga ccccatttgt tctaggtgca attggcttac taactcacaa
    33121 tgggatgttg gtctttagta tcttaggagg ttggttgatt gtaatctcta tctttgtcat
    33181 gtacctactt cagaagagag cttaggattg attcctaagt ttttcttttg atactcaatg
    33241 aaaatcaaag agcatagttg attgagtttg gaatagtacg ctgtagctgc taaaatattt
    33301 ctagaaatta atttgacttt tctaatagag ttattcatat cttatttcaa tctactatag
    33361 aaagctagcc gcaggttgct caaaatagtg ttttgaggtt gcagatggaa actgacgtgg
    33421 tttgaagaga ttttcgaaga gtatgagatt tttataccca tacaatattt gttttaggtc
    33481 cttgtttaca gactaatttc ttgataaaat agaggttatg acgagatata aagcaactat
    33541 ttcctatgat ggttatgcct ttgctggctt tcagcgccag tctcatgcgc gtagcgttca
    33601 ggaagaaatt gaaaaaacct tgaccagatt aaataaaggg caaaccatta ctgttcacgg
    33661 tgctggtagg acagatagtg gggttcatgc cctgggacag gtcattcatt ttgacctgcc
    33721 ttatcagatg gatgaggaga aactccgttt tgccttggat acccagtctc ctgaagatat
    33781 tgatgtgatt tcgattgagc ttgtggcaga tgattttcat tgccgttatg ccaaacatag
    33841 caagacctat gagtttattg tggatagagg acgtcccaaa aatcctatgc gccgtcacta
    33901 tgccacccac tttccctacc cactcgatgt ggaacgaatg cagattgcaa tcaaaaagct
    33961 agagggaacc catgatttta ccggttttac agcctctggg actagtgtag aggataaggt
    34021 tcgcaccatc acagaagcta gtttaatagt cgatgagaca ggacaatttt tgacctttac
    34081 cttttcagga aatggtttct tgtataaaca gattcgcaat atggtgggga ctcttctcaa
    34141 aatcggtaac aaccgcatgc cagtagagca gattgatctc atcttggaga agaaggacag
    34201 gcaacttgca ggtcccactg cagcaccaaa tggtttgtat ttaaaggaga ttcgttatga
    34261 agaataatcg tattttagca ctttctggaa atgatatttt tagtggtggt ggactgtcag
    34321 ctgatttggc tacctatacc ttgaacggct tgcatgggtt tgtagcagtg acttgtttga
    34381 cagccttgac agaaaaagga tttgaagtct ttccaactga tgataccatt tttcaacatg
    34441 aattagatag cttacgtgat gtggaatttg ggggaattaa gattggtctt ctccctactg
    34501 tcagtgtggc tgagaaggcc ttggacttta tcaaacaacg cccaggagta cctgtggtgt
    34561 tggatcctgt cttggtctgc aaggaaacgc atgatgtagc tgtcagtgag ctctgccaag
    34621 agttgattcg ctttttccct tatgtcagtg tgattacgcc taatctccca gaagcagaat
    34681 tattatccgg tcaggaaatt aaaaccttgg aagacatgaa aactgcagcg cagaaattgc
    34741 atgatttagg agcgccagca gtcattatca agggaggcaa tcgtcttagt caggacaagg
    34801 ctgtggatgt cttttatgat ggacagacct ttactatcct agaaaatcca gttatccaag
    34861 gccaaaatgc tggtgcaggt tgtacctttg cctctagcat tgccagtcac ttgattaaag
    34921 gtgataaact tttgccagca gtagaaagct ctaaggcttt cgtttatcgt gctattgcac
    34981 aagcagatca gtatggagta agacaatatg aagcaaacaa aaacaactaa aatcgccctt
    35041 gtatccctat taaccgccct ttctgtggtt ctaggttatt tcttaaaaat cccaacacct
    35101 acaggaattc taactctttt agatgctggt gtcttctttg cggcctttta ctttggtagt
    35161 cgtgaaggag cggtagtcgg aggactagca agtttcttga ttgacctctt atcaggctac
    35221 cctcagtgga tgttctttag cttggtcaac catggcttgc agggattttt cgcaggattt
    35281 aaaggaaaaa gtcagtggtt aggccttatt ttagcaacta ttgccatggt aggaggctac
    35341 gccttgggtt ctactttgat gaatggctgg gcagcagccc tcccagaaat tctaccaaat
    35401 ttcatgcaaa atatggtagg gatgattgta ggatttattc ttagtcaaag tatcaagaag
    35461 attaagtaat actcttcgaa aatctcttca aaccacgtca gcttccatct gcaacctcaa
    35521 aacactgttt tgagcagcct gaggctagct ttctagtttg cactttgatt ttcattgagt
    35581 ataaagaggt tgagtaaaaa gtatttcaaa taagaaaaac gcatagtatc aggtgttgaa
    35641 tgaactgccc cccaaaagtt agacagaaaa aatctaactt ttggggtgtt ttattatgaa
    35701 actaacttat gatgataaag ttcagatcta tgaacttaga aaacaaggat atagcttaga
    35761 gaagctttca aataaatttg ggataaacaa ttctaatctt aggtacatga ttaaattgat
    35821 tgatcgttac ggaatagagt tcgtcaaaaa agggaaaaat cgttactatt ctcctgattt
    35881 aaaacaagaa atgattcata aagtctgaca tgaaggctag actaaagata gagtttctct
    35941 tgaatacggt ctcccaagtc gtacgatact tcttaactgg ctagcacaat acaggaaaaa
    36001 cgggtatact attgttgaga aaacaagagg gagagtacct aaaatgggac gtaagccaaa
    36061 aaagagacct gaagagagga cagaattaga acgtcttcaa gcagaaaatg agtacctgag
    36121 agcggagaat gccatcctaa aaaagttaag agaactccga ttgaaggagg aaaaagagaa
    36181 agacagaaat tgtttaagaa ttaatgactg agttttcgtt agatcttctt ctaaaagcca
    36241 ttaaactagc tcgttcgacc tactactatc acttgaaaca gctagacaaa acagataagg
    36301 accaagagct taaagctgaa attcaatcca tttttatcga acacaaggga aattatgctt
    36361 atcgtcgaat tcatttagaa ctaagaaatc gtggttatct ggtaaatcat aaaagagttc
    36421 aaggcttgat gaaagtactc aatttacaag ctaaaatgcg acagaaacga aaatattctt
    36481 ctcataaagg agacgttggt aagaaagcag agaatctcat tcaaggccaa tttgaaggct
    36541 ctaaaacaat ggaacagtgc tacacagatg tgactgaatt tgccattcca gcaagtactc
    36601 aaaagcttta cttatcacca gttttagatg gctttaatag cgaaattatc gcctataatc
    36661 tttcaacttc acccaactta gaacaagtac aaacaatgtt ggaacaggca ttcacagaga
    36721 agcactacga gaatacgatt ctccatagtg accaaggctg gcaatatcaa cacgattctt
    36781 atcatcagtt tcttgagggt aagggaattc aagcatccat gtcacgcaag ggaaacagcc
    36841 aagacaacgg tatgatggag tccttctttg ggattctgaa atcggaaatg ttttacggtt
    36901 atgagaagtc gtttcagtcg cttaagcaat tggaacaagc tattgtagac tatattgatt
    36961 actacaacaa taaacgaatt aaggtaaaac taaaaggact tagtcctgtg caatacagaa
    37021 ctaaatcctt cggataaatt atttgtctaa ctgtttgggg gcagtacatt ttaacacttg
    37081 atactatgcg ttttattgtg ggaagatttt cctcattttc tagtgaaatt gaactttttc
    37141 taacatcttt tgatagatga cttcttaatc aattatcatt tccattgttg tttcttgggg
    37201 tttcatccct tgtctttgat aaaagcggag agcaccctcg ttatcattcc agacacttag
    37261 ggttagatta ttgcagccaa tttctttagc ataatagcag aaaaatgaca ataaaatagc
    37321 ccctagatat tttgaaagga aagcataggg gctatatagt ttaatcaaaa taaagcaaat
    37381 ctttgctggt gcttgtaaag aggtcgaagc catccttggt aacaacaccg cagtcttcaa
    37441 tacgaacacc gactttacca gggatataga tacctggttc aacagagaag cacatgcctt
    37501 cttcgatgac catgtcgttt ccttccatga tagatgggaa ttcatggaca tccataccga
    37561 taccatgccc gagacggtgg ttgaagtact caccataacc agctttttcg atgacctcac
    37621 gggcagcgcg gtccacttca tgagcagtca cacctggctt gataaagtca agagcagctt
    37681 gttgggcttc aagagtcaag ttgtaaatat ctttcttgaa ttggtctggt ttgccgacag
    37741 cgactgtacg agtcatatct gacgcatagc cattgaccag aacacccagg tcaaagagga
    37801 gaagagcatc attttcaacc ttattagctg ctggaatgcc gtgtggattc gcagcattat
    37861 caccagtcaa gaccatggta tcaaagctca tttcataacc ttcacgtttc atggcaaagt
    37921 cgatttgtgc gatgatatct gtctcagtct tatcaagaga aatattgtca aaaccaacat
    37981 gaacagcctt gtcagcataa agacctgcaa ccatcatttt ttgcacttca tcagctgatt
    38041 tgatgaggcg catgcgttgg atacgaggag tgaggttgtc aaactcagca gtctcaaaaa
    38101 ctgttttcaa accatggtat ttggtcaaga tgagattgtc aaactcaaca gcgacacgtt
    38161 tgaagtcaag ttgtggaaga gcatgtttga ttttttgcca tggattttca gaatcgacat
    38221 agcccactac tgggaaggaa acggtgctac ttgcacgttc tacttcaaga gctgggacaa
    38281 agaggagagg ttcctgatcc gctaggacaa agaggaacat ttggcgttca tggggatcac
    38341 tgtaaaaacc agtgaggtaa ttgattgtga cggggtcaga tacgacagcg acgtctagtt
    38401 tttctgattc aagatatgtt aggatttgtt gtaatttaga catatgctac cttctttcta
    38461 cccctctatt ttggcaaaaa atgagagaaa atgcaaggga gaagggtaaa agaaaagaca
    38521 aaaaactccg acaagataac ggagttcttt gatatcattt tcctttgttt aagaaaagtc
    38581 agccttgctt ttgacgtcac tgtactcttc agcgatttct tggctgagaa tgtcctcata
    38641 ggcagggagt tggatgtcct gaccatattt cttcttgagg gcagtagtaa ctaggcgata
    38701 agccagattg gcattacgag agaggatagg cccgtggaag taggaaccaa agacattctt
    38761 ataatgaacc ccttcaccga ccttttcttc gttgtttcca tttccataga caacctgtcc
    38821 cagcggtttt tggtcatcag agaggaaggt acgaccttgg tgattttcaa atccatagta
    38881 ggtttcatcg aaatcttcat tgtgaatctt gatgtcaccg ataaaacggt tattggtctg
    38941 gttgagcgtg tagtgtccca tgacccctag cccttcgata cgttttcctg aagcttcaac
    39001 ataatattga cccaatagtt ggaaaccacc gcagatagcc agaactacac cgtcgttttg
    39061 gatgtagttg tcaatgctct cttttttagc aggtaggtcg tctgcaatga tactttgttc
    39121 aaagtcttga ccaccaccga aaaaggcgat gtcgtagtga ttttcatcaa agtcatcatg
    39181 gagagaaacg atgtcaacgg tcacatgggc tcccagtttt tcagccacat acttgagcat
    39241 gaggatgttt ccattgtccc cgtaggtatt catgagattt ccgtagaggt gggcaatgtt
    39301 gagctgatag gggtaattgc tatcttttga ggaaagtgaa gtataaacca ttagttcatc
    39361 tcctttctaa caatctgacg actagccagc agttcacgaa attccagcat ggcagtataa
    39421 gttgccagaa tataggcatg cttgcagtct tgattctcaa tggtcttgag aacttgctcc
    39481 agattactcg tttcagtgat tttctcagct ggatagccag tcactcggag gcgacgagcg
    39541 atttcagaat gacgaacacc gccagcgttg atttcaggaa tgtccatgtc agtgatttgc
    39601 tcaaagtctg catcccagat ccagctagtg tcaattccat ctgcatagtt ggcattaagg
    39661 aggacagata ggctaaatgg ataaggtgct agtttgatca tttcgatagc ttgggttgca
    39721 ccgactggat ttttaatcaa gacaagggta cattccttgt caccgatatg aaaggtttct
    39781 tggcgtccaa agacagcacg gctcttgtca aatccctgtt tgatgagttg ggaatctgcg
    39841 ccgaggaaac gggcgatggc cacagcagct agggcgttat agatattata gagcccgccg
    39901 atttggatac cgtattcttg gccgtctatg acaaagcgag agcgattgtt ggtcaactca
    39961 accagttttg tcaaacgata gtcgagatca ggacgtttac atccacaacc ttcacagata
    40021 taggcaccca agtttgcata ggtattatgc tcatatttga ggatgccttg gcagtcagga
    40081 cagagaatcc cttcggtatt gtagtgagca agttgggctg gtcctttttc caagtcaaaa
    40141 ccaaaatact ctatagggtt tggaatagtt ggcttgtaga aaagtggact gtctccgtta
    40201 aggagaacag tagcagttgg aacttttcga atggcatcca atatcatgtt ataggtagta
    40261 tagatttcac cgaaacggtc catctggtca cggaagatat tagtaatgac aaaaagacta
    40321 ggctggatat agtcacagat acgagataga ctggcttcgt caatttcgag gacggcaata
    40381 tttttcccag tttttgaaga tttggctgtt aggaaggttg ttgcaatccc tgtaatcatg
    40441 ttggcaccgc ttgggttggt tagaacttga ccataaacct cttttaaaat gccgacagtg
    40501 agggcagttg tcagggtttt tccatttgtt ccagtgacaa cgacaatctc gtagttctta
    40561 gctaggcttt gtaaaatatc tttatcaaat tgaagggcga ctttccctgg gagcgtactt
    40621 ccacgtccaa gacggcttaa aacgaagtgg gaagaacgcc cagcaagaag gcccaaagta
    40681 gtttttaagt tcatgtttct attatatcac aaaagaggga aaagacagat ttggtgtttc
    40741 gtagaagtaa aaacagccca aagagggctg tcagttaaga tgagatttca acttaaatcg
    40801 caaacaagtc attcaagttc tcagccaagg ttgggtgagt gaagatttgt tttgtgaagt
    40861 aagtgtaagg aatcttgttg tccatagcaa cagtaatgat gttgatgatt tcttgagaac
    40921 cttctgagaa gatgcttgca ccaagaattt cttttgtttc agtattaaca acagctttga
    40981 aagctccgcg aaggtctcca tttacgtgac cacgaggcat ggctgcaaca gggatttctt
    41041 tcactgcgta tggaagtttc aaatcagctg cttggctttc agtcaaacca acttgtgaaa
    41101 gtgcaggtgt gatgaacata gtatttggca cattgagacg gtcctcaagt gtgtagctgc
    41161 catctccagc aaggtagctg taaacaacac ggaagtcatc aagtgaaatg taagtaaatt
    41221 gaaggccacc gttgacatct ccaactgcaa agacaccagg aacgtttgtt tgacagtgtt
    41281 tgtctacttt aatagcacca cgttcagtta gttcaatatc tgtattttca agttgaagtg
    41341 gttctacatt tggtttgcgt ccagttgcgt agagaagggc gtcgaaacgg taagtttcgt
    41401 cttcagttac gacaagcact tggtcaccat cgtttttgat ttcagtagta tggatatttt
    41461 gaagcaattc aatgccatct tcttccatgt attgtttagc aagagctgcg atggaaggtt
    41521 ctgcacgagg taggaatgta tccaaggcat ctaggactgt gaccttgctt ccaagtttgt
    41581 tgtaaaggcc ggcaaattca agaccgatat ttccgccacc aaggattcca agtttttcag
    41641 gtaatttgtc caagctttgg atacctgttg agtcaaagat gtttttgctt gtagcaagtc
    41701 caggaattgg caagacgttt gaaacagcac cagtgttgat gacgattgtt tcagcagtca
    41761 gttctttctt ttcatcacca gcttggattt cgatgacttt atttgaaagg aagtgagctt
    41821 ccgcatcaaa gatatctacg cctgtaccag caacagtcgc atagttttta ccgttgaggc
    41881 gaccagtgat cgtgttttta gtagcaatca cttcttcaaa agacaagtcc ttttcagcag
    41941 caactagcaa ggttttagtt gggatacaac caatgttgat acaagttcca ccgtacatag
    42001 ccttgctgcg ttcaacgagg gcaacttttt tgccagctga agccaattta cctgctagtg
    42061 ttttaccagc tttaccaaat ccaataacaa ttaaatcgta tgttaacatt tatcttcctc
    42121 ctattcgaat ttcattctag cacaagaaaa aaacttttgc acaattctgc tacggaaaag
    42181 acagaaaaag ataaaaacgc tttccagaca aaaaactagt aattttttga caagcttgat
    42241 aaactaaaat cctatctatt tttatataag aaagaataaa tgttcgaaaa taggaggttt
    42301 tatgaaatcg aatcgttata ttattgcctt tgctggggtc attttacact taatgctggg
    42361 ttcgacttat gcttggagtg tttatcgtaa ccctattatt gaaaaaacgg gatgggatca
    42421 ggcttctgtt gccttcgcct ttagtctagc aatcttttgt ttgggcttat cggctgcatt
    42481 tatggggcgt ttggtagaaa aatttggtcc gaaagtcatg gggagtctat ctgcttttct
    42541 atacgcaggt ggaaatatct taacaggatt tgcaatagac cgtcaggaac tgtggttgtt
    42601 gtatctagct tatggcattt taggtgggct tggtttggga gcaggctata ttacccctgt
    42661 gtcaacgatt ataaaatggt ttcctgataa acgtggtctc gcaacaggtt tagcgattat
    42721 ggggtttggt tttgcttctt tattgactag tcccatagcg caacacctca tcgcaggggt
    42781 agggattgta gaaacttttt atattttagg agcaagttac tttattatca tgctcctagc
    42841 ttcacaattc attaagcgtc caaatgagca agagcttgca attttatctt cttcagggaa
    42901 agaaaaaaca gcctctttga cgcaaggaat ggctgcaaat caggctctaa aaagcaatcg
    42961 gttttatatg ctttggatta ttttctttat caacatagct tgtggtttag gcttaatttc
    43021 agcggcatcg ccaatggcac aggagatggc tggcttgtct acaagtcatg cagcagtaat
    43081 ggtgggtgtt ttggggattt tcaatggatt tggtcgcttg ctctgggcga gtttgtctga
    43141 ctatatcggt cgccctctaa cctttagtat attactgctt gtcaatcttt tcttttctct
    43201 ctcactttgg ctctttacag attccgtttt atttgtagtt gctatgtcta ttttgatgac
    43261 ttgctatgga gctggttttt ctttgattcc agcttatctc agtgatattt ttggaaccaa
    43321 ggaattggcc gctctgcatg gttatatttt aacagcttgg gcaatggctg gtttagcggg
    43381 acctatttta ttagcagaga cttataaaat ggctcattcg tacacacaaa ccttgttcgt
    43441 ttttctcatt ttatacagta tcgccttggc tttgtcttat tatctaggtc gttcaatcaa
    43501 aaaagaaagt caaaaaccgc ttacatgatt ttctgtagaa atggggctat cttttgctct
    43561 ggtcttgtgt tatactagat aggttgcaaa gaaaacagta cttttctttt gtggaaaaga
    43621 agcaacacga ttttatatct agttatatga aaatacgtca taaaaagaaa agtataaact
    43681 aagccctata gggtggcttc gcaccacctt tagaaagaag aataacgtga aatttaatga
    43741 attaaacttg tctgctgatt tgctagcaga aattgaaaaa gctggttttg tagaagctag
    43801 tcctatccaa gaacaaacta ttcctttggc ccttgaaggc aaggatgtta tcggtcaagc
    43861 tcagactggt acaggaaaaa ctgcagcctt tggcttgcct acccttgaaa aaatccgtat
    43921 agaagaagcg actattcaag ccttggtcat cgctccaact cgtgaactag ctgtccaaag
    43981 tcaagaagaa ctcttccgct ttggtcgtag taagggagtc aaagtccgtt cagtatatgg
    44041 cggatcaagc attgaaaaac aaattaaggc tcttaaatct ggtgcccata tcgtggtggg
    44101 aactccaggt cgcctcttgg acttgattaa acgcaaggcc ttgaaattac aagacattga
    44161 aacccttatc cttgacgaag cggatgaaat gcttaacatg ggcttccttg aagacatcga
    44221 agccattatt tcccgtgtac ctgagaaccg tcaaactttg cttttctcag caactatgcc
    44281 agatgccatc aaacgtatcg gtgttcagtt tatgaaagcc cctgaacatg tcaagattgc
    44341 ggctaaggaa ttgacaacag aattggttga ccagtactat atccgtgtta aggaacaaga
    44401 aaaatttgac accatgactc gtctcatgga tgtggcacaa ccagaactcg ctattgtatt
    44461 tggtcgtacc aaacgccgtg tggatgaatt gactcgtggt ttgaaaattc gtggcttccg
    44521 tgcagaagga attcatggcg acctagacca aaacaaacgt cttcgtgtcc ttcgtgactt
    44581 taaaaatggc aatcttgatg ttttggttgc gacagacgtt gcagcgcgtg gtttggatat
    44641 ttcaggtgtg acccatgtct acaactacga tattccacaa gatcctgaga gttatgttca
    44701 ccgtatcggt cgtacaggtc gtgctggtaa gtcaggtcaa tctattactt ttgttgctcc
    44761 aaacgaaatg ggttaccttc aaatcattga aaacttgact aagaaacgca tgaaaggtct
    44821 caaacctgca agtgtagaag aatccttcca atcaaaaaaa caggtagctc tcaagaaaat
    44881 cgaacgtgat tttgcagatg aaaccattcg tgccaacttt gagaaatttg gtaaggatgc
    44941 tcgcaaattg gctgctgagt ttactccaga agaattggca atgtatatct tgagtctgac
    45001 agtccaagac ccagatagcc ttccagaagt ggagattgca cgtgaaaaac cactaccgtt
    45061 taaaccatca ggtaatggtt tcggtggtaa agctaaggga ggtcgtggag gccgtcgtgg
    45121 ggacgaccgt cgagagcgtg atcgtcgtgg caatggtcgc cgtgatgagt tcaaaaaagg
    45181 aagtcgtggc aacgatcgtt ttgataagga aaaacgttac cgtaaggata ataaaaaacc
    45241 acgcaatact ttaagcgaaa agcaaacagg ctttgttatt cgtaacaaag gtgataaata
    45301 agaaaaagcg gttcaaaatg aatcgctttt ttatataagg agaccgaatg tgaaaaagac
    45361 aaataaaatt attatattat cttttataat attatgatac acaaaaatag gcgacttgtc
    45421 aatagaaaag gaacaattta attaaaaata ttctggtttt taattaaatt gtaagtcaat
    45481 gcaagtcaat aaggaatttt cagataatta ttgaaaaaga gacgttttta tgttataatg
    45541 atagcgatta aattcgaggt gaaaaatggc acatttatta gaaaaaacta gaaaaattac
    45601 atcaattttg aaacgctcag aggagcagtt gcaggatgag ctcccttaca acgctattac
    45661 gcgtcagtta gcggatatta ttcattgcaa tgcctgcatt atcaatagta agggacgtct
    45721 gcttggctat tttatgcgtt ataaaacaaa tacagatcgc gtagagcaat tcttccaaac
    45781 taagattttc ccagatgact acgttcaagg ggctaatatg atttacgaaa cagaagcaaa
    45841 cttgcctgtt gagcatgata tgagtatttt ccctgttgag agtagagatg attttccaga
    45901 tggcttgacg actattgcac cgattcatgt atcggggatt cgccttggtt ctttgattat
    45961 ttggcgtaat gataaaaaat tcgaagatga ggacttggtt cttgttgaga ttgccagtac
    46021 cgttgttggg attcagcttc ttaacttcca acgtgaagaa gatgagaaaa atattcgtcg
    46081 tcgtactgct gtcaccatgg cggtcaatac cctttcttac tccgaactcc gtgctgtttc
    46141 agcaatttta ggggaattaa atggaaatga agggaagttg actgcgtcag tgattgcaga
    46201 tcgtatcgga atcactcgct ctgtgattgt caatgctctt cgtaaacttg agtctgcggg
    46261 gattattgaa agtcgctcac ttggaatgaa gggaacctat cttaaggtct tgatttcaga
    46321 tatttttgaa gaagtgaaga aaagagatta ctaatgacaa aggctttaat ttcgattgat
    46381 tatacagaag attttgttgc tgatagtggg aagctgacag caggtgctcc agctcaggcg
    46441 atttcggatg ccatcagcaa ggtaactcga ttagcttttg aacgaggaga ttatatcttc
    46501 tttactattg atgctcatga agaaaacgat tgtttccatc cggaaagtaa gttatttcct
    46561 cctcataatc tgattgggac gagtggacgg aatttatatg gagatttggg gatcttttat
    46621 caagagcatg gttcagacag tcgtgtcttt tggatggata aacgccatta ctcagctttt
    46681 tcagggactg acctagatat tcgtttgaga gagcgtagag tgtctaccgt tatcttaaca
    46741 ggtgtcttga cggatatctg tgtcctacat acagctatag atgcctataa tctaggatat
    46801 gacatcgaga ttgttaaacc agctgttgct tccatctggc ctgaaaatca tcaatttgcc
    46861 ctaggtcatt tcaaaaatac actcggagct aagttagtag atgaaaatct aaatgaactt
    46921 tctgagtaac ctggttggtg aaaatgaaaa aatttgaaaa aaagtgttga caagcatccc
    46981 aaaagttgat atactagtaa agtaatcgat gcggggatgg cggaattggc agacgcgcag
    47041 gactaaggat cctgtgaccg ctttaggtcg tgagggttca agtccctctc tccgcatagg
    47101 aaaagagtta gctaaagcta gctcttttgt atttctctaa aaaattagaa tccagtcaaa
    47161 tgtagtggag aatcagcgaa aatatgggaa aactgatcct tgtttctgct tttgtcatag
    47221 aagaataata aagattatct tcttcaaatt ctccgatatt ctctaaagtt ttgtgcaagt
    47281 tgcacagaac ttttttattt ttttggtcat cttgccatag aaatataaag cgttttcatc
    47341 tataatataa ttatcaaaag acaaaaggag ttcacctcat ggtagaattg aatcttaaaa
    47401 atatttacaa aaaatatcca aacagcgaac actattcagt tgaagatttc aacttgaaca
    47461 tcaaagataa agaatttatc gttttcgtag gaccttcagg atgtggtaaa tcaactacac
    47521 tccgtatgat tgctggtctt gaagacatca cagaaggtac tgcatctatc gatggcgtgg
    47581 ttgtcaacga cgtagctcca aaagaccgtg atatcgccat ggtattccaa aactacgctc
    47641 tttacccaca catgactgtt tatgacaaca tggctttcgg tttgaaattg cgtaaataca
    47701 gcaaagaaga cattaacaaa cgtgttcaag aagcagctga aatacttgga ttgaaagaat
    47761 tcttggaacg taaaccggct gacctttcag gtggtcaacg tcaacgtgtt gccatggggc
    47821 gtgcgattgt ccgtgatgcg aaagtattct tgatggacga acctttgtca aacttggatg
    47881 ccaaacttcg tgtatcaatg cgtgctgaaa tcgctaaaat tcaccgtcgt atcggagcta
    47941 caactatcta tgtaactcac gaccaaacag aagcgatgac acttgcagac cgtatcgtta
    48001 ttatgtcagc tactaagaac cctgctggta caggtactat cggacgtgta gaacaaatcg
    48061 gtactcctca agaagtttac aaaaatccag ttaacaaatt cgttgcagga ttcatcggaa
    48121 gcccagctat gaacttcatc aacgtgaaat tggttggtag cgaaattgtt tctgacggtt
    48181 tccgtttgaa agtgccagaa ggagcattga aagttcttcg tgaaaaaggc tacgaaggaa
    48241 aagaattgat ctttggtatc cgtccagaag acgtgaatgc agaacctgct ttccttgaaa
    48301 cattcccaga ctgtgttgta aaagcgacta tctctgtatc agaactgctt ggttcagaat
    48361 ctcaccttta ctgccaagtt ggtaaagacg agtttgttgc aaaagttgat gctcgtgact
    48421 acttgcaaac aggtgcaaca gttgagcttg gatttgactt gaacaaagca cacttcttcg
    48481 atgtagaaac tgaaaaaaca atctactaag aataaataaa attcaaagca ctacaagaaa
    48541 agatatctct ttatcaattg tagtggagag atatcagtta atctagggag agaaacaaaa
    48601 tgcttctctc ctttttgcta gggaagtcat attatacatc tatattgtga tgctctttat
    48661 actcttcgaa aatctcttca aaccacgtca acgtcgcctt gccgtacgta tgattactga
    48721 cttcgtcagt tttatctgca acctcaaagc tgtactttga gtagcttacg gctagtttcc
    48781 tagtttgctc tttgatttcc attgagtatt atttgtgggt accatctaca agtgaagcta
    48841 tatgcgtaaa ctacgtgagc aattgaattc gaactagaga ggtaataata aatttatgct
    48901 atagttatgg tgacttgtat gcttttgatt ctagtttatc aaataataga ttagaattgt
    48961 cagataatat cattttgtgt cattttgtgt tataatgaag aaaaaacaga ggtgttcaaa
    49021 tgtcagaagc aggtcataag tttttagcaa aattggggaa aaaacgctta cgtccaggtg
    49081 gaaagcgtgc cacagattgg ttaattgcag aaggaggatt ttcaaaagaa aagagaatac
    49141 tagaggttgc gtgtaatagg ggaactacag caattgagtt ggcacagcgt tttggttgca
    49201 agataactgc tgttgatatg gatgctcaag ctttagaagt ggctaaaaaa tctgctggaa
    49261 cggcaggtgt tgctcattta atcagttttg aaagagcaaa tgcaatgaaa cttccttatc
    49321 aagatgctag ttttgatatt gttataaatg aagctatgct gactatgcaa gccgatcaag
    49381 ctaagaaaaa atgtgtaatg gaatatctaa gggtattaaa acctggaggt cttctcttga
    49441 cacatgatgt gcttcttaag gaagctaaag agtctatcag acaggaatta tcacaagcaa
    49501 ttcatgtaaa tgtaggtcct ttaactcaag atggttggga acaggtgatg atagaatcag
    49561 gttattgtga tgtgaaagta ttgactggtg aaatgacatt aatgaaatta tcgggtatga
    49621 tttatgacga aggttggcta ggaactttga aaatttgtgt aaatgcttgt aaaaaggaga
    49681 atagaaagca gtttttaact atgtataaaa tgtttgctaa gaataaacag aaattgggct
    49741 ttattgcgat ggctagttat aaatcgtcaa aacgttagat aattattgaa gttaactttt
    49801 ccttttttct ttcttaaaaa atatgctata atagagagta aaaaactttg aaagaaagaa
    49861 aaagatgaat ttaaaagatt acattgcaac aattgaaaat tatccaaagg aaggcattac
    49921 cttccgtgat attagtcctt tgatggctga tggaaatgct tatagctacg ctgttcgtga
    49981 aatcgttcag tatgctactg acaagaaagt cgacatgatc gtgggacctg aagctcgtgg
    50041 atttatcgtg ggttgtccag ttgcctttga gttgggaatt ggttttgcgc ctgttcgtaa
    50101 gccaggtaaa ttgccacgcg aagttatttc tgctgactat gaaaaagagt acggtgtcga
    50161 taccttgact atgcacgcgg atgccattaa gccaggtcaa cgtgttctta ttgtagatga
    50221 ccttttggcg acaggtggaa ctgttaaggc aactatcgag atgattgaaa aacttggtgg
    50281 tgttatggca ggttgtgcct tccttgttga attggatgaa ttgaacggcc gtgaaaaaat
    50341 tggtgactac gattacaaag ttcttatgca ttattaatga aaacagtccc tagggctgtt
    50401 ttctctacac taggatataa aaatagacta taactagtta gagaaaaact ataattgaaa
    50461 actatatctt cttacagtat aataaaagga ctaagtgttt gagatttgtc ttcaaacata
    50521 tgcaattatt cctgaaagag tacagttagg agagggttat gccgattcga attgataaaa
    50581 aattgccagc tgttgagatt ttacggacag agaatatctt tgtcatggat gatcaacgtg
    50641 ctgcccacca agatatccgt cctttgaaga ttttaatttt aaatctcatg ccacagaaaa
    50701 tggtcacaga gacccagttg ttgcgccact tggctaatac acccctacaa ctggatattg
    50761 attttctcta tatggagagc caccgttcta aaacaactcg ttcagagcac atggagacct
    50821 tctataaaac ttttcctgaa gtcaaggatg agtattttga tgggatgatc atcacgggtg
    50881 ctccagttga gcatttacca tttgaggaag tggactattg ggaggaattt agacagatgc
    50941 ttgagtggtc taagactcat gtctattcga cccttcatat ctgttggggg gctcaggctg
    51001 ggctttatct gcgctatggt gtagaaaaat accagatgga cagtaagcta tcaggtattt
    51061 atcctcagga caccctaaaa gagggtcacc ttctatttag aggctttgat gatagctatg
    51121 tatcccctca ttcacggcac acggagattt ctaaggaaga ggtcttaaac aagaccaatc
    51181 tcgagatttt atcagaagga cctcaggttg gggtttctat tttggccagt cgtgatttac
    51241 gagaaattta tagttttggt catttggagt atgaccgtga taccttggca aaagagtatt
    51301 ttcgagatcg tgatgcaggt ttcgacccac atattccaga aaattacttt aaagatgatg
    51361 atgttaatca ggtaccttgt ctttgttggt cttcatctgc agccctcttt ttcagtaatt
    51421 gggtaaacca tgcggtctat caggagacgc cttttgactg gagaaagata gaagatgatg
    51481 catctgcata taggtattta taagaggaat tatgacatat ttagacgctt ttaaatcagg
    51541 taccttggtt ttaccgagtg ccctgctctt gcattttaag gaactctttc cttctagcga
    51601 cgattttctg gtttggcaat ttttctattt gcaaaatacg acaggcttag aagaaatatc
    51661 gccaagccag attgctgaaa ggattggcaa ggaaatttcg gatgtcaacc agtccatttc
    51721 taatctgacg gaaaggggac tgctccagta tcgtactatc gaattaaatg gcgaaattga
    51781 attgctcttt gatgctagtt tggccttgga acgtttggat gacctgtttg gagcagttca
    51841 ttcaagttca gaccagctaa caccacaaaa ccagctcaag gatttggtgg aaaccttcca
    51901 gcaggagttg ggacgattgt tgacgccttt tgagattgag gatttgacca agacactaaa
    51961 ggaagatgga accagtgctg acttgattaa ggaggctctt cgtgaagctg ttttgaacgg
    52021 aaaaccaaac tggaagtaca ttcaggcgat tttgagaaac tggcgccatg aaggaatcaa
    52081 gagtgtggct caaattgagg ccaagagagc agaaagagaa gcaagcaatc ctcagttgac
    52141 acaggtatct gcagatttca taaatgccat ggatctctgg aaggattaat ccatgcaagt
    52201 aggcttgaaa tccgagtaag atttgcaagc tgtgtataat tgtgatagaa taaatagaaa
    52261 ataaattgaa aaaagaggta tgtgaaatgt cacgtaaacc atttatcgct ggtaactgga
    52321 aaatgaacaa aaatccagaa gaagctaaag cattcgttga agcagttgca tcaaaacttc
    52381 cttcatcaga tcttgttgaa gcaggtatcg ctgctccagc tcttgatttg acaactgttc
    52441 ttgctgttgc aaaaggctca aaccttaaag ttgctgctca aaactgctac tttgaaaatg
    52501 caggtgcttt cactggtgaa actagcccac aagttttgaa agaaatcggt actgactacg
    52561 ttgttatcgg tcactcagaa cgccgtgact acttccatga aactgatgaa gatatcaaca
    52621 aaaaagcaaa agcaatcttt gcgaacggta tgcttccaat catctgttgt ggcgaatcac
    52681 ttgaaactta cgaagctggt aaagctgctg aattcgtagg tgctcaagta tctgctgcat
    52741 tggctggatt gactgctgaa caagttgctg cctcagttat cgcttatgag ccaatctggg
    52801 ctatcggtac tggtaaatca gcttcacaag atgatgcaca aaaaatgtgt aaagttgttc
    52861 gtgacgttgt agctgctgac tttggtcaag aagtcgcaga caaagttcgt gttcaatacg
    52921 gtggttctgt taaacctgaa aatgttgctt catacatggc ttgcccagac gttgacggtg
    52981 cccttgtagg tggtgcgtca cttgaagctg aaagcttctt ggctttgctt gactttgtaa
    53041 aataatcagt aagtagcaaa agctaggtgg aacagcattc agatgtctgt tacatttttt
    53101 ataggagagg aaagattgaa aacaaaaatt ggattagcaa gtatctgttt actaggcttg
    53161 gcaactagtc atgtcgctgc aaatgaaact gaagtagcaa aaacttcgca ggatacaacg
    53221 acagcttcaa gtagttcaga gcaaaatcag tcttctaata aaacgcaaac gagcacagaa
    53281 gtacagacta atgctgctgc ccactggtat ggggattatt atgtaaagga tgatggctct
    53341 aaagctcaaa gtgaatggat ttttgacaac tactataagg cctggtttta tattaattca
    53401 gatggtcgct actcgcagaa tgaatggcat ggaaattact acctgaaatc aggtggatat
    53461 atggcccaaa acgagtggat ctatgacagt aattacaaga gttggtttta tctcaagtca
    53521 gatggggctt atgctcatca agaatggcaa ttgattggaa ataagtggta ctacttcaag
    53581 aagtggggtt acatggctaa aagccaatgg caaggaagtt atttcttgaa tggtcaagga
    53641 gctatgatgc aaaatgaatg gctctatgat ccagcctatt ctgcttattt ttatctaaaa
    53701 tccgatggaa cttatgctaa ccaagagtgg caaaaagtgg gcggcaaatg gtactatttc
    53761 aagaagtggg gctatatggc tcggaatgag tggcaaggca actactattt gactggaagt
    53821 ggtgctatgg cgactgacga agtgattatg gatggtgctc gctatatctt tgcggcctct
    53881 ggtgagctca aagaaaagaa agatttgaat gtcggctggg ttcacagaga tggtaaacgc
    53941 tatttcttta ataatagaga agaacaagtg ggaaccgaac atgctaagaa aatcattgat
    54001 attagtgagc acaatggtcg tatcaatgat tggaaaaagg ttattgatga gaacgaggtg
    54061 gatggtgtca ttgttcgtct aggttatagc ggtaaagaag acaaggaatt ggcgcataac
    54121 attaaggagt taaaccgtct gggaattcct tatggtgtct atctctatac ctatgctgaa
    54181 aatgagaccg atgctgagaa tgacgctaaa cagaccattg aacttataaa gaaatacaat
    54241 atgaacctgt cttaccctat ctattatgat gttgagaact gggaatatgt aaataagagc
    54301 aagagagctc caagtgatac agacacttgg gttaaaatca tcaacaagta catggacacg
    54361 atgaagcagg cgggttatca aaatgtgtat gtctatagct atcgtagttt attacagacg
    54421 cgtttaaaac acccagatat tttaaaacat gtaaactggg tagcggccta tacgaatgct
    54481 ttagaatggg aaaaccctta ttattcagga gaaaaaggtt ggcaatatac ctcttctgaa
    54541 tacatgaaag gaatccaagg gcgcgtagat gtcagcgttt ggtattaagc gatgatttga
    54601 aagagggatg tgatagtagc accctctttt tctttgtttt atgatagttc atcctcgagt
    54661 aaattcaaga tcttgcttgg aaatgaagct tatatagtag attgaatata gacaaatacc
    54721 ttgtgattgg taaaacattt tagaaattca tttacctttc ctaatcgact tggtttcatc
    54781 ttatttcaat ctattatagt attggggaat ttcttcaaac cacatcagct tggtcagttc
    54841 tacctgcgac ctcaaaactt gtgctttggt caaactgggt ttagtttcct agtttgctga
    54901 tggatttcca ttgactataa gcatccaacc ctctttttgt cttctaaaga attcttaaat
    54961 tatcagtcta ttgcaacttt tctcatataa gttctttgtc ttgctattgg ttttccttag
    55021 tagtatacta aggtagtaat cattaagaag tggttacaaa aaataatgaa tgaggtaaag
    55081 aaaatggtag aattgaaaaa agaagcagta aaagacgtaa catcattgac aaaagcagcg
    55141 ccagtagcat tggcaaaaac aaaggaagtc ttgaaccaag ctgttgctga tttgtatgta
    55201 gctcacgttg ctttgcacca agtgcactgg tatatgcatg gtcgtggttt ccttgtatgg
    55261 catccaaaaa tggatgagta catggaagct cttgacggtc aattggatga aatcagtgaa
    55321 cgcttgatta cactcggtgg aagcccattc tctacattga cagagttcct tcaaaatagt
    55381 gaaatcgaag aagaagctgg tgaataccgt aatgttgaag aaagcttgga acgtgttctt
    55441 gttatctacc gttacttgtc agaacttttc caaaaaggtt tggatgtcac tgatgaagaa
    55501 ggtgacgatg tgacaaacgg tatctttgca ggcgctaaaa ctgaaacaga taaaacaatt
    55561 tggatgcttg cagccgaact tggacaagca cctggtttgt aagaaataag gaaacataga
    55621 taaatctgta ggaaatttct tacagatttt ttctattttg taagctattc caaaccagtc
    55681 tttttttgag ttttttgata aaatagtact atcagtgaaa aggatggaag catgactaag
    55741 aaaatcgtag ctatttgggc ccaggataaa gagggtgtga ttggtaagga aaatcgtctg
    55801 ccttggcatt tgccagcaga attgcagcac tttaaagaaa caactctgaa tcatgctatc
    55861 ttgatggggc gtgtgacctt tgatgggatg gggcgtcgct tgcttccaaa acgggaaacc
    55921 ctgattttga cgcgtaatcc ggaagaaaag atagatgggg ttgctacttt tcaggacgtc
    55981 cagtctgttc ttgattggta tcaggctcaa gaaaagaatc tctacattat cggtgggaag
    56041 caaatttttc aggcttttga agcttacctt gatgaagtga ttgtcactca cattcatgct
    56101 cgggtggaag gagataccta tttccctgga gagcttgact tgtctctttt tgagacagtt
    56161 tcaagcaaat tttacgccaa agatgagaag aatccttatg attttaccat ccaataccgc
    56221 aagagaaagg aagtctaatg gaacggagtt tatttggtct attcacggct tttctgtgtt
    56281 tcatctgctt tctggcagga gcacaagctt ttcgtaaaaa gcgttacggg ctttctatcc
    56341 tcctttggtt aaatgccttt accaatctgg tcaatagtat tcatgctttt tacatgacct
    56401 tattttagat agaatgacag tatagaatgg aaggaaatca tgtctacaaa tagaaaaaat
    56461 gatatgatgg tttattgctc attttgtggc aaaaaccaag aagaagtaca aaaaataatt
    56521 gctggcaaca atgcttttat ttgtaatgaa tgcgtggagt tagctcagga aatcattcga
    56581 gaagaattgg ttgaggaagt cttggcagac ttgtctgagg tgccaaaacc aattgaactc
    56641 ctccatatct tgaaccacta tgtaattggt caagatcgtg ccaagcgtgc cttggcagtg
    56701 gcggtttata atcactacaa acgcatcaat ttccacgata cacgcgaaga gtcagaagat
    56761 gtggatttgc agaagtcaaa cattttgatg attggcccaa ctggttcagg gaaaactttc
    56821 cttgcccaga ccttggctaa gagcttgaat gtgccttttg ctattgcgga tgcgacagct
    56881 ctgacggagg ctggttatgt gggtgaggat gtggaaaata tcctcctcaa actcttgcag
    56941 gttgctgact ttaacatcga acgtgcagag cgtggcatta tctatgtgga tgaaattgac
    57001 aagattgcca agaagagtga gaatgtgtct atcacacgtg atgtttctgg tgaaggggtg
    57061 caacaagccc ttctcaagat tatcgaggga actgttgcta gcgtgccgcc tcaaggtgga
    57121 cgcaaacatc cacaacaaga gatgattcaa gtggatacaa aaaatatcct cttcatcgtg
    57181 ggtggtgctt ttgatggtat tgaagaaatt gtcaaacaac gtctgggtga aaaagtcatc
    57241 ggatttggtc aaaacaataa ggcgattgac gaaaacagct catacatgca agaaatcatc
    57301 gctgaagaca ttcaaaaatt tggtattatc cctgagttga ttggacgctt gcctgttttt
    57361 gcggctcttg agcaattgac cgttgatgac ttggttcgca tcttgaaaga gccaagaaat
    57421 gccttggtga aacaatacca aaccttgctt tcttatgatg atgttgagtt ggaatttgac
    57481 gacgaagccc ttcaagagat tgctaataaa gcaatcgaac ggaagacagg ggcgcgtgga
    57541 cttcgctcca ttatcgaaga aaccatgcta gatgttatgt ttgaggtgcc gagtcaggaa
    57601 aatgtgaaat tggttcgcat cactaaagaa actgttgatg gaacggataa accgatccta
    57661 gaaacagcct agaggtgact atggaactta atacacacaa tgctgaaatc ttgctcagtg
    57721 cagctaataa atcccactat ccgcaggatg aactgccaga gattgcccta gcagggcgtt
    57781 caaatgttgg taaatccagc tttatcaaca ctatgttgaa ccgtaagaat ctcgcccgta
    57841 catcaggaaa acctggtaaa acccagctcc tgaacttttt taacattgat gacaagatgc
    57901 gctttgtgga tgtgcctggt tatggctatg ctcgtgtttc taaaaaggaa cgtgaaaagt
    57961 gggggtgcat gattgaggag tacttaacga ctcgggaaaa tctccgtgcg gttgtcagtc
    58021 tagttgacct tcgtcatgac ccgtcagcag atgatgtgca gatgtacgaa tttctcaagt
    58081 attatgagat tccagtcatc attgtggcga ccaaggcgga caagattcct cgtggtaaat
    58141 ggaacaagca tgaatcagca atcaaaaaga aattaaactt tgacccaagt gacgatttca
    58201 tcctcttttc atctgtcagc aaggcaggga tggatgaggc ttgggatgca atcttagaaa
    58261 aattgtgagg aaaagaaaat ggcaaaaaca attcatacag ataaggcccc aaaggctatc
    58321 gggccctatg ttcaaggaaa aatcgttggc aaccttttgt ttgctagcgg tcaagttccc
    58381 ctatcccctg aaactgggga aattgtagga gagaatatcc aagaacagac agagcaagtc
    58441 ttgaaaaaca tcggtgctat tttggcagaa gcaggaacag actttgacca tgttgtcaaa
    58501 acaacttgtt tcttgagcga tatgaacgac tttgttcctt ttaatgaggt ttaccaaacg
    58561 gccttcaaag aggaattccc agctcgttca gctgtggagg tagctcgtct tcctcgtgat
    58621 gtaaaagtcg aaattgaagt catcgcagag attggataag ctagttgaag tttggtgttg
    58681 ccaaacttct tttgatataa ggagaaaaag atgacaaaga aacaacttca cttggtgatt
    58741 gtgacaggga tgagtggcgc agggaaaact gtagccattc agtccttcga ggatctaggt
    58801 tatttcacca ttgataatat gccgccagct ctcttgccta agtttttgca gctggttgaa
    58861 attaaggaag acaatcctaa gttggccttg gtagtggata tgcgtagccg ttctttcttt
    58921 tcagagattc aagctgtttt ggatgagttg gaaaatcaag atggtttgga tttcaaaatc
    58981 ctctttttgg atgcggctga taaggaattg gtcgctcgtt acaaggaaac cagacggagt
    59041 cacccactag cagcagacgg tcgtatttta gatggaatca agttggaacg tgaactcttg
    59101 gcacctttga aaaatatgag ccaaaatgtg gtggatacga ctgaactcac tccacgtgag
    59161 ctgcgcaaaa cccttgcaga gcagttttca gaccaagaac aagcccagtc tttccgtatc
    59221 gaagtcatgt ctttcggatt taagtatgga atcccgattg atgcggactt ggtctttgat
    59281 gtccgtttct tgccaaatcc ctattattta ccagaactga gaaaccaaac gggtgtggat
    59341 gaacctgttt atgattatgt catgaaccat cctgagtcag aagactttta tcaacattta
    59401 ttggccttga ttgagccgat tctgccaagt taccaaaagg aaggtaagtc cgttttgacc
    59461 attgccatgg gatgtacggg tggacaacac cgtagtgtgg catttgctaa atgcttggcg
    59521 caggacttat ccaagaattg gtctgttaat gaagggcatc gcgacaaaga ccgcagaaag
    59581 gaaacggtaa accgttcatg agaaaaccaa agataacggt gattggtgga gggactggaa
    59641 ttcccgtcat tctaaaaagt ctgcgggaaa aagatgtgga aatcgcagct atcgtgacgg
    59701 tggcagatga tggtggttct tcaggtgaac tccgaaaaaa tatgcaacag ttgacaccgc
    59761 caggtgatct tcgtaatgtc cttgtggcca tgtcggatat gcctaagttt tatgagaagg
    59821 tctttcagta tcggttctct gaggatgccg gagcctttgc tggccatcca ttgggaaatc
    59881 tcatcattgc tggcttgtca gaaatgcagg gttcaaccta taatgccatg cagttattga
    59941 gcaaattttt ccatacaaca gggaaaattt atccttccag tgaccatcct ttgacccttc
    60001 atgcagtctt tcaggatggg acagaagtgg ctggagagag tcatattgta gaccatcgag
    60061 gcataattga caatgtctat gtgaccaatg ccctaaacga tgatacgcct ctggccagcc
    60121 gtcgagtagt gcagaccatc cttgaaagtg acatgattgt cctagggcca ggttccctct
    60181 ttacctctat tttgcccaat atcgtgatta aggaaattgg gcgggctctt ttggaaacta
    60241 aggcagaaat tgcctatgtc tgcaatatca tgacccaacg tggggagacg gaacacttta
    60301 cagatagcga ccacgtggaa gtcttgcatc gtcaccttgg tcgccctttt atcgacactg
    60361 tcttggtgaa tattgaaaaa gtgcctcagg aatacatgaa ttccaaccgt tttgatgaat
    60421 acttagtgca agtggaacac gattttgtag gtctttgtaa gcaagtttcg cgcgtgattt
    60481 catctaactt ccttcgtctg gaaaatggcg gtgccttcca cgatggagat ttgattgtgg
    60541 acgagttgat gcgcattata caggtgaaaa aatgagtttc acagtagcag taaaagaaga
    60601 aatcctgggc caacaccacc tgagccggca tgaattatct gccattatca aaatgtctgg
    60661 tagcatcggt ctctcgactt cgggcttgac tttgtcagtt gtgacagaaa atgccaaact
    60721 ggcccgtcac ctctatgagt cctttctcca tttctatgaa atcaaatctg aaattcgtca
    60781 ctaccaacgg agcaatcttc gcaagaatcg cgtctatacc gtttttacag atgaaaaggt
    60841 gcaggatttg ttaagtgatt tgcacttggc agactctttc tttggcctgg aaacaggtat
    60901 tgatgaggcg attttatcgg acgaggaagc aggtcgtgcc tatctctgtg gcgctttctt
    60961 ggcaaatggg agcattcgtg accctgagtc aggcaagtac cagttggaaa tcagttctgt
    61021 ttatctggac cacgcgcaag gaattgcctc ccttctccaa cagtttttac tggatgccaa
    61081 ggtgcttgag cgcaagaagg gggctgtgac ctatctccag cgagcagaag acattatgga
    61141 cttcttgata gtaatcggag ccatgcaggc acgtgatgat tttgagcggg ttaagatttt
    61201 gcgagaaacc cgtaacgacc tcaatcgggc taataatgcc gagacagcta atatcgctcg
    61261 gacagtttct gccagcatga agaccattaa caatatcagt aaaatcaaag atatcatggg
    61321 cttagaaaat ttgccagtag atttgcagga agtggcgcaa ctgaggattc agcacccaga
    61381 ctactctatc cagcagttag cagatagtct cagcacccct ctgaccaaaa gtggtgtcaa
    61441 tcacagactc agaaaaatca ataagatagc ggatgaatta taaaaccacg aatcctatgt
    61501 gactcgtggt tcttttttat aaactagtag agtgttttgg ttgtactttt tgttcagggt
    61561 caatgtagtt gatagcgttg ttgacagcag ttggagcttc tccgaggcct gtcgcaatca
    61621 gatcaatttt tccgtcatag tagcagcagt caccgatagc atagatacct gcttggctgg
    61681 attcctgttt gctgttgacg ataatcttgt gacggttgag gtcgagcccc cagtttttaa
    61741 ggttaccgac agaagatttg aaaccatagt tgacaaagag gtggtctagg tcaatggttt
    61801 cagtttcatc agatttgact tttgtgattt caagtttatc aagtgttttt ccatttccaa
    61861 ggagttggct aggggcgaat ggtgtcttga tggttacaga tgattcttgc aaggcttgaa
    61921 cactgtgttc caaggcacgg aaattatctc tgcggtgaac aagggtagtt ggtgcgattt
    61981 tttcaaaagc caaagcccaa tccacagccg agtctccccc accaagaatc gtcactttct
    62041 taccagcgta ttgctgaatg ttagaaacgt ggtagtggat attttcatag ccctcaaccc
    62101 cttcaagttc cagcggacgt ggtttgaagg caccgccacc catagcgatg ataactgttt
    62161 tagtcaggtg acttccttta gaagttgtga tggcaaattc ttcttgtttg tcaatctcaa
    62221 gaaccgtttc attgagatga ataggggtat caaatccatt tagctgttca atcaagcggt
    62281 tagtcaactc ttctccagtc aggtttggga agcctggtac gtctaggatt tccttttcag
    62341 ggtagagaat agcaggttgt ccacctagct ggggaagaga gtcgatgatt tgaaccttgg
    62401 cttggcgtag gtgggcataa aaggctgcaa aaagcccgac aggaccacca cccacaatgg
    62461 taatatcata gagttgagac atagtttctc ctttgttttt tctagtcagt ttattttatc
    62521 atatttttgt tcaatgtaaa atggactagg atagggaaaa aaatgccaga aaatggtata
    62581 ataggacgaa agtgttttga tagggaggat atcagggatg aattttcaac aattatccaa
    62641 tctgcaatat tggaccagtt tgtttgcaag tccatggacg atagctatca atctgattga
    62701 tattttgatt gttgcttata ttttatacca ttttacaaaa gctattgcag gaaccaagat
    62761 tatgattttg gtacgtggag ttttggtgtt tattttagct caaatccttg caaatatgat
    62821 tggtttgact acgatttctt ggttaatcaa tcaaattatt acttatgggg ttattgcggc
    62881 ggttgttatc ttctctccag agattcggac tggtttggaa cgtttgggaa gagcgacaga
    62941 ttttttttcc aatgccccta ttagtgctga ggaacagatg attcgtgcct ttgttaagtc
    63001 tgttgaatac atgagtcctc gtaaaatcgg ggccttggtt gctattcagc gtgtacgtac
    63061 cttgcaggag tatatttcga caggaattcc cttggatgct aagatttctg cagaacttct
    63121 cattaacatt tttattccca acactcccct acatgatggt gcggtgatta tcaaagaaga
    63181 acgtatcgct gtgacgtctg cctatctgcc cttgacaaaa aacacaggga tttccaagga
    63241 atttgggacc agacaccggg cggctatcgg tttatcagaa gtctcagatg ccttgacttt
    63301 tgtcgtatca gaggaaacgg gaggaatttc gataacctat aatggaaggt ttaagcacaa
    63361 cctaacactt gatgaatttg aaacagaatt acgtgaaatc ttacttccaa aagaggaagt
    63421 gggtcttagt tttaaagaac gattgctagg aggatggaaa catgaaaaaa aatagtttat
    63481 atatcatatc ctcactcttt tttgcttgtg tcttatttgt ctatgctacg gcgacgaatt
    63541 ttcaaaacag taccagtgct aggcaggtaa aaacggaaac ctatactaat acagtaacaa
    63601 atgtccctat tgacatacgc tataatagtg ataagtattt tattagcggt tttgcttcag
    63661 aagtatcagt ggtcttgact ggggcaaatc gcctatcgct agctagtgaa atgcaagaaa
    63721 gtacacgtaa attcaaggtt actgctgacc taacagatgc cggtgttgga acgattgaag
    63781 ttcctttgag cattgaagat ttacccaatg ggctgaccgc tgtagcgact ccgcaaaaaa
    63841 ttacagtcaa gattggtaag aaggctcaga aggataaggt aaagattgta ccagagattg
    63901 accctagtca aattgatagt cgggtacaaa ttgaaaatgt catggtgtca gataaagaag
    63961 tgtctattac gagtgaccaa gagacattgg atagaattga taagattatc gctgtcttgc
    64021 caactagcga acgcataaca ggtaattaca gtggttcagt acctttgcag gcaatcgacc
    64081 gcaatggtgt tgtcttaccg gcagttatca ctccgtttga tacaataatg aaggtgacta
    64141 caaaaccagt agcaccaagt tcaagcacat caaattcaag tacaagcagt tcatcggaga
    64201 catcttcgtc aacgaaagca actagttcaa aaacgaatta aaaatagaaa aggattttat
    64261 aacaatgggt aaatattttg ggactgatgg agtccgtgga gaagctaacc tagaactaac
    64321 accagaatta gcctttaaac taggacgttt tggaggctat gttcttagtc aacatgaaac
    64381 ggaagcgccg aaagtctttg taggacgtga cacacgtatt tcaggggaaa tgctggaatc
    64441 ggccttggtg gcaggtctcc tttcagtagg gattcacgta tacaaacttg gtgtccttgc
    64501 aacaccagca gtagcttact tggttgaaac tgaaggagca agtgccggtg tcatgatttc
    64561 tgctagccac aacccagccc ttgataacgg aatcaagttc tttggcggtg atggcttcaa
    64621 actagatgat gaaaaagaag cagaaattga agccttgcta gatgctgagg aagacactct
    64681 tcctcgtcca agtgcagaag gcttaggaat tttggtagat tatccagaag gcttgcgtaa
    64741 gtatgaagga taccttgttt caactggaac tcctcttgat ggaatgaagg ttgccttgga
    64801 tacagctaat ggagcagctt ctaccagtgc ccgtcaaatc tttgcagacc ttggtgccca
    64861 attgacggtt atcggggaaa caccagacgg tcttaacatc aaccttaatg ttggttcaac
    64921 acatccagaa gcccttcaag aagtggtcaa agaaagtggg tcagctattg gtttggcctt
    64981 tgatggagac agtgaccgct tgattgctgt tgatgagaat ggtgacatcg tcgatggtga
    65041 caagattatg tacatcatcg gaaaatacct ttctgaaaaa ggacaattgg ctcaaaatac
    65101 aattgtgaca actgttatgt ctaaccttgg tttccacaag gccttgaatc gcgaaggtat
    65161 taacaaggca gttactgcag ttggtgaccg ctacgttgtt gaagaaatga gaaaatcagg
    65221 ctacaacctt ggtggtgaac agtctggtca cgttatcttg atggattaca ataccacagg
    65281 tgatggtcaa ttatcagcag ttcaattgac taaaatcatg aaggaaactg gtaagagctt
    65341 atcagagttg gcggcagaag taacgattta tccacaaaaa ttagttaata tccgagtgga
    65401 aaacgtcatg aaggaaaagg ccatggaagt gccagctatc aaggccatca tcgagaagat
    65461 ggaagaagaa atggcgggga acggccgtat ccttgttcgt ccaagtggaa cagagcccct
    65521 cttgcgtgtt atggcagaag cgcctacaac agaagaagtg gactactatg ttgataccat
    65581 cacagatgta gttcgtgctg aaattgggat tgactaaatc ctagaaaact agatgaaaat
    65641 aaaaaattat ggtacaatag cttataatat tgtagccgat ggagaaagac atgaatctta
    65701 aacgtgaaca agaatttgtt agtcagtatc attttgatgc gcgtaatttt gaatgggaaa
    65761 atgaaaatgg agctcctgaa accaaggtgg atgtgaattt ccaattgctc caacatgacc
    65821 aagaaaatca agtgacttcc ttgattgtca tcttgagctt tatgattgta tttgataagt
    65881 ttgtcattag tggaacaatc tcacaagtaa accatatcga tggccgtatt gtcaatgaac
    65941 caagtgaatt gaaccaagaa gaggtggaaa ccttggctcg tccatgtttg aacatgctca
    66001 atcgtttgac ttacgaagtg actgaaattg cattagactt accaggaatt aatttggagt
    66061 tctaatatga aattagctgt tttcacagat tcctctgctt atctcagtga agagaccttg
    66121 caaagagaag acttgtttgt cttggatatt cctgtcaata ttgatggtga ggaatatgtc
    66181 gaaggcatca atctgtctgc tgaggaattt taccaaaaaa tggctcaggc ttctgaattg
    66241 cctaagacca gtcaaccaag tattgccaag ttagatgaga tcttaacttc gctcaaagaa
    66301 caaggctata cacatgcctt ggggcttttc ctatcttctg gaatttcagg tttttaccaa
    66361 aatatccagt atatggtcga tgactatgag ggcttaacca ttgctttccc agacactttg
    66421 attacaagtg ctcccctagg catcatggtt gaaagcgtct ttaattggcg tgatcagggc
    66481 gatgattttg ccagcattca ggataagcta gccattcaaa tcagccgtac gtcagccttt
    66541 atcatggtag atgacttgga tcatttggta aaaggtggac gcctttcaaa tggggctgcc
    66601 attttgggca atttgcttag cattaagcca atcctttatt ttaacgatca aggtgtgatt
    66661 gaagtttacg aaaaagttcg tactgaaaag aaggccacca aacgcttaat tgaaattatc
    66721 aaggaaacaa cggcttcagg tcaataccgg gtcattgtca ttcatgggaa tgatcctgaa
    66781 aaggctgaag aattgcgtca gcatttgctt gattttggct tgggttcgga tgtttcactt
    66841 gctacatttg gtagtgtcat tggaacgcac ctaggagcag gaagtattgc tctgggttat
    66901 attccagtga tttagtaggc acttctaggc tagttaatct tagcaattga acacggacta
    66961 cctgcctctt gaaaaaagat gtctgtcttg cctttcgtgg aaagtcagtg ccattcccta
    67021 tttttcatgg gtggctaaga tcctttgtat tttgataatt gaacacgcct ggaaccctgt
    67081 gtgaaaaaga tagttcttcc aaggagtaga cacttcttga tcagaattcc tattttcact
    67141 ttgtgttctt acaggctttg tatcttagac aggagtagag atgagtattc gagtaattat
    67201 tgccggtttt aagggaaaga tgggtcaagc tgcttgccag atggtcttgc ctgattcaga
    67261 tttggacttg gtagcagttt tggatccttt tgagtctgag tcagaatggc agggtattcc
    67321 tgttttcaag gataaggctg atttagctgg ttttgaagcg gatgtctggg tagactttac
    67381 tactccagct gttgcctacg aaaatacacg ttttgctctt gaaaatggct ttgctccagt
    67441 agttggaacg actggtttca cgagtgaaga aattgcagag ttaaaagaat tttctcgtgc
    67501 ccaagacttg ggtggcctga ttgcccctaa ctttgccttg ggtgctgtct tactcatgca
    67561 atttgcgacg caggctgcca aatatttccc aaatgtggag attattgagc tccatcatga
    67621 caagaaaaag gatgctccga gtggaacagc cattaaaaca gctgagttga tggcagaggt
    67681 tcgagagtcc attcagcaag gcgcagcaga tgaggaagag ctgattgctg gtgctcgtgg
    67741 tgctgacttt gatggtatgc gcatccactc agttcgtttg ccaggtttgg tagcccatca
    67801 ggaagtcatc tttggcaatc aaggagaagg attgaccctc cgtcatgact cctatgatcg
    67861 catctccttc atgacaggag tcaatttggg aattaaagaa gttgtcaagc gtcatgagct
    67921 tgtctatgga ttagaacact tattatgaga ttaacgcaaa tgccttctga atttcagaag
    67981 gctttaccag tattagaaaa aattaaagaa gcaggctttg aggcttattt tgttgggggc
    68041 tctgttcgag atgcccttct ccatagccct atccacgatg tggatattgc gacgtcttct
    68101 tatccagaag agaccaagca gatttttccg cgaacagccg atatcggaat cgagcatgga
    68161 accgtcttgg tcttagatgg ggatgaggag tatgaggtaa caacctttcg gacagaggat
    68221 gtctatgtgg actatcgcag acccagtgcg gtttcctttg tgcgctcgct agaagaagac
    68281 ctcaaacgcc gtgatttcac agtcaacgcc tttgccttgg atgagacagg agaaatcgtt
    68341 gacttgttcc atggtttaga agatttggaa aagcaagtct tgcgagcagt tggagtggct
    68401 agtgagcgtt tcaacgaaga tgctttacgg attatgcgtg gtttccgttt tcaggctagt
    68461 cttggttttg cacttgagcc agaaacattt aaagctatga agaccttgac gccgcttttg
    68521 gagaacattt ctgtagagcg taccttcgtc gagtttgata aactcttgct ggctccattt
    68581 tggagaaggg gcttggcttc catgattgag agtcaagctt atgactatct ccctgatatg
    68641 gcatctagcc agaacaagct caacagactg tttgatttgg agactgattt tacttttgaa
    68701 tcctctgaac aagcctgggc ggctttacta tgggctttgg agattgaaaa tgcgcagtca
    68761 tttttgaaat cttggaagac ctcacgccag tttgccaagc aagttcagga tttgctgatt
    68821 attttggctc tgcgtgaaaa tggagaattg agcaagcgag attgttatcg ctttgacata
    68881 gatttgcttt tacaggctga aaatcttcgt caagctcgag gaaaagaagt caacccacaa
    68941 gccatcacag aaaaatacca aagcttgact atccatgaca agaaagagat tcagattaat
    69001 ggcgatattt tgatcaagga atatggctat cagccaggcc cagacttggg agagatttta
    69061 acagagattg agtttgccat tgtcgatgga gaattggaga ataatcgtga agccatccat
    69121 gcttacctga gggagaaaaa atgagtgatt ttatcgttga aaaactaagt aaatccgttg
    69181 gtgacaagac cgtttttagg gatatttcct ttattatcca tgacttagac agaattggtt
    69241 taatcggtgt caatgggact ggcaagacca cccttttgga cgtcctttct ggtgtttctg
    69301 gctttgatgg ggatgtcagt cctttttcag ctaaaaatga ttaccagatt ggttacttga
    69361 ctcaggatcc tgattttgat gatagaaaga cagttttgga tacggttcta tctagtgaac
    69421 tcaaggaaat ccagctcatt cgtgagtatg aattgattat gctcgactat agtgaggaca
    69481 agcaggcgcg tttggaacgt gtcatggcag agatggactc tctccaagct tgggaaatcg
    69541 agagtcaggt caagaccgtt cttagcaaat tgggcattca agacttatct actcctgttg
    69601 gggaattgtc aggtggtctg agaagacggg tacagttggc acaagtctta cttggcaacc
    69661 acgacctctt gcttttggat gagccgacca accatctgga tattgcgatt attgagtggc
    69721 tgaccctctt tttgaaaaat tctaagaaga ccgtcctttt tatcactcac gatcgttatt
    69781 tcttagacgc tttgtcaaca cggattttcg agttggagcg agcaggcttg acagaatatc
    69841 agggcaatta ccaggactat gttcgcctta aggcagaaca ggacgagcgc gatgcaactc
    69901 tacttcataa aaaagaacaa ctctacaaac aagaactggc ctggatgcgc agacaaccgc
    69961 aggcacgtgc gaccaagcaa caagctcgta tcaatcgttt ccatgatctg aaaaaggaag
    70021 tttcaggcag tagtgctgag acagacttga ctatgaactt tgaaaccagt cggattggga
    70081 agaaagtcat cgagtttcag gatgtttcct ttgcctatga aaataagccc attttgcaag
    70141 attttaatct cttggtacag gtcaaagatc gtatcggaat cgttggggac aatggtgttg
    70201 gaaaatcaac cctacttaac ctgattgcag gaagtcttga gccgatagca ggacaagttg
    70261 tgattgggga aactgttcgc atcgcctatt tctctcaaca aattgagggt ttggatgaaa
    70321 gcaagcgtgt gatcaattac ctgcaggaag tggcagagga ggtcaagacc agtggtggtt
    70381 ctacgacttc catcgctgag ttgctggagc aattcctctt cccacgttcg acgcatggga
    70441 ccttgattga gaaattatct ggtggagaga aaaaacgtct ttatctcctc aaactgctct
    70501 tggagaaacc aaatgttctt cttttagacg agccaaccaa tgacttggat attgcaactt
    70561 tgacagtctt agagaatttc ttgcaaggtt ttgcaggtcc cgttttaaca gtcagtcacg
    70621 accgctattt cttggataag gtagcgacca agattctcgc ttttgaggat ggcaagattc
    70681 gtcctttctt tggtcattac accgactatc ttgatgaaaa agcttttgaa acagatatgg
    70741 ccaatcaagt gcaaaaggcc gaaaaggaaa aagtggtcaa ggttcgagaa gacaagaaac
    70801 gcatgaccta ccaagaaaag caggagtggg caagtattga aggtgatatt gaaaccttgg
    70861 aaaaacgtat cgctgctatt gaagaggaaa tgcaggctaa cggctctgac tttggtaagc
    70921 tggctactct ccaaaaagaa ttggatgaga aaaatgaagc actccttgaa aaatacgaac
    70981 gctatgagta tctcagtgaa tttgatagtt aagactattc gaaaatcaaa gtgcaaacta
    71041 tgtcggcttc acattgccga ctcaagtaca gcctgtgacc ggcttcctaa tttgcacttt
    71101 gatttttatt gagtataaaa gaatagaaaa atcccgtctc atgaaaggat ttctctattc
    71161 ttttttggtt tcttgatgaa agatattttg ccaggtttcg tggcgacgcc agatactggt
    71221 atggatgata ccatctaact catagcagat gagtttggtt ttttgactga tggaagtaat
    71281 ttgaatgtcc ttgatagcag aatttaactc ttttttggtc ttataagcct ctttatccat
    71341 ctgctctcca tcttggcgaa tatagacaaa atcatcagtc aagagttctt ctagttggtt
    71401 tccttggtca atcaattgtt cacgcataag caatttttta tagacattgt ctaaaatttc
    71461 atcctcaggg ataggtgctg gttcgatatg gacatcggta tcaaagacgc caaaacgctc
    71521 ctccagcata gactcgacct gatccgcgat ttcatggctt tcaaaaacag acaagtcagg
    71581 attcatctct agtgtaatat ccaggtagat gttgctaccg taggtgcgac ctctttgcga
    71641 tttgaccttg ctgattttgg gaatttccat gatagccttt tggtagtcct cgagcaggcg
    71701 gtcgtcaaag ccatctgaaa gactaaagga agactcgatg aagatatcat aggcagtctt
    71761 caagataaag aaagtgatga tgatagcaac cagtttatcc acaatcggat aattgaaact
    71821 actagctagg atggcaatgg cggttccaag tgaggtaaca gcgtcagaaa gattgtcctt
    71881 agcagctgcc ttcagcgcct tggagttgga tttcttactg aggcgagtat tgtagagata
    71941 gaccacaaac ataatcgctg cagaaatgat tcctagagtt gcaccaagag gatcaatgac
    72001 cgtttcttcc cgactgagaa tcttttgaat ggtatctctt agaatatcga aaccgacata
    72061 gaacatgatg atagaagtga tcaagcttgc caaatcttca atcttccaat gaccaaaacg
    72121 gtggtcacgg tctgcaggct ggcgcgccat ccgaatcccg attaagaggg ccacatttcc
    72181 aatgatgtcc gatacgttat taaaaccatc ggccaccaaa ctggatgaat gaaggagatg
    72241 accagctgct aatttagctg cagacaagat caaataggtc gaaatactga taatggctcc
    72301 acgctcagct aacttgagat ttgagataga ttgcttcatt caacttcctt tctagtcaca
    72361 tagcattcta ccattatact ggttttcaag gaaaaattca aataaggcag tttttactct
    72421 ttgaaaactc cccaaaattt ggtataatgg tactactcaa gggagtagct ggcagaaacc
    72481 tgtgatagtg tcgtcattcc gaattttatg ctgaaaagta tgctttccgg ccctatctta
    72541 aacagcgaga cttgttatga ttaacaggtc tctttttgtt tgtcgtgaaa agatatgaca
    72601 gaaagagagt ctgttttgca cacaatgtca aggaggagac acatgtcaaa agaacaaaaa
    72661 cgccaagcgt tttatactca gagccctgaa gaggtcttgc aggctgtgga tgcgaccgag
    72721 caaggtttgt catcaagtga ggcggaaaag cgccttgccg aatttgggca caatgaactc
    72781 gaagaaggcg agaaacgatc aatcttggtc aaattcatcg agcaatttaa ggatttgatg
    72841 attatcatcc tagttgcggc agcaatcttg tcagtcgtga cttctggtgg ggaagatatc
    72901 gcagatgcca ttatcatcct agctgtggtt atcatcaacg ctgcctttgg tgtttaccaa
    72961 gaaggaaaag ctgaagaagc tatcgaagcc ctcaaatcca tgtctagtcc agttgcccgc
    73021 gttcttcgtg atggacacat ggcagagatt gactctaaag aattggtacc aggcgatatc
    73081 gttgcccttg aagcaggtga cgtggtacca gcggacctac gtttgctaga agccaactct
    73141 cttaaaattg aagaagcagc cttgacgggt gaatctgtac cagtcgaaaa agacttgtca
    73201 gtcgagcttg cgacagatgc tggtattggt gaccgtgtca acatggcctt ccaaaactca
    73261 aacgtaacct atggtcgtgg gatgggtgtt gttgtcaata caggtatgta cactgaagtt
    73321 ggtcatattg ctggtatgct tcaagatgcg gatgagactg atacaccact caaacaaaac
    73381 ttgaacaacc tttctaaggt cttgacctat gctatcttgg tcattgccct tattactttt
    73441 gtagtgggtg tcttcattca agggaaaaat ccacttggtg agttgttgac ttctgttgcc
    73501 cttgccgttg cagccattcc agaaggactt cctgctatcg ttaccatcgt tctttctctt
    73561 ggtactcaag ttttggccaa acgacattcc atcgttcgta agttgccagc agttgaaaca
    73621 cttggttcaa ctgaaatcat cgcttctgat aagactggta cgctgactat gaacaagatg
    73681 acagtcgaaa aagtcttcta cgatgcggtt ctacatgact cagctgatga tattgaacta
    73741 ggtcttgaaa tgccactact tcgttcagtt gtcttggcca atgatacgaa aatcgatgtg
    73801 gaaggtaact tgattggtga cccaaccgaa acagccttta tccaatatgc cttggacaag
    73861 ggctatgatg tcaaaggttt cttagagaaa tatcctcgtg tagctgaatt gccatttgac
    73921 tctgaccgta agctcatgtc aacagttcac ccattgccag atggtcgttt ccttgtagca
    73981 gtcaagggtg cgccagacca actcttaaaa cgttgtcttc ttcgtgataa ggctggggat
    74041 attgctccga ttgatgagaa ggttacaaat ctcattcata caaacaattc tgaaatggct
    74101 catcaagcct tgcgtgtcct tgcaggtgct tataagatta tcgatagtat tccagaaaat
    74161 ctcacttctg aagagcttga aaatgattta atttttactg gtttgattgg gatgattgac
    74221 cctgaacgtc ctgaagccgc tgaggctgtt cgtgtggcta aggaagcggg aatccgtccg
    74281 attatgatta caggtgacca ccaagacaca gcggaagcca ttgccaaacg tttgggaatc
    74341 attgacgcaa acgatacaga aggtcacgtt ttaactggtg ctgaactcaa tgaactgtca
    74401 gatgaagaat ttgaaaaagt cgttggtcaa tactctgttt atgcccgtgt gtctccagaa
    74461 cacaaggttc gtatcgtcaa ggcttggcaa aaacaaggta aagtcgttgc catgacaggt
    74521 gacggtgtca atgacgcgcc agctctgaaa acagccgata tcggtatcgg tatgggaatc
    74581 actggtacag aggtttctaa gggggcttct gatatgattc ttgcagatga taactttgcg
    74641 actattatcg tcgcagtgga agaaggacgt aaggtcttct caaacattca aaagactatt
    74701 cagtacctac tttctgctaa tactgctgaa gtattaacca tcttcctatc aaccttgttt
    74761 ggttgggatg tcttacagcc ggttcatctt ttgtggatca acttggtaac ggataccttc
    74821 ccagctatcg ctcttggtgt tgaacctgcg gaacctggtg tcatgaatca taaaccacgt
    74881 ggacgcaagg caagcttctt ctcaggtggt gttttgagtt ctatcattta tcaaggtgta
    74941 ctccaagcag ctcttgttat gagtgtttat ggccttgcga ttgcttaccc agttcatgtg
    75001 ggtgacaatc atgctattca tgcagatgcc ctaacgatgg cctttgcaac ccttggtttg
    75061 attcagctct tccatgccta caatgtcaag tctgtctacc aatccatctt gacagttggc
    75121 ccattcaagt ctaagacctt taactggtcc atcttggtat cctttatcct tctcatggca
    75181 acaatcgttg tagaaccgct tgaaggaatc ttccacgtaa ccaaactaga cttgtcacaa
    75241 tggggaattg ttatggctgg aagcttctca atgattatca tcgtcgaaat cgttaagttt
    75301 attcaacgca aactcggttt tgacaagaat gcgatttaag ggaggtttcg gatgtcagca
    75361 tatcaattac cgaccgtatg gcaggatgaa gctagtaatc aaggagcttt tacggggcta
    75421 aacagaccaa cagcaggtgc ccgtttcgaa caaaacttgc caaaaggaga acaagctttt
    75481 cagctttatt cactgggaac accaaatggt gtgaaggtta ctatcttatt ggaagaatta
    75541 ctagaagctg gttttaagga agcggcttac gacttgtata agattgctat catggatggg
    75601 gatcaattcg gctcagactt tgtgaagctc aatccaaatt ccaagattcc agccttattg
    75661 gaccagtcag gtactgaaaa cgtaagagtc tttgagtctg ctcatattct tctttacctt
    75721 gctgagaaat ttggagcctt tttaccaagt aatcctgtgg aaaaggtaga agttttgaat
    75781 tggctattct ggcaagcagg tgcagcacct tttctaggtg ggggatttgg acatttcttc
    75841 aattatgctc ctgaaaaatt ggaatatcct attaaccgtt ttacgatgga agtgaaacgc
    75901 cagttggatt tattggataa ggaattggct cagaaacctt atattgcagg caatgactat
    75961 acgattgcag atattgctat ctggtcttgg tatggacagt tagttcaagg aaatctttac
    76021 caaggttctg caaaattctt ggatgcctca agttatcaaa atctagtaaa atgggcagaa
    76081 aaaattgcca atcgtccagc tgttaagcgt ggcttggaag taacttatac agaaattaaa
    76141 tagcaaaaaa gtcatcttcg gatggctttt tgctacagtt gagggaaagg gtttgcagtt
    76201 atcggctttt gtgctataat tgctataata tagtaaaaag caagaggagt gtgaggaagt
    76261 ggaaaagaaa attgtgaagg atatcttatt tttatctcaa gtgtctcagc cggcaagtca
    76321 ggaggacctt tatcttgcca gagatttgca ggatacactc ttagcaaatc gtgatacctg
    76381 tgttggtcta gctgccaata tgattggggt gcagaagcgc gtgattatct ttaatcttgg
    76441 cttggttccc gtggtcatgt ttaacccagt gcttctgtcc tttgaaggat cctatgaggc
    76501 agaagaaggc tgtttgtcct tggtaggtgt gagatcaact aagcgttatg aaaccacaag
    76561 gcttgcctat cgtgacagca agtggcagga acagaccatt accttgacag gcttcccagc
    76621 tcagatttgc cagcatgagc tggatcactt ggaaggacga atcatttagg aggaaagcaa
    76681 atgaaacgaa tagtctttga acttattttt atcgcaacga cctggtatat ctttttaccg
    76741 ccccttaacc tgaccagctg ggaatttctc ttcttcctct gtgggcattt gttagttgtg
    76801 gcaatcttat ttggctttgg caaggggata aaccttgtca aaacggttca tgtgcgccac
    76861 ggtaaggcgg aagctgcctt aaatcttgag ggtttcaaaa tcaatcggtt agggaaaatt
    76921 ctgttagctt cgattggagg aattcttctc ttggcagctt tggtttcctt ggtaacttcc
    76981 agcatgtttc aggctaaaaa ttatgccaat gtagtcacgg ttacggaaaa agactttact
    77041 gaatttccta ggagtgacac cagtaaggtt cctatcctag atagaagtac tgctgaaaaa
    77101 attggagacc gctacttggg ttccctaacc gataaggtgt cgcaatacgt agcggcagat
    77161 acctataccc aattgacaat tgatgggaaa ccttatcggg tcacaccact agaatatgca
    77221 gaccctatca aatggtttaa caatcaagcc aagggaatcg gtgagtatat caaggtggac
    77281 atggtaactg gaaatgcgga tttggtggac ttgaagacac caatcaagta ttcagactcg
    77341 gagtatttta accgtgatgt caaacgtcac ctgcgcttga agtacccgac taaaatcttt
    77401 aaaactccat cttttgaggt ggacgatgag ggcaatcctt tctatgtagc aacggtttac
    77461 caaaagcaat ttggacttgc ggttcctcgt cctgcttcag tcattatctt ggatgctaca
    77521 aatggagaaa ccaaggaata cagcttatca gatgttccag aatgggtgga caggatctat
    77581 ccagcagagg aaaccattga gcaaatcaac tacaacggca agtacaagga cggtttcttg
    77641 aatgccatga tttccaagaa aaacgtgacc cagactacca atggctataa ttacttgtct
    77701 atcggtaatg acatctatct ctacacaggt gtgacgtcgg ctaatgcgga tgagagtaat
    77761 cttggtttca tccttgaaaa tatgcgaaca ggagaaatta ctaagtatag cttggcttct
    77821 gcgacagaag aatcagcccg tgaatcagca gaaggtgctg ttcaggagaa atcctacaaa
    77881 gcaaccttcc caatcctcat caacctcaat gacaagcctc tctacatcat gggcttaaag
    77941 gacaatgctg gcttggtcaa agagtacgcc ctggtagacg cagtcgagta ccaaaatgtt
    78001 atcgttgcta ctacagtgga agagatgctc agcaagtatg ccaataaaaa cgaccttgaa
    78061 attgacaatg caacgacaga aagcatcaag ggagtagtag cagacctcaa atcagctgtt
    78121 atcaagggag acactgtcta cttctttaaa gttgatggca agatatacaa ggtaaaggct
    78181 tcagtatccg atgaccttcc ttaccttgaa aatggtaaaa ccttcgaagg tcaagtagga
    78241 aaagacaatt atctcaagac ctttaaagta caataatact cttcgaaaat ctctagctct
    78301 atctgcaacc tcaaaacagt gttttgagca gcctgcggct agtttcctag tttgctcttt
    78361 gattttcatt gagtataaaa aaggtttatt taataaatat atgttataat agagtaaatt
    78421 aagactaatg gaggacaaag aaatgggttt ttatttgatg gttgcttcaa tgctactcgg
    78481 actgctcgct ttgaagatag gtttttctca gttcaaggag aaaaaagata aattcttatc
    78541 tatcttaaca agtttagctg gcacagccct tgttctcgtg gctgtctggt taggatggcc
    78601 caaataaata gaaaaacctc ggtcaaaccg aggtttttca aatgaatttc ttggttgtgg
    78661 ctaaaaaata tgctacacta tcaatatgaa aattttaatc ccaacagcaa aagaaatgaa
    78721 cacagacttg ccaagtatcg aggcaattcc tttaaaacca gaaagtcaga ccgtgcttga
    78781 tgccttggct ctctattctg ccagtcaatt ggagagtttc tacaaggtat cagctgagaa
    78841 agcggcggaa gaatttcaaa atatccaagc tttgaaaagg caaactgctc aacactatcc
    78901 agccttgaaa ctttttgatg ggcttatgta ccgcaacatt aagagagata agctgaccga
    78961 ggcggaacaa gattatcttg aaaatcatgt cttcattacc tcggctttgt acggtgttgt
    79021 tccagccttg tcacccatgg ctcctcaccg tttggatttt ttgatgaaat taaaagtcgc
    79081 tggtaagact ttgaagagcc attggaaggc agtctatgat gaagctctga agaaggaaga
    79141 agtgattttc tctctcttgt catcagagtt tgagactgta ttttctaagg aaatcagagc
    79201 aaagatggtg accttcaaat tcatggagga tagaggcggt cagctgaaga ttcactcaac
    79261 tatctccaag aaagcgcgcg gggcctttct aacagcttta atagaaaatc aagtacaaac
    79321 tgtgggggaa gcacgtcgct tgaactttgc tggatttgtt taccgagaag atttgtcaca
    79381 accacaggga ttggtttttg taaaggaagt atagaaatca gataagagaa aagtcagcac
    79441 gattatgctg acttttttga taacttataa agtattggac aaatgaatct catttgataa
    79501 ggtgttaaga tgattaacca aattcatgat tgacagatga tttcggcaaa taaacttctt
    79561 ggtaatccct ggaatataga aattagacac gatgatatcg taatctgtct gatttaaaat
    79621 ctcaggactt gtttttaatt catcccaaat atcgaaggta aagcggttat tacagtaata
    79681 ggaaagcata tcaacaaagg taagggatat agcatggtca aagttactga taatcaaaac
    79741 cttgataggt ggtcgatttt ctaacaactg agtagagagg ttttcagcat gggttaagat
    79801 ggtgtacatc aagtgttcca actgttcagg atggtcatgc tgtccaattg cctgacggta
    79861 ttgagccagt tcttgacgcg cacttcccat aaagtctggg aagtaaactt caaatttttt
    79921 aatcgtcaat gctttttgtt caaataagat tggggtagaa aagatttctt gtctttcaaa
    79981 gaaagcagtg ttgtgtaaat gccaaatcag ctcatcgtgg ttagtaaaag taatcttata
    80041 ttgttttgat aggcgttcta atatttgact aagtaattgg taagagtatc ttgcttgact
    80101 gttatcttct aaagagttga agaattcttg aggatctagg aaaatatcat tctgaagaaa
    80161 agaaataaaa atttgagcga ttgtatctgg agtcatttca agcccaaaat gtttagagaa
    80221 ataagcaagt gtctcctgga aatttggaat gctctttaaa agaggataat attctttgta
    80281 aaagtggttg gggaggtcaa tgaagtgacc atttttaacc ctgtggatac taatagctat
    80341 catcagtttg tacattctat aaattgaaaa tcgcattgga taattcgtga ttttatagaa
    80401 gaagtcagca aactctgtta aatcttcctc aggaaggtca ggaaaaggcc aatctaggaa
    80461 ataatagcgc tcagaaaagt attgggcaaa aaagtaacga atatcgatct catttccacg
    80521 tatctcggat ggagtaaaag ataattcaat ttgaaactga gatgaaagaa cctttgtaat
    80581 atttctgccc agtcgataga ggttggcact actaaagtaa agattttcta ttgttcgata
    80641 aataggtagc ccctcattga agaacatata ctctagtaat tgaaaagatt gagaattagc
    80701 aagaaaatat tgataaatat cttctacact agtgttaact tctaaatcta tcatgattcc
    80761 gttaacagaa gattggatgt taatagatgg aaaggcaata cgtaattcat ttaaatcact
    80821 ttttaagatt cgttctgtac atcctaattt ttcggctagt tcatgtaatt tcatccaatt
    80881 acgattctga attagggttt ccattaagcg taattgtctt tgaacttttg tggataaaag
    80941 gtttctcata cgggtatttc ctccataatt cattatatct ttttttgtct aagtagtata
    81001 aaaaaattag agcaagaaaa ttgtcgttag taatcggaaa aattagaata atataagtgg
    81061 cttttaagtg cagaaacaga gtatattaat tgttcagaaa atattgatta atgcctcaaa
    81121 tagttgtaca ttgtaaacta taaaatacta tctaggagtt taaaatgaaa cattcacata
    81181 aaaaatcatt tgactggtat agcatgcaac aacgttattc tattcgtaag tattactttg
    81241 gtgcagctag tgtcttgctc ggtaccgctt tggtattagg tgtagcagct agtgtccaaa
    81301 cagtacaagc ggaagaaaac aaacaagaaa ctaccaatag tatttctgtt ggtaggggag
    81361 aagcagctac taaaccagca gaggtttctg cgtctaataa agagaaaacc tatgcagctc
    81421 caactgtagc taatccagta gaaacgactc cagttaaaac tgaagaagtt actaaaccag
    81481 cagaaaaagt tgaagaagca aaagacaaaa aagaggaagt aacgcatcaa gatgccgttg
    81541 acaagtcaaa attattaacg gctctttcgc gtgctaaaaa attagaaagc aagttatata
    81601 cagaagcaag tgctgctaac ttgcaaacaa gtatccaagc tggtcaaagc ttgcttggaa
    81661 aagcagatgc aactgaagct gaattatcag cagcagagtc atctattcaa tcatttatta
    81721 ttggtctaga acttcgttct aactctaata aagaaactgt atcagaaacg cctgtagcga
    81781 agaaagctga tgcagttgaa tcaaaagaag gggctaaacc agctgcaaca actgaacgtt
    81841 cagctgttga tagcgctatt ttgccaacta gcacagctga caaagtagaa acaacttcag
    81901 ctccagcatc tattaatgaa atcttgaaac taggtttgag cctttctgat gctcgccaaa
    81961 atccagctat ccgtaaggaa aatgttaata gagggtatag tggttttaga gcggctagca
    82021 atccagccaa cccaattgtc tcaggttctg gaaatacagt tgcatttgca gatattagcc
    82081 aaggtggtcg tagctatagt ttccgtggct acgggaactc acgtggtgga aattctattc
    82141 attacgatgt aacaacagta cgtagtggta atagtgttaa ctttacaatt agttattctg
    82201 cgccaggaga ttctagagag tttgttaata ataattttat cttggataaa ggggatggat
    82261 ttggaaatcc ttcaaatgca acgatcacaa gttcaaatcc aagagtaagg gagcaatcaa
    82321 aatctattag tcagggtgcc aactacgtat ctcactcagg atacagtatg acctctgcta
    82381 tttcaacaaa tacggaacaa actatcagat ttagtttgcc tatcattaat ctaaatggtg
    82441 atttgtctgt tcgtttgaaa cctgttactt ttaacgtgga ccaaggtggt ggtggtgccg
    82501 ctactagcaa tgacccatac agtaactcta actattatta tagagcaaac ccactatact
    82561 tggatgcaaa cccttatggt ggtactaata ataagactgt ttcagaagac attgacttcc
    82621 aaactgtcta tcttccaact agtaagttac cagaaggtca aactagatta gttcgagaag
    82681 gtgaaaaagg acaacgtcaa attacctata aagttcatcg atttggtaac gaaacacttt
    82741 taggattgcc gattagtaat agtgttacta aagaagctaa gccacgtatt atgcaaattg
    82801 gtgtggctaa agatctaatc gatacagtaa aaccacgtgt tgatcaaaat aaagtcggtg
    82861 atacaaataa cctcactttc tatcttgata acgatggaaa cggtgtttat actgaaggtg
    82921 tagacgaact tgttcaaaaa attgctatta aagatggagc taaaggtgaa aaaggagacc
    82981 aaggtgaacg cggtctaact ggagctaaag gtgaaaaagg agaccgaggc gaacgcggtc
    83041 taactggagc tcaaggagct aaaggtgaaa aaggagaccg aggcgaacgc ggtctaactg
    83101 gagctcaagg agctaaaggt gaaaaaggag cccaaggtga acgcggttta actggagcta
    83161 aaggtgaaaa aggggaccaa ggtgaacgcg gtctcactgg agcccaaggt gaaaaaggag
    83221 accgaggcga acgtggttta actggagcta aaggtgaaaa aggagcccaa ggtcaggcag
    83281 gtcgtgacgg tgtaactcca accgtaaccg ttaaagataa taaaaatgac ggcactcata
    83341 ctatcactat taacgacggt agaggtaatg ttacaagtac tgttgtaaga gatggtttcg
    83401 atggcgcaag tccattagtt gcgactcaac gaaatgacgc agataagaca acaactgtta
    83461 tcttctatta cgataaaaat ggaaacaatg aattagatgc ttctgataag aaattaaaag
    83521 aagttgtaat tgcagatggt gctaaaggtg aaaaagggga caaaggtgaa caaggtcttc
    83581 aagggcgtga tggtgaacaa ggaccaaaag gagaagatgg gaaaacccca acagttaaag
    83641 taactgatgg tcaagatgga acgcatacaa ttacaattaa cgatggtaaa ggtggtataa
    83701 ctactacagt agtaagagat ggatttgatg gtgcaagccc tcttgtttct actcatagaa
    83761 atgaagcaga taaaacaaca actgttattt tctattatga tctaaatgat aataatcaat
    83821 ttgatgaagg agatacaaaa cttaaagaag ttgttatcgc agatggaaaa caaggaccaa
    83881 aaggtgacaa aggtgataac ggaaaagatg gattcacacc agaagtaaca gttacagata
    83941 acaataacgg aacacacacc atcacaatca cacaaccaga caacagacca tcattaacaa
    84001 caatcgttaa aaacggtgaa gatggaaaaa caccaaaagt caaagcagaa cgagatgatg
    84061 cgaagaaaca aacaacatta acattctaca ttgacaaaga tggagatgga agttacacag
    84121 caggaaaaga cgagttagtt caaacaacag tagttaaaga cggacaagac ggagctgcag
    84181 gagcatctgg acgtgatgga aaagaagtat taaacggaaa agtagaccca acaacagaag
    84241 gtaaagatgg agacacattc gtaaatacac aaacaggaga tgtattcgtt aagaaaggta
    84301 acacttggga accagcagga aacatcaaag gaccgaaagg tgacaaaggt gcagatggtg
    84361 ccaagggtga aaaaggagcc caaggagaac gcggcctgac tggagcgcaa ggtgtcaagg
    84421 gtgaaaaggg agatcagggt gaacgcggtc taactggatc taaaggtgaa aaaggagacc
    84481 aaggtgaacg cggtttaact ggagcgcaag gtgccaaagg tgacaaaggt gaacaaggtc
    84541 ttcaaggtcg tgatggagct caaggaccaa aaggcgcaga tggacaaaga ggaccagcgg
    84601 gaccacaagg accaaaagga ccaaaaggag aacaaggtaa tccaggaact ccaggtaaag
    84661 atggaaaatc tctaattgct gttaaaaatg gaactgaaac aaaagtctat gtagaggatc
    84721 cggctagacc aggacaacca cttaatccaa accaaccatt agcaactatc actgatggaa
    84781 gaaacggtac taatggacaa tcaccaacaa ttacagcgac acgaagtgtg caaaatggca
    84841 aaaatggtgt attagtaaca attacaccag taggaggtcg tccacaaaca acatttgtag
    84901 aggatggaca aaagggtgct gatgggaaaa ccccaacagt aacaataact gaggggcaaa
    84961 acggcacaca tacattaacg gttcataatc caggaagtcc agatgtgaca actacgatcc
    85021 gtgatggagc tacaggacaa gcaggtcgtg atggtaaaga tgtattaaac ggaaaagtaa
    85081 atccacaacc aaaccaaggt aaaaatggag ataaatatat taataccgaa accggtgatg
    85141 tctatgttaa aaacaatgga aactgggata aagaaggcaa catcaaaggc cctaaagggg
    85201 acaaaggtgc agatggtgct aaaggcgaaa aaggagacca aggcgaacgc ggcctaactg
    85261 gagcgcaagg agctaaaggt gcggatggcg cagtaggtcg tgatggacgt gacggtaaag
    85321 acgtgttgaa cggcaaagct aacccagaag cacatcaagg taaagacggc gataaatacg
    85381 ttaatacaga aacaggcgac gtcttcgtta agaataacgg caactgggat aaagagggca
    85441 acatcaaagg ccctaagggt gacaaaggtg aacgcggaga agatggtaag actccagaag
    85501 taactgtaac tccaggtaaa gatggccata gtactgacat tacattcact gttccaggta
    85561 aagatccagt tacagtaaat gttaaggacg gagaaaatgg tctgaacggt aaaactccaa
    85621 aagttgattt acttcgtgtc caaggaaaaa acggaaatcc atctcataca attgtgacat
    85681 tctatacaga tgaaaacaat gacggcaaat atacaccagg aactgatgaa cttctaggtt
    85741 cagaaatgat taaagatggt gctaaaggcg cggacggacg agatggtaaa tcattgctta
    85801 ctgtcaagga tggtaaagaa actaaagttt accaagaaga tccagctaac ccaggacaac
    85861 cattaaatcc agaaaaacca cttgcggtaa ttagagatgg agtagatgga aaatcaccta
    85921 cagttacagc tgttcgtaaa gatgaagcag ggcataaagg tgtagaaatc actgttgata
    85981 accatgatgg ttcacaacca actacagtct ttgttcaaga tggtgctaag ggagaaactg
    86041 gtgcaaccgg tcaggatgga caaactccta caatcactac tcaacgtgga caagatggcc
    86101 aaagcactgt tgtaactatc acaacatcag gtaaagatcc agtaaccttc actgtaaaag
    86161 atggtaagaa tggtaaagat ggccgtgcac cgaaaatcaa agtagaagat attacttcac
    86221 cttcaagaat tagacgcgat acagatgctg ctgcaactcc aacgcgtaac ggtatccgtg
    86281 ttacagttta tgatgatgtt aatgacaatg gggtatacga cgaaggtgtc gataaagtat
    86341 taaatagtaa agatatttat aacggtatag atggacgtga tggttcagct ccaactatta
    86401 ctacaaaaga taatggagat ggaactcaca ctatcacagt tcaaaaccca gatggttctg
    86461 aatcaacaac agttgttaaa gatggtaaag acggtaaaac tgcgaatatc actacaacag
    86521 aaaacccaga tggaagccac acaattacgg tgacaaatcc agatggttca actaaagaaa
    86581 ctgttgttaa aaacggtaaa gacggtaaga ctcctaaagt tgaagtaacg gataacaacg
    86641 atggaactca tactgttaaa gttacagatg gagacggcaa tgttaccaac gctatcatca
    86701 aagatggtaa agacggtaaa gctgcaacag caacaactac tgaaaatcca gatggaagcc
    86761 acacagtaac aatcactaac ccagacggaa ctaagaatga gtttgttgtt aagaatggac
    86821 gtgacggtgt tgacggacgt actccaaccg catctgttcg tgataatgga gacggaagtc
    86881 atacaatcgt tattacaaat ccagaaggtg tgacaactga aaccacagtt cgtgatggta
    86941 aatcaccaaa agtgactata actgatgaac aaaatggaac tcataagatc tctgttctaa
    87001 atggtgacgg aacaactaca acaactacag ttcgtgatgg tagagaacca aaacttgaag
    87061 ttattgataa caacgatggt tcacacacta ttaaagtgac aggtgctgat ggtaaaggaa
    87121 cgacaactac aatctttgat ggtaaatcac caaaagcgaa catcgttgat aacggagatg
    87181 gaactcatac attaacaatc gtagattctg atggtcgtga atacaaatct attatcaaag
    87241 atggtaaaga cggcaaagat agcgtttcac caactgtaac tgttaaaaat aataacgatg
    87301 gaactcacgt tgttacaatc actaatccag atggaagtaa gacagaaatg gtgattaaag
    87361 acggtaaaga tggtaaatca ccaaaagttt ctgttgaaga taatggtgat ggtagtcata
    87421 caatcacaat catcaattct gatggaactg tgacaaaaac agttattaaa gatggtagag
    87481 atggacgtga tggtcgagac ggcaaagacg gtaaagacgg taaagatgga aaatgtggat
    87541 gccaagacaa accagtaaca ccatcaaatg acaaaccagt tcctccaaca ccaaatgtgc
    87601 cgacaccaga agtaccggtt aaacctgtgc cagcgcaacc aacaccaaat gtaccgacac
    87661 cagaagtgcc agtacaacca actccagctg ttccaacacc agaagtaccg gttaaaccag
    87721 taccagcggt tccagaacaa ccagtagtac caacaccggc tcaaccagca actccagtaa
    87781 atgctaaccc agtagcacca actacaggta aagaaaaccg tggggacaaa ttacctgaaa
    87841 ctggaagcca atctgattat atctctgttc ttttaggtag cggtattcta ttgagcctat
    87901 atgtaggacg aagaaaagaa gcttaatcag ccaaaagcag tcactaattt ctcgaaagaa
    87961 gatgataagt gatagatctc gaatgaaaga atattcttag aatgattttc ctatgatgga
    88021 aaagactgct aaaaaccttg agtggtaacg ctcaaggttt ttttggatgg ctgatgtttg
    88081 tctgaaaatc ttgattgaga aataaaaagc caccctatta ggtggctata attgatttag
    88141 actttctaaa attttctatt ttttactgaa tcgcttcgac caagtcttga gcttgttttt
    88201 caagtgagtt taggactgtt tcttcaagaa ccaattttcc gtctgcccag gcagagtcat
    88261 taacacgtgc agcagtgaaa tcaccaacgc cttgtgtacg gataaatggc aagaggtctt
    88321 tgtagatagc gaaaagttga tcgtgccctg cattggctac agatgatacg gtgacaaact
    88381 tgtcttgaag ggcagaaacg ccacgcgtat cagacaagtc aagggcacga gatagccagt
    88441 caagcaagtt ttttactgta ccagggatag agaagttgta gactggagag aaaatccaga
    88501 tagcatccgc aacgagaact gcttcacgag cagcagctac agctggatgt gttggaactt
    88561 ccaaatcttg gctgaagaga ggaagggctg aataatcaag gtagctaact tccgctttac
    88621 cagcaagtgc tttctcagct tcgagcgcca tttggtggtt gaaagaacct tgacgtagtg
    88681 atccgacgat aaataatact tttttagaca tgatgtgtct cctttttctt caaattttga
    88741 gaactctctc gttgttataa aatacattac aacaaaacaa aagaacttgc aagaaatttg
    88801 ctcagagttt atttgaaaat tgtaaaattt cggaaactct agtcatttgt gactgaaaaa
    88861 gtaagaactg tttcactttc aaaatcggtt ttgtttgaag ggagaaatag aattggttgc
    88921 tcaaagcaca gctttgaggt tgcagataaa gctgacgtgg tttgaagaga ttttcgaaga
    88981 gtattcttat attcacttgt aataaagctt aatagatggt aaaatagacg agtgaaaaaa
    89041 agacaaaaca aagcaaaaaa taatctactg tggcagtatg gtctagggat gacgattttg
    89101 tttgtggtta tcagtgcttc ctttctgtat atggtttctc ttagcatgaa accctatcaa
    89161 acagctaaaa gtgaaggaga aaaattagct cagcagtatg caggattaga gcaggccgat
    89221 caggttgatt tatacaatgg cttggaatct tattacagcg ttcttggtcg taataaacag
    89281 caagaagcac ttgctgttct gattggaaaa gatgatcata agatttacgt ttatcagcta
    89341 aatcagggtg tttcacaaga aaaagcagaa acggtttcta aggaaaaggg agctggcgaa
    89401 attgacaaga ttatctttgg tcgttatcaa gataagccaa tctgggaagt caagtcagga
    89461 tctgattttt atctagtaga ttttgaaaca ggagcattgg tcaacaagga gggcctatga
    89521 aattatccaa ccgtgtttta gaaatggaag aaagtgtgac tttggctgct ggagcccgtg
    89581 ccaaagcact gaaggctgag ggtagagata ttttgtctct aaccttgggt gagccagatt
    89641 tcactactcc caaaaatatc caagatgccg ccattgcatc gattcgagat gggcgtgctt
    89701 ctttttatac agtaacctca ggtctgccag agcttaaggc ggcggtcaat agctactttg
    89761 agcgctttta cggctattct gtagcgtcaa atcaagtgac agtcgctgcg ggagccaaat
    89821 attctctcta taccttcttt atggctgtgg tcaatccagg tgatgaagtc atcatcccaa
    89881 ccccatactg ggttagctat ggagatcagg tcaagatggc agaaggcgtt cccgtctttg
    89941 tttctgctaa ggaagacaat cactttaagg tgaccgtaga gcagttagaa gcagctcgca
    90001 ctgacaagac caaggttttg gtgctgaatt cgccatctaa tcccacaggt atgatttaca
    90061 cccgtgagga actcttggca attggaaact gggctgtaga aaatgatatt ctcatcctag
    90121 cagacgatat ctatggccgc ttggtttata atggtcatga gttcacaccg atttctagcc
    90181 tatcggaagc gattcgcaag caaacagtgg tcatcaatgg tgtgtctaaa acctatgcca
    90241 tgactggttg gcggattggt tatgccgttg gagaagcaga cattatcgct gccatgtcca
    90301 agattgcagg tcaaacaact tcgaatccgt cagcagtagc ccaatatgca gcagttgagg
    90361 ctctatcagg tgagcaagat actgtagaaa gcatgcgtca ggcctttgag gaacgtctta
    90421 ataccatcta tcccctcctt gcagaggtgc caggatttga agtggtcaaa ccgcaagggg
    90481 ccttctatct cttcccaaat gtcaaaaagg ccatggagat gaaaggctac acggatgtga
    90541 cagactttac aactgctatc ttagaagaag ccgaagtggc cttggtaaca ggagctggat
    90601 ttggagcacc agaaaatgtc cgcctcagct atgcgacaga cctagacacg cttaaagaag
    90661 cagtcgaacg cttgaaagca tttatgggta gtgagaatga ttgatcattt tgagattaag
    90721 gtaaaggatt tacaaatttc agaaggattt tataggagtt ttctcgctcc tttggactat
    90781 aaattggctt ttaagactag ttctctaatt agttttctat ccccgaacag ccctcatcct
    90841 ggtggtgatt tttggctgac tcagggaaca caagatcctg ttcattttgc ttttttagca
    90901 gaaaataagg aagaagttta ggcttgttat gaggctggct tagaggcagg tgggcgagat
    90961 aatggggctc ctggttatcg aagtgagcgt ccgatttact atgctgcttt tatgattgac
    91021 ctggatggga acaatataga ggtggtttgc cataaagaat aaaaaagaaa aggaaaaata
    91081 atgacaaaac gtgtaacgat tattgacgta aaagactatg ttggtcagga agtgacgatt
    91141 ggcgcttggg ttgccaataa atcaggaaaa gggaaaatcg ctttcttaca attgcgtgat
    91201 ggaacagcct tctttcaagg tgtggctttt aaaccaaact ttgtcgaaaa atttggtgaa
    91261 gaagtgggac ttgagaagtt tgatgttatc aaacgcttga gccaagaaac gtctgtttat
    91321 gtgacaggta ttgtcaaaga ggacgaacgt tctaaatttg gctatgagtt ggacatcaca
    91381 gacatcgaag tgatcggtga atctcaagac tacccaatca caccaaaaga acacggaaca
    91441 gactttttga tggataaccg tcacttgtgg ctacgctctc gtaagcaagt agctgtgttg
    91501 caaatccgta acgctattat ctatgcaact tatgagttct ttgacaagaa cggttttatg
    91561 aagtttgaca gcccaattct ttcaggaaat gcggcagaag attctacaga actctttgaa
    91621 actgactact tcggaacgcc agcctacttg agccaatcag gtcagcttta cctagaagca
    91681 ggggctatgg ctcttggtcg tgtctttgac tttggtccag ttttccgtgc tgaaaaatca
    91741 aaaacacgcc gtcacttgac tgagttctgg atgatggatg ctgagtactc atacttgaca
    91801 catgatgagt cgcttgactt gcaagaagct tatgtgaaag ctcttctaca aggtgttctt
    91861 gaccgcgcgc ctcaagcctt ggaaaccttg gaacgtgata cagaactctt gaaacgctac
    91921 attgcagagc cattcaaacg tatcacttac gatcaagcca ttgacctctt gcaagagcat
    91981 gaaaatgatg aagatgctga ctacgagcat cttgagcatg gtgatgactt tgggtcacca
    92041 cacgaaactt ggatttcaaa ccactttggt gtgccaacat ttgtcatgaa ctatccagta
    92101 gccatcaagg ccttctacat gaaaccagtt cctggaaatc cagagcgcgt gctttgtgca
    92161 gacttgcttg ctccagaagg ctatggagaa attatcggtg ggtctatgcg tgaggaagat
    92221 tacgatgccc ttgtcgctaa gatggatgaa cttggcatgg atcgtacaga atatgaattc
    92281 taccttgacc ttcgtaaata cggtacagtt ccacacggag gatttggtat cggtatcgaa
    92341 cgtatggtaa cctttgcagc aggaacaaaa catatccgtg aagctattcc attcccacgt
    92401 atgttgcacc gtatcaaacc ataagaacag gaaaacgccc atgtggcgtt ttttcgaatt
    92461 ctagtgtttt tacttgcgta aaaaaatttt ttctagtata atagtttatt gtgagcgaac
    92521 ctcacttacc ccttgcaaag tcttggggtc attagaccaa aaggaggaac atatcaatgg
    92581 ctaaatacga aattctttat atcattcgtc caaacattga agaagaagct aaaaacgctt
    92641 tggtagcacg ttttgactct attttgactg acaacggtgc aactgttgtt gaatcaaaaa
    92701 cttgggaaaa acgtcgtctt gcatacgaaa tccaagattt ccgtgaagga ctttaccaca
    92761 tcgttaacgt tgaagcaaat gacgatgcag ctcttaaaga gtttgaccgt ctttcaaaaa
    92821 tcaacgctga cattcttcgt cacatgatcg tcaaaattga cgcgtaagaa ggtaaactat
    92881 gattaacaat gttgtacttg tagggcgtat gacacgtgac gctgagttgc gttatacccc
    92941 atcaaatgta gcagttgcga cttttactct tgcagtaaac cgtacattta agagtcaaaa
    93001 tggtgaacgt gaggctgatt ttatcaatgt cgttatgtgg cgccaacagg ctgaaaatct
    93061 tgctaactgg gctaaaaaag gctcacttat cggggtgaca ggtcgtatcc agactcgtag
    93121 ttacgataac cagcaaggac aacgtgtcta cgtgacagag gtcgtggctg agaatttcca
    93181 aatgttggaa agccgtagtg tgcgtgaggg tcacacaggt ggagcttact ctgcaccaac
    93241 tgcaaactat tcagcaccta caaattcagt actagacttt tcacgtaatg aaaatccatt
    93301 tggagcaaca aacccattgg atatttcaga tgatgattta ccattctaat ggacaactaa
    93361 acttataaag gagaaaaaac atggctcaac aacgtcgtgg cggattcaaa cgccgtaaaa
    93421 aagttgatta catcgcagca aacaaaattg aatatgttga ttacaaagat actgagcttc
    93481 ttagccgttt cgtttcagaa cgtgggaaaa tccttcctcg tcgtgtaaca ggaacttcag
    93541 ctaaaaatca acgtaaagta acaacagcta tcaaacgcgc tcgcgtaatg gctttgatgc
    93601 ctttcgtaaa cgaagattaa agaaaagacc cggttgggtc tctttttcat gagcttgaaa
    93661 acaagaaagc gaattaagac cccaatgggt cttttttcac aaactttgaa atgagagagc
    93721 gagttaggct cagtggctcc ctttttacga gtttgaaaac aagagaacac attaaaagca
    93781 cgatggagct ttttcataaa tatgtctttt ttctcatcca agaaattaaa ataaaaatga
    93841 taaacttttt gaaaataggg gatttgccta ctttatgata taatggaaag agttagattg
    93901 aaagaggtag tgagatggac gcgaaattaa gatacaaggc aaagaagatc aagattgtct
    93961 tttttgatat tgatgataca ttgcggaatt caaagacagg gtttattcca actacaattc
    94021 ccactgtttt taaacagttg cgtgaaaaag gaattttaac aggaatcgcc tctggacgtg
    94081 gcatttttgg tgttgttcca gagattcgtg atctcaagcc tgactttttt gtaactttga
    94141 atggggctta tatcgaagat aaaaaaggtc aggtcattta tcagcatcag attgaaaagt
    94201 cagatgttga ggagtatatc tcttgggcta agcaagaagg aattgagtat ggcttggttg
    94261 ggagtcatga tgccaagttg tcgactcgca ccgatatgat gagtgaagct atcaatccaa
    94321 tttatcccga cttagatgta gatcccgatt tccatgaaaa agaagatatc tatcagatgt
    94381 ggacttttga agataaggga gatgacttgc acttgcctga cagtctctca gacaaacttc
    94441 gcatggttcg ttggcatcaa cattcgtctg atattgtgcc gatttcaggc tccaaagcga
    94501 cgggggtgga aaaagttgtg gaacaccttg gcttgaaacc agagaaggtc atggtttttg
    94561 gagatggtct caacgacttg gaactctttg attatgctgg aatcagcgtt gccatgggaa
    94621 tttctcatga taaaatcaaa gaaaaagcag attatattac aaaaacatta gaagaagatg
    94681 gcatttttga tgccttagaa gtatttggta tggtagaaaa agaattgcat tttccacaag
    94741 tagacattga aacagtagaa ggtcctcttg cgactattaa gaccaatcac ggagacttac
    94801 gtatcaagct tttccctgaa catgctccta aaacagtggc taactttgta tctctttcaa
    94861 aagatggcta ctatgatggt gtcattttcc accgtattat caaggacttt atgatccaag
    94921 gtggaaaccc aactggaact ggtatgggtg gcgagtcaat ctacggcgaa tcttttgagg
    94981 atgaattctc agaagagctt tacaatatcc gtggtgctct ttccatggca aatgctggtc
    95041 caaataccaa cggcagccag ttctttatcg tgcaaaacca acacctacct tattctaaga
    95101 aagaaattac tcgtggtggt tggccagaac cgattgcaga aatctatgcc aatcaaggtg
    95161 ggacacctca cctagaccgt cgtcacacgg tttttggtca gttagctgat gaagcatctt
    95221 acgctgtctt ggatgccatt gctgctgttg agacaggagc tatggacaag ccagttgaag
    95281 atgttgtaat tgaaactatt gaaatcgagg actaagatga aaatcggtga taagctaaag
    95341 gggcgtatta cagggattca gccctacggt gcctttgttg agttagagac gggtgatacg
    95401 gggctgattc acatttcaga aattcggaca ggatttattg aaaatattca tgaaaccttg
    95461 aaagtcgatg aagaggttca agttcaggta gtggatttag atgaatttac ggggaaagcc
    95521 agtctttcta tccgcacttt ggaggaagaa aagtaccagt ttcctagacg gagacgcttt
    95581 tcaagcgacc gctttaacta cggttttgct ccctttcgac gaatgctacc aatctggact
    95641 ggagaagccc tgcaccattt aaaaaagaag aagtagttta gagttttata ttttttgtaa
    95701 tgttaatata aatttgctat aataggacca tggaagaaga acaattatta aaatcaggag
    95761 agcgcattaa ccagctcttt tcgacagata tcaaaatcat tcaaaataga gaggttttta
    95821 gctattcggt ggatagtgtt ctcttatcac gatttccacg ttttcctaag aaggggttga
    95881 ttgtggattt ctgtgctggg aatggagcag tggggctttt tgctagcact cgtactcaag
    95941 cacagatatt gtctgttgag attcaggagc gtttggcgga tatggctgaa cgctctgtcc
    96001 gtttgaatgg attagaggag caaatgcagg tcatctgcga tgatttgaaa aatatgcctg
    96061 ctcacattca ggggagtaag gtggatatga ttttgtgtaa tccaccctat ttcaaggtgg
    96121 atccttattc taatctgaac gagagtgaac attatctctt ggctcgacac gaaatcacga
    96181 ctaatttgga agaaatctgt catagtgccc agagtattct caagtctaat gggcgtttgg
    96241 ccatggttca tcgtcctgat cgacttctgg atattttgga tacgttaaaa cgacataatc
    96301 tagcccctaa gcgcctgcag tttgtttatc caaaaagaga aaaggaagcc aatatgcttt
    96361 tgattgaggc gatcaaggat ggctcaacaa gtggctttaa ggtcttacct cctttgattg
    96421 tccacaatga tgatggctct tatacgcccg aaatcgaaga gatttattat ggatcataag
    96481 gcttatatgt acgttctgga gtgtcgtgat ggatcctact atataggcta tacgactgat
    96541 atgagaagac gcctcgctat ccacaatagt gggaagggag ccaaatatac acgagcacgc
    96601 ttgccagtca aacttatcta tgctcaaggt tttgccagta aggaagaagc catgtcggct
    96661 gaagctcttc tcaagcgtaa gaagaggcca cagaaggaag aatttttatc tgaaaatcaa
    96721 gatagaaatt tactcagtta ttttgaagaa tcgtggggag tcctttgact cctcttacct
    96781 ttgtcaaaaa gcgaaaaaaa tgctacaata aagaaaataa acaaaatgag gtattatcat
    96841 gtccaagatt ctagtatttg gtcaccaaaa tccagactca gatgccatcg gatcatctgt
    96901 agcttttgcc taccttgcaa aagaagctta cggtttggat acggaagctg ttgcccttgg
    96961 aactccaaat gaagaaacag cctttgtctt gaactatttt ggtgtggaag caccacgtgt
    97021 tatcacttct gccaaagcag agggggcaga gcaagttatc ttgactgacc acaatgaatt
    97081 ccaacaatct gtatcagata tcgctgaagt agaagtttac ggtgttgtag accaccaccg
    97141 tgtggctaac tttgaaactg caagcccact ttacatgcgt ttggagccag ttggatcagc
    97201 gtcttcaatc gtttaccgta tgttcaaaga acatggtgta gctgtgccta aagagattgc
    97261 aggtttgatg ctttcaggtt tgatttcaga tacccttctt ttgaaatcac caacaacaca
    97321 cccaacagat aaaatcattg ctcctgaatt ggctgaattg gctggtgtga acttggaaga
    97381 atatggtttg gcaatgttga aagctggtac caacttggct agcaaatctg ctgaagaatt
    97441 gattgacatc gatgctaaga cttttgaact caacggaaat aatgtccgtg ttgcccaagt
    97501 gaacacagtt gacatcgctg aagttttgga acgccaagca gaaattgaag ctgcaatgca
    97561 agctgccaac gaatcaaacg gctactctga ctttgtcttg atgattacag atatcgtcaa
    97621 ctcaaactca gaaatattgg ctcttggtgc caatatggac aaggtcgaag cggctttcaa
    97681 tttcaaactt gaaaacaatc atgccttcct tgctggtgcc gtttcacgta agaaacaagt
    97741 ggtacctcaa ttaactgaaa gctttaatgc gtaagatttt gggtgtcagc tcaaaatcgg
    97801 aaagtctagt ttgccttata tcgaaaggag tttcggctcc tttttttcta ggagtgaagt
    97861 atgttagaaa atggcgattt gatttttgtg agagatgggt cagacatggg acaggccatc
    97921 cagacttcca caggtaacta tagccatgtt gccatttatt tggatgggat gatttatcat
    97981 gctagtggac aggctggtgt tgtctgtcaa gaaccggcag acttctttga gtccaatcat
    98041 ttgtacgacc tctatgttta cccagaaatg gatatccagt cggtgaagga aagagcttgc
    98101 agacatcttg gagcacccta caatgcttct ttctatccag atgcagctgg tttctactgc
    98161 tcccagtata tagcagaaat cctacctatt tttgaaacta ttcctatgaa atttggagat
    98221 gaggaacagg agattagtga tttttggagg gagtattaca tagaactagg tctgcctgtt
    98281 cctctgaacc aagctgggac caatcctagt cagttggcag catcgcctct gttacaatgt
    98341 aaagaaagga atcttcatga ttcagatttt taatccatct cgtttgacga gacagccatt
    98401 tttggagaat tgatccgcta tctggaccag tatgaggatg tgattctacg ggaaattaag
    98461 gctcaatttc cagatgttgc agttgataaa ctcatggaag agtatataaa ggcaagcttg
    98521 attctacgtg aaaataagcg ctattacctt aattttccta cgcttgaatc acttgatagt
    98581 cttgaactgg atcaagagat ttttgtcaga gaagctagtc cggtctatca agccttgttg
    98641 gagcagagtt ttgagacgga attgcgcaat caaatcaatg cagctatttt agttgaaaag
    98701 acggactttg cgcgcattaa aatgaccctg tccaattatt tttacaaggt caaacagcag
    98761 tatcctttga cagaaaaaca gcaggagctc tatgacattt taggagatgt taatcctgag
    98821 tatgccctca agtatatgac ggcttttttg ttgaaatttc tcaaaaaaga ccagcttatg
    98881 cagaaatgcc gtgatatctt tgtggacagt ttggttgtct taggctatat tgtgcaaaat
    98941 gaagatggaa agtatgagtt ggctatcgat tttgataagg agagattaac tttctactta
    99001 gcgtgatttc ttgtttctga gtacattgtt tgactttcct tagtattcgg tataaactat
    99061 atgtaaccgg taacacatat cggaataaac taaaggagac aatcatatgt cacttgaaaa
    99121 caaattggaa caagcaacag gcgctgtcaa agaaggtttt ggtaaagtta ctggagacag
    99181 caagacagaa cttgaaggag ctgttgaaaa aacagttgct aaggcaaaag acgttgtaga
    99241 agacgcaaaa ggtgctgtag aaggtttgaa aaacgttttt actaaataat aggaaaaaat
    99301 caagggtttc attttccctt gattttttct attcttataa ataattttct gcgacggctg
    99361 tatctcctgg gtaggattct ttcttgccct ggatgatttg gtaacaatcg actcccttac
    99421 ccgcaataat aactgcatct aattcgtgat ttgtgatagc cattgccgcc ttgatggctt
    99481 cttggcgatc cgcaatcttt tcaacaggat gattgatgta gctactaatt tcatctgcaa
    99541 tggccattgg gtcttcatag ttagggtcat cagcagtcag aaagacttga atctcagggt
    99601 gttgattgag gaggaggcca aagtccttac gacgactttc tcccttgttt cctgttgatc
    99661 ccagaaccag agcaatcttt ccggtttgat gagtttccac cacattgatg agttttttca
    99721 gactatcccc attgtgggca tagtcgatga agaccttggc tccatttttc tgagtgagga
    99781 cttccatacg accaggaacg cgggttgcag cgatgccttt tttgatgtcc tcaagacttg
    99841 ctccgagacg gagacaagca agtccagcag caactgcatt ttcttggttg aagttgccaa
    99901 tgagttggat atcataatct ccagcgagtt tacccgtagc tgaaaagcta aaggctttgg
    99961 aattctcgat ttggttatca aattggctac catagaaatc atggtcttga tcttcaacct
   100021 gttctttcaa gactgagaag tggtccatgt cactgttaat gatgactgct cggctatttt
   100081 ccatcaagag acgcttgtgg tagaaatagt cttcaaagct agggtgttca atcgggccga
   100141 tatggtctgg gctgatattg aggaaaactc ccacatcaaa ggttagacca tagacacgtt
   100201 tgaccagata ggattgactg gagacttcca tgatgaggtg ggtacggtca ttttgcacag
   100261 cctgattcat catgtcaaag aggtcaatac tctcaggggt tgtcaacgct gacttaaaga
   100321 aagtctcgcc atcaagagtt gtgttcatgg tcgacaacat agcaggtcta tgcccttgag
   100381 ataagatgtt ataggcgaaa taggttgctg ttgtcttacc cttagtacca gtaaaggcaa
   100441 ggagtttgag tttctcttgt ggattaccat agaactccat ggcaatcaaa ctcatggctt
   100501 tctttatatc gttcacaatg atgacaggga tatcgacttc gtagtccttt tcagctacat
   100561 accaagctaa accttgtgtt atagcagaaa gaaggtattc ttttttaaag gcagcgcctt
   100621 ttgcaaaaaa aagagtgtct tctgttactt ttcggctgtc gtagctgatg ctatcaaaaa
   100681 caactttgct gtagttgtag tggtaatgac cttggtcaat aatttcgcga aaaaggccat
   100741 ctttctttaa aatatctaat acggtttcaa tcttaatcat actttctatt gtaaaccgaa
   100801 agtcgcaaat ttacaagtaa caaggaaaag tttataatgg aagataagga gtttttccta
   100861 gttatcaaaa ttgaatgagg aatctatgtc gcacgaaaac aatcaccagc aggcccagat
   100921 gttacggggg actgcttggc taacggctag taactttatc agtcgcctac tcggggctgt
   100981 ttacattatc ccttggtaca tctggatggg ggcttatgca gctaaggcaa atggtctctt
   101041 taccatgggt tacaatatct atgcttggtt cttgttggtt tcaacagcgg ggattccagt
   101101 tgcggtggcc aagcaagttg ccaagtataa taccatgcga gaagaagagc atagctttgc
   101161 cctgattcgg agcttcttag gctttatgac aggactaggc ctggtttttg ctttagtctt
   101221 gtatgtcttt gctccttggc tagcagactt gtctggcgtg ggcaaagact tgatcccaat
   101281 catgcaaagc ttggcttggg gagtcttgat tttcccgtct atgagtgtta tccgaggatt
   101341 tttccaaggg atgaataacc tcaaacccta tgccatgagc caaattgctg agcaggtcat
   101401 tcgtgttatc tggatgctcc tagcaacctt tatcattatg aagctcggtt caggagatta
   101461 tctagcagcc gttacccaat caacctttgc tgcctttgtc ggtatggtag ccagttttgc
   101521 agtcttgatt tatttccttg cccaagaagg ttcactcaaa agagtctttg aaacaggaga
   101581 taagattaac agtaagcgtc tcttggttga taccattaag gaagccattc cttttatcct
   101641 gacagggtct gccatccagc tcttccagat tttggatcag ctgaccttta tcaatagtat
   101701 gagctggttt accaactaca gcaatgagga cttggttgtc atgttttctt atttctcagc
   101761 caatcctaat aaaatcacga tgattttgat ttctgtaggg gtttcgattg ggagtgttgg
   101821 tttgccactt ttgacggaaa actatgtcaa gggggacttg aaagcagctt ctcgtctggt
   101881 tcaggacagt ctcaccctac tctttatgtt cttgctacca gcaacggttg gagtggttat
   101941 ggtaggagaa cctctttata cggtcttcta tggtaagcca gatagtttgg ctctgggctt
   102001 atttgtcttt gcagttttgc agtctattat tttaggcttg tacatggtct tgtctccaat
   102061 gcttcaggcc atgttccgca accgcaaggc cgttctctat tttatctatg gttctattgc
   102121 caagctagtc ttgcaactac ctaccatcgc cctcttccac agttatggtc ctttgatttc
   102181 aacaaccatt gctctcatca ttcctaacgt cttgatgtat cgggatattt gtaaagtaac
   102241 tggtgtcaag cgcaaggtga ttttgaagcg aaccatttta atcagtttgc tgaccctagt
   102301 catgtttctg ttaataggaa ccatccagtg gctgttagga tttttcttcc aaccaagtgg
   102361 acgtttgtgg agcttctttt atgtagctct tgtcggtgcc atggggggtg gactttatat
   102421 ggttatgagt ctgcgtacct atttattaga taaggtaata ggaaaagccc aagcagatcg
   102481 cctgcgagca aaatttaagc tttcgtaagg tagcatttat aaataaaagg aataagttga
   102541 acaatctttt aggagaactt gaaacttatt ccttttttag cgtaaaaata gctaaaatta
   102601 tctttcaaaa atagaaaatg aggttttttt cgattttttg gaaaaataat tgactaaaca
   102661 aaccaaacgt tgtataataa aacaaatatt taaagcagga ggttctggaa atgaaaaagt
   102721 ctaagagcaa atatctaacc ttggcaggtc ttgtcctggg tacaggagtt ttattgagcg
   102781 cgtgtggaaa ttctagcacg gcgtcaaaaa cctacaacta tgtttattca agtgatccat
   102841 ctagcttgaa ctatctagca gaaaaccgcg cagcaacatc cgatattgtt gcaaatttgg
   102901 tagacgggtt attagaaaat gaccaatatg ggaatattat tccatcatta gcagaggatt
   102961 ggactgtttc tcaggacggt ttgacctata cctacaaact tcgtaaggat gccaagtggt
   103021 ttacttctga gggagaagaa tatgcgcctg taactgccca ggattttgtg acaggtttgc
   103081 aatatgcagc tgataaaaaa tcagaagcct tgtatctagt gcaggactct gttgctggtt
   103141 tggatgacta tatcactggt aaaacaagcg acttttcaac tgtcggtgtc aaggcacttg
   103201 atgaccaaac ggttcaatat actttggtta aaccagaact ttactggaat tcaaaaacac
   103261 ttgcaacgat actttttcct gttaatgcag atttcctgaa atcaaaaggg gatgattttg
   103321 ggaaggcgga tccatctagt attttgtaca atggaccttt cttgatgaaa gcacttgtct
   103381 caaaatctgc tattgaatat aagaaaaacc ctaattactg ggatgctaag aatgtctttg
   103441 tagacgatgt gaaattgacc tactatgatg gtagcgatca agaatcactg gaacgtaatt
   103501 ttacagctgg tgcttatact acggctcgtc tttttcctaa cagctccagc tatgaaggga
   103561 ttaaagaaaa atacaaaaac aatatcatct atagtatgca aaattcaact tcatatttct
   103621 ttaattttaa cctagatagg aagtcttaca attatacttc taaaacaagt gacattgaaa
   103681 agaaatcgac tcaggaagca gttctcaata aaaacttccg tcaggctatc aattttgctt
   103741 ttgacagaac atcttatggg gctcagtctg aagggaaaga aggtgcaaca aagattttgc
   103801 gtaacctagt ggttcctcca aactttgtca gtatcaaggg aaaagacttt ggtgaagttg
   103861 tagcctctaa gatggtcaac tatggtaagg aatggcaagg tatcaacttt gcggatggtc
   103921 aagaccctta ctacaatcct gagaaagcca aggctaagtt tgcggaagct aagaaagaac
   103981 tcgaagcaaa gggtgttcaa ttcccaatcc acttggataa gactgtggaa gtaacagata
   104041 aagtaggcat acaaggagtt agttctatca aacaatcaat tgaatctgtt ttaggttctg
   104101 ataatgtagt gattgacatt cagcaattaa catcagatga gtttgacagt tcaggctact
   104161 ttgctcaaac agctgctcag aaagactatg atttatatca tggcggttgg ggacctgatt
   104221 atcaagaccc gtcaacctat ctcgatattt ttaatactaa tagtggagga gttctacaaa
   104281 atcttggact agagcctgga gaagccaatg acaaggctaa ggaagttggg ctggatgtct
   104341 atactcaaat gttggaagaa gctaataaag agcaagatcc agccaaacgt tatgagaaat
   104401 atgctgatat tcaagcttgg ttgattgata gttctttagt tcttccaagt gtttcgcgtg
   104461 ggggaacacc atcattgaga agaaccgtac catttgctgc tgcctatggt ttaaccggta
   104521 caaaaggggt tgaatcatat aaatacctca aagtacaaga taagattgtc acaacagacg
   104581 aatatgcaaa agccagagaa aaatggttga aagaaaaaga agaatccaat aaaaaagccc
   104641 aagaagaatt ggcaaaacat gtcaaataaa atctaattgt agataagttt gtgtaagaag
   104701 tctttgtgct taaaaacgca taatatcagg tatataaaat cctagatgtt atgcgttttg
   104761 ttataggaag atttattaga ttttatcctc aaattgagtt attcccgact ttatccgttg
   104821 aaatattata aggagtattt atgaaattaa aattattaag agtagatact aaggtgatta
   104881 tggggagttt cttccttgtt ctgtctagtc tacttgcttt gttgcttccc cttatcttaa
   104941 aaggtttaat agatgggagt tctattgaaa atataggttc caaagtattt caatcgtttt
   105001 tgatttttat tggtcaagcc ttgttttctt ctattggtta ctatctgttt agtcaatcgg
   105061 gtgaaaaaag atagcaaaaa tcaggaaaaa agtgatagag gggttgattt atgcagagaa
   105121 atccttcttt gataagagcc aaagtgggga gttgacttct gccattgtca atgacacgag
   105181 tgtcattcgt gagtttttaa ttacgacttt cccaaatatt attctgagtt tagttatggt
   105241 acttggttcc attgtagtct tatttagtct tgattggaat ctttctctac ttttattcat
   105301 cactcttcct tgtatgatgt ttattatctt gcccctttcc aatatcagtg aaaagtatag
   105361 tcgtcgttta caggaggaaa tcggattttt aacagggcaa ttgactgaaa agattcaaga
   105421 acatgaatta attaagacta accaagcaga gaaaagtgta caggatgttt tggataattg
   105481 tattgaaagg gtacaaaaca attcactgaa atcagatagg attacttctt ttgagacccc
   105541 gtttgctctc ttatttatct ttgcgactat agctgtgatg ctaacctatg ggggttatcg
   105601 ggtcagcgca ggatatatat ctgtgggaac cttggtttcg tttttgattt acctctttca
   105661 attacttaat cctattagta atatagctaa ttttgtaact gtttattcta ggagcaaggg
   105721 atcttcagtt gcactggaga acttgcttgc agttcctaaa gaaaaatttg agggaggaaa
   105781 atcggtatca ggacgagggt tgaattttaa ccatgtctat tttggttatg atgaaaatcg
   105841 acctgtctta aaggatatta tttgttcaat tttcaagggg caaaaaattg cttttgttgg
   105901 accatctgga tcaggaaaat caacgattgt gcgtttgtta gagcggtttt ataaaccgct
   105961 ttcaggagat attctaatgg agcaatcaag tatatatgat tttaacttaa aagaatggag
   106021 aagtaaaatc gcttgggttt cacaaaataa tgcagtctta tctggcagta ttcgtgacaa
   106081 tctttgtctc ggtttgaatc gcttagtaac tgatgatgaa ttgatgaaag tgctagactt
   106141 agtatcacta ggtgatgaga ttcgctccat gaaagaggga ctagatactg aagttggtga
   106201 acgcggacga ctcttgtcag gggggcaaag ccaaagactt caaatagcta gagcctactt
   106261 aaaagatgct gaaattctta tatttgatga agccactgct aatcttgatg cggattctga
   106321 gtatgcgatt atcagtagcc tctattctgt attaaaggag aagacggttg tgattatagc
   106381 gcatcgtttg tcaacggtaa aagatgtgga ttgtattttc ttcttagagg aggggaaaat
   106441 cactggctca ggaactcata aggaactact ggaaaatcat gagcgttatg ctcgttttgt
   106501 gcaggagcaa atgatagagt gaaggtgtct tttgagattc accattttat agtctattaa
   106561 agggagcagg aaaaactccc tttttatata gtttgaaact ataactagct cttgaaaaca
   106621 agaaaacgaa ggatacaaaa taagtggtac aatagttact atagatttgg aggaattgta
   106681 tgagcaagga attacacatt aacacaattt tagcacaagc agggatcaag tctgatgagg
   106741 caacaggtgc tttggtgaca ccacttcatt tttcaacgac ctatcagcat ccagagtttg
   106801 gtcgatctac tgggtttgac tatacgcgca ctaaaaatcc aactcgtagt aaggctgagg
   106861 aagtcttggc ggctattgag tcagcagact atgccctagc gactagctca gggatgtcag
   106921 ctattgtact ggcctttagc gtctttccag taggaagtaa ggtcttggca gtgcgtgatc
   106981 tttacggtgg ttcttttcgc tggtttaacc aagtggagca ggaaggtcat ttccatttta
   107041 cctatgccaa tacagaagaa gagttgattg ccgagttaga aaaggatgtg gatgttctct
   107101 atatcgaaac cccaaccaat cccttgatgt tggaatttga tatcgaaaaa ctagcaaaat
   107161 tggctcatgc taagggttcc aaagtggtgg tggacaatac cttctatagc cctatttacc
   107221 aacgtccgat tgaagatgga gcagatatcg ttctccattc agcaaccaag tatctagcag
   107281 gccacaatga tgtcttggct ggagtggttg tgaccaatag tttagaacta tacgagaagc
   107341 ttttttacaa tcttaataca acaggggcag tcttgtctcc atttgacagc taccagttgc
   107401 ttcgtggtct caagaccttg tctcttcgta tggagcgctc aacagctaac gcccaagaag
   107461 tggttgcctt tttgaaggat tctccagcag ttaaggaagt tctctacact ggtcgtggag
   107521 gcatgatttc ctttaaagta gccgatgaaa cacgcattcc tcatattttg aacagtctca
   107581 aggtcttctc ttttgcggaa agtttgggcg gagtggaaag tcttattact tatccaacga
   107641 ctcaaactca tgctgatatt ccagcagaag tacgccattc ttatggtttg acagatgacc
   107701 tcttgcgttt gtctattggg attgaggatg ctagagattt gattgcagat ttgcgccaag
   107761 ccttagaagg ataagacaaa gatgggaaaa tatgatttta caagcctgcc caaccgttta
   107821 gggcaccata cctataaatg gaaagaaaca gaaacggata gtgaagttct accagcttgg
   107881 atagcggata tggactttgt ggtcttgcct gaaatccgcc aagccgtgca aacttacgca
   107941 gaccaactgg tttatggtta tacctatgcc agtgaagact taattaagga agttcaaaag
   108001 tgggaagcta cacaatacgg ttacaacttt gacaaagagg ctcttgtctt tatcgagggt
   108061 gtggtaccag ccatctcaac agctattcaa acctttacaa aagaaggcga ggcggtttta
   108121 attaacacgc ctgtctaccc accctttgct cgcagtgtca agttgaataa tcgtagattg
   108181 attactaatt ccttagtgga aaaggatagt ctgtttgaga ttgactttga ccaacttgaa
   108241 aaggatttgg tggaagagga ggttaaactc tatgttcttt gcaaccctca caatcctggt
   108301 ggacgtgttt gggaaaaaga agtgttggag aagattggcc aactctgcca aaaacacggt
   108361 gttttgttag tttcggatga gattcaccaa gatttgaccc tctttggtca caaacaccag
   108421 tctttcaata ccatcaatcc tgccttcaaa aattttgcta tcgtcttgag cagtgccact
   108481 aaaacattta atattgctgg aacaaaaaat tcctatgcag tcattgaaaa tcctaagttg
   108541 agactggctt tccagaaacg cctgttggcc aataatcagc atgaaatttc aggcttgggt
   108601 tatttggcga cagaagctgc ctatcgatac ggtaaagatt ggctagagga actcaagcaa
   108661 gtctttgaag accacatcaa ttatgtggtg gatctatttg gaaaagagac taaaatcaag
   108721 gtcatgaaac cgcaaggtac ctacttgatt tggcttgact tttcagccta tgacctgact
   108781 gatgaaacat tgcaagagtt gttgagaaat gaagccaagg ttatcctcaa ccgtggtttg
   108841 gattttggag aggaaggaag tctccatgcc cgcatcaata tagctatgcc caaatctctg
   108901 ttgcaagaag tctgtcagcg gattgtggct acttttgcca aatgttaaaa atccagcctt
   108961 ctaggagaaa agtcttccta gaaggctatt ttcataggcg aaaatatggt ataataaaaa
   109021 gataaggtaa aggtgaaaat atggctaaat tgattccggg gaaaattcgt atcgaaggtg
   109081 ttgcccttta tgaaactggt aaggttgata tcatcaagga aaagaacaat cggctctacg
   109141 ctcgcgttgc agaagaagaa ctgcgctata gtttagagga tgatttggtt ttttgtgcct
   109201 gtgatttttt tcaaaagagg ggctactgtg tgcatttggc agcgctagag cattttctga
   109261 aaaatgatga gcgtggtcag gaaatcttgt ggagtctgga agaaggtcat gaagaaaaag
   109321 aggccgttga aaccaaggtg accttgggtg gcaagttttt ggatcgaatt ttatctccaa
   109381 aatcagaatg cgcctatgag ttatcagctg tgggtcaggt ggaagcagga accaatcata
   109441 tcttgtggac tctgagaatc ggccaaatca atagccaaaa atactatgtc attcgggata
   109501 ttccactttt tttaaggatt gtcgagcaga gaaagtcgta tatgattggc aagatttatg
   109561 aggagtctct ttcttgggaa gcctttgatg atgccagcca agaacttttg actttcttgc
   109621 acggactaac ggaggaaggg ctggttccag acctcttttt ccaaaaccaa ggtcggcacc
   109681 tctttttccc tctgaccttt tttgagcagg gtgtgaattt actgatggcc ttgccccatt
   109741 ttcaatttga ccatcaagtg gatagctacc aaaccctgct ttttcaggat atgcatgctg
   109801 atgccaatct atttgccttt acagtaacag aatattcgga ctattttgaa atggaaatca
   109861 gtgagagtcc tagggtcaat gtcttttatc aaggagcagt acttttccat aaaggtcagg
   109921 tttatttttt aacagaccag cagatgcgtc ttctcaagga aattaaagcg ttacctgtgg
   109981 atcgacaagg aaagaaatac ctgcaatttg atagcagtga tcgagacaaa ttggcctcct
   110041 gtctgactct ttttatccag atgggcaccg tttcagctcc agaacgtcta caaatcaaaa
   110101 cttttgcgcc ctctttttac tttgacaggg aagaagataa tcggattcgt ttagaaattc
   110161 agtttgatta tggaaataga caggtatcta gccgacaaga gctagaagaa ttaccatttt
   110221 caagtgacgc ggacttagaa gaaagaattt tccaagtctg tttggctgcg ggctttgaag
   110281 ccgattttca atcttggcgt caggccttaa aagcggagtc tgtctatcat ttttttcatg
   110341 atatcattcc agtctttgaa aaactcgggc atgttgacct atcagataaa ctagaagagc
   110401 tttatagcct agcgagtcct caggtgcaga ttgcctccaa gggaggtctc ttggaaatcc
   110461 agtttgattt tcaagatatt gcccaggagg aaatcgacca agccatacag gccttggttg
   110521 ccaatcagga tttttatatt gattcgtcta atcaagttta ctttttcgat gaagaaacca
   110581 agaaaattcg ccaaaatcta caggaactgg ggcaatttga attaaaagat gggaccttgc
   110641 aggctcggaa atccttagcc tacagtttag ctcatctctt tgaagggcga gaccgtgttt
   110701 ctttttcaca agaattccag aatctggccc aggatttgac acatccagag gactttcctt
   110761 tgcaagcaac tcaggtcaag actgacttgc gagattatca ggaaaaggga attggctggt
   110821 tgcagatgct ccatcattat ggttttggtg ggattttggc tgatgatatg ggacttggga
   110881 aaacccttca gaccattgct tttttgacta gtcaagtgac aaaagaaagt cgagtcttga
   110941 ttctcgctcc gtcgggtttg atttacaact gggcggatga gtttcagaaa tttgctccac
   111001 agttggatgt ggctgttgtt catggtttga aagcaagtcg tgaggagatt cttgccgata
   111061 accatcaaat ctatgtgact agctatgcca ccttccgtca ggacagtgag ctttatcagg
   111121 ggatggcctt tgacttcctt ttcttagatg aagctcaggt catgaaaaat gcccagacca
   111181 agattgccca gaccttgaga caatttgtgg tgccgtcggt ctttgccttg tcaggaactc
   111241 cgattgaaaa ccatctgggt gagttgtggt ctattttcca aatcgtcatg cccggacttt
   111301 tgccaagcaa gaaagaattt atgaaattgc cagcagagcg agtggctcag tttatcaagc
   111361 ctttcgtgat gcgacgcaag aaagaagaag ttctgactga attgccagac ttgattgagg
   111421 tgatttataa aaatgaactg gaagaccagc aaaaggctat ttaccttgcc cagttacaac
   111481 agatgcgaga ccatctggct caagtgtcag aacaggaatt tcagcgaagt cgtgtggaaa
   111541 ttttatctgg tttgatgcgc ttgcgtcaaa tatgtgacac tcctgccctg tttatggaag
   111601 attatcaggg agccagcggc aaactagata gtctccgaga cctactggta caggtggcag
   111661 acggcggaca ccgtgtcttg attttctctc agttcaaggg aatgttggaa aaaattgaac
   111721 aagaactgcc agacttgggc ctgacttcct ttaaaattac gggttcaacc ccagccaagg
   111781 aaagacaaga catgaccaag gcctttaacc aaggagaaag agatgccttt ctgatttccc
   111841 tcaaggctgg tggggtcggt ctgaacctga caggtgcaga tacagtgatc ttggttgacc
   111901 tttggtggaa tcctgcggtg gaagcgcaag ccattggccg tgcccatcgg atgggtcagg
   111961 aagaaacggt tgaggtctat cgcttggtga ccaagggaac cattgaagaa aaaattcagg
   112021 aactccaaga acaaaagaaa catctggtgt cacaagtatt ggacggcaca gagtcacgtg
   112081 gtagtctgac cctagcagaa attagagaaa ttttgggaat ttctgaagcc agcacttgaa
   112141 aaatcaagac aatacgctat aatgaagtgt tgaaaggaaa tagaaaatga aacaagaacg
   112201 atttccattg gtgtcagatg acgaggtcat gttgactgaa atgccagtca tgaatctcta
   112261 tgatgagtct gatctgatca gtaatatcaa gggtgagtat cgagataaaa attatttaga
   112321 atgggctcct attgctgaag aaaaaccagt aaaaccgatt gaaaagcaag tagggaaacc
   112381 taaaaaagct cctttagggg ttaaaaaaga aggaaagagc tatgcggagg tggcgcgtga
   112441 agaagcgcgt gcagacttga aaaagaaacg ctctgctaac tatctaactc aggatttcag
   112501 ccttgcgaga cgtcattctc agcccagtct agttagacag ggcaatcaac cgacaactcc
   112561 tttccaaaag gaaaatcctg gtgaatttgt caaatatagc caaaaattga cccagtctca
   112621 ttatatcttg gcggaagaag ttcattctat ccctaccaag aatgaagaag tgtcagcacc
   112681 tgctccaaag aaaaacaatt atgattttct aaagaagagc caaatctaca ataaaaaaag
   112741 taaacaaaca gaacaagaac gtcgggttgc ccaagagttg aatctgacca gaatgacaga
   112801 ataggggaga aaacatgtca aagacatatc attttatcgg aattaaggga tcagggatga
   112861 gtgccttggc cttgatgttg taccagatgg ggcacaaggt tcagggatca gatgttgaaa
   112921 agtactactt tacccaacgc ggtcttgagc aggcaggaat taccattctt ccttttgatg
   112981 aaaagaatct agacggtgat atggaaatta tcgctggaaa tgcctttcgt ccagataaca
   113041 acgtcgaaat tgcctatgcg gaccaaaatg gtatcagcta caaacgttac catgagtttc
   113101 taggtagctt tatgcgtgac tttgttagca tgggagtagc aggagcacat ggaaaaactt
   113161 caacgacagg tatgttgtct catgtcttgt ctcacattac agataccagc ttcttgattg
   113221 gagatgggac aggtcgtggt tcggccaatg ccaaatattt tgtctttgaa tctgacgaat
   113281 atgagcgtca cttcatgcct taccacccag aatactctat tatcaccaac attgactttg
   113341 accatccaga ttatttcaca agtctcgagg atgttttcaa tgcctttaac gactatgcca
   113401 aacaaatcac caagggtctt tttgtctatg gtgaagatgc tgaattgcgt aagattacgt
   113461 ctgatgcacc aatttattat tatggttttg aagctgaagg caatgacttt gtagctagtg
   113521 atcttcttcg ttcaacaact ggttcaacct tcaccgttca tttccgtgga caaaacttgg
   113581 ggcaattcca cattccaacc tttggtcgtc acaatatcat gaatgcgaca gccgttattg
   113641 gtcttcttta cacagcagga tttgatttga acttggtgcg tgagcacttg aaaacatttt
   113701 ccggtgttaa acgtcgtttc actgagaaaa ttgtcaatga tacagtgatt atcgatgact
   113761 ttgcccatca tccaacagaa attattgcga ccttggatgc ggctcgtcag aaatacccaa
   113821 gcaaggaaat tgtagcagtc tttcaaccgc atacctttac aagaaccatt gccttgttgg
   113881 acgactttgc ccatgcttta aaccaagcag atgctgttta tctagcgcaa atttatggct
   113941 cggctcgtga agtagatcat ggtgacgtta aggtagaaga cctagccaac aaaatcaaca
   114001 aaaaacacca agtgattact gttgaaaatg tttctccact cctagaccat gacaatgctg
   114061 tttacgtctt tatgggagca ggagacatcc aaacctatga atactcattt gagcgtctct
   114121 tgtctaactt gacaagcaat gttcaatagg atgttcccat ggaaattcca attaagatca
   114181 ttcaggcaag caagtctgat ttgcctgaga taggggcact tcagacctcg tcttttccag
   114241 ctgaaaagca gcaactttcc catattttag aagaaagtat ccgtaagtgt gcggatacct
   114301 ttcttctagc tagggatgaa aatcaacttt taggctatat tttatcaagt ccccagtcag
   114361 acaatccgca atatctaaaa gtacattctt tagtcatcga gtctgaccat cagagacagg
   114421 gcttgggaac acttcttctt gcagccttga aagaggtggc agttgagctg gattacaaag
   114481 ggattcgttt ggagagtcct gatgagctgc tttcctattt tgaaatgaac ggttttgttg
   114541 atgaagaagc aactttgctc tatgcaacta gccagggcta tagtatgatt tggtttaatc
   114601 ccttttatct ggaggaacaa tgaaaatcag acaagcaaga ttagaagatt tggatcggat
   114661 tgttgaactt gaatttgaaa atttttcggt cgaagaagcc attcctcctt ctgtctttga
   114721 agcacatttg agagaaattc agacctcttt tctggttgct gaaaaagaag gaagaatcat
   114781 gggttatatc gaaggaccag ttggcctgca ccgccatctg caagaccagt cttttacaga
   114841 agaaataaaa gactatagtc atgagcctgg tggttatatc tctgtgacct gtctgtctat
   114901 tgccaaggag gcacagggac tcggactggg tcagaaattg ctgacagcct tgaaagaagt
   114961 tgctcttgaa gatgagagag atggcattaa tctaacctgt catgactatc tcatcgccta
   115021 ctatgaaaaa catggatttg tcaacgaagg ccagtcccag tcaacctttg caggggaaac
   115081 atggtatgat atggtctggg aaatgaaaaa ataagttagg aaaagtatca tagattgctt
   115141 tttttggact tttaagatat aatattatgg attatcaaca aggaggaaaa acttttgagt
   115201 gaaaagtcaa gagaagaaga gaaattaagc tttaaagagc agattctgag agatttagaa
   115261 aaagtaaaag gctatgatga agttctgaaa gaagatgagg cagtagttcg cactcctgca
   115321 aatgaacctt caactgaaga actcatggct gattccttgt caacggtaga ggagattatg
   115381 agaaaagctc ctaccgtgcc tactcaccca agtcaaggtg taccagcttc tccagcagat
   115441 gagattcaaa gagaaactcc tggtgttcca agtcatccaa gtcaagatgt accttcttct
   115501 ccagcggaag aaagtggatc aagaccaggt ccaggtcctg ttagacctaa gaaacttgaa
   115561 agagaataca atgaaacccc aacaagggta gctgtttcct atacgacggc agagaaaaaa
   115621 gcagaacaag caggtccaga aacacctacg cctgctacag aaacagtgga tatcatcaga
   115681 gatacatcac gtcgtagccg tagagaagga gcaaaacccg ttaagcctaa gaaagagaag
   115741 aagtcacatg tgaaagcttt tgtgatttca ttccttgtat tccttgcctt gctctcagca
   115801 ggtggttact ttggttacca gtacgtgcta gattccttat tacctatcga tgctaattct
   115861 aagaaatatg tgacggttgg aattccagaa ggttcaaacg ttcaagaaat cggtacgacg
   115921 cttgaaaaag ctggtttggt aaagcatggt ctgattttta gtttttatgc caagtataaa
   115981 aattataccg acttgaaagc aggttactac aatttgcaaa agagtatgag tacagaagac
   116041 ttactcaaag agttgcaaaa aggtggaaca gatgaaccgc aagaacctgt acttgcgact
   116101 ttgacaattc cagaaggtta taccttggat cagattgctc aagctgtggg tcaattgcaa
   116161 ggtgacttca aagagtcttt gacagcggag actttcttgg ctaaagttca agatgagacg
   116221 tttatcagtc aagcagtagc gaaatatcct actttactgg aaagtttgcc tgtaaaagac
   116281 agcggtgcgc gttatcgttt ggaaggatac cttttcccag ctacatactc tatcaaggaa
   116341 agcacaacta ttgagagctt gattgatgag atgttagctg ctatggataa gaacctatct
   116401 ccttactata gtactatcaa atctaaaaac ttgactgtca atgagttgtt gaccattgct
   116461 tccttggtcg aaaaagaagg tgccaagaca gaagatcgta agctcattgc aggtgtattc
   116521 tacaatcgtt tgaatcgtga tatgccactt caaagtaata ttgcaatctt gtatgcccaa
   116581 ggaaaactgg ggcaaaatat cagtctagct gaggatgttg cgattgatac caacattgat
   116641 tcaccttata atgtttataa aaatgtaggt ctcatgcctg gtccagtcga tagtccaagt
   116701 ctggatgcga ttgagtcaag catcaatcaa actaagagcg ataacctcta ctttgtagca
   116761 gatgtcacag aaggcaaggt ctactatgct aacaatcaag aagaccacga ccgcaatgtc
   116821 gctgaacatg tcaacagcaa attaaactaa acaaactaaa attatgttat acttcacata
   116881 attttcttta gaaaatatta tcagaagaaa gttgagaaaa atggcagaaa aaacatatcc
   116941 tatgaccctt gaggaaaagg aaaaacttga aaaagaatta gaagaattga aattggttcg
   117001 tcgaccagaa gtggtagaac gcattaagat tgcccgttca tacggtgacc tttcagaaaa
   117061 cagtgagtac gaagcagcta aggatgaaca agcctttgtc gaaggacaaa tctctagctt
   117121 agaaacaaaa atccgctatg ctgaaatcgt caatagcgac gcagttgccc aggacgaagt
   117181 agcgattggt aaaacagtca ccatccaaga aattggtgag gacgaagaag aagtttatat
   117241 tatcgtaggt tcagctggtg cggatgcctt tgcaggtaag gtttcaaatg aaagcccaat
   117301 tgggcaggcc ttgattggca agaaaacagg tgatacagca accattgaaa cgcctgttgg
   117361 tagctatgat gtaaaaatct tgaaggttga aaaaacagcc taaaaacaga aaaaggagtg
   117421 gggaggcgat gtgcttcact cactcctttt tccattttgc tattcttcga aaatctcttc
   117481 aaaccacgtc agcgtcgcct tgccgtatgt atggttactg acttcgtcag tttcatctac
   117541 aacctcaaaa ccatgttttg agccgacttc gtcagtttca tctacaacct caaaaccatg
   117601 ctttgagctg acttcgtcag tttcatctac aacctcaaaa ccatgctttg agctgacttc
   117661 gtcagtttca tctacaacct caaaaccatg ctttgagctg acttcgtcag ttctatctac
   117721 aacctcaaaa cagtgttttg agcaacctgc ggctagtttc ctagtttgct ctttgatttt
   117781 tattgagtat tagatgtata gtgcattgaa gttaaaatag tacactttga tttctaagac
   117841 attgttagaa attagattaa attccttaat cgatttgcgc atgttttatt tcatttcact
   117901 atactttaga ttggaaaata aggaatcaca taaatcgcaa gaaataggga atctgattcg
   117961 tgtttataac cgttcaaaaa gagaagaggc tgaaagtgag ccacttaatc tttatgtcga
   118021 agatgaaaag ggcaatctcc tggcaggttt gatagcagag acttttggaa attggctaga
   118081 aatcgagtat ttgtttgtaa aagaggaact gcgagggcaa ggaatcggtt caaaactatt
   118141 gcaacaggca gaaagtgaag ctaagaatcg aaattgttgt tttgcctttg tcaatactta
   118201 ccagttccag gcaccggact tttatcagaa acatggctac aaggaagttt tttctttgca
   118261 agactatctc tacattaggc aaagatatta ttaccaaaag aatctgtaag gtgagtatgg
   118321 aggtataata caaagagagg ctcgcacata agtatggagg tggactgatg tatatgagta
   118381 actatcgata aataccagtt ataacctaga aattgtgtct ttctagacga tattgaggac
   118441 aatataatcg tagccccaaa attaggaatc aagacttatc aggttaagaa aagaagtgat
   118501 gttgttgata ttttaaacaa tttatttaaa ctaaaaactc tctatctatt aatccgagat
   118561 tcccctctcg gattttgtgt tgctttcatt aaatttacag gtaagacttg ctagcttcag
   118621 taaatataca caagtttttc agaatagagt gaaatccaaa agaaaatgga aataatgaaa
   118681 gctcttgtag gcataagaaa acacctctgt gttatacttg ttgttcaacc acaaacacaa
   118741 gaaaggcaca gagatgcaag acaattatac tacaaaagcc aaacatttga caatcgatag
   118801 ccgtcgctta atcgaaagat ggaaaaaaga agggaaatca aatagagaaa ttgcctctct
   118861 gcttggaaaa gttcctcaaa ctatccacac tgaaatcaag cgtgggacag tccgacaatg
   118921 tcttggaaaa gggcgcttca aagaggttta ttctgccgac tatgctcaac agtcttatga
   118981 aaataatcgc aagcgctcgg tcaagaaatc aagcttgacc aaggaactaa aggaaaagat
   119041 tctccactat cataaccaaa aattttcgcc tgaaatgatg gtgatggcta aaggggttaa
   119101 cgtgggaatt tcaaccattt actattggat tcatcatgga aaattggggt taagcaaaca
   119161 ggatctgctt taccctagaa aaggaaaagc gcttaagaaa caggctagca ccaactttaa
   119221 acctgctggt caatccatcg aacagcggtc tgaagctatc aatcttcgct tggagaatgg
   119281 gcattatgag attgatacgg ttctacttac gagagcgaaa aactactgct tgcttgtctt
   119341 gacggatcga aagagtagac atcagatcat ccgattgatt ccaaataaaa gtgctgaggt
   119401 ggtcaatcag gctctaaaac tcatcttaaa acaacacaag attctttcca tcacggcaga
   119461 taatggaacg gaattcaatc gcttgtttga tgtattttct gaggagcaca tctattatgc
   119521 gcacccctat gcctcttggg aaaggggaac taatgagaat cacaacaggc tcattcgtag
   119581 atggttacct aagggaacca agaaaatgac tcccaaagaa gtcgcattca tcgaaaagtg
   119641 gattaacaac tatcctaaaa aatgcttgga ctacaagtca cccagagaag acttctggat
   119701 ggctaacttg aacttgaaat ttagcaaaat ggaaataatt tttattaaac gcttccaata
   119761 gcggttaaaa gttgacaaat ttaaatttta ggactacact aagtaagtaa attttatttt
   119821 aaaggagaat ttgttatgaa tttaacattt ttaggcttat gtattgcctg tatgggcgta
   119881 tctgtcggtg aaggtttatt gatgaatgga ctgtttaaat cagtagcacg ccaaccagat
   119941 atgctttctg agtttcgtag tttgatgttt ttaggtgttg cctttattga aggaactttc
   120001 tttgtaactc ttgtcttctc atttattatc aaataaatac atggaacgag aagaaaaggg
   120061 aggattttag atggaagaaa gtattaatcc aatcatctct attggtcctg ttatcttcaa
   120121 tctgactatg ttagccatga ctttgttgat tgtgggagtt atttttgtct ttatttattg
   120181 ggcaagccgc aatatgacct tgaaacccaa aggaaagcaa aatgtacttg agtatgtcta
   120241 tgactttgtt attggattta cagaacctaa cattggttcg cgctacatga aagattactc
   120301 actctttttc ctttgtttat tccttttcat ggtgattgcc aataaccttg gcttaatgac
   120361 aaagcttcaa acgatcgatg ggactaactg gtggagttcg ccaaccgcta atttacagta
   120421 tgacttaacc ttatcttttc ttgtcatttt gttgacacat atagaaagcg ttcgtcgtcg
   120481 tggatttaaa aaaagtataa aatcttttat gagtcctgtt tttgtcatac cgatgaatat
   120541 cttggaagaa tttacaaact tcttatcttt ggctttgcgg atttttggga atatctttgc
   120601 aggagaggtc atgacgagtt tgttacttct tctttcccac caagctattt attggtatcc
   120661 agtagccttt ggagctaatt tggcttggac tgcattttct gtctttattt cctgcatcca
   120721 agcttatgtt tttactcttt tgacatctgt gtatttaggg aataagatta atattgaaga
   120781 ggaatagaaa ggagtaactg atgcacgtaa cagtaggtga attaattggt aattttattt
   120841 taatcactgg ctcttttatt cttttgctag tcttgattaa aaaatttgca tggtctaata
   120901 ttacaggcat tttcgaagaa agagctgaaa aaattgcttc agatattgac agagctgaag
   120961 aagcccgtca aaaagcagaa gtattggctc aaaaacgcga agatgaattg gctggtagcc
   121021 gtaaagaagc taagacaatc attgaaaatg caaaggaaac agctgagcaa agtaaggcta
   121081 atatcttagc agatgctaaa ctagaagcag gacacttaaa agaaaaagcc aatcaagaaa
   121141 ttgctcaaaa taaagtagaa gctttacaga gtgttaaggg tgaggtcgca gatttgacca
   121201 tcagcttagc tggtaaaatc atctcacaaa accttgacag tcatgcccat aaagcactca
   121261 ttgatcagta tatcgatcag ctaggagaag cttaatggac aagaaaacag taaaggtaat
   121321 tgaaaaatac agcatgcctt ttgtccaatt ggtacttgaa aaaggagaag aagaccgtat
   121381 cttttcagac ttgactcaaa tcaagcaagt tgttgaaaaa acaggtctgc cttctttttt
   121441 aaaacaagtg gcagtagacg agtcggataa ggaaaaaaca attgcttttt tccaagattc
   121501 tgtgtcgcct ttattacaaa actttatcca ggttctggcc tacaatcaca gagcaaatct
   121561 tttttatgat gtgcttgtag attgcttgaa ccgacttgaa aaagaaacaa atcgatttga
   121621 agtgacgatt acgtctgctc atcctctaac tgatgaacag aagactcgtt tgctcccttt
   121681 gattgagaaa aaaatgtctc tgaaagtaag gagtgtaaaa gaacaaatcg atgaaagtct
   121741 cattggtggt tttgtcattt ttgccaatca caagacaatt gatgtgagta ttaaacaaca
   121801 acttaaagtt gttaaagaaa atttgaaata gaaagtggtg ttcttttggc aattaacgca
   121861 caagaaatca gcgctttaat taagcaacaa attgaaaatt tcaaacccaa ttttgatgtg
   121921 actgaaacag gtgttgtaac ctatatcggg gacggtatcg cgcgtgctca tggccttgaa
   121981 aatgtcatga gtggagagtt gttgaatttt gaaaacggct cttatggtat ggctcaaaac
   122041 ttggagtcaa cagacgttgg tattatcatc ctaggtgact ttacagatat ccgtgaaggc
   122101 gatacaatcc gccgtacagg gaaaatcatg gaagtccctg taggtgaaag tctgattggt
   122161 cgtgttgtgg atccgcttgg tcgtccagtt gacggtcttg gagaaatcca cactgataaa
   122221 actcgtccag tagaagcacc agctcctggt gttatgcaac gtaagtctgt ttcagaacca
   122281 ttgcaaactg gtttgaaagc tattgacgcc cttgtaccga ttggtcgtgg tcaacgtgag
   122341 ttgattatcg gtgaccgtca gacagggaaa acaaccattg cgattgatac aatcttgaac
   122401 caaaaagatc aagatatgat ctgtatctac gtcgcgattg gacaaaaaga atcaacagtt
   122461 cgtacgcaag tagaaacact tcgtcagtac ggtgccttgg actacacaat cgttgtgaca
   122521 gcctctgctt cacaaccatc tccattgctc ttcctagctc cttatactgg ggttgctatg
   122581 gcggaagaat ttatgtatca aggtaagcat gttttgattg tatacgatga tctatcaaaa
   122641 caagcggtag cttatcgtga actatcgctc ttgcttcgtc gtcctccagg tcgtgaagcc
   122701 ttcccagggg atgttttcta tctccacagc cgtttgcttg agcgctcagc taaagtttct
   122761 gatgaacttg gtggtggatc aattacagcc ctaccattta tcgagacaca agcaggagat
   122821 atctcagcct atatcgcaac caacgtgatt tctatcactg atggacaaat cttccttggc
   122881 gatggcctct tcaatgcagg tattcgtcca gccatcgatg cgggttcatc tgtatctcgt
   122941 gtaggtggtt ctgcacaaat caaagccatg aagaaggttg ctggtacact tcgtatcgac
   123001 cttgcttcat accgtgagtt ggaagccttt actaagtttg gttctgactt ggacgcagca
   123061 acacaggcta agttgaaccg tggacgtcgt accgttgagg tcttgaaaca acctgttcac
   123121 aaaccattac ctgttgagaa acaagtaacc attctttatg ctttgacaca tggtttcttg
   123181 gatactgttc cagtagatga tattgttcgt ttcgaggaag agttccatgc cttctttgat
   123241 gctcaacatc cagagatttt ggaaaccatt cgtgatacaa aagacttgcc agaagaagca
   123301 gtcttggatg ctgcgattac agagtttctc aatcaatcta gcttccaata agaatagagg
   123361 tgtcagatgg cagtgtctct aaatgatatt aaaacaaaaa tcgcctcaac aaaaaatacg
   123421 agtcaaatca ctaatgccat gcaaatggta tcggctgcta agttaggtcg ctctgaagaa
   123481 gctgctcgca acttccaagt ttacgctcag aaagtgcgta aacttttgac agatatcctt
   123541 catggtaatg gagctggtgc ttcaactaat ccgatgttga ttagccgttc tgtgaagaag
   123601 acaggctata tcgttatcac ttcagaccgc ggtttggttg gaggttataa ttcctctatt
   123661 ttgaaagctg ttatggagtt gaaagaagaa taccacccag acggtaaagg ttttgaaatg
   123721 atctgtatcg gtgggatggg agctgatttc tttaaggctc gcggtattca accactttat
   123781 gaattacgtg gcttggcaga ccaacctagc tttgatcaag ttcgtaagat tatttcaaaa
   123841 actgttgaaa tgtaccaaaa tgaactcttt gatgagcttt atgtttgcta caaccaccat
   123901 gtcaatacgc taaccagtca aatgcgtgtg gaacaaatgc ttccgattgt tgacttggat
   123961 ccaaatgaag cggatgaaga gtacagcttg acttttgaat tggaaaccag ccgagaagaa
   124021 attctggagc agttgttgcc tcagtttgca gaaagtatga tttacggtgc cattatcgat
   124081 gccaagacag ctgagaatgc tgcgggtatg acagccatgc aaacagcgac agataatgct
   124141 aagaaagtca tcaatgattt gacaattcag tataaccgtg ccagacaggc ggcgattaca
   124201 caagaaatta cagaaatcgt agcaggtgct agtgccttag aataggctct agtccagctc
   124261 gtatgaaaat gaacttagga cctagttgag ctaggaaccg acagtatctt atatagaata
   124321 ggagaaggag atgagttcag gtaaaattgc tcaggttatc ggtcccgttg tagacgtttt
   124381 gtttgcagca ggggaaaaac ttcctgagat taacaatgca cttgtcgtct acaaaaatga
   124441 cgaaagaaaa acaaaaatcg tccttgaagt agccttggag ttaggagatg gtatggttcg
   124501 tactatcgcc atggaatcaa cagatgggtt gactcgtgga atggaagtat tggacacagg
   124561 tcgtccaatc tctgtaccag taggtaaaga aactttggga cgtgtcttca acgttttggg
   124621 agataccatt gatttggaag ctccttttac agaagacgca gagcgtcagc caattcataa
   124681 aaaagctcca acttttgatg agttgtctac ctcttctgaa atccttgaaa cagggatcaa
   124741 ggttattgac cttcttgccc cttaccttaa aggtggtaaa gttggacttt tcggtggtgc
   124801 cggagttggt aaaaccgtct taatccaaga attgattcac aacattgccc aagagcacgg
   124861 tggtatttca gtatttactg gtgttgggga acgtactcgt gaggggaatg acctttactg
   124921 ggaaatgaaa gaatcaggcg ttatcgagaa aacagccatg gtctttggtc agatgaatga
   124981 gccaccagga gcacgtatgc gtgttgccct tactggtttg acaatcgctg aatacttccg
   125041 tgatgtggaa ggccaagacg tgcttctctt tatcgataat atcttccgtt tcactcaggc
   125101 tggttcagaa gtatctgccc ttttgggtcg tatgccatca gccgttggtt accaaccaac
   125161 acttgctacg gaaatgggtc aattgcaaga acgtatcaca tcaaccaaga agggttctgt
   125221 aacctctatc caggctatct atgtgccagc ggatgactat actgacccag cgccagcaac
   125281 agccttcgct cacttggatt caacaacaaa cttggaacgt aaattggtac aattgggtat
   125341 ctacccagcc gttgacccac ttgcttcaag ctcacgtgcc ttggcacctg aaatcgttgg
   125401 agaagagcac tatgcagttg ctgctgaagt aaaacgtgtc cttcaacgtt accatgaatt
   125461 gcaagatatc attgctatcc ttggtatgga tgagctttct gatgaagaaa agaccttggt
   125521 tgctcgcgcc cgtcgtatcc agttcttctt gtcacaaaac ttcaacgttg cggaacaatt
   125581 tactggtcag ccaggttctt atgttccagt tgctgaaact gtacgtggct ttaaggaaat
   125641 ccttgatggt aaatacgacc acttgccaga agatgccttc cgtggtgtag gttctatcga
   125701 agatgtgatt gcaaaagctg aaaaaatggg attttaagag gtgacctatg gctcagttaa
   125761 ctgtccagat cgtgacacca gatggtctcg tctatgatca ccatgccagc tatgtatcgg
   125821 ttcgaactct ggatggtgag atggggatct tgccacgaca tgaaaatatg attgcggttt
   125881 tagcagttga tgaagtaaag gtaaaacgta tcgatgataa agatcacgtg aactggattg
   125941 cagtaaacgg tggcgttatt gaaattgcca atgatatgat cacaatcgtc gctgactctg
   126001 cagaacgtgc tcgtgatatc gatattagcc gtgcagaacg tgccaaactt cgtgcagaac
   126061 gtgcaattga agaagcacaa gacaaacatt tgattgacca agaacgtcgt gctaagattg
   126121 ctttgcaacg tgccattaac cgtattaatg tcggaaatag actataagaa aaaatgaact
   126181 tgaaaatacc aagttcattt tttaggctct ttgtcaactg tagtgggttg aaaaaaagct
   126241 aagctcgaga aaggacaaat tttgtccttt cttttttgat attcagagcg ataaaaatcc
   126301 gttttttgaa gttttcaaag ttccgaaaac caaaggcatt gcgcttgata agtttgatga
   126361 gattattggt cgcttccaat ttggcgtttg aatagggtag ttgaagggcg ttgacgattt
   126421 tctctttgtc ctttagaaag gttttaaaga cagtctgaaa aagaggatga acctgcttaa
   126481 gattgtcctc aataagtccg aaaaatttct ctggttcctt attctgaaag tgaaacagca
   126541 agagttgata gagctgatag tgatgtttca agtcttgtga atagctcaaa agcttgtcta
   126601 aaatctcttt attggttaaa tgcatacgaa aagtagggcg ataaaaatgt ttatcgctga
   126661 gtttacg
//
LOCUS       DAVKLX010000002        68406 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKLX010000002 DAVKLX010000000
VERSION     DAVKLX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 68406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68406
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.002"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            444..1919
                     /locus_tag="VW325_000128"
     CDS             444..1919
                     /locus_tag="VW325_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9733608.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(1885..2600)
                     /locus_tag="VW325_000129"
                     /pseudo
     CDS             complement(1885..2600)
                     /locus_tag="VW325_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2856..3716
                     /gene="speE"
                     /locus_tag="VW325_000130"
     CDS             2856..3716
                     /gene="speE"
                     /locus_tag="VW325_000130"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9733609.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            3713..4972
                     /locus_tag="VW325_000131"
     CDS             3713..4972
                     /locus_tag="VW325_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9733610.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            4972..6099
                     /gene="nspC"
                     /locus_tag="VW325_000132"
     CDS             4972..6099
                     /gene="nspC"
                     /locus_tag="VW325_000132"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9733611.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6096..7181
                     /gene="aguA"
                     /locus_tag="VW325_000133"
     CDS             6096..7181
                     /gene="aguA"
                     /locus_tag="VW325_000133"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9733612.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7191..8066
                     /gene="aguB"
                     /locus_tag="VW325_000134"
     CDS             7191..8066
                     /gene="aguB"
                     /locus_tag="VW325_000134"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9733613.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGGVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8247..9056)
                     /locus_tag="VW325_000135"
     CDS             complement(8247..9056)
                     /locus_tag="VW325_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9733614.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            9328..9549
                     /locus_tag="VW325_000136"
     CDS             9328..9549
                     /locus_tag="VW325_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU9733615.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCSRGTYDLGYIGGGNHLCKGSAARF"
     gene            9603..10274
                     /locus_tag="VW325_000137"
     CDS             9603..10274
                     /locus_tag="VW325_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9733616.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(10326..10523)
                     /locus_tag="VW325_000138"
     CDS             complement(10326..10523)
                     /locus_tag="VW325_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9733617.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            10516..11424
                     /locus_tag="VW325_000139"
     CDS             10516..11424
                     /locus_tag="VW325_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9733618.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11421..11882
                     /locus_tag="VW325_000140"
     CDS             11421..11882
                     /locus_tag="VW325_000140"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9733619.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            11872..12759
                     /locus_tag="VW325_000141"
     CDS             11872..12759
                     /locus_tag="VW325_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9733620.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            12762..14645
                     /gene="cbpE"
                     /locus_tag="VW325_000142"
                     /gene_synonym="pce"
     CDS             12762..14645
                     /gene="cbpE"
                     /locus_tag="VW325_000142"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9733621.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            14725..15855
                     /locus_tag="VW325_000143"
     CDS             14725..15855
                     /locus_tag="VW325_000143"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9733622.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            15865..17127
                     /locus_tag="VW325_000144"
     CDS             15865..17127
                     /locus_tag="VW325_000144"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9733623.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            17131..17928
                     /gene="proC"
                     /locus_tag="VW325_000145"
     CDS             17131..17928
                     /gene="proC"
                     /locus_tag="VW325_000145"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9733624.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            18148..18786
                     /locus_tag="VW325_000146"
     CDS             18148..18786
                     /locus_tag="VW325_000146"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9733625.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILKEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            18783..19673
                     /locus_tag="VW325_000147"
     CDS             18783..19673
                     /locus_tag="VW325_000147"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9733626.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLQT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            19713..20030
                     /gene="yabA"
                     /locus_tag="VW325_000148"
     CDS             19713..20030
                     /gene="yabA"
                     /locus_tag="VW325_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9733627.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            20033..20902
                     /gene="rsmI"
                     /locus_tag="VW325_000149"
     CDS             20033..20902
                     /gene="rsmI"
                     /locus_tag="VW325_000149"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9733628.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETSLKGECLLIVEGASQDVEEKGEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            21129..21647
                     /locus_tag="VW325_000150"
     CDS             21129..21647
                     /locus_tag="VW325_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9733629.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENIHSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            21756..21893
                     /locus_tag="VW325_000151"
     CDS             21756..21893
                     /locus_tag="VW325_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9733630.1"
                     /translation="MDFEYFYNREAKRFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     K"
     gene            21900..22334
                     /locus_tag="VW325_000152"
     CDS             21900..22334
                     /locus_tag="VW325_000152"
                     /inference="COORDINATES: protein motif:HMM:NF018653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9733631.1"
                     /translation="MSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDIK
                     KGIGLIERVRLGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQ
                     EVKNLDSNYIENNKSKYSKREYSFGENGLGTFQNVFLAAEDI"
     gene            complement(22392..23199)
                     /locus_tag="VW325_000153"
     CDS             complement(join(22392..22826,22828..23199))
                     /locus_tag="VW325_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9733632.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIIDATEVKINRPKKELANYSGKKKCYAMKAQAIVTSQGRIVSLDITVN
                     YCHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKFKPLTVEDKTYNH
                     ALSKERSKVENIFAKVETFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            23194..23448
                     /locus_tag="VW325_000154"
     CDS             23194..23448
                     /locus_tag="VW325_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9733633.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            23792..25126
                     /gene="trmFO"
                     /locus_tag="VW325_000155"
     CDS             23792..25126
                     /gene="trmFO"
                     /locus_tag="VW325_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9733634.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            25210..25947
                     /locus_tag="VW325_000156"
     CDS             25210..25947
                     /locus_tag="VW325_000156"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9733635.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            25956..26513
                     /gene="frr"
                     /locus_tag="VW325_000157"
     CDS             25956..26513
                     /gene="frr"
                     /locus_tag="VW325_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9733636.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            26573..27427
                     /gene="cvfB"
                     /locus_tag="VW325_000158"
     CDS             26573..27427
                     /gene="cvfB"
                     /locus_tag="VW325_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9733637.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPVLKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            27436..27651
                     /locus_tag="VW325_000159"
     CDS             27436..27651
                     /locus_tag="VW325_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9733638.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            27737..28705
                     /locus_tag="VW325_000160"
     CDS             27737..28705
                     /locus_tag="VW325_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9733639.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(28683..28862)
                     /locus_tag="VW325_000161"
     CDS             complement(28683..28862)
                     /locus_tag="VW325_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733640.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            28898..29398
                     /locus_tag="VW325_000162"
     CDS             28898..29398
                     /locus_tag="VW325_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9733641.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVIDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            29401..29661
                     /locus_tag="VW325_000163"
                     /pseudo
     CDS             29401..29661
                     /locus_tag="VW325_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(30028..30354)
                     /locus_tag="VW325_000164"
     CDS             complement(30028..30354)
                     /locus_tag="VW325_000164"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733642.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(30300..30713)
                     /locus_tag="VW325_000165"
     CDS             complement(30300..30713)
                     /locus_tag="VW325_000165"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU9733643.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(30714..31139)
                     /locus_tag="VW325_000166"
     CDS             complement(30714..31139)
                     /locus_tag="VW325_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU9733644.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            31316..31885
                     /locus_tag="VW325_000167"
     CDS             31316..31885
                     /locus_tag="VW325_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9733645.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTPETNRAVIELAFEKIGMNKLVALYDKDNSASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            31953..32603
                     /locus_tag="VW325_000168"
     CDS             31953..32603
                     /locus_tag="VW325_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEU9733646.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            32587..34827
                     /locus_tag="VW325_000169"
     CDS             32587..34827
                     /locus_tag="VW325_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9733647.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKKIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPLSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGWNSW
                     KIESVR"
     gene            34969..35193
                     /locus_tag="VW325_000170"
     CDS             34969..35193
                     /locus_tag="VW325_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733648.1"
                     /translation="MIIGLFQSSISAVTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSW
                     SRYYSYSEYPVGSGWNYARYEVINYYTGGY"
     gene            35225..35812
                     /locus_tag="VW325_000171"
     CDS             35225..35812
                     /locus_tag="VW325_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9733649.1"
                     /translation="MLRLTHVTLKTRQVILQDADFTFKKGRVYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            35816..36997
                     /locus_tag="VW325_000172"
     CDS             35816..36997
                     /locus_tag="VW325_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733650.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVVVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     misc_feature    37157..37288
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            37304..37834
                     /gene="infC"
                     /locus_tag="VW325_000173"
     CDS             37304..37834
                     /gene="infC"
                     /locus_tag="VW325_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9733651.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            37867..38067
                     /gene="rpmI"
                     /locus_tag="VW325_000174"
     CDS             37867..38067
                     /gene="rpmI"
                     /locus_tag="VW325_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9733652.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            38119..38478
                     /gene="rplT"
                     /locus_tag="VW325_000175"
     CDS             38119..38478
                     /gene="rplT"
                     /locus_tag="VW325_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9733653.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            38536..38916
                     /locus_tag="VW325_000176"
     CDS             38536..38916
                     /locus_tag="VW325_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9733654.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            39166..39966
                     /locus_tag="VW325_000177"
     CDS             39166..39966
                     /locus_tag="VW325_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9733655.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            39977..40915
                     /locus_tag="VW325_000178"
     CDS             39977..40915
                     /locus_tag="VW325_000178"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9733656.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            40957..42990
                     /locus_tag="VW325_000179"
     CDS             40957..42990
                     /locus_tag="VW325_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU9733657.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(43051..44706)
                     /gene="pavA"
                     /locus_tag="VW325_000180"
     CDS             complement(43051..44706)
                     /gene="pavA"
                     /locus_tag="VW325_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9733658.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            44811..45308
                     /gene="ybeY"
                     /locus_tag="VW325_000181"
     CDS             44811..45308
                     /gene="ybeY"
                     /locus_tag="VW325_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9733659.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            45289..45684
                     /locus_tag="VW325_000182"
     CDS             45289..45684
                     /locus_tag="VW325_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9733660.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            45701..46600
                     /gene="era"
                     /locus_tag="VW325_000183"
     CDS             45701..46600
                     /gene="era"
                     /locus_tag="VW325_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9733661.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            46649..47473
                     /gene="mutM"
                     /locus_tag="VW325_000184"
     CDS             46649..47473
                     /gene="mutM"
                     /locus_tag="VW325_000184"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9733662.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            47473..48078
                     /locus_tag="VW325_000185"
     CDS             47473..48078
                     /locus_tag="VW325_000185"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9733663.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFEQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            48065..49264
                     /locus_tag="VW325_000186"
     CDS             48065..49264
                     /locus_tag="VW325_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEU9733664.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            49264..49413
                     /gene="rpmG"
                     /locus_tag="VW325_000187"
     CDS             49264..49413
                     /gene="rpmG"
                     /locus_tag="VW325_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9733665.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKEKKVTL
                     HLESK"
     gene            49453..49686
                     /gene="secG"
                     /locus_tag="VW325_000188"
     CDS             49453..49686
                     /gene="secG"
                     /locus_tag="VW325_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9733666.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            49788..52142
                     /gene="rnr"
                     /locus_tag="VW325_000189"
     CDS             49788..52142
                     /gene="rnr"
                     /locus_tag="VW325_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9733667.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIVEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGIGRGSNRRSDKKEDKRKS
                     GRSNDKRKYSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            52105..52572
                     /gene="smpB"
                     /locus_tag="VW325_000190"
     CDS             52105..52572
                     /gene="smpB"
                     /locus_tag="VW325_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9733668.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            52588..53448
                     /gene="tehB"
                     /locus_tag="VW325_000191"
     CDS             52588..53448
                     /gene="tehB"
                     /locus_tag="VW325_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9733669.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            53521..54474
                     /gene="coiA"
                     /locus_tag="VW325_000192"
     CDS             53521..54474
                     /gene="coiA"
                     /locus_tag="VW325_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9733670.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            54493..56295
                     /gene="pepF"
                     /locus_tag="VW325_000193"
     CDS             54493..56295
                     /gene="pepF"
                     /locus_tag="VW325_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9733671.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            56297..57010
                     /locus_tag="VW325_000194"
     CDS             56297..57010
                     /locus_tag="VW325_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9733672.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            57077..58018
                     /gene="prsA"
                     /locus_tag="VW325_000195"
     CDS             57077..58018
                     /gene="prsA"
                     /locus_tag="VW325_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9733673.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(58135..58476)
                     /locus_tag="VW325_000196"
                     /pseudo
     CDS             complement(58135..58476)
                     /locus_tag="VW325_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            58890..58985
                     /locus_tag="VW325_000197"
                     /pseudo
     CDS             58890..58985
                     /locus_tag="VW325_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(59239..59859)
                     /locus_tag="VW325_000198"
     CDS             complement(59239..59859)
                     /locus_tag="VW325_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9733674.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(59859..60341)
                     /locus_tag="VW325_000199"
     CDS             complement(59859..60341)
                     /locus_tag="VW325_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9733675.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(60341..61183)
                     /locus_tag="VW325_000200"
     CDS             complement(60341..61183)
                     /locus_tag="VW325_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9733676.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGALAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLTWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(61278..62078)
                     /locus_tag="VW325_000201"
     CDS             complement(61278..62078)
                     /locus_tag="VW325_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9733677.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            62222..63601
                     /gene="glmU"
                     /locus_tag="VW325_000202"
     CDS             62222..63601
                     /gene="glmU"
                     /locus_tag="VW325_000202"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9733678.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            63611..64156
                     /locus_tag="VW325_000203"
     CDS             63611..64156
                     /locus_tag="VW325_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9733679.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            64168..64482
                     /locus_tag="VW325_000204"
     CDS             64168..64482
                     /locus_tag="VW325_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9733680.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            64499..65191
                     /locus_tag="VW325_000205"
     CDS             64499..65191
                     /locus_tag="VW325_000205"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9733681.1"
                     /translation="MKIGIIAAMPEELAYLVQHLYNVQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHTLNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            65268..65759
                     /gene="rocS"
                     /locus_tag="VW325_000206"
     CDS             65268..65759
                     /gene="rocS"
                     /locus_tag="VW325_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9733682.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            65857..66447
                     /locus_tag="VW325_000207"
     CDS             65857..66447
                     /locus_tag="VW325_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9733683.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            66456..68219
                     /locus_tag="VW325_000208"
     CDS             66456..68219
                     /locus_tag="VW325_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9733684.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINFLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFEFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASFFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT        20658 a        12409 c        15340 g        19999 t
ORIGIN      
        1 tttttatcaa aaaatacttt acaagttctt aaaaacatga tatagtaata aagcttagaa
       61 aatgagatga tgttttctag caaatataaa cccgagtaaa aaatgcctac ggacaggcag
      121 ggttgaatgc cgaagcgtgg ttgaaaagcc acattattga tagggttaaa agcctacttt
      181 tataagttga tgttaggaca cttgtcctaa ttcataaatt tttagtgtgg tgaaagcaca
      241 cgtcatcttg tgaaacgatc aataaagtac gtaatatttg ctactagaga gttaggaaac
      301 atcgggaaca gacatactca acagaaacca aaataaacac gtcagaagat tgcagagcag
      361 gtgaaaacct gctctttttt catgagtcaa cctttagttc cttagttttc ataaggtcct
      421 aaaaatattg aaaggagtat gttttgaaag agttagatca aaaccaagcc ccaatttatg
      481 aggccttggt gaagttgcgc aagaaaagga ttgttccctt tgatgttcca ggtcacaagc
      541 gtggacgggg aaatccagaa cttgtcgaac tcttaggaga aaaatgtgta ggcattgatg
      601 tcaattcgat gaaacccttg gataatttag gccatcctat ttcgattatt cgtgatgcag
      661 aggagctggc tgcagatgct tttggagcta gccatgcctt tctaatgatt ggtggaacaa
      721 cttcatcggt gcagactatg attctggcaa cctgcaaggc aggagataag attattctgc
      781 cacgaaatgt ccataaatct gctatcaatg cgttggttct atgtggtgcc attcccatct
      841 atatcgagat gagtgtagat cctaagattg gtatcgcttt aggtcttgaa aatgaccgag
      901 tagcacaggc cataaaggac catccagatg ctaaggctat cctaatcaac aatcctactt
      961 actacggcat ctgttcagac ctaaaggggt tgacagaaat ggctcatgaa gctggcatga
     1021 tggttttagt agatgaagcc cacggagcgc atttgcattt cactgataaa cttccaattt
     1081 ctgctatgga tgcaggggct gatatggcag cagtttccat gcataagtct ggtgggagtt
     1141 tgacccaaag ctccatttta cttatcgggg agcagatgaa ttctgaatac gttcgtcaga
     1201 taattaacct gacccagtct acatctgcct cttacttgtt gatggctagt ttggatattt
     1261 cacgtcgcaa cttggccctt cgtggtaaag agtcgtttga ggaagtcatt gagctatctg
     1321 agtatgcccg ccgtgaaatc aatgctatcg gtggctacta tgcctactca aaagagttaa
     1381 tagacggtgt ttcggtttgc gattttgacg taactaagtt gtcagtttac actcagggta
     1441 ttggcttaac aggtatcgag gtttatgacc tcttgcgaga cgaatacgac attcagatcg
     1501 agtttggtga tatcggcaat atcttggcct atatttccat cggcgaccgc atccaagaca
     1561 tcgagcgctt ggttggtgct ctggctgata ttaagagact ctattcaaga gatggaaaag
     1621 atttgatagc aggagaatat attcagcccg agttagtgct gtctccgcaa gaagccttct
     1681 attcagaaag aaaaagttta actttggatg attctgttgg acaggtctgt ggagaatttg
     1741 ttatgtgtta ccctccaggt attcctatct tggctcctgg tgaacgcatt acacgagaaa
     1801 ttgtcgacta tatccaattc gccaaggaac gtggttgctc cctccaaggg acggaagatc
     1861 cagaggtcaa tcatatcaac gttattaaga gaaagacaaa ctataagaaa agtcaatagt
     1921 ttatctaaac tatttcttat ttcaatttga tgatttggcg atgattttag agcacggcaa
     1981 aaagcccttg aattagaagc ggtcaatcgc ttaatttcta tcagcttatc aaatcctgcc
     2041 tcaatccttt tctgaggatt agggtagcgt gtcaagagtt ggtaggtata ttctgaatgc
     2101 tttccaacga ttttatccaa ctcaggaaag atgatatcaa ggcaacgagt gtattgtact
     2161 ttccaatcag actgtttttt cttgagacga tgaatatgtc tagtcagtat ttttagttct
     2221 acttgccgat tattgtgttg aaattgttca cgattggggt cagaaagaag tttaagagcg
     2281 atgccatgag cgtctttctt atccgtttta gtcttgcgaa gtgataatga tttggcaaat
     2341 ttcttgatga gcaaaggatt gtaggtgtaa actttatatc cttgttcatg caggaagttc
     2401 agtagattaa aggcataatg tccagtatct tcaagagcga tgagacagtc ttggttgagc
     2461 tgtcgaagag acagatctaa gagttcaaaa ccagctttat tatttgaaaa agtgagtggt
     2521 ttaagaacag tttttccttg aacattcaag gctgtaacat cgtgtttatt tttagcgaca
     2581 tcaatgccca cataaggcat gggagtacct ccagatgtag tatttcaagt ctacttggtt
     2641 atccacgaac tttttgccct gttaccttag acgagataaa acgtctatgc gttatcaaac
     2701 tcattaccaa ttgaaacaaa aagctgtggt tagagccttt aggaaatcgt caagcgattg
     2761 gaggaaatga actaatccac agtggcttat tccaagtata ccacttgggc tttggcagta
     2821 gctaactgcg ctaaatataa tataaggaga aatagatgga tttatggttt tctgaagttc
     2881 atactccaga tgtcaaattg tctctgagaa cagccaagca actttacgct ggaaaaagtg
     2941 aatggcagga tatcgaagtc ttggatacgc cagcttttgg gaaaatactg attttaaatg
     3001 gccatgtctt gttctcagat gcggatgatt tcgtctacaa tgaaatgacc gtccacgttc
     3061 ccatggctgt ccacccaaat ccaaagaaag tattggttat tgggggtggt gacggcggtg
     3121 ttgcccaagt attaacactc tatcctgaac tggaacaaat cgatatcgtg gaaccggatg
     3181 agatgttggt cgaggtctgt cgtgagtatt tcccagactt tgctgcaggc ctagatgatc
     3241 ctcgtgttac tatttactac caaaatgggc tacgcttttt gcgaaactgc gaagatgact
     3301 atgatattat catcaacgat gcgacagatc catttggcca tacggaagga ctctttacca
     3361 aggaattcta cggcaatagt tatcgagctc tgaaggaaga cggcatcatg atttaccagc
     3421 atgggagtcc cttctttgac gaggatgagt cggcctgccg aagcatgcac cgcaaggtca
     3481 atcaagcctt tccaatcagt cgggtttatc aggcccatat tccaactagc ccagctggct
     3541 attggttgtt tggatttgca tcgaaaaaat accaccctgt caaagatttt gacaaggaag
     3601 gctggaaaaa acgccagctt ttcacagaat actacactgc aaacttacac gtgggagcct
     3661 ttatgttgcc caagtatgtt gaggacattt tagaagaaga ggaaggaaaa aaatgagtcg
     3721 tttactagtt attggttgtg ggggcgttgc ccaagttgct atttcaaaga tttgtcaaga
     3781 tagcgaaaca tttacagaga ttatgattgc tagccgtacc aagtcaaaat gcgatgactt
     3841 gaaagcgaag ctagaaggca aaacaagtac taaaattgaa actgcagcac ttgatgctga
     3901 caaggttgaa gaagtgattg ccctgattga aagctacaaa ccagaagctg ttttgaatgt
     3961 agctctgcct tatcaagatt taaccattat ggatgcttgt ttggcaacag gtgttcacta
     4021 tatcgataca gccaactacg aagcagaaga cacagaagac cctgagtggc gtgctatcta
     4081 cgaaaaacgt tgtaaggaac ttggttttac agcctacttt gactactcat ggcagtgggc
     4141 ttatcaagag aaattcaaag aagcaggctt gactgctctt cttggttctg gttttgaccc
     4201 aggtgtaact agtgtctttt cagcttatgc cctcaaacac tattttgatg aaatccatta
     4261 tatcgacatt ttagactgta atggcggtga ccacggttat ccatttgcaa ccaactttaa
     4321 tccagaaatt aatctccgtg aggtttctgc gccaggttct tactgggaag atgggaaatg
     4381 ggtcgaagtc gaagctatgt ctatcaagcg tgagtatgat ttccctcaag ttggacaaaa
     4441 agatatgtat ctccttcacc atgaagaaat cgaatcattg gccaagaaca ttccaggtgt
     4501 caaacgcatt cgtttcttta tgacttttgg tcaatcttac ttgacgcaca tgaaatgtct
     4561 tgaaaatgtt ggactccttc gtacggatac cattaacttt aacggccaag aaattgttcc
     4621 aattcaattt ttgaaagcct tgcttccaga tcctgctagc cttggcccac gtactgtagg
     4681 taaaaccaat attggatgta tctttacagg tgtcaaagat ggcgttgaaa agactatcta
     4741 catctacaat gtttgcgacc atcaagagtg ctatgcagaa gtcgggtcac aagcaatttc
     4801 atacacgaca ggcgttccag ccatgattgg gacaaaatta gtcatgaacg gaacttggaa
     4861 acaagctgga gtgtataacc ttgaggagtt agatccagat ccattcatgg aagctttgaa
     4921 tgagtatggt ttgccatggg ttgtggttga aaatccacaa atggtggact aatgaagtta
     4981 gaacaagtac caacaccagc ctatgttatt gacttggcca agttagaagc taattgccgc
     5041 attctacaat atgtacaaga agaggccggt tgcaaggtct tgcttgccca gaaggcatat
     5101 tccctctaca aaacttatcc cttgattagc cagtatctat caggtacgac agctagtgga
     5161 ctctatgagg ccaaattggc aagggaagaa tttcctggtg aagtccatgt ttttgcacca
     5221 gctttcaagg atgaggacat ggaggaattg cttgggataa cagaccatat cgtctttaac
     5281 tcagagagac agttgcgtaa gcatggggct cgttgtcgag aggctggtgt cagtgttggt
     5341 ttgcgcctca accctcagtg ttcaactcaa ggagatcacg cgctctatga cccttgtgca
     5401 cctggttctc gctttggagt tactatagac aagattccga gtgatttgct agatttggtt
     5461 gacggacttc attttcatac cctttgcgag cagggagcag atgatttaca aacaactttg
     5521 aaagcagtag aagaacagtt tggtccctac ttacatgagg taaaatggct caatatgggt
     5581 ggtggtcatc atattacaag agaaggttac gatgtggatt tgctgatttc agaaatcaag
     5641 cgtatccgaa aaacttacaa tcttgaaatc tatatcgagc ctggtgaagc cattgcgctt
     5701 aatgcgggtt atttagcaac tgaggtatta gatattgtag aaaacggtat ggaaatcttg
     5761 gttttagacg cctctgcgac ctgccatatg cctgatgtac ttgagatgcc ctatcgtcca
     5821 cctttgagaa atggctttga gtcacaggaa aaagcccata cctacagact ttcttctaat
     5881 acctgtctga ctggcgatgt gattggtgat tatagttttg aaaatccagt ccaaatcgga
     5941 gacagacttt attttcaaga catggccatt tattcttttg tcaaaaataa tacctttaat
     6001 ggtattggat tgccaagtct ctatctcatg gacgaacagg gagactgtag cttactcaaa
     6061 gcttttggct atcaagactt taaagggaga ttatcatgat ggacagtcca aaaaaattag
     6121 gctatcacat gccagcagag tacgaacccc atcatggtac cctcatgata tggccgactc
     6181 gaccaggatc atggcctttt caaggaaagg ctgctaaaag agcatttact cagatcatcg
     6241 agaccatagc agaaggggaa agagtctatc ttttggtgga gcaggcctat ctatctgaag
     6301 cccaatccta tcttggagac aaggttgttt atttagacat tcccaccaat gatgcctggg
     6361 cgcgtgatac tggcccaacc attctcgtca atgataaagg taagaaatta gccgtggatt
     6421 gggcctttaa tgcttgggga ggcacctatg atggtcttta tcaagattat gaagaggatg
     6481 accaagtagc cagtcgtttt gctgaggcct tggaaaggcc tgtctatgat gctaaacctt
     6541 ttgtactgga aggaggcgca atccatagcg atggtcaagg aactattctc gtaactgaaa
     6601 gttgcttgct tagtcctggt cgcaatccta acctgactaa agaggagatt gaaaacacat
     6661 tattagaaag tcttggtgct gaaaaagtta tttggcttcc ttatggtatt tatcaggatg
     6721 aaaccaatga acacgtcgat aatgttgttg cctttgttgg tcctgctgag gttgttttgg
     6781 cttggacaga tgacgaaaat gatccccagt atgccatgtc aaaagcagat ctcgaactct
     6841 tagaacagga aacagatgca aaaggttgtc acttcaccat tcataaattg cctatccctg
     6901 cagttcgaca agttgtgaca gaagaagatt tgccaggcta catctatgaa gaaggagaag
     6961 aagagcgata cgcaggtgaa cgactagcag cttcctacgt aaacttttat atcgccaaca
     7021 aggctgtctt ggttccacag tttgaggatg taaacgacca agtggcctta gatatcctca
     7081 gcaagtgttt cccagaccgt aaagttgtcg gaataccagc cagagatatt ctcttaggtg
     7141 gtggcaatat ccactgtatc acccaacaaa ttccagaata ggagaaaaag atgagaaatg
     7201 taagagttgc aaccattcag atgcaatgcg ctaaggatgt ggcaacaaat atccaaaccg
     7261 cagagcgttt agtacgtcag gctgctgagc aaggagccca aattattctc ttgcccgagt
     7321 tgtttgaaca tccctatttc tgtcaggaac gtcagtatga ctactaccag tatgcccaat
     7381 ctgtagcgga aaatactgcc attcagcatt ttaaggtgat tgctaaggaa ctacaagttg
     7441 ttttaccaat cagtttctat gaaaaagatg gtaatgtctt gtataactct attgccgtca
     7501 ttgatgcaga tgggggagtg ctgggcgttt atcgaaagac ccatatacca gatgaccatt
     7561 attatcaaga aaaattctat ttcacgcctg gtaacactgg tttcaaggtc tggaatactc
     7621 gctatgctaa gattggtatc ggtatctgtt gggatcaatg gttccctgaa acagcccgct
     7681 gtcttgcatt gaatggtgct gaattgctct tttatcctac agctatcggt tcagagccaa
     7741 ttttggatac agatagttgt ggtcactggc aacgtactat gcaagggcac gcagcagcga
     7801 atattgttcc agtcatcgca gccaatcgtt atggtttaga ggaggttact cctagtgagg
     7861 aaaatggcgg acagagttcc agtcttgact tctacggttc ctcctttatg acggatgaaa
     7921 caggagctat tctagaacga gctgaaagac aagaagaagc tgttctgtta gctacttatg
     7981 acctagacaa gggagcaagt gaacgtctaa actggggctt gtttcgagat agaagaccag
     8041 aaatgtatag acaaattaca gattagtgtg ggagaaatga gagattcatt ctgctagact
     8101 aacttcttat tagcaactat aagatactat ggcatctagt aaatcgattt ttatgattcg
     8161 ctattcttgt ctattgatta gtccgtattt taaaatatta gcaaaaaagc aaatagcagt
     8221 aacttctgtc tatttgcttt tcttttttat agaatatatt tctcaatagc acgcgcaacg
     8281 ccgtcttctt cgttgcttga ggtaacggca tccgcaagag atttgacata atcgctggca
     8341 tttcccattg caatcccaag ccctgcaaac tggagcattt cgatatcgtt attagcatcg
     8401 cccatggcca taatctctga ggaatcaatc ttcaaaatct cagctagtcg tgaaagagca
     8461 gtagcctttg tcgttccaag cggcattgct tcataaatga caggctgcga acgaactcca
     8521 ctgaatcgtt ggcaaagctc ttcagcaaaa cgctgctcaa aatcgtctgt ttgttctttt
     8581 gttcctaaaa acataccttg gaacatccgg aactttccac tagtcgcttc ttcaagagaa
     8641 atttcagtca ggtctgaaaa tactagttta gcatcatttt caataacttg attgggtttg
     8701 ccaccgagaa caaaataatg tgactcgtca aaaagtgtca actgaacatc actcttttca
     8761 gcaaggtcat agaggtattc gatgtcagct ggactcagtt ctttccagtc aactagactc
     8821 caatcactgg tctggtgagt tgaacaaccg ttgttaacaa taatatattc gttctggagg
     8881 tcaagctcca gttttttgta gtaggggagg acaccgaaaa gggggcgacc cgtacagaga
     8941 accagtttga cacctttttc aatggctttg tgaatagcag taatgtgagc ttgtgggatt
     9001 tccttggctt cattgaggag ggtgccgtcc atatccaagg ctagtagttt aatcataggt
     9061 cttccttctt tatcttttgc tattattata gcatattttg gagaagaaat tgatagaaag
     9121 cttgagacta attgatttta tagtttaaga tgttttgatg acaattcatg atttgaagag
     9181 gatatttggc aaagatatgc tatactatgt ttgtcaatgt tgcaactaga caaattaaaa
     9241 aaccaactta atataatagt ttttttgtaa gtaggtatga gtatcagatt actcaactaa
     9301 tctgaagaat aatggaggaa atatatcatg attttaatga caaaaaatat aaatctaaca
     9361 aatgaagaat tagagctgat acaaggtgga gcagattcat atggtaaaaa tcctaatggt
     9421 aggtacgatt gggaaataga accagtatta actctgccgg ttcatggatt ttgttccaga
     9481 ggcacctatg atttaggata tattggagga ggcaatcatc tttgcaaagg aagtgctgcg
     9541 agattttaag taaaatttat tagtaatatg aagaaacaag tggagaaagc agagaattta
     9601 atatgaaaaa acgggctatt caaattttac tagcattgtc cttaattttt tacaaatcaa
     9661 cttggttttg gagagttttc aatcacctcg caaagcccta tctaccagca agtcgtgaat
     9721 tttttcagat tctgcttttg atggagagcg gagttctttt cttagcggtc atctatctac
     9781 tggtttttgc aggaaagaaa atttttcatt tcaagtggca gctgaggtac ttcatctacc
     9841 ttttactggg ctacatcatt tcatatatgt ctgacttcct cttttcgtat ttcatatccc
     9901 tgtcttcaaa tcagatttct ttgaatgaaa cgatagaaat gatggggaga caggagttcc
     9961 cttatttctt gctcatcgtt tgcttcatcg cccctattgc tgaggaattg atttatcgag
    10021 gtgtgcttat gacaaccttt ttcaaaaact caccttggta cggagatgtt ttgctttctg
    10081 ctattatttt cggttatatt catatcaatt ttgctttaac tcctcttgct tttttcattt
    10141 atgctagtgg aggtctgatt ttagctctat tgtatcgcat gactaaaaat ctctactatc
    10201 caatactagt tcatattctc atcaatatca ctgccttctg ggatgtttgg ttactcctat
    10261 tttcaggaag ttagcttact aaaaaaatgt cggaattttc cggcattttc ttttttcaca
    10321 aatagtcaac gtttttcttt ccgatactga agtggtgtgt agccacttat ttttttgaat
    10381 tgattttgaa aataagattg gcgtgagaaa ggcagatagt gaagatagtt aagaagaata
    10441 ggatgttctt ttttcctttt tggaaaactt ctaaaatatg gtataatgaa aagataaaga
    10501 agttgggggt agaagatgaa cattcaacaa ttacgctatg ttgtggctat tgccaatagt
    10561 ggtacttttc gtgaagctgc tgaaaagatg tatgttagtc agccgagtct gtctatttct
    10621 gttcgtgatt tggaaaaaga gttgggcttt aagattttcc gtcggaccag ctcagggact
    10681 ttcttgaccc gtcgtgggat ggaattttat gaaaaagcgc aagaattggt taaaggattt
    10741 gatatttttc aaaatcagta tgccaatcct gaagaagaaa aagatgaatt ttccgttgct
    10801 agccagcact atgacttctt accaccaact attacggcct tttcagagcg ctatcctgac
    10861 tataagaact tccgtatttt tgaatcaact actgttcaaa tattagatga agtggcgcaa
    10921 gggcatagtg agattgggat tatctacctc aacaatcaaa ataaaaaggg gattatgcaa
    10981 cgggttgaaa agttaggtct ggaggtcatc gaattgattc ctttccatac ccatatttat
    11041 ctctgtgagg gtcatccttt agcccagaaa gaggaattag tcatggagga tttagcggat
    11101 ttaccaacgg ttcgtttcac tcaagagaaa gacgagtacc tttattattc agagaacttt
    11161 gtcgatacca gcgctagctc acagatgttt aatgtgacag accgtgccac cttgaatggt
    11221 attttggagc ggacggacgc ctatgcgaca ggttctggat ttttagatag tgacagtgtt
    11281 aatggcatta cagttattcg tctcaaggat aacctagata accgcatggt ctatgttaaa
    11341 cgtgaagaag tggagcttag tcaagctggg actctcttcg tagaagtcat gcaagaatat
    11401 tttgatcaaa agaggaaatc atgaaaaaaa gagcaatagt ggcagtcatt gtactgcttt
    11461 taattgggct ggatcagttg gtcaaatctt atatcgtcca gcagattcca ctgggtgaag
    11521 tgcgctcctg gatccccaat ttcgttagct tgacctacct gcaaaatcga ggtgcagcct
    11581 tttctatctt acaagatcag cagttgttat tcgctgtcat tactctggtt gtcgtgatag
    11641 gtgccatttg gtatttacat aaacacatgg aggactcatt ctggatggtc ttgggtttga
    11701 ctctaataat cgcgggtggt cttggaaact ttattgacag ggtcagtcag ggctttgttg
    11761 tggatatgtt ccaccttgac tttatcaact ttgcaatttt caatgtggca gatagctatc
    11821 tgacggttgg agtgattatt ttattgattg caatgctaaa agaggaaata aatggaaatt
    11881 aaaattgaaa ctggtggtct gcgtttggat aaggctttgt cagatttgtc agaattatca
    11941 cgtagtctcg cgaatgaaca aattaaatca ggccaggtct tggtcaatgg tcaagtcaag
    12001 aaagctaaat acacagtcca agagggtgat gtcgtcactt accatgtgcc agaaccagag
    12061 gtattagagt atgtggctga ggatcttccg ctagaaatag tctaccaaga tgaggatgtg
    12121 gctgtcgtta acaaacctca gggaatggtt gtgcacccga gtgctggtca taccagtgga
    12181 accctagtaa atgccctcat gtatcatatt aaggacttgt cgggtatcaa tggggttctg
    12241 cgtccaggga ttgttcaccg tattgataag gatacgtcag gtcttctcat gattgctaaa
    12301 aacgatgatg cgcatctagt acttgcccaa gaactcaaag ataaaaagtc tctccgcaaa
    12361 tattgggcga ttgttcatgg aaatctgcct aatgatcgtg gtgtaattga agcgccgatt
    12421 ggccggagtg aaaaagaccg taagaaacag gctgtaactg ctaaagggaa gcctgcagtg
    12481 acgcgttttc acgtcttgga acgctttggc gattatagct tagtagagtt gcaactggag
    12541 acagggcgca ctcatcaaat ccgtgtccac atggcttata tcggccattc agtcgctggt
    12601 gatgaggtct atggtcctcg caagactttg aaaggacatg gacaatttct tcatgccaag
    12661 actttaggtt ttactcatcc gagaacaggt aagaccttgg aatttaaagc agatatccca
    12721 gagattttta aggaaacctt ggagagattg agaaagtaag aatgaaaaag aaattaacta
    12781 gtttagcact tgtaggcgct tttttaggtt tgtcatggta tgggaatgtt caggctcaag
    12841 aaagttcagg aaataaaatc cactttatca atgttcaaga aggtggcagt gatgcgatta
    12901 ttcttgaaag caatggacat tttgccatgg tggatacagg agaagattat gatttcccag
    12961 atggaagtga ttctcgctat ccatggagag aaggaattga aacgtcttat aagcatgttc
    13021 taacagaccg tgtctttcgt cgtttgaagg aattgggtgt ccaaaaactt gattttattt
    13081 tggtgaccca tacccacagt gatcatattg gaaatgttga tgaattactg tctacctatc
    13141 cagttgaccg agtctatctt aagaaatata gtgatagtcg tattactaat tctgaacgtc
    13201 tatgggataa tctgtatggc tatgataagg ttttacagac tgctgcagaa aaaggtgttt
    13261 cagttattca aaatatcaca caaggggatg ctcattttca gtttggggac atggatattc
    13321 agctctataa ttatgaaaat gaaactgatt catcgggtga attaaagaaa atttgggatg
    13381 acaattccaa ttccttgatt agcgtggtga aagtcaatgg caagaaaatt taccttgggg
    13441 gcgatttaga taatgttcat ggagcagaag acaagtatgg tcctctcatt ggaaaagttg
    13501 atttgatgaa gtttaatcat caccatgata ccaacaaatc aaataccaag gatttcatta
    13561 aaaatttgag tccgagtttg attgttcaaa cttcggatag tctaccttgg aaaaatggtg
    13621 ttgatagtga gtatgttaat tggctcaaag aacgaggaat tgagagaatc aatgcagcca
    13681 gcaaagacta tgatgcaaca gtttttgata ttcgacaaga cggtttagta aacatttcaa
    13741 cttcctacaa gccgattcca agttttcaag ctggttggca taagagttca tatggaaact
    13801 ggtggtatca agcgcctgat tctacaggag aatatgctgt cggttggaat gaaattaagg
    13861 gtgaatggta ttactttaac caaacgggta tcttattaca gaatcaatgg aaaaaatgga
    13921 acaatcgttg gttctatttg acagactctg gtgttgctgc taaaaattgg aagaaaatcg
    13981 ctggaatctg gtattatttt aacaaagaaa accagatgga aattggttgg attcaagata
    14041 aagagcagtg gtattatttg gatgttgatg gctctatgaa gacaggttgg cttcaatata
    14101 aggggcaatg gtattacttt gctccatcag gggaaatgaa aacgggctgg gtaaaagata
    14161 aagaagcctg gtactatatg gattctactg gtatcatgaa gacaggtgag atagaagttg
    14221 ctggacatca ttactatcta gaagaatcag gagctatgaa gcaaggctgg cttaaaaagg
    14281 caaatgattg gtatttctac aagacagatg gttcacgagc tataggttgg attaaagaca
    14341 aggataaatg gtacttcttg aaagaaaatg gtcaattact tgtgaatggt aagacaccag
    14401 aaggctatac tgtggattca agtggtgcct ggttagtgga tgttccgatc gagaaatctg
    14461 ctacaattaa aactacaagt cattcagaaa taaaagaatc caaagaagta gtgaaaaagg
    14521 atcttgaaaa taaagaaacg agtcaacatg aaagtgttac aaattcttca actagtcaag
    14581 atttgacatc ctcaacttca caaagctctg aaacgagtgt aaacaaatcg gaatcagaac
    14641 agtagtagaa aagaaggttt tagggccttc tttttcctat caactctttt ctatttcctg
    14701 ttattcatgt tataatggat aaatatgaat aatcggagtg agactatgaa atacaaacgg
    14761 attgtcttta aggtgggtac ttcttctctg acaaatgagg atggaagttt atcacgtagt
    14821 aaggtaaagg atattaccca gcagttggct atgctgcacg aggctggtca tgagttgatt
    14881 ttggtgtctt caggtgccat tgcggctggt tttggagcct taggatttaa aaagcgtccg
    14941 actaaaattg ctgataaaca ggcttcagca gcggtagggc agggactttt gttggaagaa
    15001 tatacgacca atcttctctt gcgtcaaatc gtttctgcac aaatcttgct gacccaagat
    15061 gactttgtgg ataagcgtcg ttataaaaat gcccatcagg ctttgtcggt tttgctcaac
    15121 cgtggggcaa ttcctatcat caatgagaat gatagtgtcg ttattgatga gctcaaggtt
    15181 ggggacaatg acactctaag tgctcaagta gcggcgatgg tccaagcaga ccttttagtt
    15241 ttcttgacag atgtggacgg tctctatact ggaaatccta attcagatcc aagagccaaa
    15301 cgcttggaga gaatcgagac catcaatcgt gagattattg atatggctgg tggagctggt
    15361 tcgtcaaacg gaactggggg tatgttaacc aaaatcaagg ctgcaactat cgcgacggaa
    15421 tcaggagttc ctgtttatat ctgctcatcc ttgaaatcag attccatgat tgaggcggca
    15481 gaggagaccg aggatggttc ttactttgtt gctcaagaga aggggcttcg tacccagaaa
    15541 caatggcttg ccttctatgc tcagagtcaa ggttctattt gggttgataa aggggctgcg
    15601 gaagctctct ctcaacatgg aaagagtctt ctcttatctg gtatcgttga agcagaagga
    15661 gtcttttctt acggtgatat cgtgacagta tttgataagg aaagtggaaa atcacttgga
    15721 aaaggacgcg tgcaatttgg agcatctgct ttggaggata tgttgcgttc tcaaaaagcc
    15781 aagggtgtct tgatttaccg tgacgactgg atttccatta ctcctgaaat ccaactactt
    15841 tttacagaat tttagaggta aactatggtg agtagacaag aacaatttga acaggtacag
    15901 gctgttaaaa aatcgattaa cacagctagt gaagaagtga aaaaccaagc cttgctagcc
    15961 atggctgatc acttagtggc tgctactgag gaaattttag cggctaatgc cctcgatatg
    16021 gcagcggcta aggggaaaat ctcagatgtg atgttggatc gtctttattt ggatgcagat
    16081 cgtatagaag cgatggcaag aggaattcgt gaagtggttg ccttaccaga tccaatcggt
    16141 gaagttttag aaacaagtca gcttgaaaat ggtttggtta tcacaaaaaa acgtgtagct
    16201 atgggtgtca tcggtattat ctatgaaagc cgtccaaatg tgacgtctga tgcggctgct
    16261 ttgactctta agagtggaaa tgcggttgtt cttcgtagtg gtaaggatgc ctatcaaaca
    16321 acccatgcca ttgtcacagc cttgaagaag ggcttggaga cgactactat tcatccaaat
    16381 gtgattcaac tggtggagga tactagccgt gaaagtagtt atgctatgat gaaggccaag
    16441 ggctatctag accttctcat tcctcgtgga ggagctggct tgatcaatgc agtggttgag
    16501 aatgcgattg tacctgttat cgagacaggg actgggattg tccatgtcta tgtggataag
    16561 gatgcagacg aagacaaggc gctgtctatc atcaacaatg ctaaaaccag tcgtccttct
    16621 gtttgtaatg ccatggaggt tctgctggtt catgaaaaca aggcagcaag catccttcct
    16681 cgcttggatc aaatgttggt tgcagatcga aaagaagctg ggttggaacc aattcaattc
    16741 cgcctagata gcaaagcaag ccagtttgtt tcaggtcaag ctgctcaagc acaagacttt
    16801 gataccgagt ttttagacta tattctagct gttaaggttg tgagcagttt agaagaagcg
    16861 gttgcgcata ttgaatccca cagtacccat cattcggatg ctattgtgac ggaaaatgct
    16921 gaagctgcag catactttac agatcaagtg gactctgcag cggtgtatgt taatgcctca
    16981 actcgtttca cagatggagg acaatttggt cttggttgtg aaatggggat ttctactcag
    17041 aaattgcacg cgcgtggtcc aatgggcttg aaagagttga ccagctacaa gtatgtggtt
    17101 gctggtgatg ggcagataag ggagtaagag atgaagattg gatttatcgg tttggggaat
    17161 atgggtgcta gcttggcaaa atctgtcttg cagactagga cgtcagatga gattctcctt
    17221 gccaatcgta gtcaagctaa ggtagatgct ttcattgcag actttggtgg tcaggcttcc
    17281 agcaatgaag aaatgtttgc agaagcagat gtgatttttc taggagttaa gcctgctcag
    17341 ttttctgaac tgctttctca atatcagacc atccttgaaa aaagagaaag tcttcttttg
    17401 atttcgatgg cagctggatt gaccttagaa aaactagcaa gtcttatccc aagccaacac
    17461 cgaattattc gtatgatgcc taatacccct gcttctatcg ggcaaggagt gattagttat
    17521 gccttgtctc ctaattgcag ggctgaggac agtgagatct tttgtcagct tttagccaag
    17581 gctggtctct tggttgaatt aggagaaagc ttaatcaatg cagcgacagg tcttgcaggt
    17641 tgtggaccag cctttgtcta tctctttatt gaggccttgg cagatgcagg tgttcagacg
    17701 ggattaccac gagaaacagc cttgaaaatg gcagcccaaa ctgtggtagg agctgggcaa
    17761 ttggtcctag aaagccagca acatcctgga gttttgaaag atcaagtttg tagtccaggc
    17821 ggttcgacta ttgctggcgt agcaagccta gaagcgcatg cttttcgagg cacagtcatg
    17881 gaggcagttc atcaagccta taaacgaaca caagaactag gtaaataaga ggtagtttta
    17941 tctgcctctt ttatggtggt tgatactctt cgaaaatcaa attcaaactg cgtcagcttc
    18001 accttgccgt actcaagtac agcctgcggc tagcttccta gtttgctctt tgattttcat
    18061 tgagtatgaa atgagaagac aaaaagattg tcacaaaccc ctattttttt gatagaatag
    18121 aagtagtaaa aaagaaatga gttagacatg tcaaaaggat ttttagtctc tcttgaggga
    18181 ccagagggag caggcaagac cagtgtttta gaggctctgc taccaatttt aaaggaaaaa
    18241 ggagtagagg tgttgacgac ccgtgaacct ggcggagtct tgattgggga gaagattcgg
    18301 gaagtgattt tggatccaag tcatactcag atggatgcta aaacagagct acttctctat
    18361 attgccagtc gcagacagca tttggtggaa aaagttcttc cagcccttga agctggcaag
    18421 ttggtcatca tggatcgttt tatcgatagt tctgttgcct atcagggatt tggtcgtggc
    18481 ttagatattg aagccattga ctggctcaat cagtttgcga cagatggcct caaacccgat
    18541 ttgacactct attttgacat cgaggtggaa gaagggctgg ctcgtattgc tgcgaatagt
    18601 gaccgcgagg ttaatcgttt ggatttggaa gggttggact tgcataaaaa agttcgtcaa
    18661 ggctaccttt ctcttctgga taaagaggga aatcgcattg tcaagattga tgctagtctc
    18721 cctttggagc aagttgtgga aactaccaag gctgtcttgt ttgacggaat gggcttggcc
    18781 aaatgaaaca agatcaacta aaggcttggc aaccagctca gtttgaccgt tttgtccgta
    18841 tcttagaaca agaccagctc aatcacgcct atctcttttc aggtttcttt ggaagcttgg
    18901 aaatggcgca atttttagct aagagcctct tttgtacgga taaagttggc gtcttaccat
    18961 gtgagaaatg ccgaagttgc aagctgattg aacaggaaga gtttccagat gtcaccttga
    19021 ttaagccagt caatcaggtc atcaagacag aacgcattcg ggaattggtg ggacagtttt
    19081 ctcaagcagg gattgaaagc cagcaacagg tctttattat cgagcaagcg gataaaatgc
    19141 atcccaacgc agccaattct ctgctcaagg tcatcgaaga accccagagt gaagtttata
    19201 ttttcttctt gactagcgat gaggaaaaga tgttaccgac aatccgaagt cggactcaga
    19261 tcttccactt taaaaagcaa gaagaaaaac ttatcttact cttagaacaa atgggacttg
    19321 ttaagaaaaa agcgactctt ttagctaagt ttagtcaatc gcgagctgaa gcagaaaagt
    19381 tggctaatca ggcaagtttt tggaccttgg tcgatgaaag tgaacgcctg cagacttggt
    19441 tagtagctaa gaaaaaagaa agttatctac aggttgccaa attagccaac ttggcagatg
    19501 ataaggaaaa acaggatcag gttttacgga ttcttgaagt tctctgtggg caggacctct
    19561 tgcaggtaag agtaagagtg attctacaag atttactaga agctagaaaa atgtggcaag
    19621 ctaatgtcag ctttcaaaat gccatggaat atctggtctt gaaagaaata taaactcaaa
    19681 aatgaatgat aaagaaagga aagggctgtt ttatggacaa aaaagaatta tttgacgcgc
    19741 tggatgattt ttcccaacaa ttattggtaa ccttagccga tgtggaagcc atcaagaaaa
    19801 atctcaagag cttggtagaa gaaaatacag ctcttcgctt ggaaaatagt aagttgcgag
    19861 aacgcttggg tgaggtggaa gcagacgctc ccgtcaaggc caagcatgtt cgcgaaagtg
    19921 tccgtcgtat ttaccgtgat ggatttcacg tatgtaatga tttttatgga caacgtcgag
    19981 agcaggacga agaatgtatg ttctgtgatg agttgttata cagggagtaa gcatgcaaat
    20041 tcaaaaaagt tttaaggggc agtctcccta tggcaagctg tatctagtgg caacgccgat
    20101 tggcaatcta gatgatatga cttttcgtgc tatccagacc ttgaaagaag tggactggat
    20161 tgctgctgag gatacgcgca atacagggct tttgctcaag cattttgaca tttccaccaa
    20221 gcagatcagt tttcatgagc acaatgccaa ggaaaaaatt cctgatttga ttggtttctt
    20281 gaaagcaggg caaagtattg ctcaggtctc tgatgccggt ttgcctagca tttcagaccc
    20341 tggtcatgat ttggttaagg cagctattga ggaagaaatt gcagttgtta cagttccagg
    20401 tgcctctgca ggaatttccg ccttgattgc cagtggttta gcgccgcagc cacatatctt
    20461 ttacggcttt ttaccgagaa aatcaggcca gcaaaagcaa tttttcgact cgaaaaaaga
    20521 ctatcctgaa actcagattt tttatgaatc gccccatcgt gtggcagata cattggaaaa
    20581 tatgctagaa gtctacggtg accgctccgt tgtcttggtc agggaattga ccaaaatcta
    20641 tgaagaatac caaagaggta caatttctga attgctggaa agcattgctg aaacgtctct
    20701 caagggtgaa tgtcttctga ttgttgaagg tgccagtcag gatgtggaag aaaaaggcga
    20761 ggaagacttg ttcttagaaa tccaagctcg tattcagcaa ggtatgaaga aaaatcaagc
    20821 tattaaggaa gtagctaaga tttaccagtg gaataagagt caactctacg ctgcctacca
    20881 cgactgggaa gaaaaacaat aaagggagac aggatgtaat aattctgtct gtttctgttt
    20941 aacttaatta gtgatgataa tataaagatg tatcacttgg tatagaagct ttggtattaa
    21001 gttttttatt aagcccatac ggaataccga tggttggagc agcagttata gcgttcttag
    21061 aaggtataaa tagaaaaata aggtcatttt aaatcaaagg attgataaat cagaaagaag
    21121 gtgatttttt gcgaacatac gaaaataaag aagaactaaa agctgagata gagaaaacat
    21181 ttgagaaata tattttagaa tttgataata ttccagaaaa tttaaaagat aagagagctg
    21241 atgaagttga cagaactcca gcagaaaacc ttgcttatca ggttggttgg accaacttgg
    21301 ttcttaaatg ggaagaagat gaaagaaagg ggcttcaagt aaaaacacca tcggataaat
    21361 ttaaatggaa tcaacttggt gaattatatc agtggttcac agatacctac gctcatttat
    21421 ctctgcaaga gttgaaagca aaattaaatg aaaatattca ttctatctct gcaatgattg
    21481 attcgttgag tgaggaagaa ttatttgaac cgcatatgag aaagtgggct gatgaagcga
    21541 ctaaaacagc gacttgggaa gtgtataagt ttattcatgt aaatacggtt gcaccttttg
    21601 gaactttcag aactaaaatc agaaaatgga agaagatagt attataaatt atatttttaa
    21661 ctttaaaaaa tttcataaaa atggttacca aaggcgatag aagaaaaact atcgtctttt
    21721 tctttgcaaa tttttaagaa gggaggtgat cttgcatgga ctttgaatat ttttataaca
    21781 gagaagcgaa aagatttaac ttcttaaaag taccggagat attagttgat agagaagaat
    21841 ttcggggctt atcagcagaa gcaattatcc tttattccat acttcttaaa tagacaggaa
    21901 tgtcatttaa gaataactgg atagacaagg aaggcagagt atttatctat tttactgtcg
    21961 aagaaattat gaaaagaaga aatatctcaa agccaactgc cataaaaaca ttagatgagc
    22021 ttgatataaa aaaaggaata ggactgatcg aaagagtaag gcttggactt ggtaagccga
    22081 acatcattta tgttaaagac tttatgagta tatttcaggt aaaagaaaat gacttacaga
    22141 agtcaaaaaa cttaacttca gaagtaaaag attttaacct cagaagtaaa gaaaatgaac
    22201 ttcaagaggt taagaacctt gactctaact atatagagaa taataagagt aagtatagta
    22261 agagagaata tagttttggt gaaaacggac ttggaacatt tcaaaatgtg tttttagctg
    22321 ctgaagatat ataggattta caaatcataa tgaactcaca gcttgagaat tacattagac
    22381 ttcctgcaaa actagaatcc tagttcatga ttgataatac cagcaatcaa attcattcgt
    22441 aatccgaatc gtttacgatg atttcgatag gttgttgaaa acattttaaa cgtttctact
    22501 ttggcaaaga tgttctcaac cttgcttctc tccttagata gcgcatggtt ataggtttta
    22561 tcttcaactg ttagcggctt gaatttgctg gatttacgtg gagtttgtgc ttgaggatat
    22621 atcttcatga gcccttgata accactgtca gccaagattt taccagcttg tccgatattt
    22681 ctgcgactca ttttgaacaa cttcatatca tgacaatagt tcacagtgat atccaaagaa
    22741 acaattctcc cttgacttgt gacaatcgct tgagccttca tagcgtagca tttcttttta
    22801 ccagaataat tcgctaattc tttttttagg gcgattgatt tttacttccg tcgcatcaat
    22861 aatcattacc gtgtcctcag aactgagagg agttcttgaa atcgtaacac cactttgaac
    22921 aagagttact tcaacccatt ggctccgacg gattaagttg ctttcgtgaa taccaaaatc
    22981 agccgcaatt tgttcataag ttcgatattc tcgcacatat tgaagagtgg ccataagaag
    23041 gtcttctagg cttaatttag gttttcgtcc accttttgcg tgtttaagtt gataagctgt
    23101 ttttaataca gctaatatct cttcaaaagt cgtgcgctga acaccaacaa gacgcttaaa
    23161 tcgtgcatca gttagttgtt tacttgcttc atcattcata gaactactat accatatttt
    23221 gtttcgcagg aagtctattg gaaagtaaga aatattgaag ctgaggcgat tagaagaaat
    23281 tgtgagcgtg gtgctatttt ttcaggtaaa ataaaacatc acgaagattc tcagtttaaa
    23341 ggagaccact atgttgaatg ttatgctgtt ttagataata cggttatagc aagagataga
    23401 ataacagtcc ctatcgatcc gttatgtgga aaagatttta tagagtagca tataattgat
    23461 tcttaactgg aatactctct atctctttac atcaagaaaa tgactaaaca gggaagtttg
    23521 ccttcttccc tttttttgtt atactagtag aagaaaaaaa tagaaagatt tgtgggagtg
    23581 aaaaagtccg ggggactttt tcagcttgag ccaagaaatt cgagagcgag gaaagtctgg
    23641 gggacttttt cagcttgagc caagaaattc gagagcgagg aaagtctggg ggactttttc
    23701 agcctgagcc aagaaactcg aaatctcgta agtctgattg gctttttcaa tgtgaatctt
    23761 aacttcacac tcccaaagag gtattagtgt cgtgtctcaa tcttatatca atgttatcgg
    23821 tgctggtttg gcaggttctg aagcagctta ccaaatcgca gagcgtggta ttccagttaa
    23881 actttatgaa atgcgtggtg tcaagtctac accccagcat aaaacagaca attttgctga
    23941 gttggtttgt tccaattctt tgcgtgggga tgctttgaca aatgcagttg gtcttctcaa
    24001 ggaagaaatg cgtcgcttgg gttctgttat cttggaatct gctgaggcta cacgtgttcc
    24061 tgccggtgga gcccttgcgg tggatcgtga tggtttctct caaatggtga ccgaaaaagt
    24121 ggtcaaccac cccttgattg aagtggtgcg tgatgaaatt acagaattgc caacagatgt
    24181 tattacagtt gtggcgactg gtcccttgac tagcgatgcc ctggctgaaa agattcatgc
    24241 ccttaataat ggcgatggct tctatttcta cgatgcagca gcgcctatta tcgatgtcaa
    24301 cactatcgat atgagtaagg tctatctcaa atctcgttat gataagggag aagcagccta
    24361 tctcaatgct ccaatgacca agcaagagtt tatggatttc catgaagcct tggtcaatgc
    24421 ggaagaagca ccgctcaatt cttttgaaaa agaaaagtac tttgaaggat gtatgccaat
    24481 cgaagttatg gccaaacgtg gcattaaaac tatgctttat ggccctatga agccagtcgg
    24541 tcttgagtat ccggacgact acacaggacc tcgtgatgga gaatttaaaa caccttatgc
    24601 ggttgtgcaa cttcgtcagg ataatgcagc tggtagccta tacaatattg ttggtttcca
    24661 gacccacctc aaatggggag aacaaaagcg tgtcttccaa atgattccgg gtcttgaaaa
    24721 tgcggagttt gtccgttatg gtgtcatgca tcgcaattct tacatggatt caccaaatct
    24781 tcttgagcag acttaccgtt ccaagaaaca accaaatctc ttctttgctg gtcagatgac
    24841 gggggtggaa ggctatgttg agtcggcggc ttcgggctta gttgcgggaa ttaacgcagc
    24901 tcgtctcttc aaggaagaaa gcgaggttat tttccccgag acgacagcga ttggaagctt
    24961 agctcattac attacccatg ccgacagcaa acatttccaa ccaatgaatg tcaattttgg
    25021 gatcatcaag gagttggaag gcgagcgtat ccgtgataag aaggctcgtt atgaaaaaat
    25081 tgcagagcgt gcccttgccg acttagagga atttttgact gtctaatttt tttgaaagaa
    25141 ttgttcatga tactataaaa atcttagaaa ttgtgataaa ataggtagga tgaaagaagg
    25201 agagtgaaaa tggcgaatcc caagtataaa cgtattttaa tcaagttatc aggtgaagcc
    25261 cttgccggtg aacgtggcgt agggattgat atccaaacag ttcaaacaat cgcaaaagag
    25321 attcaagaag ttcatagctt aggtatcgaa attgcccttg ttattggtgg aggaaatctc
    25381 tggcgtggag aacctgcagc agaagcaggt atggaccgtg ttcaggcaga ttacactgga
    25441 atgcttggga ctgttatgaa tgctcttgtg atggcagatt cattgcaaca agttggggtt
    25501 gatacgcgtg tacaaacagc tattgctatg caacaagtgg cagagcctta tgtccgtgga
    25561 cgtgcccttc gtcaccttga aaaaggccgt atcgttatct ttggtgctgg aattggttca
    25621 ccatacttct cgacagatac aacagcggcc cttcgtgcag ctgaaatcga agcagatgcc
    25681 atcctcatgg ctaaaaatgg cgtcgatggt gtgtacaatg ccgatcctaa gaaggacaag
    25741 acagccgtta agtttgaaga attgacccac cgtgatgtta tcaacaaagg tcttcgtatc
    25801 atggactcaa cagcctcaac cctctcaatg gacaacgaca ttgacttggt tgtcttcaac
    25861 atgaaccaat caggcaacat caaacgtgtt gtatttggtg aaaatatcgg aacaacagtt
    25921 tcaaataata tcgaagaaaa ggaataagaa agaatatggc taacgtaatt attgaaaaag
    25981 ctaaagagag aatgacccag tctcaccaat cacttgctcg tgaatttggt ggtatccgtg
    26041 ctggtcgtgc caatgcaagc ttgcttgacc gtgtacatgt agaatactat ggagtcgaaa
    26101 ctcctcttaa ccaaatcgct tcaattacga ttccagaagc gcgtgttttg ttggtaacac
    26161 catttgacaa gtcttcattg aaagacatcg aacgtgcctt gaacgcttct gatcttggta
    26221 tcacaccggc taatgacggt tctgtgattc gcttggttat cccagctctt acagaagaaa
    26281 ctcgtcgtga ccttgctaaa gaagtgaaga aggtcggcga aaatgctaaa gtggctgtcc
    26341 gcaatatccg tcgcgatgct atggacgaag ctaagaagca agaaaaagca aaagaaatca
    26401 ctgaagacga attgaagact cttgaaaaag atattcaaaa agtaacagac gatgctgtta
    26461 aacacatcga cgacatgact gctaacaaag agaaagaact tttggaagtc taaaaataaa
    26521 cagaaaaact cagttggcat tgctggctga gttttattcg aaagaaggaa atatgaatac
    26581 aaatcttgca agttttatcg ttggactgat catcgatgaa aatgaccgtt tttactttgt
    26641 gcaaaaggat ggtcaaacct atgctcttgc taaggaagag ggccaacata cagtagggga
    26701 tacggtcaaa ggttttgcat acacggatat gaagcaaaaa ctccgcctga caaccttaga
    26761 agtgactgcc actcaggacc aatttggttg gggacgtgtc acagaggttc gtaaggactt
    26821 gggtgtcttt gtggatacag gccttcctga caaggaaatc gttgtgtcac tcgatattct
    26881 ccctgtgctc aaggaactct ggcctaagaa gggcgaccaa ctctacatcc gtcttgaagt
    26941 ggataagaaa gaccgtatct ggggcctctt ggcttatcaa gaagacttcc aacgtctcgc
    27001 tcgtcctgcc tacaacaaca tgcagaacca aaactggcca gccattgttt accgtctcaa
    27061 gctgtcagga acttttgttt acctaccaga aaataatatg cttggtttta ttcatcctag
    27121 cgagcgttac gcagagccac gtttggggca agtattagat gcgcgcgtta ttggtttccg
    27181 tgaagtggac cgcactctga acctctccct caaaccacgc tcctttgaaa tgttggaaaa
    27241 cgatgctcag atgattttga cttatttgga aagtaatggc ggtttcatga ccttaaatga
    27301 taagtcatct ccagacgaca tcaaggcaac ctttggcatt tctaaaggtc agttcaagaa
    27361 agctttaggt ggtcttatga aggctggtaa aatcaagcag gaccagtttg ggacagagtt
    27421 gatttaggga ggcttatgag aaaatcattt tacacttggc tcatgaccga gcgcaatcct
    27481 aaaagtaaca gtcccaaagc aattttggca gacctcgctt ttgaagaggc agcttttcca
    27541 aaacatacag atgattttga tgaggtcagt cgctttttgg aggagcatgc cagtttctct
    27601 tttaacctag gagattttga cagcatttgg caggaatatc tagaacacta gcatttattc
    27661 attgggtttg ggctagtaat ttctccatcc ctctgctata ataaaaagaa ataaaaggat
    27721 tagagaggtt ctttatttga aggaacattc aatagacatt caactgagtc atccagatga
    27781 cctgtttcat ctttttggtt ccaatgaacg ccatcttcgt ttgatggaag aagagcttga
    27841 tgttgtgatt catgctcgta cggagattgt ccaggttttg ggagaagagt ctgcctgtga
    27901 ggaagcccgt caagttattc aggctttgat ggtcttggta aatcgtggga tgaccgttgg
    27961 tacgccagat gtagtcactg cgattagcat ggtcaaaaat gatgaaattg acaagtttgt
    28021 cgccctttac gaagaagaaa ttatcaaaga taatactggg aaacctatcc gtgtcaaaac
    28081 cctagggcaa aagctttatg tggacagtgt caaacagcat gatgtgacct ttggaattgg
    28141 gccagcaggt acagggaaga ccttccttgc agtgaccttg gcagtgactg cccttaaacg
    28201 tgggcaagtc aagcgaatta tcctaactcg tccagcggtg gaagcgggag agagtcttgg
    28261 atttcttccg ggtgatctta aggagaaggt ggatccttac cttcgtcctg tttacgatgc
    28321 cttgtatcag attctgggga aagaccaaac aactcgactc atggagcgtg aaattatcga
    28381 gattgcgccc cttgcctata tgcgtggacg gaccttggat gatgcctttg tcattctcga
    28441 tgaggcgcaa aatacgacca tcatgcagat gaagatgttc ttgactcgtt taggttttca
    28501 ttctaagatg attgtcaatg gagatattag tcagattgac ctgccacgta atgtcaagtc
    28561 cggtttgatt gatgctcaag agaaactcaa gaacatccat cagattgact ttgttcattt
    28621 ttcagccaag gatgtggttc gccatccagt tgtcgctcag attatccgag cctatgaata
    28681 ttctactgaa gttgcacacg actgattttg aggaagtttg cctgcaaaag aatatacttt
    28741 tgagagagga aaatccagtt gtataggcta aaggttttat ccaaaggttt gagacaacga
    28801 ttaggcacga tggaaagaac ttttatgtgg ctgatgacga tcagtgcatc ttcctgtgtc
    28861 ataatcacag ggcacaagaa agtaggaatt tgaaaagatg attgaccaac tatctaagta
    28921 ttacagttgt aggatactaa ctgaaaagga tattccaagt attttatctt tatatgaaag
    28981 taatcctctg tattttcagc attgtccacc agagccaaat tttgcaactg taaaagagga
    29041 catgctttgt ctacctgaag gtaaagctaa ggctgataag ttttttgttg gattttggaa
    29101 tggatctgac cttgtggctg ttatagattt tgtctatgca catcctgatg aggagactgt
    29161 ttttattggt ttgtttatgg ttgatcaagc ctatcagagg aaagggattg gtagtcatat
    29221 tgtgacagaa gcactagctt attttgctaa gaactttcga aaggcacgtt tggcttatgt
    29281 taagggaaat ccgcaatctc agcatttttg ggaaaagcag ggctttaaat caattggatg
    29341 cgaggttaag caagaactct atacggttgt tatcgctgaa cagagcctag aagattagaa
    29401 atggcatcaa gtaagaacta tttggaattt gttttggaac aattatcagg attagatgat
    29461 gtgacttacc gttccatgat tggggagtat attctttact tccgcggcaa gattattggc
    29521 ggcatttatg acgatcgctt tttagttaaa cccgtgcaag cagtcttaga taagattgac
    29581 caatcttctt ttgagtttcc atacaaaggt gccaaagaaa tgatttgagt ggaagaactt
    29641 gataataaga tgtttctata agacctgatt ttagctatgt ataaccaact gccaacgccc
    29701 aaacctaaaa agaaaaagca agggtgaacg aagtaaaaaa gaagtctgct aaggccctgt
    29761 ctttgcacgg gtaaaatttt atatataaaa agaagctggg actaaagagc tcagcttcct
    29821 ttggtttata taattgtcat tacaagacga agtggttggg cgaaactctg ttgactttat
    29881 tcaatttaga gtttcttatg cacaattgag tctggaacga aagtctccag ttgcaaagta
    29941 tacagtacaa taaaccaacg atgtaatagc tgatgacaca aagcacagtg ggtaggactt
    30001 gcgaagtcac ccttttcttt tcaaaattta tactaaatca ttgatatcag tgtagtcacg
    30061 attaagtcct tgagcaactg gtaggctagt caagtaacct tgataagtgg tcacaccttg
    30121 acgcaagcct tcatcttcag agattgcttg tgcgaatcct ttgccagcca aagcttcgat
    30181 ataaggaaga gtgacattgg ttagggcgat ggttgaagtg cgggcaaccg caccagggat
    30241 attggcaacg gcatagtgga gaacaccgtg tttttcatag acgggttcat cgtgcgttgt
    30301 cacacggtca gctgtttcga taacgccacc ttggtcaaca gcaacgtcaa cgatacagag
    30361 cctggacgca tttgtttgac catctcatct gtcaccaatt ccggtgcttt tgcaccaggg
    30421 atgagaatgg ctccaatcac cacatcagca tctctcatac ttgcttcaat gttgaatgaa
    30481 ttagatataa gagtttgaat ttgacttcca aagacttctt ctagaactga gagacgcttg
    30541 gcactaatat ctaaaatagt cacttgagca ccaagaccaa gggcgatgcg ggcagcatgt
    30601 gtaccgacga caccatcacc gatgatagtt acttttcctt ttggaacacc tggtacacca
    30661 ccaggtagaa caccagagcc accagcttgc ttagtaagga agtgagctcc gatttaaaca
    30721 gccatacgac ctgcaacctc actcatagga acgaggagcg gtagttgtcc ttgattgtca
    30781 cgaacagttt cataggcaat tcctgttgtt tttgctgcta acatagcatc tgctaattct
    30841 ggagcagcgg ccatgtgcaa gtaggtgaag agaagaagat cgtcgcgcaa gtaaccgtat
    30901 tcagaactta aaggttcttt tactttcaca accaactctg ctgcccaagc ttcaccagca
    30961 gtagcgacaa tctcagctcc ttgcttttga tagtcagcat cagtaaagcc agaaccgaga
    31021 ccagcatttg tttcgataag gacacgatga ccacgactaa ctaagctatg aacacctgca
    31081 ggtgtgaggg cgacacggtt ttcgttattt ttaatttctt ttgggattcc gattaacatt
    31141 gagataacct acctttcaat tgacggtctt gttttggttg tcacattcca gttcataaat
    31201 caaaaatgtg acggtttcat tgtatatgaa accgcttcaa aaatcaagaa aaacttgtca
    31261 tccaaatttt tttatgctag actagtgaaa atcaagctct aatggaggga aaagtatgga
    31321 atcaatattt gtgaaatttg cccagtatcc gtctatagaa acggagcgtt tattgcttag
    31381 acctgtaact ttggatgatg cggaagcaat gtttgactat gcctcggaca agggtaatac
    31441 acgttacact tttccaacca atcaaagctt ggaagaaacc aagaataaca ttgctcagtt
    31501 ctacttggct aatcccttgg gacgttgggg gattgaatta aaagataacg gcaagtttat
    31561 cggaactatt gacttacaca agattgatcc tgttcttaag aaggcagcta ttggctacat
    31621 tatcaataaa aagtattgga atcaaggatt aacgccagaa accaatcgtg ctgtgattga
    31681 gctagctttt gagaagatag gaatgaataa gttagttgct ttatacgata aggacaattc
    31741 tgcgtcagga aaggtcatgg agaaatcagg catgcgtttt tcccatgcag aaccatatgc
    31801 ttgtatggac cagcatgaaa aaggccgaat cgtgacaaga gttcattatg tcttgaccaa
    31861 ggaagactat tttgcaaata aataagcagt tgaaaagaaa tttttcgact gttttttctt
    31921 cctcttacga ataatctaag agaggagaaa atatggaagc aattatcgag aaaatcaaag
    31981 agtataaaat catcgtcatc tgtactggtc tgggcttgct tgtaggcgga tttttcctgc
    32041 taaagccagc tccacaaaca cctgtaaagg aaacgaattt gcaggctgaa gttgcagctg
    32101 tttccaagga tttggtatcc gaaaaggaag tgaacaagga agagaaggaa gaaccccttg
    32161 aacaagatct aatcacagta gatgtcaaag gtgctgtcaa atcgccaggg atttatgact
    32221 tgcctgtagg tagtcgagtc aatgatgctg ttcagaaggc tggtggcttg acagagcaag
    32281 cagacagcaa gtcgctcaat ctagctcaga aagttagtga tgaggctctg gtttacgttc
    32341 ctactaaggg agaagaagca gttagtcaac agactggttc ggggacagct tcttcaataa
    32401 gcaaggaaaa gaaggtcaat ctcaacaagg ccagtctgga agaactcaag caggtcaagg
    32461 gactgggagg aaaacgagct caggacatta tcgaccatcg tgaggcaaat ggcaagttca
    32521 agtcagtaga cgagctcaag aaggtctctg gcattggtgg caaaacaata gaaaagctta
    32581 aagactatgt tacagtggat taagaatttc tctattcccc taatttacct gagttttcta
    32641 ttactttggc tttattacgc tattttctca gcatcttatc ttgctttgtt gggctttgtt
    32701 tttctgctag tctgtctctt tatccaattt ccgtggaaat ctgctggtaa agttctaata
    32761 atttgcggaa tctttggatt ttggtttgtt tttcaaaatt ggcaacagag tcaagcgagt
    32821 caaaatctgg cggattctgt tgaaagggta cggattttgc ctgatactat taaggttaat
    32881 ggtgatagtc tatcctttcg tggcaagtct aacggtcgtg ctttccaagt ctattataaa
    32941 ctccagtccg aggaggagaa agaagccttt caagctttaa ccgacctgca tgagatagga
    33001 ctagaaggga agctttcgga gccagaaggg cagagaaatt ttggtggctt taattaccaa
    33061 gcctatctga agactcaggg aatttaccag actctcaata tcaaaaaaat ccagtcactt
    33121 caaaagattg gcagttggga tataggagaa aacttgtcca gtttacgtcg aaaggctgtg
    33181 gtttggatta agacgcactt tccagaccct atgcgcaatt acatgacagg actcttgctg
    33241 ggacatctgg acaccgactt tgaggagatg aatgagcttt attccagtct aggaattatc
    33301 cacctctttg ccctatctgg tatgcaggta ggttttttca tgaatggatt taagaaactt
    33361 ctcttgcgat tgggcttgac ccaagaaaag ttgaaatggc tgacttatcc cttttccctt
    33421 atctatgcgg gactaactgg attttcagca tcggttattc gcagtctctt gcaaaagcta
    33481 ctggctcaac atggggttaa gggcttggat aattttgcct tgacggtgct tgtcctcttt
    33541 attgtcatgc caaacttttt cttgacagca ggaggagtct tgtcctgcgc ttatgctttt
    33601 atcctgacca tgaccagcaa agaaggggag gggctcaagg ctgttactag tgaaagtcta
    33661 gtcatctcct tgggcatatt gcccattcta tccttctatt ttgcggaatt tcaacctttg
    33721 tctatccttt tgacctttgt cttttccttt ctatttgact tgaccttctt accgctcttg
    33781 tctattttat ttgtcctttc ctttctctat ccagtcattc agctgaactt tatctttgaa
    33841 tggttggagg gcattattcg cttggtgtca caggtgacaa gtagacctct ggtctttgga
    33901 caacccaatg catggttttt aatcctattg ttaatttcct tggctttggt ctatgatttg
    33961 agaaaaaaca ttaaaaagct aacggtattg tgcttattga ttacagggct ctttctcctg
    34021 accaagcatc cactggaaaa tgaaatcacc atgctggatg tggggcaagg agaaagtatt
    34081 ttcctacggg atgtaactgg gaaaaccatt ctcatagatg taggtggtaa ggcagaatct
    34141 tataagaaaa tcgaaaaatg gcaagaaaaa atgacgacca gcaatgccca gcgaaccttg
    34201 attccctatc tcaaaagtcg aggagtagct aagattgacc agctaatttt gactaacacg
    34261 gacaaggaac atgtcggaga tttgttagag gtgaccaagg ctttccatgt aggggagatt
    34321 ttagtatcaa aaggcagttt gaagcagaag caatttgtgg tagaactaca ggcgactcaa
    34381 acaaaggtgc gtagtatgac agtaggggag aacttgccca tttttggaag tcagttagaa
    34441 gttctatccc caaggaaaat gggagatgga ggacacgatg ataccctagt tctgtatggg
    34501 aaattcttgg ataagcaatt tctcttcacg ggaaatttgg aggagaaagg agagaaggac
    34561 ttgctgaagc actatccaga cttgaaagta aatgttttga aagctagcca acatggcaat
    34621 aaaaaatcat caagtccagc ctttctagaa aaactcaaac cagagcttac tcttatctca
    34681 gttggaaaga gcaatcgaat gaaactcccc catcaggaaa cattgacacg actggaaggt
    34741 atcaatagca aagtttaccg aactgaccag caaggagcta tacggtttaa aggttggaat
    34801 agttggaaaa tcgaaagtgt tcgataggaa agataaatgt tgtagattag tgaaataaac
    34861 taaaaatttg ttgcataata atgataaaaa tggtataatg aaaacgtatt caatactgag
    34921 gatataaaat cattaaaaat tagcaaaaat cgtttaatta gttagtttat gattattggt
    34981 ctttttcagt ccagtatatc tgctgtgaca gtcactaaaa gttacaagta tgattggaat
    35041 acggtttggg aatatagtac taactatcac gaccatcagt atgcttggat tccgtcatgg
    35101 tctcgttatt acagctattc tgagtatcca gttggttctg gctggaacta cgctcgttat
    35161 gaggtcataa actactatac tggaggttat taatccttaa agagtgagaa aaggagggct
    35221 ggatatgtta aggcttactc atgttaccct aaaaacgcga caagtcatct tgcaagatgc
    35281 ggattttacc tttaaaaagg gtagggttta tggtcttctt gctatcaatg gctcgggaaa
    35341 gacgacacta ttccgagcta tgagcaagtt gcttcccctt agtagtggac acatcgcagt
    35401 tcctccttct ttgttttatt atgagagcgt tgaatggctg gatggaaact taagtgggat
    35461 ggactacctt cgtctcataa aaaacatctg gaagtcagac ctaaacttga gagatgaaat
    35521 cgcctactgg gaaatggctg actatatcag tcttcccatc cgcaagtatt ccttaggcat
    35581 gaagcaacgc ttggtgattg ctatgtattt tctcagtcag gccaaatgct ggctcatgga
    35641 tgagattaca aatggcttag acgagtatta tcgacagaag ttttttgata ggctagcaca
    35701 aatcgataga caagaacagc tggttctttt aagttcccac tataaggaag agttggttga
    35761 tatctgcgat agagtagtaa ccattcatca ggggcagata gaagaggttt agtttatgaa
    35821 agatgttagt ctatttttat tgaaaaaagt tttcaaaagt cgtttaaact ggattatctt
    35881 acttttattt gcatctgtac tcggtgttac cttttattta aatagtcaga ctgcaaactc
    35941 acacagcttg gagagcaggt tggaaagtcg cattgcagcc aacgagaggg ctatcaatga
    36001 aaatgaagag aaactctccc aaatgtctga taccagctcg gaggaatacc agtttgctaa
    36061 aaataattta gacgtgcaaa aaaatctttt gacgcgaaag acagaaattc taactttact
    36121 aaaagaaggg cgctggaaag aagcctacta tctgcagtgg caagatgaag agaagaatta
    36181 tgaatttgta tcaaatgacc cgactgctag ctctggctta aaaatggggg ttgaccgcga
    36241 acggaagatt taccaagccc tgtatccctt gaacataaaa gcacatactt tggagtttcc
    36301 gacccacggg attgatcaga ttgtctggat tttagaggtt atcatcccaa gcttgtttgt
    36361 ggttgcgatt atttttatgc taacacaact atttgcagaa agatatcaaa atcatctgga
    36421 cacagctcac ttatatcctg tttcaaaagt gacatttgca atatcctctc ttggagttgt
    36481 agtgggatat gtaactgtgc tgtttatcgg aatctgtggc ttttcttttc tagtgggaag
    36541 tctgataagt ggttttggac agttagatta tccctaccca atttatagct tagtgaatca
    36601 agaagtaact attgggaaaa tacaagatgt attatttcct ggcttgctct tagctttctt
    36661 agcctttatc gtcattgtgg aagtcgtcta cttgattgct tactttttca agcaaaaaat
    36721 gcctgtcctc tttctttcac tcattgggat tgttggctta ttgtttggca tccaaacaat
    36781 tcagcctctt caaaggattg cacatctgat tccctttact tacttgcgtt cagtggagat
    36841 tttatctgga agattatcta agcagattga taatgtcgat ctaaattgga gcatggggat
    36901 ggtcttactt ccttgcctga ttatcctttt gttagtgggg attctattta tcgaaagctg
    36961 gggaagttca cgtaaaaagg aagtttttaa agtttagttt tcctataggg aagctaaaga
    37021 aaaactaaca tagggagaga atcaacttgg ttctctcttt ttgattcgaa aaccaaacca
    37081 aaatacaaac acaaactttt caaaaaataa ctttttatct tgacaagagc tagaaaactt
    37141 ggtatcatat aaaagttgag aaaagcagaa gtgagagctt ctcgccttgt gacattaagt
    37201 tgcctggccc tacggatgaa aagtttcgaa gaaacgctat cataacgtgc gggcttgtat
    37261 atttacaagt ccgctattgt ttttctctaa taaaacaaaa gaggtgaaaa ccatagcaaa
    37321 gcaagactta ttcatcaatg atgagattcg tgtacgtgaa gttcgcttga ttggtcttga
    37381 aggagaacag ctaggtatca agccactcag tgaagcgcaa gctttggctg ataacgctaa
    37441 tgttgaccta gtattgattc aaccccaagc caaaccgcct gttgcaaaaa ttatggacta
    37501 cggtaagttc aaatttgagt accagaagaa gcaaaaagaa caacgtaaaa aacaaagcgt
    37561 tgttactgtg aaagaagttc gtctaagtcc aactattgac aagggtgact ttgatacaaa
    37621 acttcgtaat gcacgtaagt tccttgaaaa aggaaataaa gttaaggtat ctattcgctt
    37681 taagggtcgt atgattaccc ataaagagat tggtgcaaaa gttttagccg agtttgctga
    37741 agcaactcaa gatattgcca tcatcgaaca acgtgctaaa atggatggac gtcaaatgtt
    37801 catgcaattg gcgccagcga ctgacaaaaa ataattgtca gaaagttaaa taaaggagaa
    37861 aaaatcatgc caaaacaaaa aacacaccgc gcatcagcta aacgtttcaa acgtacaggt
    37921 tctggtggac ttaaacgttt ccgtgcttac acttctcacc gtttccacgg aaaaactaag
    37981 aaacaacgtc gtcatcttcg taaagcatct atggtgcatt caggagatta caaacgtatc
    38041 aaagcaatgc ttactcgctt gaaataatag cttgactgta agtaaacaat tctaggaaat
    38101 atttggagga aataaaacat ggcacgtgtt aaaggtggcg ttgtatcacg caaacgtcgt
    38161 aaacgtattc ttaaattagc aaaaggttac tatggagcta aacacatctt gttccgtact
    38221 gcaaaagaac aagtaatgaa ctcttactac tatgcatacc gtgaccgtcg tcagaaaaaa
    38281 cgtgacttcc gcaaattgtg gattactcgt atcaatgcgg cagctcgtat gaacggactt
    38341 tcatactcac aattgatgca tggtttgaaa ttggctgaga ttgaagttaa ccgtaaaatg
    38401 cttgctgact tggctgttaa cgatgcagta gctttcacag ctcttgcaga tgcagctaaa
    38461 gcaaaacttg gtaaataaca actgatgaga ctggaacagg attgtccagt ctttttaaaa
    38521 ataaaggaga aagatatggc ttcaaaaatg ctacacactt gcttacgagt agaaaatctt
    38581 gaaaaatcaa tcgcatttta tcaagatgct tttggtttta aagaattgcg tcgcagagat
    38641 tttccagacc atgccttcac gattgtctat ctaggtcttg agggtgatga ttatgagttg
    38701 gagttgactt ataactacga tcacggtccg tatgtggttg gtgatgggtt tgcccatatc
    38761 gccctcagta cacctgatct tgaggcactt catcaagaac acagtgcaaa aggctatgaa
    38821 gtgactgagc caaatggtct accaggaact acaccaaact attactttgt taaagaccct
    38881 gatggatata aggtcgaagt cattcgtgaa aaataatttc gtgcaagtta agtagaatat
    38941 tcgttttcta cttgactttt tttagctgaa agagtatact aataaaaatt caacctttaa
    39001 ggggagtcca gagagactca caaggtgtca gataaaagaa tagtgcaatt ttctagagga
    39061 gaccttttga gtgtgctctc ttgtgttgta cgattttaac tgaggccttg cactagcaag
    39121 gtcttttctt tatctggtcc ccttaaaatt taaggaggaa aagttatgaa tctcacatgt
    39181 aagaagcgtt tgggtgtcat tcggttggaa accatgaagg tggttgcaca agaggaaatc
    39241 gcgccagcaa tctttgaatt agtcctagaa ggagaaatgg ttgaagccat gcgagcaggc
    39301 caatttcttc atctgcgtgt accagacgat gcccatctct tacgtcgtcc tatttcaatt
    39361 tcgtctattg acaaggcaaa caagcagtgt cacctcattt atcggattga gggagctggg
    39421 actgcaattt tttcaacctt aagtcaggga gacactcttg atgtgatggg gcctcaggga
    39481 aatggttttg acttgtctga ccttgatgag cagaatcagg ttctccttgt tggtggtggg
    39541 attggtgttc cacccttgct tgaggtggcc aaggaattgc atgaacgtgg agtgaaagta
    39601 gtgacagtcc tcggttttgc taataaggat gctgttattt tgaaaacgga attggctcag
    39661 tatggtcagg tctttgtaac gacagatgat ggttcttatg gcatcaaggg aaatgtttcc
    39721 gttgttatca atgatttaga tagtcagttt gatgctgttt actcgtgtgg ggctccagga
    39781 atgatgaagt atatcaatca aacctttgat gatcacccaa gagcctattt atctctggaa
    39841 tctcgtatgg cttgtgggat gggagcttgc tatgcctgtg ttctaaaagt accagaaagc
    39901 gagacggtca gccaacgcgt ctgtgaagat ggtcctgttt tccgcacagg aacagttgta
    39961 ttataaggag aaaattatga ctacaaatcg attacaagtg tctctacctg gtttggattt
    40021 gaaaaatccg attattccag catcaggctg ttttggcttt ggacaagagt atgccaagta
    40081 ctatgattta gaccttttag gttctattat gatcaaggcg acaacccttg aaccacgttt
    40141 tgggaatcca actccaagag tggcagagac gcctgctggt atgctcaatg caattggctt
    40201 gcaaaatcct ggtttagagg ttgttttggc tgaaaagcta ccttggctgg aaagagaata
    40261 tccaaatctt cctattattg ccaatgtagc tggtttttca aaacaagagt atgcagctgt
    40321 ttctcatggg atttccaagg caactaatgt aaaagctatc gagctcaata tttcttgtcc
    40381 caatgttgac cactgtaatc atggactttt gattggtcaa gatccagatt tggcttatga
    40441 tgtggtgaaa gcagctgtgg aagcctcaga agtgccagtt tatgtcaaat taaccccgag
    40501 tgtgaccgat atcgttactg tcgcaaaagc tgcagaagat gcgggagcaa gtggcttgac
    40561 tatgatcaat actctggttg gaatgcgctt tgacctcaaa accagaaaac caatcttggc
    40621 caatggaaca ggtggaatgt caggtccagc agtctttcca gtagccctca aactcatccg
    40681 ccaagtagcc caaacaacag acctgcctat cattggaatg gggggagtgg attcggctga
    40741 agctgcccta gaaatgtatc tggctggggc atctgctatc ggagttggaa cagctaactt
    40801 taccaatcct tatgcctgcc ctgacatcat cgaaaattta ccaaaagtca tggataaata
    40861 cggtattagc agtctggaag aactccgtca ggaagtaaaa gagtctctga ggtaaactgc
    40921 aatcaatctg ttcttgattt tttattagtt tgtaatatga atttaggaga attttggtac
    40981 aataaaataa ataagaacag aggaagaagg ttaatgaaga aagtaagatt tattttttta
    41041 gctctgctat ttttcttagc tagtccagag ggtgcaatgg ctagtgatgg tacttggcaa
    41101 ggaaaacagt atctgaaaga agatggcagt caagcagcga atgagtgggt ttttgatact
    41161 cattatcaat cttggttcta tataaaagca gatgctaact atgctgaaaa tgaatggcta
    41221 aagcaaggtg acgactattt ttacctcaaa tctggtggct atatggccaa atcagaatgg
    41281 gtagaagaca agggagcctt ttattatctt gaccaagatg gaaagatgaa acgaaatgct
    41341 tgggtaggaa cttcctatgt tggtgcaaca ggtgccaaag taatagaaga ctgggtctat
    41401 gattctcaat acgatgcttg gttttatatc aaagcagatg gacagcacgc agagaaagaa
    41461 tggctccaaa ttaaagggaa ggactattat ttcaaatccg gtggttatct actgacaagt
    41521 cagtggatta atcaagctta tgtgaatgct agtggtgcca aagtacagca aggttggctt
    41581 tttgacaaac aataccaatc ttggttttac ataaaagaaa atggaaacta tgctgataaa
    41641 gaatggattt tcgagaatgg tcactattat tatctaaaat ccggtggcta catggcagcc
    41701 aatgaatgga tttgggataa ggaatcttgg ttttacctca agtttgatgg caaaatggct
    41761 gaaaaagaat gggtctacga ttctcatagt caagcctggt actacttcaa atccggtggt
    41821 tacatgacag ccaatgaatg gatttgggat aaggaatctt ggttttatct caaatctgat
    41881 gggaaaatag ctgaaaaaga atgggtctac gattctcata gtcaagcttg gtactacttc
    41941 aaatccggtg gttacatgac agccaatgaa tggatttggg ataaggaatc ttggttttac
    42001 ctcaagtctg atggcaaaat ggctgaaaaa gaatgggtct acgattctca tagtcaagcc
    42061 tggtactact tcaaatctgg tggctacatg gcgaaaaatg agacagtaga tggttatcag
    42121 cttggaagcg atggtaaatg gcttggagga aaagctacaa ataaaaatgc tgcttactat
    42181 caagtagtgc ctgtcacagc caatgtttat gattcagatg gtgaaaagct ttcctatata
    42241 tcgcaaggta gtgtcgtatg gctagataag gatagaaaaa gtgatgacaa gcgcttggct
    42301 attactattt ctggtttgtc aggctatatg aaaacagaag atttacaagc gctagatgct
    42361 agtaaggact ttatccctta ttatgagagt gatggccacc gtttttatca ctatgtggct
    42421 cagaatgcta gtatcccagt agcttctcat ctttctgata tggaagtagg caagaaatat
    42481 tattcggcag atggcctgca ttttgatggt tttaagcttg agaatccctt ccttttcaaa
    42541 gatttaacag aggctacaaa ctacagtgct gaagaattgg ataaggtatt tagtttgcta
    42601 aacattaata atagcctttt ggagaacaag ggcgctactt ttaaggaagc cgaagaacat
    42661 tatcatatca atgcccttta tctccttgcc catagtgccc tagaaagtaa ctggggaaga
    42721 agtaaaattg ccaaagataa gaataatttc tttggcatta cagcctatga tacgacccct
    42781 tacctttctg ccaagacatt tgatgatgtg gataagggaa ttttaggtgc aaccaagtgg
    42841 attaaggaaa attatatcga taggggaaga actttccttg gaaacaaggc ttctggtatg
    42901 aatgtggaat atgcttcaga cccttattgg ggcgaaaaaa ttgctagtgt gatgatgaaa
    42961 atcaatgaga agctaggtgg caaagattag tactataagt gaatatgatt tgagtgaata
    43021 gtaagttaaa aatcctgatt tcaagtaaaa tcaggatttt ttcatggatg caattttttt
    43081 ggagtctggt gtgacgcgga gggtcttttg tcctgtgtaa gtgacaaagc cgggttttcc
    43141 accagttggt ttattgagtt ttttgacttc aatcatatct acctgcacca gattcgacag
    43201 gcgcccttga gagaagtagg cagctaactc tgctgcgtct gtcttgactg catcagatgg
    43261 gtcaagattt cctgagatga caacatggct tccaggaatg tccttagcat ggaaccaaag
    43321 ttcctccttg cgggccattt taaaggtcaa ttcctcattt tgaagattgt ttcgtccgac
    43381 atagatgatg gttttgccat cgcttgctag atattgttct agttttttgc gtttctggat
    43441 tttctcccgt tgtcttctgc ggataaaacc tgtttgaatc aattcttcac ggatttcagc
    43501 gatttcttcc agtccagctt ggttgaggac ggtttctaca ctttccagat agagaatagt
    43561 ggctttggtt tcttcaatca aatcagtcaa gtatttgaca gcttctttga gtttctgata
    43621 ccgtttaaaa tagcgttggg cattctggtt gggagtcaga gccttatcaa gcgcaatcat
    43681 gataggttgg ttggtatagt agttgtctag gataacctgg tcttggtcgt taggcacttg
    43741 gtgtaggaag gttgtcagca attctccttt ttgacgaaat tcttcagcgt tgtctgtcgc
    43801 cagtaactct ttttcctgtt ttttgagttt gtgtcggttt ttctgaagtt cattttcaac
    43861 acgacgaatc agttcactgg cctgctgttt gacgcggtcg cgctcagcct tatccttata
    43921 gtaggtgtcc aacaaatcag aaagatttgc aaaaggctct cccacctgat ttgcaaaagg
    43981 aactggactg aaggaagtct cagtcaagca tggcttggtt tcttgattga aaaaatttcg
    44041 gaaagcggaa agtttttcac taaccagtat cctttccaat tcatttgcag tatcgcgtcc
    44101 cagaccttga aagaggcttt gaagattttt tgctgttagt tcttgggttt gcaggatttc
    44161 aaagagcttt tcatctttga tagtaaaagg attgagagat tctgtacttg gcggagcgat
    44221 ataggtcgat cctggaagta aggtgcggta gctattttgt gaaaaaccga cgtgtttgat
    44281 aacttcgagg attttatgac tgcttttatc gaccagtaga atattactgt gtttccccat
    44341 aatttcaata atcaaggtag cctggatatg gtctccaatc tcgtttttat tggaaactgt
    44401 aatttccaca atacggtcat tttccacttg ctcaatcgac tcaatcaggg ccccctgcaa
    44461 atactttctc aaaaccatga taaaggtaga aggttgagct ggattttcaa aagtcgtttg
    44521 ggtcagctga atgcgtccaa aaactggatg ggcagaaagg agcaggcgat ggctttggcg
    44581 attgctgcgg atttgcaaga ccaactcttg ttcaaaaggc tgattgattt tctggatgcg
    44641 accattcact aattcgcttc gcaattcctc aactatgtgg tgtaaaaaaa atccgtcaaa
    44701 tgacatcgtt ctctccttgt gattgtattc catagtatta tatcaaaaag gtagaataaa
    44761 atcatggaaa tgtggtataa taaagccaag taaagagaaa cgagaagcac atgtatattg
    44821 aaatggtaga tgaaactggt caagtttcaa aagaaatgtt gcaacaaacc caagaaattt
    44881 tggaatttgc agcccaaaaa ttaggaaaag aagacaagga gatggcagtc acttttgtga
    44941 ccaatgagcg tagtcatgaa cttaatctgg agtaccgtga caccgaccgt ccgacagatg
    45001 tcatcagcct tgagtataaa ccagaattgg aaattgcctt tgacgaagag gatttgcttg
    45061 aaaatccaga attggcagag atgatgtctg agtttgatgc ctatattggg gaattgttca
    45121 tctctatcga taaggctcat gagcaggccg aagaatatgg tcacagcttt gagcgtgaga
    45181 tgggcttctt ggcagtacac ggctttttac atattaacgg ctatgatcac tatactccgg
    45241 aagaagaagc ggagatgttc ggtttacaag aagaaatttt gacagcctat ggactcacaa
    45301 gacaataaac gaaaatggaa aaatcgtgac ttgatatcca gtttagaatt tgccatcaca
    45361 gggattttta ctgctatcaa ggaagaacga aatatgcgaa aacatgcagt gacggctcta
    45421 gtggtcatcc ttgcaggttt tgtttttcag gtgtcacgaa tcgaatggct ctttcttcta
    45481 ttgagtattt tcttggtagt agcctttgag attatcaact ctgctattga aaatgtggtg
    45541 gatttggcca gtcactatca cttttccatg ctggctaaaa atgccaagga tatggcggcc
    45601 ggcgcggtat tagtggtttc tctttttgca gccttaacag gcgcattgat ttttctccca
    45661 cgaatctggg atttattatt ttaaacagta agaggaaatt atgactttta aatcaggctt
    45721 tgtagccatt ttaggacgtc ccaatgttgg gaagtcaacc tttttaaatc acgttatggg
    45781 gcaaaagatt gccatcatga gtgacaaggc gcagacaacg cgcaataaaa tcatgggaat
    45841 ttacacgact gataaggagc aaattgtctt tatcgacaca ccagggattc acaaacctaa
    45901 aacagctctc ggagatttca tggttgagtc tgcctacagt acccttcgcg aagtggatac
    45961 cgttcttttc atggtgcctg ctgatgaagc gcgtggtaag ggggacgata tgattatcga
    46021 gcgtctcaag gctgccaagg ttcctgtgat tttggtggtg aataaaatcg ataaggtcca
    46081 tccagaccag ctcttgtctc agattgatga cttccgtaat caaatggact ttaaggaaat
    46141 tgttccaatc tcagcccttc agggaaataa cgtgtctcgt ctagtggata ttttgagtga
    46201 aaatctggat gaaggtttcc aatatttccc gtctgatcaa atcacagatc atccagaacg
    46261 tttcttagtt tcagaaatgg ttcgcgagaa agtcttgcac ctaactcgtg aagagattcc
    46321 gcattctgta gcagtagttg ttgactctat gaaacgagac gaagagacag acaaggttca
    46381 catccgtgca accatcatgg tcgagcgcga tagccaaaaa gggattatca tcggtaaagg
    46441 tggcgctatg cttaagaaaa tcggtagtat ggcccgtcgt gatatcgaac tcatgctagg
    46501 agacaaggtc ttcctagaaa cctgggtcaa ggtcaagaaa aactggcgcg ataaaaagct
    46561 agatttggct gactttggct ataatgaaag agaatactaa gtagaggtag gctcatgcct
    46621 gcttcttgtt tttacagaag gaggacttat gcctgaatta cctgaggttg aaaccgtttg
    46681 tcgtggctta gaaaaattga ttataggaaa gaagatttcg agtatagaaa ttcgctaccc
    46741 caagatgatt aagacggatt tggaagagtt tcaaagggaa ttgcctagtc agattatcga
    46801 gtcaatggga cgtcgtggaa aatatttgct tttttatctg acagacaagg tcttgatttc
    46861 ccatttgcgg atggagggca agtattttta ctatccagac caaggacctg aacgcaagca
    46921 tgcccatgtt ttctttcatt ttgaagatgg tggcacgctt gtttatgagg atgttcgcaa
    46981 gtttggaacc atggaactct tggtgcctga ccttttagac gcctacttta tttctaaaaa
    47041 attaggtcct gaaccaagcg aacaagactt tgatttacag gtctttcaat ctgcccttgc
    47101 caagtccaaa aagcctatca aatcccatct cctagaccag accttggtag ctggacttgg
    47161 caatatttat gtggatgagg ttctctggcg agctcaggtt catccagcta gatcttccca
    47221 gactttgaca gcagaagaag cgacagctat tcatgaccaa accattgctg ttttgagcca
    47281 ggctgttgaa aaaggcggtt ccaccattcg gacttatacc aatgcctttg gggaagatgg
    47341 aagcatgcag gactttcatc aggtctatga taagactggt caagagtgtg tacgctgtgg
    47401 taccatcatt gagaaaattc aactaggcgg acgaggcacc cacttttgtc caaactgtca
    47461 aaggagggac tgatgagaaa aatcatcgga atcactgggg gaattgcctc tggtaagtca
    47521 actgtgacaa attttctaag acagcaaggc tttcaagtag tggatgccga cgcagtcgtc
    47581 caccaactac agaaacctgg tggtcgtctg tttgaggctc tagtacagca ctttgagcaa
    47641 gaaatcattc ttgaaaacgg agaactcaat cgccctctcc tagctagtct catcttttca
    47701 aatcctgaag agcaaaaatg gtcgaagcaa attcaagggg agattatccg tgaggaactg
    47761 gctactttga gagaacagtt ggctcagaca gaagagattt tcttcatgga tattccccta
    47821 ctttttgagc aggactacag cgattggttt gctgagactt ggttggtcta tgtggaccga
    47881 gatgcccaag tagaacgctt aatgaaaagg gaccagttgt ccaaagatga agctgagtct
    47941 cgtctggcag cccagtggcc tttagaaaaa aagaaagatt tggccagcca ggttcttgat
    48001 aataatggca atcagaacca gcttcttaat caagtgcata tccttcttga gggaggtagg
    48061 caagatgaca gagattaact ggaaggataa tctgcgtatt gcctggtttg gtaattttct
    48121 gacaggagcc agtatttctt tggttgtacc ttttatgccc atcttcgtgg aaaatctagg
    48181 tgtagggagt cagcaagtcg ctttttatgc aggcttagca atttctgtct ctgctatttc
    48241 cgcggcgctc ttttctccta tttggggtat tcttgctgac aaatacggcc gaaaacccat
    48301 gatgattcgg gcaggtcttg ctatgactat cactatggga ggcttggcct ttgtcccaaa
    48361 tatctattgg ttaatctttc ttcgtttact aaacggtgta tttgcaggtt ttgttcctaa
    48421 tgcaacggca ctgatagcca gtcaggttcc aaaggagaaa tcaggctctg ccttaggtac
    48481 tttgtctaca ggcgtagttg caggtactct aactggtccc tttattggtg gctttatcgc
    48541 agaattattt ggcattcgta cagttttctt actggttggt agttttctat ttttagctgc
    48601 tattttgact atttgcttta tcaaggaaga ttttcaacca gtagccaagg aaaaggctat
    48661 tccaacaaag gaattattta cctcggttaa atatccctat cttttgctca atctcttttt
    48721 aaccagtttt gtcatacaat tttcagccca atcgatcggc cctattttgg ctctttatgt
    48781 acgcgactta gggcagacag agaatcttct ttttgtctct ggtttgattg tgtccagtat
    48841 gggcttttcc agcatgatga gtgcaggagt catgggcaag ctaggtgaca aggtgggcaa
    48901 tcatcgtctc ttggttgtcg cccagtttta ttcagtcatc atctatctcc tctgtgccaa
    48961 tgcctctagc ccccttcaac taggactcta tcgtttcctc tttggattgg gaaccggtgc
    49021 cttgattccc ggggttaatg ccctactcag caaaatgact cccaaagccg gcatttcgag
    49081 ggtctttgcc ttcaatcagg tattctttta tctgggaggt gttgttggtc ccatggcagg
    49141 ttctgcagta gcaggtcaat ttggctacca tgctgtcttt tatgcgacga gcctttgtgt
    49201 tgcctttagt tgtctcttta acctgattca atttcgaaca ttattaaaag taaaggaaat
    49261 ctagtgcgag taaaaatcaa tctcaaatgc tcctcttgtg gcagtatcaa ttacctaacc
    49321 agtaaaaatt caaaaaccca tccagacaag attgaggttt taaagtattg tcccaaggaa
    49381 aaaaaagtaa ccctacatct tgaatctaag tagattttat ggtaaaataa tagggatttt
    49441 aaggagtttg atatgtataa cctattatta accattttat tagtattatc tgttgtgatt
    49501 gtgattgcaa ttttcatgca accaaccaaa aaccaatcca gcaatgtatt tgatgccagt
    49561 tcaggtgatt tgtttgaacg cagtaaagct cgcggttttg aagctgtaat gcagcgtttg
    49621 acagggattt tagtcttttt ctggctagcc attgccttag cattgacggt attatcaagt
    49681 agataagaaa ataatgggca ggactaggtc tttgcctctt tttattttta aaggatgttt
    49741 gagaaggttt tacagtaaaa gaaaattaaa aaatctagaa agaaaatatg aaagatagaa
    49801 taaaagaata tttacaagac aagggaaagg tgactgttaa tgatttggct caggctttgg
    49861 gaaaagacag ttccaaggat tttcgtgagt tgattaaaac cttgtcctta atggaaagaa
    49921 agcaccaaat tcgttttgaa gaagatggta gtctgacatt agaaattaag aaaaaacatg
    49981 agattaccct caaggggatt tttcatgccc ataaaaatgg ctttggcttt gttagtctgg
    50041 aaggcgagga ggacgacctt tttgtaggga aaaatgatgt caactatgct attgatggtg
    50101 ataccgtcga ggtagtgatt aagaaagtcg ctgaccgcaa taagggaaca gcagcagaag
    50161 ccaaaattat tgatatccta gaacacagtt tgacaacagt tgtcgggcaa atcgttctgg
    50221 atcaggaaaa acctaagtat gctggctata ttcgttcaaa aaatcagaaa atcagtcaac
    50281 cgatttatgt taagaaacca gccctaaaat tagaaggaac agaagttctc aaggtcttta
    50341 tcgataaata cccaagcaag aaacatgatt tctttgtcgc gagtgttctc gatgtggtgg
    50401 gacactcaac ggatgtcgga attgatgttc ttgaggtttt ggaatcaatg gacattgtat
    50461 ccgagtttcc agaagctgtt gttaaggaag cagaaagtgt gcctgatgct ccgtctcaaa
    50521 aggatatgga aggtcgtctg gatctaagag atgaaattac ctttaccatt gacggtgcgg
    50581 atgccaagga cttggacgat gcagtgcata tcaaggctct gaaaaatggc aatctggagc
    50641 ttggggttca catcgcagat gtttcttatt atgtgaccga ggggtctgcc cttgacaagg
    50701 aagcccttaa ccgtgcgact tctgtttacg tgacagaccg agtggtgcca atgcttccag
    50761 aacgactatc aaatggcatc tgctctctca atccccaagt tgaccgcctg acccagtctg
    50821 ctattatgga gattgataaa catggtcgtg tggtcaacta taccattaca caaacagtta
    50881 tcaagaccag ttttcgtatg acctatagcg atgtcaatga tatcctagct ggcgatgaag
    50941 aaaagagaaa agaatatcat aaaattgtac caagtatcga actcatggcc aagcttcatg
    51001 aaactttaga aaacatgcgt gtgaaacgtg gagctctcaa ttttgatacc aatgaagcga
    51061 agattttagt ggataaacaa ggtaagcctg ttgatatcgt tcttcggcag cgtggcattg
    51121 ccgagcggat gattgagtct tttatgttga tggctaatga aacagttgcc gaacatttca
    51181 gcaagttgga tttgcctttt atctatcgaa ttcacgagga gcctaaggct gaaaaggttc
    51241 agaagtttat tgattatgct tcgagttttg gcttgcgcat ttatggaact gccagtgaga
    51301 ttagtcagga ggcacttcaa gacatcatgc gtgctgttga gggagaacct tatgcagatg
    51361 tattgtccat gatgcttctt cgctctatgc agcaggctcg ttattcggag cacaatcacg
    51421 gccactatgg actagctgct gactattata ctcactttac cagtccaatt cgtcgttatc
    51481 cagaccttct tgttcaccgt atgattcggg attacggccg ttctaaggaa atagtagagc
    51541 attttgagca agtgattcca gagattgcga cccagtcttc caaccgtgaa cgtcgtgcca
    51601 tagaagctga gcgtgaagtc gaagccatga aaaaggctga gtatatggaa gaatacgtgg
    51661 gtgaagagta tgatgcagtt gtatcaagta ttgtcaaatt cggtctcttt gtcgaattgc
    51721 caaacacagt tgaaggcttg attcacatca ctaatctacc tgaattttat catttcaatg
    51781 agcgtgattt gactcttcgt ggagaaaaat caggtatcac tttccgagtg ggtcagcaga
    51841 tccgtatccg tgttgaaaga gcggataaaa tgactggaga gattgatttt tcattcgtac
    51901 ctagtgagtt tgatgtgatt gaaaaaggct tgaaacagtc tagtcgtagt ggcatagggc
    51961 gtggttccaa tcgtcgttcg gataagaagg aagacaagag aaaatcagga cgctcaaatg
    52021 ataagcgtaa gtattcacaa aaagacaaga agaaaaaagg aaagaaacct ttttacaagg
    52081 aagtagctaa gaaaggagcc aagcatggca aagggcgagg gaaaggtcgt cgcacaaaat
    52141 aaaaaggcac gccacgacta tacaatcgta gatacgctag aggcagggat ggtcctgact
    52201 ggaactgaaa tcaagagtgt acgagctgct cgaattaatc tcaaggatgg ctttgctcaa
    52261 gtgaaaaatg gagaagtttg gctgagcaat gttcatatcg cgccttacga agagggcaat
    52321 atctggaacc aggaaccaga acgtcgtcgt aaactcctgc tccataaaaa gcaaattcaa
    52381 aaattggaac aagagaccaa agggacagga atgaccttag ttccccttaa ggtctatata
    52441 aaagatggct acgctaagct tcttttagga cttgccaaag ggaagcatga ctatgacaaa
    52501 cgggagtcta tcaaacgtcg tgagcaaaat cgagatatcg cgcgtgtgat gaaagctgtt
    52561 aatcagcgat aaaaagagga attgaaaatg gaaaaattag ttgcctataa acgcatgcct
    52621 ttgtggaata aacaaacaat gcctgaagct gttcagcaaa agcacaatac aaaagttggg
    52681 acttggggga aaattactgt cttgaaggga gctctcaagt ttattgaatt gacagaagaa
    52741 ggggaagttc tagctgaaca cctctttgaa gcaggggcag acaatccaat ggcccaacct
    52801 caagcctggc accgagtgga agctgccaca gatgatgtgg aatggtactt ggaattttat
    52861 tgtaaacctg aggattattt tgctaaaaaa tacaatacca atcctgttca ttcagaggtc
    52921 ctagaggcca tgcagacagt gaaacaaggg aaagctttgg atttgggttg tggtcagggg
    52981 cgtaattctc tttttctagc ccagcaagat tttgatgtga cggctgtaga tcaaaatgga
    53041 ctagctcttg aaatcttgca aagcattgtg gagcaggaag atttggacat gcctgttggc
    53101 ctttacgata tcaattcagc tagcattgaa caagaatatg attttatcgt ttcaacagtt
    53161 gttctcatgt ttctacaagc ggaccgcatt ccagctatta ttcaaaatat gcaggagaaa
    53221 accagtgttg gtggttacaa ccttatcgtt tgtgccatgg acacggagga ttatccttgc
    53281 tcggttaact tcccattcac ctttaaagaa ggagaactgg cagactatta caaggattgg
    53341 gaattggtta agtacaatga aaatccaggc catttgcacc gtcgcgatga gaatggcaat
    53401 cgtattcaac tacgctttgc gaccttacta gctaagaaaa tcaagtaaac atacatgaag
    53461 attaggaatt ttcctgatct tttttctttt ttacgaatga tatagaaaag gagggaattc
    53521 atgtttgttg cgagagatgc taggggagaa ttggtaaatg tgttagagga taaacttgag
    53581 aagcaagcat acacctgccc agcttgtgga ggccagatcc atttgcgtca aggaccaagt
    53641 gtacggacgc attttgccca taaatcctta aaagactgtg attttttctt tgaaaatgaa
    53701 agtccagaac acctggccaa taaggaatcc ctctatcact ggttgaaaaa agagacaaag
    53761 gttcaattag agtacccgct ttcagaactt aaacagattg cggatgtatt tgtaaatggc
    53821 aatctagctc tagaagttca gtgtagtccc ttgcctcaga aagtccttaa agagcgaagt
    53881 gagggctatc gtagtcaggg ttaccaagta ctgtggttgc tgggtcaaaa actgtggctc
    53941 aaggagcgtt tgactcgtct acagcaaggt tttctttatt tcagtcaaaa catgggcttt
    54001 tatgtttggg aattagacaa ggaaaaacaa gttttaagac tcaaatacct gatttaccag
    54061 gatctccgcg gtaaactcca ttatcaaatc aaggaatttt cctatggtca aggtagttta
    54121 ttggaaatat tgcgtcttcc ctataagaga caaaaaatat ctcattttac agtttctgag
    54181 gacaaggaca tctgtcgcta tatccggcaa caactttatt atcaaaatct cttttggatg
    54241 aaagaacaag cagaagccta tcaaaaggga gaaaatatcc tgacttatgg actgaaagaa
    54301 tggtatccac aaattcgacc aatagtgggc aaatttttcc agattgaaca agacttgact
    54361 agctattatc agcactttta tacctattac caaaaaaatc ctcaaaatga ttggcaaaag
    54421 ctttatccac cagcctttta tcagcaatat ttcttgaaaa atatggtaga atagaaagga
    54481 tggaggaatc taatggtatt acaaagaaat gaaataaatg aaaaagatac atgggatcta
    54541 tcaacgatct acccaactga ccaggcttgg gaagaagcct taaaagattt aacagaacaa
    54601 ttggagacag tagcccagta tgaaggccat ctcttggata gtgcggataa cctactagaa
    54661 atcactgaat tttctcttga aatggaacgc cagatagaga agctttacgt ttatgctcat
    54721 atgaagaatg accaggatac acgtgaagct aagtatcaag agtactatgc caaggccatg
    54781 acactctaca gccagttaga ccaagccttt tcattctatg agcctgaatt tatggagatt
    54841 agcgaaaagc agtatgctga ctttttagaa gctcaaccaa agctgcaggt ttatcaacac
    54901 tattttgaca agctcttgca aggcaaggat cacgttcttt cacaacgtga agaagaatta
    54961 ttggctggag ctggagaaat ctttggttca gcaagtgaaa ccttcgctat cttggacaat
    55021 gcggatattg tgttccctta tgtcctagac gatgatggta aagaagttca gctatctcat
    55081 gggacttaca cacgtttgat ggagtctaaa aaacgtgagg ttcgccgtgg tgcctatcaa
    55141 gctctttatg cgacttacga acaattccaa cacacctatg ccaaaacctt gcaaaccaat
    55201 gttaaggtgc aaaattaccg tgctaaagtt cgtaactaca agagtgctcg tcatgcagcc
    55261 ctcgcagcga attttgttcc agaaagtgtt tatgacaatt tggtagcagc agttcgcaag
    55321 catttgccac tcttacatcg ctatcttgag cttcgttcaa aaatcttggg gatttcagat
    55381 ctcaagatgt acgatgtcta cacaccgctt tcatctgttg aatacagttt tacctaccaa
    55441 gaagccttga aaaaagcaga agatgctttg gcagtcttgg gtgaggatta cttgagccgt
    55501 gttaaacgtg ccttcagcga gcgttggatt gatgtttacg aaaatcaagg caagcgttca
    55561 ggtgcctact ctggtggttc ttacgatacc aatgccttta tgcttctcaa ctggcaggac
    55621 aatctggaca atctctttac tcttgttcat gaaacaggtc acagtatgca ttcaagctat
    55681 actcgtgaaa ctcagcctta tgtttacggg gattactcta tctttttggc tgagattgcc
    55741 tcaactacca atgaaaatat cttgacggag aaattattgg aagaagtgga agacgacgca
    55801 acacgctttg ctattctcaa taacttccta gatggtttcc gtggaacagt tttccgccaa
    55861 actcaatttg ctgagtttga acacgccatt caccaagcag atcaaaatgg ggaggtcttg
    55921 acaagcgatt tcctaaataa actctacgca gacttgaacc aagagtatta tggtttgagt
    55981 aaggaagaca atcctgaaat ccaatacgag tgggctcgca ttccacactt ctactataac
    56041 tactatgtat atcaatattc aactggcttt gcggccgcct cagccttggc tgaaaaaatt
    56101 gtccatggta gtcaagaaga ccgtgaccgc tatatcgact acctcaaggc aggtaagtcg
    56161 gactatccac ttaatgtcat gagaaaagct ggtgttgata tggagaagga agactacctc
    56221 aacgatgcct ttgcagtctt tgaacgccgt ttaaatgagt ttgaagccct tgttgaaaaa
    56281 ttaggattgg cataaaatgg ttgaatcgta tagtaagaat gctaaccata acatgcgtcg
    56341 tcctgtcgtc aaagaagaaa ttgtagactt gatgcgtcag cgtcaaaagc aggtcacagg
    56401 tttcttgaaa gaattggaag actttgcccg caaggaaaat attcctatta ttccccatga
    56461 aacggttgct tatttccgtt ttcttatgga aaccatgcag cctaaaaata ttctggaaat
    56521 tgggacggct atcggttttt cagctctctt gatggctgaa catgcgccaa atgctaagat
    56581 tacaactatt gatcgtaatc cagaaatgat tggttttgcc aaggaaaatt ttgcccagtt
    56641 tgacagtcgc aagcaaatca ctctcctaga gggagatgcg gtggatgtct tatctacact
    56701 gacagagtct tatgatttcg tatttatgga ttctgccaag tctaaataca tcgtctttct
    56761 gccagaaatc ctcaaacatt tggaagttgg tggtgtggtt gtcttggatg atatttttca
    56821 aggtggtgat gttgccaagg atattatgga agtccgtcgt ggtcagcgaa ccatttatcg
    56881 aggccttcaa aaattatttg atgcaacctt agacaatcca gaactcaccg caacattagt
    56941 gcctttagga gatggtattc tcatgcttcg taaaaatgta gcagatgttc aactgtctga
    57001 aagcgaatga ttttcagaaa aatttaagaa aaaatagtaa aatagataga gtaacactta
    57061 tctcaaagga gtagacatga agaaaaaatt attggcaggt gccatcacac tattatcagt
    57121 agcaacttta gcagcttgtt cgaaagggtc agaaggagca gaccttatca gcatgaaagg
    57181 ggatgtcatc acagaacatc aattttatga gcaagtgaaa aacaaccctt cagcccaaca
    57241 agtcttgtta aatatgacca tccaaaaagt ttttgaaaaa caatatggct cagagcttga
    57301 tgataaagag gttgatgata ctattgccga agaaaaaaaa caatatggcg aaaactacca
    57361 acgtgtcttg tcacaagcag gtatgactct tgaaacacgt aaagctcaaa ttcgtacaag
    57421 taaattagtt gagttggcag ttaagaaggt agcagaagct gaattgacag atgaagccta
    57481 taagaaagcc tttgatgagt acactccaga tgtaacggct caaatcatcc gtcttaataa
    57541 tgaagataag gccaaagaag ttctcgaaaa agccaaggca gaaggtgctg attttgctca
    57601 attagccaaa gataattcaa ctgatgaaaa aacaaaagaa aatggtggag aaattacctt
    57661 tgattctgct tcaacagaag tacctgagca agtcaaaaaa gccgctttcg ctttagatgt
    57721 ggatggtgtt tctgatgtga ttacagcaac tggcacacaa gcctacagta gccaatatta
    57781 cattgtaaaa ctcactaaga aaacagaaaa atcatctaat attgatgact acaaagaaaa
    57841 attaaaaact gttatcttga ctcaaaaaca aaatgattca acatttgttc aaagcattat
    57901 cggaaaagaa ttgcaagcag ccaatatcaa ggttaaggac caagccttcc aaaatatctt
    57961 tacccaatat atcggtggtg gagattcaag ctcaagcagt agtacatcaa acgaatagtc
    58021 caaatcaatg agtcagggaa aaaactctac ttcaggaaaa aatgaagcaa acattcccac
    58081 aataaaacgc gtagtacaag gtttgtactg acccccaaaa gttagacaaa taatttatcc
    58141 gaaggattta gttctgtatt gcacagggct aagtcctttt agttttacct taattctctt
    58201 gttgttgtaa taatcaatat agtctataat ggcttgttcc aattgattaa gtgatttaaa
    58261 tgttttctca tagccataaa acatttcgga ttttaaaata ccaaagaaag attccatcct
    58321 accgttgtct tggctgtttc ccttgcgtga catagatgct tgaattccct tactctctag
    58381 gaaccgatga taagaatcgt gttggtattg ccagtcttgg tcactatgga gaatcgtatt
    58441 ctcgtagtgc ttctctttga atgcctgttc caacattaac gatcaatcaa tttaatcatg
    58501 tacctaatat tagaattgtt tatctcaaat ttatttgaaa gcttctctaa gctatatcct
    58561 tgttttctaa gttcatagat ctgaacttta tcatcataag ttaatttcat aataaaaaca
    58621 ctccaaaagt tagatttttt ctgtctaact tttggggtgc agttcattta acacctgata
    58681 atatgcgttt tttttatttt aaagactttt tgaccagcct cattttttaa cttgatactc
    58741 agtgaaaagc aaagattaaa ctaggaagct agctgtaggc tgctcaaaga acagctttga
    58801 ggttgcagat aaaacttgtg aggtaaccaa catatataat gtgaagctga cgtggtttga
    58861 atagatttta gaagggtatg agtctggaag ttttaatgga taatgcaaga ttccatagaa
    58921 tgggtaagct agagttctta tgtgaagagt ttgggcataa acttttacct tttcctccct
    58981 actcatctta gtatagaaaa gtgaatctaa aatagtacat aactgcttct aaaacattct
    59041 tataaattga tttaaattct caaatcatat tattcagttc ttatttcatt ttgctctaca
    59101 atcctgttga gaagacacgt gttcatatca aaaaaggtat tggcaagttg caataccttt
    59161 ttacgaggct cttttatctt atttttgttt caactgacta tatctcctat ggttctagtt
    59221 cagaaggcta ggctataatt atgattgata agaagtatca ttccaagtat tgagagtgaa
    59281 tgtttcaaaa tcatgggttt ctataatggt caggctggca tttgctagac cgccatcttt
    59341 acgaagaagt ggttctttat agcctaggag agtacgaaga ctggcagtaa gattggcgcc
    59401 gtgtccgaca attagaatac gttcagctgg actatctttt aatgatttga taaattggat
    59461 ggtccgttga gttgtactat agagggattc ggctccgaac attcgagtgt caaattgagc
    59521 aagatttgaa cgaaaagcct ggatttgttg cgggtaaata gcttccaagg ttgcaatttt
    59581 caaaccttct aacttcccaa gttgccattc acggagatta ggaacgattt ctaaagaaca
    59641 gggggtatag agttgacttt ggataatctc agcagatttg accgctcgag gtaaatcact
    59701 tgaataaatc tgatcaaaag gaatttcctt gagatactga ccaagtcgtt ttagggtttc
    59761 aatggattca ggaagaagag gagaatcacc actagcacct tgaaaacgac cttcttggtt
    59821 ccagagggta cgaccgtggc ggacaaagta gagtttcatt acttgatgtc ctccaaaata
    59881 tctacaaagt ctgcctttac aaagctagcc aagtcttgtg gcgcgacgat aatgctgtgt
    59941 ccgacttcgc ctgcagagac aatcatttga tccaaatcta gagcaatttt atcgataaaa
    60001 atgggataat tgtgtttctg acgaattccg acaggattat tggctccatg aatgtaacca
    60061 gttgtttttt ctaagtcctt ttgtggaatc atgctcactt ttttattgcc agaaatttta
    60121 gctagtttct tttcagacaa gtgctgagtg atagggacaa ttccgataat cggtccggtc
    60181 ttgtctccca aaagcgccaa ggttttgaaa atctgatctc gttcataacc ttgaggaagc
    60241 tctccttcta gggcattgat ttgaattccc tgatgaggga tagctgcttt agataggatt
    60301 tgttccacca atgttttttt gattttaact ttttttgcca ttatttatat ttatcctcca
    60361 attgactcat ccaaatacca agccagattc ccagcgcaaa gaagaaggcg atgatgacat
    60421 aaccgacaag tgaaagtcct gtgtattgga tactttcagc gtttcctgca tttggaatta
    60481 agatcaaaag ggtacttgat aggacgatac cgatgatgaa atgatagacg cgtgagtggt
    60541 agttgtttaa ggcataatcc atcaattttg aaaaaacgat gagagttgca cctgcaccaa
    60601 ttccaatcgg aaagaaggtt cccaagagat caaaagtttt aaaaccagtc aacataggag
    60661 catagagtcc caaaatcaaa agtaaatttg atgggctgag gccaggaacc aagacgccaa
    60721 gggccaatag tgcgcctgct aggacgaagt taagaaagct ggcgcttaag gttccaacga
    60781 caaaatttaa ggcatagagt cctaatccag aaatgataaa ggttgtccat aaccaagtta
    60841 aatcaatctt gtctcggtca gattctcgag ttgattcttt gaggaggcta ggaactgtac
    60901 cgataatagc tcccgcaaag ctccataata caaagacctg ataattttca agcaggtatt
    60961 caatcgggta ggaaaataag ccgattccta gaagcatacc gatggcaact ggaataaagt
    61021 acaaaacatt ttctttaaag tctttaaagg gatgggccag aaagccaatc attcgttcat
    61081 agattcctaa gattgctgct agagcccctc cggaaattcc cggtaggata aatccaagag
    61141 caattacaat ccctttaata acgcgtgcta accatgagag catatatttc ctccaactat
    61201 ttctatttca aaaaatcctt actatattgt atcacaatca gacacaaaaa gaaaaagcaa
    61261 atgataaaca aatgctttta aggttttaaa aaaagttttc gaaaggtttc ttctttattt
    61321 tttaagggag agataacgtt gatatctaaa tcgtggtcaa agccggcaat ttttccttta
    61381 gatgtgtatt ggtgaatatc ataatctaaa tcagttttag gactgctctc caaaaatcct
    61441 gagtctgagc cgtaggacgg aatccaaaca gaggtaaact tgcctgtatc aatactgtgt
    61501 tcttccatga agtagacacc aacgtagatg ccgatgtttt tagcacctag tgatgctagt
    61561 tttgctcgaa agttttcgac accttcgttc atattagaca tggttttgtc ttccacgtca
    61621 agccaatagt aactagggct gtaaggagag gcagcattgt agaaaacttc ggcagccttt
    61681 tccatttctt ggacactttt tccagctaca taagcgtaga cagcaactgg gacattccgc
    61741 ttttgaagtt cagtgatatg actcttatag gccttgtcta ttccattgat aaatgaagca
    61801 tcattttctt ttgtcgtttg agcaccactg tgaacacgaa caatagcacc tgaaatattt
    61861 tgtgagaggg catcgtagtt gatttcttca ggacgctgcc agccagagag gtcaataatc
    61921 ggtttgtcta agtgtttcaa agcctgtgct tcaatctgtg ctatattgga ttttgtttta
    61981 aacgattggt tgtcattaag tgggcgattg atgattaaaa tgaacatcat aatccccaaa
    62041 aaactaaata aaataagtgg atgaatttgt tttctcatat cttataattc taccctaaaa
    62101 atcaaaaaaa atcaaaaaaa tgggttaagg aagagacttt agagcatttt ttcattcaag
    62161 agtgcggaat gatttgaaat atggtataat aaaagggaat ttctacagaa aagagaagat
    62221 tatgtcaaat tttgccatta ttttagcagc gggtaaaggg actcgcatga aatctgattt
    62281 gccaaaagtt ttgcacaagg ttgcgggtat ttctatgttg gaacatgttt tccgtagtgt
    62341 gggagctatc caacctgaaa agacagtaac agttgtagga cacaaggcag aattggttga
    62401 ggaggtcttg gctggacaga cagaatttgt gactcaatct gaacagttgg gaactggtca
    62461 tgcagttatg atgacagaac ctatcttaga aggtttgtca ggacacacct tggtcattgc
    62521 aggagatact cctttaatca ctggtgaaag cttgaaaaac ttgattgatt tccatatcaa
    62581 tcataaaaat gtggccacta tcttgactgc tgaaacggat aatccttttg gctatggacg
    62641 aattgttcgt aatgacaatg ctgaggttct tcgtattgtt gagcagaagg atgctacaga
    62701 ttttgaaaag caaatcaagg aaatcaacac tggaacatac gtctttgaca acgagcgttt
    62761 gtttgaggct ttgaaaaata tcaataccaa taacgctcaa ggcgaatact atattacaga
    62821 cgtcattggt attttccgtg aaactggtga aaaagttggc gcttatactt tgaaagattt
    62881 tgatgaaagt cttggggtaa atgaccgtgt ggcgcttgcg acagctgagt cagttatgcg
    62941 tcgtcgcatc aatcataaac atatggtcaa cggtgttagc tttgtcaatc cagaagcaac
    63001 ttatatcgat attgatgttg agattgctcc ggaagttcaa atcgaagcca atgttacctt
    63061 gaaagggcaa acgaaaattg gtgctgagac tgttttgaca aacggtactt atgtagtgga
    63121 cagcactatc ggagcaggag cggtcattac caattctatg attgaggaaa gtagtgttgc
    63181 agacggtgtg acagtcggtc cttatgctca cattcgtcca aattcaagtc tgggtgccca
    63241 agttcatatt ggtaactttg ttgaggtgaa aggatcttca atcggtgaga ataccaaggc
    63301 tggtcatttg acttatatcg gaaactgtga agtgggaagc aacgttaatt tcggtgctgg
    63361 aactattaca gtcaattatg acggcaaaaa caaatacaag acagtcattg gagacaatgt
    63421 ctttgttggt tcaaattcaa ccattattgc accagtagaa cttggtgaca attccctcgt
    63481 tggtgctggt tcaactatta ctaaagacgt gccagcagat gctattgcta ttggtcgcgg
    63541 tcgtcagatc aataaagacg aatatgcaac acgtcttcct catcatccta agaaccagta
    63601 ggagcctatc atggagtttg aagaaaaaac gcttagccga aaagaaatct atcaaggacc
    63661 aatatttaaa ctggtccaag atcaggttga attaccagaa ggcaagggaa ctgcccaacg
    63721 ggatttgatt ttccacaatg gggctgtctg tgttttagca gtaacggatg aacaaaaact
    63781 gatcttggtc aagcagtacc gcaaagctat cgaggctgtc tcttacgaaa ttccagccgg
    63841 aaaattggaa gtaggagaaa acacagcccc tgtggcagct gcccttcgtg aattagagga
    63901 agaaacagcc tatacaggga aattagaact cttgtacgat ttttattcag ctattggctt
    63961 ttgtaatgag aagttaaaac tatatttagc aagcgatttg acaaaagtgg aaaatccgcg
    64021 tccgcaggat gaggatgaaa ccttggaagt ccttgaagtg agcttagaag aagcgaaaga
    64081 attaatccaa tcaggtcata tttgtgatgc caagacaatt atggctgttc agtattggga
    64141 gttgcagaaa aaatagagga ggtcagtatg ggtaaatctt tattaacgga tgaaatgatt
    64201 gaaagagcta atagaggcga aaaaatttca ggtcctcctt tgctagatga taatgaggag
    64261 actaagattt taccaacctc ttcttcccgt tttggttatg ccaatcctaa gaatcatggt
    64321 tttagccagg aaaccttgaa gattcaggtc gaaccatcta ttcataaaag ccgtcgcatt
    64381 gaaaatacca agagaaatgt cttcaattct aagttgaata aaatcttatt cgcggtcatc
    64441 tttctcttga ttttgcttgt tttagcaatg aaacttttgt aatagaaaag gaattgaaat
    64501 gaaaatagga attattgctg ctatgccaga agaactggct tatctggtcc agcatttata
    64561 taatgtccag gagcaagttg ttttggggaa tacctatcat acaggaacca ttgcttctca
    64621 tgaagtcgtt cttgtagaaa gtggaattgg taaggtcatg tctgctatga gtgtggcgat
    64681 tttggctgat catttccagg tggatgccct tattaatacg ggttcagctg gggcagtagc
    64741 agaaggtatc gctgttgggg atgtcgtgat tgctgacaaa ttagcctatc atgacgtgga
    64801 tgtcacagct tttggctatg cttatggaca aatggcgcaa caaccgcttt atttcgaatc
    64861 agacaaaacc tttgttgctc aaatccaaga gagtttatct caattggacc aaaactggca
    64921 tcttggtttg attgctacag gagatagttt tgttgcagga aatgacaaga tagaagcgat
    64981 taagtcccat ttcccagaag ttttagccgt ggagatggag ggggcagcta ttgctcaagc
    65041 agcgcatacc ctcaatctcc cagtcttagt catccgagct atgagtgaca atgccaacca
    65101 tgaagcaaac atcttttttg atgagtttat tatcgaagct ggacgtcgct ctgcccaagt
    65161 cttgttggcc tttttgaagg ctttagatta agcggaaatt tgacagtttt tctagcttat
    65221 gataagattt aagtaaagaa aagctagaaa acgtttcaga ggatattatg agtattgaaa
    65281 tgaccgtcag tgagattgca gaggtcttag gattatctcg ccaagcaatc aataaccgtg
    65341 tcaaagaatt accagaagaa gacacagata aaaatgacaa aggggtaaca gttgttacca
    65401 gaagtggctt gattaagcta gaagaaatct ataaaaaaac gatttttgaa gatgaacctg
    65461 tcagtgaaga tgtcaaacaa cgtgaactga tggagattct agtggatgag aagaatgcag
    65521 aaattcttcg tctttatgaa caattaaaag ccaaggatcg tcagttatca gaaaaagacg
    65581 agcagatgcg tatcaaagac cgtcagattg ctgagaaaga caaacaatta gatcagcaac
    65641 aacaattgac ccttcaagcc atgaaggatc aagaaaatct taagctagag ctggaccaag
    65701 caaaagaaga agtccaatcc actaaaaaag gcttttttgc tcgtttattt ggaggataat
    65761 caaggagctg tttaggttaa atgctaacct gatggcttct tttgtttgtg aatagtatag
    65821 ttgattagaa cagagacagt agaggagagg tacagaatgg aacgattaga agattacatt
    65881 gcttttgatt tagaatttaa tcagcacgaa ggacaaacac acttgattca agtatcggca
    65941 gtgcgtttta gagatggtcg ggaaatcgat gcctatgatt cctatgttca taccagcgtc
    66001 cctctaaaaa gctttatcaa tggtttgacc gggattacag ctgagacctt aaaagatgct
    66061 cctccagttg agaaagtctt agaagaattc caagagtttg tgggttcttt gctgatgata
    66121 ggctacaatg ctgctaaaag tgatttacct attttagcag agcatggttt agactatagc
    66181 caacagtaca aggtggattt gtatgatgaa gcttttgaac gtcggagttc agatttacat
    66241 ggtattgcca atctcaaatt gcaaactgtt gcctcatttc ttggatttca aggtcagtct
    66301 cataatagtt tagaggatgc tcggatgacg gcgcgtgttt acgaatcctt cttagagtcg
    66361 gatcaagcta gggaattgtt agagacagaa agtagcttat caaacaatcc ttttggtggc
    66421 ttggacttgt ctcaattatt tgactaggag tgcctatgaa acctgaaaaa cctaaaaaat
    66481 tgggtcttaa acagatttac ctcaatcttg ataaaatcct ctttctattt ttcttgattt
    66541 ttatgatagt ggagtatttg tgggtaccat tcaattcatg gttggctagc ttcttattag
    66601 atcagacagg ttatcttttt atttcctata acaatgtctt agccatcctt actcattctc
    66661 cattgattag tctagctttt ttagccttga ttgtaattaa ctttctggtt gcttattttc
    66721 aaataggact cctctttatt ggtgctcgtc atctcctcta taatgaaaag cgaaggctaa
    66781 ttgagtatat ccgcaaggtt ttccatgaaa gttttgaatt tatgaaaaag ctaactggcg
    66841 ctaaagcctt gtttatcttt ttctatatag ctatgctgtt tccttttata cggaaaatgc
    66901 tgaagattta ttatcttaat aaaatcgtca ttccagagtt tatccaagct tatttagaag
    66961 ataaatattg gatgtggtgg ctgagcatcc tacttctatc tcttatcttt ctctatgtct
    67021 ctgtcagatt gatgtttgcc cttccgaaga ttgtatatga tcagctgact gttcgagaag
    67081 cagtgatgtt tagcttggag aaaaccaaga agagagttac tttttatgct tggaatttat
    67141 tttacatctt gctcaaagca catttattat tttatcttcc tttgattcct ttattattgg
    67201 ctcaaacttt ggtagatgat atcactcaga gagagtctct gattcttggt attttgaatt
    67261 ttgtcgtgat taaaaatctc tattatatgg ctctgactta ttttttggtt aaatttgttt
    67321 catttttgac aggaaaggag ctggatatgc ttcctaggag agaaaaagat cacatcgtgc
    67381 gatggggtgt catgacttgt gccagttttt tctttgcctt agaaggttat atttatctgg
    67441 aggctcccat ggttcatcta cctcaactta tttctcaccg aggggtttcc aatgcaaatg
    67501 ggattcaaaa tacagtagag tccttggaaa ctacagcaca actcaaacca gacttggtgg
    67561 agacggacgt gcaggaaaca aaagatgggc agtttgtcat gatgcatgat gctaacttga
    67621 aaaatctagc aggtatcaat aaaagtcctc aagacttaaa cttggaggag cttaaaggga
    67681 ttgatatttt tgaaaatggc taccagacta aaatttcaag ctttgaagat tatctcagtc
    67741 gagccaacga acttggtcaa aaattactaa ttgaaattaa aaccagtaaa aaagatagtc
    67801 cagacatgat gaaccgcttt ttagcccgtt atgctgcaaa gctcaagatt tatggacatc
    67861 aaatccagtc tttagactac catgttgtcg aaaaagtaag acagtatgat gccgaactgc
    67921 cagtttattt catcatgccc tacaattctg tctttcctaa aacaagagcg acaggatata
    67981 ctatggagta ctcaaccttg gatgaatatt ttgtaagcaa actatggaca acggatcaga
    68041 aactttatgt ctggactgtc aatgattcag aagctatcag caaatctctt cacttaggag
    68101 tagatggagt gattacagat gatttggaaa aagttcagcg ggaaatagaa gtagcccaag
    68161 aagatccaga atatacggat ttgctcttga aaaaagcatt ggaattcttt gaattttagt
    68221 aataaaaaag aggaacggtt gttcctccca tgataggtaa gtattcttgt gaatgcttgc
    68281 ttttttgtgg ttgcaatccg tttacgagaa ttgtttgtat tgttcttgtt tcaatttact
    68341 ttatagtgct ttgaagttgg aatgctacac tataacttct aaaatatagt aaaatgaacc
    68401 aaaaat
//
LOCUS       DAVKLX010000003        68404 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKLX010000003 DAVKLX010000000
VERSION     DAVKLX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 68404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.003"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..134)
                     /locus_tag="VW325_000209"
     CDS             complement(<1..134)
                     /locus_tag="VW325_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9733685.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIARLDYDTPSCP
                     "
     gene            327..824
                     /locus_tag="VW325_000210"
     CDS             327..824
                     /locus_tag="VW325_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9733686.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            824..2479
                     /gene="dnaX"
                     /locus_tag="VW325_000211"
     CDS             824..2479
                     /gene="dnaX"
                     /locus_tag="VW325_000211"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9733687.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2507..2701
                     /locus_tag="VW325_000212"
     CDS             2507..2701
                     /locus_tag="VW325_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9733688.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2851..3621
                     /gene="sufC"
                     /locus_tag="VW325_000213"
     CDS             2851..3621
                     /gene="sufC"
                     /locus_tag="VW325_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9733689.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3649..4911
                     /gene="sufD"
                     /locus_tag="VW325_000214"
     CDS             3649..4911
                     /gene="sufD"
                     /locus_tag="VW325_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9733690.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4922..6148
                     /locus_tag="VW325_000215"
     CDS             4922..6148
                     /locus_tag="VW325_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9733691.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPLKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            6135..6575
                     /locus_tag="VW325_000216"
     CDS             6135..6575
                     /locus_tag="VW325_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9733692.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6629..8041
                     /gene="sufB"
                     /locus_tag="VW325_000217"
     CDS             6629..8041
                     /gene="sufB"
                     /locus_tag="VW325_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9733693.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFGDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFNQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8272..9513)
                     /locus_tag="VW325_000218"
     CDS             complement(8272..9513)
                     /locus_tag="VW325_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9733694.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNTDHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9616..10725
                     /locus_tag="VW325_000219"
     CDS             9616..10725
                     /locus_tag="VW325_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9733695.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11259..11606
                     /gene="ssrA"
                     /locus_tag="VW325_000220"
     tmRNA           11259..11606
                     /gene="ssrA"
                     /locus_tag="VW325_000220"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11789..11876)
                     /locus_tag="VW325_000221"
     tRNA            complement(11789..11876)
                     /locus_tag="VW325_000221"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11840..11842),aa:Ser,seq:gga)
     gene            12038..12778
                     /locus_tag="VW325_000222"
     CDS             12038..12778
                     /locus_tag="VW325_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9733696.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12775..13686
                     /gene="pfkB"
                     /locus_tag="VW325_000223"
     CDS             12775..13686
                     /gene="pfkB"
                     /locus_tag="VW325_000223"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9733697.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            13683..15635
                     /locus_tag="VW325_000224"
     CDS             13683..15635
                     /locus_tag="VW325_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9733698.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15799..18024
                     /locus_tag="VW325_000225"
     CDS             15799..18024
                     /locus_tag="VW325_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9733699.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(18177..18605)
                     /locus_tag="VW325_000226"
     CDS             complement(18177..18605)
                     /locus_tag="VW325_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733700.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            18743..19885
                     /locus_tag="VW325_000227"
     CDS             18743..19885
                     /locus_tag="VW325_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9733701.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19894..21108
                     /gene="thiI"
                     /locus_tag="VW325_000228"
     CDS             19894..21108
                     /gene="thiI"
                     /locus_tag="VW325_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9733702.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            21243..22067
                     /locus_tag="VW325_000229"
     CDS             21243..22067
                     /locus_tag="VW325_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9733703.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMLYFLGNLYL"
     gene            22080..23246
                     /locus_tag="VW325_000230"
     CDS             22080..23246
                     /locus_tag="VW325_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9733704.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(23737..25407)
                     /locus_tag="VW325_000231"
     CDS             complement(23737..25407)
                     /locus_tag="VW325_000231"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9733705.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            25637..26326
                     /gene="coaB"
                     /locus_tag="VW325_000232"
     CDS             25637..26326
                     /gene="coaB"
                     /locus_tag="VW325_000232"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9733706.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKNLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            26338..26889
                     /gene="coaC"
                     /locus_tag="VW325_000233"
     CDS             26338..26889
                     /gene="coaC"
                     /locus_tag="VW325_000233"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9733707.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            26873..27436
                     /locus_tag="VW325_000234"
     CDS             26873..27436
                     /locus_tag="VW325_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9733708.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            27718..28245
                     /locus_tag="VW325_000235"
     CDS             27718..28245
                     /locus_tag="VW325_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9733709.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            28257..28772
                     /locus_tag="VW325_000236"
     CDS             28257..28772
                     /locus_tag="VW325_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9733710.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(28769..29236)
                     /locus_tag="VW325_000237"
     CDS             complement(28769..29236)
                     /locus_tag="VW325_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9733711.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(29310..29798)
                     /locus_tag="VW325_000238"
     CDS             complement(29310..29798)
                     /locus_tag="VW325_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9733712.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            complement(29764..30327)
                     /locus_tag="VW325_000239"
     CDS             complement(29764..30327)
                     /locus_tag="VW325_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9733713.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLVLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(30338..32326)
                     /gene="uvrB"
                     /locus_tag="VW325_000240"
     CDS             complement(30338..32326)
                     /gene="uvrB"
                     /locus_tag="VW325_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9733714.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(32403..33356)
                     /locus_tag="VW325_000241"
     CDS             complement(32403..33356)
                     /locus_tag="VW325_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9733715.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            33526..35691
                     /locus_tag="VW325_000242"
     CDS             33526..35691
                     /locus_tag="VW325_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9733716.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            35691..36431
                     /locus_tag="VW325_000243"
     CDS             35691..36431
                     /locus_tag="VW325_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9733717.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            36580..38067
                     /locus_tag="VW325_000244"
     CDS             36580..38067
                     /locus_tag="VW325_000244"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9733718.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFDAKWTWQPDIAYRQDGRFE"
     gene            complement(38111..39400)
                     /gene="ftsY"
                     /locus_tag="VW325_000245"
     CDS             complement(38111..39400)
                     /gene="ftsY"
                     /locus_tag="VW325_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9733719.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTVEENNSEVLEPERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRTGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(39404..40222)
                     /locus_tag="VW325_000246"
     CDS             complement(39404..40222)
                     /locus_tag="VW325_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9733720.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEKTE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVMPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(40222..41016)
                     /locus_tag="VW325_000247"
     CDS             complement(40222..41016)
                     /locus_tag="VW325_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9733721.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            complement(41013..44552)
                     /gene="smc"
                     /locus_tag="VW325_000248"
     CDS             complement(41013..44552)
                     /gene="smc"
                     /locus_tag="VW325_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9733722.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQDEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRQQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(44543..45241)
                     /locus_tag="VW325_000249"
     CDS             complement(44543..45241)
                     /locus_tag="VW325_000249"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9733723.1"
                     /translation="MKELQTVLKNHFAIEFTDKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTYLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(45424..45495)
                     /locus_tag="VW325_000250"
     tRNA            complement(45424..45495)
                     /locus_tag="VW325_000250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45460..45462),aa:Arg,seq:ccg)
     gene            complement(45559..46545)
                     /locus_tag="VW325_000251"
     CDS             complement(45559..46545)
                     /locus_tag="VW325_000251"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9733724.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            complement(46720..48036)
                     /locus_tag="VW325_000252"
     CDS             complement(46720..48036)
                     /locus_tag="VW325_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9733725.1"
                     /translation="MRITDNQYKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYQRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            complement(48023..49936)
                     /locus_tag="VW325_000253"
     CDS             complement(48023..49936)
                     /locus_tag="VW325_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9733726.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSINSRQNLSVNYDSGVSGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNDQ
                     TGQQDTGDNDADY"
     gene            complement(50102..50725)
                     /locus_tag="VW325_000254"
                     /pseudo
     CDS             complement(50102..50725)
                     /locus_tag="VW325_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            complement(50887..51246)
                     /gene="leuD"
                     /locus_tag="VW325_000255"
     CDS             complement(50887..51246)
                     /gene="leuD"
                     /locus_tag="VW325_000255"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9733727.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            complement(51250..51519)
                     /locus_tag="VW325_000256"
     CDS             complement(51250..51519)
                     /locus_tag="VW325_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9733728.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            complement(51516..52553)
                     /gene="leuB"
                     /locus_tag="VW325_000257"
     CDS             complement(51516..52553)
                     /gene="leuB"
                     /locus_tag="VW325_000257"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU9733729.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKHAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            complement(52565..53659)
                     /locus_tag="VW325_000258"
                     /pseudo
     CDS             complement(52565..53659)
                     /locus_tag="VW325_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
     gene            complement(53678..54334)
                     /locus_tag="VW325_000259"
     CDS             complement(53678..54334)
                     /locus_tag="VW325_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9733730.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            complement(54378..55010)
                     /locus_tag="VW325_000260"
     CDS             complement(54378..55010)
                     /locus_tag="VW325_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9733731.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            complement(55103..55462)
                     /locus_tag="VW325_000261"
     CDS             complement(55103..55462)
                     /locus_tag="VW325_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9733732.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYILMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            complement(55568..57655)
                     /gene="topA"
                     /locus_tag="VW325_000262"
     CDS             complement(55568..57655)
                     /gene="topA"
                     /locus_tag="VW325_000262"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9733733.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            complement(57822..58865)
                     /locus_tag="VW325_000263"
     CDS             complement(57822..58865)
                     /locus_tag="VW325_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9733734.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            complement(59754..60602)
                     /gene="dprA"
                     /locus_tag="VW325_000264"
     CDS             complement(59754..60602)
                     /gene="dprA"
                     /locus_tag="VW325_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9733735.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(60698..61387)
                     /locus_tag="VW325_000265"
     CDS             complement(60698..61387)
                     /locus_tag="VW325_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9733736.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(61399..62277)
                     /locus_tag="VW325_000266"
     CDS             complement(61399..62277)
                     /locus_tag="VW325_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9733737.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(62267..63136)
                     /gene="licA"
                     /locus_tag="VW325_000267"
     CDS             complement(62267..63136)
                     /gene="licA"
                     /locus_tag="VW325_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9733738.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIGSAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(63153..64175)
                     /locus_tag="VW325_000268"
     CDS             complement(63153..64175)
                     /locus_tag="VW325_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9733739.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLL
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(64180..64887)
                     /locus_tag="VW325_000269"
     CDS             complement(64180..64887)
                     /locus_tag="VW325_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9733740.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            65223..66710
                     /gene="tacF"
                     /locus_tag="VW325_000270"
     CDS             65223..66710
                     /gene="tacF"
                     /locus_tag="VW325_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9733741.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIIKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            66720..67523
                     /locus_tag="VW325_000271"
     CDS             66720..67523
                     /locus_tag="VW325_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9733742.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYELLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            67525..68334
                     /locus_tag="VW325_000272"
     CDS             67525..68334
                     /locus_tag="VW325_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9733743.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT        20212 a        14182 c        13090 g        20920 t
ORIGIN      
        1 tcagggcaag atggggtgtc ataatccagt ctagcgatga tttccttgtg tgtatcctta
       61 ttgatgatgt ctaaaatctg gatattaggg tctttaatgt ctagtaattt tgtgataaaa
      121 tgtaattgtt ctatatgatt ctttctaatg agttgttttg ttgcttttca ttataggtca
      181 tatgggactt tttttctaca caaaataggc tccataatat ccatagggga tttacccact
      241 acaaatatta tagagcccaa aatcctttgt ttactaaaca agggattttt cttttgtctc
      301 tgctcctttt ttgatataat agttctatgt taaaatcaga aaaacaatca cgttatcaaa
      361 tgttaaatga agaattgtcc ttcctattgg aaggcgaaac caatgttttg gctaatcttt
      421 ccaacgccag tgctctcata aaatcacgtt ttcctaatac cgtatttgca ggcttttatt
      481 tgttcgatgg aaaggaattg gttttaggcc ccttccaagg aggtgtttcc tgcatccgta
      541 ttgcactagg caagggtgtt tgtggtgagg cagctcactt tcaggaaact gttattgttg
      601 gagatgtgac gacctatctc aactatattt cttgtgatag tctagctaaa agtgaaattg
      661 tggtgccgat gatgaagaat ggtcagttac ttggagttct ggatctggat tcttcagaga
      721 ttgaggatta cgatgctatg gatcgagatt atttggaaca atttgtcgct attttgcttg
      781 aaaagacaac atgggacttt acgatgtttg aggaaaaatc ttaatgtatc aagcacttta
      841 tcgaaaatat agaagtcaaa acttctccca gttagttggt caagaagttg tggctaagac
      901 tcttaaacaa gcggtggagc aagagaaaat aagtcacgct tatctttttt ctggtcctcg
      961 tggaacggga aaaaccagtg ttgctaaaat ctttgccaag gctatgaact gtcccaatca
     1021 agtgggtggc gaaccttgca ataactgcta tatttgtcaa gcagtgacgg acggtagttt
     1081 agaagatgtc attgaaatgg atgcagcttc taataatggg gtagatgaaa ttcgcgaaat
     1141 tcgtgataaa tctacctatg cgcctagcct tgctcgttat aaggtttata tcatagatga
     1201 ggttcacatg ctgtctacag gggcttttaa tgccctccta aagacgctgg aagaaccaac
     1261 acagaatgta gtctttattt tggccactac tgaattgcac aagattcctg ctactattct
     1321 atcccgtgtg caacgttttg agtttaaatc aattaagaca caggatatta aggaacatat
     1381 tcactatatc ttagaaaaag aaaatatcag ttctgaacca gaggctgtgg aaatcattgc
     1441 caggcgggcg gaaggtggaa tgcgggacgc cttgtctatt ttggatcaag ccctgagttt
     1501 gacacaggga aatgagctga cgactgctat ctctgaagaa attactggca ccattagcct
     1561 atcagccttg gatgattatg tggcggcctt gtctcaacag gatgttccca aagctttgtc
     1621 ttgcttgaat cttctttttg acaatggtaa gagcatgact cgttttgtga ccgatctttt
     1681 gcactattta agagacttgt taattgttca aacaggggga gaaaatactc atcatagttc
     1741 agtctttgta gaaaatttgg cacttcctca aaaaaatctg tttgaaatga ttcgcttagc
     1801 aacagtgagt ttagcagata ttaagtctag tttgcagccc aagatttatg ctgaaatgat
     1861 gaccgtccgt ttggcggaaa tcaagcccga accagctcta tcaggagcgg ttgaaaatga
     1921 aattgctacg ctgagacagg aagttgcccg tctcaaacaa gagctttcta atgtaggtgc
     1981 ggttcctaaa caagttgcac cagctcctag tcgaccagct acgggcaaaa cagtctatcg
     2041 tgtcgatcgc aataaagtgc aatctatctt acaagaggcc gtcgaaaatc ctgatttagc
     2101 acgtcaaaat ctaattcgtt tgcagaatgc ctggggagag gtaattgaaa gtctaggtgg
     2161 gccggacaag gctctgctag ttggttctca accggttgct gccaatgaac accatgctat
     2221 tcttgctttt gagtctaact tcaatgctgg tcaaactatg aaacgagaca atctcaatac
     2281 catgtttggt aatatcctca gtcaggcggc aggtttttca cctgagattt tagctatttc
     2341 catggaggaa tggaaagaag ttcgcgcagc cttttcagcc aaagccaaat cttctcaaac
     2401 tgaaaaagaa gtagaagaaa gcctgattcc agaaggattt gaatttttgg ctgataaagt
     2461 gaaggtagag gaagactaaa gaaagatttc atgatacaat aagtttatga ataaacaaca
     2521 atttattatt atggcgctgt ttacagctgc tgagacctat tttttcaatg aagcctggat
     2581 gactggccgc tatattatgg cagccttttg ggcaatttta ctctttagaa atttccgagt
     2641 cagttatgtg atgggcaaaa tcgttgatgt catcgatcag cattttaata ggaaagacta
     2701 gccctcagct tccagacaaa atcaaagcct tttaggcttt tttttgttat actagaaaag
     2761 tatatttata gaatttttgc tctatttctg gggaaatcag acgtttttct agtaagtact
     2821 gtaaaagttt tgaaaaagaa aggaactatc atgtcagtat tagagatcaa agatcttcac
     2881 gttgagattg aaggaaaaga aattttaaaa ggggttaacc tgaccctgaa aacaggagaa
     2941 attgccgcta tcatgggacc aaatggtaca ggtaaatcga ctctttctgc cgctatcatg
     3001 ggaaatccaa actatgaagt aactaaaggt gaagttttgt ttgatggcgt aaacatcctt
     3061 gagttggaag tggatgagcg tgcgcgtatg ggacttttcc ttgctatgca atacccatca
     3121 gaaatccctg gaattaccaa tgctgagttt cttcgtgccg ctatgaatgc gggtaaagaa
     3181 gatgatgaga agatttcagt tcgtgagttt attactaagc tagatgaaaa aatggaattg
     3241 cttaacatga aagaagaaat ggcagagcgt tacctcaacg aaggcttctc tggtggtgag
     3301 aaaaaacgca atgaaattct tcaacttttg atgttggagc caacatttgc tcttttggac
     3361 gagattgact caggtcttga tattgacgct cttaaagttg tgtctaaagg tgtcaatgcc
     3421 atgcgtggtg aaggttttgg tgctatgatc attactcact accaacgtct tttgaactat
     3481 atcacacctg atgtggtaca cgtgatgatg gaaggtcgtg ttgtcctttc tggtggtcca
     3541 gaattggctg cgcgtttgga acgtgaagga tacgcaaaat tagctgaaga acttggctac
     3601 gactacaagg aagaattgta attccctcgt atcttttagg agaagtaaat gactagagaa
     3661 aatattaaac ttttttcaga aatgcacgct gaaccaagct ggttggctga tctccgtcaa
     3721 aaagcttttg acaagattga gactttggaa ttaccagtta ttgagcgtgt caaattccac
     3781 cgttggaatc tgggtgatgg aacgattaca gaaaatgagc catcagcaaa tgttccagat
     3841 ttcacagctt tagatcatca cttgaagttg gtgcaagtag gaactcaaac tgttttcgaa
     3901 caaactccag ttgagttagc tgaacagggt gttgtcttca cagactttca ctcagcttta
     3961 gaagaaattc cagagctgat cgaagaattc ttcatgtcat ctgttaagta tgatgatgac
     4021 aagttggcgg cttaccacac agcttacttt aacagtggtg ctgtactcta tattccagat
     4081 aatgtagaaa tcacagagcc aattgaagga attttctacc aagatagcga tagcaatgtg
     4141 ccgtttaaca agcatattat gattatcgct ggtaaaaatt ctaagattaa ttatctggag
     4201 cgtttagagt cacgcggtga aggaagtgcc aaagcaactg ctaatatcac agtagaagtg
     4261 attgcacgtt caggtgcgca agtcaagttt gctgctatcg accgtctagg tgaaaacgtc
     4321 actgcctaca ttagccgtcg tggtaaatta ggcaacgatg caagtattga ctgggctatc
     4381 ggtgtcatga acgaaggaaa tgtcgttgct gattttgata gtgacttgat tggtaatggt
     4441 agccatgctg acctcaaggt tgtagctctt tcaagtggtc gtcaggtaca agggattgat
     4501 actcgtgtaa ctaactatgg ctgcaactct atcggaaata tcctacaaca tggggttatc
     4561 cttgaaaaag caactttgac tttcaatggt atcggccaca tcatcaaggg tgctaaggga
     4621 gcagatgccc aacaagagag ccgtgttctc atgctttcag accaagcgcg ttcagatgct
     4681 aacccaattc ttttgattga tgaaaatgac gtcactgcag gacatgcagc ctctattggt
     4741 caggtagatc cagaagatat gtactacctc atgagtcgtg gattggataa ggcaactgca
     4801 gagcgtttgg ttgttcgtgg tttccttgga tctgttatcg tggagattcc agtcaaggaa
     4861 gttcgtgatg aaatgattgc aactatcgaa gagaaattgt caaaacgcta aggggaagcc
     4921 tatgttagat gtagaagcga ttcgcaagga ttttccaatt ttagatcaga ttgtcaatga
     4981 tgaacctctg gtctatctgg acaatgctgc gacgacacaa aaaccactag tagttctgaa
     5041 agctattaac agttactatg agcaggacaa tgccaatgtt caccgtggtg tccatacctt
     5101 agcggaacga gcgacagctt cttatgaagc tgctcgtgaa accattcgta agtttattaa
     5161 tgcaggctct acaaaggaag ttctctttac cagaggaacg acaaccagcc ttaactgggt
     5221 ggcacgcttt gctgaggaaa ttctcactga gggagaccag gtcttgattt cagtaatgga
     5281 acaccattct aatatcattc catggcagga agcttgtcga aagactggag cagagcttgt
     5341 ctatgtctat cttaaagacg gtgccttgga tatggaggat ttgcgagcta aattgactga
     5401 taaggttaaa tttgtttccc tagctcatgc ctccaatgtt cttggtgtgg tcaatccgat
     5461 caaggaaatc actcaattag cccaccaagt tggggcaatc atggtagtgg atggtgctca
     5521 atctacacct catatgaaga ttgatgtcca ggacttggat ctggactttt tcgccttttc
     5581 gggtcacaag atggctggtc cgactggtat cggtgtcctt tacggcaaag aaaagtatct
     5641 tgagcaaatg tctccagtag aatttggcgg cgagatgatt gattttgtct acgagcaatc
     5701 tgctagttgg aaggaattgc ctttgaaatt tgaggctgga acgccaaata tggcaggagc
     5761 tattggactt gcgactgcag ttgattatct ggaaaagatt ggtatggatg ccgttgaagc
     5821 tcatgaacag gaattgattg cgtacgtcta tccaaaactg caggcaattg agggattgac
     5881 catttacggt tctcaggatt tggctcaacg ttcgggtgtt attgccttta acctaggtga
     5941 tctccatcct cacgatcttg cgacggctct ggattatgaa ggagtggctg ttcgtgctgg
     6001 tcaccattgt gcgcaaccct tgcttcagta tttggaagtc ccagcaacag ctcgtgcaag
     6061 tttttatatc tataatacca aggcagattg cgacaaacta gtcgatgccc tacaaaagac
     6121 aaaggagttt ttcaatggca ctttctaaac tagatagcct ttatatggca gtggtagcag
     6181 accattcgaa aaatccacat caccaaggga agttagaaga cgctgagcaa atcagtctca
     6241 acaatccgac ttgtggagat gtcatcaatc tctctgtcaa gtttgatgca gaggaccgtt
     6301 tggaagatat tgcttttcta aattcaggat gcacgatttc aactgcttct gctagtatga
     6361 tgacagatgc cgttttagga aaaaccaaac aagaaatttt agaactggcg actatttttt
     6421 ctgaaatggt tcaagggcaa aaagatgagc gtcaagacca acttggagac gcggcattct
     6481 tgtcaggtgt tgccaaattc cctcaaagaa tcaagtgtgc aaccctagct tggaatgccc
     6541 ttaagaaaac aattgaaaat caagaaaaac agtaagacaa gtttcttttg tcttatgaat
     6601 tattagaaat gaagaaagaa aggatattat ggctgaagaa agagtagaac caaaaccaat
     6661 tgaccttggt gaatataaat ttggtttcca tgacgatgta gagcctgtct tatcgacagg
     6721 aaaaggactc aacgaaggtg ttattcgtga attatctgct gctaagggtg agcctgagtg
     6781 gatgttggag ttccgtttga agtcttatga aaccttcaaa aaaatgccca tgcaaacttg
     6841 gggagcagac ttgtcagaga ttgactttgg tgacttaatc tactaccaaa aaccatctga
     6901 caaaccagcc cgttcttggg atgatgtacc tgaaaagatt aaagaaacct ttgaacgtat
     6961 cgggattcca gaagctgaac gtgcttattt agcaggggct tctgcccagt acgagtcaga
     7021 agtggtttac cacaacatga aggaagagtt ccaaaaatta ggtattatct ttacagatac
     7081 agattccgca ctcaaggaat acccagactt atttaatcaa tactttgcga agttggtacc
     7141 gccgacagat aacaagttgg cagccctcaa ctcagcagta tggtcgggtg gaacttttat
     7201 ctacgtgcca aaaggtgtca aggtagatat tccacttcaa acttatttcc gtatcaataa
     7261 cgaaaatata ggtcagttcg aacgtacctt gattatcgtt gatgagggag caagcgtcca
     7321 ctacgtagaa ggatgtacag caccaacata ttcaagcaat agcttacacg ctgccattgt
     7381 agaaattttt gctttggacg gagcttatat gcgttataca actatccaaa actggtctga
     7441 taacgtctat aacttggtaa caaagcgtgc taaggctcaa aaggatgcca ctgttgagtg
     7501 gattgatgga aacttgggtg ccaaaacgac tatgaaatat ccatctgttt accttgatgg
     7561 agaaggagcg cgtggtacca tgctctctat cgcctttgct aatgcagggc aacaccaaga
     7621 cacgggtgct aagatgattc acaatgctcc acataccagc tcgtctattg tgtctaaatc
     7681 catcgctaaa ggtggaggaa aggttgacta ccgtggacaa gtcaccttta acaagaactc
     7741 taagaaatct gtttcccaca ttgaatgtga taccattatc atggatgact tgtcagcatc
     7801 agatactatt ccatttaatg aaattcacaa ctcgcaagtg gctttggaac acgaagccaa
     7861 agtatctaag atttcagaag agcaattgta ttatctcatg agccgtggat tgtcagaatc
     7921 tgaggcaact gaaatgattg tcatgggatt tgtagaaccc tttacaaaag aacttccaat
     7981 ggaatacgca gttgagctga accgcttgat tagctatgaa atggagggat cagttggata
     8041 aaatttgatt ttatactctt cgaaaatctc ttcaaaccac gtcagcatcg ccttaccgta
     8101 tgtatggtta ctgacttcgt cagtttcatc tacaacctca aaacagtgtt ttgagcaacc
     8161 tgaggctagc ttcctagttt gttctttgat tttgagtatt agatttactc aaaatcaagg
     8221 attttgaaga tgaacttgta tcaaaaaatc gcggtttaaa atcgcgattt tttataattt
     8281 ctcgttaaca aagcggacaa actgattcca ccaaactttt aagaagaagg ctttttcaat
     8341 tttcttgtct gctaccattt cgaaactagg gcgctctgtg gtgatgtaac cttgaccaat
     8401 caagtccttg tcttcataag tcaaatggcc aaccactgtt ccagcttcaa gtggtgctgg
     8461 gattgctttg gaatcaggtg tgaattgaac agattgggaa gattgattcc caacacgttc
     8521 gattagatag atatcctctg gagccactgc agttactgta tcttcttttc catcttgtac
     8581 aggggctttg ctatcttgat aggcatcgcc ttgttgaacg attttgcgaa gtgtaaatgt
     8641 agaagaaata taatccatta gggaagatgt agctgtaaat cgagcgtaag gattattgtc
     8701 ttgatgatct gtatttaaaa caactgtgat gactctcatg cctttttcga cagtagtacc
     8761 aacaaaagac tctccagcct tatctgttgt tcctgttttt agcccatcaa aaccaccacg
     8821 gtaagcaggc ataccttcta acatgtagtt ggttgaagtg attgtcatcc cagcaaaagt
     8881 agaagaaggt tttttggtga tttctaagac ttgtgggtat tttttgatga ggttgcgagc
     8941 aacgatagcg acatcataag cactaagctt attttcctca tcttttttag aacctgggta
     9001 aatgttatcc cctagagttt cattgttaag acctgtcgta ttgacaacag tggcatcctg
     9061 aattccccat tccaagagtt ttgcccgcat catatcgacg aaatcttttt ctgagccagc
     9121 aattttctca gctagggcaa tagcggcgct gttggcacta gataccagag ttgcttcaag
     9181 caactcttcg acagtataat tacgggcctc cataggaata ttactggctt cagaatttgt
     9241 cgtcaattga taaggataat cagaaatatc tacaggagtg gagagggtaa tacttccgtt
     9301 ttccaaagct tcatagacca gataaacagt aatcaatttt gttatggaag caatttcgac
     9361 aggttgcgtt gcatccttct catagagaat tttaccagta tttgcctcaa cagcaatcgc
     9421 atgtttagcg gcaatggtaa aatcttgagc aacagcagta gaagcacccc ctaaaagaga
     9481 gacagttaac aaagttaaaa atattttttt catagtagtc ttattctatc ataaagaaaa
     9541 aaaatattct tgctttaata attcatctgt taagcttttt gaaaatatgg taaaataaag
     9601 tgagggaggt aactcatgtt tcgtagaaat aaattatttt tttggaccac agaaatttta
     9661 ctcttaacca tcatctttta cctatggaga cagatggggt ctttgattaa cccttttgtt
     9721 agcgtgctta atacaattat gattccattt ttattaggag gctttcttta ttatttgaca
     9781 aaccctattg ttactttctt aaataaagtc tgtaaactca atcgtttgct tggtatttta
     9841 attaccttgt gtactttggt ctggggaatg gtcataggtg ttgtctatct cttacctatt
     9901 ttgattaatc agttatctag tttgattata tctagtcaaa ctatttatag tcgagtacaa
     9961 gacttaatca tagacttatc taattatcct gcgctccaga atttggatgt agaagctaca
    10021 attcagcagt taaacttatc ctatgtggat attcttcaaa atatcctaaa tagcgtatca
    10081 aatagtgtgg ggagcgtctt gtcagctctt atcagtactg ttttgatttt gattatgact
    10141 ccagtttttt tggtttattt cttattagat ggacataaat tcttgcccat gcttgaaaga
    10201 acgattctaa agagggatcg cttgcatatt gcaggcttat taaagaattt aaatgcgacg
    10261 attgctcgct atattagtgg agtttcgatt gacgcaatca ttataggttg tttggcttat
    10321 attggctata gtattattgg tttaaaatat gctttagttt ttgccatttt ttctggtgta
    10381 gccaatttaa ttccttatgt ggggccaagt attggtttga ttcctatgat catcgcaaat
    10441 atattcactg atccccatag actgctgatt gcagtgattt atatgcttgt tgttcagcag
    10501 gtagatggca atatcttata tcctcgaatc gtaggaagtg ttatgaaggt tcatccaatc
    10561 acgattttag ttttactttt gttgtcaagc aatatctatg gtgtagttgg aatgattgtc
    10621 gcagtgccaa cctattctat cttgaaagaa atttctaagt tcttatcccg tttgtatgaa
    10681 aatcataaaa taatgaaaga acgagaaaga gaattagcta agtaaaagtc aggagaaccc
    10741 tgatttttct ttactggaag tggcctttag attagaagac tgaaaataag ttaaagtctt
    10801 aaactaattt tcacagctaa gaatagtaga agttaatctg ataaaaatcg aaaaaaccag
    10861 tggaattctg tgtcagggta agttccactg gttttcatag tctattaaag ttcgaatgaa
    10921 acctatttat agtagattga aactagaata gtacacctct aattctaaaa cattgttaga
    10981 aatcgatttg actgtcctta tctattcgtt ctattcttat ttcattttac tatattttgg
    11041 tgcaataagt gaaaagtaga ccgaataata taaggattga ttttatagtt tttaaactca
    11101 aatgaattga aataaagaga gtacgaaaat tctcatctga aagtatttta gaataattct
    11161 cttcgtgaat ttcttcaaaa cagatagctt catcttaggt atgtgatttc tttttgcatt
    11221 tttgagttag ataaggtata atggttttat tgtcttttgg ggtcgttacg gattcgacag
    11281 gcattatgag gcatattttg cgactcgtgt ggcgacgtaa acgctcagtt aaatataact
    11341 gcaaaaaata acacttctta cgctctagct gcctaaaaac cagcaggcgt gacccgattt
    11401 ggattgctcg tgttcaatga caggtcttat tattagcgag atacgattaa gccttgtcta
    11461 gcggcttgat aagagattga tagactcgca gtttctagac ttgagttatg tgtcgagggg
    11521 ctgttaaaat aatacataac ctatggttgt agacaaatat gttggcaggt gtttggacgt
    11581 gggttcgact cccaccggct ccattattcc tttgcattct tttgcattcc ttggtaaaac
    11641 gttgttaaat caacgttttt tatttttatc tttggtattc ctttgtattc ttttgctaaa
    11701 aagggagtca caaacagacc ctattttaaa aaaaggatag aaaaaaggat acaacatttg
    11761 tcgcatccta aaaataatct tttttcgacg gaagacatgg gattcgaacc cacgcacgct
    11821 attacacgcc taccgcgttt ccaacacggc ctcttaagcc tcttgagtaa tcttccaata
    11881 cttactcaaa tagtctacca taaaggctct tatcttgcaa taaaaattct agaaataaga
    11941 aaaatgatag attttgaaag aaaatgataa aaaatgcttg acttcgaaag aaagtatgat
    12001 agaatgaata gtgtaaacga taacaggagg tgattcagtg ttaaaaacag aacgtaaaca
    12061 actaatttta gaggagttaa atcagcatca tgtagtttct ctagaaaaat tagttagttt
    12121 gctagaaacg tcagaatcaa cggttcgaag agacttggat gagttggaag cggaaaacaa
    12181 gcttcgtcgt gtgcatggtg gagcagaact cccccactcc ttacaggaag aagaaaccat
    12241 tcaagaaaaa tctgtcaaaa accttcaaga aaagaagttg ctggctcaga aagcagcctc
    12301 tctcattaaa gaaaaagatg tcatctttat cgatgctgga acaacaactg cttttttgat
    12361 tcatgaattg gtcaataaga atgttacagt tgtgaccaac tccattcacc atgccgctca
    12421 gttggttgaa aagcagattc caactgtcat ggttggagga aacgtcaaga cggcgacaga
    12481 tgctagtatc gggggcgttg ctcttaacca gattaaccaa ttgcactttg accgtgcctt
    12541 tatcggaatg aatggtgttg acgatggcta ttatacgact cctgatatgg aggagggagc
    12601 tgtgaaaaga gctattttgg agaatgccaa gcagacctac gtcttggtgg attcgtcaaa
    12661 aattggacaa acttgctttg ccaaggtagc accactcaaa cgcgctatcg ttatcactag
    12721 tcaagggcat gagctcttgc aggttattaa ggagaaaacg gaggtaatag aagtatgatt
    12781 tatacagtca cactcaatcc atccattgac tatatcgttc gtttggacca agtcaaagtt
    12841 ggtagtgtaa atcgtatgga cagtgatgat aagtttgctg gtgggaaagg aatcaatgtc
    12901 agccgtgtct tgaaacgttt gaatatacca aatacagcga cgggatttat cggtggcttt
    12961 actggtaaat ttatcacaga tactttagca gaggaagaaa tcgagacacg ttttgtccag
    13021 gtggcagaag atactcgtat caatgttaaa atcaaagcag accaagaaac agaaatcaac
    13081 ggaacgggtc caactgttga accggttcag ctagaagaat tgaaagctat tttatctagt
    13141 ctgacagcag aagatacagt tgtctttgca ggttcaagtg ctaaaaatct aggcaatgtt
    13201 atctataagg atttgatttc cttgacgcgc cagactggtg cgcaagtggt ctgtgacttt
    13261 gaaggacaga ccttaattga tagtttggac taccagcctc ttcttgtaaa accaaacaat
    13321 catgaacttg gagcgatttt tggggttaaa ctcgaaagtt tagatgaaat tgagaaatac
    13381 gctcgtgagt tactggctaa gggtgctcaa aatgttatta tctctatggc tggtgatggc
    13441 gcccttcttg tcacatctga gggagcttac ttcgctaaac caatcaaagg aacagtcaaa
    13501 aattcagttg gagctggtga ttctatggtt gctggattca caggtgaatt tgtcaaatca
    13561 aaagacgcag tagaagcctt caaatgggga gtggcttgcg gaacggcaac taccttctca
    13621 gatgacttgg caacggcgga atttattaaa gaaacatatg gaaaagttga ggtagaaaaa
    13681 agatgaaaat tcaagaccta ttgagaaaag atgtcatgtt gctagatttg caggcaactg
    13741 aaaaaacagc tgtcatcgac gagatgatta aaaatttgac agagcacggt tatgtaacag
    13801 attttgaaac atttaaagaa ggaattttgg cgcgtgaagc tttgacttct actggtttgg
    13861 gtgatggaat cgcaatgcct cacagcaaaa acgctgctgt caaagaagcg acagttctat
    13921 ttgctaagtc aaataagggt gttgactacg agagcttgga tggacaagca actgacctct
    13981 tcttcatgat tgcagctcca gaaggtgcca atgatactca cttggcagcc ttggcagaat
    14041 tgtctcaata cttgatgaaa gacggttttg cagacaaact tcgtcaagca acatctgcag
    14101 accaagttat cgaacttttt gaccaagctt cagaaaaaac tgaggaactt gttcaagcac
    14161 ctgctaatga ctctggtgac tttatcgtag ctgttacagc ttgtacaaca ggtattgccc
    14221 acacttacat ggcccaagaa gcccttcaaa aagtagctgc tgaaatgggg gttggtatca
    14281 aggtcgaaac caacggtgct agcggtgttg gaaatcaact aactgcagaa gatatccgta
    14341 aggctaaagc tattatcatt gcagcagaca aggccgttga aatggatcga tttgatggaa
    14401 aaccattgat caatcgtcca gttgctgacg gtatccgtaa gacagaagag ctaattaact
    14461 tggctctttc aggagatact gaagtctacc gtgccgctaa tggtgccaaa gctgcaacag
    14521 cctctaacga aaaacaaagc cttggtggtg ccttgtacaa acacttgatg agtggtgtat
    14581 ctcaaatgtt accattcgtt atcggtggtg gtatcatgat tgcccttgcc ttcttgattg
    14641 acggtgcttt gggtgttcca aatgaaaacc ttggcaatct tggttcttac catgagttag
    14701 cttctatgtt catgaaaatt ggtggagctg cctttggttt gatgcttcca gtctttgcgg
    14761 gttatgttgc ctactctatt gctgaaaaac cgggtttggt agcaggtttc gtggctggtg
    14821 ctattgccaa agaaggtttt gcctttggta aaattcctta tgccgcaggt ggtgaagcaa
    14881 cttcaactct tgcaggtgtc tcatctggtt tcctaggtgc ccttgttggt ggatttatcg
    14941 caggtgcctt ggttcttgcc atcaagaaat acgttaaagt tcctcgttca ctcgaaggtg
    15001 ctaaatcaat ccttctattg ccacttcttg gaacaatctt gacaggattt gttatgctag
    15061 ctgtgaatat cccaatggct gcaatcaaca ctgctatgaa tgacttccta ggcggtcttg
    15121 gaggaggttc agctgtcctt cttggtatcg tccttggtgg aatgatggct gttgacatgg
    15181 gtggaccagt taataaagca gcttatgtct ttggtacagg tacgcttgca gcaactgttt
    15241 cttcaggtgg ttctgtagcc atggcagcag ttatggctgg aggaatggtg ccaccacttg
    15301 caatctttgt cgcaactctt cttttcaaag ataaatttac taaggaagaa cgtaactctg
    15361 gtttgacaaa catcatcatg ggcttgtcat ttatcactga gggagcgatt ccatttggtg
    15421 ccgctgaccc agctcgtgcg attccaagct tcatccttgg ttcagcagta gcaggtggac
    15481 tcgttggtct tactggtatc aaactcatgg cgccacacgg aggaatcttc gttatcgccc
    15541 ttacttcaaa tgctctcctt tacctcgttt ctgtcttggt aggagcaatc gtaagtggtg
    15601 tggtttatgg ttacctacgc aaaccacaag cataaaaaat agaaaaatga aaagattgga
    15661 ccgtttggtg cagtcttttt ctcttcccga aatgcctgtg aaatatggta taatagaaga
    15721 atggcaaaca agaatacaag tacaacaaga cggagaccgt ctaaagcaga actggaaaga
    15781 aaagaagcga ttcaacgaat gttgatttcg ttaggaattg cgattttatt gattttcgca
    15841 gccttcaaat taggggctgc aggtataacc ctttataatt taattcgctt gctagtgggt
    15901 agcctagctt atctggcgat attcggccta ttaatctatc tcttcttttt caagtggata
    15961 cgaaaacagg aaggactctt atctggcttt ttcaccatat ttgctggctt actcttgatt
    16021 tttgaggcct acttggtttg gaaatatggt ttggacaagt ccgttctaaa agggaccatg
    16081 gctcaggttg tgacagatct gactggtttt cgaacgacta gctttgctgg agggggcttg
    16141 atcggggtcg ctctttatat tccaacagcc tttctctttt caaatatcgg aacttacttt
    16201 attggttcta tcttgatttt agtgggttct ctcctagtca gcccttggtc tgtttacgat
    16261 attgctgaat ttttcagtag aggctttgcc aaatggtggg aagggcacga gcgtcgaaaa
    16321 gaggaacgct ttgtcaaaca agaagaaaaa gctcgccaaa aggctgagaa agaggctaga
    16381 ttagaacaag aagagactga aaaagcctta ctcgatttgc ctcctgttga tatggaaacg
    16441 ggtgaaattc tgacagagga agctgttcaa aatcttccac ctattccaga agaaaagtgg
    16501 gtggaaccag aaatcatcct gcctcaagct gaacttaaat tccctgaaca ggaagatgac
    16561 tcagatgacg aagatgttca ggtcgatttt tcagccaaag aagcccttga atacaaactt
    16621 ccaagcttac aactctttgc accagataaa ccaaaagatc agtctaaaga gaagaaaatt
    16681 gtcagagaaa atatcaaaat cttagaagca acctttgcta gctttggtat taaggtaaca
    16741 gttgaacggg ccgaaattgg gccatcagtg accaagtatg aagtcaagcc ggctgttggt
    16801 gtaagggtca accgcatttc caatctatca gatgacctcg ctctagcctt ggctgccaaa
    16861 gatgtccgga ttgaagcacc aatccctggg aaatccctaa tcggaattga agtgcccaac
    16921 tccgatattg ccactgtatc tttccgagaa ctatgggaac aatcgcaaac gaaagcagaa
    16981 aatttcttgg aaattccttt agggaaggct gttaatggaa ccgcaagagc ttttgacctt
    17041 tctaaaatgc cccacttgct agttgcaggt tcaacgggtt cagggaagtc agtagcagtt
    17101 aacggcatta ttgctagcat tctcatgaag gcgagaccag atcaagttaa atttatgatg
    17161 gtcgatccca agatggttga gttatctgtt tacaatgata ttccccacct cttgattcca
    17221 gtcgtgacca atccacgcaa agccagcaag gctctgcaaa aggttgtgga tgaaatggaa
    17281 aaccgttatg aactctttgc caaggtggga gttcggaata ttgcaggttt taatgccaag
    17341 gtagaagagt tcaattccca gtctgagtac aagcaaattc cgctaccatt cattgtcgtg
    17401 attgtggatg agttggctga cctcatgatg gtggccagca aggaagtgga agatgctatc
    17461 atccgtcttg ggcagaaggc gcgtgctgca ggtatccaca tgattcttgc aactcagcgt
    17521 ccatctgttg atgtcatctc tggtttgatt aaggccaatg ttccatctcg tgtagcattt
    17581 gcggtttcat caggaacaga ctcccgtacg attttggatg aaaatggagc agaaaaactt
    17641 cttggtcgag gagacatgct ctttaaaccg attgatgaaa atcatccagt tcgtctccaa
    17701 ggctccttta tctcggatga cgatgttgag cgcattgtga acttcatcaa ggctcaggca
    17761 gatgcagact acgatgagag ttttgatcca ggtgaggttt ctgaaaatga aggagaattt
    17821 tcggatggag atgctggtgg tgatccgctt tttgaagaag ctaagtcttt ggttatcgaa
    17881 acacagaaag ccagtgcgtc tatgattcag cgtcgtttat cagttggatt taaccgtgcg
    17941 acccgtctca tggaagaact ggagatagca ggtgtcatcg gtccagctga aggtaccaaa
    18001 cctcgaaaag tgttacaaca ataaaaaaat agcttctttc cgagtttgga gggaagctat
    18061 tttagtggct attgattact tttatactct tcgcaaatct cttcaaacca cgtcagcttc
    18121 gccttgccgt actcaagtac tgtctacggc tagcttccta gtttgctctt tgattttcat
    18181 tgagtattat tttctgaagt tggagtattg ggctgttttt cattttcagt ggcaggttta
    18241 cttgtagcag gtgtagtaga gtcctgagtt gctgttttct gatcttcttt tttctcttcc
    18301 ttgacgctag attttggcgt ttcttcttgt tgtgtttttt cttgagaagt gttattttcc
    18361 ttatttggag tagtattttc ctgaggggat tctttttgaa tttctttgac aaaggttgtc
    18421 gtctggcttg tcgtaggttc ttttttagta tttttattat tattatccaa ggcgttcatg
    18481 atcgtgatac ccgcggtaat caaaccaagg atactaccga tagtagtaat ggttttttga
    18541 aaaacagtaa agcctttttt gatatgttct gttttcggtt ttgaagttct acgatagtta
    18601 gacatacatt ctctcctttg attatgattt attatactct gtttctttaa aaaaatctta
    18661 aaaaaagaga aattgccctt tcttgtcgca ggctccgttt catgatacaa tagaaggagt
    18721 gattttagaa agcgtgagaa acatgattta ctttgataat tcggcgacga ccaggcccta
    18781 tccagaagcc cttgaaacct atatgcaggt ggcttcaaaa attttaggaa atccatctag
    18841 tctccatcgt ttgggagacc aggcaacacg aattttagat gcttcccgtc aacagattgc
    18901 agatttaatc ggtaagaaaa gtgatgaaat cttctttaca tctggtggga cagaagggga
    18961 taactggatc atcaagggag tagcatttga aaaagcccag tttggcaagc acatcatcgt
    19021 atcagccatt gaacatccag cagttaagga atcagccctc tggttgaaag ctcaaggttt
    19081 tgaagtggat tttgctccag ttgataacaa aggatttgtg gatgttgagg cgctagaaga
    19141 tttgataaga cctgatacga ccctcgtttc catcatggct gtgaacaatg aaatcggctc
    19201 tatccaacct attgaggcta tttcagaatt cttggcagac aagccaacta tttccttcca
    19261 cgttgatgcg gttcaggcgc ttgccaagat tccgactgaa aagtatctga cagaacgagt
    19321 ggatttcgcg actttctctg gtcacaagtt ccacggaatc cgtggtgttg gttttatcta
    19381 tatcaagtct ggcaagaaga ttacgcctct tttaacaggt ggtggtcagg aacgtgatta
    19441 ccgttcgaca actgaaaatg tggcaggaat tgcagcgacc gctaaggctc tccgtttatc
    19501 tatggaaaag ctagatatct ttaggagcaa gactgggcag atgaaggcag tgattcgtca
    19561 agcacttctg gactatccgg atatatttgt cttttcagat gaggaagact tcgcccctca
    19621 tattctgact tttggaatca agggtgttcg tggtgaggtc attgttcacg cctttgaaga
    19681 ctatgatatt ttcatctcaa cgacctcggc ttgttcgtcc aaggctggga aaccagccgg
    19741 aaccctgatt gctatgggag tggacaagga taaggctcag tcagctgtgc gtcttagtct
    19801 agaccttgaa aatgatatga gtcaggtcga gcagttcttg accaagctaa aattaattta
    19861 caatcaaact agaaaagtaa gataggagca ttcatgcagt attcagaaat tatgattcgc
    19921 tacggtgagc tgtcaaccaa gggcaaaaat cgtatgcgtt tcatcaataa acttcgcaat
    19981 aatatttcag acgttttgtc tatctatccc caagttaagg taacagcaga tcgcgaccgt
    20041 gcccacgctt acctcaatgg agcagattac acagcagtag cagaatcgct caaacaagta
    20101 tttggaattc aaaacttttc ccctgtttat aaggttgaaa aatctgtaga agttctgaag
    20161 tctgctgtcc aagagattat gcaggacatc tacaaggaag gcatgacctt taaaatctct
    20221 agcaagcgta gcgaccacac ctttgaattg gacagtcgtg aactcaacca aacgcttgga
    20281 ggggctgtat tcgaagccat tccaaacgta caagctcaaa tgaaaagtcc tgacatcaat
    20341 cttcaggtgg agatacgtga agaagcagcc tatctttctt atgaaaccgt tcgaggggca
    20401 ggtggattac cagtgggaac ttctggtaag ggcatgctca tgttgtcagg gggaatcgat
    20461 tcccctgtag ctggctatct agcgcttaaa cgaggggtag atatagaggc agttcacttt
    20521 gccagcccac cttacacgag tcctggtgct cttaaaaaag cccaagattt gacccgtaaa
    20581 ttaaccaaat ttgggggaaa tatccagttt atcgaagttc cttttacaga gattcaagag
    20641 gaaatcaaag ccaaagcgcc agaagcctac cttatgacct tgacgcgtcg ttttatgatg
    20701 cgtattacgg accgtattcg tgaggtacga aatggtttag ttatcatcaa tggggaaagt
    20761 ctaggtcaag tagccagcca aacccttgaa agtatgaagg ctatcaatgc tgttaccaac
    20821 actcccatca ttcgtcctgt ggttaccatg gacaagttgg aaatcattga catcgcccag
    20881 gaaatcgata cctttgacat ttcaatccaa ccgtttgaag actgttgtac tatttttgca
    20941 ccagatcgtc caaaaacaaa tcctaaaatt aagaatgcgg agcagtacga agcgcgtatg
    21001 gatgttgaag gcttggttga gcgagcagtg gctggaatca tgattactga aatcacacct
    21061 caagccgaaa aagatgaagt tgatgacttg attgacaatc tgctctaatt cagaaaatcc
    21121 aaaagaatag cgaaaatcag taaaaaaagt tagttttttc tctaaaaaca ggtaaaaaac
    21181 taactttttt tatttttatg atataatgat ataaaatttt gaatatagag agttttctga
    21241 caattaatca atcctacttt tatctaaaaa tgaaagaaca caaactcaaa gttccttata
    21301 caggtaagga gcgccgtgta cgtattcttc ttcctaaaga ttatgagaaa gatacagacc
    21361 gttcctatcc tgttgtatac tttcatgacg ggcaaaatgt ttttaatagc aaagagtctt
    21421 tcattggaca ttcatggaag attatcccag ctatcaaacg aaatccggat atcagtcgca
    21481 tgattgtcgt tgctattgac aatgatggta tggggcggat gaatgagtat gcggcttgga
    21541 agttccaaga atctcctatc ccagagcagc agtttggtgg taagggtgtg gagtatgctg
    21601 agtttgtcat ggaggtggtc aagcctttta tcaagcacaa aacaggttgg tttgatggca
    21661 tgatgacgac aggttgctct atgggagcct atcatgcact caatttcttc ctccagcatc
    21721 cagatgtctt taccaaagtg attgctctca gtggtgttta cgacgcacgt ttctttgtcg
    21781 gcgattacta caacgatgat gctatttacc aaaactcgcc agtagattat atttggaacc
    21841 aaaacgatgg ctggtttatt gaccgttacc gtcaggcaga gattgtgctg tgtacggggc
    21901 ttggagcctg ggaacaagat ggtttaccat ccttttacaa gctcaaagaa gcctttgacc
    21961 agaaacaaat tccagcctgg tttgctgaat ggggacatga tgtcgcccac gactgggaat
    22021 ggtggcgtaa acaaatgctt tatttcctcg gtaatctcta tttataaaag gagttaccta
    22081 tgaattacct tgttatttct ccctactatc cacaaaactt tcaacagttt accatcgaac
    22141 tagctaataa aggcatcaca gtcttgggaa ttggtcaaga gtcttacgag caattggatg
    22201 agcccttgcg caatagcttg accgagtatt ttcgtgttga taatcttgag aacatagatg
    22261 aagtcaaacg tgcagttgct tttctctttt ataaacatgg tccaattgac cgcatcgagt
    22321 ctcacaatga atactggctt gagctagacg caacactcag agaacaattc aatgtttttg
    22381 gtgccaaacc agaggatctc aaaaagacga aatataagtc tgaaatgaag aaacttttca
    22441 aaaaagcagg tgttcctgtg gtacctggag ctgttatcaa gacggaagca gatgttgatc
    22501 aagcagtgaa agaaatcggt cttccaatga ttgccaaacc tgataatgga gtgggagcag
    22561 ccgcaacctt taaacttgag acagaagacg atatcaatca cttcaagcaa gaatgggacc
    22621 attcaaccct ttatttcttt gaaaaatttg tcacttccag cgaaatctgt acctttgacg
    22681 ggctcgtgga caaggatgga aagattgtct tctcaacaac ctttgactac gcctatacac
    22741 cgcttgacct catgatttat aagatggaca attcttatta tgtgctcaag gatatggatc
    22801 ctaaactgcg caagtatggg gaagcaattg tcaaagaatt tggtatgaaa gaacggtttt
    22861 tccatattga gttcttccgt gagggggacg attatattac catcgagtac aataaccgcc
    22921 ctgcaggtgg ttttaccatt gatgtttata actttgctca ttccttggac ctttatcgtg
    22981 gctatgcagc tattgtcgca ggagaggagt tcccggcgtc agactttgaa actcagtatt
    23041 gtttggctac ttctcgccgt gcaaatgctc actatgttta ttcagaagag gatttgcttg
    23101 ccaaatatag ccagcagttc aaggttaaaa aagtcatgcc agctgccttc gcggaacttc
    23161 aaggagatta cctgtatatg ctgacaactc cgagtcgaca agaaatggat cagatgattg
    23221 ctgatttcgg acaacgtcaa gaataagaac tatcggatta aggaaattca ctcccttaat
    23281 ccttttgttt tgtctggtaa aaaataagag catcctaaca aggtagctat cataaaactt
    23341 gttcgataac tatttgaagc aggattaggt ggctagaaat tgaattttaa catttcaatt
    23401 tagtcatagt attgtgtttt cgtatcctta aatcagctaa aaggatccat gacgacacct
    23461 atacgatata gttttcagga taccaaacca gtctattaat actcaatgaa aatcaaagag
    23521 caaactagga agctagccgc aggttgctca aaacactgtt ttgaggttgt ggatagaact
    23581 gacagagtca gtatcatata cctacggcaa ggtgaagctg acgtagtttg aagagatttt
    23641 cgaagagtat aaatcttatt gatgaactgc ttgcagtctg agaaaaaatg agcttggata
    23701 ttatttccaa actcacttaa agtcaatttc aatccactag aacaagccta gtacagttcc
    23761 atcgctttca acatccatgt tgagagctgc tggacgtttt ggaagacctg gcatggtcat
    23821 aacatcacca gttaaggcaa cgatgaagcc tgcacctaat tttggtacca attcacgaat
    23881 ggtaatttca aagttttctg gtgctccaag cgcatttgga ttgtctgaga aactgtattg
    23941 agttttagcc atacagattg gcaatttgtc ccaaccgttt tgaacgattt gagcaatttg
    24001 tgtttgagct ttcttctcaa agttcacttt gctaccacga tagatttcag tgacaatttt
    24061 ttcaatcttt tcttggacag aaagggcatt atcgtacaaa cgtttatagt tagctggatt
    24121 ttcagcaatt gtcttaacaa ctgtttcggc aagtgctact ccaccttctg ctccatcagc
    24181 ccagacacta gccaattcaa ctggtacatc gattgaggca cagagttctt ttaagactgc
    24241 aatttcagct tctgtatcag atacaaattc gttaatagct acaactgctg gaataccgaa
    24301 cttacggata ttttcaacgt ggcgtttcaa gttagcaaaa cctgcacgaa ctgcctctac
    24361 attttcttca gtcagagcgt ctttagccac accaccattc atcttaaggg cacgaagggt
    24421 tgcgacaatg acaactgcat ctggagatgt tggcaagttt ggtgtcttga tatcaaggaa
    24481 tttctcagca ccaaggtccg caccaaaacc agcttcagta acagtgtaat cagccaagtg
    24541 aagggctgtt gtcgtcgcca aaacagagtt acagccatga gcgatattgg caaatggacc
    24601 accgtgtaca aaggcaggtg taccgtaaat tgtctgaacc aagtttggct taatagcatc
    24661 cttcaaaatc aaagctaagg caccctcaac ctgcaaatca cctacagaaa caggcgtacg
    24721 gtcatagcga taaccaataa cgatattcgc caaacgacgt ttcaagtcct cgatgtccgt
    24781 tgccaagcaa agaattgcca tgatttctga agcaactgta atatcaaaac catcctcacg
    24841 tggaataccg tttagaggac caccaagacc aacagtcaca tggcggagcg cacggtcgtt
    24901 caagtccaca acgcgtttcc agaggatacg acgttgatca atttccagct cattcccttg
    24961 gtgcaagtgg ttgtcaatca aggcagaaag ggcattgttg gcagttgtaa tagcatgcat
    25021 atctccagta aagtggaggt tgatgtcttc cattggcaga acttgtgcat acccaccacc
    25081 agcagcacca cccttgatcc ccatgactgg accaagagac ggttcgcgga tagcaatcat
    25141 ggttttcttg ccaatcttgt tcaaggcatc cgcaagacca atggtaagcg tcgactttcc
    25201 ttcacctgca ggtgttgggt tgatggcagt aaccaagatc aatttaccga ctggattgct
    25261 ctcaactgca cgaattttat caaagctgag tttagccttg tactttccgt acaactccaa
    25321 atcgtcataa gaaataccaa gtttctctac aacatcaaca attggcttca actcaatact
    25381 ctgtgcgatt tcaatatctg ttttcattca aaattcctct aacctcttat atgataattc
    25441 attatatcac aaaacaagat ttttaacatc ctaaaactct ctaaacgttc gtaaatatct
    25501 ctgtttttaa ggattttaga gtcctttctt aaattttata tggctttata gtttgaaact
    25561 atagtaaatc ttcgttttta ccaaaaattt atcactttca ttttacttac cgcttatttt
    25621 tgtgtacaat agtgctatga aaattttagt tacatcgggc ggtaccagtg aagctatcga
    25681 tagcgtccgc tctatcacta accattctac aggtcacttg gggaaaatta tcacagagac
    25741 tttgctttct gcagggcatg aagtttgttt aattacgaca aaacgagctc tgaagccaga
    25801 gcctcatcct aacctaagta ttcgagaaat taccaatacc aagaaccttc taatagaaat
    25861 gcaagaacgt gttcaggatt atcaggtctt gatccactca atggctgttt ctgactacac
    25921 tcctgtttat atgacagggc ttgaggaagt tcaggctagc tccaatctaa aagaattttt
    25981 aagcaagcag aatcatcagg ccaagatttc ttcaactgat gaggttcagg ttttgttcct
    26041 taaaaagaca cccaaaatca tatccctagt caaggaatgg aatcccacta ttcatctgat
    26101 tggtttcaaa ctgctggttg atgttaccga agatcatctg gttgacattg cacgaaaaag
    26161 tcttatcaag aatcaagcag atttaatcat cgcgaatgac ctgactcaaa tttcagcaga
    26221 tcagcaccga gctatatttg ttgagaaaaa tcagcttcaa acagtccaga ctaaagaaga
    26281 aattgcagaa ctcctccttg aaaaaattca agcctatcat tcttagaaag gaaaactatg
    26341 gcaaacattc tcttggctgt aacgggttca atcgcctctt ataagtcggc agatttagtc
    26401 agttctctaa aaaaacaagg ccatcaagtc actgtcttaa tgactcaggc tgctacagag
    26461 tttatccaac ctttgacact acaggtactc tcacagaatc ctgtccactt ggatgtcatg
    26521 aaggaaccct atcctgatca ggtcaatcat atcgaacttg gaaaaaaagc agatttattt
    26581 atcgtggtac ctgcaactgc taacactatt gcaaaactag ctcacggatt tgcggacaac
    26641 atggtaacca gtacagctct agccctacca agtcatattc ccaaactaat agctcctgct
    26701 atgaatacaa aaatgtatga ccatccagta actcagaata atctgaaaac attagaaacc
    26761 tacggctatc agctgattgc tcctaaggaa tccctactag cttgtggaga ccacggacga
    26821 ggagctttag ctgacctcac aattatttta gaaagaataa aggaaactat cgatgaaaaa
    26881 acgctctaat attgcaccca ttgctatctt ttttgctacc atgctcgtga tacactttct
    26941 gagctcactt atctttaacc tttttccatt tccaatcaaa ccgaccattg ttcatattcc
    27001 tgtcattatt gccagcatta tttatggtcc acgagttggg gttacacttg gatttttgat
    27061 gggattactt agcttgacgg ttaacacgat tacgattcta ccgacaagct acctcttctc
    27121 tcccttcgta ccaaacggaa acatctactc agctatcatt gccatcgtcc cacgtatttt
    27181 gattggttta actccttact tagtctataa actgatgaaa aacaagactg gtctgatttt
    27241 agctggagcc ctcggttcct tgacaaatac tatctttgtc cttggaggaa tcttcttcct
    27301 atttggaaat gtttataatg gaaatatcca acttcttctg gcaaccgtta tctcaacaaa
    27361 ttcaattgct gaattggtca tttctgcaat tctaacccta gccattgttc cacgactaca
    27421 aaccttgaaa aaataaaaac aatctccgct ttcaaacctg aaggtggaga ttttgctcta
    27481 tacagtttct ttttaatgaa atctttacta tagtaaaacg aaataagaac aggacaaatc
    27541 gatcaggaca gtcaaatcga tttctaacaa tgttttagaa gtagaggtgg actattctag
    27601 tttccatcta ctataacttt taactaaaaa atgatctata aacccaagta aattcttgct
    27661 ttattgtctg gtttattgta ccatactagt gtatatgcag ttaaaaagga gattcttgtg
    27721 aatacacgga aaaagacaca atttatgaca atgacagccc ttttaacggc tattgcgatt
    27781 ttgattccaa ttgttatgcc tttcaagatt gtcattccac ctgcttccta tactttgggg
    27841 agccacatcg ctatttttat agccatgttc ttgtcgccct tgatggcagt ttttgtcatc
    27901 ctagcctcta gttttggatt tttgatggct ggctatccca tggttatcgt ttttcgggct
    27961 ttttcccata tatcttttgg tgctttagga gctctttacc tacaaaaatt ccccgatacc
    28021 ctagataaac caaaatcttc ctgggttttc aattttgttt tggctgttgt tcatgccctt
    28081 gctgaagtat tagcctgtgt ccttttttac gcaacttcgg gtaccaatgt agaaaatatg
    28141 ttctatgttc tatttgtact agttggattt ggcacaatta tccatagtat ggtagactat
    28201 acattagcac tagctgtcta taaagtgctt cgaaaacgcc gttaaaagga aaagctatga
    28261 caaaagatcg caaacaagcc ctgctccaac tcttgaaaga agctcctaaa gcccttaatg
    28321 gccaaagttt ggcggaacat tttcatgtca cacgtcaggt cattgtgcag gacattgcaa
    28381 ttttgagagc cgatggcgct cctatcctat ccaccaatcg tggctacatc tataagcaaa
    28441 ttgataccaa tccttatgtt cacaaacttt tcaaagtgaa acatgaagtt gaagaaatcg
    28501 gtcaggaact ccttgctatt gtcgataatg gtgggcatgt ccagaatacc ttgattgacc
    28561 atcccgttta tggagaaatt gaaaccttgc ttaaactgtc ttgtcgcaga gatgtgcaac
    28621 attttctaga acaagtcgag cattctgact ttagacctct atctgaattg acagatggca
    28681 tccattacca cctagtcgaa gccgaaacac aacaagacct ccactatatc gaggaggcct
    28741 tggatcagct aggttattta gtaaaagact agaagatttt cttttcccaa tcgtcttctg
    28801 tacggcgagg atagaaaaac tctgacttgt cgggagcttc catcatttcc atcaatggta
    28861 gcatatcata ggccagattt aagtttggaa tctggtcttt ttgcacccaa gaaacttctc
    28921 cctcttctga agattgaagg gtaccagaga actcagtcgc cttataacaa atgacaatat
    28981 agcgcccacc tgtatctagt ggccaatttt taatgccgac aagttgagga ttttggatag
    29041 tcaaccctgt ttcttcgtag atttcacgaa tgacagactc cgcaaaagcc tcatcatttt
    29101 ctacatgacc tccaggaaag gcataaccag accagcgatt gttttcaggg gcgcgatact
    29161 gcatcaccac gcgctgagtt tcgaggtctt caatcagaca gatatttgtt aaaattgtta
    29221 attgggaacg ggacataaat ttactcgctt tctaatttat taaattttca gacttactac
    29281 cagagatatt atcttttact tttttaaaat tatttagaat agaacaccat tcgtttttcc
    29341 tttataggct ttccaagttc aagtattttt tcttgtccat caaaagtaaa acccattttt
    29401 tgatagaaag caatggcgcg cttgttatct ttcaataccc ataagaaaat ttcagaaaaa
    29461 tgattaagag cagtcaaagc tgctttcatt aacttttgtg cgataccttt tccataatag
    29521 tcttttaaaa catataaagc aataatttca ccagcttgaa tagtctcatc acgaaaattg
    29581 ccataactga taaaaccaac caccttcacg ccatccatcg caatcaatgt attttctgga
    29641 tacttttgac taaagaatcg acatctttct aatgtcattg tatcctgaaa ttccgcaggc
    29701 aaaaggtcat cataagactc tctccacgtt tgccagtgaa cgagggattt gccttctatc
    29761 tcttcaggag tttccattga tttgataata accgtcattt attttctccc agtcttctct
    29821 caaaatacca tatttaatac tatcaaaata tttaccttga taataacgaa cttttggaat
    29881 atgagcttct tttttcattc ttaatttttc agcaagtttc atcataccaa gatttcctga
    29941 ccaagttgtc aaaaccagat gctccaactc caagtaatcc tgaaacgtcc tatctatcca
    30001 ctgcaacata gcaactttcc caataccagt gttccagaat tttttatcat aaataccaat
    30061 tcccaattcc atccatcttg tttctttaca tacccaataa cgcgaaacag tcccaactag
    30121 tttatcatca acaaaaatac caaggcgatt tgagttgctt aaaatggatt ctgatttttg
    30181 tagttcgaat tctttaaaat ttgaaaaata ctgataatca tcataatagg gagcatcata
    30241 ctgtttccaa actggattaa gctgtgaata agccatttcc cataaagaca tcaaatcttc
    30301 tttctttaac tttcttaaat aaatcatact aattccccta atccaaggcc ttgacttcca
    30361 acatcatatc acggatctga gccgccaatt caaagtcaag cacttcgacg gcttcttgca
    30421 tttgtttttc tagtttcttg acgagttctt tgcgctcttg tttgttgagg ctattgatgt
    30481 cgacttcctt gtcctcttcc ttagcaactg ccttggtcac ggcaatcagg tcacggattt
    30541 ctttcttgat tgtctgtggt acgataccat gctcttcatt ataggccatc tgaattttcc
    30601 gacggcgggc agtttcatcg atagcacgtt gcatagactg ggtaaccgta tctgcataca
    30661 tgatgacatg accttcactg ttacgggcgg cacgcccaat ggtctggatg agtccacgct
    30721 cgttacgaag gaaaccttcc ttgtcagcat cgagaatggc aaccaagcta acctcaggaa
    30781 cgtcaatccc ttcacgaagg agattgattc caaccaagac atcaaagaca cccaagcgga
    30841 gatcacggat aatctccgtc cgttccaagg tcttgatatc cgagtgcatg tacttgacct
    30901 tgatgcccat ttccttgaaa tagtcggtca agtcctctgc cattttcttg gtcaaggtgg
    30961 tgataaaggt tcgttcattc ttttcaacac gggcattgat ttcacctaag agatcatcaa
    31021 tctgtcccat agtcggacgg acttccacct caggatccaa aagccctgtc ggtcgaatga
    31081 tttgctcaat caccgtctca gtctgttcat tttcatagtc accaggtgtc gctgatacgt
    31141 aaacaatctg atgcacatgg ctttcaaact cctcacgacg gagaggacga ttgtccaagg
    31201 cagacggtaa acggaaacca taatttacta gcatttcctt acgcgaacgg tctccattgt
    31261 acatgccttt tatttgtccc atagtcatat gactctcgtc aatcataatc aaaaaatcat
    31321 ctgggaaaaa gtcgagaagc gtataaggag gctctccttc gctccgtcca tccatgtggc
    31381 gagaataatt ttcaacccca tttgtatagc ccatctcgcg cagcatttcg atatcgtact
    31441 ctgtccgttg tttcaagcgc tgggcttcaa gcaatttacc ttccttctca aagacagcta
    31501 actgctcttc taattctgct tgaatcttgg caacggcaac ttccatatgg tcgtcattgg
    31561 tcacaaagtg agtagctggg aaaatcgcca aatgatccac ctctcccaat acctgacctg
    31621 tcagagcctc aacctcacgg atacggtcaa tttcgtctcc aaaaaattct actcgaaagg
    31681 catgttcatc tcgggaagct gggaaaatct ctaccacatc cccacgaacg cgaaatcttc
    31741 cgcgttggaa atcaatatca ttacgttcaa actgaatatc gaccaagtca ttcaagagtt
    31801 tatcacgaga aatctctaga ccaggacgga gactaacgac actatcagcg tattccttgg
    31861 gcgaacccaa accatagata caagagactg aggccacgac aataacatca ttacgctcca
    31921 aaagggctga ggtagctgag tggcgaagct tgtcaatctc gtcattgaca gaactatcct
    31981 tctcaatata ggtatcgcta gaagggacat aggcctctgg ctggtaataa tcatagtagg
    32041 atacgaaata ctcaactgca ttttcaggga aaaattcctt aaactcccca tagagctgac
    32101 cagccagagt tttattgtgg gcaataacca gagttggttt attgacttta gaaatgacct
    32161 gactcatagt ataggtcttc cctgttccag tcgcccccat cagaatctga gctttttctc
    32221 ccccctcaat gttatccacc aactgctcga tagcttgggg ttgatctcct gatggttgat
    32281 attttgatac tagtttaaat tgattatctg taatgtgatt gatcataaga atcctctctc
    32341 catgtgctat tcctatttta gcatacttat agtattttca cgaagaaaag gacggaccga
    32401 agtcacatcc tttcattttc tttctttaat ttattggcaa gatagacaat cagcagtagt
    32461 aataccagac ttgtcataat ggaaccttct atgccaaaag aaccacctga tagccaatct
    32521 tgattgcctt gtggtaaaaa ggtcatcaga gacgttcctg acggttgacc actaactaaa
    32581 atcccaaaga gattaccctg agcaaaattc caagcaccat gaatacctgc aacaccccaa
    32641 actgtatcag ttttgagaag gtaaagagcc atggcaactc cgaataaaaa gagatttact
    32701 agagatagag gggtgaggcc agaattgccc atatgaagca gggtaaagaa caggctagat
    32761 ataagaatag ctagttttag attggttctt gaggccaatt gaggaagtag ccaagcacgg
    32821 gccaccactt cttctgctgt cccctgtaaa atccaaaatg ggatagtaaa gacgacaaag
    32881 gcaagagaat aaggattcaa gtgaatggat tccaaacgat attgacctaa gaccaccaaa
    32941 cctaacaagg tcagaagaaa aagtgccaga cctagactaa atcctttcag aagattgctg
    33001 aggaaattct ctctataaaa tcccaaggtt cgaataggtc ttttctcaac ttttcttgtc
    33061 catctgaaca cagtgttaag aataaaacca aaggccagta atcctaaaat taaacgaaag
    33121 tcacttgttt tatccgtcaa gctctgatgc aaggtctgca agtagcttgc aagattttga
    33181 ccagcctctc caaaatttct agcaagtccg atgagagcta acaagccaat accatacaaa
    33241 gtaaaagcca caaactctcc tatgaagaca aaaacaaagg ctaacaaggg gcttgtgtaa
    33301 atgtttaaac tcatttttga agcttggaag tctttaaata ttcttttgtt cttcatatcc
    33361 ccgtcctctt ttcttctata tatactatta ttatagcact ttttaatagc aattcaagga
    33421 aaggaaaaaa tcgatgttat attttctgac acgaaaaata taaaaatttg ccttttttta
    33481 ctttttctga tataatgaga gaatattcgg aaaaggagac taaaaatgaa gaaaaaattt
    33541 ctagcatttt tgctaatttt attcccaatt ttctcattag gtattgccaa agcagaaacg
    33601 attaagattg tttctgatac cgcctatgca ccttttgagt ttaaagattc agatcaaact
    33661 tataaaggaa ttgatgttga cattattaac aaagtcgctg agattaaagg ctggaacatt
    33721 cagatgtcct atcctggatt tgacgcagca gtcaatgcgg ttcaagctgg gcaagccgac
    33781 gctatcatgg cagggatgac aaagactaaa gaacgtgaaa aagtcttcac catgtctgat
    33841 acttactatg atacaaaagt tgtcattgct actacaaagt cacacaaaat tagcaagtac
    33901 gaccaattaa ctggcaaaac cgttggtgtt aaaaacggaa ctgccgctca acgtttcctt
    33961 gaaacaatca aagataaata cggctttact attaaaacat ttgacactgg tgatttaatg
    34021 aacaacagct tgagtgctgg tgccatcgat gccatgatgg atgacaaacc tgttatcgaa
    34081 tatgccatta accaaggtca agacctccat attgaaatgg atggtgaagc tgtaggaagt
    34141 tttgctttcg gtgtgaaaaa aggaagtaaa tacgagcacc tggttactga atttaaccaa
    34201 gccttgtctg aaatgaaaaa agatggtagt cttgataaaa ttatcaagaa atggactgct
    34261 tcatcatctt cagcagtgcc aactacaact actctcgcag gattaaaagc cattcctgtt
    34321 aaggctaaat atatcattgc cagcgattct tcttttgcac cttttgtttt ccaaaattca
    34381 agcaaccaat acactggtat tgatatggaa ttgattaagg caatcgctaa agaccaaggt
    34441 tttgaaattg aaatcaccaa ccctggtttt gatgctgcta tcagtgctgt ccaagctggt
    34501 caagccgatg gtatcatcgc tggtatgtct gtcacagatg ctcgtaaggc aacttttgac
    34561 ttctcagaat catactacac tgctaatacc attcttggtg tcaaagaatc aagcaatatt
    34621 gcttcttatg aagatctaaa aggaaagaca gtcggtgtta aaaacggaac tgcttctcaa
    34681 accttcctaa cagaaaatca aagcaaatac ggctacaaaa tcaaaacctt tgctgatggt
    34741 tcttcaatgt atgacagttt aaatactggt gccattgatg ccgttatgga tgatgaacct
    34801 gttctcaaat attctatcag ccaaggtcaa aaattgaaaa ctccaatctc tggaactcca
    34861 atcggtgaaa cagcctttgc cgttaaaaaa ggagcaaatc cagaactgat tgaaatgttc
    34921 aacaacggac ttgcaaacct taaagcaaac ggtgaattcc aaaagattct tgacaaatac
    34981 ctagctagcg aatcttcaac tgcttcaaca agtactgttg acgaaacaac gctctggggc
    35041 ttgcttcaaa acaattacaa acaactcctt agcggtcttg gtatcactct tgctctagct
    35101 cttatctcat ttgctattgc cattgtcatc ggaattatct tcggtatgtt tagcgttagc
    35161 ccatacaaat ctcttcgcgt catctctgag attttcgttg acgttattcg tggtattcca
    35221 ttgatgattc ttgcagcctt catcttctgg ggaattccaa acttcatcga gtctatcaca
    35281 ggccaacaaa gcccaattaa cgactttgta gctggaacaa ttgccctctc actcaatgcg
    35341 gctgcttata tcgctgaaat cgttcgtggt ggtattcagg ccgttccagt tggccaaatg
    35401 gaagccagcc gaagcttggg tatctcttat ggaaaaacca tgcgtaagat tatcttgcca
    35461 caagcaacta aattgatgtt gccaaacttt gtcaaccaat tcgttatcgc tcttaaagat
    35521 acaactatcg tatctgctat cggtttggtt gaactcttcc aaactggtaa gattatcatt
    35581 gctcgtaact accaaagttt caagatgtat gcaatccttg ctatcttcta tcttgtaatt
    35641 atcacacttt tgactagact agcgaaacgc ttagaaaaga ggattcgtta atggcaaaat
    35701 taaaaattga tgtaaatgat ttacacaagc actatggaaa aaatgaagtc ctaaaaggaa
    35761 ttacgactaa gttctatgaa ggagatgttg tttgtatcat cggtccttca ggttctggta
    35821 agtcaacttt cctccgtagc ctcaatcttt tagaagaagt cactagcggt cacatcactg
    35881 tgaacggcta tgatttaact gaaaaaacaa ccaatgttga ccacgtccgt gaaaatatcg
    35941 gcatggtatt ccaacacttc aacctcttcc ctcatatgtc tgtattggac aacatcacct
    36001 ttgctcctat tgagcacaag ttgatgacta aggaagaagc tgaggaattg ggaatggagt
    36061 tgcttgaaaa ggttggacta gcagataaag ctaatgccaa tccagatagc ctatcaggtg
    36121 gtcaaaaaca acgtgtggcc atcgctcgtg gcctagcaat gaatccagac atcatgctct
    36181 tcgatgaacc aacttctgcc cttgaccctg agatggttgg agacgtactt aacgttatga
    36241 aggaattggc tgagcaaggc atgaccatga ttatcgtaac ccatgagatg ggatttgctc
    36301 gtcaggttgc caaccgcgtt atctttactg cagatggcga gttccttgaa gacggaacac
    36361 ctgaccaaat ctttgataac ccacaacacc ctcgtctgaa agagttctta gataaggtct
    36421 taaacgtcta aactcaaact gtaaggattt ccttgcagtt tttctacctc gtattggaat
    36481 ttttgatttt tcggaaaatt atgttagaat taagtttatg aaatgaggtt tcctcatacc
    36541 tagcaagact aggaataaaa atagaaatta ggtagctaga tgtcatctaa ggttattgtt
    36601 acaattttcg gtgcgagtgg agacctggct aaacgcaagc tctacccttc cctttttaga
    36661 ctatataaat ccggcaatct ttccaagcac tttgccgtta ttggaactgc ccgtagacct
    36721 tggagtaagg aatattttga atctgtagtt gtcgagtcca tccttgattt ggcagatagt
    36781 accgagcaag cccaagaatt tgctagccac ttctactatc aaagccatga tgtcaatgat
    36841 tcggaacatt atattgcttt gcgtcaatta caagctgagc ttaatgaaaa ataccaagct
    36901 gaacacaata agctcttctt cttgtctatg gcacctcagt tctttggaac cattgccaaa
    36961 cacctcaaat ctgaaaacat tgtcgatggc aaaggttttg agcgcttgat cgttgaaaaa
    37021 ccatttggta cagattacgc aactgcaagc aagttgaatg acgaactcct agcaacattt
    37081 gacgaagaac aaattttccg tatcgaccat tatcttggca aggaaatgat ccaaagcatc
    37141 tttgcagttc gctttgcaaa cttgattttt gaaaacgttt ggaacaagga ttttatcgac
    37201 aatgttcaaa ttacctttgc ggagcgcttg ggtgtagaag aacgtggtgg ctactatgac
    37261 caatccggtg ccctccgtga catggtccaa aaccacactc tacaacttct ttcgctcctc
    37321 gccatggaca aaccagcaag cttcacaaaa gacgagattc gtgctgaaaa gattaaggtc
    37381 tttaaaaacc tctatcatcc aactgatgaa gaactcaaag aacactttat ccgtggacaa
    37441 taccgctctg gtaagattga tggcatgaaa tacatctctt atcgtagcga accaaatgtg
    37501 aatccagaat caacaactga aacctttaca tctggtgcct tctttgtaga cagcgatcga
    37561 ttccgtggtg ttcctttctt tttccgtaca ggtaaacgac tgactgaaaa aggaactcat
    37621 gtcaacatcg tctttaaaca aatggattct atctttggag aaccacttgc tccaaatatt
    37681 ttgaccatct atattcaacc aacagaaggc ttctctctta gcctaaatgg gaagcaagta
    37741 ggagaagaat ttaacttggc tcctaactca cttgattatc gtacagacgc gactgcaact
    37801 ggtgcttctc cagaaccata cgagaaattg atttatgatg tcctaaataa caactcaact
    37861 aactttagcc actgggatga ggttggtgcg tcatggaagt tgattgaccg tattgaagag
    37921 ctctgggccg aaaatggtgc cccacttcat gactataaag ctggaagcat gggacctcaa
    37981 gccagctttg acctacttga aaaattcgat gccaaatgga cttggcaacc agatatcgcc
    38041 tatcgtcaag atggtcgttt cgaataaaaa aatcctgcaa gtttgtacct tgcaggattt
    38101 ttgcttctga ttagattaag ccttccaaga gacctttcat aaagttttct gagttaaact
    38161 ctccaatatc atcgattttt tcaccaaaac caatcaattt tacaggaata ttgagttctt
    38221 cacgaatggc tagaaccaca cctcctcgag cagttccatc aatcttagtc aaaacaattc
    38281 ccgttaaagg tgtgattttc gaaaattctt tggcctgtac tagggcattt tgacctgttg
    38341 atgcatcaag tgccaagaag gtttcatgtg gtgcttctgg cacaacacgt ttgataatac
    38401 gaccaatctt ttccaactca gccataaggt tatccttatt ttgcagacga ccagcagtat
    38461 caatcatgag aatatcgata ccttcagcca cggcacgttc cataccatca aagaccacgc
    38521 tggctggatc agctttttca ggtccagtta ctactggaac atctactcgt cggccccatt
    38581 cagctagctg agctactgca cccgcacgga aggtatctgc tgcaaccagc atgaccttct
    38641 taccagttcg tttgtagcgg tgggctagtt ttccaataga agttgttttc ccaacaccat
    38701 tcacaccaac aaagagcata actgtcaagt tatcttggaa gtggatgctt tcatcgtagc
    38761 taccatcctt ttcataaagc tcaaccaatt tctcaatgat gacacgacga agtgcatcag
    38821 gtttcttggc attttcaagc ttggcttcgt aacgtagttc ctccgttaag ttagaagcga
    38881 cttggacacc aacatcactc ataatcagca gttcttccag ttcctcgaaa aattcttcgt
    38941 caacagagcg gaagttagca aagaaggcgt tcaagcgagc accgaagccc gtacgagtct
    39001 tcttgagact gcggtcatat ttttcctgaa cagtttcttc tgtttgaggc ctttctggtt
    39061 caagaacttc agaattattt tcttctacag ttccttcgtg ctcaagcttc tcttcctctg
    39121 gtaattcttc tgagtttggt aattcttcta tttcttcttg agaaacccct acagctggct
    39181 ctgaatcctg actttcttca actgtgtctt ggatttcctc ctcttggaac acagcttgtt
    39241 caacaatttc aacctctgct tcttcctgag gaacttcctc aacttctgtg aaggtaggat
    39301 caacatcttc agacaaatca agattttcca gagcttcttt tacaacttct tcgattttag
    39361 gttcttcttt ttttccgaat agacggtcaa acaatcccat atcttagttc tcctttagca
    39421 catattcttc gatagcccag gcgacagctt cctcatcgtt ggtcatcggc attactacat
    39481 ttgcggctgc ctttacttca ggaacagcgt tttgcatagc aacaccaaga cctgcccatt
    39541 caatcataga aaggtcattg gcctcgtcac cacaggccat aacttggctt tggttgattc
    39601 cgagatggct gattagtttt gccaaacctg ttgctttatg aacattcttt ggtgaccact
    39661 ctagcaacat ttcacgtgat ttaaagattt catattggtc aaacaattct ggagaaatct
    39721 tctgaatggc tgcatccaag ggttcttgag caaaggcagt cacgcatttg ttgtaggtca
    39781 tttgactaga caggtcttca aagtcaactg gaacaaaagt caaagctgga ttgaatttgg
    39841 cataaagact ttcttggtcc gattggattt gataaactgt tccttctgag atggcatcaa
    39901 gtggcagtga taatttctct gttttctcat acaagcgtgc cacatcatca tatgaaaaga
    39961 ctgtcttatc aaggatttca cctgtatttt tctgaaccaa accaccatta aaagtaatgg
    40021 tatactcatc ttcctgaccg tcagtcccta gctcatggag aaagaaatcc atagctttta
    40081 agggacgacc agttgtcaat acgaccttga taccacgatc acgcgcagct ttcaaggttt
    40141 ccttggtacg atccgtcagc cttttatcag tagtcagcaa ggtcccgtcc aagtccaatg
    40201 caatcaattt tatatctgcc attataagcc ctccatataa gctataaccg accgttcctt
    40261 atggtgacca atcacagtct ttgctaattc taaaatttca ggtcgtgcat tttcaggagc
    40321 tacaggatgt cccacaacct gcatcatatg taagtcatta agattgtctc caaaagccat
    40381 gacctgatcc attgtgatac caagtttttt agctaattca acaatagcta cacccttatc
    40441 gacatagtcc agaacaatat caatagattc aaagccagtt gtcatagcct tgacaccagg
    40501 gacattatca ttgacccaag cttcaccagc ttctagcgtt tcttctgtga agttggttgt
    40561 aaatttgaaa atgtcatctg tgatatcttc caaactcgct actttttgga tattttcatt
    40621 ataatgctga ctcactttca aataggtctc atcaaccgta tctagaacat atgaaccctt
    40681 cttacccgtc aagagcagtt tattgatatc tacataaggt gacgttttca gcttttcaaa
    40741 agttgccaga taaaagtcac gagacatagt cgcttcatac aagtcctgac cttgatactc
    40801 taccaaactg ccattttccg cgatgaaaat aatgtcatca cgaacaccag caaataattt
    40861 ttccagagac agaaatcccc gacccgaagc caccgcaaag taaatccctt tttccttata
    40921 ggaagccaag agagacttga ggcgatccat atcaaagcgc ccattcccat ctaggaaggt
    40981 tccgtccata tccgttgcta ctagtttaat tgtcatcctt caatactttc taaatctttt
    41041 aacttaaccg aaacaatttt tgagaccccc gattcttgca tggtcactcc atagatagaa
    41101 tcagccgctg ccatggttcc cttacgatgg gttacgacga tgaactggct gtccttgtca
    41161 aagcggttga ggtaatcccc aaaacgttta acattggctt cgtccagcgc agcctctacc
    41221 tcatccaaga tgacaaatgg gatagtcttg acacgaataa tggagaagag caaggcaaga
    41281 gccgatagag ctttttcacc accactcatg aggttaagcg actggatttt cttacctgga
    41341 ggttgaacag aaatctccac accagctgtt aaaaggtcgc cctcagtcaa tatcaagtct
    41401 gcctgacctc cgccaaacat ctgcttgaag gtcactttaa aggactcacg aatggcctca
    41461 aaggttgatt taaagcgttc cttaacctca tcattcatct ctgtaatggt ttcaaggagc
    41521 agatttttcg ctgacaaaat atcatctcgc tgactattta gaaagtccag acggttgtga
    41581 acttcttcgt actggtcaat agcttctata ttgacaggac ccagtgagcg aatagccttc
    41641 tctaaatcct taacttcttg ctctgccaga ttgaggtttt ccaattcatg cgctttttct
    41701 agagcttcag tatagctaat ctggtactgg tctgttaatt gactttgtag atggcgcaag
    41761 cgttcgctga ccttttcttt cttggcttca gcacgtgttt gcttgcgaat ccactcctca
    41821 ttctgctggc gagcctgatc caaatgactg gcaatatcat ccagttgacc ttctatatca
    41881 tccaactcaa actgctggcg aatcaaacct tgttggagat ttgttttctg agttttggat
    41941 tcttccgcct gttgactgag caattctgta tcaaccttct caagattatc aaccttttct
    42001 tgaagaaggc gctggatttc ttcttgttca aaatcaagat tgtccaattc cttgcctaag
    42061 cgttcaatat cagcaacttc ataacgtttt tgcccttgca gttctgtctt aagcaaacga
    42121 gcttgcgcta gctcttcctg caagttttga tagcgttctt ggatggcatt tttgttagac
    42181 ttaatctctt caatctcagc ttccagattt tgcttgtcac tggcgattgc agcaagacgc
    42241 tcttggcatt tttccttatc cgcttgccaa tctccctcgg aaagacgatc tatttcctct
    42301 tcttggagtt tccaaagagt ttccagttct tcaacttgct gactggtctg ctgataggcg
    42361 agggacaagc cttgctcctg aatacgagcc tgctctccct gagatttgat ggcttctaat
    42421 ctttctgtca atctagctag ctcatcttgc aagttcttca aactcacttc atctgaacgc
    42481 aaacttgctt cttcttcagc aatttctttt tgtaattgct ccagttctgg cttgatgaaa
    42541 atactgttgt tctgacgatt ggctccacct gcgtaagaac cacccgtgcg taattctgtc
    42601 ccatccaatg tcaccatacg aacctgataa cgaacttggc gagctgctgc acgcgcatgt
    42661 tctacggtat caaagatagc cgtcgtagct agcaagttct tgaaaatggc ttccagtcta
    42721 gtatcaaaag tcaccaactc atctgccatc ccaaggaaac ctgggcttac agcgatagca
    42781 tcttggttct gactagaaat cgtacgcgcc ttgatagtgg ttaaaggaag aaaggttgca
    42841 cgaccggctc tgttccgttt aaggaagtca atagccttgg ttgccgactc ttcatcttct
    42901 acgatgatat gctggctact tgcccctaag gcaatctcta gggcagtttg ataataaaca
    42961 tcaaaggtca gatgctcact gactgcacca ataatcccac ctaggcgatc tttttcttgg
    43021 agaacactct taacacctgc ataaaagtta ctatgatttc tcaggatatt ttccaaactt
    43081 tgagctctgg cctgcttgtt tttgagatta tccagacggt caaagagttg gctttgttga
    43141 gcttgatagg aagttttctg ctcctcttgc tccttggcaa tagcttggta gtcagccaat
    43201 aatttctgaa cctgctcctt ggcagtttca agctcttcct tttgctgact agccttctct
    43261 ttagctatag ccaattgctc tttcagcttt tctagttgat ctgcttgttt ttgagaaagc
    43321 tgacgactat tttccaactc attctcaata cgggtcaact ggtttgagac atccgcttct
    43381 tcttgtaaaa gagctacaaa gcgttcacgt aagagctcaa tcatctgatc aggatcgtct
    43441 gagaaagcca gcaattcagc ttctaaacga ttgagttttt gattattttg gactagattt
    43501 ccctctaaca gagctaaaga gctttcttta tcatactttt ctttgctgag tgaatttctc
    43561 ttatcctcca aagcagccaa acgagcttgt gcctcctgtt gattcaaggc cacttgctcg
    43621 gactccagtt tcgatagggc taattttctt tctaaatcac taatcagact agttaagtcc
    43681 atcaaactgc cttggtcttt ggccatttca tcctgtaaat cttggcgttg ctttttaaga
    43741 gtttgatttt cttcttctaa tttttcacgc ttttggtaat aactcatcaa gagttcttga
    43801 acctgagcca gctcttcttc tgtcgactct agttctgcct tattttcctt gatttgagca
    43861 accagaacgt ctaaataaat agccttacgt tgtccttcca agtctaaaaa cttacgggca
    43921 ttctcagctt gcttctcaag aggcttgatt tgattatcca actcgtagat aatgtcctct
    43981 aagcggtcca gattatcctg agtttgctgc agtttactct cggtttcttt tctgcgagtc
    44041 ttgtatttta aaactccagc agcttcttca aaaatagctc gtcgttcctc agacttggaa
    44101 ttaaaaatct cctcaacctt cccttgggaa ataatagaga aggaatctcg tcccaatcca
    44161 gtatccaaga agaggtcatg aatatcacgc agacggactt tcttgccgtc aatcttgtat
    44221 tcgctatctc cactacgata gatatggcgt tccaccctga tttcttgacc tgcatccttg
    44281 ataaatccgt catgattatc cagagtcaca actacagaag cataattgag cggtttgcga
    44341 ctttcggttc cagcaaagat gacatccggc atcttgcccc cacggagact cttgacacta
    44401 gactccccca aagcccaacg cagactttct gtaatattgg actttccaga tccattgggt
    44461 ccaacaactg ccgtcacacc ttggtcaaaa acgaccttgg tcttatcagc aaaagacttg
    44521 aacccctgaa tttcgatttc ctttaaatac atgaatccag ccccttctca acggcatttt
    44581 tggcagcttc ctgctctgct aatttcttag aacgaccttg gccttgaccg atgctcttac
    44641 cttcaacaag aacttctaca tcaaagacct tatcgtgagc aggccctgtt tcagaaatca
    44701 cctgataacg aatagccaca tcaccattga cctgaagcaa ctcttggaga taggttttat
    44761 agtctgtaat catctcaaac tcgcctgctt caaccttagg aatcatgact tgatagataa
    44821 attccttgac cttggccaca tccttatcca aaagaagggc accaagaaag gcttcaaagg
    44881 catcaccaag aatggtgtca cgattgcgac cacctgattt ttcttcccct ttacccaact
    44941 tgataaactg gtcaaactgg caatcacgcg caaaaccagc taaactctcc tcacggacaa
    45001 tcatagcacg gagttttgat aggtcacctt caggcttttt aggatatttt ttatacagat
    45061 attctgaaat caataactgt agaacagcgt ctcctaaaaa ttccaagcgt tcattgtgtg
    45121 aaatttttaa gaggcggtgc tcattggcat aactcgtatg agtaaaggca gtttccagta
    45181 aatttttgtc tgtaaattcg attgcaaaat ggttctttag tacagtttgt aattctttca
    45241 taccaacctc tttctaactg ataacagtcc tttttattat atcaaaaaaa gccccctgag
    45301 tcactctaaa acgggactgg aaagcatttg ggaattcttt agacagagat tctcagtttt
    45361 agcggcaaat ttagagcagt gtaaagaaaa aagccctatt aaaggctttt taggatgttt
    45421 acatccaccc tgagggaatc gaacccccat ctcaagaacc ggaatcttac gtgatatcca
    45481 ttacactaag ggtggaaact tgttttatta taacagaaat ttgctctaat aacaagtttt
    45541 ttagtcaaag accccgtctt agtgggaagc atccccattc cagattgagt ttttcacgat
    45601 cacataatca acgtgtttaa ggtcagcaac ctgacgtcca cctgcataag aaatagcact
    45661 ttgaaggtct tgttccatct cagttaaagt gtcttgcaga tgacctttag caggaagcaa
    45721 gatacgtttg ccttccacat ttttgtaagc acctttctga tattgtgagg ctgaaccata
    45781 atattctttg aactgttcac catcgacttc aatcgttttc cctggacttt caatgtgtcc
    45841 tgcaaagagg gaaccaatca tgatcatgct agcaccgaag cggatagact tggcaatatc
    45901 accgtgagta cgaattcctc catcagcgat aatcggttta cgcgcagcct tggcacacca
    45961 gcgtagagca gccaactgcc aaccacctgt accaaaacca gtcttaacct tggtgataca
    46021 aaccttacca ggaccgattc cgaccttagt agcatccgca ccagcatttt ccaattcacg
    46081 cacagcttct ggtgttccca catttccagc aatgacaaag gtatctggca attctttctt
    46141 gatgtgttga atcatagaaa tcacgctatc cgcatgacca tgagcaatat caatagtgat
    46201 atactcagga gcatcagtct tgagctgtct aacgaaatca tactcatagt ccttaacacc
    46261 gacagagata gaagcaatga gcccttgatc gtgcattcgt ttaataaaag gaatgcgtcc
    46321 tgcctcatca aaacggtgca taatgtagaa gtaaccacct ttagccagtt gctctgctac
    46381 attttcatcc aaaatcgtct gcatattcgc tggcacaaca ggtagtttaa aggtgtgatt
    46441 tcctaaagtg acacttgtat ccgcttctgc acggctttta atgacacatt tatttggaat
    46501 caattgaata tcttcgtaat caaaaattgg aaattcattt aacatatcga tgtctcgttt
    46561 cttttgtaat gacctaccta tgctctcgca tcactacgtc ttttccgacg tttccttaat
    46621 ttattataaa ccaaagtaca gtttttgtca aattatttta tgaattgaaa tatattgttc
    46681 gggttttact tgtgttaaaa ataaaagaaa gagaggaaat tattttctcc ccacttcttc
    46741 atcaataatc gctttaaaat tttcttctga attttccatc attttacaaa atgcactata
    46801 agatgaagac ttctgaggaa tttggataga ccatttcttc aacaaagctc cataacctgc
    46861 taggtttccc atctcattat caataacctt gtccttgctc ggaaaaacaa gtccagcctt
    46921 ctcagctttt aaaatataag aataggaaat cagctggaat aagtcctcac ggttgattcc
    46981 tttttcagtc aattccagtt ttttatattt tgcatctaga acaatctttc gttctctgtc
    47041 ataaaaatct ggatatactt ttcgtttccc aacagaaaat actgaaattc catccgtctt
    47101 atccttattt ctgggatgta caaaaccttt tggcaacaag gtgtaaacat actcttccca
    47161 aagccaggca acatcaaaga gaataccata gattttttga tcttgatacc ctaaaccgtg
    47221 cttttcttgg tttaggatca tcagacaaag ttcttgtaag tttctgtact cgtgaaagta
    47281 tgcatgacgt ataggtttag attgatttcc ccgaataatc ttagcacgat cagctagttt
    47341 ataagagggc gttacacgca cgatttcaga tacgttttca cgactagttg agagattatc
    47401 tagtacccct tgaccaatgc ttttctgatt cttaatgtat tcaatagtgt gacggaccaa
    47461 ctgcatgagg ggattatcat aggtgaactc tctcgttgtg taggcaatat ttcccgtgaa
    47521 aggaagattt ttcttgagat ggtttcttac atcaatcact cccttaacat gactgtcgtt
    47581 atgagaaaat ctctggtatt ccttataaag accttttcga atagcagctt gtagatactt
    47641 ggggaagaga tacatcaaaa gttgataaag cctctcttca cgagacaaag caacatctaa
    47701 actagtgaga ttgatatgaa gaaccttgtt taagagataa tgcaaaaaat ggtcattact
    47761 ctcatcagaa aatcgtgagg aaatcgttaa tctttcctga ccatatccaa gaaaaccaat
    47821 cacgttccct gtcttgattt tctgattgac tgtttcaaaa atcttttggt ccttatccaa
    47881 atcaggagta tgagtcaaat catttggaaa aatgaaaata cgttcatctt gggatagact
    47941 ttccaaggtt ctgtctaaaa gaagattgct tagtttagga tattcttcaa caaatttttc
    48001 tttaataatc ttgtattgat tatcagtaat ccgcatcatt atcaccagta tcttgttgac
    48061 cagtttgatc attatttgtc agttcaaatg cttttttcaa agtttccaaa gtttcaacct
    48121 catcataaga acctcgcaag tagtcttcta ggagaggctt aatataatca gaccagagta
    48181 attcatagtc aaaatctaca tccttcaact taagaaaata acttggtcca atatgataat
    48241 gactgtttaa ttcctgaaca ttttcgatag cagcgttcaa gtttcttaga cgaatttttg
    48301 cttcttctgc atggatattc agctctttat ccaacatgcc aacttgaccc tcggcagtaa
    48361 cttcaacaaa acgaaaacga cgacgcatag caaaatcaaa ggtatccact gaacgatcaa
    48421 tatcattcat agttccgatg atgtaaacat tttcggggat atagaacttt tcatcagttt
    48481 cgtgtagatt tgcatattgg gtagaaacac ttcctttttc accacgatag ccagggtcga
    48541 tagagaaaaa gagttcgcca aaaatcttag aaatctcccc acgattgatt tcatcgatga
    48601 tgaagacgaa tttcttatca gtatcaattt ctgttgggga aacatagtct ttcaaaccaa
    48661 atctctttct caatgtttct aggacagttt ttccaccact tttgtagtat tcttgcttat
    48721 tataattttt atctttatac aactcgtaaa catatttgct aggtagtgac catcctgaaa
    48781 caccactatc ataatttact gacaaatttt gtctactatt aatagataag taagatgttt
    48841 ttgttatata ttctttttct tcagcaacat ttatatattc taagtaagaa tcccaagcct
    48901 catcaaaatt atcttgtcct ccaatcaatt gggtttcttt tgctttctga caaaaatctt
    48961 taaaaatacc gtcctgtagc ctaaactcaa tagctccatc cccatttgat actggtctta
    49021 aaccttctac aaaatccgta taatcatagg atgggtgaaa ttgtacaaat ccgatttggt
    49081 cttcgttgcc atccgttaat tctttggcaa tttctttagc aagataagtc tttcctgtgc
    49141 caggagcacc acggaggatg aggtttggag actttaatag agattgagtt aattgatgac
    49201 gaaaattgtt tgatgaatcc attgtactat tgtcttcctt attttccata aaaagcttag
    49261 aatactcaaa aagcatagtt cctaatttcg atggctccaa ctcaggataa gttttggaca
    49321 ctagttcaat tattttatga ttttgcagat aaggtccacc ttgatttgtc tgagaaacaa
    49381 agtggttcca aaattctttc tgactattaa tatttacacc aaagaaagga tctccctcag
    49441 tatagataca aagtaatttt gtcaccatag cagaacgacc aataaattca tttgttgatc
    49501 ttttcatatc aaaaatagga ttttcaaagt taaaacgaat accttctttg ataatttcat
    49561 acaaatctga ttttaatttt gaaaatcgct gatccaactc tgaaatctca ttattagctt
    49621 gatctttata tttgtttgtt tttttattcc aataaatacc aaattttgaa gctgagccac
    49681 cagaaattcc aagaaataga tttttgtatt ttcccttctc aagagcgtag cataaagact
    49741 tattttgctg tccctttcct attacatatt catcgattga cattgttaaa atcctatcca
    49801 aaggccattc ctggagaaac tcttctttta atttatcatt aaacttttca ccaacagttg
    49861 ccttatttaa taactcttct ttatttagac tataccattt ataaaaatct tctaaactaa
    49921 cttttctttt attcatcaat ctaactcctt ttactaatga tgtttaaata ttggcattac
    49981 ttattaacat atattttcta catagaatac catatcctat agaaaaagaa aagaaattct
    50041 aaacaaaaag tccagtgtta ggagctttaa tatctcataa cactagactt ctttttttat
    50101 cttaataata ctcaacctga cggtagtccc atgagttaaa agtatctgac aggtgcatca
    50161 tgattttatc aatgtcaagc cctttacgga tcacaactgg tgctggtgaa gttgaacgtg
    50221 ctcctccttg ttctgggatg agtttgccgt ccttatcgac catagacacc attacacctt
    50281 gctctcatag cgagtttcaa tcgttttgtc attgaagcat atgaggcgta gaccatttta
    50341 ccttcttccc aaagcttggc agccaattgt tcacctgctt ttccaaactt gataacaaaa
    50401 cagctagtac cacgtacatc agtcataagt tcttcacggc cattcccgta tgctttgagt
    50461 ttttcaaagg cttctggttt ccaaggaagg atacaaccaa gaagaatatc ttcatccaat
    50521 gtttttagta gaatgcttca atttctgggt tgagttgcgc taaagcgcga agttcatcca
    50581 tgctaccgca gagaacaaca cctggtttgt tacgcttacg ttctttggct gcgaacttac
    50641 gctcaagtcc tgccttgttg ctggtcatga taatgtaacc aactttagta gggcaaacga
    50701 tacatccgcc ctcacctttt aaaatgtcgt agccttcttg tgaatgtgtt ttgtcataat
    50761 tttataatgt gttgaatcca gaatagtcca ctgtagtttc taaaaaattg ctggaaatgg
    50821 atgttaagct ctaattcatt tgtttacatc ttatttcagt ttactatatt tccttttcta
    50881 ttttttctaa tcctgccagt aggctggtcg ttgtttttca taagcagcaa tcaactcttc
    50941 atactgcaag gtaataccga tatcatccaa actatttagg agtttatgtt tccactcgct
    51001 atctatctcg aaggtgaatt cttcaactgg tgagatgatt ttttgttgtt ccaagtccac
    51061 agttacctgg tcggttggtt ttagctgggc tagtttctct ctaacctctc taggctgaac
    51121 gattggcaac aggccattat tgagttcatt attgtaatga atgtcaccga aagatcctgc
    51181 aatcacgacc ttaaaaccat agtccgctag aacccaagca gcgtgttccc tcgaagaccc
    51241 tgccatccat tacctccaaa taaaatataa ggctactagt gtggtcacca tcccaagaaa
    51301 ccaaactgtt ttaaagtacc attttcgagt cttgtggtga aaagtgattc cagctaacca
    51361 ggccccaaaa ccaccacaag taagggctaa aatgagtaag attttctctt ggatgcgcca
    51421 agttcttctt cttgccttga atttgtcaat gccataaatc aagaaaatga tgacattcca
    51481 aatcaaaagg actagagtaa ttttttcgtc taacttcata accttgcaat aatagcttcc
    51541 gtcatttcct ttgttgaagc ctgacctcct atatctctcg ttaaaattcc tgccgccaga
    51601 cttgtctcaa cagcatgttt gatacgctct gcatcctcat aacgtccgaa actatctctc
    51661 aacatcatgg caactgataa aatcatggaa gtaggattgg caattccttg acctgcaata
    51721 tcaggtgctg aaccgtgaat aggttcatag agacttggtc cattttcaga atgactggct
    51781 gatggcataa cctcaagtgt accagataag acgcttgatt catcagataa aatatctcca
    51841 aaaagattct ccgttacaat aacatcaaac ttagcaggat tggtaatcat aagcatagca
    51901 gctgagtcta ccagctggtg ttccaaggtt acatctggga aatcctgtgc gacttcctca
    51961 gctactttcc gccagagttt tgaggtcgct agaacatttt gcttatcgat actagtaacg
    52021 atttttctgc gatttcttgc aatttcaaag gctttgcgaa taatccgctc cacttcctca
    52081 tagctatagt cgttgatatc acgcgctttg cgctcttcaa gaatatgatc tccaaagtaa
    52141 atctcgcctg tcaattcacg caccacgaca aagtctacac cagaaattcg ttccggtttg
    52201 agtggtgaca aatacttgag actgtcaaag atttttacag gacgaatatt agcgtaaaga
    52261 ttgagttcct tacggagagc catcaggcct tgttcagggc gaaccgctgc tccatcatac
    52321 tgaggactac cgatagctgc tagtaggata gcatctgctt ccctacttgc cttaagggtt
    52381 tcatcaggta agggaggtcc tgctgcatca atatctgcac ctccgaacgg tcgtctgtca
    52441 atctcatagt caaaacctgt tttttcagct agagcctcca gaacttctaa accagcctcc
    52501 atgatttctg ggccaattcc gtctcctact agagctacta ttttctttgc catagccttc
    52561 tcctttacac actaggcata tcgtggtaag aaacactgcg tcccatctca cctgcattct
    52621 ctttttgaac aaaggtatta gcgtttatat aggcaatagc agaagccttc aacacatcaa
    52681 aatcaagccc tgctgcatta aagatggttt ctgtatctct gttttcaaca gtgaccaaaa
    52741 cccgatcctg ggcatcgatt ccatctgtta ccgcattgat agtgtaggac accaaacgaa
    52801 cagattggtt aaagaactta tcgatagcgt taaagattgc ttcaacggaa ccttgccctg
    52861 tcgcattaaa ttcgactttc tcaccatcca tattggctag gctaacgagc gcttcaatgt
    52921 cattatctgc atgagtttga agttgtaaat catcaaagtg gaagccttct ggattttcaa
    52981 ccatggttcc agctaccaaa gctcgaggat ctgcatctgt gatttcttac ttcttatcgg
    53041 ccagtgcctt gaacttagca aagagtggtt tgatatcctc ttctgtaaaa tctagggcca
    53101 attctctcag tttctcaaca aaagcatggc gaccagataa ttttccaagc ggaatcttaa
    53161 caccaaccaa ttcaggtgtg atgatctcat aagtgagagg atttttaagg actccatctt
    53221 ggtgaatacc agattcgtgg gagaaggtat tgccaccaac gacggctttg tttttaggaa
    53281 ctggaatacc agagaagcga gaaaccattt ctgacgtatt gatggtctca tttaggacaa
    53341 tactggtttc tacttggtag taatcttggc gaatattgag agccactgca atttcttcca
    53401 aagcagcatt ttcagctcgc tccctaatac cattgatagt cccttcaaca cgtcctgcac
    53461 cattcttgac agcagcaagg ctatttgcca ctgccattcc gaggtcatca tgacagtgag
    53521 gcgaacagat gatctgacga tccgtcttga cattctcaat caggtatttg aagatggcac
    53581 catattcctc tagtgtggta aatcctatat tttctaaaaa tttcttcagt aaagaatatt
    53641 tagctaattg aaagttcatg aaaattatta aaatatttca ttttttagag gttaagttcc
    53701 aacttttttc tatcaattcc agtacttctt catctgataa agtatcatca agggacacac
    53761 taatccagta gcgcttgctc atatggaagg ctggataaat ccccttttgt gaaagcaaat
    53821 tagctacttg gtcatgcttg aggttgactg cttccacttg tccttctctg cccttttcca
    53881 gcttattcca agagattttc atcaagacgg cataccactt ttgattgcct tcatggcgca
    53941 atacagctgt atcaggcgat ttttcccaca gatactccaa ctggtttcca tacttttcct
    54001 gaacttgagt catgatacgc ttagtctgat gacagataaa atcttgcaca tcaaaacaag
    54061 ccttccgaat ctggtaaaga atctccagac aagcctcacg gacatttccg acaaaacttc
    54121 ccctcatgct ttccatatga acgtgaggat agaggtcacc agtctcttgg tcaaagactt
    54181 gaaagttcac attatcagca gtgatggaca cagtcatgac aaagtcacct tgcaaaatct
    54241 ggcaactata tgtccagact tcgctatttt ctataaaacc ataatcatga gccttttctt
    54301 gattaaattg ataggattta aaaatttcaa acataagtga aaactgctac ccaaggctag
    54361 cagttccttt ctatttttta aaaaacaacc ttagtaccat gcaattgtgt tacccccacc
    54421 tggtcaataa aggtttgacg gttgtcaagg tcaatccccc cacctggtag aatttcaatt
    54481 ttacctttag cgtactccaa aattctgtga tagtgaacaa aacgtttttc taaggagtcg
    54541 ccagacacac cagcacgagt taggatacga gtgacaccgg cttgactgag ccagtcaata
    54601 gcttccggtt gatcttcatc acttagttca tcaaaggcca tgtgaaagac aatttccatt
    54661 ccttttgatg cagcaattaa cttttccaga ttaggcttat ccaacttttt atcagcagtt
    54721 aaagctccaa atacaacccc ttgacttcca gcctgagcag tcaaacaaat gtcttctagc
    54781 atgatagcaa tttctaggtc attatagaca aagtcaccac cacgtggccg aatcatggtc
    54841 atgatggttg tatcgtagtt agctgccagt tcaaccgctg ccttagtcac tccatagcta
    54901 ggtgttgtcc caccaactgc tagattatca cagagttcaa tccgacgagc tccagcctgc
    54961 atcgcttttt caagtaaagt cacattttca gcacaaaatt cgtaaatcat ttgattctcc
    55021 ttgaatatta cttcaaattt attatatcat attgttatga atatgctttc aattttatca
    55081 aggtaaataa ctactgtttt aactattctt tgtctggaat gaccttgaag agataataag
    55141 tgataaagat ggtcaaagca cccagaccga ttttgactgg taagagaggg gcaaaataaa
    55201 tagaaatccc catcaaaatg taaatggata cgatgatttt tttctttcgt tcacgcgtaa
    55261 tagacttagt ctcgcgaaaa tcagctacat atgtttgata gagcttggta tgataaagcc
    55321 agtcttcgaa tcgcttggaa cttctggaga aacaagcaat agacaacaaa aggaaaggtg
    55381 ttgtaggcaa caagggtaaa acaaccccaa caatagccaa ggccagtgat aaaaaaccaa
    55441 ttatcagata gataagacgc ataacttctc ctagttaaaa taatcttctt ctagtttcgc
    55501 ataaaatgat gtattttgtc aagttctaac caaaaagagc ctgaaattta ctttcgggcc
    55561 cctcctctta tttaatcttt tcttcctcgt agtcgccttt gctacaaaca acctgcttgc
    55621 caccaccacg gacttttttc tccatgagga agttgccaca ttttggacag tcacgaccaa
    55681 caggcttgtc ccaagaggta aattcacatt ctggatagcg attgcaacca tagaagaggc
    55741 gattacgctt ggtttttcgc tcaataattt gtccctgatg acagcttgga cactcaacac
    55801 caatctcttt cacgattgct tgggtatgac ggcaatctgg gaaattgcta caagcgtaga
    55861 atttaccaaa acgaccaagt ttaatgacca ttggactgcc acacacttca cagtcaaatc
    55921 cagctggttc atccttaatc tggatttttt ccatttcttc ttcagccttg gcaacttctt
    55981 tagagaatgg tttgtaaaag gcatcaatga cccgtcgcca ctgctctttt ccaacttcga
    56041 catcatccag tttaccttcc atttcagctg tgaaggtcac gtttacgata tctgggaaat
    56101 attcaacgat gagcttattg acaatttctc ccaactctgt cggttcaaaa cgtttggctg
    56161 ccaggcgaac ataataacgt ttctgaatgg tttcaatggt tggcgcatag gttgatggac
    56221 gtccaacccc attttcctct aaggttttaa tcagtgttgc ttcagaataa cgggcaggcg
    56281 gttgggtgaa atgttgctct ggtttgctat tgacctgttt gaccacatct ccaacaacca
    56341 tgtccggtaa catcttattc ttgtcagaat cattataaat ggcaagataa ccatcaaact
    56401 taacctgact accattggca gcaaattgaa ctccattttg agacaattta acagccatgg
    56461 tatcaaaaac ggccgctgtc atctggctag ccacaaaacg attccagata agggtatata
    56521 gcttgagctg atccttgtcc agatacttag cgatgctttc tggtgtatta aagacacttg
    56581 acggacgaat agcctcatgg gcatcctgag cacctgatgc gtttttgacc ttgctaccgt
    56641 gcttagaata cttgctacca aaacgatccg taatgaagct tgccgcctca ttttgcgcta
    56701 caggactgat acgagtcgaa tcggtacgca tataggtaat caaaccttga acaccagaac
    56761 caatattaat tccttcatag agctgttggg caaccatcat ggtttttcga gtacggaaat
    56821 tgattttatt ggcagcatcc atctgcatag atgaagtggt atagggtaaa ggagcattgc
    56881 gcttgcgctc ttttttatcc acctgatcta ctgaaaagtc tttactcgtc agacgagaca
    56941 agacttcctt gacttcgtta ttgctggtca gtttcatctt tttaccatct actccataga
    57001 aggaagcatg aaattgtttg gttccctttt taaagacagc atcaactgtc cagtattctt
    57061 ctggctggaa ggcattgatt tcattttcac ggtcaatgat gagtttaagg gcaatggact
    57121 gaacgcgacc tgctgacaag cccttcttga ccttcttcca caaaataggc gaaatcgaat
    57181 accctaccaa gcgatccaag atccgacgag cttgttgggc atcgaccaag tccatatcga
    57241 tcttacgagg ttctttaaaa gcatttttga ctgcatcctt ggtgatttca ttgaagacca
    57301 cacggttggc atcattttca tccaagttga gaatatgggc caaatgccaa gaaatcgctt
    57361 ctccttcacg gtccgggtca ctcgcgagaa aaactttatt agctttttta gcttcttttt
    57421 tcaagtcatt gataagaggg ccttttcctc ggatattaat atattgcggt tcataattat
    57481 tttcaatatc gacggacata ctggatttct tcaaatcacg gatatgcccg acactggcta
    57541 aaaccttgta gtttctgcct agatattttt caatcgtctt ggccttagca ggcgactcca
    57601 cgatgactag atttttttta actgttgatt ttttcttttt tgttgccgta gccacagtat
    57661 cacacctttt caaagtaata aactttataa agtgtaaacc attttttgaa ccctgtcaag
    57721 acttagctac tataatagaa gaaaagtttt atacttaaaa cctcagtctt cctttgatat
    57781 acatagaaga ctcacgagag aaaccagcta ttctcaagaa attattgctc ctcagactct
    57841 gtatcaaatt tattggcttt ctccttttct tttgcttcct tatccttgat atactggaag
    57901 aaatactcca ccagctcttc cttactaagt tgttctgaat cagacaattg tgaaacttga
    57961 acagaaacat cacttaaacc atagtcacct ctcttacttg aaatcatatc gagttcttct
    58021 tctgtcaaat aatttcctga aatggttagc tttagggtat gagtcttttt agaataggtt
    58081 tttttcaaaa tattataccc ctgaaactgc tctgatataa atttcttaag ggactctttt
    58141 ttcaacgttt ctctcactaa agtcgttgca gagatgaaac tcggaatcat cagcaaaaca
    58201 gcaactagaa gcaaaatcct acgcatttta gagtcttctt cattatcttt aaaataatgt
    58261 ttcttaacca tcatcaacct aacacctata taagtcgcaa tgacaataaa gctacaattg
    58321 atgaggaata ggtaagagga gcctatgata aattttagat tagcagaagc aatagcataa
    58381 cctactgtac aaagaggagg catcaaggcg gttgcaatag caacaccagg aacaatatta
    58441 ttggtctctt ttttcctagc accaataata cctgctatcc ctcctacaaa agcaatgaga
    58501 acatcccaaa tagtcggaga ggttctagca acaatctccg aactagcata cgaaatggga
    58561 gaaagataaa aataaagtgt tgaagctatt agactggcaa gaatttgaat agctaatatt
    58621 ttaaaagatt ttcttaacaa tttaaaatca aatatagcta gagagagccc cactcccaga
    58681 ataggtgtca tcaaaggaga gattaacatg gctccaataa tcacgggagt cgaatccata
    58741 tttaatccga tagaggcgat aagcatggca catagtaaaa tcgctaaatc acttctggaa
    58801 agttctaatt cttcatatat tttatttcta aattctatca acgaatacag tttattattt
    58861 gtcacaaata tcttcctttc ccagctaact agtcgctaaa atcctaacaa actcaatact
    58921 ttagtagtct gatatgccaa tctcacgagc tctttttatc attttccata atatcctgac
    58981 tcaaatgtct actgatatct ctcatcagtt tcatttctag taaatgaaaa tcaaactcgg
    59041 aagctaaccg tagcttgctc aaaacacagc aaggcaaagc tgagctgacg tagtttgaag
    59101 agattttcga aaagtatcat catctatttc agaccatttt ttataataac cattttattt
    59161 ttatttgtca aggtctttga attctttctt aaacaagcct tgtaatctct acttttgaag
    59221 aatttatttt tccttactga caagatttga gacggtagga atcattgaac ataacctagc
    59281 caacatcaat cacaatcatt tctcctttct caattacact aaattatagt gtattgaatc
    59341 tataacagtg caccttggct gctaaaatat ttctataaat caatttgact ttcctgatag
    59401 agttgttcac atcttatttc aattcactat actttccctt atactcaatg aaaatcaaag
    59461 cgcaaactag gaagctagcc acaggttgct caaagcactg ctttgaggtt gtagataaga
    59521 ctgacgaagt cagctcaaaa tactgttttg aggttgcaga tggaagctga cgtagtttga
    59581 agagattttc gaagagtatt atatgagaaa aatcctctac tctactaatc cacttagaaa
    59641 aaacgcatat tatcaagtgt taaaaacttg ctaatatgcg ttttattatg gaaatgttgg
    59701 gttcattttc tcttaaaatt gattttttct tagaattcta gcttaggtca tttttaaaat
    59761 tcaaattccg caagaacatc ttgcccactg gtgactaatt ttgctccttc ttgaatcaaa
    59821 tgatggcaac cgtctgatag tccatctaaa atgctaccag gaatagcaaa gacatcgcgt
    59881 ccttcttcca ttgctcgctc acacgtaatg agactacctg aacgcatctt agcctctgct
    59941 acaatcacac cacgacaaag tccagcaatg atgcgattac gggcaggaaa atgaaatttc
    60001 agaggttgtt caccaggtcc atattcactt agaaccagat ggtcattgcc gatgtagtct
    60061 tgcaagcgtt tattggcttt aggataaaac acatccagtc ctgttccaat cactgcaatg
    60121 gtttttccgc cattctgaag agctgccata tgagctgctg tgtcaatgcc cttggccaga
    60181 ccactgacaa taaccagttc attttccaag ccttgaatga ctttttcaac tgacttagct
    60241 ccctgtttgc tacaagcacg actgcccacg accgctacct tcgggaattt caggaggtca
    60301 agatttccct tgtaaaataa aagtacaggc gcatcatata tttcactcaa atcccaagga
    60361 taacagtcat ctaaaataga gaaagatgga aatttttgaa actctttcga caaatgcgca
    60421 tcgtctatct gaaaataacg ttccataaaa acggctggat tacggcaacc tgagatatct
    60481 gcaatatcac ccaacaaaag ctcctgatca acattttcac cgtattctag cactttcaaa
    60541 atctgttgat tggtcaaacc tgattttttt aacttataga tttcatagtt tgtgattttc
    60601 ataaataact ccatttcttt ttctactcat ctatctattc gtaaaagttc gtaaaaaatc
    60661 aaaaaacatc cgactatttt gtcagatgtt ggaatcttta attttcgttt ttaagaattt
    60721 cttctaattt acgatagtct tggacactat cgatctcata aatgctattg ccttctaatt
    60781 cttcaacata gacatctagc tctttgatat tatccttaac catattgtcc caatagagat
    60841 caacaaattc accacttaca taagccttgt cgataaagct gacaatcttt tctgcagttg
    60901 gagcatccca gaaggataca ccactaagga tgcgacctgc cttgctatca acaataatgt
    60961 cttgaacctt gtagtcatct ccataaacca agaaccattc gttggtacaa tcttcacgat
    61021 aaacactaaa ataagtcgaa cgtgtcaaat cattgcggaa catattttta aagagataat
    61081 tgtcagcatc aataacatag ctgttggcca attcttcttt tacaagatag agagagtaaa
    61141 agttattgta gtcagcgtat ttatcattga aaacgagacg aacaccgtat ttctctttca
    61201 agtaatcgaa ttgttcttta agataaccaa caatgatgat gatgtcattg attccttttt
    61261 ctttgagaaa ctcaatttgg tactcaatca aaggtttttg attaacctga accaaggctt
    61321 taggggtatt ttcagtcata ggacgcaagc gagttcccaa tcccgctgct aagataatgg
    61381 ctttcacacg aatctccttt attctttaat aataatataa actccagcaa tgacgacaag
    61441 tgacgtcata atggtcagca tatctagcgg tgcacccaag aaaacaactg caaacaagac
    61501 cgtccatact acatagctca cgttcaagcc tgtagccttg gctggttgca agcgattgat
    61561 agcgatataa taagccaagt aggaaatcat atcaaaggct gcaaaaacaa tcatgagacc
    61621 tagcaattgt ccattggcta ctgcagtaaa tgactgatga gagaagagca caatcacaag
    61681 ataggacaag aacgaagtta cttgacggat taagagggct tcgatttcac tcaattcact
    61741 ttccatggca aaagagctaa gaacactctc acttccccat gcaatagcac aaaccaaagc
    61801 acaaagaatc ccaatgtaga aagaattaac ctgttcaacc ttataggtct gagcaataat
    61861 ccctccaata atcaagacaa tcccaaatac agtatttttc gaaatcttgt gcttcaaaaa
    61921 gaagaaagcc aatagaactg aaatcgcagg gtaaatagcc gatacagatg aagctaaaga
    61981 acttccgata tacttaactg cataaagatt ggcctgcata ccgatagggc ctgctagcaa
    62041 ggctccgatg ataacactga cattgcgaat atttaagaaa attgagaggc gaactttccc
    62101 ttcttttacc aagagaaaag ctagtaagat aaagatgctc aaaaaatcat gagttgcagc
    62161 caccacaaag ggtgacaaat ctgtaaaaat cgaaaagata taagcactaa ccgttagacc
    62221 caagccccag aaaatacctg agagaaggcc aaaaggaact ccgtttttac ttttcatctg
    62281 atcctccata agaagccaaa cctttaacag ctctttggta acgattcaca ccatagtcac
    62341 caaaatctgc accttgctct tccttatata cagtccatag actccaaata gtatcttgta
    62401 aaattttata aatagcaatc ttttcatgag aaaccggtgt ttggtcactc tcatagtgag
    62461 ataagaaagt ttcttcctct tgggaagtga attcagactc taaaaagagg gcagccaaat
    62521 cccacattgg atcattcatt gatgaatatt cccagtcaat caaataaagt cgtccttgag
    62581 gtgattcgat aaagttttca ggcaccaaat cgatatgaca agattttctg tcaacaccta
    62641 agtcagccag tcttttctct aaggagaaga ctgcatttct aacagattca tagttggcat
    62701 aaggaatttg ttcttcaatc aaggattcgt attttttgat ttcttcaaaa ggagcaaatt
    62761 ctcctcttaa ttccttagca gacgtatgaa tagtttgtaa tattggagca attttgtcga
    62821 acttggtctt gattgacgtt gaatcaagcg taatcgcaga tccgatatac tcatttactt
    62881 tgataccagc ttcaatatca aaaagataat tttttacatc taagcctaaa tcctttagta
    62941 gttcaagatt gtacttttca tcttgtcgat tgataagctt ttctgtccct ttaccaaaga
    63001 atttaacaat gtattgctta tttgttgttt tggccaaata gttttgattg gtcattccac
    63061 ccagttgttc aacactgagg acttcctctt cttgactaag taaggaagaa attttttctt
    63121 taatgatttt ctccacaatt aacctccagt acttatactt cccacttaaa cactgtttta
    63181 aaggctgtgt ttaaatcggt tgcaaagaca cgatgaatat ctttaatttc tcttacaggt
    63241 tcttctagat aaaggatatt tttaagacga ttggcaaatt tcttgacttc catcatttgg
    63301 atagcatttt caaaatcaat gcgaccagaa cgagatgacc caaccaagag caagcccttt
    63361 tctaaggcat cgcgagtatt gagattgact ttatattcgc taactcccat catgagaatc
    63421 gttccctgag gacgaatgta gcgaatcaag tcattaatag ctggtccagt accatcacca
    63481 ccacaacatt caaaagcatg gtcaaaggcc aaatcttcag gaatattatc cgtaatatag
    63541 cattctttgg caaatgagaa gagttccaac ttttcccaat gacgaccaat aaccacaatc
    63601 tctgcttctg gcaaagtata gttgataata ttggcaacca caaaagctaa acttccatct
    63661 ccaataacgg cgatccgctc ccgcttgcta tgagcaagag tcaatagacg attcatagcg
    63721 tgcatgccca cactgacaaa ctctgtaatg gctgcaaccg tatcttcaat agcatcataa
    63781 gccaccacac gatctttagg gagagaaaca aactctctca taaagccatc aaatccacta
    63841 gacaagaaat gggtccctgt catgtagttt tcatagaatt cttcatcact ctgcatagga
    63901 gactgattgg gaatcatgac aactttttga ccaacctcgt aggttccggt cgggtcagaa
    63961 atgacggttc cacatgactc gtgaatcatt gccattggaa gctttttatt caaaatcttg
    64021 ggatcacgtt ttccctgata gtaacgctga tccgcatgac agacagccat gtagttggga
    64081 cggataagga tatgattctc ttggtcaata gcctcttcct gatatttgac attgataaac
    64141 ttaggcttag ttagttgata aatttgatta atcattttac tagtctttct caatcatact
    64201 ttttgcaatc ttcaaatctg ttacggttgt aatcttcaga tttgagtatt cacctttggc
    64261 caaagccaca tcttttcctt tgatcacaaa gattttacat gcatctgtca agatttcctt
    64321 ctcttcatca gaaagagatc cataaaggtc catgaagtcc ttgcaacgga atgtttgagg
    64381 tgtttgtcct tgataaaggt gagcacgatt tggaatatct gtaataaatt gaccattggt
    64441 actttcaacg atagtatcaa ccgcttctac cactgtgtcc actgcgtcat gattttgggc
    64501 aagttggata ttgtcctgaa tcatgcgaag tgtaataaat ggacgaacag aatcgtgggt
    64561 aacaacgata tcctctggag taagcggacg ataagcatca atggcttcaa tgatgttctt
    64621 aatacttgta ttgcggtcag caccaccctt tgtaatgatg atacgttcct tataaagagg
    64681 aagatattta tctacaagat cttctgcatg agaaacccag tctccatgaa caccaactac
    64741 aattttttca atacttggct ccaagacaaa tttttcaatt gtatgaatca aaataggtcg
    64801 atcacctagc tctaaaaatt gttttggcaa gttactgatc cccatgcgtg tgccagttcc
    64861 accggcaaga attcctgcat aaatcatcta ttttctcctt tgtcttacta aaaaaactta
    64921 ttctctctat tataccacaa tctagtccta tcataaaaga aatactggtc ctccccttct
    64981 agaataggag aattatgcag ttctattatt caaagaaact agtccatttc tagtaatgac
    65041 taggaattct gtagcaatta ctaataaatg gctgcgaaat tttagagtcc ttcagaataa
    65101 tatactttcc ttaaagaaaa cttaaaactt aagatttgaa acttcaaagt gattacttga
    65161 cttactgccc ttcatttctt attagctaac tttatgatat aatgaaaaac gaggtaaatt
    65221 gaatgaaaag tataaaatta aatgctctat cttacatggg aattcgtgtc ttgaatatta
    65281 tttttcccat cctaactgga acctatgtcg cgcgtgtctt ggaccgaact gactatggtt
    65341 acttcaactc agtcgacact attttgtcat ttttcttgcc ctttgcaact tatggtgtct
    65401 ataactacgg tttaagggct atcagtaatg tcaaggataa caaaaaagat cttaacagaa
    65461 ccttttctag tcttttttat ttgtgcatcg cttgtacgat tttgaccact gctgtctata
    65521 tcctagccta tcctctcttc tttactgata atccaatcat caaaaaggtc taccttgtta
    65581 tggggattca actcattgcc cagatttttt caatcgaatg ggtcaatgaa gctctggaaa
    65641 attacagttt tctcttttac aaaactgcct tcatccgtat cctgatgctg gtctctattt
    65701 tcttatttgt taaaaatgaa cacgatattg ttgtctatac acttgtgatg agtttatcga
    65761 cgctgattaa ctacctgatt agttattttt ggattaaaag agacatcaaa cttgttaaga
    65821 ttcacctaag tgattttaaa ccactctttc tccctctgac agccatgtta gtctttgcca
    65881 atgccaatat gctcttcact tttttagatc gcctcttcct cgttaaaaca gggattgatg
    65941 tcaacgttag ttactatacc atagctcagc gaattgtgac cgttatagct ggggttgtaa
    66001 caggtgcaat tggagtgagt gtgcctcgtc tcagttacta tctggggaaa ggagacaaag
    66061 aagcctatgt ttctctggtt aatagaggta gtcgaatctt taacttcttt atcattccac
    66121 tgagttttgg actcatggtt ttaggaccaa atgccatcct actttacggt agtgaaaaat
    66181 atatcggagg cggcatcttg acctctctct tcgcttttcg tacgattatc ctggccttag
    66241 ataccattct tggttcccaa attctcttta ccaatggcta tgaaaaacgt atcacagttt
    66301 atacagtctt tgctgggcta ctcaatttgg gcttgaatag tctcctcttt ttcaaccgta
    66361 tcgtggctcc cgaatactac ttactgacaa ctatgttatc agaggcttct ctacttgttt
    66421 tctatatcat tttcatccat agaaaacaac tcatccactt gggacatatc tttagctata
    66481 ctgttcgata ctctctcttt tcactttcct ttgtagcaat ttatttcctg attaatttcg
    66541 tgtatcctgt ggatatggtc attaatttgc catttttgat taatactggt ttgattatct
    66601 tgctatcagc tatctcttat attagtctac ttgtcttcac aaaagatagc attttctatg
    66661 aatttttaaa ccatgttcta gccttaaaaa ataaatttaa aaaatcatag gagtttaaaa
    66721 tgaaacaact aaccgttgaa gatgccaaac aaattgaatt agaaattttg gattatattg
    66781 atactctctg taaaaagcac aatatcaact atattattaa ctacggtact ctgattgggg
    66841 cggttcgaca tgagggcttt atcccttggg acgacgatat tgatttatcc atgcctagag
    66901 aagactacca acgatttatt aacatttttc aaaaggaaaa aagcaagtat gaactcctat
    66961 ccttagaaac tgataagaac tactttaaca actttatcaa gataaccgac agtacgacta
    67021 aaattattga tactcgaaat acaaaaacct atgagtctgg tatctttatc gatattttcc
    67081 ctatggatcg ctttgatgat cctaaggtca ttgatacttg ttataaactg gaaagcttca
    67141 aactgctgtc tttcagtaaa cataaaaata ttgtctataa ggatagcctt ttaaaagatt
    67201 ggatacgaac agccttttgg ttgctccttc gaccagtttc tcctcgttat tttgcaaata
    67261 aaatcgagaa agaaattcaa aaatatagtc gtgaaaatgg gcagtatatg gcttttatcc
    67321 cttcaaaatt taaggaaaag gaagtcttcc caagtggtac ctttgataaa acaatcgatt
    67381 taccctttga gaatttaagc cttcctgcac ctgaaaaatt tgatactatt ttgacacaat
    67441 tttatggaga ttatatgacc ctaccaccag aagaaaaacg cttctacagt catgaatttc
    67501 acgcttataa attggaggat taggatgcaa tatttagaaa aaaaagaaat taaagaaatt
    67561 caactagccc tgctggacta tattgatgag acttgtaaga aacatgatat tccttatttt
    67621 ctcagttatg gaaccatgct tggagccatc cgccacaaag gtatgattcc ttgggatgat
    67681 gatattgata tttcccttta tcgtgaggat tatgagcgtt tactgaagat tattgaagaa
    67741 gaaaatcacc ctcgctacaa ggttctttcc tacgatacat cttcttggta cttccataat
    67801 ttcgcatcga ttttggacac ttctactgtt atagaagacc atgttaagta caagcgtcat
    67861 gataccagcc ttttcatcga tgtcttccca attgatcgat ttacagactt gagcattgtc
    67921 gacaagagct ataagtatgt ggctcttcgt caactagctt atatcaaaaa atcacgagca
    67981 gttcacggtg atagcaaact aaaagatttt cttagattat gtagctggta cgctctccga
    68041 tttgtcaatc ctcgctactt ttacaagaaa attgatcaac tagtcaaaaa tgctgtaacc
    68101 aacattcctc aatatgaagg aggagttggg atcggtaagg aagggatgaa agaaatcttc
    68161 ccagttgata cctttaaaga actgatttta actgagtttg agggccgtat gttgcctgtt
    68221 cccaaaaaat atgaccaatt tttaacccag atgtatggcg attatatgac accaccatca
    68281 aaagaaatgc aagagtggta tagtcatagc attaaagctt atcgcaaaaa ctgattgagg
    68341 gggattatac aaactactaa gatagaggtt attcaaaaac ataattttag tagaaaatga
    68401 aata
//
LOCUS       DAVKLX010000004        63911 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKLX010000004 DAVKLX010000000
VERSION     DAVKLX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63911
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.004"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            1..954
                     /locus_tag="VW325_000273"
                     /pseudo
     CDS             1..954
                     /locus_tag="VW325_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            1350..2213
                     /locus_tag="VW325_000274"
     CDS             1350..2213
                     /locus_tag="VW325_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9733744.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIALFE
                     LRLQQYKKLID"
     gene            2499..2699
                     /locus_tag="VW325_000275"
     CDS             2499..2699
                     /locus_tag="VW325_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU9733745.1"
                     /translation="MLNLQFAETMELTEAELEIVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            2766..3431
                     /locus_tag="VW325_000276"
     CDS             2766..3431
                     /locus_tag="VW325_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9733746.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            3503..4729
                     /locus_tag="VW325_000277"
     CDS             3503..4729
                     /locus_tag="VW325_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9733747.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICMINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTTLYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(5082..5156)
                     /locus_tag="VW325_000278"
                     /pseudo
     CDS             complement(5082..5156)
                     /locus_tag="VW325_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            5227..5883
                     /locus_tag="VW325_000279"
     CDS             5227..5883
                     /locus_tag="VW325_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU9733748.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWHNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGDLLPNPEIFGLDFALVGMFIGI
                     FASQFQMMQRRISVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGMVLDGQ
                     "
     gene            5873..6082
                     /locus_tag="VW325_000280"
     CDS             5873..6082
                     /locus_tag="VW325_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9733749.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKYKGLPTIVERFLKFL
                     PVSIIFALILSRVVTGKVGNLPQIK"
     gene            6300..7130
                     /locus_tag="VW325_000281"
     CDS             6300..7130
                     /locus_tag="VW325_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9733750.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            7284..8138
                     /locus_tag="VW325_000282"
     CDS             7284..8138
                     /locus_tag="VW325_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9733751.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            8238..9611
                     /locus_tag="VW325_000283"
     CDS             8238..9611
                     /locus_tag="VW325_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9733752.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYHTHIELVE
                     ELIRSYE"
     gene            9604..10665
                     /locus_tag="VW325_000284"
     CDS             9604..10665
                     /locus_tag="VW325_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9733753.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            10667..11359
                     /locus_tag="VW325_000285"
     CDS             10667..11359
                     /locus_tag="VW325_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9733754.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAIPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(11388..11957)
                     /locus_tag="VW325_000286"
     CDS             complement(11388..11957)
                     /locus_tag="VW325_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9733755.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            12092..12706
                     /locus_tag="VW325_000287"
     CDS             12092..12706
                     /locus_tag="VW325_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9733756.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            12708..14354
                     /locus_tag="VW325_000288"
     CDS             12708..14354
                     /locus_tag="VW325_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9733757.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            14366..15652
                     /locus_tag="VW325_000289"
     CDS             14366..15652
                     /locus_tag="VW325_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9733758.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFAIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            15704..16237
                     /locus_tag="VW325_000290"
     CDS             15704..16237
                     /locus_tag="VW325_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9733759.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            16315..16785
                     /gene="nrdI"
                     /locus_tag="VW325_000291"
     CDS             16315..16785
                     /gene="nrdI"
                     /locus_tag="VW325_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9733760.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(17078..18340)
                     /locus_tag="VW325_000292"
     CDS             complement(17078..18340)
                     /locus_tag="VW325_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9733761.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(18650..19108)
                     /locus_tag="VW325_000293"
     CDS             complement(18650..19108)
                     /locus_tag="VW325_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9733762.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(19105..19545)
                     /locus_tag="VW325_000294"
     CDS             complement(19105..19545)
                     /locus_tag="VW325_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9733763.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            19901..21850
                     /gene="mutL"
                     /locus_tag="VW325_000295"
     CDS             19901..21850
                     /gene="mutL"
                     /locus_tag="VW325_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9733764.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(22175..22642)
                     /locus_tag="VW325_000296"
     CDS             complement(22175..22642)
                     /locus_tag="VW325_000296"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9733765.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(22643..23848)
                     /locus_tag="VW325_000297"
     CDS             complement(22643..23848)
                     /locus_tag="VW325_000297"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9733766.1"
                     /translation="MEYRKIQETLEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLIERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(23868..24503)
                     /gene="ribE"
                     /locus_tag="VW325_000298"
     CDS             complement(23868..24503)
                     /gene="ribE"
                     /locus_tag="VW325_000298"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9733767.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSINGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(24488..25588)
                     /gene="ribD"
                     /locus_tag="VW325_000299"
     CDS             complement(24488..25588)
                     /gene="ribD"
                     /locus_tag="VW325_000299"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9733768.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(25723..25837)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25994..26587
                     /gene="ruvA"
                     /locus_tag="VW325_000300"
     CDS             25994..26587
                     /gene="ruvA"
                     /locus_tag="VW325_000300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9733769.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            26597..27160
                     /locus_tag="VW325_000301"
     CDS             26597..27160
                     /locus_tag="VW325_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9733770.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     VLLQLQAEYGSFDAYLWSFVEGKIVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            27157..27834
                     /locus_tag="VW325_000302"
     CDS             27157..27834
                     /locus_tag="VW325_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9733771.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(27989..29020)
                     /locus_tag="VW325_000303"
     CDS             complement(27989..29020)
                     /locus_tag="VW325_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9733772.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLSHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIKTGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(29275..29958)
                     /locus_tag="VW325_000304"
     CDS             complement(29275..29958)
                     /locus_tag="VW325_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9733773.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGSDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(29970..30914)
                     /locus_tag="VW325_000305"
     CDS             complement(29970..30914)
                     /locus_tag="VW325_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9733774.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            31046..33877
                     /gene="uvrA"
                     /locus_tag="VW325_000306"
     CDS             31046..33877
                     /gene="uvrA"
                     /locus_tag="VW325_000306"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9733775.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            33870..34931
                     /locus_tag="VW325_000307"
     CDS             33870..34931
                     /locus_tag="VW325_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9733776.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            35063..35461
                     /gene="spx"
                     /locus_tag="VW325_000308"
     CDS             35063..35461
                     /gene="spx"
                     /locus_tag="VW325_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9733777.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            35527..36096
                     /locus_tag="VW325_000309"
     CDS             35527..36096
                     /locus_tag="VW325_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9733778.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            36183..36449
                     /locus_tag="VW325_000310"
     CDS             36183..36449
                     /locus_tag="VW325_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9733779.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            36453..36872
                     /gene="ruvX"
                     /locus_tag="VW325_000311"
     CDS             36453..36872
                     /gene="ruvX"
                     /locus_tag="VW325_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9733780.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            36888..37193
                     /locus_tag="VW325_000312"
     CDS             36888..37193
                     /locus_tag="VW325_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9733781.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            37451..38701
                     /locus_tag="VW325_000313"
     CDS             37451..38701
                     /locus_tag="VW325_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9733782.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            38698..39240
                     /locus_tag="VW325_000314"
     CDS             38698..39240
                     /locus_tag="VW325_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9733783.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            39386..40918
                     /gene="cls"
                     /locus_tag="VW325_000315"
     CDS             39386..40918
                     /gene="cls"
                     /locus_tag="VW325_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9733784.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            41176..42714
                     /locus_tag="VW325_000316"
     CDS             41176..42714
                     /locus_tag="VW325_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9733785.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWGLHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            42834..45041
                     /gene="nrdD"
                     /locus_tag="VW325_000317"
     CDS             42834..45041
                     /gene="nrdD"
                     /locus_tag="VW325_000317"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9733786.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            45053..45193
                     /locus_tag="VW325_000318"
     CDS             45053..45193
                     /locus_tag="VW325_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733787.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            45237..45743
                     /locus_tag="VW325_000319"
     CDS             45237..45743
                     /locus_tag="VW325_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9733788.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            45736..46326
                     /gene="nrdG"
                     /locus_tag="VW325_000320"
     CDS             45736..46326
                     /gene="nrdG"
                     /locus_tag="VW325_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9733789.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            46323..46940
                     /locus_tag="VW325_000321"
     CDS             46323..46940
                     /locus_tag="VW325_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9733790.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            47206..47514
                     /gene="rpsJ"
                     /locus_tag="VW325_000322"
     CDS             47206..47514
                     /gene="rpsJ"
                     /locus_tag="VW325_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9733791.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            47731..48357
                     /gene="rplC"
                     /locus_tag="VW325_000323"
     CDS             47731..48357
                     /gene="rplC"
                     /locus_tag="VW325_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9733792.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            48382..49005
                     /gene="rplD"
                     /locus_tag="VW325_000324"
     CDS             48382..49005
                     /gene="rplD"
                     /locus_tag="VW325_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9733793.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            49005..49301
                     /locus_tag="VW325_000325"
     CDS             49005..49301
                     /locus_tag="VW325_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9733794.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            49319..50152
                     /gene="rplB"
                     /locus_tag="VW325_000326"
     CDS             49319..50152
                     /gene="rplB"
                     /locus_tag="VW325_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9733795.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            50256..50537
                     /gene="rpsS"
                     /locus_tag="VW325_000327"
     CDS             50256..50537
                     /gene="rpsS"
                     /locus_tag="VW325_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9733796.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            50549..50893
                     /gene="rplV"
                     /locus_tag="VW325_000328"
     CDS             50549..50893
                     /gene="rplV"
                     /locus_tag="VW325_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9733797.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            50906..51559
                     /gene="rpsC"
                     /locus_tag="VW325_000329"
     CDS             50906..51559
                     /gene="rpsC"
                     /locus_tag="VW325_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9733798.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            51563..51976
                     /gene="rplP"
                     /locus_tag="VW325_000330"
     CDS             51563..51976
                     /gene="rplP"
                     /locus_tag="VW325_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9733799.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            51986..52192
                     /locus_tag="VW325_000331"
     CDS             51986..52192
                     /locus_tag="VW325_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9733800.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            52217..52477
                     /gene="rpsQ"
                     /locus_tag="VW325_000332"
     CDS             52217..52477
                     /gene="rpsQ"
                     /locus_tag="VW325_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9733801.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            52503..52871
                     /gene="rplN"
                     /locus_tag="VW325_000333"
     CDS             52503..52871
                     /gene="rplN"
                     /locus_tag="VW325_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9733802.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            52949..53254
                     /gene="rplX"
                     /locus_tag="VW325_000334"
     CDS             52949..53254
                     /gene="rplX"
                     /locus_tag="VW325_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9733803.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            53278..53820
                     /gene="rplE"
                     /locus_tag="VW325_000335"
     CDS             53278..53820
                     /gene="rplE"
                     /locus_tag="VW325_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9733804.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            53838..54107
                     /gene="rpsN"
                     /locus_tag="VW325_000336"
     CDS             53838..54107
                     /gene="rpsN"
                     /locus_tag="VW325_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9733805.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            54392..54790
                     /gene="rpsH"
                     /locus_tag="VW325_000337"
     CDS             54392..54790
                     /gene="rpsH"
                     /locus_tag="VW325_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9733806.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            54982..55518
                     /gene="rplF"
                     /locus_tag="VW325_000338"
     CDS             54982..55518
                     /gene="rplF"
                     /locus_tag="VW325_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9733807.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            55602..55958
                     /gene="rplR"
                     /locus_tag="VW325_000339"
     CDS             55602..55958
                     /gene="rplR"
                     /locus_tag="VW325_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9733808.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            55976..56470
                     /gene="rpsE"
                     /locus_tag="VW325_000340"
     CDS             55976..56470
                     /gene="rpsE"
                     /locus_tag="VW325_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9733809.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            56442..56666
                     /gene="rpmD"
                     /locus_tag="VW325_000341"
     CDS             56442..56666
                     /gene="rpmD"
                     /locus_tag="VW325_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9733810.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            56811..57251
                     /gene="rplO"
                     /locus_tag="VW325_000342"
     CDS             56811..57251
                     /gene="rplO"
                     /locus_tag="VW325_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9733811.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            57264..58574
                     /gene="secY"
                     /locus_tag="VW325_000343"
     CDS             57264..58574
                     /gene="secY"
                     /locus_tag="VW325_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9733812.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            58725..59363
                     /locus_tag="VW325_000344"
     CDS             58725..59363
                     /locus_tag="VW325_000344"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9733813.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            59480..59698
                     /gene="infA"
                     /locus_tag="VW325_000345"
     CDS             59480..59698
                     /gene="infA"
                     /locus_tag="VW325_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9733814.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            59723..59839
                     /gene="rpmJ"
                     /locus_tag="VW325_000346"
     CDS             59723..59839
                     /gene="rpmJ"
                     /locus_tag="VW325_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9733815.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            59857..60222
                     /gene="rpsM"
                     /locus_tag="VW325_000347"
     CDS             59857..60222
                     /gene="rpsM"
                     /locus_tag="VW325_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9733816.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            60240..60623
                     /gene="rpsK"
                     /locus_tag="VW325_000348"
     CDS             60240..60623
                     /gene="rpsK"
                     /locus_tag="VW325_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9733817.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            60666..61601
                     /locus_tag="VW325_000349"
     CDS             60666..61601
                     /locus_tag="VW325_000349"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9733818.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            61613..61999
                     /gene="rplQ"
                     /locus_tag="VW325_000350"
     CDS             61613..61999
                     /gene="rplQ"
                     /locus_tag="VW325_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9733819.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            62264..62530
                     /locus_tag="VW325_000351"
     CDS             62264..62530
                     /locus_tag="VW325_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9733820.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            62540..63877
                     /locus_tag="VW325_000352"
     CDS             62540..63877
                     /locus_tag="VW325_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9733821.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
BASE COUNT        19123 a        11983 c        14003 g        18802 t
ORIGIN      
        1 attgattatt atattattgc gcttccgaca gattatgatg agaaaattgg tagttttaat
       61 acatatgaaa tcgaacaaac ggtatcgaag attctgaggg taaaacctaa tggaaagatt
      121 attttaaagt caacagttcc gttcggcttt tcaaacaaat taaaaaggct gtttgataca
      181 aaaaatatca tttttgtccc tgaatttttg agagaaggtt gttctatata tgataattta
      241 tatccaagtc gcatagttgt tggagatgag acagttgaag gaagaaaaat tgcagagttg
      301 ttcctttcga ttagtactca tagtactgcc aatattaaaa atgttatgtt agtttctcct
      361 actgaagcag aagcaattaa gcttttttct aacacattct tagctctccg tgttgctttt
      421 tttaatgaac tagattcttt tgctgagagg agaagtttaa atgctgaagt tgtaataaaa
      481 ggtgtttgtt tagatccaag aattggaaat ttttataata atctttcttt tggatttgga
      541 ggatattgtc ttcccaaaga tactaaacaa taaaaaaaag aatttataga aataaatgcc
      601 ccagtgatag aagcgattga tatttcaaac acaaatagaa aacagttcat agttaaacaa
      661 atattggaac gaaagccaaa gatagtagga atatataaat tggggatgaa atataattca
      721 gataattaca aagagtcagc tattttaagt ataattaatg aactattgat tgtcggtata
      781 aaaattttag tatatgaacc aaatttaaac gttagtatag ataacgtaat ttttgaaaag
      841 aattttgaac tatttacaaa acaaagtgat ctaattgttg caaatagatg ggataggggc
      901 cttgaagctt ataaagataa agtttacaca agggggatct ggataagaga ttaactattg
      961 atattaaaaa ggcaataatt tataaaattt gtacaaaaaa atgttaaatt ttcacatttt
     1021 catagtatac tgtttaatag aaagtaggtt ctttgtaacc gctccattat aaaatccttt
     1081 cactcttccg gtatactatt aggaaaaaca taactggagg atattttgag ctcacacctc
     1141 atcgttccag tggctgttcc ccaagctcgt gtactgccca aaaaagttag acattctatt
     1201 tagaacgagg attgagttct gtattgtaca aggctcggtc cttttagagt cagcttaagg
     1261 ctggcttttt tattttttct aactatcaga atattttgac aaatgaacac aaataatgat
     1321 ataatatgca aaagctagga ggtggtagga tgattgaaaa aatggaattg ggggaatttt
     1381 acaaggaatt acgtttggcg agaaagctca agcagtcaga tgtggcttgt gacgggctaa
     1441 cagcttctca gttgtccaag tttgagctag ggcagtccat gttgtctgct gataagctca
     1501 tattagccat ccaagggatc aatgtgacct ttgatgagtt tgggcacaag ctcaacaact
     1561 atcaagaatc tccacatatg cagattggta gaaaggttgt ggatcgcttt gctcatcaag
     1621 atatagctgg cttagagcaa ctgttggagg aagtcgagca agaacagatg gcagagactt
     1681 atcgtcgttt gaatgctatt gtaatcaaag atgccatcca ctcactagat aaaagctacc
     1741 cactagcaga ggaggatagc gagtttttga ccacctatct ctatgctatc gagtcttgga
     1801 cctggtttga actctatctc ttttgcaata ctctgccctt cttgagcaat caagatctga
     1861 tctttttatc aacctcctta ctcgaaaaat ccaaagaatt taaagagtta gtacacaatc
     1921 gattgtatat gaagcaagga ctcttaaata tcatctcaga gctcatggag cgcaaacttt
     1981 tttcttatat cccaatcttt gaagccgagc tggagagtat gctccgtcca tacgatgttt
     2041 ttgagaaagt gttgtggcag tttttaaaga agatgagcat tttccttcaa actaagggaa
     2101 gcaatcaaaa agagattgaa cactttatcc aatctctgca agtattagaa aatccacaat
     2161 taatagccct ttttgaattg cgtttgcagc aatacaaaaa gcttatcgat tagttagaaa
     2221 tggcaaaata ataataatct cagcaacgat tttcaataat gttaaaattt gtttcttcat
     2281 atttctccac tcctcattag tagtaacttt attataccag agaagtaatc aaattcacca
     2341 aaatcgcaaa tctgaaattt ctataagaaa aaatatcaaa tatgcgattt tctaaaataa
     2401 gccaattttc gtgttatact gtacttgtaa agcacttgaa gcaaatccta ggtcgcagaa
     2461 gtggtttaca aatgaagata attgaaggag tgtaaaagat gttaaattta caatttgcag
     2521 aaacaatgga attgacagaa gctgagttgg agatagttta tggaggggaa tttgggaata
     2581 atgctgttat cccagccggt gcttggggag ggttcgggac accttggtca atcactaatt
     2641 tctggaagaa aaattttaac gatagacctg actttgattc tgaccgccgt cgttattaat
     2701 atccaaagcc cttgcctttt cttgggtagg ggctgtcttt atttcatagt ataggaggaa
     2761 atagaatgaa aaagataatc tcacaccgct actttattat agtttttcta ctagttattg
     2821 ctgaccagaa gttcagtgtt ctagttttac gcagcgacct tgttactggg ctatctgact
     2881 ttgcctatta tctgtcggat atgatgttga attttcttgt ggttttattt gctcttattg
     2941 ctatgatttg gtcggggaaa tggcagaaaa tcaatagtag aaagtttaaa ggatcctatc
     3001 ttttctattc cttcctagct ctgcttgctt ttgttgtttg gaatttcgtt accttttttc
     3061 ttttcccacc tactcgaaat gaaatttctt atcaacatgc tgctccgact tttacagggg
     3121 ctacagcatt tttgatgtat tttttctatc ctgtgattgc aggtcccatt tttgaagaga
     3181 taatccatcg gggattggtg atgacagctc tggaaaaagg aaagaaatgg gggctagatg
     3241 tacttggctc tgctatttta tttggggtct cgcacattag taatcacggt tgggtcttga
     3301 cagactttgt cttctatatg ggtggaggtc ttatatttgc agtcttattt agaatgacaa
     3361 agtcaattta ttggcctatt ggactgcata tagtctacaa tggtattggt cagcttttga
     3421 tgttactgta attttggttg ttagtagtat cgcggtctat tcttggggaa agattcccat
     3481 ttgaacttaa gaaggagttg taatgaaagt atttcttcaa aatagagatt ttaggcaatt
     3541 aactatcaac cagtggattt caacgcttgg ggatacgatt ttttatctgg ccttcctgaa
     3601 ttatgtggca gatgcgtctt ttgccccttt ggcgatttta cttatcacga tttcagaaac
     3661 ccttccccaa gttctacaaa tctttctggg agttttggcg gattttcaac atcatcgtgt
     3721 cttgaaatac acagtcatta gttttacaaa atttttgctt tactctatag tgtccctatc
     3781 gctttcaggg cagtcttttt ccttgttgct agtaaccttt atttgtatga ttaacctctt
     3841 gtctgacaca ttgagttatt tttcaggcgc catgctcact ccgattttca ttagaattat
     3901 tgggcaagat catctggcag aagctattgg atttaaacag tcaactgtta gtttagtgaa
     3961 aacaatcagt aatatcctag gaggagtctt actaggtatt ctatccatcc agtttatttc
     4021 cttactgaac gctcttacct ttttaatcgc atttttaggt atcctcttca taaaaactga
     4081 cctcttgaaa gtagaaaaaa cgattagcta tcaagaagga ctctctgtaa aatccttttg
     4141 ccagcatttg ctccaatcat cgaaattgat atggaatatg aataaggtgc tcttggtttt
     4201 gtttattatc tctactagtc aagcagtgat aaatgttaca gttcctgtct ctactctatt
     4261 tttgaggaat caaccctttt tgaatttaca aacaggtcaa tctcttgcct tgctatccac
     4321 ttttgaactg tcagccctga ttgtcggaag ccttgtaagt ggctatctgc aacataccat
     4381 ttccataaaa acaactctct atgcctcact tgtcatccag ttgcttcttc tagtaggatt
     4441 tgccacagtt cgttttgact ggattctcat ctttagtacc ttggatgcct ttttcgcagg
     4501 tgttctctct cctagattgc aagaactcgt ctttaaacaa atacctgagg agtcaatggg
     4561 agcagttcaa tcttctatca gtgccattac ggttgtttta cctagcttat ttacaatatc
     4621 tttggtaacc attgctacta gctttggaac tctggcagtt agctttgttt tattgctatt
     4681 tcttctagtt gcctttgtta tgcttctgaa tattcgtgaa agtatttagt ggagaatttc
     4741 tataatatat gtttgagact ggattgccag tctttttatg atggtcaaaa tcaacagact
     4801 ttttctcttt tctgaatgtg ttatagtaga ctgaatctaa aatagtacga aacaattgct
     4861 aaaacattta tagaaattaa ttttactttc ccaatcgatt tgttctcatc ttatttcaat
     4921 ctgctatata atagtccatg ctgtggctgg attcttttca gcctacttat actcttcgaa
     4981 actctcttca aaccacgtca gcttcacctt gccgtatatg tgttactgac ttcgtcagtt
     5041 ctatccacaa cctcaaaaca gtgttttgag ctgacttcat cagttctatc tgcaacctca
     5101 aaacagtgtt ttgagcaacc tgcggctagt ttcctagttt gctctttgat tttcattgag
     5161 tattattaag acaatatgag gagaaagatg aaagaaaaag gattttagga gggggcgcag
     5221 gcagccatgc caacggctct tggctatgtc agtatcggcc tggcctgtgg aattatcggt
     5281 gcgccctatg tgacacctgt tgagatgggc ttgatgagtc tctttgttta tgctgggagt
     5341 gcccagtttg ccatgttggc actgattgtg gttcaagctc ctgtggcagc tattgctatg
     5401 acggtttttc taatcaattt gcgtctcttt ttgttgagct tacacgcatc gacttatttc
     5461 cgtcatacca gtctttggca caatatcggt atgtctagta tcttgacaga tgagacctat
     5521 ggcgttttga tgggcgaatt agcccataca gataaggtca atccgatgtg gatgcacggg
     5581 aacaatctca acagctatgt ggcttggttt gtgggaacag tggtcggaac ggctctaggt
     5641 gacctgttgc caaatccaga aatctttggt ttagattttg ccctggttgg gatgtttatc
     5701 gggatttttg cttcgcaatt ccagatgatg caaagacgga tttctgtccg caatctgctc
     5761 attatcttag cagttgttgc agtgtccttc tttttacttt tgacagtggt gtctcagtca
     5821 ctagctgttc tgtttgcgac gctacttggt tgtagcatgg ggatggtttt agatggtcag
     5881 taagtatctt ttattagcag ttattttctc tggtcttgtg acctggattc cccgtatgat
     5941 tcccttcatc ttggtcaagt ataagggcct gcctacgatt gttgagcgtt ttttgaagtt
     6001 tttgcctgtt tccattatct ttgccttgat tctttcaaga gtagtgacag gtaaggttgg
     6061 gaaccttcct caaatcaaat gactggactt cttagcagtc tttccaacag cttgggtagc
     6121 ttttcgctac cgcaatctag tcggtacggt tctctttgga gtagtcttga ttgccgtctt
     6181 gcgtttggtc ttttaaataa gtcataaaga aaacttatca cagagataga tatcatataa
     6241 tggcgtaatt actttttttc tgataagatt ataaggtatt ctattttgga ggaaatgaca
     6301 tgaaaaaaat cgttaaatac tcatctcttg cagcccttgc tcttgttgct gcaggtgtgc
     6361 ttgcggcttg ctcaggtggt gctaagaaag aaggacaagc agctagcaag aaagaaatca
     6421 tcgttgcaac caatggatca ccaaggccat ttatctatga agaaaatggc gaattgactg
     6481 gttacgagat tgaagtcgtt cgcgctatct ttaaagattc tgacaaatat gatgtcaagt
     6541 ttgaaaagac agaatggtca ggtgtctttg ctggtcttga cgctgaccgt tacaatatgg
     6601 ctgtcaacaa tcttagctac actaaagaac gtgcggagaa atacctctat gccgcaccaa
     6661 ttgcccaaaa tcctaatgtc cttgtcgtga agaaagatga ctctagtatc aagtctctcg
     6721 atgatatcgg tggaaaatcg acggaagtcg ttcaagccac tacatcagct aagcagttag
     6781 aagcatacaa cgctgaacac acggacaacc caactatcct taactatact aaggcagatt
     6841 tccaacaaat catggtacgt ttgagcgatg gacaatttga ctataagatt tttgataaaa
     6901 tcggtgttga aacagtgatc aagaaccaag gtttggacaa cttgaaagtt atcgaacttc
     6961 caagcgacca acaaccgtac gtttacccac ttcttgctca gggtcaagat gagttgaaat
     7021 cgtttgtaga caaacgcatc aaagaacttt ataaagatgg aactcttgaa aaattgtcta
     7081 aacaattctt cggagacact tatctaccgg cagaagctga tattaaataa tttcttgaaa
     7141 tcatccattc tgagtaagat gggtggtttc tcagttttta gggatatagt tttaaactat
     7201 atatctgact atctaataac aatttgtgaa atcaaacaaa ttgtgagata ctagtacggt
     7261 attattttta aggagaaaga atcatgaaaa tcaaaaaatg gcttggtcta gcagcccttg
     7321 ctacagtcgc aggtttggct cttgcagctt gcggaaactc agaaaagaaa gcagacaatg
     7381 caacaactat caaaatcgca actgttaacc gtagcggttc tgaagaaaaa cgttgggaca
     7441 aaatccaaga attggttaaa aaagacggaa ttaccttgga atttacagag ttcacagact
     7501 actcacaacc aaacaaagca actgctgatg gcgaagtaga tttgaacgct ttccaacact
     7561 ataacttctt gaataactgg aacaaagaaa acggaaaaga tcttgtagcg attgcagata
     7621 cttacatctc tccaatccgc ctttactcag gtttgaatgg aagtgccaac aagtacacta
     7681 aagtagaaga catcccagca aacggagaaa tcgctgtacc aaatgacgct acaaacgaaa
     7741 gccgtgcgct ttacttgctt caatcagctg gcttgattaa attggatgtt tctggaactg
     7801 ctcttgcaac agttgccaac atcaaagaaa atccaaagaa cttgaaaatc actgaattgg
     7861 acgctagcca aacagctcgt tcattgtcat cagttgacgc tgccgttgta aacaatacct
     7921 tcgttacaga agcaaaattg gactacaaga aagcactttt caaagaacaa gctgatgaaa
     7981 actcaaaaca atggtacaac atcattgttg cgaaaaaaga ttgggaaaca tcacctaagg
     8041 ctgatgctat caagaaagta atcgcagctt accacacaga tgacgtgaaa aaagttatcg
     8101 aagaatcatc agatggtttg gatcaaccag tttggtaata agaaacaggg aggtgggaga
     8161 gaaaattcca cctcttgctt ttgtatagag tatagattgt aaagaagact attcgttcat
     8221 agaaaggtag agagaatatg gtttttccta gcgaacaaga acagattgaa aaatttgaaa
     8281 aggatcatgt agtccaacat tattttgagg ttttgcgtac cttgatttct aagaaatcag
     8341 tctttgccca gcaggttgga ctcaaggaag tcgcaaatta tctgggtgag attttcaagc
     8401 gtgttggagc tgaagtggag attgatgaga gctatacagc gccctttgtc atggcacatt
     8461 tcaagagttc gcgtccagat gccaagacct tgattttcta taaccactat gacactgtgc
     8521 cagcggatgg ggatcaggtc tggacagagg atccttttac gctttcggtc cgcaatggct
     8581 tcatgtatgg gcgtggggtt gatgacgaca agggtcatat cacagctcgc ttgagtgctt
     8641 tgagaaaata tatgcagcac catgatgatt tacctgtcaa tatcagcttt atcatggagg
     8701 gagcggagga atcggcttca acagacctag ataagtattt ggaaaagcat gcagacaaac
     8761 tccgtggggc ggatttgttg gtctgggaac aagggaccaa aaatgccttg gagcagctgg
     8821 aaatttctgg tggcaataag gggattgtga cctttgatgc caaggtaaaa agcgctgatg
     8881 tggatatcca ctcgagttat ggtggtgttg tggaatcagc tccttggtat ctcctccaag
     8941 ccttacagtc tcttcgtgct gcggatggcc gtatcttggt tgaaggcttg tacgaagaag
     9001 tacaagagcc caatgaacga gaaatggcct tgctagaaac ttatggtcaa cgaaacccag
     9061 aggaagttag tcggatttat ggattggagt tgcctctctt acaggaggag cggatggcct
     9121 ttctaaaacg tttctttttc gagccagcgc ttaatatcga aggaatccag tctggttatc
     9181 aaggtcaggg tgttaagact attttacctg cagaagccag tgccaagcta gaggttcgtc
     9241 tggttccggg cctagaaccg catgatgttc tggaaaaaat tcggaaacag ctagacaaaa
     9301 atggctttga taaggtagaa ttatactata ccttgggaga gatgagctat cgaagcgata
     9361 tgagcgcacc agccattctc aatgtgatcg agttggccaa gaaattctat ccacagggcg
     9421 tttcagtctt gccgacgaca gcggggacag gacctatgca tacggtcttt gatgccctag
     9481 aggtaccaat ggttgcattc ggtctaggaa atgccaatag ccgagaccac ggtggagatg
     9541 aaaatgtgcg aatcgctgat tatcacaccc atatcgaatt agtagaggag ctgattagaa
     9601 gctatgagta gagatattat caagttagat cagatcgatg tgacttttca ccaaaagaag
     9661 agaaccatta cagcggttaa ggatgtgacc attcacatcc aagaagggga tatctacgga
     9721 atcgttggat attctggagc aggaaaatca acccttgtac gggtgattaa tctcttgcaa
     9781 aaaccatctg cagggaaaat taccattgac gacgatgtga tttttgacgg caaggtgacc
     9841 ttgacggcag agcagttgcg tcgtaaacgt caagatatcg gaatgatttt ccagcatttt
     9901 aacctgatga gccaaaagac agcagaggag aatgtagcct ttgcccttaa acactctgaa
     9961 ctcagcaagg aagaaaagaa ggctaaagta gctaagttgt tggacttggt tggtttggca
    10021 gatcgtgctg aaaactaccc ttcacaacta tctggagggc aaaaacagcg tgtggcaatt
    10081 gcgcgtgcct tggccaatga tccaaaaatc ttgatttcag acgagtcaac ttctgccctt
    10141 gatccgaaga caaccaagca gattttggcc ttgttgcaag atttgaacca aaaattaggc
    10201 ttgactgttg tcttgattac gcatgaaatg cagattgtca aagacattgc caaccgtgtg
    10261 gcggttatgc aggatgggca tttgattgaa gagggtagtg tgcttgaaat cttctcagac
    10321 cctaaacaac ctttgactca agactttatc tcaacagcta caggtattga cgaagccatg
    10381 gtcaaaatcg agaagcaaga aatcgtggaa cacttgtctg aaaacagtct cttggtgcaa
    10441 ctcaagtacg ctggatcttc aacagacgag ccacttttga atgaattgta caagcattat
    10501 caagtaatgg ctaatattct ctatgggaat atcgaaatcc tcgatggtac tcctgttgga
    10561 gaattggtgg tggtcttgtc aggtgaaaaa gcagcgttgg caggtgccca agaagccatt
    10621 cgtcaagcag gcgtacagtt aaaagtattg aagggaggac agtaagatgg aatcattgat
    10681 tcaaacctat ttgccaaatg tctataagat gggttggtct ggtcaggcag gctggggaac
    10741 agctatctac ctaaccctct atatgacagt tctttccttc attatcggag gcttcttggg
    10801 gctagtggca ggtctctttc tcgtcttgac agcgccaggt ggtgtcttgg agaataaagt
    10861 cgtattctgg attttagaca aaattacctc aatttttcgt gcgattccct ttatcatcct
    10921 cttggcaatc ttgtcaccac tttctcactt gattgttaaa acaagtatcg ggccaaatgc
    10981 agcccttgtc ccactttctt ttgcagtctt tgccttcttt gcccgtcagg tgcaggttgt
    11041 cttggctgaa ttggatggcg gtgtcattga ggcggctcaa gcgagcggag cgactttctg
    11101 ggacatcgtg ggtgtttacc tatcagaagg tcttccagat ttgatccgtg tgacgactgt
    11161 gaccttgatt tcccttgttg gggaaacagc tatggccggt gcggttggag ctggtggtat
    11221 cggtaacgta gccatcgctt atggatttaa ccgctacaat cacgatgtga ccatcttggc
    11281 aaccattatt atcattttga ttatctttgc aatccaattc ttgggagatt tcttgactaa
    11341 gaaattgagt cataaataaa aagagccgcg tggctctttt taactgatca gattttctgt
    11401 gcaaattttt tactcaaggc ttgtccaatc aaggcaccca ctagggctcc gatgacaata
    11461 cttgcgataa atagaaggac agttccaggg tttggagcga ccatgatacg gtcgatatat
    11521 tcttgggatt ttcctcttgc cagaagagta gccatatagg ctttgggcgc aatccacata
    11581 agcaagattg gtcctgttgt actaaaggcg aaaataatga aagaaaggaa gttctttgtt
    11641 ttgtccttgt attttcctaa atgagctact tcatctgcta ggaggccaca gataattcca
    11701 ggaaggaagg aaccagcacc gtgtttagtt cccaagaaaa agagggcaat gacaaggccg
    11761 atagtggtaa tggctccaaa gcgcggaact tttgcgacta ggatcatata gacgctaccg
    11821 ccgacaaggg cagtaaaggc aggcgcataa aacatgtttc cagtttggtc aaagagattg
    11881 cccaaaagga caccaatccc catgcagagg aagtaaagaa ctgcaaaaag cagtgtagtt
    11941 aagatagatt tcttcatgaa ttgtctcctg ataatttttt cacaattctc attgtaccac
    12001 ggtttggtgg gattgtaaat ggatactaga agtttttgaa aacgcttgaa atatgatata
    12061 atggtttcat agttattttt aggggaatat catgggtaga tttttagact ttgtctttaa
    12121 tcgtttcttt ttagggatga ttgcgacagc cttcttttgg ctattaactt tagcaggagg
    12181 gattatcctt ggtctagcgc cggctagtgc caccttgatg agcttatatg cagaacatgg
    12241 ttatagcttt cgggaataca gtttgaagga ggcttggtct ctttacaagc aaaattttgt
    12301 ctcaagcaac ctgattttct atagcttttt aggtgtgggt ctagttttga cctatggttt
    12361 gtatctcttg gtgcaattgc ctcatcagac cattgttcat ttgattgcga cccttttgaa
    12421 tgtcctagta gttgccctga tctttttggc ttatacagta tctttaaaat tacaagttta
    12481 ttttgccttg tcctatcgaa atagtctcaa attatccttg attggcatct ttatgagtct
    12541 agcagctgtg gccaagattc tccttgggac tgtgctactt gtagcaattg gttactatat
    12601 gcctgccctg ctattttttg taggaattgg gatgtggcat ttctttatca gtgatatgtt
    12661 ggaacctgtc tatgaaatca tccatgaaaa attggcgaca aaatagaatg aagcagtttt
    12721 ggctacatac gcttctaaga acctatagtt cagtgatgat cattatcatt gcgagttttg
    12781 caatcttact ctcttacgct gactgggatt cacgtgaaaa ggaagcccag agagtagccc
    12841 agcgtgtaac tgctagaaca gtgagtgaaa ttgaatatta ccatagagag tcaacccaga
    12901 tagctcaggc tttagttgaa aaccaagctc gtattgaggg aatctataaa tactttagtc
    12961 ttagcatgcc agactatttt tactggcaat tagagcggaa agcttcgcct tatatatcag
    13021 tctctctgta tgaaaatgtt gatgacctct atgttcgaaa tgattttgta actggagtgg
    13081 ccattgcttt tcaagattac aaggaagtct atgtttctac taaagacaaa cgtagtggag
    13141 aaaaaatcag ggctgaggat ttcaaaccag caggaaatag ttttgccatt ccggtgtcag
    13201 atccagtgtc agatcaagat ttaggagtga tttacatctc cttggatcct gctgttttat
    13261 accatgccat tgataatact agaggtcata ctccgatggc agtaacagtg acctcacctt
    13321 ttgatacgga gatttttcat attggtgaga cagttgataa ggagagtgaa aattggctag
    13381 ttggcttaac ttctcatgga tatcaggttc aggtggcagt tcctaaaaac tttgttttac
    13441 aaggaacagt gactagctct gctttgattg tgggtttgag ccttctcttt attgtcattc
    13501 tttatctgac tttgaggcag acttttgcta attaccaaaa gcaggtagtg gatttagtag
    13561 aatccattca agtcattgct caaggcgaag aggggcgtcg gattgacatt tccgagaaag
    13621 atcaggaatt actcctaatc gcggagacga ccaatgatat gttggatcga ttggaaaaga
    13681 atatccatga tatttaccag ttagagctta gtcaaaaaga tgccaatatg cgagccttgc
    13741 aggcgcaaat caatcctcat tttatgtata atacgctgga gttcttgcgc atgtatgcag
    13801 ttatgcagag tcaagatgag ttggcagata tcatttatga attcagtagt ctcttgcgta
    13861 acaatatttc cgacgaaaga gagaccctcc tcaaacagga attagaattt tgccgtaaat
    13921 acagctatct ctgcatggtt cgctatccca agtccattgc ctatggtttc aagatagatc
    13981 cagagttaga gaatatgaag attcccaagt ttaccttgca accgctggta gaaaactatt
    14041 tcgcgcatgg tgttgaccac aggcggacag ataatgtgat tagcatcaag gctcttaaac
    14101 aggatggttt tgtggaaatt ttggtggtcg ataatggtag aggaatgtcg gctgaaaagt
    14161 tggcaaatat ccgagaaaaa ttaagtcaga gatattttga acaccaagcc agctacagtg
    14221 atcaaaggca gtctatcggg attgtcaatg tacacgagcg ttttgtgctc tattttggag
    14281 accgctatgc cattacgata gagtctgcag agcaagccgg tgttcagtat cgtattacaa
    14341 ttcaagatga gtagaaaggg agaaaatgta taaagtatta ttagtagatg atgagtacat
    14401 ggtgacagaa ggtctgaagc gtttgattcc ctttgataag tgggatatgg aggtcgtcgc
    14461 aacagccagt catgccgatg aagctctaga atatgttcag gaaaatcctg tcgatgtcat
    14521 catttccgat gtcaatatgc cagacaaaac agggcttgat atgattcggg agatgaaaga
    14581 gatcttacca gatgctgcct atatcctgct ctcaggttat caggagtttg attatgtaaa
    14641 aagagcaatg aaccttagtg tggtggacta tttggtcaag cctgttgata aggtagagct
    14701 gggaaatctg ctggagaaga ttgcaggtca gctcggcgag agagggaaga aaagtcagac
    14761 tcttagtcaa gaattagacg aggctggatt tgttagttat ttaggggata aggagaattg
    14821 gtggataggt ctatccaagg aaaaacaagg ttccttcgcc attccctact atgtcttggg
    14881 ccaagactgg cagattttca tttctgacca acccctagat ggtttagtcg ttacaccttt
    14941 tgaagctcct tatcaagaac actttgaacg ctggaagctg aatgctgaga aaaccctctt
    15001 ttacggttct gtaaatctgc agcagtctga gagtctcttt gcctattacg aaccgattta
    15061 tagggttatc attcagggaa atctcaatca aatcgtagaa gagttaaatc tcttggagaa
    15121 ggtagttctt gaaaatacgc cgcgtgttcc gattactaaa cagcttttta ttcagtttgt
    15181 catggatgtt ttccatttat ttgaacatct caaagctgat gacctgacag acatcgtcaa
    15241 aaccattcat gctattcaat ccttcgatga attggttcct tatatcaagg aaactctgac
    15301 cagctttttc ggtcaatacc gtatgaatga aaatgtggtc agtgtgctgg aagtcattgg
    15361 tcgtgattac caaaaagagc tttccctcaa ggatatcagt aaggctctct ttatcaatcc
    15421 tgtctatcta gggcagttga ttaagcgtga aaccgattcg acttttgcag agttactaaa
    15481 caaacaacgt attaaggctg cccaacaact tttgctttca actagtgaca gcatcgaaga
    15541 tatttgttat gctgttggtt acagtaacct tggatatttc tataaagttt ttcgaaaatt
    15601 gtgcggaaaa tcgccaaaag cctaccgaaa acaggtagaa actatactat aagatttgta
    15661 ttcctttaca aaaggtgcta taatattatc aggaggttct atcatgaaaa agaaaccaat
    15721 ttatctatgg gtcttgctaa tcttgtctgc ccttatttca gttccgtctc tgtttggaat
    15781 tgtgagtccc ttgcctagta aagaagccct tcgtgctgct cagaaacaag ttgcaggggt
    15841 caatgctcag caattagaag accagcttaa ttatacctat agagtagcag aagcatctca
    15901 ttctattttt aatgttgctt tgattgtgct atctactatt ttagtggtcg tagcgattgt
    15961 cttccttgtt cgtaaaaatt tgcaatatgc aaactatact tacgttggtt atgttttgtt
    16021 agctattatt ggttcgattt atggctatgt tggtttacaa gacgctgtgc agttggttca
    16081 agatgagagc atgcgtttga cagtgagtat tggttcaaaa gctgttagca ttttctatat
    16141 cgtcatcaat gttctcttcc tagcccttgt cttctataag atgtggcgac aacagaaagc
    16201 cttggcagaa gaagaggaaa cagaagagct tacctaagta ttgacaaaaa agaactatca
    16261 agatacaatc ttggtagttg ttttttgatt atagataaaa tcatggaggt acatatgaag
    16321 acaatttctt tagtttatat tagtttgagt ggcaacactg aaagttttgt gacgcgcttg
    16381 aaagactatc tcttgtccca gtacaaaggg attgaggttc aaaagattca tatcaaggat
    16441 ttagtcaagg aaggcaaaaa tttctatgaa atggaccatc cctatgtcgc ttttttgccg
    16501 acctatctcg aaggtgggaa tggcgtggat aacggagatg ttgagatttt gacgacaccg
    16561 gtgggagatt ttattgccta tggtaacaat gctagtaagt gttttggtgt ggttgggtca
    16621 ggaaatcgta actttaataa ccaatactgc ctaacagcca agcaatacag tcaacgcttt
    16681 ggcttccctg tattggctga ctttgaaatg cgaggcatgc tggaagatat caaacatgtc
    16741 gcagctatta tcgcagattt gtatgagttg gaaaaggaga attaatactc aatgaaaatc
    16801 aaagagcaaa ctaggaagct agtcgcaggt tactcaaagc acagctttga ggttgcagat
    16861 agagctgacg tggtttgaag agattctcga atagtataaa gttcttataa gaggtggttg
    16921 aagttgttca actgtttttt gagtataaac agtctttaag aacgtaaaaa agcataagtt
    16981 caggtcttca aaaccttgtt cttatgcttt ttatcataga aatatttgct gaattttctc
    17041 ttgaaatgaa accttatctt catctcgcgc ttgtccctta ccctgctttt ccgattgcga
    17101 gtgcgtagat aaagaagatg gcgaagccaa agaggaaaat caatcccgca atggcaaggt
    17161 gtttgagcca agaaggaaga tttttgtttt cacgcgttac caaggcgtaa ttcaaaagag
    17221 caaagaaagg tgttgtcagg aaagagccaa tcatggcaaa gcggagcatg gttgaaacct
    17281 gaccagcgaa gaacttgata atgacgatac cgatgatagc agtgatggtc atccagatgt
    17341 tcaaagattt acgattgtcc tctttttgac tgattagcag tcggagagat tcctgattaa
    17401 cgcgagaata gccatcgata acagttataa ctgttccaaa gatacagagg aaggcaataa
    17461 aggtaatcaa gtaacgggac cattcgccaa gaacagaggc atacataccc acgaattgag
    17521 agatgtattt ggctgaagca gcttcaaccg cctgccctgt aggatactga atcagtgctc
    17581 ccagtgccac aaagaagacg gctaggatag ctgttccaat ataaccagtg ttaaagtcaa
    17641 acagagcgtc ttctgtgtta aagttgacgg tctttctctt ttcagctgac caaagtgaat
    17701 tgatagctga aatttcaata ggagccggca tccatcctag gagggagacg atgaagggca
    17761 gagctgccat ttgccaaggt gtcttctcga caaaatcaga actgtattct ggatgcttga
    17821 ccgccgcaat gataactgca agaacagtcg caatggttaa ggcagacata atccatttga
    17881 ccatgccgtc taaaagtttg tagcctccaa agagtagcat agcccaaatg attgcaacga
    17941 gaatgaggga ccactgagta atgctaagtc caatcattgg gaaggcactg gcgatgatag
    18001 ctgagcacag aatggcaaca ccagccgtgt tgaccatagc cgaaaagaca ttgaggataa
    18061 agaaaatcca gagatagagt tttccttttt cggcataacc ttcaaccaaa gtctttccag
    18121 tatcagctgt gtattcagca ccaaaacgga aaaatggata tttaaagaca ttggctaaga
    18181 tgaccaagag aagtagagac caaccgtaag aaccgccagc ttgagttgag gatacaatgt
    18241 gggaacctcc aacagcggca gttgccatta ggattccagg tcccattgct tttagcttgc
    18301 ttgcccaggt tgattgattt aaggaaatag cttgcgacat gataaatact cctttttgaa
    18361 ttttcagaat aatctgtata tgtaatctat attctacaaa aaattttgga aaaatgcaag
    18421 agtattttga aaaaagatag aaaatttaca aagaagatct gaaataactg ttatgatata
    18481 gaggattgaa actagaatag tacacctctg cttctaaaac attgttagaa atcgatttga
    18541 ctgtcctgat cgatttatcc tgttcttatt tcatttcact atagtaaggt gcagcaggat
    18601 aaagaaaaac cgagaagttt ctttctcgga tttgcttatt atgaggttcc tattttctat
    18661 gctccaagag ttgattgatg tgatctactt ttttagcctc tcttttatag agaatagccc
    18721 agatgatgag atagacaatc gcaaactcga taatgagctg gaggtagaaa atccagtgga
    18781 aagggaacca accagcaaga gttgctagtg ggacaaagcc agccagcata aggaagaaat
    18841 gagtcagagt ggcacgaagc atgctccagt cacggctaaa taagcggttg ccaaagttga
    18901 agagcatacc gatggttgcc cagataagtg tgcagtagag caagaccagg gcaccgtgaa
    18961 cctgatgctg atccatcact tggcctatga gagagtaggg atttagtggt gcgtaggtat
    19021 ttggtgcata aatgagtgaa aagaggatag agaggatgag gccgataaga acaccggtag
    19081 ctgcatcgtg aaatacttgt tttttcatag ttctaatttc tccttgatgg tttttagata
    19141 acggcgtgaa gagtaggtga agctttcgtt tttcaagaaa atttctacca gaccgtttgg
    19201 cgtgagcttg aggtgagaga tggaatcgat attgatgatt tctgattggg aaatttggat
    19261 aaaattgctt ggcaagagtt taagaacctg atagagtcgc aaatcaatgc tgtaggtctg
    19321 actcgcggtt tctgctagaa ccttccgatt ctcgatatag aagcgctgaa tcttgccaat
    19381 ctcaactaga tagacctgat catcgatttt tcctttgatt ttttctcttt ggtccagatt
    19441 ttctgcgaac tcgatgactt tctggacttt ttcggtttct tgaggtgctt ggacaatcag
    19501 cttttcctcc tcgtaagtct cactaatctg tagttctact ttcataaatt tctctccttg
    19561 tttttcatac aaaattaatc tcacttcttg tacaccttta ttaatactct tcaaaaatct
    19621 cttcaaacca cgtcagcgtc gtcttgccgt aggatagttg ttgactttgt cagttttatc
    19681 tacaacctca aaacagtgtt ttgagctgtc tgcgtctagc tttctagttt gctctttgat
    19741 ttttattgag tataccacta ttttactccc tctggcaagg gactttgtct atgtggaggg
    19801 attgggctcc tatgtggtgg agtttttctg ttctttctga aatatggtat aatagcacta
    19861 attaatttct aggaaaatag atacagaaag gggctgaaag atgtctcata ttattgaatt
    19921 gccagagatg ctggcaaacc aaatcgcagc tggggaggtc attgaacgtc ctgccagtgt
    19981 ggttaaagag ttggtagaaa atgccattga cgcgggctct agtcagatta tcattgagat
    20041 tgaggaagct ggtctcaaga aggttcaaat cacggataac ggtcatggaa ttgcccacga
    20101 tgaggtggag ttggccctgc gtcgccatgc gaccagtaag ataaaaaatc aagcagatct
    20161 ctttcggatt cggacgcttg gttttcgtgg tgaagccttg ccttctattg cgtctgttag
    20221 tgtcttgact ctgttaacgg cggtggatgg tgctagtcat ggaaccaagt tagtcgcgcg
    20281 tgggggtgaa gttgaggaag tcatcccagc gactagtcct gtgggaacca aggtttgtgt
    20341 ggaggatctc tttttcaaca cgcctgcccg tctcaagtat atgaagagcc agcaagcgga
    20401 gttgtctcat atcattgata ttgtcaaccg tctgggcttg gcccatcctg agatttcttt
    20461 tagcttgatt agtgatggca aggaaatgac gcggacagca gggactggtc aattgcgcca
    20521 agcaatcgca gggatttacg gtttggtcag tgccaagaag atgattgaaa ttgagaactc
    20581 tgacctagat ttcgaaattt caggttttgt gtctttacct gagttgactc gggctaaccg
    20641 caattatatc agcctcttca tcaatggccg ttatatcaag aacttcctgc tcaatcgtgc
    20701 tattttggat ggtttcggaa gcaagcttat ggttggacgt tttccactgg ctgtcattca
    20761 catccatatc gacccttatc tagcggatgt caatgtgcat ccaactaagc aagaggtgcg
    20821 gatttccaag gaaaaagaac tgatgaccct ggtttcagaa gctattgcaa atagtctcaa
    20881 ggaacaaacc ttgattccag atgccttgga aaatcttgcc aaatcgaccg tgcgcaatcg
    20941 tgagaaggtg gagcaaacta ttctcccact caaagaaaat acgctctact atgagaaaac
    21001 tgagccgtca agacctagtc aaactgaagt agctgattat caggtagaat tgactgatga
    21061 agggcaggat ttgaccctgt ttgccaagga aaccttggac cgattgacca agccagcaaa
    21121 actgcatttt gcagagagaa agcctgctaa ctacgaccag ctagaccatc cagagttaga
    21181 tcttgctagc atcgataagg cttatgacaa actggagcga gaagaagcat ccagcttccc
    21241 agagttggag tttttcggac aaatgcacgg gacttatctc tttgcccaag ggcgagatgg
    21301 actttacatc atagatcagc acgctgctca ggaacgggtc aagtacgagg agtaccgtga
    21361 aagcattggc aatgttgacc agagtcagca gcaactccta gtgccctata tctttgaatt
    21421 tcctgcggat gatgccctgc gtctcaagga aagaatgcct ctcttagagg aagtgggcgt
    21481 ctttctagca gagtacggag aaaatcaatt tattctacgt gaacatccta tttggatggc
    21541 agaagaagag attgaatcag gcatctatga gatgtgcgac atgctccttt tgaccaagga
    21601 agtttctatc aagaaatacc gagcagagct ggctatcatg atgtcttgca agcgatctat
    21661 caaggccaat catcgtattg atgatcattc agctagacaa ctcctctatc agctttctca
    21721 atgtgacaat ccctataact gtcctcacgg acgtcctgtt ttggtgcatt ttaccaagtc
    21781 ggatatggaa aagatgttcc gacgtattca ggaaaatcac accagtctcc gtgagttggg
    21841 gaaatattaa aagtataaaa aagtctggga aaaattttca aaatcagaaa aacgcataaa
    21901 atcaggtgtt caaaaacctt gattttatgc gttttatcat ggaaatagtt acttcatgtt
    21961 ttcctaattc ttttcgaaac tctttttaaa ccacgtcagt tttatcagta atctcaaaac
    22021 agtgttttga gctaattttg ccagttttgt ctgtaacatc gaagttgtgt tttaccactc
    22081 tgcgactggt ttcctagttt gctctatgat tttcacagag tattaaattg cgattttgcc
    22141 aagtttcttt attcgtctaa aagtagagtc tgttctatgc gtctaatgta cgaattaggt
    22201 tgaccatttc aatagctcct tgtgcacact cagaaccctt atttcctgct ttggtaccag
    22261 ctcgttctat ggcttgttca attgtatctg tcgttagcac accaaacata acaggaattt
    22321 cgctatttaa actgatttgg gcgattccct tagatacctc gctacataca taatcataat
    22381 gacttgtatt ccctctaatg acagctccca agcagataat tgcatcatat tttttacttt
    22441 ttgccatttt tgatgcaatc agtggtattt caaaagctcc tggaacccag gctacctcta
    22501 tatctttctc gtttacattc tctcttttga gattatctag tgctccagat aataattttg
    22561 aagttataaa ttcattaaat ctcgctacaa caatacctat tttaatattg tttgctacta
    22621 aattaccttc ataagtgttc atttattttt cctccatatt taaaatgtga cccattcgat
    22681 ttttctttgt ttctaaataa aaactatcgt aaggattggc ttctatttcg attgatattc
    22741 tactggaaat ggtaattcca tatttttcta actgttcaac cttgtcagga ttatttgtca
    22801 gtaaatgaag tgactgaagt cccagatctt taagcatttg tgctccaata tgatattctc
    22861 ttaaatcacc ttcaaagcct aatgcaagat tggcatcaag cgtatccatg ccttgatctt
    22921 gtaaatgata ggcttttaat ttattgataa gtccaattcc tcgtccctcc tgtcgcaagt
    22981 aaagtaagac acccgaacca ttctcaacaa tcattttcat agctttatcg aattgctgtc
    23041 cacaatcgca acgtaaagag cctaaaacat ctcctgttaa acattcggag tggacccgac
    23101 ataatacatt ggcttcatcc tctatgtttc ccataataag agcaagatga tgttccccat
    23161 ttagtttatc tatatagcta attgctttga aattaccgta tctagtaggc atattgacag
    23221 ttgaaactcg ttctatcagc tgatcatata cttttctata ttcttgtaat tctttgatgg
    23281 taattagtgg aatgttgtgt ttcttcgaga actgaattaa atcatctgtt ctcatcattt
    23341 tgccatcatg attcattatt tcacaacata ggccacactc ttttagtcca gctaatttta
    23401 ataaatcaac agttgcttct gtgtgtccat ttctttctag aacaccacct ttttttgcaa
    23461 ttaaaggaaa catgtgtcct ggcctgcgaa aatcagaggg tgttatatct tcagctacac
    23521 acatacgtgc ggtcagtcct ctttcctcgg cagaaatacc tgtggtcgtt tctttataat
    23581 caattgaaac tgtaaaagca gtcttatgat tatctgtatt gttttcaacc ataggtgaaa
    23641 gcattaattg attagctaaa ctttcgctca taggcataca aattaatcct ttggcataag
    23701 tagccataaa attaacattt tctgttgtag ctgcttgtgc agaacaaatt aagtctcctt
    23761 cattttctct atccttgtcg tctataacaa gaacaagtcg tcccttctgc aatgcttcta
    23821 atgtttcttg tatttttcga tattccattg actgattatc ctttctgcta aaatccattt
    23881 tgatataata gttccttaga tatttctgat tttggagagt tatccatcag tttttgcaca
    23941 tatttaccta agatatcatt ttcaagattt actgtactcc cgacttgttt acttttaaga
    24001 atggtttgtt tcaaggtatg agggataaca gatactgaaa agtttacttt ggagacttta
    24061 gcgacagtca gactaatgcc gtcaattgta atagatcctt tttcaactat taaatctaaa
    24121 atttcttttt gtgtgttgat ttgataccat acagcattat catctttttt tattgacgag
    24181 atttttcctg taccatcaat gtgtcctgta acgacgtgac ccccaagtcg accgttgata
    24241 gataaggctc tttctagatt cacctcactt ccatgtttta atagagtaag agctgttcga
    24301 ctccatgttt cattcattac atcaactgta aaggattgat gattgaaatg agtaactgta
    24361 agacagatac catttactgc tatactatcg cctaaatgga tatccgttaa tatttttgag
    24421 gctttaattg atagtttaca attacgagag tctttctgta ttctttcaac ttttccgatt
    24481 tcttcaatta ttcctgtgaa catggataaa tcacttcact ttctatgaga tagtcatttc
    24541 ctatttgaga aaaagcataa ggtttcaatc taatagcgtc atttggcaaa gaaatgcctt
    24601 cacctccgac aggaaacttg gcactgcctc caaaaatttt tggtgcaata tatattttca
    24661 gctcatcaac aatttgttgt tccaaagcac tccaattcat tagactgccc ccttctagaa
    24721 ctaggctatc aatctgcatg tttcctagat gttgcattaa actcgataag tctatatgat
    24781 tgcctttttt ctttatggaa agtatttcac agccatgatt ttgatatagc ttcattttat
    24841 ttttgtcttc agaggaagtg gcaatgtaag ttttaatatc atttgctgtt tttacgattt
    24901 tagaggtaag aggagttcgt aaatgtgtat cgcatatgat acggatagga tttttccctt
    24961 cctccaatct acatgtcagc aaaggatcgt cttgaataac agtattgact cccaccataa
    25021 ttgcactaac atggtgtcgt aactgatgca catgctttct tgcttcttct tcagtaatcc
    25081 atttggattg atttgtttta gtggctattt ttccatccat tgacattgca tatttcataa
    25141 aaacataggg tacatgctgg gtaatatact ttctaaaact ttttattaag ttaagacact
    25201 cattttctaa aattccaaca gtaacttgaa gattattttc ctcaagtatc tttactcctt
    25261 ttccagatac aataggatta cagtctaggc ttccaatgac tactcttgta ataccactat
    25321 cgattatagc atctatacag ggaggtgttt tcccgaagtg acaacagggt tcaagtgtta
    25381 cataaagcgt cgctccgaca ggggattctc tacagttttt aagagcattt ctctcagcat
    25441 gtgggccacc aaaaaactca tgataacctt gtccgatgat gtgattatct tttacaataa
    25501 ctgcgccgac cataggattg ggattgacgt aaccagcccc tttttgtgcc agttttattg
    25561 ctaatttcat atattttgaa tcgctcatct cactacctcc aaaaaaaata taccttgaat
    25621 aggggactac tcaaggcata caaaagaaaa cttatgcgat taacaaaaat actctgaaat
    25681 gacaagtaat catttcagag cacgcaaaaa gcacaaatat acttttatct tctttcatcc
    25741 agactatact gtcggctttg gaatttcacc aaatcatgcc tttcggctcg tgggctatac
    25801 caccggtagg gaattacacc ctgccctgaa gatagttatt caattacaga tgattatagt
    25861 acttaatttt gaatatgtca acagataaat accgattgtt tttgatatac tgtatttgtg
    25921 ataatcgatt ctcgctcctc ggataaagaa aatatgatat actagataaa cgaaataaga
    25981 gagaaggaat actatgtacg catatttaaa aggaatcatt accaaaatta ctgccaaata
    26041 cattgttctt gaaaccaatg gtattggtta tatcctgcat gtggccaatc cttatgccta
    26101 ttcaggtcag gttaatcagg aggatcagat ttatgtgcat caggttgtgc gtgaggacgc
    26161 ccatttgctt tatggatttc gctcagagga tgagaaaaag ctctttctta gtctgatttc
    26221 ggtctctggg attggtcctg tatcagctct tgctattatc gctgctgatg acaatgctgg
    26281 cttggttcaa gccattgaaa ccaagaacat cacctacttg accaagttcc ctaaaattgg
    26341 caagaaaaca gcccagcaga tggtgctgga cttggaaggc aaggtagtag ttgcaggaga
    26401 tgaccttcct gccaaggtcg cagtgcaagc aagtgctgaa aaccaagaat tggaagaagc
    26461 tatggaagcc atgttggctc tgggctacaa ggcaacagag ctcaagaaaa tcaagaaatt
    26521 ctttgaagga acgacagata cagctgagaa ctatatcaag tcggccctta aaatgttggt
    26581 caaataggag cagagaatga caaaacgttg ttcgtgggtc aagatgacaa acccgctcta
    26641 catcgcctat catgatgagg agtggggtca gcccctccat gatgaccaag cattgtttga
    26701 gttgttgtgt atggaaacct atcaggcagg cctgtcttgg gaaacggtac tcaacaaacg
    26761 ccaagctttc cgagaagcct ttcatagcta tcaaattcac tcagtcgcag agatgactga
    26821 gactgaattg gaagccatgc tggagaatcc agctatcatt cgaaatagag ccaagctttt
    26881 tgctacacgc gctaacgccc aagtccttct acagttacag gcagagtacg gctcttttga
    26941 tgcctatctt tggtcttttg ttgaggggaa aattgtcgtt aacgatgttc ctgattatcg
    27001 ccaagcgcca gctaaaacac ccttatctga gaaattagcc aaagatctca aaaaacgagg
    27061 cttcaagttc acaggcccag tcgccgtatt gtcttttcta caggctgcag ggctagttga
    27121 tgaccacgag aatgattgtg agtggaaagg tcttaaatga tgtctaacaa aaataaggga
    27181 attctgattt ttgcgattct ctatacagtc ctctttgtgt ttgatggcgt taaattgctg
    27241 gcttctttaa tgccatctgt cattgcaaat tatcttgttt atgtagtttt agctttatat
    27301 ggctccttct tgttcaagga tagattgatc caacaatgga aggagattag aaagactaaa
    27361 agaaaattct tctttggcgt cttaacagga tggctctttc tcattctgat gactgttgtc
    27421 tttgaatttg tatcagagat gttgaagcag tttgtgggac tagatggaca aggtctaaat
    27481 cagtctaata ttcaaagtac ctttcaagaa caaccactac tgatagctgt ttttgcttgt
    27541 gtcattggac ctctggtaga agaattattt ttccgtcagg tcttattgca ttacttgcag
    27601 gaacggttgc caggtttact aagcattatt ctggtaggac ttgtttttgc tctgactcat
    27661 atgcacagtt tggctctatc agagtggatt ggtgcagttg gttacttagg tggaggcctt
    27721 gccttttcta ttatttatgt gaaagaaaaa gagaatatct actatcccct acttgttcac
    27781 atgttaagca acagcctctc cttaatcatt ttagctatca gtatagtaaa atgaaataag
    27841 aacaggacaa atcgatcagg acagtcaaat cgatttctaa caatgtttta gaagtagagg
    27901 tgtactattc tagtttcaat ttgctgtaat atgtgatgaa aataccagta atgataccga
    27961 gaaaaaagct gagaaacttt tcccagcttt atttgttata gtcaaagcga atgacttgtt
    28021 cctgtgcatc tacatgagca tggaccccaa agggtacaat tgctcttgga gttgcgtggc
    28081 cgacattcag attatagaca atcgggatat tgctgtcaat gatatccaat agtgcctctt
    28141 tatagtcgtc atggaaagtt tcatccatag gttttccgac caagagtcca ttgatgaccg
    28201 cgaatatgcc agtgtccttt aaagttagca acatcttttt gaagtcttct ggcttaggct
    28261 tttcttcgct tgtttcgagc aagaggattt tcccttccca gtctgacaag tcagggaaaa
    28321 gtttgtattt ttggcagagt tccgtgctat ctgcgtatcg agagttgtca aagatatcgt
    28381 agagggattc gaggcaacca ccgaggattt ttccctcgaa ctgagcactt ccttgcaaca
    28441 agtcaaaacc tgtatttgta tgactgacac gaggtgttcc cagggccgtg ggactaaaat
    28501 cagttcgttc ctcataccaa acgtcactag ggcggatttc tgaaattctt cccgtcttaa
    28561 tcaattcttt aaagtagtga aggctatagg ctagcatttc tttgtctaat tcacaaatgt
    28621 ctgctaaaaa ggattgacca taaaaagtct tgattcctaa tttatgcaac atgaggtggt
    28681 tcatggttgt atccgagaag ccaagaaaaa ttttttgctt gataaccttt tggagttggt
    28741 cattttcaaa aagataaggt agcaagcgat aggtatcgtc tccaccgatg gcacatagga
    28801 tcatgtcgat gctatcatca gaaaaggcat gaatcaaatc ctctgcacga gcttcaggat
    28861 ggtccttgat aaagtctaat ccttttaacg aatgggacaa aaagatggga ttgagtccca
    28921 gatccttgag acgttggata cccaagtcca cttcgtgttt gacaaaatcc tctccgataa
    28981 tgccactaga taaactaaca ataccaatag tagaaaccat atctcatcct cctagaaata
    29041 gattgagcct attttatcac aaaatataag agaaagctat gtgggatttc agaagaatac
    29101 ttttaatact cttcgaaaat ctcttcaaac cacgtcagtt ttatctgcaa cctcaaagct
    29161 gtgctttgag caacctgcag ctagcttcct agtttgctct ttgattttca ttgaatataa
    29221 ctcaaaaaag taagtgaaaa agcaaccgca cctgcagttg cttttatcgt tttcttattc
    29281 ttgatagcgt gttggtcctg cacttgcact acggatattc aagcgtttct tgagatagaa
    29341 gcgaagggct aggagggctg ctccaataat agctagtggc aatggatcca gtactgggtt
    29401 aaggctagct ggtaggaagc ttgttgcaaa gaagacaagc aaccaaagga acatagaagc
    29461 taggataact agtacagatt tccagaaagg tggacgttga ctgcgatcca tatctgatcc
    29521 atagtattgg taaacaaagt agtacatcaa gtagaaggca aatccaccaa ccagtccaac
    29581 taatagaaga gtaatcaatc catagccgaa agcttggtct gccgcaaaga aggttgtgag
    29641 ggcgctgaca agggcaaaga ggctagtgat gaaaagagct gagtccataa tcatcagttt
    29701 tgggtcatca ttttcttttg gatgctcttt ttcgtactgc tctttgacag tgaagctatg
    29761 agcccaatga gttggtgcgc cgtataggga acgggcagtt gtacctttag attgctcctc
    29821 aaggatttgg ggaatgactt cctcaaaaat agtctggatt tcagcgtctg ttttcccatc
    29881 tttgatgaat tgttgggtag caatgtggac aaactcttgg tttttcttag ttaatttttg
    29941 tagatcaatt tgagacatag gaactcctct tagaaccatt ttttatggat gagatagaga
    30001 gtgagcgaga cactcatagc aaaggcgata aagacgatta accagaaggc atttggctct
    30061 ccgtttaggg ggatttcatt atccttaaag ttcatcccgt aggcagaaaa gaccatggtt
    30121 gggatggaca tgacgatggt cacaagggcc aaggttttca tgatgttgtt ctggttgtta
    30181 gaaatgatag aggcaaaggt ctctgtcata gaatgcaaga cgtttccata aatatctgcc
    30241 atctcgatgg cctgttgggt ttcaatcagg gtgtcttcaa gcaggtcttc gtcctcaagg
    30301 tatttcttga tattgctggt tgaactggtc aatttcttaa tcacgcgctc atttgttttg
    30361 agggaggcct tgaaatagac gatagttttt tccaattcca tgagctcaat caattcttca
    30421 ttacgagttg attgatgcag ttgactttcg atttgttcac tcttgcggtc gattgaacga
    30481 agggctgtta ggtaaagctc tgcattgcga taaagaattt gaaagataaa acgtgaacgc
    30541 atgaaggtat agaaattacg caatcgacgg ttgataaaga catcaaggac aggtagtggt
    30601 tccaaacacg tagtgataat ggtttcctca gtgatgataa taccaagcgg gatggttacg
    30661 tagtaggtgc ggttatttct ttcctccgtg accggcacgt ctacgataat cagggtatac
    30721 tcgtcttcaa tggtaatacg agacatttct tccgcatcga gcggtgctcg aaggtcggca
    30781 atatcaatat cgaaggtgtt ggcgatttcg agtgattcat tttgagtcgg attgacgaga
    30841 ttgatccaag tacccggttc aagcgtatcg atctctttaa attcagttgt tgtagagaga
    30901 aaaacttgtt tcatatccct tatcctttct catttttcag attttttcac actgtactat
    30961 tatactacaa aatcggcctt ttgggtaata gaatctcact ttttttggta taatggtaag
    31021 caataatgga ctagaaagaa caaagatgca agataaaatt gtcattcatg gggcgcgtgc
    31081 ccataattta aaaaatattg atgtggagat tccgcgagac aagttggttg tcgtgactgg
    31141 cttgtcaggt tcagggaagt ccagtctggc ttttgatact ctctatgcgg agggacaacg
    31201 tcgctatgta gagagtttgt ctgcctacgc tcgtcagttc ttgggaaata tggaaaaacc
    31261 agatgtagat gccattgatg gtctcagccc agctatttcc atcgaccaga aaacgactag
    31321 taaaaatcct cgctcgacgg tgggaacgac gactgaaatc aatgactatc tgcgtctcct
    31381 ctacgcacgt gtggggacgc cttactgtat caacggacat ggagctatca atgcttcttc
    31441 tgtggagcaa atcgttgata aggttttgga gttacctgaa cgccagcgct tgcagatctt
    31501 ggctcctgtc atccgcaaga aaaaaggcca acataagagt gttatcgaga aggttcagaa
    31561 agacgggtat gttcgtgttc gtgtggatgg ggaagtctat gatgtgaccg aagtgccaga
    31621 gttgtctaag agcaagcaac acaatatcga tgtcgtggtt gaccgtattg ttatcaagga
    31681 gggcattcgt agccgtctct ttgattccat tgaagctgcc cttcgtatcg cagaaggtta
    31741 tgtgattatc gacaccatgg acgactctga gttgttgttc tctgagcatt atgcctgtcc
    31801 agtttgtgga tttactgtcc cagagttaga acctcgtctc ttctctttca atgctccctt
    31861 tggctcttgt agtgagtgtg acggcttggg gattaagctg gaggtggata ctgatttggt
    31921 agtgccagat gccagcaaaa ccttacatga gggagcgctg gcaccttgga atcctatctc
    31981 atccaactac tatccaaaca tgttagagca ggccatgaaa gtctttggag tggctatgga
    32041 taagcctttt gaggacctgt cagaagaaga taagaacttg attctctacg gctcagatgg
    32101 taaggaattc catttccatt atgaaaatga atttggtggt gtgcgcgata tcgacattcc
    32161 gtttgaggga gttatcaata atatcaaacg tcgttaccat gaaaccaata gcgattacac
    32221 tcgcactcag atgcgtctct acatgaatga gctgacctgc ggaacctgtc aaggctatcg
    32281 tctcaatgac caggccttgt ctgtccgtgt gggcggccag caagggccac atattggaga
    32341 aatctcagac ctgtctatcg ctgaccactt ggacttggtg agccagttaa ctttgtctga
    32401 aaatgaagcc atcattgctc gtcccattct caaggaaatc aaggatcgtt tgaccttcct
    32461 taataatgtg ggtcttaact atctaaccct gtcacgttca gcaggaaccc tttcaggtgg
    32521 ggaaagtcag cgcattcgtt tggcaaccca gattggttcc aacctatcag gtgtcctcta
    32581 tatcctagac gagccgtcaa tcggtcttca ccagagggac aatgaccgcc tgattgccag
    32641 tctgaaaaag atgcgtgact tgggcaatac tcttatcgtg gtggaacacg acgaagatac
    32701 catgcgtgaa gctgattatc tgattgacgt tggtcctggt gccggtgttt ttggtggtga
    32761 gattgttgca gcaggtacac ctaagcaagt ggctcgcaac agcaagtcta tcacaggtca
    32821 gtacttgtca ggcaaacgtg tcatcccagt accagaagag cgccgtgtcg gaaatggtcg
    32881 ttttattgaa gtgacaggag cgcgtgagaa caacttgcaa aatgtcacgg ctcgctttcc
    32941 actaggaaaa tttatcgcag tgacaggtgt gtcgggttca gggaaatcga ctctaatcaa
    33001 cagcattctc aaaaaagcca ttgcccagaa gctcaaccgt aattcagaca aacctggtaa
    33061 gtttaagact attacaggga ttgagcatgt cgaccgtttg attgatattg accagagccc
    33121 tatcggacgg acgccgaggt ctaacccagc tacctataca ggagtttttg acgatatacg
    33181 tgaccttttt gctcagacaa atgaagccaa gattcgaggc tacaagaagg ggcgcttcag
    33241 tttcaacgtc aagggaggtc gctgtgaagc ttgctcaggt gacgggatta tcaagattga
    33301 gatgcacttc ttgccagatg tttatgtggc ttgtgaagtt tgccacggaa cccgctataa
    33361 cagtgaaacc ctagaagttc actacaagga aaagaatatt tcgcaggtct tggatatgac
    33421 ggtcaacgat gcggtagaat ttttccaaca cattccgaaa attcaacgca aactccagac
    33481 catcaaggat gtaggtctag gctatgtgac cttgggacag ccagctacca ccctttctgg
    33541 gggagaagcc caacgtatga agttagctag cgaactccac aaacgctcga caggaaaatc
    33601 tttctacatt ctggatgagc cgacgacagg gcttcataca gaggacattg ctcgcctgct
    33661 caaggtttta gctcgctttg tagacgccgg caatacagtc ctcgtcatcg agcacaatct
    33721 ggatgttatc aagacggcag accatatcat tgacttggga cctgagggcg gtgtcggtgg
    33781 tggaaccatc atcgtaacag gaactccaga agaagtagct gccaacgaag ccagctatac
    33841 aggacactat ttgaaaggaa agttacatca tgaataaacg cgtacaagca tttctagcta
    33901 aaatgcaaga aaaagaacta gatggtatca tcatcaacaa tcttaaaaac gtctattatt
    33961 tgactggttt ttggggctca aacggaacag tctttatcag ccgtgaccgt caggtcttag
    34021 tgacagactc tcgctatatc atcgcagcta agcaagaaac cagtggtttt gagattgtgg
    34081 ctgatcgtga tgaattggct gtcattgcag gaattgttaa ggacatgaac ttgactcgta
    34141 tcggttttga agatgagatt tcagtgtctt attaccaccg tatgcaggca gcttttgcag
    34201 gtttgaactt gcttccacaa actcagtttg tggaaggtct tcgtatgatt aaggatgagg
    34261 cagagattgc agcgattcgc aaggcttgtt ctatctcaga ccaagctttc cgcgatgcgc
    34321 ttgactttat caaaccagga aaaactgaaa ttgagattgc caacttcctt gatttccgca
    34381 tgcgtgagtt gggagcatct ggcttatctt ttgatacgat cctagctagc ggtatcaatt
    34441 cttctaaacc ccatgcccat ccaatgcaca aaccagtgga gttgggagaa gccattacca
    34501 tggacttcgg ctgtctctat gaccactatg tcagtgatat gacccggact atctatctag
    34561 ggcatgttag cgatgagcag gcagagattt acaatacggt tctaaaagct aaccaagcct
    34621 tgattgacca agctaaggca ggcttaggtt tccgtgactt tgacaaaatc cctcgtgata
    34681 ttatcattga ggcaggttat ggtgactact ttactcacgg cattggccac ggtattggtc
    34741 tggatatcca tgaggaaccc tactttagtc agacttctac agaaactatt aagacaggta
    34801 tggccttgac cgatgaacca ggtatctata tcgaaggcaa atatggcgtt cgtatcgagg
    34861 atgatatcct gattacagag acaggttgtg aattattgac cctagctcca aaagagttga
    34921 tagtcattta gttgtttgtc aagaaaaaag tacgaaaatg acgaaaaaag tcaaaaaaaa
    34981 ttaaaaatag gtcgcaagtc ggatgttttt tatggtataa tagactaaac tgatagtaac
    35041 atgtagcgaa aggggtaggt acatgattaa aatttataca gtctcaagtt gtactagttg
    35101 taaaaaagca aaaacctggc tcaatgccca ccagttaagt tataaagaac aaaaccttgg
    35161 taaagaagga attacgagag aagaattact ggatattcta accaaaacag ataacggaat
    35221 agccagcatc gtttcgtcta aaaatcgcta tgccaaagcc cttggagtgg atattgaaga
    35281 tttgagtgtc aatgaagttc tcaatctgat tatggaaaca ccgagaattt taaagagccc
    35341 aatccttgta gatgaaaaac gcctgcaagt tggctacaag gaagacgata ttcgtgcctt
    35401 cctaccacgc tctgtccgta atgtagaaaa tgcagaagca cgtttgcgtg cagctctata
    35461 aacatcaagg ctgggagcac ttcccagtct tattctattt taatttcaaa aagaaagaag
    35521 aaagaaatga aaaaaatagt tcttgttagt ctagctttcc tttttgtcct ggttggttgc
    35581 ggacagaaaa aagaaactgg accagctaca aaaacagaaa aagatacgct tcagtcggca
    35641 ttgccagtta ttgaaaatgc tgagaagaat acagttgtaa ctaagacttt ggtcttgccc
    35701 aagtcagatg atggtagcca gcagacacaa acaattactt acaaagacaa gacttttttg
    35761 agtctagcta tccaacaaaa acgtccagtc tctgatgagt tgaagactta tattgaccaa
    35821 catggagtgg aggaaactca aaaagctctt cttgaagcgg aggagaagga taagtctatc
    35881 attgaagctc gtaaattggc aggtttcaaa cttgaaacaa aactattgag cgcaacggaa
    35941 cttcaaacaa cgactagttt tgattttcaa gttctggatg tcaagaaggc ttcccagttg
    36001 gaacatctga agaatattgg tttggaaaat cttttgaaaa atgaaccaag caaatatatt
    36061 tcagatagat tggcaaatgg cgcgacagaa caatagaaaa tccaaataaa tagactgcct
    36121 gaattgtaga aggtaaggaa ttatgctata atggtactat tctaaggaaa gagggtgtta
    36181 gaatgggatt tactgaagaa acagtacgtt ttaaattgga cgattccaat aaaaaagaaa
    36241 ttagcgaaac tttgacagat gtttatgctt cgttgaacga taagggttac aacccaatta
    36301 accaaatcgt aggttacgta ttgagtggag accctgccta cgttcctcgt tataataatg
    36361 cacgaaatca aatccgtaag tatgagcgtg atgaaatcgt tgaggaattg gttcgctact
    36421 acctcaaagg acaaggagtc gatctataac ctatgagaat tatgggattg gacgtcggtt
    36481 caaaaacggt aggggtggcg attagcgatc cgcttggttt tacagctcaa gggcttgaaa
    36541 tcatccagat aaatgaagaa caaggccaat ttggttttga ccgcgttaag gaattggttg
    36601 atacttacaa ggtggaacga tttgtagtgg gcttgcctaa aaacatgaac aatacaagtg
    36661 gaccgcgcgt agaagctagt caagcctacg gagcaaagct agaagagttt tttggtttac
    36721 cagtagacta tcaggatgaa cgcttgacaa cagtggctgc tgagcgcatg ttgattgaac
    36781 aagcagatat cagtcgcaat aagcgcaaga aagtcattga taagttagca gctcagctga
    36841 ttttacaaaa ttatttagat agaaaatttt aatataaagg agaggctatg tcacacgatc
    36901 ataaccacga ccacgaagaa cgtgaactaa tcacactagt agatgagcaa ggaaatgaaa
    36961 ccttgtttga aatccttttg acaattgatg gaaaagaaga atttggtaaa aactatgttc
    37021 ttctagtacc agttaacgca gaagaagacg aagacggaca agttgaaatc caagcttact
    37081 cattcattga aaatgaagat ggaacagaag gcgaattgca accaatccca gaagactcag
    37141 aagacgaatg gaacatgatt gaagaagtct tcaacagctt tatggaggag tgatacagtc
    37201 tgggagactg tatcagccca actccagaaa tggggaagag ttggaacgtc cagtgaacat
    37261 ttttacccca actcttagaa attgagaaga gttggaacat ctagtggatg tttttagccc
    37321 atattcacat tcatagtagc tagttattag ctaaccaggt aagaggtcgg gacgaaaatc
    37381 ctggccttgt ttttattagt aaccatactt taatgcgata attgattgga cttttgtcaa
    37441 ggagatgtgt atgtttgaag tagaagaatg gctccatagt cggattggtt tgaattttcg
    37501 atcaggtttg ggccgaatgc agcaagcggt ggatttgtta ggaaatcctg agcagtctta
    37561 ccctattatc cacgtaacag ggactaatgg gaaaggatct accattgctt ttatgaggga
    37621 attatttatg gggcatggca aaaaagttgc gacctttacc tcccctcata tcgtctctat
    37681 caatgaccga atctgcatta atgggcaacc tatagcagac gcagacttta tccgtttgac
    37741 tgatcaggtc aaggagatgg agaaaacgct tctgcaaact cctgcccagt tgtccttttt
    37801 tgaattgctg accttagttg cttttcttta ttttagggag caagaggtgg atttggtttt
    37861 attagaagtg ggaattggtg gcttacttga cacgaccaat gtggtaactg gagagcttgc
    37921 tgtcatcacc tccattgggc ttgaccatca agaaaccttg ggtgatagtc tagaagcaat
    37981 tgcagagcag aaagctggta ttttcaaggc tggtaaaaag gcagtgattg cgaaattgcc
    38041 tccagaagct agacttgtct gtcagaaaaa agccgaatct ttagctgttg acctttatca
    38101 ggcaggtcaa gatttttcag tgctgaatgg tgatttttca agctctttac taaatctttc
    38161 acagctgaac ataggcttag aaggagccta tcagcaggag aatgcggcct tggcgttgca
    38221 aacttttctt ctttttatgg gagaaagaaa ggaagctatt gatgaacagg ctgtaagaaa
    38281 ggccttggaa cagacccatt gggctggtcg cttggagcgt attcgcccac agatttactt
    38341 ggatggtgct cataacctcc ctgccttgac tcgcttggtt gagtttatca aagaaaaaga
    38401 gcaggaaggc tatcgacctc aaatcctctt tggatccttg aaacgtaagg attatcaagg
    38461 gatgttgggt tatctgactg aaaatttacc tcaagtggaa ctcaaaatga ccggctttga
    38521 ctatcaaggg tctttggacg agacagatgt aacaggttac catgtaattc cctcttaccg
    38581 agaatttatc agcagtttcg aagaaagggc agacgctcaa gacttgctgt tcgttacagg
    38641 gtctctctat tttatctcag aagtacgggg ctacctgctg gaccgtgagc agataaattg
    38701 accccctttt ttgaacttgt tatactaggg gaattgctgg ttagagaaaa tttctctaaa
    38761 ttggtagcag aaaggaaatt catcatgaaa ttaaaaagat tcacactttc tcttgcttct
    38821 ctagcaagtt ttagtctctt agtagcttgt tcacaaagag ctcaacaggt tcaacagcct
    38881 gttgctcagc aggtccaaca gcctgctcaa cagaatacca atactgcaaa tgcaggaggt
    38941 aaccaaaatc aagcggctcc agtacaaaac caacctgttg ctcaaccgac cgatattgat
    39001 gggacttata ctggtcagga tgacggagac cgtatcactt tagtggtaac tggaacgact
    39061 ggtacatgga ctgagctcga atctgacggg gatcagaaag tcaaacaggt tacatttgat
    39121 tcagcaaatc aacgcatgat tattggcgat gatgtcaaaa tttacactgt aaacggtaat
    39181 caaatcgtcg tagatgatat ggatagagac ccatcggacc aaatcgtttt aactaaataa
    39241 gactatgcaa gtagaaaggg ctggattttt cagccttttt acttttacct agaaaataat
    39301 cataaatact tgccttctac cgaatacctg cgttatacta gtatcaatta cttgttttta
    39361 gcattcaaac tatcaaggag gggatatgaa atataggaaa tttcaattat tgatgtccaa
    39421 gtatggcttt agtctttcga ttatgctact tgaactttgt cttgtttttg gtctctttct
    39481 ttatttagga cgcatggctc ccattttatg gattactgtc ctcattctac tgagtatcat
    39541 cacaatcatt tcgatagtca accgtaatac gactcctgag aataaggtaa cctggttgtt
    39601 agtagccttt gtgccagtat ttggtccctt gctctatctg atgtttggtg aaaggcgatt
    39661 gtccaaaaaa gaaatcaaac aactgaagaa gctaggctct atgcatttcc aagaagcaaa
    39721 tagccagcta ctaaaagaga aattaaaaga aagtgacaag gcagcttatg gagtcatcaa
    39781 gtccttattg agtatggata ccaatgctga catctatgat caaactgcct ctacattttt
    39841 tcctaacgga gaagctatgt ggaaaaagat ggtagaagat cttaaaaagg ctgagaaatt
    39901 tattttcttg gaatattaca ttatagaaga aggtttgatg tggaatcgca tactagatat
    39961 actagagcaa aaggtagctc agggtgtaga ggttaagatg ctctatgatg atatcggctg
    40021 tatggctact ttaacaggag attatgcaca tcgacttcgt cagctgggca tcgaggccca
    40081 taaattcaat aaagttattc ctcgtttgac agtggcttat aataacagag atcatagaaa
    40141 aatattgatt gttgatggtc agatagccta tactggtggg gtcaatctgg cagatgagta
    40201 cattaaccac gtcgagagat ttggttattg gaaggatagt ggaattcgct tagacggact
    40261 agcagtaaaa gccctgacac gcttattttt gaccacttgg tacattaatc gaggagaaat
    40321 tagtgatttt gatcaatatc atttagaaaa tcattctatc ccgagtgacg gtttaaccat
    40381 tccatacgga agtggaccca agccaatttt tcgagcgcag gtagggaaaa aagtttatca
    40441 gagtttaatc aatcaagcaa cagaatcggt ctatattacg acaccttatt tgattataga
    40501 ttatgattta acagagacaa tcaaaaatgc agctatgaga ggggtcgatg ttcgaattat
    40561 caccccttac ataccagata agaagttcat tcagttagtc acgagaggag cttatcccga
    40621 ccttctttct gctggtgttc ggatttatga gtatagtcca ggttttattc atagtaagca
    40681 gatgttggta gacgaagatt ttgcggtggt ggggacaatc aatctcgact accggagctt
    40741 ggtacaccat tatgaaaatg cagtcttact ctataaaact ccttctataa gggaaatcgc
    40801 ccgagatttt cgaaatatat ttgcagattc tcaggaagtc tatcctcatt ctatcaaaac
    40861 gagctggtat caaaagcttg taaaagaaat cgcccagcta ttcgccccta tcttataaga
    40921 aatctagaac ttaggataca aaccagacct atttttcttg tcttttacta atagaaagaa
    40981 agaggagggg aattcatgct aactaaggaa gaagtgaatg atatgattga gttcaaacag
    41041 acacatttac accgctttcg ggaaattgaa acctttgtga aactatgcaa aaaatcactc
    41101 aaacagccat cgcaagctga caatcctagg actttttgat atactaagac agataaattg
    41161 aacaggagaa acgatatgag tgtgtatggt agagtagaag aagttcataa ggagaatcgt
    41221 gaacctctag aataccaaat cgaacaagaa tcgcatcatc gtgaatcaag tcgtcttcct
    41281 ttggtgaaaa ttttactatg gagtacgctt gtaacgggga taactctagg agtaccgcta
    41341 ttactcgact taatgagtgc acaagaagtg caggattttt atgcaggttg ggggcttcat
    41401 cagacaggga agatttacag cgactattat ggaagtcaag gtttgcttta ttatttgctg
    41461 acttacgtga gtcagggcgg atttttcttt gccatctttg agtggttagc cttggtagca
    41521 ggaggatttt tcctttttag atcagcggac accttgacag agcaaggaga ccaagctgga
    41581 catctggtga ctatttttta catgctagtt acaggtcttg cttttggtgg aggctatgcg
    41641 actcttttag cgcttccttt cttattcgca gcctttagtt tagttgcggc ttacctaagc
    41701 aatccaagcc atgataaggg atttgtacgg attgggctag ctttggcagg cggatttttc
    41761 tttgctccct tatcatcgct cctgtttatt gctgtagtga gtttaggctt gttggtcttt
    41821 aaccttgggc atagacgctt tgcgcatggg ttttatcagt ttcttgcagt ggctttaggt
    41881 ttttcacttg tcttttatcc aactgcctac tatagtgctg caacaggaag ttttggggat
    41941 gcgattagtg gtattcgtta tcctattgac agtattcgct ttgattttac ttctaaaatt
    42001 ttagagaata tgttttttta tggcttgttg tcccttggtt tgggatttgt ggtttgtatc
    42061 tttttaggtc tctttcaatc caaacccttt aaactatacg tcatttcggt tcctgcaagt
    42121 cttgtggtaa ttttaggttt gattttactt ttcttttcac aagagcctct gcacgcttct
    42181 tatttgatgg tcgtcttccc tgttttccta cttttattgg taaccaatat taagagtcaa
    42241 cagagggggc gtagtgctag aagaagccga agagaaacgc cagttagcct atggagtcgt
    42301 ttcttcaaag gaaatctata tctgctagtt tttgggtttg tctatctttt gtctgttcct
    42361 tttttgatga agtttgtcct ttatccagta ccttatcaag aacgtaatcg tcttgctgat
    42421 ttggtaaaag aggagacaaa tacggaagat gctatctatg catgggatga tactgcgact
    42481 ctttatcgta agagtgagcg cttgtcgcca tcggcgattt tgtccccgtt gcactataca
    42541 gcaactgagg aaaatcgtaa taagttactt aatgacttga aagaaaaaca acctaaggtg
    42601 attgtggtaa atgacaaggt ggtagtctgg tctgaagtgg aaacactttt aaaagaaaat
    42661 taccaacaag taaagactga ttactcagag tttaaagtct ataaaattaa ataaccaaat
    42721 caatatcttg tgtattttta aaaattttag gatttttaac acaagatatt gatttttctt
    42781 tttagagtgg tataatactt tttagaaaga acattttaga aaagagcatg cgtatgattg
    42841 cactagaaga aaaaattaca attttgccaa ctctcttcgt cgagaaacga gatgggagac
    42901 gtgttgtatt tgatgtggac aagattgaca aggctctcca caaggcggct gacaaggtta
    42961 tggatgtgac acccctggtt gaaaaacgcc tcaatgatct gactgagcga attattacag
    43021 aaattcatag tcgctttcca cagggaatta agatttacga aattcaaaat atcgtagaac
    43081 atgaactcct tgaagccaaa gaatatgcgc tggctgagga gtatattact tatcggacac
    43141 agagggattt tgagcgctca aaagcgacgg atatcaactt tagtattcat aaacttctca
    43201 acaaagacca gacagttgtc aatgaaaatg ctaataaaga cagtgatgtc tttaacactc
    43261 agcgtgattt gacagcaggg attgttggga aatcaatcgg actgcaaatg cttcctaagc
    43321 acgtagccaa tgcccaccaa aagggggata tccactatca cgatttggac tacagtccct
    43381 atacccctat gaccaactgc tgtttgattg attttaaggg tatgttggaa aatggtttta
    43441 agattggaaa tgcagaggta gagagtccca agtctatcca gactgcgaca gcacagattt
    43501 ctcaaatcat tgccaacgtt gcttctagcc agtacggtgg ctgttcagct gaccgtatcg
    43561 atgaaatttt ggcgccttat gcagagaaga attatcaaaa acatctcaaa gatgcagaag
    43621 agtgggtatt gcctgaaaaa cgggaagatt acgcttggaa gaaaacgcaa aaggacatct
    43681 acgatgccat gcaatctctt gagtatgaaa tcaatactct cttcacttca aatggacaaa
    43741 caccttttac ttcgttaggt tttggtctgg gaaccagtcg ttttgaacga gaaattcaaa
    43801 aagctatttt aaacattcgc atcaagggtc ttggttcaga acaccgtacg gctatctttc
    43861 ctaaacttat tttcactctg aaaagaggcc ttaatttaga ggaaggaact cccaactacg
    43921 acatcaagca gttggctctt gagtgtgcaa ccaaacggat gtatccagat gttttatctt
    43981 acgataagat tgttgacttg acaggatcct tcaaggtgcc tatgggttgc cgttctttcc
    44041 tccaaggatg gaaggatgaa aatggtgtag aagtcaattc aggtcgcatg aatctgggtg
    44101 ttgtgacggt taatctgcct cgtattgctc ttgagtctga aggtgatatg aataagttct
    44161 gggaaatctt caacgagcga atgaatatcg cagaagatgc tcttgtttac cgtgtcgaac
    44221 gcactaaaga ggcgacacca gcaaatgctc ctattcttta tcagtacggt gcttttggcc
    44281 atcgtctagg taaagaagaa agtgttgacc agctctttaa gaatcgtcgt gctaccgttt
    44341 cgctgggcta tatcggcttg tatgaagtag cgacagtttt ctttggtaac agctgggaaa
    44401 gtaatccaga tgctaaggaa ttcacgctag acatcattca cgatatgaaa cgccgtgtag
    44461 aagagtggtc agaccaatat ggctaccatt tctctatcta ctcaacacca tccgaaagtc
    44521 tgacagaccg tttctgtcga ctagatatag acaagtttgg ctctattcct gatatcacag
    44581 acaaggaata ctacaccaac tctttccact acgatgttcg taaaaatcca acaccgtttg
    44641 aaaaattgga ctttgagaaa gtctatccag aagcaggtgc gtcaggtggt ttcatccatt
    44701 attgtgagta tccagtcctt cagcaaaatc caaaggcctt ggaagctgtc tgggattatg
    44761 cttatgaccg tgtaggctat ctaggcacca atactccgat tgaccgttgc tacaagtgtg
    44821 actttgaagg ggattttgaa ccaactgaga gagggtttgc ttgtccaaac tgtggcaata
    44881 gcgaccctaa aacagtagat gtggtgaagc gaacttgtgg ctacctaggt aatcctcaag
    44941 caagaccgat ggtcaacggc cgtcacaagg aaatcgctgc gcgtgtcaaa catatgaatg
    45001 gttcaacgat taaaatagct gggcatcaag taacaaatta gaaagaaatg aaatgggaaa
    45061 atatcaacta gacgataagg ggcgcgcaca agtgacccgt tatcacgaga aacactctaa
    45121 aggtggagct ggtaagaaag aacgcttgct tagcttcaga gaacaatttt taaacaagaa
    45181 caagaaaaaa taaaagtgag agccagctct cgcttttctc atagtgggag gtaaggatgg
    45241 aattacgcag accaagatta gcggataaga aagctgtttt agatatgatg acagagtttg
    45301 aaaagagcca atcagcccat gatggaggat tttgggatac agagaatttt gtgtatgaag
    45361 agtggttgga aagcaatcag gaacaggaaa tggggattaa tctgcctgaa ggatgggttc
    45421 ctgcaattca gttagtggct ttttctgaga aaggtcaagc agttggattt cttaatctcc
    45481 ggttgcgcct cagtaacttt ttactagaag aaggtgggca cattggctac tccatccgtc
    45541 catctgaaag aggcaagggt tatgcaaaag agactctccg tcagggcttg caagttgcta
    45601 aggaaaagaa catcaagaaa gctctggtga cctgtagcgt gaataatcct gctagcagag
    45661 cagtcattct agcaaatggt ggaatatttg aggatgctca caatggagtc gagcgttatt
    45721 ggatagaggt agcgaatgaa taatccaaaa ccacaagaat ggaaaagcga ggaacttagt
    45781 caaggtcgta tcattgacta caaggccttt aactttgtgg acggcgaagg cgtgcgtaac
    45841 tctctctatg tatcaggctg tatgtttcac tgcgagggat gttataatgt tgcgacttgg
    45901 tcttttaatg ctggcattcc ctatacagca gaattagaag agcagatcat ggcagatctt
    45961 gctcagccct atgttcaagg tttgactttg ttgggagggg agccttttct taatactggg
    46021 attctcttgc cacttgttaa gcggattcgg aaggaattgc cagacaagga catctggtcc
    46081 tggaccggct acacttggga agaaatgatg ttggaaactc cagataaact ggaattcttg
    46141 tcactgattg acattcttgt cgatggaaga tatgatcgaa ctaagagaaa tcttatgctc
    46201 cagtttcgag gttcatctaa ccaacgaatt atcgatgtgc aaaaatcgct caaaagtggg
    46261 caagtagtga tttgggacaa gctcaatgac ggaaaagaaa gctatgaaca ggtgaagaga
    46321 gaatgaagaa aaaagactta gtagaccaac tagtctcaga gatcgagacg gggaaagtca
    46381 ggacactggg aatatacggt catggagctt caggtaaatc aacctttgca caggaattgt
    46441 accaagcttt agattctact acagtaaatt tgctagaaac agatccctat atcacctccg
    46501 gacgctattt ggtagtaccc aaggacgcgc cgaatcaaaa ggtgacagcc agtctgccag
    46561 tggcgcatga actggagagt ttgcagagag atatccttgc cttgcaggcg ggtatggatg
    46621 tcttgacaat tgaagaacct tggaaggcta gtgaggtctt gtctggagcc aaaccaattt
    46681 tgattgtcga agggatgtct gttggttttc tacccaagga actctttgaa aaaaccatct
    46741 gtttctacac ggatgaggag accgaattaa agcgacgcct tgctagagat acgactgtga
    46801 gaaatcgcga tgcatccttt atattggcta gccatcagat gagacgggag cagtatctgc
    46861 gatactataa agaaactgag tctaaggcgg atatcttagt ggaccaatca gaagataaat
    46921 ttgatgtcaa gaggacttaa cttatgtata gaagaaatat agaagaaaat ccctaatttt
    46981 agaaagaaaa agtaggaaaa cagtttcatc ccaaaaaaac gaaaaaacct aacaaatccc
    47041 ttgcaatcgc aggggatttg tgttattcta ttatggtgct gtaaattaca gccttagttt
    47101 tgatgcaaga ggttgcgaca cgctcggttg cattgccacg caacaccgcg tcggttttct
    47161 tgtggagcta gcctattatc ttaaatagac gaaaaggaga aaaagatggc aaacaaaaaa
    47221 atccgtatcc gtttgaaagc ttacgaacac cgtacgcttg acacagcggc tgcaaaaatc
    47281 gtagaatcag ctactcgtac aggtgcacaa gttgcgggtc caatcccact tccaactgaa
    47341 cgtagcctct acacaatcat tcgtgcgact cacaaataca aagactctcg cgaacaattt
    47401 gaaatgcgta cacacaaacg tttgatcgat atcgttaacc caactcaaaa aacagttgat
    47461 gccttgatga aattggatct tccaagtggt gtaaacgtag aaatcaaact ttaatctaaa
    47521 atataaaaga gcagaggctg gtgtttcaat ctaattgaac acgggctaga ctcggtgtga
    47581 aaaagataaa cttcctattg tccgctagac actgcgttag tttcctattt tcactttgag
    47641 tttgacgccc tttgtatctt agacttgagc ataaaaaacg ctcgttaaaa actttttgaa
    47701 taaaaaatat agaaaaggaa ctattttctc atgacaaaag gaatcttagg gaaaaaagtg
    47761 ggaatgactc aaatcttcac tgaagctggc gaattgatcc ctgtaacagt tattgaagca
    47821 actccaaacg ttgttcttca agttaaaact gttgaaacag acggatacaa cgctatccaa
    47881 gttggtttcg atgacaaacg cgaagtattg agcaacaaac ctgctaaagg acatgtagcg
    47941 aaagctaaca cggctcctaa gcgcttcatt cgtgaattca aaaacgttga aggcttggaa
    48001 gttggtgctg aaattacagt tgaaacattc gcagctggag atgttgttga tgtaactggt
    48061 acttctaaag gtaaaggttt ccaaggtgtt atcaagcgcc acggacaatc acgtggaccg
    48121 atggctcacg gttctcgtta ccaccgtcgt ccaggttcta tgggacctgt tgcacctaac
    48181 cgcgtattca aaggtaaaaa ccttgcagga cgtatgggtg gtgaccgcgt aacaattcaa
    48241 aatcttgaag ttgtacaagt tgttccagaa aagaacgtta tccttatcaa aggtaacgta
    48301 ccaggtgcta agaaatctct tatcactatc aaatcagcag ttaaagctgg taaataataa
    48361 agaaagggga aatcagtcac aatggcaaac gtaacattat ttgaccaaac tggtaaagaa
    48421 gctggccaag ttgttcttaa cgatgcagta tttggtatcg aaccaaatga atcagttgtg
    48481 tttgatgtaa tcatcagcca acgcgcaagc cttcgtcaag gaacacacgc tgttaaaaac
    48541 cgctctgcag tatcaggtgg tggacgcaaa ccgtggcgtc aaaaaggaac tggacgtgct
    48601 cgtcaaggtt ctatccgctc accacaatgg cgtggtggtg gtgttgtctt cggaccaact
    48661 ccacgttcat acggctacaa acttccacaa aaagttcgtc gcctagctct taaatcagtt
    48721 tactctgaaa aagttgctga aaacaaattc gtagctgtag acgctctttc atttacagct
    48781 ccaaaaactg ctgaatttgc aaaagttctt gcagcattga gcatcgattc taaagttctt
    48841 gttatccttg aagaaggaaa tgaattcgca gctctttcag ctcgtaacct tccaaacgtg
    48901 aaagttgcaa ctgctacaac tgcaagtgtt cttgacatcg caaatagcga caaacttctt
    48961 gtcacacaag cagctatctc taaaatcgag gaggttcttg cataatgaat ttgtatgatg
    49021 ttatcaaaaa acctgtcatc actgaaagct caatggctca acttgaagca gggaaatatg
    49081 tatttgaagt tgacactcgt gcacacaaac ttttgatcaa gcaagctgtt gaagctgctt
    49141 tcgaaggtgt taaagttgcc aatgttaaca caatcaacgt aaaaccaaaa gctaaacgtg
    49201 ttggacgtta cactggtttt actaacaaaa ctaaaaaagc tatcatcaca cttacagctg
    49261 attctaaagc aatcgagttg tttgctgctg aagctgaata atctaaggag gaaatatcgt
    49321 gggaattcgt gtttataaac caacaacaaa cggtcgccgt aatatgactt ctttggattt
    49381 cgctgaaatc acaacaagca ctcctgaaaa atcattgctt gttgcattga agagcaaggc
    49441 tggtcgtaac aacaacggtc gtatcacagt tcgtcaccaa ggtggtggac acaaacgttt
    49501 ctaccgtttg gttgacttca aacgtaataa agacaacgtt gaagcagttg ttaaaacaat
    49561 cgagtacgat ccaaaccgtt ctgcaaacat cgctcttgta cactacactg acggtgtgaa
    49621 agcatacatc atcgctccaa aaggtcttga agtaggtcaa cgtatcgttt caggtccaga
    49681 agcagatatc aaagtcggaa acgctcttcc acttgctaac atcccagttg gtactttgat
    49741 tcacaacatc gagttgaaac caggtcgtgg tggtgaattg gtacgtgctg ctggtgcatc
    49801 tgctcaagta ttgggttctg aaggtaaata cgttcttgtt cgtcttcaat caggtgaagt
    49861 tcgtatgatt cttggaactt gccgtgctac agttggtgtt gtcggaaacg aacaacatgg
    49921 acttgtaaac cttggtaaag caggacgtag ccgttggaaa ggtatccgcc caacagttcg
    49981 tggttctgta atgaacccta acgatcaccc acacggtggt ggtgaaggta aagcaccagt
    50041 tggtcgtaaa gcaccatcta ctccatgggg caaacctgct cttggtctta aaactcgtaa
    50101 caagaaagcg aaatctgaca aacttatcgt tcgtcgtcgc aacgagaaat aatattaaac
    50161 tagtcgctta agcaactagt aaatccgcca gctcggtagc gctccatagg agtgcaagcc
    50221 gctgtggtac aacatttaaa ggagaaaata taaaaatggg acgcagtctt aaaaaaggac
    50281 ctttcgtcga tgagcatttg atgaaaaaag ttgaagctca agctaacgac gaaaagaaaa
    50341 aagttattaa aacttggtca cgtcgttcaa cgatcttccc aagtttcatt ggttacacta
    50401 ttgcagttta tgacggacgt aaacacgtac ctgtttacat ccaagaagac atggtaggcc
    50461 acaaacttgg tgaatttgca ccaactcgta cttacaaagg tcacgctgca gacgacaaga
    50521 aaacacgtag aaaataagga gaacataaat ggcagaaatt acttcagcta aagcaatggc
    50581 tcgtacagta cgtgtttcac ctcgtaaatc acgtcttgtt cttgataaca tccgtggtaa
    50641 aagcgtagcc gatgcaatcg caatcttgac attcactcca aacaaagctg ctgaaatcat
    50701 cttgaaagtt ttgaactcag ctgtagctaa cgctgaaaac aactttggtt tggataaagc
    50761 taacttggta gtatctgaag cattcgcaaa cgaaggacca actatgaaac gtttccgtcc
    50821 acgtgcgaaa ggttcagctt caccaatcaa caaacgtaca gctcacatca ctgtagctgt
    50881 tgcagaaaaa taaggaggta aaatcgtggg tcaaaaagta catccaattg gtatgcgtgt
    50941 cggcatcatc cgtgattggg atgccaaatg gtatgctgaa aaagaatacg cggattacct
    51001 tcatgaagat cttgcaatcc gtaaattcgt tcaaaaagaa cttgctgacg cagcagtttc
    51061 aactattgaa atcgaacgcg cagtaaacaa agttaacgtt tcacttcaca ctgctaaacc
    51121 aggtatggtt atcggtaaag gtggtgctaa cgttgatgca ctccgtgcaa aacttaacaa
    51181 attgactgga aaacaagtac acatcaacat catcgaaatc aaacaacctg atttggatgc
    51241 tcaccttgta ggtgaaggaa ttgctcgtca attggagcaa cgtgttgctt tccgtcgtgc
    51301 acaaaaacaa gcaatccaac gtgcaatgcg tgctggagct aaaggaatca aaactcaagt
    51361 atcaggtcgt ttgaacggtg cagatatcgc ccgtgctgaa ggatactctg aaggaactgt
    51421 tccgcttcac acacttcgtg cagatatcga ttacgcttgg gaagaagcag atactacata
    51481 cggtaaactt ggtgttaaag tatggatcta ccgtggtgaa gttcttccag ctcgtaaaaa
    51541 cactaaagga ggtaaataac caatgttagt acctaaacgt gttaaacacc gtcgtgagtt
    51601 ccgtggaaaa atgcgcggtg aagcaaaagg tggaaaagaa gtagcattcg gtgaatacgg
    51661 tcttcaagct acaactagcc actggatcac taaccgccaa atcgaagctg ctcgtatcgc
    51721 catgactcgt tacatgaaac gtggtggtaa agtttggatt aaaatcttcc cacacaaatc
    51781 atacactgct aaagctatcg gtgtgcgtat gggatctggt aaaggggcac ctgaaggttg
    51841 ggtagcacca gttaaacgtg gtaaagtgat gttcgaaatc gctggtgtat ctgaagagat
    51901 tgcacgtgaa gcgcttcgac ttgctagcca caaattgcca gttaaatgta aattcgtaaa
    51961 acgtgaagca gaataaggag aaggcatgaa acttaatgaa gtaaaagaat ttgttaaaga
    52021 acttcgtggt ctttctcaag aagaactcgc gaagcgcgaa aacgaattga aaaaagaatt
    52081 gtttgaactt cgtttccaag ctgctactgg tcaattggaa caaacagctc gcttgaaaga
    52141 agttaaaaaa caaatcgctc gtatcaaaac agttcaatct gaagcgaaat aatagactag
    52201 ggaaggagaa atttcaatgg aacgcaataa tcgtaaagtt cttgttggac gtgttgtatc
    52261 tgacaaaatg gacaagacaa tcacagttgt agttgaaaca aaacgtaacc acccagtcta
    52321 tggtaaacgt attaactact ctaaaaaata caaagctcat gatgaaaaca atgttgccaa
    52381 agaaggcgat atcgtacgta tcatggaaac tcgcccgctt tcagctacaa aacgtttccg
    52441 tcttgtagaa gttgttgaag aagcggtcat catctaatca aacctgaaag gagaaaactg
    52501 aaatgattca aacagaaact cgtttgaaag tcgcagacaa cagcggtgct cgcgaaatct
    52561 tgactatcaa agttcttggt ggttcaggac gtaaatttgc aaacatcggt gatgttatcg
    52621 tggcatctgt aaaacaagct actcctggtg gtgcggttaa aaaaggtgac gttgttaaag
    52681 cagttatcgt tcgtactaaa tcaggtgctc gtcgtgctga tggttcatac atcaaatttg
    52741 acgaaaacgc agcagttatc atccgtgaag acaaaactcc tcgcggaaca cgtatctttg
    52801 gcccagttgc acgtgaattg cgtgaaggtg gcttcatgaa gatcgtgtca cttgctccag
    52861 aagtacttta atttttagga acaaactagt cccctagctt caagctaggg tgcccttatg
    52921 ggcgtaagaa aaatcaagga gaaacctaat gtttgtaaaa aaaggcgaca aagttcgcgt
    52981 aatcgctggt aaagataagg gaacagaagc tgttgtcctt actgcccttc caaaagtaaa
    53041 caaagttatc gttgaaggtg ttaacattgt taagaaacac caacgtccaa ctaacgagct
    53101 tcctcaaggt ggtatcatcg agaaagaagc agctatccac gtatcaaacg ttcaagtttt
    53161 ggacaaaaat ggtgtagctg gtcgtgttgg atacaaattt gtagacggta aaaaagttcg
    53221 ctacaacaaa aaatcaggcg aagtgcttga ttaatcacga aggaaaggag aagtataatg
    53281 gcaaatcgtt taaaagaaaa atatcttaat gaagtagttc ctgctttgac agaacaattc
    53341 aactactcat cagtgatggc tgtgcctaaa gtagataaga ttgttttgaa catgggtgtt
    53401 ggtgaagctg tatcaaacgc taaaagcctt gaaaaagctg ctgaagaatt ggcacttatc
    53461 tcaggtcaaa aaccacttat cactaaagct aaaaaatcaa tcgccggctt ccgtcttcgt
    53521 gaaggtgttg cgatcggtgc aaaagttacc cttcgtggtg aacgtatgta cgaattcttg
    53581 gataaattgg tatcagtttc acttccacgt gtacgtgact ttcacggtgt tccaacaaaa
    53641 tcatttgatg gacgcgggaa ctacacactt ggtgtgaaag aacaattaat cttcccagaa
    53701 atcaacttcg atgacgttga caaaactcgt ggtcttgaca tcgttatcgt aacaactgct
    53761 aacactgacg aagagtcacg tgcattgctt acaggccttg gaatgccttt tgcaaaataa
    53821 tataggaggt aaatctaatg gctaaaaaat caatggtagc tagagaggct aaacgccaaa
    53881 aaattgttga ccgttatgct gaaaaacgtg ctgcattaaa ggcggcaggg gactacgaag
    53941 gtttatctaa attacctcgc aacgcctcac cgactcgttt acataatcgt tgtagagtta
    54001 cggggcgccc acattcagtt taccgcaaat ttggtctgag tcgtatcgct tttcgcgaac
    54061 ttgcgcataa aggtcaaatt cctggtgtaa caaaagcatc ttggtaattt aagatatcaa
    54121 gggtgtcaaa actccaagta aaaataggaa acttgacgaa gaaactaaag tttctaggaa
    54181 agtttatctt tttcacacag agtttagccc gggttcaatt gggcttgcca atttgaaccc
    54241 gagctacagc tttggcaaaa aagaccaatt tgctttggag cattgcttct gcattaaatt
    54301 gtctattttt gctcgtgctg ttacgctctt tgtatcatgt attaactagc aagtgcaact
    54361 tgcaaactac tagtaagagg agaaaaacaa aatggttatg actgacccaa tcgcagactt
    54421 cctaactcgt attcgtaatg ctaaccaagc taaacacgaa gtacttgaag tacctgcatc
    54481 aaacatcaaa aaagggattg ctgaaatcct taaacgcgaa ggttttgtaa aaaacgttga
    54541 aatcatcgaa gatgacaaac aaggcgtcat ccgtgtattt cttaaatacg gaccaaatgg
    54601 tgaaaaagtt atcactaact tgaaacgtgt ttctaaacca ggacttcgtg tctacaaaaa
    54661 acgtgaagac cttccaaaag ttcttaacgg acttggaatt gccatccttt caacttctga
    54721 aggtttgctt actgataaag aagcacgcca aaagaatgtt ggtggtgagg ttatagctta
    54781 cgtttggtaa aatcaagata caaagctcgt aaagaacaaa gcaaaattag gaagttggag
    54841 aagtttgttt acaaacaagc caacttatct attttgcaca gttcttagag cgtgttcagt
    54901 tcagctcttg aactaaataa gtatctgaac cccgtgaaaa ctggccgttc tggcctgaca
    54961 atttaacagg agaaaataaa catgtcacgt attggtaata aagttatcgt gttgcctgct
    55021 ggtgttgaac tcgctaacaa tgacaacgtt gtaactgtaa aaggacctaa aggagaactt
    55081 actcgtgagt tctcaaaaga tattgaaatc cgtgtggaag gtactgaaat aactcttcac
    55141 cgtccaaacg attcaaaaga aatgaaaact atccacggaa ctactcgtgc ccttttgaac
    55201 aacatggttg ttggtgtatc agaaggattc aagaaagaac ttgaaatgcg tggggttggt
    55261 taccgtgcac agcttcaagg atctaaactt gttttggctg ttggtaaatc tcatccagac
    55321 gaagttgaag ctccagaagg aattactttt gaacttccaa acccaacaac aatcgttgtt
    55381 agcggaattt caaaagaagt agttggtcaa acagctgctt acgtacgtag ccttcgttca
    55441 ccagaaccat ataaaggtaa aggtatccgt tacgttggtg aattcgttcg ccgtaaagaa
    55501 ggtaaaacag gtaaataatg ttgagtggtt gatcatcaac caccaaccta ttttccaact
    55561 ttgtgcatag cacacgattt aaaactaaag aggtgaaaac tgtgatttca aaaccagata
    55621 aaaacaaact ccgccaaaaa cgccaccgtc gcgttcgcgg aaaactctct ggaactgctg
    55681 atcgcccacg tttgaacgta ttccgttcta atacaggcat ctacgctcaa gtgattgatg
    55741 acgtagcggg tgtaacgctc gcaagtgctt caactcttga taaagaagtt tcaaaaggaa
    55801 ctaaaactga acaagccgtt gctgtcggta aactcgttgc agaacgtgca aacgctaaag
    55861 gtatttcaga agtggtgttc gaccgcggtg gatatctata tcacggacgt gtgaaagctt
    55921 tggctgatgc agctcgtgaa aacggattga aattctaata ggaggacact agaaaatggc
    55981 atttaaagac aatgcagttg aattagaaga acgcgtagtt gctgtcaacc gtgttacaaa
    56041 agttgttaaa ggtggacgtc gtcttcgttt cgcagctctt gttgttgttg gtgaccacaa
    56101 tggtcgcgta ggatttggta ctggtaaagc tcaagaagtt ccagaagcaa tccgtaaagc
    56161 agtagatgat gctaagaaaa acttgatcga agttcctatg gttggaacaa caatcccaca
    56221 cgaagttctt tcagaattcg gtggagctaa agtattgttg aaacctgctg tagaaggttc
    56281 tggagttgcc gctggtggtg cagttcgtgc cgttgtggaa ttggcaggtg tggcagatat
    56341 tacatctaaa tcacttggtt ctaacactcc aatcaacatt gttcgtgcaa ctgttgaagg
    56401 tttgaaacaa ttgaaacgcg ctgaagaaat tgctgccctt cgtggtattt cagtttctga
    56461 tttggcataa gaaaggggat aaaatggctc aaattaaaat tactttgact aagtctccaa
    56521 tcggacgcat tccatcacaa cgtaaaactg ttgtagcact tggacttggc aaattgaaca
    56581 gctctgttat taaagaagat aacgctgcta tccgtggtat gattacagca gtatctcact
    56641 tagtaacagt tgaagaagta aactaatgaa gttttagggg atgtgcactg taccatcccc
    56701 taaaactaga tatagtcatc tatgatgaca tcgtataggc gagttgatgg gggagacaac
    56761 cttttctccc ttatcggcgc tagcatttta caaaagagga gaaaataaaa atgaaacttc
    56821 atgaattgaa acctgcagaa ggttctcgta aagtacgtaa ccgcgttggt cgtggtactt
    56881 catcaggtaa cggtaaaaca tctggtcgtg gtcaaaaagg tcaaaaagct cgtagcggtg
    56941 gcggagttcg ccttggtttt gaaggtggac aaactccatt gttccgtcgt cttccaaaac
    57001 gtggattcac taacatcaac gctaaagaat acgcaattgt gaaccttgac caattgaacg
    57061 tctttgaaga tggtgctgaa gtaactccag ttgttcttat cgaagcagga attgttaaag
    57121 ctgaaaagtc aggtattaaa attcttggta acggtgagtt gactaagaaa ttgactgtga
    57181 aagcagctaa attctctaaa tcagctgaag aagctatcac tgctaaaggt ggttcagtag
    57241 aagtcatcta agagaggtga cctatgtttt ttaaattatt aagagaagct cttaaagtca
    57301 agcaggttcg atcaaaaatt ttatttacaa tttttatcgt tttggtcttt cgtatcggaa
    57361 ctagcattac agttcctggt gtgaatgcca atagcttgaa tgctttaagt ggattatcct
    57421 tcttaaacat gttgagcttg gtgtcgggga atgccctaaa aaacttttcg atttttgccc
    57481 taggagttag tccctatatc accgcttcta ttgttgtcca actcttgcaa atggatattt
    57541 tacccaagtt tgtagagtgg ggtaaacaag gggaagtagg tcgaagaaaa ttgaatcaag
    57601 ctactcgtta tattgctcta gttctcgctt ttgtgcaatc tatcgggatt acagctggtt
    57661 ttaatacctt ggctggagct caattgatta aaactgcttt aactccacaa gtttttctga
    57721 cgattggtat catcttaaca gctggtagta tgattgtcac ttggttgggt gagcaaatta
    57781 cagataaggg atacggaaac ggtgtttcca tgattatctt tgccgggatt gtttcctcaa
    57841 ttccagagat gattcagggc atctatgtgg actactttgt gaacgtccca agtagccgta
    57901 tcacttcatc tatcattttc gtaatcattt tgattattac tgtattgttg attatttact
    57961 ttacaactta tgttcaacaa gcagaataca aaattccaat ccaatatact aaggttgcac
    58021 aaggtgctcc atctagctct taccttccgt taaaggtaaa ccctgctgga gttatccctg
    58081 ttatctttgc cagttcgatt actgcagcgc ctgcggctat tcttcagttt ttgagtgcca
    58141 caggtcatga ttgggcttgg gtaagggtag cacaagagat gttggcaact acttctccaa
    58201 ctggtattgc catgtatgct ttgttgatta ttctctttac attcttctat acgtttgtac
    58261 agattaatcc tgaaaaagca gcagagagcc tacaaaagag tggtgcctat atccatggag
    58321 ttcgtcctgg taaaggtaca gaagaatata tgtctaaact tcttcgtcgt cttgcaactg
    58381 ttggttccct cttccttggt gtgatttcca ttttaccgat tgcagctaaa gatgtatttg
    58441 gtctttctga tgttgttgcc tttggtggaa caagtctctt gatcattatc tctacaggta
    58501 tcgaaggaat caagcaattg gaaggttacc tattgaaacg taagtatgtt ggtttcatgg
    58561 acagaacaga ataaaagtat ttactgaatc agtaaatact gagggagtgg aggtttaaac
    58621 tctgacattt gtaagagttg gatctcccct cttctatttt gtttttaaat cggggtgaaa
    58681 agactttttg cttctattta aaaataaaat aaggagatca aatcatgaat cttttgatta
    58741 tgggcttacc tggtgcaggt aagggaactc aagcagcaaa aatcgtagaa caattccatg
    58801 ttgcacatat ctcaacaggt gatatgttcc gcgctgcaat ggcaaatcaa actgaaatgg
    58861 gtgttcttgc taagtcatat attgacaagg gtgaattggt tcctgacgaa gttacaaatg
    58921 gaatcgtaaa agaacgcctt tcacaagatg atattaaaga aacaggattc ttattggatg
    58981 gttacccacg tacaattgaa caagctcatg ccttggacaa aacattggct gaacttggca
    59041 ttgaactaga aggtattatc aatattgaag tgaaccctga cagcctcttg gaacgtttga
    59101 gtggccgtat catccaccgc gtaactggag aaactttcca caaggtcttt aacccaccag
    59161 ttgactataa agaagaagat tactaccaac gtgaagatga taagcctgag acagtaaaac
    59221 gtcgtttgga tgttaatatt gctcaaggag aaccaatcat tgctcactac cgtgccaaag
    59281 gtttggttca tgacatcgaa ggtaatcaag atatcaatga tgtcttctca gatattgaaa
    59341 aagtattgac aaatttgaaa taaagcgttt ttcacacttg caaaaatccg ctacaaatgt
    59401 tatactgaaa tagtctgact tataattgtt gtctctgtgt ctagaggcat cgaatcgaaa
    59461 tttatggagg tgcttttgcg tggcaaaaga cgatgtgatt gaagttgaag gcaaagtagt
    59521 tgatacaatg ccgaatgcaa tgtttacggt tgaacttgaa aatggacatc agattttagc
    59581 aacagtttct ggtaaaattc gtaaaaacta tattcgtatt ttagcgggag atcgtgttac
    59641 tgtcgaaatg agtccatatg acttgacacg tggacgtatc acttaccgct ttaaataatc
    59701 gaaaaacttg gagggataag aaatgaaagt aagaccatcg gtcaaaccaa tttgcgaata
    59761 ctgtaaagtt attcgtcgta atggtcgtgt tatggtaatt tgcccagcaa atccaaaaca
    59821 caaacaacgt caaggataag atagaaagga gaaaacatgg ctcgtattgc tggagttgat
    59881 attccaaatg acaaacgcgt agtaatctca ttgacttatg tttatggtat cggacttgca
    59941 acatctaaga aaattttggc tgctgctgga atctcagaag atgttcgtgt acgtgatctt
    60001 acatcagatc aagaagatgc tatccgtcgt gaagtggatg caatcaaagt tgaaggtgac
    60061 cttcgtcgtg aagtaaactt gaacatcaaa cgtttgatgg aaatcggttc ataccgtggt
    60121 atccgtcacc gtcgtggact tcctgtccgt ggacaaaata ctaaaaacaa cgctcgcact
    60181 cgtaaaggta aagctgttgc gattgctggt aagaaaaaat aatataggag gtaaaagtct
    60241 tggctaaacc aacacgtaaa cgtcgtgtga aaaagaatat cgaatctggt attgctcata
    60301 ttcacgctac atttaataac actattgtta tgattactga tgtgcatggt aatgcaattg
    60361 cttggtcatc agctggtgct cttggtttca aaggttctcg taaatctaca ccattcgctg
    60421 ctcaaatggc ttctgaagct gctgctaaat ctgcacaaga acacggtctt aaatcagttg
    60481 aagttactgt aaaaggtcca ggttctggtc gtgagtcagc tattcgtgcg cttgctgccg
    60541 ctggtcttga agtaacagca attcgtgatg tgactccagt gccacacaat ggtgctcgtc
    60601 ctccaaaacg tcgccgtgta taatcatcgc attacactgc ttttcgttta agagggagta
    60661 actaaatgat cgagtttgaa aaaccaaata taacaaaaat tgatgaaaat aaagattatg
    60721 gcaagtttgt aatcgaacca cttgaacgtg gctacggtac aactcttggt aactctcttc
    60781 gtcgtgtact tctagcttct ctaccaggag cagctgtgac atctatcaac attgatggtg
    60841 tgttacatga gtttgacaca gttccaggtg ttcgtgaaga cgttatgcaa atcattctga
    60901 acattaaagg aattgcagtg aaatcgtacg ttgaagacga aaaaatcatc gaactggatg
    60961 ttgaaggtcc tgctgaagta acagctggtg acattttgac agatagcgat attgaaattg
    61021 taaatccaga tcattatctc tttacaattg gtgaaggttc ttctctaaaa gcgactatga
    61081 ctgttaacag tggtcgtgga tatgtacctg ctgatgaaaa taaaaaggat aatgcaccag
    61141 ttggaacact tgctgtagat tctatttata caccagttac aaaagtcaac tatcaagtgg
    61201 aacctgctcg tgtaggtagc aatgatggtt tcgacaaatt aacccttgaa atcttgacaa
    61261 atggaacaat tattccagaa gatgctttag ggctttcagc acgtattttg acagaacatc
    61321 ttgatttgtt tacaaatctt actgagattg ctaagtcaac tgaagtgatg aaagaagctg
    61381 atactgaatc tgacgaccgt attttagatc gtacgattga ggaactggac ttgtctgtgc
    61441 gttcatacaa ctgtttaaaa cgtgccggta tcaatactgt gcatgatttg acagaaaaat
    61501 ctgaagcaga gatgatgaaa gtacgaaatc ttggacgcaa gagtttggaa gaagtgaaac
    61561 tcaaactcat tgatttgggt cttggattaa aagataaata aaggaggaat acatggctta
    61621 ccgtaaacta ggacgcacta gctcacaacg taaagcaatg cttcgcgatt tgacaactga
    61681 ccttttgatc aacgaatcaa tcgtgacaac tgaagctcgt gctaaagaaa tccgtaaaac
    61741 tgttgaaaaa atgattactc taggtaaacg tggtgatttg catgcacgtc gtcaagcagc
    61801 tgctttcgta cgtaatgaaa tcgcatctga aaactatgat gaagcaactg ataagtacac
    61861 ttctactaca gcacttcaaa aattgttctc agaaatcgca cctcgttatg ctgaacgtaa
    61921 cggtggatac actcgtatcc ttaaaactga accacgtcgt ggtgatgcag cgccaatggc
    61981 gatcatcgaa ttagtataaa atcatcaatt ttgttgagtg ttatgatgat ggagtcttgt
    62041 gctcttagtc tagctctggt ctaccgctag gatttcggtc ctagcgggaa cactcatcat
    62101 aagttgggat agtagacgct tgtttacgaa attgtttttt tcttaagaac aacttcgtaa
    62161 gcaggcgttt ttgagtattt tcgttagaat tatgctatac tatttgaaaa gaatcctgtt
    62221 taatgttaag gtttcttatt ttaagaagaa ttggagttta cttatgaaag ccattataac
    62281 tgttgttggt aaagataaat ctggaattgt tgcaggtgtt tctggtaaaa ttgcagaatt
    62341 aggattgaat attgacgata tctctcaaac tgtattggat gaatatttta cgatgatggc
    62401 tgttgtatct agtgatgaaa agcaagattt tacctatctt cgtaatgaat ttgaagcttt
    62461 tgggcaaact ttgaatgtaa aaatcaatat tcagagtgca gcgattttcg aagctatgta
    62521 taatatctag gaggtcatca tggatattag acaagttact gaaaccatcg ccatgattga
    62581 ggagcaaaac ttcgatatta gaaccattac catggggatt tctcttttgg actgtatcga
    62641 tccaaatatc aatcgtgctg cggagaaaat ctatcaaaaa attacgacaa aggcggctaa
    62701 tttagtagct gttggtgatg aaattgcggc tgagttggga attcctatcg ttaataagcg
    62761 tgtatcggtg acacctattt ctctgattgg ggcagcgaca gatgcgacgg actacgtggt
    62821 tctggcaaaa gcgcttgata aggctgcgaa agagattggt gtggacttta ttggtggttt
    62881 ttctgcctta gtacaaaaag gttatcaaaa gggagatgag attctcatca attccattcc
    62941 tcgcgctttg gctgagacgg ataaggtctg ctcgtcagtc aatatcggct caaccaagtc
    63001 tggtattaat atgacggctg tggcagatat gggacgaatt atcaaggaaa cagcaaatct
    63061 ttcagatatg ggagtggcca agttggttgt attcgctaat gctgttgagg acaatccatt
    63121 tatggcgggt gcctttcatg gtgttgggga agcagatgtt atcatcaatg tcggagtttc
    63181 tggtcctggt gtggtgaaac gtgctttgga aaaagttcgt ggacagagct ttgatgtagt
    63241 agccgaaaca gttaagaaaa ctgcctttaa aatcactcgt atcggtcaat tggttggtca
    63301 aatggccagt gagagactgg gtgtggagtt tggtattgtg gacttgagtt tggcaccaac
    63361 ccctgcggtt ggagactctg tagcacgtgt ccttgaggaa atggggctag aaacagttgg
    63421 cacgcatgga acgacggctg ccttggccct cttgaacgac caagttaaaa agggtggagt
    63481 gatggcctgc aaccaagtcg gtggtttatc tggtgccttt atccctgttt ctgaggatga
    63541 aggaatgatt gctgcagtgc aaaatggctc tcttaattta gaaaaactag aagctatgac
    63601 ggctatctgt tctgttggat tggatatgat tgccatccca gaagatacgc ctgctgaaac
    63661 cattgcggct atgattgcgg atgaagcagc aatcggtgtt atcaacatga aaacaacagc
    63721 tgttcgtatc attcccaaag gaagagaagg cgatatgatt gagtttggtg gtctattagg
    63781 aactgcacct gttatgaagg ttaatggggc ttcgtctgtc gacttcatct ctcgcggtgg
    63841 acaaatccca gcaccaattc atagttttaa aaattaagaa aataggagaa attttaagtt
    63901 ctatttaaga t
//
LOCUS       DAVKLX010000005        61812 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKLX010000005 DAVKLX010000000
VERSION     DAVKLX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 61812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61812
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.005"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(115..1476)
                     /gene="dnaA"
                     /locus_tag="VW325_000353"
     CDS             complement(115..1476)
                     /gene="dnaA"
                     /locus_tag="VW325_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9733822.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(1689..2447)
                     /locus_tag="VW325_000354"
     CDS             complement(1689..2447)
                     /locus_tag="VW325_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9733823.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(2505..3686)
                     /locus_tag="VW325_000355"
     CDS             complement(2505..3686)
                     /locus_tag="VW325_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9733824.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            3869..4348
                     /gene="rlmH"
                     /locus_tag="VW325_000356"
     CDS             3869..4348
                     /gene="rlmH"
                     /locus_tag="VW325_000356"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9733825.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            4392..4465
                     /locus_tag="VW325_000357"
     tRNA            4392..4465
                     /locus_tag="VW325_000357"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4426..4428,aa:Arg,seq:tct)
     gene            4631..4756
                     /gene="comC"
                     /locus_tag="VW325_000358"
     CDS             4631..4756
                     /gene="comC"
                     /locus_tag="VW325_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9733826.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            4777..6102
                     /locus_tag="VW325_000359"
     CDS             4777..6102
                     /locus_tag="VW325_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9733827.1"
                     /translation="MDLFGFGTVIVHLLIISHNYHLICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIAIFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLPIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFS
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            6099..6851
                     /locus_tag="VW325_000360"
     CDS             6099..6851
                     /locus_tag="VW325_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9733828.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQFIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            6903..6974
                     /locus_tag="VW325_000361"
     tRNA            6903..6974
                     /locus_tag="VW325_000361"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6936..6938,aa:Glu,seq:ttc)
     gene            6980..7053
                     /locus_tag="VW325_000362"
     tRNA            6980..7053
                     /locus_tag="VW325_000362"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7014..7016,aa:Asn,seq:gtt)
     gene            complement(7094..7636)
                     /locus_tag="VW325_000363"
     CDS             complement(7094..7636)
                     /locus_tag="VW325_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU9733829.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            7815..8582
                     /locus_tag="VW325_000364"
     CDS             7815..8582
                     /locus_tag="VW325_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU9733830.1"
                     /translation="MIGISLIPALYNIIFLSSMWEPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQGLQGSLENLQNQSSNLST
                     LKNQANQVLPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            8586..9005
                     /locus_tag="VW325_000365"
                     /pseudo
     CDS             8586..9005
                     /locus_tag="VW325_000365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9033..9347
                     /locus_tag="VW325_000366"
     CDS             9033..9347
                     /locus_tag="VW325_000366"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU9733831.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(9448..12000)
                     /locus_tag="VW325_000367"
     CDS             complement(9448..12000)
                     /locus_tag="VW325_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9733832.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSNLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQILKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLNRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(12062..13684)
                     /locus_tag="VW325_000368"
     CDS             complement(12062..13684)
                     /locus_tag="VW325_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9733833.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEIQAKVKELWKD"
     gene            13890..14915
                     /gene="trpS"
                     /locus_tag="VW325_000369"
     CDS             13890..14915
                     /gene="trpS"
                     /locus_tag="VW325_000369"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9733834.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            15067..16545
                     /gene="guaB"
                     /locus_tag="VW325_000370"
     CDS             15067..16545
                     /gene="guaB"
                     /locus_tag="VW325_000370"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9733835.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(16596..17693)
                     /gene="recF"
                     /locus_tag="VW325_000371"
     CDS             complement(16596..17693)
                     /gene="recF"
                     /locus_tag="VW325_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9733836.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(17696..18064)
                     /gene="yaaA"
                     /locus_tag="VW325_000372"
     CDS             complement(17696..18064)
                     /gene="yaaA"
                     /locus_tag="VW325_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU9733837.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            18223..19473
                     /locus_tag="VW325_000373"
     CDS             18223..19473
                     /locus_tag="VW325_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9733838.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            19470..20753
                     /locus_tag="VW325_000374"
     CDS             19470..20753
                     /locus_tag="VW325_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9733839.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            20795..21616
                     /locus_tag="VW325_000375"
     CDS             20795..21616
                     /locus_tag="VW325_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9733840.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKISFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            21627..22172
                     /gene="pgsA"
                     /locus_tag="VW325_000376"
     CDS             21627..22172
                     /gene="pgsA"
                     /locus_tag="VW325_000376"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9733841.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            22169..22996
                     /locus_tag="VW325_000377"
     CDS             22169..22996
                     /locus_tag="VW325_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9733842.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            22981..23820
                     /locus_tag="VW325_000378"
     CDS             22981..23820
                     /locus_tag="VW325_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9733843.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            23813..24607
                     /locus_tag="VW325_000379"
     CDS             23813..24607
                     /locus_tag="VW325_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9733844.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            24666..25484
                     /gene="mreC"
                     /locus_tag="VW325_000380"
     CDS             24666..25484
                     /gene="mreC"
                     /locus_tag="VW325_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9733845.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            25484..25978
                     /gene="mreD"
                     /locus_tag="VW325_000381"
     CDS             25484..25978
                     /gene="mreD"
                     /locus_tag="VW325_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9733846.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            26072..27250
                     /locus_tag="VW325_000382"
     CDS             26072..27250
                     /locus_tag="VW325_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9733847.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            27474..28253
                     /gene="rpsB"
                     /locus_tag="VW325_000383"
     CDS             27474..28253
                     /gene="rpsB"
                     /locus_tag="VW325_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9733848.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            28332..29372
                     /locus_tag="VW325_000384"
     CDS             28332..29372
                     /locus_tag="VW325_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9733849.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            29619..29942
                     /gene="tnpB"
                     /locus_tag="VW325_000385"
     CDS             29619..29942
                     /gene="tnpB"
                     /locus_tag="VW325_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9733850.1"
                     /translation="MIIQLSDLGQVHLVCSKIDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENVRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
     gene            complement(30097..30405)
                     /locus_tag="VW325_000386"
                     /pseudo
     CDS             complement(30097..30405)
                     /locus_tag="VW325_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            30396..30794
                     /locus_tag="VW325_000387"
     CDS             30396..30794
                     /locus_tag="VW325_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9733851.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(30991..31911)
                     /gene="cysK"
                     /locus_tag="VW325_000388"
     CDS             complement(30991..31911)
                     /gene="cysK"
                     /locus_tag="VW325_000388"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9733852.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(32196..32831)
                     /locus_tag="VW325_000389"
     CDS             complement(32196..32831)
                     /locus_tag="VW325_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9733853.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            32887..34185
                     /locus_tag="VW325_000390"
     CDS             32887..34185
                     /locus_tag="VW325_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9733854.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILAEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            34182..34844
                     /locus_tag="VW325_000391"
     CDS             34182..34844
                     /locus_tag="VW325_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9733855.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            34924..35472
                     /gene="raiA"
                     /locus_tag="VW325_000392"
     CDS             34924..35472
                     /gene="raiA"
                     /locus_tag="VW325_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9733856.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            35608..37581
                     /locus_tag="VW325_000393"
     CDS             35608..37581
                     /locus_tag="VW325_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9733857.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            37578..38030
                     /gene="rplI"
                     /locus_tag="VW325_000394"
     CDS             37578..38030
                     /gene="rplI"
                     /locus_tag="VW325_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9733858.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            38074..39426
                     /gene="dnaB"
                     /locus_tag="VW325_000395"
     CDS             38074..39426
                     /gene="dnaB"
                     /locus_tag="VW325_000395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9733859.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            39428..39694
                     /locus_tag="VW325_000396"
     CDS             39428..39694
                     /locus_tag="VW325_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733860.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            39783..41129
                     /gene="cbpD"
                     /locus_tag="VW325_000397"
     CDS             39783..41129
                     /gene="cbpD"
                     /locus_tag="VW325_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9733861.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      41212..41314
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            41385..41675
                     /locus_tag="VW325_000398"
     CDS             41385..41675
                     /locus_tag="VW325_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9733862.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETPH"
     gene            41638..42396
                     /locus_tag="VW325_000399"
     CDS             41638..42396
                     /locus_tag="VW325_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9733863.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            42434..43441
                     /locus_tag="VW325_000400"
     CDS             42434..43441
                     /locus_tag="VW325_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9733864.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            43441..44169
                     /locus_tag="VW325_000401"
     CDS             43441..44169
                     /locus_tag="VW325_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9733865.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            44288..44746
                     /locus_tag="VW325_000402"
     CDS             44288..44746
                     /locus_tag="VW325_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9733866.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRDLLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            44748..47180
                     /locus_tag="VW325_000403"
     CDS             44748..47180
                     /locus_tag="VW325_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9733867.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            47501..48154
                     /gene="rr06"
                     /locus_tag="VW325_000404"
     CDS             47501..48154
                     /gene="rr06"
                     /locus_tag="VW325_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9733868.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            48151..49482
                     /gene="hk06"
                     /locus_tag="VW325_000405"
     CDS             48151..49482
                     /gene="hk06"
                     /locus_tag="VW325_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9733869.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            49565..50092
                     /locus_tag="VW325_000406"
     CDS             49565..50092
                     /locus_tag="VW325_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9733870.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            50171..52243
                     /gene="pspC"
                     /locus_tag="VW325_000407"
     CDS             50171..52243
                     /gene="pspC"
                     /locus_tag="VW325_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEU9733871.1"
                     /translation="MFASKSERKVHYSIRKFSVGVASVVVASLFLGGVVHAEGVRSGD
                     TPKVTSSGQDISKKYADEVKSHLEKILSEIQLDKRKHTQNLAFNKKLSRIQTEYFYLK
                     KKLKAELTSKTKEELTSKTEELTSKTKKELDAAFEQFKKDTLSTEPEKKVAEAEKKVA
                     EAKKKAEDQKEEDRRNYPTNTYKTLELEIAEFDVKVKEAELELLKEKAKESRDEEKIK
                     QAEAEVESKQAEATRLENIKTDRKKAEEEAKRKADAKLKEANVATSDQGKPKGRAKRG
                     VPGELATPDKKENDAKSSDSSVGEETLPSSSLKSGKKVAEAEKKVEEAEKKAKDQKEE
                     DRRNYPTNTYKTLELEIAESDVKVKEAELELVKEEAKEPRDEEKIKQAKAEVESKQAE
                     ATRLEKIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPKPEKPAPAP
                     KPENPAEQPKAEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNG
                     AMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNA
                     NGDMATGWLQNNGSWYYLNANGDMATGWLQNNGSWYYLNSNGAMVTGWLQNNGSWYYL
                     NANGDMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDGYGV
                     NANGEWVN"
     gene            complement(52473..53453)
                     /gene="dusB"
                     /locus_tag="VW325_000408"
     CDS             complement(52473..53453)
                     /gene="dusB"
                     /locus_tag="VW325_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9733872.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(53440..54312)
                     /gene="hslO"
                     /locus_tag="VW325_000409"
     CDS             complement(53440..54312)
                     /gene="hslO"
                     /locus_tag="VW325_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9733873.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            54477..55904
                     /locus_tag="VW325_000410"
     CDS             54477..55904
                     /locus_tag="VW325_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9733874.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            56063..57571
                     /gene="glpK"
                     /locus_tag="VW325_000411"
     CDS             56063..57571
                     /gene="glpK"
                     /locus_tag="VW325_000411"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9733875.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            57613..59439
                     /gene="glpO"
                     /locus_tag="VW325_000412"
     CDS             57613..59439
                     /gene="glpO"
                     /locus_tag="VW325_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9733876.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESNWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVKAALAQNDLAELKN"
     gene            59507..60211
                     /locus_tag="VW325_000413"
     CDS             59507..60211
                     /locus_tag="VW325_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9733877.1"
                     /translation="MMSELFGEFLGTLILILLGNGVVAGVVLPKTKSNSAGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            60508..60711
                     /locus_tag="VW325_000414"
     CDS             60508..60711
                     /locus_tag="VW325_000414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733878.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(60798..61406)
                     /locus_tag="VW325_000415"
     CDS             complement(60798..61406)
                     /locus_tag="VW325_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733879.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT        19313 a        10396 c        13177 g        18926 t
ORIGIN      
        1 taatagagtc tgtgaaatct gtggaaaaca gcctttaaac aagtcatatc aagtttttta
       61 gcttgtttaa aaaatgtgga taaaaaagat aaaaaactaa aagttatcca caaattattt
      121 gattttcttt ttgattgatt caatttctaa acgtaaatta tcgtcttgat caatcaaaga
      181 ttttattttg gcatgggcat gaatgactgt ggtatgatct tttcccccaa attccttccc
      241 aatttttgga agactattat ctgttagttc tctagataaa tacatggcta cttgacgggc
      301 caaaacaata ttttgaaggc gtctacttcc cttcatttct ttgatactaa caccataaaa
      361 gttaccaact tcagtttgga ttttatcaat tgggatgacg agcatttggc taacatcttg
      421 tttgcgggct ctaatggctt ctgcagcaat atcaatagtg atatccttga ttttttttac
      481 tctggcaatt aaagtgatgt cgttgatggc tccctcaaga tctcgaacat ttgaatcaaa
      541 ttgcccagct aggtattcta gagtatcact ttggaaattg tagcctaaat gttccgtttt
      601 actttgtaaa atggcaatac gtgtttcaaa gtcagggggt gtgatagttt gtgtcaatcc
      661 ccaactaaaa cgcgtgacaa gcctctcctc gagcccttct agatgttttg gactacgatc
      721 actcgttagg acaatctgtt tttgcttgtc atgaagggcg ttaaaggtat tgaaaaattc
      781 ttcctgagtt gcgacttttt ttccgctgag tgactgtata tcatcgatta acaaaagatc
      841 aagactacga taggtctttt taaacttttc catttcccca agtcttaggt gatcaagaaa
      901 gtcattaata aagctttcgg cagggatata tttaacacgc gcattaggaa tattttttag
      961 aatttcattt ccaatagcgt ttaataagtg agtcttacca aggcctggtc ctccatagat
     1021 aaaaagaggg ttataggtca gagccaaatc ttcagagaca gctaaagcgg ctgatacagc
     1081 ccaaacattt ccatcccctt gaataaagtt atcaaaggta tacttttctt ttaatcccgt
     1141 atctgaataa ggaatagata ctaactttgg actatagtca taaagagtta aatttgtagc
     1201 ttcttcaact tgtgagctag tcgtatcttg aggtttggtg aaaatatagt ggggagttat
     1261 ttcagcgtca taaatttcaa aaccagctac tacaataata tcttttagtt gtttttccca
     1321 gaccatttcc atttcagagc gaggtagaaa tatagtggca acattttcct ctaccttgat
     1381 gagttcagct tgaatagcat agaaatcata catggatcga gtcagtcttt cttgtgcaaa
     1441 ttctaatata cgattccaaa attgtttttc tttcaatcct ttctcctcct ttactattta
     1501 aaagtttaat actagagata ttttaccata gatagcaaga attttccaca agctgtgaaa
     1561 aataatccac aatgttacta aactttatcc acaggttggg gataaaagaa gaaattattg
     1621 atttattaag ctttttatcg aaaaacatag tggataacct tgtgagataa aaaaataaaa
     1681 gaacagcctt atttcaggct gttgataatt ctactatatt cttcttgatt tgaaaaagaa
     1741 ataatgattt ttccactgtc ttttttagat agtttaattt ctacatctaa tccgagtagt
     1801 tttcttaact gtttttcttc attttgtatg aaatgattag ttttttgctg tttcttttgt
     1861 tttttctctg tcagaagagc ttctaacttc cttacagaaa tatcttcttc tataatccgt
     1921 tgaaagaaat agtcttgttg ttccttattt aacccaacta gggaacgcgc atgggcttgt
     1981 gatagtttgc cattttctac ttctgagaga atctgttctg gcaaggaaag taaacgaatg
     2041 gagttgctga tatatggacg agacttgccc atcttatctg caatttcagc atgggtgaat
     2101 cctttctcta cgagagattc ataggcgcgt gcttcttcta ttgggtttaa attttctctc
     2161 tgtaaatttt caataatgga ctggaccatc atctcttggt ctgaaatctg tttaacaaca
     2221 gctgggatag accgtagacc agctaaaagt gaagcccgat agcgtctctc tcctgcaagg
     2281 atttcataac caataacagg agattgacga acaataatcg gttgaatgac cccattttct
     2341 ttgatagact gtgctagttc atctagtttt tctctatcaa attcttttcg gggttgatag
     2401 ggattttttt gtatatctgt gatagaaatc atttcaaatt tttccatgat tctacactaa
     2461 cacatctttt ctcttatgta aagctttctt tacatagatg tcaattaaga ttctaaatca
     2521 cctgaactct tgttaagttt gatagaggta gtttcttctt tcccgttacg atagtaggtt
     2581 atcttaatgg tgtctccgat agaatggttg taaagagcac tttgtaaatc tgttgatgaa
     2641 gcaatctctt tgtcatctac ttttgtaatt acatcgtatt tttcaaggtg accattggca
     2701 ggcatattac tttgtaccga acgaacaact acaccagatg taacattact tggaatattg
     2761 agtcttctga tgtcgcttgt actcacatta gataaattaa ccatctggat tcccaaagct
     2821 ggacgagtca cttttccgtt tttttctaac tgttcaataa tattgatagc atcatttgca
     2881 ggaattgcga aaccaagacc ttctacagat gttcctccat ttgtagcaat tttacttgag
     2941 gtaattccga taacctgccc ttgaatattg atcagtgggc cgccagagtt acctgggtta
     3001 atagcagtat cagtttggat ggcttttgta gaaatagctt gtccatcttc cgatcttaag
     3061 gatacatttc tattgagact ggatacgata ccttgagtga cagtatttgc atattcagaa
     3121 cctaacgggc taccgatggc aatagcagtt tctcctacag ttaacttact agaatcacca
     3181 aactcagcta ctgttgtcac tttttctgaa gagattttga cgacagcaat atcagagaaa
     3241 gtgtcagctc cgacaatttc tccaggtact ttagtcccat ctgacaatcg aatatctact
     3301 ttgctggcgc catttataac gtgattgttg gtgacgatgt aagcttcttt atcattcttt
     3361 ttataaataa ctccagatcc ttcactagag attcgctgag aatctgtgtc agtatcatca
     3421 ttgccaaata cgctattttg tctgtttgcc gaataagtaa taacagaaac aacagcatct
     3481 tttactttgt taacagcctg tgttgttgaa ttttcgttct tataggcagt ttgtgtaata
     3541 gtactattgt tgttagagtt gtttacacta cttttttgag ttagttgagt tattgaaaaa
     3601 ctacccaagg ctccactaaa aaagctaatg acgataacga ctaataattg aaaccatttt
     3661 ttgtaaaatg tttttagatg tttcatattt gcctccatat gtttgaatta ctgaaagtat
     3721 aaactgacta gcttaattat aacttaaaca caaaagtttt acacaaactg tggataactc
     3781 ttttgaaact gtgattttct taattgaaat ctatttttta ttttgtgaat aagatgtgaa
     3841 aaaatagaga atatgttaga atagagtcat gaaaattaaa gttgtaacag ttgggaaact
     3901 gaaagaaaag tatttaaaag atggtatcgc agagtattca aaacgaattt ctagatttgc
     3961 taagtttgaa atgattgagt tatcagatga aaaaacacca gataaggcca gtgaatcaga
     4021 aaatcaaaag attttagaaa tagaaggtca gagaatttta tcaaaaattg ctgaccgtga
     4081 tttcgttatt gtgttagcca ttgaagggaa aactttcttc tcagaagaat ttagtaagca
     4141 gttagaagaa acttctataa aaggattttc tactcttact tttattattg ggggaagttt
     4201 aggattgtca tcatctgtaa aaaatagagc caatctttct gtcagttttg gtcgcctaac
     4261 cttacctcat cagttaatga gactagttct tgttgaacaa atctatcgcg cttttacgat
     4321 tcagcaggga ttcccctacc ataaatagaa aattgacttt taattgaatt tttggtagaa
     4381 taattgtgtt aggtctcata gctcagctgg atagagcatt cgccttctaa gcgaacggtc
     4441 gcaggttcga atcctgctgg gatcaatata atagcaaagc tgggaatttt cccggctttt
     4501 ttcttaaaaa agtacacttt gggagaaaaa aatgacagtt gagagaattt tatctaaaac
     4561 gaaattccat tttgtataat ggtttttgta agttagctta caagaaaaaa acattttagg
     4621 agattttatt atgaaaaaca cagttaaatt ggaacagttt gtagctttga aggaaaaaga
     4681 cttacaaaag attaaaggtg gggagatgag gctgtcaaaa ttcttccgtg attttatttt
     4741 acaaagaaaa aagtaatgaa ataaggggaa agagtaatgg atttatttgg atttgggaca
     4801 gttattgttc atcttttaat tattagtcac aactaccatt taatttgtaa aggtcaaata
     4861 aatagaaaag aattattcgt ttttggtgct tatacattac taacagaaat agtatttgat
     4921 tttcccttat atattctata tttagatggg ttagggattg caatattttt atttcctttg
     4981 ggcttatatt cctattttcg atggatgaaa cagtatgaga gggatagagg actattccta
     5041 agtttactac tatctctttt atatgagagc actcataact ttctgtccgt aactttctcc
     5101 tctataacag gagataattt tgttttacaa tatcatttcc cattcttttt cgttgtgacg
     5161 gtgttaacct attttgttac attaaaaatc atttattatt tccatttgga actagcctat
     5221 tttgacaagg actaccttta tcctttcttg aaaaaagtat tttttgcttt actattgcta
     5281 cctattgtat ctttcgtttc agatatggta agtacgatta aacatttgaa tagttttgga
     5341 agtattttgt catctattgt ctttatctct ctccttttga ccttctttgc aatgaattct
     5401 cataaagttc aaatggagaa agagattgct ttgaagcaga agaaatttga acagaaacat
     5461 ttacagaatt acacagatga aattgtcggt ctgtataatg aaatccgtgg ttttcgacat
     5521 gattatgctg ggatgcttgt cagcatgcag atggcaattg acagtggtaa tttacaggaa
     5581 attgacagaa tttacaatga agttttagtc aaagcaaatc ataaattgcg ttcagataag
     5641 tacacttact ttgatttgaa caacatagaa gactcagctt tacgaagttt ggttgctcag
     5701 tcaattgtct atgctcgaaa taatggtgta gagtttacac tggaagtaaa agatacgatt
     5761 accaagcttc caattgaact attggatttg gttcgtatca tgagcgtttt attgaataat
     5821 gctgtcgaag gatcggctga tagctataaa aagcagatgg aagtagcagt tattaagatg
     5881 gaaactgaaa cagttattgt gattcagaat tcatgtaaaa tgacgatgac tccttcagga
     5941 gatctatttt ccttaggatt ctccactaag ggaagaaatc gcggagtcgg attaaataat
     6001 gtgaaagaac tactagataa gtacaacaat attattttag aaacagagat ggaaggcagt
     6061 acatttagac aaatcattag atttaagagg gaatttgaat gaaagtttta attttagaag
     6121 atgttattga acatcaagtg agactagaga gaatattgga tgaaatttcg aaagaatcga
     6181 atattccaat atcatacaag acaacgggaa aagtccgtga atttgaagaa tacattgaaa
     6241 atgatgaagt aaatcagctt tatttcctag atatcgatat tcatggaatt gagaaaaagg
     6301 gatttgaagt ggctcagttc attcgtcatt acaatcctta cgctattatc gtctttatca
     6361 ctagtcgatc agagtttgcg actctaacct ataaatacca ggtatcagcc ctagattttg
     6421 ttgataagga tatcaatgat gagatgttta agaagagaat tgagcaaaat atcttctaca
     6481 cgaagagtat gttacttgaa aatgaagatg ttgtagatta tttcgactac aattacaagg
     6541 gaaatgattt aaaaattcct taccatgata ttttgtatat tgaaacaaca ggggtatctc
     6601 ataaattgcg cattattggt aagaattttg caaaagagtt ttatggtacc atgacagata
     6661 ttcaggaaaa ggacaaacat actcagcgat tttattctcc tcacaagtca tttttggtaa
     6721 atataggcaa tatcagagaa attgatcgaa aaaacttaga aattgttttc tatgaagacc
     6781 atcgttgtcc tatttcaaga ttaaaaatta gaaaattaaa agatatttta gagaaaaaat
     6841 ctcaaaagtg attgacaatt agcaagaaat tgatataatg gttatatctg ctacagatag
     6901 aaggtccgtt ggtcaagggg ttaagacacc gccttttcac ggcggtaaca cgggttcgaa
     6961 tcccgtacgg actattttat ccgccatagc tcagttggta gtagcgcatg actgttaatc
     7021 atgatgtcgt aggttcgagt cctactggcg gagtatagat aaaagggaac acagctgtgt
     7081 tcctcttttt gtatcaattt gtatcaccaa gcattttcat aaggaagtct gttatttctt
     7141 gagaactttc tttttttcca tgtgcaatcc aagtttggca gacaccaaaa agtgcatgag
     7201 ttagatagat gctactatat tctaattcag tggtatttag attcagttgc ataaatcgct
     7261 tttgtaaatc tgtactgagc atgatatgaa gtttatttcg taagaaattt tggatttctt
     7321 tagtcccatt ttcagaaaga agggcagcca gaagtggttc tgactctaga tattcaaaaa
     7381 cttctaaaat agcgtctctt ttgtgatgag catgtttttg aaaaatatat tcaaatgtat
     7441 ggaatagctt gctttgatag tgctcaatca tatcatactt atccttatag tgagtataga
     7501 agctggaacg actaattccg gctttttcta ctaatttgac agtagaaatt ttatcaaatg
     7561 gctgttccat cagtaattgt accatagcgt tttcaatagt tcgctttgtt tttaagcgtt
     7621 tgttactttc ttgcatattt cctccttgta aacaaattag actatatgtc taaaaataga
     7681 ttttttatct tgtaatttag attttttaat gtataatcta ttatatcaaa attttagaca
     7741 atatgtttaa aaaaggagaa actatgttta aagaatggaa agcaatttaa aaaaaaacaa
     7801 cctttattat tgtcatgatc gggatttctc ttattccagc tctgtacaat atcatatttt
     7861 tgtcatcaat gtgggagcca tatgggcaat tgtctgactt acctgtggca gttgtaaata
     7921 atgataaaga ggcttcctat aatggtaata ctatggcaat aggaaaagac atggtgtcca
     7981 atttaaaaga aaataaaacc ttggattttc attttgtaga tgaagaggaa ggaaagaagg
     8041 gattggaaga tggcgattac tatatggtag tgactttacc aagtgattta tctgaaaaaa
     8101 caactacatt atccaatatt caatcgacag cagcttatca atcattgaca agtgagcaac
     8161 aaactgagat aagtgattct gtatctcaaa attcaactga tagtattcaa tcggctcagt
     8221 caattgtagc tttagtacaa ggtttacagg gaagtttaga aaacttacaa aatcaatctt
     8281 ctaatctttc gactttaaaa aatcaagcta atcaagtatt acctattact tctacttctt
     8341 tgataggatt gtcaagtgga ttaacagaga tacaaggaga tgttactagc aaattagttc
     8401 ctgccagtca gtcgattgca tcaggtgtaa acgcatatac tacaggtgtt gataaagttt
     8461 ctcagggcgc aagtcaacta agtgaaaaaa atgccacctt gacaggtagt ttggataaac
     8521 tagtttcagg ctcaaacacc ttgacacaaa aatcttctag attgacagca ggagttggtt
     8581 aattacaatc aggatctggg caattagcag acaaatccag tcagttactt tcaggtgctt
     8641 ctccattaga gaatagagct aataaattgg cagatggatc taggaaacta gcagaaggtg
     8701 gaacaaagtt aacttctgga ttggaagatt tacagacagg acttgcttct ttaggacaag
     8761 gactaggtaa tgctagtgat caactcaaat cagtatcaac agaatctaaa aatgcagaga
     8821 ttttgtcaaa tccactcaat ctttcaaaaa cagacaatga tcaagttcct gtaaatggaa
     8881 tcgcaatagc tccttatatg atatcagttg ctcttttttt tgcagcaata tcaacaaata
     8941 tgatatttgc gaaattgcct tcaggacgtc atccagagag ccgttgggct tggttgaaat
     9001 cttgagctga aataaatggt attatagctg ttttggcagg aattttggta tatggaggag
     9061 ttcagcttat tggtttaact gctaatcatg agatgagaat atttattctc atcatcctaa
     9121 caagtttagt attcatgtct atggtgacca ctttagcaac gtggaatagc cgtataggag
     9181 cttttttctc acttattttg cttttactac agttagcatc aagtgcaggt acttatccac
     9241 ttgctttgac aaatgatttc tttagatcta ttaatccctg gttaccaatg agctattcag
     9301 tttcgggatt acgacaaaca atctctatca acaagtcatt ttcctagctg tcatactagc
     9361 tctatttact ggtttaggta tgctagccta tcaacataag aaaatggaag aagattaaaa
     9421 aaatcgaccg attaactggt cgatttttta tgccttagat gactttcgtc tgtgattata
     9481 gattccaaat agtaagagag aagtaaagga acagattgct ccagtaataa aaccattggg
     9541 aatgaaggaa agtgtaatag ttcctttccc cttgggaatg tcaactttca taaatccagt
     9601 ttgagcttgt ttaatttcta ttttcttacc atcttggtag gcagaccaac ctttgtcata
     9661 aggaatggtg aagaaaatag atgtatcttg ttggacatca tatgtagcaa aaaccttgtt
     9721 tttagaagtt gatactgtga caggttgttc tttaattttt tgaattgcct cggtgaaagt
     9781 tttggtatct aaacgataga aggtaggaga ttcaaatgat acttgtgaat ttccagggaa
     9841 actaacattg atattgaaag tttttttctc tttagtatat cctagattaa agaaggagaa
     9901 gacattatca gttgtaaaag tctttttttc accatttaca aggatgtcaa ccttcttttg
     9961 tttatcgtta gaaaagtgaa ggtttatgaa agagagataa acttggctgt tttctggaac
    10021 ttcaatttga tactggattg ctgcatcttc atttgaagaa cttgtgacac taatcaaatc
    10081 attagtattt tctatttttt ctgttttttc ataaggtatt ggagaaaaat aatcaaaatt
    10141 gacgttagca agttgattta aaaatgaggc ctgattatcc aaggtatgtt cattgaactt
    10201 gacatcattg taaacagatt gactagcaac tgcaatcgga agagagtatt gattttcata
    10261 tagggtaaga ttatcttttt gatagatatc tttaaagcca tacttatcaa taggactgtc
    10321 tgagatattg tactggatac caaataaact atcagccaaa atactattat ttgcatatcg
    10381 gagattgaga ttagtcccag aggatttaaa accaagttta tctaaagtag agcttgatga
    10441 acgatttcga acagatgaaa attgagagat tccattgtag ttgaatttca tactgtcatt
    10501 tcctgtctga gtttgtagtt tttcagtacg agtaaattga tttccaatat atgttgagaa
    10561 agattccata gctgggatat ctcgactata agcacttcga gaagcaaatc cccattcctt
    10621 agcaattccg tccatttgag atgaagcatt taaactcatt tcaaccagta taaataaaga
    10681 gattagaatg gcaaatagat tcacagatat aaactttttg ataactgcaa ggagtaaaag
    10741 agaatagaca accaaaaatt caagagtaag cagaatattc aaatctgtta aaaaagaata
    10801 atgcgatttt agatagatgg tagctaaaaa tcctgctact acaagaaaaa gcgaaactaa
    10861 aaaattccag actttaagtt ctttcagacg atttaagact tctgctgctg tgtaaattaa
    10921 caaggtagag aaaatccaag catagcgatg taaaaacatg tttggagtat gcatgccttg
    10981 ccaaaataag tcaagagctt ctatgtaaaa gcttgcaatt agaaatgcaa agaatattac
    11041 atatatgagt ttcacgtgaa acttaataga tttcagcgta aaaaataaaa tggtcaaaat
    11101 aaagggaagt agtccaacaa aaatcattgg gatggcccca tactttgttg tgtcaaagga
    11161 accaatgaat tgcttagcaa agagatcaag ataccagcta ctttcagttt gaaactttgt
    11221 aacttcagtc aatttttccc catgtgtctg taaatcaaac agagtgggaa gagtcataat
    11281 caaactagcc ataccagcta aaaaggagat aactatgaaa tcaagaacag atgattttcg
    11341 agtcttaaag tcccacgaaa tttgacagag ataccagaaa ataagaaaca atactgtcat
    11401 atatccaaaa taataatttt gaataaataa gattgacaga cttgtaaagt acaataggag
    11461 tttcttttca gttatcagta gatgtaaacc agttataatt aaaggaatca agataaaaac
    11521 atctagccag gtttttatct ctaattgact gacagtgaaa ctcatcagag cataggaagt
    11581 agataaggct agttttaaaa tctgagggat agattgaaac aatttattca aactaaaaaa
    11641 ggttgacaga ccaatcaatc caaattttaa gagagttgtc agatagatag catctggcat
    11701 attcgttaga tcaaaaaagt aaaccagagg cgcgagaaaa ctacccaagt aataactaga
    11761 tagggcatag aagtttagcc ctagaccact tgtaaaggtg taaaacagat tactatttcc
    11821 atgtaggata tttcgtaagg ctacatcaaa aataacgtat tgatgaaagc catctcctaa
    11881 tagaggagag ttgtcgctat tccagtagat accttgagat agatatactc cagacataat
    11941 cactacagga atgatgaaag aaataaaata ggttcgatat gtttttaaaa atgatttcat
    12001 gttacctcgt agaatgatag aaaactcagt tggttaaccc aactgagttt tgaagtttta
    12061 tttagtcttt ccaaagttct ttaacttttg cttgtatttc tgcattttct aggaattcat
    12121 cgtaggtttc atcgatacgg tcaatgacgc catttttaga taagacaatg atatggttag
    12181 ccaaagtttg aataaattcg tggtcatggc tggcaaagat gattgattct ttaaagtttt
    12241 tcaatccatc attcaagctt gagatagatt ccaagtccaa gtgatttgtt ggatcatcaa
    12301 gtacaaggac atttgatttt aagagcatga gttttgaaag catgacacga actttttctc
    12361 cccctgacaa gacatttaca ggtttgttaa cttcatctcc agagaagagc atacggccga
    12421 ggaagccacg taggaaagta ttgtcatctt cttctttact tgcgaattga cgcaaccagt
    12481 caagaattga ttctcctcct gcaaaatcag ctgagttatc ttttggtagg taagattgac
    12541 tagttgtaac tccccacttg acagttcctt catagtcaat atctcccatg attgcacgaa
    12601 ttaatgcagt cgtttgaata tcattttgtc caataagtgc tgtcttatca tctggacgca
    12661 agatgaaact aatattatcc aagatagttt caccatcaat ctttacagtt aaattttcta
    12721 ctgtcaagag atcattacca atctcacgtt ccgctttaaa gttgataaat ggatatttac
    12781 gactagatgg cacaatctct tctagctcaa tcttatcaag cattttctta cgtgatgttg
    12841 cctgccttga cttagaagca ttggcagaga aacgagcaac aaattcttgc aattgtttaa
    12901 ttttttcttc tgctttagca ttacggtctg ctagcaattt agcagcaagc tcagaagatt
    12961 ccttccagaa gtcgtagttt ccgacataga gtttgatttt tccaaagtca aggtcggcca
    13021 tgtgagtaca aactttgttt aagaagtgac ggtcgtggga tactacgata actgtgttat
    13081 caaagtcaat caagaagtct tctaaccaag taatcgattg gatatccaaa ccgttagtag
    13141 gctcgtccaa gagaagaaca tctggtttac caaaaagtgc tttggcgagg agaaccttta
    13201 ctttttcacc gttggccaat tcgctcatgt tttggtagtg taattcttct ggaatgttta
    13261 ggttttgaag tagttgagag gcttcactct ctgcttccca acctccaagt tcggcaaact
    13321 ctccttcgag ttcggcagca cgaaccccgt cctcgtctga gaaatcttcc ttcatgtaga
    13381 tagcatcttt ctctttcatg atgctataaa gtttttcatt tcccatgata acgacatcaa
    13441 tggcacgttc atcttcgtag tcaaagtgat tttgacgaag aacagagaga cgttcatctg
    13501 gaccaagaga gatgtgacca gtagtaggtt cgatatctcc agctaaaatt tttaaaaagg
    13561 ttgattttcc ggcaccatta gcaccgatta atccgtaagt atttccttct gtaaatttga
    13621 tattgacatc atcaaaaagt ttgcgatcac taaaacgtag tgaaacatca gatactgtaa
    13681 gcaatgtttt tctcctatat gtgtaatata tttattctac tagaaaatac agaaatattc
    13741 aaatttttat ttgtcaattt tgtgtaaatt atatttacag tatcctttac acaaatctgt
    13801 aaaaagcaag gctgatttat tttgataaat tacggttatt tcattaaaaa aatgctataa
    13861 ttgaaaggac tatatcgaag gagaacaaaa tgactaaacc cattatttta acaggagacc
    13921 gtccaacagg aaaattgcat attggacatt atgttggaag tctcaaaaat cgagtattat
    13981 tacaggaaga ggataagtat gatatgtttg tgttcttggc tgaccaacaa gccttgacag
    14041 atcatgccaa agatcctcaa accattgtag agtctatcgg aaatgtggct ttggattatc
    14101 ttgcagttgg attggatcca aataagtcaa ctatttttat tcaaagccag attccagagt
    14161 tggctgagtt gtctatgtat tatatgaatc tagtttcgtt agcacgtttg gagcgaaatc
    14221 caacagtcaa gacagagatt tctcagaaag gatttggaga aagcattccg acaggattct
    14281 tggtctatcc aatcgctcaa gcagctgata tcacagcttt caaggctaat tatgttcctg
    14341 ttgggacaga tcagaaacca atgattgagc aaactcgtga aattgttcgt tcttttaaca
    14401 atgcatataa ctgtgatgtc ttggtagagc cggaaggtat ttatccagaa aatgagagag
    14461 cagggcgttt gcctggttta gatggaaatg ctaaaatgtc taaatcacta aataatggta
    14521 tttatttagc tgatgatgcg gatactttgc gtaaaaaagt aatgagtatg tatacagatc
    14581 cagatcatat ccgcgttgag gatccaggta agattgaggg aaatatggtt ttccattatc
    14641 tagatgtttt tggtcgtcca gaagatgctc aagaaattgc tgatatgaaa gaacgttatc
    14701 aacgaggtgg tcttggtgat gtgaagacca agcgttatct acttgaaata ttagaacgtg
    14761 aactgggtcc tattcgtgag cgccgtattg aatttgctaa ggatatggga gaagtttata
    14821 atatgattca aaaaggtagt gaaagagcgc gtgaagttgc gggtcaaacc ctatctgagg
    14881 taaaaggggc aatgggactt cattacttta actaagttta ttttacaaga aactatcttt
    14941 tctatctcga agaaaagata gttttttatt tacccctgta acatttgaca aatttttata
    15001 gaatggtatg atagatacaa tataaaaaga gtcaagctca aaaagaaaga aaagaggaaa
    15061 cttcgaatgt ctaattggga cactaaattt ttgaaaaaag gttttacctt tgatgatgta
    15121 ttgcttattc cagctgaaag tcatgtgttg cctaacgatg cagatttaac aactaaattg
    15181 gcagataatt tgactttaaa tatcccaatt attaccgctg ccatggacac agttacagag
    15241 agtcaaatgg ccattgctat tgctcgtgca ggcggtctcg gagttatcca taaaaacatg
    15301 tcaattgctc aacaagcaga cgaggttcgt aaggtaaaac gttctgaaaa tggagttatt
    15361 attgatccgt tcttcttgac gcctgaacat acaattgctg aagcagatga gcttatgggt
    15421 cgttaccgca tcagtggtgt tccagttgtt gaaacacttg aaaatcgtaa attggttggt
    15481 attttgacaa accgagatct tcgttttatt tcagattata atcaaccaat ttcaaaccat
    15541 atgactagtg aaaatcttgt tactgctcct gtgggtacgg atcttgcaac ggctgagagt
    15601 attcttcaag agcatcgtat tgaaaaactt ccgttggtcg atgaagaagg cagtctttct
    15661 ggtttgatca ctatcaaaga tattgaaaaa gttattgagt ttccaaatgc agctaaagat
    15721 gagtttggtc gtcttctagt tgcaggtgca gtaggtgtta cttcagatac atttgaacgt
    15781 gcagaggctc tttttgaggc aggagcggat gcgattgtta ttgatactgc acatggtcat
    15841 tctgcaggtg tcttgcgtaa aattgccgag attcgtgctc atttcccaga tcggactttg
    15901 attgctggaa atattgctac tgctgaaggt gcacgtgccc tttatgaagc gggtgtagac
    15961 gttgttaagg ttggtattgg accaggttct atctgtacta ctcgtgtgat tgctggtgtt
    16021 ggtgttccgc aagtaacagc tatctacgat gctgcagctg ttgcgcgcga atatggtaaa
    16081 acgattattg ctgacggtgg gatcaagtat tctggagata ttgtaaaagc acttgctgca
    16141 ggtggaaatg ctgttatgct tggatctatg tttgctggaa ctgatgaagc tccaggcgaa
    16201 actgaaatct tccaaggacg taaattcaag acttaccgtg gtatgggatc aattgctgct
    16261 atgaagaaag gttcaagcga ccgttatttc caaggttctg tcaatgaagc aaacaagctt
    16321 gttccagaag gaattgaagg tcgtgttgct tataaaggag cggcagctga tattgttttc
    16381 caaatgattg gtggtattcg ctctggtatg ggttactgtg gtgcagctaa ccttaaagaa
    16441 ctacacgata atgctcaatt tattgaaatg tctggtgctg gtttgaaaga aagccatcct
    16501 catgatgtgc aaattactaa tgaggcacca aattattcta tgtaaaaaac aatgaaaaga
    16561 actccagtga aaacaggagt tcttttacaa tgttgtcaat ttccatttac agcagcttta
    16621 ccatcctgaa tagtgaagat acttagattt tctggcagat tttgaagatg gtctaagctt
    16681 gttgttgtga taaaggtttg gattgattga gaaatcgttt ctaataattt taactgtcta
    16741 gtgttgtcaa gttcactcat cacatcgtca agcagtaata taggagattc tgtggtaatg
    16801 ctttccatta attcgatttc tgctaatttt atcgagagga cgagactacg atgttgacct
    16861 tggcttccga aactagcatc catcccattt atataaaaag aaatgtcatc tcgatgagga
    16921 ccgacaccag tattcttttt aaataaatct ctggatctac ttttttctaa agcaattttg
    16981 aaagattcgg ataagttttg tttgtcagtt atattgacag aagattgata ggatattgac
    17041 aactcttcga tctgattaga gagttcaaaa tgtttcttac gcccaaatga ttctagtttt
    17101 tttatgaaat ctaagcggtg attcattaca cgacatccat aatcaactag ctgatcatct
    17161 aacacagaaa ggaatgtttc atctattttt tgagctgatt ttaggtaagt gtttctttgc
    17221 tttaggatgt ggttataatt ggttaagtca gataaataga ttggcttaat ttgcccaagt
    17281 tccatatcaa tgaattttcg tcgaatcgaa ggtgctcctt taattagttg taaatcttca
    17341 ggagcaaata agacaacatt catgtgtcct acataatctg aaaggcgtgc ctgttttaag
    17401 tgattaactt ttgttacacg acctttttgt gttagttcga tttctagggg aatggatcca
    17461 gtttttttct gaacgagacc tgaaagatga agttgttcct catcaaaatg aatgagattt
    17521 ttatctgttc gagttcgatg actacgcgtt aaggctaaaa aatagatagc ctctaacatg
    17581 tttgtttttc cttgtgcatt acgtcccaaa aagacattca atttaggatt aaagtctatt
    17641 ttcgtttctt tgtaattacg aaaagtcttg agagatagat gttgtagcca catgattatc
    17701 taccagggaa tcgtggagct tgttggcttt taggtgatga agcaggtttc gatttgtctt
    17761 ttttaactcc cttattcatc tttttgacaa gtttagcgat ccgttctttt tcaactttat
    17821 cagcttggta ttcatcttgt tcttcagaag taggttgtgt caacaagatg tcaatgttca
    17881 tgtcagggat gtcaacttta tcgccaatac gaagtttttt tccacgacgg ctctctaatt
    17941 ccctattaaa gtaaacagaa tgttcagaga gaaatgattt aatagctcct ccgctatgtg
    18001 taattccaag ttctttgagt agtgcttgga gggtaataaa ttcttcaaat aatttgtatt
    18061 ccataattca cctcaatctt tggtattata ccatattttc ctaaaaatag tgagagagac
    18121 agcgagaaat gtaagcgatt tttatttcaa aagtgttaca gggaacaaga aaatttcagg
    18181 ttttcgtgat ataatagaag tctgtatata aggaggtaaa tcatggagtt agtgcatgga
    18241 atttcaacac attttatcca atcaaaaaag tttaaaacaa acaaaattac cgtgcgtttt
    18301 accgctccat tatcccttga tacgattgca ggtcacatgt tgagtgcaag tatgctagag
    18361 actgctaatc agatgtaccc cacttctcaa gatttgagga gacacttggc cagtctatac
    18421 ggtacagata tgtcaaccaa ttgtttcaga agagggcaaa gccacattat agaattgaca
    18481 tttacctatg ttcgtgatga gtttttaagt aggaaaaacg tgctaacctc tcagattttg
    18541 gaacttgtaa aagaaactct tttttcaccc gtagtagttg ataatgggtt tgatccggcc
    18601 ttatttgaaa ttgagaaaaa acaattgcta gcaagtttag cagctgatat ggatgattct
    18661 ttttattttg cacataaaga attggataaa ttgttttttc atgatgaacg tcttcaattg
    18721 gaatatagtg atttacgaaa tcgtatttta gctgaaactc cacaaagttc ttattcttgt
    18781 ttccaagaat ttttagccaa tgatcgaata gatttctttt tcctaggtga ttttaatgag
    18841 gttgaaattc aaaatgtatt agaatcattt ggctttaaag gtcgaaaagg agatgtgaag
    18901 gttcagtatt gtcaacctta ttctaatatc cttcaggaag gtatggttcg gaaaaatgtg
    18961 ggacaatcca ttttggaatt aggttatcat taccgttcta aatatggtga tgaacaacat
    19021 ttacccatga ttgtaatgaa tggtttactt ggtggatttg ctcactctaa gctctttaca
    19081 aatgtccgtg aaaatgctgg attagcttat accatttcaa gtgagcttga tttatttagt
    19141 ggattcttga ggatgtatgc tggtatcaat cgagaaaatc gtaaccaggc tcgtaaaatg
    19201 atgaataatc aactgcttga tttaaaaaaa ggttatttta cagagtttga gttaaatcag
    19261 accaaggaaa tgattcgttg gtcgttgtta ctttctcaag ataatcaatc ttcattgatt
    19321 gaacgtgctt atcaaaatgc cttatttgga aaatcttcag cagactttaa aagttggatt
    19381 gcaaagcttg aacaaattga caaagatgct atttgtagag tagctaataa tgtgaaacta
    19441 caagcgattt actttatgga aggaatagaa tgacaaaggt tgtttttgaa gaaaaatact
    19501 atccagctgt aaaagaaatg gtttatcgaa ctcgtttggc caacggattg acagttgctc
    19561 ttttgcctaa aaaggaattt aaagaggttt acgggagtgt cactgtacag tttggttcgg
    19621 tagatacgtt tgtcacagaa gttgacggag atgtaaaaca atatcctgga ggaattgctc
    19681 attttcttga acataaatta tttgagagag aagattctag tgatttgatg tcggctttta
    19741 cgagtctagg tgcagatagt aatgccttta caagctttac aaaaacaaac tatctttttt
    19801 cagcaacgga ttatttttta gaaaatttag atttacttga tgaattggta acatcagcac
    19861 actttactga agcttccatt ctgacagagc aggatattat tcagcaagaa cgagaaatgt
    19921 accaagatga tccagattcg tgtttattct tttcaacttt agcgaatttg tatcctggta
    19981 cacctttagc aactgatata gttggaagtg aggagtccat ttcccaaatc aatctaacta
    20041 atttgcaaga aaattttaca aagttttaca aacctgtaaa catgtctctg tttttagttg
    20101 gtaattttga tgtggagcga gtacaggact attttgaaag caaagaactg aaagattcag
    20161 attttcagga agtagcaaga gaaaagttgt ttttacagcc tgtaaagcca acagatagta
    20221 tgagaatgga agtatcttct cccaaactag cgattggagt tagaggtaag cgagaagttt
    20281 ctgaagcgga ttgctatcga catcatattt tattaaaatt attgtttgca atgatgtttg
    20341 gttggacttc ggatcgtttt caaaaatgtt atgaatcagg taaaattgat gcgtccttat
    20401 ctctggaagt tgaaataaca agtcgctttc attttgtcat gttgacaatg gatacgaaag
    20461 agccagttgc tttgtctcat caatttagga aggctattcg taattttaca aaggatttag
    20521 atattacaga ggaacattta gatattatca aaagagagat gtttggcgaa tttttcagta
    20581 gcatgaactc tcttgaattt attgcaacgc aatatgatgc ttttgaaaat ggtgagacaa
    20641 tttttgattt gccgaaaatt ttacaggaaa ttactttaga ggatgtcctt gatgctggac
    20701 atcatttaat agatgatggt gacatagttg attttacaat attcccatcg tagtaaccta
    20761 ttataataga cactagaaag aagggatgac aagtatgaga aaaaaaacaa ttggagaggt
    20821 tttacgatta gctagaatca atcagggatt gagtttagat gaattgcaga aaaagacaga
    20881 aatccagtta gatatgttgg aagcaatgga agcagacgat ttcgatcaac ttccaagtcc
    20941 tttttacacg cgttctttct tgaaaaaata tgcatgggct gttgagttag atgaccaaat
    21001 tgttttggat gcttatgatt ctgggagtat gattacttat gaggaagtag atgttgatga
    21061 agatgagttg acaggtcgta gacgttcaag taagaaaaag aagaaaaaaa tatcattttt
    21121 acctttattt tattttatcc tttttgcttt atcgatttta atttttgtga cttattatgt
    21181 ttggaactat attcaaactc aaccagagga gccttctctt tctaattaca gtgtggttca
    21241 atcaacaagt tcaactagct ctgttcccca ctcctcaagt agtagttctt ctagtataga
    21301 atcagctata agtgtatcag gcgaaggaaa tcatgtagaa atcgcttata agacaagtaa
    21361 ggaaacagtt aaattgcaat tggcagtttc agatgttaca agttgggtca gtgtttcaga
    21421 aagcgaactt gagggcggtg taaccttatc gccaaagaag aaaagtgcag aagcaacagt
    21481 tgcaactaaa agtcctgtaa caattacgtt aggtgttgta aaaggtgttg atttgacagt
    21541 agataatcag actgttgatt tatcgaaatt aacagctcag actggacaaa tcactgtaac
    21601 ctttactaaa aattaaggaa aaacgaatga aaaaagaaca aattcccaat ctcttaacaa
    21661 taggtcgaat tctctttata cctattttta tctttatttt aacgatagga aattcgatag
    21721 agagtcatat agttgcagct attatctttg ctgttgccag tattaccgac tatttagatg
    21781 gatatttagc tcgtaaatgg aatgtggtca gtaattttgg taaatttgca gatcctatgg
    21841 cggataagtt actagttatg tcggctttta ttatgttgat tgagttaggt atggctccgg
    21901 cttggattgt tgcagtgatt atctgtcgtg agttagctgt gacaggttta aggcttttat
    21961 tggttgaaac tggtggaaca attttagcag cagcaatgcc tggaaaaatt aaaactttta
    22021 gtcagatgtt tgccattatt ttcttgctat tacattggac tttgcttggt caagttctac
    22081 tttatgtagc cttatttttc actatctact ctggctatga ctatttcaag ggtagtgcct
    22141 atgtatttaa agggacattt ggttcgaaat gaaatcaata attgatgtaa aaaatctttc
    22201 ttttcgctat aaagaaaatc agaactacta cgatgtgaag gatattacgt ttcacgtgaa
    22261 acgtggagaa tggctttcga ttgtagggca taatggtagt ggtaaatcaa cgacggttcg
    22321 attaattgat ggcttactgg aagcagaatc cggagagatt gtaattgatg gccaacgttt
    22381 gactgaggaa aatgtttgga atatacgtcg tcaaatcggt atggtttttc aaaatccaga
    22441 caatcaattt gttggagcga ctgttgaaga tgatgttgct tttggtttgg aaaatcaggg
    22501 actttctcgt caagaaatga aaaagagagt ggaagaagct ctggctttag ttggcatgtt
    22561 ggactttaaa aagagagagc cagcgcgtct atcaggtggc caaaagcaac gtgtggccat
    22621 tgcaggtgtt gtagccctaa gaccagctat tttaatctta gatgaagcaa cgagtatgtt
    22681 ggatcctgag gggcgtagag aacttattgg gacagtaaaa ggaattcgaa aagactatga
    22741 tatgacggtc atttctatta cccatgattt ggaagaagtc gccatgagtg atcgcgtatt
    22801 ggtcatgaaa aaaggggaaa ttgaatcaac tagtagtcca agggagcttt tctctcgaaa
    22861 tgatttagat caaattggat tagacgatcc ttttgctaat caattaaaaa aatctttgag
    22921 ccagaatggc tatgatttac ctgaaaatta tttgacagaa agtgagctag aggataagct
    22981 atgggaattg ctctagaaaa tgtgaatttt acatatcaag aaggtactcc cttagcttca
    23041 gcagctttgt cggatgtttc tttgacgatt gaagatggct cttatacagc tttaattggg
    23101 cacacaggta gtggtaaatc aactatttta caactcttaa atggtttatt ggtgccaagt
    23161 caagggagtg tgagggtttt tgatacctta atcacctcga cttctaaaaa taaagatatt
    23221 cgtcaaatta gaaaacaggt tggcttggta tttcagtttg ctgaaaatca gatttttgaa
    23281 gaaacggttt tgaaggacgt tgcttttgga ccgcaaaatt ttggagtttc tgaagaagat
    23341 gctgtgaaga ctgcgcgtga gaaactggct ctggttggaa ttgatgaatc actttttgat
    23401 cgtagtccgt ttgagctgtc agggggacaa atgagacgtg ttgccattgc aggcatactt
    23461 gccatggagc cagctatatt agtcttagat gagccaacag ctggtctaga tcctctaggg
    23521 agaaaagagt tgatgaccct gttcaaaaaa ctccaccagt cagggatgac catcgtcttg
    23581 gtaacgcatt tgatggatga tgttgctgaa tatgcgaatc aagtctatgt aatggaaaag
    23641 ggacgtttag taaagggggg caaaccaagt gatgtctttc aagacgttgt ttttatggaa
    23701 gaagttcagt tgggagtacc taaaattacg gccttttgta aacgattggc tgatagaggc
    23761 gtgtcattta aacgattacc gattaagata gaggagttca aggagtcgct aaatggatag
    23821 tatgatttta gggcgttata tcccagggga ttcgattgtt caccgattgg atccacgtag
    23881 caaattgctt gctatgatgc tactgatttt gattgtgttt tgggctaata accccttgac
    23941 gaatctgatt ctttttatag cgacagggat atttattgcc ttgtcaggag tatctctttc
    24001 attttttatt cagggcttga agtctatgtt tttcttgatt gccttcacaa ctatttttca
    24061 actatttttc atttctaatg ggaatgtttt atttgagttt tcgtttgtga gaatcacgga
    24121 ttatgctttg caacaagctg ggattatttt ttgtcgcttt gtattgatta ttttcttttc
    24181 aactttgtta accttaacaa ccatgccttt aagtttggca tcagctgtcg aagctttatt
    24241 agcaccttta aagcgtgtga aagttccagt tcatgaaatt ggattgatgc tgtctatgag
    24301 tttgcgtttt gtcccaacct tgatggatga tacgactcgg attatgaatg cacagaaagc
    24361 tcgtggagtg gattttggag aaggaagcat cgttcaaaaa gtaaaggcga tgattcccat
    24421 tttgattcct ctttttgcga caagtttaaa acgtgcagat tccttggcta tcgctatgga
    24481 agcgcgtggc tatcagggtg gaaaaggcag aagtcaatac cgacaattga aatggactct
    24541 gaaggatacg ctgaccattc ttgttattct cgtacttggt tgttgtttat ttttcttaaa
    24601 atcttagtag ctttcaggaa ttgataaaaa gttactgtaa cagtttttga tataaaggta
    24661 ggaatatgaa ccgttttaaa aaatcaaaat atgtcattat tgtttttgtc actgttctgc
    24721 ttgtgtcagc tctcttagcg acgacttatt caagtacaat tgtgacaaaa ttaggagatg
    24781 gaatctcatt ggttgataga gttgtacaaa aaccttttca gtggtttgat tctgtcaaat
    24841 cagatttggc tcatttgaca cgaacatata atgaaaatga aagtttgaag aaacagcttt
    24901 accaattaga agttaaatca aatgaggtgg aaagtttaaa gacagaaaat gaacaactgc
    24961 gccaattgct tgatatgaag tctaaattgc aagccacaaa gactttagca gcagatgtta
    25021 ttatgcgttc tccggtatct tggaagcagg agttgacctt agatgcaggt agatcaaaag
    25081 gtgcttctga gaacatgtta gctattgcaa atggtggctt gattgggagt gtttcaaaag
    25141 tagaggagaa ctctactata gtcaaccttc tgacaaatac ggaaaatgct gataagattt
    25201 ctgttaaaat tcaacatggc tctactacaa tttatggaat tattattggc tatgacaagg
    25261 aaaatgacgt tcttaaaatt agccaattaa atagtaatag cgatattagt gcgggagata
    25321 aggtgactac tggtggatta ggaaacttta acgttgctga tattcctgtt ggtgaagtgg
    25381 ttgccacaac gcatagtaca gactatttga cacgagaagt aactgttaaa ttgagtgcag
    25441 atactcataa tgtagatgtg atagaattag tggggaattc ataatgagac agttgaagcg
    25501 agttggagta tttttattgc ttcctttctt tgttctaatt gacgcccata ttagccagct
    25561 tctgggctca tttttccccc atgtacattt ggctagtcat tttctttttc tatttctctt
    25621 atttgagacg atagaagtat cagagtatct ctacctagtc tattgttttg ttataggctt
    25681 ggtttatgat gtttactttt tccatctaat agggattaca actctcttat ttatcttatt
    25741 gggagccttc cttcataaat tgaatagtgt tattttgttg aatcgttgga caagaatgct
    25801 agctatgatt gtgctgacat tcctgtttga aatgggtagt tatcttttgg cttttatggt
    25861 agggttgaca gtagatagca tgtcgatttt tatagtctat agcttggtac cgacgatgat
    25921 tttaaatttt ttatggatta ctgtttttca atttattttt gaaaaatatt atctataaga
    25981 acgacatata aatgtaacaa aggcgtaata tttattaggc ctttttttgg tatactagta
    26041 ttgtctttaa aagaaggagt atctacgtaa tatgaagaaa aaaatcttag cgtcactttt
    26101 attaagtaca gtaatggttt ctcaagtagc tgttttaaca actgcgcatg cagaaacgac
    26161 tgatgacaaa attgctgctc aagataataa aattagtaac ttaacagcac aacaacaaga
    26221 agcccaaaaa caagttgacc aaattcagga gcaagtatca gctattcaag ctgagcagtc
    26281 taacttgcaa gctgaaaatg atagattaca agcagaatct aagaaactcg agggtgagat
    26341 tacagaactt tctaaaaaca ttgtttctcg taaccaatcg ttggaaaaac aagctcgtag
    26401 tgctcaaaca aatggagccg taactagcta tatcaatacc attgtaaact caaaatcaat
    26461 tacagaagct atttcacgtg ttgctgcaat gagtgaaatc gtatctgcaa acaacaaaat
    26521 gttagaacaa caaaaggcag ataaaaaagc tatttctgaa aaacaagtag caaataatga
    26581 tgctatcaat actgtaattg ctaatcaaca aaaattggct gatgatgctc aagcgttgac
    26641 tacgaaacag gcagaactaa aagctgctga attaagtctt gctgctgaga aagcgacagc
    26701 tgaaggggaa aaagcaagtc tattagagca aaaagcagca gctgaggcag aggctcgtgc
    26761 agctgcggta gcagaagcag cttataaaga aaaacgagct agccaacaac aatcagtact
    26821 tgcttcagca aacactaact taacagctca agtgcaagca gtatctgaat ctgcagcagc
    26881 acctgtccgt gcaaaagttc gtccaacata cagtacaaac gcttcaagtt atccaattgg
    26941 agaatgtaca tggggagtaa aaacattggc accttgggct ggagactact ggggtaatgg
    27001 agcacagtgg gctacaagtg cagcagcagc aggtttccgt acaggttcaa cacctcaagt
    27061 tggagcaatt gcatgttgga atgatggtgg atatggtcac gtagcggttg ttacagctgt
    27121 tgaatcaaca acacgtatcc aagtatcaga atcaaattat gcaggtaatc gtacaattgg
    27181 aaatcaccgt ggatggttca atccaacaac aacttctgaa ggttttgtta catatattta
    27241 tgcagattaa tttacagagg gactcgaata gagccctctt ttcaggtttt accgtgacaa
    27301 tccctattaa aaattatatc aaaatagctt gaaaatattg gaaaagtatg gtaaaatgaa
    27361 aattgtcgtg tgaacgataa tactcattct tgatgaattg tgaagcagtt gcccttgggt
    27421 cgttttgcga gttgaagtca agaagaggaa aaaaacaaaa aggagaaata ctcatggcag
    27481 taatttcaat gaaacaactt cttgaggctg gtgtacactt tggtcaccaa actcgtcgct
    27541 ggaatcctaa gatggctaag tacatcttta ctgaacgtaa cggaatccac gttatcgact
    27601 tgcaacaaac tgtaaaatac gctgaccaag catacgactt catgcgtgat gcagcagcta
    27661 acgatgcagt tgtattgttc gttggtacta agaaacaagc agctgatgca gttgctgaag
    27721 aagcagtacg ttcaggtcaa tacttcatca accaccgttg gttgggtgga actcttacaa
    27781 actggggaac aatccaaaaa cgtatcgctc gtttgaaaga aattaaacgt atggaagaag
    27841 atggaacttt cgaagttctt cctaagaaag aagttgcact tcttaacaaa caacgtgcgc
    27901 gtcttgaaaa attcttgggc ggtatcgaag atatgcctcg tatcccagat gtgatgtacg
    27961 tagttgaccc acataaagag caaatcgctg ttaaagaagc taaaaaattg ggaatcccag
    28021 ttgtagcgat ggttgacacc aatactgatc cagatgatat cgatgtaatc atcccagcta
    28081 acgatgacgc tatccgtgct gttaaattga tcacagctaa attggctgac gctattatcg
    28141 aaggacgtca aggtgaggat gcagtagcag ttgaagcaga atttgcagct tcagaaactc
    28201 aagcagattc aattgaagaa atcgttgaag ttgtagaagg tgacaacgct taatttatac
    28261 aaatagtaat tacctaggag ggcggggctt agcccggctc tcctattttc aaaaaatata
    28321 ggagaattaa aatggcagaa attacagcta aacttgtaaa agagttgcgt gaaaaatctg
    28381 gtgccggtgt tatggacgct aaaaaagcgc ttgtagaaac agacggtgac atcgaaaaag
    28441 cgattgaatt gcttcgtgaa aaaggtatgg ctaaggcagc taagaaagct gaccgtgttg
    28501 ctgcagaagg tttgactggt gtttatgtta acggtaatgt tgcagcagtt attgaagtaa
    28561 acgctgaaac tgacttcgtt gcaaaaaacg ctcaattcgt tgaattggta aatactacag
    28621 ctaaagtcat tgctgaagga aaacctgcta acaatgaaga agctcttgct ttgataatgc
    28681 cttcaggtga aactcttgaa gctgcatacg tatctgcaac agcaactatc ggagagaaaa
    28741 tctcattccg tcgctttgca ttgattgaaa aaacagacgc acaacacttt ggagcatacc
    28801 aacataacgg tggacgtatc ggtgttattt cagttgttga aggtggagac gaagcacttg
    28861 ctaaacaatt gtcaatgcac atcgcagcga tgaaaccaac agttctttct tacaaagaat
    28921 tggatgagca attcgttaaa gatgagttgg cacaattgaa tcacgttatc gaccaagaca
    28981 acgaaagccg tgcaatggtt aataaaccag ctcttccaca cttgaagtat ggatcaaaag
    29041 ctcaattaac tgatgatgtt attgctcaag ctgaagctga catcaaagct gaattggctg
    29101 cagaaggcaa accagaaaaa atctgggaca aaattattcc aggtaaaatg gatcgcttca
    29161 tgcttgataa cactaaagtt gaccaagctt acacacttct tgcacaagtt tacatcatgg
    29221 atgacagcaa gacagttgaa gcataccttg aatcagttaa cgcttcggta gttgagtttg
    29281 ctcgctttga agttggtgaa ggtatcgaga aagctgcaaa cgacttcgaa gctgaagttg
    29341 cagctacaat ggcagcagcc ttgaataact aataatagaa aggaagcaaa ttgcttcctt
    29401 ttttgtatag ggcttagatt tttcctgaaa ttgttttttc cccagtctca ctcgattaga
    29461 ataattaaaa caaaaagccc tataataagg gctaagggca tttaggagac tatacttcaa
    29521 attcatagta agcgcgttca taacaaggta tctatcctaa ttccccaact tccagtatac
    29581 tagataaaaa ataattatcg gaggaaaggt attgtactat gataattcaa ctaagtgatt
    29641 taggtcaagt tcaccttgtt tgtagcaaga tagatatgag gcagggaata gactcattag
    29701 cctatgtagt taaaacccac tttgaattgg atcctttctc cggtcaagtc tttctctttt
    29761 gtggtggacg taaagaccgc tttaaagccc tttactggga tggtcaagga ttttggctac
    29821 tatataaacg ctttgagaac gtcagactga cttggcccag tacagaaaag gatgtcaaag
    29881 ctctcacacc tgaacaagta gactggctta tgaagggctt ttctatcact ccaaaaatat
    29941 agtagattga aactagaata gtacacctct gcttctaaaa cattgttaga aatcgatttg
    30001 actgtcctga tcgatttgtc ctgttcttat ttcattttac tataaatcca tcagaaagtc
    30061 gtgatttcta ttgaaatgag gactttcttt ttatactcat ctgttttcaa aaagcattct
    30121 agtccatctc cgattaacga tggactttat cacctccttc tccagtcctt gtataacatc
    30181 ttggagttga ttcatgacat cttccaaagt ttgaaaggtt ttattcttaa atccacgttt
    30241 acgaatctct ttccacactt gttcaatggg gttcatctct ggtgtgtatg gaggaataaa
    30301 tgcaaagcca atattagtcg gaatctttaa ggtacttgat ttatgccata tagcattgtc
    30361 cataacgagt aaaagataat catctggata agcttgtgaa atctcctatt cctaaagccc
    30421 ctttagcgca taactttggc tcagcttcta ttatcgctca caccatccat cagaagttta
    30481 atctgaaggt acccaattat cgccaagaag aagattgggc taggatgggt ttaccaatca
    30541 cacgtaagga aatctctaat cggcatatca agacgagtca atactatttg gagccccttt
    30601 ataacctctt gcgagagaga ctattgactc agcccttact tcatgcggat gaaacttctt
    30661 atagggtgct agagagtgat agtcagctga cttactattg gacttttttg tctgggaaag
    30721 ctgagaaaca agggattacg ctttaccatc atgtcctaat cgatttgttc atatcttatt
    30781 tcaatccatt ataaatagtg agaaatatct atcctatctt ctagaatgtc ttccaaacgt
    30841 ggaaactctc gtaaacaaag aggttttaga ggcctattta ccatggacta aagttgtaca
    30901 agaaaagtgc aaataagaaa tctccagatt aggaactatc cgtgagttct ctagtctgga
    30961 gatttttcaa tagacttcgt tattggacgg ttacaattca taaagtgctg tagagagata
    31021 acgttcaccg ttatctggtg ctagggcaag gactttttta cctgtaccta attttttggc
    31081 aacctcgatg gctccgtaga tagctgcagc tgaggaaatc cctacaagga agccttcttt
    31141 tccaccaatt tcacgtccga gtgcaagagc gtcatctgat gttacacgaa cgataccatc
    31201 ataggcttta gtatcaagtg tatcaggaat aaatccagct gagatacctt gaattttgtg
    31261 aggaccaggt ttttcaccag atagaatagc agattcatct gcttctactg caaaaacttg
    31321 aatgttagaa ttttctgatt tgagtgcatg agaaacacca gaaatcgttc caccagtacc
    31381 tactccagca acaaaggcat ctaatccatc tttaccgaaa gcagctagta tctcagctcc
    31441 tgttgttctt tcgtgtactt ctggattagc tggattgtca aattgaagag gaaggaaacc
    31501 atcacgttca gcagcgattt cttgagcctt agcaatagca cctttcattc cctcgctacc
    31561 aggagttagg acgagttcag caccataagc ttggataatt ttacgtcgtt ctacactcat
    31621 agtttcaggc ataacgatga cgactttata ccctttagca gcacctaccc atgaaagtcc
    31681 aataccggtg tttccacttg ttgcttcaac aatagtagaa ccaagtttca gaataccatc
    31741 ttgttcagct ttttcaatca tgctaagggc aatacggtct tttacagatg aaccaggatt
    31801 aaatgcttca agctttatat agacgtctgc agcaccttct ggcacgatgt tgttaagttt
    31861 aacaatcggt gtttgaccga ttaattcagt aatgttgtta taaatagaca tatgaatacc
    31921 tctttgtata agatatcttt agtataacgc tatccatacc atttgtaaaa tatagaaatc
    31981 ctatcgaagc gataggattt ctatatatca tagatcaaag tcattaattt tcaagtgatt
    32041 gcgttcagtt aattctagta aataggtgtg aaggaaaacg atatcaggtt tcgtttgaat
    32101 tgcttcattt tctagtatat tttggtacgc atctttttat tttgctcgct aaagtagttg
    32161 tgagaatgtc aacgaaacgc tgtattattc attgtttaca ctaagtttac aggaacttca
    32221 acctctcgta aaccttggtc agttaaagtg acttttccat taaaaaactc cacaagtgca
    32281 gctttaatag tttctttttc ttctttatca acataaatca tcgtatcgac ttgatctgta
    32341 aagtttgtat ccagctccat gagatcatgt tctttaagga agttactgta ctcttggtac
    32401 tgagcataag acatttgaat agcaatgcca gcctgttctt ttatttcaat aataccaatt
    32461 tctttgacag ctaaggcgac actgccggcg taagcacgaa ttagtcctcc agcgcctagt
    32521 ttaataccac caaagtagcg tgtcacgacc acacagacat tggtgagatt gtgattttct
    32581 agtaccccaa gcatgggaac accagcagta ccactaggct caccatcatc acttgtacgt
    32641 ttaatttcac tacgttctcc aataataaag gcagagcagt tatgtgtcgc tttgtagtgt
    32701 tcttttttga tggcagtaat gaagtcacga gcctcttctt cgctataaac acgcttggca
    32761 tggcagataa agcgagattt tttgatttct tcttggactt ggccgtcctc tttaattgtc
    32821 ctaaattcca tgatttaatt gtactaaaaa atcatctaaa gtgctagttt ttacgaataa
    32881 agaagtatga aagtaaattt agattatctc ggtcgtttat ttactgagaa tgaattaaca
    32941 gaagaagaac gtcagttggc ggagaaactt ccagcaatga gaaaggagaa ggggaaactt
    33001 ttctgtcaac gttgtaatag cactattcta gcagaatggt atttgcccat cggtgcttac
    33061 tattgtcgag agtgcttgct gatgaagcga gtcagaagtg atcaaacttt atactatttt
    33121 ccgcaggagg attttccgaa gcaagatgtt ctcaaatggc gcggccaatt aactcctttt
    33181 caagagaagg tgtcagaggg actgcttcaa gcagtagaca agcaaaagcc aaccttagtt
    33241 catgcggtaa caggagctgg aaagacagaa atgatttatc aagtagtggc taaagtgatc
    33301 aatgcgggtg gtgcagtgtg tttggctagt cctcgcatag atgtttgttt ggagctgtac
    33361 aagcgcctgc aacaggattt ttcttgcggg atagctttgc tacatggaga atcggaacct
    33421 tattttcgaa caccactagt tgttgcaaca acccatcagt tattgaagtt ttatcaagct
    33481 tttgatttgc tgatagtgga tgaagtagat gcttttcctt atgttgataa tcccacgctt
    33541 taccatgctg tcaagaatag tgtaaaggag aatggattga gaatcttttt aacagcgact
    33601 tcgaccaatg agttagataa aaaggtccgt ttaggagaac taaaaagact gaatttaccg
    33661 agacggtttc atggaaatcc gttgattatt ccaaaaccaa tttggttatc ggattttaat
    33721 cgctacttag acaagaatcg tttgtcacca aagttaaagt cctatattga gaagcagaga
    33781 aagacagctt atccgttact catttttgct tcagaaatta agaaagggga gcagttagaa
    33841 gaaatcttac aggagcaatt tccaaatgag aaaattggct ttgtatcttc tgtaacagag
    33901 gatcgattag agcaagtaca agcttttcga gatggagaac tgacaatact tatcagtacg
    33961 acaatcttgg agcgtggagt taccttccct tgtgtggatg ttttcgtagt agaggccaat
    34021 catcgtttgt ttaccaagtc tagtttgatt cagattggtg gacgagttgg acgaagcatg
    34081 gatagaccga caggagattt gcttttcttc catgatgggt taaatgcttc aatcaagaag
    34141 gcgattaagg aaattcagat gatgaataag gaggctggtc tatgaagtgc ttgttatgtg
    34201 ggcagactat gaagactgtt ttaactttta gtagtctctt acttctgagg aatgatgact
    34261 cttgtctttg ttcagactgt gattctactt ttgaaagaat tggggaagag aactgtccaa
    34321 attgtatgaa aacagagttg tcaacaaagt gtcaagattg tcaactttgg tgtaaagaag
    34381 gagttgaagt cagtcataga gcgattttta cttacaatca agctatgaag gattttttca
    34441 gtcggtataa gtttgatgga gacttcctgt taagaaaagt tttcgcttca tttttaagtg
    34501 aggagttgaa aaagtacaaa gagtatcaat ttgttgtaat tcccctaagt cctgatagat
    34561 atgctaatag aggatttaat caggttgagg gcttggtaga ggcagcaggc tttgagtatc
    34621 tggatttatt agagaaaaga gaagagagag ccagttcttc taaaaatcgt tcagagcgct
    34681 tggggacaga acttcctttc tttattaaaa gtggagtcac tattcctaaa aaaatcctac
    34741 ttatagatga tatctatact acaggagcaa ctataaatcg tgttaagaaa ctgttggaag
    34801 aagctggtgc taaggatgta aaaacatttt cccttgtaag atgaggaaaa aatttgcaaa
    34861 aatagaatga aagcgttata ataaaatcat aaaaacaaaa atgtttagaa agaaggtact
    34921 catatgatta aatatagtat ccgtggtgaa aacctagaag taacagaagc aattcgtgat
    34981 tatgtagttt ctaaactcga aaagatcgaa aagtacttcc aaccagaaca agagttggat
    35041 gcccgaatta acttaaaagt ttatcgtgaa aaaacggcta aagtggaagt aacgattccg
    35101 cttggatcta ttactctccg cgcagaagat gtatctcaag atatgtatgg ttcaattgac
    35161 cttgtaactg ataaaattga acgtcagatt cgtaaaaata aaacaaaaat cgagcgtaaa
    35221 aataaaaata aggtagcaac tggtcaatta tttacagatg ctttggtgga agattcaaat
    35281 attgtccagt ctaaagttgt tcgttcaaaa caaattgatt taaaaccaat ggatttggaa
    35341 gaagcaattc tacaaatgga tttattgggg catgatttct ttatctatgt ggatgttgaa
    35401 gatcagacaa ccaatgtgat ttatcgtcgt gaggatggcg aaattggttt gttagaggtt
    35461 aaagaatctt aatttcaata tgtgtaaagg taggtttact gaattgtaaa ctaccttttt
    35521 cttataattg atagaattac tgattacact tttaaaaagt cgctttttga ggggtattat
    35581 ggtataatgg gtgccaagag gttttgaatg aaaaaatttt atgtaagtcc aatttttcct
    35641 attctagtag gattgattgc gtttggagtc ttatccactt tcattatttt tgttaataat
    35701 aatctgttga cggttttaat tttgtttctt tttgtaggag gctatgtttt tttatttaag
    35761 aaactgagag tgcattatac aaggagtgat gtagaacaga tacagtatgt aaaccaccaa
    35821 gcggaagaaa gtttgacagc tctattggaa cagatgcctg taggtgttat gaaattgaat
    35881 ttatcttctg gagaggttga gtggtttaat ccctatgctg aattgatttt gaccaaggaa
    35941 gatggtgatt ttgatttaga agctgttcaa acgattatca aggcttcagt aggaaatccg
    36001 tctacttatg ccaagcttgg tgagaagcgt tatgctgttc atatggatgc ttcttccggt
    36061 gttttgtatt ttgtagatgt atccagggaa caagccataa cagatgaatt ggtaacaagt
    36121 agaccagtga ttgggattgt ctctgtggat aattatgatg atttggagga tgcaacttct
    36181 gagtcagata ttagtcaaat caatagtttt gtagctaatt ttatatcaga gttttcagaa
    36241 aaatacatga tgttttctcg tcgggtaagt atggatcgat tttatctatt tactgactac
    36301 acggtgcttg agggcttgat gaatgataaa ttttctgtta ttgatgcttt cagagaagag
    36361 tcgaaacaga gacagttgcc cttgacctta agtatgggat tttcttatgg cgatggaaat
    36421 catgatgaga tagggaaagt tgctttgctc aatttgaact tggctgaagt acgtggtggc
    36481 gaccaggtgg ttgttaagga aaacgacgaa acgaaaaatc cagtttattt tggtggtggg
    36541 tctgctgctt caatcaagcg tacacggact cgtacgcgcg ctatgatgac agctatttca
    36601 gataagattc ggagtgtaga tcaggttttt gtagtcggtc acaaaaattt agacatggat
    36661 gctttgggct ctgctgtagg tatgcagttg ttcgccagca atgtgattga aaatagctat
    36721 gctctttatg atgaagaaca aatgtctcca gatattgaac gagctgtttc attcatagaa
    36781 aaagaaggag ttacgaagtt gttgtctgtt aaggatgcaa tggggatggt gaccaatcgt
    36841 tctttgttga ttcttgtaga ccattcaaag acagccttaa cattatcaaa agaattttat
    36901 gatttattta cccaaaccat tgttattgac caccatagaa gggatcagga ttttccagat
    36961 aatgcggtta ttacttatat cgaaagtggt gcaagtagtg ccagtgagtt ggtaacggaa
    37021 ttgattcagt tccagaattc taagaaaaat cgtttgagtc gtatgcaagc aagtgttttg
    37081 atggctggta tgatgttgga tactaaaaat ttcacctcgc gagtaactag tcggacattt
    37141 gatgttgcta gctatctcag aacgcgcgga agtgatagta ttgctatcca ggaaatcgct
    37201 gcgacagatt ttgaagaata tcgtgaggtc aatgaactta ttttacaggg gtgtaaatta
    37261 ggttcagatg tactaatagc agaggctaag gacacgaaat gctatgatac agttgttatt
    37321 agtaaggcag cagatgccat gttagccatg tcaggtattg aagcgagttt tgttcttgcg
    37381 aagaatacac aaggatttat ctctatctca gctcgaagtc gtagtaaact gaatgtacaa
    37441 cggattatgg aagagttagg cggtggaggc cactttaatt tggcagcagc tcaaattaaa
    37501 gatgtaacct tgtcagaagc aggtgaaaaa ctgacagaaa ttgtattaaa tgaaataaag
    37561 gaaaaggaga aagaagaatg aaagtaatct ttttagcaga tgttaaagga aaaggtaaaa
    37621 aaggcgaaat taaggaagta ccaacagggt atgcgcaaaa ctttcttatc aaaaagaatc
    37681 tagccaaaga agcgactgct caagctgtag gtgaacttcg tggtaaacaa aaatcggaag
    37741 aaaaagctca cgctgagatg attgcagaag gaaaagcaat taaagcacaa cttgaagcag
    37801 aagaaactgt tgtagaattt gttgaaaaag ttggtccaga tggtcgtacc tttggttcta
    37861 ttaccaataa gaagattgca gaagaattgc aaaagcaatt tggaattaag attgataaac
    37921 gtcatattca agtacaagct ccgattcgag cggttggttt gattgatgtg ccagtgaaaa
    37981 tctatcaaga tatcacaagt gtaatcaatc ttcgtgtgaa agaaggataa gtttacacct
    38041 tcttgacaag attgtaaaag gaagggaagt ctgatggcag aagtagaaga gttacgagta
    38101 caacctcaag atatcttagc tgagcaatcc gttttagggg ctatctttat tgatgagagt
    38161 aaacttgttt ttgtgcgaga atacattgag tctcgggact tttttaagta tgcccatcgt
    38221 ttgattttcc aagccatggt cgatttatcc gatcgtggtg atgccataga tgcaacaacg
    38281 gttcgtacta tccttgataa tcaaggtgat ttacagaata ttggtggctt gtcttacttg
    38341 gttgagattg tcaattctgt gccaacttct gctaatgcgg agtattatgc taagattgtt
    38401 gcagaaaaag caatgctacg tcgtttaatt gccaagttga cagagtctgt caaccaagct
    38461 tacgaagcgt cacaaccagc tgatgaaatt attgctcagg cagaaaaagg gctgattgat
    38521 gtcagtgaaa atgcaaatcg aagcgggttt aagaacattc gagatgtgtt gaatctcaac
    38581 tttggaaatc tggaagctcg ctcgcaacaa acgaccgata ttacaggtat tgcgacaggt
    38641 tatcgtgatt tggatcatat gacaacagga cttcatgagg aggagttgat tatcttagca
    38701 gctcgtccag cagttggtaa gacagcattt gccttgaata tcgctcagaa tattgggact
    38761 aagttggaca aaacggttgc tattttttca ctcgaaatgg gtgcggaaag cttggtagac
    38821 cgtatgttag ctgcagaagg cttggtggag tcacattcta tccgtacagg gcaattgaca
    38881 gatgaggagt ggcaaaaata tactattgct cagggtaatc tagctaacgc cagtatctat
    38941 atcgatgata cgccaggtat tcggattaca gagattcgtt ctcgttctcg taaattggct
    39001 caagaaactg gaaatcttgg tttgattttg atagactatt tgcaacttat cacgggaact
    39061 ggtcgagaaa atcgtcaaca agaagtttct gaaatttctc gtcagttgaa aatactagcc
    39121 aaggaattga aggttccagt aatcgctctg agtcagcttt ctcgtggtgt agaacaacgt
    39181 caggacaaga gaccggtctt gtctgatatt cgtgaatctg ggtctattga gcaggacgct
    39241 gatatcgtag cttttctcta tcgcgatgac tactatgaac gtggtggtga agaagaggag
    39301 ggtatcccaa ataataaggt ggaagttatt atcgagaaaa accgtagtgg agctcgtgga
    39361 acagtggaat tgattttcca aaaagaatac aataaatttt caagtatctc aaagagggag
    39421 gcataagatg tcagatgcat ttacagatgt agccaagatg aaaaaaatca aagaagaaat
    39481 caaggcacat gagggacaag tcgtagaaat gactttggag aatggtcgta agcgccaaaa
    39541 aaatagattg ggtaagctaa ttgaagttta tccatcccta tttattgtgg agtttgggga
    39601 tgtggaagga gataaacaag ttaatgttta cgttgaatcc tttacttact cagatattct
    39661 tacagaaaag aatttgattc attatcttga ctaaagtgag aaattttctc actttttctt
    39721 ttttctccga ataatttagg tgaaggcaat catcgcttta tattattttt taaggaggaa
    39781 gaatgaaaat tttaccgttt atagcaagag gaacaagtta ttacttgaag atgtcagtta
    39841 aaaagcttgt tcctttttta gtagtaggat tgatgctagc agctggtgat agtgtctatg
    39901 cctattccag aggaaatgga tcgattgcgc gtggggatga ttatcctgct tattataaaa
    39961 atgggagcca ggagattgat cagtggcgca tgtattctcg tcagtgtact tcttttgtag
    40021 cctttcgttt gagtaatgtc aatggttttg aaattccggc agcttatgga aatgcgaatg
    40081 aatggggaca tcgtgctcgt cgggaaggtt atcgtgtaga taatacaccg acgattggtt
    40141 ccattacttg gtctactgca ggaacttatg gtcatgttgc ctgggtgtca aatgtaatgg
    40201 gagatcagat tgagattgag gaatataact atggttatac agaatcctat aataaacgaa
    40261 ttataaaagc aaacacgatg acaggattta ttcattttaa agatttggat ggtggcagtg
    40321 ttgggaatag tcaatcctca acttcaacag gcggaactca ttattttaag accaagtctg
    40381 ctattaaaac tgaaccccta gttagtgcaa ctgtgattga ttactattat cctggagaga
    40441 aggttcatta tgatcaaatt ctcgaaaaag acggctacaa gtggttgagt tatactgcct
    40501 ataatggaag ctatcgttat gttcaattgg aggctgtgaa taaaaatcct ctaggtaatt
    40561 ctgttctttc ttcaacaggt ggaactcatt attttaagac caagtctgct atcaaaactg
    40621 aacccctagt tagtgcaact gtgattgatt actattatcc tggggagaag gttcattatg
    40681 atcagatact tgaaaaagac ggctacaagt ggttgagtta tacggcttat aacggaagtc
    40741 gtcgctatat acagctagag ggagtgactt cttcacaaaa ttatcagaat caatcaggaa
    40801 atatctctag ctatggatcc aataatagtt caactgtcgg ttggaagaaa ataaatggta
    40861 gttggtatca tttcaaatca aatggttcta aatcaacagg atggctgaaa gatggttcta
    40921 gctggtatta tttgaaatta tctggtgaaa tgcagacagg atggttaaag gagaatggct
    40981 cgtggtatta tctgggtagt tcaggggcaa tgaaaacagg ctggtaccag gtctctggta
    41041 agtggtatta ttcttactct tcaggcgcct tagctgttaa tacgacggtg gatggctaca
    41101 gagtaaacag tgatggagaa cgagtataga aaattggagt aggagaaatt tcctgctctt
    41161 ttctgtaagc agtttccttg acatttttct tattttgcgc tatcatatat acatataata
    41221 tatgggagtc tgtgtacagt ctgagaggaa gtgttaaact tcgaccgcac ctgatctggg
    41281 taatgccagc ggagggaacg atacttagtc taattttgca ccttttccat gtatggtaaa
    41341 ggtttttctt tttttaaaaa ggaaaacgag aagaggaggt tcttatgaaa gcaagcattg
    41401 ccttgcaagt tttaccccta gcacagggga ttgatcggat agctgttatt gatcaggtca
    41461 ttgcttatct gcaaactcaa gaagtgacga tggtagtgac accatttgaa acggtcttgg
    41521 aaggggagtt tgatgagctt atgcgcattc taaaagaagc gctggaagtg gcagggcagg
    41581 aggcagacaa tgtctttgcc aatgtcaaaa taaatgtagg agagatttta agtattgatg
    41641 agaaacttga gaagtatact gagacgccac attagtctat tgggctttct cggagtcttg
    41701 tcaatctggc agttagcagg ttttcttaaa cttctcccca agtttatcct gccgacacct
    41761 cttgaaattc tccagccctt tgttcgtgac agagaatttc tctggcacca tagctgggcg
    41821 accttgagag tggctttact ggggctgatt ttgggagttt tgattgcctg tcttatggct
    41881 gtgctcatgg atagtttgac ttggctcaat gacctgattt accctatgat ggtggttatt
    41941 cagaccattc cgaccattgc catagctcct atcctggtct tgtggctagg ttatgggatt
    42001 ttgcccaaga ttgtcttgat tatcttaacg acaacttttc ccatcatcgt cagcattttg
    42061 gacggtttta ggcattgcga caaggatatg ctgaccttgt ttagtctgat gcgggccaag
    42121 ccttggcaaa tcctgtggca ttttaaaatt ccagtcagcc tgccctactt ttatgctggt
    42181 ctgagggtca gtgtctccta cgcttttatc acaacagtgg tatctgagtg gttgggaggt
    42241 tttgaaggtc ttggtgttta tatgattcag tctaaaaaac tgtttcagta tgataccatg
    42301 tttgccatta ttattctggt gtcgattatc agtcttttgg gtatgaagct ggtcgatatc
    42361 agtgaaaaat atgtgattaa atggaaacgt acttaaaata gagcgttttt gaaaaagaaa
    42421 agaggaaatc aaaatgaaga aaacatggaa agtgttttta acgcttgtaa cagctcttgt
    42481 agctgttgtg cttgtggcct gtggtcaagg aactgcttct aaagacaaca aagaggcaga
    42541 acttaagaag gttgacttta tcctagactg gacaccaaat accaaccaca cagggcttta
    42601 tgttgccaag gaaaaaggtt atttcaaaga agctggagtg gatgttgatt tgaaattgcc
    42661 accagaagaa agttcttctg acttggttat caacggaaag gcaccatttg cagtgtattt
    42721 ccaagactac atggctaaga aattggaaaa aggagcagga atcactgccg ttgcagctat
    42781 tgttgaacac aatacatcag gaatcatctc tcgtaaatct gataatgtaa gcagtccaaa
    42841 agacttggtt ggtaagaaat atgggacatg gaatgaccca actgaacttg ctatgttgaa
    42901 aaccttggta gaatctcaag gtggagactt tgagaaggtt gaaaaagtac caaataacga
    42961 ctcaaactca atcacaccga ttgccaatgg cgtctttgat actgcttgga tttactacgg
    43021 ttgggatggt atccttgcta aatctcaagg tgtagatgct aacttcatgt acttgaaaga
    43081 ctatgtcaag gagtttgact actattcacc agttatcatc gcaaacaacg actatctgaa
    43141 agataacaaa gaagaagctc gcaaagtcat ccaagccatc aaaaaaggct accaatatgc
    43201 catggaacat ccagaagaag ctgcagatat tctcatcaag aatgcacctg aactcaagga
    43261 aaaacgtgac tttgtcatcg aatctcaaaa atacttgtca aaagaatacg caagcgacaa
    43321 ggaaaaatgg ggtcaatttg acgcagctcg ctggaatgct ttctataaat gggataaaga
    43381 aaatggtatc cttaaagaag acttgacaga caaaggcttc accaacgaat ttgtgaaata
    43441 atgacagaaa ttagactaga gcacgtcagt tatgcctatg gtcaggagag gattttagag
    43501 gatatcaacc tacaggtgac ttcaggcgaa gtggtttcca tcctaggccc aagtggtgtt
    43561 ggaaagacca ccctctttaa tctaatcgct gggattttag aagttcagtc agggagaatt
    43621 gtccttgatg gtgaagaaaa tcccaagggg cgcgtgagtt atatgttgca aaaggatctg
    43681 ctcttggagc acaagacggt gcttggaaat atcattctgc ccctcttgat tcaaaaggtg
    43741 gataaggcag aagctatttc ccgagcggat aaaattcttg cgaccttcca gctgacagct
    43801 gtaagagaca agtatcctca tgaacttagc ggtgggatgc gccagcgtgt agccttactc
    43861 cggacctacc tttttgggca caagctcttt ctcttagatg aggcctttag cgccttggat
    43921 gagatgacaa agatggaact ccacgcttgg tatcttgaga ttcacaaaca gttgcagcta
    43981 acaactctga tcatcacgca tagtattgag gaggccctca atctcagcga ccgcatctat
    44041 atcttgaaaa atcgccctgg gcagattgtt tcagaaatta aactagattg gtctgaagat
    44101 gaggacaagg aagtccaaaa gattgcctac aaacgtcaaa tcttggcaga attaggctta
    44161 gataagtaga aaaataggga gttggtgaag attatccttt accagcgccc tttttctttt
    44221 aaaaatgaga aaatttcggt ataatagtca aacaaggtca aggtttaaag agagaggtgg
    44281 gtttgttatg agatttaaaa atacatcgga tcatattgag gcctacatca aggcgatttt
    44341 agatcaatct ggtatcgtgg agttgcaacg gagtcagttg gcagatacct ttcaggttgt
    44401 tcctagtcag attaactacg tgatcaagac acgctttacg gaaagtagag gctacttggt
    44461 tgaaagtaag cgtggtggcg gaggctacat tcgtatagga cggattgagt tttctagtca
    44521 tcatgaaatg ctccgggatc tgctttactc gattggtgag cgagtcagtc aagaaattta
    44581 tgaggatatt ctccagcttt tggttgagca ggaattgatg accaagcagg agatgaattt
    44641 gctagaatca gtagctttgg atcgcgtttt aggagaagaa gctccagttg ttcgagcaaa
    44701 catgctacgt caggtcatac aagaggtaga tagaaaaggg aagtaagatg aactattcaa
    44761 aagcattgaa tgaatgtatc gaaagtgcct acatggttgc tggacatttt ggagctcgtt
    44821 atctagagtc gtggcacttg ttgattgcca tgtctaatca cagttatagt gtagcagggg
    44881 caactttaaa tgattatccg tatgagatgg accgtttaga agaggtggct ttggaactga
    44941 ctgaaacgga ctatagccag gatgaaacct ttacggaatt gccgttctcc cgtcgtttgc
    45001 aggttctttt tgatgaagca gagtatgtag cgtcagtggt ccatgctaag gtactaggga
    45061 cagagcacgt cctctatgcg attttgcatg atggcaatgc cttggcgact cgtatcttgg
    45121 agagggctgg tttttcttat gaagacaaga aagatcaggt caagattgct gcccttcgtc
    45181 gaaatttaga agaacgtgca ggctggactc gtgaagatct caaggcttta cgccaacgcc
    45241 atcgtacagt agctgacaag caaaattcta tggccaatat gatgggcatg ccgcagactc
    45301 ctagtggtgg tctcgaggac tatacgcatg atttgacaga gcaagcgcgt tctggcaagt
    45361 tagaaccagt catcggtcgg gacaaggaaa tctcgcgtat gattcaaata ttgagccgta
    45421 agactaagaa caaccctgtc ttggttgggg atgctggtgt cgggaaaaca gctctggccc
    45481 ttggtcttgc ccagcgtatt gctagtggtg acgtgcctgc ggaaatggct aagatgcgcg
    45541 tgttagaact tgatttgatg aatgtcgttg cagggacacg cttccgtggt gactttgaag
    45601 aacgcatgaa taatatcatc aaggatattg aagaagatgg ccaagtcatc ctctttatcg
    45661 atgaactcca caccatcatg ggttctggta gcgggattga ttcgactctg gatgcggcca
    45721 atatcttgaa accagccttg gcgcgtggaa ctttgagaac ggttggtgcc accactcagg
    45781 aagaatatca aaaacatatc gaaaaagatg cggctctttc tcgtcgtttc gctaaagtga
    45841 cgattgaaga accaagtgtg gcagatagta tgactatttt acaaggtttg aaggcgactt
    45901 atgagaaaca tcaccgtgta caaatcacag atgaagcggt tgaaacagcg gttaagatgg
    45961 ctcatcgtta tttaaccagt cgtcacttgc cagactctgc tatcgacctc ttggacgaag
    46021 cagcagcaac agtgcaaaat aaggcaaagt atgtaaaagc agacgattca gatttgagtc
    46081 cagctgacaa ggccctgatg gatggcaagt ggaaacaggc agcccagcta atcgcaaaag
    46141 aagaggaagt acctgtctac aaagacttgg tgacagagtc tgatattttg accaccttga
    46201 gtcgcttgtc aggaatccca gttcaaaaac tgactcagac tgacgctaag aaatatctaa
    46261 atcttgaagc agaactccat aaacgggtta tcggtcaaga tcaagctgtt tcaagtatta
    46321 gtcgtgcgat tcgccgtaat cagtcaggaa ttcgcagtca caagcgtccg atcggttcct
    46381 ttatgttcct aggacccaca ggtgtcggta agaccgagtt ggccaaggct ttggcagaag
    46441 ttctctttga tgacgaatca gcccttatcc gctttgatat gagtgagtat atggagaaat
    46501 tcgcagccag ccgtctaaat ggagctcctc caggctatgt gggttacgaa gaaggtgggg
    46561 agttgacaga gaaggttcgc aataaaccct attccgttct cctctttgat gaggtagaga
    46621 aggctcaccc agatatcttt aatgttctct tgcaggttct ggatgacggt gtcttgacag
    46681 atagcaaggg acgcaaggtc gatttttcaa ataccattat cattatgaca tcgaacctag
    46741 gtgcgactgc ccttcgtgat gataagactg ttggttttgg ggctaaggat attcgttttg
    46801 accaggaaaa tatggaaaaa cgcatgtttg aagaactgaa aaaagcttat agaccggaat
    46861 tcatcaaccg tattgatgag aaggtggtct tccatagcct atctagtgac catatgcagg
    46921 aagtggtgaa gattatggtc aagcctttag tggcaagttt ggctgaaaaa ggcattgact
    46981 tgaaattaca agcttcagct ctgaaattgt tagcaaatca aggatatgac ccagagatgg
    47041 gagctcgccc acttcgcaga accctgcaaa cagaagtgga ggacaagttg gcagaacttc
    47101 ttctcaaggg agatttagtg gcaggcagca cacttaagat tggtgtcaaa gcaggccagt
    47161 taaaatttga tattgcataa aagaataaaa gtatcagcat ctgaccataa gtcacagtgg
    47221 agtgaaactc aatgaaaatc aaagaacaaa ctaggcagct agccgcaggt tgctcaaaac
    47281 actgttttga ggttgtagat agagctgacg tggtttggag agattttcga agagtataag
    47341 ctgcaagatg aatgattttc ttgtattgac gttgttgttg acaaaaagta gcggataaat
    47401 gaaatccatt ccattatcat agatgatagg ctggtaggaa attttcaaat agcatacagg
    47461 aaatagatgt atggagttct ggtagtagaa agggagagag atgaacattt tagttgcaga
    47521 tgacgaggaa atgattagag aaggaattgc agcatttctg acagaagagg gttatcatgt
    47581 cattatggct aaggatggac aagaggtctt ggaaaaattt caagatctcc ctatccatct
    47641 catggtattg gatttaatga tgcctaggaa gagtggtttt gaagtgttaa aagaaatcaa
    47701 tcaaaagcac gatattcctg tcatcgtctt gagtgctctg ggagatgaaa ctactcagtc
    47761 acaggtattt gatctctatg ctgatgatca tgtgacaaaa cctttttctt tggtactgct
    47821 tgtcaagcgt attaaggcgc ttatcagacg ttactacgtc atagaggata tttggcgata
    47881 tcaggatgta acagtggatt ttacctctta caaagcacat tataaaaatg aagaaattga
    47941 tctcaaacca aaggaattac tggtactaaa gtgtttgatt cagcataaaa atcaagtttt
    48001 aagtagagag cagatattgg aagaaatttc aaaagatgta gctgatttac cttgtgatag
    48061 ggtcgttgat gtctatattc gtactcttcg caaaaaatta gctttagatt gtatcgtgac
    48121 tgtgaaaaat gttgggtata agattagctt atgataaaaa atcctaaatt attaaccaag
    48181 tcttttttaa gaagttttgc aattctaggt ggtgttggtc tagtcattca tatagctatt
    48241 tatttgacct ttccttttta ttatattcaa ctggaggggg aaaagtttaa tgagagcgca
    48301 agagtgttta cggagtattt aaagactaag acatctgatg aaattccaag cttactccag
    48361 tcttattcaa agtccttgac catatctgct caccttaaaa gagatattgt agataagcgg
    48421 ctccctcttg tgcatgactt ggatattaaa gatggaaagc tatcaaatta tatcgtgatg
    48481 ttagatatgt ctgttagtac agcagatggt aaacaggtaa cagtgcaatt tgttcacggg
    48541 gtggatgtct acaaagaagc aaagaatatt ttgcttttgt atctcccata tacatttttg
    48601 gttacaattg ctttttcctt tgttttttct tatttttata ctaaacgctt gctcaatcct
    48661 cttttttaca tttcagaagt gactagtaaa atgcaagatt tggatgacaa tattcgtttt
    48721 gatgaaagta ggaaagatga agttggtgaa gttggaaaac agattaatgg tatgtatgag
    48781 cacttgttga aggttattca tgagttggaa agtcgtaatg agcaaattgt aaaattgcaa
    48841 aatcaaaagg tttcctttgt ccgcggagca tcacatgagt tgaaaacccc tttagccagt
    48901 cttagaatta tcctagagaa tatgcagcat aatattggag attacaaaga tcatccaaaa
    48961 tatattgcaa agagtataaa taagattgac cagatgagcc acttattgga agaagtactg
    49021 gagtcttcta aattccaaga gtggacagag tgtcgtgaga ccttgactgt taagccagtt
    49081 ttagtagata ttttatcacg ttatcaagaa ttagctcatt caataggtgt tacaattgaa
    49141 aatcaattga cagatgctac cagggtcgtc atgagtctta gggcattgga taaggttttg
    49201 acaaacctga ttagtaatgc aattaaatat tcagataaaa atgggcgcgt aatcatatcc
    49261 gagcaagatg gctatctctc tatcaagaat acatgtgcgc ctctaagtga ccaagaacta
    49321 gaacatttat ttgatatatt ctatcattct caaatcgtga cagataagga tgaaagttcc
    49381 ggtttgggtc tttacattgt gaataatatt ttagaaagct atcaaatgga ttatagtttt
    49441 ctcccttatg aacacggtat ggaatttaag attagcttgt agacagatta gttttttatt
    49501 aaagttcata tagggttaac ataagtgtgt tattctttgt gtagataaaa gaaaggatac
    49561 taatatggta ttagcgatta ttttagtaac attctttatt cgattgattt ttttaaagcg
    49621 ttcgatagag aatgagaaac gaatccttag caatggcggg aaagaatttg gagttgagaa
    49681 tacaaaacga ttaactatgg ctcatattgt tttttatctc tcttgcttgg ttgaggcaat
    49741 ggtgcacaag acaatttttg atggcatggg catggttggt ttagtcttgc ttattttttc
    49801 tatgctgatg ttgatgttgg tgattcactt gttgggagat atttggacag tgaagcttat
    49861 gcttgtcaat aatcacaaat atgtagatca tatcttgttt aggacagtaa aacatcctaa
    49921 ttacttttta aatattcttc ctgagttgat tggcttgacc ttgttgagtc atgcttatgt
    49981 gacttttgtt ttagtttttc cagtttatgc agttattttg tatcgacgaa tagctgaaga
    50041 ggaaaagcta ttacatgaag ttataatccc aaatggaagc ataaagagat aaatacaaaa
    50101 ttcgatttat atacagttca tattgaagtg atatagtaag gttaaagaaa aaatatagaa
    50161 ggaaataatt atgtttgcat caaaaagcga aagaaaagta cattattcaa ttcgtaaatt
    50221 tagtgttgga gtagctagtg tagtagttgc tagcttgttc ttaggaggag tagtccatgc
    50281 agaaggggtt agaagtgggg atacccccaa ggttacatct agtgggcaag atatatcgaa
    50341 gaagtatgct gatgaagtca agtcgcatct agaaaaaata ttgagtgaga tccaattaga
    50401 taaaagaaaa catacccaaa atctcgcctt caacaaaaag ttgagcagaa ttcaaacgga
    50461 gtatttctat ttaaaaaaga agttgaaagc tgagttgacg tcaaaaacaa aagaagagtt
    50521 aacgtcaaaa acagaagagt tgacgtcaaa aacaaaaaaa gagttagacg cagcttttga
    50581 gcagtttaaa aaagatacat tatcaacaga accagaaaaa aaggttgcag aagctgagaa
    50641 gaaggtagca gaagctaaga aaaaagccga ggatcaaaaa gaagaagatc gtcgtaacta
    50701 cccaaccaat acttacaaaa cgcttgaact tgaaattgct gagttcgatg tgaaagttaa
    50761 agaagcggag cttgaactat taaaagagaa agctaaggaa tctcgagacg aggaaaaaat
    50821 taagcaagca gaagcggaag ttgagagtaa acaagctgag gctacaaggt tagaaaacat
    50881 caagacagat cgtaaaaaag cagaagaaga agctaaacga aaagcagatg ctaagttgaa
    50941 ggaagctaat gtagcgactt cagatcaagg taaaccaaag gggcgggcaa aacgaggagt
    51001 tcctggagag ctagcaacac ctgataaaaa agaaaatgat gcgaagtctt cagattctag
    51061 cgtaggtgaa gaaactcttc caagctcatc cctgaaatca ggaaaaaagg tagcagaagc
    51121 tgagaagaag gttgaagaag ctgagaaaaa agccaaggat caaaaagaag aagatcgccg
    51181 taactaccca accaatactt acaaaacgct tgaacttgaa attgctgagt ccgatgtgaa
    51241 agttaaagaa gcggagcttg aactagtaaa agaggaagct aaggaacctc gagacgagga
    51301 aaaaattaag caagcaaaag cggaagttga gagtaaacaa gctgaggcta caaggttaga
    51361 aaaaatcaag acagatcgta aaaaagcaga agaagaagct aaacgaaaag cagcagaaga
    51421 agataaagtt aaagaaaaac cagctgaaca accacaacca gcgccggctc ctcaaccaga
    51481 aaaaccagct ccaaaaccag aaaaaccagc tccagctcca aaaccagaga atccagctga
    51541 acaaccaaaa gcagaaaaac cagcacaacc atctactcca aaaacaggct ggaaacaaga
    51601 aaacggtatg tggtacttct acaatactga tggttcaatg gcgacaggat ggctccaaaa
    51661 caatggctca tggtactacc tcaacagcaa tggcgctatg gcgacaggat ggctccaaaa
    51721 caatggttca tggtactatc taaacgctaa tggttcaatg gcaacaggat ggctccaaaa
    51781 caatggttca tggtactacc tcaacgctaa tggttcaatg gcgacaggat ggctccaaaa
    51841 caatggttca tggtactacc tcaacgctaa tggtgatatg gcgacaggat ggctccaaaa
    51901 caatggctca tggtactacc taaacgctaa tggtgatatg gcgacaggat ggctccaaaa
    51961 caacggttca tggtactacc taaacagcaa tggtgctatg gtaacaggat ggctccaaaa
    52021 caatggctca tggtattacc tcaacgctaa tggtgatatg gcgacaggtt gggtgaaaga
    52081 tggagacacc tggtactatc ttgaagcatc aggtgctatg aaagcaagcc aatggttcaa
    52141 agtatcagat aaatggtact atgtcaatgg ctcaggtgcc cttgcagtca acacaactgt
    52201 agatggctat ggagtcaatg ccaatggtga atgggtaaac taaacctaat ataactagtt
    52261 aatactgact tcctgtaaga actttttaaa gtattcccta caaataccat atcctttcag
    52321 tagataatat acccttgtag gaagtttaga ttaaaaaata actctgtaat ctctagccgg
    52381 atttatagcg ctagagacta cggagttttt ttgatgagga aagaatggcg gcattcaaga
    52441 gactctttaa gagagttacg ggttttaaac tattaagcct tctccaattg caagagggct
    52501 tcaatctctg ctagggtgct agcttgcgaa atggctccac ggagtttggc agcgccagat
    52561 gttccacgga gatagtgagg agcgaggccg cggaattcac gaactgcgac gttttctcct
    52621 ttgaggttaa tcaatcgttt caagtgttcg taggcgatct tcatcttgtc ttcaaaggtc
    52681 aaatcaggta ggatttctcc tgtttcaaag taatggttga tttggttgaa gaggtaagga
    52741 tttcccatgg cagctcggcc aatcatgact gcgtcagcac caacttcttc gatgcgttgc
    52801 ttggcttctt ggacagtacg gatatcaccg ttggcgatga atggaatctt ggttagagct
    52861 tgggcaacct tgtaaagggt ctcaaggtct gcgtggccag tatacatttg ttcacgggta
    52921 cggccatgca tggcgagggc agaaacacct gcagcttcag cagcgagggc attttctact
    52981 gccagagatg ggtccgccca gccggtacgc attttgacag taagtgggat atcaaggaca
    53041 gactggacct tgttgatgat ggagtaaatc ttgtctggat ccttgagcca catagcacca
    53101 gcttcgttct tcacgatttt gttgacaggg cagcccatgt tgatatcgac gatatcggtc
    53161 ttggtgtttt cttggatgaa ttctgctgcg cgtgctaggc tgtcttcatc gctaccaaaa
    53221 agttggatag agacagggtt ttcgccctca tcgatatgaa gcatgtgcag ggttttttcg
    53281 ttgttgtatt ggattccctt gtcagagacc atttccatta caacgagtcc agctccgagc
    53341 tcttttgcga tggtacgaaa ggctgagttg gtcacgccag ccataggcgc taaaacggta
    53401 cgattgggaa tctcaacatt gccaatcata aaaggtgtat taagatttgt cacgaatgag
    53461 ttcctccagg tccttttcat caaagttgta agtagtttgg cagaattgac aagtgatttc
    53521 tgccccgtgg tcttcttctt tcatttcctg taagtctgag cttggaaggc tggcaagagc
    53581 gttcataaag cgttcatggc tacagtcaca ttggaaacgg atttcttctt cagaaagacg
    53641 cttgtaggct tcgtccccgt agatagcctt gaggagggct tcgatatggt cgtcgctttc
    53701 gagaagagta gagatagctg gcatttcttg gatgcgtttt tcaaagcgag caatctcttc
    53761 tttcttggct cctggcaaga cttgaactag gaaaccacct gcaaccttga ccttgtcttc
    53821 ctcgtccaaa aggacattga ggccgaccgc tgaaggcgtt tgttggcttt cagtaaggta
    53881 aaaggcaagg tcttcaccga tttctccaga gatgagggga gttatagagt tgtaaggatt
    53941 tccagtaccg tagtctgtga taacgaggaa ttgaccattt ccaacaaaag gtccgactag
    54001 gacttcacca gtcgcagtct ttttgatgtc aacaccagga ttttgaacgt agcctttgac
    54061 gttccccttg gtatcagcga cggtgataat agcacctaga gagctagatc ccaacacctt
    54121 aactgtaagt ttggtatttc ctttttcatt ggctgcgaga atctggctgg cgataagagt
    54181 tcgaccaagc gctacagttg agctagcttg ggtttgatgt ttttcttgag cagtgcggac
    54241 ggtttcagtg ctatcaagga caaaagcacg aaaggctccg ctttctgata tagttttaat
    54301 aattttatcc atagctacta ttttagcata aaaatgccca aagggggagc cgtgtgttta
    54361 ctgattttca ggataatgga ccaggaaatc agcatgaaaa taaaaagaga aacagattat
    54421 tttagcattt gtaagattta tgctatgctt aaggtagaaa atgaaagggg taacaaatgt
    54481 atttaggaga tttgatggag aaagccgagt gtggtcaatt ttcaatcctt tcctttctat
    54541 tacaagagtc tcagacgacc gtcaaggctg taatggaaga aacaggattt tcaaaagcaa
    54601 ccctaaccaa atatgtcacc ctgctcaatg acaaggcttt ggatagtggc ttagagctgg
    54661 ctattcactc agaagatgaa aatctgcgtc tgtctatcgg tgcagctacc aaggggagag
    54721 atattcggag cttgtttttg gagagtgctg ttaaatacca gattttggtt tatcttctct
    54781 accaccaaca gtttttagcc catcagctgg ctcaagaatt ggtgattagc gaggctacgc
    54841 ttggtcgtca cttggctggt ttaaatcaga ttttgtcaga atttgattta tccatccaaa
    54901 atggacgttg gcgaggtcca gagcatcaga ttcgctattt ctatttctgt cttttccgaa
    54961 aggtctggtc gagtcaggaa tgggaaggtc acatgcagaa accagagaga aaacaggaga
    55021 ttgccaattt agaggaaatc tgcggtgcaa gtttgtctgt ggggcagaaa ttggacttgg
    55081 ttctctgggc tcacatcagt caacaacgtc ttcgggtcaa tgcttgtcag tttcaagtca
    55141 tagaagagaa aatgcgaggg tattttgaca atatcttcta tcttcgtttg ctgagaaagg
    55201 ttccgtcctt ttttgctggg caacatattc cactaggagt tgaggatggt gagatgatga
    55261 tattcttctc ttttctccta tctcatcgca ttcttcctct tcatactatg gaatatattc
    55321 ttggttttgg agggcagttg gcagatttac tgacgcaatt gattcaagaa atgaagaagg
    55381 aggaactatt gggggattat acagaggacc atgtcaccta tgaactgagt cagctttgtg
    55441 ctcaagtcta tctctataag ggctatattt tacaggatcg ctacaagtac cagttagaga
    55501 atcgtcatcc atatttactg atggaacatg attttaaaga gacagcagag gagatttttc
    55561 atgctctacc tgcttttcaa caggggacag atttagataa gaagattctc tgggaatggc
    55621 tccagttaat cgaatatatg gctgaaaacg gtggccagca tatgcggatt ggtctggatt
    55681 tgacatctgg ttttcttgtc ttttcaagga tggcagccat tttgaaacgg tatttggaat
    55741 acaatcgttt tattaccatt gaagcttatg accctagtcg gcattatgat ttgctggtta
    55801 ccaataaccc gattcataag aaggaacaga ccccagtcta ttatttaaaa aatgacttgg
    55861 atatggaaga tttggcaggg attcgtcagt tattattcac ttaaaaggct tggttaatcc
    55921 aggtcttttt tgtgaaattc acacaatctc ctcacatttt tttaaaaatt aaaaaaagtt
    55981 gataaacaag aaagcgcttt attttgtata ctagtaagtg taaagaggaa acacctcaag
    56041 atctttatca ggaggacagt acatgtcaca agaaaaatac atcatggcca ttgaccaggg
    56101 aactacaagt tctcgtgcca tcattttcaa caaaaaaggg gaaaaggtta gctcgagtca
    56161 aaaagagttt acccagattt tccctcaggc aggttgggtt gagcacaatg ccaatgaaat
    56221 ttggaactct gttcagtcag ttattgcggg tgctttcatc gaaagtggtg tcaagccaaa
    56281 tcaaatcgag gcaatcggga ttaccaacca acgtgaaaca acggttgtct gggataagaa
    56341 aacaggactt cctatctaca atgctatcgt ttggcagtca cgccagacag cacctttggc
    56401 tgagcaacta aaaagccaag gttatgtgga aaaattccat gaaaagactg gtttgattat
    56461 tgatgcttac ttctctgcta ccaaggttcg ttggattttg gatcatgtag aaggcgctca
    56521 agagcgagca gaaaaagggg aattgctctt tggtactatc gatacttggt tggtttggaa
    56581 attgactgac ggtgcggctc acgtgactga ctactcaaat gcagctcgta ccatgcttta
    56641 taacattaaa gaactcaaat gggatgatga gattttggaa atccttaaca ttccgaaggc
    56701 tatacttcca gaagttcgtt ctaactccga aatctacggc aagacagctc cattccattt
    56761 ctacggtgga gaggtgccaa tctcaggtat ggctggggac caacaagcag ccctctttgg
    56821 acagttggct tttgagccag gtatggttaa gaatacttat ggaacaggct ctttcatcat
    56881 catgaatact ggggaagaga tgcagttgtc tgaaaacaac ctcttgacaa ccattggtta
    56941 cggaatcaac ggtaaggttt attatgcctt ggaaggttct atcttcatcg caggaagtgc
    57001 tattcagtgg cttcgtgacg gtcttcgcat ggttgaaaat tcaccagaat ctgaaaaata
    57061 cgctcgtgat tctcacaaca acgatgaagt ttatgtcgtt ccagccttta caggtctagg
    57121 cgctccatac tggaaccaaa atgctcgtgg ttccgtcttt ggtttgactc gtggaacaag
    57181 caaagaagac tttatcaagg cgactttgca atctattgct tatcaagtgc gtgacatcat
    57241 cgacaccatg caagtggata ctcagactgc cattcaagta ttgaaggtgg atggtggtgc
    57301 agccatgaac aacttcctca tgcagttcca ggcggatatt ttaggcattg atattgcacg
    57361 tgctaaaaac ttggaaacaa cagctctagg agcggccttc ctagcaggtt tatcagtagg
    57421 gtactggaaa gacttggacg agttgaaact cttgaacgag acaggagaac tctttgagcc
    57481 atctatgaat gaatctcgca aggaacaact ctacaagggc tggaagaagg ctgtgaaagc
    57541 aactcaagtc tttgcggaag tagacgacta atactggcag aataaagcga tttatttaga
    57601 aagtgtgtaa atatggaatt ttcaaagaaa acacgtgaat tgtcaattaa aaaaatgcag
    57661 gaacgcaccc tggacctctt gattatcggt ggaggaatca caggagctgg tgtagccttg
    57721 caggcggcag ctagcggtct tgagactggt ttgattgaaa tgcaagactt tgcagaagga
    57781 acatctagtc gttcaacaaa attggttcac ggaggtcttc gctacctcaa acaattcgac
    57841 gtagaagtgg tctcagatac ggtttctgaa cgtgcagtgg ttcaacaaat cgctccacac
    57901 attccaaaac cagatccaat gctcttacca gtttacgatg aagatggagc aacctttagc
    57961 ctcttccgtc ttaaagtagc catggacttg tacgacctct tggcaggtgt tagcaacaca
    58021 ccaactgcga acaaggtttt gagcaagaaa gaagtcttgg aacgccagcc aaacttgaag
    58081 aaggaaggct tggtaggagg tggagtgtat cttgacttcc gtaacaacga tgcgcgtctc
    58141 gtgattgaaa acatcaaacg tgccaaccaa gacggtgccc tcattgccaa ccacgtgaag
    58201 gcagaaggct tcctctttga cgaaagtggc aagattacag gtgttgtagc tcgtgatctc
    58261 ttgacagacc aagtatttga aatcaaggcc cgtctggtta ttaatacaac aggtccttgg
    58321 agtgataaag tacgtaattt gtctaataag ggaacgcaat tctcacaaat gcgcccaact
    58381 aagggagttc acttggtagt ggattcaagc aaaatcaagg tttcacagcc agtttacttc
    58441 gacacaggtt tgggtgacgg tcgtatggtc tttgttctcc cacgtgaaaa caagacttac
    58501 tttggtacaa ctgatacaga ctacacaggt gatttggagc atccaaaagt aactcaagaa
    58561 gatgtagatt atctacttgg cattgtcaac aaccgctttc cagaatccaa catcaccatt
    58621 gatgatatcg aaagcaactg ggcaggtctt cgtccattga ttgcagggaa cagtgcctct
    58681 gactataatg gtggaaataa cggtaccatc agtgatgaaa gctttgacaa cttgattgcg
    58741 actgttgaat cttatctctc caaagaaaaa acacgtgaag atgttgagtc tgctgtcagc
    58801 aagcttgaaa gtagcacatc tgagaaatat ttggatccat ctgcagtttc tcgtgggtct
    58861 agcttggacc gtgatgacaa tggtcttttg actcttgctg gcggtaaaat cacagactac
    58921 cgtaagatgg ctgaaggagc tatggagcgc gtggttgaca tcctcaaagc agaatttgac
    58981 cgtagcttta aattgatcaa ttctaaaact taccctgttt caggtggaga attgaaccca
    59041 gcaaatgtgg attcagaaat cgaagccttt gcgcaacttg gagtatcacg tggtttggat
    59101 agcaaggaag ctcactatct ggcaaatctt tacggttcaa atgcaccgaa agtctttgca
    59161 cttgctcaca gcttggaaca agcgccaggt cttagcttgg cagacacctt gtcccttcac
    59221 tatgcaatgc gcaatgagtt gactcttagc ccagttgact tccttcttcg tcgtaccaat
    59281 cacatgctct ttatgcgtga tagcttggat agtatcgttg agccaatttt ggatgaaatg
    59341 ggacgattct atgactggac agaagaagaa aaagcaactt accgtgctga tgtaaaagca
    59401 gctcttgctc aaaacgactt agcagaatta aaaaattaag aaaaaataaa agaggtggag
    59461 ggtagcaatc cttgtcgccc gccccttttt taatggagac agaaaaatga tgagtgaatt
    59521 atttggagaa tttttaggga ctttaatcct gattcttcta ggaaatggtg ttgttgcagg
    59581 tgtggttctt ccaaaaacca agagcaacag tgcaggttgg attgtgatta ctatgggttg
    59641 ggggattgca gttgcggttg cagtatttgt atctggaaaa ctcagcccag ctcatttaaa
    59701 tccagctgtg acagttggtg tagctttaaa aggtgacttg ccttgggctt ccgttttgcc
    59761 ttatatctta gcccagttcg caggggctat gctgggtcag attttggttt ggttgcaatt
    59821 caaacctcac tatgaggcag aagaaaatgc aggcaatatc ctggcaacct tcagtactgg
    59881 accagccatc aaggatactg tatcaaactt gattagcgaa atccttggaa cctttgtttt
    59941 ggtgttgaca atctttgctt tgggtcttta cgattttcag gcaggtatcg gaacctttgc
    60001 agtgggaact ttgattgtcg gtatcggtct atcactaggt gggacaacag gttatgcctt
    60061 gaacccagct cgtgaccttg gacctcgtat catgcacagc atcttgccaa ttccaaacaa
    60121 gggagacgga gactggtctt acgcttggat tcctgttgta ggccctgtta tcggagcagc
    60181 cttggcagtg cttgtattct cacttttcta gtttatactc ttcgaaaatc aaattcaaac
    60241 cacgtcagcg tcgccttacc gtactcaagt acagcttgcg gctagcttcc tagtttgctc
    60301 tttgattttc attgagtatt agaaaacaat tatgttgata gagcttgggc aagagcccaa
    60361 tttcagcaaa aaatgaagta aatcttctca taataaaacg catcatatca agcacgaaaa
    60421 ttccacgagg tcaactacag tcagaaagct gaacaacaag ccaaaacgcc caaaaaaggc
    60481 ggcaaaaagc aagcacctgc aagcaacgtg ccgaaatggt caaatcctga ttatgtcaac
    60541 gaattagacc caaaaatcgt tgatatgcta gtagaatttc acaagtcaca aggcactttg
    60601 gaaactcccg aggcgcaagc agaaatcgcc caaaaacgtg aagaaatcga gcaaaggaga
    60661 gctgagcttg aggataaaaa acaagagctt ttgaaccgct tgaacaaata gagtttcgca
    60721 agtattatgc ttacaaatta cttgagcaat taactaaaat ataaaccctg cctttatatc
    60781 taggcagggt ttatatttta gaaattcacg taggttgtta cggtttttac atacccagta
    60841 tagtttgagt ttctatagta ttcagtgata aacttccatt ttctttgagc aacatggata
    60901 taagtacttg ttatgtagta tggatatggg ctttgtgaat ccaagtaaga ctgataagct
    60961 tgtataccaa aatatgctcc accaattatt gcaccccatg gaccccccaa taaagcacct
    61021 atcctaccaa tcatataact gattccagca ccagtcatga agttagcgaa tgtgttagct
    61081 tgtttattcc catgtattgt gttgacgtaa ttccaaacat taggatcgta tgatctaaaa
    61141 gatatattta ggtcgatttc attcttttga taagccatat aaaatgcccc attgatatag
    61201 acgccgtcag cacgtcgttc aatagtgtct acacttccat ctggattgac aacctcaaga
    61261 acttcatcgc ttaaaatatt tacttgcgta tctccgaacc gcactgatga gccattctca
    61321 aactgagcct caccagatac aactttagag tttgccgata agctatcatc agcaaaaaca
    61381 acaagcgacg gggaaatgct agacatacag aaaacagaca taactagcaa acacatgcat
    61441 ttaaacatct tagacataac ggaaactcct ttgtattttt gatttttttc aacttttatt
    61501 atacaataaa accaaataaa aagaaagcgg taacaatatg cttaatgcga aaatttttta
    61561 tatattttta tgtttgatcg ttatcgaaac tacaggcttg ttgttgttga aaagaggtct
    61621 cgaaatgggt tatttagaca cagaagctat tatcctcgca gttttttcat ttgcttttta
    61681 caacctatgt tcattcgctt gggtctgctc tacaataaaa aacaataaaa aataaataga
    61741 cgtattttca aaaaaaaaaa aaaatgcata tttatcttag caaaacgacg atttaaatcg
    61801 tcgttttttt gt
//
LOCUS       DAVKLX010000006        59044 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKLX010000006 DAVKLX010000000
VERSION     DAVKLX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 59044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.006"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            107..6541
                     /locus_tag="VW325_000416"
     CDS             107..6541
                     /locus_tag="VW325_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9733880.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDNRIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
     gene            7173..7649
                     /locus_tag="VW325_000417"
     CDS             7173..7649
                     /locus_tag="VW325_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9733881.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            7687..7992
                     /locus_tag="VW325_000418"
     CDS             7687..7992
                     /locus_tag="VW325_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9733882.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            8040..9515
                     /locus_tag="VW325_000419"
     CDS             8040..9515
                     /locus_tag="VW325_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9733883.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            9820..16521
                     /locus_tag="VW325_000420"
     CDS             9820..16521
                     /locus_tag="VW325_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU9733884.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKNLTVEWEEVPAEN
                     LIKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(16769..17625)
                     /locus_tag="VW325_000421"
                     /pseudo
     CDS             complement(16769..17625)
                     /locus_tag="VW325_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(17836..18195)
                     /locus_tag="VW325_000422"
     CDS             complement(17836..18195)
                     /locus_tag="VW325_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9733885.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            18287..19243
                     /locus_tag="VW325_000423"
                     /pseudo
     CDS             18287..19243
                     /locus_tag="VW325_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            19227..19784
                     /locus_tag="VW325_000424"
     CDS             19227..19784
                     /locus_tag="VW325_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9733886.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            19777..20031
                     /locus_tag="VW325_000425"
     CDS             19777..20031
                     /locus_tag="VW325_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733887.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            20043..22097
                     /locus_tag="VW325_000426"
     CDS             20043..22097
                     /locus_tag="VW325_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9733888.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            22276..23136
                     /locus_tag="VW325_000427"
     CDS             22276..23136
                     /locus_tag="VW325_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9733889.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            23347..24057
                     /gene="ccdA2"
                     /locus_tag="VW325_000428"
     CDS             23347..24057
                     /gene="ccdA2"
                     /locus_tag="VW325_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9733890.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            24077..24643
                     /locus_tag="VW325_000429"
     CDS             24077..24643
                     /locus_tag="VW325_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9733891.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            24654..25766
                     /gene="msrB"
                     /locus_tag="VW325_000430"
     CDS             24654..25766
                     /gene="msrB"
                     /locus_tag="VW325_000430"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9733892.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEIMLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            26034..26771
                     /locus_tag="VW325_000431"
     CDS             26034..26771
                     /locus_tag="VW325_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9733893.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            26768..28459
                     /locus_tag="VW325_000432"
     CDS             26768..28459
                     /locus_tag="VW325_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9733894.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            28575..29138
                     /locus_tag="VW325_000433"
     CDS             28575..29138
                     /locus_tag="VW325_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9733895.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            29160..34829
                     /locus_tag="VW325_000434"
     CDS             29160..34829
                     /locus_tag="VW325_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEU9733896.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIF
                     DLEYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYL
                     EGRDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADI
                     EVHRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPY
                     GAIDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRIS
                     EEEANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTK
                     FTPYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGS
                     QKRYKVTSTGKFSITNLPEYQVEDLNVVYTSEHIVHPLDSSLINNLVEELKKVELYTE
                     STYQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAV
                     AALRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRL
                     GSSGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDY
                     IQFEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSR
                     EKRPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGS
                     EWVDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSE
                     YGISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAF
                     ERKNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFK
                     KVEPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFET
                     SYIAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRD
                     EGHAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEE
                     LQEGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            34890..36611
                     /gene="pabB"
                     /locus_tag="VW325_000435"
     CDS             34890..36611
                     /gene="pabB"
                     /locus_tag="VW325_000435"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9733897.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            36731..38257
                     /locus_tag="VW325_000436"
     CDS             36731..38257
                     /locus_tag="VW325_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9733898.1"
                     /translation="MKSKIVLGASLAIATLSLVSLVEIEGLSPFLIENVSANTHSANK
                     VINHKVSIYLENADEGKGLTVNFSTDSVSPNLFDEFEKKSGITITTMLVNAKTGEVVE
                     KRLTPSVFLRSNDLTSGTISSFIFSEYPDGEYKYVVSKGDFIDPKTQFKHQYRGESPV
                     FRIRNRKYVELGTTDKKLDERRDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSV
                     NSNLLNSFGEKAKKVLIRSKLINVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDL
                     TDGEYKYVAYESQQYTDPQTTLTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPK
                     PDEKPKPSAPQQEKPKPTVQSGWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYV
                     QNAWVGNYYLKSGGYMAKSEWIYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAK
                     SEWVYDSNYKSYYYLTSEGSYARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK
                     "
     gene            38344..39263
                     /locus_tag="VW325_000437"
                     /pseudo
     CDS             38344..39263
                     /locus_tag="VW325_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            39398..40357
                     /locus_tag="VW325_000438"
     CDS             39398..40357
                     /locus_tag="VW325_000438"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9733899.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFELAKEEDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            40454..41293
                     /locus_tag="VW325_000439"
     CDS             40454..41293
                     /locus_tag="VW325_000439"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9733900.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(41341..41511)
                     /locus_tag="VW325_000440"
     CDS             complement(41341..41511)
                     /locus_tag="VW325_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9733901.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            41632..42516
                     /gene="miaA"
                     /locus_tag="VW325_000441"
     CDS             41632..42516
                     /gene="miaA"
                     /locus_tag="VW325_000441"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9733902.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            42509..43747
                     /gene="hflX"
                     /locus_tag="VW325_000442"
     CDS             42509..43747
                     /gene="hflX"
                     /locus_tag="VW325_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9733903.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            43740..44363
                     /locus_tag="VW325_000443"
     CDS             43740..44363
                     /locus_tag="VW325_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9733904.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            44378..45307
                     /locus_tag="VW325_000444"
     CDS             44378..45307
                     /locus_tag="VW325_000444"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9733905.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            45328..46083
                     /locus_tag="VW325_000445"
     CDS             45328..46083
                     /locus_tag="VW325_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9733906.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(46137..47045)
                     /locus_tag="VW325_000446"
     CDS             complement(46137..47045)
                     /locus_tag="VW325_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9733907.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            47109..47378
                     /locus_tag="VW325_000447"
     CDS             47109..47378
                     /locus_tag="VW325_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9733908.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            47378..47758
                     /locus_tag="VW325_000448"
     CDS             47378..47758
                     /locus_tag="VW325_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9733909.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            47847..48074
                     /locus_tag="VW325_000449"
                     /pseudo
     CDS             47847..48074
                     /locus_tag="VW325_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(48114..48839)
                     /locus_tag="VW325_000450"
     CDS             complement(48114..48839)
                     /locus_tag="VW325_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU9733910.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            49001..50842
                     /gene="typA"
                     /locus_tag="VW325_000451"
     CDS             49001..50842
                     /gene="typA"
                     /locus_tag="VW325_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9733911.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            50862..51116
                     /locus_tag="VW325_000452"
     CDS             50862..51116
                     /locus_tag="VW325_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9733912.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            51480..51779
                     /locus_tag="VW325_000453"
                     /pseudo
     CDS             51480..51779
                     /locus_tag="VW325_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            51850..53871
                     /locus_tag="VW325_000454"
     CDS             51850..53871
                     /locus_tag="VW325_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9733913.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASFFILRSRDWVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            53873..54514
                     /locus_tag="VW325_000455"
     CDS             53873..54514
                     /locus_tag="VW325_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9733914.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLDLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWGQVDQVIRVTDLSHR"
     gene            54618..55970
                     /locus_tag="VW325_000456"
     CDS             54618..55970
                     /locus_tag="VW325_000456"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9733915.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            55972..57030
                     /locus_tag="VW325_000457"
     CDS             55972..57030
                     /locus_tag="VW325_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9733916.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQALFSKGG
                     FVSVPPVIAACVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            57040..58260
                     /gene="divIB"
                     /locus_tag="VW325_000458"
     CDS             57040..58260
                     /gene="divIB"
                     /locus_tag="VW325_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9733917.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYISVLFNDSEQIKAFT
                     SELAQINPELKVAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGVYSYTVADKLIMEAEEKAKKEAEEAEKRQEEERKRLEEEQKKQEE
                     ESNRNQTTQRSSRR"
BASE COUNT        18889 a        10379 c        12805 g        16971 t
ORIGIN      
        1 ctactctatt tcctagataa tacttagtaa aactttttat aacaaaggct agcaaagtta
       61 aagtttatta tctattggaa gttaaataaa tatgtaagga attaaaatga aaaaaagtac
      121 agtattgtca ttaaccacag ctgcagttat tttagcagcc tatgccccta atgaggtagt
      181 cttagcagac acatctagct ctgaagatgc tttaaacatc tctgataaag aaaaagtagt
      241 attagataag gaaacagaaa ataaagagaa acatgaagat attcataatg ctatagaaac
      301 ttcaaaggat actgaagaaa agaaaacaac agttattgag gaaaaagaag ttgttagtaa
      361 aaatcctgtg atagacacta aaactagcaa tgaagaagca agaatcaaag aagattccaa
      421 tcaatcccaa ggagataatg cacactcgtc tgcaaataaa ggcacagaaa atccgaaaaa
      481 agaagataga cttgtctata ttgctgaatt taaagataaa gaatctggag aaaaagcaat
      541 caagcaacta tccaatctta agaatacaaa agttttatat acttatgata gaatttttaa
      601 cggtagtgcc atagaaacaa ctccagataa cttggacaaa attaaacaaa tagaaggtat
      661 tacatcggtt gaaagggcac aaaaagtcca acccatgatg aatcatgcca gaaaggaaat
      721 tggagttgag gaagctattg attacctaaa gtctatcaat gctccgtttg ggaaaaattt
      781 tgatggtaga ggtatggtca tttcaaatat cgatactgga acagattata gacataaggc
      841 tatgagaatc gatgatgatg ccaaagcctc aatgagattt aaaaaagaag acttaaaagg
      901 aactgataaa aattattggt tgagtgataa aatccctcat gcgttcaatt attataatgg
      961 tggcaaaatc actgtagaaa aatatgatga tggaagggat tattttgacc cacatgggat
     1021 gcatattgca gggattcttg ctggaaatga tactgaacaa gatatcaaaa actttaacgg
     1081 catagatgga attgcaccta atgcacaaat tttctcttac aaaatgtatt ctgacgcagg
     1141 atctgggttt gcgggtgatg aaacaatgtt tcatgctatt gaagattcta tcaaacacaa
     1201 cgttgatgtt gtttcggtat catctggttt tacaggaaca ggtcttgtag gtgagaaata
     1261 ttggcaagct attcgggcat taagaaaagc aggcattcca atggttgtcg ctacgggtaa
     1321 ctatgcgact tctgcttcaa gttcttcatg ggatttagta gcaaataatc atctgaaaat
     1381 gaccgacact ggaaatgtaa cacgaactgc agcacatgag gatgcgatag cggtcgcttc
     1441 tgctaaaaat caaacagttg agtttgataa agttaacata ggtggagaaa gttttaaata
     1501 cagaaatata ggggcctttt tcgataagaa taaaatcaca acaaatgaag atggaacaaa
     1561 agctcctagt aaattaaaat ttgtatatat aggcaagggg caagaccaag atttgatagg
     1621 tttggatctt aggggcaaaa ttgcagtaat ggatagaatt tatacaaagg atttaaaaaa
     1681 tgcttttaaa aaagctatgg ataagggtgc acgcgccatt atggttgtaa atactgtaaa
     1741 ttactacaat agagataatt ggacagagct tccagctatg ggatatgaag cggatgaagg
     1801 tactaaaagt caagtgtttt caatttcagg agatgatggt gtaaagctat ggaacatgat
     1861 taatcctaat aaaaaaactg aagtcaaaag aaataataaa gaagatttta aagataaatt
     1921 ggagcaatac tatccaattg atatggaaag ttttaattcc aacaaaccga atgtaggtga
     1981 cgaaaaagag attgacttta agtttgcacc tgacacagac aaagaactct ataaagaaga
     2041 tatcatcgtt ccagcaggat ctacatcttg ggggccaaga atagatttac ttttaaaacc
     2101 cgatgtttca gcacctggta aaaatattaa atccacgctt aatgttatta atggcaaatc
     2161 aacttatggc tatatgtcag gaactagtat ggcgactcca atcgtggcag cttctactgt
     2221 tttgattaga ccgaaattaa aggaaatgct tgaaagacct gtattgaaaa atcttaaggg
     2281 agatgacaaa atagatctta caagtcttac aaaaattgcc ctacaaaata ctgcgcgacc
     2341 tatgatggat gcaacttctt ggaaagaaaa aagtcaatac tttgcatcac ctagacaaca
     2401 gggagcaggc ctaattaatg tggccaatgc tttgagaaat gaagttgtag caactttcaa
     2461 aaacacggat tctaaaggtt tggtaaactc atatggttcc atttctctta aagaaataaa
     2521 aggtgataaa aaatacttta caatcaagct tcacaataca tcaaacagac ctttgacttt
     2581 taaagtttca gcatcagcga taactacaga ttctctaact gacagattaa aacttgatga
     2641 aacatataaa gatgaaaaat ctccagatgg taagcaaatt gttccagaaa ttcacccaga
     2701 aaaagtcaaa ggagcaaata tcacatttga gcatgatact ttcactatag gcgcaaattc
     2761 tagctttgat ttgaatgcgg ttataaatgt tggagaggcc aaaaacaaaa ataaatttgt
     2821 agaatcattt attcattttg agtcagtgga agaaatggaa gctctaaact ccaacgggaa
     2881 gaaaataaac ttccaacctt ctttgtcgat gcctctaatg ggatttgctg ggaattggaa
     2941 ccacgaacca atccttgata aatgggcttg ggaagaaggg tcaagatcaa aaacactggg
     3001 aggttatgat gatgatggta aaccgaaaat tccaggaacc ttaaataagg gaattggtgg
     3061 agaacatggt atagataaat ttaatccagc aggagttata caaaatagaa aagataaaaa
     3121 tacaacatcc ctggatcaaa atccagaatt atttgctttc aataaccaag ggatcaacgc
     3181 tccatcatca agtggttcta agattgctaa catttatcct ttagattcaa atggaaatcc
     3241 tcaagatgct caacttgaaa gaggattaac accttctcca cttgtattaa gaagtgcaga
     3301 agaaggattg atttcaatag taaatacaaa taaagaggga gaaaatcaaa gagacttaaa
     3361 agtcatttcg agagaacact ttattagagg aattttaaat tctaaaagca atgatgcaaa
     3421 gggaatcaaa tcatctaaac taaaagtttg gggtgacttg aagtgggatg gactcatcta
     3481 taatcctaga ggtagagaag aaaatgcacc agaaagtaag gataatcaag atcctgctac
     3541 taagataaga ggtcaatttg aaccgattgc agaaggtcaa tatttctata aatttaaata
     3601 tagattaact aaagattacc catggcaggt ttcctatatt cctgtaaaaa ttgataacac
     3661 cgcccctaag attgtttcgg ttgatttttc aaatcctgaa aaaattaagt tgattacaaa
     3721 ggatacttat cataaggtaa aagatcagta taagaatgaa acgctattcg cgagagatca
     3781 aaaagaacat cctgaaaaat ttgacgagat tgcgaacgaa gtttggtatg ctggcgccgc
     3841 tcttgttaat gaagatggag aggttgaaaa aaatcttgaa gtaacttacg caggtgaggg
     3901 tcaaggaaga aatagaaaac ttgataaaga cggaaatacc atttatgaaa ttaaaggtgc
     3961 gggagattta aggggaaaaa ttattgaagt cattgcatta gatggttcta gcaatttcac
     4021 aaagattcat agaattaaat ttgctaatca ggctgatgaa aaggggatga tttcctatta
     4081 tctagtagat cctgatcaag attcatctaa atatcaaaag cttggcgaga ttgcagaatc
     4141 taaatttaaa aatttaggaa atggaaaaga gggtagtcta aaaaaagata caactggggt
     4201 agaacatcat catcaagaaa atgaagagtc tattaaagaa aaatctagtt ttactattga
     4261 tagaaatatt tcaacaatta gagactttga aaataaagac ttaaagaaac tcattaaaaa
     4321 gaaatttaga gaagttgatg attttacaag tgaaactggt aagagaatag aggaatacga
     4381 ttataaatac gatgataagg gaaatattat tgcttatgac gatggtagtg ccttacaata
     4441 tgaaactgaa aaacttgacg aaataaaatc aaaaatttat ggcgttctaa gcccatctaa
     4501 agatggacac tttgaaattc ttggaaagat aagcaatgtt tctaaaaatg ccaaggtata
     4561 ttatggaaat agctataaat cgatagaaat caaagcgact aagtatgact cacattcaaa
     4621 aacgatgaca tttgatttat acgctaatat taatgatatt gtggatggat tagcttttgc
     4681 tggagatatg cggttttttg ttaaagatga taatcgaata aaagctgaaa ctaaaattag
     4741 aatgcctgaa aaaaataagg aaactaaagc agaatatccc tatgtatcaa gttatgggaa
     4801 tgtaatcgaa ttaggagaag gagatctttc aaagaacaaa ccagacaatt taactaaaat
     4861 ggaatctggt aaaatctatt ctgattcaga aaaacaacaa tatctgttaa aggataacat
     4921 cattctaaga aaaggctatg cactaaaagt gactacctat aatcctggaa aaacggatat
     4981 gttagaagga aatggagtct atagcaagga agatatagca aaaatacaaa aggccaatcc
     5041 taatctaaga gtcctttcag aaacaacaat ttatgctgat agtagaaatg ttgaagatgg
     5101 aagaagtacc caagctgtat taatgtcggc tttggacggc tttaacatta taaggtatca
     5161 agtgtttaca tttaaaatga acgataaagg ggaagctatc gataaagacg gaaatcttgt
     5221 gacagattct tctaaacttg tattatttgg taaggatgat aaagaataca ctggagagga
     5281 taagtccaat gtagaagcta taaaagaaga tggctccatg ttatttattg ataccaaacc
     5341 agtaaacctt tcaatggata agaactactt taatccatct aaatctaata aaatttatgt
     5401 acgaaatcca gaattttatt taagaggtaa gatttctgat aagggtggtt ttaactggga
     5461 attgagagtt aatgaatcgg ttgtagataa ttatttaatc tacggagatt tacacattga
     5521 taacactaga gattttaata ttaagctgaa tgttaaagac ggtgacatca tggactgggg
     5581 aatgaaagac tataaagcaa acggatttcc agataaggta acagatatgg atggaaatgt
     5641 ttatcttcaa actggctata gcgatttgaa tgctaaagca gttggagtcc actatcagtt
     5701 tttatatgat aatgttaaac ccgaagtaaa cattgatcct aagggaaata ctagtatcga
     5761 atatgctgat ggaaaatctg tagtctttaa catcaatgat aaaagaaata atggattcga
     5821 tggtgagatt caagaacaac atatttatgt aaatggaaaa gaatatacat catttgatga
     5881 tattaaacaa ataacagaca agacactaaa cattaagatt gttgtaaaag attttgcaag
     5941 aaatacaacc gtaaaagaat tcattttaaa taaagatacg ggagaggtaa gtgaattaaa
     6001 acctcatagg gtaactgtga ccattcaaaa tggaaaagaa atgagttcaa cgatagtgtc
     6061 ggaagaagat tttattttac ctgtttataa gggtgaatta gaaaaaggat accaatttga
     6121 tggttgggaa atttctggtt tcgaaggtaa aaaagacgct ggctatgtta ttaatctatc
     6181 aaaagatacc tttataaaac ctgtattcaa gaaaatagag gagaaaaagg aggaagaaaa
     6241 taaacctact tttgatgtat cgaaaaagaa agataaccca caagtaaacc atagtcaatt
     6301 aaatgaaagt cacagaaaag aggatttaca aagagaagat cattcacaaa aatctgattc
     6361 aactaaggat gttacagcta cagttcttga taaaaacaat atcagtagta aatcaactac
     6421 taacaatcct aataagttgc caaaaactgg aacagcaagc ggagcccaga cactattagc
     6481 tgccggaata atgtttatag taggaatttt tcttggattg aagaaaaaaa atcaagatta
     6541 agacaaaagc tatagaaaaa aatggtttat gtactgagat tagatagtga ggtgatgaca
     6601 tagttttgtg aaaacggcca tttataactc aattatttag tttacttcac ttcactattg
     6661 atactatttg gagttattaa tggacttagt ttagataact aatgaattga ttgaaagggt
     6721 tagtattgac aatattggtc atattgatta gaaaatagag tctatcaaaa tttaaaggct
     6781 aatagaggtg atgagacaat ttcaaggaat taagtagcgt ttggttgctt aattcctttt
     6841 tatttggaag gaagtttttc gtaaccgccc aataacgaag tctattggac ggttacgata
     6901 tgatcactga cttcgtcagt cttatctaca acctcaaaac tgtgttttga gcaacctgcg
     6961 gctagcttcc tagtttactc tttgattttc attgagtatt agaacagaaa aaatgcttgg
     7021 agtatttgtt tgtgtgttta tttttatata acaaactata aacaaaacaa aaatataaaa
     7081 aaagagacaa aaaagaacaa aaagtaattg acaacggttt catatagtgt tatacttatg
     7141 gcataaagtt aatggaaaga agggaaaaac ttatggtatt agattatttc tttgacaaaa
     7201 accttgtgtt ttgcttagaa gcggataatc aagaacatct ctttgatcag gttgcaactt
     7261 tattggaaga acgagaaatt gtaactccaa cttatcgtga agccttgatc acgcgtgaaa
     7321 agtcatttcc aactggttta gatatggaat ttctaggaaa ggacttgcca aatgtagcga
     7381 ttcctcacac agatattgtt cataatctag ctgagaaagt ggtggttgtt cgattagaaa
     7441 aaccagtaac ttttcacaat atgatagctc ctgataagga agtagaagta tctctactct
     7501 tctttatcat taacaattca agttcaagtc aaacgaacat tctggctcag ttgatggact
     7561 ttttcacagg aaatggtcat cttgaagacc tatcaaaaat ttctgaacca gaaaaacttt
     7621 atgcttacat tgctgaagca accgcttaat cttgtctatt aaaaaataaa atcggaggaa
     7681 atccaaatga ttaaaattct tgctgcctgc ggtgcaggtg ttaactcaag tcaccaaatt
     7741 aaaagtgctc tagaagaaga actttcaaac cgtggttatg atgttcactg tgatgcagtc
     7801 atggtgaaag atgtaaacga agaccttatg aaaggttacg atatctttac accaatcgct
     7861 gcaacagacc ttggttttga accaggtatc ccagttatcg aagctgggcc aatcttattc
     7921 cgtatcccag caatgagcgc tccagtattt gacaatattg aagcagctat taaagaacat
     7981 ggattaagct aattctaatt tgttaacatt catataacat aaaaaaggga ggaactgtta
     8041 tggatgcaat ctttgaccta atcggaaagg ttttcaatcc catcttagaa atgggtggac
     8101 ctgtcatcat gttgatcatt ttgacagtat tggctttact ttttggagtg aaattctcca
     8161 aagcgcttga aggtggtatc aaacttgcca tcgctcttac aggtatcggt gctatcatcg
     8221 gtatgctaaa tggtgctttc tcagcatcac ttgcaaaatt cgttgaaaac actggtatcc
     8281 aattgagtat tacagacgtt ggttgggcac cactcgcaac aatcacttgg ggttctgctt
     8341 ggacactata cttcttgctc atcatgttga ttgtcaacgt agtgatgctt gctatgaaga
     8401 aaaccgatac acttgatgtc gatatctttg atatctggca cttgtctatc acaggtctct
     8461 tgattaaatg gtatgctgat aacaatggtg tgagtcaagg ggtttcactc tttattgcta
     8521 cagcagctat cgtccttgtc ggtgtgttga aaattatcaa ctctgacttg atgaaaccta
     8581 catttgatga ccttcttaac gccccaagtt catcaccaat gacatcaact cacatgaact
     8641 acatgatgaa cccagttatc atggttttgg ataagatttt tgaaaaattc ttcccaggcc
     8701 ttgataaata tgactttgat gctgctaaat tgaacaagaa aatcggtttc tggggatcta
     8761 aattcttcat cggtttcatc cttggtatcg ttatcggtat tatgggaact ccacatccaa
     8821 ttgcaggtgt tgcagatgca gataaatggc gtcttgttat caaaggatgg ttgtctcttg
     8881 gtttgactgc cggtgtatct ttggaactct tctcacttat cggttcatgg ttcatcgcag
     8941 ccgtagaacc actatcacaa ggtattacaa acgttgctac taaacgtctt cgaggacgta
     9001 aattcaatat cggtcttgac tggccattca tcgctggtcg tgctgaaatc tgggcttgtg
     9061 ccaacgtact tgcaccaatc atgttgattg aagcagtgct tctttcaaaa gttggaaatg
     9121 gtatcttgcc acttgcaggt atcatcgcta tgggtgttac tccagctctc ttggttgtaa
     9181 ctcgtggtaa attgctccgt atgattatct tcggaacact cttgttgcca ctcttccttc
     9241 tttcaggtac acttattgca ccatttgcaa cagaacttgc taaaggtgta ggtgccttcc
     9301 cagaaggtgt gagccaaact caattgatta ctcactctac tcttgaagga ccaatcgaaa
     9361 aacttcttgg ttggacaatt ggtaacacta caactggtga tatcaaagca atccttggtg
     9421 cagtagtctt ccttgtattc tatatcggta tctttgcttg gtacagaaaa caaatgatca
     9481 aacgtaacga agagtacgca gcaaaagcaa aataatgcgc tcctataaaa tataaactca
     9541 atgaaaatca aagagcaaac taggaaacta gccgcaggtt gctaaaaaca cagttttgag
     9601 gttgtagata agactgacga agtcagctca aaacatggtt ttgaggttgt agatgaaact
     9661 gacgaagtca gtaatcatat ctacggcaag gcgacgctga cgaagtttga attggatttt
     9721 cgcagagtgt aaattgttga aactaggttg tcataccatg tataccactt gggctttggt
     9781 agtagctaac tgcactaaat ataatataag gagggtttta tggggaaagg ccattggaat
     9841 cggaaaagag tttatagcat tcgtaagttt gctgtgggag cttgctcagt aatgattggg
     9901 acttgtgcag ttctattagg aggaaatata gctggagaat ctgtagttta tgcggatgaa
     9961 acacttatta ctcatactgc tgagaaacct aaagaggaaa aaatgatagt agaagaaaag
    10021 gctgataaag ctttggaaac taaaaatgta gttgaaagga cagaacaaag tgaacctagt
    10081 tcaactgagg ctattgcatc tgagaagaaa gaagatgaag ccgtaactcc aaaagaggaa
    10141 aaagtgtctg ctaaaccgga agaaaaagct ccaaggatag aatcacaagc ttcaagtcaa
    10201 gaaaaaccgc tcaaggaaga tgctaaagct gtaacaaatg aagaaatgaa tcaaatgatt
    10261 gaagacagga aagtggattt taatcaaaat tggcacttta aactcaatgc aaattctaag
    10321 gaagccatta aacctgatgc agacgtatct acgtggaaaa aattagattt accgtatgac
    10381 tggagtatct ttaacaattt cgatcatgaa tctcctgcac aaaatgaagg tggacaactc
    10441 aacggtgggg aagcttggta tcgcaagact ttcaaactag atgaaaaaga cctcaagaaa
    10501 aatgttcgcc ttacttttga tggcgtctac atggattctc aagtttatgt caatggtcag
    10561 ttagtggggc attatccaaa tggttataac cagttctcat atgatatcac caaatacctt
    10621 cacaaagatg gtcgtgagaa tgtgattgct gtccatgcag tcaacaaaca gccaagtagc
    10681 cgttggtatt caggaagtgg tatctatcgt gatgtgactt tacaagtgac agataaggtg
    10741 catgttgaga aaaatgggac aactatttta acaccaaaac ttgaagaaca acaacatggc
    10801 aaggttgaaa ctcatgtgac cagcaaaatc gtcaatacgg acgacaaaga ccatgaactt
    10861 gtagccgaat atcaaatcgt tgaacgaggt ggtcatgctg taacaggctt agttcgtaca
    10921 gcgagtcgta ccttaaaagc acatgaatca acaagcctag atgcgatttt agaagttgaa
    10981 agaccaaaac tctggaccgt tttaaatgac aaacctgcct tgtacgaatt gattacgcgt
    11041 gtttaccgtg acggtcaatt ggttgatgct aagaaggatt tgtttggtta ccgttactat
    11101 cactggactc caaatgaagg tttctctttg aatggtgaac gtattaaatt ccatggagta
    11161 tccttgcacc acgaccatgg ggcgcttgga gcagaagaaa actataaagc agaatatcgc
    11221 cgtctcaaac aaatgaagga gatgggagtt aactctatcc gtacaaccca caaccctgct
    11281 agtgagcaaa ccttgcaaat cgcagcagaa ctaggtttac tcgttcagga agaggccttt
    11341 gatacgtggt atggtggcaa gaaaccttat gactatggac gtttctttga aaaagatgcc
    11401 actcacccag aagctcgaaa aggtgaaaaa tggtctgatt ttgacctacg taccatggtc
    11461 gaaagaggca aaaacaaccc tgctatcttc atgtggtcaa ttggtaatga aataggtgaa
    11521 gctaatggtg atgcccactc tttagcaact gttaaacgtt tggtcaaggt tatcaaggat
    11581 gttgataaga ctcgctatgt taccatggga gcagataaat tccgtttcgg caatggtagc
    11641 ggagggcatg agaaaattgc tgatgaactc gatgctgttg gatttaacta ttctgaagat
    11701 aattacaaag cccttagagc taagcatcca aaatggttga tttacggttc agaaacatca
    11761 tcagcaaccc gtacacgagg aagttactat cgccctgaac gtgaattgaa acatagcaat
    11821 ggacctgagc gtaattatga acagtcagat tatggaaatg atcgtgtggg ttgggggaaa
    11881 acagcaaccg cttcatggac ttttgaccgt gacaacgctg gctatgctgg acagtttatc
    11941 tggacaggta cggactatat tggtgaacct acaccatggc acaaccaaaa tcaaactcct
    12001 gttaagagct cttactttgg tatcgtagat acagccggca ttccaaaaca tgacttctat
    12061 ctctaccaaa gccaatgggt ttctgttaag aagaaaccga tggtacacct tcttcctcac
    12121 tggaactggg aaaacaaaga attagcatcc aaagtagctg actcagaagg taagattcca
    12181 gttcgtgctt attcgaatgc ttctagtgta gaattgttct tgaatggaaa atctcttggt
    12241 cttaagactt tcaataaaaa acaaaccagc gatgggcgga cttaccaaga aggtgcaaat
    12301 gctaatgaac tttatcttga atggaaagtt gcctatcaac caggtacctt ggaagcaatt
    12361 gctcgtgatg aatctggcaa ggaaattgct cgagataaga ttacgactgc tggtaagcca
    12421 gcggcagttc gtcttattaa ggaagaccat gcgattgcag cagatggaaa agacttgact
    12481 tacatctact atgaaattgt tgacagccag gggaatgtgg ttccaactgc taataatctg
    12541 gttcgcttcc aattgcatgg ccaaggtcaa ctggtcggtg tagataacgg agaacaagcc
    12601 agccgtgaac gctataaggc gcaagcagat ggttcttgga ttcgtaaagc atttaatggt
    12661 aaaggtgttg ccattgtcaa atcaactgaa caagcaggga aattcaccct tactgctcac
    12721 tctgatctct tgaaatctag tcaagtcact gtctttactg gtaagaaaga aggacaagaa
    12781 aagactgttt tggggacaga ggtgccaaaa gtacagacca ttattggaga ggcacctgaa
    12841 atgcctacca ctgttccgtt tctatacagt gatggtagtc gtgcagaacg tcctgtaacc
    12901 tggtcttcag tagatgtgag caagcctggt attgtaacgg tgaaaggtat ggctgacgga
    12961 cgagaagtag aagctcgtgt agaagtgatt gctcttaaat cagagctacc agttgtgaaa
    13021 cgtattgctc caaatactga cttgaattct gtagacaaat ctgtttccta tgttttgact
    13081 gatggaagtg tacaagagta cgaagtagat agctgggaga ttacggaagt agataaagcc
    13141 aaactttcag tagctggatc acgtattcaa atgactggtc agttagctgg agaaactatt
    13201 catgcaaccc ttgtggtaga agaaggaaat gctgcagcac ctgtagtgcc aactgttact
    13261 gttggaggtg aagcagtaac aggtcttact agtcgacaac caatgcaata tcgtactcta
    13321 tcttatggtg cccaattgcc agaagtcaca gcaagtgctg aaaatgctga tgtgacagtt
    13381 cttcaagcaa gcgcagcaaa cggcatgcgt gcaagcatat ttgttcagcc taaagatggt
    13441 ggccctcttc aaacctatgc aattcaattc ctagaagaag caccaaaaat tgctcacttg
    13501 agcttgcaag tggaaaaagc tgacagtctc aaagaagacc aaactgtcaa attgtcggtt
    13561 cgagctcact atcaagatgg aacgcaagct gtattaccag ctgataaagt aaccttctct
    13621 acaagtggtg aaggggaagt cgcaattcgt aaaggaatgc ttgagttgca taagccagga
    13681 gcagtcactc tcaaagcgga atatgaggga gctaaaggcc aagttgaact cactatccaa
    13741 gccaatactg agaagaagat tgcgcaatct atccgtcctg taaatgtagt cacagatttg
    13801 catcaaaaac ctactcttcc aacaacagta acggttgagt atgacaaagg tttccctaaa
    13861 gctcacaaag tcacttggca agctattccg aaagaaaaac tagactccta tcaaacattt
    13921 gaagtactag gtaaagttga aggaattgac cttgaagcgc gtgcaaaagt ctctgtagaa
    13981 ggtatcgttt cagttgaaga agtcagtgtg acaactccaa tcgcagaagc accacaatta
    14041 ccagaaagtg ttcggacata tgattcaaat ggtcacgttt catcagctaa ggttgcatgg
    14101 gatgcgattc gtccagagca atacgctaag gaaggtgtct ttacagttaa tggtcgctta
    14161 gaaggtactc aattaacaac taaacttcat gttcgcgtat ctgctcaaac tgagcaaggt
    14221 gcaaacattt ctgaccaatg gaccggttca gaattgccac ttgcctttgc ttcagactca
    14281 aatccaagcg acccagtttc aaatgttaat gacaagctca tttcctacaa taaccaacca
    14341 gccaatcgtt ggacaaactg gaatcgtact aatccagaag cttcagtcgg tgtcctattc
    14401 ggagattcag gtattttgag caaacgttca gttgataact taagcgttgg cttccatgaa
    14461 gaccatggag ttggtgcacc gaagtcttat gtgattgagt attatgttgg taagactgtc
    14521 ccaacagctc ctaaaaaccc tagttttgtt ggtaatgagg accatgtctt taatgattct
    14581 gccaactgga aaccagttac taatctaaaa gcccctgctc aactcaaggc tggagaaatg
    14641 aaccacttta gctttgataa agttgaaacc tatgctgttc gtattcgcat ggtgagagca
    14701 gacaacaaac taggaacgtc tatcacagag gtacaaatct ttgcgaaaca agttgcggca
    14761 gccaagcaag gacaaacaag aatccaagtt gacggtaaag acttagcaaa cttcaaccct
    14821 gatttgacag actactacct tgagtctgta gatggaaaag ttacggcagt cacagcaagt
    14881 gttagcaaca atggcctcgc taccgtcgtt ccaagcgttc gtgaaggtga gccggttcgt
    14941 gtcatcgcga aagctgaaaa tggcgacatc ttaggagaat accgtctgca cttcactaag
    15001 gataagaact tactttctca taaaccagtt gctgcggtta aacaagctcg cttgctacaa
    15061 gtaggtcaag cacttgaatt gccgactaag gttccagttt acttcacagg taaagacggc
    15121 tacgaaacaa aaaacctgac agttgaatgg gaagaagttc cagcggaaaa tctgataaaa
    15181 gcaggtcaat ttaccgttcg aggccatgtc cttggtagtg atcttgttgc tgaggtcact
    15241 gtacgagtga cagacaaact aggcgaagct ctttcagata accctaacta tgatgaaaac
    15301 agtaaccagg cctttgcttc agcaaccaat gatattgaca aaaactctca tgaccgcgtt
    15361 gactatctca atgacggaga tcattcagaa aatcgtcgtt ggacaaactg gtctccaaca
    15421 ccatcttcta atccagaagt atcagcgggt gtgatcttcc gtgaaaatgg taagattgta
    15481 gaacggactg ttgctcaagc caaacttcac ttctttgcag atagtggtac ggatgcacca
    15541 actaaactcg ttttagaacg ctatgtcggt ccagagtttg aagtgccaac ctactattca
    15601 aactaccaag cctacgacgc agaccatcca ttcaacaatc cagaaaattg ggaagctgtg
    15661 ccttatcgtg cggataaaga catcgcagct ggtgatgaaa tcaacgtaac atttaaagct
    15721 gtcaaagcca aagctatgag atggcgtatg gagcgtaaag cagataagag cggtgttgcg
    15781 atgattgaga tgaccttcct tgcaccaagt gaattgcctc aagaaagcac gcaatcgaag
    15841 attcttgtag atggaaaaga acttgctgat ttcgctgaaa atcgtcaaga ctatcaaatt
    15901 acctataaag gtcaacggcc aaaagtctca gttgaagaaa acaatcaagt agcttcaact
    15961 gtggtagata gtggagaaga tagccttcca gtacttgttc gcctcgtttc agaaagtgga
    16021 aaacaagtca aggaataccg tatccagttg actaaggaaa aaccagtttc tgagaagaca
    16081 gttgctgctg tacaagaaga tcttccaaaa ctcgaatttg ttgaaaaaga tttggcctac
    16141 aagacagttg agaaaaaaga ttcaacactg tatctaggtg aaactcgtgt agaacaagaa
    16201 ggaaaaactg gtaaagaacg tatctttaca gcgattaatc ctgatggaag taaggaagaa
    16261 aaactccgtg aagtggtaga agctccgaca gaccgcatcg tcttggttgg aaccaaacca
    16321 gtagctcaag aagctaaaaa accacaagtg tcagaaaaag cagatacaaa accaattgat
    16381 tcaagtgaag ctagtcaaac taataaagcc cagttaccaa atacaggtag tgcggcaagc
    16441 caagcagcag tagcagcagg tttagctctt ctaggtttga gtgcaggatt agtagttact
    16501 aaaggtaaaa aagaagacta gaaattttga taccttcttt atcattaaag accaaagaaa
    16561 aactcttgtc ctaaatagaa gataaagagt ctggaacaaa atagttctca accacaaaaa
    16621 agctagatat ttccaattgc ggaactctag ctttttaatt ttgagtcgtt ctcttattgt
    16681 atagtagatt gaaactagag tagtacactt ctacttctaa aacattgtta gaaatcgatt
    16741 tgactgtcct tatcgatttg tcctgttctt atttcatttc actatgtttt aggaggttat
    16801 tggccataag taccaatccc atgtcaattc tcacttgacg cttgcctctc agattacatc
    16861 tcttgtaacc caaacaagcc tttatctgcc caaagacagg ttctacatca atcttgcgtt
    16921 gagcgaaaat ctgtctatct tggggagata aaagcgcctg acattcttta gctttcaagt
    16981 tttgatagcg ttcgttcata tacaatccct tttgaggggc tgattcaggt tcatcagcgt
    17041 agtaaacctt gatttcctgt tgaaagtctg tctgtgtttt ctggtacttg atatggtgaa
    17101 aacgatagca ccagccatca ggatgtatgt agctatcctc cttgtcatta tagtgccaat
    17161 tcgctaagtt tttagctgac tgtttatacc ctctcttttg ttccttatca aacatggcat
    17221 atttaatcag aaggggagtg atttcttctt gaaaatagac ttgtatctgt tcttgaagtt
    17281 tggcggagaa tttctctgtc gttttcttcc acacaaaact atacttgttg gcattggatt
    17341 caatcttagt tccgtcaata aacaaacaat ctaaggtcac taactcttcc atttttcaat
    17401 ttttcgacca gagaaaatcc cttgtgaata ggcaaataga agagtagata caagcatttt
    17461 agggtgataa gacgggcgac caaaggcatg ataggaggtg tagaagtgac gttcctccaa
    17521 ggtattcacc actttttcaa tagtaaagac gagatggtct tgtggtaaga aggaactgat
    17581 ttctagtggt aaagttgttt gatttgtgtt atagtgaata tgcatgggat agcctttcta
    17641 aatggtttat taagcaattc tattttacca agactatcgt aaccatgcaa aaaagcaacg
    17701 gcaaatccgt tgcctttttt ggatataaga gactattgtc ccagactctt ttctttgtat
    17761 aaaattgtat agtgttattg tctatactat tttgtagaga aagcaaaaat ccctagaaaa
    17821 tgcttaggga ttcgactatt tcattaagaa caattcaaca atcatctcta tatacatgat
    17881 aagggttaat aaaaatcctg tacgccagaa gaatttgatg aatttaggat agtaaaagct
    17941 gcgttttttt aaaaggaaga aaaagacgag aataatggct aggaacgaga gggctagccc
    18001 cagtctaggg aggaaattat gggtaaaggt tttagctgta atcaagtaat actcaaatac
    18061 caagactgga aaagccaaat ccgcaaagtt tcgtcctagt ttttttaatc taaaaagttt
    18121 ggttaggata atgcagacga ctaaggtcag tatcaataat aaaatagatg ctaatttcat
    18181 taaaatcata cccatattgt atcataaaaa aaggctaatg gaaacgagaa acagaggaat
    18241 ttataggaaa gtcaggcttt tgagattgtg ggtgattttt gttataatga aagttataaa
    18301 atcttatgat accttgaatg attattatcg aaaactcttt ggagaaaaga cttttaaagt
    18361 ccctattgat gcgggatttg actgtcccaa tcgtgatgga actgtggctc atggaggctg
    18421 tactttttgt acggtttccg gttctggaga tgccattgta gccccagatc cgcctatccg
    18481 tgagcaattt tataaggaaa ttgactttat tcatcgtaag tggccagatg ttcagaagta
    18541 tctggtttat tttcaaaatt ttaccaacac ccatgaaaag gtggaagtta ttcgagagcg
    18601 ttatgaacag gctatcaatg agccaggtgt ggtaggaatc aatattggaa cgcgcccaga
    18661 ctgtttacca gacgaaacca tcgaatattt ggctgagtta tcggagtgca tgcatgtgac
    18721 ggttgaattg ggcttgcaga ctacttatga agcaacctct gacctgatta accgtgcgca
    18781 ttcttatgaa ttgtaggtgg aaacggtcaa gcgtttgaga aagtatccaa aaattgagat
    18841 tgtttcccat ttgatcaatg gtttgcctgg tgaaacccat gagatgatgg ttgaaaatgt
    18901 ccgccgttgt gtcacggata acgatattca agggattaaa ctgcatttgc tccatcttat
    18961 gacaaatact cgtatgcaac gagattacca tgagggacgt ttacaattga tgagtcagga
    19021 cgaatatgtc agggtcatct gtgaccaact ggaaattatt cccaaacata tcgtcatcca
    19081 tcgaattaca ggagatgcgc ctagggatat gctgattggt cctatgtgga gcctcaataa
    19141 atgggaagtc ctcaacagca ttgagatgga gatgagacgt cgtggaagtg ttcaaggatg
    19201 caaggctgta aaacaggagt ttgaaaatga aaagaccact tgagatggca catgattttt
    19261 tggctgaggt cgtgacaaaa gaggatgtcg tagtggatgc gactatggga aatggtcatg
    19321 acacgctttt tttagccaag ctagccaagc aagtctatgc ctttgatatt cagaagcaag
    19381 ccttggaaaa gacccaagag cgtttgcatc aggctggctt gacaaatgcc cagttaatct
    19441 tgcaaggcca tgagacactg gaccagtttg tgacaaaagc taaggcaggg atttttaatt
    19501 tgggctattt gccgtcagct gataagtctg tcatcacccg accgcagaca acgattgagg
    19561 cattagaaaa gctatgtggc ttacttgtca aaggtggacg aattgctatt atgatttact
    19621 atggtcatga aggaggcgac ctcgagagag atgctgtctt ggattttgtg atccagttga
    19681 accaacaaga gtacacagct gccatttacc gaactttaaa ccaagtcaac aacccgccgt
    19741 ttttagtgat gattgaaaaa ttagagagat acagacatgg ataaacaata cctacacgaa
    19801 aagctggatg ccatgcgcca gaattttgtt gaatcaacct accacgaacg agcggtgggc
    19861 gtgctagacc aagcgcatat gagcaaaaaa atgcttaaaa tcaagaaaaa attagttgct
    19921 cttgaaatgg aacggtgcca gagaaaaatt gagcacaaag actgttccaa gattgaccaa
    19981 aaaatcaaag agcagaagga gatatttgaa tcctgttgta aaaaagatta aggaggacgt
    20041 gcgtggaatt actgatttac ctcatcctat ttttactggt cttgattgtc tcaagtacaa
    20101 ccaataagct cctacccttt ttgcctctcc ctttggtgca gattcttttg ggaattgtga
    20161 ttggtctctt tttacccaat actgactttc accttaatac ggagttgttt ttggcactgg
    20221 ttatcggccc cttgcttttc cgagaggctg aagaagcaga tgttacggct attttaaaac
    20281 actggcgaat cattgtttat ctcatatttc cagtgatttt tatctcgacc ctgagtttgg
    20341 gtggcttggc ccatcttctt tggttcagcc ttcccttggc agcttgcttg gctgttgggg
    20401 cagcccttgg tcctacggac ttggtggcct ttgcctctct ttcggagcgt tttagctttc
    20461 ctaagcgcgt gtccaatatt cttaagggcg aaggactctt gaatgatgct tctggtttgg
    20521 tggcttttca ggtagctttg acagcttgga caactggagc tttttctctg gggcaagcta
    20581 gcagttcact catcttttca atcctaggcg gttttttaat tggattttta acagctatga
    20641 ccaaccgctt cctccatacc ttcttgctaa gtgtgcgcgc aacggatatt gccagtgaac
    20701 ttttattaga gttgagtttg cctctagtga ccttctttct ggcagaagaa gtccatgttt
    20761 cagggattat tgccgtcgta gttgctggaa ttttaaaggc aagtcgcttc aagaaaatca
    20821 cgctcctcga agcccaagtg gatacggtga ccgagacggt ctggcataca gtgaccttta
    20881 tgctcaacgg ttctgtcttt gtgattttag ggatggagtt ggaaatgata gcagaaccta
    20941 tcttgaccaa tccaatctat aatcctctac ttttattgct atctctcatc gcccttacct
    21001 ttgtcctctt tgtcattcgt tttattatga tctatggcta ttatgcctat agaacccgac
    21061 gcctcaagaa aaagctaaat aagtatatga aggacatgtt tctcttgacc ttttcaggtg
    21121 ttaagggaac ggtgtcgatt gctacgattc tcttgatacc aagtaatcta gaacaggagt
    21181 atcctctctt gcttttcctt gttgcaggtg tgacgcttgt cagcttttta acaggtctct
    21241 tggtcttgcc tcatctttct gatgaagagg aagaaagcaa ggattatctc atgcatatcg
    21301 ccattttgaa tgaagtaacg ctagagttgg aaaaagagtt ggaagacacc agaaataaac
    21361 ttcccctcta tgcggctatt gacaattatc atggacgtat tgaaaatctc attttaagcc
    21421 aagaaaacca ggatgatcaa gaagactggg ctgctttgaa actcttgatt cttagtattg
    21481 aaagtgatgg tttggaacag gcctatgaag aggggaacat tagcaatcgt gcttaccgag
    21541 tttaccaacg ttatctgaaa aatatagaac aaggaatcaa tcgtaaactt gcctcaagac
    21601 tgacctatta ttttcttgtt tccttgagga ttttacgttt tctccttcat gaagttttta
    21661 ctcttggaaa gaccttccgt agctggaagg acaaggagca aagccgtctc cgtgctcttg
    21721 attatgacca aattgcagag ctctatcttg ccaatacaga gatgattatt gaaagtttgg
    21781 aaaacctgaa gggagtctac agacgctctt tgattagttt tatgcaggag tctcgtcttc
    21841 gagaaacagc tattatcagc agtggtgcct ttgtcgaacg ggttatcaat cgtgtcaaac
    21901 ccaacaatat cgatgaaatg ctgagaggct attatctgga gcgcaagttg attttcgaat
    21961 acgaagaaaa acgattgatt acgactaagt atgccaagaa attacgacaa aatgtaaata
    22021 acttagagaa ctattccttg aaggaagctg ccaataccct accgtatgat atggtggaat
    22081 tggtaagaag aaattagtta atactcttcg aaaatctctt caaaccacgt cagcgtcgcc
    22141 ttaccgtatg tatgtgactg acttcgtcag tcttatctac aacctcaaaa cagtgttttg
    22201 agcagcttgc ggctagcttt ctagtttgct ctttgatttt cattgagtat aagattataa
    22261 gtgaaggagt atgacatgaa aaagtggtgg aaagagctga tagacaaacc tttattaaaa
    22321 gcttttttgc attattatca agcatcagat agtgagttga ccagtgtcgc agtagcctac
    22381 tattggttga tttcgatttt ccccctgctt ttggtggtgg tcaatatcct cccctatttt
    22441 cagattcctg tgggagaatt tctgggcttt atgaaagacg tcctgccccc aagcctctat
    22501 gaaggtgtgg aaaaaatagc cagagaagtc ttgacgcaac cttcaacagg tttattgagt
    22561 ttttctgttt tatcggcgtt atggagtttt tcaaaatcta tgaatttctt gcaaaaagct
    22621 tttaacaagg cttatggcgt cgaaaagagt cgtggactta tttcccatca gatgctaagt
    22681 ctcctagtca gttttggctt gcagctcctc tttgcgtttg ccttgttttt gatcttattt
    22741 ggtcagatga ttttggcttt acttgctcat tattggacaa aagatggcat tatctatcag
    22801 gctttacaag ggttggcagg tcctctgatt tacgccctcc tctttgctat cttggtcatg
    22861 ctttattatt ttcttcccaa tctatctaac aggaaaatta gctataccct accaggcagt
    22921 gcctttgttc tcttagtgat tttaggttta ctaactctgt tctctagtta tctcaattac
    22981 tatgtccatc atttggtgga tgtccgaatt ttaggttccg ttcttcttgt tgttatgatg
    23041 ttttggttta tcttgattgc taaaattgtt atcctaggcg cggttatcaa tgccagtatg
    23101 cagagtttga aagatccggt ctttaaaaaa gactaactat ttatccatta taaaagcgca
    23161 tagtatcaag actttataga accttgatac tatgcgcttt tgtgctttga cagttttttg
    23221 tatagttttt gtccaagatt tctaaatcat ctgattaaat tcctattttc ccctatcatt
    23281 gtccctgttt tttcgaaaat ggaaggagta taataaatct atcaataaaa tgataggagg
    23341 aactttatgg gtcatatttt cttttttcta agtgtctttt tggcagggat tctatccttc
    23401 ttttctcctt gtatcttacc tttgttaccg gtctatacag gagtgttact agatgataag
    23461 gatggtgctc aggcttctag cggcaaattt tcaatctcag ttactagttt attacgaact
    23521 ctggccttta tagcaggaat ttcctttata tttattttgt tgggctatgg agctggtttt
    23581 ttaggcgatt tgctttatgc ttcttggttc caatatctta ctggggcaat tattatcctt
    23641 cttggtttgc accaaatgga gattctacac tttaaggggc tttataagga aaagagacta
    23701 caactgcaag gacaggggca aaatggtaag ggctatagtc aggcattttt attgggcttg
    23761 acctttagtt ttgcttggac gccttgcgtg gggccggttc tggggtctgt tttggccttg
    23821 gcggcttcag gtggttcagg agcttggcag ggagctggtc tcatgttggt gtatacgctg
    23881 ggcttggcgc taccattctt gcttctagct ctgacctcta gttatgtttt gaaacatttc
    23941 cgaaaacttc atccctatct cggaatcctc aaaaaagtgg gtggttttct cattattgtg
    24001 atgggactct tggttctgtt tggaaatgct tcaattttaa gtcaattatt tgaataaaat
    24061 ggaaaggaat atcaatatga aaaaatggca aacatgtgtt cttggagcag gttcgctcct
    24121 ttgtttgacg gcttgttcag gcaagtccgt gactagtgaa caccaaacga aagatgaaat
    24181 gaagacggag cagacagcta gtaaaacaag cgcagctaaa gggaaagagg tggctgattt
    24241 tgaattgatg ggagtagatg gcaagaccta ccgtttatct gattacaagg gcaagaaagt
    24301 ctatctcaaa ttctgggctt cttggtgttc catctgtctg gctagtcttc cagatacgga
    24361 tgagattgct aaagaagctg gtgatgacta tgtggtcttg acagtagtgt caccaggaca
    24421 taagggagag caatctgaag cggactttaa gaattggtat aagggattgg attataaaaa
    24481 tctcccagtc ctagttgacc catcaggcaa acttttggaa acttatggtg tccgttctta
    24541 cccaacccaa gcctttatag acaaagaagg caagctggtc aaaacacatc caggattcat
    24601 ggaaaaagat gcaattttgc aaactttgaa ggaattagcc taggaggtgt cttatgaatg
    24661 ataagttaaa aatcttcttg ttgctaggag tattttttct agccataacc ggtttctatg
    24721 ttctattgat acgaaatgca gggcagacag atgcctcgca aattgaaaag gcggcagtta
    24781 gccaaggagg aaaagcagtg aaaaaaacag aaattagtaa agacgcagac ttgcacgaaa
    24841 tttatctagc tggaggttgt ttctggggag tggaggaata tttctcacgc gttcccgggg
    24901 tgacggatgc cgtttcaggc tatgcaaatg gtagaggaga aacaaccaag tacgaattga
    24961 ttaaccaaac aggtcatgca gaaaccgtcc atgtcaccta tgatgccaag caaatttctc
    25021 tcaaggaaat catgcttcac tatttccgca ttatcaatcc aaccagcaaa aataaacaag
    25081 gaaatgatgt ggggacccag taccgtactg gtgtttatta cacagatgac aaggatttgg
    25141 aagtgattaa ccaagtcttt gatgaggtgg ctaagaaata cgatcaacct ctagcagttg
    25201 aaaaggaaaa cttgaagaat tttgtggtgg ctgaggatta ccatcaagac tatctcaaga
    25261 aaaatccaaa tggctactgc catatcaatg ttaatcaggc ggcctatcct gtcattgatg
    25321 ccagcaaata tccaaaacca agtgatgagg aattgaaaaa gaccctgtca cctgaggagt
    25381 atgcagttac ccaggaaaat caaacagaac gagctttctc aaaccgttac tgggataaat
    25441 ttgaatccgg tatctatgtg gatatagcaa ctggggaacc tctcttttca tcaaaagaca
    25501 aatttgagtc tggttgtggc tggcctagtt ttacccaacc catcagtcca gatgttgtca
    25561 cctacaagga agataagtcc tacaatatga cgcgtatgga agtgcggagc cgagtaggag
    25621 attctcacct tgggcatgtc tttacggatg gtccacagga caagggcggc ttacgttact
    25681 gtatcaatag cctctctatc cgctttattc ccaaagacca aatggaagaa aaaggctacg
    25741 cttatttact agattatgtt gattaatact cttcgaaaat ctcttcaaac cacgtcagcg
    25801 tcgccttacc gtatgtaggg ttactgactt cgtcagtttt atctacaacc tcaaagctat
    25861 actttgagct gacttcgtca gttttattta caacctcaaa gctatacttt gagaaacctg
    25921 tggctatctt cctagtttac tttttgattt ttattgagta taagaggtct ttcctaagca
    25981 gttagaggag aattttgcta tactgatact agtaagtgac aaaggagaga agcatgacct
    26041 acacaatctt aatcgtagaa gatgaatatc tggtaagaca aggtttgact aaactggtca
    26101 atgtagcagc ctacgatatg gaaatcatcg gtcaggctga aaatggaagg caggcttggg
    26161 aattgatcca aaagcaggtg ccagatatca ttttaaccga tatcaacatg cctcatctaa
    26221 atggcatcca gttggccagt ctggtacgag aaacctatcc tcaggttcat ttggtctttt
    26281 taacaggtta cgatgatttt gattatgcct tgtctgctgt caaactaggt gtggacgact
    26341 acctgctcaa acccttttct cgtcaggata ttgaggaaat gttggggaaa atcaaacaaa
    26401 aactagacaa ggaagagaaa gaagagcagt tacaagattt attgactaat aggtttgaag
    26461 gaaacatggc ccagaaaatc cagtctcatc tggctgatag ccaatttagt ttaaagtctt
    26521 tagccagtga cttaggtttt agtccgacct atctgagttc cttgattaag aaagagttgg
    26581 gcttgccttt tcaggattat ctggtgagag aacgtgttaa acaagccaag ctcttgcttt
    26641 taactacaga tctgaagatt tatgagatcg cagagaaggt tggttttgaa gatatgaact
    26701 attttaccca acgttttaag cagattgcag gtgtgacacc tcgtcagttt aagaagggag
    26761 aagaccgatg aagcgttctt ctcttttagt tagaatggtt atttccatct ttctggtctt
    26821 tctcattctc ctagctctgg ttggaacttt ctactatcaa tcaagttctt cagccattga
    26881 ggccaccatt gagggcaaca gccaaacgac catcagccag actagccact ttattcagtc
    26941 ttatatcaaa aaactagaaa ccacctcgac tggtttgacc cagcagacgg atgttctggc
    27001 ctatgctgag aatcccagtc aagacaaggt cgagggaatc cgagatttgt ttttgaccat
    27061 cttgaagtca gataaggact tgaaaactgt tgtgctggtg accaaatctg gtcaggtcat
    27121 ttctacagat gacagtgtgc agatgaaaac ttcctctgat atgatggctg aggattggta
    27181 ccaaaaggct attcatcagg gagctatgcc tgttttgact ccagctcgta aatcagatag
    27241 tcagtgggtc atttctgtca ctcaagaact tgttgatgca aagggagcca atcttggtgt
    27301 gcttcgtttg gatatttctt atgaaactct ggaagcctat ctcaatcaac tccagttggg
    27361 gcagcagggc tttgccttca ttatcaatga aaaccatgaa tttgtctacc atcctcaaca
    27421 cacagtttat agttcgtcta gcaaaatgga ggctatgaaa ccctacatcg agacaggtca
    27481 gggttatact cctggtcaca aatcctacgt cagtcaagag aagattgcag gaactgattg
    27541 gacggtactt ggcgtgtcat cattggaaaa gttagaccag gttcggagtc agctcttgtg
    27601 gaccttgctt ggggccagtg tcacatctct tcttgtctgt ctctgcttag tgtggttcag
    27661 tcttaaacgc tggattgctc ctttgaagga tttgagagaa accatgttgg aaattgcttc
    27721 tggtgctcaa aatcttcgtg ccaaggaagt tggtgcctat gaactgagag aagtaactcg
    27781 ccaatttaat gccatgttgg atcagattga tcagttgatg gtagctattc gtagccagga
    27841 agaaacgacc cgtcagtacc aacttcaagc cctttcgagc cagattaatc cacatttcct
    27901 ctataacact ttggacacca tcatctggat ggctgaattt catgatagtc agcgagtggt
    27961 gcaggtgacc aagtccttgg caacctattt ccgcttggcg ctcaatcaag gcaaggactt
    28021 gatttgtctc tctgacgaaa tcaatcatgt ccgccagtat ctctttatcc agaaacaacg
    28081 ctatggagat aagctggaat acgaaattaa tgaaaatgtt gcctttgata atttagtctt
    28141 acccaagctg gtcctacaac cccttgtaga aaatgctctt taccatggca ttaaggaaaa
    28201 ggaaggtcag ggccatatta aactttctgt ccagaaacag gattcgggat tggtcatccg
    28261 tattgaggat gatggcgttg gcttccaaga tgctggtgat agtagtcaaa gtcaactcaa
    28321 acgtggggga gttggtcttc aaaatgtcga tcaacggctc aaacttcatt ttggagccaa
    28381 ttaccagatg aagattgatt ctagacccca aaaaggaacg aaagttgaaa tatatataaa
    28441 tagaatagaa actagctaac tcccagtcaa ttctgggagt tttatgcgta attgggcaaa
    28501 ctttctaggt tgtctatctt gctaaaaaca agaaaaaacg gtatgataga agagtgacaa
    28561 cagaggtaac agagatgaac gagacacaga ttcaaagaga aacacgtcag gtagtggaag
    28621 atgttttaga aaagaccaat ttgaaacagg gggccctttt tgttttaggt ctatcttcta
    28681 gtgaggtgct aggtggtcag attgggaaag aatccagtca agagattggt gagctgatag
    28741 tggagactat cttgggtatt ctaggtagta gaggtatcca tttagcagtt caaggttgtg
    28801 agcatgtcaa tcgggctctc gttgttgaac gtcaggtggc agagcagttt ggtctggaaa
    28861 ttgtcagtgt ccatcctact cttcatgcag gaggttcggg gcagttggca gcctttaagt
    28921 ttatgcagga tccagttgag gttgaattta tcaaggctca tgctggattg gatatcggag
    28981 acactgcaat tggcatgcat gtcaagcatg ttcaggttcc gattcgccct attttgagag
    29041 agattggtca tgcccatgta acggcactgg ctagtcgtcc aaaattaatc ggaggtgcgc
    29101 gtgcgcacta tccgcaagat gctattagaa agacctgaga atgaataaag gagataaaag
    29161 tgtttaaaaa agaccgtttt tcaattcgta agattaaggg agttgtaggc tctgtatttc
    29221 ttggaagcct tttgatggct ccttctgtag tggacgcagc cacctatcac tatgtaaata
    29281 aagagattat ttcacaagaa gctaaagatt taattcagac aggaaagcct gacaggaatg
    29341 aagttgtata tggtttggtg tatcaaaaag atcagttgcc tcaaacaggg acagaagcat
    29401 ctgttttgac agcttttggt tatctatcag gtgacatcct caaaacgctt ggcttggaca
    29461 ctgttttaga agaaacctca gcaaaacctg gagaggtgac tgtagtcgaa gttgagactc
    29521 ctcaatcaac aacaaatcag gagcaagcta ggacagaaaa ccaagtagta gagacagagg
    29581 aagctccaaa agaagaagca cctaaaacag aagaaagtcc aaaggaagaa ccaaaatcgg
    29641 agataaaacc tactgacgac acccttccta aagtagaaga ggggaaagaa gattcagcag
    29701 aaccagctcc agttgaagaa gtaggtggag aagttgagtc aaaaccagag gaaaaagtag
    29761 cagttaagcc agaaagtcaa ccatcagaca aaccaacaga ggaaccaaaa gttgaacaag
    29821 taggtgaacc agtcgagcca agtgaagacg aacaggcacc aacggcacca gttgagccag
    29881 aaaagcaacc agaagctcct gaagaagaga aggctgtaga ggaaacaccg aaaccagaag
    29941 ataaaataaa gggtattggt actaaagaac cagttgataa aagtgagtta aataatcaaa
    30001 ttgataaagc tagttcagtt tctcctactg attattctac agcaagttac aatgatcttg
    30061 gacctgtttt agaaactgca aaaggtgtct atgcttcaga gcctgtaaaa cagcctgagg
    30121 taaatagtga gacaaataaa cttaaaacgg ctattgacgc tctaaacgtt gataaaactg
    30181 aattaaacaa tacgattgca gatgcaaaaa caaaggtaaa agaacattac agtgatagaa
    30241 gttggcaaaa cctccaaact ggggttacag aagctgaaaa agttgcagct aatacagatg
    30301 ctaaacaaag tgaagtaaat tctgagactg caagtcttaa aacagcaatt tctagattaa
    30361 atacagataa agttgaactc gaaaatcaac ttaagattgc acagggaaaa actgaaactg
    30421 atttcagcat ggaatcttgg actgttttga gtacagctaa aaataaagct caggaagtaa
    30481 aagataacgg aacagcgacc caagaacaaa taaacgaagc agagaaaagt ttgaaaacag
    30541 cccttgctga cttatctgtt gacaaaacag ctttgggaag cgctatcgat acagctacta
    30601 agaaaaataa agagaattat acaaatcaaa catgggcaga gttggaaaca gcgcttacag
    30661 cagcaaaatc tgtaaatact aacgagtcta aacaaagcga cgttaacgaa gctgctgaaa
    30721 aattaactgc aactatggaa aaattggttg aattgtctga aaagccacgt ttgactctat
    30781 ctattgaaaa gagagacata gatcgaaaag ctacggtcac gtatactttg gaaaatccag
    30841 caaatactca aataaaatca ataactgcaa ctctgaaaaa aggagaggaa gttgttaagg
    30901 attttgtttt aacagaagaa aatcttaaaa ctaatcattt aactgctttg tttgaaaagc
    30961 tagactacta caaagaatac actctatcta cggatatggt atataacaga ggaaatgatg
    31021 atgagacgga gagtatttca gaagaactaa ttcagctgaa tcttaagaaa ctagaattga
    31081 aagatatcca gacagtctct ctgatgaaat ttgaaaatgg tcaagaaagt caggttacac
    31141 acttgtctga taaaccgacc gatttgagta aattatactt aaaagttaca tcatcaacgt
    31201 ctaaggatgc tgtgttagcc gttagctcta tagaagaaga gattattgaa aataaaaaaa
    31261 tctttaaaat tcatgcagat acaccagaat tagtagttcg taagaaagat ggcagtcttt
    31321 ctaagggatt tgactattac atggagagag ttatccctca tgatggtgat atttattatg
    31381 attttaaaga cttgatttca gctatgacat ctaatcctac aggtacgttt attttaggga
    31441 gagatatttc ctcgcgtaat gtaaaaccgg atggaaatgg aaaatcttat atcaaaggtg
    31501 aatttaaagg aaaactactt ggtacaaatg ataatgttag acactcgatc tttgacttag
    31561 aatatccatt gtttgatact ataaaatcag gagtcgtgaa agatattgat ttcaagcatg
    31621 tcaatatggt attcccagat agcaatcaag gagataacgt agcaacaatt gcaagagtaa
    31681 tcaaagataa gacaaaaatt gagaatgtta atgttgaagg ttatctcgag ggtcgagatc
    31741 atgtagctgg tttagttaat aatcttgaag gtaattccga aattgaaaat gtatccttta
    31801 caggaaagat taagtctaaa ggtggtaatt ctattacagc aggtattgca ggtagaaata
    31861 tcttatctcg tgttaaacgt gcctatgtaa atgcagatat agaggtacat cgatcatcta
    31921 attctagcat gcttgttgca gtaaatggaa taaacgcaga tgcaagtggt ggttggggaa
    31981 cctggggtag acttactgag tcagttgcta aaggaacatt agaaaccaag caaggagggc
    32041 aagctggagg agcctcttct acagtctggc cttatggagc aattgataat gttgtcagct
    32101 atgcaaaagt gaccaaaggg aaagaattat ttggatcaga tggtgattta aattatgatt
    32161 ggtttatgaa aaaaattagt aatatttttg gtgttcaagg catcagctca ggcgattcgg
    32221 gtagtgattc taaatttacg cgtatttctg aggaagaagc aaatcaaaaa gttgcttctt
    32281 ataatattac agctcccaac ttgatgagtg actcaagtct tttggtagac agactcaatg
    32341 aaagttggaa aaatactgat caatttgaaa gtatacagga ttatcaagcc caaaaccaat
    32401 taatttacca gaacttgact aaatttactc cttattacaa taaagaattt attgttcatg
    32461 aaggaaatgc tctcactcct gaacaggaaa tattgaagac taagaaaatc aaatcgattg
    32521 tagggttgaa aggaacagag ttcgttgtag atggcagtga tattgatact atcatgcttc
    32581 attttgagga tggtagtcag aagcgatata aggtaacctc tactggtaag ttttcgataa
    32641 ctaatttacc ggaatatcag gttgaagatt taaatgtggt gtatacatca gaacatatag
    32701 tacatccact agattcgtca ctgataaata accttgttga agaattgaaa aaagtagaat
    32761 tgtatacaga gtctacttac caagttctag gtattgataa agataatgct aacaaattga
    32821 atagaactaa gagattattc ttagatgagt cattagatgc tgttaaaaca caattgccta
    32881 cctttgtaaa aacaatgttt gaaaatgagt ggttgcacat caatggagaa tctagtggag
    32941 ctgtcgctgc tttacgacaa aaaatcatgg ataataagac ggctatattg cttgctttga
    33001 cttatatcaa tcgttattat gatgttaaat tctcagacta taatattaaa aaactgatgc
    33061 tctttaaacc aactttccat ggtgaaaaga ttgatctatt agatcgatta atccgactag
    33121 gatcatctgg agaaaataga ttaaaaggtt cagaaaatgc agaaacgttc aagcagttat
    33181 ttgctagcga aacgaagcag aaggatttgg tgacttattt agactacaat cgctctttgt
    33241 tgactaatta tcaaacaaca ggggaatggt ttaaagaaac gactaaggat tatattcaat
    33301 ttgaggaacg tccatcctta gtagaggaga ttaaagatgc caaatatcga gtttacgata
    33361 atttaaccgc accgtattat caaggatata tcctacctct cttaacgttg aagaatactc
    33421 atttagcaat tttatcgaac tatagtacta tgacatttgt cagccgtgaa aaacgcccta
    33481 attggaaaaa tgaagatttt gataagtggg tgaaatatgt agcaactgct catagaaacc
    33541 atgttgatac ttggtataag attttacctg ataatatcaa aggtaaaatg gttaaagaga
    33601 atgttactgc tgtttgggaa ggattgagca tcccaggaag tgaatgggta gaccagaatg
    33661 ctgtagatag aaaaggaaga gattacgccc cagctcgaga attctttaat cttgtaggtg
    33721 gtcctatggg aggttggtat gcttaccatg gttatggtgc gcatgctgga ggaagaaatc
    33781 gggttaatta tgaggtgttt gatgtattaa gtgagtatgg tatatcggtc tttactcatg
    33841 aattgactca cgttaatgat acttggattt acttaggagg gtatggtaga cgtgaaaata
    33901 tgggacctga agcttatgct cagggattat tccagtctcc tgttccaggt cagccgggtt
    33961 ggggggcgct tggtctcaat atggcatttg aaagaaaaaa tgatggtgat ctaatttata
    34021 atgcctcccc aactcaattt gaaaatagga aagagttaga tagttatatg aaaaattata
    34081 acgatactct catgatggtg gactacttgg aaggagatgc agttatctct aaaggtaaag
    34141 aagctattac taaatggttt aaaaaagttg aacctaaggt tgttagtcaa acagctcagt
    34201 atgatacggt tcgtcagttg actgctgaag agaaagaaaa actttctgtt tctagtgttg
    34261 atgatttagt tgatcaagga ttaatgtcag acagagctgt aggaaataat acctataatc
    34321 cagctgattt tgaaacaagt tatattgcga tagattatat gactggtatt tatggtggag
    34381 gaaagaatag tgtaggttca ccaggtgcct tgatgtttaa acataatact ttccgtatgt
    34441 ggggctacta tggttttgaa gaaggtgttc taggatatgc ttctaataaa tttaagcaag
    34501 catctagaga tgaaggtcat gctggactta gtgataactt tattatttct aagatttcca
    34561 aaggggaatt tttaactatg gaagcattta aaaagggata ttttaagaaa gtagtagaag
    34621 agttaaaaac taaaggtatt cgtcctgtta ctatcaatca aaaaacatat agcacctttg
    34681 aagaattgca agaaggattt aagcaggctg ttgaaagaga tttgaagaaa aatcagttag
    34741 atgagaggga aacaagaaac ttcaaattcc aagttttcag acaacttctt caacaaacag
    34801 atagctttaa aacttctatc tttagataat cgaaatctct catctgcttt gcggatgagg
    34861 gttttcttat tttatgctat acttaaagta tgcatagaaa aacagtgatt gattttagga
    34921 ctttggggga gagatatacc tttacccagc ctattaaaga gttgaaaacg agaaatgtag
    34981 aagaagtggc agatttgctg gcacaagtgg aaagctacca agagcaaggt tattatgtgg
    35041 tggggtatgt cagctacgag gctgcacctg cttttgagga aaaattagca gttcacaagg
    35101 atcccctact gggagagtac ctgctttatt ttactgttca tgatagcgta gaaacttccc
    35161 ctattcctct gacttatgag gatattgatt tgccctcaaa ttggcaggaa gtaacgtctg
    35221 cagcagacta tgaaaaggcc attgcccaaa ttcaccatca tttacggcag ggagatacct
    35281 atcaggtcaa ctacaccgtc caacttaagc aaaagttaaa tgccaatcct tttgccatct
    35341 acaatcgtat ggtggtagag caggaggcgg gctacaatgc ctatgtggaa catgacgaga
    35401 tggcagtgat ttccatgagc ccagagctct tttttgagca aaatgatcgc gagttgacaa
    35461 cacgaccaat gaaggggacg actcagcgtg gggtaactga ccaagaagat cttgaacagg
    35521 ccagttggtt ggaacaggat cccaaaaatc gctctgaaaa tatgatgatt gtggacctct
    35581 tgcgcaatga tatgaaccgt atttctgaag ttgggagcga gcacgtggag cgtctgtgtc
    35641 aggtagagca gtattcaact gtttggcaga tgacttcgac catcaagagt cagttgcgag
    35701 aggatgtgga ccttgttgaa atcttccact cactctttcc ttgtggttcc ataacgggtg
    35761 caccgaaaat tgcgacaatg gagattatca aggacttgga gcctcaaccg cgtggagtct
    35821 actgtggaac gattggtctc ttgcttccaa atggacgacg gatttttaat gtggccattc
    35881 gtaccattca acttcacaaa ggtcaagcca tttatggagt tggcggaggg attacttggg
    35941 atagcacttg ggaatctgaa taccgtgaag ttcatcaaaa ggcagcagtt ctctatcgta
    36001 aacaagctcg cttccaatta attacaactg gaaaaatcag ccaaaaacaa ctgatgtttg
    36061 aagaacaaca tctggaaagg ctgagaaaag ctagtcggta ttttgcttat ccttttgacc
    36121 cagaaatttt gagacaaaag attgaagcag agtgtcagac ttgtgatgct aatcaagatt
    36181 accgcttgcg aatttctctc agcaagtctg gagagataga attcagtcgt caaatcttaa
    36241 cacctcttag tccaagtttt tgtcaggcca aactttgtct gcaagaagcg aatttgaatc
    36301 aatcctttac ctattttaaa acgacttaca gaccgcattt gagcatagag gaacaagaga
    36361 agatttacca taatggaaaa ggagaacttc ttgaaacctc tatcggaaat ttggttctga
    36421 aaatcgatgg gaaactctac acaccgccta tccgacttgg aatcttgcca ggaatttatc
    36481 accagtattt actagaaaca ggacaggtag aagagaaagt cttgaccttg gcagattttg
    36541 cccaagcaga agctatttac ggctgtaatg cagtgagagg cttgtatgag ttgcaagtga
    36601 taggattgtg agttttagct attaatagtt tttaatatat attaaaatat attggtgttt
    36661 acttttgatg agatatcata tttgtgttat aatgtaaccg taaacaatag aaatttaaag
    36721 gagatttttg atgaaatcaa aaatagtttt gggagcttcg ctagcgatcg caacacttag
    36781 cttagtatct cttgtcgaga ttgaaggact ctcaccgttt ttaatagaga atgttagcgc
    36841 aaatactcat tctgcaaata aagttattaa tcataaagta agtatttact tagaaaatgc
    36901 tgatgaaggt aaaggtctaa ctgtaaactt ttcaacagac agtgttagtc caaatttatt
    36961 tgatgagttc gaaaaaaaga gtggaattac aataactact atgttagtga atgctaagac
    37021 aggtgaagtt gtagaaaaaa gattaacacc aagcgttttt ttacgttcca atgatttaac
    37081 cagtgggacc atatcttcgt ttatttttag tgaatatcct gatggagaat ataaatatgt
    37141 tgtgtctaaa ggagacttta tcgatcctaa gactcagttt aaacatcagt atagaggaga
    37201 atctccagtt tttagaatac gtaatcgaaa atatgtggag ttgggaacaa ctgataaaaa
    37261 attagatgaa agacgtgata atagcgttta taaagatggg gttgttgaac acaaggtaaa
    37321 tttaagttta acaagttatc aaggaggtaa tggagttact gcaatttttt caactgatag
    37381 tgttaactct aacttactta attcttttgg tgaaaaggct aaaaaagttt tgattaggtc
    37441 taagttaatt aatgttaaga ctggcgaggt tattgatgaa acgttttctc caaaagtaag
    37501 tttaacctct aaaattttga aaagtggttc tacagcagta ttttatttta tagatttaac
    37561 tgatggcgaa tataaatatg ttgcgtatga aagccagcaa tatactgatc ctcaaacaac
    37621 attgactcat cagtatagag gagaatctcc tatttttagt atcaaagatg gaaaattttc
    37681 tggtttagta tctgcctcaa aaccagacga aaacccgaag ccaacgccaa aaccggatga
    37741 gaaaccaaaa ccttcagcac cacaacagga gaaaccaaaa cctacagttc agtctggttg
    37801 ggtaggttca tcttattatc aaaatggtaa aaaagtcact agtaagtgga tttttgataa
    37861 aaaatataat tcatacttct atctagatgc atcaggaaat tatgttcaaa acgcttgggt
    37921 aggtaactat tatctcaaat caggcggata catggctaaa agtgagtgga tttatgataa
    37981 gaactatggt tcatactact atctaacatc agaaggcagc tacgctcgta acacttggtc
    38041 aggcaattat tatttgaagt caaatggtaa gatggctaaa agtgagtggg tatacgatag
    38101 taactacaaa tcatattact acttgacatc agaaggaagt tatgctcgta acacttgggt
    38161 aggcaattac tatcttaaat caaacggtaa aatggctgtt aatgaacgta caccagatgg
    38221 ttatcgtgta gatggttcag gtaaatgggt taaataagaa actaaaaaat aggataatag
    38281 gtaatatcag atgaataaaa aaaattattt tcatgtgctt tagcattttc aacgattgga
    38341 acattgtcaa cgattgataa atcaggagtt cttccttttt cagcgcaaac ggtgcaggct
    38401 gacatacaaa cgcttacccc aatggcaaaa tattatatta ttcttccgaa ggatgcagaa
    38461 atatataaaa cttggagagg tactgtcaat attcccatta ttgatgctac aaaaactact
    38521 ccagaattat cttatttcaa agaagatcat cgcaactata tagctaatga gaataagtct
    38581 ggtgccaatt acatagagtg gaagggaaca gtagaagagt ttaaagaggc cattaagaag
    38641 ttgactgata aaaaatcaac tactgcaaca ccaaaaaagg atgaaaagcc aacaccaaaa
    38701 ccggatgaga agccaaaacc aacacctaca gttcagtctg gttgggtagg ttcatcctat
    38761 tatcaagatg gtaaaaaagt cattagtaag tggatttttg ataaaaaata taattcatac
    38821 ttctatctag atgcatcagg aaattatgtt caaaacgctt gggtaggtaa ctattatctt
    38881 aaatcaggcg gatacatggc taaaggtgaa tgggtttatg atgccaccta tcaagcttgg
    38941 tattatttga catcagatgg ttcttatgct tacagtacat ggcaaggaaa ttactatcta
    39001 aaatcggatg gtaaaatggc tgtcaatgaa tgggttgatg gtggacgtta ttatgttggc
    39061 gctgacggag tttggaagga aggtcaagca agtacagctt cttctagtaa tgatagcaat
    39121 agtgaatatt ctgctgcttt aggaaaggca aaaagttata attcgttatt ccacatgtca
    39181 aaaaaaaacg tatgtataga caattaactt ctgattttga taaattttca aatgatgcag
    39241 ctcaatatgc cattgatcat tagatgatta acagatactg gtaggcgaaa aaattcctta
    39301 taattcactt acgcaaacct ttgcataagt gaatggattt tgatatacta gtactgtaag
    39361 cattttcaat tactacacaa tgaaaaagga gaatatgatg agtcaaaaga ttattgggat
    39421 tgaccttggt ggaacttcta tcaaatttgc aatcttaaca acagcaggag aaatccaagg
    39481 aaaatggtca atcaagacca acattttgga tgagggaagt catatcgttg atgatatgat
    39541 tgagtctatt caacatcgtt tggacttgct tggattggca gcagcggact tccaaggcat
    39601 tggaatggga tcaccaggtg tggttgaccg tgaaaaaggg actgttatcg gtgcctacaa
    39661 cttgaactgg aaaacccttc aaccaatcaa acaaaagatt gaaaaagcct tgggcattcc
    39721 atttttcatc gataatgatg ccaacgtagc agctcttggt gagcgctgga tgggtgctgg
    39781 agataaccaa ccagacgttg tctttatgac actcggtact ggtgttggtg gcggtatcgt
    39841 cgcagaaggc aaattgcttc acggtgttgc tggtgcagca ggtgagttgg gccacatcac
    39901 tgttgacttt gaccagccaa ttgcatgtac ttgcggtaag aaaggctgcc ttgagacagt
    39961 tgcttcagca acagggattg tcaacttgac tcgtcgctat gccgatgaat acgaaggcga
    40021 tgcagccttg aaacgcttga ttgataacgg agaagaagta actgctaaga ctgtctttga
    40081 gctcgcaaaa gaagaagacg accttgcttt gattgtttac cgtaacttct cacgttactt
    40141 gggtattgca tgtgctaaca ttggttcaat cctaaaccca tcaacaatcg tcatcggtgg
    40201 tggtgtgtca gctgcaggag aattccttct acaaggtgtt caaaaagtct acgatgaaaa
    40261 tagtttccca caagtacgca catctactaa attagcactt gcaactctag gaaatgacgc
    40321 tggagttatc ggagcagcat cacttgtatt gcaataagat aatgagagag gaaaatgcta
    40381 acctagagtt agtaatgttc ctcttttatt ttttatgcta tactagttag gacaatgaat
    40441 gaggtgaaac gaaatgacaa aagcagatac gatttttaaa gagaatattg aacgcattct
    40501 aaaggaaggt gttttctcag agcaggctcg tcctaagtac aaggatggga ctgttgctaa
    40561 ttccaagtat gtaactggtg ccttttccga gtacgactta tctaaaggag aatttcctat
    40621 cacaaccttg cgtcccattg caatcaaatc cgccatcaaa gaagttctct ggatttacca
    40681 agaccagtca aatagcttag aagtgcttaa tgacaagtac aatgttcact actggaatga
    40741 ctgggaagtt ggagacacgg gaaccattgg tgagcgctat ggtgccgttg ttaagaaaca
    40801 cgacattatc aataagcttc tcaaacagtt ggaaaccaat ccttggaacc gccgcaatat
    40861 tatttcgctc tgggattacc aagctttcga agaaacagat gggctgctcc cgtgcgcctt
    40921 tcagaccatg tttgatgtcc ggcgtgttga tggggaaatc tatctggatg cgaccttgac
    40981 ccagcgctcc aatgatatgc tggtggccca ccacatcaac gctatgcagt atgtggcttt
    41041 gcagatgatg attgccaaac attttggctg gaaggttggg aagttcttct acttcatcaa
    41101 caacctccat atctatgata atcaatttga acaagctcag gaattgctcc gtcgggagcc
    41161 gtcaaactgc caaccacgct tggttttaaa tgttcctgat gggactaatt tctttgatat
    41221 caaagcagaa gattttgagt tggtggatta tgaccctgtt aagccacagt tgaagtttga
    41281 cctagctatt taaaagaata gaaaaaagaa gttgagaata atcccaactt cttttgtttc
    41341 ttaacgtgat acgcggcgac gagctgcttt tttacggttt tcttcgatga aagctgcttt
    41401 ttgctcttct ggttcgatta ctttcttttt aaatgcgtat actgcacctg caacggcagc
    41461 gacagttcct gcgacacctg ttacaagacc tttagcgaat cctttagcca tgagtcttcc
    41521 tcctttatat tctcaatcag ccagcctcct caagaggtca catttttctg actgaccttt
    41581 ttgtgttata ataatagtaa cgaaaaaatg ggaatttttc aaggaaaaaa gatgaaaaca
    41641 aaaataattg tgattgttgg accgactgct gttggaaaga cggctctagc tattgaagtt
    41701 gcaaagcgct ttaatggcga agtggttagt ggagatagcc agcaagtcta tcgaggactt
    41761 gatattggga cggccaaggc tagtccagaa gagcaggcag ctgttcctca tcatttaatc
    41821 gatgttagag agataaccga gtcttactcg gcttttgatt ttgtttcaga agctaagatg
    41881 actattgagg atattcagag ccgtggcaag ctagccatta tcgccggtgg gactggactt
    41941 tatatccaga gcttgctaga aggttaccac ctaggtgggg agactcctca tgaggagatt
    42001 ttagcttatc gagctagttt ggagccatat tcagatgagg aattagccca tttggtggag
    42061 caagcaggcc ttgagattcc ccagtttaat cgtcgtcgtg ctatgcgtgc cttagaaatt
    42121 gcccattttg gtcaggattt ggaaaatcaa gagatcttgt atgaaccgct gattatctgc
    42181 ttggatgatg aacgtagtca actttatgag cgtatcaacc accgagtgga tttgatgttt
    42241 gaggctgggc ttttggatga ggccaagtgg ctgtttgacc attcccctaa tgtacaggct
    42301 gctaaaggaa ttggctataa ggaactcttt ccttatttcc gtggagagca gacctttgag
    42361 gaagctcgtg agagtcttaa acaggcgacc cgtcgttttg ccaagcgtca gctgacctgg
    42421 ttccgtaatc gcatgcaggt caccttttat cagattggag aatctggtgt acaagaccgc
    42481 attttaagcc agatagagga gtttttagat gattgaaacg gagaaaaaag aggagcgagt
    42541 cctgctgatt ggtgtggaat tgcagggtat ggacagtttt gacctctcca tggaagaatt
    42601 ggctagttta gcgaaaacgg caggggcagt cgttgtagat agctacagac aaaaacgtga
    42661 aaaatatgat tccaagacct tcgtcggctc tggtaagttg gaagagattg cgcttatggt
    42721 ggatgcagaa gaaatcacta ctgtcatcgt caacaatcgt ctgaccccaa ggcagaatgt
    42781 caatctagag gaagttctcg gtgttaaggt cattgaccgt atgcagttga ttttggatat
    42841 ctttgccatg cgggctcgaa gccatgaagg gaagctccaa gtccacctag cccaactcaa
    42901 atacctcttg cctcgcttgg ttggtcaggg gattatgctc agccgtcagg cagggggaat
    42961 tggttcccgt ggtcctggtg aaagccaact ggagctgaac cgtcgtagcg ttcgcaatca
    43021 aatcacggat atcgagcgcc agcttaaggt ggttgagaaa aatcgtgcga ctgtcagaga
    43081 aaaacgtttg gagtctagca cttttaagat tggtttgatt ggttatacta atgctgggaa
    43141 atcaactatc atgaacatct tgaccagtaa gacccagtat gaagcagatg agctctttgc
    43201 gactctggat gcgacaacca agagtattca tctgggaggc aatctccaag taactttgac
    43261 agataccgtt ggctttatcc aagatttgcc gacagagttg gtgtccagtt tcaagtcaac
    43321 cttggaagaa agcaagcatg tggaccttct ggttcatgtt atcgatgcta gcaatcctta
    43381 ccacgaggag catgaaaaaa cggttctctc catcatgaaa gacctggaca tggaagatat
    43441 tcctcacttg acgctttata ataaagcgga tttggtggag gatttcacgc ctacccaaac
    43501 gccatatacc ctcatttctg ccaagtctga ggacagtcgt gaaaacttgc aagcattatt
    43561 gctagataag attaaggaaa tttttgaagc atttaccctg cgagtgcctt tttcaaagtc
    43621 ctacaagatt catgatttag agagtgttgc aattctggaa gaacgtgatt atcaggaaga
    43681 cggcgaagtg attacaggct acatttccga gaaaaataaa tggaggttag aagaatttta
    43741 tgactgatat taaaactttg gctctaaagt atggaggtta tacaagtctg gacaaggtct
    43801 atctggatca gcttctagct ggtaaaacag atcaggagca gttaacactt attacacctc
    43861 cgccaagtgt tgttaatgcc tactttgcag aactctatca gaaaaagagt cctcaggctg
    43921 cgacggatta ttttgcagaa ctcagtcagg aactgaatct ttacaatact gagccaagtt
    43981 tcaccctaga aaataagcct tttattcggc ttaatctgtc tggtaaatcc tttggttttt
    44041 gttatgagag tgacggcctt ggtcggattt tctttgaaaa tgaggagaaa atctcggatg
    44101 acttgctctt tgaaattgcg caaattttcc cccatcaatt agtctttgaa gagtctggta
    44161 agatttacat gaagcctgtc gaggacgagg aagttatcag cctagaaagt ctcacagctt
    44221 tgactgattt ggaaagattg gctgatggtc gaaaacgtct caaaggctac agccaagagg
    44281 atttattgca agaagctgct gctttttctg gcaaacgcta tttccgatcg gaaaaccgca
    44341 cagccatgtt atatattgat taattagaaa gtatcgaatg gatattcaat ttttaggaac
    44401 gggggctggt cagccctcta aagcccgcaa cgtttcaagt ctcgccctga aacttttgga
    44461 tgagattaac gaagtttggc tctttgactg tggagaaggt acgcaaaatc gcattctgga
    44521 aaccacaatt cgaccacgta aggtcagcaa aatctttatt acccatctgc atggagacca
    44581 catttttggt ttgccaggtt tcctttctag ccgtgccttt caggccaatg aagagcagac
    44641 agatttggaa atctacggac ctcaaggaat caagtcattt gtcttaacca gccttcgtgt
    44701 gtcaggttct cgtctgccct accgcattca tttccatgag tttgaccaag attctctggg
    44761 taaaattctt gaaaccgata aattcactgt gtatgcagag gagctggacc acactatttt
    44821 ctgtgttggc tatcgtgtca tgcaaaagga tctagaaggg acgctggatg ctgaaaaact
    44881 caaggctgct ggtgttccgt tcggcccgct ttttggtaaa atcaaaaacg gccaggatct
    44941 tgttttggaa gacggaactg aaatcaaggc agcagactat atctcagcgc cacgtccagg
    45001 taagattatc actattttag gagacactcg aaaaacgggt gccagtgtgc gtctggctgt
    45061 caatgcagat gtcctagttc atgagtccac ttatggcaag ggtgatgaaa aaattgctcg
    45121 taaccatggt cactcaacta atatgcaagc tgcacaagta gcggtagaag caggtgccaa
    45181 acgcctccta ctcaaccata tcagtgcccg tttcctctca aaagatatta gcaaactcaa
    45241 gaaagacgct gccacaattt ttgaaaatgt ccatgtggtc aaagacttgg aagaagtgga
    45301 aatctagcag tcacagaaag gataagtatg cctactattc tcattaccgg agctagcggt
    45361 ggtctagctc aagaaatggt caaactcctg cccaatgacc aactcatctt gcttggtaga
    45421 aataaggaaa aattagccca actctacgga aattattccc atgcagaatt gattgaaatt
    45481 gatattaccg acgattcagc cctagaagct ctggtaactg atctttatct ccgctatggc
    45541 aagattgatg tcttgattaa caacgctggt tacgggattt ttgagggatt tgaccagatt
    45601 gctgataaag atattcacca gatgtttgag gtcaatacct ttgccctgat gaatctgtct
    45661 cgtcaccttg cggctcgtat gaaggaaagc agcaaagggc atatcatcaa catcgtcagc
    45721 atggcaggtc taatagctac tggcaagtct agtctttact cagcgaccaa gtttgcggct
    45781 attggttttt caaatgctct gcgactcgaa cttatgccct atggagtcta tgtgacaaca
    45841 gtcaatccag gtccaatccg aacaggattt ttcgaccaag ccgacccaga tggaacttat
    45901 cttaaatcgg ttgaccgctt cctgctagaa ccagatgcag tggctaaaaa gattgtcaag
    45961 attataggaa aaaacaaacg agaactcaat ctcccgattt tgttgaacct agctcataag
    46021 ttttataccc tctttcctaa gctagctgat aagttggcag gggaaacttt taattataag
    46081 taaaaagaac caatgtgcag gttgttgcta gcctacatat tggttcttat catttctcaa
    46141 ctatcaaact gaacttcttc taggagatag tctataaagc gttcgcccat cttagatagg
    46201 ctggttttct catgctggat atagaccagc tcgatcgggt catcaatatc cagtgggata
    46261 gaaacgatat tgtctccgtt taggttgctg ttcaaaatcc ctgtcgcaat ggtataacca
    46321 tccaaaccaa tcaagagatt aaagagggtg gcacggtcac tgaccacaat ggatttcttg
    46381 tggtgttctt gagaaagaat ctcttctgaa aagtagaagg agttgtgcgt cccttggtca
    46441 tagctgaggt aagggaaatt ctccaaatca gacagtttca ccttgtcttt ctttgccaga
    46501 gggttggtct tgctgacaaa gatatgtggt tgcgctgtga agagatggtg ggctagcagg
    46561 tgattgtcat ccagcatctt ggttaaaaca tcacggttgt aactgtttaa gaagaggacc
    46621 ccgacctcac tgcggaagtt cttgacgtcg tcgataatct cccaagtccg agtttcacga
    46681 aggaagagtt cgtatttctc catatcgctt ttcttgagca aagagacaaa ggcattgacc
    46741 acaaaggcat agtgttgaga cgaaacgcta aatagttcgc ggtgggcgac aggattttta
    46801 tagcgttcct ccagaagctg ggtctgctcg acaacctgac gggcataaga gagaaactcc
    46861 atcccatcac gggtcaaggt gattcccttg ggattgcgga taaagatctc aatgcccatt
    46921 tcattttcca aatctcgcac tgcattggag agacttggct gagtgataaa gagttgcttg
    46981 gctgcctcat tcatggagcc agtttcgacg attttgataa tataatgtaa ttgttgaatt
    47041 ctcatgtttt tattgtacca tacttgcctt agaatcgcta aagaagatag gaaaataagg
    47101 agcattgtgt gagtctcttc ttgaaaaaaa cacaaaattt tgctagaatg agtcttatga
    47161 aaacattcta tgatgtgcag caattcctca aacgatttgg tattattgtt tacatgggaa
    47221 aacgcttata tgatattgaa ctgatgaagt tggaactatc tcggatttac gatgcagggt
    47281 tgatggataa attagactat ctagaggcag aagcagttct tcgcagagag cacaaggtag
    47341 aattggatta tattgagaaa aatggagaaa agaactaatg gttacttgga ttttgtgggc
    47401 acttatacta gcaatgttgg cgtggatggg ctttaactat cttcgtattc gccgtgcggc
    47461 taaaattgtg gacaatgagg agtttgaagc cttgattcgt acgggtcaat tgattgattt
    47521 gcgcgaccca gcagaattcc acagaaaaca tatccttggt gcacgcaata ttccttcaag
    47581 tcagttgaaa actagtcttg cagcccttcg taaagataaa cctgtccttc tctacgagaa
    47641 ccaacgtgcg caacgagtta caaatgcagc tctttacttg aaaaaacaag gtttttctga
    47701 gatttatatc ctttcttatg gcttggattc ttggaaaggg aaagtgaaga ctagctaacc
    47761 ttatttccaa attagtcgaa tgataggatg aagctattgt tttatagtat aattaatttg
    47821 tataaaattt aaggagttct tagaacataa aagaaaagaa gaaaaatcca ctttttgtgg
    47881 gtatcttgtc gattattctt ggtttgttat ttccaattgt aggcctgatt ttgggtatca
    47941 taggattggt cttggctatt tcataccaaa aagagtcata attagactat aaaatagaaa
    48001 agattcttaa tatcttagga attgtgattt ctgtagttaa ctggattgta gcaatcgcct
    48061 taatttttcg gtaaataaaa gaaaaaagct ttagttttaa agcttttttt gttttatcga
    48121 ctcatctcta aatagttttt aatgatttgt aactcttttt ggttcaaagg gcggtagtta
    48181 ctttctgcta aatctgggtt caatgtgaag tccccaaatt ggattctctt gaggatagtc
    48241 accttaacac caaccgagag gaacattttc ttgatttgat gaaattttcc ttctgaaatg
    48301 gtgatagagg cttgactgat ggagggactt gcagatagaa tctctagttt tgcgggttta
    48361 cagacagtac catctaaaaa gacaattccc ttttgaaagg cttggatatg gtcaggtgtt
    48421 agaagtccat taacctcaac ttgataagtc ttatcgacat gatattgggg atggaggagt
    48481 tggaagccca agggaccgtt atcggtcaag aggaggagtc ccgttgtatc tcggtccagt
    48541 cggccaacgg catagagctt gtcagactgg atgtcagatg gaagcaggtc catgacggtc
    48601 ggaagttcct tgtctttgtt ggctgtaacg acaccagcag gtttatgaag cataagatag
    48661 gtgtgttcat agccttgaat gattcgatcc tgaaaaagga gttcttgtag tcctgtatcg
    48721 atattttgag ctagggagcg ggctgggcaa ccatcgacta gaattttccc tctgaggagt
    48781 gcttgcttca tggcctttcg gctaactttt tcttgggcta ataaattatc taaacgcata
    48841 ctgtgcctat cttatcataa gtcgcttgct ttgaaaaggc ttgacgtgaa aagaaaagcg
    48901 gagtgaaaac atttacactt gcttgagtta tgttatttat ttgaaattat ggtataatcg
    48961 ttcagttaga aaataaattt tgaatattat agaggaaatc atgacaaaat taagagaaga
    49021 tatccgtaac attgcgatta tcgcccacgt tgaccacggt aaaacaaccc tggttgacga
    49081 attattgaaa caatcagaaa cgcttgatgc acgtactgaa ttggcagagc gtgctatgga
    49141 ctcaaacgat atcgaaaaag agcgtggaat caccatcctt gctaaaaata ctgccgttgc
    49201 ttacaacgga actcgtatca acattatgga cacaccagga cacgcggact tcggtggaga
    49261 agttgagcgt atcatgaaaa tggttgacgg tgttgtcttg gtcgtagatg cctatgaagg
    49321 aaccatgcca caaactcgtt tcgtattgaa aaaagccttg gaacaagacc ttgtcccaat
    49381 cgtggttgtt aacaaaatcg ataagccatc agctcgtcca gcagaagtag tggatgaagt
    49441 cttggaactt ttcatcgagc ttggtgcaga tgacgaccag cttgatttcc cagtggttta
    49501 tgcttcagcg atcaacggaa cttcttcatt gtcagatgat ccagctgacc aagaagcgac
    49561 tatggcacca atctttgaca cgattatcga ccatatccca gctccagtag ataactcaga
    49621 tgagcctttg cagttccaag tgtcactttt ggactacaat gacttcgttg gacgtatcgg
    49681 tatcggtcgt gtcttccgtg gtacagttaa ggttggggac caagttaccc tttctaaact
    49741 tgacggtaca actaaaaact tccgtgttac aaaactcttc ggtttctttg gtttggaacg
    49801 tcgtgaaatc caagaagcca aagcgggtga cttgattgcc gtttcaggta tggaagacat
    49861 ctttgtcggt gaaaccatca ctccgacaga tgcagtagaa gctcttccaa tcctacacat
    49921 cgatgagcca actcttcaaa tgactttctt ggtcaacaac tcaccatttg ctggtaaaga
    49981 aggtaaatgg gtaacttctc gtaaggtgga agaacgcttg caggcagaat tgcaaacaga
    50041 cgtttccctt cgtgttgacc caactgattc accagataaa tggactgttt caggacgtgg
    50101 agaattgcac ttgtcaatcc ttatcgaaac aatgcgtcgt gagggctatg aacttcaagt
    50161 atctcgtcca gaagttatcg taaaagaaat cgacggtgtt aaatgtgaac catttgaacg
    50221 tgtacaaatc gacactccag aagaatacca agggtctgtt atccaaagcc tttctgaacg
    50281 taaaggtgaa atgttggata tgatttcaac tggtaatggt caaactcgtt tggtcttcct
    50341 tgttccagcg cgtggtttga ttggatactc aactgagttc ttgtcaatga ctcgtggtta
    50401 cggtatcatg aaccatacct tcgaccaata cttgccattg attccagggg aaattggtgg
    50461 acgtcaccgt ggtgcccttg tttctatcga tgctggtaag gctacaactt actcaatcat
    50521 gtctatcgaa gaacgtggta cgatctttgt caacccaggt actgaggttt atgaaggaat
    50581 gatcatcggt gaaaactctc gtgaaaatga cttgacagtt aacatcacga aggcaaaaca
    50641 aatgaccaac gttcgttcag ctactaagga ccaaacagct gttatcaaga cgcctcgtat
    50701 cttgacactt gaagagtctc ttgagttctt gaacgacgat gagtacatgg aagtaacgcc
    50761 tgagtctatc cgtttgcgta aacaaatcct taacaaagca gagcgtgaga aagctaacaa
    50821 gaagaaaaaa tcagctgaat aagagctaga aagagagaaa gatggtctat ttagtcctag
    50881 gaattttact gctcctactc tatgtatttg cgacaccaga aagcattaaa gggactgtca
    50941 atatcgtcgc tatggtatgt attttagtgg cactcttgat tttattggtt ctatcttttc
    51001 tgaaaatttt tcaattacca acagaaatat tcctagcaat agccatgttg atcctagctt
    51061 actttagtgt gagagacatc acactcatgc cagtcaaaaa aagtaaaaga agataaaaaa
    51121 gagagtttcg gctctctttt tgctaggaat tttacaatag tttgaattgc tttttagtta
    51181 acaccatctc ttttacttgt atagtatgaa tgggaaaaac ttgaataaaa ttcgttaatg
    51241 gcatctattt tatatttggt tagtactggt tgcatattat tttgaaataa aactactata
    51301 taatattatt catagagata atatagtaaa acatctaaaa aattcgattt tttccttgac
    51361 aacaaataat aaaaatgtta ttcttatcac ataagaaagc gatttcaaca aaagagagag
    51421 gaggatttat tttgttgcat tgttgctcaa ggtgtgattt ttttgaaaga aaaaataaaa
    51481 tgaaaacgaa aaaacataga ttacttgctc tagctcttat ttcaaatttt acattattgg
    51541 gagctgcatc agctgctgta caatatccag atggaggagt atggacatat ggagaaggtt
    51601 caggaggtgg ttaggctttt tcaaattact atcatggtaa aaaatatcat tattcatcca
    51661 tcgtgagcag atgggatggt cattctgata aaggagaagc tcctgctgga aaaacctcct
    51721 atgcatggat ttggactaaa tggggagaac aagtagcatt ttactatgac tatgactaat
    51781 atttagtttt cttagataat tccccttgta agaaattaca gggggaattt tgtttacaag
    51841 gagtctacta tgaagaaact attcatatta ttatcaactt tttttctcag cttcttcctt
    51901 gcttggatta ttgtcttacg tgcgccacaa tatttatatg caagctatga ttccgtttct
    51961 ttacttcgtg tcaaaaaaga tactcaggaa ccgacgcgtg aggtatttga acaggaattg
    52021 gagaattttg caaactcaga acagagttta atagctagaa gaattgtaga gccgagtaag
    52081 gatgggacga ctcactttac ttatgcaact tatggtcagg gaactttacc aaaagaattc
    52141 caagaagcta gccaagaaag tcgtgaacgt agtgatccgc taaatagtta tctccttttg
    52201 tcaggctcct tgacgaaaga aaagcttgcc gataaattag gagatttggg ttataaagca
    52261 agtgctgacc gaaagatacc gccctatttt cttgcttttc gaatattact aaatcccctt
    52321 attttaatta gtttagcaat atttggctta tctttctttg ctttagtgat tatcactcgg
    52381 attaaggaaa tgagagcagc aggtataaaa ctcttttctg gtcagactct cttatccatc
    52441 atggggcatt ctttatcgac tgatatcaaa tggctccttc tatcagccct cctttccttc
    52501 ctaggtgggg gagtcgttct ttttagtcaa ggtttgtttt atcctatctt gttagccacc
    52561 tatggttttg ggattagttt ctatctgttg tttttattgg cgatttcaat tttactaatg
    52621 cttctttatc taatgagttt gaattacaaa gcattagttc ccgttattaa ggggagatta
    52681 ccccttaaac gcctgatgat tttaacccta ttgtgtcagt tagtagctgt ttttacagta
    52741 ggctacgctg ttaagacagg tttgacgtct taccaacgat tgaaagaact tgaaatttca
    52801 aaacaagcat ggcaggatag agcagactat tatcaaattt cttttggctt aggtgataga
    52861 ggaaaagata cagaaaatca gagcaagtgg tatgcctttg ccaaggaagc aatcgaagaa
    52921 gaacaagctc tttatgtaaa ggataatctg ctccattttg ccaatccaca aggaaaaaat
    52981 gaacagggag agacactgga tacctatagt ccagatgcta atacgctcta tgttagtccc
    53041 agttatttgg acaaggaaaa ggtcgtggta gatgctgaga ccaaacagaa gctagcccat
    53101 ctccaaaaag gtgagtttat cctcttgctc ccagaacatt tgcgctctcg agaagcagaa
    53161 cttaagaaag tttttgaaga aagattgagt tattatggaa aatctggtga ggaggcaagt
    53221 gctcctttgg attatgagat gaaagcgcac gttagttatc tttcaatggg agaaaagcgg
    53281 tttgtttata ataacggtga gaatcccgta tccactcagt atttgactga tccgatttta
    53341 gtggtattca cgccgacttc tacaggtgat agttttatat ccttatctag ttggtctatc
    53401 aatgctggaa aacaactctt tatcaaagga tatgagagtg ggctagaact cttgaagaaa
    53461 gctggaattt atgagcaagt atcctatctt aaagaaggaa gaagtgttta tctaactcgt
    53521 tataatgaag ttcaaactga aacagcaact ttaatcttag gagctattgt ggggatagct
    53581 agttccttgt tactatttta ttctgtcaat cttctatatt tcgagcaatt ccgccgagat
    53641 attttgatta aacgaatttc aggtttacga ttttttgaaa cacatgctca gtatatggtt
    53701 agccaatttg ccagttttgt atttggtgct agtttcttta ttttacgcag tagagactgg
    53761 gtaattggct tactcacttt attagtcttt ctagctagtg cagttttgac gctttaccgt
    53821 caagcacaga aagaatctcg tgtttctatg acaattatga aaggaaaata ggatgattga
    53881 actaaagaat atatctaaaa aatttggaag ccgtcagcta ttttcagata cgaatcttca
    53941 ttttgaaggt gggaaaattt atgccttaat cggtacaagt ggctgtggta agacaacact
    54001 cttgaatatg attggacgat tagagccata tgacaaaggg caaatcatct atgatggtac
    54061 ttctcttaag gacatcaagc cttctgtttt ctttagagat tacttaggat atttatttca
    54121 agattttggc ttaattgaaa gccaaactgt caaagagaat ctcgatctgg gtttagttgg
    54181 taaaaagttg aaggaaaaag agaaaatctc tttgatgaaa caagctctaa atcgtgtaaa
    54241 cctctcttat ttggatttga agcaacctat atttgagtta tcaggaggag aagcacaacg
    54301 tgttgcacta gcgaagataa ttttaaagga tccacctttg atcctcgcag atgaaccaac
    54361 cgcttcctta gaccctaaaa actctgagga attactttcc atcctagaat ctttaaaaaa
    54421 tccgaatcgg accattatta ttgcgaccca caatcctctg atttgggggc aagtggatca
    54481 ggtcattcga gttaccgatt tatcacatag atgatatggt aagattcagt tagaagaaag
    54541 agtcacaaac acactttgtg gcttttttat ttccataaaa atggtaaaat agtaggagta
    54601 gaaatggagt tcgagacatg aaagtaatag atcaatttaa aaataagaaa gttcttgttt
    54661 taggtttggc caagtctggt gaatctgcgg ctcgtttgtt ggacaagtta ggtgccattg
    54721 tgacagtaaa tgatgggaaa ccattcgagg acaatccagc tgcccaatgt ttgctggaag
    54781 aagggatcaa ggtcattaca ggtggccatc ctttggaact cttggatgaa gagtttgccc
    54841 tcatggtaaa aaatccaggt atcccctaca gcaatcccat gattgaaaag gctttggcca
    54901 agggaattcc agtcttgact gaggtggaat tggcttattt gatttcagaa gcaccgatta
    54961 ttggtatcac aggatcgaac ggtaagacaa ccacaacgac tatgattggg gaagttttga
    55021 ctgctgctgg gcaacatggt cttttatcag ggaatatcgg ctatcctgcc agtcaggttg
    55081 ctcaaatagc atcagataag gacacgcttg ttatggaact ttcttctttc caactcatgg
    55141 gtgttcaaga attccatcca gagattgcgg ttattaccaa cctcatgcca actcatatcg
    55201 actaccatgg gtcattttcg gaatatgtag cagccaagtg gaatatccag aacaagatga
    55261 cagcagctga tttccttgtc ttgaacttta atcaagactt ggcaaaagac ttgacttcca
    55321 agacagaagc cactgttgta ccattttcaa cacttgaaaa ggttgatgga gcttatctgg
    55381 aagatggtca actctacttc cgtggtgaag tagtcatggc agcgaatgaa atcggtgttc
    55441 caggtagcca caatgtggaa aatgcccttg cgactattgc tgtagccaag cttcgtggtg
    55501 tggacaatca aaccatcaag gaaactcttt cagccttcgg tggtgtcaaa caccgtctcc
    55561 agtttgtgga tgacatcaag ggtgttaaat tctataacga cagtaaatca actaatatct
    55621 tggctactca aaaagccttg tcaggatttg acaacagcaa ggtcgtcttg attgcaggtg
    55681 gtttggaccg tggcaatgag tttgacgaat tggtgccaga cattactgga ctcaagaaga
    55741 tggtcatcct gggtcaatct gcagaacgtg tcaaacgggc agcagacaag gctggtgtcg
    55801 cttatgtgga ggcgacagat attgcagatg cgacccgcaa ggcctatgaa cttgcgactc
    55861 aaggagatgt ggttcttctt agtcctgcca atgccagctg ggatatgtat gctaactttg
    55921 aagtacgtgg cgacctcttt atcgacacag tagcggagtt aaaagaataa tatgaaaaaa
    55981 attgtcttta caggtggggg gacggttgga cacgttaccc tcaatctttt gttaatgcct
    56041 aagttcatcg aagatggttg ggaagtccac tatatcgggg acaagcgtgg tatcgaacac
    56101 caagaaatcc ttaagtcagg tttggatgtc accttccatt ctattgcgac tggaaaattg
    56161 cgtcgctatt tctcttggca aaatatgctg gatgtcttca aagttggttg gggaattgtc
    56221 caatcgctct ttatcatgtt gcgactgcgt ccacaggctc ttttttcaaa gggaggattt
    56281 gtctcagtac cgcctgttat cgctgcgtgt gtgtcaggag tgcctgtctt tattcacgaa
    56341 tctgacctgt ctatgggctt ggccaataaa atcgcctata aatttgcgac taagatgtat
    56401 tcaacctttg agcaagcttc gagtttgtct aaggttgagc atgtgggagc agtgaccaag
    56461 gtttcggatc aaaaaaatcc agaaccagat gaattggtgg atattcaaag ccactttaat
    56521 cataaattgc cgactgtatt gtttgttggc ggttctgcag gtgctcgtgt ctttaaccaa
    56581 ttggtgacag accataagaa agaactaaca gagcgctaca atattatcaa tctaactgga
    56641 gattctagcc tgaatgagtt gagccaaaat ctttttcgtg ttgactatgt gaccgatctc
    56701 tatcaaccct tgatggaatt ggctgatatt gttgtgacac gtggtggtgc caatacgatt
    56761 tttgagctct tggcgatagc aaaattgcat gtcattgtgc cacttggtcg tgaagctagt
    56821 cgtggtgacc agattgaaaa tgcagcttac tttgttaaga aaggctatgc agaagacctt
    56881 caagaaagcg atttgacctt ggatagtttg gaagagaagc tttctcactt actaagtcac
    56941 aaggaagatt accaagctaa gatgaaagct tctaaggaat tgaaatctct agcagatttt
    57001 tatcaattgt tgaaaaaaga tttatcataa ggaaagtaaa tgtcaaaaga taagaaaaat
    57061 gaggacaaag aaatcctcga agaattgaaa gagttatcag aatggcagaa acgaaaccaa
    57121 gaatatctaa aaaagaaggc tgaagaagaa gcggctctag ctgaggagaa ggaaaaggaa
    57181 agacaagctc gaatgggaga agaatctgag aagtcagagg acaaacagga ccaggagagt
    57241 gaaacagacc aggaagattc agaatcagct aaggaagagt ctgaagaaaa agtagcatcc
    57301 tcagaggctg acaaagagaa agaagagaaa gaagaaccag agtctaaaga gaaggaggaa
    57361 caggataaaa agcttgctaa aaaggctaca aaggaaaaac cagccaaagc aaagattcct
    57421 ggtatccata tcttgcgagc cttcacgatt ttatttccaa gtctgctttt attgattgtc
    57481 tctgcctact tgctcagtcc ttatgcgacc atgaaagata ttcgtgttga gggaacggtg
    57541 caaactacag ctgatgatat tcgacaggct tcaggcattc aggattcgga ttatacgatt
    57601 aaccttctgc tagacaaggc aaaatatgaa aagcagatta agtctaacta ttgggttgaa
    57661 tcagctcaac ttgtctatca atttccaact aagttcacta ttaaggtcaa ggaatatgat
    57721 attgtggcct actatatttc tggtgaaaat cattatccta ttctttccag tggtcagctt
    57781 gagactagtt ctgtgagtct gaacagttta ccagaaactt atatatcagt tctctttaat
    57841 gacagcgaac aaatcaaagc ctttacgtca gaacttgctc aaattaaccc agaactcaag
    57901 gtggctatcc aaaaggtgga attagcccca agcaaggtga cttccgattt aattcgattg
    57961 actatgaatg attcggacga agtcttggtt ccgctttctg aaatgagtaa gaaattgcct
    58021 tattacagta agattaagcc tcaattgtca gaaccaagtg tggtcgatat ggaagctgga
    58081 gtctacagct acactgtagc ggataaattg attatggaag cagaagaaaa agctaaaaaa
    58141 gaggccgaag aagctgagaa aaggcaggaa gaggaacgta aacgtttaga agaagaacag
    58201 aaaaaacaag aggaagagag caatcgaaac caaacgaccc agcgctcatc gcgtcgctag
    58261 gttttacctt ttctcctata gctctttagt tgccatgttt ttacgtttaa tatttgacat
    58321 ctgtttttct ttatgttata tttgcaatgt aatcgattac aaaaaaattt tttatgaaga
    58381 aggtaacaca tatgtcagat gaagtattct tatttgaaga aattgaagaa atcgtagccc
    58441 ctacagatgg tgaattttgg gggggagttt tacttggaac tggggtagtt ctcttaattg
    58501 gagtagcctg ttgttaaaaa gatagggagt gataatcatg caagataact ttttatttga
    58561 ggaaattgaa gaaatttcag taccagttag tgatttttca gctggacttg caacaggtat
    58621 cggattaggt ttagcaatcc ttgctcttgc tggttgttga agtttgttca cttactaata
    58681 tcaagctttt tcaatttcat tttagacagt catttgaatt ttccgtatta gtcttgcagc
    58741 aagagattaa tagaattagt cattatttta ttgattgcgg actgagggac tagagtatgt
    58801 tttacttagc ccctctttta tttattaaag gttaggtttg ttatgagaat tgttgataag
    58861 attaagatac tacctactcc ttatgaggga cagtatcatc tatatatacc atccagtaag
    58921 aaacatgtat tagttgggaa acaggaaaaa aaatggttag agcaactaat aggtcaagaa
    58981 tttaccatat cggacttatt agtgttagta gggaagaaat attatttaag attttatatt
    59041 gttc
//
LOCUS       DAVKLX010000007        52081 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKLX010000007 DAVKLX010000000
VERSION     DAVKLX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 52081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.007"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..2724)
                     /locus_tag="VW325_000459"
     CDS             complement(<1..2724)
                     /locus_tag="VW325_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEU9733918.1"
                     /translation="MDALTRRQFDRAMFAKNRTLAIRVGDYASRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFGISAMRY
                     HIEQVPEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNITINADSYFLHGL
                     TKSEIEYQIAGITCKTDKKSLTVGMKTGRSLELDNVFMTQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFTFKGGLRGRISADSKAGNDTQYS
                     YEGTITKHIKQQGGIEAKIQAQIEATDKDFDQKVDKIKKDFNDQVELAKARAEEVKRE
                     LSDTINQRFNSFDNGPLKETKRKAEEALRNAGASSLLAQEAKRIGLDSVARLEAFKSQ
                     TTSAQTALSGDLDALKRTIVNDIRPKQAQVEAEIAKQVEALVQTKKELAGASTLLAQE
                     AKRIELDSVARLEAFKSQTTSAQTALSGDLDVLKRTIANDIRPKQAQAEAEIAKQVEV
                     LSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEELASRIASVQA
                     GSSRNYFRNSRSRTFTTGGQAVYDYRTFIVPDFWKNSDRFKRDYVRISFDVTFPVALA
                     NDMPAMVHFSAHPWYAYRNLIFKGGTVERQHFEFTIDLSSSSEDYQTNNVFIRFGTNY
                     GFPAGLQVVIENAMLSVGNYFPAYQPAYEDQEDRVSVVESNFKQRADSLDAGVSRLTE
                     GLRTKADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATK
                     DKASKSELTQTAEELSSKIASVQASGRNLFLNSLFKQDISKTGIWTTSTYTA"
     gene            complement(2729..3079)
                     /locus_tag="VW325_000460"
     CDS             complement(2729..3079)
                     /locus_tag="VW325_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733919.1"
                     /translation="MAQLTINGVAVKPPKSFKVGIQDIDGETGRNANGDMVRDRITTK
                     RKLDCEWGMMTQGEISQLLHAVSSEFFEVSYPDPMDGQVTKTFYVGDRTAPSYTFTEK
                     FKPWSGAKFNLVER"
     gene            complement(3088..6693)
                     /locus_tag="VW325_000461"
     CDS             complement(3088..6693)
                     /locus_tag="VW325_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733920.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSNSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEAARYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIKSTEAFKRFSNGQSWDQLDFQTQQQ
                     IRLMAILEQATAKYGDTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIKRIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSIATIGLGIGVFLAESIANG
                     LGRQKERIIKALVALFDNIGNIAEAVGNIAQDFSSAFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKDFEKVVVTNAPKISSIFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVAPAINSIANAFNGLIDIIQILWEGSWKPFAEFLSNTFGISIETVADL
                     LGGIILEALKLLADTIKLVADGFTAFSDWCKENKEIISTIASVIGTLATVWQGIKFLS
                     WAEQAGGLAGAFELLSSKVSFIVSGIKNLGLALKALTFDKLVSFGETIYLNALYAKDF
                     VVNSGKTIAQLGKTALELGKSALAWTAHTAKMGLATAAEFAHSVAAGVATAATWAFNA
                     ALAVLTSPITLVIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICRAIGEF
                     FSGLWTKLQEIFEPIGQWFGEKFQQAWDAIVNIFTPIGSWFGQRWADVTSALANIGAW
                     FTDMFQKAWTGLTNIFSKLGLWFGERWADVTSVLANVSSWFGNMFTSAYNAVKNAFSS
                     IGGFFSGVWSTVQSIFVNAGQKVGSAVGGAFRSAVNGVLGTIENVVNGFIGMINGVIG
                     MINKIPGVSLGGIGYVSLPRLARGGIVDSPTIAMIGEAGKEAVVPLENTGFIQTLGRV
                     VSSAVVNAMAGISPQGGFSGDGDIVIQIAGHEFGRVAIQEINKEHERAGQTLLKI"
     gene            complement(6680..7030)
                     /locus_tag="VW325_000462"
     CDS             complement(6680..7030)
                     /locus_tag="VW325_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733921.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYR
                     LSQMISNHVSLLLSKDAKVFEFWEYAPELFVEEQQAVEQERQRQALLLHKERMRDFAE
                     RHNRKRKEEVNGNS"
     gene            complement(7069..7449)
                     /locus_tag="VW325_000463"
     CDS             complement(7069..7449)
                     /locus_tag="VW325_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733922.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSLEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVVQ"
     gene            complement(7454..7867)
                     /locus_tag="VW325_000464"
     CDS             complement(7454..7867)
                     /locus_tag="VW325_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9733923.1"
                     /translation="MLANGITLSYSKTKGSYTKLVGLKEVPEFGIELEKVENTTLEDT
                     VKKYELGIGDVGELEYKFSYNNSSATAPYRVLRKAADDKEKLYFEQAYPDGTKVTFEG
                     QVSVKLGGGGVNAVIDFTLKIALQSELEFADGVGG"
     gene            complement(7873..8241)
                     /locus_tag="VW325_000465"
     CDS             complement(7873..8241)
                     /locus_tag="VW325_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733924.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDKEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGVVDLDSELVFQFRMEN"
     gene            complement(8238..8753)
                     /locus_tag="VW325_000466"
     CDS             complement(8238..8753)
                     /locus_tag="VW325_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEU9733925.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNAAIKVVQADAR
                     RLVPVNDGELITSIKTRAKMDGDRAIGEVYTNLKYAPYVEFGTGPKGQASHSGISPEV
                     SVTYKSHPWYVHEDQINVGSYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIQ"
     gene            complement(8728..9066)
                     /locus_tag="VW325_000467"
     CDS             complement(8728..9066)
                     /locus_tag="VW325_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733926.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYAKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            complement(9047..9358)
                     /locus_tag="VW325_000468"
     CDS             complement(9047..9358)
                     /locus_tag="VW325_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEU9733927.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGEHSRTEGGIAVVYGENGLSTGLLQRIRMHRLARVAGHVFEA
                     E"
     gene            complement(9360..9548)
                     /locus_tag="VW325_000469"
     CDS             complement(9360..9548)
                     /locus_tag="VW325_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733928.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGEELEITEERYAELADNIASNGVT
                     ISDVLEKILPEPEFLEED"
     gene            complement(9538..9720)
                     /locus_tag="VW325_000470"
     CDS             complement(9538..9720)
                     /locus_tag="VW325_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEU9733929.1"
                     /translation="MSLLLRRHYIQEEQVNQYSDLENKTLEELKDLAKEAGVAGAYKL
                     TKAEIVEVLEELKSEI"
     gene            complement(9732..10577)
                     /locus_tag="VW325_000471"
     CDS             complement(9732..10577)
                     /locus_tag="VW325_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HEU9733930.1"
                     /translation="MTAGQTKLATMVNPEVMADMVAAKLPKLIKFTPLAYVETKLEGQ
                     PGSTLTVPAWEYAGDATEIEEGQAITPDQLTTKKTTMTIKKAGKGYEITDESLLSGLG
                     DPLGQATYQLGLAIANKIDNDLVAVAKTATQHITETPTTLEAIDKALDIFEDEEDAQY
                     VAIINPKDATKLKTAVAKEWIKGSELGANMVVSGTFGEVDGVQIVRSKKVDEGKGFLV
                     KVSPSQTQTDDANKYGAFVILLKRDVAIETDRDILKKTTVITGDEHYGVYLYDPTRVV
                     KFGGA"
     gene            complement(10584..11168)
                     /locus_tag="VW325_000472"
     CDS             complement(10584..11168)
                     /locus_tag="VW325_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEU9733931.1"
                     /translation="MSEEINATVSTESTETVDTQGNVDSVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTSAETVSQSVAVLQKSWEQAVQKGVQEKLKGGAPMKQAPV
                     DSDGITKEEFARMGYQSRNELYQKNPELYKKLKG"
     gene            complement(11385..11636)
                     /locus_tag="VW325_000473"
     CDS             complement(11385..11636)
                     /locus_tag="VW325_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733932.1"
                     /translation="MLEKAKQLASQEFSRLSGREIKAEDCFVVWFSKTLQNWKALVST
                     NAITSSEPCGNYAEITHNGDKKETYVDVYAKVSNRAIKD"
     gene            complement(11638..11883)
                     /locus_tag="VW325_000474"
     CDS             complement(11638..11883)
                     /locus_tag="VW325_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733933.1"
                     /translation="MKYRKKPVVVEAVQWNGNNHKEVIDFAENKIWFDALGNIWIATL
                     EGDMVAKKGDYIIKGVQGEYYPCKLDIFAETYEKTEE"
     gene            complement(11926..12339)
                     /locus_tag="VW325_000475"
     CDS             complement(11926..12339)
                     /locus_tag="VW325_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9733934.1"
                     /translation="MIDIALAIAKKAHAGQVDKAGVDYIQHPLYVASQVNTEQEKAVA
                     LLHDVIEDSDITAADLFASGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIHYLST"
     gene            complement(12336..12545)
                     /locus_tag="VW325_000476"
     CDS             complement(12336..12545)
                     /locus_tag="VW325_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733935.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSMDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            complement(12547..14184)
                     /locus_tag="VW325_000477"
     CDS             complement(12547..14184)
                     /locus_tag="VW325_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEU9733936.1"
                     /translation="MKNQEYWAKRKANLIYQQMDKAEKQADQFDKVYQEAKTYLDKEI
                     NKIFDKFQRDYGLSQVEARQVLKNMKDKKNLNELRKVLEARPNDPNIQRLLADLDSPA
                     YSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDLMKDSYYKATFDLQQQTGLAYG
                     FSGLPENEIKHLQSFSWVGDGSTYSTDIWKNTGKLTSSIKDELLISLMTGRDTRETAQ
                     AIAERFNVGQNDARRLVRTESAFFHNQMELLSYEEADIEKYIFVAVLDKRTSRICQEH
                     DNQVYDRDKAVPGVNCPPMHPWCRSTTVGYDEDADYSKLKRRARNPETGKVEYVPADM
                     TYKEWYSKYVAKDGEKVYNQDTREAKAKFYSEQLLSKISGVEPKITSDMQRIAGENKL
                     AGLEFRKKTVESLSRKITTDSQAENISLSKATSKINDALRYTTIFDPDTFAKEYLKMK
                     QKLIAEGYKIVKVKNTWLVDGPYKGVNTVVEKDGINFEMQYHTQESFDLKNDSLHELY
                     EKYRDTNTSDLERMKLFKEMLDLSNGLEIPKNIERVK"
     gene            complement(14093..15562)
                     /locus_tag="VW325_000478"
     CDS             complement(14093..15562)
                     /locus_tag="VW325_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU9733937.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHNTESSHLKKLKSYYM
                     GEHAILKHTRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMTELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNKD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRPVR"
     gene            complement(15574..16872)
                     /locus_tag="VW325_000479"
     CDS             complement(15574..16872)
                     /locus_tag="VW325_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEU9733938.1"
                     /translation="MSQTYQIKQNDIVVDLPKTVGAGYGQFWRSRSLYRVVKGSRGSK
                     KSKTTALNYVVRLLKYSWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITIKATGQKILFRGLDDELKITSITVDVGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETRRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFNKDELLQDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAELRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            complement(16850..17290)
                     /locus_tag="VW325_000480"
     CDS             complement(16850..17290)
                     /locus_tag="VW325_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEU9733939.1"
                     /translation="MSGLRIKQKRFADEYIISGNATEAYKKAGYRVSSDRVAGVEGHK
                     LLKNPKIKSYIDERLKQLDSEKIADQQEVLGYLTSVMRGETQEQTLISIGELGQTITD
                     IDVGAKDRIKAAELLGKRHRLWTDKVEADVSGTVVFANESDIPD"
     gene            complement(17595..17677)
                     /locus_tag="VW325_000481"
     tRNA            complement(17595..17677)
                     /locus_tag="VW325_000481"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17644..17646),aa:Tyr,seq:gta)
     gene            complement(17758..18162)
                     /locus_tag="VW325_000482"
     CDS             complement(17758..18162)
                     /locus_tag="VW325_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU9733940.1"
                     /translation="MNKAKELLKELQDLDMDIQSRIDEINELEAGLLSSPKWSEVKVQ
                     GGQTRKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRMINRLKNPKYRAVLRMTYINK
                     GTADSVCYDLNMSRTTYYRLKNEAVLALEEVI"
     gene            complement(18235..18606)
                     /locus_tag="VW325_000483"
     CDS             complement(18235..18606)
                     /locus_tag="VW325_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733941.1"
                     /translation="MRPCKYPYSGRRKKQETPSPIFSARPIFDEVPIVEEVKVEFGVE
                     ASMGRIYPETLIHLDISGYGNRVHSVHRFPGILLSVGESIQLKMLFYKRLRNFTTDRF
                     LTFRESDWKFFIRDLVNEFKH"
     gene            complement(18603..19040)
                     /locus_tag="VW325_000484"
     CDS             complement(18603..19040)
                     /locus_tag="VW325_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733942.1"
                     /translation="MTPRYRAWIKTEKRMFFSDDILAIDYENKEIVTQQVYFENGLPD
                     DRDIYCYDFDEIELMQSTGLKDKNGKEIFEGDIVRTTRFLGRADEIGGFYEYDKEFIG
                     IVKQLEGSWVIDTGSDAVCLWTEIEENEIIGNIYENKEFGGRK"
     gene            complement(19052..19474)
                     /locus_tag="VW325_000485"
     CDS             complement(19052..19474)
                     /locus_tag="VW325_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733943.1"
                     /translation="MTETIKLPDYYEPDWKNARYGSLEELKELLLFKRIVKWDKDFLL
                     LEDGTKVTIEMSESDCCASAGGEFQDVSLDAVITNIEIGEPEEIPDHWGTGYKNKVTI
                     FHNQNPVAIANCEAEHNGYYYSVCSLVIGDIHFPVVNA"
     gene            complement(19471..19731)
                     /locus_tag="VW325_000486"
     CDS             complement(19471..19731)
                     /locus_tag="VW325_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEU9733944.1"
                     /translation="MKRFIAIWILLSAGLNIWQSIYIKKLEEKRPIVVYKADNAGAEI
                     FGKVVEKGRHGKLYTLTIRDYGVFVVTKDVYDKVKVGDEVLL"
     gene            complement(19889..20377)
                     /locus_tag="VW325_000487"
     CDS             complement(19889..20377)
                     /locus_tag="VW325_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU9733945.1"
                     /translation="MNKQELIEKYKKLEGVWNAEGAELARQIFLQDLEQLDKPKPVKV
                     PQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPEWFYENNIEKFCLAWLDGYEVEKE
                     KRYLVTLKNRQPLVKSQSGSTLYFSQDITARNYKGTQKELEDANFGWVFDCPGIEIEE
                     VE"
     gene            complement(20379..20696)
                     /locus_tag="VW325_000488"
     CDS             complement(20379..20696)
                     /locus_tag="VW325_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733946.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWIGYSPSMAQVVIAVYLIY
                     GGN"
     gene            complement(20721..20903)
                     /locus_tag="VW325_000489"
     CDS             complement(20721..20903)
                     /locus_tag="VW325_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733947.1"
                     /translation="MADYALYQGDVFVTLGTLAQISSETGITERMLKYYTYTSHQRRH
                     PNGRAVIKIEEEDNEN"
     gene            complement(20919..21350)
                     /locus_tag="VW325_000490"
     CDS             complement(20919..21350)
                     /locus_tag="VW325_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEU9733948.1"
                     /translation="MKLTLNIEPKPQSRPRFARRGSFTTTYEDKDMKTWRNHCQLLIA
                     NQYMGQPILEGALRARLRFYIKPPQYISKVKKNQQALLDEIIPVGKKPDIDNYEKALY
                     DSMSGIVFQDDGQIALHDVGKFYSLNPRIEVEIEVMKSLSI"
     gene            complement(21347..21676)
                     /locus_tag="VW325_000491"
     CDS             complement(21347..21676)
                     /locus_tag="VW325_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733949.1"
                     /translation="MASKINATERIAIIIDKQKIEVVTTLNYDMSISFDNKDTAPTLD
                     DNGDLFEPVYKCKVKAIPKNDVFFTSLTRVKSNIKTLQEVKKFFEFVNENRENLFEMA
                     GFKGALE"
     gene            complement(21690..21899)
                     /locus_tag="VW325_000492"
     CDS             complement(21690..21899)
                     /locus_tag="VW325_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733950.1"
                     /translation="MEWTDWVDWKPETKTDIKTKIENDGYTFPHYDKKNNGVKYVIST
                     LDIKRDCLRLGVPFEDVYPLQTTLF"
     gene            complement(21901..22596)
                     /locus_tag="VW325_000493"
     CDS             complement(21901..22596)
                     /locus_tag="VW325_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9733951.1"
                     /translation="MEIDKIIKKDVLEFMETIPDNKIDLIVTDPPYLINYKTNWRKEK
                     HKFSNVIKNDNNPELIKEYIKECYRILKDDTAIYIFCSFDKVDFFKKEIEKYFSVKNI
                     IIWRKNNHTAGDLEAQFGKQYEMIILANKGRKKFNGERLTDVWDFKRVSSDKLLHQNQ
                     KPIELIKRCIVKHSDVGDTVFDGFMGSGTTALAALETDRHFIGTEIDEYYFGIAEERI
                     KNHNAQLSLFDEV"
     gene            complement(22609..23025)
                     /gene="ssb"
                     /locus_tag="VW325_000494"
     CDS             complement(22609..23025)
                     /gene="ssb"
                     /locus_tag="VW325_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9733952.1"
                     /translation="MINNVTLVGRLVAPPDLRKTPNNVSSLQGTLAVNRNFKNENGER
                     EADFINFQAWRGTADIIAQYCSKGSLIGLTGRLQVRSYEKDGQRRYVTEVIAESVALL
                     ESRNSQHGQGNSFQNGNSSPFTDPNPFDLPNDGLPF"
     gene            complement(23120..23455)
                     /locus_tag="VW325_000495"
     CDS             complement(23120..23455)
                     /locus_tag="VW325_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HEU9733953.1"
                     /translation="MIKTVFFSCDYPHHEAIDDQINSWLAENPGIKLIDIKFQSNVSA
                     VADSGVSAEYWHTSALIIYKVPSENNISSINSNGLGFIISCEKCGSLSIIKGKDVGQN
                     VCYECKGEK"
     gene            complement(23448..23771)
                     /locus_tag="VW325_000496"
     CDS             complement(23448..23771)
                     /locus_tag="VW325_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733954.1"
                     /translation="MDIRKISDSVAIYSDGKKLQVIHNLGDEFILDFNVGEDSVWNLN
                     GQVVEIIDMIEPVFKVFSFCSKSGEGMQRLKHAIVHFEIFEQYIRDNQEDLMIWWHNP
                     GGEYD"
     gene            complement(23939..24979)
                     /locus_tag="VW325_000497"
     CDS             complement(23939..24979)
                     /locus_tag="VW325_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEU9733955.1"
                     /translation="MQELQVKVTQAQVEIIDREKFEQNINEVVAKYQNYTVTAATIKD
                     DKQTLADLRKLDKQVSDERIRNKKVLSEPADEFDKYVKNAIQPLKDIITKIAGDVKEF
                     EEHQKAVRIDTVKGYLANKSAEYMLDPRLFDEKALEYVKASDFMADGVTLKKATMKSL
                     DDMVTFEFQKQQEFEKAKSAISGLCAEYGMTDSPYIRQLKDLTLAEVFEQIKTDYEFE
                     KQKEELRQDLERAERSNQELLAAQQTKQQEQAPKSTETPNFDPETGKILDGGQILQNE
                     PNALRGAENDLKRYAQKMTLEVYFVDTAEKDCFKAGLSKLGFDFKKNYQVSGYQRIDP
                     LTQAELNEQCGW"
     gene            complement(24989..25753)
                     /gene="bet"
                     /locus_tag="VW325_000498"
     CDS             complement(24989..25753)
                     /gene="bet"
                     /locus_tag="VW325_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEU9733956.1"
                     /translation="MTNNQLATQIKRDITTDPSLLTGADIKKYFDPQNLLTEKQVGQA
                     LALCKGRNLNPFANEVYIVAYQNNSGTEFSLIVSKEAFMKRAERCEGYDGFEAGITVM
                     RNGEMIEIEGSLKLPEDILIGGWAVVYRKDRSHRYKVTVDFNEYVKTDRNGNPRSTWK
                     SMPATMIRKTALVQTLREAFPDELGNMYTDIDGGDTFDAIKDVTPQESREDVVARKMA
                     QIDQFNKEQEANHADPEPTQNEDPIQGELLDGELEY"
     gene            complement(25765..25995)
                     /locus_tag="VW325_000499"
     CDS             complement(25765..25995)
                     /locus_tag="VW325_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733957.1"
                     /translation="MTQMTNKGKSFIRAEISEKQKEYIRLLAELRGVTTQELLGQVVE
                     RFIDRNLQLIQDYKNELDDLNSKSRRRIDMNT"
     gene            complement(26115..26258)
                     /locus_tag="VW325_000500"
     CDS             complement(26115..26258)
                     /locus_tag="VW325_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733958.1"
                     /translation="MTKIEIENRVWLLANHEEKNELLDLGLTSKARYVKRVLELGKVY
                     AHV"
     gene            complement(26245..26505)
                     /locus_tag="VW325_000501"
     CDS             complement(26245..26505)
                     /locus_tag="VW325_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733959.1"
                     /translation="MFKALKTIKKIKQLQKEMHDVSLAFLALQDVGLMPETERSKAKA
                     QTMHDVSHMLKDVLGGKSVDEAMKRLNSEVKIEEVEQEDDKD"
     gene            complement(26518..26772)
                     /locus_tag="VW325_000502"
     CDS             complement(26518..26772)
                     /locus_tag="VW325_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733960.1"
                     /translation="MYIISIYVKNTETGNEDFSLIGRDFLPTGHQDYIARVFETKEEA
                     IDYLKSISYIASGVHGNDWVYQNEKLPEIESRCRIWKVGE"
     gene            complement(26773..26994)
                     /locus_tag="VW325_000503"
     CDS             complement(26773..26994)
                     /locus_tag="VW325_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733961.1"
                     /translation="MKLLTKLKLRLEVVLKAVNLDWREVAVELMTDLFEERKRRFAFE
                     QENYDLKQELAAYKYKENFDIKARLQGEV"
     gene            complement(26994..27155)
                     /locus_tag="VW325_000504"
     CDS             complement(26994..27155)
                     /locus_tag="VW325_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733962.1"
                     /translation="MKRKKEQWKPRIVNIMADGSQVDDLTGYVIPAGHSYYDIILGMN
                     KQSNEEGVA"
     gene            complement(27220..27363)
                     /locus_tag="VW325_000505"
     CDS             complement(27220..27363)
                     /locus_tag="VW325_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733963.1"
                     /translation="MEDKIIELADYFISESTTYREAKIACEKLLKQVSHEIELRAMES
                     KTV"
     gene            27481..27705
                     /locus_tag="VW325_000506"
     CDS             27481..27705
                     /locus_tag="VW325_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015990842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733964.1"
                     /translation="MSKLSRKPNHHVKKLTWSDLDNILISAFTESATDHPSAVIRLSD
                     YEMSKSEIIEEAIAQGYTVIDNSDDFLEFE"
     gene            complement(28016..28282)
                     /locus_tag="VW325_000507"
     CDS             complement(28016..28282)
                     /locus_tag="VW325_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9733965.1"
                     /translation="MMVVLANHPNWQVYPDEIAKRKGVSRDTVDSYFKILEKNGYLRI
                     VKKGMGRGKGVRVFRFFSDVKISDFQFEIMKQRLNESISKLSTG"
     gene            complement(28447..28635)
                     /locus_tag="VW325_000508"
     CDS             complement(28447..28635)
                     /locus_tag="VW325_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9733966.1"
                     /translation="MQGERLRKWREKEKMSQEELAEKSNVSRTTIHLIESGQSSTVKI
                     RTLQKLAVVFNKQVKDFF"
     gene            complement(28688..28849)
                     /locus_tag="VW325_000509"
     CDS             complement(28688..28849)
                     /locus_tag="VW325_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733967.1"
                     /translation="MRPIGYRLNVEVSGIEELKEACKEVSKKAEELQEAIDRLSIIEF
                     ELKAKPVND"
     gene            28896..29702
                     /locus_tag="VW325_000510"
     CDS             28896..29702
                     /locus_tag="VW325_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEU9733968.1"
                     /translation="MLSIDTQFVDTISKILSDYVSHSEITRMGEVLGYPQNDQNSGLN
                     KHHRVHNIMSDILNKTQDKSNIKLVIEYICNPLRYIDTVSDFENLRLKLNVVLSLKGL
                     TISDDGHVVITTASQTLVEAKKRFESLEHMLRTLNVHPNVLKFCTQELLQENYFHAVF
                     EASKGIFHRIRLLTGSSLDSASLIDQCFKTKEPIMIINGNKLQTLDEQSEYKGLKNLL
                     LTIAHLYRNSKAHKLKYYNPDSVHDALTALTLMSLAHNLLDNCTNTRRLD"
     gene            complement(29637..29930)
                     /locus_tag="VW325_000511"
     CDS             complement(29637..29930)
                     /locus_tag="VW325_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009853499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733969.1"
                     /translation="MEKLAEVTQEETEKILTYDEIVLLKPITHLHKSIFRQTEYLSEQ
                     LTERLHHLEDYNSPIDEETIRLAEVTIEFYNLLIKSPSISTVVKEIVSEGHKS"
     gene            30173..30622
                     /locus_tag="VW325_000512"
     CDS             30173..30622
                     /locus_tag="VW325_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733970.1"
                     /translation="MNNFSFIETLAIAAIPAFVSGMWSYIAAKGNSKHEIDKINIAHS
                     QELENVENQFKQDMEKMQKQHSQELYSLQQTHELRLLELEKVSQLDTQTDQGLKINDL
                     IFKAISGEISADVAIKNISTLSHFANKQQPSDLQKQFMKKLSKKNHK"
     gene            complement(30613..30780)
                     /locus_tag="VW325_000513"
     CDS             complement(30613..30780)
                     /locus_tag="VW325_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733971.1"
                     /translation="MNESFLTILGISMIASFITNLIAYLAGKHHLKKKIKNHKMWFDS
                     EIERIKKKYHL"
     gene            complement(30855..31130)
                     /locus_tag="VW325_000514"
     CDS             complement(30855..31130)
                     /locus_tag="VW325_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733972.1"
                     /translation="MNTLNEKAINIFKAVAKETLIQGTYEENFLYSQLETFSTNCRQF
                     TFGWTELADEIERQERYLLDSGFTQEEIDDIRFDAAFAGMLDKMNVA"
     gene            31285..32073
                     /locus_tag="VW325_000515"
     CDS             31285..32073
                     /locus_tag="VW325_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9733973.1"
                     /translation="MKLGELLKSYRTEHKLSMDAFCELSDLTKGYISMLEKNEHPKSK
                     KPIVPSYDTIEKIAKGMQISTEDLIDMLDDDQEIQINATPALLSKSPIQTIYDELEPP
                     RQGKVLNYAKRQLDEQENEEETKINEVSENIIRLDDYRQTTYRRVTGVVSAGSGSIQD
                     DDLDMEVSFYEDEIPDDYDAIAYVVGNSMEPKIKNGDYLFIKNTQQVDYNTIGIFQVD
                     GANYVKKLRQGYLESLNPDYEDIHLDESNDIRTIGEVVSVYREK"
     gene            32075..32275
                     /locus_tag="VW325_000516"
     CDS             32075..32275
                     /locus_tag="VW325_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733974.1"
                     /translation="MSNESRPMEVIKHNLDCKCHRRREWIRVNDKWHAIEFSVDDPNE
                     PPMTEEEKANVALILQQHLSKE"
     gene            32457..33902
                     /locus_tag="VW325_000517"
     CDS             32457..33902
                     /locus_tag="VW325_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9733975.1"
                     /translation="MITTNKVAIYVRVSTTNQVEEGYSIDEQKDKLSSYCDIKDWNVY
                     KVYTDGGFSGSNTDRPALESLIKDAKKRKFDTVLVYKLDRLSRSQKDTLHLIEDVFIK
                     NGIEFLSLQENFDTSTPFGKAMIGLLSVFAQLEREQIKERMQLGKLGRAKSGKSMMWA
                     KTSYGYDYHKETGTVTINPAQALTIKFIFESYLRGRSITKLRDDLNEKYPKHVPWSYR
                     AVRTILDNPVYCGFNQYKGEIYPGNHEPIISKEEYDKTQSELKIRQRTAAENVNPRPF
                     QAKYILSGIAQCGYCGAPLKIMLGVKRKDGSRLKKYECHQRHPRTLRGVTTYNDNKKC
                     DSGFYYKDKLEASVLKEISKLQDDADYLDKIFSGDNAETIDRESYKKQIEELSKKLSR
                     LNDLYIDDRITLEELQSKSAEFISMRATLETELENDPALRKNKRKADMRKLLNAEKVF
                     SMDYESQKVLVRRLINKVKVTAEDIVINWKI"
     gene            complement(33920..33982)
                     /locus_tag="VW325_000518"
                     /pseudo
     CDS             complement(33920..33982)
                     /locus_tag="VW325_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
     gene            complement(33984..35000)
                     /locus_tag="VW325_000519"
     CDS             complement(33984..35000)
                     /locus_tag="VW325_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9733976.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(34948..35889)
                     /locus_tag="VW325_000520"
     CDS             complement(34948..35889)
                     /locus_tag="VW325_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9733977.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(35965..36330)
                     /locus_tag="VW325_000521"
     CDS             complement(35965..36330)
                     /locus_tag="VW325_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9733978.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(36481..37539)
                     /locus_tag="VW325_000522"
     CDS             complement(36481..37539)
                     /locus_tag="VW325_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9733979.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(37702..38853)
                     /gene="nagA"
                     /locus_tag="VW325_000523"
     CDS             complement(37702..38853)
                     /gene="nagA"
                     /locus_tag="VW325_000523"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9733980.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(39006..40823)
                     /locus_tag="VW325_000524"
     CDS             complement(39006..40823)
                     /locus_tag="VW325_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9733981.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(40921..42063)
                     /gene="tgt"
                     /locus_tag="VW325_000525"
     CDS             complement(40921..42063)
                     /gene="tgt"
                     /locus_tag="VW325_000525"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9733982.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            42193..43050
                     /locus_tag="VW325_000526"
     CDS             42193..43050
                     /locus_tag="VW325_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9733983.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(43078..43722)
                     /gene="pcp"
                     /locus_tag="VW325_000527"
     CDS             complement(43078..43722)
                     /gene="pcp"
                     /locus_tag="VW325_000527"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9733984.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(43829..44188)
                     /locus_tag="VW325_000528"
     CDS             complement(43829..44188)
                     /locus_tag="VW325_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU9733985.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            complement(44199..44624)
                     /locus_tag="VW325_000529"
     CDS             complement(44199..44624)
                     /locus_tag="VW325_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9733986.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            complement(44947..46059)
                     /locus_tag="VW325_000530"
     CDS             complement(44947..46059)
                     /locus_tag="VW325_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9733987.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(46225..46845)
                     /locus_tag="VW325_000531"
     CDS             complement(46225..46845)
                     /locus_tag="VW325_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9733988.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(46849..48129)
                     /locus_tag="VW325_000532"
     CDS             complement(46849..48129)
                     /locus_tag="VW325_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9733989.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(48190..49674)
                     /locus_tag="VW325_000533"
     CDS             complement(48190..49674)
                     /locus_tag="VW325_000533"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9733990.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(49750..51027)
                     /locus_tag="VW325_000534"
     CDS             complement(49750..51027)
                     /locus_tag="VW325_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU9733991.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(51134..51217)
                     /locus_tag="VW325_000535"
     tRNA            complement(51134..51217)
                     /locus_tag="VW325_000535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51181..51183),aa:Leu,seq:caa)
     gene            complement(51227..51298)
                     /locus_tag="VW325_000536"
     tRNA            complement(51227..51298)
                     /locus_tag="VW325_000536"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51264..51266),aa:Gln,seq:ttg)
     gene            complement(51312..51384)
                     /locus_tag="VW325_000537"
     tRNA            complement(51312..51384)
                     /locus_tag="VW325_000537"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51349..51351),aa:His,seq:gtg)
     gene            complement(51399..51469)
                     /locus_tag="VW325_000538"
     tRNA            complement(51399..51469)
                     /locus_tag="VW325_000538"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51435..51437),aa:Trp,seq:cca)
     gene            complement(51474..51554)
                     /locus_tag="VW325_000539"
     tRNA            complement(51474..51554)
                     /locus_tag="VW325_000539"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51518..51520),aa:Tyr,seq:gta)
     gene            complement(51567..51639)
                     /locus_tag="VW325_000540"
     tRNA            complement(51567..51639)
                     /locus_tag="VW325_000540"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51604..51606),aa:Phe,seq:gaa)
     gene            complement(51643..51716)
                     /locus_tag="VW325_000541"
     tRNA            complement(51643..51716)
                     /locus_tag="VW325_000541"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51680..51682),aa:Met,seq:cat)
     gene            complement(51727..51816)
                     /locus_tag="VW325_000542"
     tRNA            complement(51727..51816)
                     /locus_tag="VW325_000542"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51778..51780),aa:Ser,seq:tga)
     gene            complement(51899..51972)
                     /locus_tag="VW325_000543"
     tRNA            complement(51899..51972)
                     /locus_tag="VW325_000543"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51936..51938),aa:Ile,seq:gat)
     gene            complement(52007..52077)
                     /locus_tag="VW325_000544"
     tRNA            complement(52007..52077)
                     /locus_tag="VW325_000544"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52043..52045),aa:Gly,seq:tcc)
BASE COUNT        14694 a        11987 c         9193 g        16207 t
ORIGIN      
        1 agccgtatat gtactcgttg tccaaattcc tgtttttgaa atatcctgct tgaatagtga
       61 gttcaagaat agatttcgac cggatgcctg cacactcgct atcttactag agagctcctc
      121 agctgtctgc gtgagttctg acttgctggc tttatccttg gttgcattca ggatttcctg
      181 acggatagag ccagctcgca cctcaaattc agcctgactc aacttctgat ttagtttgtt
      241 ctgcgtgtct gtctcaaggc tcttcacaga ttgccggata ttttcagcag tcacattgag
      301 tgagctgata tcggctttag ttctaagccc ttcagtcaga cggcttacac cagcgtcgag
      361 tgaatcagca cgctgcttaa agttggattc gactactgag acacggtctt cttggtcttc
      421 atacgctggt tgataggctg gaaaataatt accaaccgat aacatagcgt tctcaatgac
      481 gacctgcaga ccagcaggaa atccataatt agtaccaaaa cgaatgaaca cattattagt
      541 ctgatagtcc tcagaagaac tagacaagtc aatcgtaaac tcaaaatgtt ggcgttcgac
      601 agttccacct ttaaaaatta agtttctgta ggcataccat ggatgagcac taaaatgcac
      661 catagcaggc atgtcatttg ctagggcgac agggaaagtc acatcaaaag atatgcgaac
      721 ataatcacgc ttgaacctgt cactgttctt ccagaaatca ggaactatga atgttcgata
      781 gtcgtatacc gcttgacctc ctgttgtgaa cgttcttgaa cgtgaattcc tgaagtaatt
      841 ccgtgaacta cctgcctgca cactcgcaat ccgactagcc agctcctcag ctgtttgcgt
      901 gagttctgac ttgctggctt taccattggc caagttggtc agttctgaca gtctacgagt
      961 cgtcgtctcc tcatacgtcg cttgcgctga cttcacacca gccagttcat tcttagtccg
     1021 actaagtact tcaacttgct tggcaatctc agcttcagcc tgtgcttgct tcggtcgaat
     1081 atcgtttgcg atagttcgtt ttagaacatc caagtcacct gacaaagccg tctgagcgct
     1141 cgtagtctgc gacttaaacg cttcaagtct agcaacagaa tccagctcaa tccgcttcgc
     1201 ttcctgtgca agcagggtac ttgcgccagc cagttctttt ttggtctgaa caagtgcttc
     1261 aacttgcttg gcaatctcag cttcaacctg tgcttgcttc ggtcgaatat cattcacgat
     1321 agtccgtttc agagcgtcca agtcacctga cagagccgtt tgtgcgctcg tagtctgcga
     1381 cttaaacgct tcaagtctag cgacagaatc cagcccaatc cgcttcgctt cctgagcgag
     1441 taagctgctt gcgccagcat ttcgcaaagc ttcctcagcc ttgcgcttag tttctttcaa
     1501 tggcccgttg tcaaagctat taaatcgctg attgatagtg tcagacagtt ctctcttgac
     1561 ttcttcagct ctggctttgg ccagttctac ttgatcgtta aagtcttttt tgattttgtc
     1621 gaccttttgg tcaaaatctt tatctgttgc ttcaatctgc gcttggattt tcgcttcaat
     1681 gccaccttgt tgcttaatat gcttggtaat cgtaccctcg taagaatact gagtatcgtt
     1741 tccagcctta ctatctgcgc tgatacgccc tctcagacca cctttaaaag taaagctctg
     1801 acttaacacg ggaactttaa aagtctcttt cttgttggtc tgaatggtta cccactgccc
     1861 aacctcaagt aacaaatgtc cttggtagtt gagattatac ggatagtaag ttaggttttt
     1921 cagtttgtaa tacaggtcat ttaaagcgct ctgggtcatg aagacattgt ccagttccaa
     1981 agaccgacct gtcttcatac cgaccgtcag agacttcttg tccgtcttac aagtgatacc
     2041 agctatctga tactcaatct cactcttggt caagccatgc aagaagtaac tgtcagcgtt
     2101 gatcgtgata ttggactcag tcaaatcacg gatttccatc ttgccttctc tgttgaagaa
     2161 acaagacatc ccaatcatct gagtcatagc gctcagcata tccctaaagg aaagtttctt
     2221 gccctcagga acttgctcaa tatgataacg catcgcgctg attccgaaat agtcattcgc
     2281 taactcaatg cctgttttca ggcagatttc ctgaataacc tctcgtactt cagctgggaa
     2341 atgcaaatcc gtcacgtact cacgattgag cttaaacata ccgtccataa gttcaagtgt
     2401 ggttgtgttg cggtttcggt caatctcaat atcgttgatg aagtattccc ccatcttgac
     2461 ccactggtag gtatccccaa ccagtagacc aatctcaggg tgcagggtat ccagcttatt
     2521 gaacgtggta atgatactgg taaaggtaat tttaccgcta ccagcgcagg ttccaccagg
     2581 cttataagta tcgcccttga tgtagccata ctcaaaacta gcctctttga tatcccgtga
     2641 agcataatct ccaacacgga tagccagcgt cctgtttttg gcaaacatgg ctctgtcaaa
     2701 ttgtcgtcta gttaaagcgt ccattttctt acctctctac cagattaaat ttagcgccag
     2761 accaaggttt aaacttctca gtaaaggtat agctaggagc tgtcctatca ccgacataga
     2821 aagtctttgt gacttggcca tccatggggt ctggataaga cacctcaaaa aattcagatg
     2881 atacagcatg taaaagctga cttatttctc cctgagtcat cataccccat tcacagtcta
     2941 gtttgcgttt ggtcgtgata cggtcacgca ccatgtcgcc attggcatta cgccctgtct
     3001 ctccatcgat atcttgaata ccgactttaa aagatttggg aggcttcaca gccaccccat
     3061 tgattgtcaa ttgtgccatt taacctccta aatcttgagc aaggtttgac ctgctcgttc
     3121 atgttccttg ttgatttctt ggatagctac ccgtccgaac tcatggcctg cgatttgaat
     3181 aacgatgtcg ccgtcaccag aaaaaccacc ttgtggacta ataccagcca tggcatttac
     3241 taccgcactg ctgacaactc gcccaagggt ttggataaag cctgtatttt caagtggtac
     3301 gaccgcttct ttaccagctt caccaatcat ggcgattgtt gggctatcga cgataccacc
     3361 acgggcaaga cgaggtaggc tcacatatcc aataccacca agggatacgc caggtatttt
     3421 gttaatcatg ccaataacac cattgatcat gccgatgaag ccattgacta cattttcaat
     3481 agtcccaaga acaccattga ctgcacttct gaaagcccca cctacagcgc taccaacctt
     3541 ttgtccagca ttgacaaata tgctttgaac cgttgaccat acaccgctga agaagccacc
     3601 aattgaacta aacgcgttct tgactgcatt ataagcacta gtaaacatat tcccaaacca
     3661 agaagataca ttcgcaagaa cacttgtaac atctgcccat ctctcgccaa accataaacc
     3721 tagtttgcta aagatgtttg ttagaccagt ccatgctttt tggaacatgt cagtaaacca
     3781 tgccccgata ttagccaagg cactagtcac atccgcccaa cgttgtccaa accatgagcc
     3841 gattggtgtg aagatattaa cgatagcatc ccatgcttgc tggaacttct cgccaaacca
     3901 ttgacctatt ggctcaaaga tttcttgtag ttttgtccat agaccactga aaaattcgcc
     3961 aatagctcga caaataccac taataaaatc acatagtcct tgccatgcag ttttagcaaa
     4021 ctcaacaaca gtgtcccagt tttggtagag caagacacca atagcaatta aggctgcaat
     4081 agcagcaata actaaggtta ttggacttgt caaaactgct aacgctgcat taaaagccca
     4141 tgttgcagct gtagcgactc ctgctgccac agaatgtgca aattccgccg cggttgctaa
     4201 tcccattttc gctgtatgag cagtccatgc tagagctgat ttaccaagtt ctaaagcagt
     4261 tttccctagc tgtgcaattg ttttacctga attgacaaca aaatcttttg catacaacgc
     4321 attcaaatag atggtttctc cgaagctgac caatttatca aatgtcaaag ctttcaaagc
     4381 tagtccaaga tttttaattc cgctaacaat aaaggaaacc ttactactta ataattcgaa
     4441 tgcccctgca agtcctccag cttgttcagc ccaagacaag aacttaattc cttgccacac
     4501 ggttgcaagc gtaccaatca cactagcgat tgtggagata atctctttat tttctttaca
     4561 ccaatctgaa aaagcagtaa aaccatcggc tactagcttg attgtatcag ctagtaactt
     4621 caatgcctct agtatgatac cgcctagtaa atcagcgacg gtttcaatac ttatgccgaa
     4681 tgtgttagac aagaactctg caaaaggctt ccaacttcct tcccaaagta tttgaataat
     4741 gtcaattagc ccattaaaag cattagcaat agagttaata gcaggggcta catgttcatc
     4801 ataaacacga cttaagccat cgccaaattt gttaacagac ctttcaatgc tctcaaatac
     4861 aggcgcaaca gtatctaata aactttggaa gattgatgaa attttaggag cgtttgtcac
     4921 aacgactttt tcaaaatctt taaacaaact tcctgctaat ttactaccaa cttcaacaat
     4981 ggtagatgtc aaactcaaca gagttgacac aatagcgcta ccgatacgaa ccgcaccagt
     5041 tgaggtaatg acgtcgtaga aagcactaga aaagtcctga gcgatgtttc ctactgcctc
     5101 ggcaatgtta ccaatattat caaacaaagc gactagcgcc ttgataatgc gttctttttg
     5161 ccttccaagg ccattggcaa tactttcggc aaggaaaaca ccgataccta gcccgatagt
     5221 agctattgag cctgtcactt gccctaaagc ataagcaatt ttctcagcca ttcggttaaa
     5281 ggcattcaca acccttgggt cagtggcgat ttctcccatt gtcttagcta tttggtctaa
     5341 ggcagtctta atgcgtttta taccttctgg tctaaatgct gcatcaaaac ctttcttgaa
     5401 gaggtcaaac aaccctttga gcttatctcc aagaccatca aaaatgctct tgaatttgtt
     5461 gtccatgtcg gtcaactcga cttctggcaa gatgtctttg aaaggtccgc caccgcctcc
     5521 ctttccttta ccacctttgc caccgcctcc agacccgcct gcgtcgtcat cttttggttt
     5581 ttgcaagatg ttaatctcat caaatcccaa aagacctagc aactctttag cggccttctt
     5641 agcgtttttg gcggagtctc caagattgtc agcaagtcct cctgctgaat ctccagcgtc
     5701 gtctactgcg tcagcaaggt ctcctgctcc gcctgcagca tccttcatgg cgttacccat
     5761 gtctccaact gctccaccaa caccatcttt cactgttgct ttcttgttga acatcaaagc
     5821 gataaactca gcgagtttag cagtaacgtt ctttaagacc atagcaaaag agttcaagac
     5881 aggcataatg gcattgataa tcggtaacat agagttacca aggttcaatg ctgcgtcctt
     5941 cattagcgac ttaaacagac tgatacgacc atttacagaa ttagacaagg tatctccata
     6001 cttggctgta gcttgctcca ggatagccat aagacggatt tgttgctggg tttgaaagtc
     6061 gagttggtcc caactttgac catttgagaa acgtttaaaa gcttcagtgg acttaatcat
     6121 agccacattg acgttgattc ctaggtcctc aattgcttcg gtgttcccta gtagacctga
     6181 gcgaatccgc tccataacgt ctgtaatcgt gcgccctgaa ccttcagcaa ccactgccga
     6241 tgtctgcaac atcttagcag tataggcgct tagcttattg gtatctttga taaatccaga
     6301 aaacaggttg gagtaaaccg caccgtacct ggccgcttca cctacaccca tattcattgc
     6361 atttgcgtta tcgttaaccc attttaagaa agattgcgaa ctctcgccca tctgacgctt
     6421 gatttggttc atagacgctg acacttcaag agctgtctgc gttgaataca tcccaacatc
     6481 aagtaatttc ttaccaagga ttgcaaaacc agcgaactta gccagcttgc caaacgcact
     6541 accgatagag ttcgactgtt cacgaacttt ggcagtggca tttttcactt ggtcagatgt
     6601 tcctttgacc tgattctcga cttctttcat cttcttcctg aaaggcgcta tctcagcgtc
     6661 aatcatgacc ttcaattcat caagagttgc cattcacttc ctcctttctt ttgcgattgt
     6721 gtctctctgc aaaatcacgc atccgttcct tatgcaacaa aagcgcttgt ctctgtcgtt
     6781 cctgttctac tgcttgctgt tcttctacaa acaactcagg cgcatattcc cagaactcaa
     6841 aaaccttggc atccttggac aataacaaag aaacgtggtt ggatatcatc tgcgaaagtc
     6901 tgtatgagtc aataatcttc tctttacgct cttggatttt gacacggttg tagctttcta
     6961 tcatttccct gatttcaagc accgtcaaat cccaaaaatc aagaggctta cctccgatgt
     7021 ccaaaaacat tgggtaaagt ctctcaataa tctgcgttac cgttaagatt actggactac
     7081 tgtcattttc ttcttggaag ttttcttgtc cttgcttcct cgtggagtaa aacccgatac
     7141 ttcaaagagt ggcattaaca cctctgtcat gaatgttgtt tggtctccac cattgtccac
     7201 gtattcatcg tataaatcat agacatcctc aagagaatac ccatgttcat actgctgcaa
     7261 ggcgccgtga actaacaaca acataacttt caaaggtggc aaagtgaact cttcgcctgc
     7321 ttcaggcatg aaaatcttca gcaagttcat gccgattttt tcttccacag ttgcagcttg
     7381 atgagatgtc aaacgtagct tcaactcttt ttcgtcagta actttccaag ttgtgtattt
     7441 taacgccatt taattaacct ccaacaccat ctgcaaattc caactctgac tgtaaagcaa
     7501 ttttaagggt gaaatcgata acggcattga caccgccacc gccaagtttt acggatactt
     7561 gaccttcaaa tgtgacctta gtaccgtctg gataagcttg ttcgaagtag agtttttcct
     7621 tgtcgtctgc tgccttacgc aatacacggt aaggagcagt tgcgcttgaa ttattataag
     7681 agaacttgta ctcaagttct cctacgtccc caataccaag ctcgtacttc ttcaccgtat
     7741 cttcaagagt agtattttct actttttcga gttcaatacc aaactctggc acttctttca
     7801 atccaacaag cttagtatag ctaccttttg ttttgctata agaaagcgta attccatttg
     7861 ctaacatata ttttaattct ccattctaaa ttgaaaaaca agctctgaat ctaagtcaac
     7921 gacaccttca aaacgcatga ccttatgtct caaatgagac gggtctggca cgtcttggca
     7981 gtcggttctt cgcaaaccta aagactcaaa aatctgattg attttaacag ctaactcact
     8041 agtgctggta tcatcaaaaa tatccacctt atagcggata gaggattttt gttccttgtc
     8101 gtcaaaccaa tcacctggct tgttttgttc ttctaaaaaa ataacgactg ggaaagtctc
     8161 ccaatcgcta ggataagtat cggtcacatt atctgcaacc ttttgcaatt ctttataaat
     8221 aacaggcttg atattgatca ttgtatttgt tctcttatct ttctacggac ataattcgaa
     8281 atattcttag acacacgctc ttgattgtct ctcaaagctg gataaagata aggctgagca
     8341 ggttgaccat acatcttgta aaactcccca atcttttgaa agtggtaaga tcctacattg
     8401 atttggtctt catgcacata ccaaggatga gacttgtaag tcacgctgac ctctggagag
     8461 ataccagaat ggctagcctg acccttaggt cccgttccaa actcaacgta aggagcgtat
     8521 ttaaggttgg tgtaaacttc gcctatagcc ctatctccgt ccatttttgc tctggttttg
     8581 atactagtta taagttctcc atcattcact ggtacgagtc ttcttgcatc agcttggaca
     8641 acttttatag cagcattgtg taccgcacgt aagacgatat cctcgccagt ttttttacta
     8701 gctaaccgtc tacatttagc tataagccta tctgccccta gtagccctga cacgctctaa
     8761 ctccaaaact tgatgatgtg tgtagacctt tttagaaata accctatgag tcacttccgt
     8821 ctgactatcg atacacacac catcttttac tttgatagta gctgacttgt tggcatttgc
     8881 gttcaaaata tcattgacac gctcgccata caattcagat tgtagcttgc tactagctgg
     8941 ccacaattca aggcggactg tctcagcttc cttggcatac ccttctttcg cgacaccttc
     9001 ctcagtgaca gtcttttcaa accgtcgcat tggatagggt ttcagtctac tctgcttcaa
     9061 aaacatgacc tgccaccctt gctagcctgt gcatgcgtat tcgctgtaga agacccgtag
     9121 ataggccgtt ttctccgtag actactgcta taccaccctc ggttctagaa tgctctcctt
     9181 ccgctcctga gcggttgtgg agctcgatag caacctcagg tatcaaaaga cttaaagcag
     9241 gtgtcaaaga tgtgcgatta gtctctgaca agataagatt tgtagccctc gtttggagca
     9301 acatgagaag ctgagtatct tcttcgcctg ttaatttctt cagcaactct atagacatat
     9361 caatcctctt ctaagaactc aggttcaggg aggattttct caagaacgtc tgagatagtg
     9421 acaccgttgc tggcaatatt gtcagccagc tcagcatagc gctcctcagt aatctcaagt
     9481 tcctctcctg ccagtcgttt cacatttgat tcccaatcat agaaatcttg tttgatttta
     9541 aatttcactt tttagttcct ccaaaacttc tacaatttcg gcttttgtca acttataagc
     9601 gcctgctaca cctgcttctt tcgctagatc cttcaactct tctagagttt tattctctaa
     9661 atcagaatac tggttaacct gctcttcttg aatataatga cgtcgtagca ataaactcat
     9721 atcgtcacct cttacgcgcc accgaatttt acaactcgtg tagggtcgta aaggtaaacg
     9781 ccgtagtgtt catcacctgt gattacggta gtcttcttca agatatcgcg gtctgtttcg
     9841 atagccacat cacgtttaag caagataaca aaagctccat atttgttagc gtcgtctgtc
     9901 tgagtttgac ttggtgacac tttaacaagg aagcctttac cttcatcaac ttttttagag
     9961 cgcacgattt gcacaccatc aacttcaccg aaggttccag aaacaaccat atttgctcca
    10021 agctctgaac ctttaatcca ttcttttgct actgcagttt ttagcttagt agcatcttta
    10081 gggttgatga tagcaacata ctgtgcatct tcttcgtcct caaagatatc tagagcttta
    10141 tcgattgcct caagagttgt tggagtttct gtaatatgtt gtgttgcagt tttagctacc
    10201 gctaccaaat cattatcgat cttgttggca atagctaaac caagctggta agtcgcttga
    10261 cctagtgggt caccaagacc tgacaaaaga gactcatcgg taatttcata acctttacct
    10321 gcttttttga tggtcatagt agtcttttta gtagtcaatt ggtctggcgt aattgcttgg
    10381 ccttcttcaa tttcagtagc gtctcctgca tactcccatg ctggcactgt taaagtgcta
    10441 cctggttgac cttcaagctt tgtctctaca tacgctagcg gtgtaaattt aatcaattta
    10501 ggtaatttag cggctaccat atccgccatt acttctggat taaccatagt ggctaattta
    10561 gtttgtcctg ctgtcatttt ctattatcct ttcaatttct tataaagctc tgggttcttt
    10621 tgatagagct catttcgact ctgataaccc atacgagcaa attcttcttt tgtgataccg
    10681 tcactatcga ctggcgcttg tttcattgga gctccgcctt ttagtttttc ttgaacgcct
    10741 ttttgcacgg cttgctccca tgatttctgc aatacagcga cagactgcga taccgtctct
    10801 gcgcttgtca aatcaactac atttactaac tcaacaggta agtcacgttc acttagcatt
    10861 gctttagctt ctgcggtcaa ttccttgcga gcaatagcct tctcacggtc agctagttct
    10921 tgctcacgct gatccaactg atatttttgt ttctcgtcag cgttcatctt ggcaagtttc
    10981 ttagcttcgt tttccttggc ttcttgctca gcttcccatt tagagcgttc ggcagatagc
    11041 atcttaccga tttcagcacg agtgaaagtt cgttcgtgct tttcttcctg cactgaatca
    11101 acatttcctt gagtgtcgac agtctcagtt gattcagtag atacagttgc attgatttct
    11161 tctgacataa ttgtcctcca gcgattacgt cgccactcga taatctcgtt ttacgtccgg
    11221 cgacggaaca gtacagcttt taatgtcatc ggtacagttt ggacaatata aaaaccgtac
    11281 ggggttccat acggttaggg cataagaaaa ccgcctcgat ttcgatgcgg tttatagcaa
    11341 tttatagcag tctattcctg caagtcaaga tgttggatca cctcctaatc tttaatggca
    11401 cgatttgaaa ctttggcgta aacatccaca taagtctctt tcttatctcc gttatgcgtg
    11461 atttctgcat aatttccaca aggttcgctt gatgtaattg cgttcgtact aacaagagct
    11521 ttccagtttt gcagggtctt gctaaaccaa actacaaagc agtcttctgc tttgatttca
    11581 cgacctgata agcgtgaaaa ttcttgcgat gccaattgtt ttgctttttc taacatttta
    11641 ttcctccgtt ttttcatatg tttctgcaaa aatatccagc ttgcatggat aatattctcc
    11701 ttgcacgcct ttgataatat aatccccttt tttagctacc atatcacctt caagtgtagc
    11761 aatccatata ttcccaagtg catcaaacca aatcttattt tctgcaaagt caattacctc
    11821 tttatggtta ttgccgttcc actgcacagc ctcgaccact actggttttt tacggtattt
    11881 cattcctcac tcctttctgg gtacgaaaaa agcacttaga tctctctagg tgcttaagta
    11941 gtgaattgca tttttatatt ttttaacacg ctcgtagtct gtattggtaa cagatttcaa
    12001 acgtgataaa tctgagttat gtttcaaatc tgcaagtttt acaactcttg ctaaattatt
    12061 tgattttact ttcccaagat attcttgata actttgacct tttttctttg tcaaaatttg
    12121 taccgctgta acaacttcat ttgacaagcc agacgcgaat aaatcggcag cagttatatc
    12181 gctatcctca atcacatcat gtaaaagagc gacagctttt tcttgttcag tgttgacttg
    12241 actggccaca tagagaggat gctgtatgta atcaacaccc gctttatcta cctgccctgc
    12301 atgtgctttt ttagcgatag ccaaggcaat atcaatcatg ccgctaccat cctgtcaata
    12361 taagtaaatg catcattttc tgaaatttct tcaaaatccg taaagtcatt aaaaaagatt
    12421 ttattaaacc aatccatgct attaacccac tttttttcaa tgtcaaaaac ttgcatgaca
    12481 ccatcaatca aacgaagtac ttgagcattg ttcgtcgttg tgcggtagta tttaatatct
    12541 ttcatatcac ttcaccctct ctatattttt aggaatctca agcccattgc ttaaatcaag
    12601 catttcctta aataatttca tgcgttctag atcagatgta ttcgtatcac gatacttctc
    12661 atagagttca tgtaatgaat cattttttaa gtcgaaactt tcctgagtat gatactgcat
    12721 ttcaaagttg ataccatctt tttcaacgac tgtattcaca cctttgtatg gtccatctac
    12781 tagccaagtg ttttttactt ttacaatttt ataaccttct gcgataagct tctgtttcat
    12841 cttcaaatac tcttttgcga aagtatcggg atcgaaaata gttgtatacc gcaaagcatc
    12901 gttaatttta cttgtagcct ttgataaact tatattttca gcttggctat ctgttgtaat
    12961 tttacgtgat aatgactcaa ctgttttctt cctaaattca agacctgcca atttgttttc
    13021 tcctgcgata cgttgcatat cacttgtaat ttttggctca actcctgaaa ttttggacaa
    13081 tagttgttcg ctataaaatt tcgccttggc ttctcttgta tcttgattat acaccttttc
    13141 cccgtctttc gcaacatact tgctatacca ctctttataa gtcatatcgg caggcacgta
    13201 ctcaacttta cctgtctctg gattccttgc tctgcgcttc aacttgctgt agtctgcgtc
    13261 ctcatcgtat ccgacagtag tagacctaca ccaagggtgc ataggcggac aattgacacc
    13321 agggacagcc ttatccctat catagacctg attgtcatgc tcctgacaaa tgcgtgatgt
    13381 acgcttgtct aagacggcca caaagatata cttttctatg tctgcttctt catagctgag
    13441 tagctccatc tggttatgaa aaaaggcgga ttctgttcga accaaacgcc ttgcatcgtt
    13501 ctgacctaca ttgaacctct cagcaattgc ttgtgcagtt tctcgtgtat ctctgcctgt
    13561 catgaggcta atgaggagtt catcttttat gctggaagta agttttcctg tattcttcca
    13621 gatgtctgtt gagtacgtac ttccatctcc tacccaacta aaagactgta gatgtttaat
    13681 ctcgttctca ggaagcccag aaaagccata tgctagtcct gtctgctgtt gtaggtcaaa
    13741 ggtagccttg tagtaactat ccttcatcag gtcgctataa aaggcatctg agcctgtctt
    13801 ctccgaatga tagatagatt cacgcatacg atctaaatcg tcgctcaaac gctctaggcg
    13861 cttcatgcgg aaagaataag ctgggctgtc taagtcagct agtaatcttt ggatgttcgg
    13921 gtcattcggt ctcgcttcaa gtactttacg aagttcattc agatttttct tgtctttcat
    13981 gttcttcaag acttgtctag cttctacctg actcagacca taatcacgtt ggaatttatc
    14041 gaaaatctta ttgatttcct tatccaagta agtcttagct tcctgataga ccttatcgaa
    14101 ctggtctgcc tgcttttcgg ccttgtccat ctgctggtaa atcagattgg ctttcctctt
    14161 cgcccaatac tcctgattct tcatcctcta cctcgtcttc gggtttcgtg ttgtctttgt
    14221 tgaacatcgg catgtcttcc atgttcttct ttttctcttc ttccaaggct tccagctcag
    14281 cgtcagggtc ttccacaaac ggcaagagag aaataagctg tctattggtc actttgcctt
    14341 ccaaattgtt cacaatctga gagatttcta ataagttctt aggcaaacca cgactgaatt
    14401 gtggaacgat tgaatgagac tctaaagcaa tctgcttcat gcctaagtaa tgagcaaaaa
    14461 tcgcaatacg ctgacgcaat cctcgcttat agttcgcttc cttggtctta gtaatcatct
    14521 caaggcccat cagcttaaat tccatggcta cgcctgatgt attccctgcg aaattctcat
    14581 cagtcaaatt aggcacatgg ctaaatgtgt agatgtcctc tttaagagct gtacgcaaga
    14641 tttcagtagc actttcgtcc agcgtattct tcaagaactc agcccttgca ctatcgcccg
    14701 gcaattccaa aagaccttct tcagaaagaa tcttcattgc taccttagcg tcttctggag
    14761 tgtctgctaa ctgcgtgcca tacaagacaa gtatagactc tacagcctgt tccttatcat
    14821 tgacacgatt ccccatcaag gaattataag cgtctatcaa gctaatttgt tgctcgtagt
    14881 cgccaatcgc aaagtgatta ttgcgatact cgataattgg gatttgacca aggttgtgag
    14941 gtgttgcctc ctcgctctga gttgttcctg aatctgtact tctcagcacc atgtgatagt
    15001 gcagattttc ggtaaagacc tcagcctggt gcttggtagt gtctttcgta tcgtctttta
    15061 cttcatagta atagaccgca aacaaaggct tccgctcaat actatcatcg tagaccatga
    15121 aagtattctc cggatcaata ctagttgaat ccaactcagt cataccctct ttagcataga
    15181 tgtactcgta agcacggccg tagatagcca tatttagtgc gttctgcgca tccacttggt
    15241 caatctcagc accatcaaag gctgtaagta gttcatcaat atcaccgtca gcagtattgt
    15301 tatacttgat aggattgccc ataaaatagc ccgtagccgt gtctgcgata tccttggcat
    15361 gattagctac cgtcttgtaa ttgggtgcgt tcacgttgcg tctcgtgtgt tttaagatag
    15421 catgctcgcc catgtagtag cttttaagct tcttcaaatg tgaactttca gtgttatgta
    15481 tcgttatcaa tttgtaaatc aggtctttct tcaaagaacc ctcatcatat ccatcccgtg
    15541 gataggttaa atattggtac atgtctttcc tctctataga ccataatcag aacgtctgcg
    15601 gacggttgct ttcccacctt cgatacattg aaggctgtaa cgcaaagcgt ccatcaagtg
    15661 gttgttttta tcctctggtt tattcaacca gttgccttct ttatctcgct ggtagcagta
    15721 actataaaat tcatccatga tgtttttaca atctggatgc acataaatag cgtatccttg
    15781 caatttggat acacctgcca taatactatc cttacctttc cgactctctt ttattctaga
    15841 tatgccatgt tctgacctga gctcttcaat cagtcgtaat tcagcgctat cagcaatgat
    15901 ttgtgagcga tgataacctt tgtcctttat catcttcgca acttctttgg ttatcaatcc
    15961 gactttatac gcctcatcaa agacataaat ctctttcgtc gtgtcattta tcaacgaaca
    16021 acacaaagca gttggatcat gagtaaaacc aaagtcaaga ccgatacata acttattagc
    16081 tgaatcttgt agtaattcat ctttattgaa atccttgaca gtcacgtttt cgtagattaa
    16141 accttcagca actccccatt cgccatcaca aacgattcta gcacgtctgg ggttcgtatg
    16201 atacaaatcc tcatagcgtt tgatatcgac ttcatcaagc cactcattgc atttgtaagt
    16261 ggttgtagta gcgaatgtgt cagcccgtct cgtctcttca tcgaagaata cacgcttgag
    16321 ccaatgcctc tcattccacg ggttaaatgt gactgtgatt tgtttaaaga aatcaggtac
    16381 atctaagcta ccacggatag actcaacaac cgtgctgaac ttgtcttcag tctcaatttg
    16441 atatgcttcc tcgaaccatg cccaacaaag actgccgaca tcgaccgtga tagatgtgat
    16501 tttgagttca tcatccaaac cacggaatag gattttttga ccagtcgctt ttatggttat
    16561 ttcaggcaaa gactcattga atttaaacaa atgagtcacc cccaacacat tacacgccca
    16621 tttaaaatcc gtataagttg attgcttatt tgtattcgaa tacctacgaa taacaagcaa
    16681 gttggcccag gaatatttca aaagacggac aacatagttt aaagcggttg tcttggactt
    16741 cttcgaacca cgggaccctt tgactacacg ataaagactt cttgagcgcc agaactgtcc
    16801 gtacccagct cctactgtct taggtaagtc aacgacaata tcgttttgct taatctggta
    16861 tgtctgactc atttgcaaac accaccgttc cagaaacatc cgcctctacc ttatccgtcc
    16921 aaagcctatg ccgtttacca agaagttcag ccgccttgat tctatcttta gctcctacat
    16981 ctatatccgt aatcgtctgc cctagttctc caatgcttat caaggtctgt tcttgcgtct
    17041 ctcctcgcat gactgaagtt agataaccta agacctcttg ctgatccgca atcttttcag
    17101 aatcaagctg tttcagtcgt tcatctatat agcttttaat cttaggattc tttagtaact
    17161 tatgtccttc aacgcctgcc actctatcac tagaaacacg ataacctgct ttcttatagg
    17221 cttccgtcgc attacctgag atgatgtact catctgcaaa tctcttttgt tttattctca
    17281 atccactcaa ttttccatca ccacctttca aacaatcaaa aaagccacac gatgtgcgac
    17341 cttcttgcaa ggcgactact accttgcgtg cgtattaaat tttgacttct tttttatttt
    17401 ttgtagtctt taaaacctct gagggaatca aaccctctag cttataactt atccggaata
    17461 taattagcta cgcaatcatg cgaggtccag tcgcttccgc aaccattttt aagttaatga
    17521 gtgataggag ttaatgagtg atatgtgaat ccccacccag aagatttaac tcattctggg
    17581 acacaaacac tcaaaggaga ggggaggact tgaacctcca aggccattac agccccctga
    17641 cattacaggt aaccatctac caattctgag acctctcttt tcaattcttg atactaccat
    17701 tctaacagat ttttagaacc gtgctgttcc aaaaagtccc ataagctcac tatgaggtta
    17761 gataacttct tccaaagcta agaccgcctc attttttaac ctgtagtagg ttgtacgact
    17821 catattcaaa tcataacaaa cgctatcagc ggtgcctttg ttgatgtaag tcattcttaa
    17881 tactgccctg tactttggat ttttaagcct attgatcatt ctacctaatt caagttttct
    17941 gttaatgacc tctttagtat cctgctctat agcctctttc atcactacca actgagtata
    18001 gacatcatca acttttctag tctgtccacc ttggacttta acctcggacc atttagggct
    18061 tgagagcaaa cctgcctcaa gctcgttaat ttcgtctata cggctttgga tgtccatgtc
    18121 cagatcctgc aactctttca agagctcttt agccttgttc actctctgtc tcctttgtga
    18181 tataatagtc tttgcgagaa ctattagctg aggcagagag tgtcttggct ttttttaatg
    18241 cttaaattcg ttgaccaggt cccggataaa gaacttccaa tcagattctc taaacgtcaa
    18301 gaaacgatct gtagtaaaat ttctaagtct tttatagaaa agcatcttta gttggattga
    18361 ctcaccaaca ctcagtaaaa taccggggaa gcgatgtact gaatgcactc tatttccgta
    18421 tccagaaata tctaaatgta ttaacgtttc tggatatatg cgccccatac tagcttcaac
    18481 tccgaactca accttaactt cttctacaat tggaacttcg tcaaaaattg gtcgtgcaga
    18541 aaatattggc gacggcgttt cttgtttttt tcttcttcct gaatatggat atttacaagg
    18601 tctcatttgc gtcctccaaa ctccttattt tcatagatgt tgccgatgat ttcattttct
    18661 tcaatttcag tccataaaca tactgcgtca ctgcccgtat caattaccca agaaccctca
    18721 agctgcttaa caatccctat aaattccttg tcatactcat agaaaccgcc aatttcgtca
    18781 gctctaccca aaaatctagt agttcgtaca atatcgcctt caaagatttc cttgccgttc
    18841 ttgtctttga gtcctgttga ttgcatgagt tcgatttcgt caaaatcata acaatagata
    18901 tctctatcgt ctggtaaacc attctcaaaa taaacttgtt gtgtcactat ttctttgttt
    18961 tcgtagtcaa tagcaagaat gtcatctgaa aaaaacatac gtttttctgt ttttatccac
    19021 gctctatatc ttggtgtcat gttaaatcct cctaagcatt aacaactgga aaatgaatat
    19081 caccaatcac taaagagcat acgctgtaat aatagccgtt atgctctgct tcacaattgg
    19141 caatagctac agggttctga ttatggaaga tagttacttt gtttttataa ccagttcccc
    19201 aatggtcggg gatttcttcc ggttctccaa tttcaatatt agtaatcaca gcgtcaagtg
    19261 atacatcttg gaactcccca cctgctgagg cacagcaatc actttcagac atttcaatag
    19321 tgacctttgt gccgtcttca agcagcaaaa agtccttatc ccatttcaca atacgcttaa
    19381 agagcaacaa ttctttaagc tcttccaacg acccgtacct tgcatttttc caatcaggct
    19441 cataatagtc tggtagttta atagtttctg tcatagtaac acctcatccc caactttcac
    19501 tttatcgtac acgtccttcg taaccacgaa caccccgtag tcacgaatgg taagcgtata
    19561 tagcttgcca tgccgtcctt tctcgacgac tttaccgaat atctcagcgc ctgcgttatc
    19621 agctttatag acgacaatcg ggcgcttttc ttctaacttt ttaatgtaga tactctgcca
    19681 gatattcaat ccagcagata gcagaatcca gatagctatg aatcgtttca atttatgacc
    19741 tcctccttca atttaagctc aatctctaag taaaagcttt gatcaggtat ctccagtatc
    19801 gctgtagtgg ttttaccatc agaaccaacg ataatttctc cgattgccaa aactaagtct
    19861 ccaattgtgc tatttagcgt aaggctcatc actccacctc ctcaatctca atccctggac
    19921 aatcaaacac ccagccgaag tttgcgtctt ctagttcttt ttgagtacct ttataattcc
    19981 tagctgttat atcttgacta aaataaagag tactccctga ttgcgatttg accaaaggct
    20041 gcctattttt taaagtcacc aaataccgct tctctttttc aacctcgtag ccgtcaagcc
    20101 aagcaagaca gaatttttcg atgttatttt cgtaaaacca ctcaggaact ttcttatcat
    20161 aatgatcttc aattactctc attgcaccgt aaacatgaaa attgtttttc tttttaaatt
    20221 ctatatattc cgccacacac tgcggaactt tgactggttt tggtttatct agttgttcca
    20281 agtcttgcag aaaaatttga cgagctagtt ctgctccttc agcattccat acaccctcaa
    20341 gttttttata tttctcaatc aattcctgct tattcatctt agtttcctcc ataaatcaaa
    20401 taaactgcaa taactacctg agccatgctt ggcgaatagc caatccaatc atcaaactcc
    20461 ttagattttg gcaaccaacc cttagtagct cccaaatcat agtctgtagg cttttcatca
    20521 gcaaagatgc attccatcgc tcccataaac gtcataccat cttctgccat ttcccaaaaa
    20581 tagtccgccc ggtctttcac cgcttgtggt aaatcttgct tgggaggttg cggcttcccg
    20641 tcttctaccg tccagttgta tacttcatta actttttgct ttaactcttc catcatcttc
    20701 caacttcttc actttccgtc ttaattctca ttatcttcct cctcaatttt aataacggcc
    20761 ctaccgtttg ggtgtcgtct ttggtgtgat gtgtaagtgt aatactttaa catcctttca
    20821 gtaattcctg tctcgctact gatctgcgct aatgttccaa gcgtaacaaa cacatcacct
    20881 tgatataatg cgtagtcagc catctgctcc tcatttcttc aaatactcag ggatttcatg
    20941 acctcaatct caacctctat ccgtggattt agactgtaga acttgcctac atcatgtagc
    21001 gctatctgac cgtcgtcctg gaagacgatc cctgacatgc tgtcatatag cgctttttcg
    21061 tagttatcta tgtcaggctt tttgcctact ggtataattt catccaggag ggcctgttgg
    21121 ttcttcttga ctttagaaat gtactgagga ggtttgatat aaaatctaag ccgtgccctc
    21181 aaagctccct caaggatagg ctgacccatg tactgattag caatgagcag ctggcaatga
    21241 ttgcgccatg ttttcatatc cttgtcttcg taagttgtgg taaaactccc acgtcttgca
    21301 aaccttggcc gtgattgagg tttaggctca atgttcaggg tcaatttcat tcaagagccc
    21361 ccttaaatcc tgccatctca aagagatttt ctctgttttc gtttacgaac tcaaagaatt
    21421 ttttaacctc ttgtagcgtc ttgatgttgc tcttgactcg tgttaatgag gtgaaaaata
    21481 catcattttt gggaattgcc ttaactttgc acttgtagac cggttcaaaa aggtcaccat
    21541 tgtcatctag tgtaggagcc gtgtctttgt tatcaaagct aatgctcata tcatagttta
    21601 gagtcgtaac gacctctatt ttttgtttat caatgatgat agcaatacgt tctgtcgcat
    21661 tgattttact tgccatgttc tttctcctgt taaaaaagtg tcgtttgcaa agggtacaca
    21721 tcttcaaacg gtactccaag tcttagacag tctcgtttga tgtccagtgt agagatgacg
    21781 tacttgacgc cgttgttttt cttgtcatag tgtggaaaag tgtacccgtc attttcaatt
    21841 ttggttttaa tgtccgtttt ggtttcaggt ttccaatcca cccaatccgt ccactccatc
    21901 tcatacctca tcaaataaac ttaattgagc gttgtggttt tttatccgct cctctgctat
    21961 accaaaataa tattcatcta tttcagttcc tataaaatgc ctatccgttt ctaacgctgc
    22021 taatgctgtc gtaccgcttc ccatgaaacc atcaaaaaca gtatccccaa catcagaatg
    22081 tttaacaata catcgtttta tcaattcaat aggtttttga ttttgatgga gtagtttatc
    22141 tgaacttact ctcttgaaat cccaaacatc tgtcagtcgc tcaccgttga atttcttccg
    22201 tcctttgttt gccaatataa tcatctcgta ctgttttcca aactgcgcct ctaaatcgcc
    22261 agcggtatga ttattttttc gccatataat aatatttttt actgaaaaat acttttcaat
    22321 ttctttttta aaaaaatcaa ccttgtcaaa tgaacagaat atataaatag ccgtatcatc
    22381 ttttaaaatt cgataacact cttttatata ctcttttatc aattcaggat tgttatcatt
    22441 tttgataaca tttgaaaatt tatgcttttc tttccgccaa tttgttttat aatttatcaa
    22501 ataaggcggg tctgttacga ttaaatcaat tttattatca ggaattgttt ccataaattc
    22561 caagacatct ttttttataa tcttatctat ttccactaac tgtccctcct aaaacggcaa
    22621 accgtcattt gggaggtcaa aggggttagg atcggtaaaa ggtgagctat tcccattttg
    22681 gaaactgttg ccttgtccgt gctgactgtt gcgactctct agcagagcta cactctcagc
    22741 gattacttca gtcacatatc gacgctgacc gtctttctcg taagacctaa cttgtaagcg
    22801 ccctgtgagg ccaataagtg agcccttgct gcaatactga gcaatgatgt cagctgtacc
    22861 tctccaagct tgaaaattga taaaatcagc ctcacgctct ccattttcgt ttttgaaatt
    22921 gcgattgact gcaagcgtgc cctgtaaact agatacatta ttaggcgttt ttcgtagatc
    22981 aggaggcgct acaagcctcc caaccagtgt gacgttattg atcatctgat ttgtccccct
    23041 ctagtgctac gctctcccaa gagataccct aaaaacatcc ataggatagc catcccaatc
    23101 tctttgataa aatcattcat tatttctctc ctttgcattc ataacataca ttttgaccta
    23161 catcttttcc cttgattatt gataagctac cacatttctc acagctgatt atgaaaccta
    23221 aaccatttga attaatactg cttatattgt tctctgaggg aactttgtaa ataatcaatg
    23281 cggatgtatg ccaatattca gcactgactc cactgtcagc gacagcagac acatttgatt
    23341 gaaatttgat gtcaatcaac ttaatgcctg gattttcggc aagccagcta tttatttggt
    23401 cgtcaatcgc ctcatgatgt ggataatcac atgaaaaaaa tacggtttta atcatattcc
    23461 cctcctggat tgtgccacca gatcatcagg tcttcctgat tatctctgat gtactgctca
    23521 aatatttcaa agtggacgat agcatgtttt aagcgttgca taccctctcc agattttgag
    23581 caaaagctga aaactttaaa gacaggctca atcatgtcaa taatttctac gacttggcca
    23641 ttgaggttcc agacgctatc ctctcccaca ttgaaatcaa ggataaactc atcccctagg
    23701 ttgtggataa cctgcaattt cttgccgtcc gagtagatgg ctacgctgtc agatattttt
    23761 ctgatgtcca tggttaccct ccccattgac tctggagaaa tatcccaaga tttttctatc
    23821 tccaatttcc ttttttcgcc atacagagtc gctaataggt cctctatttt tcctattaac
    23881 tcatcaggca ccccatattc agccaattct tctgaaattt tttcaatttc tgtcatactt
    23941 accacccaca ttgttcattt agctcagcct gagttaatgg atcgatacgt tgataaccgc
    24001 tgacttgata gttcttttta aaatcaaatc cgagtttact tagaccagcc ttgaaacagt
    24061 ctttttcggc tgtgtctaca aaatacacct ctaaagtcat tttttgggca tatcgtttta
    24121 ggtcattttc agcccctcta agagcgttag gctcattttg gaggatttgt ccaccgtcca
    24181 agattttgcc cgtttctggg tcaaaatttg gggtttccgt tgattttgga gcctgttctt
    24241 gctgtttggt ttgttgggct gctaaaagct cctgattaga tcgctctgct cgttctagat
    24301 cctgtctgag ttcttccttt tgcttttcaa actcataatc agttttgatt tgttcaaaga
    24361 cttcagcaag agtcaagtct ttcagctgtc taatgtaagg tgagtcagtc atgccgtact
    24421 cagcacataa ccctgaaata gctgacttgg ctttttcaaa ttcttgttgt ttctgaaatt
    24481 caaatgtgac catgtcatca agtgacttca tagtggcttt tttaagcgtc acgccatctg
    24541 ccataaaatc gctagctttg acatactcaa gggccttttc atcaaagaga cgaggatcca
    24601 gcatgtactc agctgatttg ttggctaggt agcctttgac tgtgtcaatt cggacagcct
    24661 tttgatgttc ttcaaactct ttgacatcac cagcaatttt ggtaatgatg tcttttagag
    24721 gctggatggc attcttgaca tacttgtcaa attcatcagc tggttcagat aagactttct
    24781 tattcctgat ccgttcatca gaaacctgct tgtctaattt tcgtagatcg gcaagtgtct
    24841 gcttgtcatc cttgatagtt gcagccgtaa ccgtgtaatt ttgatacttt gctacaacct
    24901 cattgatatt ctgctcaaat ttctcacggt caatgatttc aacctgtgct tgtgttactt
    24961 ttacctgtaa ttcttgcatg ttgtcctcct aatattcaag ttcaccgtct agcaactcgc
    25021 cctggattgg atcctcattt tgagtaggtt caggatctgc atgatttgcc tcttgctctt
    25081 tgttgaattg atcaatctga gccatcttgc gtgctacgac atcctcacgg ctctcttgag
    25141 gtgtgacgtc tttgattgcg tcaaatgtat ctccaccatc gatgtctgta tacatgttcc
    25201 caagttcgtc agggaaagcc tctctaagag tctgcactag agctgttttt ctgatcatag
    25261 tggctggcat tgatttccaa gtgctccgtg gattgccatt tctgtctgtt ttgacatact
    25321 cattaaagtc aactgtgacc ttatatctgt gtgaacggtc tttacgatag acaacggccc
    25381 aaccacctat tagaatgtcc tcaggtaatt taagagagcc ctcaatctct atcatttctc
    25441 catttctcat gacagtaatt ccagcctcaa aaccatcata tccctcacaa cgttcagcac
    25501 gtttcataaa tgcctctttt gagacaatca agctgaactc tgtgccgcta ttgttttgat
    25561 aggctacaat gtagacctcg ttagcaaatg ggttaagatt gcgaccttta cacaaggcta
    25621 gagcctgacc tacttgtttt tcagtcagta ggttttgtgg gtcaaaatac tttttgatgt
    25681 ctgccccagt caataaactt ggatcagtag tgatgtcacg ttttatctgt gttgctaatt
    25741 gattattagt catcttgttt tctcctatgt gttcatgtca attctgcgtc tagacttact
    25801 atttaagtca tctaattcat ttttgtaatc ttgaataagt tgcaaattcc ggtcaatgaa
    25861 gcgttctaca acttgaccta gaagttcttg tgttgtcacg cctctcaatt cagcaagaag
    25921 tctgatatat tctttttgtt tttcagagat ctctgctctt atgaacgact ttcctttatt
    25981 ggtcatctgc gtcatttttt tctcctttag gctagcaatc tcctacatag atccattgac
    26041 cagcgctgaa aatccaatca gctgggtcaa gttcttctcg ttcttcaggc ggttgcatta
    26101 tatctctgtc ataattaaac atgagcatac accttcccaa gctccagaac tcgtttcaca
    26161 tatctagcct tggatgttag cccaagatcc agcaattcgt ttttttcttc atgattggcc
    26221 aaaagccaga cacggttttc aatttcaatc tttgtcatct tcctgctcca cctcttcaat
    26281 tttcacttcg ctatttagac gtttcatggc ttcatctacc gacttgccgc ccaggacgtc
    26341 cttgagcatg tggcttacat cgtgcattgt ttgagccttc gccttgcttc tttcagtctc
    26401 tggcatcaat ccgacatctt gtagagccag aaaggctaaa ctgacatcgt gcatttcttt
    26461 ctgaagctgt ttgatttttt tgattgtttt tagtgcttta aacatattgt tctcctttta
    26521 ttctcctact ttccaaattc ggcaacgtga ctcaatttct ggtagttttt cattttgata
    26581 aacccaatcg ttaccatgaa cacctgatgc gatgtaagat atagatttta agtaatcaat
    26641 cgcttcttct tttgtttcaa aaactcttgc aatatagtct tggtgccccg tcggtaagaa
    26701 atcacgtcca atcaaactga aatcctcgtt tccagtttca gtattcttga cataaatcga
    26761 tataatgtac atctacactt ctccttgcag tctagccttg atatcaaagt tttctttgta
    26821 cttgtaggca gcaagctctt gtttcaaatc gtagttttct tgctcgaaag caaagcgacg
    26881 tttgcgctcc tcaaataggt cggtcatgag ttcgaccgct acctctcgcc agtcaaggtt
    26941 gactgcttta agaactactt caagtctgag ttttaacttg gtaagtaatt tcattaagct
    27001 acgccctcct cgttagattg cttgttcatg cctaaaataa tgtcatagta ggaatgacca
    27061 gcagggatga catagcctgt caaatcatca acttgagagc catctgccat gatgtttaca
    27121 attcttggtt tccattgctc tttttttctc ttcatgttat aattacctcg aatacttttg
    27181 ttgagcgcct gattgccgtc aggtgctttt tgttgtcttc tagactgtct tactttccat
    27241 cgccctgagt tctatctcat ggctgacttg tttcaatagc ttctcacacg ctattttagc
    27301 ttctctgtac gttgtagatt cgctgatgaa gtagtcagca agttctatga ttttatcttc
    27361 cattcaacct cctatatcag tcttgagact gatgtaattc cttcctaaat tgctataata
    27421 ctcttgacta ggacctctca ctgttttagt caaaatttca atagaaagga ggaaaattat
    27481 atgagtaaac ttagcagaaa accaaaccac catgttaaaa aacttacttg gtcagacctt
    27541 gataacattc tgatatctgc ttttacagag tcagcgactg atcatcctag tgcagtaatc
    27601 cgattatctg attatgagat gtctaaatcc gaaattatcg aagaagcaat tgcacaaggt
    27661 tacacagtta ttgacaattc tgatgacttc ttagagtttg aatagcgtgg gtcaaatatg
    27721 atatgttagt actatttgct tcaacatatc tttttaattt agcaatctgt tcatcggatt
    27781 gccttttctt tcttcctgaa tacggatatt ttcttggtct catttcctca cctcctttgt
    27841 atttattttt tctaccctct cttttattta tttagagaag taggactggt tgtcttttaa
    27901 tatttattgt tatttaatac ttgttgttag ttaatattta ttagtgccta aattttctga
    27961 tttgtaaaat acagatttgt aaaatacaga tttgtaaaaa tcggaaatgt aaattctaac
    28021 ctgtggataa cttagatata ctttcattca atctctgttt catgatttca aattgaaaat
    28081 cggatatttt tacatctgag aaaaatctga aaacacgaac tcctttacca cgtcccatgc
    28141 cttttttaac aattcgtagg tagccatttt tttctaatat tttgaagtag ctatcaactg
    28201 tgtctcgact aacacctttt cgtttagcta tttcatctgg atagacttgc caatttgggt
    28261 ggttagctag gacaaccatc ataataccaa cagctgtaaa gtccagtttt gggtcattga
    28321 taaaactatt actaacagca gtataatttt cagtcgcatt cttgaaagat aaattgacaa
    28381 tctaaatttt taaagtctgt catacgctct cctttctttt tttgtttaat ttgttaaaca
    28441 ttttctttaa aaaaaatctt tcacttgctt attaaaaact actgctaatt tttgaagtgt
    28501 tcgaattttc actgttgacg actgacctga ttcaattaag tgtattgttg ttcgagaaac
    28561 atttgacttc tctgcaagtt cctcttgaga cattttctct ttttcacgcc attttcttaa
    28621 acgttctcct tgcacgcact cacccccttg tgttaatagt tgccctgtcg tttctagata
    28681 gattgttcta atcattgaca ggcttggctt ttaattcaaa ttcaataata ctaagtcgat
    28741 cgattgcttc ttgcaattct tcggcttttt ttgatacttc tttacaggct tcctttagtt
    28801 cttcaatacc cgaaacttca acattaagcc gatatcctat tggtctcatt tctttctcct
    28861 ttttgatata atgtttaata aaaacgaggt ttgctatgtt aagtattgat acacaatttg
    28921 tagatacaat cagtaaaata ctatctgatt atgtttcaca ttctgaaatc acaaggatgg
    28981 gagaagtttt aggatatccc caaaacgacc agaactctgg actcaataaa caccatagag
    29041 ttcacaatat catgtctgat atactcaaca aaacacaaga taaatcaaat atcaaacttg
    29101 taattgagta tatctgcaat cctttgaggt acatcgatac ggtttcagat tttgaaaact
    29161 taagattaaa actaaatgtc gttctttccc taaaaggcct taccatatca gatgacggac
    29221 atgtagttat tactactgct tcacaaactt tagttgaggc aaagaaacga tttgaatcac
    29281 ttgaacatat gttgagaaca ttaaatgttc atccaaatgt tttaaaattc tgcacccagg
    29341 aactcttaca agaaaattat tttcatgctg tatttgaagc aagtaaagga atctttcacc
    29401 gcattcgttt gctaaccggt tcgtctctgg actcagcaag tctaatagac caatgtttta
    29461 aaaccaaaga acccatcatg attatcaacg gaaataaatt acaaactctc gacgaacaaa
    29521 gtgaatataa aggattgaag aatttacttc tgacaatcgc acatctttat cgtaattcca
    29581 aagcacataa acttaaatac tacaatccag atagcgttca tgatgcctta actgccttaa
    29641 ctcttatgtc cctcgctcac aatctccttg acaactgtac taatactagg agacttgatt
    29701 agtagattat aaaattctat agtcacctca gctaatctaa ttgtttcttc atctatggga
    29761 ctattgtagt cctcaaggtg atgaagcctt tcagtcaact gctctgataa gtattctgtt
    29821 tgcctaaaga tagatttatg aagatgagta attggtttta gcaacacgat ttcatcataa
    29881 gttaatattt tttcagtctc ctcttgagtt acttctgcta atttttccat accagaaata
    29941 tcaacattaa catgcggctg ttccattatc gttccccctc gtcttacttt ccagcgccct
    30001 gagttctatc tcatgactga cttgaagaaa tagcttctca cacgctattt tagcttctct
    30061 gtacgttgtg ttttcgctga tgaagtaatc agcaagttca atgattttat cttccaattc
    30121 taactacctt tcaaatgtgg tataatcaaa ataaaacgat tggagaaatc ttatgaataa
    30181 ttttagtttt atagaaacac ttgcgatcgc tgccatacct gcctttgttt ctggcatgtg
    30241 gtcttacatc gctgctaaag gaaacagcaa gcatgaaatt gataaaatta acattgcaca
    30301 ctcacaagag cttgaaaatg tcgaaaatca atttaaacaa gatatggaaa aaatgcaaaa
    30361 gcaacactca caagaacttt attcgctgca acaaactcac gaattaagat tacttgaact
    30421 tgaaaaagta tctcaactag acactcaaac cgaccaagga ttaaagataa atgacctaat
    30481 ttttaaagct atttcaggag aaatatctgc agatgtagca ataaaaaata taagcactct
    30541 tagtcatttc gcgaacaagc aacaaccttc cgatcttcaa aaacaattta tgaaaaaatt
    30601 atccaagaaa aatcacaaat gatacttttt cttgatacgc tctatctcag aatcaaacca
    30661 cattttgtgg tttttaattt tctttttcag atgatgctta ccagctaagt aagcaattaa
    30721 attcgtgata aaactagcaa tcattgatat gcctagtatc gtaagaaaag attcattcat
    30781 cgttctactc ctttctctct tttttcgctc tatgagcaac aacctgccaa ggattcgaac
    30841 cttggtgata ccaatcaggc tacattcatt ttatcaagca ttcctgcgaa cgctgcatcg
    30901 aaacgaatgt catcgatttc ctcttgagtg aaaccagaat cgagaaggta acgctcttgg
    30961 cgttcaatct catctgctaa ctctgtccat ccaaaagtga attgacggca gttagtactg
    31021 aatgtttcaa gttggctgta gaggaagttt tcctcgtaag tgccttggat taaagtttcc
    31081 ttagctactg ctttaaagat gttgattgct ttctcgttta atgtgttcat ggtgtttccc
    31141 tccggtttgt ttttgttatt tccttaagct tgattatagt ttaacacgtt aaacacaaaa
    31201 tgtcaagtgt gttaaacaaa aaaatttact ttttttattt aaagatgtat aatggattaa
    31261 acaatatata gaaaggggtt ttaaatgaag ttaggagaat tgctaaaatc atatagaaca
    31321 gagcataaat tatcaatgga tgctttttgt gaattatctg atttaacaaa aggatatatt
    31381 tctatgcttg aaaaaaatga acatccgaaa tcgaaaaagc ccattgtccc atcttatgac
    31441 acaatagaaa aaattgctaa aggaatgcaa atttctacag aagatttaat tgatatgctt
    31501 gatgatgatc aagaaattca aatcaacgct actccagctc ttctttcaaa atccccaatc
    31561 caaaccatct acgacgaact agaaccacct agacagggca aagtcctgaa ttatgcaaag
    31621 aggcaactgg acgagcagga aaacgaagaa gaaacgaaga taaacgaagt atcggagaac
    31681 atcatcagac tggacgacta cagacagact acttaccgac gtgttactgg ggttgtctct
    31741 gctggtagtg gttcgataca ggacgacgat ttagatatgg aggtttcgtt ctatgaggat
    31801 gaaatcccag acgactacga cgctatcgct tatgtcgtcg gcaactctat ggagccaaag
    31861 ataaagaatg gtgactatct ttttataaag aatacccaac aggttgatta taacactatc
    31921 ggcatcttcc aagtagacgg cgctaactat gttaagaaac tgcgtcaggg atatctggaa
    31981 agcttgaatc cagattatga ggatatacac ctagacgaaa gcaacgacat ccgaactatt
    32041 ggggaagttg tcagtgtgta tagggagaaa taacatgagt aacgaaagta gaccgatgga
    32101 agtgattaaa cacaacctag attgcaaatg ccacagacga agagagtgga ttagagtaaa
    32161 tgataaatgg catgctatcg agttttcggt agacgatcca aacgaacctc ctatgacaga
    32221 ggaagagaaa gccaacgtgg ccttaattct tcaacaacac ttatcgaaag aataaaacca
    32281 actgtttcca aaatggaaat aattgcaaac aaaaaaagcc ccacgctctc aaaactttgg
    32341 cgagtctgag cgtgaggcat gtgacaggaa aagattttca tggagataac ctctcatgat
    32401 gtcttttctt gtacccattt tatcattttt taggaaattt tgaaagaggt actactatga
    32461 taacaacaaa taaagtagct atatatgtca gggtatcgac gacaaaccag gttgaggagg
    32521 gatactctat agatgagcaa aaagacaagc tctctagcta ctgcgacatt aaagactgga
    32581 atgtatacaa agtatatact gatggaggtt tctcaggatc caacactgac agaccagcgc
    32641 tagaaagtct tatcaaagac gctaaaaaaa gaaaatttga cacagttcta gtctataagc
    32701 tggaccgtct tagccgtagt caaaaagaca cgcttcactt gattgaggat gtattcatca
    32761 agaatgggat tgaatttctg agcttgcagg agaactttga cacctctact ccttttggta
    32821 aggctatgat tggactcttg agcgtctttg ctcagctaga aagggagcaa atcaaggaac
    32881 gcatgcaact tggcaagcta ggacgtgcca aatctggtaa atccatgatg tgggctaaga
    32941 catcctatgg ttacgattac cacaaagaga caggcacagt gaccatcaat ccagctcagg
    33001 ctctgaccat taagtttatc tttgagagtt acctgagagg gagatctatt actaagttga
    33061 gagatgatct aaatgagaaa tacccaaagc atgtgccttg gagctatcgg gcggtcagaa
    33121 ccatactcga taaccctgtc tattgtggtt tcaatcagta taagggagaa atttatccag
    33181 gtaatcatga gccgattatt tcaaaagagg aatacgataa gactcaatct gagctaaaaa
    33241 taagacaaag aacagcagca gagaatgtca atcctagacc attccaagct aagtacattt
    33301 tatccggtat cgcccaatgt ggatattgtg gcgctccttt aaaaattatg ttaggcgtaa
    33361 agaggaaaga tgggagcagg ttaaaaaaat atgaatgcca tcaaaggcac ccacgaacgc
    33421 tgagaggcgt tactacctac aacgacaata aaaagtgtga ctcaggattt tactacaaag
    33481 acaagctaga ggcctctgtg ctaaaagaaa taagcaagct acaagatgac gctgattacc
    33541 tggacaaaat attttcagga gacaatgctg agaccataga ccgtgagagc tataagaaac
    33601 aaatagagga gctatcaaag aaactgagca gacttaacga tctatacata gatgaccgca
    33661 ttacccttga agaattacag agcaagtcag ccgaatttat aagcatgagg gctactcttg
    33721 agactgaact ggaaaacgat ccagcactca ggaagaacaa aagaaaggct gatatgagga
    33781 aactgctaaa cgctgagaaa gtcttttcaa tggactacga aagtcaaaag gtgcttgtta
    33841 gaaggcttat aaacaaggtt aaggtgacag ctgaggacat tgttatcaat tggaaaatat
    33901 aaataatttt agtaacctac atttccacca atgtaaaagc tttaacctta gcttttttca
    33961 agaatgtcat catttttttc attttaaaaa tttacctcca tattttgata catgggcatg
    34021 agcattgccg cataaagtaa aacgataatc agtgccacaa agataaaaac cagtggctgc
    34081 accaaattca tggtgcggtt gactcgggta aaaaaggctt cccaagtttt ttcagcatag
    34141 atttccaact cactacccag cttggactta acttccccat actctatgat aagactcaat
    34201 tccttcctaa agaaaggata ggttcctatc gtctgagaaa attcacggcc atttttcagg
    34261 gtttgagcca gatcttgacc gatttcttta aagagctggg aaccttgttc ctgcatcatt
    34321 tgaaaaatct gcgtcagctc cattccctgt gaaatcatat tcccccattc acgtgcataa
    34381 taggctgtca agtaggtctg cacaaagatt ccaataaagg gaaggcgtgc taagatagaa
    34441 aagacactca tcttagaact tcttttataa aaagttagtg ctaaaagggc aagcacggaa
    34501 acaagcccta ccatgcctag aaaaatttgt ggcagattac cgataattcg ggtggcaata
    34561 ttgctactat ccagttgtgg gagcaggtaa ttccgtagcc ccagcataat taagagaaga
    34621 aaacccagca aaatcaaagg ataggtcgct acttcaatta actttttctt gaccttggcc
    34681 agattgtcta gatattcttc tatctttccc aaactcaggt gaagattgcc atgaacttca
    34741 gctagagata actgagtgac aatggtactt gaacatccca aactttccat catttctgag
    34801 aatgatttcc cctgagacaa gcctgtacgc atctgggtca cacactgctt gtccaacaaa
    34861 gcactcctat ctaaaaagga gatggtctcc accagatgaa aaccgctaga aaagagattg
    34921 ttaaataggg tgatgatatt tttttgctta gctgtagcta attttttccg tctcagcctg
    34981 aagacttgtg atatgtccat ctttaagaag ctggtcaatt tgctcattcc acttggctgc
    35041 ttggtgttct tgataatctc tgcttgcaaa gtcaacgatt cctcctcccc cgattaatct
    35101 ctggtagcag actccttgca gaacaactgc caattcttct tcactcacac ccaactccag
    35161 cagacgctca taaacacctc ggatactttt ggcgtgaatg gttgaaaaga ctgtcgcacc
    35221 tgtcaaacta gctctgacca ctgcacgcgc cgtctcgctg tcacgaattt ctccgataat
    35281 caagagatct ggtcgatgac gcaaggaaag tttgattaga ttttcatagg ttaggccgat
    35341 tgcttcgttc aactgcaact gaagcatgtc gtcctgcttg atttcgacag gatcttcgat
    35401 ggacataact tgctgtcctt taaagagtga cttggacaat tcatgcatca aggtcgtctt
    35461 accactccca accggaccag caaaaagata gagtccccgt tgcctgtact gcttgcctaa
    35521 ttcttcaata tcctgaaacc aaaaatgcaa gtcctgctcc tcatcgtgca acaaacggat
    35581 aaccaaactc tcatgccccc gataatcgcc tacagtagat aaacgtagag acgctatctt
    35641 ctggtcatag gcataatcac aggaacccag ttgactacgt cttttttctc ccacattcat
    35701 acccgccaca aacttaaagt gactgataac ggctgcaaac ttttcaaaat catagctacc
    35761 aattttacag cgctcgtctc ctaccctcat atgaagctca taggcatcta acttagggac
    35821 aaaataaatg tcttgcgccc cttttttccg agccgaacga atgatttctt gtgcaatttc
    35881 ttgaaccata cttacctcct cacctatact attcgcaaag cgttagaaaa ataaaaaaag
    35941 caactccaaa aaagttactt ttctctattt taatttcata cggcaagaac gattcctttc
    36001 ctgactggtt tgtttttcat agcctgagcg tagtttttcg tctgggataa cctgctcatc
    36061 ctgcaaaaga gccaaagaat ggtattgttg taaatactca tcacattcat cacaccagcg
    36121 ttggtcttcc gggtcccaaa aaatggtcat caagtcgctt ctacttttat aaagagggga
    36181 ttcttgttcc aatctaaacc agcaagtttt gtagtatttg agagcatcat aatactggtc
    36241 aaattttcta cttgctacaa tatcttcttc ccaaccttct aagaaccacc acggttcaaa
    36301 atctccgtac atttctataa cacgatacat attcattcat tcctttgtac cgtatattat
    36361 aaccaattcc gccagacaag gaaagaaata tgctcatttc ttaatttttt gataaaaaat
    36421 ccagccatct gatggatgac tgaatttcta tttacttatg tacaaaaata tttttactac
    36481 ttaggcttct gagatatcgt tttcgataat gaccttaatg gcatggtggt ctgctgcctt
    36541 actgaagact tcgtaggctt tttcaatttc actgagtttg aaatagtgag ttaccaattt
    36601 ttctggttca atcttatgac tttcaagtgc tttcaacaat tgtggagtcg tatttgtaga
    36661 taccaaacca gttgttacat tgatgttgcg aatccaaagt ttatctaaat cgaattcaac
    36721 tggtttacca tgcacaccac agttggcaac cgttccgtct acaccgataa tcttttgaca
    36781 gaaatcaaat gttgcaggaa taccaacagc ttcgatagcg acatccacac cacgaccatc
    36841 tgtcaaatca taaatttctt taatggcttt ttcagggtct gaagaattaa ccttatgagt
    36901 cgcaccgaat gatagggcag tttccaagcg gttatcgtct aggtctacca taatcaattt
    36961 agctggtgaa tagaattggg ctgttaaaag agcagccaat ccaactggac ctgaaccaat
    37021 aatggctacg ctgcaaccag gttctacttt cccttttaag acaccaattt catatccagt
    37081 aggcagaatg tctgacagca taaccaaagc ttcatctgac aagtcttctg gagtatggta
    37141 aagagtatta tctgcatgag ggacacgtag atattcagcc tgcataccat caatcaagtg
    37201 accgaaaatc cagccccctt cgtcttcaca gtgggcataa attccttttt tacagtagta
    37261 gcatttacca caggcacaga cgcaagaaat caagaccttg tcgccttttt tgaagttgga
    37321 aactccttcc ccaacttctt caacaatccc aatcccttcg tggccaagaa tggtaccact
    37381 ttggcaagta ggaacatccc ctttgataat atggaggtct gttccacaaa tagtggtttt
    37441 tacaatacgc acaatagcgt ctgttggctt gcgaataact ggtttgtcta catcaacaaa
    37501 agaagcaagt cctggtttaa cataagtata ggctttcata aagcactcct ccgttttttt
    37561 atttgtaata tttatagttc aattgtaagc ctttttcttt gtttcttcaa gctttttttg
    37621 tgattttttt aactttttat ttaccagtaa agtaccctgt ataaaaaagc agaagctagt
    37681 ctctaacttc tgccttctct cttatgcttg ataacgtttt acgccatcta gataggttgc
    37741 taccaattcc aaatctttat ctaatacgat gaagtcagcg tcgtagcctt cacggatttg
    37801 gccacagacg tcatcgatgt gaacggattt agctgggttg aagctggcca tcatgactgc
    37861 ttcatgcgga ttcgcaattc cccattcgac cacattcttc aaaccatctt tgagtttgag
    37921 gatagaacct gccaagttac ctgtcgattt gaggcgtgca gttccatttg caacgacaac
    37981 tgggaattct cccaacatat aatctccgtc ttccaatccc ccagctgtca tacagtctgt
    38041 gataagagcg atgttttctg ttcctttttg tttgataaga atttcgcaag cctttggatc
    38101 tacgtggtga ccatcacaga tcaactctgc ataggtatgt ggcaattggt acatggctcc
    38161 aaccataccc aattcacggt gagtcaaccc acgcattcca ttgtaggcat gcacccaaac
    38221 actcgctcca gcatcgactg cttttttggc ttcatcaaaa gtcgcgtttg aatgtccaag
    38281 agcaaccgtc acaccttcgc ccgtaactgt acgaacaaag tcttccaccc catcacgttc
    38341 tggtgcaagg gcaattttat taagcaagcc atttgccgct ttttgccaag aatgaaactc
    38401 ctcaacaccc gggtctctca tataagttgg attttgtgcc cccttaaaag tttctgtgaa
    38461 atatggacct tcataataaa tcccacgaat cttagcacct gttgcttctt tataatggtt
    38521 tccaagattt tcagtgactg caagcaattg ctcataagtg gctgttaaag ttgtgggtaa
    38581 gaaactggta acaccggtac taagaagtcc ttcactcata gtatgcaatg taccttcaat
    38641 gttgttgtcc atcacatcta cacctgcata tccatgaata tgagtatcca caagacctgg
    38701 ggcaatgcta taacctgtat agtcaatcac ctcagcccct tcaggaatct gctctacatg
    38761 tttcccaaac ttgccgtcca caagttccaa gtaaccacct cgacgaactc cgtgtgggta
    38821 gaaaaactga tccgctttaa tatagttagg cataatgtta acctccttaa aagattgatt
    38881 ctacaattta ttatgtcaat tacattataa acctttcttt tttatttgta aatggtatag
    38941 acctattttt tggagatatt ttaaaagagc tggatttctc caactcttct cttttttaat
    39001 acaagttatt ttgatttaac cggcttgtct tgagctgtct gcaaagctgt ggcaatcgta
    39061 tctgcataca attttgctcc tgcttcgata gtgctactct cactcccgaa atgaacctgg
    39121 tctgttccag cccaaatttc tggatgctct ttcgcaactt gattccaatc tgctatcgta
    39181 atgtaaggtg tcttctctgc caattctctc atataggcag cagccttctc aacgatggca
    39241 taggtctctt ttgtcttatc tccctcataa ggagtcacca aaatcatatg gtgtccctta
    39301 ggaagatttt tcacgatact gtcccagtca tccttgtaat tctcaggatt atttacccca
    39361 gtcgcaatga ccaccgtctt aggtaaaaat ttattctggc tattatttag catgatttca
    39421 tttgcggtct tggttgttac gctgacctgc gcgttaatct gtgctccagg aagagctgtc
    39481 tgtagtgctg tatttgccct taaagccact gagtcaccaa ttaacatagt gccatcagca
    39541 attcccaaac tgtttgcatc tgcccgttct gccatcacct tggtctggcc aatatttgtt
    39601 gcagcttgct tcaagccatt gacagtcaag tctgtctcaa acgctcccac ttgtggtgcc
    39661 aacaaggtca ccgtgcagac aatgatggtc aagattcctg tacctgctac aagaattgcg
    39721 tgaatataag gcaggggacg aagagtttgt aaaataggtg tgttcttgcc tgcaatccaa
    39781 ggttccaata catagaatga cagactggca aagccataag aacaaatcag agtcagtaat
    39841 acagcaagaa gatttgatgt caactgtgag aaaatgatat agaaaggcca atggaaaaga
    39901 taaaccgcat agctagtatc cgctaaaaag ctgataatct tcggctcctg tatatgatgt
    39961 gtcttttcat gtaagacacg cgccgccaga atcatggcaa gagctgcaag actggcaagc
    40021 aagaagccga taagataggc aaaaagataa gtgaatttga caaagaaagt caaaagaact
    40081 aggaaaccaa agcctcctcc aaaaactacc aaagtctttc gtaaatccca gattttatcc
    40141 aactgcttga cgagggaagt cgtctgacga acacctacaa tagttgctaa catacttccc
    40201 aaaaagaatg gatagacatg agttaaactg gagaaataaa cagaggaata agaggtcact
    40261 agaaaactac caataaacat ggagaagaaa ctgatcaaga aggcagcagc agataagaga
    40321 aagaccatcc ccttcaactg accatttgat ttagcttgtt tggataagaa ccaaactgcc
    40381 aatccccaaa gaatatagta gtgaacctca actgccaaac tccaattatg aacaaacaaa
    40441 tgaggaatga actgagattc ataactccca cctgttagga gttcatagaa gttggtcata
    40501 aagcctaaga cgcccgcaat ctggccacca attccagcaa catagtcttg gcgaaccaag
    40561 aaagtaaaag gcatggtcac caagaccatc aaaaccacag gtggcacaat ccgataaaag
    40621 cgtcttctaa aaaatcctat caaatcaatc tcattgttct tagaaaattc ttcaatgagt
    40681 agagctgtaa tcaggaaacc tgaaaatgtg aaaaagacat ctaccccgaa aaatcctcca
    40741 ggaaagatcg tctgaaagaa gtgatacaag agtaccaaaa gtaaacctat aatcctaatc
    40801 aaggaaaacc atttaatgcg catacgagtt tattcttcct ttcattgtac actttattct
    40861 atcaaaaaaa gaagaaaaat cctaagagaa aacttaggat ttttttatat caattccatt
    40921 ttagaaatta cgtcctgact tattatagcc atatttttcc acaaaatact cacggaattc
    40981 caagagattg tcatccatga tggcttgtcg cacttgcttc atcaggttaa gcaagaagta
    41041 aagattgtgg tagctagtca agcggatacc aaaggtttca tcagccttga gcaggtgacg
    41101 aaggtaagcg cgtgtatagt tattacatgt gtagcagtca cactcaggat ccagtggcgt
    41161 aaagtcctca gcaaactggg catttttcac aaccaaacgt ccttgactgg tcatacaagt
    41221 cccgttacga gcaattcgag tcggtaagac acagtcaaac atatccaccc cacgaatgac
    41281 cccatcgatc aagctatctg gcgctcccac acccatcaga taacgaggtt tattttcagg
    41341 tagcagttga gttgtaaagt ccaagaccgc attcatctct tcatgggttt ctcccactgc
    41401 caaaccaccg atagagtagc ctgagaaatc catgctgaca agatcatgag ctgattggcg
    41461 gcgaaggtct tcaaatcctg caccttgcac aattccaaac aaaccttggt catgtggacg
    41521 acggtgagcc ttcaaaccac gctcagccca acggctggta cgctcgatcg atttcttaac
    41581 gtagtcataa ggttgataaa actgaggaca ttcatcaaag gacatcatga tgtctgaacc
    41641 cagattattc tgaatagaga tggctttttc tggggatagg aacatcttag aaccatttag
    41701 atgattttta aaggttactc cttcttctgt gatattacgg ctatctgcta aagaataaac
    41761 ctgaaaacca ccactatctg tcaagatagg ctggtcccaa ttcatgaact tgtggagacc
    41821 accagcgcgt gcaatgagtt catctccagg gcgaagccag agatgatagg tgtttgatag
    41881 gataattccc gaacccatct ccttcaattc ttcaggtgac tgagttttga cagtggcttg
    41941 tgtcccaact ggcataaaca taggtgtcgg aaaggtaccg tggggagtga tgatttctcc
    42001 cagacgagct cctgtgtgtt tttctttctt aatcaaacga tatttgattg gtgaatctga
    42061 cattttttac ctccgaagct gggaaagaca gtcccagttc atacagtgtg cccaaggaca
    42121 cactgtatga ttttatcaaa aaaagtagga actgtcacga actatgtata agaactatgg
    42181 tataataaga gaatgaacta tccaaaaatt gatttaaaaa caattcgtca ggagtcaaaa
    42241 cattttcagg ctgacacccc ccgtctcttt ctcctctata tccttcccag tatgctggtc
    42301 atcttatctg gttttcttaa tcccttatct cggatccacg ggactgtttt agaacagcca
    42361 tttttcagca tacttggcca aatacttcaa acctacctct ttcctctact agtctccttt
    42421 atcgggacta ttcttttgac cagttccgtc tatgcaacac tgacactcat gaaagattct
    42481 aaaacggagc catctgtaaa aaacagccta gctctctttg atgaggagcg cttttctcaa
    42541 acctttttga ccctacttct caaacgtttc tatctctttt tatggagtat ccctaattta
    42601 cttgggattt acctcctctt ttatagtagc tttttagcca aaaaattcgt caccctacat
    42661 cctgagtttc caaatctaga tctctcatca gttgaaaccg agcgtttcct catggtcttt
    42721 ggactctact ttcttgcaag tctcatcttg atcattgtag gaaatatcct ctatattcca
    42781 cagtattatg cctattcaca ggtggaattt cttctctgtt atagtctaga cctaggacag
    42841 gtcccaccaa gacgaatcct aaaaactagc cgttccttta tgaagggcta caagttccag
    42901 cactttgtcc tagatttaca gcttcttcct tggtacttcc tcaattggat tacctttggc
    42961 attgctagtt tttcactcct accttatatc caatgcacta aaattatgtt ttaccgagca
    43021 gtattggccc gaaaacgtcc aaaagcttga aggtttttcc cctcaagctt ttttctatca
    43081 atgagtttct ccgcctacca acttgagttc ctgatctcca tgttctatta tagcgccgat
    43141 tgctgcttct atccctcgcc gaatatccac taaactcata gctggagtag tcggtctgtt
    43201 caccacctgt tccatcatat aaggaatatg cataaaacct gccttaacat atggagattt
    43261 cttttctacc aaatagagag cctgatacat caaatggctg cagacaaaag tccctgccgt
    43321 attggaaaca gaggccggta agccctcttt ttttatagct tgaaccatcg ctttaatcgg
    43381 caaactacta aagtaggccg aagcaccatc tgggcgaatg ggacggtcaa tcggttgatt
    43441 atcttcgtta tcagaaatgc atgcatcgtc ttgattaatg gtcactcgtt caggtgtcaa
    43501 actagttctt ccaccagctt gcccaataca aaggacaaag tcaggttgat aacgattcat
    43561 ctcttcttcc aatacttgag cagatttgtg aaaaactgtc ggcacctcta gccaacggac
    43621 ctcagcacca tggatttcag ctggtaaacc tttaatggcc tccaaagctg gattgccctt
    43681 ttcccctcca aagggctcaa aacccgtcac taatactttc atttctaact cctcaaaacc
    43741 aattcaatga gacttttttc ttaaaaacaa gtataacata tttcccttat tctggattaa
    43801 aaaggattag cgagattaga atcgaccttc aagtgtattt aaagagtaaa atactaatca
    43861 aggccaaaat agctgatcca ccttgtttca aaataatttt tttatccgct gttaaagagc
    43921 cgtaagtcgc agcaccaatc acaaataaga caaaaatagt cacaatttct aaattctgtg
    43981 agaaataaat gacatacaag agaaagactc ctagcagagc cttataaatt ccttgatttt
    44041 tgaacaatga acttactgac ggatgagcca attcttcctt ttccatatta aatacacgac
    44101 tagtcgcatc tgattgcgtg gcaatacttt ccaaataaaa aatgtaaaaa tgctccaaag
    44161 caacgatcgt tgttaaaata attgacatct atttctcctt aaatttctat attttcaata
    44221 cccaaaacca atttatttaa taaacggctg agttctgttc tctcaaactc tgttaagata
    44281 ctttctatct cttccttaac cctgatatgt tgcagagggg gattcaccac taactgctcc
    44341 ttggcatact tcgcagcctc taccaacact tcccgctgat tttcaggatt acgatgacgc
    44401 tccaccaaac cttccgtttc caaaattttg aaatgccgtg tcaaagcagc ctgatcaatt
    44461 ttcaaacgct cctgaaccgc catttggtta caaggagaat gctccagcaa aaacagtaaa
    44521 atctgatacc gtgtcaaact aatcccaagc cttttttcaa acaattgcgt actcgcttgc
    44581 tcagacaagc ggagttgata cagtaaatct tgaatctcta gcatttcttc ccctctcttt
    44641 ttattgattt atcaatattt gactaattaa ttgtgatata aagtaaaata gattgcaaga
    44701 atgttgctta actcattaca agatgataac tttccatttt ccattcaaat tgatactcaa
    44761 tgaaaatcaa aaagcaaact aggaagctag tcgcaggttg ctcaaagcac agctttgaag
    44821 tttcagataa aactgacgtg gtttgaagag attttcgaag agtatgacaa agaaaaaacg
    44881 aacgagaatc gctttccatc acgaaagcta gatctcattc gtcaaaatga tgttatcaag
    44941 tcgggtttat ccattcattg aaacgtgaac gtgatcatag tgattttctg tcacactacc
    45001 acggtctggc attgggttcc aagtgttagc tggcccatat ttgctatcga atggagcata
    45061 gaaacgttgt ttccagatga tgtaactaat gccacggctg gccatatttt gaatagcata
    45121 ttccgcaatc ttatccccta attctgaacg ttctggtacc ataaagtcga tagccaaacc
    45181 ttttccgtga tctccactgt ctcctggacg ataaccacta aaggatgtaa tgccaaacaa
    45241 gttagcaatt tcttctttaa aggcagctgt ttgtggttga agacctgcat tttcagattt
    45301 tgctactgca agtccagcat aatcgggcgc agctggagcc tcgtaagttg ttgaaattat
    45361 tttcgtctct tctggttgat aagtagaaac tgctgctgta gctccatttg atgatgctac
    45421 ttcttttgct tctgaacttg ttgtagttac ttccactgct gatgcagctt gagggcttgc
    45481 ttgtgtttcc tgcttctcag ctggagtcgt ttcctgagga gttgcttctt caactgggcg
    45541 agttgtttcg gtcgtttgct cctctgggac agaagtgccc gtagatggtg ccacttcttc
    45601 agaagcaatc actgtctttg taacttctgc aattggttga gaaaggtctg caacctgaac
    45661 agtttgatca tcaacggtca cttgattagt ggtcaaatct gctgtcgcag ttgtcacttc
    45721 ttcactagag tctgcttgag gtgtttggat ttcaacttct gttacttctt ctgcttcatt
    45781 gacagtcgtt gtcaaaacag tttctgggaa aatcaagtcc atattagtga ttttgttcag
    45841 attcgcaagc actgttacat ctacacccaa ggcttctgca atggtgctca aagtatcacc
    45901 atactgtacg gtataacttg ttttgttgtc cgttttagtc aaatcgtttt ggatttgctc
    45961 aacactacgt gcagtccaaa gaacttcttc tgcttgagtt gccaatactg gggcaaatga
    46021 caaggctact gttgacgcta ataacattct tttcttcatt ctttcaaatt cctttcaaat
    46081 gagtacctgt ctatcataac acaaaaaatg tagaaaaaaa gccctctcgg gcctatttaa
    46141 cagaactgtc tattccttgt aacaaactag gttacttgaa atagtttgtt aggtctctgt
    46201 cacaaaactg acacaatctt tttatcactt agtctcaaaa atacgggaaa tatctgctaa
    46261 cgcttgaatc ctgtgattcc cttcataagt agactctaaa aagttgatgc tttgaatccc
    46321 actattctgg gcaaattcca catccagagt ccgatcccct atataataag tattatcaga
    46381 attcaactga tacttatcta gcagataggt agccgcttct ggacttggct tccgcacaaa
    46441 gccactctga ctggttaaaa tctctgtaaa ataggattcc acccccaagt ccttgagaat
    46501 ggtaaaagcg ttgttcccct tatgagtata tataaactgc tgaattcctg attcgtctgc
    46561 ccaagctagc acctcacgcg cacctggcat caaaactacc tgagcattct tctcagccag
    46621 actctgggca cgcacctgat ttagcacctc aacatccaga tttctatctt ctgccacccg
    46681 cacaagcaaa tcttgcaccg aatacttgaa gataaactct ctcaccttct ccttatcata
    46741 aggaatagaa aactgagcaa aagtctcctc aatccctgat aaaatcgctt cgtaagagtc
    46801 caataaagtc ccgtctaaat cccaaataaa agctgttttt tgcatttcct atcctttcaa
    46861 gctcatatag cgctggtaac gatagcgtag aaataaccag cgaaaaccat tatccaagag
    46921 agaccctgcc caaataccag gcaagcccca accaagcaca atccccatca gatatcctgt
    46981 cccaatgcgg atacaccaca ttcctatact tgtcgcataa aaagggaggc gtgcatttcc
    47041 taatccctgc cagactgccg tatagatgac tgttcctgtc gtcatagggg tcccaagtag
    47101 tgaaaacagt gtcactagaa cactagcctc caccgctaga gaatcagtcg tatagagatg
    47161 agttaatggt acacccaaga catatatact aaaggacagg ggcaacatga ggaacagaga
    47221 aagccaaaag gtttgtttac tcaaactagc aactcttttc caatcatcct ctccaactgc
    47281 tcgggccaac agcatgaccg ttgccgtagc gacgccaaag gcaggcatat agttaaactg
    47341 ggtcaagact tctccgattg cattcccagc aactgcctcc gtcccaaaag aaacgaccaa
    47401 ggcaatgatc actacatctc cagccctcat cataagtcgc tctccagctg ctggtaaagc
    47461 caaggtcaac agttccttat ctaaaccaaa agttggcttc ccataaggca gttttaattg
    47521 tgaccacaaa atcacaagac caaccaaacg agacacaatt gtcccccaag caacaccagc
    47581 tatccccata tccagaacaa aaatagctag acttgaaaaa agaatattca aggcattgga
    47641 taaaaaacta acatagagag gcagacgtgg attatgcgtt gcacgaatca aggctcctag
    47701 actagtcatt aaacctaaga gaacaatcga tccgcctacc aaagatagat acagtccacc
    47761 actctcagct acatccctct ccgtccccaa aagtcctatc atctctttcc cagcgaagat
    47821 ggacaaaaat cctaaaagga aacttaatag taaggtaatc ttcaacgcct cagtcacatg
    47881 ataggccaac ttcgactggt ctttctgccc tatgcttttt gaaataacac tggaaatagc
    47941 agctcccaga gcgatgaaaa tcgcctgata aatggtgata atattaccag ctactgaaac
    48001 ccctgaaata gctatcaatc ctaaatgagc aaccaaataa ctgtccacca ttcccattag
    48061 catctgcaaa aagttttcac ccatagctgg caatgcaata ttaagaatgt ctttattttt
    48121 cttaaacaat ctctcctcct gatgaaaaga aactcagttg gtttcccaac cgagtttgct
    48181 ccctctgtct taaagtccta aataagcctc aaccgctgct tgcatgtcag cagctgccac
    48241 tgttgtcttg tgacgaattg gagctatttc aagcccatca actgctggtg gcactgcaac
    48301 gcctgagatt tcatgtaatt gagccaaggc ttcaaagtct gttaaacctg cttttccagt
    48361 tacagcttct actgcaacta ctgggaactt gtatggacta gctgttgaag caatcactgt
    48421 cttagttaca tctccagtgg ccgattggta ttttttataa actgctgaag caacagctgt
    48481 atgagggtcc tcgatataag aatctaactc acaaacacgc ttgatctctg ctgccgtttc
    48541 ttcctcagtc gcatattcag ctgcaaagag gtccaaaatc tctgcatcaa agtctgtcaa
    48601 cttatattgt ccttgcgtgt tcaaggcatt cataagttca gttgtctttt cagcattatt
    48661 tcccaaaaga tggaaaatca agcgctccaa gtttgaagat accaagatat ccatagatgg
    48721 gctggttgtt accttaaact cacgtttttt gtcatagaca cgtgttttaa agaagtctgt
    48781 caaaacattg ttgtcatttg aagcacagat taatttacca actggcaaac caatttgttt
    48841 ggcataaaag gcagccaaga tatttccaaa gtttcctgtt ggtactgtga agttaacctt
    48901 ttcaccagct acaatttcac cagtcttaac caattgagcg taagcataaa cataataaac
    48961 aatttgtggc accagacgac caatgttcat agagttagct gatgaaaatt gcaacttgtt
    49021 ggtagtcaat ttttcacgaa gagccacgtc gttaaacatg tgcttcacat ttgtttgcgc
    49081 atcgtcaaag ttaccatcaa tagcaataac atgagtattg tcgccagtct gagtggtcat
    49141 ttgtaactct tgaatcttgc tgacaccatc ctttggataa aagacgataa tctcagtacc
    49201 aggcacattc gcaaaccccg ccatagcagc tttccccgtg tcaccagatg tcgctgtcaa
    49261 gataacaatc ttgttctcca aaccatgttt cttagcagca gtcgtcataa agtatggcaa
    49321 aatagacaag gccatatcct taaaggcaat cgttgaacca tggaaaagtt ccaaattgta
    49381 ttgcccatct aatttcacta atggtgcaat agctggagta tcaaatttgc tatcgtaggc
    49441 attgttgata cagtagtcca actcctcaac tgtaaagtca tctaaaaatg ctgacaaaac
    49501 tagcttagca acttcctggt aagaagcatc tttcaatttg tcaaagttca aatctacctt
    49561 tggataagta tccggtgtaa acaaaccgcc gtccgtcgcc aaaccttgca aaattgcttg
    49621 gctggcagtt actgtattgt tggcatcacg cgttgattga taaactaatg tcattactct
    49681 ctatccttta tctatagtct cgtccattat atcatgattt ttcagaaaat tctcccttta
    49741 taagagaaat tataggctca attcttctct caaaggctgt aaataggtca tttcttcctt
    49801 atttctcacc actagtccct cctcagctag taggagtaag tctttggaaa attcaataat
    49861 cgtagtttct tcctcatctg taagattttt cttagaaaat tgtctcctta aagacttgta
    49921 atcataacca aatactttaa agaaaggtgc tgtttctaag taagcttcta acttgtctaa
    49981 attaaccaat aatcccaagt gaaaagctgc agaagcaaaa gtcctatcaa gtggctgtgt
    50041 acacacacta cgaaactcaa ctgttcctcg agtcgttaaa tcttggtact ggtaactacg
    50101 atgagtttca aaatccttct cttgggggta aataataacc tcatccccat tcagagcaaa
    50161 tgcttggatt tcggacgtag ccaaatagtc cccagcctga ataggataaa aataataggt
    50221 ctgcccatca cgttccgcag taaaaatcgc agaatgattt agatagtcaa aaaaatcagc
    50281 ttcatcatta aggagtctag cattgacccc aacattctct ggatagatac catgcataga
    50341 ttcttcccag aaaatatccc ttgaaatttt cgtatcccaa tccgcacccg aaaattcaga
    50401 gtttgcaaat aaataagcct tagccgcttc aatttgagta aaagcattaa tcacccgtaa
    50461 gtagttggtt tttgaaatat ccagctgaac ctggctccca cagataaaag taccatattc
    50521 agggaaatga tgtaaatctg atttaataat atttctactc aaattcaaat aatccatcaa
    50581 catctgatag cgtggataag ccactggaca attctcattt ttatcccagt tgggatggat
    50641 accacagcca acaatagcat gatttgattc agctaacttt ctctgaatta cattcatata
    50701 attattgaaa cgattttcga cctcttgaat cgtttcagcc ttaccaaatg caaactcaat
    50761 cgttgtatag gaaacttcaa ataaaatagc atcctgactt atcggatcta ctaactggat
    50821 cagattgcca aaatcatcta cctttgctac ggtgagatcc aaagtagaaa ctaaataatg
    50881 aaatagatcc ttcataactt caacatctgt agcatcccct tctaaacttg caacaggata
    50941 ctccaactca attccgacaa acaattcagg attttcttta atatttttca aataccgatg
    51001 ttttaataaa tcgatagaac aagacatact cccctacact ttcttaatct aaataaaatt
    51061 tgaataacta taattatacc aaaaataata taaaaaagga acgaagaata acaacgttcc
    51121 taacactata ctataccggc ggccggggtc gaaccggcac gtccttgcgg acactggatt
    51181 ttgagtccag cgcgtctgcc aattccgcca cgccggcaaa tagtaactgg ggtagctgga
    51241 ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaat
    51301 aatataaaat aggcgagtga tggggatcga acccacgcat gccagagcca caatctggtg
    51361 tgttaaccac ttcaccacac ccgccataat tctattaaca cgggcagtag gaattgaacc
    51421 cacactgaag gttttggaga ccttagttct acctttaaac tatgcccgta aaatggaagg
    51481 ggagggattc gaacccccga acccgaagga gcggatttac agtccgccgc gtttagcctc
    51541 ttcgctaccc ttccaaaata tataaatggc gcgagacgga atcgaaccgc cgacacatgg
    51601 agcttcaatc cattgctcta ccaactgagc taccgagcct tattgcggga gcaggatttg
    51661 aacctacgac cttcgggtta tgagcccgac gagctaccga gctgctccat cccgcgttaa
    51721 tataaaagga ggatgtggga ttcgaaccca cgcacgcttt tacacgcctg acggttttca
    51781 agaccgttcc cttcagccgg acttgggtaa tcctccaaaa tagtttatac gggaacaagc
    51841 ccgtgtcctc ttagtgaaca gataatattc caatccccta gtaatggact aaacactatg
    51901 ggcacgagtg gactcgaacc accgacctca cgcttatcag gcgtgcgctc taaccacctg
    51961 agctacgcgc ccaagtcaaa aacttggtac aaagaacaaa gttcaaagcg ggtgacgaga
    52021 atcgaactcg cgacaacagc ttggaaggct gtagttttac cactaaacta cacccgctaa
    52081 a
//
LOCUS       DAVKLX010000008        48234 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKLX010000008 DAVKLX010000000
VERSION     DAVKLX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48234
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.008"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            213..527
                     /locus_tag="VW325_000545"
     CDS             213..527
                     /locus_tag="VW325_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733992.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            537..743
                     /locus_tag="VW325_000546"
     CDS             537..743
                     /locus_tag="VW325_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9733993.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            743..940
                     /locus_tag="VW325_000547"
     CDS             743..940
                     /locus_tag="VW325_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733994.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            943..1677
                     /locus_tag="VW325_000548"
     CDS             943..1677
                     /locus_tag="VW325_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9733995.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            1689..2330
                     /locus_tag="VW325_000549"
     CDS             1689..2330
                     /locus_tag="VW325_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9733996.1"
                     /translation="MKLLKNLGWILLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYVSRKGLVYSISLHALNNLVGFLMILML"
     gene            2348..2578
                     /locus_tag="VW325_000550"
     CDS             2348..2578
                     /locus_tag="VW325_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9733997.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            2614..2694
                     /locus_tag="VW325_000551"
                     /pseudo
     CDS             2614..2694
                     /locus_tag="VW325_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2975..4738
                     /gene="aspS"
                     /locus_tag="VW325_000552"
     CDS             2975..4738
                     /gene="aspS"
                     /locus_tag="VW325_000552"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9733998.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            4716..5657
                     /locus_tag="VW325_000553"
     CDS             4716..5657
                     /locus_tag="VW325_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9733999.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(5778..5858)
                     /locus_tag="VW325_000554"
                     /pseudo
     CDS             complement(5778..5858)
                     /locus_tag="VW325_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5994..6980)
                     /locus_tag="VW325_000555"
     CDS             complement(5994..6980)
                     /locus_tag="VW325_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9734000.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(6990..7790)
                     /locus_tag="VW325_000556"
     CDS             complement(6990..7790)
                     /locus_tag="VW325_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9734001.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNIYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDSNYHK"
     gene            complement(8043..8885)
                     /locus_tag="VW325_000557"
     CDS             complement(8043..8885)
                     /locus_tag="VW325_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9734002.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(8887..10179)
                     /locus_tag="VW325_000558"
     CDS             complement(8887..10179)
                     /locus_tag="VW325_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9734003.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(10290..11561)
                     /locus_tag="VW325_000559"
     CDS             complement(10290..11561)
                     /locus_tag="VW325_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9734004.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            12111..13628
                     /gene="malQ"
                     /locus_tag="VW325_000560"
     CDS             12111..13628
                     /gene="malQ"
                     /locus_tag="VW325_000560"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9734005.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            13654..15912
                     /gene="glgP"
                     /locus_tag="VW325_000561"
     CDS             13654..15912
                     /gene="glgP"
                     /locus_tag="VW325_000561"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU9734006.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(16957..17529)
                     /locus_tag="VW325_000562"
     CDS             complement(16957..17529)
                     /locus_tag="VW325_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734007.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALEGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(18374..19222)
                     /locus_tag="VW325_000563"
     CDS             complement(18374..19222)
                     /locus_tag="VW325_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9734008.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSKLTEITIEAEI
                     LVGKAF"
     gene            19374..19652
                     /locus_tag="VW325_000564"
     CDS             19374..19652
                     /locus_tag="VW325_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734009.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            19654..21717
                     /locus_tag="VW325_000565"
     CDS             19654..21717
                     /locus_tag="VW325_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9734010.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRICVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(21795..23051)
                     /locus_tag="VW325_000566"
     CDS             complement(21795..23051)
                     /locus_tag="VW325_000566"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9734011.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            23193..25658
                     /locus_tag="VW325_000567"
     CDS             23193..25658
                     /locus_tag="VW325_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9734012.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLRENGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKAGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            25723..26634
                     /locus_tag="VW325_000568"
     CDS             25723..26634
                     /locus_tag="VW325_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9734013.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            26864..27562
                     /gene="dapD"
                     /locus_tag="VW325_000569"
     CDS             26864..27562
                     /gene="dapD"
                     /locus_tag="VW325_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9734014.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            27630..28760
                     /locus_tag="VW325_000570"
     CDS             27630..28760
                     /locus_tag="VW325_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9734015.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            28772..29311
                     /locus_tag="VW325_000571"
     CDS             28772..29311
                     /locus_tag="VW325_000571"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9734016.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            29295..29972
                     /locus_tag="VW325_000572"
     CDS             29295..29972
                     /locus_tag="VW325_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9734017.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(30044..30943)
                     /gene="galU"
                     /locus_tag="VW325_000573"
     CDS             complement(30044..30943)
                     /gene="galU"
                     /locus_tag="VW325_000573"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9734018.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(30965..31981)
                     /locus_tag="VW325_000574"
     CDS             complement(30965..31981)
                     /locus_tag="VW325_000574"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9734019.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            32193..32632
                     /locus_tag="VW325_000575"
                     /pseudo
     CDS             32193..32632
                     /locus_tag="VW325_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(32747..33172)
                     /locus_tag="VW325_000576"
     CDS             complement(32747..33172)
                     /locus_tag="VW325_000576"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9734020.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(33240..33890)
                     /gene="phoU"
                     /locus_tag="VW325_000577"
     CDS             complement(33240..33890)
                     /gene="phoU"
                     /locus_tag="VW325_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9734021.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKTLLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLSDLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(33905..34657)
                     /gene="pstB"
                     /locus_tag="VW325_000578"
     CDS             complement(33905..34657)
                     /gene="pstB"
                     /locus_tag="VW325_000578"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9734022.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWDEVKDNLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(34659..35474)
                     /gene="pstA"
                     /locus_tag="VW325_000579"
     CDS             complement(34659..35474)
                     /gene="pstA"
                     /locus_tag="VW325_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9734023.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(35467..36330)
                     /gene="pstC"
                     /locus_tag="VW325_000580"
     CDS             complement(35467..36330)
                     /gene="pstC"
                     /locus_tag="VW325_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9734024.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(36448..37323)
                     /locus_tag="VW325_000581"
     CDS             complement(36448..37323)
                     /locus_tag="VW325_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU9734025.1"
                     /translation="MKFKKILTLAAIGLSGFGLVACGNQSATSKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(37490..38821)
                     /locus_tag="VW325_000582"
     CDS             complement(37490..38821)
                     /locus_tag="VW325_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9734026.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(38822..39529)
                     /locus_tag="VW325_000583"
     CDS             complement(38822..39529)
                     /locus_tag="VW325_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9734027.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGIEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGKQEGDDSDNIADNSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(39613..40098)
                     /locus_tag="VW325_000584"
     CDS             complement(39613..40098)
                     /locus_tag="VW325_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9734028.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAESPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            40084..40642
                     /locus_tag="VW325_000585"
                     /pseudo
     CDS             40084..40642
                     /locus_tag="VW325_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(40957..42648)
                     /locus_tag="VW325_000586"
     CDS             complement(40957..42648)
                     /locus_tag="VW325_000586"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9734029.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            42784..43230
                     /gene="argR"
                     /locus_tag="VW325_000587"
     CDS             42784..43230
                     /gene="argR"
                     /locus_tag="VW325_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9734030.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            43281..45815
                     /gene="mutS"
                     /locus_tag="VW325_000588"
     CDS             43281..45815
                     /gene="mutS"
                     /locus_tag="VW325_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9734031.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            45962..47656
                     /gene="patA"
                     /locus_tag="VW325_000589"
     CDS             45962..47656
                     /gene="patA"
                     /locus_tag="VW325_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9734032.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(47986..>48234)
                     /locus_tag="VW325_000590"
     CDS             complement(47986..>48234)
                     /locus_tag="VW325_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9734033.1"
                     /translation="RLIDKAKKCVNDLMAEFNSVITTVTGIENRLGAVILAEIRNIHA
                     FDNPAQLQAFAGLDSSIYQSGQIDLAGRMVKRGSPHLR"
BASE COUNT        14028 a         9097 c        10518 g        14591 t
ORIGIN      
        1 aaccatacct acggcaaggt gaagctgacg tggtttgaag agattttaga agagtattaa
       61 tgatagataa agtggtcagg aacttactcc tgaccttttt cttttgcaaa atgacaaaaa
      121 tcataaactt tttgacaaat atacttgtca aaaagaaaaa gatatgttac aatgaattca
      181 agttaagaaa taggaagcag aaaggagtta taatgtgggt actaggattt atactattta
      241 tgattttctt ttattctaat aattctaaaa aaatcaagaa actagagaat aaaatcaaaa
      301 aacttgagcg aaaagagaaa ggaaacgcag aaatgtcgag attattacaa gaaatgattg
      361 gaaaggaacc aattataacg ggagtgtata ttgggccaga taactgggaa gttgtggatg
      421 ttgatgagga atgggtcaaa ctacgtagtg ttgataatac gggaaaagaa aaattcaagt
      481 tgcaacgtat tgaagatatc caaaccgttg aatttgacgg agagtaggtg tgtaacgtgc
      541 aactaaaaaa tcgtctaaaa gagcttcgag ctcgcgatgg tctcaatcaa accgacctag
      601 ccaaactggc aggggtttcc agacagacca ttagcctact agaacgggat gagtacaccc
      661 catccattat cattgccctg aaaatatctc aaattttcaa cgaaacagtc gaatcggtat
      721 ttcgtttgga ggaggacgag tgatgaacaa gtataaagtg atttattatg tagtggtcat
      781 agctttatta gtcagcgtat ttctactgat tgggatggac ctaagctggt ttaatcccta
      841 tcaaagcgac caatttgttt gggtctactt tgctctcatc ccagtaattg aatggattga
      901 aaagaaatcc aaaaatctag caagtgaaaa aggagaatga atatgaaaaa gaaacgtaga
      961 ttgttatttt tgatgtctat tgtcttgggg gggttcttgg gcatgtttgt agggatgttt
     1021 aaggcacgtg tcgaatccca cgaaattatt ttagatgtaa aagccttgat gccatggata
     1081 tcagctattt gtttactgat aggtttcatt agtatgtttt tgactttcaa tttcttaaag
     1141 aaaagcagaa aatttcattc cttgtatcaa gaggaaatgg atgacgatct gaatgaaacc
     1201 tattatgtgc aaatgtatcg gaatcttgag tttggaacca ttgcttttaa tattacaggt
     1261 gtagcgattc cattggctat ttttatttca ttaagtgagg tgattatatt gcatacaaac
     1321 cctcaaacat ttttcctttc tttcttactc tttgtggtat tcttagtcgc tcaaaaatct
     1381 ctttttaaaa ccattgcgat tgttcgtcag tttgatttgg aatttttcgc tacaccaaag
     1441 gatgtcttga actatataaa ttcttatgat gaaggggagc gtcaggctaa tttggaacag
     1501 agttttcgaa ttttattcca attacaccaa tatgtcttac cagccttata tatttttctt
     1561 attatcattt ctttcttgac aggagagatt cagttactag ctttcttgct tgtaggagcc
     1621 atccatgttt atatcaatgt gatgcagtta cctatggtaa aacgttattt caaataaagg
     1681 agttatctat gaaattactt aaaaaccttg gctggattct tctagccctt ctatcctttt
     1741 tctttatcta tggctttatt caggggctcg cgactgcgtc gcttgcttta ggcgcttcac
     1801 cctatgctgt tactctactc tatgtggtct tggctggagt atatgtgtac ggcatttaca
     1861 aatggtatca gaaagctcct gtttatattg agaagagcgg ctttaaccga tttatttggc
     1921 ttcctgcctt ggtttggttc ttatctctgg tcgttcaatt cttcttgcca gatgatcctt
     1981 cagtaaatca gcaaatagcg acagacttga ccttgtctca accacttttc tcattctttg
     2041 ctgtggttat ttttgctcct ttgacggaag agattgtttt tagagggatg ttagcacgct
     2101 atctctttcc taagcaggac aatagtaaac gaaccctgat ttttcttctg gtatctagtc
     2161 ttctatttgc cttgattcat tttccaggtg atgtgcaaca attttttgtc tattttagcc
     2221 ttggttttag tttgggtttg gcttacgtta gcagaaaagg tctggtctac agtatttctc
     2281 ttcacgcttt gaataattta gtcggctttt tgatgattct catgctataa tagagtcagg
     2341 aggtcacatg aaacgagtaa ttttattagc agtgattcag gcagtcgttc tatttttcat
     2401 cattggagcg ctagcttatg ccttcaaagg cgatttcttc tataactatc tagcagttgt
     2461 ctttgctcct attgcaggtg tactgcgttt tgggacggct tacataacgg aaattgtctt
     2521 gcctcgaaag gcagccgaaa tcgctgaaaa gcgtaaagca ggcaaaaatt caaaataaaa
     2581 gagtccggtg aagttcatcg gatttttgta ttatactcaa tgaaaatcaa agagcaaact
     2641 aggaagctag ccgcaggttg ctcaaaacac tgttttgagg ttgtggatag aactgacgaa
     2701 gtcagctcaa aacatggttt tgaggttgta gatgaaactg acgaagtcag ctcaaaacat
     2761 ggttttgagg ttgtagatga aactgacgaa gtcagtaacc atacctatgg taaggcgacg
     2821 ctgacgtggt ttgaagagat tttcgaagag tattagcgct aatttttagg gctttcacct
     2881 gacttttaaa gactggcaaa cttttctgat gattttcatg aagagcctgc gcttttatgg
     2941 taaaatagta acagaataaa agaggagaga aataatgaaa cgtagtatgt atgctggtcg
     3001 tgttcgtgag gaacacatcg gacaagaaat aaccttgaaa ggatgggttg gccgtcgtcg
     3061 tgaccttggt ggtttgatct ttatcgatct tcgtgaccgt gaaggaatca tgcagttggt
     3121 tatcaaccct gaaaaagtat ctgcagaggt tatggcaaca gctgaaagcc ttcgtagcga
     3181 atttgttatt gaggtgactg gtcaggtcgc tgcgcgtgag caagccaatg ataagttgcc
     3241 aactggtgcg gttgagttaa acgtgacagc tcttattgtg cttaacacag ctaagacaac
     3301 accatttgag attaaggatg gcattgaggc aaatgacgat acacgtttgc gttaccgtta
     3361 ccttgacctt cgtcgtccag aaatgttgga aaatcttaaa cttcgtgcca aggtgaccca
     3421 ctctatccgc aactatttgg atgagttgga gtttatcgac gtggagacac cattcctttc
     3481 taagtcaacg cctgaagggg cgcgtgatta tttagtaccg tctcgtgtta ataaggggca
     3541 tttttacgct cttcctcaaa gtccacaaat cacgaaacag ctcttgatga atgctggttt
     3601 tgaccgttac taccaaatcg ttaaatgttt ccgtgacgag gacttgcgtg gagaccgcca
     3661 gcctgaattt actcaggtcg acttggaaac gtccttcctt actgagcaag aaatccaaga
     3721 tattacagaa ggcttgatcg cgcgcgtgat gaaggaaaca aaaggcatcg aagtaacgct
     3781 accgttccct cgtatgaaat acgatgatgc tatggctctt tacggttctg acaagccaga
     3841 tactcgtttt gacatgttgc ttcaggactt gacagaagtg gtcaaaggtg tagactttaa
     3901 agtcttttca gaagcacctg ctgtaaaagc gattgtggtc aaaggagctg cggacaacta
     3961 ttcacgtaaa gacatcgaca agatgacgga agtagccaaa cagtatggtg ccaaaggtct
     4021 tgcttgggtc aaggtggttg atggagaatt aaacggacca gttgccaagt tcttgactgg
     4081 tatccaagaa gaattgacaa cagcgcttgc tcttgaagat aaggacttgg ttctctttgt
     4141 ggcggatacg cttgaagtgg ctaatgcaac actgggtgcc cttcgtggac gtattgctaa
     4201 agagcttggc ttgattgata atgataagtt caacttcctt tgggtggttg actggccgat
     4261 gtttgaatgg tctgaagaag aaggccgcta catgagcgcc caccatcctt tcacccttcc
     4321 acaggaagag actgctcacg aattagaagg tgatttggct aaggttcgtg ccattgctta
     4381 cgatatcgtc ttgaacggtt atgagcttgg tggtggtagc cttcgtatca accaaaaaga
     4441 ccttcaagaa cgcatgttca aggctcttgg tttctcaact gaagaagcca atgaccagtt
     4501 tggtttcctt cttgaagcca tggactatgg tttcccacca cacggtggtt tggctatcgg
     4561 gcttgaccgt tttgtcatgt tgcttgctgg agaagaaaat atccgtgaag tcattgcctt
     4621 tcctaagaac aacaaggcaa ctgacccaat gacacaagct ccatcaacag tcgctctcaa
     4681 acaactagag gaactcagct tacaagtaga agaagatgaa acaaacaaaa cgaattaagc
     4741 ggtggcgcta ttatctgcgc cgctttgctt atcagataaa aattttacgt gtcttacaaa
     4801 gtatctctcg agaaaagtat gatgagaaga tttcggcctc tctggtctat ggttttttat
     4861 cagcagtagc agttaatttc tttttccaac cagggcatgt gtattcgagt ggtgcaacag
     4921 gtctggcaca gattatctct gccttgagta atcactggtt tggttttcat attccgattt
     4981 cgctaagctt ctacgccatt aacttccctt tgatggtctt agcttggtat cagattggcc
     5041 ataagttcac cgtctttacc tttatcacgg tatctatgag ttccttcttt atccagtttg
     5101 tccctgtggc aaccttgacg gaggatccca ttatcaattc cctttttggg ggtgttgtta
     5161 tgggtttggg gattggtttt gctcttcgaa acaatatctc cagtggtggg acggatatcg
     5221 tcagcctgac tattcgcaag aaaacgggta agaatgtcgg tagtatttct ttcttggtaa
     5281 atggaactat catgctgata gcaggtttaa cctttggttg gaaatacgct ctttattcta
     5341 tgattaccat ctttgtctct agccgtgtga cagacgcagt ctttactaag caaaagcgta
     5401 tgcaggccat gattgtgaca aatcatccag agaaggtaat tgaaaaaatc cataaaaaat
     5461 tgcaccgcgg agcaaccatg atccacgatg cagaaggaac ctataatcac gagagaaagg
     5521 cagttttaat cactgtcatt acacgtgcag agtttaatga atttaaacag attatgacac
     5581 aagtggatcc aagctccttt gtctctgtct cggaaaatgt tcatattcta ggaagatttg
     5641 ttgagataga taattagtga ttaatactct tcgaaaatct cttcaaacca cgtcagcttc
     5701 accttgccgt aggtatggtt actgacttcg tcagtttcat ctacaacctc aaaaccatgt
     5761 tttgagctga cttcgtcagt ttcatctaca acctcaaaac agtgttttga gcaacctgcg
     5821 gctagcttcc tagtttgcac tttgattttc attgagtata aaaagatctt gggaaaagac
     5881 tcaatttcag tagaaaagga agtaaatctt cccacattaa aacgcatagt atcaagtttt
     5941 ttcaatacct gatactatgc gtttttgctt ttaacgactt tttgcccagt ctcttatttt
     6001 tcgataattt tgtgggcgat caattgacgg taacaaattt gtttattgtt tttattatca
     6061 ttaataatct ggagaatagt ttcaagggaa acacgaccaa gctctaaact attgatatcg
     6121 acataggctg ccaagttgag cttgggatta accgagtcaa agctgagaac agggacatcc
     6181 agctggtgtt tggcaatata gttacaaaca ccttcagcta ggaggctatc ggttgtgatg
     6241 atagcatcaa tttgtggatc gtgcttgaat aatcgcttgc taaatttata gcccttttct
     6301 tccagaaact cgtcggcaaa gtagatgcga ttgttgtcag tggtaagttt gtaatgttta
     6361 agcgcctgtt catagcctgt taaacggtct ttggtcacga agagcttttt acttcctccg
     6421 ataaaggcaa tgcgtttgca gccttttttg atgaaatatt cagtcgcatc aaaaccagct
     6481 tgaacattgt cgttgtcgac aagtgggatg aaaggagata gagatttacc taagataagg
     6541 aaggggaact gttcttctgc gacgagtttt acgagagggt cttcttcttg ggcatagaga
     6601 aagattagcc catctacacg cttgccgtag accatttgtg aaatagcgtt gagacgctcc
     6661 ttctcatctt tccctgttgc tatctgaatg gcatagtggt tttcagatgc gacttgagag
     6721 atgccacgta gaaccgatgg aaagaaagga ttctggtaga aggcgtctga gtcatcagga
     6781 agaactaatc cgataacctg agtatagctg cttaccaagc tacgagcgtt gaggtttggg
     6841 tggtagttga gttccttcat agctttgcga acgcgttttt ttgtttcgtc gctaatggtt
     6901 gatttatttt gaataacacg ggttacggtt gaaggcgaaa caccagcagc cttggccacg
     6961 tctttaatcg taacgggcat aaaaatctcc tatttatggt aatttgaatc acggaaatgt
     7021 gttttataaa agatagtgac cagatacaga acaaaaagaa tattttgtac agtaatcagg
     7081 gttgtcatat tttggccaaa taatcccaaa ataagtgtaa tcagagtcgg caatcctaaa
     7141 cagttcaaga taaagtgata gcactcttta aaggttctaa atgaaaagag gcgtgatttc
     7201 ttagtgatat aaaggagaag actagctcct agagagacga taaagaaatt caaaccaaag
     7261 aggaagctcg caccgagaac taggaagagg ctgatataga cacgattttg ttggtaccag
     7321 tctttagaaa ttgcttgggt caagctgtct ttgcttttga aactctcagt ctgaatggct
     7381 cggtaagaga tgcgggtcag ttccttgctt tccttgctga tgactagctc atttgtatcg
     7441 aaatgcagtt gcaagtcctt aggtaattcc ttgatttgac ttggaccaat cacaacagaa
     7501 ggggcttgac tagctgttcc agtataagtt aatgtgccat cgacaattgt agcatgttca
     7561 gagagatcct ggacaacctc atttgtcaga ggttcataga tattatcgat aaaagtttct
     7621 agcggatagg tctcctggga gctgttttgg atagcaatgg gtaccataga taagctgata
     7681 aggaagatac tggtaaagag aagttgaaac cagttgagct cgaaacgttt ggacaggggc
     7741 ttacgaaacc cccaaatact tgaaaaatag gaaaatggat atggaagcat aggtatcttt
     7801 ctaaaaggtg ttttctatat gagtattatt atatagagaa atgcgcttta tttcaagtct
     7861 aggagaaaaa ttgcttgcta caaggatatt atttacagtg acaattgcta ataatcaatg
     7921 aaaatcaaag tgcaaactag ccacaggttg ctcaaagcac aactttgagg ttggagataa
     7981 agctgacgtg gtttgaagag attttcgaag agtataaaat gaaaaagggt ggcggggata
     8041 aattatccct tgtcgccacc acttgtaagt cctgaaacaa agttcttttg taggaagaag
     8101 aagagaatac agattggaag ggcgatgagg atagcacctg ctgagaagta ggcaatcttc
     8161 aagtttttcg cattgttaac gaaggtttgg agacctacgg caacagtaaa gtattctttc
     8221 tcacgaagca agaaactaga gaggatgtag tccccgaaag gtcccatgaa ggcccagaga
     8281 gcttgtacgg caaccattgg gcgaacaagt ggaagaacaa tttgccagaa gcggcggaag
     8341 tgtcctgcac cgtctagttt tgcagattcg tctaaagaca ttggcactgt atcgaagtag
     8401 cctttcatga gccaagcatt catcgggata ccaccaccaa cgtagaggaa gatgaggaac
     8461 cagttgtggt taagggcgtt caacataagc gccataacga agaaggctgt caaagcggcc
     8521 atagttggca ccatttggat gatcaagaag aagaccaaac tttgtttacg agccaagaag
     8581 ttgtaacggc tgtaggcata accagcaagt acgatgatac ttgtttgaac agccatggta
     8641 attaaggcga taatcaaagt gttgaggtac caagtaccgt acaaggtttc agtgaagagg
     8701 cctttaaagt tatcaaaatt gaggtcgata ttagtatcta gtttaaaggc tgagacgtta
     8761 cctgctttaa aggctgacat aatggtaatc aacagtggat agataattac aattgataga
     8821 ccaatcaggt aaaggtaagt aaggctttga gtcagtctac gtttgagttt aattgagtta
     8881 ttcatcttag acgtcctcca tatcaaatgc gtgtagtttc ttgaatgcga tcatagagat
     8941 tgagatgaca atgatagaga taatcaaggt aacagctgcc gccattgagt attgaggaga
     9001 tgtacctgtt gtcaaacggt agatccatga gatcaagata tcggttgaac cagctccacc
     9061 tccgacacta ccaggtcctc caccattgaa gaggtacatg atagagaagt tgttaaagtt
     9121 gaaggtgtat tggctaatca aagtaggtgc cgcaacagcc aaaatcattg ggaaagtgat
     9181 gttgcggaat ttttgccaag cgttggcacc gtcaatataa gctgcttcgt aaaggtcgtt
     9241 aggaatagat tgcaagatac ccaaagtcaa aacgtagatg tatgggaatc cgagccaacc
     9301 ttgcatcata atcaaggcaa tcttagtcca agttgggtct gttttccaag gaataagagc
     9361 tccatcaagg aaaggaagga atttagccaa gattggcaat acttgagtgt tgatagcacc
     9421 gacactatcg ttaaacatgt ttgagaatgt caagatagtg atgaaggctg ggacagccca
     9481 aggaagaagg aaaataacac caaagatacg ttttcctttg ataaatggtt ggttggcaat
     9541 gatagctgtg aagataccaa ttacgatttg taaagtagaa gctgccaaag cccaaatgat
     9601 agtccaagaa agaacagaac caaaggcaga acggaaggta ctcaagctcc aaatgtttgt
     9661 aaagttggtc aaaccaaccc agtccaacaa cttgtttggt ggcaagtgtt ggaagtcgta
     9721 gttggtaaag gcgatcatca aggttacgat aactgggaag ataatcgcga aggtcatggc
     9781 aacataagat ggaatgatca agaggtaagg gaagccattt tcataaatcc ctttgatcat
     9841 gtctttgagt gtgcgtggaa ctggaattcc attgttaatg cgttttgcaa tcgtatgtgc
     9901 atctttgata tttgagaaat aaaagagtac ataaacgatt acaaagatta gatggaaggc
     9961 accacgaatc agcataaaga gggaattatc acgacctggt ttgtcaccaa gagtgatgag
    10021 gttgctcaat tcaggggttg caagtgctag gaagtaaagg acaaatacga tggttacacc
    10081 aaggaagata aaacctttgg ctttttgttt attgtaaatc tgtcctaacc caggaatgat
    10141 agacagcagg gctgctttac taggttgttg cttttccata ttaactcctt tcataggata
    10201 ctactttttt ataagaaagc taaattattt gtgtctctat tgataaattc aaagggggat
    10261 ttgatttcca ccccccttga acaaattttt tattcaccaa atttttgttt gattgtttct
    10321 ttgatcaatg ttacagcatc gttagcagct gttttagcat ctttttgacc acttacagca
    10381 tcaaagagca tatttttcgc tggatcccaa actgcagaca tttgagagat gtttggcagt
    10441 ggttgagtgt tcttgaactg tttgataaca gctgttgtca actcatcgtt tttaccttca
    10501 gcgtatgaac gagcctcagt attagctggg atttcgttag tcttatcata taatactttt
    10561 tgttgttcag ttgcaacaag gaagtctaca aatttttgag aagcttcaag gttcttaacg
    10621 gcttgaggaa tgacccaagc tttaccacca ccgaatgcag catattcttt tccatttgga
    10681 agagttggga tagttgcaac tccgtagttt actttagcat ctttaaaggc ttgagctttc
    10741 caaggtccgt cgatgatagc agctgtttta ccttcttgga attgagtttg gattaagttt
    10801 ccagcacctt ctgtatcttg cataccttta ggccattttt cgtaccaaga tttagcgtag
    10861 ttgatacctg cgatagaacc gtcgtttgca agaccgatgt ctttagcgtc tttaccgttt
    10921 tggccaaaga cgtaagcacc gttaccggca agaagtccat atgtatagta gaagtttgtc
    10981 cagtcagcta ggaaggcagt agttttacca tcttcaccag cgaatgcgta tttgctatct
    11041 ttagcaaggt tttccaagtc agcaaatgtt tttggagcat ctttcaccaa gtctttgttg
    11101 tagtacataa caagtgactc gataacggca ggagcaccgt aaactttacc attagcagct
    11161 gttacaagag atttagttgt gtcgtctgtt ttagcaccat cgctcaattt cacttctgaa
    11221 agttgtccgt cagaaccaag gctacctaca cggtcgtatg gagccatcat aacatcaggg
    11281 acattaccag attggttgtc aagagaaagt ttatcaagac ctcctagagc atcaccagtt
    11341 ttaagagtga cttttactcc agcttctttt tcataagctt tagcaacctc ttcaatatag
    11401 ctcttatatc cctcgtctac atatacagtg agttctttga cttcagatga accagaatca
    11461 gcaggcttat cagcagtttt gcttccgcaa gctaccaaaa gcaagctagc aagtgtagca
    11521 gttccaagca ccgcagtgct cttcataaat ttagatgaca tagtgtattc ctcccaaaga
    11581 atagcaagtt ttattgataa ggaaacgcaa acgttttcct ttatgagctt agtatagcac
    11641 aaatagaaaa cggttgcaag tattttttgt aaaaatttta aaaaattttt aagcttgatt
    11701 tgatagcata attttgcatt ctaatagaag aaaagtatga aatagataag aatacagtat
    11761 ttttaataaa aatatatgga atctgtctaa agagggaaat caggaggggc tacctccctg
    11821 tttctaatcc ctatctattt tctatagacg tttgtgcttt gttgcatata aattcaagct
    11881 ccctaaggag gttttgtact ttgtagactg tctcgtgaga gtcaaacttt aggattagat
    11941 agttttaagt tatctttgct aaaatgagtt agtctcctta aaaataatca aaaaagtttt
    12001 ttaaaaacgc ttgcaattat gcttgaaaag gagtatactt ataagtaacg caaacgtttg
    12061 cgtctgcaaa atacgcaacg ttccattatt ttaacacacg aggtgctatt atgaaaaaac
    12121 gtcaaagtgg tgtgttgatg cacatctctt ctcttccagg agcttacgga atcggatcat
    12181 ttggtcaaag tgcttacgac ttcgttgatt tcttggtccg tacaaaacaa cgttactggc
    12241 aaatccttcc attaggagca actagttacg gggattctcc ttaccaatct ttctcagcct
    12301 tcgcaggaaa cactcatttt atcgatttag atatcttggt ggagcaaggt ttgttggaag
    12361 caagtgacct tgaaggagtt gactttggta gcgatgcgtc tgaagttgac tatgctaaaa
    12421 tctactatgc acgtcgtcct cttttagaaa aagcggtgaa acgtttcttt gaagtcggag
    12481 atgttaaaga ttttgagaaa tttgctcaag acaaccaatc atggcttgag ctctttgctg
    12541 agtatatggc tatcaaagag tattttgaca atcttgcttg gactgaatgg ccagatgcag
    12601 atgctcgtgc tcgtaaagct tcagcacttg aaagctatcg tgagcaattg gcagacaagt
    12661 tggtttacca ccgtgtgact caatacttct tcttccaaca atggttgaaa ttgaaagctt
    12721 acgctaacga caaccacatc gaaatcgttg gggacatgcc aatctacgta gcggaagatt
    12781 caagtgatat gtgggcaaat ccacatctct tcaaaacaga tgtcaatggt aaggctactt
    12841 gtatcgcagg atgcccacca gatgagtttt ctgtaactgg tcagctttgg ggtaacccaa
    12901 tctatgactg ggaagcaatg gacaaagacg gctacaaatg gtggattgaa cgcttgcgtg
    12961 aaagcttcaa aatctacgat atcgttcgta tcgaccactt ccgtggcttc gaatcttact
    13021 gggaaatccc tgctggttcc gatacagcag cacctggtga gtgggtgaaa ggtccaggct
    13081 acaagctttt tgcagccgtt aaggaagaac ttggtgagct aaacatcatc gcagaagacc
    13141 ttggcttcat gacagatgaa gtgatcgaat tgcgtgaacg tactggcttc ccaggaatga
    13201 agattcttca atttgccttc aacccagaag acgaaagcat tgatagccca cacttggcac
    13261 ctgctaactc agttatgtac acaggaacac acgataacaa tacggttctt ggttggtacc
    13321 gtaacgagat tgatgatgcg actcgtgagt acatggctcg ttacacgaac cgtaaagaat
    13381 atgaaacagt ggtacacgct atgcttcgta cagtattttc atcagttagc tttatggcaa
    13441 ttgcaactat gcaagattta ctagaattgg atgaggcagc tcgtatgaac ttcccatcta
    13501 cccttggtgg aaactggtct tggcgtatga ctgaagatca attgacacca gctgtcgagg
    13561 aaggtttgct tgacttgaca acaatttatc gccgaattaa tgaaaatttg gtagatttaa
    13621 agaaataaga caataatcag gagacaacta aacatgttat cactacaaga atttgtacaa
    13681 aatcgttaca ataaaaccat tgcagaatgt agcaatgaag agctttacct tgctcttctt
    13741 aactacagca agcttgcaag cagccaaaaa ccagtcaaca ctggtaagaa aaaagtttac
    13801 tacatctcag ctgagttctt gattggtaaa ctcttgtcaa acaacttgat taaccttggt
    13861 ctttacgacg atgttaaaaa agaacttgca gctgcaggta aagacttaat cgaagttgaa
    13921 gaagttgaat tggaaccatc tcttggtaat ggtggtttgg gacgtttggc tgcctgcttt
    13981 atcgactcaa ttgctactct tggtttgaat ggtgacggtg ttggtcttaa ctaccacttt
    14041 ggtcttttcc aacaagttct taaaaacaac caacaagaaa caattccaaa tgcatggttg
    14101 acagagcaaa actggttggt tcgctcaagc cgtagctacc aagtaccatt tgcagacttt
    14161 actttgacat caactcttta cgatattgat gttactggtt atgaaacagc gactaaaaac
    14221 cgcttgcgtt tgtttgactt ggattcagtt gattcttcta ttattaaaga tggtatcaac
    14281 tttgacaaga cagatatcgc tcgcaactta actctcttcc tttacccaga tgatagtgac
    14341 cgtcaaggtg aattgctccg tatcttccaa caatacttca tggtttcaaa cggtgcgcaa
    14401 ttgatcatcg acgaagcaat cgaaaaagga agcaacttgc atgaccttgc tgactacgca
    14461 gttgtccaaa tcaacgatac tcacccatca atggtgattc ctgaattgat tcgtcttttg
    14521 actgcacgtg gtatcgagct tgacgaagca atctcaattg ttcgtagcat gactgcctac
    14581 actaaccaca caatccttgc tgaggcgctt gaaaaatggc ctcttgaatt cttgcaagaa
    14641 gtggttcctc acttggtacc aatcatcgaa gaattggacc gtcgtgtgaa ggcagagtac
    14701 aaagatccag ctgttcaaat catcgatgag agcggacgtg ttcacatggc tcacatggat
    14761 atccactacg gatacagtgt taacggggtt gcagcactcc atactgaaat cttgaaaaat
    14821 tctgagttga aagccttcta cgacctttac ccagaaaagt tcaacaacaa aacaaacggt
    14881 atcactttcc gtcgttggct tatgcatgct aacccaagat tgtctcacta cttggatgag
    14941 attcttggag atggttggca ccatgaagca gatgagcttg aaaaactttt gtcttatgaa
    15001 gacaaagcag ctgtcaaaga aaaattggaa agcatcaagg ctcacaacaa acgtaaattg
    15061 gctcgtcact tgaaagaaca ccaaggtgtg gaaatcaatt caaattctat ctttgatatc
    15121 caaatcaaac gtcttcacga gtacaaacgc caacaaatga acgctttgta cgtaatccac
    15181 aaataccttg acatcaaagc tggtaacatc cctgctcgtc caatcacaat cttctttggt
    15241 ggtaaagcag ctccagccta cacaatcgct caagatatca ttcacttgat cctttgcatg
    15301 tcagaagtta ttgctaacga tccagcagta gctccacact tgcaagtagt tatggttgaa
    15361 aactacaacg ttactgcagc aagcttcctt atcccagcat gtgatatctc agaacaaatc
    15421 tcacttgctt ctaaagaagc ttcaggtact ggtaacatga aattcatgtt gaacggagct
    15481 ttgacacttg gtactatgga cggtgctaac gtggaaatcg ctgagttggt tggagaagaa
    15541 aacatctaca tcttcggtga agattcagaa actgttatcg acctttacgc aaaagcagct
    15601 tacaaatcaa gcgaattcta cgctcgtgaa gctatcaaac cattggttga cttcatcgtt
    15661 agtgatgcag ttcttgcagc tggaaacaaa gagcgcttgg aacgtcttta caatgaattg
    15721 atcaacaaag actggttcat gactcttctt gatttggaag actacatcaa agtcaaagag
    15781 caaatgcttg ctgactacga agaccgtgac gcatggttgg ataaagtcat cgttaacatt
    15841 tctaaagcag gattcttctc atctgaccgt acaatcgctc agtataacga agacatctgg
    15901 cacttgaact aatactcttc gaaaatctct tcaaaccacg tcagctttat ctgcaacctc
    15961 aaagcggtgc tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga
    16021 gtataagata caaatttata ctaatacatc ttgtaaaaaa gcgagtttcg attgaaattc
    16081 gcttttttaa atgctgtgga tttgggtcaa tcttgtctaa aaatagggaa atcctagata
    16141 cagtgaaggc tttaaatgct ggtttttact gtcttcagcc ttatattttt tcgtagttgg
    16201 ttacctcaca tctcttgtat tcgcttacat aaagtattat aatataattg taggaaagaa
    16261 ggtgttttta tgatatacac acttaaattg gtgttgttta ttacctttct tgtaataagc
    16321 ttgttacctg ataagatttt tggaaaaaat aaaaaaattt ggaaaatagt ttttgcaata
    16381 ttgacggcag tggcagcatt gtcatttatg tactaagtta ttttaagaat gtagggaaat
    16441 aaaccctaca ttttttttag ttttttctgt tttctaaatt ctatttatcc aagcgattca
    16501 acatttcttg cttcttcgct tcaagttctg cacgcttttc ttcgatttcg gcatgttttt
    16561 tctcgagttc agaacaactt gcaccattgc taaattcttt tcgccatcag gagatagggt
    16621 gagtcgacat gtctattact cacccaaagc agtcctacaa agcaggaatt ttctgttact
    16681 tttttggaaa tagtaacgtt tatacagctt tgacacttcg tatcaaagcg ccaaacacac
    16741 tccgagggat ttacagaaag cagaaaagga atgatctggt ataagatcat tccttttctc
    16801 tctttttctt taagtaatta tatacaatgt acgacgaagt cgtcattgca atgctgatcc
    16861 accacctaaa gggaacttta aacaacattg ataagataaa gaatataaac aacgaaaata
    16921 cgttataccc aattaatttt attgtatatc tcatgattaa aagttaatcc ttccgttgtt
    16981 aggaatggca tcatttttat cccataattg tgctaaataa gtccctggtg ataataaatt
    17041 catagcgaat tctaaagcaa catcatttac aaaccaacta cctagatatc tagaaattgc
    17101 tgaacgaata gcactttttg ctgcatgttt tccttttact ttaattagat ttgcaaggcc
    17161 tgcagtagtt ccttctaatg ctaaagctat tgcagtatct aatagagcac ccatttgatt
    17221 aactgtaata ccttgccaag ctgctctaaa tggagagtat gtaggtggga ttgtataatc
    17281 gccttgtaat tgtcggttaa ttacttcttt gatccattgt tgtgagacgt ctggatgaaa
    17341 agattggatt tcgtttgcaa gtgtattgat ttgttcttct gttagagaag tgacaggttg
    17401 aagttccata tttgtttcaa tttgcgatac ttgttcagaa gcgtatacag ctgaaacact
    17461 tggaatcgct gatacaatta acacaattga cgtcaaaaaa accgaaataa atttcattaa
    17521 tttgttcatg agcttttctc ctttttattt gtatctgctt acattttatt atatactgtt
    17581 attatagtca agaaaaatat gctattatgt taaaaaaata tttttcaaaa tataaatgga
    17641 cggatttatt ttggatttta tttgttattt tgacctgcct ctatattggt aaccatgatt
    17701 tgtttactct caatcatcaa gaattctctt ttcgtggtag cgtttggggt ctggtactgg
    17761 ccttatatca cttattgttc attgataagt ttgtaatttc gaatcgaaaa taaagattag
    17821 aactatgatt gactggctac ttttaatatt gattttggag gaagattttg tctctattat
    17881 ttattatttt aaatttatct attttgtaca agatctattc tttgagacgg gagaaatcga
    17941 aatacttgat ttatacggcc tatatcatat ttggggtaaa tgtagtatat ggtattcaat
    18001 ggttattaag agaactgatt tcaactattt ccccttaatg tgtatggaaa ctataacaaa
    18061 acgcataaga ttaaggtttg tgtacctgtt cttatgcgtt tttgtaatat tgaagatttt
    18121 cggtaagttt tatacctttg ggaagtctag tttatactca atgaaaatca aagagtaaac
    18181 tagccgtagg ttgctcaaag tacagctttg aggttgcaga taaaactgac gaagtcagct
    18241 caaaacatgg ttttgaggtt gtagatgaaa ctgacgaagt cagctcaaaa catggttttg
    18301 aggttgtaga tgaaactgac gaagtcagta accatacgta cggcaaggcg acgttgacgt
    18361 ggtttgaaga gtattagaat gctttcccaa ccaaaatctc tgcttcgatg gtaatctctg
    18421 tcagtttgct ccagtcaatt ttgctctggt caacgtgaaa gaggagagga gtcatactga
    18481 gtagggcttg gagttgttct gctgtgatag tcttagtcag agaggcagtt tgactagata
    18541 ggatggtaaa gtgttcctgg aaatgctcct tgatatcttg gttagaatac tccttgtttg
    18601 tcagctggtc ctgtactctt tgacggattt ctttgaggtg attttcagtt gggataacct
    18661 ttatcaagat accgtctttg gataaaacgc gacgaaattc tccatagttg gcaggtgaga
    18721 agatatcaag cagaatatcc atgttagcgt cttttatagg aagtcgtgcc aagtcgccaa
    18781 cgaaccaatt gactgcccag ttgggttcac ttttagcagc gatttggact gaatctttgg
    18841 agatgtcaaa ggcatagaaa gttttttcag agtgactttc ttgtagtttg cgagaataga
    18901 atccttcacc acaaccgata tccaaaattg ttgtggtagt ttttgagctt gcaagcaagt
    18961 cagatacagc atctaagata gcttggtaaa agccggcttc taggatttgt tgacggtttt
    19021 gaaaattttc cttgtcatag ttagcagatt gcttgatttg aggtgctaga ttgacatagc
    19081 caaatttcgc caagtcaaaa gaatgacggt tgcagcactt gaaattagtc tctaacagag
    19141 tcagattttc ttgacagata ggacaggcaa aggcagtcgc agaagcaaaa cgctgaagtt
    19201 tgggcttgag atttgtattc atagtttaag tgtatcaaaa gaggcaaatg aaagcaactt
    19261 taggaataag tagatgggag attcagtaga aataaaacta attctccaat ttatagaatg
    19321 aaattgcttt tatatctaaa ttgctatata ataatgataa ggaggaaata agtatgttaa
    19381 aagaagtatt aaccgttgca aaagttgcga aaaaatcttc attatttttg ggtggtgtcg
    19441 catttggtac ccttggtttg aaaatcttag caagtaagga agctaaaaaa ggttattcta
    19501 aagctttggc taaggcttac aagttgaaag acgagctaga tgcatctgtt tctgtcgtga
    19561 agcaacatgg agacgatgtc ttgcaagatg ccaaatattt gtacgagcaa gagaaaaaag
    19621 aagagcaatt agatagcctt ataggtgaat aatatgtctt ttaaagtgct acatagagga
    19681 taccaacata tccgactatc atcttctttt tcacttacct tggatattca agactatctt
    19741 cgttccttgg cgagagatga aaaggggatt gagtctatcc agttttacat ggatcaacag
    19801 cactttactc tacgcataaa agaaggcttt tctgtattag ataatgcaga agccttttta
    19861 aaaagaattg ataaagggaa agtttctgag ttgatgactc ttcccattcg tagagaagag
    19921 agtgcttatt ctattgtttc aggtgcagcg gttaagcgtg tactttttcg tagttttgtg
    19981 ccgtatccta ttcgctatat atggacttgt tatcaggctt tgggttatat tagagaagcc
    20041 tatcaaacac tagcgcgtaa ggaactaacg atggaagtct tggactgttc ggctatttta
    20101 ttgtctttgt ttatgaacca atccaagaca gctagcaata tcatgtttat gcttgatttg
    20161 gggaatcatt tagatcagtg gtccttgaaa aaaactgcaa cagatttaga acagagtctt
    20221 cttgcaaaag agagcgatgt attcctagta cagggcgata cagttgttag tatcaagagt
    20281 tccgatgttc aaataggaga tgtcttgatc ttatctcaag gaaatgaaat tctgtttgat
    20341 ggacaagtag tttcaggttt aggtatggtc aacgaaagtt ccttgacagg agagagtttt
    20401 ccagttgaaa aaagagagtc tgatttggtt tgtgcaaata cagtattaga aactggagag
    20461 ttacgcattt gtgtaacaga taatcagatg aacagccgta ttttacagct gattgagttg
    20521 atgaagaaat ctgaagaaaa caagaaaacg aaacaacgct atttcatcaa gatggcggat
    20581 aaagtcgtca aatataattt cttgggggct gggctgactt acctattgac aggttctttt
    20641 tctaaggcta tttctttcct attggtcgat ttctcctgcg ctttgaaaat ctctactcct
    20701 gtagcttatt tgacagttat caaggaaggg ttgaaccgtg aaatggtgat taaggatgga
    20761 gatgttctgg agaaatatct ggaagttgat actttcttgt ttgataagac aggaacaatc
    20821 acaactagtt atcctatagt tgaaaaggtg ttaccttttg gagactatag tgaggaagat
    20881 attctcagaa tcagtgcctg tcttgaggaa cacatttatc atcctattgc taatgccatc
    20941 gtcaagcaag ctgagataga gggaattgaa catgaggaaa tgcatgggaa actccaatat
    21001 atcgcaagca aggggatcaa atctcatata gatggccaac cagttcttat tgggaattat
    21061 gtcttgatgc aggatgagca gattcatatc agttcagaac aaaatgcttt aattgaagag
    21121 tacaagagtc actacaatct cttattcttg gcttatcaaa atgaattgat tggaatgttc
    21181 tgcattcata ctcctttgag aaaagaagca aaaacagcct tggataaact taaggcacag
    21241 gggaaaaaat tgattctggc aacaggagac accttgatta ggacagagga attagtcaaa
    21301 gatttgccct ttgatcaggt ctatacagac ttgaaacctg atgggaaatt tgagttagta
    21361 gagaaactgc agaaagcagg tcacactatt ttgatggttg gagatggatt gaatgactca
    21421 gcggctttaa ctctatcaga tatcggtgtg gtgatgaatg agagtgcaga tatttctaag
    21481 cagatgagtg atatcttatt gttagataat cgtttggatt tcttccaaga gttggattcg
    21541 ttatcatcat ctttgcaaac actcatcaag aaaaatattc aggataccgt tgtcgtaaat
    21601 agtagtttga ttggctttgg cttatttaat tggttcagtc cttcaaatct ctctatctta
    21661 cataatctaa caaccttgcg cattgtcctg cgtagcctgt ctattaaaaa tagatagatg
    21721 agagttatta acagcaaaaa ggagttacct ttctcgaggt taactccttt gtttatagat
    21781 aaatgttgaa tagtttagta agtcaataca aagtattttt tcttcccacg acggataaca
    21841 gtcagttcat tttctaactt atcagcgtca ctcaagacat agtcaaggtc ttggatgcgg
    21901 tcgccgttta cgtagatggc tccgttttgg acgtcttcac gggcttggcg ttttgagtta
    21961 actataccag atgagacgag cagttccacg atattgttgt tttcgtctgc ctgtacttgg
    22021 tagtttggca caccacgaag tccttgtttg agctctttga cagaaaggtt tttgatgttt
    22081 cctgcaaaga gttgctcagt gatgttaagt gcttctttgt aggcttcttc tccgtgaaca
    22141 agtgtaacaa cttcacgagc caagactttt tgagccaagc gttcgtgtgg cgctgcttca
    22201 aattgtttac gaatatcttc aatctcatca agtgacaaga aagtaaagat tttcaagaag
    22261 cgaacagcgt cagcgtccat cacgttcatc cagaattggt acatttcgta tggagaagtc
    22321 ttttcgggat tgagccagac ggcatttcct tctgatttac caaatttttt accagttgca
    22381 tctgtgatta gtggaacagt gataacgtga ccagtcttgt ccgccttacg acgaagcaat
    22441 tcggtaccag ctgtcatatt tccccactgg tcagaaccac cgatttgaag agtgacatta
    22501 tggtcttggt taaggacgaa gaagtcgtac ccttgcatga tttggtaagc gaactcagtg
    22561 taagaaattc ctgtttcgat ccgtttttta acagattcct tactcatcat gtagttgacc
    22621 gtgaagtatt ttccaatatc acggaggaag tcaatgaagc tgatgctgcc aaaccagtcg
    22681 tagttgttga ccatgacagc cttgttttcg ccattttcaa agtcaagaaa acgagaaagt
    22741 tgtccttgga tagacttgac ccagccatct actgtgtctt ttgtttggag actacgttca
    22801 gcatctttga aggacggatc tccgatgaga cctgtagcac cgccaacgag cgcataaggt
    22861 ttgtgacctg ctagttgcaa gcgacgactt gtcaagattg cgacaaggtg gcctaggtga
    22921 aggctgtcag cagttggatc gtagccagta taataagaaa cttgaccttc ttctagggct
    22981 ttacgcaaag cttcttcatc agtcgtttga aatatcaaac cacgctcttt tagctcatca
    23041 aaaatgtgca tgtgtctttt ctccttttta aaattattgt ttctacctat tgtatcacaa
    23101 acttgggcaa tagcctagca gaatagggaa gaaagtggct attttattga aaatttccct
    23161 tttgtggtat aatagataaa gtgaggaaat ccatgcaaaa tcaattaaat gaattaaaac
    23221 gaaaaatgct ggaatttttc cagcaaaaac aaaaaaataa aaaatcagct agacctggca
    23281 agaaaggttc aagtaccaaa aaatctaaaa ccttagataa gtcagccatt ttcccagcta
    23341 ttttactgag tataaaagcc ttatttaact tactctttgt actcggtttt ctaggaggaa
    23401 tgttgggagt tgggattgct ttggggtacg gagtggcctt atttgacaag gttcgggtgc
    23461 ctcagacaga agaattggtg aatcaggtca aggacatctc ttctatttca gagattacct
    23521 attcggacgg gacggtgatt gcttccatag agagtgattt gttgcgcact tctatctcat
    23581 ctgagcaaat ttcggaaaat ctgaagaagg ctatcattgc gacagaagat gaacacttta
    23641 aagaacataa gggtgtagta cccaaggcgg tgattcgtgc gaccttgggg aaatttgtag
    23701 gtttgggttc ctctagtggg ggttcaacct tgacccagca actaattaaa cagcaggtgg
    23761 ttggggatgc gccgaccttg gctcgtaagg cggcagagat tgtggatgct cttgccttgg
    23821 aacgcgccat gaataaagat gagattttaa cgacctatct caatgtggct ccctttggcc
    23881 gaaataataa gggacagaat attgcagggg ctcggcaagc agctgaggga attttcggtg
    23941 tagatgccag tcagttgact gttcctcaag cagcattttt agcaggactt ccacagagtc
    24001 ccattactta ctctccttat gaaaatactg gggagttgaa gagtgatgaa gacctagaaa
    24061 ttggcttaag acgggctaag gcagttcttt acagtatgta tcgtacaggt gcattaagca
    24121 aagacgagta ttctcagtac aaggattatg accttaaaca ggacttttta ccatcgggca
    24181 cggttacagg aatttcacga gactatttat actttacaac tttggcagaa gctcaagaac
    24241 gtatgtatga ctatctagct cagagagaca atgtctccgc taaggagttg aaaaatgagg
    24301 caactcagaa gttttatcga gatttggcag ccaaggaaat tgaaaatggt ggttataaga
    24361 ttactactac catagatcag aaaattcatt ctgccatgca aagtgcggtt gctgattatg
    24421 gctatctttt agacgatgga acaggtcgtg tagaagtagg gaatgtcttg atggacaacc
    24481 aaacaggtgc tattctaggc tttgtaggtg gtcgtaatta tcaagaaaat caaaataatc
    24541 atgcttttga taccaaacgt tcgccagctt ctactaccaa gcccttgctg gcctacggta
    24601 ttgctattga ccagggcttg atgggaagtg aaacgattct atctaactat ccaacaaact
    24661 ttgctaatgg caatccgatt atgtatgcta atagcaaggg aacaggaatg atgaccttgg
    24721 gagaagctct gaactattca tggaatatcc ctgcttactg gacctatcgt atgctccgtg
    24781 aaaatggtgt tgatgtcaag ggttatatgg aaaagatggg ttacgagatt cctgagtacg
    24841 gtattgagag cttgccaatg ggtggtggta ttgaagtcac agttgcccag cataccaatg
    24901 gctatcagac cttagctaat aatggagttt atcatcagaa gcatgtgatt tcaaagattg
    24961 aagcagcaga tggtagagtg gtgtatgagt atcaggataa accggttcaa gtctattcaa
    25021 aagctactgc gacgattatg cagggattgc tacgagaagt tctatcttct cgtgtgacaa
    25081 caaccttcaa gtctaacctg acttctttaa atcctactct ggctaatgca gattggattg
    25141 ggaagactgg tacaaccaac caagacgaaa atatgtggct catgctttcg acacctagat
    25201 taaccctagg tggttggatt gggcatgatg ataatcattc attgtcacgt agagcaggtt
    25261 attctaataa ctctaattac atggctcatc tggtaaatgc gattcagcaa gcttccccaa
    25321 gcatttgggg gaacgagcgc tttgctttag atcctagtgt agtgaaatcg gaagtcttga
    25381 aatcaacagg tcaaaaacca gggaaggttt ctgttgaagg aaaagaagta gaggtcacag
    25441 gttcgactgt taccagctat tgggctaata aggcaggagc gccagcgaca agttatcgct
    25501 ttgctattgg tggaagtgat gcggattatc agaatgcttg gtctagtatt gtggggagtc
    25561 taccaactcc atccagctcc agcagttcaa gtagtagttc tagcgatagc agtaactcaa
    25621 gtactacacg accttcttct tcaagggcga gacgataatt ctctaaatga agtggccaat
    25681 caatggattg ccactttttt cttttcgtgt tacaataagg gtatgaatca gtatcagaaa
    25741 aagattgtta acggaaaaat ttattcgctc ctatccggct taatatgggg aatctgtgga
    25801 attttaggag agtacttctt tactcattat caggtgtctt cgggctggat tacctctatg
    25861 cgtttgacac tggcagggag tcttgtactc atttggtctg caatacaatt aaaatcgcaa
    25921 gtgctagata tttggcgaga caagaaaaat tacctgccct ttttagccta tgctattttg
    25981 gggatttttt cagttcagta ttttttctat ctctgtgtag aatactcaaa tgctacgaca
    26041 gcaactattt tacagtttat tagccctgtc tttatcctct tttacaatcg cttggtttat
    26101 caaaaacgag cgtcaaaaag cgctgttttc tatgttttgg ttgccatgct gggtgtttgc
    26161 ttgatggcaa caaagggaga tctctctcag ttatccatga cgccgctagc tcttataaca
    26221 ggtttgctga gtgccatggg tgttatgttt aatgttatct tgccccaacc ttttgctaag
    26281 cgttatggtt ttgttcctac ggttgggtgg gggatgattt tggcaggttt gtttagcaat
    26341 gtcctctcgc cggtttatca gctttccttt actcttgata tttggagtat cttgatttgc
    26401 ctcattatcg ctttctttgg aacggctttt gcttttttca tttccatgaa ggctgtgtcc
    26461 ttggtttctc ctttggtggt tgccgttatc agtgccagtg aacctctctc ttctgctctc
    26521 ttgagtgttt tgttcttagg attagtagtg gattggtccc tccttctagc tatagccttg
    26581 attattttac ccatgatatt tttgtctata gaagaagcga aagaaagtag ataaaaagtc
    26641 ttttcttaat tctaaaagtg tgctatacta gaatagtcaa taaaacacgg ggagatagtt
    26701 gtgaagagtg tttttaggtt gtatcggtag gggcttgctt ttaccgacag cacgttttat
    26761 ctcacatccc taaaaaatca tacctaaaaa cagacaaaaa ggtttcaaat ttgaggcctt
    26821 ttttggtaga ataggtatca ttataacgaa ccaggaggca cctatgactg ctacaaaaat
    26881 gaacgctcaa gaaattatcc aatttatcgc caatgctgaa aagaaaacca gtgtcaaagt
    26941 aacctttgag gggcaactcg caactgctgt gcctagctct gttgtcaaac taggaaatgt
    27001 cctattcgga gactggaagg acgtggctcc gcttcttgaa ggtttggtag aaaatcaaga
    27061 ttatgttgtc gagcaagatg ctcgtaattc tgcagttcct ttgctagata agcgtgctat
    27121 caacgctcgt atcgagccag gtgctattat ccgtgaccag gtggaaattg gtgacaatgc
    27181 tgttatcatg atgggagctg ttatcaatat cggtgctgaa atcggtgctg gaaccatgat
    27241 tgacatgggt gccatccttg gtggccgtgc catcgttgga aaaaatagcc acgttggtgc
    27301 aggtgcagtt ttggcaggtg tgattgagcc agctagtgct gaaccagtcc gtgtcggaga
    27361 caatgttctt atcggtgcta atgcagtggt tatcgaagga gtccaaatcg gtagtggttc
    27421 agttgtcgct gcaggagcta tcgttaccca agatgtccca gaaaacgtgg tagtagcagg
    27481 tgttccagct cgtatcatca aagaaattga tgcccaaacc caacaaaaaa cagcgctaga
    27541 ggatgcgctt cgtactttgt aattgtaaaa gtaaaaaaga ggcggaaccc tttttccagc
    27601 ctctttctgc tatatcggag gacagataga tgttagattt gattcagact agacgagctt
    27661 tacaccagat tccagagatt ggcttggagg agttcaagac tcaggcttat ttgctggatg
    27721 tgattgagaa attgactacg ggcaaggatt ttgttcaaat tcgtacttgg cggacaggga
    27781 ttttggtcta cctgcaggga agtcagccgg agcgaaccat tggttggcgg acagatattg
    27841 atggcctgcc tatcgtcgaa caaacaggtc taccttttgc ttctcaacac caaggtcgca
    27901 tgcatgcctg tggccatgat tttcatatga ccattgcctt aggctgtctt gagcgcgccc
    27961 ttgaggagca acccaagaat aatctgctct ttctatttca gcctgctgaa gaaaatgaag
    28021 ctggtggtat gctcatgtat gaggacggtg cttttggaga ttggttgcca aaccaattct
    28081 atggtctcca tgttcgtcca gatttgaaag tcggccagat tgcgactaat actcatacac
    28141 tctttgcagg aacttgcgag gtgaagatcc gtttcaaagg caaaggtggc cacgcagctt
    28201 ttcctcatga agccaatgac gccttggtgg cggctagtta ctttgtgacc caagtgcagt
    28261 cagttgttag ccgcaatgtc aacccaatcg agggagcagt ggtgaccttc ggcgtttttc
    28321 aagctggaac caccaacaat gtcatcacag acacagcttt tttacatgga actattcgtg
    28381 ccttgacaca ggacatgagt ctcttggtgc aaaagagggt aaaaacagtt gcagaagggg
    28441 ttgcagctgc ctttgatatg gaagtcgaag tggaactcaa acaaggtgga tacctacctg
    28501 ttgagaacaa tccagccttg gcgcgtgaac tgatggactt ctttgacgaa aaagacggaa
    28561 tcgagttgat tgatatcgaa cctgctatga caggcgagga ctttggttat ctcctttcaa
    28621 aggttgatgg cgtgatgttt tggctaggta tcgatagtcc ctacgctctt catcaccctc
    28681 aaatgagtcc taaggaagaa gtcttagcta ttggggtggc tgcggtgtct agtttcctga
    28741 aaaagaaggc agcagaatag agggcttgtc tatgaaatcg gaattacgca agcaagtctt
    28801 gcatgaaatg aaggctttat ctcaagaaca aaaacaggct atagaccaag ctttaaccga
    28861 gcgaatttta caacacccct tttatcaaga agccaaggtc atcgcaacct acctctcttt
    28921 ttctcatgag tttcaaacgc gggaactgat tgagcaggcg ctgaaggacg gcaagaaggt
    28981 tttaataccc aaaacttatc ccaaggggcg catggacttt gtagtctatg atccgcagca
    29041 gttggtaaaa actgcctttg ccttactgga gccacaggga gatttggaag tggtggatgt
    29101 atctcagatt gatttgattc atgttcctgg cctggctttt acgacggatg gatatcggat
    29161 tggatatggt ggaggttatt atgaccgcta tctggaacat ttttctggtc atactttgag
    29221 tacggttcat ccttgtcaaa ttcaggactt tatacctgaa aaccatgata ttcctgttca
    29281 ggaggtatta attgatgaag gaaatctttg atagacgtta ccctgtgacg agtttcttcc
    29341 tcttagtgac ggccttggta tttttactaa tgttggtcac tgcaggcgga aactttgaca
    29401 gggcagatac attatttcga tttggagcca tgtatgggcc agctattcgc ctctttcccg
    29461 agcaggtttg gcgtctcttg tctgccattt ttgttcatat tgggtgggaa catttcattg
    29521 ttaatatgct ttcactttat tatcttggaa ggcaggtaga ggagattttc ggttctaagc
    29581 agtttttctt tctctatctt ttatcaggaa tgatgggcaa tctctttgtt tttgtattta
    29641 gtcctaaatc cttagcagca ggcgcctcta cctctcttta tgggctattt gccgcgatta
    29701 ttgttcttcg ctatgcaact cgcaatcctt atattcaaca gctagggcaa tcctatctga
    29761 cactttttgt ggttaacatt attggaagtg ttctgattcc aggaatcagc ctagcaggcc
    29821 atatcggtgg tgcagttggt ggcgcatttc tagcagttat ctttccagtt agaggagaaa
    29881 aacggatgta taataccagc cagagattag gagcggtagt cttgttcgta ggactcgcca
    29941 ttttgctttt ctacaaggga atgggaatgt gacgaagttg aaagaaaatt tgtgtaatga
    30001 aaaaagataa ggcatttttt gaaccttatc tttttatttt attttattcc ttctcagtca
    30061 attcttttcc aagttggatg aggtaattct tcaaatcatc tttgacttgt gggtgtttga
    30121 gggcgtagtc gatggatgtt ttcatgaagc caaacttgtc tccgacatcg taacgagccc
    30181 cattgaactc acgagcaaat acacgttgtg ttttattgag ggtgtcgatt gcatctgtca
    30241 gctgaatttc atttcctgca cctggagctt ggttttcgag gatttggaaa atttcaggcg
    30301 tgaggaggta gcgtccgata atagcaaggt cgctaggagc gtcctctgga gctggttttt
    30361 caacaaaggt ttcaacactg taaagaccat cttttccttc gccttgcgga gcaataaccc
    30421 cgtaagcaga tacttcgtca tgagggactg gcatgacagc gatagtagac gcgtgggtac
    30481 gctcgtagtc atccatgagt tgtttggtaa gtggaacagc cttttcgtct gtgatatcca
    30541 tcaagtcatc accaagcata acgacaaaag gttcatttcc gacgaaagcc ttggcttgca
    30601 aaacagcatc tccgagaccg cgtggatgag tttggcggat aaaatgcagc cgcatgtcag
    30661 ttgttttatc aactagcttc aaaagatctg ttttcccttt ttctttgagg ttatattcca
    30721 attcgaagtt tgaatcaaag tggtcctcaa tagaacgttt tgatttacca gtgacaacta
    30781 gaatatcttc aatacctgat ttgagagctt cttccacgat aaactggata gttggtttgt
    30841 ctacgattgg caacatttct ttggcaaggg ccttggttgc tggtaaaaat cgagttccta
    30901 gtccagcagc agggatgact gcctttctaa cttttgatgt cataaaaatc ctttctatag
    30961 agggttaaga ccactcattt tctgctttaa attcattgtt catgatgtca taaatggcat
    31021 ctttgatatt ggttccgtga taaataactt ggtaaatagc ctgtgtaatg ggcatataga
    31081 ctccaagttc ttgggctagt tcataggctg ctcgagtcgt tgaaattcct tcgattacca
    31141 tgcccatatt agcttctata tcagctaggg attctcctcg tccgagagca tctccagctc
    31201 tccagttacg agagtggatg gaagttcccg ttacgatcaa atctcccaca ccagataagc
    31261 cgctataggt caatggactg gccccgagtg ctacccctag gcgggtgatt tctgctaaac
    31321 ctcgagcgat gatggctgcc ttagcattat caccaaatcc aagaccatgt aaagctccag
    31381 caccgacagc aataatattt ttaagagcac cagcagtttc aaccccgata acatccgtat
    31441 tggtataaag tcggaagtag tgattactaa atagcttctg aacgtattga gctgtttgta
    31501 aatctttaga agcagcagtt attaaagtta ggtcacgcac aatggtctct tctgcatgac
    31561 taggccctga aacaacgacg atatcactac ggagatgttc aggaatttct tcttcaagaa
    31621 tggttgataa tcgtttatgg ctatcaggtt ctaatccctt tgatgcgtgc atgatgataa
    31681 ccttatggtc caaggtttgt gcaacttgct gggcaacaag tcgtgtcact tttgttggga
    31741 caacaaacaa aatcgcatcc acatctttca atgtttctgc taagtcggtg taggcaatga
    31801 tattttcgtc tagaacgaca tctttaaagt agtgcttatt agtatggtgt gtattaattt
    31861 cattgatttg ctcgggaaga tttccccaaa tacgtacctc gtgtccattg tcatttaaga
    31921 cttgtgaaag ggcggttccc caagaaccag gccccaagac ggcgacggtt tgtttttcca
    31981 tgatttcctc cttgatagag ttctttctct tattctatca tattctaggg gattttgcac
    32041 ttgcattact ggggtatggt ggaaaaatac acaaaaaccg agacgaaggg ataaactctt
    32101 agtctttagt taaagtgaga agaaatctga tagatagttt gattcagtat atgcttataa
    32161 tagaggtaaa caactcagga ggttcttgta ggttgcgaga gtttggcgaa aaaattaaaa
    32221 gattacgttt ggctaaaaaa atcagtcgtt cagaattttg tggtgatgag tctgaattaa
    32281 gtatccgtca attaattaga attgaaaatg gagaatccag accaatacta acaaagttaa
    32341 aatatattgc tgaacgtttg gaggttgaag attacaagtt gatgccaagt tatatagagt
    32401 tggataagga atacctagaa ttgaagtatt tcttgatgag gactcctaca tacgaagatg
    32461 aaactatcgc ccaaaagaaa gagagtgttt tgataagatt tttgaagagt attatgatag
    32521 gctacctgag gaagaaagat ttatcatccc aaattattca tatctggcac taacgaacta
    32581 cacagttcaa aaattaccag aaaagctagt tgaaatactg tccttctggt gattttgctt
    32641 aatttctgat gttcgagaca taattgagga gatttgtctt gattttttcc taaaaaaggg
    32701 cagactcctc ccttggtttg atgctcgatt tttaaaataa ggtgacttat tctgttaggc
    32761 atgttttccc ctgccctcta gctctatatg gaacagggag tatcagattt atctggcaga
    32821 ttgtatcaaa taaggcttga aactgttttg aataggcata tagacttgag aatttgagaa
    32881 tatgtcgtct agcagtagag acatattttc cggcaatatg aaggaagaga cgtcgaaaac
    32941 gcttgatagt caaggccttt acttcatcac cagctagctg ctttaaaaag aggtagagat
    33001 tataagccag acagcccatc atcatacgta cttcattctt aatcatggtc gaactatctg
    33061 tcttatcccc aaagaatcct gctttccttt ctttgataaa gttttcagca ttccctcgtt
    33121 cacgatagaa ttgaaacagc tcctcactat ctacactgct taaattggtc aaatgaactt
    33181 ttttacaacc ataaaaaaga gcacttaatc gtactcttct cttttaagga ttagttgaat
    33241 taattcaaat ccactagttc tcctgtttct aggtagacta ggcgttcaca aatgttagca
    33301 atgtaatcag cgaagcgttc cagatgccct atgatataaa ggtattgtgt tccattggga
    33361 attgaggttt cttggtcttt cataagtcca atgatttcct ttgataaggc ataataatat
    33421 tggtcaatct gttcatcttt ttgagcaata ctaatagctt ttgaggcttg gtgcaaagga
    33481 aaggcaacca ataaatcgga tagcatgctg agggataatt tacccatttg gtgtaactgt
    33541 tcttcgtcag gggctagttg attttctttt agttgcaaaa cagctttagc aatgcctgcc
    33601 atatggtctc ccatacgttc aaggtctgaa caagaagaca tgatgctaat cacaaatcga
    33661 aggtcagaca cttgtggctg ctgcaaggcc aacaaacggg cacaggtcaa ttcgatagcg
    33721 ctttgacctt ggttgatagc atgatcctta ttgataatta gctctgccat ctccttgtct
    33781 ttggaggcta aggccagtaa ggtttttgaa gctgtttcaa ggacaagttg ccctagtcct
    33841 aaaaaggatt gttctaattc atgcaattct aagtcaaatt gatttctcat aggtttttcc
    33901 ttccttatcc gaaccgtcct gaaatatagt cttctgtgcg ctgatctttt ggattggtaa
    33961 acacgtcaac ggtgtctcca aattcgcaaa tttctcctgt taagaaaaaa gcagttttat
    34021 ctgaaatacg tgaagcttgt tgcatgttat gggtaacaat gataatcgta taatcctttt
    34081 ttagttgctg aatgaggtct tcaattttta aagtggagat agggtctaag gctgaagtcg
    34141 gctcatccat taacagaaca tcaggttcta ctgctaaagc tcgcgcaatg caaaggcgtt
    34201 gctgctgacc gcctgataag gacatggcac tctttttaag attatctttg acttcgtccc
    34261 aaatggctgc cccttttaaa gatttttcca ctaaggcatc taattgtttt ttgtctcgaa
    34321 taccatgtgt ccttggacca taagccacgt tatcatagat agacatggca aagggattag
    34381 gctgttgaaa aaccatccct acacgcttgc gtagctgatt aaggttgaat ttgctactat
    34441 aaatatcttg ctcatctaag aggacttggc cttcaatatg gcaagaagga accaaatcgt
    34501 tcatccgatt aagggttttt agaaaagttg atttgccaca accagatggg cctatcaagg
    34561 cagtaatctg tctttctggt aattgaatcg aaatattttt taaggcttga aaatccccgt
    34621 aaaataagtc taggtgtctg actgaaaatg ttcccatact aggaagctcc tttcacaagt
    34681 ttccgagata ataagcttga tagagtattt atcattaaaa cagtaataat taaaatcacg
    34741 ccggtagcat aggcttcatt gacatgtagc ccctcacttg acagcatata catatgtagg
    34801 gctagagaac ggcctgaaga catgagacta cttggcgtat tggtagaggt acctaatgta
    34861 tacatgaggg cagctgtttc accaacgata cggccaatag ctagtatcac tccagctaaa
    34921 atacctggca tggcaactgg tagaacaatt ctaaaaacag tccgtaactt acctgcccca
    34981 agtccataac ttgcttgacg catgctatca ctaacagata aaagggcttc ttctgttgag
    35041 cgaataatga ctggcaacac catgataact gaggttaaga ttcctgataa cagagagtat
    35101 tgaaaaccta agaagactac aaagaagagc atgccaaaca gaccaaaaac aatggaagga
    35161 atcccagaca aggtatctga ggccaatcgc atgattttaa cacaaaggga atcttttttt
    35221 gtatattcca caagataaaa accagcaaaa atccctatgg gcaaagctaa aagaagagca
    35281 ccaaaaacca gaataacggt ggaaataatc gctggcataa gggaaatgtt ctcagaagta
    35341 taagtccaag aaaagaggga tagacttaga tgaggtaagc ctttgatgag gataaaacca
    35401 atgattaaaa agagagagcc aaaggttaaa gctgaaaaac aataaacgag aagttttagc
    35461 aggtatttac tcataagatg attttccttt caagtaggca aagtaggcat taatcaagag
    35521 aataaggaaa aagagaactg ctgaggttgc aataagggct tccctatgct gacctgatgc
    35581 gtaagccatt tccagaacaa tattggttgt taaggttctg gttcctgaaa agagtccact
    35641 tggaataatt ggctggttgc ctgccaccaa aataactgcc atggtttcac ctactgcgcg
    35701 accgattcct aaaataactg ctgataaaat accagatctc gcagctggca agatgacact
    35761 aaaaatactc cgttcatgac tagctcctag agccaagcta ccagaataat acgttttggg
    35821 aactgttcgg atagcagatt ctgacaaact gataatggtt ggcaaaatca ttattcctaa
    35881 tagtaacgaa gcggttagga cactcatgcc atttcctaaa aaggttctaa tccaaggcac
    35941 caataattgt aggccgaaaa aaccataaac aatagatgga atggctgcca tcaagttgat
    36001 agctgatttt aagaagccat agacgggctt tggacaataa taaaccataa acaccgatgt
    36061 caagatgcct gttggcaccc caatcacaat cgctcctaag gtaattaata aggaaccaac
    36121 gatcattggt aaaataccat agcttgccgg aatgttcgtt ggcgaccaat cactgcctaa
    36181 taaaaaacgg gcaaagccgt agttagccat gaaaggtaag ccattactaa aaataaagaa
    36241 acagattagc aaaatagcta caacagctac tgttgcactc atgaaaaaaa ttgccctaaa
    36301 aactgcttct ttgaaggctt gttttgtcac atcttgtcct ttctagtgaa gaaagtaagg
    36361 gagatacgac acctccctac ttgccttctt tatcttattg tacgatgaaa cgtctgcatc
    36421 tctttagaga tttatggagc aaacatttta tttaatcttg tcccaggtgg ttaatttgcc
    36481 actaaaaacg tctgcaagtt cagccatact gacttggctt gccttattgt cattattgac
    36541 cacaacagca ataccgtcta aagcaatagc atcatgggtg agactcttac cttcttcagg
    36601 agttaattcc ctagaaacca taccaatatc agcggttttc tccttaacag cggtaatacc
    36661 tgctgaagac ccattagagg taatatcaat cgtaacttct ggattttctt ttttataagc
    36721 ttctgctaat ttttccatta aagaagatac tgaagtggaa cctacaacag acaacttgcc
    36781 tgataagtgt tggcttgtat attctgtggt ttcagtttta gcttcaataa atttattatc
    36841 tgtgaccact tgttgacctt gtttggagtg gataaagctg ataaaatctt gacctagctt
    36901 ggaaagatta gaagaccaaa caatgttgaa gggacgttga agagggtatt caccatctaa
    36961 aactgtgtct cgactagcct tgacaccatc aatctctaaa gccttgacag atttcgttaa
    37021 agatcccaag gagatgtagc cgatagcatt agcattccct tgaactgctg agagaacacc
    37081 ttctgtacta ttttgaatca cagctgtttt ggcagtgttg tcaatttttt tatcaccgtc
    37141 ttttttgaga atccctgtga tttctgtgaa ggcaccccgt gtcccagagc cattttctcg
    37201 tgaaatcacc tcaatcgttc ctgaagctga ctgtttggaa gtagctgact gattgccaca
    37261 ggcaacaagc ccaaatcctg ataagccaat ggctgcaaga gtaagtattt ttttgaattt
    37321 cataataatc acctttatct ctatgtattt ttcttgtgta ggcttactac atttatagtc
    37381 taacaagtct ttgtaaaggt ttatccctga ttcatgtaaa gattgtgtaa agaatcaaaa
    37441 aaagccactt ttgaaaaatg gctgctccta aaaatagctt taaaaattat tagtcctgtg
    37501 cgaaagattg gttaggaaga aaaatcgtga agcaactgcc tctgccaagc tgactcgtca
    37561 ccgtgacttg gccacctaat aattgactga gttctttgac aatggcaagg ccaagaccag
    37621 tgccaccagt ttgtctgctt cgacctttat taactcggta aaaacgttca aaaatacgat
    37681 cctgctctaa ttgactaata ccaatccctg tatctgatac agaaatctta atgccttcgt
    37741 tcaccttttg ggtcttgacc tcaatttttc ccccttgttc agtgtaacgg atggcattgg
    37801 ataaaagatt gagtaagatt tgggaaagta attgactatc tgatacgagg gtgacatcat
    37861 ctggcacctg cacctttagc tgtaaatcct tcttcttgag ctgaggttgc aagctttgag
    37921 tcaaatcctg tacaaattct gccaaagaaa gggtcgtcca ttgtataggc atttgttgag
    37981 ccttagataa ggtaagaaga tgctcaacaa tatgctcaag acgcaagctt tctttgtaaa
    38041 taatgtctag aaagtcatcc ttgagcgctt cttcttcagc tgacatcccc ttaatggttt
    38101 cagcaaagcc cttaatcgaa gtaactggtg tcctcaattc atgggaggca tttgagacaa
    38161 aggctaaatt taacttttca taagttctaa tcgttgttaa atcatatagc aagacgagca
    38221 cagcttccac agattgggtg gggctaaaaa cgggaactgc tgtcacttct aaaatcaagt
    38281 caccctcatg aaacccactt acttcttgtt ttaaccttgt tttttgatca aaggcttggt
    38341 gaactaaatt ccgaatatcc atccgtttga ggtcatcaag tgaacttatg tcgccgtcca
    38401 catcgggaaa ataatgaggc agagagcgac tggataataa catctgacct tgagcggaaa
    38461 ctaaaaacgt ccccatggtt aggtgcgaca gaagaacctc cattgtttcg gctaggtcct
    38521 tgtattgctg atcctgttgg gagactttgg tttttaggcc agacacatac tgagccaaag
    38581 actttaagtc ttcttgccct ttttctaaaa agtattcact actggtcaag agaggttggt
    38641 gcaaggtctc aaaagcaact tcccatttcc aaaggcaaaa gagccagtag ccacctagtc
    38701 ccaaagaaag ggctagaaga aagagaccga tgcctttact gatccaagtt aatgccatcc
    38761 ctgcaatcag aatgaggcta acacttagat tgactagcca aaattgaagg tagcgtttca
    38821 tctataactc cttgaactta taaccataac cccgaatggt tcgaataaat tgaggggctt
    38881 taggattgtc ttcaattttt tccctcaact taccaatatg aacgtccacc aaacgtgttt
    38941 cctgcccaaa gtcatacccc cagatacgtt ccaaaagacg ctctctagtc agtgtcatgt
    39001 tgggatgttt cataagatag agcaagagtt caaattcttt tggggtcaaa ctcagtaact
    39061 tattcgcctt gtagacttca tgacgctcag ggtatacttt caaggtccca aatagccaag
    39121 aattgtcagc gatattatct gaatcatctc cttcttgttt tcctttagtt cgcctgagga
    39181 cagccttgac acgcgccagt aattctctag ggctaaaagg cttggtcagg tagtcatcag
    39241 cccctaattc caaggccaaa accttatcaa attcatcact tttcgcagaa accatcataa
    39301 ttggagtttt gacgcctttg gctctcagcc gcttacaaac ttctatgcca tctaattgtg
    39361 gtaacatgat atcaagcaag ataaaatcaa agggttctgt ttctgccaaa gctaaggcct
    39421 tccgtccatt tgttaccaat tgagtagaaa agccttcctt acttaaatgg tagtcaagca
    39481 atttcagaat gtgttcttca tcatccacta ataagacttg ttttgtcatc tattatctcc
    39541 tattggtaac attataacac aattatcaga aatcctaaca ttgctaaatc agattaaatt
    39601 tgcttatcaa gactagtatc tggtcaaacg ctcaatcatc tccttgtgct ctggataggt
    39661 cgccagtaga tctacccttt caaataattc aaaatcctca aattcaaaac caggagcaac
    39721 aagacaagaa accagagcat catccttatc aactgttgat ccccaaatag tgcccttagg
    39781 aacacagtag tgaagttgtt gccctttgga tatgtccagg cctaaagtga ctgcttcgta
    39841 gtgaccatct gctgtaatca tgtgaacagt aagtggggat tctgcatgaa aataccagat
    39901 ttcatctgct gtcaatcggt gaaaatgtga aggattcgtt tcttctaata agaaataaat
    39961 actggtataa agcgcccttc ccttaccagc aaggtttata gtgtctgaag ctttttttgt
    40021 ttgtctaaaa tagccacctt caacatgggg agctaactct agagttctta tcaagtcttc
    40081 tttatccgtc ggagccaatg ggttgaagta actcttgttc aaagtggttt tacgatttca
    40141 agaactcctc tcagttctga ggacacggta atgattgatg cgacggaagt acaaatcaat
    40201 cgccctaaaa gaagaattag cgaatgattc tggtaaaaag aaatgccacg ctatgaaggc
    40261 tcaagcgatt gtcacaagtc aagggagaat tgtttctttg gatatcgctg tgaactattg
    40321 tcatgatatg aagttgttca aaatgagtcg cagaaatatc ggacaagctg gtaaaatctt
    40381 ggctgacagt ggttatcaag ggctcatgaa gatatatcct caagcacaaa ctccacgtaa
    40441 atccagcaaa ctcaagccac taacagttga agatcaagcc tataaccatg cgctatccaa
    40501 ggagagaagc aaggtcgaga acatctttgc caaagtaaaa acgtttaaaa tgttttcaac
    40561 aacctatcga aatcatcgta aacgcttcgg attacgaatg aatttgattg ctggtattat
    40621 caatcatgaa ctaggattct agttttgcag gaagtctatt atttggttag gtgaattagt
    40681 gaagcgttta ggcaagtgtc tctggttacg acgtcatggc ctctaaatcg attatattta
    40741 ggggtcatga ctagtgaagc agttagctag ttcgcatata agcggctagc gtctaacaat
    40801 taggaacttt agttccaata actttaagat tacgacgttt taggacataa atcgatcata
    40861 tttatgtcct aaaactagtg aagcgcctag ccaaagtccg aataggattt ggcgttagtt
    40921 acttagattg ctttgcaatc aagtaacttt ggcgatttac atcttctctg gcgcttctac
    40981 tccaagcaag cgaagggctt cttttagaac gactgcggtt gcgtagctga gggctagacg
    41041 gctgtcgcgt tctgggcttt catccaagat acgtgtatgt gcatagtatt tgttaaagga
    41101 ttgagctagg ctaattgcaa atttagcaat gatagaaggt tcaaagttat ctgccgcacg
    41161 gttgataata cgtgggaagt cttgaatgag tttaatgatt tcccagcttt cagtatcatt
    41221 caagctatag ttgccagctg tttctggttt gaaatcggct ttgcgtaaga tagattggat
    41281 acgagcgtag gcatattgaa cgtaaggtcc agtttcaccc tcgaaggata ccatagcttc
    41341 taggtcgaag tcgtatccat ttgtacggtc ggttttgagg tcatagaatt taatggctcc
    41401 aaccccaaca gcatgtgcta cttggtcttt gttttctagt tcaggatttt tagcctcgat
    41461 ttggaccttg gcacggctaa cagcctctgc aacagtaggc tctagcaaga tgacattccc
    41521 tttacgagta gagagcttct tcccttcttt tgtaaccaga ccaaaaggaa cgtgggtaat
    41581 gtcgtcactc caatcgtagc ccatctcttg caagacagct ttgagctgtt taaagtgggc
    41641 agattgttct tgaccaacga catagataga tttagcaaat tggtattcgt ttttacggta
    41701 gagggctgca gccaagtcac gtgtgatata gagagttgca ccatcagact tcttgatgag
    41761 ggctggatgt tcaattccat atttctcaag attcacaact tgggcacctt ctgattcaag
    41821 aagtagtcct ttttcagaaa gaatgtctac aactgcatcc atcttatcat tgtagaaggc
    41881 ttctccgtta tagctgtcaa attcaacctt caattcattg taaaggcggt taaattccac
    41941 taaactttca tcgcggaacc attgccaaag agcgagagct tcctcatctc cattttcaag
    42001 tttacggaac cattcgcgcg cttcttcatc caagctaggg tcattttcag cttcagcgtt
    42061 gatgcggaca tagagtttaa ggagttcatc gattggatga gcttttacag cttcttcgtc
    42121 gccccatttt ttgtaggcaa caatcaacat cccaaattgt ttaccccagt ctcccaaatg
    42181 gttgaccttg accgtttgat aaccgatttt ttggaaaata tgtgacaagc tatctccgat
    42241 aacagttgaa cgcaggtggc caatagaaaa tggtttagcg atattcggac tagacatgtc
    42301 gataacaaca ttttcttgtt tgccaatatt ttggtcagca tagtgttctt tttcagtggt
    42361 aacagcttgc aatacttgag cagaaatggc agatttatca aggaaaaagt taacgtaagg
    42421 tcctgttgcg acaacttttt caaaggcttg gctgttcatt ttttcagcca gttcagccgc
    42481 aatcatttgt ggtgctttac gttcgacttt tgcaagagaa aaagcaggga aagcaatgtc
    42541 tcccatttct gagtttttag gggtttccag taactttaaa atagcctctt ggtccaggct
    42601 atcaatgatg ctagataatt cgctagcaat caattctttt gtattcatta agagctcctt
    42661 tttggacttt tctactattt tatcacaatt ttaaagaaag aagaaaaaat ttttgaaatc
    42721 tcctgttttt ttggtataat atggttataa atatagttat aaatatgcac gcaagaggat
    42781 tttatgagaa aaagagatcg tcatcagtta ataaaaaaaa tgattactga ggagaaatta
    42841 agtacacaaa aagaaattca agatcggttg gaggcgcaca atgtttgtgt gacgcagaca
    42901 accttgtctc gtgatttgcg cgaaatcggc ttgaccaagg tcaagaaaaa tgatatggtg
    42961 tattatgtac tagtaaatga gacagaaaag attgatttgg tggaattttt gtctcatcat
    43021 ttagaaggtg ttgcaagagc agagtttacc ttggtgcttc ataccaaatt gggagaagcc
    43081 tctgttttgg caaatattgt agatgtaaac aaggatgaat ggattttagg aacagttgct
    43141 ggtgccaata ccttattggt tatttgtcga gatcagcacg ttgccaaact catggaagat
    43201 cgtttgctag atttgatgaa agataagtaa ggtcttggga gttgctctca agacttattt
    43261 ttgaaaagga gagacagaaa atggcgatag aaaagttatc acccggcatg caacagtatg
    43321 tggatattaa aaagcaatat ccagatgctt ttttgctctt tcggatgggt gatttttatg
    43381 aattatttta tgaggatgcg gtcaatgctg cgcagattct ggaaatttcc ttaacgagtc
    43441 gcaacaagaa tgccgacaat ccgatcccta tggcgggtgt tccctatcat tctgcccaac
    43501 agtatatcga tgtcttgatt gagcagggtt ataaggtggc tatcgcagag cagatggaag
    43561 atcctaaaca agcagttggg gttgttaaac gagaggttgt tcaggtcatt acgccaggga
    43621 cagtggtcga tagcagtaag ccggacagtc agaataattt tttggtttcc atagaccgcg
    43681 aaggcaatca atttggccta gcttatatgg atttggtgac gggtgacttt tatgtgacag
    43741 gtcttttgga tttcacgctg gtttgtgggg aaatccgtaa cctcaaggct cgagaagtgg
    43801 tgttgggtta tgacttgtct gaggaagaag aacaaatcct cagccgccag atgaatctgg
    43861 tactctctta tgaaaaagaa agctttgaag accttcattt attggatttg cgattggcaa
    43921 cggtggagca aacggcatct agtaagctgc tccagtatgt tcatcggact cagatgaggg
    43981 aattgaacca cctcaaacct gttatccgct acgaaattaa ggatttcttg cagatggatt
    44041 atgcgaccaa ggctagtctg gatttggttg agaatgctcg ctcaggtaag aaacaaggca
    44101 gtcttttctg gcttttggat gaaaccaaaa cggctatggg gatgcgtctc ttgcgttctt
    44161 ggattcatcg ccccttgatt gataaggaac gaatcgtcca acgtcaagaa gtagtgcagg
    44221 tctttctcga ccatttcttt gagcgtagtg acttgacaga cagtctcaag ggtgtttatg
    44281 acattgagcg cttggctagt cgtgtttctt ttggcaaaac caatccaaag gatctcttgc
    44341 agttggcgac taccttgtct agtgtgccac ggattcgtgc gattttagaa gggatggagc
    44401 aacctactct agcctatctc atcgcacaac tggatgcaat ccctgagttg gagagtttga
    44461 ttagcgcagc gattgctcct gaagctcctc atgtgattac agatggggga attatccgga
    44521 ctggatttga tgagacttta gacaagtatc gttgcgttct cagagaaggg actagctgga
    44581 ttgctgagat tgaggctaag gagcgagaaa actctggtat cagcacgctc aagattgact
    44641 acaataaaaa ggatggctac tattttcatg tgaccaattc gcaactagga aatgtgccag
    44701 cccacttttt ccgcaaggcg acgctgaaaa actcagaacg ctttggaacc gaagaattag
    44761 cccgtatcga gggagatatg cttgaggcgc gtgagaagtc agccaacctc gaatacgaaa
    44821 tatttatgcg cattcgtgaa gaggtcggca agtacatcca gcgtttacaa gctctagccc
    44881 aaggaattgc gacggttgat gtcttacaga gtctggcggt tgtggctgaa acccagcatt
    44941 tgattcgacc tgagtttggt gacgattcac aaattgatat ccggaaaggg cgccatgctg
    45001 tcgttgaaaa ggttatgggg gctcagacct atattccaaa tacgattcag atggcagaag
    45061 ataccagtat tcaactggtt acagggccaa acatgagtgg gaagtctacc tatatgcgtc
    45121 agttagccat gacggcggtt atggcccagc tgggttccta tgttcctgct gaaagcgccc
    45181 atttaccgat ttttgatgcg atttttaccc gtatcggagc agcagatgac ttggtttcgg
    45241 gtcagtcaac ctttatggtg gagatgatgg aggccaataa tgccatttcg catgcgacca
    45301 agaactctct cattctcttt gatgaattgg gacgtggaac tgcaacttat gacgggatgg
    45361 ctcttgctca gtccatcatc gaatatatcc atgagcacat cggagctaag accctctttg
    45421 cgacccacta ccatgagttg actagtctgg agtctagttt acaacacttg gtcaatgtcc
    45481 acgtggcaac tttggagcag gatgggcagg tcaccttcct tcacaagatt gaaccgggac
    45541 cagctgataa atcctacggt atccatgttg ccaagattgc tggcttgcca gcagaccttt
    45601 tagcaagggc ggataagatt ttgactcagc tagagaatca aggaacagag agtcctcctc
    45661 ccatgagaca aactagtgct gtcactgaac agatttcact ctttgatagg gcagaagagc
    45721 atcctatcct agcagaatta gctaaactgg atgtgtataa tatgacacct atgcaggtta
    45781 tgaatgtctt agtagagtta aaacagaaac tataaaacca agactcacta gttaatctag
    45841 ctgtatcaag gagacttctt tgacaattct ccactttttt gctagaataa catcacacaa
    45901 acagaatgaa aaggagctga cgcattgtcg ctcccttttg tctatttttt aaggagaaag
    45961 tatgctgatt cagaaaataa aaacctacaa gtggcaggcc ttggcttcgc tcctgatgac
    46021 aggcttgatg gttgctagtt cacttctgca accgcgttat ctgcaggaag tattagacgc
    46081 cctccttact gggaaatatg aagctattta tagtatcggg gcttggttga ttggtgtggc
    46141 cgtagtcggt ctagttgctg gtggactcaa tgttgtcctc gcagcctata ttgcccaagg
    46201 agtttcatcc gaccttcggg aggatgcctt ccgtaaaatt caaacctttt cttatgctga
    46261 tattgaacaa tttaatgcgg gaaatctagt cgttcgaatg acaaatgata tcaaccagat
    46321 tcagaacgtt gtcatgatga ccttccagat tcttttcaga cttcccctct tgttcatcgg
    46381 ttcgtttatc ctagcggttc aaaccttacc ttctctgtgg tgggtgattg ttctcatggt
    46441 agtcttgatt tttggtttga ctgctgtcat gatgggaatg atggggcctc gttttgccaa
    46501 gtttcaaacc cttcttgagc gcatcaatgc cattgccaag gaaaatttac gtggcgttcg
    46561 tgtggtcaag tcctttgtcc aagaaaaaga gcaatttgct aagtttacag aggtctcaga
    46621 cgagcttctt ggtcaaaacc tttacattgg ttatgccttt tcagtagtgg aaccctttat
    46681 gatgttggtt ggttacgggg cggtcttcct ctctatttgg ctggtcgcgg gaatggttca
    46741 gtcggatccg tctgttgttg gttccatcgc ttcttttgtt aattacctaa gccagattat
    46801 ctttaccatt gttatggttg gatttttggg aaattctgtc agccgtgcca tgatttccat
    46861 gcgtcgtatt cgagaaattc ttgacgcaga gccagctatg accttcaagg atatcccaga
    46921 tgaagagttg gttggaagcc ttagctttga aaatgtgacc tttacctatc caatggacaa
    46981 ggaaccgatg ctgaaagatg tgagctttac tattgaacct ggtcaaatgg ttggtgtagt
    47041 tggagcgact ggtgcaggaa agtcaacctt ggctcaattg attccacgtc tctttgatcc
    47101 acaggaaggg gctatcaaaa tcggaggtaa ggatattcga gaagtgagtg aaggaaccct
    47161 gcgtaaaaca gtttccatcg ttctccaacg tgccattctt tttagtggaa cgattgcaga
    47221 taacttgaga caggggaagg gaaatgctac tctatttgaa atggagcgcg cagccaatat
    47281 tgcccaggct agtgaattca ttcatcgtat ggagaaaacc tttgaaagtc cagttgaaga
    47341 acggggaacc aatttctctg gtggacaaaa acaaaggatg tcgattgcgc gtgggattgt
    47401 cagcaatcca cgtattctga tttttgatga ttcgacctca gccttggatg ccaaatcaga
    47461 gcgcttggtg caagaagctt tgaataagga cttgaagggg acgacaacca ttattattgc
    47521 tcaaaaaatt agctcggttg tccatgcaga caagatcttg gttctaaatc aaggacgatt
    47581 gattggtcaa ggcacgcatg cagacttggt tgccaacaat gccgtttacc gtgaaatcta
    47641 tgaaacacag aaatgaaaga caaactataa gaaaagtcaa tagttttatc taaactattt
    47701 cttatttcaa tttgatgatt tggcgatgat tttagagcac ggcaaaaagc tattgaaaaa
    47761 gtccattttt tcaaaggtaa tcctgtgtta atttcagaaa ttacatcact ttttgttcgt
    47821 caaatggcag ctctttttta ggatataaaa cagggttcgg ataagttttt ttgcaaggtg
    47881 gatgatggct acattgtaat gttttccttg ttctaactta gtcttaagat aggccttaaa
    47941 agcaggtgaa aagcgagggc atgctttggc agcttgtatg agtacctacc gcagatgagg
    48001 ggaaccccgt ttgaccattc ttcctgctaa atcaatctga cctgactgat aaatagaaga
    48061 atccagtcca gcgaaagctt gtaattgagc aggattatca aaggcatgaa tatttcgaat
    48121 ctcggctaaa atgaccgccc ctaaacgatt ctcaatccca gtaaccgtcg tgatgaccga
    48181 gttgaactca gccatcaagt cattgacaca ttttttcgcc ttgtcaatga gcct
//
LOCUS       DAVKLX010000009        45351 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKLX010000009 DAVKLX010000000
VERSION     DAVKLX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45351
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.009"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            75..851
                     /locus_tag="VW325_000591"
     CDS             75..851
                     /locus_tag="VW325_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734034.1"
                     /translation="MRTNILKLSQFLNIFYLKIALLIFSCLYFIAPWFIVLKDFETYK
                     VQYPAVIGIAEFIVLTSFLVFVYISPIFFVIFLYKIIVGSINVRRCLIKIKNYKFYMI
                     VLNRIPLHTFMVYFSSVLLWIMYLTKPVDVSNALPLPIILPLEKINPFYMILGYWLLN
                     KRCEYQLHQYLYPVMRGSSHDGRIYALPEELFIWNRNRSQKIGKWKRDRRYNTTTDLV
                     EAQIGYSKFPFFLLATKWSVDPYDQNWTIGEKAYLTSRWK"
     gene            1027..2415
                     /locus_tag="VW325_000592"
     CDS             1027..2415
                     /locus_tag="VW325_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9734035.1"
                     /translation="MKSKTVLVTSLAAVTFGLVSLVDTAGALPFSAKTVQADTQTLTT
                     AAKYYILVPKDSEVFKNWRGTANRPIIWATKTTPELSYFQESARNYIANENKSGANYI
                     EWKGTVEEFKEAIKNLTDKKLTPNPAPIPQSDKKPTPDPTPNPQSTVPSGWSGSSYYQ
                     NGTKVTSKWIFDKNYNSYFYLDASGNYVQNAWEGDYYLKSGGYMAKKEWIYDKNYGAY
                     YYLTPDGYYARNTWVGNYYLKSNGKMAKSEWIYDKNYSSYYYLTSEGSYARNTWVGDY
                     YLKSNGKMAKGEWIYDNNYSSYYYLTSEGSYARNTWVGDYYLKSNGKMAVSERTPDGY
                     QVDGSGKWISSEGTHTASSSQATSLGISLYNVNDAMTYIDIALPSTPSSSYQVAYDFA
                     LVDANTGSVSTQTQFTVTYNPSLRVFQTRHFFSATPDGSYRFVIKGKGQDGKVYDGQS
                     VVFRVKNHRFVG"
     gene            2474..3490
                     /locus_tag="VW325_000593"
     CDS             2474..3490
                     /locus_tag="VW325_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEU9734036.1"
                     /translation="MNKKKLLVCAVALSTVGVVSTIDTAGVLPFSAQTVHAEITRTEN
                     HEVKSKITPGSENGSIYVDMTVENVPVHYHVYIYADIVNAKTGQIPRKDTFNERFDRS
                     SVPITKQGWTNFANVPDGEYYLQFHGEDDYRSVLYKFIGKSATFTVKDQKYVNPLDSS
                     TPKETEKPTATETSKPATNSSSGQTGWSGSSYYKAGVKVVNQWIFDVQYNSYFYLNAS
                     GNFVQNAWSGSYYLKSGGYMAKSEWIYDNNYQSYYYLTAEGSYARNAWSGSYYLKSDG
                     KMAKSEWIYDSSYQSYYYLTSEGSYARNTWVGDYYLKSDGKMAVNERTPDGYKVDGSG
                     KWVR"
     gene            3631..5193
                     /locus_tag="VW325_000594"
     CDS             3631..5193
                     /locus_tag="VW325_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734037.1"
                     /translation="MKKFLLATTLLLTLVLGGCQYLPWNKSAETPSDSTSETSVATPE
                     IDYSSYNKVLDDYEKVAKGTLPTGMDVNPLASQVKSSQGFYTDVVYHKTDLNNDGVDE
                     LLLALEMKSGEKSLLDIRTLKDEKVIRLTNQENRLDQIGERMTVGILPDNSLLYRGAG
                     TATSHIYAHYQFSEDGQSLVKDKEAQELADLGVGSPISLETLSWKSVSDKLPGGSSAD
                     KGKPSVDYSPYNSILKDYEFILDYKSEVKDNINPTANIYAGMAERSQAFTYAMVDMDK
                     NGIDELIVSSKNGSEILDIFTLKDQKVIRLTNAENKMQMLGTRFKAYLLENGNLYTQW
                     NPFAGDPQTIHTIWKLNSSGDKLEKVKEAKTEDEVSPGTRMNLSSLTWKSVTEKFAAT
                     ASSTSKTASGKMDINAIKNGDFSSIEGRWSNGHGGEDLVFSKDGLIAPEKFKVDLTES
                     EFVNGFLRARYSAPDFGIFIQFVPAGVRIPDGTSRGELVTDASDISKDRIIFHAAFPL
                     HGDPRQFLYRVE"
     regulatory      5397..5485
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5576..6898
                     /locus_tag="VW325_000595"
     CDS             5576..6898
                     /locus_tag="VW325_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9734038.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVVLLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     gene            7116..7664
                     /locus_tag="VW325_000596"
     CDS             7116..7664
                     /locus_tag="VW325_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9734039.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            7774..8673
                     /locus_tag="VW325_000597"
     CDS             7774..8673
                     /locus_tag="VW325_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9734040.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEMAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(8698..9972)
                     /gene="serS"
                     /locus_tag="VW325_000598"
     CDS             complement(8698..9972)
                     /gene="serS"
                     /locus_tag="VW325_000598"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9734041.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(10186..10557)
                     /locus_tag="VW325_000599"
     CDS             complement(10186..10557)
                     /locus_tag="VW325_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9734042.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(10560..11924)
                     /locus_tag="VW325_000600"
     CDS             complement(10560..11924)
                     /locus_tag="VW325_000600"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9734043.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLTAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            12249..13034
                     /locus_tag="VW325_000601"
     CDS             12249..13034
                     /locus_tag="VW325_000601"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9734044.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            13128..13562
                     /locus_tag="VW325_000602"
     CDS             13128..13562
                     /locus_tag="VW325_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9734045.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            13562..14536
                     /locus_tag="VW325_000603"
     CDS             13562..14536
                     /locus_tag="VW325_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9734046.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            14597..14821
                     /locus_tag="VW325_000604"
     CDS             14597..14821
                     /locus_tag="VW325_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9734047.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            14940..15914
                     /gene="fabK"
                     /locus_tag="VW325_000605"
     CDS             14940..15914
                     /gene="fabK"
                     /locus_tag="VW325_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9734048.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            15907..16827
                     /gene="fabD"
                     /locus_tag="VW325_000606"
     CDS             15907..16827
                     /gene="fabD"
                     /locus_tag="VW325_000606"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9734049.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEATPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            16861..17592
                     /gene="fabG"
                     /locus_tag="VW325_000607"
     CDS             16861..17592
                     /gene="fabG"
                     /locus_tag="VW325_000607"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9734050.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGTFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            17614..18849
                     /gene="fabF"
                     /locus_tag="VW325_000608"
     CDS             17614..18849
                     /gene="fabF"
                     /locus_tag="VW325_000608"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9734051.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEILYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            18852..19337
                     /locus_tag="VW325_000609"
     CDS             18852..19337
                     /locus_tag="VW325_000609"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9734052.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            19334..19756
                     /gene="fabZ"
                     /locus_tag="VW325_000610"
     CDS             19334..19756
                     /gene="fabZ"
                     /locus_tag="VW325_000610"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9734053.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            19768..21135
                     /gene="accC"
                     /locus_tag="VW325_000611"
     CDS             19768..21135
                     /gene="accC"
                     /locus_tag="VW325_000611"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9734054.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            21172..22038
                     /locus_tag="VW325_000612"
     CDS             21172..22038
                     /locus_tag="VW325_000612"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9734055.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            22035..22802
                     /locus_tag="VW325_000613"
     CDS             22035..22802
                     /locus_tag="VW325_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9734056.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKSLEELLEERYQRFRKY"
     gene            22960..23144
                     /gene="briC"
                     /locus_tag="VW325_000614"
                     /pseudo
     CDS             22960..23144
                     /gene="briC"
                     /locus_tag="VW325_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
     gene            23162..23611
                     /locus_tag="VW325_000615"
     CDS             23162..23611
                     /locus_tag="VW325_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734057.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIELYVTIILGLGADVYSL
                     ILTMVLVGSFLLLIWRLKKKKSWNWSFVFSLFATYVVYQILGNFWARYAHLINHRNIH
                     DEYFTVVLSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            23633..23861
                     /locus_tag="VW325_000616"
                     /pseudo
     CDS             23633..23861
                     /locus_tag="VW325_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(23910..24332)
                     /gene="nusB"
                     /locus_tag="VW325_000617"
     CDS             complement(23910..24332)
                     /gene="nusB"
                     /locus_tag="VW325_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9734058.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(24325..24714)
                     /locus_tag="VW325_000618"
     CDS             complement(24325..24714)
                     /locus_tag="VW325_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9734059.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(24736..25296)
                     /gene="efp"
                     /locus_tag="VW325_000619"
     CDS             complement(24736..25296)
                     /gene="efp"
                     /locus_tag="VW325_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9734060.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(25425..26867)
                     /gene="gatB"
                     /locus_tag="VW325_000620"
     CDS             complement(25425..26867)
                     /gene="gatB"
                     /locus_tag="VW325_000620"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9734061.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(26867..28333)
                     /gene="gatA"
                     /locus_tag="VW325_000621"
     CDS             complement(26867..28333)
                     /gene="gatA"
                     /locus_tag="VW325_000621"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9734062.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(28333..28635)
                     /gene="gatC"
                     /locus_tag="VW325_000622"
     CDS             complement(28333..28635)
                     /gene="gatC"
                     /locus_tag="VW325_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9734063.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            28848..30392
                     /locus_tag="VW325_000623"
     CDS             28848..30392
                     /locus_tag="VW325_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9734064.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            30834..31319
                     /locus_tag="VW325_000624"
     CDS             30834..31319
                     /locus_tag="VW325_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU9734065.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFEGLSYAAFFRYFIPKYVSESRVL
                     YLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENITQDL
                     I"
     gene            31435..31623
                     /locus_tag="VW325_000625"
     CDS             31435..31623
                     /locus_tag="VW325_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9734066.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            31780..32145
                     /locus_tag="VW325_000626"
     CDS             31780..32145
                     /locus_tag="VW325_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9734067.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            32148..33815
                     /locus_tag="VW325_000627"
     CDS             32148..33815
                     /locus_tag="VW325_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9734068.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMSAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            34015..35715
                     /locus_tag="VW325_000628"
     CDS             34015..35715
                     /locus_tag="VW325_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9734069.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            35708..36184
                     /gene="ilvN"
                     /locus_tag="VW325_000629"
     CDS             35708..36184
                     /gene="ilvN"
                     /locus_tag="VW325_000629"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9734070.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            36250..37272
                     /gene="ilvC"
                     /locus_tag="VW325_000630"
     CDS             36250..37272
                     /gene="ilvC"
                     /locus_tag="VW325_000630"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9734071.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVTEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            37379..37615
                     /locus_tag="VW325_000631"
     CDS             37379..37615
                     /locus_tag="VW325_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734072.1"
                     /translation="MLADQNKADWSYDENTVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSSFERAGATKSARYIAKWILRNKKH"
     gene            37853..38209
                     /locus_tag="VW325_000632"
     CDS             37853..38209
                     /locus_tag="VW325_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9734073.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            38259..39509
                     /gene="ilvA"
                     /locus_tag="VW325_000633"
     CDS             38259..39509
                     /gene="ilvA"
                     /locus_tag="VW325_000633"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9734074.1"
                     /translation="MLSSKDIIKAHKILNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            40393..40608
                     /locus_tag="VW325_000634"
     CDS             40393..40608
                     /locus_tag="VW325_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734075.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
     gene            complement(40717..41457)
                     /locus_tag="VW325_000635"
     CDS             complement(40717..41457)
                     /locus_tag="VW325_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9734076.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(41457..43022)
                     /locus_tag="VW325_000636"
     CDS             complement(41457..43022)
                     /locus_tag="VW325_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9734077.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            43160..45049
                     /locus_tag="VW325_000637"
     CDS             43160..45049
                     /locus_tag="VW325_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9734078.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSTAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
BASE COUNT        13354 a         8310 c        10275 g        13412 t
ORIGIN      
        1 gggggaattc tgcttgaaga ttatcatggt tttaagaaat gataaattgg atggatagga
       61 gagcaggaag ttatatgaga acaaacattt taaaattaag tcaattcttg aatattttct
      121 atttaaaaat tgctttacta atattctcgt gcttgtattt tatcgctcca tggttcattg
      181 tattaaagga ttttgaaaca tataaagttc aatatccagc tgttattgga atagctgagt
      241 ttatcgtttt aacatctttt ttagtttttg tatatatttc accaatcttc tttgtgattt
      301 ttctatataa aataattgtg ggcagtatta atgtaagacg ctgtttgata aagataaaga
      361 attacaaatt ctatatgatt gttttgaata gaataccact acatacattt atggtttatt
      421 tttcatcagt attgttatgg ataatgtatc ttacaaaacc agtggatgtg agtaatgctt
      481 tacctttgcc aattatctta cctcttgaaa agataaatcc cttctatatg attctaggat
      541 attggctctt aaataagcga tgtgaatatc agttgcatca gtacctttat cctgtaatga
      601 gagggagttc tcatgacggt agaatttatg cactaccaga ggaacttttt atctggaata
      661 ggaatagaag tcaaaaaatt ggtaagtgga aacgagatag aaggtacaat acgaccaccg
      721 atctagtcga agctcaaatt ggttatagta agtttccgtt ttttttgcta gcaacaaaat
      781 ggtcagtgga tccttatgat caaaattgga caataggaga aaaggcatat ttgacaagta
      841 ggtggaaatg ataaaataga aagtctttag tctaataact aggaaaaaga ttaagtatac
      901 ttttttcata aaaatttcat ttgctaatca aataaagtat aaacacagat actcttttca
      961 taaatatagt agagtccatc ccttactgta agcctaaata ataaaaattt aaaggaggtt
     1021 ttgataatga agtcaaaaac agttttagta accagcctag ccgctgtaac atttggtctt
     1081 gtgtctcttg tagatacagc aggagctcta cctttttcag caaaaacggt acaagctgac
     1141 acacaaacgc tgactacagc tgcaaaatat tatattcttg ttccgaagga ttcagaagta
     1201 tttaaaaatt ggagaggtac tgctaatcgt cccattattt gggccacaaa aaccactcca
     1261 gaattatctt atttccaaga atctgctcgc aactatattg ctaatgagaa taagtctggt
     1321 gccaattaca tagagtggaa gggaactgta gaagagttta aagaggcgat taagaatctg
     1381 acggataaaa aactaactcc aaatccagct ccaatcccac aatcggataa aaaaccaacc
     1441 ccagatccaa ctccaaatcc acaatctaca gttccgtctg gttggtcagg atcatcttac
     1501 taccaaaatg gtactaaagt cactagtaag tggattttcg ataagaacta taattcctac
     1561 ttctacctag atgcttcagg taattatgtt caaaatgcat gggagggtga ttactatctt
     1621 aagtcaggtg gctacatggc taagaaggag tggatctatg ataagaacta tggagcttat
     1681 tattacttaa ctccagatgg ctactatgct cgcaatactt gggtgggcaa ctattatctc
     1741 aagtcaaacg gtaagatggc taaaagcgag tggatctatg ataagaacta cagttcatat
     1801 tactacctaa cgtcggaagg aagttacgcc cgtaatactt gggtaggtga ttattatctc
     1861 aagtcaaatg gaaagatggc taaaggtgag tggatttatg acaataacta cagctcttac
     1921 tattacttaa catcggaagg aagttatgct cgtaatacat gggtaggtga ttattatctc
     1981 aagtcaaatg gaaagatggc agttagcgaa cgtacaccag atggctacca agtggatgga
     2041 tcaggcaagt ggattagttc tgagggaact catacagcaa gcagttcaca agcaacatca
     2101 ttaggaataa gcctttacaa tgtcaatgat gctatgacct atattgatat cgctctacct
     2161 agcactccat cctcatctta tcaagtagcc tatgattttg ccttagttga tgcaaataca
     2221 ggttcagttt ctacacaaac ccagtttact gtgacctaca atccaagctt acgtgtcttt
     2281 caaactcgtc acttctttag tgccactcca gatggtagtt atcgctttgt tatcaaagga
     2341 aaaggacaag atggcaaggt atacgatggc caatcagttg tctttcgtgt aaagaatcat
     2401 cgttttgtcg gatagatgag tgataggttt taaacaaata aatattaaaa ataaaataaa
     2461 ggaaatgaat aacatgaaca aaaagaaatt attagtatgt gctgtggcac tttcaacagt
     2521 cggagtagta tcaacaatcg acacagcagg agttctccct ttttcagcac aaacagtgca
     2581 tgcggaaatt actcgtaccg agaatcatga ggtaaaatct aagattacac caggatcaga
     2641 aaatggaagt atctatgtgg atatgactgt tgaaaatgta cctgtacact atcatgtata
     2701 tatatatgca gatattgtaa atgcaaaaac agggcaaatt cctagaaaag atactttcaa
     2761 tgaaaggttc gatagatcat ctgttccgat tacaaagcaa ggttggacta attttgcaaa
     2821 tgttccagac ggtgaatatt acttacaatt tcatggagaa gacgattaca gaagtgtttt
     2881 gtataaattt attggcaaat cagcgacatt cactgttaaa gatcaaaaat atgtcaatcc
     2941 tctagactct tcaactccta aagaaacgga aaaaccaaca gctactgaaa cttcaaaacc
     3001 agctactaac tctagctcag gtcagactgg ctggtcaggt tcttcttact acaaagctgg
     3061 tgtgaaagta gtcaatcaat ggatttttga tgttcagtac aattcatact tctacctcaa
     3121 tgcttcaggt aactttgttc aaaatgcttg gtctggaagt tactacctca agtcaggtgg
     3181 ctacatggct aagagcgaat ggatttatga caataactat caatcatact attacttgac
     3241 agcagagggt agctatgcac gtaatgcttg gtctggaagc tactacctca aatcagatgg
     3301 taaaatggct aagagtgagt ggatctatga tagtagctac caatcttact attacctaac
     3361 atcagaagga agttacgccc gtaatacatg ggtaggtgac tactacctca aatcagatgg
     3421 taagatggca gtcaacgaac gtaccccaga tggctacaaa gtagacggct caggtaagtg
     3481 ggttcgctag aatgatagct tgtgagaaat ggcttataac aaataaatag aaacggaaaa
     3541 taatttgttt ataggcaaca ttttgataca ataaaaatag cgaaaggaat tggcacaggc
     3601 tggttccttt ctcttagata aaggagtaat atgaaaaagt ttttattagc cacaacactt
     3661 ttactgacac tagttctagg aggctgtcag tatttacctt ggaacaaaag cgcagaaaca
     3721 ccatctgatt caacatcaga aacatctgta gcgactccag agatagacta cagttcctat
     3781 aataaagttt tggatgacta tgaaaaagta gcgaaaggca ccttgccgac aggtatggat
     3841 gtcaatcctc tggctagtca agtgaagtct tctcaaggct tttatacaga tgtggtttac
     3901 cacaagacgg atttgaataa tgatggagtc gatgaacttt tacttgctct tgaaatgaag
     3961 agtggcgaaa agagtttact tgatattcgt acgctgaaag acgaaaaggt gattcgtttg
     4021 accaatcaag aaaatcgttt agaccagatt ggtgagagaa tgacggtcgg tatccttcca
     4081 gacaattctc tgctctatcg tggagctggt acagcaacga gtcacatcta tgctcactac
     4141 caatttagtg aagatgggca gtctttggta aaggataagg aagctcaaga actagcagat
     4201 cttggggtcg gttcgcccat ttctctagag accttaagct ggaagtcagt ttcagataaa
     4261 cttccaggag gaagcagtgc agataagggg aaaccaagtg tagattacag tccttataat
     4321 agcattttga aggattatga atttatttta gactataagt cagaggttaa ggataacatc
     4381 aatccaactg ctaatatcta tgcaggaatg gcagaacgca gtcaagcctt tacctatgca
     4441 atggttgata tggacaaaaa tggcattgat gaattgattg tctcttcgaa aaatggtagt
     4501 gagattttag atatttttac actcaaagat cagaaagtta ttcgcctcac caatgctgaa
     4561 aataaaatgc agatgcttgg aactcgtttc aaggcctatc tattagaaaa tggtaatctc
     4621 tatacccagt ggaatccgtt cgcaggagat ccacagacca ttcataccat ttggaaattg
     4681 aatagtagtg gggacaaact agagaaggtt aaagaagcta agacagagga tgaagtcagt
     4741 ccaggtactc gaatgaatct atctagtctg acctggaaat ctgtaacaga aaaatttgct
     4801 gcaacagcat cttcaactag caaaacagct tcagggaaaa tggatataaa cgccatcaaa
     4861 aatggagatt tttctagtat tgagggaaga tggagtaatg gtcatggagg agaagactta
     4921 gtctttagta aagatggctt gatagcacca gagaaattta aagttgacct tacagaatca
     4981 gaatttgtaa atggtttttt acgagctcgt tattcagcac cagactttgg aatctttatt
     5041 cagttcgtac cagcaggtgt tagaataccg gatgggacat ctaggggtga gttggtcacg
     5101 gatgcttcgg atattagtaa agatcgaatc atcttccatg ctgcatttcc gctacatggt
     5161 gatccaagac aattcttata tcgtgtggaa taagagtgaa acagcttgat ttaataacta
     5221 atagcacccc acgcacatgg gaatgctcaa tccttcttct acagagtcaa ataagatttt
     5281 tccaaaacac attgacaaaa gccaacattt tttgtaaaat aagaatcaat taaataccaa
     5341 caccgaatga agtttaatag aagtggggaa tcgtttgatt ttccatgact gtaaatggac
     5401 ggaactctgg agagaccgta aaggcaccga aggggcaagg caggcaactg ctcaaactct
     5461 caggtaaaag gacagagcta ggatagaccg ctttttagca tttatctaag cattccagag
     5521 tacatgtatc ttgcatgtgc tctttctttt ggggttgaaa cgataggaga aggaaatgtt
     5581 agaattgctt aaatcaatcg atgcttttgc ttggggaccg cccctcttga ttttattggt
     5641 cggaacaggg atttacctaa ccatgcggct aggactcttg caggttttgc gtctgcccaa
     5701 ggcctttcag cttattttta tccaggataa gggacatggt gatgtatcca gttttgcagc
     5761 tctgtgtaca gccttggcat caactgttgg aacaggaaat atcataggag ttgcgacggc
     5821 tatcaaggtt ggtggaccag gagctctatt ttggatgtgg atggcggctt tctttggaat
     5881 ggctaccaag tatgcggaag gactcttggc catcaaatac cgcaccaagg acgaccatgg
     5941 tgcagtagcg ggaggtccca tgcactatat ccttctaggg atgggagaaa agtggcgacc
     6001 acttgctgtt ttgtttgcag tagcaggagt attggttgtt ctcttgggaa tcggaacctt
     6061 cacccaagtc aactcgattg cagaatctat ccaaaataca acgacgattt cgccagccat
     6121 cacagctctc gtcttgtctg tctttgtagc gattgcagtc tttggtggac tcaagtctat
     6181 ttctaaggtt tcaactactg ttgttccttt tatggccctc atttatatct taggaactct
     6241 tacagttatt ttctttaata tcggaaaaat ccctggcaca atcgctttag tctttacctc
     6301 agcttttagt ccccttgctg cggtaggtgg atttgctggt gctagcgttc ggatggctat
     6361 tcaaaatggt gtggcgcgtg gtgtgttctc aaacgaatcg ggtctgggtt ctgctcctat
     6421 tgcagctgca gctgccaaga caaatgaacc agtagagcaa ggtttgattt ccatgacagg
     6481 aacctttatt gataccctca tcatttgtac tctaactggt ttgaccatct tggtaactgg
     6541 agtttggagc ggtgacttga atggggttgc cttgactcag tcagctttct caacagtctt
     6601 ttcacacttt gggcctgctc tcttgaccat cttccttgta ctttttgcct ttacaacgat
     6661 tctaggttgg aactattacg gagaacgctg ttttgagttc ctctttgggg ttcgctttat
     6721 ctggctctac cgtgtggttt ttgtgctcat ggtcttgtta ggaggattta tcgagttgga
     6781 tatggtctgg attatcgcag atatcgttaa cgccttgatg gctctgccaa acttgattgc
     6841 cctcttggtc ttgtcgccag tcgttattgc tgaaactaaa aagtattttg acaaataatg
     6901 gaatcacaca gctcgtgtga tttttttgcc tataaataat ttttatcagc ctgatatata
     6961 atatatatta tgcaagtgat tggaagtgtg ataaactaat ataacaacga aaatcttatg
     7021 agagaataaa gctttatctt agataaactt aggaatcagg attttataag attttccaat
     7081 aatattagtg tcaacaataa gaaaagaggt atcttatgac aacatttact atccatacag
     7141 tagagtcagc accagcagaa gtgaaagaaa ttcttgaaac agtagaaaaa gacaacaatg
     7201 gctatattcc caacctaatc ggtctcttgg ccaatgcccc gactgtttta gaagcctacc
     7261 aaattgtctc atctatccac cgtcgcaaca gcctgacacc cgttgagcgt gaagtggtgc
     7321 aaatcacggc agccgtgacc aatggttgtg ccttctgtgt cgcaggtcac acagcctttt
     7381 ccatcaaaca aatccagatg aatgatgact tgattcaagc tcttcgcaat cgtactccaa
     7441 ttgaaacaga tcctaaattg gataccctag ctaagtttac cttggcagtt atcaatacca
     7501 agggtcgtgt aggagatgaa gccttgtctg agtttttaga agctggctac actcaacaaa
     7561 atgccttgga tgtggttttt ggtgtcagcc tagcaatcct ctgtaactat gccaacaact
     7621 tagctaatac accaattaat ccagaattgc aaccttatgc ctaattcata tctgagtaaa
     7681 atgaagtctg aaataatcgg acttcgtttt tttaaatcct catttaagcg cttttatgtt
     7741 atactgaaac taacatgatt attggaggtt aggatgaaaa aactcccctt agtattttca
     7801 ggttgtttgc taggtttggc aggagctgga aatcttattt tagatacgtt gccagttcta
     7861 tcccatcttt ttagtctgac tggtttggtt ttatggattt actttctaat tctgcatctc
     7921 tttaattgga aagaaaccaa gcaagaattg accaagcccc ctcttttgtc aggaatggca
     7981 acctttccta tggctgggat gattttatcg acctatgtct ttcgcgtatt ctcttatctt
     8041 cctttggtag cacaagggat ttggtggttt tcatttctct tggatttgac cttgattgct
     8101 ggttttacca tcaagtttgc ttgtccaggg cggagggttc atgccactcc aagttggacg
     8161 gttctctatg tggggatagc agtggctgcc ttgacctatc ctctggtagg tattatcgaa
     8221 atggcctatg cgaccctgag ttttggtttt ctcttgacct tctatctcta tccccttatt
     8281 tatagcgatt taaagaaaca tccactccca ctagccttgc ttggacaaga aggaatctac
     8341 tgtgctcctt tctctctact cttggcttct ctagttcgag taggaggaac cagcctgccg
     8401 acttgggtct tgattgtcat gattttggct tctcaatcct tctttttctt tgttttaact
     8461 cgtctgccca acattttaaa acaaggtttt caaccagcct tctcagccct caccttccca
     8521 accattatca cagcgacctc gctcaagatg gctcagggaa ttttgaaact tccatttctg
     8581 gattacctgg tattggctga aaccattata tgcctaacta ttttattctt tgtactaggt
     8641 gcttatctga tttggttacg aaaaaaggtc tagctagaaa tagctaaacc ttatttttta
     8701 tggtttgatg acttcagctc cacccatgta tggacgaagt gcttctggga tggtcacaga
     8761 accatcttca ttttggtaat tttcaagaat tgcagccact gtacgtccaa ctgcaagtcc
     8821 agaaccgttc aaggtatgaa ggagtttcac cttgccatct gcttcatcac ggtaacggat
     8881 ttgggcacga cgggcttgga aatcttctgt gtttgaacag cttgagattt cacggtaatt
     8941 gttttgtgct ggaatccaca cttccaagtc gtaagtcttc gcagctgaga agcccatatc
     9001 tccagtagag agagcaacga cacggtatgg aaggttgagt ttttgaagaa tgttttcagc
     9061 gttggctgtc attttttcca attcttcgta agattcttct ggtttggcaa atttgaccat
     9121 ttcaaccttg tggaattggt gcaaacggat caagccacgc gtatcacgac cggcagaacc
     9181 agcctcagaa cggaatgacg gactcatggc agtgaagtag attggaagat ctttgccgtc
     9241 taagatttca tcacggtagt agtttgtcag aggaacttca gcagttggaa tcaagacaaa
     9301 gttggtatcg ctgagttcaa aagtatcttc cttaaatttt ggatactgac cagtaccaaa
     9361 catagaatca tggttgacta tgtaaggtgt gatgacttca gtatagcctt cttttccatg
     9421 ttcatccaac ataaagttgt agatagcacg ttccaaacga gcaccgaggc ctttatagaa
     9481 gaggaagcga gcgcctgtta ccttaccacc gcgttcccag tcaagaatac caaggtcttc
     9541 accgagatcc cagtgagctt taggttcgaa gtcaaactcg cgtggagtac cccaacggcg
     9601 aacttccaca ttgtcgtctt cgtcagcccc aacaggaacg ctgtcagctg ggatatttgg
     9661 aagagtcgtt gtaaattctg tcaatttagc atcgatttct gccaattcag catccaaggc
     9721 tttaacctca gcagatagat tttgcatggc agcaatcttg tcatctgtat tttccttgtt
     9781 gcgcttagct tgggcaatct cagcagaaac tgtgttacgt tctgctttga gagtttcaac
     9841 cttgaccaag atgttacgac gtttagcatc gatttctttc atttcattca agacagcagc
     9901 atctacacca cgtgtagcta atttttctgc gacagcttca aaatctgtac gaatacgttt
     9961 gatatctaac ataagaactc ctttatgaaa aaagcacgcc tgacaaaacg ttggagtggc
    10021 agggccacgg ttccatccaa cttcacaggt gtgcacttga ttgtgtatgt aattgttact
    10081 aacggtagaa tttcacctat ccctcctatc tgctcgcagt acccgcagac tttctgaaag
    10141 aagaagataa cctacttatc cgttgctatg attatactaa agtttctact tttttgcaaa
    10201 tagattttta aatttttggc taattgtctg aatcagggtc ggaagtttga cgaccttgtc
    10261 attgcctagt ttttcgcgtg caattttgag aatggcacct gagtcttttg aagcaaagag
    10321 gaattttcct ttgtctgtaa agacttcgaa gtggcggctg attttgcgtc cagtgacatt
    10381 ggctccaatc tgattgatat ggctccaagg aatctggata aattgttcga cattgacatc
    10441 tgggtaaaat tccaaagcct gatctccgac aaggaatttc ccaactttcc cagcgataga
    10501 gaggtaggaa gtgcctgtcg tactgaggag tactgttttg ttaagtgatt gggccatgct
    10561 tagtcttcct tactttctcc aaaaaaggca ttgtagaggg ctttaattgc tgctttctct
    10621 tggtctttat tgacaacaaa cataatagaa acttcactag aaccttgaga catcatctgg
    10681 atgttgattt tgttttcaga tagagcgcgt gtcgcagtag cagtcactcc gatgtggctc
    10741 ttcatttttt caccaacaat cataatgata gaaaggtcgt gttcgatttc tgcatgatct
    10801 actttagcct tttgaaccaa ctgacgcagg atttcttctt ccttgatggg agttagttgg
    10861 cgagaacgga gaatgataga aagatcgtcg atacctgttg gcatatgttc ccaaccgatg
    10921 ttaagttctt ccaggatttg cagaaccttg cggccaaatc caacctcacg gttcatgagg
    10981 tatttcgaca tgttaatgct gacaaagcct gagtcaccag caattcccac aactggaaat
    11041 tcatcattac tgtgttttag aacgatacga gtacctggat ggtcagggtt gttggtattc
    11101 ttgataacca gaggaatttt tccacggtag gcaggaagaa gagcctcgtc atgaaggact
    11161 gagaagcctg cataggccaa ctcgcgcatt tcacggtagg tcaactcagg aatcgagtgt
    11221 ggttggtgga taataccagg gtgggctgca aagataccat caacgtccgt aaagttttca
    11281 tagaggtcgg ctttgacacc agcagcaatg atagaacctg taatatcaga acctccacgt
    11341 gagaaagtac agatttgatt ttccttagtg acaccaaaga aaccaggaat gacaaggact
    11401 tcattggtgt ttgtcaattc ttcaatcttg tcataacttg atggaatgat gcgagcgtga
    11461 ccaggttcac ttgtgaccac aatcccagct tctctagggt gcatatagcg tgcatcgata
    11521 ccattttggt taaagtaggc agcagtcaat ttggcattgt tattttcacc ggctgctagg
    11581 aaagtatcgt agagaaattc attttcttca ataggaagag tggccaaggc gtgaatgctt
    11641 ttagaaattt tttctagcac agctggtttt agtcccaatt cactaaccat agcagcatag
    11701 cggtcgataa tccagctttg gctcttgcta atatcgttac ccgcaacata gtcgcggtag
    11761 tatttaatca gggcatccgt aaccttagta tcttcagcat tgcgtttacc aggcgcagaa
    11821 acgactacaa aacgacgctc tgaatcgctt ttgacgatgt ttaaaacttt ttctaattga
    11881 ctagcagagg caagagaact acctccaaat ttaacaactt tcataagaac tcctaaagta
    11941 agtattttat acgattatag cagaaagaaa ggcttttttc aatgaagaaa ataagatgct
    12001 ttctagccta aagcgagcct ttcttatcgg ctgagacact ttcagtcgat tttttcttgc
    12061 tttctgctcc taaaaaagat atactttgac agtgaaataa tttaacactc aatgaaaatc
    12121 aaagagcaaa ctaagaagct agacgaagtc agctcaaaat actgttttga ggttgcagat
    12181 ggaagctgac gcggtttaaa gagattttcg aagagtataa actgcttata aaataaaaag
    12241 gagccctgat ggaacacatt atttatcagc ttgaagagga cttggcaatc cttaccttga
    12301 accgtcctga ggtcgcaaat ggttttcata ttcccatgtg tgaggagatt ttagaagctc
    12361 tgactttggc agaagaaaat ccagctgtgc attttatctt aatcaatgcc aatggaaagg
    12421 tcttctcagt tgggggagat ttggtagaga tgaagcgggc agtggatgag gatgatattc
    12481 catcattgac aaaaatcgca gagttggtca atacgatttc ttataaaatc aagcaaatag
    12541 ccaaacctgt tttaatggaa gttgacggtg ctgttgcagg tgccgcagcg aatatggctg
    12601 ttgcggcaga tttctgtttg gcgacggata aggctaagtt tatccaagcc tttgttggtg
    12661 ttggtttggc tccagatgca gggggaattc atctcttgag ccgcagtatc ggtgtgacgc
    12721 gtgctgctca attagctatg acaggagagg ctttaacggc agaaaaagcc ctagaatggg
    12781 ggttggttta ccgcgtttct gaagctgaaa aacttgaaaa gacgagagaa cagcttctta
    12841 aaaaattaag acgtgcttca agtaattcct atgctgccat taagaagttg gtttgggaga
    12901 gccagtttaa agactggcag ggttacgcta ctttagaact gaacctacag aaatccttag
    12961 ctcaaacaga ggatttcaaa gaaggagtgc gagctcattc ggagagaaga agacctaaat
    13021 ttacaggaaa ataaaaaata cttgcatcat tctttgaatt ttgatataat tctttctgtc
    13081 aaatgttttg attgtaaaag ttttttgaag aaggagggat atgaaaaatg gactaccaac
    13141 gaatcaatga atatttaaca tctatattta acaatgtcct tgtaattgag gaagtgaact
    13201 tgagaggtag tcgttttaag gatatctcca tcaaagaaat gcatacgatt gatgtcatcg
    13261 gtaaggctcc agacgtgact ccaagtcaag tgtcaaaaga gttgatggta actcttggaa
    13321 ctgttacgac aagtttgaac aatttagagc gtaagggcta cattgagcga gttcggtcag
    13381 aacaggatcg tcgtgtggtg catctgcatt tgacaaagaa gggtcgcttg attcataggc
    13441 tgcataaacg cttccacaag gccatggtag aaaaaattat tgatggcatg agcgaggaag
    13501 aaattgctgt catgggtaaa ggtttgacta atctttacca atttttggag gatttgaaat
    13561 aatggctttt gcaaaaataa gtcaggttgc tcattatgtg ccagagcaag tggttacaaa
    13621 tcacgacttg gctcagatta tggataccaa tgatgagtgg atttcaagtc gaacgggaat
    13681 acgacaaagg catatttcaa gaacagaatc taccagtgat ttggctacag aggttgctaa
    13741 gaaactgatg gcaaaagctg gaataacagg agaagaactg gattttatca tcctagctac
    13801 cattactcca gattcgatga tgccctctac agctgctcgt gttcaagcta atattggtgc
    13861 taataaagcc tttgcttttg acttaaccgc ggcttgcagt ggatttgtat ttgctctttc
    13921 aactgctgaa aagtttatcg cttctggtcg ctttcaaaaa ggcttggtga ttggtagtga
    13981 aaccctctct aaggcagtcg attggtcgga tcgatcaaca gctgtgttgt ttggagatgg
    14041 tgctggtggt gtcttgttag aagctagcga gcaagagcat ttcttagctg agagtctcaa
    14101 tagcgatgga agtcgcagcg agtgtttaac ttatgggcat tcaggtttgc attctccatt
    14161 ttcagatcaa gaaagtgcag attcgttttt gaagatggat ggacgcacag tctttgattt
    14221 tgccattcga gatgtagcca agtctatcaa gcagactatt gatgaatctc ctatagaggt
    14281 gacagacttg gattatctgc tacttcatca agccaatgac cgtattttgg ataagatggc
    14341 tagaaaaatt ggtgttgacc gagccaaact tccagccaat atgatggaat atggcaatac
    14401 cagtgcagcc agtatcccga ttttactttc agagtgtgta gaacaaggtc tcatcccttt
    14461 agatggtagc cagactgttc ttctatcagg cttcggtgga ggcttgacct ggggcacgct
    14521 cattcttaca atttaggtaa tcatgtggtg aacacattgt tataaaaaaa actatttatt
    14581 ttaaaggagt cctatcatgg cagtatttga aaaagtacaa gaaattatcg ttgaagaact
    14641 tggaaaagac gcatcagaag taacacttga atcaactttt gatgatttgg acgcagattc
    14701 attggacttg ttccaagtaa tctcagaaat cgaagatgct tttgatatcc aaatcgaagc
    14761 agaaaatgac ttgaaaacag ttggtgactt ggttgcttac gttgaagagc aagcaaaata
    14821 agcagaatat tagtagaagg agtagggaga aacccactcc ctcttgttta ggtaatagtt
    14881 tgactgtcaa attatggtga aatcgaacta ttacgtaagc aaggaaggtt ggaggcacta
    14941 tgaaaacgcg tattacagaa ttattgaaga ttgactatcc tattttccaa ggagggatgg
    15001 cctgggttgc tgatggtgat ttggcagggg ctgtttccaa ggctggagga ttaggaatta
    15061 tcggtggggg aaatgccccg aaagaagttg tcaaggccaa tattgataaa atcaaatcat
    15121 tgactgataa accctttggg gtcaacatca tgctcttatc tccctttgtg gaagatatcg
    15181 tggatctcgt tattgaagaa ggtgttaaag ttgtcacaac aggagcagga aatccaagca
    15241 agtatatgga acgtttccat gaagctggga taatcgttat tcctgtcgtt cctagtgtcg
    15301 ctttagctaa acgcatggaa aaaatcggtg cagacgctgt tattgcagaa ggaatggaag
    15361 ctggggggca tatcggtaaa ttaacaacca tgaccttggt gcgacaggta gccacagctg
    15421 tatctattcc tgttattgct gcaggaggaa ttgcggatgg tgaaggtgct gcggctggct
    15481 ttatgctagg tgcagaggct gtacaggtgg ggacacggtt tgtagttgca aaagagtcga
    15541 atgcccatcc aaactacaag gagaaaattt taaaagcaag ggatattgac actacgattt
    15601 cagctcagca ctttggtcat gctgttcgtg ctattaaaaa tcagttgact agagattttg
    15661 aactggctga aaaagatgcc tttaagcagg aagatcctga tttagaaatc tttgaacaaa
    15721 tgggagcagg tgccctagcc aaagcagttg ttcacggtga tgtggagggt ggctctgtca
    15781 tggcaggtca aatcgcaggg cttgtttcta aagaagaaac agctgaagaa atcctaaaag
    15841 atttgtatta cggagccgct aagaaaattc aagaagaagc ctctcgctgg acaggagttg
    15901 taagaaatga ctaaaacagc ctttttattt gctggtcaag gtgcccagta tctagggatg
    15961 ggacgggatt tctatgatca gtatccgatt gttaaagaaa cgattgatcg agcgagtcag
    16021 gtgctcggtt atgatttgcg ttatctcatc gatacggaag aggacaaact caatcagacc
    16081 cgctatacgc aaccagccat tctagcgact tcggttgcta tctaccgttt attgcaagaa
    16141 aagggctatc agcctgatat ggttgctggt ttgtctcttg gagaatactc tgccttggtg
    16201 gcaagcggcg ccttggattt tgaagatgcg gttgccttgg tagctaagcg tggagcctat
    16261 atggaagaag cgactcctgc tgactctggc aagatggtag cagttctcaa tacgccagta
    16321 gaggtcattg aagaagcctg tcaaaaagct tctgaacttg gagtggttac tccagccaac
    16381 tataacacac ctgcacaaat cgtcattgct ggagaagtgg ttgcagttga tcgagcggtt
    16441 gaacttttgc aagaagcagg tgccaaacgc ttgattcctc ttaaggtgtc aggtcccttt
    16501 cacaccgctc tccttgagcc agctagccag aaactagctg aaactctagc tcaggtaagt
    16561 ttttcagatt ttacttgtcc cctagtcggc aatacagaag ctgctgtgat gcaaaaagag
    16621 gacattgctc agctcttgac gcgtcaggtc aaggaacccg ttcgtttcta tgaaagtatt
    16681 ggggtcatgc aagaagcagg cataagcaac tttatcgaga ttggaccggg gaaagtcttg
    16741 tcaggttttg ttaaaaaaat tgatcaaact gctcacttag ctcatgtgga agatcaagcg
    16801 agtttagtag cacttttaga aaaatagact aaaataagta gaagttttga aaggaaaaaa
    16861 atgaaactag aacataaaaa tatctttatt acaggttcga gtcgtggaat tggtcttgcc
    16921 atcgcccaca agtttgctca agcaggagcc aacattgtct taaacagtcg tggggcaatc
    16981 tcagaagaat tgctcgctga gttttcaaac tatggtatca aggtggttcc catttcagga
    17041 gatgtatcag attttgcaga cgctaagcgt atgattgatc aagctattgc agaactgggt
    17101 tcagtagatg ttttggtcaa caatgcaggg attacccaag atactcttat gctcaagatg
    17161 acagaagcag attttgaaaa agtgctcaag gtcaatctga ctggtacctt taatatgaca
    17221 caatcagtct tgaaaccgat gatgaaagcc agagaaggtg ctatcattaa tatgtctagt
    17281 gttgttggtt tgatggggaa tattggtcaa gctaactatg ctgcttctaa ggctggcttg
    17341 attggcttta ccaagtctgt ggcacgcgag gtcgctagtc ggaatatacg agtcaatgtg
    17401 attgctccag gaatgattga gtctgatatg accgctatct tatcagataa gattaaggaa
    17461 gctacactag ctcagattcc gatgaaagaa tttgggcagg cagagcaggt tgcagatttg
    17521 acagtatttt tagcaggcca agattatcta actggtcaag tgattgccat tgatggtggc
    17581 ttaagtatgt agcgaaagct agaggtgaaa agaatgaaac taaatcgagt agtggtaaca
    17641 ggttatggag taacatctcc aatcggaaat acaccagaag aattttggaa tagtttagca
    17701 actgggaaaa tcggcattgg tggcattaca aaatttgatc atagtgactt tgatgtgcat
    17761 aatgcggcag aaatccaaga ttttccgttc gataaatact ttgtaaaaaa agataccaac
    17821 cgttttgata actattcttt atatgccttg tatgcagccc aagaggctgt aaatcatgcc
    17881 aatcttgatg tagaggctct taatagggat cgttttggtg ttatcgttgc atctggtatt
    17941 ggtggaatca aggaaattga agatcaggta cttcgccttc atgaaaaagg acccaaacgt
    18001 gtcaaaccaa tgactcttcc aaaagcttta ccaaatatgg cttctgggaa tgtagccatg
    18061 cgttttggtg caaacggtgt ttgtaaatct atcaatactg cctgctcttc atcaaatgat
    18121 gcgattgggg atgccttccg ctccattaag tttggtttcc aagatgtgat gttggtggga
    18181 ggaacagaag cttctatcac accttttgcc atcgctggtt tccaagcctt aacagctctc
    18241 tctactacag aggatccaac tcgtgcttcg atcccatttg ataaggatcg caatgggttt
    18301 gttatgggtg aaggttcagg gatgttggtt ttagaaagtc ttgaacacgc tgaaaaacgt
    18361 ggagctacta tcctggctga agtggttggt tacggaaata cttgtgatgc ctaccacatg
    18421 acttctccac atccagaagg tcagggagct atcaaggcca tcaaactagc cttggaagaa
    18481 gctgagattt ctccagagca agtagcctat gtcaatgctc acggaacgtc aactcctgcc
    18541 aatgaaaaag gagaaagtgg agctatcgta gctgttcttg gtaaggaagt acctgtatca
    18601 tcaaccaagt cttttacagg acatttgctg ggggctgcgg gtgcagtaga agctatcgtc
    18661 accatcgaag ctatgcgcca taactttgta ccaatgacag ctgggacaag tgaagtatca
    18721 gattatatcg aagctaatgt cgtttatgga caaggcttgg agaaagaaat tctatacgct
    18781 atttcaaata cttttggttt tggaggccac aatgcagttc ttgctttcaa acgttgggag
    18841 aatagataag tatgaattta aacgatatta aagacttgat gactcaattt gaccagtcaa
    18901 gtttgagaga attttcttat aaaaatggga cggatgagtt gcagtttagc aagaatgaag
    18961 caagacctgt gcctgaagtt gcaactcaag tcgctccagc acccgttcta gcaacaccga
    19021 gtccagtagc tcctacatct gctccagcag agactgtagc agaagaagtt ccagctccag
    19081 ctgaagcaag tgtggctact gagggaaatc ttgtagagag tccacttgtt ggagtggttt
    19141 acttggctgc tggaccagat aaacctgcct ttgttacagt tggtgatagt gtcaaaaaag
    19201 gtcaaacatt ggtaattatc gaagccatga aagtcatgaa tgaaatccca gctcctaagg
    19261 atggtgtggt aacggaaatt ctcgtctcta acgaagaaat ggttgagttt ggtaaaggat
    19321 tggtacgtat caaatgatcg atattcaagg aatcaaagaa gctcttcccc accgttatcc
    19381 tatgcttcta gtggaccgtg tcttggaagt gagcgaggat accattgttg ctatcaaaaa
    19441 tgtgaccatt aacgaacctt tctttaacgg ccactttcct caatacccag ttatgccagg
    19501 tgttctgatt atggaagcct tggcgcaaac tgctggtgtg ttggagttat caaaacctga
    19561 aaataaagga aaactggtct tttacgctgg tatggacaag gttaagttca agaagcaagt
    19621 tgtaccaggc gaccaattgg ttatgacagc gacttttgta aaacgtcgtg gcaccatagc
    19681 tgtggttgaa gcaaaggctg aagtggatgg caagcttgca gccagtggta cccttacttt
    19741 tgcaattggg aactaaggag gttctccatg tttcgaaaaa ttttaattgc caatcgtggt
    19801 gaaattgcgg ttcgtattat ccgtgcggca cgtgaattgg ggattgcgac ggtagcggtt
    19861 tattcaactg ctgataagga agctcttcat acgctgttgg cagatgaagc agtttgtatt
    19921 ggtcctggca aggcaacaga gtcttatctc aatattaatg cagttctatc agctgcagtc
    19981 ttgactgagg cagaagctat tcaccctggt tttggatttc tcagtgaaaa ttccaaattt
    20041 gcgaccatgt gtgaagaagt aggtatcaag tttatcggtc catctggtca tgttatggat
    20101 atgatggggg ataagatcaa tgcgcgtgct cagatgatta aagcaggtgt gcctgttata
    20161 ccaggttcag atggagaagt gcataactct gaagaagctt tgattgttgc tgaaaaaatt
    20221 ggctatcctg ttatgctcaa ggcttcagca ggtggaggtg gtaaagggat tcgtaaggtt
    20281 gaaaaaccag atgacctcgt ttctgccttt gaaactgcct ctagtgaggc caaggccaat
    20341 tatggcaatg gtgccatgta catagaacgg gttatctatc cagctcggca cattgaggtt
    20401 caaatcctag gtgatgagca tggacatgtg attcacttgg gtgaacggga ttgttctctt
    20461 caaaggaata accaaaaggt tttggaagaa agtccctcga ttgcaatcgg aaaaacgctg
    20521 cgtcatgaaa taggtgctgc tgctgttcga gcggcagagt ttgttggcta tgagaatgca
    20581 ggaaccattg aatttcttct tgatgaagca agtagcaatt tctatttcat ggagatgaat
    20641 actcgtgttc aggtagaaca tccagtaaca gagtttgttt caggtgttga tatcgttaag
    20701 gaacagattt gcattgcggc aggtcagcct ttgtctgtta agcaagaaga tattgtccta
    20761 cgcggtcatg ccatcgagtg tcgtatcaat gcagaaaacc cagcctttaa ctttgctcca
    20821 agtccaggta agattactaa cctctatctg ccaagtggtg gagttggctt gcgcgtggat
    20881 tcagcagttt atccaggtta taccattccg ccttattatg atagtatgat tgccaaaatc
    20941 atagtacacg gcgaaaatcg ttttgacgcc ttgatgaaaa tgcaacgtgc cctctatgaa
    21001 ttagagattg aaggagtgca gaccaatgca gatttccagc ttgacctcat ttcagatcgc
    21061 aatgtcattg ctggggatta tgatacttcc ttcttgatgg aaaccttctt acctaaatat
    21121 caagaaaaag aataaaatga ctttaagagt ttaaaccgaa aaggggaatc aatggctcta
    21181 tttagtaaaa aagataagta tattcgaatc aatcccaatc gttcggttag ggaaaaacct
    21241 caagctaagc cagaggttcc agatgaatta ttttcccagt gtccaggctg taagcatacc
    21301 atctatcaga aggatctggg aagtgaacgt atctgtccgc actgtagcta tacctttcgt
    21361 atttctgccc aagaacgctt ggctttgacg attgatatgg gaaccttcaa agaattgttt
    21421 acagggattg aaagcaagga tcccttgcat ttccctggtt accaaaagaa actggcatct
    21481 atgcgtgaaa aaacaggtct gcatgaagcc gttgtgacag gaactgctct tattaaaggt
    21541 cagactgtgg ctcttgggat tatggattct aactttatca tggcttctat gggtacggtt
    21601 gtaggtgaaa aaatcactcg tttgtttgag tatgcgactg tcgaaaaatt gccagttgtc
    21661 ctattcacag cctctggtgg agcccgtatg caggaaggaa tcatgagtct catgcagatg
    21721 gctaagatct ctgcggcggt taaacgtcat tcaaatgctg gtctctttta cctgaccatt
    21781 ttgacagatc caacgactgg tggtgtgaca gcttctttcg ctatggaagg cgatatcatt
    21841 ctggctgaac cacagagctt ggttggtttt gctgggcgtc gtgtgattga aaatacggtt
    21901 cgtgaaagct tgcctgagga tttccaaaag gcagaattcc tattagaaca tggctttgtg
    21961 gatgctattg tcaaaagaag agacttacca gatacgattg ctagcctagt cagattgcat
    22021 ggagggagtc ctagatgaat attgcaaaaa tagtcagaga agcgcgtgag cagagtcgct
    22081 tgacaacctt ggactttgcg acaggcattt ttgatgaatt tatccaatta catggtgacc
    22141 gttcttttcg tgatgatggt gcagttgttg gtggtattgg ttggcttgga gaccaagctg
    22201 taacagtggt tggtatccaa aaaggcaaga gtttgcaaga caacctcaaa cggaattttg
    22261 gccaaccaca tccagaaggc taccgaaagg cactgcggtt gatgaaacag gctgagaaat
    22321 ttggccgtcc agttgtgacc tttattaata cagcaggtgc ttatcctggt gtcggagcag
    22381 aagaacgtgg tcaaggggaa gctatcgctc gcaatctcat ggaaatgagt gacctgaaag
    22441 ttcctattat cgccattatt atcggtgaag gtggttcagg cggggctctg gctctagctg
    22501 tcgcggaccg tgtctggatg ctggaaaatt ctatctatgc cattctcagt ccagaaggct
    22561 ttgcttccat tttatggaag gacggtactc gcgccatgga agcagcagaa ctgatgaaaa
    22621 tcacttcgca tgaactgtta gaaatggacg tggtggataa ggtgatttct gaagtaggac
    22681 tttctagtaa agaactgatt aagagtgtca aaaaagaact ccaaacggag ctggctagac
    22741 tttcacaaaa atcgctagaa gagttgctgg aagaacgcta tcaacgattt agaaaatact
    22801 aagaatgact aaatgagact agaaaggtct cattttttat ggaaaaatgc aattcaggtt
    22861 gaaaaattga tagttgagga gatgggctat aaaatagaca aaaaagaaat atactataaa
    22921 tgtgagccta caacaatcta ttttaagaag gtacttgcaa tgacaggtac agaaacattt
    22981 acagttattt caactgagga cttggagcaa acttcaggtg gtcttgctgt ttgggaagat
    23041 ggatatagta gatggttata ttatagagaa tttgctccct atatgaggca agggcactta
    23101 attcttatat agatgcttgg aagtacggct tccgaacagg gtaatctttc aaaaaagtac
    23161 gatgagaaac acaaagaaac taagacaatt tggaatattt ttgcttatta ttttactatc
    23221 tacctattta ccacaaacaa ttgaattgta tgtgacgatc attttagggt taggagctga
    23281 tgtctattct cttattttaa caatggtatt agtgggaagt ttccttctat taatttggag
    23341 gttaaaaaag aaaaagagct ggaactggtc atttgtcttc tccttatttg caacttatgt
    23401 ggtttatcag attctcggta atttttgggc acgctatgct catttgatta atcataggaa
    23461 tattcatgat gagtatttta ccgtggttct ttcaaatgga caaccaacct ttttatcgac
    23521 tatcttatct tttgtcctgc ctgtaatcat cggtcctgtt tttgaggaga ctcttgatag
    23581 agggtatttt atgaatacct tctttcccta gtccaagtat tatttagatg tcatcttatc
    23641 aggccttatc tttgggctta gtcatttgat actatctcac agagatccaa ttagtttatt
    23701 atattatagt ctgattggtt tcttttttgc ccttgtttat cgttttaccg acaatctgag
    23761 attaacgatt ctctgccata gtttttttta actttctcaa tcatgcaaaa cctatctgga
    23821 tttttgttta caattatatc tattatcatt tttttagata gtgggaggca aagaaaaagt
    23881 gtttgatatc tatcaaacac tttttctttt tattgttctt ctgttacaaa ctggctgagc
    23941 agtccattga taaaacgggc agatttttga tcggagaagt cctttgcaag ctcgatagct
    24001 tcattaacag caaccagctg aggagtgtca aatgaagtga tttcaaagac tcccaagcga
    24061 aggaggtttc tctccacgag cgttaagcgt tcaatggtcc aacctgcttt tagatgctga
    24121 gtgatttgct tatctagttc ttccttttta gcttgaacac cagaaacgag gtctatcaaa
    24181 aaggctggaa gttgtacatc cgtatcttca cgatcatgag tataggcgaa acgacaagca
    24241 gtttcgacat ccgtaccgaa ctcaaggctc atgagagctt gaaaagcgca tttacggagt
    24301 tggcgtctag attctaatag tggactagtc attgaggaag tcctcgtcaa atagatcttt
    24361 caattctggt tttggtgttt tatctgggac gatacctgca acgtgaatat tgatagcagc
    24421 gagttctaca tcagccatat tacggacggc atctttgaca gctttctgga tagcaaccgc
    24481 taccttagga acttttactc cgtactcaag gtagagatag atatctgctg tgagttcttc
    24541 gtccacgttt ttaagataaa cgccacggcc gagtgaaagt tttgaaaggg tatcagacac
    24601 tgatctgttt gaaaaagagt gaacaccctc tacctttgca gtagcgatag caatgatttt
    24661 ttcaagtaca cgtggggcga taacgatttc gccaagttgt tcttcaattc ccatagaatg
    24721 acctctttct agagattagg cacgagaaac gtaagttcct tctgcagtgt tgataacgag
    24781 tttttgtcct gcttcgatga agtctggaac gtttacgaca agtccagttt ccatcgttgc
    24841 tggtttacca gaacctgtaa cagtagcacc tttgatagat ggttgagttt cagcaactgt
    24901 caactcaaca gtagtaggaa cggtgacacc gatcacttca gttccgtaga attggatttt
    24961 cacatcagag ttttcaagga tgtaaagcaa ttcgttttca acattgacta cagggatttc
    25021 gtactggtcg taagtttctg tattcatgaa gtatgctgtg tcatccattt tgtacaagta
    25081 ttgagctggg acagtctcga taatagcttg ttcaaatttt tcctctggac ggtagcttgt
    25141 gtcaaatgta gaaccagtac ggacatcacg caatttcata cgcatgatcg tgtttccttt
    25201 acctggtttg tggtgactag cttccaaaac gcgaatcaat ttgccgtcag ctgtttcaaa
    25261 ggtcatacca gcttttaatt tacttgcttc aatcatgttt ttacctcttt aataaatatt
    25321 attactcttc attttaccat aaaatactct ggaaataaag ccaaaatagt tattgggagt
    25381 tggtagagaa gaaaaaacca accttagggc tggttttgtt ctgtctagtt ttctttcaac
    25441 ttcgccaatt cctgtgcaag tagtttaagg gcaacttgtg ggttggcttg gcctttggtt
    25501 gccttcataa ggaatcctgt aaaggctttg tcggcgttac gtttgcctga cttgaagtcg
    25561 gcaacagcag cttcgttatc ggcaaagact tggtggatga ttgggatcaa gatagctgga
    25621 tctgaaattt gaaccatacc tgctttttcc acgtattcac gcgcgccacc gccattttta
    25681 gctagatgga caaagacttt cttggcaatc ttagatgaaa tagtaccgtc ttcgatgatg
    25741 gtaatcattt caaccaagtt ttctggtgtc aattcgattt gttccagtgt tttaccttca
    25801 gcattcaaga actgagcgac ttccccttgg agccagttag agacttgttt ggcatcacca
    25861 cctagggcaa cagctttttc aaagaagtca gaagtgactt tattagcagt caactgacta
    25921 gcatcgtagt ctgataaacc aaggtcagat acataacgcg cacgacgttc ttttggaaac
    25981 tctggcaact cagtccgcat ttcctcaatc cactcgtcag aaatttcaaa gaggggtagg
    26041 tctggttctg ggaagtagcg gtagtctgca gccccttcct tgacacgcat gagaatggtt
    26101 gctttattcg cttcatcgta acggcgtgtt tcttggcgga tttgaccacc tgagcgaaga
    26161 atttcagcct ggcgttggac ttcgtattca agacctttac gaacgtttga gaaggagttg
    26221 aggttcttca attcagtctt ggtaccgaat ttttcttgac cataagggcg aagggagatg
    26281 ttggcatcca cacgcataga accttcttcc atcttaacgt cagaaatgcc agcgtactgg
    26341 ataacttcct tgagggctgt cagataagca taggcctcct ctggcgaacg catatctgcc
    26401 tcagatacaa tctcaatcaa gggaacccct tggcggttga ggtcaacata agagtagcca
    26461 tctgtaccat gggtgttttt accagcgtct tcctctaggt gggcacgttc gataccgatt
    26521 ttcttggtcg taccgtcttc tagtttgact tcaatccagc cattatatcc gattggttca
    26581 tcaaattgag aaatttggta ggctttgggg ttatcaggat agaagtagtt cttgcggtca
    26641 aagtgcatct ttttgtggat gtccatgttg agggcaagag cagccttgat accggcatca
    26701 acaacccctt tattgagaac tggtagaact cctgggaaag accagtcaat cacgttagtg
    26761 ttggcatttt ggtcatttcc aaagtgggca gaagtaggtg agaagatttt tgaattggtg
    26821 ttgagctcta cgtggacttc aagtccgatg actgtttcaa agttcattag ttgtcacctc
    26881 caaaaatcac gggttgttgt ttgtggtagt ctgttgttgc ttcaaaagca gcagcagctt
    26941 ggtaaatggt ttcctcagag tacttgggac caatcaattg gagtccgaca ggtagacctt
    27001 gagagaatcc agcaggaatc gaaattccag gcagtcctgc caagtttaca ggtatggtca
    27061 ataggtcggc taagtacatg gcaactgggt catggttgag agaatccaag tcataggcaa
    27121 cacttggagc agttggaccc aaaatcaaat cgtaatccgc gaagactttt tcgaaatctt
    27181 gaatgataag tgtacggacc tgtccagcct tcttatagta ggcatcgtag taacctgatg
    27241 aaagactgaa agtacccagc atgatacggc gcttcacttc ttcaccaaaa ccttggcttc
    27301 ggctgtttac atagatttca tcaaggttgg ttgcatcttc tgcgcgatag ccgtaacgga
    27361 taccgtcaaa gcgttgcaag tttgatgaag cttctgatga agcgatgatg tagtatacgg
    27421 caactccgta tttagagtga ggaaggctga cttcttcgac aatagcaccc aatttttcaa
    27481 agtgtttagc ggcatttaga atggtttcct taacctctgg gttaattcct tcaccgaggt
    27541 attccttagg caaagcgatt ttcataccct tgatgtcttg gccgattttt gaagtaaagt
    27601 cggcgatgcg gacaggagca gaagtagagt ctttagcatc ttcgctggca atagcgttga
    27661 gcaagagggc attttcctta acagtaggag caaaaggtcc aatctggtct aatgagctac
    27721 caaaggcaat gagaccgaaa cgtgaaactg ttccgtaggt tggtttgaga ccaacgattc
    27781 cgttgaaggc agcaggttgg cggatggaac caccagtatc agaaccaagt gacaagcgaa
    27841 cttgtcctga ggctacagct gcggcagaac cacttgatga cccaccagga accttgctgt
    27901 ggtcccaagc gtttttagtt gctccgtagt gtgaagtttc acctgaacca cccatagcaa
    27961 attcgtccat gttggtcttt ccaacgacaa tcatgccctt ggtttttgca ttggcaacag
    28021 ctgtcgcatc aaagattggc tcatagttgt agagcatttt tgaggcagca gttgtgagaa
    28081 taccgtctgt agagatgtta tccttaacag caagtggaat tcctgaaagg acattgtcag
    28141 catcaattcc agcttcatca atggctttag cttgaacaag agcttgctcc tcagcgatgg
    28201 tgacaaatga attgagggct tcctcacgag acttgatatt ttcaagtgtt gcttgggtca
    28261 attctgttgc agaaatttcc ttagagacaa ggagattgtg caactcttca atagttttat
    28321 tgttaaaagt cattaggcat ctcctccatc gtctaggata gctggcacct tgatatagta
    28381 gttgtctttt tcaggtacgt ttttaaacaa gcgatcacgg tctgttcctt cttcggccac
    28441 atcagggcgg agtacagtct tgcggtcagc catagtcgta gtaggtgcga caccagttgt
    28501 gtcaacttcg cccagcaatt caaccatgtc aacaatctta gacaaggtgg tcgcaaaggc
    28561 agcagtttct ttttcagaga atcttaattt tgaaagattg gcaacgtgtg ttacctcttc
    28621 ttgcgtaatt ttcatcttgc atcctttcgt gaaatgatga tttttagtct gttctatttt
    28681 accatatttt cctataaata agggaggggg aagtgaaaag aaatagaaat tgaaaaaaat
    28741 gctaaaatcc gatataatgg agtgttgaaa ggaatggtaa aatccaatgt aaaaatcatt
    28801 ctcagctatt gaaacaagaa aaatagagaa tcaaagaaag agaacttatg aatattcaag
    28861 aagaaattaa gaaacgtcgt acctttgcca ttatctccca cccggacgcg gggaaaacaa
    28921 ccatcactga gcagttactc tactttgggg gtgagattcg tgaggctggt acggtaaaag
    28981 ggaagaaaac agggactttt gctaaatctg actggatgga tatcgagaag caacgtggga
    29041 tttctgttac ttcatctgtt atgcaatttg actacgacgg caagcgcgtg aatatcttag
    29101 acacgccagg gcacgaggac ttctcagaag atacctatcg taccttgatg gcggtggatg
    29161 ctgcggtcat ggtcgtggac tctgccaagg ggatcgaggc tcaaaccaag aaattgtttg
    29221 aggttgtgaa acatcgtggc attccagtct ttacctttat gaacaagctg gaccgtgacg
    29281 gtcgtgagcc tttggatctc ttgcaagaat tggaagaaat cttgggcatt gctagctacc
    29341 ctatgaactg gcctatcggg atggggaaag cctttgaggg cttgtatgac ctctataacc
    29401 aacgtttaga gctttacaaa ggggatgagc gttttgccag tctagaagac ggggacaaac
    29461 tctttggtag caatcctttc tacgagcaag tcaaggatga cattgagctt ttaaatgaag
    29521 ccgggaatga gttttcagag gaagctattc tggctggaga attgacgcct gtctttttcg
    29581 gttcagccct gacaaacttt ggtgtgcaga ccttccttga aaccttcctc aagtttgctc
    29641 cagaaccaca tggtcacaag aaaacagacg gtgaaattgt ggatccttat gacaaggatt
    29701 tctcaggatt tgtctttaaa atccaagcca acatggaccc tcgtcaccgt gaccgtattg
    29761 cctttgtccg tatcgtatct ggcgaatttg agcgcggcat gagtgtcaat ctccctcgta
    29821 ctggtaaggg ggctaaactg tctaatgtta ctcagtttat ggcggaaagt cgtgagaatg
    29881 tgatcaatgc cgtggcaggt gatattatcg gggtttacga taccggtact tatcaagttg
    29941 gggatacctt gacagttggt aaaaacaagt ttgaatttga accactgcca acctttactc
    30001 ctgaaatttt catgaaagtt tctgctaaga atgttatgaa gcaaaaatcc ttccacaagg
    30061 ggattgagca attggtgcaa gaaggagccg ttcagcttta taagaattac caaacaggtg
    30121 agtacatgct aggcgctgta ggtcaactcc agtttgaagt ctttaaacac cgcatggaag
    30181 gcgagtacaa tgcagaagta gttatgaacc caatgggtaa aaagaccgtt cgttggatca
    30241 agcctgagga cttggatgaa cggatgtcgt caagtcgcaa tatcttagcc aaagaccgtt
    30301 ttgaccaacc agtctttctc tttgaaaatg actttgccct ccgctggttt gcggacaagt
    30361 atccagacgt agagttagag gagaagatgt gactcagtac caacaattgg aactaaagtt
    30421 ccaaattgtt ggacgctagt cgctatttgg cgaactagct aactgcctca ctaactacgt
    30481 ttggcaaata gaatcgattt gccaaactcc gtgtcgtaat ataataatcg gtaaccgcta
    30541 tggcggctta cctaaacgct tcactaggaa aaatccacga atggtattga tttgtggatt
    30601 tttccgtcgt agcagaaagt aggtagccta gctaactgcc ttactaacta cgtttggcaa
    30661 atgaaatcga tttgtaaaac tccgtgtcgt aatgtaaaaa ttgtaaaccg taatgggact
    30721 tcatctaaaa tctccactgg tatggtttta cgaacgaata ggcgaaaata atggttacgt
    30781 agaaacatat gatgggaata atatgatggg aataaaaaga aataagataa agagtgaaag
    30841 aagagaaaaa agcgattgtc ttaggtgcag ataatgctta tatggataaa gttgaaacaa
    30901 caataaaatc tctttgtgtt catcattata atcttaagtt ttatgttttt aatgatgacc
    30961 ttccgcgaga atggttccaa ttgatggaaa aaagactaga gactttgaat agtgagattg
    31021 tgaatgttca gattgacagt agtattctga aaggttatcg tctccccttt gagggacttt
    31081 catatgcggc tttttttagg tattttattc caaagtatgt aagcgagtct cgtgtactgt
    31141 atttggattc agatattgtt gtaagaaaat cgatagatga gctgtgggat ttggacttga
    31201 cggctatacc cttagctgca gttagagatg atttttatac acataatttt aattctggtg
    31261 ttctgttaat caacaatggc atgtggagag cagaaaatat cacgcaagac ctgatttaag
    31321 acacaaataa aaaatcttcc aattcccttg caaaaaacaa gaaagtgtga taacatagta
    31381 cggtatgtgg tgctagcaca tccgctatat tagatctaat aggaggaaaa cacaatggct
    31441 aaagtatgtt actttacagg tcgtaagact gtatcaggaa acaaccgttc acacgcgatg
    31501 aaccaaacaa aacgtgccgt aaaaccaaac cttcaaaaag ttactgttct tatcgatggt
    31561 aaacctaaaa aagtttgggc ttcagctcgt gctttgaaat caggtaaagt tgaacgcgtt
    31621 taataataaa agcaaaggag accttagggt cttttttctt ttattgtggg tataacatcg
    31681 tttgaaaaat agagtaaata tccgctgata cattattctg cttttacact tgggctgaaa
    31741 tatgataaaa tagagtatca actagttgag gtaaaaagga tgactgtaaa aattaataca
    31801 aaagatggtc aaatcgaact aacagatgaa gtgattgcaa ccgtcgtagg tggagcagca
    31861 actgagattt ttggtgtggt cggtatggct agtaaaaatg ccctcaaaga taatttccaa
    31921 gccctgctag gtaaagaaaa ttattctaaa ggtgtcgtcg taaaggcagc cgaagatggc
    31981 agtattgcag ttgatgtata tactgtgtta agctacggaa caaagattag tgaagtgtca
    32041 aaaaacattc aagagcgtgt tgcctttagc ttggaaaacc agcttggaat tactgctcag
    32101 actgtaaatg tctacattca aaatatcaaa gttgtaggag aataaccgtg tcaaaaatta
    32161 ctactagctt atttcaagaa atggtgcagg ctgcatcaac tcgcttgaat aagcaagctg
    32221 aatatgtcaa ttcattaaac gtctttccag ttccagatgg agatactggg acaaatatgg
    32281 gaatgaccat tgaaaatggt gctaaagaag ttgcagacaa gccagcttct acagttggag
    32341 aagtagcgag cattcttgcc aaagggcttt tgatgggtgc gcgtgggaac tcaggagtga
    32401 ttacgtctca gcttttccgt ggattttccc aagctatcaa ggataaagac gagttaacag
    32461 gtcaagactt ggctctggcc ttccaatcag gtgtggaagt tgcctataag gcagtgatga
    32521 aacctgttga aggaacgatt ttgacagttt ctcgtggagc tgctatcggt gctaagaaaa
    32581 aagctgagca aacagatgac actgttgaag tcatgagcgc agccttggaa ggtgctaaaa
    32641 cagctctagc taaaacacca gacatgcttc cagtattgaa agaagttggc gttgtggact
    32701 caggtggtca aggattggtc ttcatctacg aaggtttcct ttcagccctt actggcgaat
    32761 atattgcatc tgaggacttt gtagcgactc ctgccaacat gagtgagatg atcaatgtag
    32821 agcatcataa gtctgtagct ggtcacgtag cgactgagga catcacgttt ggttactgta
    32881 ctgaaatcat ggtagctctt aagcaaggtc caacctatgc taaagatttt gactacgatg
    32941 aattccgtaa ctacttggat gagcttggag attctctcct cgttgttaac gatgatgaaa
    33001 ttgtcaaagt ccatgttcat acagaagatc caggacttgt tatgcaagaa ggtctcaaat
    33061 atggtagctt ggtcaaggta aaagttgaca atatgcgtaa ccaacacgaa gcacaggttg
    33121 agaaagaagc tactcaagtt atcaagtcgg ctgaagaaaa agagtatgct ttgattgctg
    33181 tggtggctgg taaaggtcta gcagatatct tctgttctca aggcgtggat tatgttatcg
    33241 aaggcggtca aaccatgaac ccttcaacag aagactttat caaggctgtt gaacaggtta
    33301 atgcccgtaa catcatcttc ttgccaaaca acaagaacat cttcatggca gctcaatctg
    33361 cggcagaagt tttggagcaa ccagcggtag tggtagaggc tcgcactctt cctcaaggta
    33421 tgacaagtct tcttgccttt gatccaagca agtccattga agaaaaccaa gagcgtatga
    33481 cagctgctct tagcgatgtc gttagcggaa gcgtcacaac agccgtgcgt gatacaacga
    33541 tcgatggctt agaaatccat gaaaacgata atttaggtat ggtggatgga aaaattcttg
    33601 tgtcaaaccc tgatatgcac caaacattga ctgaaacctt gaaacatatg ttggatgaag
    33661 acagtgaaat cgtaaccttc tatgtcggtg aagacggaag cgaagaactt gccaatgaaa
    33721 tcgctcaaga aatcgtagaa gaattcgaag acgttgaagt cgagattcac caaggtcaac
    33781 aacctgttta cccataccta tttagtgtgg aataagatta atgaaggact gagaaatcag
    33841 ttcttttttc tttttagtaa atgaaatcgg tatcttttta ataaaaacaa aaataacatt
    33901 cataaataaa cgttaaaata gaaaattcag aaaatttctt cttttatctt gaaaaatttt
    33961 gaaaaaatgg tatgatagta acaagttatt tttaagagga aagaaagggg aataatggag
    34021 aaaatcagtt tagaatctcc taagacgggg tcggacctag ttttggaaac acttcgtgat
    34081 ttaggagttg ataccatctt tggttatcct ggtggtgcgg ttttgccttt ttatgatgcg
    34141 atatataatt ttaaaggcat tcgccacatt ctagggcgcc atgagcaagg ttgtttgcat
    34201 gaagctgaag gctatgccaa atcaactgga aagttgggtg ttgccgtcgt cactagtgga
    34261 ccaggagcaa caaatgccat tacagggatt gcggatgcta tgagcgatag cgttcccctt
    34321 ttggtcttta caggtcaggt ggcgcgagca gggattggga aggatgcctt tcaggaggca
    34381 gacatcgtag gaattaccat gccaatcact aagtacaatt accaagttcg tgagacagct
    34441 gatattccgc gtatcattac ggaagctgtc catatcgcaa ctacaggccg tccagggcca
    34501 gttgtaattg acctaccaaa agacatatct gctttagaaa cagacttcat ttattcacca
    34561 gaagtgaatt taccaagtta tcagccgact cttgagccga atgatatgca aatcaagaaa
    34621 atcttgaagc aattgtccaa ggctaaaaag ccagtcttgt tagctggtgg tggaattagt
    34681 tatgctgagg ctgctacgga actaaatgaa tttgcagaac gctatcaaat tccagtggta
    34741 accagtcttt tgggacaagg aacgattgca acgagtcacc cactctttct tggaatggga
    34801 ggcatgcacg ggtcattcgc agcaaatatt gccatgacgg aagcggactt tatgattagt
    34861 attggttctc gtttcgatga ccgtttgacg gggaatccta agactttcgc taagaatgct
    34921 aaggttgccc acattgatat tgacccagct gagattggca agattatcag tgcagacatt
    34981 cctgtagttg gagatgctaa gaaggccttg caaatgttgc tagcagaacc aacagttcac
    35041 aacaatactg aaaagtggat tgagaaagtc actaaagaca agaatcgtgt tcgttcgtat
    35101 gacaagaaag agcgtgtggt tcaaccgcaa gcagttattg aacgaattgg tgaattgacg
    35161 aatggagatg ccattgtggt aacagacgtt ggtcaacacc aaatgtggac agctcagtat
    35221 tatccctacc aaaatgaacg tcagttagtg acttcaggtg gtttgggaac aatgggcttt
    35281 ggaattccag cagcaatcgg tgctaaaatt gctaacccag ataaggaagt agtcttgttt
    35341 gttggggatg gtggtttcca aatgaccaac caggagttgg ctattttgaa tatttacaag
    35401 gtgccaatca aggtggttat gctgaacaat cattcactcg gaatggttcg ccagtggcag
    35461 gaatccttct atgaaggcag aacatcagag tcggtctttg atacccttcc tgatttccaa
    35521 ttgatggcgc aggcttatgg tattaaaaac tataagtttg acaatcctga gaccttggct
    35581 caagaccttg aagtcatcac tgaggatgtt cctatgctaa ttgaggtaga tatttctcgt
    35641 aaggaacagg tgttaccaat ggtaccggct ggtaagagta atcatgagat gttgggggtg
    35701 cagttccatg cgtagaatgt taacagcaaa actacaaaat cgttcaggag tcctcaatcg
    35761 ctttacaggt gtcctatctc gtcgtcaggt taatattgaa agcatctctg ttggagcaac
    35821 agaagatccg aatgtatcgc gtatcactat tattattgat gttgcttctc atgatgaagt
    35881 ggagcaaatc atcaaacagc tcaatcgtca gattgatgtg attcgcattc gagatattac
    35941 agacaagcct catttggagc gcgaggtgat tttggttaag atgtcagcgc cagctgagaa
    36001 gagagctgag attttagcga ttattcaacc tttccgtgca acagtggtag acgtagcgcc
    36061 aagctcgatt accattcaga tgacgggaaa tgcagaaaag agcgaagccc tattgcgagt
    36121 cattcgccca tacggtattc gcaatattgc tcgaacgggt gcaactggat ttacccgcga
    36181 ttaaaaatcc aacttaaatt tgttaaacca gcttaaaagg caataaataa tagaaaagag
    36241 agaaaagcta tgacagttca aatggaatat gaaaaagatg ttaaagtagc agcacttgac
    36301 ggtaaaaaaa tcgccgttat cggttatggt tcacaagggc atgcgcatgc tcaaaacttg
    36361 cgtgattcag gtcgtgacgt tattatcggt gtacgtccag gtaaatcttt tgataaagca
    36421 aaagaagatg gatttgatac ttacacagta acagaagcta ctaagttggc tgatgttatc
    36481 atgatcttgg cgccagacga aattcaacaa gaattgtacg aagcagaaat cgctccaaac
    36541 ttggaagctg gaaacgcagt tggatttgcc catggtttca acatccactt tgaatttatc
    36601 aaagttcctg cggatgtaga tgtcttcatg tgtgctccta aaggaccagg acacttggta
    36661 cgtcgtactt acgaagaagg atttggtgtt ccagctcttt atgcagtata ccaagatgca
    36721 acaggaaatg ctaaaaacat tgctatggac tggtgtaaag gtgttggagc ggctcgtgta
    36781 ggtcttcttg aaacaactta caaagaagaa actgaagaag atttgtttgg tgaacaagct
    36841 gtactttgtg gtggtttgac tgcccttatc gaagcaggtt tcgaagtctt gacagaagca
    36901 ggttacgctc cagaattggc ttactttgaa gttcttcacg aaatgaaatt gatcgttgac
    36961 ttgatctacg aaggtggatt caagaaaatg cgtcaatcta tttcaaacac tgctgaatac
    37021 ggtgactatg tatcaggtcc acgtgtaatc actgaacaag ttaaagaaaa tatgaaggct
    37081 gtcttggcag acatccaaaa tggtaaattt gcaaatgact ttgtaaatga ctataaagct
    37141 ggacgtccaa aattgactgc ttaccgtgaa caagcagcta accttgaaat tgaaaaagtt
    37201 ggtgcagaat tgcgtaaagc aatgccattc gttggtaaaa acgacgatga tgcattcaaa
    37261 atctataact aattagaaat atatagcgct ggagatgatt ttatgaaaaa gattatgaga
    37321 aaaattgcat cgttattatt ggttctagtt gtataatgta attacaccgt cggtaatagt
    37381 gctagcagac caaaataaag cagattggtc gtatgatgaa aatactgtaa ttaacattta
    37441 tgatgatgct aattttgaag atggtaggtt gcatatgacc tttgaacaat tcttcaaatt
    37501 ggcacaaata gctagagaag aaggtcttga aattcattct tcgtttgaga gagctggtgc
    37561 gactaaatct gctcgttata tagcgaaatg gattttgaga aataaaaaac attaacaaat
    37621 atagttggta aatcattagg acctaaatca gctgttagat tcggagaagc tttatcctat
    37681 attgaaggtc ctcttcgcag aataaatgag acgatagatg gcggtttata tcaaatagag
    37741 caaattattg catctggatt gaaagaatcg ggtttaaatg actggactgc gaaaacttta
    37801 gcttcagcta ttcgtgggat attagatgta cttatttagg ggttgaaatc atatgaatat
    37861 taccaatttg ttttctatca agacaggatg tgatgaaact gataggcaac tgcaaaaact
    37921 attttttcag ttggatttac aattgggaga attgacagat caactaagaa aattagattc
    37981 taattttgtt cctcgtagtc aatttgtaga cacgttggat ttgaatgatg tagaatataa
    38041 agaaatttta aactatttta tcttccatcg taatgatagt gaagaaagtt tggtagaatg
    38101 gttatatgat tggatttcca caaatcgtta tgagcttcct aaagagtttt cgattcgtat
    38161 ggctcataaa taccatgaaa gtgttactga agttttcgga gatgaataac taaaaaacag
    38221 tcattagtga ctgtttttta tagaaaaaga ggttttatat gttaagttca aaagatataa
    38281 tcaaggctca caagatcttg aacggtgtgg ttgtgaatac tccactggat tacgatcatt
    38341 atttatcgga gaagtatggt gctaagattt atttgaaaaa agaaaatgcc cagcgtgttc
    38401 gctcctttaa aattcgtggt gcctattatg ccatttccca gctcagcaag gaagaacgtg
    38461 aacgtggggt agtctgcgct tctgcgggaa atcatgcgca gggagtagcc tatacttgta
    38521 atgaaatgaa aattcctgct actatcttta tgcctattac tacgccacaa caaaagattg
    38581 gtcaggttcg cttttttggt ggggattttg taactattaa actagttgga gatacctttg
    38641 atgcctcagc caaagcagct caagaattta cagtctctga aaatcgtacc tttattgatc
    38701 cttttgatga tgctcatgtt caagcaggtc aaggaacagt tgcttatgag attttagaag
    38761 aagctcgaaa agaatcgatt gattttgatg ctgtcttggt tcctgttggt ggtggcggtc
    38821 tcattgccgg ggtttctacc tatatcaagg aaacaagtcc agagattgag gttatcggag
    38881 tagaggcgaa tggagcgcgt tccatgaaag ctgcctttga ggctggaggt ccagtcaaac
    38941 tcaaggaaat tgataaattt gcggatggga ttgctgtgca aaaggtaggt cagttgacct
    39001 atgaagcaac tcgtcaacat attaaaactt tggtaggtgt cgatgaggga ttgatttctg
    39061 aaaccttgat tgacctttac tctaagcaag ggatagtcgc agaacctgct ggagcggcta
    39121 gtatcgcctc tttagaggtt ttagctgaat atattaaggg gaaaaccatt tgttgtatca
    39181 tttctggagg aaataatgat atcaaccgta tgccagaaat ggaagagcgt gccttgattt
    39241 atgatggtat caaacattac tttgtggtca atttcccaca acgtccagga gctttgcgtg
    39301 agtttgtaaa tgatatcctg gggccaaatg atgatatcac acgttttgag tatatcaaac
    39361 gagctagcaa gggaacaggc ccagtattaa ttgggatcgc tttagcagat aagcatgatt
    39421 atgcaggttt gattcgtaga atggaaggtt ttgatccagc ttatattaac ttaaatggta
    39481 atgaaacgct ttataatatg cttgtctgag gactaataaa aaaatatcat attatttgta
    39541 caactgatgt atatgtgcta taatgaagat gaagaaaggg gacgatttct atggttttag
    39601 gaaacttatc gtgcaattct tacttctgtt cattatagca cttatgatgt ttggctcaga
    39661 tttccatcct tcattttgat ttcctatcta ttgacaagca tagtcacact gtctttaata
    39721 ctcttcgaaa atctcttcaa accacgttag ctctatctgc aacctcaaaa cagtgttttg
    39781 agcaacttgc ggctagcttc ctagtttgct ctttgatttt cattgagtat aaggtatgat
    39841 ttgatttctt tttgttgaca aatataccat attaaaaaga tatataagta attaactgag
    39901 cttatctgtc ttgtcatctc tattaaggat ggtttagata atcgggtgtc tgcttctagg
    39961 ctagcacctc aatatccaaa ggagtgatga atttgaagga tataaggaat acctatctct
    40021 cagatgattt attgaggaag aaagatagga gtttttgagc tagtgaaggc ttggatttct
    40081 aaaggttaga actatcatct tcagttctta aatcgaagaa ataagctatc ttacggaaat
    40141 agagaagcat tttttaagaa cttgaataat ttcgcacctt aagagggtaa taatacagta
    40201 tttttattag caaatattta tggtgtagag actagtaaaa cctatatatt atcggattca
    40261 aaaaggaagt aagaaaattc tgaagctaaa attctgaact aattattttc gttgaataca
    40321 tgttttttta ttgacaaaag tagtaaaaac gtatacaatt aaatattata aagaagaaaa
    40381 taggagaaaa acatggctaa gtcaaacttt gaaaaagtag aatcagttgt tggctgggtt
    40441 cgtgataaga aaatcacagg ctaccgtatc tctaaagaaa cgaatgcgcg tgaaatgtct
    40501 atcattgctc tggcgcaggg tcgtgcaaaa gtaaaaaata tttcatttga aacagcccta
    40561 ggcctaattg atttctatga aaaaaatcat gaaaaatttg aagattaatc tttggataac
    40621 ggcggattct tgaccttcaa gtagtagaga tagagaatct gccttttcat tttgaggaca
    40681 gcaaaaagac tgcacggttg atgcagcctt ttctttttat ttgagatagc gttgaaggaa
    40741 ctcttttgtt cggtcttctt taggattggt gaagaggtct tctggtttac cttcttcagc
    40801 gatcacgccc ttatccataa agataacacg gtgagagaca tcacgggcaa attccatttc
    40861 atgggttacg acaatcatgg tcaagccttc ctgagccaga tcttgcatga ttttgaggac
    40921 ctctccaacc atttctggat cgagagctga tgttggttca tcaaagagaa tggcgtcagg
    40981 attcattgaa agggcacgag caatggccac acgttgtttt tgaccacctg agagttgttt
    41041 gggtttagct tgccagtagc gttctcccat gccgaccttt tccaggtttt ctttggcaat
    41101 ttcttcagct tctgtgcgtt cgcgtttaag gacagttgtc tgagcgacga ttgtgttttc
    41161 aagaacgttg agattttcaa agaggttaaa ggattggaaa accatcccta acttttcacg
    41221 gtattgcgtg aggtcatagc ctttttcgag gacgttttgt ccatgataaa ggatttgtcc
    41281 atcagttggc gtttcaagta ggttaatgga gcgtaggaag gttgatttcc cacttccaga
    41341 gcttccgatg atagaaataa cttctccctt gtggacagtg agagaaatgt cttttagcac
    41401 ttcgttttgt ccataggatt ttttgaggtg tttaatttca aggattgctt gtgtcattat
    41461 ttcaaatcct ccgtttgcat ttggttagca cctgtagtgt aggtatccat gtccattctg
    41521 cgctcgataa agcgtaggat acgtgttacg gtgaaggtga ggacaaagta aatcacggcg
    41581 atgattgtaa atgtctggaa gtattgatag gtttgtgttg ccacggtatt tcctgagaaa
    41641 taaagttcga caacagagat aacgttcaat acagatgtat ctttgatatt gatgacaaat
    41701 tcattaccag ttgcaggtag gatgttacgg actacctgag gtaggacaat cttacgcatg
    41761 gtctggttat gggtcatacc aagagcagtc gcagcttcaa attgtccctt gtcaactgct
    41821 aggataccac cacggacgat ttcagtcatg taggcaccgg tattgattga aacgatgaag
    41881 atagcagcca gtgtacggtc aaggttgatc ccgaaagctt gggcagttcc atagtagata
    41941 accatcgatt gaacaatcat tggcgtacca cggaaaattt caatgtagac attgagaacc
    42001 cagccgacta gtttttgaag gccataaatg gctttgtttt cagagagagg agcagtacgg
    42061 aagacaccaa tggcaagtcc aataatgaga cctatgatgg ttccgacgat agagattaaa
    42121 agagtgatac cagcaccacg caagagttgt tgccagtttt cagaaagaat tttagcgact
    42181 tggctaaaga aactactgct agtctcttca gttgttgtag cttcggcagg ttgttccttg
    42241 atcatacgat ccatcagggc aacttggtcg tcctttgaaa tggtttcaat gctggcattg
    42301 atttggctaa tacgattgtc atttttacga agcccgatag cgatagctgt atcttcttcc
    42361 ccagttttga aaccaggttc tacttgaatc atcttgaact tagagttcgc agcttcagca
    42421 gtcagtgctt ctggacgttc agaaacataa gcatcaatga caccagcctc aagagcttgt
    42481 cgcatttgag cgaagtctcc catggctgtt tcttttttag cacctgggat ttgtgcaatc
    42541 aagtcgtaga ggtagacccc ttgttgagaa gtgatttttg caccgttaaa gtcatccaaa
    42601 gatttagcac ttgcgtaggc agaatctttt ttgacaagca aaactggttc gctagtgtag
    42661 taactgctcg aaaaggcaat ttcttgtttg cgttctgcag ttggactcat acctgcgata
    42721 atcatgtcaa tcttaccaga agtaagggca gggactagac cttcccactt ggttttaaca
    42781 accaaaggtt ctttacctaa gtccttagcg attttcttgg cgatttgaac atcgtatccg
    42841 ttggcatact gattggtccc atcgattttg acagctccgt tgctatcatc atcctgggtc
    42901 cagttaaagg gagcatatgc tgcttccatc ccgatgcgta aatattcatc ggcttgagca
    42961 acattgacaa gtcctagcat cagcaagaga cttgtgaaaa tagataagta tatttttctc
    43021 atgatttctc ctattctgat ctattaaaaa ataactgtct cctattttat cgaaaaatgc
    43081 gtaatttttc aacataagta agcctttact tacgaaaaaa tgctataatg ataagaaaga
    43141 taaaaagggg gcttagttga tgaaaaaaac ttttttctta ctggtgttag gcttgttttg
    43201 ccttcttcca ctctctgttt ttgccattga tttcaagata aactcttatc aaggggattt
    43261 gtatattcat gcagataata cggcagagtt tagacagaag atagtttacc agtttgagga
    43321 ggactttaag ggacaaatcg tgggacttgg acgtgctggt aagatgccta gcgggtttga
    43381 cattgaccct catccaaagg ttcaggccgc gaaaaacggt gcagaactag cagatgtgac
    43441 tagcgaagta acagaagaag cggatggtta tactgtgaga gtctataatc caggtcagga
    43501 gggcgacata gttgaagttg acctcgtctg gaacttaaaa aatttacttt tcctttatga
    43561 tgatatcgct gaattaaatt ggcaacctct gacagatagt tcagagtcta ttgaaaagtt
    43621 tgaatttcat gtaaggggag acaagggggc tgaaaaactc tttttccata cagggaaact
    43681 ttttagagag ggaacgattg aaaagagtaa ccttgattat actatccgtt tagacaatct
    43741 tccggctaag cgtggagttg agttgcatgc ctattggcct cggaccgatt ttgctagcgc
    43801 tagggatcag ggattgaaag ggaatcgttt agaagagttt aataagatag aagactcgat
    43861 tgttagagaa aaagatcaga gtaaacaact cgttacttgg gtcctccctt cgatactttc
    43921 tatctccttg ttattgagtg tctgcttcta ttttatttat agaagaaaga ccactccttc
    43981 agtcaaatat gccaaaaatc atcgtctcta tgaaccacca atggaattag agcctatggt
    44041 tttatcagaa gcagtctact cgacctcctt ggaggaagtg agtcccttgg tcaagggagc
    44101 tggaaaattc acctttgatc aacttattca agctaccttg ctagatgtga tagaccgtgg
    44161 gaatgtctct atcatttcag aaggagatgc agttggtttg aggctagtaa aagaagatgg
    44221 tttgtcaagc tttgagaaag actgcctaaa tctagctttt tcaggtaaaa aagaagaaac
    44281 tctttccaat ttgtttgcgg attacaaggt atctgatagt ctttatcgta gagccaaagt
    44341 ttctgatgaa aaacggattc aagcaagagg gcttcaactc aaatcttctt ttgaagaggt
    44401 attgaaccag atgcaagaag gagtgagaaa acgagtttcc ttctgggggc tcccagatta
    44461 ttatcgtcct ttaactggtg gggaaaaggc cttgcaagtg ggtatgggtg ccttgactat
    44521 cctgccccta tttatcggat ttggtttgtt cttgtacagt ttagacgttc atggctatct
    44581 ttacctccct ttgccaatac ttggttttct agggttagtt ttgtctgttt tctattattg
    44641 gaagcttcga ctagataatc gtgatggtgt tctaaatgaa gcgggagctg aggtctacta
    44701 tctctggacc agttttgaaa atatgttgcg tgagattgca cgattggatc aggctgaact
    44761 ggaaagtatt gtggtctgga atcgcctctt ggtctatgcg accttatttg gctatgcgga
    44821 caaggttagt catttgatga aggttcatca gattcaagtg gaaaatccag atatcaatct
    44881 ctatgtagct tatggctggc acagtatgtt ttatcattca acagcacaaa tgagccatta
    44941 tgctagtgtc gcaaatacag caagcaccta ctctgtatct tctggaagtg gaagttctgg
    45001 tggtggcttc tctggaggcg gaggtggcgg cagtatcggt gccttttaaa gagagctacc
    45061 atagactgaa aaagtatgat ataatggaag atagaaaaaa gacaaactat aagaaaagtc
    45121 aatagtttta tctaaactat ttcttatttc aatttgatga tttggcgatg attttagagc
    45181 acggcaaaaa gctattgaaa aagtccattt tttcaaaggt aatcctgtgt taatttcaga
    45241 aattatatca ctttttgttc gtcaaatggc agctcttttt taggatataa aacagggttc
    45301 ggataagttt ttttgcaagg tggatgatgg ctacattgta atgttttcct t
//
LOCUS       DAVKLX010000010        43061 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKLX010000010 DAVKLX010000000
VERSION     DAVKLX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.010"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..183
                     /locus_tag="VW325_000638"
     CDS             <1..183
                     /locus_tag="VW325_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9734079.1"
                     /translation="KIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFI
                     ALNIKKERTNFVLSRA"
     gene            complement(549..1775)
                     /locus_tag="VW325_000639"
     CDS             complement(549..1775)
                     /locus_tag="VW325_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9734080.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(1818..2510)
                     /locus_tag="VW325_000640"
     CDS             complement(1818..2510)
                     /locus_tag="VW325_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9734081.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(2683..4434)
                     /locus_tag="VW325_000641"
     CDS             complement(2683..4434)
                     /locus_tag="VW325_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734082.1"
                     /translation="MRNKKEQWDVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAITVLLAYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNIYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQTEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLYLG
                     ITAGTMYAFIQYINRLFDPLIKVTQNFSTLQTSMVSAGRVFALIDERTYEPLQNDGQA
                     KIQEGNIRFEHVCFSYDGKHPILDDISFSVKKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGLVLLDDVDIRNYSQEELRKNIGLVLQDPFLYHGTIKSNISMYQEISDEQVKDAA
                     TFVDAASFIQDLPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(4424..6169)
                     /locus_tag="VW325_000642"
     CDS             complement(4424..6169)
                     /locus_tag="VW325_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9734083.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQVLLLALFYLLLAAFGMYYLRYVWRMYILGTSYRLGQIMRSRLFEHFTK
                     MSPAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNITQRGKVSYQRIEELLSQESPVQDPEFPLDGIENG
                     RLQYAIDSFAFENEETLTNVHFSLEKGQTLGLVGQTGSGKTSLIKLLLREYDVDRGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNSKLPLSAVEEATKLAQV
                     YQDIVAMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKEMRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            6215..6292
                     /locus_tag="VW325_000643"
                     /pseudo
     CDS             6215..6292
                     /locus_tag="VW325_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(6467..7102)
                     /locus_tag="VW325_000644"
     CDS             complement(6467..7102)
                     /locus_tag="VW325_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9734084.1"
                     /translation="MTLNYMEILIKLALGLFSLVFVINVTGKGNLAPNSATDQIQNYV
                     LGGIIGGVIYNSSISILQYAVILMMWTILVLTLKWLNNNVRFVKRLIDGKPTLLIKNG
                     QIDPEACRSVGLSAADVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKYPVV
                     TDGVIQVDVLESIGRSEEWLLDNLSKQGHENVANIFIAEYDKGAVTVVTYE"
     gene            complement(7099..7554)
                     /locus_tag="VW325_000645"
     CDS             complement(7099..7554)
                     /locus_tag="VW325_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002281066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 domain-containing protein"
                     /protein_id="HEU9734085.1"
                     /translation="MKFYSYDYVLSQISQQNGIMIGFGIVLLAITGFFAFKAYRDKKG
                     TKFRELVMILALTLVAMLLVTISKYQTNQASNNQFQTSLHFIEVVSKDLGVDKSEVYV
                     NTSAATDGALVKVGSNVYRAMNGSQPDKYLLEKIELHQTDAIELVEVNK"
     gene            7688..8515
                     /gene="xth"
                     /locus_tag="VW325_000646"
     CDS             7688..8515
                     /gene="xth"
                     /locus_tag="VW325_000646"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9734086.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHLEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTVTFPEI
                     GAPSTMDLEGRIITLEFDKFFVTQVYTPNAGDGLKRLEERQVWDAKYAAYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRLADKVTKSDMIDSGARQDHTPIVLEIEL"
     gene            8527..8913
                     /locus_tag="VW325_000647"
     CDS             8527..8913
                     /locus_tag="VW325_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9734087.1"
                     /translation="MDYQAVIPEFVVSDIEKSRHFYCDLLGFTIEYERPEENFLFLSL
                     EDCQLMLEEGTKDELVDLTYPFGRGVNISFGIVDVPQLHQKLLEADYPIHRPLTKREF
                     RVGDSFIYPHEFAVLDPDGYFLRFSE"
     regulatory      9138..9233
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9363..9944
                     /locus_tag="VW325_000648"
     CDS             9363..9944
                     /locus_tag="VW325_000648"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9734088.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFASAGITKVVTIEASGIAPAIFTAEALNVPMIFAKKAKNITMNEGILTAEVYSFTKQ
                     VTSTVSIARKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVEAIGIVIEKSFQE
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            9944..11206
                     /locus_tag="VW325_000649"
     CDS             9944..11206
                     /locus_tag="VW325_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9734089.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSTEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVVIILLINIFTKGFIKSISILIGLVVGTAIAASMGLVDFSPVAAAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKTRLPIYYAAGFLVLLGLLPKFGALAQIIPSP
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11253..11420)
                     /locus_tag="VW325_000650"
     CDS             complement(11253..11420)
                     /locus_tag="VW325_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnD/PcfM family protein"
                     /protein_id="HEU9734090.1"
                     /translation="MKKYTVEITETLTRLVSIEAENPDEAERLVREKYKSCEIVLDAD
                     DFQDDETSIYE"
     gene            complement(11417..11713)
                     /locus_tag="VW325_000651"
     CDS             complement(11417..11713)
                     /locus_tag="VW325_000651"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734091.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESNYENLCVMVYTLSDPDC
                     VRWLSENMEFGDEKQLSLLNKKYSWEYGDELPEWLESPKHRLLLISELLERNLR"
     gene            complement(11710..12474)
                     /locus_tag="VW325_000652"
     CDS             complement(11710..12474)
                     /locus_tag="VW325_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9734092.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNYGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVKNG
                     QVRDPEKVTKEFKQSLFLRKNSLSVRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(12655..13059)
                     /locus_tag="VW325_000653"
     CDS             complement(12655..13059)
                     /locus_tag="VW325_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9734093.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(13129..14610)
                     /locus_tag="VW325_000654"
     CDS             complement(13129..14610)
                     /locus_tag="VW325_000654"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9734094.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGISLD"
     gene            complement(14629..15807)
                     /locus_tag="VW325_000655"
     CDS             complement(14629..15807)
                     /locus_tag="VW325_000655"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9734095.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            15971..16978
                     /gene="galR"
                     /locus_tag="VW325_000656"
     CDS             15971..16978
                     /gene="galR"
                     /locus_tag="VW325_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9734096.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(17024..18016)
                     /locus_tag="VW325_000657"
     CDS             complement(17024..18016)
                     /locus_tag="VW325_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9734097.1"
                     /translation="MDLRPQIIEADDVIIRVVRACVCGSDLWRYRNPETKAGHKNSGH
                     EAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGSCDNHIGNNLGGDFQA
                     EYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHAARVANVQKGDKVVVI
                     GDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVVAERGQEGITKVREIL
                     GGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRALGSTFMQNISVAGGAA
                     SATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDERKTIKSMIVIE"
     gene            complement(18051..18404)
                     /locus_tag="VW325_000658"
     CDS             complement(18051..18404)
                     /locus_tag="VW325_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9734098.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(18648..19538)
                     /locus_tag="VW325_000659"
     CDS             complement(18648..19538)
                     /locus_tag="VW325_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9734099.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGAVVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLIISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSDLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            19671..20207
                     /locus_tag="VW325_000660"
     CDS             19671..20207
                     /locus_tag="VW325_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9734100.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKDYETTTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHIASLLFS
                     KNDYFLRQLQRELEHHVYPMLAADLKQTHPSLPTSYLQHFVVTNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(20374..21180)
                     /locus_tag="VW325_000661"
     CDS             complement(20374..21180)
                     /locus_tag="VW325_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9734101.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(21525..23045)
                     /locus_tag="VW325_000662"
     CDS             complement(21525..23045)
                     /locus_tag="VW325_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9734102.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVSKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(23038..23766)
                     /locus_tag="VW325_000663"
     CDS             complement(23038..23766)
                     /locus_tag="VW325_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734103.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(23781..24545)
                     /locus_tag="VW325_000664"
     CDS             complement(23781..24545)
                     /locus_tag="VW325_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9734104.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(24549..24992)
                     /locus_tag="VW325_000665"
     CDS             complement(24549..24992)
                     /locus_tag="VW325_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9734105.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            complement(25674..25970)
                     /locus_tag="VW325_000666"
     CDS             complement(25674..25970)
                     /locus_tag="VW325_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9734106.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            complement(25992..27056)
                     /gene="pepA"
                     /locus_tag="VW325_000667"
     CDS             complement(25992..27056)
                     /gene="pepA"
                     /locus_tag="VW325_000667"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9734107.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            27457..28158
                     /gene="galE"
                     /locus_tag="VW325_000668"
     CDS             27457..28158
                     /gene="galE"
                     /locus_tag="VW325_000668"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9734108.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            28581..29540
                     /locus_tag="VW325_000669"
     CDS             28581..29540
                     /locus_tag="VW325_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734109.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTNDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            29530..30486
                     /locus_tag="VW325_000670"
     CDS             29530..30486
                     /locus_tag="VW325_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9734110.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATSLVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            30483..31235
                     /locus_tag="VW325_000671"
     CDS             30483..31235
                     /locus_tag="VW325_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9734111.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINIASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            31331..32296
                     /locus_tag="VW325_000672"
     CDS             31331..32296
                     /locus_tag="VW325_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9734112.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLAEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(32521..32763)
                     /gene="yidD"
                     /locus_tag="VW325_000673"
     CDS             complement(32521..32763)
                     /gene="yidD"
                     /locus_tag="VW325_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9734113.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(32763..33485)
                     /locus_tag="VW325_000674"
     CDS             complement(32763..33485)
                     /locus_tag="VW325_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9734114.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(33472..34041)
                     /locus_tag="VW325_000675"
     CDS             complement(33472..34041)
                     /locus_tag="VW325_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9734115.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(34060..34788)
                     /locus_tag="VW325_000676"
     CDS             complement(34060..34788)
                     /locus_tag="VW325_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9734116.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(34788..35528)
                     /gene="xerD"
                     /locus_tag="VW325_000677"
     CDS             complement(34788..35528)
                     /gene="xerD"
                     /locus_tag="VW325_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9734117.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(35519..35980)
                     /locus_tag="VW325_000678"
     CDS             complement(35519..35980)
                     /locus_tag="VW325_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9734118.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(35977..36498)
                     /locus_tag="VW325_000679"
     CDS             complement(35977..36498)
                     /locus_tag="VW325_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9734119.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(36474..37445)
                     /locus_tag="VW325_000680"
     CDS             complement(36474..37445)
                     /locus_tag="VW325_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9734120.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(37442..38236)
                     /gene="racE"
                     /locus_tag="VW325_000681"
     CDS             complement(37442..38236)
                     /gene="racE"
                     /locus_tag="VW325_000681"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9734121.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(38443..38691)
                     /locus_tag="VW325_000682"
     CDS             complement(38443..38691)
                     /locus_tag="VW325_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9734122.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(38947..40572)
                     /gene="treC"
                     /locus_tag="VW325_000683"
     CDS             complement(38947..40572)
                     /gene="treC"
                     /locus_tag="VW325_000683"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9734123.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(40750..42717)
                     /gene="treP"
                     /locus_tag="VW325_000684"
     CDS             complement(40750..42717)
                     /gene="treP"
                     /locus_tag="VW325_000684"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9734124.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKGLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            42902..>43061
                     /locus_tag="VW325_000685"
     CDS             42902..>43061
                     /locus_tag="VW325_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9734125.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEE"
BASE COUNT        12521 a         9991 c         8261 g        12288 t
ORIGIN      
        1 aaaatcgtca acgctcttca attaccttat tccaacgcaa aattggaagc gaccaataat
       61 ctcatcaaac ttatcaaacg aaacgccttt ggatttcgga actttgaaaa cttcaaaaaa
      121 aggattttta tcgctctgaa catcaaaaaa gaaaggacga attttgtcct ttctcgagct
      181 tagctgactt caacccacta cagttgacaa agagcccaat attctgaaaa caaaaaaaga
      241 cgcgagtttc cacaatctca ctcatggaaa ctcacgtctt tcaaaaaata gggtgaattg
      301 aaaactagaa caagacacta gattttctaa atgatgtcta gtaaggattc taaatgctac
      361 accaaattgc tcacatctta tttcaaacca cgggactccc acaagaaagg aaaatcctat
      421 aaaaattctc tctctgagga gaaaggagcc tcaactacag gtgtaaacta ttatcaattg
      481 aaaacaacag tttttacact caattagatt ttttaaggac aggatcactt tcgcttgact
      541 gtagtttgtc atttttttga taaagttagc actttttcag aaactgtttt gaaagtctca
      601 atgatatagt ctacttcttc atcgcttaat ttagtatgaa gagggagggt aatttcattc
      661 tcaaagaagg cataggcctt aggatagttc gtcatatcaa atccaagatt cttataggct
      721 gtcaagagag gaagcggttt gtagtgaaca ttacttgcaa ttcctgcttt agccaattct
      781 tggatgatga ggttgcgttc ttctaggctt gctccttcta cacgggtgat gtagaggtgg
      841 cgtgaagatt cgacagtttc agtcttgtgt gccaaaggat ggatgcgaga acctgcaaaa
      901 ccactatcat agcggtccac aatgtcctta cggcgttgca acaaacttgg atagcggtcc
      961 aattgtacca aaccaagtga agccatgata tcggtcatgt tgcacttata ggctggtgta
     1021 acgatatcgt attcccatga ccccagttgc atcttggcaa gagcatcctt agtttgcccg
     1081 tgaagggaaa ggatttggaa ttccttgtac atctcttcgt catcaatcac tggattggct
     1141 ttccaagtcg cacttccacc ttctgccgtt gtaaagttct taacggcatg gaatgagaag
     1201 gaagtaaagt cagcgataga accagaaggt tgtcctttat aagtagatcc caaagcgtgg
     1261 gcactatcag agacaatgac aatacggtta aaggcctttt gccacttgct tgaagcggta
     1321 aagaagtcac gttttttctc cacgacttgg aacaaacggt cataatcgca aacaatccct
     1381 gcgagctcta ctgggataat caccttagtt ttctcagtga tagcttgctc aagcaggtca
     1441 tagtccatct caaacgtatc tgcttggata tccaccatga caggggttgc tcccacgtgc
     1501 gtaatgacac tacatgaagc cgtataggtc atggctggaa cgatgacttc atcaccaggt
     1561 cccacttcca aaacgcgtaa aatcaactcc agagcggctg tcgcagagtt gagacaaaca
     1621 gtcttaggtg tctgtgtgta aagagacaag cggcgctcca gttcttttgt tttaggacct
     1681 gttgtgatcc aaccagaacg cagggtatcc gctacttcag caatttctgc ttctgtgata
     1741 tcaggcggtg aaaatggaat attgtaattt ggcattgatt tgctcctttt atctgtactc
     1801 attttcttat gactacttta ttttagtacc tcaaacacgg tttgaaacat gattttgatg
     1861 tccccaaaga aactaaactc tcggagatag gcgaggttat agcgcatctt ttcaggaaga
     1921 acatgctcca cataggcctg atcaactgac agacctttct ccgtcatttg actgatgatg
     1981 gtgtcctcat ccttgtagtt gatgctggct ggagaggtaa ttcctgcttg caagagcaag
     2041 gttgccatca tttcagggct atactgctct gtataacgtg gcacttcagg tcgtgtaccg
     2101 acaaaggaca tctcaccttt aaggacattg accaactgag gcagttcgtc caaacggaca
     2161 cgtcggatga aatttccaac cttggtaata cggctatcgt tagcagaagt caccagactt
     2221 ccttttttat ccgcatccgt caccatggta cggaacttcc aaatcttgaa ccgacggttg
     2281 tactgggtca cacgctcttg cttgtaaatc actggcccct tgctatccaa cttgatccaa
     2341 atgctcaaga tgagaaagat gggagaggtt agaaccagta agaccaaggc caaaacccag
     2401 tccaagcaac gcttgaaaat cagcgaaccc ttccttttag agacaagctg gtagtaagac
     2461 tcaacctcgc ttgatttcat ttccacaggc aagtcttccc atttcagcat gctgttctcc
     2521 tttgttttta ttatactatt tgtcaacatt tttcattata ccacaaaatg gaaaaggcgg
     2581 tgaaagaaat ctgtaatttg aaggatttcc ttctagtact caatgaaaat caaagtacag
     2641 ctttgagatt gcagataaag ctgacatggt ttaaagagat tttcaaagag tatcggccat
     2701 ggcccctgcc tgcaaactat acatcttgtg ataagttcct cccagagcca agagttcctc
     2761 atgggttcca ctctcgataa tgcgtccctt atccaagaca tagatgcagt tggcgtcttg
     2821 aatagtagaa aggcggtgag cgatagcaat agttgttcgg ccctgtctca tcttcgccag
     2881 agaagcttga accaaacttt ctgtttcaga gtcaatattg gctgtcgctt catccaaaat
     2941 caggatttta ggctgactgg ctactgttct agcaaaggcc agaagctggc gctgcccagt
     3001 agagaaactc gacccacgct cagaaacagg ggaatcataa ccctgaggaa gatcctgaat
     3061 aaaggaagct gcatcgacaa aagtagctgc gtcctttacc tgctcatcac tgatctcttg
     3121 gtacatggag atattggact tgattgttcc atgatagagg aaaggatcct gcaagaccag
     3181 accgatgttt tttctcaact cttcctgact gtagttcctg atatccacat catccaagag
     3241 aactaggcct gactgaaatt cataaaaacg catgaggaca ttgataatag acgatttccc
     3301 agaacctgta tgacccacaa aggcaatggt ttcacccttc ttaacagaaa aagaaatatc
     3361 atccagaatc gggtgcttgc catcatatga gaaacataca tgttcaaacc gaatattgcc
     3421 ttcttggatt ttggcttgcc catcattttg aagcggctca taggttctct cgtcaatcaa
     3481 ggcaaatact cggcctgcag aaaccataga cgtttgcagg gttgaaaagt tttgcgtcac
     3541 cttaatcaag gggtcaaaga gacggttgat gtactgaata aaggcataca tggttccagc
     3601 tgttattccc agataaaggc cacgatagcc aaagtaggcc atcaagaccg cataacctaa
     3661 gagtttcagc aaactcatgg cagggcgcaa aaagagggca tccaaggcta cagaacggtt
     3721 ggcataggcc aagtgttctt ggttaatctc atcaaattcc gtctgcaggc gtttctcttg
     3781 attaaaggcc tggataattc tgattccctc gatgttctct gccagcttac tattgatatc
     3841 tgacaagaga cttctggttt tctcgataat cttgactgac tttttccggt agatattgac
     3901 caaaaggaaa atcaagggaa gaaagagcag gactaaagct gtcaaacgaa aatccaacac
     3961 caacatggta taaagggttg tcagaaagat aaaaactgct gagataaagc tggataaaat
     4021 cccagaaaac atatcactaa tcgtctcggt atcgttggtc aaacgagaaa cgatagaacc
     4081 tgctggcgtc ttgtcaaaat aagacatgcc cagcttttcc atattggcaa aggcatcccg
     4141 acgaatatct ctaacgatgc tgtaggatac ccgcgcaaag agaagattgc cgacatactg
     4201 aactacagtt tgtaggatat aaaggccata gtaggccagc aaaacggtaa tggccagttg
     4261 gttaagatta ctcagatact ggtcgataaa gtgggaagcc acaagaggaa tgacactttt
     4321 aatgaccgtc gtcgctagta gaaaactgag tgccaaaaag gtcaggagtc cataaggctt
     4381 gagataggac atcaggcgct tcaatacatc ccattgttct ttcttattcc gcatcttctt
     4441 ctcctttcat ttccaactgc tgagactggt aggtttgggc ataccagcca tccagggcta
     4501 gcaagtcttc gtgcctgcct cgttcgatga tttgaccatt ttgcaggact aaaatcaaat
     4561 ctgcatgaac aactgcactc aggcgatggg ctgtgatgat ggttgtcttg tcctttcgca
     4621 tctccttaag gttgtcgata atcgcatact ctgtcttggc atccacggct gataaggaat
     4681 catccaaaat caagatatca gggtctaaaa tcatagcccg actcatggcc agacgctgct
     4741 tttgacctcc agaaagactg actccctttt caccaatcag cgtatcaaat ccctgaggca
     4801 tggctacaat gtcttggtaa acttgggcta gcttggtcgc ttcctcgacc gctgaaagag
     4861 gcagcttaga attgccaaag cgaatattgt ctaaaattga agtcgcaaag aggaactggt
     4921 cctgaggaac atagcccatg agactgcgaa gatctgtcag acgatagtcc cgaatatcgt
     4981 gaccgtttag gtaaatggct cccctatcca catcgtattc acgcaagagg agtttgatca
     5041 aggacgtttt cccagagcct gtctgtccaa ccaaacccag agtttgccct ttttccaaac
     5101 taaagtgaac atttgtcagt gtttcctcat tttcaaaggc aaagctatca atggcatact
     5161 gcaagcgccc attttcaata ccatctaaag gaaactccgg gtcttgtaca ggtgattcct
     5221 gagacaaaag ttcctcaatc cgctggtaag aaaccttgcc tcgctgagta atattaaaga
     5281 ggaaaccgat ggccataaga ggccaaacca gcatatccaa gtagctgata aaggtaacta
     5341 gattcccaac cgtgatttgc ccttcctgaa ccatcaagga gccgaccaag agcgttaaaa
     5401 cataggagga accaacaaat aagagaacca tggggtcaaa gagactatcg tatttcatgg
     5461 tttggaggtt cttttggaag gtcaattcat taactgcctg gaaagacttc aactcgtccg
     5521 cctgataccc gaaagacttg gtcactttaa tacctgatac agactcctgc accttgttat
     5581 tgagttcaga aaaggcagcc tgtgattcac caaaagcctt gtgggtcttt ctccctagac
     5641 gactggtcgc ataagccatg aaaggcaggg gaagaatggc aaccaaggtc atctgccatg
     5701 aaatgctaaa gagcatggtc agcaaggtca ctaaagccgt gattgaggca tccactgctg
     5761 acatgactcc gccacctgcc agacgcgtta gggcattgat atcgttggtg gcatgtgcca
     5821 tcaggtcccc cgtccgatag gtttgataaa aagctggaga catttttgtg aaatgctcaa
     5881 ataagcgaga gcgcataatc tgtcccaaac ggtaggaagt cccaaggata tacatacgcc
     5941 aaacatagcg cagatagtac atcccaaagg ctgcaagaag caagtaaaat agggcaagaa
     6001 ggaggacctg ctgggttaat tgccccgatg tgatggcatc aatgacccgc cccatgacca
     6061 tagggggaat gagattgagt acggaaacca agaccaaagc cacaatcccc actaaataac
     6121 ggcgtttttc taacttgaaa aaccaccaaa gtttttgaat aatggacata aaatcccttt
     6181 ctgattgcaa atggaaacct gaggccaata ctcaatgaaa atcaaagagc aaactaggaa
     6241 actagcctca ggctgctcaa agcactgctt tgaggttgta gatagaactg acgaagtcag
     6301 ctcaaaacac tgctttgagg ttgcagatag aactgacgaa gtcagctcaa aacactgctt
     6361 tgaggttgca gatagaactg acgaagtcag taacatatac atacggcaag gcgacgttga
     6421 cgcagtttga agagattttt gaagagtaca agaccccagg tttttcttat tcataagtta
     6481 cgactgtaac agcacccttg tcatactcag ctataaagat attggcaaca ttttcatgtc
     6541 cttgtttgct gaggttatca agcagccact cttcgctacg accaatcgat tccaaaacat
     6601 caacttggat cacaccgtca gtcacaactg gatacttagg attttcatct cccatttgga
     6661 ccacgatgag ttggccattt tgctcttgca cagctcgttt aacttgcttc atctggaaaa
     6721 tcccttggct acgaagtttg agggctacat ccgctgcaga caagccaact gaacgacagg
     6781 cttctgggtc gatctgccca tttttgatga ggagagttgg tttcccatca atcaagcgtt
     6841 tgacaaaacg aacattattg ttgagccatt tgagggtcaa gaccaaaatc gtccacatca
     6901 tcaaaatcac tgcgtattgc agaatactaa ttgaactatt gtaaatcacc ccaccgatga
     6961 taccaccaag aacatagttc tgaatttggt ctgtcgcaga gttaggcgct aggtttcctt
     7021 ttcctgtcac attgataaca aaaacaagag agaagagccc caaggccagt ttgattaaaa
     7081 tttccatata attgagtgtc atttgtttac ctccaccaat tcaatagcgt ctgtttgatg
     7141 caattctatt ttctctaaaa gatacttgtc tggttggctc ccgttcatag cacgatagac
     7201 atttgaacct accttgacaa gcgctccatc agtggcagct gaagtattga cgtaaacttc
     7261 tgatttatcc actcccaagt ctttggaaac aacctctatg aaatgaaggg aggtttgaaa
     7321 ttgattgtta gaggcttgat tggtctggta ttttgaaatt gtcaccaaaa gcatggccac
     7381 taaagtcagg gccaaaatca tgaccaattc ccgaaactta gtcccctttt tatcacgata
     7441 ggccttaaaa gcaaaaaatc ctgtgatagc taggagaaca atcccaaagc caatcataat
     7501 cccattctgc tgactgattt ggctaagcac atagtcatat gagtaaaatt tcatttattt
     7561 tcactccttt tcataaaatc attcttaatt ataaacgaaa gagggtgatt tttcaaacca
     7621 tacattgggg aaatagacct ttttttggta taataaaatc tataatctga atgaaaaagg
     7681 taactttatg aaacttatct catggaatat tgattctctc aatgctgccc taactagtga
     7741 ctcagctcgc gcgaaattgt cccaagaagt cctacaaacc ttggtcgctg aaaatgctga
     7801 tattatcgct atccaagaaa ccaagctttc tgctaagggt cctacaaaga aacacttgga
     7861 aattttagaa gaactcttcc caggctacga aaatacgtgg cgctcttccc aagaacctgc
     7921 ccgtaaaggc tatgctggaa ccatgttcct ttataagaaa gaactcacac ccactgtcac
     7981 tttcccagaa atcggtgccc cttctaccat ggacttggaa ggtcgtatca tcactctaga
     8041 atttgataaa tttttcgtga cccaagttta cactccaaac gctggcgatg gtctcaaacg
     8101 cttggaagaa cgtcaagtct gggatgccaa atatgctgcg tacttggctg agctagacaa
     8161 agaaaaacca gtccttgcga ccggtgacta caatgtagcc cacaatgaaa tcgaccttgc
     8221 aaatcctgcc agcaaccgtc gttcaccagg atttaccgac gaggaacgtg ctggatttac
     8281 caatctcttg gcaactggat ttacggatac cttccgccat gttcacggcg atgtcccaga
     8341 acgctacact tggtgggcac aacgcagcaa gacttctaaa atcaacaata caggctggag
     8401 aatcgactac tggctcacaa gtaaccgtct cgcagacaag gtcactaaat ctgatatgat
     8461 tgactcaggt gctcgccaag accatacacc gattgtcttg gagattgaac tctaaggaga
     8521 aaacgaatgg actatcaagc tgtcattcct gaatttgtag tatctgacat cgaaaaatca
     8581 cgccacttct actgcgattt gctgggtttt accatcgaat acgagcgacc agaagaaaac
     8641 ttcctcttct tatcgcttga agattgtcag ctaatgttag aggagggaac aaaggatgaa
     8701 ttagttgatc tgacctatcc tttcggacgc ggtgtcaata tttcctttgg cattgtagat
     8761 gttcctcagc tccaccaaaa actgctggaa gctgactatc ctatccatcg tcccctgaca
     8821 aaaagagaat ttcgagtagg agatagcttt atatatcccc atgaatttgc agtcttggat
     8881 ccagatggct attttttaag atttagcgag tagagaatag aaaaaatcct tcaaatcaga
     8941 aaaaggcata ttgtcaggca tttaaaaacc taataatatg tccttttatg atgaaaaaac
     9001 cttctaaatg ttagctaata agttaacttt tgcccatttt tattatattc gttcgctttt
     9061 taaaccaaaa cactaacaaa acatttgatt tccaaagtta tttagtgtaa aataaaaaca
     9121 ttggctcacg cctatttgaa attgaatatc gttttacttg tttatgtcgt gaattggcac
     9181 gacgtttcta caaggtgccg gaacacctaa caataagtaa gtcagaagtg aggtatggtc
     9241 gtttttgcca tgcctatttt ggggacttac ttagagatta agtaggtcct ttttctatcc
     9301 gttttactgg gtagaaatct gaaacaagca aatggattgg cttttagact ttaggaggtc
     9361 ttatgaaatt attagaagag cgcatcctca aggatgggca tatcttgggt gataacatcc
     9421 tcaaggtgga ttccttttta acccaccaag ttgactttag cttgatgcga gagattggta
     9481 aggtttttgc ggaaaaattt gcttctgctg gcattaccaa agtcgtgacc attgaagcgt
     9541 caggtattgc cccagccatt tttacagctg aagccttaaa cgttcccatg attttcgcta
     9601 aaaaagctaa aaacatcact atgaacgaag gaatcttaac tgccgaagtt tactccttta
     9661 ccaagcaagt gaccagtacc gtttctatcg ctagaaaatt cctctcacca gaggacaagg
     9721 tcttgattat cgacgatttc cttgctaatg gccaagctgc caaaggcttg attcaaatca
     9781 tcgaacaagc tggtgccaca gtcgaagcta tcggtatcgt gattgaaaaa tctttccaag
     9841 aaggccgtga tttgcttgaa aaagcaggct accctgttct atcactcgct cgtttggatc
     9901 gttttgaaaa tggtcaagtc gtatttaagg aggcagatct ctaatgcaaa ctcaagaaaa
     9961 acattcgcaa gcagccgttc ttggcttgca gcacttacta gccatgtact caggatccat
    10021 cctggttcct atcatgattg cgacagccct tggctattca actgagcagt tgacctacct
    10081 gatttccaca gatatcttca tgtgtggggt ggcaaccttc ctccaactcc aactcaacaa
    10141 atactttggg attggactcc cagtcgttct tggagttgcc ttccaatccg tcgctccctt
    10201 gattatgatt gggcaaagcc atggtagtgg tgctatgttt ggtgccctta tcgcatcagg
    10261 tatttacgtg gttcttgttt caggcatctt ctcaaaagtg gccaatctct tcccatctat
    10321 cgtaacagga tctgtcatta ccacgattgg tttaaccttg attcctgtcg ctattggaaa
    10381 tatgggaaat aacgttccag agccaactgg tcaaagtctt ctacttgcag ctattaccgt
    10441 tgtgattatc ctcttaatta atatctttac caaaggattt atcaagtcta tctctatttt
    10501 gattggtctg gttgttggaa ctgccattgc tgctagcatg ggcttggttg acttctctcc
    10561 tgttgcagca gcacctcttg tccatgtccc aactcccctc tactttggga tgccaacctt
    10621 tgaaatctca tctattgtca tgatgtgtat catcgcaacg gtgtctatgg ttgagtctac
    10681 tggtgtttat ctagccttgt ctgatatcac gaaagaccca atcgatagca cacgccttcg
    10741 caacggttac cgcgcagaag gtttggccgt acttctcgga ggaatcttta acaccttccc
    10801 ttacacagga ttttcacaaa acgttggttt ggttaaattg tcaggtatca agactcgcct
    10861 gccaatctac tacgcagctg gtttcctggt tctccttgga ctccttccta agtttggcgc
    10921 ccttgctcaa atcattccaa gccctgtcct tggtggtgcc atgctggtga tgtttggttt
    10981 tgtgtctatt caagggatgc aaatcctcgc ccgtgttgac tttgctaaca atgagcacaa
    11041 cttccttatc gcagctgtat caattgctgc aggtgtcgga ctcaacaaca gtaatctctt
    11101 tgtcagcatg ccgacagcct tccaaatgtt cttctcaaac ggaatcgtcg tagccagcct
    11161 acttgccatt gtcctcaatg ccgtattaaa tcataaaaag aaataagaaa aagaggtgtg
    11221 agcctctttt ttgtgtgaca aaataaaaac atctactcat atatactagt ctcatcgtcc
    11281 tgaaaatcat ctgcatcaag aacaatttca caactcttat acttttctct tacaagtcgt
    11341 tcggcttcat ctggattttc agcctctatg cttactaaac gggttagagt ttctgtaatt
    11401 tctactgtat actttttcat cttaagtttc tctccaataa ctcacttatt agcaataatc
    11461 tatgttttgg gctttccaac cactctggta attcatctcc atattcccag ctatattttt
    11521 tattcaataa agaaagttgt ttctcatcac caaactccat attttcagat aaccacctca
    11581 cacaatcagg gtctgataaa gtataaacca ttacacataa gttttcataa ttactctctg
    11641 tttcataatc aggccagatg acttttaaga aatcatacac ctcttctgat gcaacaagtt
    11701 gtttcgtttt cataatttcc gatactttcc tctaccttta aattctatta tttctttgtc
    11761 tcttaatatt tgaagctgtt gcctaatctt ttctttgata tgattgttct taacaaagat
    11821 attttttagg tcactttcaa aacgatacat atcttcaagg gtaaattctg aaccctctat
    11881 cttatctata caatttagaa tttctattgt ccaacctctt actgaaagag agttcttcct
    11941 taaaaataaa ctttgcttaa attcttttgt aactttttct ggatctctaa cttgtccatt
    12001 tttcacaaga aatattcttc ctttagaagg tacttgtgat aaatcaatgt tacaaccaat
    12061 ccaacctgct cgtctagcag ttggcgcaag tggcttcctt tgaataattg atttcggtgt
    12121 aacaaattgc tttggaagga ccagaaaatt atttacctca aaattttttg tataagttaa
    12181 aaaaaagaaa ttaggattat tatctgcctg cacacgcttc atcatcgttg cataagcacc
    12241 atcattgatt gttgatgaaa aatttccttt tttgctcttt agttcaaact cctcactaca
    12301 atgattacag taaaaatctg ctacaggccg attattttca aaatgattta agggattgtt
    12361 cccataatta gggcaataag actgacgata aacccaatcc tccgttaaga tacgtgcctt
    12421 ttggctatta cttttatagg tttctactaa ttctaaatta aagtgtaatt ccatttaaaa
    12481 attcctcact aatatgagaa ctcactcgat ataagcaatt aaaatctact ataactagta
    12541 taccacatat atgtacctat tacatttgta tttcaccact ctcttttata tttccttcta
    12601 acatatctca taaattaaaa atgagtgaga taaatttatc ccactcacct ttctttatat
    12661 ccttacccgt ctttcctcag acctttggcc tgtgacaaac atggtaaagg ttgctttaca
    12721 gacatttctg ccctcttgat tggtgatatc cacatccatc acacaggttg tacgaccttg
    12781 atggacacat tctcctttaa tggtcaatac atcgtcgagt tttcctgcct taaggtagtt
    12841 gatagaggat tggagtgtca ccccatcaag tcccagcgag ataaccacca aaccactgat
    12901 ttggtcacat agcgtgaata gatagccacc atgggcattg ccatagtagt tgagcgacga
    12961 gttcactact ttggtcgtca ccacaacgtg accatctctc attttttcaa tttcgtaatt
    13021 ttcaaaggca gatatagcgt caaaatgaaa gtctttcatc gtttcctcct gatatttcaa
    13081 acgacactat gatactactt tttataggac tgtgcaagga gaaagctcct agtctagtga
    13141 aattccgacc tgttccacaa agcgcataaa ggctgtctgc ccttgttctg tctgcttgta
    13201 gactcctgca tcctcaagta cacgcacaaa gatagcaccc acagagtcct tgacgattgc
    13261 aagggctttt tctttatccg ttaggtctgg atgttgggat ttgagttggt ctgcccactc
    13321 ctgatgataa tcggcaactg taacagcttc tcctacaaga tagctagcga cttgctccac
    13381 ttcttctttc agacgtggtg gcaagattgc caagcccatg acctcaatca agccgatatt
    13441 ttccttcttg atatgttgga catccttgtg gggatgatag atgccatcag gatgctctgc
    13501 tgaagtctga ttgtctcgca agaccaagtc caactcaaac tgtccatcac gcttacgcgc
    13561 tattggtgtg atggtgtgat gcggtgtccc gtctgtctct gctaaaatct gcacactaga
    13621 atctgaatac tgacgccatt cctggaaaat cttatcagct aagttaatca aatcctcttt
    13681 ggaatccgaa gtcaagcgta ggacagacat tggccacttg ataattccag ccttgacctg
    13741 ctcaaaacca gcaaatcgga aggtcttttg caagggagcc aattccatag gaaatacgtg
    13801 acggcctccc tgataatgat cgtgagttag aatagagccc cccacaatcg gcaggtcggc
    13861 attagatcca gcaaaatatc ctggaaactg ctctacgata gccaacagac gttcaaaact
    13921 ctgacgacta atggccatgg gacgatgctg gccatctaaa aagatacaat gctcattaaa
    13981 gtaagcatag ggcgaatact ggaaccccca ttcctgacca accatttcaa aacggataat
    14041 ccggtggttg ctgcgagctg ggtggttgac ccgaccatgg taaccctcat tctctaaaca
    14101 aagttgacac tgaggataat tactagcttg caccaacttg gctaccgcaa tctctttcgg
    14161 atccttttca ggcttagaga gattgatggt aatttcaagt tctccataat cagatggaac
    14221 acgataagcg atatttttag caatggcctt gagtttgatg tagtcatttt tctgactgag
    14281 ttgataaaaa tcctctatcg cttgctcagg agagtaggca taggcttccc agaagtcacg
    14341 attgacctga ctcggacaag gagtcaccaa gtccatcagt tcagtaccaa ggatttcacg
    14401 cgcagtctga ctatcctcaa tcgtctctaa tcgaacggct tcctcaacta gctggtcctt
    14461 gaggtcaatc aatttatcca gattggtctc aacttccaaa acaccttctc ccactcgtgc
    14521 caaaacacga ttggtcaggt agattcgatc catttcctca aatgaacttt cagaaatgac
    14581 atgtgttaca aatttatcta ctaaggtcac tatagagcct ccttttgact agtcaaggac
    14641 gcgagtgcca cctgcaactt cagcgatata gaagcttgga gcgtatccaa ctacttcctc
    14701 gtagtgtttg cctacagctt ccttaaaggc ctcaacagta tctttttgaa ccaaggcaat
    14761 ggcacagcca ccaaaaccag cccctgtcat acgagcaccg agaactcctt cttgtgccca
    14821 agctgtgtga acaagggtat ctaattccaa accagttact tcataatcat gctccagaga
    14881 aacgtgtgac gcattcatca aacgaccaaa tgtttccaaa tcacctgctt gaagggctgc
    14941 ttgagcttta agggtacgtt ggttttcaag cacagcatgg cgagcacgtt tcaaacgatt
    15001 ttcatcttta attagatagc tatattggtc aacggcccac tcgtccaatt cacccagggt
    15061 ctgaatatcc aaggcaactt gcaattcttc cactgctttt tcacactcag cacgacgttc
    15121 attgtattta gagtccgcca attcacggcg tttgttggtg ttcatgataa caacgacatt
    15181 gtccttcaaa tcaagtggca ccaaatcata ctctaaggtg ttggtatcaa ggtaaatagc
    15241 acgttgatca gcccccatac caatagcaaa ctggtccatg ataccagagt tgactccgat
    15301 aaagttgttt tctgtttgtt tgccgatttt aaccaaatcg agacgctcta attttaaatc
    15361 aaagagatgc tctgccacga ctcctgtcaa gagctccaag gatgctgaag aagacaagcc
    15421 agcaccattt gggatattcc cataaacata aaaatcaaaa cctttgtcaa tcacgtgccc
    15481 agcttcttgc aagaaatgaa gaaccccttt tggataattg gtccagttgt gctctttttc
    15541 aaacttgagg tcagcgagag gcacttcgat aatgcccttg tcctcaaagt tagctgagta
    15601 gaaacgcaag acttggtcgt cacgcttacg agctgcaccg taagttccca aggaaatagc
    15661 agcaggaaaa acgtgcccac cgttgtagtc tgtgtgttca ccaatcaaat taatacgacc
    15721 tggtgaaaag aaggtttggt ctgcttcttg accaaaaaca gcaagaaagt ctttgcgaag
    15781 gacttcagta gtaagatgtt gtgccatatg aattctcctt tgactgtccg ttaagccatc
    15841 ttaacgtgta atcgttttct tctattgtat ccaaggaatt tcccaaggtc aatccttttt
    15901 actaaaattt tagtaaacta tcagtaaaaa gaagaaaaat atgatatact aacctaatca
    15961 aggaggattt atggctacct taaaagacat tgcacagcta gcctctgtct ctatcgcgac
    16021 cgtatcccgt gtcctcaacc gcgaccagag cctatctgtt acagaagaaa ccagacaccg
    16081 tattttaacc gttgctgaag agctgggcta caccaagcac ctcaagacag gcgagtccca
    16141 taaacccaag caaaagattg ccattattca atgggtcagc gaacaagggg agctagacga
    16201 cctctactac taccagattc gcctaggaat agaaaaaaga gcccaagagt tggactacga
    16261 tatcttgcgc tattttaatg accatccttt taccctaagc gaggaagtga ttgggattct
    16321 ctgcatcgga aaatttagcc gagctcagat ttctgccttt gaagaatacc aaaagcctct
    16381 tgtatttcta gacagcgata cactttccct gggacatacc tgtattatca cggattttta
    16441 cactgctatg aaacaggttg tcgattattt cctcagccaa ggaatggacc gtatcgggat
    16501 tctaacaggc cttgaagaaa caactgacca agaagaaatc attgaggata agcggctgga
    16561 aaatttcaga aactacagtc aagcaaaagg aatctatcat gatgaactga tctttcaagg
    16621 aaactttact gcccagtctg gctatgactt gatgaaggaa gccattcaga acttgggaga
    16681 ccaactccca ccagcatttt tcgcagccag cgatagttta gccatcggtg cccttcgtgc
    16741 gctccaagaa gctggaatca gcctaccaga ccgtgtcagc ctcatttcct ttaacgacac
    16801 cagcctgacc aagcaggttt atcctcccct ctccagcatc acagtttata ctgaagaaat
    16861 ggggcgagca ggtatggata ttcttaacaa ggaagtcctc cacggtcgga aaatccctag
    16921 cctgaccatg ctgggaacca gactgacatt aagagaaagt accctaaatc aagaatagga
    16981 taacataaaa aacgaataga gttctaaaac tcctattcgt tttttattcg attacaatca
    17041 tagacttaat tgtcttacgt tcatccatat ctttataggc ttggtcgata tcttccagtt
    17101 tataacttga agtaaagacg cgacctggat tgatatcacc atcaaggacg gcttttagta
    17161 aaaattgctt atcgtatgtt gtagcagaag ctgccccacc tgctacagag atattttgca
    17221 taaatgtcga accaagagca cgattattat agtgtgggac tcctacaaag cccatacgcc
    17281 ctccattatg aagaacacct agcgcctgtt ctatagcagc ctccgtacca acacattcaa
    17341 gtgctgcatc tgctcctcca ccgaggattt cacgcacctt ggtaattcct tcttgaccac
    17401 gttctgcaac aacagctgtc gcacctgact ccatagccat cttttgacgg tcttcatgac
    17461 ggctcataag gataatttgt gatgctccac gcatcttagc cgcgatgaca gcacattgac
    17521 caacagcccc atcaccgata acaacaacct tgtccccttt ttgaacattt gcaacacgcg
    17581 ccgcatgata gcctgtcggc atgacatctg caagagtcaa aagggacttg agcatccctt
    17641 ctgtatagtc agaaggttga ccagggattt taaccagcgc ccagtttgca tagtggaagc
    17701 gaatatattc tgcctgaaaa tcacccccca aattattgcc aatatgattg tcgcaagaac
    17761 cgtcaaatcc agcaagacag gcatcacact caccacatcc atgtgtaaaa gggacaatca
    17821 caaaatcacc tggtttcacc gtcgtaatgg cttccccagc ttcttcaaca atcccaatcg
    17881 cttcgtgtcc actatttttg tgtccagctt tcgtttctgg attacggtac ctccataaat
    17941 ctgaaccaca aacgcacgca cgaaccacac gaataatcac atcatccgct tctattattt
    18001 gcggacgcaa gtccaacctg acctgccttt gtatatactg ctgatttcat ttaaaatttt
    18061 ccttccttat aaagtttaat tttgagattt aaacgattta aagctgtctg tagctgagac
    18121 aagcgctcct ctaatttttg cttttcctct tctaaaatac caagcctctc ctctctcgtt
    18181 tcatctccct tttggtagag cgacatatag tcaactaaac tatctacaga gacacccgcc
    18241 gaacgaaaac acttaataaa ttccagcgct tcgatatcct gatcttgaaa atcacgaatc
    18301 ccagtagcag tacgagtaat cggtggcaca agaccaaccc gttcataata ccgaatcgta
    18361 tccgctgaaa ttcccaacaa atcactggca gatttaatat tcacacgcta acctcctctc
    18421 atttcattca ctgaattcac gctattctaa ctcacttatc ttattatata actttgagtt
    18481 gactccaagt caagcatttt gtatctaatc gaaaaatttt tatcattaac aatcatatat
    18541 ctggagttta tacaatgctc ttctcttacc aatttttgaa atatttaaat aataaagaaa
    18601 ttactctatt ccaaataaga tagtacccaa gtatttttca cttttttcta atgttgatgc
    18661 tcataactcc gttccaagtc acacaccttt cgcttatggg tttggtgagt ttctaggtca
    18721 gcatcaattt caatggtaat attttgaaaa ccacaatctt ttaggaaaat tcgaatagac
    18781 tctttacaag tttccatatg ttccatttct tttagacaaa catggacaat ggcatttttt
    18841 tccaaagcat ccatagtcca gagattaagc tgattaaggc tggccacatt gtccaatcgc
    18901 tccaggtcac tctttacttg cttgatatca agaccttctg gcacagcatc caagaaaatc
    18961 ttgagtgtag accaaaaacg tggaagggct tttgaaagaa taaagaaaga aatgataagg
    19021 gacaaaagag gatctaggat ataccagtcc gtaaatcgaa gaacaatcgc catcaggata
    19081 acagctaccc accctagcgt atcttccaga aaatgcagac tcagaataga ctcattcttt
    19141 gtctttccct taccaaccac cagactcgct aacagattga tagtaatcgc aataattcct
    19201 aaccagagaa tcccctcatc attgactggt tgcggatgca aaatcttcgt gacattttcc
    19261 aaaatgacta gaacagagcc tgttacgaga atcacagctg ttaccacggc tcctagcagg
    19321 ctaaaccgct tatagcccaa ggtgtactga ttgtcttctt cacgattgga gattgtttct
    19381 agaaaagctg atattccaat tgcaatcaca tctcccaagt catgcacaga gtcagcaaga
    19441 acagcgctag aaccaaatac tccacctgca ataaactcaa caatggcata agtcaaattt
    19501 aagaaaaaag ccacccaaac agcatatttt gccttcatat ttctcattcc tttgttataa
    19561 tagatttatg aacaccttgt tcattatcat tatccactaa aactcaaaga aaggatagaa
    19621 gcaaaacgtc agctttattc agttctgaac aatttgcctc aagtgtccat atgactaaca
    19681 ttgaccgccg tatcagcaaa accaaaaaag ccatctatca agcttttata caacttttga
    19741 atgctaagga ctacgagacc actactgttc aggatatcat tgatctcgca gatgtgggac
    19801 gatccacctt ttactgtcac tatgagagta aagagcttct tctggatcag ctctgccgct
    19861 acctcttcca tcatcttttt gaaagagagc aagccatttc aaccgaggac tacctcgccc
    19921 acctctttct ccattttcag aaaaaccaag accatatcgc cagtctgcta ttttccaaaa
    19981 atgactattt cctccgtcaa ctacagagag agcttgagca ccacgtctat cccatgctgg
    20041 ctgctgattt gaagcaaact cacccaagtc tacctacttc ttacctccaa cattttgtcg
    20101 taactaactt tatcgaaaca ttgacctggt ggctcaaaaa aggtcaagat ttcacagacc
    20161 aggaagttgt ccaattttat ctagaccttc tcattcctaa aaattgaata tagagtaaag
    20221 cttcagttgt cttatttcta ggttactgag ttttttatct tttcaacaac aaaagaggac
    20281 ccgccgatcc tctttttcat actataaatc cttgattatc aactatatct gttttaatcg
    20341 aaatctcaaa acagcacttt caaacatctt ttcctagtta agtaaatcag tattctgctt
    20401 agctgccttg ctccattgat accaaccaac tagactgtta atgagataaa ttagatattt
    20461 cccttgaatt tgcaggcttt ctccccacca gagatagatt gaaaagacat tggtagccgc
    20521 ccagaatatc cactgttcac ggtaaacagc tgtcatgagg atttgcccta ccccattggt
    20581 tgcatctgtg attgaatcac gatagggacg attggcacca atagactgat aaatgaagcc
    20641 aaaggccaac caccaaagca cactaatgga aagatacttt gtccagccct tgccgtccag
    20701 tttacgcgcg acaaactcct gcttttcctt cttaaactgt gcctgataaa tccaaactag
    20761 aagtccaatt ggctgcatga ctgtgaagta aagtgtcgtc agcacctcac cataaaagcc
    20821 tttctgtagg gccaaaataa ggtaaataac agagttaatc aagccaaaaa gataattact
    20881 tgctcgacct tccgatacaa agattacaca gataatccct gtcaagctac aaatcatccc
    20941 aatccagtca acaatacgat gttcgtaaac caactccagc cagagaggaa aacttcctaa
    21001 aaccagcaaa taaatccact gggcaaaact acgatgggca aagaggtcat cccagatagc
    21061 cttcatagtt cctgaaaatc ctaaatcagc catagccgca accatacgac ggtaaccacc
    21121 tgacatttca cctagggttg ttttgatatt ttcaattttc ttttgcaaat aagtatgcat
    21181 catttctcct tttgttttta aagagccgtg tctggataga ctttcggacg caacgctcta
    21241 ttagataatg aactgcctat acacaagatt tctaacctta gtcgacatga gctgaaaccc
    21301 cttatttgtt aagtagttca caaaatatta tacacctatt ttatgaatag tcaactgtct
    21361 ttacagtaaa attttagaaa atcatgaaaa ttttctcttt ctttccattt taagtgacat
    21421 tcagtcattc tcacatcaaa aaagcccaga cgaaattgtc tgagcattct tttatctagt
    21481 cgtttaagga agttgagttc agtatgttta aagtctctgt cccatcattt cttcaacaaa
    21541 ccttgttctt ggagaaactc cttggctact tgctttgctg acttgccttc aacaccgact
    21601 tggtagttga gctggctcat ctggctttct gtaatcttac cagccaatgt attaagaact
    21661 ctttccaact ctgggtgttt cttgagaaga gcttctttca tgagtggagc cccttgataa
    21721 ggtgggaaga gttgcttgtc atcttccaag acctgtaaat cataacgctc caattccgca
    21781 tcagtcgaat aggcgtccgt aatttgaata tcacctgact gaatagcctg atagcgaagg
    21841 gctggctcca tggtcgctac attaagattg agaccataca ttgattgcaa gcccttattt
    21901 ccatcttcac ggtcgttaaa ctcgagtgta aaacctgcct tcaactgccc ttccactttt
    21961 ttcaagtctg aaatggtctt caagccatat tcttgagcaa tctttttcga aacagctaca
    22021 gcataggtgt tttgataaga catgggtttg agataggcta gatgatcctg cttagcaatg
    22081 ccatcacgcg ccacctgata aacctgttct ggttcatgac tcaccttggg tgatggttga
    22141 agcaaacttt cagtcaccgt accagtaaat tcaggataga tgtcaatatc gccttttttc
    22201 agagcttcat aaaggaagct tgtcttccca aaattcggtt taacagtcgc agtcatgctg
    22261 gtattttctt caatcagcaa cttatacata ttggccaaaa tttctggttc tggacctatt
    22321 ttcccagcaa taaccaagtt ttccttctct ttttgaacca aaagagctgg actataagac
    22381 agacccagta ataaagccac caaggcaaaa cctgagaaaa tcgtccgtaa ttttgctttt
    22441 tccattactt ttagtaggaa gttaaaggcg atagcaagca ctgcagaaga aagtgcccca
    22501 atcaaaatca aactggcatt attacggtca attcccaaca ggataaagga acccagtccc
    22561 cctgctccaa tcaaggccgc caaggttgcc gtaccgataa tcaaaacagc tgccgtccga
    22621 atcccagaca tgataacagg catggcgagt ggaatttcaa atttcttgag acgttcccat
    22681 ctggtcatcc caaaggcaat cccagcctct tgcaggttcg gatcaattcc cttcagccca
    22741 gtgatagtat tttgcaaaat agggaaaatc gcataaatca ctagagctgt caaagccggc
    22801 aaggtcccaa ttcccatcaa agggataaag agccccaaca aggccagaga cgggatggtc
    22861 tggaaaatac cggcaatctg caagacccag tcggccagct tctcatgata gcgaagaaaa
    22921 acagccaagg gaatcgcaag caaaatagct agtaacaagg tcaaaagtga caactgcaaa
    22981 tgttgagata gagctgtcaa ccaatcacta aaacgatcct gaaaagttgc aattaaatta
    23041 gtcatgaaca ctacctccaa acaagtctgc tacaaagtct gttgcaggca cttttaaaat
    23101 tgtctcggga ttcgctacct ggcgaatttc tccatcctgc aagacagcaa tacggtccgc
    23161 caacttcaag gcttcatccg tatcatgggt tacaaaaatc gttgtcatcc caaactcttt
    23221 atgcaattct tttgtcagaa cctgcaactg ttttctcgaa atagcatcca aggccgaaaa
    23281 gggttcatcc atgaggaaaa tcttgggctg accaatcata gctcggacaa taccgacccg
    23341 ttgctgttct ccaccagata attcactagg taagcgatgc ccatactcgg ctactggtaa
    23401 accaacctta gccaaaagct cttctgtttt cttcgtaatt tcttccttgc tccacccctt
    23461 catttcagga atgagagcaa tattttccgc aactgttaga tttggaaaaa gagcaatagc
    23521 ctgtaaaaca taaccagtag aaagacgaag ttcacgctca tcatagtctt tgatgcgctt
    23581 cccatccata taaatatttc catcagttgg ttccaaaaga cggttaatca tcttgagcat
    23641 ggtcgtctta cctgatccag aaggccctac taaaaccata aattccccat cctcaatctg
    23701 taagttgaca tctctcaaga catccttttc tgtgtagcgc agtgctacat ttttgtattc
    23761 aatcattctt tgtcctcaat ttaaaacttc cctcgattgg tcaagtcttc taccttaggc
    23821 atgacttcct tattatccca atgctccaca attttcccgt tctctaaacg gaagatatcg
    23881 tactgggcat aagcaacgcc atcaatctga gtctgaccat agctaaccac atagtttcct
    23941 tgtcctaaga gttggaaaac aaagtcaaaa gtgacactat attcagccac atagttttta
    24001 taagcagcac ttccttgtcc aatctcatga ttatgctgaa tcaaatcgtc tgccacataa
    24061 tcactccact gctctagctc cccattttgg aaaatttctg tcaagaaacg gcgaaccagc
    24121 tttttatttt ctgctttctt atccaaatcc ttgatttcaa aatctccaaa aatttgatct
    24181 agttgatcat tttcaggtgt tcgatagtag tcaatgacat cccaatgctc aacaatacaa
    24241 ccattctcat ccgcacggaa agtatccgtc gtcacccatt gagcttctcc accattcaga
    24301 tattgatgaa catgaacaaa gaccagattg ccatcctcaa tggtgcggac aatcttaatc
    24361 tgacgctctg gatgacgctc aaagaaatct gcaaagaagg ctgcaaatcc ttctttcccg
    24421 tcaggaacac ctgtcgaatg ttggatatag gtatccccta cagactgggc ttgagcctca
    24481 gcaactcgtc cgtcttgaat ggcatggatg tataggttgt gagcattttt cacttgttgt
    24541 gacatattct aaacctcatt ttccttctct ttcagatttg ccaaaattct ttcttgaaaa
    24601 ccttcaaatt ggtgaatttc ttcctctgaa aatcctttgt aaaagatagt atccaatttc
    24661 tgactgacac gatgccccac ttctttctgg aacttgccta actccgttaa aactaaatac
    24721 ttcttacgct tgtcttttcc acacggacta acaattacaa gcttttgttc ctctagcttt
    24781 tttatcatag tcgtcagcgt attattcgca agcccagtcg caagcgcgat atctgtcgca
    24841 gttgcgcagc cagtttcact attccataaa accgctaaaa tcttgccctg ttcaccccta
    24901 taaagagcct caggatcttg actcagtaac ttttgaaaaa tccgcccatt caacaaacga
    24961 atatgatggg ctagcaaatg actatctttc ataacacctc caatttattt cgatatcgaa
    25021 atgaataaaa taattgtaac actcatcgtt ctaactgtca actatttcga tttggaaata
    25081 atttttgata attattcaca ccaccatact ccggctcaac taacttttaa cgagagtttc
    25141 taaattcctt cgtcctccag tctacaaaag ccttccatcc gtactatcct attttttatg
    25201 aggggacaca tttttcctat cagatcattt attttaaaga tagaagtaaa tcataattgc
    25261 ttccatctgt tcttttatag tatattgaag ttggactagg gcactgtatc ttctaaaaca
    25321 ttgatagaaa gcgatttgaa tttcccaatc aatttgttcg tatttatagc attttgaaac
    25381 tagaatagga cgccatgact gctaaaagat ttctataaat tcatttgatt tccctaatca
    25441 atttgttcat atcttatttc attccgctat aatttcacct taccctatct ttttcgtagc
    25501 acccttcaaa cagcctatcc cctaccgttt gacgattcct cacttcactc cacttccatt
    25561 acagaagttt cttcactact atggtctcgg ctgacttctc atgattcctt gttactacta
    25621 tttgaacgct cacgagatag gtcttataaa aaatgctttg atccacaatg gaatcaaagc
    25681 attttaaaaa gttcctcata cataagcgca gaagtcgcag ttcctctgta cttggcttct
    25741 tctcttttga caaagcgagc caagttgagc aactcaggtg ctggatgttt gggatttagg
    25801 agcaattcac gattgaccag gcttgagaga cgaactgcct gcaattgctc atttgtagta
    25861 ggcagttttt tagtagtctc taggagagca gcaactaaat cttcactcaa atcatgtcga
    25921 gcatgattgt aaagatcttt tataaggctt tctaggtttg gttctaccat ccctaccacc
    25981 tcccttatgg tttaataatg tttaatcaaa tcaaccgttg aacgatccaa tttcttcacc
    26041 aaggcttgta agaaagcttg cgcttctagg aagtcatcca ttgcatagag ggtttggtga
    26101 gaatggatat aacgagcgca gacaccgata gttgttgatg ggacaccacc atttttcaga
    26161 tgagctgcac ctgcatctgt tccgccttta ccacagtagt attggtactt gataccagct
    26221 tcttcagccg ttgtcaaaag gaaatccttc atccctggga gaagcaagtg acctggatca
    26281 tagaaacgaa tcaaggttcc atctccaatc ttgccttgac caccgtagac atcacctgct
    26341 ggtgagcaat caactgcgag gaagacttct gggtcaaact tggttgtaga ggtatgagcg
    26401 ccacgcagac caacttcttc ttggacgtta gaacccagat agagttcatt gccgagtttt
    26461 tgacctgata aagcttcagc tagctcgctt accatgagga caccgtagcg gttatcccaa
    26521 gcttttgaga tgatattttt ttcattggct gtcaaaattg cagaactatc tggtacgatg
    26581 gtatcaccag gacggatgcc aaaactttct gcctcagcct tgtccgcaaa accaccatca
    26641 aaaacgatat cggcaatggc tggcatggtt ggtcccccct ttccacgagt caaatgcgga
    26701 ggaacagaac ctgaaatcac aggaatttca tgaccatcac gagtcaagag tttgaaacgt
    26761 tggctgctaa ccaccatggg gttccagcca ccgatttcta cgacacggaa ggtaccatct
    26821 ggcttgattt cgctgaccat aaaaccaact tcgtccatat gagaagcgac caagacgcgc
    26881 ggtgcatcca cagcttctga atgtttgata ccaaaaatac cacccaagcc atctgtcacc
    26941 acttcatcca catgcggtgt caacttttca cgaagataag cacggacagg cgcttcatga
    27001 cctgagactg cagcaagttc tgttacttct ttaatttttg aaaataatgt tgtcatttca
    27061 gttccttctt tctttcatcc attttaccac tttttatagg agaaggatag tgggaaggtg
    27121 gatttctaag ttagtatctt agtcctgctc tatcttagaa aaggatagta ttctcttgca
    27181 tgtagtgcaa aatctagtaa acattccaaa attaactcga atatttattt ccaaacaaaa
    27241 aaacaataca ccatcaaagt tgtttggatt tttcatgaaa tttacagaaa atagttgact
    27301 tccctttctt ctttctttaa atatatagtt ggttgagttt ggaatagtac gctgtagctg
    27361 ctgaaacatt tctagaaatt aatttgactt tcctaataga gttgttcata tcttatttca
    27421 atttactata gtacaaaact agaaaaggaa aaaatcatga ccaaaacaat tcttgttaca
    27481 ggtgggacta gctacattgg ctcccatacc gttaaagctc ttttaaatgc tggctataag
    27541 gtgcatgtcc tcgataatct ctccacagga aatcgagcgg ctgtggatag tcgtgctagc
    27601 tttaaagaac tggatgttta tgatgctagc gccttaaagg cttacttaga agaaaatcag
    27661 attgatgctg tcctccattg tgcaggtgaa attgttgtga gcgaaagtat tgaaaatcca
    27721 agtaaatact tcactgccaa cgttgctggt atgaaccaag ttctcaaagt cttatctgaa
    27781 gttggcattc aaaaaatcat gttctcttcg actgcttctc tctatggaaa taactgtatt
    27841 gacaagccgg tgactgaaga taccctgctc gaccctgtca acccttatgc agagacaaaa
    27901 ctgatgggcg aacgaatgat ttactggatg gccaatcgct acgactggaa atatgttatt
    27961 ttccgttact ttaatgttgc tggggctgaa atggatgctt caaacggtct gcgtgtgaaa
    28021 aatccaactc acatcattcc aaatatcaac aagaccgcat tgggacaaaa tgatagctta
    28081 aaaatatttg gagatgacta cgatacacgt gacggttcat gtattcgaga ttacatttat
    28141 gtcttggacc ttgcataggc tcatgttaaa ggaatgaact acctatttca agaagacagt
    28201 tcttctcaaa tctttaactt aggaactaaa aaaggctata ctatcaaaga aatctttaaa
    28261 actgctgaag aattactaaa ttaaaaaatc ccacacgaga ttgttgctcg acgtgctagt
    28321 gacccagcca gtgtcctagc agatgcttca aaagctaaac aatatcttga ttggacggct
    28381 agctactccc tcgaagatat tattttatca gattaaggta aaaacatttt agaataggat
    28441 aacaaaggag aaagtcttgt tgctctctcc ttgttgtatt tattaaattg tcagaaattt
    28501 tattgacaga ttagaaaaat aatgttacat ttatatccgc agatatcttt cgataccaaa
    28561 tctacatgaa aggacggggt atgaaacttt ctcattattt aattggctta cttctactcc
    28621 tagtctttct ctctattagc attgggacca atgatttttc atggggaaag ctatttgatt
    28681 tcgaccagca gacctggctc ctctttcaag agtcccgtct cccaagaact atcagtattc
    28741 tcctgactgc ctctagtatg agtatggcag gccttctcat gcagactatt acccaaaatc
    28801 agtttgctgc accgagtaca gttggaacga ctgaagccgc caaactggga atggtgctga
    28861 gcctttttgt ctttccatcg gctagtctga cccaaaagat gctcttcgct tttgtttcat
    28921 ccatcgtatt caccctcttc ttcctagcct ttatgaccat ttttactgta aaggaaaggt
    28981 ggatgttgcc tctgattggg atcatctata gcgggattat cggctcagtc acagaagtta
    29041 tcgcctatcg tttcaatctg gttcagagta tgactgcctg gacccagggc tccttctcca
    29101 tgattcagac ccatcagtat gagtggctct tcttaggcct catcatcctg ataaccgttt
    29161 ggaaattatc ccaaaccttc accatcatga atctagggaa agaaaccagc gagagtttgg
    29221 ggatttccta ctccctactt gaaaaactgg ccctctttct ggtggcgcta acgacaagcg
    29281 tcaccatgat taccgtgggt ggcctaccat ttctcggagt tatcgttccc aatcttgttc
    29341 gcaagcgcta tggagataat ctaagtcaaa ccaaactcat ggtcgcactg gttggtgcca
    29401 atctagttct ggcttgcgat atcctatccc gagttctgat taggccctat gagttgtctg
    29461 tcagtctctt gctaggaatc atcggtagtc tcgtctttat cctacttctc tggagagggg
    29521 gacgaaaaga tgcagactaa aagcaaacat accaagctct tctggattct cattattctt
    29581 gccatcggag cttgtcttct ctacttttgg cccatcactc acttgtcagc ctttgcttgg
    29641 aagttgcgtt cccaaaagat catcgtttat ctcttggtag ccatcgcgac tgggatttcg
    29701 accattagtt ttcaaaccct gacggaaaat cgcttcctga cgcctagtat tttgggaatt
    29761 gaatccttct acgtcctact acaaacccta ctactggttt ttgaaagcaa gtttcttcaa
    29821 cttggcaaat cccctatctt agaattccta gtcttacttc ttgtccagtc cctcttcttt
    29881 ctcgccttac aaggttactt gaagacactg atgaagcaag acctggtctt catcctgctg
    29941 atctgtctag cgctcagaag tctctttcga aatatcagca ccttccttca agtcctaatg
    30001 gatccaaacg aatacgataa actgcaaaat agtctttttg cctcctttca acatctcaac
    30061 acttccatcc tagccatcgg ttctctgatc atcctcgctt tgacaatctt tttctttcga
    30121 aaagcagtcg ttctagatgt cttgcacctg caaagagaaa cggctcagat attgggactc
    30181 gatgttgaaa aagaacagaa agagctcctc tggggaatcg tgcttttgac ctcaacggcc
    30241 acttccttgg taggacctat ggccttcttc ggctttatgc tggccaacct cacctacctg
    30301 attgtcaaag actatcagca caagttactc tttatagtgg ccattctggt tggatttatt
    30361 agcttaacct tggggcaagc cttgattgaa cgagtctttg cactggaaat tcgtatcagt
    30421 atgatcattg agagtgtggg tggcttctta ttctttatct tactatatag gagggctcgt
    30481 cagtgaaact ggaaaacatt gacaaatcca ttcaaaaaca ggatattttg caaggcattt
    30541 cgcttgaagt cagtcctcaa aaactgacag cctttattgg tccaaatggt gctggaaaat
    30601 cgactctcct ctccatcatg agcagactaa ccaagaaaga tcagggagtt ctcagtatca
    30661 aaggacgtga aatcgagagc tggaattcgc aagaactggc ccaagaacta accatcctaa
    30721 aacagaaaat caattaccaa gccaaattga ctgttgaaga actggtcagt tttggacgtt
    30781 ttccctacag ccgaggtcga cttagatcag aagactggga aaaaatccga gaaactctga
    30841 actatttgga actgaccaac ttaaaagacc gctacatcaa tagcctgtca ggggggcaac
    30901 tccagcgcgt ctttatcgct atggtactgg cccaggatac ggactttatc ttgctggacg
    30961 aaccactcaa caatctcgat atcaagcaaa gcgtcagcat gatgcagatt cttcgacgac
    31021 tggtggagga actcggcaag accattatca tcgtcctcca cgatatcaac atagccagtc
    31081 agtatgcaga tgaaattgtc gccttcaagg acggccaggt ctttagcaag ggaagaaccg
    31141 atcaaatcat gcaggctgac ctgctcagtc aactttatga gattcccatc acgctagctg
    31201 atatcaatga caaaaagatc tgtatctata gctagtaaca taaaagctca agttagagaa
    31261 ccttcagtct cttagtcaat aagatcaaga gactccctaa atcgttatca cattttaaag
    31321 aggagaaatt atgaaaacat cccttaaact ttatttcact gccctagtgg ccagcttctt
    31381 gctcctactt ggtgcatgta gtacaaactc aagcactagt cagacagaga ccagtagctc
    31441 tgctccaaca gaggtaacca ttaaaagttc actggccgag gtcaaacttt ccaaagttcc
    31501 tgaaaagatt gtgacctttg acctcggcgc tgcggatact attcgcgctt taggatttga
    31561 aaaaaatatc gtcggaatgc ctacaaaaac tgttccgact tatctaaaag acctagtggg
    31621 aactgtcaaa aatgttggtt ctatgaaaga acctgattta gaagctatcg ccgcccttga
    31681 gcctgatttg attatcgctt caccacgtac acaaaaattc gtagacaaat tcaaagaaat
    31741 cgccccaacc gttctcttcc aagcaagcaa ggacgactac tggacttcta ccaaggctaa
    31801 tatcgaatcc ttagcaagtg ccttcggcga aactggtaca cagaaagcca aggaagaatt
    31861 ggccaagcta gacaagagca tccaagaagt cgctactaaa aatgaaagct ctgacaaaaa
    31921 agcccttgcg atcctcctta atgaaggaaa aatggcagcc tttggtgcca aatctcgttt
    31981 ctctttcttg taccaaacct tgaaattcaa accaactgat acaaaatttg aagactcacg
    32041 ccacggacaa gaagtcagct ttgaaagtgt caaagaaatc aaccctgaca tcctctttgt
    32101 catcaaccgt acccttgcca tcggtgggga caactctagc aacgacggtg tcctagaaaa
    32161 tgcccttatc gctgaaacac ctgctgctaa aaatggtaag attatccaac taacaccaga
    32221 cctctggtat ctaagcggag gcggacttga atcaacaaaa ctcatgattg aagacataca
    32281 aaaagctttg aaataagcca cttcaaactc aatttaatac tcaatgaaaa tcaaagagca
    32341 aactaggaaa ctagccgcag gttgctcaaa acactgtttt gaggttgcag atgaaagctg
    32401 acgcggtttg aagagatttt cgaagagtat aaaattttga cattctaaaa cgcatcctat
    32461 caagtttact caaacctgat aggatgcgtt tttatcattt tgaaatctat ttaccccacc
    32521 tcattcccct tcttgatttc gtttaaggga aaagtggtct ggaacggggt ccttacctgt
    32581 tttcgaccag ggatgacaac gtaaaatccg agccaagccc atcaataccc ccttaaaacc
    32641 atgtttttca atagcctgaa tcatgtagtt ggaacaagtc agctcaaagc gacaagaggg
    32701 tggaaagact ggtgagataa aacgttggta aaagcgcaca ggcgctatta aaattcgttt
    32761 cattatttct tagttacagc catggtgtgt agttggctga tttctttttt attaagacga
    32821 cgggattctc ctggacggag tcctgtcaag tctaggtgtc cgaaacgagt ccgagacaac
    32881 ttatctactt ggagaccaac agcttcaaac atctttttaa cctgatggtt acgcccttca
    32941 tggatggtca actgcaccac agagcgattt ttgactgggt ccactttgag aatttcataa
    33001 acagctggct tggttttctt accatcaatc tcaagaccac gggtcaaggg gcggagattg
    33061 tccttattgg ccacaccttt aacacgcgcg acataaacct tgtcaatctc attacgaggg
    33121 tgaatcatct cgtctgtaaa gtccccatca ttggtcaaaa tcaagacacc tgatgtatcc
    33181 cagtccaaac gacccacagg gtaaatacgc tctttgacat tgggcaagag gtcgacaacc
    33241 gtcttgcgac ccttatcatc tgtcacactg gaaatcacac cgcgtggttt gttaagcaga
    33301 tagtagacct tttcttcgtt gtagataggt tgaccttcaa cttcgacctt gtcgcctgac
    33361 ttgatagtgg ttgctagttc acgcaccact tggccgttaa ccgtcaccaa gccttgctta
    33421 atcagctctt ctgcttttct cctactggcc acacctgcgt gggcaatata cttattgatt
    33481 ctcatcttct tctatccttt caccaaataa ttggctttct tgggcttgaa tctcaagctc
    33541 atcaatcact ggtaattctt ctaaatggtt tatccccatg taatctagga aataatccgt
    33601 agtcacatag aggttggggc gccccaatac ttcctttttc ccgtcttcct ttatcaggtc
    33661 aaaagcctgc aactttgcca aggctccact cgagttaact ccacggatgg catcaatttc
    33721 tatccgcgta atcggctgtt tgtaggcaat aatggacaag gtctcaaggg cagcccgaga
    33781 caagctctgg ttgataggcg ccttagagta ttccttcaaa atctctgcaa attgaggctt
    33841 ggtcaccaat ctataagcac cacttgtctc aatcaaagcc aaactggaat ctgggtcctt
    33901 ttcatacttc tgggctaatt ttcctaaact ttgctggatg cctgtcggtg gcagagagag
    33961 gagttcagct aactggcgga cccgaatccc atcttcaccc gctacaaaca agagcgcttc
    34021 tatttttgct aaagtactca tctttcctct ctatcaagtc tagctttgag gcacttgact
    34081 ttcttccttc ttttccatga gatagatatc tccaaaactc tcctcttgca cgaggatcag
    34141 ctcctgggtt ttgattaact ctagggttgc caaaaagagg gtgatgacct cttggacatt
    34201 ctgggcttcc ttgaacaaat cctgcaagcg caattgatct cgtccaatca aggactcttt
    34261 cacgataatc atcatgtcct caatcttata ctcatcccgc aagatcgtcg tgtgattttg
    34321 tgcaaactcc tcttttttct tggctaggat atttgaaaaa gccaaaaaga ggtcaatggt
    34381 cgtcttgtca tgcacaagct ccgcatcttc gtaaatcaac tctgtcggcg ctttggaata
    34441 atactgggcc cgttcttggt gcttggcttc caagtgctca cccaagagct tgaacttgcg
    34501 atattcttcg atttgagaga ggaggtcctg ctccaggtca tcccccaagt ctgtcacttc
    34561 tgctaccttc ggaaggagtt tacgactctt aatcagcatg agctgactag ccatgaccat
    34621 gtactcaccc gtcacttcca gacgcatggc ctgcagggtt gagacatagg ctagatactg
    34681 ttcgatgact tccgtaatgg gcacatcgta gatatccatc tggtacttag aaaccagatg
    34741 caagagcaag tccaggggtc cttcaaaatc ttttaattta atatccatta tctatatttt
    34801 tctaaggtca ggactgtttt taatcctaat ttttttgcaa tttcgtacaa atcgaccttg
    34861 ttttctattt gtcttagaat aaactgttca cgtaagactt gagctgataa ggatggaaaa
    34921 cctttctcct tgacaaaaga ttctaactga cgaaaggccc actgacgaga atagggtttc
    34981 tctcctcttt caaaaagata ggtctgcccc atcaagggtt ccaattctga aagcaaggcc
    35041 gtgggaatgg tgacaatcct ctgttgggaa gccttgctga ttcgcagcac ctgaaaatcc
    35101 agattgatgt ccgcaacctt gatggctaaa atctcactgg gcaagagccc catttctagg
    35161 attaagagcg ctagcaagcg gccctctgga tggtcgcttt cctgccaaaa agagtctagg
    35221 tatagaatct ctggcttttc cgtcttcttt tcagcttgtt tggctaattc caagcggtaa
    35281 aagctgtcca cctctccttt ttgatagaga aagtatagaa attggttaca ggccgaaatc
    35341 tttcgcttct gggcgctgat ttttagattg gctagctggg cttggtaaat cttgagactg
    35401 gtctcagaaa tccgctcacc taccatgtct aaaaattgct ccaaatcata cttataggac
    35461 tgcttggaat tgacagacaa gccctgcttt tcctctaaaa aggctgaaat cctgtctctc
    35521 attggcatcg aatctcatat tccttactaa agtcatgcaa gagggcatta acggccttga
    35581 ggatggactt gcgcgtaata atcccttgga aaataccctc tgcatccacg accggtaaga
    35641 aggactcatc tactagcttg tgcaagacct ccgtaatggt gaaatcaggc gaaacaaccg
    35701 ctacgtccgt ttttgtcata tgaacgatat ccgtatccgc catgatttct tggctcaagt
    35761 catgctccat ctgataagcc ataatatctc tgagtccaat cgtcccaaca aactgttttt
    35821 catctgtcac aacgggaaca cgggtatagg tcatctgact gagcaagagg gtcgcatgat
    35881 ccgcattgtg ggtatcaatc aacacagcta gatttttagc aggggtcaaa aaagtttcct
    35941 cctgccccaa caagaaagtc tcaaactcct tggcaatcat cggctaaact ccttggacaa
    36001 acctggatac acctcgtgat ctcgtgtcaa aaagtccact ttgaagtaac tatcatcaat
    36061 ctccacacga gcatagagac attctctgat ggtacctcgt ggttgactga tagaacctgg
    36121 atttagaaag aggatcttgc cttccaacca agcacttggc acatgcaagt gaccatagag
    36181 gcagatagcg gcctcttcct cctgagccca gtagtccaac ttttgaaagt tgaaattgat
    36241 gtcaaacaag tgaccatgag tttggataat cttggtcgaa ccaagctcag tcaccagacg
    36301 ttctgggtag ccggcgtaga agtccatgtt ccctttaaca acgcggatgc cctcccaaag
    36361 tggagaatcc ggacgtagtt cagaatcgcc gttatgaaaa acggcatcga ctttgcccac
    36421 atagcgatca cggacttctt ccacaatcaa gctatcgcca tgggaatcgc tcattacaat
    36481 gatggtttgc tttgccatga tggaaatacc tccaaaagtt tcttaacggc taaggcacgg
    36541 tgagattgac tatttttttc ttctagggtt aattcagctg atgactcacc tgtctctcct
    36601 acaaggaaga ggggatcata gccaaagcca ttttcaccct taggttcaaa gttaatataa
    36661 cctgaccagt ctgcttcaac aactaaactt tccttatttg ggctggctac gactagggtt
    36721 gtgtggaact gagccgagcg gtccttgagt tcaaagacca tggccaattc gtgcaagagt
    36781 ttggcattat tttcacggtc agttgctccc acacctgcga aacgagctga ccagacgcct
    36841 ggtaagccac caaggacatc gactttgaga ccagaatcat ctgccaaaac catcttaccc
    36901 gttaattgag aaatggtttc tgccttaagg cgggcatttt cttcaaaggt catacctgtt
    36961 tctgctactt caggcaggtc agggtagtca ttaagatttt ccacatcgta gcctaactta
    37021 tcaaagatag ctcggaattc cttggttttg ccttcgttac gagtcgcaat caacaaggtt
    37081 tctctgacct tgcttgtctc aaagaaatca tgaacattga ccccttcttt aggcaattct
    37141 acatacaaga gttgcccatt ttcaaccaag agcagggccc catgacgttg aataacttcc
    37201 aagtttcgtc ccatttcttg attaagcata tctaccacaa aggagaacaa acggtaggta
    37261 gaaccatagc gtaaaacagt aactgaaagc gggaaaccat actcttcatc tccaaatatt
    37321 ttggagaatt caaacagagg aaaatctgtc ttatagtcgc tcaaactgtt aacgccacca
    37381 aaaatactat aagacccaac ataccagtcc tggtcatcct tatattcata aattttattt
    37441 gtcataattc tacatgctcc acatgaatct ctttttccag ccattcttca ccaatttgtg
    37501 caaaactttg gctactggct gttgtgtaaa aacggtgatg gagtggtcca gcatcgcgac
    37561 cacgattgat ttcaaaataa ttgagtaaga ctgagatatc ccgtacgcac tctgccccac
    37621 tatcgatgag ctgaaccttt ggccccatca cattttggat aatagggcga aggagtggat
    37681 aatgagtaca gcccaaaatc aggctatcca cctttccaac caagggacgc agggtttcat
    37741 agaccacctt cttggtaaca ctggttgaca gggcacctga ctcaaccaag ggagcaaact
    37801 tgggacaggc caagctctcc acctgtaagt cgggatccag atcatggatt ttctgacggt
    37861 atatgtctga ttgtaccgtc atgggcgttc caatcactcc gattttccca ccttgactgg
    37921 acttgatggc tgccgaagct cctggcaaaa ttacacccaa gacaggaata tctagttgag
    37981 ccttgatttc ttcccagacg accgcagtcg cagtgttaca agcaatgaca atcattttga
    38041 catccttggt caagagaaag ttgaccagct gccaagtata ttcacgaatt tgctcagcag
    38101 gacgggggcc atagggcgcc cgcgccgaat ctccaatata gacgatttct tcatggggaa
    38161 gctggcgcat gagctcgcgc acaacggtca agcccccgac acccgaatcc aaaaaaccaa
    38221 ttggtcgatt atccatacag tcttctttct agtctttttt ctgattgatc gttcattatg
    38281 attccttgta ctgaacgaac tgaccgacag cttgatagaa tgtcaactac ctctctctta
    38341 atcataatca aatatcaata gaatgcaagg aaaaagtctc atccctaaac cataaccaac
    38401 atagtgagaa gtctggaact ttcatcccag acttttcctt atttattttt tctttttatt
    38461 gttagcaagg gctgcctttt gttggcggat gatttggtgg taaacttgtt gtaccttagc
    38521 ttcgcttggt ttttgaccat ttgcactcaa aagagtacga actgcttcag cattcaaacg
    38581 tgggttgtca gcaaattctt tttcgatttg cttacgaacc aagtacattc ctccaagagc
    38641 tcctcctaga aaagctagca caatcaatac aattgctaaa agtaaatcca tcatttttct
    38701 cctgtttctt tttgagtccc tttcattata ccacaaacta gccaccctga ccagtttgtt
    38761 ctacactttc aaggatggaa tagacagcaa aaagaaccct gcttttctga aaaagaggtc
    38821 tccttgctaa gtttatactc aatgaaaatc aaagagcaaa ctaggaagct agccacaggt
    38881 tgctcaaagc accgctttga ggttgcagat aaaactgaca tggtttgaag agattttcga
    38941 agagtattaa tttacataaa tagccagtgt ttgatagggt ttgagtagaa ttttctcaga
    39001 cacttctgca tcttcatagt ttgaaatcaa aatctgtcca ttttggtaga ctgctggcaa
    39061 gtcgatttcc acttctttag cataaaagtt attgagcact agtaactttt gatcctcaaa
    39121 ctggcgttca aaagcgtaga cttgtttgct atcttcaaag gctggtttgt aacttccttc
    39181 tgaaatgatt ggcatttcct tacgcagtcg aatcaagtct tgatagaagg taaaaatcgg
    39241 accctggatt tcattttcta cattgatgtc tttataggat ttaccagctt tcaaccaagg
    39301 agtgcctgtt gaaaatcctg cattttccga agcatcccac tgcatgggaa tgcgtgaatt
    39361 atcacgcgac ttagcttgaa taatctggaa ggcttcttgc tgactctttc cttcttctaa
    39421 gagcatctga taggcattaa gcgattcgac atccacataa tcagccatag aatcatagtc
    39481 tgggtcaatc atcccgattt cctcacccat gtagatataa ggtgtcccac gtgacaggtg
    39541 aatgctggcc gctagcatgg tggctccttc cttgcggaag ttttgaatat cgacaaaacg
    39601 gttcaaggca cgtggttgat cgtgattgtt ccaaaagagg gcactccaac cgtctttatc
    39661 actcatttcc ttaccccaac tatggtaaag actcttcaac tcttcaaaat caaagggagc
    39721 caaggtccac ttttgtccat ccttatagtc caccttgagg tgatgaaaat taaaggtcat
    39781 ggataattcc tgacgatcag gcgacgaata gaggacacag ttttccatgg tggtagaaga
    39841 catttcccca actgtcataa agctatcgtc agatccaaaa gtggcttggt tcatcatacg
    39901 caaatagtta tgaacgatgg gtttgtctgt ataagctggc ttcccttcat tttcaggaca
    39961 gtccactgaa acctcgtcct taccgatcaa attgatcaca tcaaatcgga aacctttgac
    40021 acccttgtcg cgccagaaat taacaacctt gaaaagctcc ttacggacat tggaattgcg
    40081 ccagttaagg tcagcctggg tctcatcaaa taggtgaaga tagtatttcc cagtatcccc
    40141 gaaaggcgcc catgcagaac caccaaactt agactgccaa tctgttggtt ggtcttggat
    40201 gaagaaaaag tcttgataat acttatcacc agctagggct ttctgaaacc attcatgctc
    40261 tgtcgaacaa tgattaagta ccatgtccag cataaagtca atcttgtgct ctttaccgac
    40321 acacaccatt tcctcaaaat cagccatatc accaaaaaga ggatccactg ccatataatc
    40381 tgaaatatcg taaccattat cccgttgagg gcttggatag aatggattga gccagaccat
    40441 atccacgcct agtttggcta aatagggaat tttttcgata atcccacgga aatccccaat
    40501 accgttttca gtggtgtctt tgtaagattt tggatagatt tgatagacta cttttccttt
    40561 atcaagtgtc atctgtttct ccttttctga taaaagggag gaagcagtct tccgtcccta
    40621 tttgtgctat ttcaattata ctcaatgaaa atcaaagagc aaactaggaa gctagccaca
    40681 ggttgctcaa aacactgttt tgaggttgca gatagagctg acgtggtttg aagagatttt
    40741 cgaagagtat tagattcgtg tagcgaccat gagagatgct ccagcttgaa tcgttgtcgg
    40801 ataagttccg ggaatagtcg ctgtataagc atcttggttg gtgatgataa caggagtttc
    40861 tgtcaccaga cctgcagcct taatgacatc catatcaaaa cgaatcagtt gctgaccaac
    40921 tgtaacgtga tctccttgga ctacaagact ttcaaaacct ttgccatcaa gacctactgt
    40981 atccataccg atgtggatga gcaattcaac tccctcgtca gagacaatgc cgatggcatg
    41041 cttggtaggg aaaagaaccg tcactgtccc attaactgga gaggtcaact caccttggct
    41101 tggttcaatg accagacctt gccccatgac acctgatgca aaaataggat ccgtcgcttg
    41161 actcaattct ttcacttggc cagttagtgg gctgataatt tctaccgaag taagttctac
    41221 tggttcatgg ttcacaaatt ctgcttcttc ttgagcaacg aattctgcct gcaagttcgt
    41281 atcgccctct gtttttgtaa agagaccagc cttgcggaag aagaaagtca agagcattgg
    41341 aacaacaatc gcaactagca tagttcctgc aaatggcagc atgtattgag gttgaataga
    41401 gagaatacct ggcaaaccac cgataccaat agaagccgca gttacattaa aagtaacgga
    41461 taacatgcct gcaagggctg aaccagtcat cccagcaaca aatggataaa tatattttac
    41521 gttaacccca aaaagagctg gttctgtaac accgagatag gctgaaatgg ttgcaggaag
    41581 tgaaacctga gcctcacgct catcatggcg atgcatgaaa taataggcaa acacggctga
    41641 gccttgagca atattagaaa gagcaatcat tggccatagg gcagtgccac cagcatccgc
    41701 aatcaattgt gtatcaatgg cattggtcat atggtgcaga cctgtgatga caaatggagc
    41761 gtagagggca ccaaaaattg caccgaagag ccctttaact ggaccagtta aacctgccaa
    41821 gacaactgat gaaagtcctt gtccaattgt ccaaccgatt ggtcccaaaa cagtatgagc
    41881 caaaatcaag gctggaatca atgacaagaa aggtacaaaa atcatagaaa tgacttctgg
    41941 gatatgcttg cgccagaaga tttcaagata agacagactc aaacctgcaa gcaaggctgg
    42001 gataacttgg gcttggtaac cgatacgatt aacagtaaaa tagccaaaat tccaaaccca
    42061 gtttgccgcg atatcagctg ctggcgttga agcaaccgca taggcattga gcaactgagg
    42121 cgataccaaa cagattccga gaacaattcc caaaatttgg ctggttccca tcttacgaga
    42181 aacagaccaa gtaatcccta ctggtaagaa ctggaagata gcttcaccag gcaaccagag
    42241 gaagtgattg acacctgccc aaaactgaga ggattctgtg atggtcttgc catccaacat
    42301 cgaccaatgg acaccttcca agacattacg gaaaccgagg atcaatcctc cgactatcaa
    42361 ggctggaata atcggagtaa aaatctccgc cagagtggtc ataacacgtt ggaccacgtt
    42421 ttgattactc ttagctgcag acttggctgc ttctttggaa acaccctcaa tacctgaaac
    42481 ggctgtaaaa tcattataaa agatgggcac gtcatttcca atgattacct gaaattgacc
    42541 tgcatttgta aaagttcctt taacagctgg aattgactcg atagctttaa cattagcctt
    42601 cttatcatct cctaaaacaa agcgcatccg tgtcgcacag tgagttacgg cagtcacatt
    42661 ttctttgcct ccaattgcct gaagcagatc tttggcttct tgttcaaatt ttcccatttg
    42721 attctcctta tacttgtcaa agcaagtttg atttgataag tatattgtaa acgataccaa
    42781 acttgtctgc aacttgtttg gtataaaaag ggtgtttttt tgtaaatttt ctagcacttg
    42841 tatggtaaaa tagatacagg tgttcattaa actagactaa aaacctatta aagcaggcaa
    42901 aatgaagaaa taccaacaat tatttaagca aatccaagaa accattcaaa acgagactta
    42961 cgctgtcgga gatttccttc ctagcgagca cgaccttatg gagcaatatc aagtgagtcg
    43021 tgataccgtc cgaaaggccc tgtctctcct ccaagaggaa g
//
LOCUS       DAVKLX010000011        42318 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKLX010000011 DAVKLX010000000
VERSION     DAVKLX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42318
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.011"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(47..529)
                     /locus_tag="VW325_000686"
     CDS             complement(47..529)
                     /locus_tag="VW325_000686"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9734126.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(624..2108)
                     /locus_tag="VW325_000687"
     CDS             complement(624..2108)
                     /locus_tag="VW325_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9734127.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            2261..3871
                     /locus_tag="VW325_000688"
     CDS             2261..3871
                     /locus_tag="VW325_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9734128.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGAKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKSVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVEMTD"
     gene            complement(4015..5412)
                     /locus_tag="VW325_000689"
                     /pseudo
     CDS             complement(4015..5412)
                     /locus_tag="VW325_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            5588..7033
                     /locus_tag="VW325_000690"
     CDS             5588..7033
                     /locus_tag="VW325_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9734129.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLMSVTAPTGTDNENIQKLLSDIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEHPDYASKIKKIYTKGFTKKVEAP
                     KTSKDQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVYNDQ
                     DFTSLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDHDRGRNQQKVIAAILQKLTSS
                     EALKNYSRIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGMGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDVLEGR"
     gene            7035..7766
                     /gene="cps4B"
                     /locus_tag="VW325_000691"
     CDS             7035..7766
                     /gene="cps4B"
                     /locus_tag="VW325_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEU9734130.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTSDVLDKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLERDLVHIIASDMHNVDSRPPHMAEAYDLVS
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            7775..8467
                     /gene="cpsC"
                     /locus_tag="VW325_000692"
     CDS             7775..8467
                     /gene="cpsC"
                     /locus_tag="VW325_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU9734131.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKHKLIILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEKVATNLKLD
                     IPAKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIIAVTRVSDVT
                     TLEEARPAMTPSSPNVRRNTLVGFLGGAAVTVITVLLIELLDTRVKRPEDVEDVLQMP
                     LLGVVPDFDKMK"
     gene            8477..9166
                     /locus_tag="VW325_000693"
     CDS             8477..9166
                     /locus_tag="VW325_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEU9734132.1"
                     /translation="MPTLEISQAKLELVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     AGEGKTTTSTNIAWAFARAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIVVDTAPVGVVI
                     DAAIIMQKCDASILVTKAGETKRRDLQKAKEQLEQTGKSCLGVVLNKLDTSVEKYGSY
                     GSYGNYRKQKK"
     gene            9181..10542
                     /locus_tag="VW325_000694"
     CDS             9181..10542
                     /locus_tag="VW325_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9734133.1"
                     /translation="MSEKLAKPSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYVLNPFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESDDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRVIDIVGSLVGLILCG
                     LVSIVLVPLIRKDGGSAFFAQTRIGKNGRHFTFYKFRSMCVDAEEKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            10548..11291
                     /locus_tag="VW325_000695"
     CDS             10548..11291
                     /locus_tag="VW325_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU9734134.1"
                     /translation="MNERIQILGITIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCYEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMKHLLELSNEKG
                     YSVYFFGAKEDVLQDMLKIFEERYPALNVVGYRNGYFSPEDEKHIQEDIKEKKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQNAHLEWLFRVAN
                     EPKRLFKRYFVGNVSFIRKVLRAKRGYKL"
     gene            11288..12112
                     /locus_tag="VW325_000696"
     CDS             11288..12112
                     /locus_tag="VW325_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEU9734135.1"
                     /translation="MNQTEIVKLIQKVELDAIKEFQKICKENNIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREGYDKLPGIFKDRIIAGKYQVLAYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSFLRKLYFGKVYWYRFLASLGTTYVGDH
                     VDMHSAKQKLIIGFFKKLGFAKLFPQNYVYRRLDNLYKKYGWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDAFFKVPTEYDRYLKRLYGENYLHEEPSDDEKKSHLGGQ"
     gene            12114..12992
                     /locus_tag="VW325_000697"
     CDS             12114..12992
                     /locus_tag="VW325_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9734136.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNSNTKQIVIIDNFSNNGTG
                     EKLQELYESDSEIDVLINHENAGFARGNNVAYQFAKEKYKPDFMVIMNNDIEIETEEF
                     EKIVTDIYRKEKFHLLGPDIFSTTYQLHQNPKRLTHYTYEEVKALNEKFKKGSQVSLA
                     LKIKCWLKSSKVLRTAIYQNRRKKKSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIKVLHHQNVATNQVYTNLVEKTLFSNKCNF
                     ESTSYFLKLMKENEGV"
     gene            12993..14327
                     /locus_tag="VW325_000698"
     CDS             12993..14327
                     /locus_tag="VW325_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEU9734137.1"
                     /translation="MTYLFLLCLTLFLLTFFYFFAFNQDLIAPPVVMSVMFLISSVFA
                     LINVQNWNIEYSGLAYLLIISGIIVFSMPLLALNSPILNTKIKVTDRLIDIQFWKIAL
                     TIIVDLIILYLYRREIHNLALSHGYTGSNFQWFFRNATSYEGELTVRTSIRVLIRIID
                     VSAYIFGYTFINNFFIYSHKRSKDLLLLVPFLIFISKTLLSGGRLDIIKILIAYVVMA
                     YIQQKRKVGWDKVISHKYMRLGFVGLIAGIPTFYYSLFLSGRSTTRTVFESISTYLGG
                     SIQHFNQYIQNPIGVAEVFGDESFVAIMNILGNLGFVNYNSTVHLEFRQLGITMGNVY
                     TFFRRPWHDFGLVGMYIFSFVVGVFFAIFYLKLRKSRAGFKLDIHTIIYSYFFYWIFL
                     SSIEQYSFTTISLFTLVFIVLVYLMAFFYWNLDFHRGKLVIKLSDTSIKSEN"
     gene            14355..15779
                     /locus_tag="VW325_000699"
     CDS             14355..15779
                     /locus_tag="VW325_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734138.1"
                     /translation="MNSKIKNILTNFSYVISSNLLTVLTSSLVVLIFPKLMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYLKYGGLEYNNLDRKQFYSQMILFSSFLMIISLLLF
                     GLNVLAVTDSNARYIYNMTIISMIITNLRTLYVYVLQMTNRLKDSSIILLSDRVLYVL
                     LLFLFIVFGWHEYKVMILADILGRSFSLILSFWICKDIVFQPLSKFIFNIKESFDNIR
                     VGINLMLSNIASSLIIGIVRLGIQWNWNIETFGKVSLTLSVSNLLMTFINAIGLVVFP
                     LLRRTKAENLPKIYSNLRNVLMLIMFAILLIYYPLKIVLDLWLPAYQDALIFMTLIFP
                     MSVYEGKMALVINTYLKALRMERDILRVNALVMLISMGVTLVTTYLLNSLELTVVSIV
                     VLLALRSIIAELILSKKLDVSVKKDIVLEFLLTLVFISSSWYLPIGLAVIVYTIAYGL
                     YLYLKHEDIKTYLAYFKASKKTSN"
     gene            15844..16932
                     /gene="wecB"
                     /locus_tag="VW325_000700"
     CDS             15844..16932
                     /gene="wecB"
                     /locus_tag="VW325_000700"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU9734139.1"
                     /translation="MKKIMLVFGTRPEAIKMCPLVNELKKHEDMETIVCVTGQHKEMV
                     SPVLDLFGVVPDYDLEIMKANQTLFSITTSILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIATYHFAPTELAKENLLKE
                     GRENVYVTGNTVIDALTTTVQEDYTHTHLDLNANNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYEDVKVIYPIHKNPLVRETAAEIFGDTERIQIIEPLDVLDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKLLLDDSG
                     EYKKMSQASNPYGNGDASQQIVQILRGI"
     gene            17095..17964
                     /gene="rfbA"
                     /locus_tag="VW325_000701"
     CDS             17095..17964
                     /gene="rfbA"
                     /locus_tag="VW325_000701"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU9734140.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSV
                     ALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEISYRMGYISREDVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            17965..18558
                     /locus_tag="VW325_000702"
     CDS             17965..18558
                     /locus_tag="VW325_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU9734141.1"
                     /translation="MSDNFFGKTLVVRKIDAIPGLLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADDGKVLGSWVDLREG
                     ETFGNVYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPALGIEWENLAEAEVSEADKHHPLLRDVKPLTKDEL"
     gene            18570..19616
                     /gene="rfbB"
                     /locus_tag="VW325_000703"
     CDS             18570..19616
                     /gene="rfbB"
                     /locus_tag="VW325_000703"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU9734142.1"
                     /translation="MTEYKKIIVTGGAGFIGSNFVHYVYNNFPDVHVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGLGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQPAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKETIKWYTDNQDWWKSEKE
                     AVEANYAKTQQVIK"
     gene            complement(19678..20532)
                     /gene="rfbD"
                     /locus_tag="VW325_000704"
     CDS             complement(19678..20532)
                     /gene="rfbD"
                     /locus_tag="VW325_000704"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU9734143.1"
                     /translation="MILITGANGQLGTELRYLLDERNVDYVAVDVTEMDITNSEMVEK
                     VFAEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKASEKYGAILVYISTD
                     YVFDGKKPVGQEWEVDDIPDPQTEYGRTKRMGEELVENLTSQHYIIRTAWVFGNYGKN
                     FVFTMQSLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYVTENQKEYGYYHLSNDAAEDT
                     TWYDFASEILKDSDVEVVPVDSSKFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALKE
                     FYKQEVRK"
     gene            20778..22760
                     /locus_tag="VW325_000705"
     CDS             20778..22760
                     /locus_tag="VW325_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9734144.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKAEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            23060..28363
                     /locus_tag="VW325_000706"
     CDS             23060..28363
                     /locus_tag="VW325_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9734145.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSQVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGSKTEEELLALEKEKPAE
                     EKPKEDKPAVAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKNVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGNYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGTTTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKVTFLVTGAETGD
                     TWVGIYSTGNASNTRSDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMEALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSIVSDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTSLFLASVSLALSALFVVKTKKD"
     gene            complement(28530..30689)
                     /locus_tag="VW325_000707"
     CDS             complement(28530..30689)
                     /locus_tag="VW325_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEU9734146.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(30686..31282)
                     /gene="recU"
                     /locus_tag="VW325_000708"
     CDS             complement(30686..31282)
                     /gene="recU"
                     /locus_tag="VW325_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9734147.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            31348..31875
                     /locus_tag="VW325_000709"
     CDS             31348..31875
                     /locus_tag="VW325_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9734148.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFENHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            31945..32274
                     /gene="gpsB"
                     /locus_tag="VW325_000710"
     CDS             31945..32274
                     /gene="gpsB"
                     /locus_tag="VW325_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9734149.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            32295..32675
                     /gene="rnpB"
                     /locus_tag="VW325_000711"
     ncRNA           32295..32675
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW325_000711"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            32760..33917
                     /locus_tag="VW325_000712"
     CDS             32760..33917
                     /locus_tag="VW325_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9734150.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDARVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            33930..35324
                     /gene="mapZ"
                     /locus_tag="VW325_000713"
     CDS             33930..35324
                     /gene="mapZ"
                     /locus_tag="VW325_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9734151.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLEDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPQPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            35400..36824
                     /gene="gndA"
                     /locus_tag="VW325_000714"
     CDS             35400..36824
                     /gene="gndA"
                     /locus_tag="VW325_000714"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9734152.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELVNSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            36836..37525
                     /locus_tag="VW325_000715"
     CDS             36836..37525
                     /locus_tag="VW325_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9734153.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            37623..38651
                     /gene="cbpC"
                     /locus_tag="VW325_000716"
     CDS             37623..38651
                     /gene="cbpC"
                     /locus_tag="VW325_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU9734154.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQE
                     KWYYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWI
                     YEEGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGK
                     MLTDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRS
                     SGAMATGWYQDGSTWYYLDPSNGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYN
                     FASSGEWI"
     gene            38669..39667
                     /gene="cbpJ"
                     /locus_tag="VW325_000717"
     CDS             38669..39667
                     /gene="cbpJ"
                     /locus_tag="VW325_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU9734155.1"
                     /translation="MKILKKMMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWRTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYIGGERRAGWINDASAWYYLDPATGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(39785..41017)
                     /locus_tag="VW325_000718"
     CDS             complement(39785..41017)
                     /locus_tag="VW325_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9734156.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(41028..41648)
                     /locus_tag="VW325_000719"
     CDS             complement(41028..41648)
                     /locus_tag="VW325_000719"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734157.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCGIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKVDLFDFGIGSMGVYWCLLNNKFPFDVQT"
     gene            complement(41548..41886)
                     /locus_tag="VW325_000720"
     CDS             complement(41548..41886)
                     /locus_tag="VW325_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
                     /protein_id="HEU9734158.1"
                     /translation="MTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANV
                     TAKKLIENYDTLEEIDFALGKSGVLLSLIKYYQFTNDNTLKTFIHNSIWGNLSLFPTK
                     RYSQRKHFRL"
BASE COUNT        12887 a         7462 c         9323 g        12646 t
ORIGIN      
        1 acgtacggtg gtgtgagagg ggctagagat tatcccctac tcgattttaa atcacatgac
       61 gttcaaaggc atcatctgaa atcccttgtt ccaagatgag ttttgcccat tctttagcag
      121 agaagaggct gtggtccttg tagtttccgc aagattcgat ggttgtccct gggacatctt
      181 cccaagtagt agtttcagcg atttccttga gcgaatcctt gataacagct gcgattttag
      241 cactggtgtg acgtccccac ataatcatgt ggaagcctgt gcggcaacca aatggtgaac
      301 agtcaatcat gccgtcaatg cgggtacgga tgagtttggc taagaggtgc tcgatagtgt
      361 gaaggccggc agtagggata gagtcttcgt ttggttgcac caagcgaata tcataattgg
      421 agatgatgtc tccttttggt cctgtttctt ccccaatcaa gcgaacatag ggtgctttga
      481 caatggtgtg gtcaagttca aaactttcga caataacttc ttttgacatg gtaaatcctt
      541 tcagttttct tctctcatta tatcataaag gttgctcctg agacagagag aaaacctctc
      601 cgaggctgga gaggttgaaa tctttactta cgatataagc ggtcgtattg gtagtatggg
      661 tcaaaggtta cgttgatacc cagtttacga aggacattct tgtcttcatc agtcaagatg
      721 atggttgagt gggcttcgct tcctttgagg ttgccgagtt cttccatagc gcgggcagca
      781 tcaggatttt ctgtagctgt gatagcaagt gcaatcagga tttcatttga atgaaggcgt
      841 ggattgcggc taccgagatg atcgatttta agaccttgga ttggcttaac aacttccggc
      901 tcgattaatt ttacttcttt agcgatgtca gctgattttt tgatggcgtt gatcaaggca
      961 gcggctgtag ggccaaagag ttctgagttc ttaccagtga cgatttcccc atttggcaat
     1021 tcaaaggcta gggctggtcc accagtttct tctgcttttt ggcgcgcaac gacagcaacc
     1081 ttacggtctg caggtgtgat accgaggtcg ttcatgagca actcaatttt cttgacggca
     1141 gcttcgccaa ctttttcagc tttgaagtca agaactgttt gatagtaacg gcggatgatt
     1201 tcttgtttag aagcttcgac agcagcctcg tcatctgtaa tagcgaaacc aaccatgttg
     1261 acacccatat ctgtcggtga agcgtatggt gattttccga gaatacgttc caacatgcgc
     1321 ttgagcactg ggaagatttc gatatcacgg ttgtagttga cagtggtttc tccataggtt
     1381 tgaagatgga aggggtcaat catgttgaca tcatcaaggt cagctgtggc agcttcataa
     1441 gccaagttaa ctggatgatg aaggggaaga ttccaaacag ggaaggtttc aaatttagcg
     1501 tagccagact tgatgccatt gatttggtcg tggtacatat tggacataca cgttgccaat
     1561 tttccagaac cgggtccagg agcggttacg acaatcaagt tgcgactggt tttgatgtag
     1621 tcgtttttgc ccatgccttc tggggaaatg atgtgatcca tatccgtcgg atatcctttg
     1681 attggataat gaagataaga atcaattccg tttttctcaa gttggttgag gaaggcatct
     1741 gcagcgggtt ggccagcgta ttgtgtaatg acaacggaac caacaaaaat ccctaattca
     1801 ttgaatttgt caatcaaacg aagaacttct tggtcataag aaatgcccaa gtcgccacgt
     1861 gctttggaat gttcaatgtt gctagcatta atggcaatca caacctcaac ctgctctttc
     1921 aattcttgca agagcttgat tttgttgtca ggttcataac caggaaggac acgagcagcg
     1981 tggaaatctt ctaacatttt accgccaaac tctaagtaga gcttgccgtc aaattggtta
     2041 atgcgttcca aaatatggtc gcgttgtaaa ttcaaatatt gttcagaact aaaagcttgt
     2101 tttttcattt ttttacctct gacctctatt ataataaaaa attggaagtt aggaaactac
     2161 ggagctaaaa aagaaattaa aaagattaag caaacgcttg cacaaaattt taaaaagtgt
     2221 tatcatatac tatagattat gaaaataatg aggtaaacag atgcaagaaa aatggtggca
     2281 taatgccgta gtctatcaag tctatccaaa gagttttatg gatagtaatg gagatggagt
     2341 tggtgatttg ccaggtatta ccagtaagtt ggactatcta gctaagttag gaatcacagc
     2401 gatttggctt tctcccgttt atgacagccc tatggatgat aatggctacg atattgctga
     2461 ttatcaagcg attgcaacga tttttggaac catggaggat atggatcaac tgattgcgga
     2521 agctaagaag cgtgacattc gtatcatcat ggacttggtg gttaatcata cctcagatga
     2581 acatgcttgg tttgtcgaag cctgtgaaaa tactgatagc cctgagcgag actactatat
     2641 ctggcgcgat gaacccaatg acctagattc tatctttagt gggtctgctt gggaatacga
     2701 tgaaaagtca ggtcaatact atctccactt tttcagcaag aaacagccgg atctcaactg
     2761 ggaaaatgaa aaacttcgcc agaaaattta tgagatgatg aacttctgga ttgataaagg
     2821 tattggtggt ttccgtatgg atgttattga catgattggc aaaattcctg acgagaaggt
     2881 agtcaataat ggtcctatgc tccatcccta tctcaaggaa atgaatcagg cgacctttgg
     2941 agctaaggat ctcttgacag taggggagac ttggggagca acgccagaga ttgccaagct
     3001 ctactctgat ccaaaggggc aagaattgtc tatggtcttc cagtttgaac atatcggtct
     3061 tcagtatcag gaaggtcaac ctaaatggca ctatcaaaaa gagctgaata tcgctaagtt
     3121 aaaagaaatc ttcaacaaat ggcagacaga gttaggagtt gaggacggct ggaattccct
     3181 cttctggaac aaccatgacc tccctcgtat tgtctcaatc tggggaaatg accaagaata
     3241 ccgcgaaaaa tctgccaaag cctttgcaat cttacttcat ctcatgagag gaactcctta
     3301 tatctaccaa ggtgaggaga ttgggatgac caactatccg tttgaaacac tggatcaagt
     3361 agaagatatt gaatccctca actatgcgcg tgaggctctt gaaaaaagtg ttccgattga
     3421 agaaatcatg gacagtatcc gtgttattgg acgtgacaat gcccgtaccc ctatgcaatg
     3481 ggacgagagc aaaaacgctg gtttctcaac aggtcaacct tggttggcgg ttaatccaaa
     3541 ttacgagatg atcaatgtcc aagaagcgct ggcaaatcca gattctattt tctataccta
     3601 tcagaaactg gtccaaattc gcaaggagaa tagctggcta attcgagctg actttgaatt
     3661 gcttgatacg gctgataagg tctttgctta tatacgtaag gatggcgacc gtcgcttcct
     3721 agttgtggct aacttgtcca atgaagagca agacttgaca gtagaaggaa aagtcaaatc
     3781 tgtcttgatt gaaaacaccc tagctcaaga agtctttgaa aaacaaatct tagttccatg
     3841 ggatgctttc tgtgtggaaa tgactgacta gaatgagcaa acctcaagtt tttgaagctt
     3901 gaggtttttt actatagtgg attgaaacta gaatagtgca cctctgcttc taaaacattg
     3961 ttagaaatcg atttgactgt cctgatcgat ttgtcctatt cttatttcat tttgctatac
     4021 ttgtagttga ggagaattaa gctcctcact agtaaactct tcataatcct ttttattttt
     4081 atgaagatat tgtttgaaag atgtgagttt ccacggatgg gtttgtggag ggatatactt
     4141 gcgtctttct ttttttgttc tggttcttgt tcaaagtttt tcgaatagag ttcatgattt
     4201 agtagctcct ttgtgtgata gattttgtca gcgatattga ggtagatgtc tccgtcaaat
     4261 gcttttataa ctaatgcttt cgtctttctg atgaaataga cttcttttcc ttgttcggta
     4321 gggatatagt aacgattttg gaatcggata tggtgtccac tatcgacgac tctctccgcc
     4381 agtctagcca gaatgagatt cctttcagag gggttaggaa cctcctcgaa gacagagagt
     4441 tttgtcttgt ttccaaactg ttcattaaag gtttgaatgt aggaaagcag gaaggtattg
     4501 gcttcttcca aggtatgaat attgttttgt tccagttcga taggcaggcg agattgtagt
     4561 gtctgattga gtctttcgac tctcccttta gcttgaggga tagaggttgt ctcaaggaga
     4621 atccctagtt ggtgacaggc gtatccaaac tgtgtatggg tatcgtcctc cattttctta
     4681 gagttggagg cttgataggt aaagaccgtt ctcttatctg ttttaatttg aaggggaatg
     4741 ccgtgattgg ctaagatttg ttcgaggaca tgatagtaag cattcaagtt ctcttgttta
     4801 tcaaaataag cgcctaggat attgccagaa gcatcatcaa tggctaagtg taagttagat
     4861 gtttgggctc caaactaggc atgaggactg gcatccattt gaatgagttc accagcaaat
     4921 ttctttctgg gtctactagg atgtactttt ttagggtctt caaggaagtt ttcagccgtc
     4981 ggtaagattg gattgtctag gggttgattc aggttcagtt tagcttgttt tcttactctc
     5041 ttctttgtct ttctatggga cttaggcgac aggatatttt tcttatagag tatttttcta
     5101 acagtagtat cagagagctg aattccttct tcttcagcta gcaattcaca gaaatgaagg
     5161 acatttggtt tatatgtttc ataggagagg tattttttta ggagacgttc tttgatttca
     5221 tcagggattg catgttttgg ttttcgattt ctgtttccgt gtctgaaggc ttctttgtcc
     5281 tttcagttga taggctagta gcagacgatt gatttgtctt tcagaaagat tgagctcgac
     5341 acaggctcgt ttctttgttt tctttccttg ggctatagct tttatcacaa gatatttttt
     5401 cgtttcattc atattcagtt ggatcctttt catatgacta ttctaccaaa tgggacattt
     5461 tcacgttcga tttactaaag acattatcac attcgaatta cacaagatgc agatagtaaa
     5521 aaaaaatgta gacattaccg taaaaaagtg atataatcgt atgatgttca aggtataggt
     5581 gttaatcatg agtagacgtt ttaaaaaatc acgttcacag aaagtgaagc gaagtgttaa
     5641 tatcgttttg ctgactattt atttattgtt agtttgtttt ttattgttct taatctttaa
     5701 gtacaatatc cttgctttta gatatcttaa cctagtggta actgcgttag tcctactagt
     5761 tgccttggta gggctactct tgattatcta taaaaaagct gaaaagttta ctatttttct
     5821 gttggtgttc tctatccttg tcagctctgt gtcgctcttt gcagtacagc agtttgtggg
     5881 actgaccaat cgtttaaatg cgacttctaa ttactcagaa tattcgatca gtgttgctgt
     5941 tttagcagat agtgagattg agaatgttac gcaactgatg agtgtgacag caccgactgg
     6001 gactgataat gaaaacattc aaaaactact atctgatatc aaatcaagtc agaataccga
     6061 tttgacggtc aatcagagtt cgtcttactt ggcagcttat aagagtttga ttgcaggaga
     6121 gactaaggcc attgtcttaa atagtgtctt tgaaaatatc atcgaatcgg agcatccaga
     6181 ttacgcatcg aagataaaaa agatttatac caagggattc actaaaaaag tagaagctcc
     6241 taagacgtct aaggatcagt ctttcaatat ctatgttagt ggaattgata cttatggtcc
     6301 gattagttcg gtgtcgcgtt cagatgtcaa tatcctgatg actgtcaatc gagataccaa
     6361 gaaaattctc ttgaccacaa cgccacgtga tgcttatgta ccaatcgcag atggtgggaa
     6421 taatcaaaaa gataaattga cccatgcggg catctatgga gttgattcgt ccatccacac
     6481 tttagaaaat ctctatggag tggatatcaa ttactatgtg cgattgaact tcacttcttt
     6541 cttgaaattg atcgatttgt tgggtggggt agatgtttat aatgaccaag attttacatc
     6601 tctacacggg aagttccatt tcccagttgg aaatgtccac ttagattctg agcaggctct
     6661 gggatttgtt cgtgagcgct attccctagc ggatggtgat catgaccgcg ggcgcaatca
     6721 acaaaaggtg attgcggcca tccttcaaaa attaacttcg agcgaagcgc tgaaaaatta
     6781 tagtaggatc attgatagct tgcaagattc tatccaaaca aacatgccac ttgagaccat
     6841 gataaacttg gtcaatgctc agttagaaag tggtggaacg tacaaagtga attcgcaaga
     6901 cttgaagggt atgggacgga cggatcttcc ttcctatgcg atgccagata gtaacctcta
     6961 tatgatggaa attaacgaca gtagccttgc atctgtcaaa actgctattc aggatgtgtt
     7021 ggagggcaga tgaaatgatt gatattcatt cgcacatcgt ctttgatgta gatgatggtc
     7081 ccaagtcaag agaggaaagc aaggctctct tggcagaatc ctacaggcag ggggtgcgaa
     7141 ccattgtctc tacctctcac cgtcgcaagg gcatgtttga aactccggaa gagaagatag
     7201 cagaaaactt tcttcaggtt cgggaaatag ctaaggaagt ggcgagtgac ttggtcattg
     7261 cttacggggc tgaaatttac tacacatcag atgttttgga taagctggaa aaaaatcgga
     7321 ttccgaccct caataatagt cgttatgcct tgatagagtt tagtatgaac accccttatc
     7381 gcgatattca tagtgccttg agtaaaatat tgatgttggg aattactcca gtcattgccc
     7441 acattgagcg ctatgatgca cttgaaaata atgaaaaacg cgttcgagag ctgatcaata
     7501 tgggctgtta cacgcaagta aatagttcac atgtcctcaa atccaaactt tttggagaac
     7561 gttataaatt catgaaaaaa agagctcagt attttttgga gcgtgacttg gttcatatca
     7621 ttgcaagtga tatgcataat gtggacagca gacctcccca tatggcagaa gcatatgacc
     7681 ttgtttccca aaaatacgga gaagcgaagg ctcaggaact ttttatagat aatcctcgaa
     7741 aaattgtaat ggatcaacta atttaggaga aatgatgaaa gaacaaaaca cgatagaaat
     7801 cgatgtattt caattactta aaaccttgtg gaaacacaag ctaataattt tattagtggc
     7861 acttgtgaca ggggcgggag cttttgcata tagcactttt attgttaagc cagaatatac
     7921 aagcaccacg cgtatttacg tagtcaaccg taatcaagga gataagccgg gactgacgaa
     7981 tcaggacttg caggcaggaa cttatctggt aaaagactac cgcgaaatta tcctttcgca
     8041 agatgtattg gaaaaggtag cgacaaattt gaaattggat attccagcaa aaacgttaac
     8101 tagcaaagtg caagtgactg taccagccga cactcgtatc gtctcaatct ctgtcaagga
     8161 taaacaacca gaggaagcca gtcgcattgc taattctcta cgagaagttg ctgcagaaaa
     8221 gatcatcgct gtaacgcgag tatctgatgt aacgacgctt gaagaagcgc gaccggctat
     8281 gactccctct tctccaaatg ttcgacgcaa caccttagtt ggttttcttg gaggagccgc
     8341 cgtaacagta attactgttc ttttgattga gttgctcgat acccgtgtaa aacgtcctga
     8401 agatgttgaa gatgtactgc aaatgccact tctaggagtc gttccagatt ttgataaaat
     8461 gaaataggag gaagttatgc caacgttaga aatctcacag gcgaaattag aacttgtgaa
     8521 aaaggcagag gaatattata atgctttgtg cacgaaccta cagttaagtg gagatgattt
     8581 gaaagtattt tctatcactt ctgtgaaagc aggagaagga aaaacaacga cttccaccaa
     8641 tatcgcttgg gcttttgcgc gtgcaggtta caaaacgctt ctgattgatg cagacatgcg
     8701 taactccgtt atgtcaggtg tctttaaatc aagggaaagg attactggac taacagaatt
     8761 tctatcaggg acaacagacc tgtcacaggg gctttgcgat accaatattg agaatctctt
     8821 tgtgattcag gctggctctg tgtcaccaaa tccgacagcc cttcttcaaa gtaagaattt
     8881 cagtacaatg cttgaaacct tgcgtaaata ttttgactat atcgtcgtag acactgcccc
     8941 tgtcggtgtc gtgattgatg cggctatcat tatgcagaaa tgtgatgctt ctattttagt
     9001 gacgaaggca ggtgaaacaa aacgacggga tcttcaaaaa gcgaaagaac agttggaaca
     9061 aactgggaag tcgtgtttag gagttgtgtt gaataaactc gatacttcag tagagaaata
     9121 cggttcttat gggagttatg ggaattacag gaaacagaaa aaataggttg agggatagaa
     9181 atgagtgaaa aactagcaaa gccttcagta gccatagtcc agagttttct tgttatttta
     9241 ttgacttatc tacttagcgc tgtgagagaa acagagattg tttcaacaac agctattgta
     9301 ctttatatcc tccactattt tgtcttttat atcagtgatt atggacggaa tttctttaaa
     9361 aggggatatt tgattgaact tgtccagaca ttgaaatata tcctattctt tgcgctagcg
     9421 attagtattt ctaatttttt cttagaagat cgatttagta tttccagaag aggcatgatt
     9481 tacttcctct tattacatgt tttcttagtc tatgtgctaa acccatttat caagtggtat
     9541 tggaagcggg cttatcccaa ctttaaagga agtaagaaga ttctcctact tacagcaact
     9601 tctcgtgttg aaaaggtact ggatagacta atagaatcag atgatgttgt tggggagttg
     9661 gtagccgtca gtgttttaga taaaccagat tttcagcatg atgatttaaa ggttgtagca
     9721 gagggggaga tagtaaattt tgcgactcat gaggtggtcg atgaagtctt tatcaatctt
     9781 ccaagtgaaa aatacaatat tggagagctt gtctctcagt ttgaaacgat gggaattgat
     9841 gtaacagtca atctaaatgc tttcgattgg gcacgtaaca agcaaatttg tgagatggca
     9901 ggactaaatg ttgtgacttt ttctacaaca ttttataaga ctagccatgt gattgctaag
     9961 cgggttattg atattgtcgg ttccctggta ggtttgatat tatgtggtct agtcagtatt
    10021 gtgctggttc ctttgattcg aaaggatggg ggttctgctt tttttgctca gacgcgtata
    10081 ggaaaaaatg gccgccattt tactttttat aagtttcgct ctatgtgtgt ggatgctgag
    10141 gagaaaaaaa gagaactcat ggaacaaaat accatgcagg gtggaatgtt taaggtggac
    10201 gatgatcctc gtatcacgaa aattggtcat tttatacgga agacgagctt ggacgagcta
    10261 ccacagtttt ataatgttct aaagggagat atgagtttgg taggtacacg accaccaaca
    10321 gtggacgagt atgagcacta taccccagaa caaaaacgtc gcctaagttt taaacctggc
    10381 ataacaggtt tatggcaggt cagcggacga agtgaaatca aaaatttcga tgaagttgtc
    10441 aaattagatg tggcctatat tgatggttgg acaatctgga aagatattga aattttattg
    10501 aagacagtta aggttgtatt gatgaaggat ggagcgaaat agttcatatg aatgagagaa
    10561 ttcaaatatt aggaattact atagatccat taacgatgaa agaaacagtg gatgctgttg
    10621 aacagtatgt tttaaagaag caccctttgc acttgatggg agttaacgcg gataaaatta
    10681 atcagtgcta tgaagatgag aaaatcaaaa aaatcgttaa tgaatcagga ataattaatg
    10741 ccgacggtgc ttcagtagtt ttagcgagta agtttttagg aacaccagtt cctgagcgag
    10801 ttgctggaat agacttgatg aaacatttac tagagttgtc taatgaaaaa ggatactcgg
    10861 tctacttttt tggagcaaaa gaagacgttt tacaagatat gttaaaaatt tttgaagaaa
    10921 gatatccagc tctaaatgtt gttggctata gaaatggata tttttcgcct gaagatgaaa
    10981 aacatattca ggaagatatt aaagagaaaa aaccagattt tgtgtttgta ggaattactt
    11041 ctcctaagaa agaatatatt atccaatcat tcatggataa tggaattaat gctgtgttta
    11101 tgggggttgg tggaagtttt gatgtactat cagggcatat caaacgagct ccattatgga
    11161 tgcaaaatgc tcacctagag tggttgtttc gtgtggcaaa tgagcctaaa cgtctcttta
    11221 agcgttattt tgtagggaat gtttcattta taagaaaagt tttaagagcg aaaaggggat
    11281 ataaactttg aaccaaacag aaatagttaa attgattcag aaagttgaat tggatgctat
    11341 aaaagagttt caaaaaattt gtaaagagaa caatatagat ttcttccttc gtggggggag
    11401 tgtacttggg gcagtcaaat atgatggctt tattccatgg gatgatgaca tggatattgc
    11461 tgtgcctcgt gaaggctacg ataaacttcc aggtatcttt aaggatagaa ttatcgcagg
    11521 caaatatcag gttcttgctt atcaatattg cgatacttta cattgctact ttcctcgctt
    11581 attcctttta gaagatgaaa gaaaacgttt gggattgcca cgaaatacca atttaggttt
    11641 gcatttgatt gatatcattc ctttggatgg tgccccaaat cattcatttt taagaaagct
    11701 ttacttcggt aaagtatact ggtatcgttt tttagcaagt ttaggaacta cctatgttgg
    11761 agaccatgtg gatatgcatt ccgctaagca aaaactaatc attggtttct ttaaaaagct
    11821 aggatttgca aaactattcc ctcaaaatta tgtgtacaga cgcttggata atctctataa
    11881 aaagtatggt tggaaaaagc agaagtatgc tgggacaatt aatgcatctc tgtttgccaa
    11941 agaagtcatg ccagtagaga tttggggaga aggagtagag aagccttttg aggatgcctt
    12001 ctttaaagtt cccacggagt atgaccgcta tctgaaaaga ctttacggag aaaactatct
    12061 tcacgaagag cctagtgatg atgaaaagaa atcgcattta ggaggacaat aatttgtttt
    12121 gttatattat tttgcattac aaagtcttag aggaaactat ttcttgtgtt aaatcaataa
    12181 aagaaggcaa ttccaataca aagcagatcg ttattattga taatttttct aataacggta
    12241 cgggtgaaaa actacaagag ctgtatgagt cagattcaga gattgatgtc ttgattaacc
    12301 atgaaaatgc tggtttcgct cgaggtaata atgtagcata tcagtttgct aaggaaaagt
    12361 acaaacctga ttttatggtt atcatgaata atgatattga gatagaaaca gaagagtttg
    12421 aaaaaatcgt gacagatatc tatcggaagg aaaaattcca tttgttagga ccagatatct
    12481 tttcgacgac gtatcagctt caccaaaacc caaaacggtt gacgcattat acttatgaag
    12541 aggttaaggc tctcaatgaa aaatttaaga aagggagcca agttagtcta gcattaaaaa
    12601 ttaaatgttg gttgaagtct agtaaagttc ttcggacagc aatctatcaa aataggcgta
    12661 aaaagaaatc agtagactat agaaaacagg tagaaaaccc aattcttcat ggttcgttta
    12721 ttgtatattc tagagatttt attgagaaag aggagtatgc ttttaatccc aataccttct
    12781 tctattatga aacagagata ttagattatg aagctgagtt aaaaggatat aagagaattt
    12841 atacaccgaa gattaaggtc ttgcaccatc aaaatgttgc aaccaatcaa gtctatacaa
    12901 acttagtaga aaaaaccttg ttttcaaaca aatgcaactt tgaatccact agttattttt
    12961 tgaagttgat gaaagaaaac gagggtgtgt aaatgactta tttattttta ctctgcctga
    13021 ccttattctt attaactttc ttctattttt ttgctttcaa tcaagatttg atagctccgc
    13081 cagttgtcat gtctgttatg tttctcatta gttctgtatt tgcccttatt aatgtgcaaa
    13141 actggaatat tgagtatagt ggtttagctt atcttttgat tattagtggc attattgttt
    13201 tttcaatgcc tctcttggct cttaattcgc ctattctaaa taccaagatt aaagtgacgg
    13261 atcggctcat tgatatccaa ttctggaaaa tagctcttac tattatagtt gacctcatta
    13321 ttctatatct ttataggaga gagattcata atcttgcact tagccatggt tatacgggtt
    13381 caaattttca gtggttcttt agaaatgcta ccagttatga aggtgagcta acagtgcgaa
    13441 cttcgattcg ggtcctcatt cgtatcattg acgtatctgc ttatattttt ggatatactt
    13501 ttattaataa tttcttcatt tatagtcata aacgctctaa agatttactg ctcttagttc
    13561 cattcttgat ttttatttct aaaaccttat tatctggggg tagattggat attataaaaa
    13621 ttttaattgc gtatgttgta atggcctata ttcagcaaaa acgaaaagtt ggctgggata
    13681 aggtcatctc ccataaatat atgagacttg gttttgtagg cttgatagct gggattccta
    13741 ccttttacta ttctttattc ttatctggac gttctacgac tagaactgta tttgaaagta
    13801 tttcaaccta tttaggaggt tcgattcagc attttaatca gtatattcaa aatcctattg
    13861 gagtagctga ggtttttggc gatgagtcat ttgtagctat tatgaatatt ttgggtaatc
    13921 ttggctttgt caattacaat agtaccgttc acttagaatt tcggcagtta gggattacta
    13981 tgggtaacgt ttatactttc tttagaagac cctggcatga ctttgggtta gtcggtatgt
    14041 atatcttttc ctttgttgtc ggtgtgtttt ttgctatttt ttatttgaaa ttaagaaaga
    14101 gccgtgctgg ttttaaattg gatattcata caattattta ctcttatttc ttttattgga
    14161 tttttttatc gtccattgag caatactcgt ttacaacaat cagtttgttt acacttgtat
    14221 ttatagtctt ggtttaccta atggctttct tctattggaa tttggatttc cacagaggaa
    14281 aactagtaat taaactctct gacacaagta ttaaatcgga aaactgaaaa caataaaaat
    14341 agagaggata gttgatgaat agcaaaatta aaaatatact aactaatttc tcttatgtca
    14401 tttcttcaaa tcttttgaca gtcttgacct cttccttggt tgtcttaatt ttccccaaat
    14461 taatgggggt aacagagtac agttactggc aactttatat tttttatctg acctatatcg
    14521 gttttttcca cttgggttgg attgatggga tttatcttaa atatggtggc ttagaatata
    14581 ataatttaga tagaaaacag ttttattctc agatgattct attttctagc ttcttaatga
    14641 ttatctcctt gctattgttt ggtttaaatg tattagctgt aacagattcg aatgcacgat
    14701 atatttataa tatgactatt ataagtatga taatcacaaa tttaagaacg ctctacgtgt
    14761 atgttttgca gatgacaaac cgcttgaagg atagttccat cattctactc agtgatcgcg
    14821 ttttatatgt gctcctttta ttcctgttta ttgtatttgg ttggcatgaa tacaaggtta
    14881 tgattttggc agatattcta ggtcgatcat tttctctcat actttccttc tggatttgta
    14941 aagatattgt gtttcagcct ttgtcaaaat tcatctttaa tataaaagag tcttttgata
    15001 atatccgagt tggtatcaat ttaatgttat ccaacatcgc aagtagtttg attatcggta
    15061 ttgttcgtct aggaatccaa tggaactgga atatcgaaac attcgggaaa gtatctctga
    15121 ccttgagtgt ttccaatcta ttgatgactt tcattaatgc cattggacta gtcgtctttc
    15181 cgctattaag acgaacaaaa gcagaaaact tacctaagat ttattctaat ttaagaaacg
    15241 ttttgatgct tatcatgttc gcgattttgc tcatttacta tcctttaaaa attgtattag
    15301 acctctggtt gccagcctat caagatgcct tgattttcat gacccttatt ttccctatgt
    15361 cagtctatga agggaaaatg gcattggtca ttaatactta cttaaaggca ttaagaatgg
    15421 aaagagatat ccttagagtc aatgccttag taatgttgat cagtatggga gtgaccctgg
    15481 ttacaacata cctgttaaat agtttggagc tgactgttgt atcgatagtt gttttgctag
    15541 ctttgagaag tatcatagcc gaattaattc tgtctaaaaa actggatgtt tcggttaaga
    15601 aggatattgt attagaattt cttttgacgc ttgtctttat ttcttcaagt tggtatttgc
    15661 cgattgggct ggcagtaatt gtctacacaa tagcctacgg tttatatctc tatttgaaac
    15721 acgaagatat caaaacctat ttagcttact ttaaagctag taaaaaaaca tcaaattaaa
    15781 aaattatata atgattaagt ggtagattct atttctaccg ttttagatat tcgggaggta
    15841 atgatgaaaa aaattatgtt agtattcggt acacgtccgg aagccatcaa aatgtgtcca
    15901 ttagtcaatg agttgaaaaa acacgaagat atggaaacaa ttgtgtgtgt tactggacaa
    15961 cacaaagaga tggttagtcc tgttttagat ttatttggtg ttgtaccaga ttatgattta
    16021 gaaattatga aggctaacca aaccttgttc tctatcacaa ctagtatctt ggaaaagata
    16081 aaaccagttt tagagaagga acaaccagat attgtcctag ttcacggtga cactacgaca
    16141 acttatgcag cagccttggc agcattctat ttgggaatta aagtaggaca tgttgaagct
    16201 ggtttgcgaa cgtacaattt acaaagtcca tttcctgaag aatttaacag gcaatcgaca
    16261 tcaatcattg caacttacca ttttgctcca actgagttgg ctaaagaaaa tctcttaaaa
    16321 gaaggtagag agaatgttta tgtgactgga aatactgtca ttgatgctct tacaactact
    16381 gttcaagagg attatacaca cactcattta gatttaaacg ctaacaatcg tctcatctta
    16441 ttgactgctc atagacgcga aaatctcggc gaaccgatga gacatatgtt tagagcagtt
    16501 aaacgagtat tgaatgaata cgaagatgtt aaagtcattt atccaattca taagaatcct
    16561 ttggtacgtg aaacagctgc agagatcttt ggagatacag aacggattca aattattgaa
    16621 cctttggatg ttcttgattt ccacaacttc atgaaccaaa gttatatgat tttaacagat
    16681 tctggcggag ttcaggaaga agcaccttct ttaggaaaac ctgtattggt tatgcgcgat
    16741 actactgaaa gacctgaagg agtagcggca ggaaccttga aattagtagg aactgacgaa
    16801 gaaacaattt atcaaaactt caaactactt ctagatgatt ctggagaata caaaaaaatg
    16861 agtcaggcta gcaatccata cggtaatggt gatgctagtc aacagattgt tcagattttg
    16921 cgtgggattt aagaagattc tctaaaagag tctaagagga atccactcca caaacataaa
    16981 actcttatgc tatcatttat gattgagata gactcataac ttaatatttt tatcctaaga
    17041 aaattcgttc atattcaata tgaatgaaaa acaacgaaga aaggttattt cattatgaaa
    17101 ggtattattc ttgcaggcgg ctcaggtacc cgcctgtacc cacttactcg ggctgcgtca
    17161 aaacagctga tgccggttta tgataaacct atgatttatt atccgttgtc gacattaatg
    17221 ttggctggaa ttaaagatat tttgattatc tcaactcctc aagatttgcc ccgttttaag
    17281 gacttgctct tggatggttc cgaatttggg atcaagcttt cctatgcgga acaacctagt
    17341 cccgatggac ttgctcaggc ttttcttatc ggtgaagaat ttatcggtga cgatagcgtt
    17401 gccttgattt taggcgacaa tatctaccat ggtcctggtc tgagcacaat gcttcaaaaa
    17461 gcagccaaga aagagaaagg tgcgactgtt tttggctacc aagtgaagga tccagagcgt
    17521 tttggtgtgg ttgagtttga tacagacatg aatgctattt ctatagaaga aaaaccggag
    17581 tatcctcgct ccaactatgc agtgacagga ctgtatttct atgataatga tgttgtggag
    17641 attgctaaac agatcaaacc tagtgctcgt ggcgagttag aaattacaga cgttaacaag
    17701 gcttacctaa atcgtggtga cctttctgtt gagctgatgg ggcgtggttt tgcctggttg
    17761 gatacgggaa cccatgaaag cttgctagaa gcttctcagt atattgaaac ggttcagcgt
    17821 atgcagaacg ttcaagtggc aaatctagaa gaaatttcct atcgtatggg ctacattagt
    17881 cgtgaagacg tgctggaatt ggctcagcct cttaagaaaa atgaatacgg acaatatttg
    17941 ctccgtttga ttggagaagc atagatgtca gataattttt ttggaaagac acttgtggta
    18001 cgcaagattg atgctatacc aggactgcta gagtttgaca ttcccgttca tggagacaat
    18061 cgtggttggt ttaaggaaaa tttccagaag gaaaagatgc taccgcttgg ttttcctgaa
    18121 agcttctttg ctgcagggaa actgcaaaat aacgtcagct tttctcgcaa aaatgttctt
    18181 cgaggactcc atgctgaacc ttgggacaag tatatctctg ttgcagacga tgggaaggtt
    18241 ttaggatctt gggtagattt gcgtgaaggc gagacctttg gaaatgttta ccagacagag
    18301 attgatgcta gcaagggaat ttttgtccct cgtggcgtag ccaacggttt tcaagtcctc
    18361 tcagatacag tttcctatag ctatctggtc aatgactact gggctcttga actcaaaccc
    18421 aaatatgcct ttgttaacta tgctgaccca gcattgggaa ttgagtggga aaacctagcg
    18481 gaagctgagg tttcagaggc agacaaacac catcctctat taagggatgt caaaccactt
    18541 acgaaagacg agttgtaaaa ggaaacatta tgactgaata caaaaaaatt atcgtgacag
    18601 gtggagctgg ttttatcggt tctaactttg tccactatgt ttacaataac tttccagatg
    18661 tccatgtgac agtgctggac aagctgactt atgcaggtaa tcgtgccaat atcgaggaaa
    18721 ttttaggcga ccgtgttgag ttggttgttg gagatattgc tgatgcagcc ttggtagaca
    18781 agttggcggc tgaagcggat gctatcgttc actatgcggc agaaagccac aatgataact
    18841 cgctcaatga cccgagtcca tttatccaca ccaactttat cggaacttac acacttttag
    18901 aagcggctcg taaatacgac attcgtttcc accatgtatc gactgatgaa gtctatggtg
    18961 acctgcctct gcgtgaagat ttgccaggtc atggggaagg cctaggtgag aaatttaccg
    19021 ctgaaaccaa gtacaatcca agctcgcctt actcatcaac caaggctgct tcagacttga
    19081 tcgttaaagc ttgggtgcgc tcatttggtg ttaaagcaac gatttctaac tgttcaaaca
    19141 actatggtcc ataccagcat atcgagaagt tcattccgcg ccagattacg aatatcttga
    19201 gcggtatcaa gccaaaactc tacggagaag gtaagaatgt gcgtgactgg attcacacca
    19261 atgaccattc atcaggcgtt tggacgattc tgaccaaggg tcaaatcggt gaaacttact
    19321 tgatcggtgc tgacggtgag aagaacaaca aggaagtgtt agagctcatt ctcaaggaga
    19381 tggggcaacc cgctgacgct tatgaccatg tgaccgatcg agctggtcac gacttgcgtt
    19441 atgcgattga tgctagcaag ctccgtgatg agttggggtg gaagccagag tttaccaact
    19501 ttgaagcagg cctcaaagag accatcaagt ggtacacaga taaccaagac tggtggaaat
    19561 ctgaaaaaga agcagtagag gctaactatg ctaaaacgca acaagtgatt aaataataag
    19621 atactaaaaa agcaagagac ctcgaggtct cttgcttttc tagtatgtag taagctttta
    19681 ttttcttact tcttgtttat aaaactcttt aagggcatct tgccaggttg ggatgacgaa
    19741 acctgttgcc tttgcctttg ccaaactcat agttgagttg agaggtcgtt tagccttagc
    19801 ggggaacttg ctagagtcta ctggcactac ttcaacatca ctgtccttaa gaatctcgct
    19861 agcaaagtca taccaagtgg tgtcttcagc tgcgtcattt gataagtgat agtagccata
    19921 ctctttttga ttttcagtca cataggtcat gaattcggcc aaggtccgtg tccaagttgg
    19981 gcgaccgtat tggtcgttga ccactgtgag cgttttatgg gttttggcta ggctttgcat
    20041 ggtaaagaca aagttttttc cataatttcc aaaaacccaa gcagtacgaa tgatgtaatg
    20101 ctgtgacgta aggttttcaa caagttcttc tcccattcgc ttggtacgtc cgtactctgt
    20161 ttgcggatca ggtatgtcat cgacttccca ctcttgtcct actggtttct ttccatcaaa
    20221 gacgtagtct gttgagatat agaccagaat agctccgtat ttctcagagg ccttggctac
    20281 attttcagtt ccagttacgt tgatggcaaa atccagttct ttcccttcat cttcggctgc
    20341 atcaacagca gtgtaggctg cacaatgata gactagagtt ggcttaacct cagcaaagac
    20401 tttttcaacc atttcagaat tagtgatatc catttcggtc acatccactg caacatagtc
    20461 cacatttcgc tcatctagta aataacgtag ttcggtacca agttgaccat ttgcacctgt
    20521 aattaagatc atatttttct ccttgatagt tcttatactt aattatatca aaaatataga
    20581 aaaatcggtt tttagtgaac ccttttttgg tataaacttt gttagtgaag cgcaagagag
    20641 ccgaatagtt attactttag aatatcctta aaagtatttt tcagaattta ccaaattaat
    20701 taaaaaattc agaaaattat attgacatct ctctgaaaag agtctataat agagagaaag
    20761 ttttaaagga gaagatgatg aaaagttcaa aactatttgc ccttgcgggt gtaaccttat
    20821 tggcggcgac tactttagct gcatgctctg gatcaggttc aagcactaaa gctgagaaga
    20881 cattctcata catttatgag acagaccctg ataacctcaa ctatttgaca actgctaagg
    20941 ctgcgacagc aaatattacc agtaacgtgg ttgatggttt gctagaaaat gatcgctacg
    21001 ggaactttgt gccgtctatg gctgaggatt ggtctgtatc caaggatgga ttgacttaca
    21061 cttatactat ccgtaaggat gcaaaatggt atacttctga aggtgaagaa tacgcggcag
    21121 tcaaagctca agactttgta acaggattaa aatatgctgc tgataaaaaa tcagatgctc
    21181 tttaccttgt tcaagaatca atcaaagggt tggatgccta tgtaaaaggg gaaatcaaag
    21241 atttctcaca agtaggaatt aaggctctgg atgaacagac agttcagtac actttgaaca
    21301 aaccagaaag cttctggaat tctaagacaa ccatgggtgt gcttgcgcca gttaatgaag
    21361 agtttttgaa ttcaaaagga gatgattttg ccaaagctac ggatccaagt agtctcttgt
    21421 ataacggtcc ttatttgttg aaatccattg tgaccaaatc ctctgttgaa tttgcgaaaa
    21481 atccgaacta ctgggataag gacaatgtgc atgttgacaa agttaaattg tcattctggg
    21541 atggtcaaga taccagcaaa cctgcagaaa actttaaaga tggtagcctt acagcagctc
    21601 gtctctatcc aacaagtgca agtttcgcag aacttgagaa gagtatgaag gacaatattg
    21661 tctatactca acaagactct attacgtatc tagttggtac aaatattgac cgtcagtcct
    21721 ataaatacac atctaagacc agcgacgaac aaaaggcatc gactaaaaag gctctcttaa
    21781 acaaggattt ccgtcaggct attgcctttg gatttgaccg tacagcctat gcctctcagt
    21841 tgaatggaca aactggagca agtaaaatct tgcgtaatct ctttgtgcca ccaacatttg
    21901 ttcaagcaga tggtaaaaac tttggcgata tggtcaaaga gaaattggtc acttatgggg
    21961 atgaatggaa ggatgttaat cttgcagatt ctcaggatgg tctttacaat ccagaaaaag
    22021 ccaaggctga atttgctaaa gctaaatcag ccttacaagc agaaggtgtg actttcccaa
    22081 ttcatttgga tatgccagtt gaccagacag caactacaaa agttcagcgc gtccaatcta
    22141 tgaaacaatc cttggaagca actttaggag ctgataatgt cattattgat atccaacaac
    22201 tacaaaaaga cgaagtaaac aatattacat attttgctga aaatgctgct ggcgaagact
    22261 gggatttatc agataatgtc ggttggggtc cagactttgc cgatccatca acctaccttg
    22321 atatcatcaa accatctgta ggagaaagta ctaaaacata tttagggttt gactcagggg
    22381 aagataatgt agctgctaaa aaagtaggtc tatatgacta cgaaaaattg gttactgagg
    22441 ctggtgatga ggctacagat gttgctaaac gctatgataa atacgctgca gcccaagctt
    22501 ggttgacaga tagtgctttg attattccaa ctacatctcg tacagggcgt ccaatcttgt
    22561 ctaagatggt accatttaca ataccatttg cattgtcagg aaataaaggt acaagtgaac
    22621 cagtcttata taaatacttg gaacttcaag acaaggcagt cactgtagat gaataccaaa
    22681 aagctcagga aaaatggatg aaagaaaaag aagagtctaa taaaaaggct caagaagatc
    22741 tcgcaaaaca tgtgaaataa ctgttgcaaa atataagaaa ggatttagta tttctcttga
    22801 atgctgaatc cttttttaca tttgtaaaga aagattctaa atgtactgac ccccaaaagt
    22861 tggagcctct ttttgtcaga atagagaaaa tttttgttaa ttttacttgt ttcctattgc
    22921 tttctcagtt attatttgtt atattaaaag tataattatt tttatttatc agggttaagc
    22981 attgcacttt cagaggaagg agtatttttt aaaaagaaaa tgtaaacgct tgctcaaaaa
    23041 tgaaaggatt tagaagttta tgaataaagg attatttgaa aaacgttgta aatatagtat
    23101 tcggaaattt tcattaggtg ttgcttctgt tatgattgga gctgcattct ttgggacaag
    23161 tcaggttctt gcagatagcg tgcagtctgg ttccacggcg aacttaccag ctgatttagc
    23221 tactgctctt gcaacagcaa aagagaatga tgggcgtgat tttgaagcgc ctaaggtggg
    23281 agaagaccaa ggttctccag aagttacaga tggatctaag acagaagaag aactattagc
    23341 acttgaaaaa gaaaaaccgg ctgaagaaaa accaaaagag gataaacctg cagttgctaa
    23401 acctgaaacg cctaagacgg taacccctga atggcaaacg gtagagaaaa aagaacaaca
    23461 gggaacagtc actatccgag aagaaaaagg tgtccgctac aaccaactat cctcaactgc
    23521 tcaaaatgat aacgcaggta aaccagccct gtttgaaaag aagggcttga ccgttgatgc
    23581 caatggaaat gcaactgttg atttaacctt caaagatgat tctgaaaagg gcaaatcacg
    23641 ctttggtgtc ttcttgaaat ttaaagatac caataataat gtttttgtcg gttatgacaa
    23701 ggatggctgg ttctgggagt ataaatctcc aacaactagc acttggtata gaggtagtcg
    23761 tgttgctgct cctgaaacag gatcaacaaa ccgtctctct atcactctca agtcagacgg
    23821 tcagctaaat gccagcaata acgatgtcaa tctctttgac acagtgactc taccagctgc
    23881 ggtcaatgac catcttaaaa atgagaagaa gatccttctc aaggcgggct cttatggcaa
    23941 tgaccgtacg gttgtcagcg ttaaaacaga caatcaagaa ggcgtaaaag cggatgatac
    24001 tcctgcccag aaagaaacag gtccggttgt tgacgatagc aaggtgactt atgatacgat
    24061 ccagtctaag gtacttaagg cagtaattga ccaagccttc cctcgtgtca aggaatacac
    24121 cttgaatgga catactttgc caggacaggt gcaacagttc aaccaagtct ttatcaataa
    24181 ccaccgaatc acccctgaag tcacttataa gaaaatcaat gagacaacag cagagtactt
    24241 gatgaagctt cgcgatgatg ctcacttaat caatgcggaa atgacagtac gcttgcaagt
    24301 tgtggacaat caattgcact ttgatgtgac caagattgtc aaccacaatc aagtcactcc
    24361 aggtcaaaag attgatgacg aaagaaaact actttcttct attagtttcc tcggcaatgc
    24421 tttagtctct gtttctagtg atcaaactgg tgctaagttt gatggggcaa ccatgtcaaa
    24481 caatacgcat gtcagcggag atgatcatat cgatgtaacc aatccaatga aggatttggc
    24541 taagggttac atgtatggat ttgtttctac agataagctt gctgctggtg tttggagtaa
    24601 ctctcaaaac agctatggtg gtggttcgaa tgactggact cgtttgacag cttataaaga
    24661 aacagtcgga aatgccaact atgtaggaat ccacagctct gaatggcaat gggaaaaagc
    24721 ttataagggc attgttttcc cagaatacac gaaggaactt ccaagtgcta aggttgttat
    24781 cactgaagat gccaatgcag acaagaacgt tgattggcaa gatggtgcca ttgcttatcg
    24841 tagcattatg aacaatcctc aaggttggga aaaagttaag gatatcacag cttaccgtat
    24901 cgcgatgaac tttggttctc aagcacaaaa tccattcctt atgaccttgg atggtatcaa
    24961 gaaaatcaat ctccatacag atggtcttgg gcaaggtgtt ctccttaaag gatatggtag
    25021 cgaaggccat gactctggtc acttgaacta tgctgatatt ggtaagcgta tcggtggtgt
    25081 cgaagacttc aagaccctaa ttgagaaggc taagaaatat ggagctcatc taggtatcca
    25141 cgttaacgct tcagaaactt atcctgagtc taaatacttc aatgaaaaaa ttctccgtaa
    25201 gaatccagat ggaagctata gctatggttg gaactggcta gatcaaggta tcaacattga
    25261 tgctgcctat gacctagctc atggtcgttt ggcacgttgg gaagatttga agaaaaaact
    25321 tggtgacggt ctcgacttta tctatgtgga cgtttggggt aatggtcaat caggtgataa
    25381 cggtgcctgg gctacccacg ttcttgctaa agaaattaac aaacaaggct ggcgctttgc
    25441 gatcgagtgg ggccatggtg gtgagtacga ctctaccttc catcactggg cagctgactt
    25501 gacctacggt ggctacacca ataaaggtat caacagtgcc atcacccgct ttatccgtaa
    25561 ccaccaaaaa gatgcttggg tagggaacta cagaagttat ggtggtgcag ccaactatcc
    25621 actgctaggt ggctacagca tgaaagactt tgaaggctgg caaggaagaa gtgactacaa
    25681 tggctatgtg actaacctct ttgctcatga cgtcatgact aagtacttcc aacacttcac
    25741 tgtaagtaaa tgggaaaatg gtacaccggt gactatgacc gataacggta gcacctataa
    25801 atggactcca gaaatgcgag tggagttggt agatgctgac aataataaag tagttgtaac
    25861 tcgtaagtca aatgatgtca atagcccaca atatcgcgaa cgtacagtaa cgctcaacgg
    25921 acgtgtcatc caagatggtt cagcttactt gactccttgg aactgggatg caaatggtaa
    25981 gaaactttct actgagaagg aaaagatgta ctacttcaat acgcaggccg gtacaacaac
    26041 ttggaccctt ccaagcgatt gggcaaagag caaggtttac ctttacaagc taactgacca
    26101 aggtaagaca gaagagcaag aactaactgt aaaagatggt aaaattaccc tagatcttct
    26161 agcaaatcaa ccatacgttc tctatcgttc gaaacaaacc aatcctgaaa tgtcatggag
    26221 tgaaggcatg cacatctatg accaaggatt taatagcggt accttgaaac attggaccat
    26281 ttcaggcgat gcttctaagg cagaaattgt caagtctcaa ggggcaaacg atatgcttcg
    26341 tattcaagga aacaaagaaa aagttagtct cactcagaaa ttaactggct tgaaaccaaa
    26401 taccaagtat gccgtttatg tcggtgtcga taaccgtagt aatgccaagg cgagcatcac
    26461 tgtaaacact ggtgaaaaag aagtgactac ttataccaat aagtctctcg ccctcaacta
    26521 tgttaaggcc tacgcccaca atacacgtcg taacaatgct acagttgacg atacaagtta
    26581 cttccaaaac atgtacgcct tctttacaac tggatcggac gtatcaaatg ttactctgac
    26641 attgagtcgt gaagctggtg atcaagcaac ttactttgat gaaattcgta cctttgaaaa
    26701 caattcaagc atgtacggag acaagcatga tacaggtaaa ggcaccttca agcaagactt
    26761 tgaaaatgtt gctcagggta tcttcccatt tgtagtgggt ggtgtcgaag gtgtcgaaga
    26821 caaccgcact cacttgtctg aaaaacacga tccatataca caacgtggtt ggaatggtaa
    26881 gaaagtcgat gatgttatcg aaggaaattg gtcactcaag acaaatggac tagtgagtcg
    26941 tcgtaacttg gtttaccaaa ccatcccaca aaacttccgc tttgaagctg gtaagaccta
    27001 ccgtgtaacc tttgaatacg aagcaggatc agacaatacc tatgcttttg tagtcggtaa
    27061 gggagaattc caatcaggtc gtcgtggtac tcaagcaagc aacttggaaa tgcatgaatt
    27121 gccaaatact tggacagatt ctaagaaagc caagaaggta accttccttg tgacaggtgc
    27181 agaaacaggg gatacttggg taggtatcta ctcaactggc aatgcaagta atactcgtag
    27241 tgattctggt ggaaatgcca acttccgtgg ttataacgac ttcatgatgg ataatcttca
    27301 aatcgaagaa attaccctaa caggtaagat gttgacagaa aatgctctga agaactactt
    27361 gccaacggtt gccatgacta actacaccaa agagtctatg gaggctttga aagaggcggt
    27421 ctttaacctc agtcaggccg atgatgatat cagtgtggaa gaagcgcgtg cagagattgc
    27481 caagattgaa gccttgaaga atgctttggt tcagaagaaa acggctttgg tagcagatga
    27541 ctttgcaagt cttacagctc ctgctcaggc tcaagaaggt cttgcaaatg cctttgatgg
    27601 aaacttatct agtttatggc atacatcatg gggcggagga gatgtaggca agcctgcaac
    27661 catggtcttg aaagaaccaa ctgaaatcac tggacttcgt tacgttccac gtggatcagg
    27721 ttcaaatggt aacttgcgtg atgtgaaact tgttgtgaca gatgagtctg gcaaggagca
    27781 tacctttact gcaactgatt ggccagataa caataagcca aaagacattg attttggtaa
    27841 gacaattaag gctaagaaaa ttgtccttac aggtactaag acttacggag atggtggcga
    27901 taaataccaa tctgcagcgg aactcatctt tactcgtcca caggtagcag aaacacctct
    27961 tgacttgtca ggctatgaag cagctttggc taaagctcag aaattaacag acaaagacaa
    28021 tcaagaggaa gtagctagtg ttcaggcaag catgaaatat gcgacggata accatctctt
    28081 gacggaaaga atggtggaat actttgcaga ttatctcaac caattaaaag attctgctac
    28141 gaaaccagat gctccaactg tagagaaacc tgagtttaaa cttagctcta tagtttccga
    28201 tcaaggtaag acgccagatt ataagcaaga aatagctaga ccagaaacac ctgaacaaat
    28261 cttgccagca acaggtgaga gtcaatctga cacatccctc ttcctagcaa gtgttagtct
    28321 agccctatct gctctctttg tagtaaaaac gaagaaagac tagtatttag taaaacctct
    28381 taacaagatt acggaagcag tctctatctt ttccaatgag gtttatagta cagaaaaagc
    28441 ctgagaagat gtcttctcag gcttttgtta agcacataaa tacaatagtg ctatgacaaa
    28501 atcacccaga aaaatctggg tgataaatgt tatggttgtg ctggttgagg attctgattt
    28561 tgttgatcag gggttgtatt tgattgttgc gtattattgt taggattggt agtcgtacta
    28621 ttatttgtgc ttggagtggt tgagctagac tgtgaagttg aactatctga tgatgagctt
    28681 gaactttcag ttgatggggg ttgttgtgga gcaggtgagt tccacgtaga acgagcacca
    28741 tttttaaata cgaattctcc atttctgtag agcccctctg gtatattcca atcttctgga
    28801 ttgcttcctt cagacaggta ggtcatcata gagcggtaaa ctttggcagc gaccgtaagg
    28861 ccattgccta caagtggtgt cagacggtta gaatagcctg tccatacagc cattgaatat
    28921 ttacgcgtat agccagcaaa tagttcatca ggtgctacaa attgagaggt cttgatgtgg
    28981 ttttcaattt cctcgtctgt atagttagag gttcctgttt taccagcctg agggagccaa
    29041 gcaagatagg catttcgtcc agttccataa gtcaagactg ttttcatcat gtcggtcatc
    29101 atataggctg tcgtttcttt catggcacga gttccgacat tagagaactc tttttcactc
    29161 ccatcactaa agacgacttt atggatatac attggtttat agtaagttcc accatttgca
    29221 aaggcagcgt aagcagcagc catcttttca ctacttgctc catatttttt gtctgattcg
    29281 gttgtgttac ttgaaatggc atttgagtag tgaatacttg ggtagtcgat tccgagacca
    29341 tttaggaaag tcttggcgcg gttgagtccg accttgttta gagtttccac ggctgggacg
    29401 tttcgcgatt gttgcagggc gtattgcaag gtgatgttgc caaagtagcc cctatcccag
    29461 ttataaacag gagtatttgt cccagggtaa ttatagggct catcgtgaac gatagtagca
    29521 gttgaatcgt agacaccgta ctccaaggca ggagcatagt ctgtgatcgg tttcatagtt
    29581 gatccccagt cgcggtttgt ttctactgct tggttaattc cgaaggaaac attacttgac
    29641 tgatggcgtg ctcctagctg ggcaatgact ttaccgttag aaacatcaac aatggtagaa
    29701 gcgacttgca attcatcgtc tggataggca acgtattcgt ctgtattgta aatatcccac
    29761 agatgttttt gagcttcttg gtctacattt gtgtagacat ccatcccagt tgtgagcagg
    29821 ttatagcctg tttcttcttc aacttgattg atgacttcct tgaggtaatt atccatgtaa
    29881 gcagggtaat tacttgctga tttgagactt tgtagtccat cagtaattgg tgtattgact
    29941 gctttctcat actgttcagc agagatgtag ccttgatttt tcatttcaga taagaccaag
    30001 tttcggcggt cttgggctgc ttctggatgt gaataggggt catattggtt tggtgcctga
    30061 ggcattccag ccagcaaggc taactgaggt aaacttaaat tattgaggtc tttaccatag
    30121 tagttttgag ctgctgtctg cattccatag ttcccattag acatgtagac cttatttata
    30181 tagtaggtca agatttcttg cttggttgct ttttgttcta actgaatcgc taaccaagct
    30241 tcctgagcct tacgagaaat agtctggtcg gaagtcgaag ttgaaaagta agtcaactta
    30301 atcaactgtt gggtgagagt tgatccacct tggagggaat tgctttgcag attgcgcaag
    30361 aaagctccca ggatacggat ggtatcaatc cccctgtggt cgaagaagcg atggtcttcg
    30421 atagaaacga ttgccttaac caaatctgtg ggaatatcat tagcttgggc attgacgcgg
    30481 cgttcagaac ccaagtcagc aatgagttga tttttattgt cgtagatttt actagaagtt
    30541 gttgcaacta gtttactctc ggataggcta ggagccttgc taacgtagta gaaaaaaact
    30601 cctccgccta agacaatggc tgcgataacc aagcttaaga agctaatgct cagatacttg
    30661 attaggcgca gaatcgttgg tttgttcatc ttgttttacc acctaataaa tgttctttga
    30721 taacattgag ataaggaatt tgagggaagg caccagcctt gatttcatat ccatattctc
    30781 gaatatattc aagtggcatt gatttttgtc ccttatcttg atgatagaag cgaatcaaat
    30841 cgaatgccgg caataagtag gtttcttgct gagaagaaaa gtgaagaagg acaaagcaga
    30901 ttccttgttg ggcaaggact tgttccatat gctgaatctg atgtggatga aaatttttca
    30961 tcggaatcgc acgtttttgt tttgtttcct tgacttcaaa gtcgatgtaa tatccattat
    31021 aaacgccaga atagtccgtc gttgaagctt gtcgaaaata ggcttcaaca atcttggcac
    31081 gacttcgttg tggatagtcc acttgtacga tttgaatagg agttggtttc ttatgtataa
    31141 cagccaagcc ctgagacaaa tagtagtcgt tggtagcatt gatcatcttt tcaaaagaca
    31201 ttcctcgatt tgcgaaattt ttgggttgag aaagagatgt ttgccttttt tgtgatgaaa
    31261 ctttatgtgg atagttgacc ataattctcc ttattggtac aataacatca ctttattata
    31321 ccataatttg cacagaaagg gttaaaaatg gctacagctt tggttttagg ctattctgct
    31381 tttgatttgg gtttattttc tgataaggat cctcgtctta aattgattaa aaaagcgata
    31441 cgtaaagatt tagaggctat ggcagcagat ggggtgtctt ggcttgtgtt tacaggaagt
    31501 ttgggttttg aatattgggt tttagaggtt gctcaggaga tgaaaaccga atacggtttc
    31561 cagttggcca ccatttttgc ttttgaaaac catggggaaa attggaatga aggcaatcag
    31621 atgaaactga gtcgctttaa gcaggtagac tttgtcaaat atgcttatcc tcgttatgaa
    31681 cacaaggggc agttaagaga ttatcagcag tttttgctgg aaaacacgac tagttcctat
    31741 cttttttatg atgaagaaaa tgaaacaaaa ctagcttatt tttaccaaaa gatgaaaaat
    31801 caagaggact attttataaa gagattaaca tttgatgaat taaatgaact tgctgaaaat
    31861 ttttccgaaa attgaagctt tgaccttgat ttttgtttgc ctttttttat ataataatac
    31921 tagcaagcga gaatggagag agacatggca agtattattt tttcagcgaa agatattttt
    31981 gaacaagagt ttggacgtga agtccgtggc tataataaag tagaagttga cgagttttta
    32041 gacgatgtca tcaaggacta tgaaacctat gctgccttgg tcaagtcact tcgtcaggaa
    32101 attgcggatt tgaaggaaga attaactcgt aaaccgaaac cttcaccagt tcaagcagaa
    32161 ccccttgaag cggcaattac aagttctatg acgaattttg atattttgaa acgcctgaat
    32221 agattggaaa aagaagtttt tggtaaacaa attttagata actcagattt ttaagtagtt
    32281 atttgagatg tgcaattttt ggataatcgc gtgaggagaa ttgcttctca tgaggaaagt
    32341 ccatgctagc acaggctgtg atgcctgtag tgtttgtgct aggcgaaacc ataagcctag
    32401 ggacgaggaa tcgttacggc agttgaaatg gctaagtcct tggataggcc agagtaggct
    32461 tgaaagtgcc acagtgacgg agtctttctg gaaacagaga gagtggaacg cggtaaaccc
    32521 ctcaagctag caacccaaat tttggtcggg gcatggagta cgcggaaacg aacgtagtat
    32581 tctgactgct atcagctaga gctgttagtg gtagacagat gattatcgaa ggaagtggtc
    32641 ctagtcactt ctggaacaaa acatggctta tagaaaattg catataggtt ggggctgaga
    32701 aattttctca acctcatttt ttaaagtgga catatagaaa ggtcttgcaa gactgtaaca
    32761 tgaaaaaaga atttaattta attgcaactg tggcagcagg gcttgaggct gtcgttggtc
    32821 gtgaagtgcg agagttgggc tacgattgtc aggttgaaaa tggacgtgtt cgttttcaag
    32881 gagacgtgag agctattatc gaaaccaacc tttggcttcg ggcagcagat cgtatcaaaa
    32941 ttatcgtagg aacgttccca gctaagactt ttgaagagct atttcaggga gttttcgctt
    33001 tagattggga aaattattta ccacttggag ctcggttccc gatttcaaaa gctaaatgtg
    33061 ttaagtccaa acttcacaat gagcctagtg ttcaggctat ttctaagaaa gctgttgtca
    33121 agaaattgca gaaacactat gctcgcccag aaggggttcc tctgatggag aatggcccag
    33181 agtttaagat tgaggtctct attctcaaag atgtggcaac tgtcatgatt gatacgaccg
    33241 ggtctagcct ctttaaacgt ggttatcgta ccgaaaaagg tggcgctcct atcaaggaaa
    33301 atatggcagc agccatttta caactttcta actggtaccc agacaagcct ttgattgatc
    33361 cgacctgtgg ttcggggact ttctgtattg aggcagttat gattgctaga aagatggcgc
    33421 caggtcttcg tcgctctttt gcatttgagg aatggaactg gatcagcgat cgcttgattc
    33481 aagaagtgcg cacagaagcg gctaaaaaag tagaacgtaa gcttgagttg gatatcatgg
    33541 gctgtgatat tgatgctcgc atggtggaaa ttgctaaggc caatgctcag gcagctggtg
    33601 ttgcaggaga tattactttt aagcagatgc gcgtgcagga tttacgttcc gataaaatca
    33661 atggagtaat catttccaat ccgccttatg gtgaacgttt gtcagatgat gcaagggtga
    33721 ccaagctcta tgctgagatg gggcaagtat ttgcaccgct gaaaacttgg agcaaattta
    33781 tcctgactag tgatgaagct tttgaaagca agtatggtag ccaagcagat aagaagcgta
    33841 agttatacaa cggaaccttg aaagtggatc tatatcaata ttttggtcag cgtgtcaaac
    33901 ggcaagaggt aaaatagaaa gggatactca tgagtaaaaa aagacgaaat cgtcataaaa
    33961 aagaaggtca agaaccgcaa tttgattttg atgaagcaaa agagctaaca gttggtcaag
    34021 ctattcgtaa aaatgaagaa gtggaagcag gagtcttgcc tgaggattcc attttggaca
    34081 agtatgttaa gcaacacaga gatgaaattg aggcggataa gtttgcgact cgtcaataca
    34141 aaaaagagga gttcgttgaa actcagagtc tggatgattt aattcaagag atgcgtgagg
    34201 ctgtagagaa gtcagaagct tcttcggagg aagttccatc ttctgaagac atcttactac
    34261 ccttgcctct ggacgatgag gagcaaggct tggatcctct attgctagag gatgaaaatc
    34321 caacagaaat gactgaagaa gtggaagagg agcaaaacct ttctcgtctg gatcaagagg
    34381 actcagaaaa gaaaagtaaa aaaggcttta ttttgaccgt tttggcgctt gtatcagtaa
    34441 ttatttgtgt cagtgcttat tatgtctacc gtcaagtggc tcgttcgacc aaggaaattg
    34501 aaacttctca atcaactaca gccaatcaat cggatgtgga tgattttaat acactttatg
    34561 acgcctttta cacaaatagc aataaaacgg ctttgaaaaa tagccagttt gataaactga
    34621 gtcaactcaa aaccttgctt gataaactgg aaggtagtcg tgaacatacg cttgccaaat
    34681 ctaaatatga tagtctagca acgcaaatca aggctattca agatgtcaat gctcaatttg
    34741 agaaaccagc tattgtggat ggtgtgttgg ataccaatgc caaagccaaa tcggatgcta
    34801 aatttacgga tattaaaact ggaaatacgg agcttgataa agtgctagat aaggctatca
    34861 gtcttggtaa gagccagcaa acaagtactt ctagctcaag ttcaagtcaa actagcagct
    34921 caagctctag tcaagcaagt tcaaatacga ctagtgagcc acaaccaagt agttcaaatg
    34981 agactagaag tagtcgcagt gaagtcaata tgggtctctc gagtgcaggg gttgctgttc
    35041 aaagaagtgc cagtcgtgtt gcctataatc agtctgctat tgatgatagt aataactctg
    35101 cctgggattt tgcggatggt gtcttggaac aaattctagc gacttcacgt tcacgtggct
    35161 atatcactgg agaccaatat atccttgaac gtgtcaatat cgttaacggc aatggttatt
    35221 acaacctcta taagccagat ggaacctatc tctttaccct taactgtaag acaggctact
    35281 ttgtcggaaa tggcgctggt catgcggatg acttggacta ctaagcagtc gttacaaaat
    35341 tctttctttt caaaagtaaa aatgataaaa taaaacaaat gaaacaagag gagtgtcaaa
    35401 tgacaaaagc taactttggt gtcgtaggta tggccgtaat gggtcgtaac cttgccctta
    35461 atattgaatc acgtggttac acagttgcta tctacaaccg tagtaaagaa aaaacggaag
    35521 atgtgattgc ttgccatcct gaaaagaatt ttgtaccaag ctatgacgtt gaaagttttg
    35581 taaactcaat cgaaaaacct cgtcgtatca tgctgatggt tcaagctgga cctggtacag
    35641 atgctactat ccaagccctt cttccacacc ttgacaaggg tgatatcttg attgacggag
    35701 gaaacacttt ctacaaagat accatccgtc gtaatgaaga attggtaaac tcaggtatca
    35761 actttatcgg tactggagtt tctggtggtg aaaaaggtgc ccttgaaggt ccttctatca
    35821 tgcctggtgg acaaaaagag gcctacgaat tggttgcgga tgttcttgaa gaaatctcag
    35881 ctaaagcacc agaagatggc aaaccatgtg tgacttacat cggccctgat ggagctggtc
    35941 actatgtgaa aatggttcac aatggtattg agtacggtga tatgcaattg atcgcagaaa
    36001 gctatgactt gatgcaacac ttgctaggcc tttctgcaga ggatatggct gaaatcttta
    36061 ctgagtggaa caagggtgaa ttagacagct atttgatcga aatcacagct gatatcttga
    36121 gccgtaaaga cgatgaaggc caagatggac caatcgtaga ctacatcctt gatgctgcag
    36181 gtaacaaggg aactggtaaa tggactagcc aatcatctct tgaccttggt gtaccattgt
    36241 cactgattac tgagtcagtg tttgcacgct acatttcaac ttacaaagaa gaacgtgtac
    36301 atgctagcaa ggtgcttcca aaaccagctg ccttcaactt tgaaggagac aaggctgaat
    36361 tgattgaaaa aatccgtcaa gccctttact tctcaaaaat catttcatac gcacaaggat
    36421 ttgctcaatt gcgtgtagcc tctaaagaaa acaactggaa cttgccattt gcagatatcg
    36481 catctatctg gcgtgatggc tgtatcatcc gttctcgttt cttgcaaaag attacagatg
    36541 cttacaaccg cgatgcagat cttgccaacc ttcttttgga cgagtacttc ttggatgtta
    36601 ctgctaagta ccaacaagca gtacgtgata tcgtagctct tgcggttcaa gctggtgtgc
    36661 cagtgccaac tttctcagca gctattactt actttgatag ctaccgttca gctgaccttc
    36721 cagctaactt gatccaagca caacgtgact actttggtgc tcacacttac caacgtaaag
    36781 acaaagaagg aaccttccac tactcttggt atgacgaaaa ataagtaggt cagccatggg
    36841 gaaacggatt ttattacttg agaaagaacg aaatctagct cattttttaa gtttggaact
    36901 ccagaaagag cagtatcggg ttgatctggt agaggagggg caaaaagccc tctccatggc
    36961 tcttcagaca gactatgatt tgatgttatt gaacgttaat ctgggagata tgatggctca
    37021 ggattttgca gaaaaattga gccgaactaa acctgcctca gtcatcatga ttttagatca
    37081 ttgggaagac ttgcaagaag agctggaagt tgttcagcgt tttgcagttt catacatcta
    37141 taagccagtc cttatcgaaa atctggtagc gcgtatttcg gcgatcttcc gaggtcggga
    37201 cttcattgat caacactgca gtctgatgaa agttccaagg acctaccgca atcttaggat
    37261 agatgttgaa catcacacgg tttatcgtgg tgaagagatg attgctctga cccgccgtga
    37321 gtatgacctt ttggcgacac ttatgggaag caagaaagta ttgactcgtg agcaattgtt
    37381 ggaaagtgtt tggaagtatg aaagtgcgac cgagacaaat atcgtagatg tctatatccg
    37441 ctatctacgg agcaagcttg atgttaaagg acaaaaaagc tacattaaaa ctgtgcgtgg
    37501 tgttggatat accatgcaag aatagaaaag cagttgcagt tgtgtaactg cttttttgag
    37561 gagtttttat atattgacat gcagtcgggt ctttgctaca atcgttttag gaggatatat
    37621 agatgaaact tttgaaaaaa atgatgcaag ttctactagc agtctttttc tttggtttgc
    37681 tagggaccag tacagtattt gcagatgatt ctgaaggatg gcagtttgtc caagaaaacg
    37741 gaagaaccta ctacaaaaag ggggacctca aagaaaccta ctggcgagtg attgatggta
    37801 agtactatta ttttgattct ctatctggag agatggttgt cggttggcaa tacttagaaa
    37861 ttcctggaac aggttatcgt gatgatttat ttgataatcg tccagtcttc gaaattggcc
    37921 ttcaggagaa gtggtactat tttggacaag atggtgcttt gctagaacaa acagataaac
    37981 aagtactaga ggcaaaaacg tctgaaaata caggaaaagt atacggtgaa caatatcctc
    38041 tatctgctga aaagagaact tattattttg ataataatta tgctgtaaag acaggctgga
    38101 tttatgaaga gggtcattgg tattatttaa ataagctagg aaattttggc gatgattctt
    38161 acaatccact accaattggt gaagttgcta agggttggac tcaagatttt catgttacta
    38221 ttgacattga tagaagcaaa cctgctccat ggtactacct agatgcttca ggtaagatgc
    38281 ttacagattg gcaaaaagta aacggaaaat ggtattattt tggctcctct ggttctatgg
    38341 caacaggttg gaaatatgta cgaggcaaat ggtattactt agataataaa aatggtgata
    38401 tgaaaacagg atggcaatac cttggtaaca agtggtacta tcttcgttca tcaggagcaa
    38461 tggcaactgg ctggtatcaa gatggttcaa cttggtacta tttagatcct tctaatggag
    38521 atatgaaaat aggttggaca aaagtaaatg gaaaatggta ttatctcaat tcaaatggag
    38581 caatggttac aggtagtcaa actatcgatg gtaaagttta taatttcgcc tcatccggtg
    38641 agtggattta atattggagg gtatatagat gaaaattttg aaaaaaatga tgcaagctgg
    38701 actaacagta attttctttg gtttgctagc tacgaataca gtatttgcag ataactctga
    38761 aggatggcag tttgtccaag aaaacggtag aacctattac aaaaaggggg acctcaaaga
    38821 aacctactgg cgagtgattg atggtaagta ctattatttt gattctctat ctggagagat
    38881 ggttgtcggc tggcaatata tcccgtttcc atctaaaggt agtacaattg gtccttaccc
    38941 aaatggtatc agattagaag gttttccaaa gtcagagtgg tactacttcg ataaaaatgg
    39001 agtgctacaa gagtttgttg gttggagaac attagagatt aaaactaaag acagtgttgg
    39061 aagaaagtac ggggaaaaac gtgaagattc agaagataaa gaagagaaac gttattacac
    39121 gaactattac tttaatcaaa atcattcttt agagacaggt tggctttatg atcagtctaa
    39181 ctggtattat ctagctaaaa cagaaattaa tggagaaaac tatattggtg gtgaaagacg
    39241 tgcaggttgg ataaatgatg cttcagcttg gtactaccta gatccagcaa ctggtattat
    39301 gcaaacaggt tggcaatatc taggtaataa gtggtactac ctccgttcct caggagcaat
    39361 ggccactggc tggtatcagg aaggtaccac ttggtattat ttagaccacc caaatggcga
    39421 tatgaaaaca ggttggcaaa accttgggaa caaatggtac tatctccgtt catcaggagc
    39481 tatggcaact ggttggtatc aagatggttc aacttggtac tacctaaatg caggtaatgg
    39541 agacatgaag acaggttggt tccaggtcaa tggcaactgg tactatgctt atagctcagg
    39601 tgctttggca gtgaatacga ccgtagatgg ctattctgtc aactataatg gcgaatgggt
    39661 tcggtaatga cagggcgctt ataattatat tagaacactc gcttaatcaa agaagtgact
    39721 ttgttgtact tgacctgacg attggggaga ttaaaagcat gtgaaatcta cacatgcttt
    39781 ttcgttagtt atgagtgttg aacttgacta accattgaca aaagataaga gcaatcaaaa
    39841 taagatacaa aaatatcaat gctaactgca ccgaaacgcc cgatgctatg gatagggcta
    39901 atagggagag cagggacggg gcgagaatgt ctactgtgct cagagcagag cccaccgtcc
    39961 ccatcttgtc actggggata tgggcaaaga cagctgcttg taggcgaggg ctgagaatgc
    40021 cgataatcgt agaactgatg aaactggtca cgaaaattac gatataggct tgacaaagga
    40081 tactagttat tagagccaat agggaaatct cacagaaaac caagaggtgc gtgaaacgga
    40141 tatttttaaa tagattactg ctggtcatat tgccaaggat agctccacaa gacatgatga
    40201 tggagagcag ggcgaagagt tgcccaatgc tcagttgtgc tagtgtctgt tggatgaagc
    40261 ggagggcaat cagccgaggg gacacatcca agatggcaat catactggtc gtcagaaaca
    40321 gtagtatgac ggtatctttt aacctcagga gcacagccag cgactgttta agatgttgaa
    40381 aatatttctt aaaactcagt gctgtatgtg acatttcaat ccttttttca acctcataca
    40441 aggtatgtcg aataacatac aggcccaaaa aggcaatgac aaaagtcaga gtgttgataa
    40501 gggaaatagt ttgaatactt ataacattga taaggaatgc accagccaga ttggcgacaa
    40561 tgcggacaac cctcatcaga gactgcctga accccatggc atcatgcagg tcgtccttaa
    40621 ttacactgat gtagagggcg ttcatcatgt aggcagacag gtagctcaag gtgtcagaga
    40681 tgaggttgat gatgacaatc ataatgactg aaactaatga aaagggcgac aagacgagaa
    40741 atactgtcaa aatagcgtag agcaggattt tgattttggc aatacagagt gcgtgtttga
    40801 ctctattttc ttgaaaatct cctagaatcc cgagaaagag ttgcgatagt aggggaacca
    40861 tctctgaaat ggaaatcagt aaaattgcta gcggagcgaa attgtactga gccacataat
    40921 tgataatggc gaggtagaaa atactgtcgc cgaatgatga aaaacagctg tttagcagca
    40981 actgtctata tatgttgttt gtccaaaata atttcatact tttctcctta tgtctgcaca
    41041 tcgaatggga atttattatt taatagacac caatataccc ccatgcttcc tattccgaag
    41101 tcaaacaaat ctactttacc actatctcct tgaaacatca gtaaaccgtg atcatctcgt
    41161 tcagaacatg ctaaaattac ctgttggatt tttttcatca gtaatttgtt ttgattgtag
    41221 acagtggttt gtagtaagct agttattccg tggcaatatc ctgtcatcat ttttagatga
    41281 tggttaaaaa caaattcttg atatttacta ataatatctt tgtttccgtc acaatcgtac
    41341 atacaaagat ataagattat tccggaaatt ccttcgcacc aagaaagttg taaatttcct
    41401 aaattttcag tattagaagt aactttttct aataattttt ttaattcagc atggaatgta
    41461 tggagatcat tatagaacat agaaggttct aagactttag aataggcaaa taaagcatat
    41521 gcaattccac aatatccatg agcaaagcta tagtctaaaa tgctttcttt ggctgtatct
    41581 ctttgtagga aataatgata aatttcccca tatactattg tggatgaaag ttttaagagt
    41641 attgtcattg gtaaattgat agtattttat taatgataat aggacaccag attttcccaa
    41701 tgcaaagtct atttcctcta gagtatcata attctctatt aatttcttag cggtgacgtt
    41761 tgctaattgt agatagtatt gatttttact gatagaaaat aaatgtagga gtaaccatag
    41821 atagcctgca tcaccaaata gcagtgagta ttgatattca tatgtgtgtg tcgttctaat
    41881 tgtcactgcc ttcttgatga tagagagtat tttagaatcc agttctgggg ggtacaattt
    41941 atttagagga aacagtagtc cagctattcc gtgttggaat gataaattac ttacgaaatt
    42001 tccaaattct gttgaagaag tgatccttcc ttcagaattt acagttttat ctagtaaaaa
    42061 tcgtagaaca ctattacatt ctgttttgta atcatattta tgttcctcta tttttttaat
    42121 tttgactaaa ttatttgttt gacttaaatt tagtagatat tgtaaccaaa atagttgctc
    42181 atctgtgagc tgaccttgag tatgtgatag ttctagtaat tgaataggat cctttaggta
    42241 gattcaatca atctataaca caacaacata ataatacttt taggataact tgtcaaatat
    42301 tatagtaaaa tgaaataa
//
LOCUS       DAVKLX010000012        42156 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKLX010000012 DAVKLX010000000
VERSION     DAVKLX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42156
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.012"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            97..942
                     /locus_tag="VW325_000721"
     CDS             97..942
                     /locus_tag="VW325_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734159.1"
                     /translation="MEVINVSKHYGHSIILKDINFALNKGEIVGLVGRNGVGKSTLMK
                     ILVQNNQPTSGNIISSDNVGYLIEEPKLFLSKTGFENLKYLSNLYGVDYNQERFRSLI
                     QELDLTQSINKKVKTYSLGTKQRLALLLTLVTEPDILILDEPTNGLDIESSQIVLSVL
                     KNLALHENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSAT
                     DRDIFITKQEFGDIVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIY
                     LNRSK"
     gene            958..1686
                     /locus_tag="VW325_000722"
     CDS             958..1686
                     /locus_tag="VW325_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734160.1"
                     /translation="MKLNKLNFLKENIRNLYSSGVIYLGLLISFIPPILVTFFILKTQ
                     GTSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLVFGILVALLLFIPNILNTIMMNTSNDLFIKAIELLPFYSLPVFMASNTMSISQ
                     YLVVITTIILLYFFTLKKSKKYSF"
     gene            2065..2274
                     /locus_tag="VW325_000723"
     CDS             2065..2274
                     /locus_tag="VW325_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9734161.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            2264..2752
                     /locus_tag="VW325_000724"
     CDS             2264..2752
                     /locus_tag="VW325_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734162.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            2868..4328
                     /locus_tag="VW325_000725"
     CDS             2868..4328
                     /locus_tag="VW325_000725"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9734163.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            4325..5149
                     /gene="nadE"
                     /locus_tag="VW325_000726"
     CDS             4325..5149
                     /gene="nadE"
                     /locus_tag="VW325_000726"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9734164.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            5499..6050
                     /locus_tag="VW325_000727"
     CDS             5499..6050
                     /locus_tag="VW325_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9734165.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKVYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            6765..7457
                     /locus_tag="VW325_000728"
     CDS             6765..7457
                     /locus_tag="VW325_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9734166.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYNV
                     DGKSGQVTESTLSQKKDSQTRVVKKRTKPQVLVQEIPIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
     gene            7511..7900
                     /locus_tag="VW325_000729"
     CDS             7511..7900
                     /locus_tag="VW325_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9734167.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(8561..9589)
                     /gene="queA"
                     /locus_tag="VW325_000730"
     CDS             complement(8561..9589)
                     /gene="queA"
                     /locus_tag="VW325_000730"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9734168.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDKWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            9740..10447
                     /locus_tag="VW325_000731"
     CDS             9740..10447
                     /locus_tag="VW325_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9734169.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESNLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            10587..10763
                     /locus_tag="VW325_000732"
     CDS             10587..10763
                     /locus_tag="VW325_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9734170.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            10893..11828
                     /locus_tag="VW325_000733"
     CDS             10893..11828
                     /locus_tag="VW325_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9734171.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            11821..12609
                     /locus_tag="VW325_000734"
     CDS             11821..12609
                     /locus_tag="VW325_000734"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9734172.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGAIVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            12610..12993
                     /locus_tag="VW325_000735"
     CDS             12610..12993
                     /locus_tag="VW325_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9734173.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            13009..13398
                     /locus_tag="VW325_000736"
     CDS             13009..13398
                     /locus_tag="VW325_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9734174.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            13482..14612
                     /locus_tag="VW325_000737"
     CDS             13482..14612
                     /locus_tag="VW325_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9734175.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            14617..15354
                     /locus_tag="VW325_000738"
     CDS             14617..15354
                     /locus_tag="VW325_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9734176.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            15365..16138
                     /locus_tag="VW325_000739"
     CDS             15365..16138
                     /locus_tag="VW325_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9734177.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            16128..16745
                     /locus_tag="VW325_000740"
     CDS             16128..16745
                     /locus_tag="VW325_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9734178.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            16955..17356
                     /locus_tag="VW325_000741"
     CDS             16955..17356
                     /locus_tag="VW325_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9734179.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            17356..17634
                     /locus_tag="VW325_000742"
     CDS             17356..17634
                     /locus_tag="VW325_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9734180.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            17624..18397
                     /locus_tag="VW325_000743"
     CDS             17624..18397
                     /locus_tag="VW325_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9734181.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            18398..19702
                     /locus_tag="VW325_000744"
     CDS             18398..19702
                     /locus_tag="VW325_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9734182.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            20276..21154
                     /gene="pstS"
                     /locus_tag="VW325_000745"
     CDS             20276..21154
                     /gene="pstS"
                     /locus_tag="VW325_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9734183.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            21154..22071
                     /gene="pstC"
                     /locus_tag="VW325_000746"
     CDS             21154..22071
                     /gene="pstC"
                     /locus_tag="VW325_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9734184.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            22061..22945
                     /gene="pstA"
                     /locus_tag="VW325_000747"
     CDS             22061..22945
                     /gene="pstA"
                     /locus_tag="VW325_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9734185.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGIYLHKKLTAA"
     gene            22956..23759
                     /gene="pstB"
                     /locus_tag="VW325_000748"
     CDS             22956..23759
                     /gene="pstB"
                     /locus_tag="VW325_000748"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9734186.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            23772..24530
                     /gene="pstB"
                     /locus_tag="VW325_000749"
     CDS             23772..24530
                     /gene="pstB"
                     /locus_tag="VW325_000749"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9734187.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLNPQHKETEDYITGKFG"
     gene            24542..25195
                     /gene="phoU"
                     /locus_tag="VW325_000750"
     CDS             24542..25195
                     /gene="phoU"
                     /locus_tag="VW325_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9734188.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            25352..26167
                     /locus_tag="VW325_000751"
     CDS             25352..26167
                     /locus_tag="VW325_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9734189.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(26187..27458)
                     /locus_tag="VW325_000752"
     CDS             complement(26187..27458)
                     /locus_tag="VW325_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9734190.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            27600..28331
                     /gene="budA"
                     /locus_tag="VW325_000753"
     CDS             27600..28331
                     /gene="budA"
                     /locus_tag="VW325_000753"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9734191.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            28341..29093
                     /locus_tag="VW325_000754"
     CDS             28341..29093
                     /locus_tag="VW325_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9734192.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            29205..30110
                     /gene="murB"
                     /locus_tag="VW325_000755"
     CDS             29205..30110
                     /gene="murB"
                     /locus_tag="VW325_000755"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9734193.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            30271..31428
                     /locus_tag="VW325_000756"
     CDS             30271..31428
                     /locus_tag="VW325_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734194.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEKRDEENKL"
     gene            31409..32215
                     /locus_tag="VW325_000757"
     CDS             31409..32215
                     /locus_tag="VW325_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734195.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWIMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSAIGVILILTMFITMWVTKERRER"
     gene            32212..32985
                     /locus_tag="VW325_000758"
     CDS             32212..32985
                     /locus_tag="VW325_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734196.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGNDMNSFTGFSLSH
                     FKTMFGDGCLMLIVTQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            32982..34052
                     /locus_tag="VW325_000759"
     CDS             32982..34052
                     /locus_tag="VW325_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9734197.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLEQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDLGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKSEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNAAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDA
                     AYAFINFMLKPENALKNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEIYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            34313..34552
                     /locus_tag="VW325_000760"
     CDS             34313..34552
                     /locus_tag="VW325_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734198.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            34827..35312
                     /locus_tag="VW325_000761"
     CDS             34827..35312
                     /locus_tag="VW325_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9734199.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            35334..37952
                     /gene="alaS"
                     /locus_tag="VW325_000762"
     CDS             35334..37952
                     /gene="alaS"
                     /locus_tag="VW325_000762"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9734200.1"
                     /translation="MKQLSSAQVRQMWLDFWATKAHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAAILKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(38042..39496)
                     /locus_tag="VW325_000763"
     CDS             complement(38042..39496)
                     /locus_tag="VW325_000763"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9734201.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            39675..40424
                     /locus_tag="VW325_000764"
     CDS             39675..40424
                     /locus_tag="VW325_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734202.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELLASSY
                     ELSSSDLEASLARLLSVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDNPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            40405..42039
                     /locus_tag="VW325_000765"
     CDS             40405..42039
                     /locus_tag="VW325_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734203.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLFMFINIAFRFPFEEMPSFFSTMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLLLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKDRISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPILTLLGLSLYLGLPIISMIYLIMAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLST
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
BASE COUNT        12152 a         7837 c         9535 g        12632 t
ORIGIN      
        1 tagatatatg atataatgtt gttaaaaaga ggcgcaactt tttaaactca ataagaatca
       61 aagagcaaac caagaatatt aatggaggaa taaaaaatgg aagttataaa tgtaagtaag
      121 cattatggtc attcaatcat tctcaaagat ataaattttg cacttaacaa gggtgaaatt
      181 gttggtctag tagggagaaa tggagttggt aagagtacgt tgatgaaaat tcttgttcag
      241 aataatcaac cgacttcagg gaatattata agcagtgata atgttgggta tttaattgaa
      301 gaaccaaaat tatttttatc taaaacaggt tttgagaatt taaaatattt gtcaaattta
      361 tatggtgttg actacaatca agaaagattt agaagtttga ttcaagagtt agatttgact
      421 cagtctatta ataaaaaagt aaagacctat tctttgggta caaaacaaag attagctttg
      481 cttctaactc tcgttacgga acctgatata ttgattttag atgaaccgac taatggttta
      541 gatattgaat catcacaaat agttttatcg gttctaaaaa atttagcttt acatgaaaat
      601 gtgggaattt taatatcgag tcataaatta gaagatattg aagaaatttg tgagagggtt
      661 cttttcttgg agaacgggct tttgacattt caaaaagtag gaaaagatag tcataatttc
      721 ttgtttgaga tagctttttc atcagctaca gatagagaca ttttcattac caaacaagaa
      781 tttggggata ttgttcagga agagggactg agaattacta tgtctgggaa tattcaaagt
      841 agtgagcttt ttaaattttt taacgaaaac tctattaaag tagttgattt tgaaactaaa
      901 aaagagacgc ttaaagatat ttatctaaat cgttcaaaat aaaggaaggt tataatcatg
      961 aaattaaata aattgaattt tcttaaggaa aatataagaa atttatattc atcaggcgta
     1021 atatatctcg gtttgcttat ctcgtttata ccgccgatat tggttacatt ctttattcta
     1081 aagactcaag ggacatcgct tggtattaag catatttcaa acttttatgc tatgctcggt
     1141 atgttaatgg ctgttataca tgctaatcga gtcattagta gagatttttc ccacaatacg
     1201 gtaagtttgt tttataatca acagaagaat cggatgattt acgtcttgtc taattttcta
     1261 tatgccatct cagtttccat tatttatgct ttgaatggca ttgtgttact agtcatagta
     1321 agtaaattgg gtattccagg tgatttagga ttagatttta tagtagctat tgtagtcaat
     1381 acaattttgt tagtcctgtt ttattttcta ttatcttaca ttttctattt atacaaattg
     1441 aaaagtggct tggtatttgg tattttagta gctttactac tctttatccc taatatatta
     1501 aatacgatta tgatgaatac tagtaatgat ttgtttatca aagcaattga acttcttcct
     1561 ttttattctt tacctgtatt tatggcttca aatacgatgt ctattagtca gtatcttgtg
     1621 gtaatcacta caatcatttt attgtacttt ttcactctca agaaaagcaa gaagtattca
     1681 ttttagtttc atttagtatt attttgcata cctaaaatac agtaaaaaat cggtcatctt
     1741 ggtatgctcc tgctcttgtt gtgtacacgt ttttgtctta tgctagactc atttccaaaa
     1801 gcattatata atagtgatat gaaatcaact aaataagaaa tacaagcaat caaaactcgt
     1861 ttaaaagacc ctactaaagc taatactaaa taaaaataaa agagtaaact aggaagttta
     1921 tttcaaacaa cctaaaatac tgattttcgg ctgaagataa tactggagtg caaattaatg
     1981 gggttataat aaatagctga tagcttgtgt tggttttgga ttttttaaga gtagatgagt
     2041 attaaaacta taaggaggac gaaggtggct aaaaatttaa aattaaaatt agctcgggta
     2101 gagcgtgatt taacacaagg tcaactggca gaggctgtcg gggtgacacg ccagactatt
     2161 ggtttgatag aggcgggaaa atacaatccc agtctctcgc tctgccagtc tatttgcaga
     2221 tgtttaggga aaaccctaga ccaactattt tgggaggaag aagatgaaaa atagatttta
     2281 ttattatcaa ttactagacg aaagagaaga acaactgttc aataaagcgg gctctgaaag
     2341 tttctatatc tgcattgctt tgtcgctcct atcttatatc atttcagtat tagcaccaag
     2401 cctttttaat tctaatatgc tgctaatcgt tatcatcata gggacatttt actttttcaa
     2461 tcgtgcccgt tatctgggag tgacctacta tagtcgtttt cattttacga ttttgggttg
     2521 ttttttccta accttggcta ttacggctct tttgatgttg cagaattatc aattcaacat
     2581 agaaatttat cagcacaatc ctttgaattt taaatacctg tctgcttggg tcattactta
     2641 tgtcatttac cttccgtggg tctttattgg caatcttggt cttaagagct atggcgaatg
     2701 ggctcagaaa aaatttgaac aagatatgga tgaattggag agtggagaat agcttgttac
     2761 tcttttctca atccagctaa aatgtgatat aatagtacta atttattgga atacatgaaa
     2821 gttcttgaaa attttcatgg gtttctagct aaggaagtag gaaaagtatg tatccagatg
     2881 atagtttgac attgcacacg gacttgtacc agatcaacat gatgcaggtt tactttgacc
     2941 aagggattca caataagaag gcggtctttg aggtgtattt ccgccaacag ccttttaaga
     3001 acggctatgc ggtttttgca ggtttggaaa gaattgtgaa ctatcttgaa gacttgcgtt
     3061 tttcagatag tgatatagcc tatttggagt cgcttggtta tcatggggcg ttcttggatt
     3121 accttcgcaa tttcaagttg gagttgaccg ttcgttctgc ccaagaaggg gatttggttt
     3181 ttgctaatga accgattgtg caggtggaag gacctctagc ccaatgtcag ttggtcgaaa
     3241 cggctctttt gaacatcgtc aactaccaga ccttggtggc gacgaaggca gctcgtattc
     3301 gttcggttat cgaagatgaa cccttgatgg agtttgggac acgtcgggct caagaaatgg
     3361 atgcggccat ctggggaaca cgcgcagcgg tgattggtgg cgccaatgga accagcaacg
     3421 tgcgtgcggg taagctcttt gacattcctg ttttgggaac ccatgcccat gccttggtac
     3481 aggtttatgg aaatgactat gaagctttca aggcttacgc tgcgacccac aaaaattgtg
     3541 tctttcttgt ggatacctat gacacccttc gcatcggtgt accagctgcc attcaggtgg
     3601 cgcgtgagct gggtgatcag attaacttta tgggtgtgcg gattgactct ggggatattg
     3661 cctacatttc taagaaagtc cgtcagcaac tggacgaggc tggatttaca gaggctaaga
     3721 tttatgcttc taatgatttg gacgaaaata ctatcctcaa tctcaagatg caaaaggcca
     3781 agattgatgt ctggggcgtg ggtaccaagc tgattacagc ttatgaccag ccagctcttg
     3841 gtgcagttta caagattgtt gcaatcgaag atgaaactgg tcagatgcgc aatacgatta
     3901 agctgtctaa taatgctgaa aaagtgtcga cgccaggtaa gaagcaggtg tggcgcatta
     3961 ccagtcgtga aaaaggcaag tcagaaggcg actatatcac ttatgatggt gtggatatta
     4021 gcgacatgac agaaatcaag atgttccatc cgacctatac atacatcaag aagacggttc
     4081 gtaattttga tgccgttcct ctcttggtgg atatcttcaa agaaggaata ttagtttaca
     4141 acttgcctag tttgactgac attcaggatt atgcccgtaa ggaatttgac aagttgtggg
     4201 atgagtataa gcgtgtgctc aatccgcagc actatccagt ggatttggcg cgtgatgtat
     4261 ggcaagataa gatggacttg attgataaga tgcgcaagga agcccttggt gaaggagaag
     4321 aagaatgagt ttgcaagaaa cgattatcca agagctgggt gtcaaaccag tgattgatgc
     4381 ccaggaagaa atccgtcgtt ctattgattt cttaaaaaga tatctgaaaa aacatccctt
     4441 cctaaaaacc tttgtactag ggatttctgg gggacaagac tcaaccttgg cagggcgttt
     4501 ggcgcaatta gctatggaag aactgcgagc tgaaacggga gacgacagct acaaatttat
     4561 cgctgtccgc ctgccatacg gagtgcaagc tgatgaagca gatgctcaaa aagccctagc
     4621 cttcatccag ccagatgtca gcttggttgt gaatatcaag gaatcagctg atgccatgac
     4681 agctgcagtt gaagcgacag gtagtcctgt ttcagacttc aacaagggga atatcaaggc
     4741 acgttgccgt atgattgctc agtatgccct tgctggttcc catagcggag cggtcattgg
     4801 aacagaccac gccgcggaaa atatcacagg tttctttacc aagtttggtg acggcggtgc
     4861 ggatattctc cctctttacc gcctcaataa acgccaagga aaacagctct tgcagaaact
     4921 tggcgcagag ccagcccttt atgaaaaaat cccaacggca gacctagaag aagataaacc
     4981 aggcctagct gacgaagtcg cacttggagt cacctacgca gagattgacg actacctaga
     5041 aggcaaaaca atcagcccag aagctcaagc gaccattgaa aactggtggc acaaaggcca
     5101 acacaaacgc cacttaccca tcaccgtatt tgatgacttt tgggagtaaa aaggtccggg
     5161 ggaccttttt agcttcttgc cctgaaatta aaaagcaaga aaaacctcca ctggaggttt
     5221 tcagcctctc atcttgaaat aagaaagtga gagaaggtcc gggggatctt gaaccccgag
     5281 tttagaaata agaaaatgag gcagattcgg taactcgaag agttcgattt catcgtctta
     5341 cccctgcaac gatgactagg tttgaaaaag attgctagag cgcatttcaa accaggcagc
     5401 gactgcgtca agaaattaga agacaaactc gttttctagc tgttactgag ttgagtcttt
     5461 ttactacgag catagaaata aggaagtgag gtagcatcat gaaatctatc ggtacgcaaa
     5521 tattacagac agaacgtttg attttaagaa gatttgtgga gagtgatgca gaagctatgt
     5581 ttcaaaattg ggcttcatcc gctgagaatt tgacctatgt tacctgggat ccccatcctg
     5641 atgtcgaaat cactcgaaac tcgattcgca attgggttgc ttcctatact aatctcaact
     5701 attacaaatg ggccatttgt ctaaaagaaa acccagagca agtgatagga gatatcagca
     5761 tcgttaagat agacgaggct gatttaagct gtgaaatcgg ctatgtgcta ggcaaggttt
     5821 actggggaca tggtatgatg acagaggcct tgaaagctgt cttggacttt tgttttactc
     5881 aagcaggttt tcaaaaggtc agagcacgtt atgccagtct caacccagct tcaggtcgtg
     5941 tcatggaaaa ggctggaatg tcctatctac aaaccattgt taatggtgta gagagaaaag
     6001 gctatcttgc ggatcttatt tattatggta taagtaggga agaatgttga attctatttt
     6061 ctgtttctat cgaagtcaac tatttattgt aaatataata attagcattc caagtttatt
     6121 tgcaatttta aaatagcata ttgattagta caagacagat gttctagttc cttctttaat
     6181 ctggtttagt gttagttaaa aaatcgcttt aagcttgtaa ctaagaggga gctaatcgac
     6241 tagattctcc agccgaacag gtggtaatgt actttttata gtgtaatcct agctgttgtt
     6301 aaatttaaaa tagaatcctc tatcgagtta gggaattaaa ttcaaccaat tttattcatg
     6361 ttttttctat caaattatct aatattaaaa tagtctcatt ctgatgagaa aactattcta
     6421 tttatactgc atagttgtat aaggaatgat gaaagatgag tgaaaattat caagttggaa
     6481 tgtttgtatc taaatatatt agcatgtatt tagataagat gtctgcaatc ctttatatat
     6541 gagattgatt acgatatagt taaagtgtta gttcaataat caatatatca ttctaagtct
     6601 attttatata taaagtatat tgtttaacgt ttgatagaaa agatgctata ctagaagtag
     6661 gatgtaatgg caaaatctaa tactaataag cgctatgcaa tacgtaaact cggaaagatt
     6721 tttggttctg cggtaatcgg gctaacttta tttttaggag gaatctgaat acaagttttg
     6781 ttcatgctgc tgatgggatt caatatgtca gagatgatac tagagataaa gaagagggaa
     6841 tagagtatga tgacgctgac aatggggata ttattgtaaa agtagcgact aaacctaagg
     6901 tagtaaccaa gaaaatttca agtacgcgaa ttcgttatga aaaagatgaa acaaaagacc
     6961 gtagtgaaaa tcctgttaca attgatggag aggatggcta tgtaactacg acaaggacct
     7021 acgatgttaa tccagagact ggttatgtta ccgaacaggt tactgttgat agaaaagaag
     7081 ccacggatac agttatcaaa gttccagcta aaagcaaggt tgaagaagtt cttgttccat
     7141 ttgctactaa atatgaagca gacaatgacc tttctgcagg acaggagcaa gagattactc
     7201 taggaaagaa tgggaaaaca gttacaacga taacttataa tgtagatgga aagagtggac
     7261 aagtaactga gagtacttta agtcaaaaaa aagactctca aacaagagtt gttaaaaaaa
     7321 gaaccaagcc ccaagttctt gtccaagaaa ttccaatcga aacagaatat ctcgatggcc
     7381 caactcttga taaaagtcaa gaagtagaag aagtaggaga aattggtaaa ttactcttac
     7441 tacaatctat actgtagatg aacgtgatgg aacaattgaa gaaactactt ctcgtcaaat
     7501 tactaaagag atggtaaaaa gacgtataag gagagggacg agagaacctg aaaaagttgt
     7561 tgttcctgag caatcatcta ttccttcgta tcctgtatct gttacatcta accaaggaac
     7621 agatgtagca gtagaaccag ctaaagcagt tgctccaaca acagactgga aacaagaaaa
     7681 tggtatgtgg tatttttata atactgatgg ttccatggca acaggttggg tacaagttaa
     7741 tggttcatgg tactacctca acagcaacgg ttctatgaaa gtcaatcaat ggttccaagt
     7801 tggtggtaaa tggtattatg taaatgcatc gggtgagtta gcggtcaata caagtataga
     7861 tggctataga gtcaatgata atggtgaatg ggtgcgttaa cagattcctc cctgcgacaa
     7921 aattgataat agtatgtctg aatatataat tatatataaa aagccataga taaggtcatc
     7981 ttatagcgac tataaaaaag tgaacaagcc tagtcttctg tacattagaa ttcagatttg
     8041 ttcacttttt gtttaatact caatgaaaat caaagagcaa actagaaaac tagtcgcagg
     8101 ttgctcaaag tactgctttg aggttgtaga tagaactgac gaagtcagtc acatatataa
     8161 tccaaggtga cgttgacgcg gtttgaagag atttttgaag agtataagtc tttgattttg
     8221 agactaagga gagtcagggt caaagtagtg aatgattctg aaataactca taatagtctc
     8281 ttgagaggac agataagagt aacaaatatg gtgctactca tgtgtcttgc attttgattt
     8341 acaaacagcg aagaaaatat aggaagctga aataaaatat agatgaatca atttggagag
     8401 tcaacgcaat ctatggcaat attttcgaag ctagggggaa gcattttaat ttcaatgtac
     8461 taggcgtctt ctacagctag ctagagtttc aattgtagat gttctttgaa ataatatctt
     8521 agcgatttat tggtattaga agaattagag aaattctttc tcaataaata aacatggcgt
     8581 caccaaaact gaagaagcgg tagtgttctt ggatggcatg gtggtaggca tctaagacta
     8641 attcacggcc tgcaaaggca gaaaccaaca tgaccagagt tgattttggc agatggaagt
     8701 tggttgagaa ggcatccacg accttccact catacccagg tttgataaag atattggtcc
     8761 aaccagaatc tgcttggatt tgcccatcaa acttggaacc aatagtttcc aaggtgcgga
     8821 tagaagtggt tccgacagcg atgacgcgac caccattttc tttgacagag cgaagggtgg
     8881 cagcagcttc ctcagaaagt tgatagaact ctgagtgcat ttcgtgttcg tccagattat
     8941 ccacagaaac aggtctaaag gttccgagtc cgacatggag agtcagatag actagatgaa
     9001 cacccttagc ttggatttct gccagcagtt ctttggtgaa gtgaagacca gcagtcggtg
     9061 ctgcagcaga gccgctttcc ttggcgtaga cggtttgata acgttcacgg tcatctaatt
     9121 tttcgtggat ataaggtggc agaggcattt ctcccagact ttccaagact tctaggaaaa
     9181 ttccttggta ttcaaagcgg acaatgcgtc ccccgtgggt caattcctct gtaacgacag
     9241 cgctgaggcg gccatcacca aagctgatac gagtaccgac cttgaggcgt ttggcaggtt
     9301 tagccagaac ttcccacttg tctccactag tgttcttaag gaggagaagt tccacatgac
     9361 ctcctgtctc cactttttga ccatagaggc gggcagggag aactcgggtg tcgttcatga
     9421 caagggcatc accaggttcc agcatatcaa taatagagtg gaagtgttta tcttgcattt
     9481 ctcctgtctc acggttgacg atgaggagtt tggaggcatc acgtttttca aggggcgttt
     9541 gggcaatcaa ttcctctggc aagtggaaat caaaatcagc tgtattcata tatctgttca
     9601 ttctttctaa gttctaatcc tatccattat aacacgtttc aagtttggac gctctttttt
     9661 tagaaattaa atcgttttca cttgacaaaa attggtctat accatataat aaatatagat
     9721 agaaatggag gatgaaaaga tgaaagttat taaagttgaa aaccaagttc aaggtggaaa
     9781 agttgctttt gagattttga aggaaaaatt ggctaatgga gctcaaactc taggacttgc
     9841 gacaggaagt agcccacttg aattttacaa ggaaattgtt gagagtaacc ttgatttttc
     9901 aaatctaacc agtgtcaacc ttgatgagta tgtgggcctt gatggggaca atccacagtc
     9961 ttatcgttac ttcatgcaag aaaacttgtt caaccaaaaa ccatttaaag aaagtttctt
    10021 gcctcgtggt gttaaggaca atgctgaagc tgaagttgaa cgctacaacc aaattttggc
    10081 tgaccatcca gttgacctcc aaatcttggg aatcggtcgc aatgggcata tcggctttaa
    10141 tgagcctggt actccatttg acagtcaaac gcatctagta gaacttgacc agtctactat
    10201 tgaagccaat gcacgcttct ttgccaagat tgaagacgtc ccaacccaag ctatttcaat
    10261 ggggattaaa aacattttgg atgccaagtc tattattctt tttgcttacg gtgagtcgaa
    10321 agcagaagcc attgccggaa cagtatctgg cccagtgact gagaacctac ctgcaagtag
    10381 cctccaaaat caccctgatg taaccatcat cgcagatgct gaagcgctta gcttactcga
    10441 aaaataaaaa agcaatgttc tattgaacgc tttcaactct tgtataaatg gaagaaaaaa
    10501 tcaaaattaa accgcatttt tgcttgacaa ttattccttt tacgtgtaga atgaaataga
    10561 tattgaactt gaagggagtg aaaaaaatgt ctaaaacagt agtacgtaag aatgaatctc
    10621 ttgacgacgc acttcgccgt ttcaaacgtg cggttactaa agctggtact cttcaagaaa
    10681 cacgcaaacg tgaattctat gaaaaacctt ctgtaaaacg taaacgtaaa tcagaagtag
    10741 ctcgtaaacg taaaaaattc taattggaat gaaaggctag agaaatctag tcctttttct
    10801 tttaaataaa tactccaaag cctgcaaaaa tctgaaactt cctcctacaa tttgatataa
    10861 tagagagaag aactcatttg aaggaggaaa tgatgtcggt tttagtaaaa gaagtgattg
    10921 aaaagcttag actagatatt gtctatggtg aaccagagtt gcttgaaaag gaaatcaata
    10981 cagcggatat tacgcgacct ggtcttgaaa tgacaggcta ttttgactac tatacaccag
    11041 agcggattca acttttgggg atgaaggagt ggtcttatct gatcagcatg ccttccaaca
    11101 gccgttatga agttttgaaa aaaatgtttc tacctgagac accagcagtc attgttgccc
    11161 gtggtttggt ggttccagag gagatgttaa aggctgctag agaatgtaag attgctattt
    11221 taaccagccg tgcagctacc agtcgtttat ctggagagtt atctagctat ctggattctc
    11281 gtttggcaga acgtaccagt gtgcacggtg tcttgatgga tatttatggg atgggcgtct
    11341 tgattcaggg agatagtgga attggtaaga gcgagacagg tcttgagctt gtcaaacgtg
    11401 gtcaccgttt ggtagccgat gaccgtgtcg atatctttgc caaggatgag attactctct
    11461 ggggtgaacc agctgaaatt ttgaaacact tgattgaaat tcgtggggtt ggtattatcg
    11521 atgttatgag tctctacggt gcgagtgctg tcaaggattc ttcacaggtt cagcttgctg
    11581 tctatttgga aaattacgat acgcataaga cctttgatcg tcttggaaac aatgcagagg
    11641 aacttgaagt ttctggcgta gccattcctc gtattcgtat tccagttaaa acaggtcgta
    11701 atatctctgt tgtgattgag gcagctgcca tgaattatcg tgccaaggaa atgggctttg
    11761 atgcgacccg tttgttcgac gaacgactga caagtctcat cgctcgaaat gaggtgcaaa
    11821 atgcttgatc caattgctat tcaactagga cccctagcca ttcgttggta tgccttatgt
    11881 attgtgacag gcttgattct tgcggtttat ttgaccatga aagaagcacc tagaaagaag
    11941 atcataccag acgatatttt agattttatc ttagtagcct ttcccttggc tattttagga
    12001 gctcgtctct actatgttat tttccgattt gattactata gtcagaattt aggagagatt
    12061 tttgccattt ggaatggtgg tttggccatt tacggtggtt tgataactgg ggctcttgtg
    12121 ctctatatct ttgctgaccg taaactcatc aatacttggg attttctaga tattgcggcg
    12181 cctagcgtta tgattgctca aagtttgggg cgttggggta atttctttaa ccaagaagct
    12241 tatggtgcaa tagtggataa tctggattat ctacctggct ttatccgtga ccagatgtat
    12301 attgagggga gctaccgtca accgactttc ctttatgagt ctctatggaa tctgcttggc
    12361 tttgccttga ttctgatttt tagacggaaa tggaagagtc tcagacgagg tcatatcacg
    12421 gccttttact tgatttggta tggtttcggt cgtatggtca tcgaaggtat gcgaacagat
    12481 agtctcatgt tcttcggcct tcgagtgtcc caatggctgt cagttgtcct tatcggtctc
    12541 ggtataatga tcgttattta tcaaaatcga aagaaggccc cttactatat tacagaggag
    12601 gaaaactaaa tgttagaagt tgcatatatt cttgttgccc tagctttaat tgtctttttg
    12661 gtctatctga tcattactgt acaaaagctt ggtcgtgtca tcgatgaaac agaaaagacg
    12721 attaaaacct tgacttcaga tgtggatgtg accttgcatc acaccaatga gttgttggct
    12781 aaggtcaatg tcttggcaga tgatatcaat gtcaaggtgg ctacgattga tccactcttc
    12841 agtgctgttg cagatttatc tctatctgtt tcagacctca atgaccatgc gcgtgtcttg
    12901 agcaagaaag cttcatcagc tggttcaaaa acactcaaga ctggtgcaag tctgtcagct
    12961 cttcgtcttg caagtaaatt tttcaaaaaa taaaaaagga gaatccttat gggtaaatta
    13021 tcctcaatcc ttttaggaac ggtttcaggt gcagctcttg ccttgttttt aacaagtgat
    13081 aagggcaaac aagtttgcag tcaggctcaa gattttctag atgatttgag agaagatccg
    13141 gagtatgcca aggagcaagt ctgtgaaaaa ctgacagaag ttaaggagca ggctacagat
    13201 tttgttctga aaacaaaaga acaggttgag tcaggtgaaa tcactgtgga cagtatactt
    13261 gctcaagcta aatcctatgc ttttcaagcg acagaagcat caaaaaatca attaaataat
    13321 ctcaaggaac aatggcaaga aaaagccgaa gctcttgatg actcagaaga gattgtgatt
    13381 gatataacag aagaataaac catcaccatc tccggacgga ctatgtatct ggggatggtg
    13441 atttttatct ggaatctagt ctttgtggta taataattac tatgcagaaa aaaccaacgt
    13501 cagcctatgt gcacatccca ttttgtaccc agatttgtta ttattgtgat ttttcaaagg
    13561 tcttcatcaa aaatcagcca gtcgacagct atttagagca tctgctggaa gagtttcgtt
    13621 cttatgatat tgaaaagttg tcaacccttt atatcggtgg tggaacaccg acagccctgt
    13681 cggctccgca actggaggtg ttactgaatg gcttgactaa aaacttggac ttgtctgtct
    13741 tggaagagtt gaccattgaa gccaatccag gcgatttgga tgcggataag atagctgttt
    13801 tgaaaaattc ggctgtcaat cgtgtttcgc taggtgtcca gacctttgat gataagatgt
    13861 tgaaaaagat tgggcgcagt catttggaga aggatattta tgaaaatatc gatcgtctga
    13921 aactggctgg ttttgacaac atctccattg atttgattta tgctctgcct ggtcagacca
    13981 tggagcaagt aaaggaaaat gtggctaaag ccattggact ggatattccc cacatgagtt
    14041 tgtatagttt gattttagaa aaccatacgg tctttatgaa ccggatgcga cgtgggaaat
    14101 tgcctctgcc taaggaggaa ctagaagcgg agatgtttga gtacatcatt gcagagctgg
    14161 agcgagcggg ttttgagcat tatgagattt ctaatttctc caaacccggt tttgaaagtc
    14221 gtcacaatct catgtactgg gacaatgctg aatactatgg tattggtgca ggggcatctg
    14281 gttatgtcaa tggagtacgc tataaaaatc atggtcccat tcgtcattat ctcagtgcgg
    14341 ttgaggaagg caatgcttgt attacagaag atcacctgag tcaaaaggag caaatggaag
    14401 aagaaatgtt cttgggactc cgcaagaaat ccggggtttc catggcgcga tttgaggaaa
    14461 aatttggaca gtcttttgct ggactttatg gagaaattgt cagagatttg gttcaacaag
    14521 gcctcatgca gattgagggt gaccacgttc gcatgacaaa gagaggtctc tttttgggag
    14581 atactgtagc agaacgattt attttggagt aggatcatgg gcttaactta tcaaatgaaa
    14641 atgaaaattc cttttgatat ggctgatatg aacggtcata tcaaacttcc agatgtgatt
    14701 ttgctgtcac tgcaagtttc agggatgcag tcgattgaac tgggagttag tgataaggcc
    14761 attttggaag aatataatct ggtctggatt atcgtagaat atgatattga ggtggttcgt
    14821 ttgcctcgtt ttgcggaaga aattaccatc gaaacggaag ccttgagcta caatcgactt
    14881 ttttgctacc gtcgctttac tatttataat gaagcggagc aggagctcat ccacatgatg
    14941 gcgacctttg ttctcatgga ccgcgacagt cgaaaagtcc atgttgttga acctgagatt
    15001 gtggctcctt accagtctga ttttgataaa aaacttatcc gtggaccgaa gtatgagtcc
    15061 ttggaagaac cgatcagcaa ggattaccat gttcgttttt acgacttgga tatgaatggt
    15121 catgtcaata acagtaaata cttggactgg atttttgagg tcatgggagc ggattttttg
    15181 acccactata ttcccaagaa aatcaacctc aagtatgtca aggaagttcg accaggtggg
    15241 gtgattacat cggctgttga acggactgga ctggaaagca agcatgaaat tacaagtgat
    15301 ggggttatca atgcccaagc aatcattact tggcaagaaa taaaaaaggc ttagggagaa
    15361 atagatgaaa tataaaggct atttaattga tttagacgga accatttata aggggaaaga
    15421 ccgaatccct gctggagaga cttttgttca tgaattgcaa aagcgggaca ttccctatct
    15481 ctttgtaacc aacaatacaa cccgcactcc agagagtgtt aaggagatgt tggctcagaa
    15541 ttttaatatc gatacgcccc tatcgactgt ctacacagcg actttggcaa ccatcgacta
    15601 tatgaatgat ttggggcttg aaaagaccgt ctatgtcgtc ggagaagcag gactcaagga
    15661 agccatcaag gcggctggtt atgtggaaga caaggaaaaa cctgcctacg tggtagttgg
    15721 tctggattgg caagtcgact atgaaaaatt tgccacagca actcttgcta ttcaaaaggg
    15781 tgcccatttt atcggaacca atcctgacct caacatcccg acggaacgcg gtcttttgcc
    15841 aggtgctggc tcactgatta cgctgcttga agtagcgaca cgagtgaagc cggtttatat
    15901 cggaaaacca aatgccatca ttatggacaa ggcggttgag cacttaggtt tggaacgtga
    15961 agagttaatc atggttgggg acaattattt gactgatatc cgggctggga ttgacaatgg
    16021 cattccaacg ctcttggtga cgacaggttt taccaaggca gaagaagtag ctggcctgcc
    16081 aatcgcaccg acacatgtgg tttctagcct tgcggagtgg gattttgatg aaaactaaat
    16141 tgaccttttg gggctctatg ctctttctcc tctccctctc aatccttctg accatttatc
    16201 tggcttggat tttctatcct atggagattc agtggctaaa cttaacgaat cgagtctatc
    16261 taaaaccaga aaccattcaa tacaattttc atatcttgat gaattatctg accaatcctt
    16321 ttagtcaggt cttacagatg cctgattttc gttcgtcagc agctggtctg caccatttcg
    16381 cagtggtcaa gaatctcttt catttggttc agctagtagc tctagtgaca ctgccaagtt
    16441 tctatgtctt tgtcaatagg attgtgaaaa aggacttttt gtctctttat cgaaaaagtc
    16501 tcctggctct agtagtctta cctgtgatga ttggacttgg gggagttttg attggttttg
    16561 accaattctt tactcttttc catcaaattc tctttgtggg agatgatacc tggctttttg
    16621 atccagccaa ggatcctgtt attatgattt tgccagagac cttctttctt catgccttcc
    16681 tcctcttttt tgccctctat gaaagcttct ttggctatct gtgtctgaaa agtcgtagga
    16741 agtgaaatga aagatgtttt taatttgcat atataaaaat atatatcgtt taaaaccatc
    16801 gttaagagtg aattaaccaa atccaattgt gtcaatcgtt cgcaaacagc ctatttttct
    16861 tgaaaaaata ggcttttttt ctaaaaatcc ctagtcaagc gccttatttt tttgtataat
    16921 agaagtagca aagtatagat aagaagagaa aagcatgatt acactatttc tatcaccgag
    16981 ctgtacatca tgtcgtaagg ctaaggcctg gttagaaaaa cataaggttc cctttgtgga
    17041 acacaatatt atgaccagtc ctttaacaag aaaagaattg caacacatcc tttccttgac
    17101 cgaaaatggt actgatgaca tcatttcaac tcgttcaaaa atttttcaaa aattgaatat
    17161 tgatgtagag agtatttcgg tatcggaatt gcttcatttg attgagcagt atcctagtct
    17221 tttgcgtcgt ccaattatta ttgatgccaa gcgtatgcaa atcggtttta atgaagatga
    17281 gattcgtgct tttctccctc gtagttatcg taagcaagaa ctaaaagaag caagaatgag
    17341 agctggtatt agttaatgaa caaacagtat agttacccac tagatttgtc gtggagcact
    17401 gaagaacttg cttcagtgct ttcttttttt aatgatgttg aagctgccta tgaaggcaaa
    17461 gtagaggcta aaaagttact ggactcctat aagggattca aagcggtcgt gccaagcaag
    17521 agcgaagaga aacgtttggg acgagaattt gaaacggtta gtggctattc actttaccga
    17581 gcagttcagg ctgccaaaga aaaaggggaa ggaaagattt ctcttggaaa gtaaatttga
    17641 atttgctaaa gagcttgtta agaaagcagg tcagtacatt cttgaccata tgcaggaaga
    17701 cttgcgtgtt gaaaccaagt cctctccaac agatttggtg accagactgg acaaagaagt
    17761 tcaggaactc ttggttggtg agattttgtc ccgttatcct gaggataaga tttgtgctga
    17821 agaaggttgt ctgcgagcct cggttcaaga gggcaaggtt tgggtcattg atcccattga
    17881 tggtaccaat aattttgtag cccagcagga agattttgct gttatgatgg cttattttga
    17941 aaatggtcag ggacagtttg gtctgattta tgatgtggtc aaaggggatt gttaccacgg
    18001 tggtggggaa tttccagttt gtctaaatga taggccccta gctcctttta aaactaaacc
    18061 acttggagat tttctcattg cagggaatag tggtatgctg gaaaccaatg aatgggggct
    18121 ggctgatttg agccgagcgg ttcttggtgt tcgtgtctat ggaagtgcgg ccattagttt
    18181 tgccaagatt ttgtcagggc gtttgttgac ctatctcact tatctgcaac catgggatta
    18241 tgccgcagct agtattttag gggaaagtct gggctatcgg gttgtgaccc tttttggtga
    18301 agctcctgat tttcaaacca gacagccggt catgatggtg cctcttgaga tgcaggagga
    18361 aattcagtcc tatatttacg aaaggaaaag aacttaaatg caatttccag aaggatttgt
    18421 tgaaaaatat aaagagatac taggagatga ggcaagagat tttcttgcct cttttgagga
    18481 ggaagcggtt tcggcctttc gggtcaatcc cttaaaagaa gagcaacttt ccttttctga
    18541 tgccattact caaacccctt ggggccacta tgggaaggtt tcagggaaat cgcctgagca
    18601 tgctacaggt ttagtttatt cgcaagaacc tgctgctcaa atggtggctc aagtagctca
    18661 acccagtcct ggtatgaagg tcttggactt ggctgctgca ccggggggca aatcaactca
    18721 actggcagcc tatctagcag gggaggggct acttgtttcc aatgaaattt caagcaaacg
    18781 ggctaagatt ttggttgaaa acatggagcg ttttggagcg acaaatgtcg tggtgaccaa
    18841 tgaatctgcc gaccgcttgg tcaaggtctt taaaggctat tttgacttaa tcgttcttga
    18901 tgccccttgc tctggtgaag gaatgtttcg taagcaacct gatgctatgg actattggag
    18961 cttagattat ccgagtcaat gtgctagctt gcaaagagaa attctggagg atgcagtgac
    19021 catgttagct gaaggtggtc gtctggttta ttcgacctgt acctgggcac ccgaggaaaa
    19081 cgaagagatt gtcaattggt tgctggaaga gtatgatttt gacttgttgc cagtagagca
    19141 tatcaatgga atggtagctg gtattgacct gccagaaacg gctcggatgt accctcatca
    19201 gtttaaggga gagggtcagt ttgttgccca tctacagttt aagggtaaca atccagctcc
    19261 taaatttaag gcaagtaaga gtaacctcag ccgtgaacaa gttgccttgt ggcaggaatt
    19321 tgcccaaaac catttgaagg tcaatctacc gggtatcttg cagacttttg gagaccagct
    19381 ttatctcttg ccagaacttt tgccagattt agggaaactc aagattgctc gcaatggact
    19441 gcatctgggc acttttaaga agaaacgctt tgagcccagt ttcgctcttg gtttagcctt
    19501 gaaaccgagt caggtcgaac agtcggttga aattggccaa gaagcgtttg tcaagtatgc
    19561 ggctggagaa atcgttcagc tggcagaaag tctgccaaat ggttggtacc aagttttggt
    19621 taaaggcaat ggcttgggct ttgcaaaggt tactggaaat gttttgaaaa attattttcc
    19681 aaaaggcctc agattcaaat gaaaatgcta gtcaaatatc ttattctatg ttatagtgaa
    19741 agtatggatt tttttagtag gatgattctt gatatcttga ttgccattac atgtagtttt
    19801 actactcaat gaaaatcaaa gagcaaacta ggaagctagc catagatagc tcaaaacact
    19861 ggtttgaaga gattttcaaa gagtacaaga agtttagtta ttagcgttct taccgcttgt
    19921 aaactagatt tctcataaaa tagaatcttt tccttttagt tgtaaactag tctgggagag
    19981 tagagaggtt tgagatacct ttctagcttt tggattatca tctaaggaga gtaatttccc
    20041 ttgcattaaa aaggggaaaa agagacacga aatgactata atgggtgaca atgggggaag
    20101 ggatagacaa gagattttat ccacatatga aaaaaggagg ttaggaaaga gtaatatagc
    20161 ctatattata taaataatca attgcgcaga aatttggtaa gaattcatgc gtcaactcat
    20221 aaagaactac ttaaaaaatt cacagtattc ataattattt tcgaggagaa aaacagtgaa
    20281 aaaaagaaaa aagcttgctc tgtctcttat cgctttttgg ctgacggctt gtttagtagg
    20341 ctgtgctagc tggattgatc gtggagaatc cataacggct gttggctcaa ctgccttgca
    20401 acccttggtt gaagtagcgg cagatgaatt tggcacgatc catgttggaa aaacggtcaa
    20461 tgtccaaggg ggaggttctg gtacaggctt gtcccaggtt cagtctgggg cagttgatat
    20521 aggaaactca gatgtatttg ctgaggaaaa agacggaatt gatgcttctg ctcttgttga
    20581 ccacaaggtc gcggtagctg gcttggctct gattgtcaat aaggaggttg atgttgataa
    20641 cctaacgaca gagcaacttc gtcaaatctt cataggtgag gtaaccaatt ggaaagaggt
    20701 tggtggtaag gacttaccca tctctgttat caatcgggca gccggctctg gttctcgtgc
    20761 tacctttgat actgtcatta tggaaggtca gtctgccatg caaagtcagg agcaggattc
    20821 aaatggagcg gtaaaatcaa tcgtatcaaa aagtccagga gctatctctt atttatctct
    20881 tacctatata gatgattcgg tcaaaagcat gaagttgaat ggctatgact taagtccaga
    20941 aaatataagt agcaataatt ggcccttgtg gtcttatgag catatgtata cattggggca
    21001 gcccaatgag ttggctgcag aatttctcaa ttttgttctc tcggatgaga cccaagaagg
    21061 gattgtcaaa ggattgaagt atattccgat taaggaaatg aaggttgaaa aagatgctgc
    21121 cggaactgtg acagtgttgg aagggagaca ataatgaatc aagaagaatt agctaagaaa
    21181 atgttgcttc catcaaagaa ttctcgtctg gagaaattag gaaaaggttt gacctttgcc
    21241 tgtctttctt tgatagtcat ccttgtggcc atgattttgg ttttcgtagc gcaaaaaggc
    21301 ttgtcgacct tctttgtcaa tggtgtgaat atctttgact ttcttttggg aggaacttgg
    21361 aatccttcta gtaaagaatt tggtgccctt cctatgattt tgggttcctt tatcgttacc
    21421 attctctcag cccttatcgc aacacccttt gctattggtg cagcagtttt tatgaccgaa
    21481 gtatcaccaa aaggggcgaa gattttgcaa ccagctattg aactcctggt tgggattcct
    21541 tcagtagtgt acggatttat tggcttgcaa gtcgtcgttc cctttgttcg cagtgtcttt
    21601 ggtgggactg gttttgggat tttgtcaggg atttccgtcc tctttgtcat gattttgccg
    21661 accgtaacct ttatgacaac ggatagcttg cgtgcggttc ctcgttatta tcgtgaggcc
    21721 agtttcgcta tgggagccac tcgctggcag actatctggc gtgtgatctt gaaggcggcc
    21781 cgttctggta ttttcactgc agtggtcttt gggatggcgc gtgcctttgg tgaggcttta
    21841 gctatccaga tggttgtcgg aaactcagct gttatcccaa cttccttgac cacaccagct
    21901 gcaactttaa cttctatatt aactatggga attgggaaca ctgtcatggg aactgtaaat
    21961 aataatgttc tctggtcact ggccttggta ctgctcttga tgagtttagt ttttaacagt
    22021 gtgattaaat tgattacgaa agaaagagga aagaaaaatt atgcgcgcta agaaattaga
    22081 taaacttgca acagctgtcc tctatacgat tgctagcatc attgtgacaa tcttggcttc
    22141 cttaattctc tatatcttgg ttcggggctt gccccatatc tcttggtctt tcttgactgg
    22201 aaggtcttct gcttttcaag caggtggtgg gattggcatt cagctttaca attccttttt
    22261 cctattggtc attaccttga ttatttctgt acctctttct atgggagctg ggatttactt
    22321 ggctgaatat gctaaaaaag gtcctgttac caactttgtg cggacttgta ttgaaatctt
    22381 gtcctcttta ccatcagtgg tggtgggtct ctttggttac ttgatctttg tagtccagtt
    22441 tgagtatggg ttttcaatca tttcaggtgc cttggccttg acagtcttta acttgcctca
    22501 gatgacgcgt aatgtagagg atagtttgaa acacgttcac catacccaac gtgaggctgg
    22561 tctggctctt gggatttctc gctgggagac agtggttcat gttgttattc cagaagcgct
    22621 tccaggtatt gtaacgggtg tcgtcttggc atctggtcgt atctttggcg aagctgcagc
    22681 tctgatctat acagcagggc aatcggcgcc agctcttgac tggtctaact ggaatatcct
    22741 cagtgtgact agccccatct ctatcttccg tcaagcagaa accttggctg tccatatctg
    22801 gaaagtcaat agtgaaggca ctattccaga tggaaccatt gtatcagcag gttctgccgc
    22861 tgtgctcctg atctttatcc tgatttttaa ctttggagct cgtaagttcg gaatctatct
    22921 acacaagaaa ttaaccgctg cctaaaggag aagccatgtc aacatataac tgggatgaga
    22981 agcatatcct tacctttcct gaagaaaaag tagccctttc tactaaggat gtccatgttt
    23041 actatggtaa aaatgaatcc attaagggga ttgatatgca atttgaaaga aataaaatta
    23101 cagctttgat tggtccgtcg ggatcgggga aatctaccta cttacgcagt ctcaatcgca
    23161 tgaatgatac cattgatatt gctaaagtaa ctgggcagat tctctatcgt ggaattgatg
    23221 tcaaccgtcc agaaatcaac gtttatgaaa tgcgtaaaca cattggaatg gtttttcaac
    23281 gccccaatcc atttgctaag tcaatttacc gtaatattac ctttgcgcat gaacgtgctg
    23341 gagttaagga taagcaagtc ctagatgaaa tcgtagaaac ctcccttcgt caggctgccc
    23401 tctgggatca ggttaaagac gatctccaca agtcagcctt gaccttatca ggtggtcagc
    23461 aacaacgtct ctgtatcgct cgtgccatct ctgttaagcc agatatcctc ttaatggatg
    23521 agccagcctc agccttggat ccgattgcga ccatgcaact agaagagacc atgtttgagc
    23581 tcaagaaaaa ctttaccatc atcattgtaa cgcataatat gcagcaggct gctcgtgcaa
    23641 gtgactatac aggcttcttt tacttgggtg atttgattga gtatgacaag actgcaacta
    23701 ttttccaaaa tgccaagcta cagtccacca atgactatgt atctggtcac tttggttaga
    23761 aaggaaaccg tatgacagat gcgattttac aggtatcaga cctgtccgtt tattataata
    23821 aaaagaaggc tttgaatagt gtttccctat ctttccaacc taaggaaatt acagccttga
    23881 ttggtccatc tggatcaggg aagtcaaccc tcctcaagtc tctcaaccgc atgggagatc
    23941 tcaatccaga ggtgaccaca actggatccg tggtgtacaa tggtcacaac atctacagtc
    24001 cgcgtacaga tacggttgaa ttacgtaagg aaatcggaat ggttttccaa caacctaatc
    24061 ctttccctat gactatctat gagaatgttg tctacgggct tcgtatcaat ggaattaagg
    24121 ataagcaggt tctggatgaa gccgtagaaa aagccttgca aggtgcctct atctgggatg
    24181 aggtcaagga tcgtctatat gattcagcta ttggattgtc aggtggtcaa cagcagcgtg
    24241 tctgcgtggc ccgtgtcttg gcaactagtc ctaaaatcat cctcttggat gagccaactt
    24301 cggctttgga tccgatttca gctggtaaaa ttgaggaaac cttgtatggt ctaaaagaca
    24361 agtacaccat gcttctggta acccgttcca tgcagcaagc ttcacgtatc tctgataaga
    24421 caggattttt cctagatgga gatttgattg aatttaatga taccaagcag atgttcctta
    24481 atccccaaca caaggaaacg gaagactata ttacaggaaa atttggataa ggagatgaaa
    24541 gatgttacga tctcaatttg aagaagattt agagaaatta cataaccagt tctacgctat
    24601 gggacaagaa gtgctctcac aaatcaatcg tacggtacgt gcttttgtca cgcatgaccg
    24661 tgacctggca aaagaggtca tcgaagatga tgcagaagta aatgaatacg aagtgaaact
    24721 ggaaaagaaa tcatttgaaa tgatcgcact ccaacaacca gtctctcaag atttgcgtac
    24781 agtcttgact gtccttaagg ctgtatcaga tgtggagcgt atgggggatc acgctgtagc
    24841 cattgctcag gcaaccatcc gtatgaaggg ggaagagcgc attccagctg tagaggaaga
    24901 aattaaaaaa atgggacgtg aagttaaaag cgttgttgaa gcagcacttg atctttatct
    24961 taatggttct gttgacgacg cataccgggt ggcctccatg gatgagcaaa ttaaccacta
    25021 ttttgaaact atccgtgacc ttgcgactga agagattaag aaaaatccag aagccattgt
    25081 tacgggtcgt gattatttcc aagttatttc ctacttggag cgtatcggtg actatgctaa
    25141 aaatatctgt gaatgggttg tctactttga aacaggtaag attgtcgaac tataaaatag
    25201 tttaattctg tataaaaagg agcttgaaat caactgatgg ctcctttttt tgaacgaaaa
    25261 aatatttttg agataaaaat ttaaaaaaca cttgacaagt aacttggata tcgttataat
    25321 tatacaaaat taacagagag gttgtttatt tatgaaatca aaaaaatgga tatttgtttt
    25381 atgtaatttt cttgcaagtt tcttcttagt agcttgccag tcaggttcta atggttctca
    25441 gtcagctgtt gaggccatta agcaaaaggg gaaattagtt gtggcaacca gtcctgacta
    25501 tgcacccttt gaatttcaat cattggttga tggaaagaac caggtagtcg gtgcagacat
    25561 cgacatggct caggctatcg ctgatgaact tggggttaag ttggaaatct caagcatgag
    25621 ttttgacaat gttttgacca gtcttcaaac tggtaaggct gacctagcag ttgcaggaat
    25681 tagtgctact gacgagagaa aagaagtctt tgatttttca atcccatact atgaaaacaa
    25741 gattagtttc ttggttcgta aggctgatgt ggaaaaatac aaggatttaa ctagcctaga
    25801 aagtgctaat attgcagccc aaaaagggac tgttccagaa tcaatggtca aggaacaatt
    25861 gccaaaagct caattaactt ccctaactaa tatgggtgaa gcagtcaatg aattgcaggc
    25921 tggaaaagta gatgctgttc atatggatga gcctgttgca cttagttatg ctgctaaaaa
    25981 cgctggctta gctgtcgcaa ctgtcagctt gaagatgaag gacggcgacg ccaatgccgt
    26041 tgctcttaga aaaaatagtg atgatttgaa agaagtggta gacaaggtca tccaaaaact
    26101 taaggatgaa ggaatttacc aaagctatct tgaaaaagca gcaagtctaa ctgaagttga
    26161 agaataagaa aaagcagagt tggatgtcat tccaattctg cttttaaata gtttaaaaca
    26221 ttttccagat tgtctaggga caccttggca aattgataag gacaggtact taaataggcc
    26281 tcttcaaaga agtccaagcc cggttgactg tgtttgagac tggcgatttt aggatactgt
    26341 ctcaggtcta gcaataaacc atcgtgtagg ggataatgag gaaggggaca aggtgtttta
    26401 gttatccttt cctcgatttg tttttggtaa gattcatgat tggtcaagcg agccaaacga
    26461 tttttttcaa acaattgact gtcaatatag agtgacaggg ccttttgcat aatgaggttg
    26521 ctgtcgtagt ctaggatatt tttgtaggcc acaaatgctt ctttgatagc atttggaaga
    26581 atgagtgctg taatacgaag ggcaggaaaa aggctggtcg agaaggactt gatataaatg
    26641 acatgctcct ctgtatcaag atagtggaag gtttggccct tcttggagtc caaatcaccc
    26701 agataatcgt cttctacgat atagacatca tacttggcag ctaagttaag aatggatcgt
    26761 ttgtcttgct cagaatagga atgtcccaga ggatagtgaa atcggggaat ggtgtagaaa
    26821 aacttaattt ttcctgtttt gaagtggcct tccagctcct ccaagtcaat cccatcaatg
    26881 cctcgttcaa tcgtttgata gtccagcccc tgtgcaatca agaggcgatt catccgatgg
    26941 taggttggct gttccaccaa gatttccttg gcttgtctag gaaaggatat ttgagagagg
    27001 ataaataagg cttgttgggt tccagaagtc agtactagtt ggttagcctt gcagtagaga
    27061 gcttgctcaa agaggagttt gtgaatggac tgtcttaggt cttctaatcc ttcttgattg
    27121 tcatagtagt tgaagaggta gttttctcgt ccaatcaagg tttcattgac acagagtcgg
    27181 aaatcgtcat aggcactggc atgttcgtcg gtaacctcga tttctaggtc ttgatgttgc
    27241 ccttgttcta atacatagta gccactctga ggcttggcat agaggtattg ttcgtgccgt
    27301 aattccagca gggctcgttg aatggtgtcc ttgctgcagt gaaagtcaag gctcagttga
    27361 cggatagaag gcaggcgact acccgtcgga aatcgtccag actcgatacc atttttcaga
    27421 taggaaacga cctctttgta cttgctttgt ttcttcattc cagacctccc ttgattttgt
    27481 tacattgtac cctttttttt ccttcttggc aatgtctaga gcgtttctct cgttcacatc
    27541 taggggcaaa tgtggtatac ttaggagtaa gatttataga ataacagaca agaaagtgaa
    27601 tggataagaa cgtgcaggaa ccagtgaaat tatttcaata caatactctt ggagccttga
    27661 tggctggcct ttatggtggt actatgacag taggagaatt gctagaacat ggtgaccttg
    27721 gtttgggaac cttggattcc attgatgggg aattgattgt cttggatggc aaggcttatc
    27781 aggccaaagg gtcaggagac cagccagaaa ttgtggaagt gtcaccagat gcccttattc
    27841 cttacgctgc agtggtaccg catcaggcag aggtcatttt ccgccagcgc tttgagatga
    27901 cagacaagga attgaaagaa cgaattgagt cttattatga tggggaaaat cttttccgct
    27961 ctatcaagat tcggggggaa ttttcacata tgcatgtgcg tatgattcct aagtcaacac
    28021 ccgataccaa gtttgctgat gtcgcaaccc atcaaccgga atatagtcgt gacaatgttg
    28081 cggggactat tgttggtttc tggacgcctg agattttcca tggggtcagt gtggcaggct
    28141 accatctgca cttcatatca gatgatttga ccttcggtgg acatgtcatg gattttgtca
    28201 tcaaggaagg cattatcgag gtgggagcag ttgaccagtt ggaccaacgt ttcccagtcc
    28261 aagaccgtca atacttgttt gctaagttca atgttgacga gatgaaaaaa gatattgaaa
    28321 aggcagaata ggagaagaaa atgaccattc atatcattat taccatgttg ctgttgctag
    28381 cttttctgat aggaagcatt tggtttgcca aaaagaaata ccagattaat ctagctgtct
    28441 tgggcttggg tgcagtcgct ttctttgtct cttcgcagat tttggaaaaa ctggtgcata
    28501 tcttgatttt gcatcctcaa aaagacggta gtattgccct cttgcaagat catccgctta
    28561 tctatattat ctatggccta gccatggcag cattttttga ggaaacggct cgtcttgttt
    28621 tcttcaaatg gttggagaaa aagagaagtt tggaaaaggc agatgccttg gcctatggcc
    28681 tagggcatgg tggcttggag ttgatttttc taggccttac tagtttactc aatctttaca
    28741 tcgttctttc ggcagttcaa actcagaatc cacaggttat gcaattactg tctgaaaata
    28801 tgttaaaaac cattcagtca ctttcggtct ggcagattta tttgcttggt tttgagcgaa
    28861 tcttggcgct tggtttccaa ttacttttga cagtttgggt ttaccaagct gttcgccaga
    28921 agaaatggat ttatctccta gcagcctatg gcttgcatgc cttctttgat ctggcaccat
    28981 ctcttttcca agtaggctgg ttgacaaatc cagtcttggt tgaagtgatt ctagcactgg
    29041 agctcgttct ggtcgcctat ggaaccaagg aaatcttttg taaaaaatca taaaaagggg
    29101 gaaacctctt tttcttatgc aaaatccaaa caaggtattt ttatggtcgt caaatgtctc
    29161 taaaaatggt ataatggaat gaattttgta aaaggaagag tgacatgtct gtaagagaaa
    29221 aaatgcttga aatcttagaa ggaattgata tccgttttca ggaacccttg catagctata
    29281 gttatacaaa agtaggtgga gaggctgatt atttggtctt tccacgaaat cgttttgagt
    29341 tggctcgcct tgtgaaattt gccaaccaag aaaatatccc ttggatggtt cttggcaatg
    29401 ccagcaatat catcgttcgt gatggtggga ttcgtggatt tgtcatcttg tgtgacaagc
    29461 tcaataacgt ttctgttgat ggctatacca ttgaagcaga agctggggct aacttgattg
    29521 aaacaactcg cattgccctc cgtcatagtt taactggctt tgagtttgct tgtggtattc
    29581 caggaagcgt tggcggtgct gtctttatga atgcgggtgc ctatggtggc gagattgctc
    29641 acatcttgca gtcttgtaag gtcttgacca aggatggaga aatcgaaacc ctgtctgcta
    29701 aagacttggc ttttggttac cgccattcag ctattcagga gtctggtgca gttgtcttgt
    29761 cagttaaatt tgccctagct ccaggaaccc atcaggttat caagcaggaa atggaccgct
    29821 tgacgcacct acgtgaactc aagcaacctt tggaataccc atcttgtggc tcggtcttta
    29881 agcgtccagt cgggcatttt gcaggtcagt taatttcaga agctggcttg aaaggctatc
    29941 gtatcggtgg cgtagaagtg tcagaaaagc atgcaggatt tatgatcaat gtcgcagatg
    30001 gaacggccaa agactacgag gacttgatcc aatcggttat cgaaaaagtc aaggaacact
    30061 caggtattac gcttgaaaga gaagtccgga tcttgggtga aagcaagtag ggagctgtat
    30121 ttgaaaagct gctgctatgt catggaaagg ggggtgaaag cctatcggta gcgaagatgt
    30181 atgcaggtgg ttttactccc tgcaagaggt agtggcgacc tgacagagcc ccgatctgtt
    30241 aatctatgaa aaagaaggaa tttataccaa ttgaaaaaac caattatcga attcaaaaac
    30301 gtctctaaag tttttgaaga cagcaacacc aaggttctca aagacatcaa ctttgagttg
    30361 gaagaaggga aattctacac ccttctaggt gcatctggtt cggggaaatc aactatccta
    30421 aacattattg caggtttact ggatgcgacg acaggagata tcatgctaga cggtgttcgt
    30481 atcaatgata ttccaaccaa caagcgtgat gtccatacgg tcttccaatc ctatgccttg
    30541 tttccgcata tgaatgtgtt tgaaaatgtt gcctttccac ttcgcttgcg caagattgac
    30601 aagaaagaaa tcgagcaacg cgtagcggaa gttctcaaga tggttcagtt ggaaggttat
    30661 gaaaaacgtt ccatccgcaa actttctgga ggacaacgtc agcgtgtggc tatcgcccgt
    30721 gctatcatca accaaccccg tgtggttttg ttggatgagc ccttgtcagc gctggacttg
    30781 aaattaagaa cagacatgca gtacgaactg cgtgaactgc aacaacgatt gggcattacc
    30841 tttgtctttg tcactcacga tcaggaagaa gctcttgcca tgagtgactg gatttttgtt
    30901 atgaatgatg gcgagattgt ccagtctgga actcctgtgg atatttatga tgaaccaatc
    30961 aaccactttg ttgccacctt tatcggggag tcaaatatct tgccaggtac catgattgag
    31021 gactacttgg ttgagtttaa cggcaaacgc ttcgaagcgg tcgatggggg gatgaagcca
    31081 aatgaacctg ttgaggttgt tattcgtcca gaggacttgc gcattaccct tcctgaagaa
    31141 ggcaagctcc aagttaaggt cgatacccag cttttccgtg gagttcatta tgaaattatc
    31201 gcctatgacg aacttggaaa tgaatggatg atccactcaa cccgtaaggc catcgtgggt
    31261 gaggaaatcg gtctggactt tgaaccagaa gacatccaca tcatgcgtct caatgaaacc
    31321 gaagaagagt tcgatgctcg tattgaggag tacgtagaaa tcgaagagca agaagcaggt
    31381 ttgatcaatg caatcgagga gaaaagagat gaagaaaaca agctctaaac tctttgtagt
    31441 gccctacatg ctttggattg cgctctttgt attggcaccc ttggtcttga ttttcggtca
    31501 atcctttttc aacatcgaag gccagttcag tttagaaaat tacaaatctt actttgcgtc
    31561 acaaaacttg acctatctta aaatgagttt caactcagtg ctttatgcag gcattgtgac
    31621 ctttgtgaca ctgcttatca gctatccgac ggccctcttt ttgacccgtc tcaaacaccg
    31681 tcaactctgg attatgctga ttattctgcc aacttggatc aaccttcttc ttaaggctta
    31741 tgcctttatc gggatttttg gtcagaatgg ctctattaac caattcttgg aatttatcgg
    31801 aattggttca cagcagttgc tatttactga tttctcattt atctttgtcg caagctacat
    31861 cgagctcccc tttatgattt tgccgatttt caatgtcttg gacgatatgg ataataatct
    31921 catcaatgct agttacgacc ttggtgcaac taagtgggag actttccgtc atgtcatctt
    31981 ccctctatct atgaacggtg tgcgaagtgg ggttcagtcg gtctttatcc caagtttgag
    32041 tctcttcatg ctgacccgtt tgattggtgg gaaccgcgtt atcaccttgg ggacggctat
    32101 cgagcagaat tttctaacca atgacaacta tggtatgggt tcagccattg gtgtgattct
    32161 catcctgacc atgttcatca ccatgtgggt gaccaaggaa aggagagaac gatgaaaaaa
    32221 tttgccaacc tttatctggg actggtcttt cttgtcctct acctgccgat tttttacttg
    32281 attggctacg cctttaatgc aggaaacgac atgaacagct ttacaggctt tagcttgagc
    32341 cattttaaaa ccatgtttgg tgatggttgc cttatgttaa ttgtgactca gacctttttc
    32401 ttggccttcc tatcagcctt gatagcgacc attatcggga cttttggtgc catttacatc
    32461 taccagtctc gtaagaaata ccaagaagcc tttctatcac tcaataatat cctcatggtt
    32521 gcgcctgacg ttatgattgg tgctagcttc ttgatactct ttacccaact caagttttca
    32581 cttggctttt tgaccgttct atctagtcac gtggccttct ccattcctat cgtggtcttg
    32641 atggtcttgc ctcgactcaa ggaaatgaat ggcgacatga ttcatgcggc ctatgacttg
    32701 ggagctagtc aatttcagat gtttaaggaa atcatgcttc cttacctgac tccgtctatc
    32761 attgcaggtt acttcatggc cttcacctat tcgctggatg attttgccgt gaccttcttt
    32821 gtaacgggga atggcttttc aaccctgtca gtcgagattt actctcgtgc acggaaaggg
    32881 atttccttag aaatcaatgc cctgtctgct ctagtctttc tctttagtat tatcctagtt
    32941 gtaggttatt actttatctc tcgtgagaag gaggagcaag catgaaaaaa atctattcat
    33001 ttttagcagg aattgcagcg attatccttg tcttgtgggg aattgcgact catttagata
    33061 gtaaaatcaa tagtcgagat agtcaaaaat tggttatcta taactgggga gactatatcg
    33121 atccagaact cttggagcaa tttacagaag aaacaggcat ccaagttcag tacgagactt
    33181 ttgactccaa cgaagccatg tacactaaga taaagcaggg tggaacgacc tacgatattg
    33241 ccattccaag tgaatacatg attaacaaga tgaaggacga agacctcttg gttccgcttg
    33301 attattcaaa aattgaagga atcgaaaata tcggaccaga gtttctcaac cagtcctttg
    33361 acctaggtaa taaattctcc atcccttact tctggggaac cttaggaatt gtctacaacg
    33421 aaaccatggt agatgaagcg cctgagcatt gggatgacct ttggaagtcg gagtataaga
    33481 attctatcat gctctttgat ggggcgcgtg aggtgctggg actaggactc aattccctcg
    33541 gctacagcct caactccaag gatccgcagc agttggaaga gacagtggat aaactctaca
    33601 aactgactcc aaatatcaag gctatcgttg cggacgagat gaagggctac atgattcaga
    33661 acaatgctgc tatcggtgtg accttctctg gtgaagccag ccaaatgcta gaaaaaaacg
    33721 aaaatctacg ctatgtggta ccgactgaag ccagcaacct ttggtttgac aatatggtca
    33781 ttcccaaaac agtcaaaaac caagacgcag cctatgcctt tatcaacttt atgttgaaac
    33841 ccgaaaatgc tctaaaaaat gcggagtatg ttggctattc aacaccaaac ctaccagcta
    33901 aggaactgtt accagaggaa acaaaagaag ataaggcctt ctatcccgat gttgaaacca
    33961 tgaaacacct agaaatttat gagaaatttg accataaatg gacagggaaa tatagcgacc
    34021 tcttcctaca gtttaaaatg tatcggaagt aggagataaa cgtaagaaga cgaatcagtc
    34081 aagctggttc gtttttaagg aatagaagtc agtttttaga gccctatctt tttctattct
    34141 agttgtttat ttgtttattt gcttatttgc ttgcctagta gtatactaag cctatactct
    34201 tcaaaccacg tcagcgttgc cttgccgtac tcaagtacag cttgaggcta gcttcctagt
    34261 ttgctttttg atttttattg agtattaacc ttacagaaag cggcaacaaa taatgaaaat
    34321 gtctactttt ttcaaaaaaa gtttctggcc aacctttatg attgttaacc aaactgctat
    34381 actattccat ttaaaggatg gcttggatcg acaataccta acaactgagt ccatatattg
    34441 ggtgataggc acctttatat ttggaaatat tctagtagct gtgtttagta atatgaaaat
    34501 ttgggataaa aagaaaaatg gcagtaagaa aaaatatatc ctgaaaaagt aatcagagat
    34561 gtagggaatt ggtctaattc aacggagatc atttccagtt gacgcaagct taaaaaaacg
    34621 ttataataag aaagttgaga attgattttc acttgtctta gtccagagaa atggcggtgc
    34681 tgcgagccat ctaagctagg aagtcatgct actcaatcat acaattgcat aagaacaaga
    34741 gaatgacaag ttcattgaat gaaggtggta ccgcggtttt tcgcccttcg tgatatgagc
    34801 ttgtcttttg atttttggag gtgttgatga aaacatttct tgtcaaacaa aagtttcgtc
    34861 ttggaggcga acgcttcgct atcaaggatg acaggggaga aattgcctat caggtggagg
    34921 gatcattttt taagattcct aaaaccttta ccatctatga tgcggctggt gaacaagtca
    34981 gtgagattag taaagaaatc ttgaccttac ttcctcgttt tgagattcag cttcgggatg
    35041 gctcgagttt tgtcattcgt aagaagttga ccttctggcg agataagtat gagtttgata
    35101 atctaggtct tcgtatcgag ggcaatatct gggatttgaa tttcaaattg ctggatgatc
    35161 gcgatcagct gattgcggaa attaagaagg aactcttcca tctgacctct acctataccg
    35221 taacggttct tgaggacgct tatgcagacc tagtcatttc cctctgtgtc gcgattgact
    35281 atgtggaaat gctggaaagc caatcacatt aaacaagtaa ataaggagac aatatgaaac
    35341 aactatctag tgcacaagta cgccaaatgt ggcttgattt ctgggcgacc aaagctcact
    35401 cagtagaacc atcagtaagt ttggttcctg taaatgaccc aactcttttg tggatcaact
    35461 ctggggtagc aacccttaag aaatactttg acgggaccat tatccctgaa aatccacgta
    35521 ttaccaatgc ccaaaaggct atccgtacca acgacatcga aaacgtaggg aagactgcgc
    35581 gtcaccatac catgtttgaa atgctgggga acttctctat cggggattac ttccgtgacg
    35641 aagctatcac ttgggcttat gagcttttga caagccctga atggtttgat ttccctgctg
    35701 aaaaacttta catgacctac tatccagacg ataaagattc ttacaaccgc tggattgaag
    35761 tgggagtgga tccaagtcac ttgattccaa ttgaggacaa cttctgggaa atcggtgcgg
    35821 gaccttctgg accagataca gaaatcttct ttgaccgtgg ggaagccttt gacccagaaa
    35881 atatcggtct tcgcctgctt gcagaagata ttgaaaacga ccgttatatt gaaatctgga
    35941 acatcgtttt gtcacaattt aacgcagacc ctgctgttcc tcgtagcgaa tacaaggaat
    36001 tgccacataa gaacattgat acgggcgctg gtttggagcg tttggtggct gttatccaag
    36061 gggctaagac caactttgaa acggacctct tcatgccgat tatccgtgaa gtcgagaaat
    36121 tgtctggtaa ggtttatgac caagatggcg acaacatgag cttcaaggtt atcgcagacc
    36181 acattcgttc tctttcattt gctatcggtg atggtgccct tccaggaaat gaaggtcgtg
    36241 gttatgttct tcgtcgtctt ctccgtcgtg cttctatgca tggtcaaaaa ttgggtatca
    36301 acgaaccttt cctttacaaa ttggttccaa ctgttggaaa aatcatggaa agctactacc
    36361 cagaagtgct tgagaaacgt gactttattg agaaaatcgt taagagcgaa gaagaatcat
    36421 ttgcccgtac ccttcactca ggtcaacact ttgcccaagg cattgtagca gacttgaaag
    36481 aaaaaggtca atctgttatc gctggttcag atgtcttcaa actctatgat acttatgggt
    36541 tcccagttga attgactgaa gaaatcgctg aagaagctgg tatgactgta gaccgtgaag
    36601 gttttgaagc agccatgaaa gaacagcaag aacgcgcgcg tgcctcagct gtcaagggtg
    36661 gctcaatggg tatgcaaaat gaaactcttc aaaacatcac tgtagaaagt gtcttcaact
    36721 acaatgctag ccaattgtct tctaaattgg tagctatcgt ggcggacaat gcagaagtag
    36781 aagctgtttc agaaggaact gcctctctta tctttgcgga aacgtcattt tatgctgaaa
    36841 tgggtggaca ggtagctgac tacggacaaa tcttggatga gtcaggtaag gttgtggcta
    36901 ctgtgaccaa tgttcagaaa gccccaaatg gtcaagccct tcatacagtt gaagtccttg
    36961 caccgcttgc cttgaaccaa gaatatacct tggcaattga tagcaatcgc cgtcaccgtg
    37021 tcatgaaaaa ccacactgcg actcatttgc ttcacgctgc ccttcacaat atccttggaa
    37081 accacgcaac acaggcagga tctcttaacg aagttgaatt ccttcgcttt gactttaccc
    37141 acttccaagc tgtaactgct gaagaattgc gtgcgattga acagcaagtc aacgaaaaaa
    37201 tctgggaagc tcttgaagtt aagacagttg aaacggatat tgacactgct aaagaaatgg
    37261 gagctatggc cctctttggt gagaaatacg gcaaggaagt tcgtgtcgtg actatcggtg
    37321 actactctat tgaactttgt ggtggtactc atgttggcaa cacttctgag attggtctct
    37381 tcaaaattgt caaagaagaa ggaatcggtt caggaactcg ccgtatcttg gcagtgactg
    37441 gtaaggaagc ctttgaagcc tatcgtgaac aagaggatgc tcttaaagct gtcgcagcaa
    37501 tcttgaaagc acctcaagtc aaggaagtac ctcacaaggt agaaggactt caagaacaac
    37561 ttcgtcaact tcaaaaagaa aatgctgagt tgaaagaaaa agccgcagct gcagccgcag
    37621 gcgatatctt caaagatgct aaggaagtca acggtcatcg ttacattgct agtcaagtgt
    37681 ctgtatccga tgccggtgcc cttcgtactt ttgcagataa ctggaaacaa aaagactact
    37741 ctgatcttct tgtcctagtt gccgctatcg gtgacaaagt caatgtcctt gtagcaagca
    37801 agacaaaaga ccttcatgca ggaaaccttg tcaaagaatt agcaccaatc atcgatggac
    37861 gtggtggtgg taaaccagac atggccatgg caggaggaag caaccaagct aagatccaag
    37921 aattgttgga tgcagtagca ggtaaattgt aatcaagtaa agatctatcc atttgggtag
    37981 gtctttttgt gaatacaaaa aagccaaatc cgcttggacc tagcttggtt attatgagct
    38041 attagatggt attgactgcc cagacactta cggatctagc tgagacaggg aattgtccat
    38101 aaccttcctc atcaattgta acttgacctt ggtggttacc aagtaaatct acaaaggttt
    38161 gattagtcca ttcttgaccg acaaacattg acttgctgtt ttcttggtca tttgagataa
    38221 ggactgcgat tggggattga ttttcagcac ctgaacgtac ccaaccgata cagttagcat
    38281 ggtcaaagta gtcattttgt tctccatagg ccaagtcttt tcggatggct aggaggcggt
    38341 caaggatttc tttgaaatct tcttgagcat actggcctga aatcccatag tagtctccgt
    38401 aaaagacaca tggaaggccg tcttggcgta acaaaatgag ggcataggct gctggcttga
    38461 accattcttc aacggtagac tcaagggctt gtcctcgttg ggtatcgtgg ttgtcgacaa
    38521 aagtcacagc cttgtcaggc ttgagttcaa ccaggctatc tgtgaaaatg ccacgaaggt
    38581 catagtttgc gccagcttga ctggcttcaa agagattctg gtggagacga acatcgacaa
    38641 ggtcaaagtg ttcttccgtt ttttcgagat agtccagatt ggcttccttg tctgggttcc
    38701 aaaattcacc aaaaacatag aaatcgtcac cgtatttttc cttcatatcg cggatgaagt
    38761 tgcgcataaa gaaagagtca atatgcttaa cggcatccaa acggaaacca gctacaccag
    38821 tcgtttccat gaaccaatca gcccagtcat agatgttttg gatgacttca ggatgtttaa
    38881 agtctaggtc ggcatacatg aggtagtcgt agtttccgtt ttcgttatcg accaattcct
    38941 cgttagccca gcccttgttg tccccttgga tcagataaat cccagattta ctgcgtttgg
    39001 catcgtagtc tgtaccggtg aagtggtacc aatgccagtg gaagccatta taggtatctt
    39061 ggcgaccatc gaaggtaaaa ctagtccagc cattgatggt gaagggttct ccaagttcaa
    39121 ctgtacggtc tacaggatca acttcgataa cctgaaaggc ttccctgtta tcggcagcag
    39181 ccttgtggtt gagaactaca tcggccatag gttgaattcc ctgttcttta agggcttgaa
    39241 tggcttgaag atagtcttct ttgaaaccat acttggtgcg gacagtccct ttttggttga
    39301 actctcctaa gtcaaataag tcatagaccc catagccgac atctttttcg ttggttgcct
    39361 tgaaggctgg tggcatccag acgtgactga tccccagatg agctaggtgt ggagcatttt
    39421 cagccagacg cgtccagtgt tgaccgtcgt ggggcagata ccattcaaag tattgcatga
    39481 gtgtttgatt ttgcatgatg tttcctctta cttgtcaatc ttgttcttta ttctatcata
    39541 aagtatctct cggcgcaaac gtttgcgtaa gatagaagga aactatttta actacttaaa
    39601 ataaagtatt gatttttttg tatcaaagtg ctagtatagc gatgtataat acagataaag
    39661 ataggagttg tcccatgatt gaatttcaaa atgttagtaa gttgtatggt gataaagagg
    39721 cattgagcaa tctcaatttg cagattgaaa atggggagat tatgggcttg attggtcata
    39781 atggggctgg aaaatcgacc accataaaat ccctagtcag tatcatttca cccagcagtg
    39841 gtcgtatttt ggtagacggt caggagttat cggaaaatcg cttggcgatc aaacgaaaga
    39901 ttggctacgt agcagactcg cctgacttat ttttacgatt aacagccaat gaattttggg
    39961 aattacttgc ctcgtcctat gagctgagta gctctgactt ggaggctagt ctagctaggc
    40021 tattgagcgt ttttgatttt gctgaaaatc gctatcaggt tattgaaact ctttctcacg
    40081 gaatgcgtca gaaagtcttt gtcatcgggg cgctcttgtc tgatcccgat atttgggtct
    40141 tggatgaacc cttgactggt ttggatcccc aggctgcctt tgatttgaaa cagatgatga
    40201 aggaacatgc acaaaaaggg aagacagtct tgttttcaac tcatgtccta gaggtggcag
    40261 agcaagtctg tgatcggatt gccattttga aaaaggggca tttgatttat tgtggtagtg
    40321 tagaggactt gagaaaagac aacccagacc agtctttgga aagtatctac cttagccttg
    40381 ctggtagaaa agaggaggtt tcagatgcgt ctcaaggtca ttaaaaaatt agttgatatc
    40441 aatatcctct attcatctca agaagctaat ctggctaatc tacgaaagaa gcaggctaag
    40501 aatcctggga aaaaagtaaa tgtttccgct agagtcctaa gttcttacat tttttctagt
    40561 ctcttgatgc ttttcatgtt tataaatata gcctttcgtt ttccttttga ggaaatgcca
    40621 agttttttta gtacgatggt tgctatttta ctggtgctgg ccttttcaac ttctttcact
    40681 gcattttaca atgtctttta tgagagtaag gacctggcct cctataggcc ctatgccttt
    40741 aaagaatcag agattataat ggccaaaggt ctgtctgtcc tcttgccagc tctaactgga
    40801 attgtaccaa tcctagctta ttttctggtc ctctacatta ggctagcacc ttctctgtgg
    40861 ttgggtttgc ttttgatgct actgtccttg accttattat ttgtctctgt tactttagtg
    40921 acggtagtgg cagtacattt cttggctcag actagggtct tcagaaagta tcagtctatt
    40981 ttttcgaatg tgatgattgg gataggagtt ctcatacctt taatatttat cttctttctt
    41041 cagtcgactt ttggaagtat tgttgacaaa gttagagaca ttccatttct cctttatcct
    41101 cttcatatct tttacaaaat agcagtggag cctttttcga cagaagcctt agtgggtctg
    41161 ctcgcttgga taggactaac tctcttcctg ctttatctga ccaaaaagaa ggtccttcct
    41221 cgtttttatg acgtgatcct gcttaacagt gaggagaagg tcaaaaaaga acgtcgcagc
    41281 aaggatagga tttcaactac taaaaagggc tttttccgta tggttttacg ctaccacctc
    41341 accctcttgg gacaggggac tggcgtgatt acagtgcttt ttacaagtgc tttccttcct
    41401 tatctcatga tgatcagtct gatttccaaa atccgagatt ctcagatagt tccagacatt
    41461 catcctccat actggttacc cttgtttttt gtaggagtgt ttatagcagt tgtcaataac
    41521 aatatcacca gcctgcattc aattgccttg tccttggaga gggaaaatgt tgattttctt
    41581 aagagtttac cctttgactt tgctcgttat gtgaaagtga aattttggat tatatatgcc
    41641 gtccagtcct ttttaccaat tctgacttta cttggtcttt ctctatatct aggcttgccc
    41701 atcatttcga tgatttacct tattatggca tggatccttg ccagtgtcat cctttcttgc
    41761 caccattact ttaaggacgt taaaaatctg tcaaccaatt ggagtagcat tacggacctg
    41821 gtgaatcgtt caaatggcat agtcgccata gttttattgt ttatttatag tgcaatcctg
    41881 atggcccttg taattgggag catattcttg gttcagtctc tctccactat ccttgccatc
    41941 agcttgggag taggagctct tatcctcctg cttgctcttg ctatttttgg ctatcattat
    42001 tacctgtcac gcatattggc agaaatagaa aaaagatgag ctggttggtc gcttgcttga
    42061 taaattcaat tcgacaaacg cttttatagt ttgttagaat gtagttgtaa atgattaaga
    42121 ttggtaatca aaaatatagg gaggaatgtt ttgaat
//
LOCUS       DAVKLX010000013        39845 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKLX010000013 DAVKLX010000000
VERSION     DAVKLX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39845
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.013"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            564..2717
                     /locus_tag="VW325_000766"
     CDS             564..2717
                     /locus_tag="VW325_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9734204.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2730..4079
                     /locus_tag="VW325_000767"
     CDS             2730..4079
                     /locus_tag="VW325_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9734205.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4249..4956
                     /locus_tag="VW325_000768"
     CDS             4249..4956
                     /locus_tag="VW325_000768"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9734206.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            5013..8738
                     /locus_tag="VW325_000769"
     CDS             5013..8738
                     /locus_tag="VW325_000769"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9734207.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVVSDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8831..10273
                     /locus_tag="VW325_000770"
     CDS             8831..10273
                     /locus_tag="VW325_000770"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9734208.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     CDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10492..11514
                     /locus_tag="VW325_000771"
     CDS             10492..11514
                     /locus_tag="VW325_000771"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9734209.1"
                     /translation="MANKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIAGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11511..12056
                     /gene="purN"
                     /locus_tag="VW325_000772"
     CDS             11511..12056
                     /gene="purN"
                     /locus_tag="VW325_000772"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9734210.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12140..12649
                     /locus_tag="VW325_000773"
     CDS             12140..12649
                     /locus_tag="VW325_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9734211.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12674..14221
                     /gene="purH"
                     /locus_tag="VW325_000774"
     CDS             12674..14221
                     /gene="purH"
                     /locus_tag="VW325_000774"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9734212.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     KPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14343..15605
                     /gene="purD"
                     /locus_tag="VW325_000775"
     CDS             14343..15605
                     /gene="purD"
                     /locus_tag="VW325_000775"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9734213.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTAIYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQLILPRLTSDFAQNITDILDSKEPNITWTDKGVTLGVVVA
                     SKGYPLDYSKGVELPVKTDGDIITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            16008..16496
                     /gene="purE"
                     /locus_tag="VW325_000776"
     CDS             16008..16496
                     /gene="purE"
                     /locus_tag="VW325_000776"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9734214.1"
                     /translation="MKPVISIIMGSKSDWATMQKTTEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16483..17574
                     /gene="purK"
                     /locus_tag="VW325_000777"
     CDS             16483..17574
                     /gene="purK"
                     /locus_tag="VW325_000777"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9734215.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17584..17811
                     /locus_tag="VW325_000778"
                     /pseudo
     CDS             17584..17811
                     /locus_tag="VW325_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
     gene            17874..19172
                     /gene="purB"
                     /locus_tag="VW325_000779"
     CDS             17874..19172
                     /gene="purB"
                     /locus_tag="VW325_000779"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9734216.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19227..23165)
                     /gene="strH"
                     /locus_tag="VW325_000780"
     CDS             complement(19227..23165)
                     /gene="strH"
                     /locus_tag="VW325_000780"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9734217.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMDFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQEEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(23480..24196)
                     /locus_tag="VW325_000781"
     CDS             complement(23480..24196)
                     /locus_tag="VW325_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9734218.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            24544..26331
                     /locus_tag="VW325_000782"
     CDS             24544..26331
                     /locus_tag="VW325_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9734219.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRIPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLNNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26328..26804
                     /locus_tag="VW325_000783"
     CDS             26328..26804
                     /locus_tag="VW325_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9734220.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            26832..27737
                     /locus_tag="VW325_000784"
     CDS             26832..27737
                     /locus_tag="VW325_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9734221.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27724..28539
                     /locus_tag="VW325_000785"
     CDS             27724..28539
                     /locus_tag="VW325_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9734222.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            28546..28950
                     /locus_tag="VW325_000786"
     CDS             28546..28950
                     /locus_tag="VW325_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9734223.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            29249..30415
                     /locus_tag="VW325_000787"
     CDS             29249..30415
                     /locus_tag="VW325_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9734224.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            30531..31568
                     /locus_tag="VW325_000788"
     CDS             30531..31568
                     /locus_tag="VW325_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9734225.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            31776..32630
                     /locus_tag="VW325_000789"
     CDS             31776..32630
                     /locus_tag="VW325_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9734226.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            32767..33024
                     /locus_tag="VW325_000790"
     CDS             32767..33024
                     /locus_tag="VW325_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU9734227.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(33066..33428)
                     /locus_tag="VW325_000791"
     CDS             complement(33066..33428)
                     /locus_tag="VW325_000791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734228.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEEYKSLHNKTHITKVLMNCTPKVRQKKSNFWGSVHSTPDTMR
                     FFNFRDFLQLLLTHIKLF"
     gene            complement(33482..33778)
                     /locus_tag="VW325_000792"
     CDS             complement(33482..33778)
                     /locus_tag="VW325_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9734229.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(33768..33929)
                     /locus_tag="VW325_000793"
     CDS             complement(33768..33929)
                     /locus_tag="VW325_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU9734230.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            33999..34763
                     /locus_tag="VW325_000794"
     CDS             33999..34763
                     /locus_tag="VW325_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9734231.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(35003..35155)
                     /locus_tag="VW325_000795"
     CDS             complement(35003..35155)
                     /locus_tag="VW325_000795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734232.1"
                     /translation="MRTKASLRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            35373..36752
                     /locus_tag="VW325_000796"
     CDS             35373..36752
                     /locus_tag="VW325_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9734233.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            36766..37431
                     /locus_tag="VW325_000797"
     CDS             36766..37431
                     /locus_tag="VW325_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9734234.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            37545..37640
                     /locus_tag="VW325_000798"
                     /pseudo
     CDS             37545..37640
                     /locus_tag="VW325_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            37786..38324
                     /locus_tag="VW325_000799"
                     /pseudo
     CDS             37786..38324
                     /locus_tag="VW325_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            38391..38801
                     /locus_tag="VW325_000800"
     CDS             38391..38801
                     /locus_tag="VW325_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9734235.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            38917..>39845
                     /gene="pavB"
                     /locus_tag="VW325_000801"
     CDS             38917..>39845
                     /gene="pavB"
                     /locus_tag="VW325_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9734236.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASLAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNV"
BASE COUNT        10977 a         7746 c         9275 g        11847 t
ORIGIN      
        1 ttttagcatt ttactttgtt atttatttaa tccatcggat ataaagtttg cctatttcag
       61 cgtattgaca gtattactaa ttgggataac aaattatgcg agatttaaaa atttaagatt
      121 gacagtagtg ttttctgtac catttttatt taatgctata atgtctatta taaatagttt
      181 ttagaaatac taaaattttt ttctttactt aataatgata tgaaatcatt tcggttttgt
      241 taacttgtaa ttcttatatt ttctaggtag aacgtttgtt ctgcctattt ttttatccaa
      301 aaagtgcagt tgggagggag ataggctcat ttgggaagga agtccagttt ttgtttagtg
      361 attggggtaa gatagttgtt atcagatgag tttatactct tcgaaaatca aattcaaacc
      421 acgtcaacgt cgccttgccg tatatatgtg actgacttcg tcagtcctat ctacaacctc
      481 aaaacagtgt tttgagcagc ctgcggctag tttcctagtt tgctctttga ttttcattga
      541 gtattaggga aaaggagatg aatatgaaat ttgggaaacg tcactatcgt ccgcaagtgg
      601 atcagatgga ctgcggtgta gcttcattag ccatggtttt tggctactat ggtagttatt
      661 attttttggc tcacttgcga gaattggcta agacgaccat ggatgggacg acggctttgg
      721 gcttggtcaa ggtggcagag gagattggtt ttgagacgcg agccattaag gcggatatga
      781 cgctttttga cttgccggat ttgacttttc cttttgttgc ccatgtgctt aaggaaggga
      841 aattgctcca ctactatgtg gtgactgggc aggataagga tagcattcat attgccgatc
      901 cagatcccgg ggtgaagttg actaaactgc cacgtgagcg ttttgaggaa gaatggacag
      961 gagtgactct ttttatggca cctagtccag actataagcc tcataaggaa caaaaaaatg
     1021 gtctgctctc ttttatccct atattagtga agcagcgtgg cttgattgcc aatatcgttt
     1081 tggcaacact cttggtaacc gtgattaaca ttgtgggttc ttattatctg cagtctatca
     1141 ttgataccta tgtgccagat cagatgcgtt cgacactagg gattatttct attgggctag
     1201 tcatcgtcta catcctccag caaatcttgt cttacgctca ggagtatctc ttgcttgttt
     1261 tggggcaacg cttgtcgatt gacgtgattt tgtcctatat caagcatgtt tttcacctcc
     1321 ctatgtcctt ctttgcgaca cgcaggacag gggagatcgt gtctcgtttt acagatgcta
     1381 acagtatcat cgatgcgctg gcttcgacca tcctttcgat tttcctagat gtgtcaacgg
     1441 ttgtcattat ttcccttgtt ctattttcac aaaataccaa tctctttttc atgactttat
     1501 tggcgcttcc tatctacaca gtgattatct ttgcctttat gaagccgttt gaaaagatga
     1561 atcgggatac catggaagcc aatgcggttc tgtcttcttc tatcattgag gacatcaacg
     1621 gtattgagac tatcaagtcc ttgaccagtg aaagtcagcg ttaccaaaaa attgacaagg
     1681 aatttgtgga ttatctgaag aaatccttta cctatagtcg agcagagagt cagcaaaagg
     1741 ctctgaaaaa ggttgcccat ctcttgctta atgtcggcat tctctggatg ggggctgttc
     1801 tggtcatgga tggcaagatg agtttggggc agttgattac ctataatacc ttgctggttt
     1861 actttaccaa tcctttggaa aatatcatca atctgcaaac caagcttcag acagcgcagg
     1921 ttgccaataa ccgtctaaat gaagtgtatc tagtagcttc tgagtttgag gagaagaaaa
     1981 cagttgagga tttgagcttg atgaagggag atatgacctt caagcaggtt cattacaagt
     2041 atggctatgg tcgagacgtc ttgtcggata tcaatttaac cgttccccaa gggtctaagg
     2101 tggcttttgt ggggatttca gggtcaggta agacgacttt ggccaagatg atggttaatt
     2161 tttacgaccc aagtcaaggg gagattagtc tgggtggtgt caatctcaat cagattgata
     2221 aaaaagccct gcgccagtac atcaactatc tgcctcaaca gccctatgtc tttaacggaa
     2281 cgattttgga gaatcttctt ttgggagcca aggaggggac gacacaggaa gatatcttac
     2341 gggcggtcga attggcagag attcgagagg atatcgagcg catgccactg aattatcaga
     2401 cagaattgac ttcggatggg gcagggattt caggtggtca acgtcagaga atcgctttgg
     2461 cgcgtgctct cttgacagat gcgccggtct tgattttgga tgaggcgact agcagtttgg
     2521 atattttgac agagaagcgg attgtcgata atctcattgc tttggacaag accttgattt
     2581 tcattgctca ccgcttgact attgctgagc ggacagagaa ggtggttgtc ttggatcagg
     2641 gcaagattgt cgaagaagga aagcatgctg atttgcttgc acagggaggc ttttacgccc
     2701 atttggtcaa tagctagaaa gaggagagga tgaaaccaga atttttagaa agtgcggagt
     2761 tttataatcg tcgttaccat aatttttcca gtagtgtgat tgtacccatg gcccttctgc
     2821 tcgtgttttt acttggcttt gcaactgttg cagagaagga gatgagtttg tccactagag
     2881 ctactgtcga acccagtcgt atccttgcaa atatccagtc aactagcaac aatcgtattc
     2941 ttgtcaatca tttggaagaa aataagctgg ttaagaaggg ggatcttttg gttcaatacc
     3001 aagaaggggc agagggtgtc caagcggagt cctatgccag tcagttggac atgctaaagg
     3061 atcaaaaaaa gcaattggag tatctgcaaa agagcctgca agaaggggag aaccactttc
     3121 cagaggagga taagtttggc taccaagcca cctttcgcga ctacatcagt caagcaggca
     3181 gtcttagggc tagtacatcg caacaaaatg agaccatcgc gtcccagaat gcagcagcta
     3241 gccaaaccca agccgaaatc ggcaacctca tcagtcaaac agaggctaaa attcgcgatt
     3301 accagacagc taagtcagct attgaaacag gtacttcctt ggccggtcag aatctagcct
     3361 actctcttta ccagtcctac aagtctcagg gcgaggaaaa tccccaaact aaggttcagg
     3421 cagttgcaca ggttgaagca cagatttctc agttagaatc tagtcttgct acttaccgtg
     3481 tccagtatgc aggttcaggt acccagcaag cctatgcgtc agggttaagc agtcaattgg
     3541 aatcccttaa atcccaacac ttggcaaagg ttggtcagga attgaccctt ctagcccaga
     3601 aaatcttgga ggcagagtca ggtaagaagg tacagggaaa tcttttagac aaggggaaaa
     3661 ttacggcaag tgaggatggg gtgcttcatc ttaatcctga gaccagtgat tctagcatgg
     3721 ttgcagaagg tgccctacta gcccaacttt atccatcttt ggaaagagaa gggaaagcca
     3781 aactcacagc ctatctaagt tcaaaagatg tagcaagaat caaggtcggt gattctgttc
     3841 gctatactac gactcatgat gccgggaatc aactttttct agattctact attacaagta
     3901 ttgatgcgac agctactaag actgagaaag ggaatttctt taaaatcgag gcggagacta
     3961 atctaacttc ggagcaggct gaaaaactta ggtacggggt ggaaggccgt ctacagatga
     4021 ttacgggcaa gaaaagttat ctacgttatt atttggatca atttttgaac aaagagtaat
     4081 gttcgtgttt ttagagttaa ataattttta aactgtgaga aagattcttc ttgcagtttt
     4141 ttctttacaa tttttgaaaa acatctgcta tttattcggt taaattcttg tgttttttgg
     4201 ttttttgtgg taaaatgtgc tcaagtaata cgaaaggcga actttaaaat gtcaaaacaa
     4261 ttgatctatt cgggaaaagc taaagatatc tatacaactg aggatgaaaa tcttattatt
     4321 tcaacttaca aggaccaggc gactgctttc aatggtgtca agaaggagca gattgcaggt
     4381 aagggagtct tgaataatca gatttcatct tttatttttg agaaattaaa tgcggctggt
     4441 gtggcgactc actttgtgga gaaactttca gacacggaac aactcaataa aaaggttaag
     4501 attattcctt tggaagtcgt gctccgcaac tatactgctg gttccttttc aaaacgtttt
     4561 ggtgtggatg agggaatcgc cttggagact ccgattgtcg aattttacta caaaaatgat
     4621 gatttggatg atccatttat caatgatgag catgtgaaat tcctacagat tgcggatgac
     4681 cagcagattg cctacttgaa ggaagaaacg cgtcgtatca atgaactatt gaaagtctgg
     4741 tttgctgaga ttgggcttaa attgattgac tttaagctag agttcggttt tgacaaggat
     4801 ggcaagatta tcttggcaga cgaattttca ccagataact gccgcttgtg ggatgctgat
     4861 ggcaaccaca tggataagga tgttttccgt agaggattgg gagaactaac cgacgtttat
     4921 gagattgttt gggaaaagtt gcagggttta aaataacaac ctcaaggctg tttgggaata
     4981 ttgcaagagc tgaaataaag gaataagaat tgatggataa acgtattttt gttgaaaaaa
     5041 aggctgattt tcaggtcaag tcagagagtt tggttagaga gctccagcac aacttgggac
     5101 tgtcaagctt gaaaagtatt cgtattgtgc aagtatatga tgtatttgac ttggttgagg
     5161 acttgtttgc acctgcagag aagcacattt tctctgagca ggtaaccgac catgttttag
     5221 atgaagtatc tgtgcaggcg gatcttgcta actatgcttt ctttgccatt gaaagtctac
     5281 cagggcagtt tgaccagcgt gcagcttcgt cacaggaagc cttgcttttg ttgggaagtt
     5341 cgagtgacgt gacagtcaac acagcccaac tttacttggt gaataaagat attgatgcga
     5401 ctgagttgga agctgtcaaa aactacctgc tcaatccagt tgattctcgt ttcaaggata
     5461 tcacgatagg gattgccaag caggagtttt cagagtcaga caagaccatt ccaaaattgc
     5521 ctttctttga aagctataca gcagaagact ttgctcgcta caaggccgaa caagggatgg
     5581 ccatggaagt ggatgatttg ctctttatcc aagactactt taagtcaatc gggcgcgtgc
     5641 caactgagac tgaactcaag gttttggaca cttactggtc tgaccactgc cgtcatacga
     5701 cttttgagac agagttgaaa cacatcgact tttcagcttc taaatttcaa aagcaattgc
     5761 agtcaaccta tgacaagtat attgccatgc gcgaggaatt aggtcggtct gaaaaaccac
     5821 aaaccttgat ggatatggcg actattttcg gtcgttatga gcgtgctaat ggacgattgg
     5881 atgatatgga agtctctgac gaaatcaatg cctgctcagt tgaaattgaa gtggacgttg
     5941 atggtgtcaa ggaaccttgg ctcctcatgt ttaaaaacga aacccacaac catccaacag
     6001 aaattgagcc atttggtgga gcggctacct gtatcggtgg agctattcgt gatccgttgt
     6061 caggccgttc ctatgtttac caagccatgc gtatttcagg tgctggtgat attacagcac
     6121 cgatttcgga aactcgtgct gggaaattgc cacaacaagt catttctaaa acagcaactc
     6181 atggttattc ttcatatggt aaccagattg ggcttgccac aacctacgtt cgtgaatact
     6241 tccacccagg ctttgtagct aaacgtatgg aacttggtgc cgttgttggt gcggctccca
     6301 agggcaatgt tgtccgtgaa aaacctgaag caggtgatgt gatcatcctt ctcggaggta
     6361 aaacaggtcg tgatggtgtc ggtggtgcga cgggctcttc taaggttcaa acagttgagt
     6421 ctgtagagac tgctggtgct gaggttcaaa aaggaaatgc catcgaagaa cgcaagattc
     6481 agcgcctctt ccgtaatggc aatgtcactc gtctgatcaa gaagtccaat gactttgggg
     6541 caggcggtgt ctgtgtggct atcggtgaat tggcagacgg tcttgaaatc gacctcaaca
     6601 aggtgcctct taaataccag ggcttgaatg gtacagaaat tgccatctct gaatcacaag
     6661 aacggatggc ggtcgtggtt cgtcctgaag atgtggatac cttcgttgcc gaatgtaaca
     6721 aagaaaatat tgatgctgtt gtggtggcga cagtaactga aaaaccaaat cttgtcatgc
     6781 actggaatgg tgagacaatc gttgacttgg agcgtcgttt ccttgacacc aatggtgtgc
     6841 gcgtggttgt cgatgccaaa gttgtggaca aggatgtcaa actcccagaa gagcgtcaaa
     6901 catctgctga aacactggaa tcagataccc ttacggttct atctgacctc aaccatgcaa
     6961 gtcaaaaagg attacagact atctttgact gctctgttgg acgctcaacg gttaatcacc
     7021 cacttggtgg tcgttaccaa ctcacaccaa ctgaggcatc tgtgcagaaa ttgccagttc
     7081 aacacggtgt gactcatact gcgtcggtca ttgctcaagg tttcaaccca tatgtagctg
     7141 aatggtctcc ataccacggt gctgcttatg cggttatcga agcaactgct cgtttggtgg
     7201 ctgctggtgc caactggtct aaggctcgtt tctcttacca agaatatttc gagcggatgg
     7261 acaagcaagc agagcgtttt ggtcagccag tagccgccct tctaggctct attgaagcac
     7321 aaattcagct tggcttgcca tctatcggtg gtaaggactc catgtctggt acctttgaag
     7381 aattgaccgt tccgccaacc ttggttgcct ttggggtgac gacggcagat agccgtaagg
     7441 tgctctctcc agaatttaaa gctgttgggg aaaatatcta ctacatccca ggtcaagccc
     7501 tctctgcaga gattgatttt gacttgatta agaaaaattt tgctcagttt gaagcaagcc
     7561 aagctgacca taaagtgaca tctgcatcag ctgtcaaata cggtggtgta gttgaaagtt
     7621 tggctcttgc tacctttgga aactatattg gtgcagaggt gaccttgcct gaacttaaaa
     7681 cagctttgac agctcaatta ggcggctttg tcttcacatc tcctgaagaa attgctggag
     7741 tagagaagat tggacaaacg aaagcagact ttacactgac tgtcaacggt gtgaagctag
     7801 atggacacaa gcttgacagt gcatttcaag ggacattgga agaagtttac ccaacagaat
     7861 ttacccaagc gaaagaacta gaagaagtac cagctgtggt atcagatgtt gtgattaaag
     7921 ccaaagaaaa ggttgaaaaa cctgtggttt acatcccagt ctttccagga accaactcag
     7981 aatatgattc agctaaggcc ttcgaaaaag aaggtgcaga ggtcaatttg gtgccattcg
     8041 tgaccttgaa tgaagaagct attgtcaagt cagttgaaac tatggttgac aatatcgaca
     8101 agactaatat tctcttcttt gccggtggat tctcggctgc ggatgaacca gatggttcag
     8161 ctaagtttat cgtcaatatc ctgcttaatg aaaaagtgcg tgtggctatt gatagcttta
     8221 tcgcccgtgg tggcttgatt atcggtattt gtaatggatt ccaagcctta gtcaaatcgg
     8281 gtctcctacc atacggaaac tttgaagatg ctaacagtac tagtccaacc ctcttctaca
     8341 atgatgccaa ccaacacgtg gccaagatgg tggaaactcg tattgccaat accaactcac
     8401 catggttggc tggtgtgcaa gtgggcgata tccacgctat tcctgtttcg cacggtgaag
     8461 ggaagtttgt cgtgacggct gaggaatttg cagagctccg tgacaatgga caaattttca
     8521 gccaatacgt tgactttaac ggtaaaccaa gtatggattc taagtacaat ccgaatggtt
     8581 ctgtccatgc catcgaagga attaccagca agaatggtca aatcatcggt aagatgggcc
     8641 actcagaacg ttatgaggat ggtcttttcc aaaatatccc aggcaataaa gaccaacacc
     8701 tgttcgcatc agcggttaaa catttcactg gaaaataaga cttacagatt ttctaataga
     8761 tagtatcagt aatgtaaaag tcatgtaaat ctagctcttg atgattacaa atgaaaatta
     8821 ggtataaaaa atgacatacg aagtaaaatc tcttaatgaa gaatgtggtg ttttcggtat
     8881 ttggggacat ccagatgctg ctaagttgac ctattttgga ctccacagtc ttcaacaccg
     8941 tggtcaggag ggggcaggaa tcctctccaa tgatcaagga caactgaagc gccatcgtga
     9001 catggggctt ttatcagaag ttttcagaaa tccagccaat ttggataaat tgacaggagc
     9061 tggtgcgatt gggcatgtgc gttatgcgac tgctggcgaa gcttctgttg ataacatcca
     9121 acccttcctc ttccgttttc acgatatgca gtttggtttg gctcataatg gaaatctgac
     9181 caatgcagcc tctctcaaga aagaactgga acaaagagga gcaattttca gcgcgacttc
     9241 ggactctgaa atcttggctc acctcattcg tcgcagtcat aatcctagcc tgatgggcaa
     9301 aatcaaggaa gcgctcagtc ttgtcaaagg tggttttgcc tatatcttgc tgtttgagga
     9361 caagttgatt gcggctcttg accctaatgg ctttcgtccg ctttctatcg ggaaaatggc
     9421 caacggagcg gtggttgttt cctctgaaac ctgtgctttt gaggtcattg gtgccgagtg
     9481 gatttgtgat ttgaagccag gtgagattgt gatcattgat gacgagggca ttcagtatga
     9541 cagctataca gatgataccc agttggcgat ttgttctatg gagtatatct actttgcccg
     9601 ccctgattct aatatccacg gtgtcaatgt ccatacggca cgtaagagaa tgggagcgca
     9661 attggcgcga gaatttaagc atgaggcaga tattgtagtt ggtgtgccca attcttccct
     9721 aagcgcggct atgggatttg cggaagaatc aggcttacca aatgaaatgg gtctgatcaa
     9781 aaaccaatac acccagcgaa cttttatcca accgactcaa gaattgcggg agcaaggagt
     9841 gcggatgaaa ctgtctgctg tttcgggtgt tgtcaaaggc aaacgtgtgg tcatggtgga
     9901 tgattccatt gtacgtggaa caacctctcg tcgtatcgtt cagctcttga aagaagcggg
     9961 tgcgactgag gttcacgttg ccattggaag tcctgcacta gcgtatccat gtttctacgg
    10021 gattgatatc cagacccgtc aggagctgat tgcagccaat catacggtcg aagaaactcg
    10081 ccaaatcatt ggtgcggaca gtctgactta tctttcaatt gatggcttga ttgagtcgat
    10141 tggtatcgaa acagatgcgc cgaacggtgg tctctgtgtc gcttactttg acggtgacta
    10201 cccaacgcct ctctatgact acgaagaaga ctatcgtaga agtttggaag aaaagaccag
    10261 tttttacaag taggcgacag attctccatt aaagaaaagg aaaaaacaaa tgacaaataa
    10321 aaatgcatat gcctcacgtc tcactactga ctaaaggctt aagcatttag tcagtagacg
    10381 ctttgtccta taggatcaaa gctagagccc tgactagtat ttttagataa aaagatggtt
    10441 tatctaaaaa tacgtcgcag tctttctcaa aaaaagaaaa ggaaaaataa aatggcaaat
    10501 aaaaatgcgt acgctcaatc tggtgtggat gttgaagcgg gttatgaagt tgttgaacgg
    10561 attaaaaagc acgtggctcg tacggagcgt gcaggtgtca tgggagctct tggtggcttt
    10621 ggtggtatgt ttgacctttc caagactggg gttaaagaac ccgtcttgat ttcagggact
    10681 gacggtgtcg gaaccaagct catgttggct atcaagtacg acaagcacga taccatcggg
    10741 caggactgtg tggccatgtg tgtcaacgac atcattgctg caggtgcgga acccctctat
    10801 tttctcgact acgtagcgac agggaagaat gaaccagcta agctagaaca agtggttgct
    10861 ggtgtggcag aaggttgtgt gcaggctggt gctgccctca tcgctgggga aacggctgaa
    10921 atgccgggca tgtacggcga agacgactat gacttggctg gttttgcggt cggtgtggct
    10981 gaaaaatctc aaatcattga cggttcaaag gtggtagagg gagatgttct tctcggactt
    11041 gcttcaagtg ggattcactc aaatggttac tctttggttc gtcgtgtctt tgcggattac
    11101 acaggtgagg aagtcctacc agaattggaa ggcaagaaac ttaaggaagt tctacttgag
    11161 ccgactcgta tctatgtcaa ggctgtcttg ccgctcatca aagaagagtt ggtcaacggc
    11221 attgcccaca tcacaggtgg tggctttatc gaaaatgtcc ctcgtatgtt tgcagatgac
    11281 ctagctgctg aaattgatga aagcaaagtt ccagtgcttc caattttcaa agcccttgaa
    11341 aaatatggtc agattaaaca cgaagaaatg tttgaaatct tcaatatggg tgtgggactt
    11401 atgttggcgg tcagccctga aaatgtagag cgtgtaaaag aattgttgga tgaagcagtc
    11461 tatgaaattg gtcgcatcgt caagaaagaa aacgaaagtg tcattatcaa atgaaaaaaa
    11521 tagcggtttt tgcctctggt aatggctcaa attttcaggt gattgccgaa gaatttccag
    11581 tggagtttgt cttttcagac catcgtgatg cctatgtgct tgagcgtgca aagcagctcg
    11641 gcgttctgtc ctatgctttt gaactcaagg agtttgagag caagacagac tacgaagcag
    11701 cccttgtcga actcttggaa gaacaccaga ttgacttggt ttgcctagca ggctacatga
    11761 aaatcgttgg accaacctta ttgtcggctt atgaaggtcg gattgtcaac attcatccag
    11821 cctacttgcc agaatttcca ggagctcatg ggattgagga tgcttggaat gctggcgtgg
    11881 gtcagtctgg tgtgaccatt cactgggtgg attcgggtgt ggatacaggc caggtcatca
    11941 aacaggttcg tgtgccacga ctagctgatg ataccattga cagatttgaa gctcgcatcc
    12001 atgaagcaga gtacaggttg tatccggaag tagtgaaggc tctatttaca gattgacttt
    12061 ttgatgattc atatgatatc tttgatttta aattggagtc agtgtttgtt gaagacggct
    12121 tcaaacggag gtatttgtaa tgttagaatc taaaaaaaca actcgatatg tattttatgt
    12181 ctatctgatg ttattaactt ggggaatctt atttaagttt gaaacaaatc ctgaatttat
    12241 agcatttttc ttagctccaa ggtatatcaa ttggattcca ttttcagaac cactaatagt
    12301 cgatggaaaa attgtttttg ctgaaatgtt atttaatctg atttccttta ttccattagg
    12361 tgtttgtttc cctttgataa aaactaattt atctagttta agaatagtcg ggacaggttt
    12421 cttgattagt ttattgtttg agtgcttaca gtatatttta gcaataggta taacagatat
    12481 aacggatttg actttaaata cgctaggtgt ctgtgtaggc ttactgattt atcaaatttt
    12541 tataagagtg ttcaaatcac agactagaaa atggatcaat atcttaggta tgcttagcct
    12601 tggttttgct tatcttgttt tactgttact gcatttaatt ggtgtttaac taatgattaa
    12661 aaaggagaat ataatgacta aacgcgtctt aatcagcgtc tcagacaaag cgggcattgt
    12721 tgaatttgcc caagaactca aaaaacttgg ttgggagatt atctcaacag gtggaactaa
    12781 ggttgccctt gataatgctg gggtggatac cattgctatc gatgatgtta ctggtttccc
    12841 agaaatgatg gacggtcgtg tgaagaccct ccacccaaat atccacggag ggcttctcgc
    12901 tcgtcgtgac ttggatagcc acttggaagc ggctaaggac aacaagattg agctcattga
    12961 ccttgtggtg gtcaaccttt acccatttaa ggaaactatc cttaaaccag atgtgactta
    13021 tgctgatgca gttgaaaata tcgatattgg tgggccgtct atgcttcgtt cagcagcgaa
    13081 aaatcatgcc agtgttacag ttgtggtaga tcctgctgac tacgctgtgg ttttggacga
    13141 attggcagca aacggcgaaa cctcttatga aactcgccaa cgtttagcag ccaaagtatt
    13201 tcgtcacaca gcggcttatg acgccttgat tgcagaatac ttcacagctc aagtgggtga
    13261 aagcaagcct gaaaaactca ctttgactta tgacctcaag caaccaatgc gttacggtga
    13321 gaatcctcaa caagacgcgg acttttacca gaaagctttg cctacagact actccattgc
    13381 ttcagccaaa cagctcaacg ggaaagaatt gtcatttaat aatatccgtg atgcagatgc
    13441 tgctatccgt atcatccgtg acttcaaaga tagtccaacc gttgtggctc tcaaacacat
    13501 gaaaccatgt ggaattggtc aagctgatga catcgagact gcttgggact acgcttatga
    13561 gtctgaccca gtgtctatct ttggtgggat tgtcgtcctc aaccgtgagg tggatgctgc
    13621 gacagctgag aagatgcacg gcgttttcct cgaaatcatc attgcaccaa gctatacgga
    13681 tgaagcgcta gccattttga tcaataaaaa gaaaaacttg cgtatccttg ccttgccatt
    13741 taatgctcaa gaggctagcg aagtggaagc agaatacaca ggtgtagtcg gtggacttct
    13801 cgtgcaaaat caagacgtgg tcaaggaaag cccagctgac tggcaagtgg tgactaaacg
    13861 tcagccaact gagacagaag cgactgctct tgagttcgct tggaaggcta tcaagtacgt
    13921 caaatcaaat ggtattatcg tgaccaacga ccacatgaca cttggtgttg gtccaggtca
    13981 aaccaaccgt gtggcttctg ttcgccttgc cattgaccaa gccaaagatc gtctggacgg
    14041 ggcggtcctt gcttcagatg ccttcttccc atttgcggat aacgtggaag aaatcgccaa
    14101 agcaggaatt aaggccatca tccagcccgg tggctctgtc cgtgaccaag aatccatcga
    14161 agcagcggat aaatacggct tgactatggt ctttacaggt gtgagacatt ttagacatta
    14221 agaagataaa agggaagaaa acagtttctt tccttttttg gcttaaaatg ctaactgaaa
    14281 caagattaaa acgaactttt ttgatataat gttggtaaat aattcgcaaa agaggttgag
    14341 gaatgaaact gcttgttgtc ggttctggtg gtcgtgagca tgcgattgct aaaaagttac
    14401 ttgaatcaaa agacgtggaa aaagtctttg tagctcctgg gaatgatggg atgactctgg
    14461 atggtttgga attggtaaat atctctattt ccgaacattc taaattgatt gacttcgcaa
    14521 agaccaatga tgttgcttgg acctttatcg gtccagatga tgcccttgct gctggcatcg
    14581 tggatgattt taaccaagct ggacttaagg cctttggtcc gactagggct gcagcggagc
    14641 tggagtggtc caaggatttc gccaaggaaa tcatggtcaa atacggcgtt ccgacagcaa
    14701 tatatggcac attttcagat ttcgaggaag ccaaggccta tatcgaaaag catggtgcgc
    14761 ctatcgtagt caaggcggat ggcttggcac ttgggaaggg tgtcgtcgtt gctgagacgg
    14821 ttgagcaagc ggtcgaagcc gctcatgaga tgcttttgga caataaattt ggtgactcag
    14881 gtgcgcgcgt ggttattgag gaattccttg aaggcgagga attttcactc tttgcctttg
    14941 tcaatggtga taagttctac atcatgccaa cggctcagga ccacaaacgt gcctatgatg
    15001 gcgacaaagg gcctaacacg ggtggtatgg gtgcctatgc gccagttcca cacttaccac
    15061 agagtgtagt tgatacagcg gttgacacca ttgtcaagcc agttctagaa ggggtgatta
    15121 aagaaggtcg tccttatctg ggagttcttt acgcagggct tatcctgaca gctgatggac
    15181 cgaaggtcat cgagttcaac gctcggttcg gagatccaga aactcagctt atcttgcctc
    15241 gcttaacctc tgactttgct caaaatatca cagatatcct ggatagcaag gagccaaata
    15301 tcacgtggac ggacaagggt gtgactctgg gtgtggttgt cgcatccaag ggctacccgc
    15361 tagattattc aaagggtgtt gagttgccag tcaaaaccga tggcgacatc attacctact
    15421 atgcaggggc taagtttgcg gaaaatagca gagcactgct ctcaaacggc ggacgagttt
    15481 atatgctcgt taccacagca gataccgtca aagaagccca agccagcatc taccaagaac
    15541 tatcccaaca aaaaatagaa ggactcttct accgaacaga tatcggaagc aaggcaatta
    15601 agtaaagata taagaataac gcgccgtagt cgccaaacac gataatggtc gtcgtggtga
    15661 aaagaccaga acagtgaatg ttctggtcag ggggaaactt ggagaccgta ggctcaaagt
    15721 ttaggaatga aaccgaaggt ttgcttccgc ctccatcacc taagaccatt atcaaaaaga
    15781 aaaataaaaa ttcacaaaat acgttaatgg tcgtatggtt tgcgagcgtt agcgagctaa
    15841 tatagaataa tcaccgccgt tgtgaaagaa cgattggatg acaatccaat cgttcaggga
    15901 aattggaaga ccttgggttt ccaatttagg catgagacac ctttggtggt tgctgccgtc
    15961 cctcacaagc taaggtgatt gttgaaaaag aggaaaaagg agaagaaatg aaaccagtaa
    16021 tttccatcat catgggctca aaatccgact gggcaaccat gcaaaaaaca acagaagtcc
    16081 tagaccgctt cggtgtagcc tacgaaaaga aagttgtttc cgcacaccgt acaccagacc
    16141 tcatgttcaa acatgcagaa gaagcccgta gtcgtggcat caagatcatc atcgcaggtg
    16201 ctggtggcgc agcgcatttg ccaggcatgg tagctgccaa aacaaccctt ccagtcattg
    16261 gtgtgccagt caagtctcgt gctcttagtg gagtggattc actctattct atcgttcaga
    16321 tgccgggtgg ggtgcctgtt gcgaccatgg ctatcggtga agctggagcg actaacgcag
    16381 ctctctttgc cctccgtctc ctctctgtag aagataagtc cattgcggat gcacttgcca
    16441 actttgctga agaacaagga aaaatcgcag aggagtcgtc aaatgagctc atctaaaaca
    16501 atcggaatta tcggtggcgg tcaactgggt cagatgatgg ccatttctgc tatctacatg
    16561 ggccacaagg ttatcgcgct ggatcctgcg gcggattgcc cggcctctcg cgtggcggaa
    16621 atcattgtgg caccttataa cgatgtggac gccctccgtc agttggcaga ccgttgcgat
    16681 gtcctcactt acgagtttga aaatgtcgac gctgacggtt tggatgccgt tatcaaggat
    16741 ggacaactcc ctcaaggaac agatctgctc cgcatttcgc aaaatcgtat ttttgaaaag
    16801 gactttttgt caaacaaggc tcaagtcact gtggcaccct acaaggtcgt gacttctagc
    16861 ctagacttgg cagatatcga cttgtcgaaa aactatgtcc tcaagactgc gactggtggc
    16921 tacgatggtc atggacaaaa ggttattcgt tcagaagcag acttggaagc agcctatgcg
    16981 ctagcagact cagcagactg cgtcttggaa gaatttgtca actttgacct tgagatttct
    17041 gtcatcgtgt caggaaatgg caaggaggtg acgtttttcc cagttcagga aaatatccac
    17101 cgcaacaata tcctgtctaa gaccatcgta ccagcccgca tttctgaaag tctagtagac
    17161 aaggctaaag ctatggcagt gcgaatcgca gaacaactca acttgtctgg aactctctgt
    17221 gtggaaatgt ttgcgactga tgatgacgtt attgtcaatg aaatcgcccc acgaccacat
    17281 aactctgggc actattctat tgaagcctgt gatttctctc agtttgacac ccatattctc
    17341 ggtgttctcg gagcaccatt accagtcatc aaactccatg cgccagccgt tatgcttaat
    17401 gtcctcggtc agcatgtcga ggctgctgag caatatgtga cagaaaattc aagcgcccac
    17461 ctccacatgt atggtaaaat agaagcgaag cacaaccgca agatgggaca tgtgaccttg
    17521 tttagtgatg taccggatag tgtggatgag cttggggaag ggattgattt ttaggacaag
    17581 tctatgatac aaattatcgt taatacattt attgaaaagg ataagactgg agcagttgtt
    17641 gaagtgttgt atgccagtgc tgaccaagat aaggtacaag ctaaatatga agaactagct
    17701 gcataatacc ccgaaaatta tttagctatc tatgatgtac cgctggatac ggatttgaat
    17761 acactagatc attacccgtc tgtgtttatt gaaaaagagg agtttgagta gaaatcttgg
    17821 tttacctaga tagcttattc ccaacagctt aagaagaaag gaaaaattaa cacatgatca
    17881 accgttactc tcgccctgag atggcgaata tttggagtga agaaaataaa taccgtgctt
    17941 ggcttgaggt ggaaatcctc tctgacgagg catgggctga gttgggggaa atccctaagg
    18001 aagatgtggc tttgattcgc aagaaggcgg actttgacat cgaccgtatt ttggaaattg
    18061 agcaggagac gcgccacgat gtggtggctt tcacgcgtgc ggtttctgag actcttggtg
    18121 aagagcgcaa gtgggttcac tatgggttaa cttctaccga cgtggtggat actgcttatg
    18181 gttacctcta caagcaggcc aacgacatca tccgtcgtga ccttgaaaac ttcactaata
    18241 tcatcgctga caaggccaag gagcacaagt tcaccatcat gatggggcgt actcatggtg
    18301 tgcacgctga gccgacaacc tttggtctta aattggcaac ttggtacagc gaaatgaaac
    18361 gcaatatcga gcgtttcgag catgcagctg ctggtgtaga agctggtaag atttctggtg
    18421 cggttgggaa ctttgccaat atcccaccat ttgtagagga gtatgtctgc gataaacttg
    18481 gcatccgtgc ccaagaaatc tctacacaag tccttcctcg tgaccttcac gctgagtact
    18541 ttgcggttct tgccagcatt gcgacttcaa tcgaacgtat ggcgactgag attcgtggtc
    18601 tacaaaaatc tgagcaacgc gaagtagaag agttctttgc taaagggcaa aaagggtctt
    18661 cagcaatgcc tcacaaacgc aacccaatcg gttctgaaaa tatgactggt ctggcgcgtg
    18721 tcattcgtgg tcacatgatt acggcttatg aaaacgtcgc tctctggcac gaacgcgata
    18781 tttctcactc atcagctgag cgtatcatca caccagatac gaccattttg attgactaca
    18841 tgctcaaccg ttttggaaat atcgtcaaga acttgacagt cttcccagaa aatatgatcc
    18901 gaaacatgaa ctcgactttt ggtcttatct ttagccaacg ggctatgttg acattgattg
    18961 aaaaaggcat gacccgtgag caagcctatg acttggtgca accaaaaaca gcctactctt
    19021 gggacaacca agtagacttt aaaccacttc ttgaggcaga ttcagaagta acatcacgtc
    19081 tcactcaaga agaaatcgat gaaatcttca acccagttta ttacaccaaa cgagtggatg
    19141 atatctttga acgtcttgga ctaggtgatt aattaaaaaa taaacagcga gcttcaatct
    19201 cgctgtttat tttttatcga aaagacttag tcttcttttc ttttagtgag tccataggct
    19261 gctagtgtgg acatgagtcc tgcgactact agtcctgcag aatcgtgagt tcctgtttca
    19321 ggaagttttt tctctgttac cacaggagct ggatcttgag gaagagcttt gctttcctca
    19381 gcaggagcag ttgatggagc tggttggctt gggatttcta gttttggttt ttcttcagca
    19441 atagcggctt gtccgttttc atcgcctaca tgtgttacca tagttccgac ttcgactatt
    19501 tgagtaacgg cttcctgtgc tacgacacta tttacaagtg ttttcacttc cttaccatcg
    19561 gcagaagtgc tcacagagta gaagttgcta cgatgtccat tgacgccctt agtaatgact
    19621 tgtgtttttc ctttgagtaa gagtggattt tcacgagtca ctgtggtaaa tggaatttct
    19681 tctttttgga tatccagtct aggttttacc tcagtagttg gtgcaagacc actttcatca
    19741 cccttgtgag ttacaggagc gccaacttca accacttggt ttataacttc tttggttacc
    19801 tggctatcaa ggactgtttc tgttgttttt ccattttcag tgagtacaga gatgtaatga
    19861 gttcgttcac ctttgactcc tgctgtgata atattttctt gaccagctgg gaggttagga
    19921 ttttctttct tgataacttc aaatggaatt tcttcagttc ttgtgatgag ttctggtctg
    19981 gtttcaacat tggcagccac ttcattttca tctaggcttc ctgaatgagt tgcagctggt
    20041 ttgaggcctt gaagagcggc ttttaggttg gctacaagtg tgtcaagctc agcttgttta
    20101 ttacggttga ggttgtaatt tagagctgtt ttagctgcgt caagggcctc aagactttct
    20161 ttactatatc cttctaagtt tgtaggaatt ttagctaatt cttcgcggag agcattataa
    20221 ttagcacgga agtagtcttt gttgtggtct gcaaaggcag tcatgagttc aaagatttcc
    20281 tcttccttgt attcagcgct tggtctatct gcccagattg aaagcatact tccgactgtt
    20341 ggaagatcta cttcaggata tttggtagaa gctagttgat tgaatggaac tttaccagaa
    20401 ttttcaactg cttttgaaag agggtaagct tcctcttgtt tgtgattgcc tataacataa
    20461 taccagtcac cgttggtatt caagaattta tagcctttgc ttgctaggta ttgaggtgat
    20521 gcgaggttat atccccacca gcctttagac cagtaagaaa tcaagacatc tttgtcaaac
    20581 tgaacatcgt ccttgtcttc atagtagaag ccatcgttga aggccattgg ttgaagccct
    20641 ctttctttgg ccatagctgc gagggtgttg gcatattcgg caaatttgcc atagagttga
    20701 taccacttga ggtagtacca gccttgggca ctagtcgcat cgttggcgta ttcgtcagta
    20761 ccaaagttga aaatctttgt tttacctgca aagaagtcca tgtatttacc gatgagggct
    20821 tttacaaagt ccatcgcttc ttcgtttttc aagtccatag ttgtttttga aactttatca
    20881 aagtgggctt gaggattttt aatacctaat ttttccatgg caaccagcat agcatccatg
    20941 tgacctggac tgttaatagc tgggatgaga ccgatgtcct tagatttagc gtattcaatt
    21001 agctctgtca cttctgcctg tgttagtgca gtaccgtttg gatcgtcgta gtaagcttta
    21061 gttccttcga taatagcttt tttaacgtca tcactagcat aggtttttcc gttggcagta
    21121 atggtcatat cattgagtag aaagcgaagt ccgtcatttc ctagaaggag atggacatca
    21181 gaatatccga gctcactggc cttgtctacg atgcgtttga gctggttcag agtaaagtat
    21241 ttgcgtccag catcgattga gattaccttg tttttggcaa gtttttcaac ctcacgttta
    21301 gcttcttctt ctttttgagc ttcaggcgtg agggtcaagt tgttgacagt ttcttgaagt
    21361 ttagcaatgg cttgatcaat cgtatcttgt tgggcacggc taaggttgct atcgagagag
    21421 cgaatagctt tttcagcttc ttttacggcc gcgacgcttt ctgcagtata acggttcagg
    21481 tcttttggta cctcgttaag tgcttgctct gcagattcat aatcagctgc gaagtattca
    21541 gcgttggcat ttgcaaaatg acgcatgagt ttgaagaggc gtgatggtga ataacgtgca
    21601 gatggagtgt cagcccaagc agctaccata ccaccgatga ttgggatatc agctccttct
    21661 gtttttggta cagaagtgat tggtgtgttt ttaataccat tgagcccctg atcgagattg
    21721 taccagcctt ggccatcagc gtttcgtcca agaacgtagt accaagcatc attggtatta
    21781 aggatttggt gacctttttc agctagtagt ttagaagaag cgacatcgta gcctccccaa
    21841 ccaccagtcc acatagaaac gatgatgtct ttgtcaaaac taccaaagct tgtgtcgcta
    21901 ttgtagtaga taccgtcgtt aaaggccatt ggtttgagac cgtgcgattt tacaatacga
    21961 gcgaggtcat tggcgtaggc aataaatttt tcatagcctt ttacagggta gccttcgttt
    22021 ggatagtatt tatcagcttg aagcacactc caacctttag catctgtcgc atcattggca
    22081 tattcatcaa gtccgatgtt gaagatttca gtctttttcg cgaaataagc agcatacttg
    22141 tcgataaggg cttttgtaaa agcgacagct tgttcgttgt caagatcgac agtacgggct
    22201 gatttcttcc caaaatagct aaagttaggg ttttggattc ccaattcttt catggcattg
    22261 agaatcgcat ccatgtgtcc aggactattt actgtcggaa tgagaccgat acctttatct
    22321 ttggcatagt taatcagatc tgtcatttga ctttctgtta agtgattgcc gtttggatcg
    22381 ttgtaataat catttgtacc tttttcaatg gcgcgtttga catcgtcact ggcataggtc
    22441 ttgccgttag ctgtgatgct catatcgtcc aacatgaaac ggagtccatc atttccgact
    22501 aataggtgta aatcagtgta gccataatgt ttcgctttat cgatgatttc cttgagctgt
    22561 tctggtgaga aatatttacg tccagcatca atagaaacaa ttttcttttt cgctagtttt
    22621 tcatttacag ttgcagcacg ttcctttcct gcctctgttt ccggtttgtc agcctctgct
    22681 ttcgcttcat cttttttagc tggtttatcc ttgtcagtct tgtctgtatt tgactcttta
    22741 gaatcaacct ctttcgcttc ttccttttta gggctagctt cttctgcctt tttattagca
    22801 gtttcttttt cagcagaagt tggagttacc acttctgctt tatcactagg agttgaacta
    22861 acttcctctt gtggtttttc ttctgttttt ggaagactag ctaccttatc agtagctgga
    22921 gtttctgttt ctacagtttt tggagcttct ggttgaagca ctgctttagg tgtttcctca
    22981 gtccgatttt cggatgattg aggggaatca gaaaccgtat ggatggtcgg ttggttttct
    23041 gtagtagtag gagtaactcc atcggctgca acagtctgtg cttggaaggc aaatccaatt
    23101 agaacagaag ctgctcctac agcgtattta cgaatagaaa aacgctgttg tttttcatgt
    23161 ttcattgcaa aacctcctga ttgcattgtt atattgatag cgattatata aatcaacgcc
    23221 cttattttat ttcttatatt aacgagagtc aagaggagat gacaaaaaac tataataagt
    23281 ataaaaaaat ataaaattta aacttaagat ttcagattgg tcggaaaaaa tgcatatata
    23341 tatctagtat aatttttggt tctatttcta taaaatattc cacaaattat agaatcttcc
    23401 aaaaataggt aagcgctacc tttttggtgt ggtataataa gcatagaaaa agcccaagcg
    23461 attagctcag gttttcttct tagtgatcac ggtcacatga gataaattta atcttgtagt
    23521 aatcagatcg tttgtaagtt tcactgtatt ctaaaacttg gccagttgat tcgagtttgg
    23581 tgattttagt ttgtaggaca gtagggaatt gttcatcgac tccgaggact gaagccgcat
    23641 gttctggagt tggaaagact atttcgttga tttcttcaaa gtgttcatca ttcatgtgaa
    23701 tgtggtagtc taacttgaaa cgattataga tagaactata gtattcaagg tttggataat
    23761 ttgcgttgat atattgttct gggatgtagg atgtatggta gatataaacg acaccgtttg
    23821 attcgcggat acgttcaatc ttgtagtaga attgatcgcc gcgtagaccc aatttttcca
    23881 agtaaacaag cttgtttccg cgttcaattg aaagaacagt taccttatca tctttagcat
    23941 tgaagagctc aatatctgaa aactctacaa gcttgtgttt gcgtgcacgt gaaacgaagg
    24001 ttccttttcc ttgttggcgg acaatatagc catctttggc aaggtcgttt aaggcgcgaa
    24061 caactgtgat agagctgaca tcgtacattg aaatgagttc tgcttcagtg taaaatttat
    24121 ctccactgct aaactgccca gagatgattt tattttttaa ttcgtctttt atgtattgat
    24181 atttgggaat tgccatattt ttacctctat tttccttctc catagaaaat tttaccataa
    24241 aagcgaaaaa aatagtagtc ttttgaataa aaaattagaa taatagtttg aaaaattaag
    24301 cgatataggg attgctcata ttttatcaat gcattctata caaaattatt ttaggtccta
    24361 tattccgaat ttaaagagcg tttgaaatgg gtctaaaaat gtttgaaact aattgaatgt
    24421 gtgtataata gtaaaaatgc agtaaatatc ccgacaattt ctattgacaa ttcaaacaga
    24481 ttggtttata attaatataa caacaaatga aagcgcaaac tttcgcggtc ggaaggtagt
    24541 tttatgacac gatttgagat acgagatgat ttctatctcg atggaaaatc atttaagatt
    24601 ttatctggtg ccattcatta ttttaggatt cctccagagg attggtatca ttcgctctat
    24661 aacttgaagg ctcttggttt taatacggta gagacttatg ttgcttggaa tttacacgag
    24721 cctcgtgaag gtgagtttca ttttgaaggt gatctggatt tagagaaatt tctccaaata
    24781 gcgcaggatt tgggtctcta cgcaattgtg cgtccgtctc catttatctg tgcggaatgg
    24841 gaattcggtg gcttaccagc ttggctcttg accaagaaca tgcgaattcg ctcatccgac
    24901 ccagcatata tcgaggcagt tggtcgctac tatgatcagt tattgccaag actggtgcct
    24961 cgtttgttga acaatggtgg caatattctc atgatgcagg ttgaaaatga gtatggttct
    25021 tacggagaag ataaggctta cctgagagcg attcgacagc taatggaaga gtgtggcgta
    25081 acctgtcccc tctttacatc agatggtcca tggcgagcta ctctgaaagc tggaacctta
    25141 attgaagagg acctctttgt aacaggaaac tttggttcta aggcacctta caacttttcg
    25201 cagatgcagg aattctttga tgaacatggt aagaaatggc cactcatgtg tatggagttc
    25261 tgggatggtt ggttcaatcg ctggaaagaa ccgattatca cacgggatcc taaggaattg
    25321 gcagatgcag ttcgagaggt tttggaacaa ggctctatca atctttacat gttccacggt
    25381 ggtacaaact ttggtttcat gaatggttgc tcagctcgag gaactttgga cctgccacaa
    25441 gttacatctt atgattacga tgcccttctg gatgaagaag gaaatccaac tgctaaatat
    25501 cttgcagtca agaagatgat ggcaacacat ttttcagagt atccgcagtt ggaaccactc
    25561 tacaaagaga gtatggagtt ggatgctatt ccactagttg aaaaagtttc tttgtttgaa
    25621 accttagata gcttgtcaag tcctgtagaa agtctctatc ctcaaaagat ggaggagctg
    25681 ggacaaagtt atggctacct actttatcga acagaaacaa actgggatgc agaagaagaa
    25741 agacttcgta tcattgatgg tcgagatagg gcccagctgt atgtcgatgg tcagtgggtt
    25801 aaaactcaat atcagacaga gattggggaa gatatttttt atcaaggtaa aaagaaaggg
    25861 ctatctaggt tagatatctt gatagaaaat atggggcgtg tcaactatgg gcataagttc
    25921 ttagcggata cgcaacgtaa gggaattcgg acaggggtct gtaaggatct gcatttctta
    25981 ctaaactgga aacactatcc actcccacta gacaatcctg agaaaattga tttttcaaaa
    26041 ggatggactc aaggacaacc agccttttac gcttatgact ttacagtcga agagccaaaa
    26101 gatacttacc tagacttgtc tgagtttggt aagggagttg cctttgtcaa tgggcagaat
    26161 ctaggacgtt tttggaacgt tggcccaact ctctcacttt atatccctca tagctatctc
    26221 aaggaaggtg ccaaccgtat cattatcttt gaaacagaag gtcaatataa agaagagatt
    26281 catttaactc gtaaacctac actaaaacat ataaaggggg aaaacttatg acaattgtag
    26341 gatgccgtat tgatggacgt ttgatccacg gacaagtagc caatctttgg gctggaaaac
    26401 taaatgtttc acgcattatg gttgtagacg acgaagttgt caacaacgat attgaaaaga
    26461 gtggtttgaa acttgcgaca ccaccaggtg tgaaattgag tattttgcca gttgagaaag
    26521 ctgcagccaa tattcttgct ggtaaatacg atagccaacg tctctttatc gtggctcgta
    26581 aaccagaccg cttccttggt ttggtagaag caggtgtacc acttgaaacc cttaatgttg
    26641 ggaatatgtc tcaaacacca gaaactcgtt ctattacacg ttctatcaac gtagtagaca
    26701 aggatgtgga agacttccac aaactggcag aaaaaggtgt taaacttact gctcagatgg
    26761 ttccaaatga tccaatttca gactttttga gcttattaaa ataggaaaaa aatttttagg
    26821 aggtcattgt tatgatacaa tggtggcaaa ttttacttct cactttgtac tcagcttatc
    26881 aaatctgtga tgagttgacg atcgtttcat ctgcaggttc ccctgtattt gctggtttca
    26941 ttactggttt aatcatggga gatgtgacta ctggtttact tatcggtggt aacttgcaac
    27001 tgttcgttct tggggttggt accttcggtg gtgcttctcg tatcgacgca acttctggtg
    27061 cggttcttgc gacagccttc tctgtttcac aaggaattga tgcaccgctt gccattacta
    27121 caatcgctgt accagtagca gctctcttga cttacttcga cgttcttggt cgtatgacta
    27181 ctaccttctt cgctcaccgt gtggatgctg caatcgaacg ctttgactat aaaggtattg
    27241 aacgcaacta cttgcttggt gcgattccgt gggctctatc tcgtgccctt ccagtcttct
    27301 ttgcccttgc ttttggtggt gcctttgtac aatcagtagt agacttcgtt gaagcctaca
    27361 aatgggttgc agatggcttg acacttgcag gacgtatgct tccaggtctt ggatttgcaa
    27421 tcttgcttcg ttaccttcca gttaaacgta accttcacta ccttgctatg ggatttggtt
    27481 tgacagctat gttgactgtt ctttactcat atgtaacagg tcttggtggc gctgttgctg
    27541 gtatcgtagg tactcttcct gctgaagttg ctgaaaaaat tggtttcgtg aacaacttca
    27601 aaggtttgtc tatgattggt atttctatcg taggtatttt ccttgcagtg cttcacttca
    27661 aaaatagcca aaaagtagct gtagcagcac cttctacacc atcagaaagt ggggaaatcg
    27721 aagatgacga attctaatta caaacttaca aaagaagatt ttaatcaaat caacaaacgt
    27781 agcttgttta ctttccaatt aggttggaac tacgaacgta tgcaagcttc tggttacctt
    27841 tacatgatct tgcctcagtt gcgtaaaatg tatggtgatg gaactcctga attgaaagaa
    27901 atgatgaaag ttcatactca attcttcaat acttcaccat tcttccatac cattatcgct
    27961 ggttttgacc ttgccatgga agaaaaagat ggtgtaggtt caaaagacgc cgttaacggt
    28021 atcaagacag gtttgatggg accattcgct cctcttgggg atacaatctt tggttcactt
    28081 gtacctgcta tcatggggtc agtcgcagca actatggcta tcgctggcca accttggggg
    28141 atcttccttt ggattgcagt tgcagtagcg tatgacatct tccgttggaa acagttggaa
    28201 tttgcttaca aagaaggggt taaccttatc aacaacatgc aaagtacctt gacagctttg
    28261 attgacgctg catctgtact tggtgtcttc atgatgggtg ctcttgtagc aacagtgatt
    28321 aactttgaaa tttcttacaa gttgccaatc ggtgaaaaga tgattgattt ccaagacatc
    28381 ttgaaccaaa tcttcccacg tttgcttcca gcaatcttta ctgcctttat cttctggttg
    28441 cttggtaaga aaggtatgaa ctctactaaa gctatcggta ttattatcgt acttgctttg
    28501 gctctttctg cccttggtca ctttgcactt ggaatgtaat tccttatgac taaatcatta
    28561 attttggtga gccatggtcg cttctgtgag gagcttagag gtagcacaga aatgattatg
    28621 ggcccacaag acaacattta cacagtagct cttcttccag aagatggccc agaagaattt
    28681 actgctaaat ttgaagctgt tattgaagga ttggatgatt tcctagtctt tgcggatctt
    28741 ctcggtggga caccttgtaa tgtggtgagt cgcttgatca tggaaggtcg tgatattgac
    28801 ctttacgcag ggatgaatct tccaatggtg attgaattta tcaatgcgag ccttacaggc
    28861 gcagatgcgg actacaagag ccgtgctgca gaaagcattg tgaaagttaa tgacctgtta
    28921 gcgggcttcg atgatgacga agatgaataa tactcttcga aaatctcttc aaactacgtc
    28981 aacgtcgcct tgccgtaggt atatgttact gacttcgtca gtcttatccg gcaacctcaa
    29041 aacggtgttt tgagctgact tcgtcagtct tatccggcaa cctcaaagca gtgctttgag
    29101 cagcctgcgg ctagtttcct acagatttta gttggaactc gattcaattc atgtgacaac
    29161 gtgaaaatcg ttagagcatt ttatatagaa tatacatggg aatgtagctt actcccattc
    29221 ccatatttaa tagaaaaaga ggaactcaat gctacattat acaaaagaag acttgctcga
    29281 attgggtgca gaaatcacta cgcgtgaaat ctaccaacag cctgatgtat ggagagaagc
    29341 ttttgaattt tatcaagcaa aacgtgaaga aattgcagcc ttcctacaag aaatcgctga
    29401 taaacatgac tatattaagg ttatcttgac aggtgctggg acttctgctt atgtgggaga
    29461 taccttgcta ccttatttta aggaagtcta tgacgaacgc aaatggaatt tcaatgctat
    29521 tgcgacaaca gatatcgttg ccaatccagc aacctatttg aaaaaagatg tggcaactgt
    29581 ccttgtgtct tttgctcgta gtgggaattc gcctgaaagt ttggcgactg ttgatttggc
    29641 caaatccttg gtggatgagc tttatcaagt gacgattact tgtgcagcag atggtaaatt
    29701 ggctcttcaa gctcacggtg atgatcgtaa tctcttgctc ttgcaaccag ctgtctctaa
    29761 tgatgctgga tttgccatga cttctagctt tacgtctatg atgttgacaa ctctcttggt
    29821 ctttgatcct acagaatttg ctgttaagtc tgaacgtttt gaagttgtat ctagtcttgc
    29881 ccgtaaagtt ttagacaagg cagaagatgt caaagagctc gttgatttag actttaaccg
    29941 tgtcatctat ctaggcgctg gtcctttctt tggacttgct catgaagctc agctcaagat
    30001 tttggaatta actgctggtc aagttgcgac catgtatgaa agcccagttg gcttccgtca
    30061 cggtccaaaa tctcttatca acgacaatac agttgttttg gtctttggta caacgacaga
    30121 ctacactcgt aagtacgact tggacttggt tcgtgaagtt gctggtgacc agattgctcg
    30181 tcgtgttgtg cttttgagtg atcaagcttt tggtcttgaa aatgtcaaag aagtggccct
    30241 tggttgtggc ggtgtcttga atgatattta ccgtgtcttc ccttacatcg tttatgccca
    30301 actctttgct ttattgactt cactcaaggt agaaaataaa ccagatacac cgtctcctac
    30361 aggtacagta aaccgtgtag tacaaggtgt cataattcac gaatatcaaa agtaagacag
    30421 tgtttatgaa ttcttgacaa gaggatttgt aaattatcag ataaaccata gattgtcagt
    30481 acgctttcta tggtttgttt gcttgagaga aatagtaaaa ggagaacaga atgaaagcat
    30541 acacagagcg tgtatttgga aatgttgagg gtgaggatgt cttggcctat cgatttgaga
    30601 cagacggtgg ctaccaactt gaggttatga cttatggtgc gactatcttg cgctatgtcg
    30661 cacctgacaa ggctggaaat tttgccaatg ttatcttggg atttgatgac tttgatagtt
    30721 atgtaggcaa tagtcccaag catggagcaa gtgtaggtcc tgtagcgggt cgtattgcag
    30781 gtgcgacctt tgagctcaat ggtaagacct atgaccttga ggttaataat gctagcaact
    30841 gtaatcacag tggttcaact ggttgggatt ccagcttgtt tgaagttgaa gaagtaagcg
    30901 atcatggctt gactctctac acagagcgta cagatgggac aggagggttc cctggaaatc
    30961 tcaagatttg gatcagttat cacttggaag aaactggtgc ctatgaaatc agctacaagg
    31021 taacgaccga tcaggatacg ctggtcaatc caaccaacca cagctatttc aacttgtctg
    31081 gtgatttcac gcagacgatt gaccgtcatg tcttccaact aaacacagag ggcatttact
    31141 caatcgctcc tgacggtgtt cctgccaaaa ctccagaagc caaccgtgat gtggtcaaac
    31201 acgtctacaa tggtaccttg ttgaaggata tctttgcaga agaagatgag caaatccagc
    31261 tggcatcagg tttggatcat ccatttgccc ttcctgcagg ccatgacaat gctggattcc
    31321 tttatgacca aaattcaggt cgcttcctgc ttttcaagac agaagctcct tgctttgtgg
    31381 tctacacagc aaactttgtg gatgaaagtg tcatcatagg aggtcagcca atgctacagc
    31441 acaatgggat tgctcttgaa gcgcaagctt taccagatgc cattcacagt gaccttaaag
    31501 gccaagtcat tcttaaagct ggtcaaacct tcaccagtaa gacacgttat gaacttgttg
    31561 tgaaataaaa aaagctggtc aaaaagtctt taaaattaaa ataacgcata ttgtcaggtg
    31621 atgaaaagtt tgataatatg cgttttatta tgaaatgatt tactttatat tatcctgaaa
    31681 tggtgttatt tttgagttat ctggcttgac tttcttgatg gaaattatat aatagttgta
    31741 acaataataa ttacaactag aaaagaggac catttatgac ctatgaatac aagagtcaca
    31801 tttatttggc agagacagct ttaaatgtaa aggatttggc cagtcaaaca gccttttatc
    31861 agcaagttat tggtttagaa atcttatctc aaactgagac agagtccatt ctaggccttg
    31921 gtggaaaagt cttggtacag ctgattcaag cacaagagag cggagaagtg agggaatatt
    31981 atggtcttta ccatttggcc attctcttgc ccacacgcaa ggctttagct gatgtcttga
    32041 aacacctgac agatttacaa attcctcttg tcggcggtgc agaccatggt tacagtgaag
    32101 ctatttactt ggaagatttg gagggaaatg gcattgaact ctatcgagat aagccagttt
    32161 ccacatggga tattcgagaa gatggacgca ttatcggagt gacggaggct cttgcggcgc
    32221 aggacattta tgagttgggg gaaagagtag agccctttat cctggcagaa ggtacaagaa
    32281 tggggcatat tcatctttcc gtcaaggata gtcgaaagtc cagccagttt tatcaaaagg
    32341 tgttagggct agagaataaa ttcagtgtgc ctggcgctag ttggattgcg gctggagatt
    32401 accatcatca tttagcagtt aacgaatggg gaggaaaagg tctggcttcg cgtaagcaag
    32461 gcttaccagg tttagcctac tatgtcatcg aagtcgcaca taaaaaagaa ctgttaacga
    32521 ttgcccaacg agcacaagaa gttgacgcac caatcaaatg gatgacatcg atccaattgg
    32581 aaatcacaga ctcagatggc atcgtgaccc gtattcgttt agcaaggtaa aattaggaat
    32641 ttttaataag ttataagtat tgctagtttg ctttttgaac ggtctttggt aacagaactg
    32701 cgtgagaatt ttgacaaaga agctgattag agatataatg aagaaaaatc gagcaaggaa
    32761 aaagaactga caaattcaaa gtatattact tgtttgaaac gttcagaggg ccagttgtgt
    32821 gggattcaaa agatgattga aggagatcgt gactgtgctg atattgtgac gcaactgaca
    32881 gcagtgaaat ctagtgtaga gcgcgtgatt gagatgataa ttaccgaaaa tcttactgaa
    32941 tgtatcaatc agcccctaga tgattctgaa gctcaaaagg aacgcctaga aaaggctatc
    33001 cgatacttga ttaaacggaa ataaatgaat gttttgagac gatatgtgca aaggtctatt
    33061 aactattaga atagtttaat gtgagtcaaa agaagttgta aaaaatctct aaaattaaaa
    33121 aaacgcatag tatcaggtgt tgaatgtact gacccccaaa agttagattt tttctgtcta
    33181 acttttgggg tgcagttcat aagaaccttg gtaatatgcg ttttattatg tagagactta
    33241 tactcttcga aaatctcttc aaaccacgtc agcttcacct tgccgtagat atggttactg
    33301 acttcgtcag ttctatccac aacctcaaaa cagtgttttg agctgacttc gtcagttcca
    33361 tccacaacct taaaacagtg ttttgagcag cctgcggcta acttcctagt ttgcactttg
    33421 attttcattg agtattactt catcttttcc tgaaattgag tttttgccca actcctcttg
    33481 attattctac ttgaccgggc caagcattca taccaccttc tacgttggta acggtgaggc
    33541 cttgggagct gagaaactgg caggtagatg cagagcggac tcctccttga caaatgacat
    33601 agtattcatg gtcaggtttg agttctttgt agccttgctc taaggtactt aatggaagat
    33661 ttttggcacc tggtgcatgt cctgcttgga attcatgtgc ttcacggaca tcgataagtt
    33721 ctagattttc attttggtat tttccataaa agtcagccat gctgatacta gtttccatat
    33781 tctactcctt ctgggttaac cattttgtat agtgaataag cgccgtcaag gttttgggcg
    33841 gtaaatcctg cttgcttgag gataggctct gcgatatagc tgcgcaaacc actgtggcag
    33901 ctgacgatgt aggcttgttt tttgtccagt tcatcagttt cctccttgac actttgtcag
    33961 tttttgatac aatagtacaa aattagagga ggcaggctat gattcagaaa catgcgattc
    34021 ctattttaga gtttgatgac aatcctcagg cggttatcat gcccaatcac gaggggctgg
    34081 acttgcagtt gccaaagaag tgtgtttatg catttttagg tgaggagatt gaccgctatg
    34141 caagggaagt aggggcggat tgtgtcggcg aattcgtttc tgctaccaag acctatccag
    34201 tctatgtcat caactacaag ggtgaggagg tctgtctggc tcaggctcct gttggctccg
    34261 ctccagcagc ccagtttatg gattggttga ttggctatgg tgtggagcag attatctcta
    34321 ctgggacctg tggtgtccta gctgatatag aggaaaatgc ctttctagtc cctgttcgcg
    34381 ctttgcgaga tgagggagcc agttaccact atgtggcacc ttgtcgttat atggaaatgc
    34441 agccagaggc tattgctgct attgaggaag ttttggaaga cagagggatt ccttatgaag
    34501 aagtcatgac ctggacgaca gacggttttt accgagaaac ggctgaaaag gtggcttatc
    34561 gtaaggaaga aggctgtgct gttgtggaga tggagtgttc tgctcttgcg gcagtagctc
    34621 aattgcgtgg ggttctctgg ggtgaattgt tgttcacagc agattctcta gcggacttgg
    34681 accagtacga cagtcgtgac tggggctcgg aagcttttaa taaggcgcta gaactgagtt
    34741 tagcaagtgt tcaccacctt tagttgtact ggcaaaggat ttgttttatc ataaaatgtc
    34801 tagctcatac ttttcaaaaa tatgtttaaa cgaggtcacc ttcctcttgt cctaggcatg
    34861 ttgaggttgg gaaaaatctt taaaatcaga aaaacgtatc atatcaggtg atgaaaattt
    34921 tgatactatg cgttttatgt tggtaagatt tagagtgaga tgaaatgata ctcttcgaaa
    34981 atctcttcaa accaggtcag cttcaccttg ccgtatagat gttactgact tcgtcagttc
    35041 tatctgcaac ctcaaaacag tgttttgagc tgacttcgtc agttctatcc acaacctcaa
    35101 aacagtgttt tgagcaacct gtgactagct ttctaagcga tgccttggtt ctcattgcct
    35161 ataatcaaaa agagaaattt tctcctgaaa agcatataga gtagctggcg ttaaaagctc
    35221 ctgtcttgct tttttgacct atagtcacat ctatcaagta ttgttcttgc ctaagctatc
    35281 aataaaaagg tggcattttt taggcttggt gttagtgaat tttgccttat cctatctaag
    35341 tcatttcgag ctttttatgg tacaatggaa acatgttatt caaattatct aaggaaaaaa
    35401 tagagctagg cttatctcgt ttatcgccag cccgtcgtat ttttttgagt tttgccttgg
    35461 tcattttact aggctctctt cttttgagct tgccctttgt ccaagttgaa agctcacgag
    35521 cgacttattt tgatcatctt ttcactgctg tctctgcagt ctgtgtgaca ggtctctcaa
    35581 cccttccagt agctcacacc tataatatat ggggccaaat aatctgtttg ctcttgattc
    35641 agatcggtgg tctagggctc atgaccttta ttggggtttt ctatatccag agcaagcaaa
    35701 agcttagtct tcgtagccgt gcaactattc aggatagttt tagttatgga gaaactcgat
    35761 ctttgagaaa gtttgtctat tctatttttc tcacgacctt tttggttgag agcttgggag
    35821 ctattttgct tagttttcgc cttattcctc aacttggctg gggacgtggt ctttttagtt
    35881 ccatttttct agcgatctca gccttctgta atgccggttt tgataattta gggagcacca
    35941 gtttatttgc ttttcagacc gatttactgg tcaatctggt gattgcaggc ttgattatta
    36001 caggcggcct tggttttatg gtctggtttg atttggctgg tcatgtagga agaaagaaaa
    36061 aaggacgtct gcactttcat acgaagcttg tactattgtt gactataggt ttgttgttat
    36121 ttggaacggc aactactctc tttcttgagt ggaacaatgc tggaacgatt ggcaatctcc
    36181 ctgttgccga taaggtttta gttagctttt ttcaaacagt gacgatgcga acagctggct
    36241 tttctacgat agattatact caggctcatc ctgtgactct tttgatttat atcttacaga
    36301 tgtttctagg tggggcacct ggaggaacag ctgggggact caagattacg acattttttg
    36361 tcctcttggt ctttgcacga agtgagcttc taggcttgcc tcatgccaat gttgcgagac
    36421 gaacgattgc gccgcgaacg gttcaaaaat cctttagtgt ctttattatc tttttgatga
    36481 gcttcttgat aggattgatt ctgctaggga taacagccaa aggcaatcct ccctttatcc
    36541 acctcatatt tgaaaccatt tcagctctta gtacagttgg tgtaacggca aatctgactc
    36601 ctgaccttgg gaaattggct ctcagtgtta tcatgccact tatgtttatg ggacgaattg
    36661 gtcccttgac cttgtttgtt agcttggcag attaccatcc agaaaagaaa gatatgattc
    36721 actatatgaa agcagatatt agtattggtt aagaaaggaa agagcatgtc agatcgtacg
    36781 attggaattt tgggcttggg aatttttggg agcagtgtcc tagctgccct agccaagcag
    36841 gatatgaata ttatcgctat tgatgaccac gcagagcgca tcaatcagtt tgagccagtt
    36901 ttggcgcgtg gagtgattgg tgacatcaca gatgaagaat tattgagatc agcagggatt
    36961 gatacctgcg ataccgttgt agtcgcgaca ggtgaaaatc tggagtcgag tgtgcttgcg
    37021 gttatgcact gtaagagttt gggggtaccg actgttattg ctaaggtcaa aagtcagacc
    37081 gctaagaaag tgctagaaaa gattggagct gactcggtta tctcgccaga gtatgaaatg
    37141 ggacagtctc tagcacagac cattcttttc cataatagtg ttgatgtctt tcagttggat
    37201 aaaaatgtgt ctatcgtgga gatgaaaatt cctcagtctt gggcaggtca aagtctgagt
    37261 aaattagacc tccgtggcaa atacaatctg aatattttgg gtttccgaga gcaggaaaat
    37321 tccccattgg atgttgaatt tggaccagat gacctcttga aagcagatac ctatattttg
    37381 gcagtcatca acaaccagta tttggatacc ctagtagcat tgaattcgta aagagggatg
    37441 acccctcttt tttgatgccc aagatggcaa atagagacag aagccccttg tcttctagta
    37501 aaagttcttc aaaggctgga ctttatggta aaatagaagg aagtgacaag agagagtaat
    37561 actcaatgaa aatcaaagat caaactagga aactagctgc gggctgctca aaacactgtt
    37621 ttgaggttgt ggatagaact gacgaagtca gctcaaaaca ctgttttgag gttgcagata
    37681 gaactgacga agtcagtaac atctatacgg caaggcgacg ttgacgcggt ttgaagagat
    37741 tttcgaagag tataagaaaa aatcagttcc ctaaaggagc agattatgaa gttattgtct
    37801 atcgctattt ctagctataa tgcagcagcc tatcttcatt actgtgtgga gtcgctagtg
    37861 attggtggtg agcaagttgg gattttgatt atcaatgacg ggtctcagga ccagactcag
    37921 gaaatcgctg agtgtttagc tagcaagtat cctaatatcg ttagagccat ctatcaggaa
    37981 aataaaggac atggcggtgt ggtcaatcgc ggcttggcag aggcttctgg gcgctatttt
    38041 aaagtagttg acagtgatga ctggtggatc ctcgtgccta cttgaaaatt cttgaaacct
    38101 tgcaggaact tgagagcaag ggtcaagaga tggatgtctt tgtgaccaat tttgtctatg
    38161 aaaaggaagg tcagtctcgt aagaagagta tgagttacga gtcagtcttg cctgttcggc
    38221 agatttttgg ctgggaccag gtcggaaatt tctccaaagg ccagtatatc atgatgcact
    38281 cgctgattta tcggacagct ttgttgcgtg ctagccagtt ctaactgcct gaacatactt
    38341 tttatgtcga taatctcttt gtctttacgc cccttcagca ggtcaagacc atgtactatc
    38401 tgcctgtcga tttctatcgt tatttgattg ggcgtgagga ccagtctgtc aatgagcaag
    38461 tgatgattaa gtgcattgac cagcaactca aggtcaatcg actcttggta gaccagcttg
    38521 atttgtccca agtgagtcat cccaaaatgc gagaatatct gctgaatcat attgaaatca
    38581 cgacggtgat ttccagtacc ctgctcaacc gatctgaaac agcggagcat ctggcaaaaa
    38641 aacgccaatt gtggacctat attcagcaga aaaatccaga agtctttcag gctattcgta
    38701 agaccatgtt gagccgtttg accaaacatt ctgtcttgcc agatcgcaaa ctgtccaatg
    38761 tcgtctatca aatcaccaaa tctgtttatg gatttaatta atataagtgt tttataagag
    38821 ggatttaaga aaaattttaa ctttttctta gtccttttta atttcaggag attatactag
    38881 agtcatcaaa taaagaaaga ctctaaggag aatcctatga aattcaatcc aaatcaaaga
    38941 tatactcgtt ggtctattcg ccgtctcagt gtcggtgttg cctcagttgt tgtggctagt
    39001 ggcttctttg tcctagttgg tcagccaagt tctgtacgtg ccgatgtggt caatccgacc
    39061 ccaggtcaag tcttacctga agagacatcg ggaacgaaag agggtgactt atcagaaaaa
    39121 ccaggagaca ccgttctcac tcaagcgaaa cctgagggcg ttactggaaa tacgaattca
    39181 cttccgacac ctacagaaag aactgaagtg agcgaggaaa caaactcttc tagtctggat
    39241 acactttttg aaaaagatga agaagctcaa aaaaatccag agctaacaga tgtcttaaaa
    39301 gaaactgtag atacagctga tgtggatggg acacaagcaa gtctagcaga aactactcct
    39361 gaacaagtaa aaggtggagt gaaagaaaat acaaaagaca gcatcgatgt tcctgctgct
    39421 tatcttgaaa aagctgaagg gaaaggtcct ttcactgccg gtgtaaacca agtaattcct
    39481 tatgaactat tcgctggtga tggtatgtta actcgtctat tactaaaagc ttcggataat
    39541 gctccttggt ctgacaatgg tactgctaaa aatcctgctt tacctcctct tgaaggatta
    39601 acaaaaggga aatacttcta tgaagtagac ttaaatggca atactgttgg taaacaaggt
    39661 caagctttaa ttgatcaact tcgcgctaat ggtactcaaa cttataaagc tactgttaaa
    39721 gtttacggaa ataaagacgg taaagctgac ttgactaatc tagttgctac taaaaatgta
    39781 gacatcaaca tcaatggatt agttgctaaa gaaactgtcg aaaaagctgt taaagataac
    39841 gttaa
//
LOCUS       DAVKLX010000014        39086 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKLX010000014 DAVKLX010000000
VERSION     DAVKLX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.014"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            148..282
                     /gene="rpmH"
                     /locus_tag="VW325_000802"
     CDS             148..282
                     /gene="rpmH"
                     /locus_tag="VW325_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9734237.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            566..1459
                     /locus_tag="VW325_000803"
     CDS             566..1459
                     /locus_tag="VW325_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9734238.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVGGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATLEY
                     KLETIPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            1764..2537
                     /locus_tag="VW325_000804"
     CDS             1764..2537
                     /locus_tag="VW325_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9734239.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            2537..3097
                     /gene="rnmV"
                     /locus_tag="VW325_000805"
     CDS             2537..3097
                     /gene="rnmV"
                     /locus_tag="VW325_000805"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9734240.1"
                     /translation="MKEKISQVIVVEGRYDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            3169..3990
                     /locus_tag="VW325_000806"
     CDS             3169..3990
                     /locus_tag="VW325_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9734241.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLDTS
                     RTRRPEYAELILEEHMSQIIEKEVYSTNDMLLIRLFFYQMLIRKDLAKFINQIEKLML
                     FLLEQKKVTKLENFFIIRDTLISGMCCLEKVGVTDCFNDYLSCLQEIMDKTQDYQKKP
                     LVFMFLWKQALRVERDFSLAESFYQSSKTFAKLIGDGFLVKKLTEEWQEDVKKYL"
     gene            4310..4606
                     /locus_tag="VW325_000807"
     CDS             4310..4606
                     /locus_tag="VW325_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9734242.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            4683..6713
                     /locus_tag="VW325_000808"
     CDS             4683..6713
                     /locus_tag="VW325_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9734243.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     LGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            6715..7356
                     /locus_tag="VW325_000809"
     CDS             6715..7356
                     /locus_tag="VW325_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734244.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            7349..7642
                     /locus_tag="VW325_000810"
     CDS             7349..7642
                     /locus_tag="VW325_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734245.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            7893..8765
                     /gene="rsmA"
                     /locus_tag="VW325_000811"
     CDS             7893..8765
                     /gene="rsmA"
                     /locus_tag="VW325_000811"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9734246.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLMPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            8767..9645
                     /gene="rsgA"
                     /locus_tag="VW325_000812"
     CDS             8767..9645
                     /gene="rsgA"
                     /locus_tag="VW325_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9734247.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            9656..10312
                     /locus_tag="VW325_000813"
     CDS             9656..10312
                     /locus_tag="VW325_000813"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9734248.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            10275..10937
                     /locus_tag="VW325_000814"
     CDS             10275..10937
                     /locus_tag="VW325_000814"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9734249.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSGYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            10939..12195
                     /locus_tag="VW325_000815"
     CDS             10939..12195
                     /locus_tag="VW325_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9734250.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            12185..13126
                     /locus_tag="VW325_000816"
     CDS             12185..13126
                     /locus_tag="VW325_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9734251.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            13393..14220
                     /gene="purR"
                     /locus_tag="VW325_000817"
     CDS             13393..14220
                     /gene="purR"
                     /locus_tag="VW325_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9734252.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            14287..15537
                     /locus_tag="VW325_000818"
     CDS             14287..15537
                     /locus_tag="VW325_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9734253.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            15663..16457
                     /gene="pflA"
                     /locus_tag="VW325_000819"
     CDS             15663..16457
                     /gene="pflA"
                     /locus_tag="VW325_000819"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9734254.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(16532..17458)
                     /gene="yidC"
                     /locus_tag="VW325_000820"
     CDS             complement(16532..17458)
                     /gene="yidC"
                     /locus_tag="VW325_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9734255.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(17536..17814)
                     /locus_tag="VW325_000821"
     CDS             complement(17536..17814)
                     /locus_tag="VW325_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9734256.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            17848..18588
                     /locus_tag="VW325_000822"
     CDS             17848..18588
                     /locus_tag="VW325_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9734257.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            18644..19327
                     /locus_tag="VW325_000823"
     CDS             18644..19327
                     /locus_tag="VW325_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9734258.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            19439..20431
                     /locus_tag="VW325_000824"
     CDS             19439..20431
                     /locus_tag="VW325_000824"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9734259.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            20496..21035
                     /gene="rsmD"
                     /locus_tag="VW325_000825"
     CDS             20496..21035
                     /gene="rsmD"
                     /locus_tag="VW325_000825"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9734260.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            21025..21513
                     /gene="coaD"
                     /locus_tag="VW325_000826"
     CDS             21025..21513
                     /gene="coaD"
                     /locus_tag="VW325_000826"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9734261.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            21497..22534
                     /locus_tag="VW325_000827"
     CDS             21497..22534
                     /locus_tag="VW325_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9734262.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHLDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            22592..22822
                     /locus_tag="VW325_000828"
     CDS             22592..22822
                     /locus_tag="VW325_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9734263.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            22846..24129
                     /gene="murA"
                     /locus_tag="VW325_000829"
     CDS             22846..24129
                     /gene="murA"
                     /locus_tag="VW325_000829"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9734264.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            24122..24310
                     /locus_tag="VW325_000830"
     CDS             24122..24310
                     /locus_tag="VW325_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9734265.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            24349..25173
                     /gene="endA"
                     /locus_tag="VW325_000831"
     CDS             24349..25173
                     /gene="endA"
                     /locus_tag="VW325_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9734266.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            25464..26795
                     /locus_tag="VW325_000832"
     CDS             25464..26795
                     /locus_tag="VW325_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9734267.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(26993..27063)
                     /locus_tag="VW325_000833"
     tRNA            complement(26993..27063)
                     /locus_tag="VW325_000833"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27029..27031),aa:Cys,seq:gca)
     gene            27479..31090
                     /gene="rpoB"
                     /locus_tag="VW325_000834"
     CDS             27479..31090
                     /gene="rpoB"
                     /locus_tag="VW325_000834"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9734268.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            31123..34800
                     /gene="rpoC"
                     /locus_tag="VW325_000835"
     CDS             31123..34800
                     /gene="rpoC"
                     /locus_tag="VW325_000835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9734269.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            34911..35324
                     /gene="ndk"
                     /locus_tag="VW325_000836"
     CDS             34911..35324
                     /gene="ndk"
                     /locus_tag="VW325_000836"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9734270.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIGQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(36253..36885)
                     /locus_tag="VW325_000837"
     CDS             complement(36253..36885)
                     /locus_tag="VW325_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734271.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(36887..38902)
                     /locus_tag="VW325_000838"
     CDS             complement(36887..38902)
                     /locus_tag="VW325_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9734272.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDSNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
BASE COUNT        11518 a         7551 c         8958 g        11059 t
ORIGIN      
        1 tagggcttgc attcgaaaaa gctggaaaca tttgcctaga gctattgaca aaagtgaaag
       61 aatcagatag aatagtaaag tatgtttagt aactgatcac tttatagccg gctaaacgaa
      121 tacaaaatct atgaggaggt attcatcgtg aaacgtactt atcaaccaag taaacttcgt
      181 cgtgcgcgta aacacggatt ccgtaaccgt atgtcaacta aaaatggtcg tcgcgtattg
      241 gcagctcgtc gtcgtaaagg acgcaaagtt ttggctgcat aatccaaacg aattctatca
      301 aaaatcagta ggaactcgag tctactgatt tttatttttg taaaaaagtt cagtagatgc
      361 aaatggattc ggaagcgatg ttatagtaga ttgaaactag aatagtacac ctctgtttct
      421 aaaacattgt tagaaatcga tttgactgtc ctgatcgatt tgtcctgtta ttattttatt
      481 ttactataaa gttgaagtag gtggagatgg tacagcaaca atcgtcttta aagatggttc
      541 agctattaca attccaggaa atcaattggt agcacaagat ccaaaagcac aagatagcac
      601 taaaccgact gctgaaaaat caactgttaa agcacctgct caaagagtag atgtaaaaga
      661 tataactcat ttaacagatg aagaaaaagt taaggttgct attttacaag caaatggttc
      721 agcattagac ggagcgacaa tcaatgtagg tggagatggt acagcaacaa tcacattccc
      781 agatggttca gtagtgacga ttctaggaaa agatacagtt caacaatctg cgaaaggtga
      841 atctgtaact caagaagcta cactagagta taagctagaa actataccag gtggagataa
      901 gggaggcaat actggaagct cagatgctaa tgcgaatgaa ggcggtggta gccaggcggg
      961 tggatcagct cacacaggtt cacaaaactc agctcaatca caagcttcta agcaattagc
     1021 tactgaaaaa gaatcagcta aaaatgccat tgaaaaagca gccaaggata agcaggatga
     1081 aatcaaaggc gcaccgcttt ctgataaaga aaaagcagaa cttttagcaa gagtggaagc
     1141 agaaaaacaa gcagctctca aagagattga aaatgcgaaa actatggaag atgtgaagga
     1201 agcagaaacg attggagtgc aagccattgc catggttaca gttcctaaga gaccagtggc
     1261 tcctaatgct gctcctaaga caacaagtgc accgcaagca actgcaggaa caatgcaaga
     1321 tgttacctac cagtcacctg ctggcaaaca attacctaac acaggttcag catcaagtgc
     1381 agcacttgct agtcttggtc tagtggtggc aacaagtggt tttgctttgc taggaagaaa
     1441 gactagacgt agaaaataga acagctagaa aattctattc tctacttaaa gttagattat
     1501 aagggggatt ttgagaagtc atcaatccta gtgatgggtg agaaaagtga gaacccaaga
     1561 taatcacata ctttagctga ataggaatat tctatcaatg tagccaatct cttctgtctc
     1621 taactgtgga ataggagatg ggcaatatcg gatagaaaag atagcagaat agctctctat
     1681 tgaagagagg aggggaaacc gaaaaattag gtgcccctcc tcttttttgg tataatagaa
     1741 gatagaaaac gaggttagaa gagatgattt ttgatacaca tacacacttg aatgtagaag
     1801 aatttgcagg tcgtgaggca gaagaaattg ccttggctgc tgagatgggt gtgatacaga
     1861 tgaatattgt tggttttgat aaaccgacga ttgagcgtgc cttggagttg gtagatgagt
     1921 atgagcagct ctatgcgact attggttggc atcctacaga agctggtact tatacagagg
     1981 aagttgaggc ttacttgttg gataagttaa aacattccaa ggttgtggct ttaggtgaaa
     2041 ttggcttaga ctaccattgg atgacagcgc ccaaagaggt gcaggagcag gtttttcgcc
     2101 gtcagattca gctatctaag gacttggatt tgccttttgt tgtccatacc cgtgatgcgc
     2161 tggaagatac ctatgagatt atcaagagtg agggcgttgg tcctcgtggt ggtatcatgc
     2221 attcattttc agggacgctt gagtgggcag agaagtttgt ggatcttggt atgaccattt
     2281 ccttctcagg agtggtgact ttcaagaagg caactgacct ccaagaagca gctaaagagt
     2341 tacctttgga caagatgttg gtggaaacag atgcgcctta cttagcacct gtacccaagc
     2401 gtggtcgtga aaataaaaca gcctatactc gctatgtggt cgactttatc gctgacttgc
     2461 gtggtatgac gacagaagag ctggcggtag caacgactgc aaatgcagaa cgaatttttg
     2521 gattggacag caagtaatga aagagaaaat ttctcaagtt atcgtggttg aaggtcgcta
     2581 tgatacggtc aatctcaagc gttatttcga tgtggagacc tatgagacac gaggttctgc
     2641 catcaatgat caggatatag agcgaattca gcgtctacac caacgtcatg gagtcattgt
     2701 ctttacagac ccagatttta atggggagcg gattcggcgc atgattatga cggccattcc
     2761 aacagttcag catgcctttc tcaagcgaga tgaagctgtt cccaagtcca agaccaaggg
     2821 gcgttctctg ggaattgagc atgccagcta tgaagacctg aaaacggctc tagctcaggt
     2881 gacagaacaa tttgaacatg agagtcagtt tgacattagt cgtagcgact tgattcgcct
     2941 tggttttcta gcaggggcag acagccgtaa gcgtagagaa tatctcggag agactctccg
     3001 aatcggctat tctaatggca agcaactcct caaacgccta gagttgtttg gggttacttt
     3061 ggcagaagtg gaagaagcta tgaaatctta tgagtaggaa agatgtagcc gttacaattt
     3121 tttaagtttc acagtatttt tcgaagcagg tagaagagga ggcgtctgat gttaattggt
     3181 caaaaaatta aagagattcg gatagaaaaa ggaattagtc gtccagattt ttgtggagat
     3241 gagcaagaac tgacagttcg tcaactgtcg cgaattgaaa gtggagcttc gcaaccgagt
     3301 ttgcccaagt tagcctatat tgctcgccgg ctaggagttc cagtttatag ccttatgccg
     3361 gatttttcag ctcttccttc tgcttattta gaattgaaat accagatttt acgtgaacca
     3421 atctatggta aagaagagga gtatgataag aaagaagcgt gtttggatac ttctagaact
     3481 agaaggcctg aatatgcaga gttaatactt gaggaacata tgtctcagat catagaaaaa
     3541 gaagtttatt caacaaatga tatgttgttg attcgtttgt ttttttatca aatgctcatt
     3601 agaaaagatc ttgccaaatt tataaatcaa atcgaaaagc taatgctctt tcttttggaa
     3661 cagaaaaagg taactaaatt agaaaacttc tttataatta gagatactct tatttcagga
     3721 atgtgttgtc ttgaaaaggt aggagtaact gattgtttta atgattatct atcgtgttta
     3781 caagaaatta tggataaaac tcaagattat caaaagaaac ctcttgtatt tatgtttttg
     3841 tggaagcaag cattaagagt agaaagagat tttagtttag ctgaatcatt ttatcagtct
     3901 tctaaaacat ttgcgaagct aattggagat ggatttctag taaagaaatt gacagaggaa
     3961 tggcaagagg atgtcaaaaa atatttataa acatagtgaa tcagtgacaa aaatgtcctt
     4021 gtcctcgtat caaaacagtt ctaaagttcg tctttagaac tgttttttta gatataagct
     4081 aaaaataata tgaaatagtt agattttaag gacattgatg tccttgatag tgtgataaag
     4141 atggagtata atgactataa aagttcattt cagggtgcta ttttgacaat ttagagaaaa
     4201 acaaataatt ttagaatatc atttggtaat ataatagtat caacgttgat aatggaaaga
     4261 ggagcaagta actcaacaat cttgtaatca aaaatagagg agtatcaata tgaaacaaac
     4321 agttaaaaag ttagctcttg tagcgagcat tgcagcaaca ttaggtggag gtgtatcagt
     4381 tgcctctgca gccgttcaat acccagaagg tggtgtttgg acttatggtt caggtaacgg
     4441 tggtgcttac tcaaactact atcacccttc aaaataccat agttcaacag ttgttagcag
     4501 aaaaactggt tcatctgaca agggatatgc tggtgctgga gggacttctc gtgcatggat
     4561 tcgtacttct tggggagaga aagttgcatt ctactataat gtttagaatg gtatagccct
     4621 attgtttagc tttacttggt caccttctca ctaagtgacc aagtaacttt ttggaggaaa
     4681 tgatgaaacg tttatttatt ttgatttcaa tggtattggt atcgctttat atggtgataa
     4741 cttccgttga ccatcgagaa gagattttat ttggtaacta tccttctgtt gatgtgacag
     4801 gaatgatgat aaatcaacca gtagctagtc gcgaagaggt gacagaggct ttgagtcact
     4861 tggcggtaga gcataatagt ctcatcgccc gtcgaatcgt tgagccaaat gaagctggag
     4921 aaacactctt tacctatgcc acttatggtg agggagagct tccagaaggt ctgaccattt
     4981 cctccaagga gagtgcagaa acgagtgatt tattagggtc ttacttgatt gtgtcaggaa
     5041 gtttggatgg agtgagctta cagaccacct tgaaagagct tggttatcaa ggctttgttt
     5101 cgaatggaga agatccattt tcgatagtct tactattggc ggccacccct atggtgctac
     5161 tgagtttagc tatttttctg ctgaccttta tgagtctgac cctgatttat cggatcaaat
     5221 cccttcgtca ggcagggatt cgcttaatag ctggtgagag cttgtttgga gttgctctca
     5281 gaccagtgtt agaagatgtg agacagctta tctgctcagt gctggtatcc agtcttttgg
     5341 gattggggat tctctggtat caaggtgcct tgtttatggc aacggtacaa ctggtcatca
     5401 ttgctcttct actttatgga ttgaccttgg cagggatttc taccttacta agtgtcgtct
     5461 atctacttgg tttacaggaa aatagtctgg tggatctatt gaaagggaaa ctccctctca
     5521 aacgtatgat gacattgatg atggtggggc aactcttagc tgtattggtg gtcggatcga
     5581 gtgcgacagc tctcctaccc cactaccgtg aaatgcagga aatggagaga gctagcaata
     5641 aatggagcca gtcctcagac cgttaccgtc tatcctttgg ttggtctagt gcatttgccg
     5701 atgaagaagg aacgcgtaag gataatcgtg agtggcagac atttactgaa gaacggttag
     5761 ccaatacaga ctctttttat attatgagca atgttgacaa tttctcagat ggagcagaag
     5821 tggacctaga tggcaatcgt ctcagtgact acacaccgtt agggaatgtt atctatgtct
     5881 cgccgcgcta tctgatagaa gaaaagatta ccgtttcttc agagtttatg gacaagatgc
     5941 aaaacttgtc tgagggagag tttgggctga tcttgcctga gagcttgcga gagcagtctg
     6001 cctactacca aggattgttt acagattacc tgcaaaactt ttcatctgaa agtgtagaag
     6061 tgacgagtca gaaacactac ctcccacagg taaggctagc ttttacagaa acaggacagg
     6121 aacgtttcct ctataatgat gggtacaaga caacacgcca gtacctaaaa gatccgatta
     6181 ttgtagttct aacgccgcaa gcgactggaa caagacctgt tgcagggatg ttgtggggaa
     6241 ctacggctaa tagtgccttg aaactagatc gatatggaga cagcatcaca gctctaaaag
     6301 agcaaggtct gtatcacaag gtttcttact tggtaaaaag ccagctattt tttgccaagg
     6361 tactaaatga caaacgggtg gagttttact ctctccttat tgggacgatt ttgaccctgt
     6421 ctacggctat cttgttattt gattccatga atcttctcta ttttgagcag ttcagacggg
     6481 aacttatgat taaacgtctt gctggtatga caatctatga gcttcatggc aagtatttac
     6541 tggcgcaagg aggagttctc ttgcttggcc tagtcctatc tagtattttg acaagagatg
     6601 gtttgattag cgctctagtt gtagctttgt ttacgcttaa cgccctcttg attttagtaa
     6661 ggcaggataa aaaagaagaa gctggtagca tggcagtatt gaaaggaaaa taagatgatt
     6721 gatattcaag gattggaaaa gaaatttaat gaccgcgcga ttttctctgg tttgaatctc
     6781 aagctggaga agggcaaggt ttatgcctta atcggaaaga gtggaagcgg aaagacgacg
     6841 ctgctgaata tcttgggaaa gctagaaaag atagatggtg gaagggttct ctatcagggg
     6901 aaagatttaa aaaccattcc tactcgtgag tattttcgag accagatggg ctatctcttt
     6961 caaaatttcg gcctcttaga aaaccaatca atcaaagaaa atttggattt gggttttgtt
     7021 ggtcagaaaa tctcaaaagt agaacgtttg gaaaggcaag tgggggcttt agaaaaagtt
     7081 aatctagggt atttggattt agaacaaaaa atttatactt tatctggggg agaggcccaa
     7141 cgagttgccc ttgctaagac tattttgaaa aatccaccct tgattttggc agatgaacca
     7201 acagcagctc ttgatcctga aaattcagag gaggttatga atctcttggt ggatttgaaa
     7261 gatgaaaatc gaattatcat cattgcgacc cataatcccc tagtctggaa taaggctgat
     7321 gaaatcattg atatgaggaa acttgctcat gtgtgaaaaa atccgtattc gcagggtatc
     7381 tgattatcct agtgccagag gtggtttaga agatatcctc atcatggaaa atatgaccaa
     7441 tcatctcctt ttggttcaaa tccgagtgca tggctatttg cttgattttg ctagtattga
     7501 agggcaaagg caaaagcatt atcgtttgaa aaatttacct cagacggttg aactgacagt
     7561 ggatgatgtg gaggaggatg tggatttgac cctacccgaa aatcgaagtt atcaagaagc
     7621 tgattttttt gatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgt
     7681 acttgagtac ggcaaggtga agctgacgtg gtttgaagag attttcgaag agtatgaacg
     7741 tatgtttcga gagaagcact aagtccctct ttaaagattt ccaagactat ctttcttcat
     7801 gaggaaagat agttttttgg tatgattttc attcccaaaa tacaagggga atgtgttaca
     7861 atagtagtaa cagataatag aaaagagaat agatgagaat tgcagattat agcgtgacca
     7921 aggcagtgtt ggagcgtcac ggttttacct ttaaaaagtc ctttgggcaa aatttcttga
     7981 cggataccaa tatccttcaa aaaattgtgg atacggctga aattgatgat caggtcaatg
     8041 tcatcgaaat cgggccaggt attggtgcct tgacagaatt tttggctgag cgtgcagccc
     8101 aagtcatggc ttttgagatt gaccaccgtt tgatgccaat tttggcagat actctgcgtg
     8161 attttgataa tgtgaccgta gttaacgaag atattctcaa ggttgatttg gcgcaacata
     8221 tccaaaattt taaaaatcct aacctgccaa tcaaggtagt ggctaatttg ccttactaca
     8281 tcacgacgcc tattctcatg cacttgattg agagtggcat tcctttttgt gagtttgtgg
     8341 tcatgatgca gaaagaagta gcggaccgca tttcagccca gcctaacacc aaggcttacg
     8401 gtagcttgtc tatcgccgtg cagtattaca tgacagccaa ggttgccttt atcgtgcctc
     8461 gtacggtctt tgtgccagcg ccaaatgtgg attcagccat cttgaaaatg gtgcgtcgtc
     8521 cagagccagc cgtagcagta gaagatgaga actttttctt taaggtttcc aaggctagtt
     8581 ttacccatcg ccgcaagacc ttgtggaata acttgacagg ttactttggt aagactgaag
     8641 aggtcaagga caagctgacc aaggctttgg accaggcagg cttgtcacca agtgtgcgtg
     8701 gggaagctct cagcttggca gaatttgccg gtctagcaga cgcacttaaa gggcaaggac
     8761 tctaagatgc agggacaaat cattaaagcc ttggcaggtt tctactatgt ggagagtgat
     8821 ggccaggttt atcaaacacg cgcgcgtggg aatttccgta aaaaaggcca taccccttat
     8881 gttggggact gggtagattt ctctgccgag gaaaattcag aaggctatat cctcaaaatt
     8941 cacgaacgga aaaacagtct ggttcgtccg cctattgtca atatcgatca agctgtagta
     9001 atcatgtccg tcaaggaacc tgattttaac agcaatttgc tggatcgttt cttggttctt
     9061 ttggagcaca agggcatcca tcccattgtc tatatttcca aaatggattt gttggaagat
     9121 aggggagaac tggattttta ccagcagacc tatggtgaca tcggctatga ctttgtgacc
     9181 agtaaagagg aactcctgtc tttgttaaca ggcaaggtta cggtctttat ggggcagaca
     9241 ggtgttggga agtcaactct tctcaataaa atcgcaccag acctcaatct tgaaacggga
     9301 gaaatttcag acagtctagg tcgcggtcgc cataccactc gagctgttag tttttacaat
     9361 ctcaacgggg gtaaaatcgc agatacacca ggcttttcat ctctggacta tgaagtatca
     9421 agggctgaag acctcaatca ggcctttcca gagattgcta ctgttagccg agattgtaag
     9481 ttccgtactt gtacccatac ccatgagccg tcttgtgccg tcaaaccagc tgttgaagag
     9541 ggtgttattg caaccttccg ttttgacaat tacctgcaat tccttagtga aattgaaaat
     9601 cgtagagaaa cctataaaaa agtcagcaaa aaaattccaa aataaggaga aacctatgtc
     9661 tcaatacaag attgctccgt caattctggc agcagattat gccaactttg aacgtgaaat
     9721 caaacgtcta gaagcaactg gggcagaata tgcccatatc gatatcatgg acagtcattt
     9781 tgtaccgcaa atcagttttg gtgcaggtgt ggtcgagagc cttcgtcctc atagtaagat
     9841 ggttttcgat tgccacttga tggtgtcaaa ccctgagcat catctggaag attttgcgcg
     9901 tgcaggtgca gacatcatca gtatccatgt agaagcaacg cctcatattc atggcgccct
     9961 ccaaaaaatt cgttcactcg gagttaagcc ttcagtcgtt atcaatcctg gcacatcagt
    10021 tgaagccatc aagcacgtcc ttcatctagt tgaccaagtt ttagtcatga cggttaatcc
    10081 aggttttggt gggcaagcct ttctgccaga aaccatggat aaggttcgtg agttggttgc
    10141 tcttcgtgag gaaaaaggtt tgaactttga aatcgaagtg gatggtggga ttgatgacca
    10201 aactattgcc caagccaaag aagctggtgc gaccgttttt gtagcaggtt cctatgtctt
    10261 taagggagat gtcaatgagc gagtacaaac tctcagaaaa caactggact agggttgccg
    10321 tttttgcagg cggagaccgc ggtcattatc ggacagattt tgatgctttt gttggggtgg
    10381 atcgaggctc gctctgggtc ttggaagaag acttgcttct tgctctagca gttggggatt
    10441 ttgattctgt gaccgaagaa gagcgacagg tgattcaaaa acgtgcccag cattttgtcc
    10501 aagcccgacc agaaaaagat gataccgatt tggaattggc tctcttaacc atctttgaga
    10561 aaaatcctca ggctcaggtt actattttcg gtgccttggg tggccgtatt gaccatatgt
    10621 tggccaatgt ctttctgcct agcaatccta agttggcacc ctatatgcat caaatagaaa
    10681 ttgaggatgg gcaaaacttg attacttatt gtccagaagg gaccagtcag ctagaacctc
    10741 gttcaggcta cgactatcta gcctttatgc cagttcggga tagccagctg actattcttg
    10801 gagccaagta tgagttgaca gaggaaaatt ttttctttaa aaaagtgtac gcttctaacg
    10861 aatatataga tagggaagtg tcggtaactt gcccagatgg ttatgtggtc gtactgcata
    10921 gcaaggacag gaggtaggat ggaaagttta cttattctat tattaattgc caacctagct
    10981 ggtctctttc tgatttggca aaggcaggat aggcaggaga aacacttaag taagagcttg
    11041 gaggatcagg cagatcattt gtcagaccag ttggattacc gctttgacca agccagacaa
    11101 gccagccagt tagaccaaaa agatttggaa gtggttgtca gcgaccgttt gcaagaagtg
    11161 cggattgaat tgcaccaagg tctgacccaa gtccgtcaag aaatgacaga taatctcctc
    11221 caaactagag acaagacaga ccaacgtctc caagccttgc aggaatcaaa tgagcaacgt
    11281 ttggaacaaa tgcgccagac ggtcgaggaa aaactagaaa agaccttgca gacacgctta
    11341 caggcttcct ttgagacagt ttctaaacaa ctggagtctg tcaatcgtgg ccttggagaa
    11401 atgcagacag ttgcccgtga tgtcggagct cttaacaagg ttctctctgg aaccaagacg
    11461 cgagggattc tgggagaatt gcaactgggg caaattattg aagacatcat gacacctgcc
    11521 cagtacgaac gagaatacgc aacggttgaa aactctagtg aacgagtgga gtatgccatc
    11581 aagttacccg gacaaggcga ccaagaatac gtctatctgc caattgactc taagtttcca
    11641 ctggcagatt attaccgctt ggaagaagcc tatgagacag gtgacaagga tgagattgaa
    11701 cgctgtcgta agtcactcct agcaagcgtc aagcgctttg ccaaggatat caagagcaag
    11761 tacatagcac cgcctcggac gaccaatttt ggagttttgt ttgttccgac agaaggtctt
    11821 tattcagaga ttgtgcgcaa tccggtcttc tttgatgatt tgagacggga agaacagatt
    11881 atcgttgcag gaccaagtac cctatcagcc cttcttaact ccctatcagt tggtttcaag
    11941 acccttaata tccaaaagag tgccgaccat atcagcaaga ctcttgccag tgtcaagacc
    12001 gagtttggca agtttggtgg tattctggtc aaggcacaaa aacatctcca acatgcctct
    12061 ggcaatattg atgaattatt aaaccgtcgt accatagcta tcgagcggac gctccgtcac
    12121 attgagttgt cagaaggcga gcctgcgctt gatctactcc attttcaaga aaatgaggaa
    12181 gaatatgaag attagtcaca tgaaaaaaga tgagctattt gaaggctttt acctaatcaa
    12241 atcagctgac ctgaggcaaa ctcgagctgg gaaaaactac ctagccttta ccttccaaga
    12301 tgatagtggc gagattgatg ggaagctctg ggatgcccaa cctcataaca ttgaggcctt
    12361 taccgcaggt aaggttgtcc acatgaaagg acgccgagaa gtttataaca atacccctca
    12421 agtcaatcaa attactctcc gcctgcctca agctggtgaa cccaatgacc cagccgattt
    12481 caaggtcaag tcaccagttg atgtcaagga aattcgtgac tacatgtcgc aaatgatttt
    12541 caaaattgaa aatcctgtct ggcaacggat tgtccgaaat ctctacacca agtatgataa
    12601 ggaattctac tcctatccag ctgccaagac caaccaccat gcctttgaaa cgggcttggc
    12661 ctatcatacg gcgaccatgg tgcgtttggc agacgctatt agcgaagttt atcctcagct
    12721 caataagagc ctgctctatg cggggattat gttgcatgac ttagctaagg tcatcgagtt
    12781 gacggggcca gaccagacag agtacacagt gcgaggtaat cttcttggac atatcgctct
    12841 cattgatagc gaaattacca agacagttat ggaactcggc atcgatgata ccaaggaaga
    12901 agtcgttttg cttcgtcatg tcatcctcag tcaccacggc ttgcttgagt atggaagccc
    12961 agtccgtcca cgcattatgg aagcagagat tatccatatg attgacaatc tggatgcaag
    13021 catgatgatg atgtcaacag ctcttgcttt ggtggataaa ggagagatga ccaataaaat
    13081 cttcgctatg gataatcgtt ccttctataa accagattta gattaataat ttaagaaaaa
    13141 tgagcatttt ttaggataag aatgttcgtt tttttatgtg aatatggtat aataagtaaa
    13201 agacaaaaat gaatactctt cgaaaatctc ttcaaactag ggtagtatcg ccttgtcgta
    13261 tgtatatatg caggtatatt acagggtttg tcagttctat tgacaatctc aaaacagtgt
    13321 tttgaaccac cagcgaccag ctttctagtt tgctttttga ttttttgaat aaaaatggaa
    13381 taggaaatag aaatgaaatt aagaagaagt gatcggatgg ttgtcatttc caactatttg
    13441 attaataatc cttataaact aactagtctc aatacttttg ctgaaaagta tgagtctgct
    13501 aaatcatcca tctcagaaga tatcgtcatt atcaaacgcg cctttgagga aattgaaatc
    13561 ggtcatatcc agacagtgac tggggctggc ggaggtgtca tcttcacacc gtctatttcg
    13621 agtcaggatg ctaaggaaat ggttgaagac ttgcgtacca agttgtcaga aagtgaccgt
    13681 atcttgccag gtggttatat ctatctgtct gatttgctta gcacaccagc catcttgaaa
    13741 aatattggtc gtattattgc caaaagcttt atggaccaaa aaattgacgc ggttatgacc
    13801 gtagcaacta agggtgtgcc acttgcaaat gcagttgcca atgtcctcaa tgtctctttt
    13861 gtcattgtgc gccgtgacct gaaaattacc gaaggttcaa ctgttagcgt caactatgtt
    13921 tcaggttcaa gtggtgaccg tatcgagaaa atgttccttt caaaacgtag tcttaaggca
    13981 ggtagccgtg tcttgattgt ggatgacttc ttgaaaggtg gcggaacggt caacggtatg
    14041 attagtctct tgcgcgagtt cgactcagaa ctggcaggtg tagcggtctt tgcggacaat
    14101 gcccaagaag aacgtgaaaa gcagtttgac tacaagtcac tcttgaaggt aactaatatt
    14161 gatgtcaaga accaagccat cgatgttgag gttggcaata tctttgacga agataaataa
    14221 gagatagaac taaaggttgg aacgattgtc ccagcctttc tttgcaaaca gaatagaagg
    14281 aagcttatga aaacaccatt tatcaataga gaagagttag aagcgattgt tgccgagttc
    14341 ccgactccct ttcacttgta tgatgagaag gggattcgtg agaaggcaag agccgtcaac
    14401 caagcttttt cgtggaacaa gggctttaag gaatattttg cagttaaggc tactccaact
    14461 ccagctattt tgaaaattct ccaagaagaa ggttgtggtg tggactgctc tagttatgta
    14521 gagcttttga tgagccataa actggacttt ctgggttctg agattatgtt ctcttccaac
    14581 aacacgccag acaaggaata cgcctatgca cgtgaattgg gtgcgaccat taacttggat
    14641 gcctttgaag atattgaaca tctggagaga gtagcaggca ttccagaaat catctcttgt
    14701 cgttataatc ctggaggcgt ttttgaactg gggacagaca ttatggacaa tcctggggag
    14761 gctaagtttg gcatgaccaa ggaccagctc tttgaagcct ttgctatctt gaaggaaaaa
    14821 ggagccaaga cttttgggat tcactccttc ctagcgtcca ataccgtgac ccatctctat
    14881 tatccagagt tggctcgtca gctctttgaa ctggctgttg aaatcaagga aaagttgggc
    14941 atttggctag actttatcaa tctttctggc ggtattggtg ttaattatcg tccagaccag
    15001 gagccgaacg atatcgcctt gattggtgag ggagttcgta aggtgtatga agaagttctt
    15061 acgtcagcag gtcttggtca ggtcaagatt ttcaccgaat tgggtcgttt tatgctggca
    15121 cctcacggtg ctctagtcac aagagtgact cataagaagg aaacctaccg tacctatcta
    15181 ggtgtggatg cctcagcagt caacctcatg cgtccagcta tgtacggagc ttaccatcat
    15241 attagcaacg tgacccatcc agatggacca gctgaagcgg tagatgtggt tggttcactc
    15301 tgtgaaaaca atgataaatt tgcagtcaat cgcgaactgc ctcatacaga aatcggtgat
    15361 ttgctggtaa ttcatgatac aggtgcccac ggattttcaa tgggctacca gtataatgcc
    15421 aaattacgtt ctgcggaaat cctctatacc gaagaaggta aagcccgtca aatccgccgt
    15481 gcagagcgcc ctgaggacta ttttgcaacc ttatatggct tcgattttga agaataatct
    15541 gataatagat tgaaaatgaa attgaaaaac agattgcttt ctaaaaaata ggcaaaaatc
    15601 ttgtttttcc ttcaagtcgt gatataataa aactataaaa cgttttcaag gaaggtaacg
    15661 atatgtctga agaaacaatt gattatggac aagtgacagg aatggtgcat tcgacagaaa
    15721 gctttgggtc agtagatggg cctggtattc gctttattgt ctttttgcag ggctgtcaca
    15781 tgcgttgcca gtattgccac aacccagaca cttgggctat ggagtccaat aagtcacgtg
    15841 aacggacggt agatgatgtc ttgacagagg ccttgcgcta ccgtggtttc tggggaaata
    15901 agggtgggat tacagtcagt ggaggagaag ctctcttgca gattgatttc ctgattgctc
    15961 tcttcaccaa ggctaaggaa caaggaatcc actgtacctt ggatacctgt gctcttcctt
    16021 tccgtaataa accacgttac cttgagaagt ttgacaaact catggctgtc actgacttgg
    16081 ttcttttgga tatcaaggaa atcaacgaag aacagcacaa gattgtcact agacaaacca
    16141 ataaaaatat cttggcttgt gcccagtatc tatcagatat tggaaaacct gtctggattc
    16201 gccacgtgct agttccagga ttgacagaca gagatgatga cttgattgaa cttggtaagt
    16261 tcgtcaagac cctcaaaaat gttgataagt ttgaaattct accttatcac accatgggtg
    16321 agttcaagtg gcgtgaactt ggaattccat attccctcga aggagtcaaa ccaccaacag
    16381 cagatcgcgt caagaacgct aaaaaactca tggataccga aagttatcaa gattatatga
    16441 aacgtgtaca tggatagaaa agaagcctga tggaaacatc gggcttttga tttgcaaaaa
    16501 gacttagcaa atcagctaag cctttttctt cttatctcga acgttgtttt ccagcgttgc
    16561 gatttttgtg ttttttcttg cttgtgatag cagttggttg ttcaggggta acgtcttttc
    16621 gtccacttgg tttagagaaa gcacttgctt ttggtgggtt cttggctagt tcttcacgga
    16681 cttttttgcg aagttttgga cgaacgatat agttgacgat aaactgttgg agaatcatca
    16741 tgaaaccacc gacaacccag taaagtgtga cactagctgg tgagaagagg gagaagacga
    16801 cgatcatgag tgggctcatg taaatcattt tcttgatttg ttctctttgc atttcatctt
    16861 ctactccgtg aagtgaaagg agcgattgaa gatagtaaag gacaccagca caggcaacca
    16921 aaatcatact tggagaacct agaggaatgc ctaggtagct tgcttgagca accccttcag
    16981 tatgttgggc agcaaagtag atagcagaga agaaaggcat ttgaaggagg atagggaaac
    17041 atcctacacc gccaaacatg ctgataccgt gctctttttg agcagcaaag agagcttgtt
    17101 gggcttcgag tttttcttct tgagtagtcg cttctttgag acgcgtttgg tgtggctcaa
    17161 ggacgtgctt gagggcgttc atcttttcag agtgaagcgt tgccttccat gattggtaga
    17221 taccaagtgg caagataatc aagcgcacga taatggttac gatgatgata gcgacaccaa
    17281 agcctagacc tttatcagta gcgaagtact tgatagcttc agccataggg gctccgatgg
    17341 tattccaaat aaatcctgtt ggctgacctg tggttttatc gacattgaca caaccagtca
    17401 agacaagcaa catagccgct cccatagctg agagtgcaaa acgtttaata gatttcactg
    17461 tttttattcc ttctttaaaa attatacctt tctattctac tgttttttta caaaatatac
    17521 aatagttctg gagacctaat ttgcgatttt aaagtcagag tagggaggaa agttgcttag
    17581 tttgacatct aagtagctta cttttgaaaa aggtgtcggt ccttttcgga tttcttggat
    17641 aaattttgcc atgatagcag atgagtctgc ttgggctaag atttccactg tgccatcgtc
    17701 gttattccat actcgacctg tgatgccacc aatttcaagt gccaagctgt aaacacccca
    17761 acgaaagccg actccctgta ccctaccttg ggcaatcatt ctaacctttt gcataccaaa
    17821 cccctttgat tgtgttataa tatttctatg actattataa cctcaaaagc caattctgtg
    17881 gtaaaaaatg ccaagaaatt acgccaaaaa aaataccgca agtctgccta cttgattgaa
    17941 ggctggcact tgtttgaaga agctgtccaa gctggagtga cgattgagaa aatctttgcc
    18001 ctagaaaatt atcgagatca gttagctact ttttcgcaaa ctgtctgggt gtcagaggat
    18061 attttgctag atttggcgga ttctcagact ccacagggaa ttgttgccgt ggttcaaaaa
    18121 gaagaagtag gacaagctga tttgagtcag ggcaagttct tgtttttgga agatgtgcaa
    18181 gatcctggta atgtaggaac tatcattcga actgcggatg cagcaggttt tacaggagtg
    18241 attgtttcag ataagtcggc agacatctac agtctcaaaa ccctacgttc catgcaaggt
    18301 agtcatttcc atctgcccat ttaccggatg actagtcaag cgcttcttga cgaaactaaa
    18361 aaggtagcta tcccagtgct agcaacaacc ctatctaaag attctgttga ttacagagaa
    18421 ctgccttcta tagaaaattt tgtactagtt atgggaaatg agggtcaagg aattagtccc
    18481 cttatggctg aaagtgcaga ccagttggtc catattagca tgaaggggca ggccgagagt
    18541 ttgaatgttg cggttgcagc cggtatttta atcttccatt taagctaatt ttaacttttt
    18601 ttgttataat caaggaaaga tgttcacaga aaaggagaaa tggatgaatc acactattat
    18661 acatgaccgt gcaggtctca atcaatttta cgctaaggtt tatgcctttg ttggtctggg
    18721 aatcggacta tctgctttgg tatcaggcct tatgttgacg gtctttcagt ctcagttggt
    18781 ttactttttg atgcaggggc gtctctggtt gaccattgct acttttgcgg agttggcttt
    18841 ggttttcgtt gccagtagca tggcttcaag aaatagtcca gctgctcttc cagtattttt
    18901 actttactcc gttttaaacg gctttaccct tagttttgtg gtggctttct atactccggg
    18961 tacagtttta tctgcctttg tatcgagcgc ccttctcttc tttgtcatgg cggcagttgg
    19021 tatttttacc aagaaagact tgagtgggat tggtcgagca atgatggcag ctctcattgg
    19081 tttactgatt gctatggtag tcaatatttt cttggctagt ggcttctttg attatatgat
    19141 tagcgtagcc atggttttgg ttttttcagg gttgattgct tgggataacc aaaagattcg
    19201 cctcgcctat gaacaatcac aaggtcgtgt agcgacaggc tgggtggtgt caatggctct
    19261 cagcatctat cttgatttta tcaatctttt cctaagtatt ctacgtatct ttggtcgcaa
    19321 tgactaaaag gtctatgaaa tcagtgtttt gagtgacact gatttttttt aaattttctc
    19381 ttttcttttc ttggaaatag gtgtataata cgtttattaa atttttgagg agttgtctat
    19441 gaagaaaagt tttatccatc aacaagaaga aatttccttt gtcaaaaaca cttttaccca
    19501 gtatttgaaa gataagctag aagttgtcga agttcaaggt cctatcttga gtaaggtcgg
    19561 tgacggaatg caggacaacc tgtctggtgt ggaaaatcca gtatcggtca aggttctcca
    19621 aatccctgat gctacttatg aagtggtgca ctcacttgct aaatggaaac gccacacctt
    19681 ggctcgtttt ggctttggtg aaggagaggg tctctttgtc cacatgaaag cccttcgtcc
    19741 agatgaggat tccttggatg caacccactc tgtttatgtt gaccagtggg attgggagaa
    19801 ggttatccca aatggtaagc gtaacatcgt ttatctaaaa gaaacagttg agaagattta
    19861 taaggctatt cgcctgactg agctagctgt tgaagcccgc tatgacatcg agtctatctt
    19921 gccaaaacaa atcaccttta tccatacaga agaattggta gaacgctacc cagacttgac
    19981 acctaaagaa cgtgaaaatg cgatttgtaa agaatttgga gccgtctttt tgattggtat
    20041 cggtggcgag ttgccagatg gtaaaccgca cgatggacgt gcaccagact atgatgactg
    20101 gacaagcgag tctgagaatg gctacaaggg tctaaatggt gatattcttg tctggaatga
    20161 gtctttaggt ggagcctttg agttgtcttc tatgggaatt cgtgtagatg aagaaactct
    20221 tagacgtcag gtggaaatta caggtgatga agaccgcttg gaattggaat ggcacaagtc
    20281 tttgttgaat ggcctattcc cattgacaat cggtggagga attggacaat ctcgtatggc
    20341 catgttccta cttcgcaaga gacacatcgg agaagtgcaa acaagtgttt ggcctcaaga
    20401 agtccgcgat acttacgaaa atattttgta gagaatcgaa ccgcaaggtt cggttttctt
    20461 tctctttttg tctataattt ggtataataa acagtatgaa aatcgtatca ggaatctatg
    20521 ggggacgtcc cctcaagaca ctagaaggca agacaacaag acctacttcg gataaggtta
    20581 ggggagccat ttttaacatg attggtccct actttgaagg gggacgagtc ttggaccttt
    20641 atgcaggtag tggtggttta tctatcgaag cagtatcgcg tggcatgtcc agtgctgttt
    20701 tggtggagcg agaccgtaag gctcagacca tcgtggctga aaatatccag atgaccaagg
    20761 aagttggaaa atttcaactc ctcaagatgg atgcagagag ggcattggaa caggtatctg
    20821 gggaatttga cctcgttttc ttagaccctc cctatgccaa ggaacaaatc gtagcagata
    20881 ttgaaaaaat ggctaagaga gagctttttt ctgaagatgt tatggttgtg tgcgagacgg
    20941 ataaggccgt tgaacttcca gaagagattg cctgtctggg tatctggaag gaaaagattt
    21001 atggaattag taaggtgaca gtctatgtca gataagattg gcttattcac aggctcattt
    21061 gatccgatga caaatgggca tctggatatc attgaacggg cgagcagact ttttgataag
    21121 ctttatgtgg gtattttttt taatccccac aaacaaggat ttctccctct tgaaaatcgt
    21181 aaacgggggc tagaaaaggc tttgggacat ttggaaaatg ttgaagtcgt ggcttctcat
    21241 gatgaattgg tggtcgatgt tgcaaaaaga ctgggggcta ctttcctagt acgggggttg
    21301 aggaatgcgg cggatttgca atatgaagct agttttgatt actacaatca tcagctgtct
    21361 tctgatatag agactattta tttacatagc cggcctgaac atctctatat cagttcatca
    21421 ggtgttcgag agcttttgaa gtttggtcag gatattgcct gccatgttcc cgagagtatt
    21481 ttggaggaaa taagaaatga aaaaaaagat tagatggccc ttatacgtca ttgcggcctt
    21541 gattgtgact ttcttggcat ttgtagtgcc cttgccttat tatatagagg ttccaggtgg
    21601 ttcggaagat attcgccaag tccttaaagt aaatgacaca gaagataagg aagctggtgc
    21661 ctatcaattc gttacggttg gtgttcaaca tgccacttta gctcatatga tttatgcttg
    21721 gttgacacct tttacagata ttcgtagtgc tcaggagact acaggtggtt cttccgatgt
    21781 tgaatttatg cgaatcaatc aattctacat gcaaacatcg caaaatatgg ccaagtatca
    21841 aggactaaaa acagctggta aggatatcga actcaagtac tttggagttt atgttttgaa
    21901 tgtgacggat aattcaacct ttaaagggat tctcaatatc tctgatacag tcacagcagt
    21961 caatgatcag acctttgata gttccaaaga cttgattgat tacgtcagtt ctcaaaaatt
    22021 aggggattcc gtcaaggtca cctatgaaga ggatgggcaa accaagtctg cagaaggaaa
    22081 gattatcacc ttggaaaatg gcaaaaatgg aattggaatc ggcttgattg accgtacaga
    22141 ggtaatcagc aatgtcccaa ttagcttttc aacagctggt attggcggcc caagtgctgg
    22201 tctcatgttt agtctagcta tctatactca aatagctcac ccagatcttc gtaatggtcg
    22261 tattgttgcc ggtacaggta ccattgaccg cgatggtaat gtgggagaca ttggaggtat
    22321 tgataagaag gttgtagctt cggctagggc aggtgctgct attttctttg ctcctgataa
    22381 ccctgttagc gaagaagaac aaaaggcgca tctggacgcg aaaaacaact accaaacagc
    22441 cctagaagcg gctaaaacaa tcaagacgga tatgaaaatc gtgcccgtta aaaccctaca
    22501 agatgcgatt gattacttga aaaacaatcc ctaatttttg agtgaagttt ccaaactagc
    22561 gcacaaacag agaagatggt ataatagtca aatggttcaa ttattattca ctctaagtag
    22621 tcatatgctc tttatttatg tgagttttta ccttttaaag gatcttgtta gatgggagaa
    22681 ggttttaaaa gtgacagatg ataatacaag aaaagttcgt ttattagtag ccttttttag
    22741 cattgtcata ggctacatcc tgagttcttt ctttattagc ctgtatcatt tgtggcaaga
    22801 agcgcttaga ggattattat aaaatcaaga gtaaaggaaa cgagtatgga taaaattgtg
    22861 gttcaaggtg gcgataatcg tctggtagga agcgtgacga tcgagggagc aaaaaatgca
    22921 gtcttaccct tgttggcagc gactattcta gcaagtgaag gaaagaccgt cttgcagaat
    22981 gttccgattt tgtcggatgt ctttattatg aatcaggtag ttggtggttt gaatgccaag
    23041 gttgactttg atgaggaagc tcatcttgtc aaggtggatg ctactggcga catcactgag
    23101 gaagcccctt acaagtatgt cagcaagatg cgcgcctcca tcgttgtatt agggccaatc
    23161 cttgcccgtg tgggtcatgc caaggtatcc atgccaggtg gttgtacgat tggtagccgt
    23221 cctattgatc ttcatttgaa aggtctggaa gctatggggg ttaagattag tcagacagct
    23281 ggttacatcg aagccaaggc agaacgcttg catggcgctc atatctatat ggactttcca
    23341 agtgttggtg caacgcagaa cttgatgatg gcagcgactc tggctgatgg ggtgacagtg
    23401 attgagaatg ctgcgcgtga gcctgagatt gttgacttag ccattctcct taatgaaatg
    23461 ggagccaagg tcaaaggtgc tggtacagag actataacca ttactggtgt tgagaaactt
    23521 catggtacga ctcacaatgt agtccaagac cgtatcgaag caggaacctt tatggtagct
    23581 gctgccatga ctggtggtga tgtcttgatt cgagacgctg tctgggagca caaccgtccc
    23641 ttgattgcca agttacttga aatgggtgtt gaagtaattg aagaagacga aggaattcgt
    23701 gttcgttctc aactagaaaa tctaaaagct gttcatgtga aaaccttgcc ccacccagga
    23761 tttccaacag atatgcaggc tcaatttaca gccttgatga cagttgcaaa aggcgaatca
    23821 accatggtgg agacagtttt cgaaaatcgt ttccaacacc tagaagagat gcgccgcatg
    23881 ggcttgcatt ctgagattat ccgtgataca gctcgtattg ttggtggaca gcctttgcag
    23941 ggagcagaag ttctttcaac tgaccttcgt gccagtgcag ccttgatttt gacaggtttg
    24001 gtagcacagg gagaaactgt ggtcggtaaa ttggttcact tggatagagg ttactacggt
    24061 ttccatgaga agttggcgca gctaggtgct aagattcagc ggattgaggc aagcgatgaa
    24121 gatgaataag aaatcaagct acgtaatcaa tcgtttactt ttagtcatca tagtactgat
    24181 tttaggtact ctggctctag gaatcggtct aatggtaggt tatggaatct tgggcaaggg
    24241 tcaagatcca tgggctatcc tgtctccagc aaaatggcag gaattgattc ataaatttac
    24301 aggaaattag gctggagaac cagccttttt ctaaagataa ggagaaatat gaacaaaaaa
    24361 acaagacaga cactaatcgg actgctagtg ttattgcttt tgtctacagg gagctattat
    24421 atcaagcaga tgcagtcggc acctaatagt cccaaaacca atcttagtca gaaaaaacaa
    24481 gcgtctgaag ctcctagtca agcattggca gagagtgtct taacagacgc agtcaagagt
    24541 caaataaagg ggagtctgga gtggaatggc tcaggtgctt ttatcgtcaa tggtaataaa
    24601 acaaatctag atgccaaggt ttcaagtaag ccctacgctg acaataaaac aaagacagtg
    24661 ggcaaggaaa ctgttccaac cgtagctaat gccctcttgt ctaaggccac tcgtcagtac
    24721 aagaatcgta aagaaactgg gaatggttca acttcttgga ctcctccagg ttggcatcag
    24781 gtcaagaatc taaagggctc ttatacccat gcagtcgata gaggtcattt gttaggctat
    24841 gccttaatcg gtggtttgga tggttttgat gcctcaacaa gcaatcctaa aaacattgct
    24901 gttcagacag cctgggcaaa tcaggcacaa gccgagtatt cgactggtca aaactactat
    24961 gaaagcaagg tgcgtaaagc cttggaccaa aacaagcgtg tccgttaccg tgtaaccctt
    25021 tactacgctt caaacgagga tttagttccc tcagcttcac agattgaagc caagtcttcg
    25081 gatggagaat tggaattcaa tgttctagtt cccaatgttc aaaagggact tcaactggat
    25141 taccgaactg gagaagtaac tgtaactcag taaaagataa gcctaaactc ctatgtcact
    25201 tatggatgta ggagttcttt ttactagttt aagcagggct agaataggtg ctaagaaaaa
    25261 atagtaactt ctaaaactaa cttccagttt cccacaacct agcttttagt atgagaaaaa
    25321 cgcgtgaaat cagtggaaat ccgtcttaga ctaacttcca gttttgggag agagatggaa
    25381 gttacgctaa gaaaaaaata atatgtattg gattaaattt tgtgatataa taaaggataa
    25441 gatactattt taggagaaga actatggaag acccaagtag tcagaatttg ttgctacaat
    25501 ttgttttatt gtttatcttg acggtattaa atgccttttt ctcagccaca gaaatggcca
    25561 tggtttctct taatcgtgcc cgagttgaac aaaaggcgga agaaggagac agacgctaca
    25621 tccgcctgct gaaggtacta gaaaatccta accacttttt atcaaccatt caggtaggaa
    25681 tcaccctgat tacaatctta tcaggggcga gtttaactga tactctagga cgtgtgattg
    25741 cctcttggct tgggaatggc gaaacagcac aagccgtggc aacttttcta tccttggcat
    25801 ttttgaccta tatttccatc gtttttgggg aattatatcc taagagaatc gctcttaatc
    25861 taaaggatgc cttggcaatt cgtacagcgc cgattatcat tgggatcgga aaactagtca
    25921 gtccttttgt ttggctttta gctgcttcga ccaatttctt gagccatttg actcctatgt
    25981 cattcgatga tgcggatgaa aagatgaccc gtgatgaaat tgcctatatg ctgacaaata
    26041 gtgaagaaac attggatgct gatgagattg agatgctaca aggtgtcttt tcgctcgatg
    26101 aactgatggc acgagaggtt atggtgcctc gaacggatgc ctttatggtg gatattcagg
    26161 atgatagtca agccattatc caaagtattt taaaacaaaa ttattctcgt atcccggttt
    26221 atgatgggga taaggacaat gtaattggaa tcattcacac caagagtctc cttaaggcag
    26281 gctttgtgga cggttttgac aatattgttt ggaagagaat tttacaagat ccactttttg
    26341 tacctgaaac tatttttgtg gatgacttgc taaaagaact gcgaaatacc caaagacaaa
    26401 tggcaatctt gctggatgaa tacggtggta tggctggttt ggtgactttg gaagacctct
    26461 tagaggaaat tgttggtgaa attgatgatg aaacggacaa agcagcaata gatgttcatc
    26521 agattggcga ggatacctac attgttcaag gaactatgac cctcaatgat ttcaataatt
    26581 actttgatgt tgaactggaa agtgatgacg ttgataccat cgctggttat tatttgacag
    26641 gagtgggaac tattccaacg actgagaaac tcagctatga attggtcagc caaaacaaac
    26701 agtttatctt aaccaatgat aaagtgaaga atggacgtgt taccaaggta aaagtccaaa
    26761 tcacagaagt cgaaatagaa gaagaaacag aataaactag aggcttttgt caccaggcgt
    26821 gacgaagctt ttttcaattg atttcaaaag ggaattatgc catggaaaga actctgccac
    26881 aaactcgctc caaaagtttt taaagttata cgtatttatt cgagggaaaa taagaaaagc
    26941 ccttcaaatt gggctttctg ttcatttgaa acctgataaa tcaagttata gaaggcggta
    27001 gacggatttg aaccgacgat caagcttttg cagagccgtg ccttaccact tggctatacc
    27061 gccttaacgt ttattattat accttgaaaa tgatttccag tcaatagttt tttcagtttt
    27121 ttgtaccata gcgtgattcg aatgtgatgc caagttttct atgggaaaaa atttaccaaa
    27181 aacctactga aaccccttga aaaatctagc taaatagggt ataatatggg taatcatttg
    27241 tcgtaggttt tgtctgaaat attgtccaga caaggctcac agcagttaaa tcttctgaaa
    27301 aagtcagatt tagctgctct ttttgtgctt tttttcaaga ttatgagcat ttgtaacaga
    27361 ggcttaaaga ttctgaaaat tcgtcaagag gacacggtga taaggggttt acaaccatat
    27421 gacgattaga aaagcctgat tgacaaggct tggaacttat ttacaaagga gaatcatctt
    27481 ggcaggacat gacgttcaat acgggaaaca tcgtacccgt cgtagttttt caagaatcaa
    27541 agaagttctt gacttaccaa atttgattga aattcaaact gactcattca aagctttcct
    27601 agaccacggt cttaaggaag tgtttgaaga tgtattgcca atttcaaact tcacagacac
    27661 aatggagttg gaatttgttg gatatgaaat caaggaacca aaatacacgc tagaagaagc
    27721 tcgtatccac gatgctagct actcagcacc aatttttgta accttccgtt tgatcaataa
    27781 agaaacaggc gaaatcaaga cccaagaagt tttctttggt gatttcccaa tcatgacaga
    27841 aatgggtact ttcatcatca atggtggtga acgtattatc gtttctcagt tggtccgctc
    27901 accaggtgtt tactttaacg acaaagtaga caaaaatggt aaggtgggct atggttcaac
    27961 tgttatccct aaccgtggag cttggttgga acttgaaagc gactcaaaag atatcaccta
    28021 cactcgtatc gaccgtactc gtaagattcc atttacaacc ttggttcgtg ctcttggttt
    28081 ctcaggtgat gatgaaatct ttgatatctt tggtgacagc gaattggttc gcaacactgt
    28141 tgaaaaagat atccacaaga atccaatgga ctctcgtaca gacgaagcct tgaaagaaat
    28201 ttacgaacgc cttcgtccag gtgagcctaa gacggctgaa agctcacgta gcttgcttgt
    28261 agctcgcttc tttgacccac gtcgttatga cttggcagca gttggtcgtt acaaaatcaa
    28321 taaaaaactc aatgttaaaa cacgtttgct caaccaaacc attgcagagc cattggtaga
    28381 ccctgaaact ggagaaatct tggtagaagc tggtacgatt atgactcgta gcgtgattga
    28441 aagcattgaa agccatttgg atggcgactt gaacaagatt gtctacatcc caaacgatgc
    28501 agccgttgtg actgagcctg ttgttcttca aaaattcaag gttattgctc caactgatcc
    28561 agatcgcgtc gtaacgatca ttggtaatgc taacccagat gacaaggttc gtacggtgac
    28621 tcctgcagat atccttgctg agatgagcta cttcctcaac ttggctgaag gacttggccg
    28681 tgtagatgat atcgaccacc ttggaaatcg tcgtatccgt gcggttggtg aattgcttgc
    28741 caaccaagta cgtttgggac tttctcgtat ggaacgtaat gtccgtgaac gtatgtctgt
    28801 tcaggacaat gaagtcttga caccacaaca aattatcaat attcgtcctg taacagctgc
    28861 agttaaagaa ttctttggtt catcacagtt gtcacagttc atggaccaac acaacccgct
    28921 ttctgagttg tctcacaaac gccgtttgtc agccttagga cctggtggtt tgactcgtga
    28981 ccgtgccgga tatgaagtgc gtgacgtgca ctacactcac tatggtcgta tgtgtccaat
    29041 cgagacacct gaaggaccta acatcggttt gatcaataac ttgtcatctt acggacactt
    29101 gaacaaatat ggttttgttc aaacaccata ccgtaaggtt gaccgtgaaa caggtgttgt
    29161 cacgaacgaa attgtttggt tgacagctga tgaagaagat gaatatactg tagctcaggc
    29221 taactctcgt ctgaatgaag atggaacctt tgctgagaag attgtcatgg gacgtcacca
    29281 aggggtcaac caagagtatc cagctaatat tgttgactac atggacgttt caccaaaaca
    29341 ggtagttgcc gttgcgacag catgtattcc tttcttggaa aacgatgact ccaaccgtgc
    29401 cctcatggga gccaatatgc aacgtcaggc tgtgccattg attaatcctc aggcacctta
    29461 cgttggtact ggtatggaat accaagcagc ccacgattct ggtgcggctg tgattgctca
    29521 gtatgatggt aaagttactt acgcagatgc tgacaaggta gaagttcgtc gtgaagatgg
    29581 ttcattggat gtttaccaca tccaaaaatt ccgtcgttca aactcaggta ctgcttacaa
    29641 ccaacgcact ctcgtaaaag ttggtgatgt cgttgaaaaa ggcgatttca tcgctgacgg
    29701 accttctatg gaaaatggag aaatggcgct tggacaaaac ccaatcgttg cctacatgac
    29761 ttgggaaggc tacaacttcg aggatgccgt tatcatgagc gaacgcttgg tgaaggacga
    29821 tgtctacaca tctgttcacc ttgaagaata cgaatcagaa acgcgcgata caaagcttgg
    29881 gcctgaagaa atcactcgcg aaattccaaa cgttggtgaa gatgccctca aagaccttga
    29941 cgaaatgggg attatccgta ttggtgctga ggttaaagaa ggtgatattc ttgtagggaa
    30001 agtaacacct aagggtgaga aagatctttc agctgaagaa cgtctcttgc acgctatctt
    30061 tggagacaag tctcgtgaag tgcgtgatac ttctcttcgt gtaccacacg gtgccgatgg
    30121 tgtcgttcgt gatgttaaga tctttacacg tgtaaatgga gatgagttgc aatcaggtgt
    30181 taacatgttg gttcgtgttt acatcgctca aaaacgtaag attaaggtcg gagataaaat
    30241 ggccggacgt cacggaaaca aaggggttgt ctctcgtatc gttcctgtag aagacatgcc
    30301 ttaccttcca gacggaactc cagtcgacat catgttgaac ccacttgggg tgccatcacg
    30361 tatgaatatc ggtcaggtta tggagcttca ccttggtatg gcagctcgta ctcttggtat
    30421 tcacattgcg acaccagtct ttgatggagc aagttctgaa gatctttggt caactgttaa
    30481 agaagcaggt atggatagcg atgccaaaac gatcctttac gatggacgta ctggtgaacc
    30541 atttgataac cgtgtttctg ttggagtcat gtacatgatc aaactccacc acatggttga
    30601 cgataaattg cacgcgcgtt cagtcggacc ttactcaact gttacccaac aaccactcgg
    30661 aggtaaagct cagtttggtg gacaacgttt cggtgagatg gaggtttggg ctcttgaagc
    30721 ctacggtgcg tcaaatgtcc ttcaagaaat cttgacttac aagtctgacg atatcaacgg
    30781 acgtttgaaa gcctatgaag ctattacaaa aggcaaacca attccaaaac caggtgttcc
    30841 agaatccttc cgagttcttg ttaaagaatt gcaatctctt ggtcttgaca tgcgtgtcct
    30901 agacgaagat gaccaagaag tggaacttcg cgacttggat gaaggaatgg acgaagatgt
    30961 catccacgta gatgaccttg aaaaagcccg cgaaaaagca gcccaagagg ctaaagcagc
    31021 ctttgaagct gaagaagctg agaaagcaac aaaagcggaa gcaacagaag aagctgctga
    31081 acaagaataa gcagttcact tagaatagaa agggaagaaa tagtggttga tgtaaatcgt
    31141 tttaaaagta tgcaaatcac cctagcttct ccaagtaaag tccgttcatg gtcttatgga
    31201 gaagtcaaaa aacctgaaac aatcaattat cgtaccttga aaccagaacg tgaaggactc
    31261 tttgatgaag tgatctttgg tcctacaaaa gactgggaat gtgcttgtgg taagtacaaa
    31321 cgcattcgtt acagaggaat tgtttgtgac cgctgtgggg ttgaagtaac gcgtacgaaa
    31381 gttcgtcgtg agcgtatggg acatatcgaa ttgaaagctc ctgtatctca catctggtac
    31441 ttcaagggga ttccaagccg tatgggcttg acccttgata tgagccctcg tgccctcgag
    31501 gaagttatct actttgcggc ttatgtggtg attgatccta aggatacacc acttgagcac
    31561 aagtctatca tgacagagcg cgaataccga gagcgcttgc gcgaatatgg ttatggttca
    31621 tttgttgcca agatgggtgc ggaagccatc caagaccttt tgaagcaagt agatcttgaa
    31681 aaagaaattg ctgaactcaa agaagaattg aaaacagcta ctggacaaaa acgtgtcaaa
    31741 gccatccgtc gtttggatgt tttggatgcc ttttacaagt ctggaaacaa acctgaatgg
    31801 atgattctta acatccttcc ggttatccca ccagatcttc gtccaatgtt gcagttggat
    31861 ggtggccgtt ttgcctcatc tgacttgaat gacctttacc gccgtgttat caatcgtaac
    31921 aaccgtttgg ctcgtttgct tgagttaaat gcaccaggta tcatcgttca aaatgagaaa
    31981 cgtatgcttc aagaagcagt tgacgctttg attgacaatg gtcgtcgtgg tcgtccaatc
    32041 acaggaccag gtagccgtcc attgaaatca ttgagccaca tgcttaaagg taaacaagga
    32101 cgtttccgtc aaaacttgct cggtaaacgt gttgacttct caggacgttc agttatcgcc
    32161 gttggtccaa ctcttaagat gtaccaatgt ggtgtgccac gtgaaatggc gattgaactc
    32221 tttaaaccat ttgtcatgcg tgaaatcgtt gcccgtgata tcgtgcaaaa cgtcaaagca
    32281 gctaaacgct tggtggaacg cggagatgag cgtatctggg atatccttga agaagtgatt
    32341 aaagaacacc cagtgctttt gaaccgcgca ccgacccttc accgtttggg tatccaagcc
    32401 ttcgagccag tcttgattga tggtaaggct cttcgcttgc acccacttgt ctgtgaagcc
    32461 tacaatgctg actttgacgg ggaccaaatg gccatccacg taccactttc agaagaagca
    32521 caagcagaag ctcgtatcct catgctagct gctgagcaca tcttgaaccc gaaagatggg
    32581 aaaccggtag ttactccatc tcaggacatg gttttgggta actactactt gaccatggaa
    32641 gaagctggtc gcgaaggtga aggaatggtc ttcaaagacc gtgacgaagc ggttatggct
    32701 taccgcaatg gttatgttca cctccactca cgtgttggta tcgcaacaga cagcctcaac
    32761 aagccttgga cagaagagca aagacataag gtcttgctta caacagttgg taaaattctc
    32821 ttcaacgata tcatgccaga ggggctacca tacttgcaag aaccaaacaa tgccaacttg
    32881 acagaaggtg ttccagctaa atacttcttg ccacttggtg gagatatcaa ggaagctatc
    32941 agcaatcttg agctcaaccc tccattcaag aagaaaaacc tcggaaatat catcgctgaa
    33001 atcttcaaac gtttccgtac gacagaaact tctgccctac ttgaccgcat gaagaacctc
    33061 ggttaccacc actcaactct tgcaggattg acagtgggta ttgccgatat cccagtcgtt
    33121 gatgacaagg ctgaaatcat tgaagaatca cacaaacgtg tcgaacaaat caccaaacaa
    33181 ttccgtcgtg gtatgatcac agacgacgag cgttacaatg ctgttacagc tgaatggcgt
    33241 gctgcccgtg aaaaacttga gaaacgcttg attgctaatc aagatcctaa gaacccaatc
    33301 gttatgatga tggactctgg agcccgtggt aacatctcaa acttctcaca gcttgccggt
    33361 atgcgtggtc tgatggctgc tccgaatgga cgtatcatgg aattgccaat cctttcaaac
    33421 ttccgcgaag gtttgtcggt attggaaatg ttcttctcaa ctcacggtgc tcgtaaaggt
    33481 atgaccgata cggcccttaa gacagccgac tcaggttact tgactcgtcg tttggtcgac
    33541 gttgcccaag acgttatcat ccgtgaggac gactgtggaa ctgaccgtgg tctcttgatc
    33601 cgttctatcg cagaaggaaa agagatgatc gagtctctcg aagagcgtct caacggtcgt
    33661 tacactaaga aaactgttaa acatccagaa actggtgcag tgattattgg tccaaatgaa
    33721 ttgattacag aagacaaggc gcgtgaaatt gtcaatgctg gtgtggaaga agtgactatc
    33781 cgttctgtat ttacatgtaa cactcgtcac ggtgtctgcc gtcactgtta cggtatcaac
    33841 ttggcgactg gtgatgcggt tgaagttggt gaagcagttg gtacaatcgc tgcccaatct
    33901 atcggggaac ctggtacaca gcttacaatg cgtaccttcc acacaggtgg ggttgcctca
    33961 aataccgata tcactcaggg tcttcctcgt gtccaagaaa tctttgaagc ccgcaatcct
    34021 aaaggggaag cggttatcac agaggttaaa ggacaagtta ctgctatcga agaggatgca
    34081 tcaactcgta ccaagaaagt ctttgttaag ggtgaaactg gcgaaggtga atatgtcgtt
    34141 ccatttacag ctcgtatgcg tgtcgaagtt gggggccaag tagcgcgtgg tgctgctctg
    34201 acagaaggtt ctatccaacc aaaacgtctc cttgcagttc gtgatgtctt gtcagttgaa
    34261 acgtaccttc tcggtgaagt acaaaaagtt taccgtagcc aaggggtaga aatcggtgac
    34321 aaacacatcg aggtaatggt tcgtcaaatg atccgtaaag tccgtgtcat ggatccaggt
    34381 gatacagatc ttctcatggg taccctcatg gatatcaatg actttacaga tgccaacaaa
    34441 gatgtcctta tcgcaggtgg agttccagcg acaggtcgcc cagtccttat gggaattacc
    34501 aaagcctcac ttgaaacaaa cagtttcttg tcagcggctt ccttccagga aacaactcgt
    34561 gtccttactg acgcagctat ccgtggtaag aaagaccatc tccttggact taaagaaaat
    34621 gttatcatcg gtaagatcat cccagctggt actggtatgg cccgttaccg taaccttgaa
    34681 ccacatgctg tcaacgaaga agaatacctt aatcctccag tagaggaaga aggaaatgaa
    34741 gaaacaacag aagtagttgt ggatactgcc gttgaaactg tggaagaaac agtagaataa
    34801 gagagaatga gagaaccctc gggttctctc attctctctt ctgaaaactt tctccatttt
    34861 tccacgaaat gtggtaaaat aaaagaaaga agctgacaaa ggagacgggt atggaacaaa
    34921 cattctttat catcaaacca gatggtgtaa aaagagggct agtgggtgaa gtgttaaagc
    34981 gcatcgaaca acgtggattt acaatcgaaa aattggagtt tcgttcacag gtttcagaag
    35041 agttgattgg ccagcactat caggacttgg ttggtcagag tttttaccca ccgattcgtg
    35101 aattcatgac ttcaggtcca gttcttgtgg gtgtcatttc tggtcccaaa gtaatcgaaa
    35161 cttggcggac catgatgggt gcaactcgtc cagaagaagc tttaccaggc actattcgag
    35221 gtgattttgc aaaagctgct ggagaaaatg agattatcca aaatgttgta catggttcag
    35281 attcagaaga gtcagctaag cgagaaattg ctctttggtt ttaagagtgg attggctcaa
    35341 tcaattggtt aaaagctcat ttgaatagaa agtatagtca attagtttaa gacatgacgc
    35401 atgatatcaa actttttagt ttttgatatg gtgcgttttt tgattaagtg actattagtt
    35461 tgtctcgctc cgttaggtgc gagacaaaaa aaacacccgc tatgcgggtg cgcgtcgaag
    35521 gttataccaa aaaaactcca aacgcgatac aataaaggtg ttcaatccaa ttgtaaagcg
    35581 aaaggagaaa aatatggcac aaaaggttct tttatcctgt tcttgtttca attgactata
    35641 tttctatact ttttcttcct ttctctgtga aaatcgcaag tttttcccac cttttctcaa
    35701 tactgaaaca acatctttcc agaacttcaa taatttcttt ttgtgttaaa tcattattta
    35761 actaaatctt tggaggcaat acgttttcaa agctactttt ttaccatagg ttttgagcct
    35821 tcaacacaga ttactaagcc aaaaatgctc taaatttttg aaattctaac aaattccact
    35881 caactttatc atcatctttg actttcacaa tatcttcctc aaaataacat ggaataaaga
    35941 tagttttctt ttaaaacggt tgtaggagga aaatctctcc tacactatta ttgattctga
    36001 gagaaaattg caaaatatca tctaccaaac tttcaattat taagattttt tgcagtttat
    36061 ctgaattaga atcgcaaggc ttctcaataa tataattgtc gaattttttc attctaactt
    36121 cctctccatt atttcttcct ttatcaagaa aaagttacct caatattaat aaagtacaat
    36181 tctaaattaa ctccaattct tgattcatga taatatttca gtaactcatc gtgcaattaa
    36241 aaacaaggat ttttatttca gatgatccat cgtgaacact tcatcagcca tctcccaaat
    36301 tgccggatta tgtgttgcga taatgattag cctattatca tctcgaagag atagcaaaat
    36361 ctccataatc aactgagagg ttgctgggtc tattgaagct gttggctcat ctgccagaat
    36421 aaagggtgga ttctttaaga taatttttgc caaggcaacc cgttgcgatt ctccgcccga
    36481 taactcaaag atgcgcttat ctaggtcaag ataagccagg ccgacctgtt ctaaagcctg
    36541 cttctgcctc aaccgctgtt ccgaccgact caacttttga ccaatgagac ctagcttaag
    36601 gttttcttca atactttggt tttcaattaa gccaaagttc tggaagaggt agcccaattc
    36661 gtgacggaaa aaatcactcg atttataatt ggccaagtct ttacctcggt aaaaaatcgt
    36721 cccatcataa ggttctaatt tcccaatcat gttcatcaag gttgtttttc cgctaccact
    36781 tgaaccaatt aaggcataga cttttccagc ctcaaatgtc attgaaagat tcgaaaataa
    36841 ctctcgttct ccaaaagatt tactcacctg tttaagttca atcatattaa cctcccttca
    36901 aaacaagaat ggacatcttg ttttctttct gcatttggac atgtaactgt aaaagagata
    36961 gaccagtaaa gagtaacaag actaagaaag caactcctat ctctacctga agaaatacac
    37021 tcgcaacaaa tcccagtaaa aacacaccca gttgagcaaa gagataagtg cgatggattt
    37081 ctaagaacct gagacctgca atgcgtttga taaaaatggc acgtctaaat tcttcaaagt
    37141 agagcctatt catagtgtta aacaacaaga ttgaagttgc aatcccaagc acagctcctg
    37201 ctagcattac ccaacgttcc ctctggatat tatccaataa tgtgatgtag ttgtggtaac
    37261 ctgtttgcat ttctgagacc caattttcaa tgccttgtct ctggataagc tcctgggcat
    37321 cagacaattg attaaagaga acgtagttct gtactgcgtc tatccaaaac aaaatggact
    37381 gtggaccagt tgattggggt gttataacaa tgatgattgg atctttcaaa aactgctggt
    37441 aagaaatagg ggtcgtatta tacacaaaac gatcctgacc tgattctaaa taacctaccg
    37501 tagcagtcat ttgctgtcgt tcatcttgac tagacatgcg actggttaag tcgtcttcaa
    37561 aaacagattt ataatgttct tcctctgaac ggaggtgttc aggcagcaat aagacaaact
    37621 cccctacatt aaggtgattc atcttttgct caatagttgt atctacagga atgttttgac
    37681 gctccaagta ttgcggtgtg acgataagga cattgccatt cgggttgtaa tcgtgccatt
    37741 ctgtagaggt tatcaagttt ttggaggaag ccatgccatt ttgcaaagta cggtcaatta
    37801 actggtgtct agataagaaa gccttttgtt ctgaaacagc cagatccatc aattgatacc
    37861 aagttctgag tttcctttga gcttgttcat caaaaccagg actcgttcct tcacggctga
    37921 ttgatagggt aatcagttgc ctttcctgac tccaagcctc ttgtccaatc cgatgctgtt
    37981 gccaggcttg ggaatacttt aagctactcc ctattcccag cgttacaata ataatcgcta
    38041 atagttgacc gattaaaatc aaactaatga ttcctctgac aggcacttgc ccttttataa
    38101 tctgcatcag gtgtatcttt tttatcccaa ctgcgaagag ttgagcgaaa aacagggaga
    38161 tcgacaacaa gatgagatta taactgagca agcctgctcc tatggtcatt agggattgcg
    38221 ttggaagcga cagaattttt tgcataagaa tggcgagcac gccagctaaa ctgaaaccaa
    38281 cagctatccc tttcaaatcc tcaccaactg gtctaaggaa aatggaccac cgtttctctc
    38341 ctgaaatgag gcgaatgccc gacgaccgta attggctaat ttgactaatt aaagtcagtg
    38401 ctacaaaggt taagatgaaa atcaatagac tgattaactg aaatccgtta ctaaagatag
    38461 ccattaacgt agatagttta gatggaattg tcaggttcat gttagtcaag ccaagttgac
    38521 ttagctcctc ccttagcaag tgaatatcta ggtgtccatc gaatacaaaa tagtttgttt
    38581 caacactact actttgagca tcttctagtt ttttttcctg aagcccatca ggcaactttc
    38641 cgtccccaaa ggtcgtataa gaaaaaactg tagtaccttc tgagttagaa tcttggtgtt
    38701 gaatcgcaat aaagctatct gtttcttctg ctagtttttc caagcgtgta gcaacatgct
    38761 gaaaagttgt ttcaggggaa gaatcacgta caacaacgtt ggggtaataa tgggagacat
    38821 atgtatcagt ccaaatggta aatacccaaa caaagaacaa actagcaagc aggttggata
    38881 tgagtataaa taattttttc atagtgcatt ccttattcta aaacagaggg cagaaatacc
    38941 cctgccctct ataaactaac aagcttacta aactattgat aaaagaatct tcttcaatta
    39001 ttctttacca tcccgcatca aatgtaacta attttttaat tccacgtttt tttctcatgc
    39061 aatgaaaccc ctttcttttt ccttgt
//
LOCUS       DAVKLX010000015        35687 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKLX010000015 DAVKLX010000000
VERSION     DAVKLX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.015"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            635..856
                     /locus_tag="VW325_000839"
     CDS             635..856
                     /locus_tag="VW325_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9734273.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            1373..1626
                     /locus_tag="VW325_000840"
                     /pseudo
     CDS             1373..1626
                     /locus_tag="VW325_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1754..2983
                     /locus_tag="VW325_000841"
     CDS             1754..2983
                     /locus_tag="VW325_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9734274.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFAGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            3003..5699
                     /gene="ppc"
                     /locus_tag="VW325_000842"
     CDS             3003..5699
                     /gene="ppc"
                     /locus_tag="VW325_000842"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9734275.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5782..5999
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6112..7116
                     /locus_tag="VW325_000843"
     CDS             6112..7116
                     /locus_tag="VW325_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9734276.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7185..8051
                     /locus_tag="VW325_000844"
     CDS             7185..8051
                     /locus_tag="VW325_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734277.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            8048..8806
                     /locus_tag="VW325_000845"
     CDS             8048..8806
                     /locus_tag="VW325_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734278.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8896..10656
                     /gene="dnaG"
                     /locus_tag="VW325_000846"
     CDS             8896..10656
                     /gene="dnaG"
                     /locus_tag="VW325_000846"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9734279.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10659..11768
                     /gene="rpoD"
                     /locus_tag="VW325_000847"
     CDS             10659..11768
                     /gene="rpoD"
                     /locus_tag="VW325_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9734280.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11782..12111
                     /locus_tag="VW325_000848"
     CDS             11782..12111
                     /locus_tag="VW325_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9734281.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12145..13269
                     /gene="cpoA"
                     /locus_tag="VW325_000849"
     CDS             12145..13269
                     /gene="cpoA"
                     /locus_tag="VW325_000849"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9734282.1"
                     /translation="MRKFPLFSSSLSFLLLILFKENDIIVVMEKKKLRINMLSSSEKV
                     AGQGVSGAYRELVRLLHRAAKDQLIVTENLPIEADVTHFHTIDFPYYLSTFQKKRSGR
                     KIGYVHFLPATLEGSLKIPFFLKGIVKRYVFSFYNRMEHLVVVNPMFIEDLVAAGIPR
                     EKVTYIPNFVNKEKWHPLPQEEVVRLRTDLGLSDNQFIVVGAGQVQKRKGIDDFIRLA
                     EELPQITFIWAGGFSFGGMTDGYEHYKKIMENPPKNLIFPGIVSPERMRELYALADLF
                     LLPSYNELFPMTILEAASCEAPIMLRDLDLYKVILEGNYRATAGREEMKEAILEYQAN
                     PAVLKDLKEKAKNISREYSEEHLLQIWLDFYEKQAALGRK"
     gene            13285..14610
                     /locus_tag="VW325_000850"
     CDS             13285..14610
                     /locus_tag="VW325_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9734283.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14641..14817
                     /locus_tag="VW325_000851"
     CDS             14641..14817
                     /locus_tag="VW325_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9734284.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14876..15010
                     /locus_tag="VW325_000852"
     CDS             14876..15010
                     /locus_tag="VW325_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9734285.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            15074..16384
                     /gene="obgE"
                     /locus_tag="VW325_000853"
     CDS             15074..16384
                     /gene="obgE"
                     /locus_tag="VW325_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9734286.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGGDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16394..16552
                     /locus_tag="VW325_000854"
     CDS             16394..16552
                     /locus_tag="VW325_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734287.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            17639..18898
                     /locus_tag="VW325_000855"
     CDS             17639..18898
                     /locus_tag="VW325_000855"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9734288.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            18914..19465
                     /locus_tag="VW325_000856"
     CDS             18914..19465
                     /locus_tag="VW325_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9734289.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            19458..20735
                     /locus_tag="VW325_000857"
     CDS             19458..20735
                     /locus_tag="VW325_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9734290.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20751..21611
                     /locus_tag="VW325_000858"
     CDS             20751..21611
                     /locus_tag="VW325_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9734291.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            21992..22777
                     /locus_tag="VW325_000859"
                     /pseudo
     CDS             21992..22777
                     /locus_tag="VW325_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            22968..25259
                     /gene="pcrA"
                     /locus_tag="VW325_000860"
     CDS             22968..25259
                     /gene="pcrA"
                     /locus_tag="VW325_000860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9734292.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNNLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            25302..25982
                     /gene="radC"
                     /locus_tag="VW325_000861"
     CDS             25302..25982
                     /gene="radC"
                     /locus_tag="VW325_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9734293.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQVSV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDYVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(25979..26668)
                     /locus_tag="VW325_000862"
     CDS             complement(25979..26668)
                     /locus_tag="VW325_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9734294.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYATKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(26686..27327)
                     /locus_tag="VW325_000863"
     CDS             complement(26686..27327)
                     /locus_tag="VW325_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9734295.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(27410..27625)
                     /locus_tag="VW325_000864"
     CDS             complement(27410..27625)
                     /locus_tag="VW325_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9734296.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(27633..27980)
                     /locus_tag="VW325_000865"
     CDS             complement(27633..27980)
                     /locus_tag="VW325_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9734297.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(27985..29100)
                     /locus_tag="VW325_000866"
     CDS             complement(27985..29100)
                     /locus_tag="VW325_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9734298.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPKNQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(29110..30069)
                     /locus_tag="VW325_000867"
     CDS             complement(29110..30069)
                     /locus_tag="VW325_000867"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9734299.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(30182..30751)
                     /locus_tag="VW325_000868"
     CDS             complement(30182..30751)
                     /locus_tag="VW325_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9734300.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            30879..31550
                     /locus_tag="VW325_000869"
     CDS             30879..31550
                     /locus_tag="VW325_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9734301.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            31534..32352
                     /locus_tag="VW325_000870"
     CDS             31534..32352
                     /locus_tag="VW325_000870"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9734302.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            32349..33245
                     /locus_tag="VW325_000871"
     CDS             32349..33245
                     /locus_tag="VW325_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9734303.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            33289..34263
                     /gene="pta"
                     /locus_tag="VW325_000872"
     CDS             33289..34263
                     /gene="pta"
                     /locus_tag="VW325_000872"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9734304.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(34524..35687)
                     /locus_tag="VW325_000873"
                     /pseudo
     CDS             complement(34524..35687)
                     /locus_tag="VW325_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT        10775 a         6352 c         7850 g        10710 t
ORIGIN      
        1 ggtattaaat tattagtttg tatctggatt atgggatttg aatttttcct tcttccaaaa
       61 ctaataaaat tgaaagaaca aaatctagtt taaatgttgc tcgaaaatta aataattgaa
      121 tacgattaac aatgaaacaa tacaacgtga gtcatgactt caaagtaatt gattttatga
      181 ttctggtttc ttacgcttaa ttaagcaagt tgtattcact ttatcaaatt gttattactc
      241 taaaaagcgt ctaacagcta tagagtgtct gtttgcttct ctttactttt attttaaata
      301 attcattaac cttccgtaag tgcccaataa taaggtgtgt tgaagaagtc tgagataaaa
      361 tagttctcag tcacaaaaaa gctatagatt cccattggtg aacagctccc tgtcaagtag
      421 acagtaaaat aatataaaat cttaagcgac ctgattcctg aattccagtg gagtcaggtt
      481 gttttttaac cgtctttctt aatattgtct tacctataat agatatgctt atgagatatg
      541 tttaataaat taattttagt tcaaaatgtc cagttaggac actttgaatg atttcttagt
      601 tttatatatt atacttttct caggagaaag ctagatgtac agacgtttga gagatttgag
      661 ggaagatcac gatttgaccc aaaaacaaat agctaaaata ctttcgttta caaactcagc
      721 ttatgctaaa attgaacggg gtgagcatac gttaacagca gatgtattgg taactctctc
      781 aaacttttac gatgtcagta cagactacct attggaattg acagattttc ctgataaaat
      841 tcgctttaga aaataatctc ctcaatttca tagagtgtga aaatgaggag attttttatt
      901 tgttctttga aaattaaata gtccaaaatg gtactttcct catttgtgga gcaaatttga
      961 atggctcgcc atgataagag cgattttaaa accatcaata aaatagagcg atactttata
     1021 tgccatgata caaatgatat acaatgatac ttctgaccgt tcaggctgcc atcgtaaaag
     1081 agcagcaagt gaaattctta tgatgacttc atcagtcatg ccacgttaaa tgtgtgagtt
     1141 tgttagagaa acgcaattaa tcctcaaaag gttccccgaa ccttttgagt tccacagacg
     1201 catcacgtgg agtgtgtaag cttgttgcta aaagcgtaaa aaccttggaa cgaaaggaat
     1261 aatagacttc ctgcgaaaca aaaatacaat acaataaaac tatggatgat gaagcaagta
     1321 aacaattaag cgatagccgt ttcaagagcc ttgtaggtgt tcagcgcacg agatgttagc
     1381 tgtgttaaaa acagcttatc aacgcaacgt aaacgcgcaa aaggtggacg aaaaaccaaa
     1441 ttaagcctag acgatctcct catggtaact attcaataca tgcgagaata gcgcacttat
     1501 gaacaaattg cggctgattt tggcattcac gaaagcaact taatccgtcg gagccaatgg
     1561 gttgaagtaa ctcttgttca aaatggtttt acgatttcaa attctgccct aattatgaaa
     1621 aacagtaaaa ttcgaaggat tgtaaggtaa gagttttttt ctttctgaaa aaatggtata
     1681 atagcaatca aaactagaaa ataaaacgga atttggaaca gatttgtctg tatcctagta
     1741 gagtggtgat gctatgaaga ttagtaagag gcacttatta aattattcca tcttgattcc
     1801 ctacttgctt ttatctattt tgggcttgat tgtggtctat tcgaccacca gtgctatttt
     1861 aattgaagaa ggcaagagcg ccttgcagtt ggttcgaaac caaggaatct tttggattgt
     1921 tagtttgata ctgattgcct taatttataa attgagacta gattttttga gaaatgagcg
     1981 actaatcatt ttagttatat taatagaaat gcttttattg ttcttggctc gttttattgg
     2041 tatttccgta aacggggcat acggttggat ttcggttgca ggagtaacta ttcagccagc
     2101 tgagtactta aaaatcatta ttatttggta tttagctcac cgattctcca aacagcaaga
     2161 agaaatagct acttatgatt ttcaagtttt gactcaaaat caatggcttc cccgtgcttt
     2221 taatgattgg cgattcgttc tcctagttct gattggaagt ttgggaattt tccctgattt
     2281 aggaaatgcg actattttag tcttggtttc cttgattatg tatacagtta gtggaatcgc
     2341 ttatcgctgg ttttcaacca ttctggcgct cgtatctgcc gcttctgtct ttgtcttgac
     2401 cactatcagc ctaatcggtg ttgagacctt ttcaaaaatt ccagtatttg gctatgtagc
     2461 caagcgcttt agtgcctttt ttaatccttt tgccgatcgt gctgatgcag gtcaccagtt
     2521 agctaattct tattttgcca tggtcaatgg tggttggttt ggtctaggtc ttggaaactc
     2581 gattgaaaaa cgaggttatt tgccagaagc tcatacagac tttgtctttt ctatcgtgat
     2641 tgaagaattt ggctttgctg gtgccagtct tattttagct ctcttgtttt tcatgatttt
     2701 gcggattatc ttggtcggta ttcgagcgga gaatcctttc aatgccatgg ttgcactcgg
     2761 tgtcggaggg atgatgttgg ttcaggtatt tgtcaatatc ggagggattt cgggcttgat
     2821 tccatctaca ggagtaacct tccccttctt atcccagggt ggaaatagtc ttctagtctt
     2881 atcagtggca gtagcctttg tcttaaatat tgatgccagt gaaaaacgcg ctaagttgta
     2941 ccgagaattg gaaaatcaac caatgaacct tctgttgaag taggataaag aaaggatagt
     3001 ttatgtctct tcaaaaatta gaaaattata gtaataaaag tgttgtgcaa gaagaagtct
     3061 tgattctaac agaattactg gaagatatta ctaaaaatat gcttgcccca gagacctttg
     3121 aaaaaataat acagttgaaa gaattatcaa cgcaggaaga ttatcaaggt ctaaaccgtc
     3181 tagtgactag cttatcaaat gatgaaatgg tctatatttc acgctatttc tctatcttac
     3241 ctcttttgat caatatttca gaggatgtgg atttagctta tgaaatcaat catcaaaata
     3301 atattgatca ggactattta ggtaaattat ctacaacgat taaattggta gcagaaaagg
     3361 aaaatgccgt tgagatccta gaacacttga atgttgtccc tgttttgaca gcccatccaa
     3421 cacaagtgca acgcaaaagt atgttggatt taacaaatca tattcatagt cttttgcgta
     3481 aataccgtga tgttaagttg gggttgatca ataaagataa atggtacaat gatttgcgtc
     3541 gttacatcga aattatcatg cagacagaca tgattcgtga gaaaaaatta aaagtgacta
     3601 acgaaatcac gaatgctatg gaatattata acagctcctt tttgaaagct gtacctcatt
     3661 tgacgacgga gtataagcgc ttagcgcaag cgcatggtct gaatttaaaa caggctaaac
     3721 caatcaccat gggtatgtgg ataggtggtg accgtgatgg aaatccattt gttacagcag
     3781 agaccttgaa gcagtctgca ctcactcaat gtgaagtcat catgaactac tatgataaaa
     3841 agatttacca actttatcgt gaattttctc tttcaactag cattgtcaac gtcagcaagc
     3901 aagtcagaga aatggctcgt caatccaagg ataactcgat ttaccgcgaa aaagagcttt
     3961 accgtcgtgc cttgtttgat attcaatcaa aaattcaggc aactaaaacc tatctgattg
     4021 aggatgaaga agttgggact cgttatgaaa ccgccaatga tttctacaag gatttgattg
     4081 ccattcgaga ttctctacta gaaaataagg gtgagtcctt gatttcaggt gattttgtgg
     4141 aattattgca ggcagtagag atatttggtt tttacctagc atcaattgat atgcgacaag
     4201 actctagcgt ctatgaagcc tgtgtggcag aactcttgaa atcagcagga attcattctc
     4261 gttatagcga gttgagcgaa gaagaaaagt gtgaccttct cttgaaagaa ttagaagaag
     4321 atccccgaat tctttctgcg actcacgcag aaaaatcaga attattagca aaagaattag
     4381 ctatttttaa gacggctcgt gttttgaaag ataagttggg agatgatgtc atccgtcaga
     4441 ccatcatttc acatgcaacc agcctttctg atatgctaga attagctatt ctgttaaaag
     4501 aagtaggact ggtggatacg gaaagggcgc gtgttcagat tgttcccctt tttgaaacaa
     4561 ttgaagactt ggatcattca gaagaaacaa tgagaaaata tctttctctt agccttgcca
     4621 aaaaatggat tgactcacga aataactacc aagaaatcat gcttggctac tctgacagta
     4681 ataaagatgg cggttacttg tcatcatgtt ggaccctcta caaggctcaa caacaattga
     4741 ctgctattgg agatgaattt ggcgttaagg ttaccttctt ccatggtcgt ggtggtactg
     4801 tcggtcgtgg tggtgggcca acctatgaag ccattacatc tcaaccgctc aagtctatca
     4861 aggatcgtat ccgcttgacg gagcagggtg aagtaattgg gaataaatac ggtaacaaag
     4921 acgccgctta ctataacctt gaaatgctag tatcggcagc tattaaccgt atgattactc
     4981 agaagaagag cgataccaat accccaaatc gttatgaagc cattatggat caagtagtgg
     5041 accgtagtta cgatatctac cgtgatttgg tctttggtaa tgagcatttc tatgattatt
     5101 tcttcgagtc aagtccaatc aaggctattt caagttttaa tattggttct cgtccagccg
     5161 ctcgtaagac tattactgaa atcggtggtt tgcgtgccat cccttgggta ttctcatggt
     5221 cacagagtcg tgttatgttc cctggatggt acggggttgg ttcaagcttc aaggaattta
     5281 tcaataaaaa tccagagaat attgctatct tacgagatat gtaccaaaat tggcctttct
     5341 tccaatcgct tctttcaaat gttgatatgg ttttgtcaaa atcaaatatg aatattgctt
     5401 ttgaatatgc taaactttgt gaagacgagc aagttaaggc catctatgag actattttaa
     5461 atgaatggca agttactaag aacgttatct tggctattga aggacatgac gaactcttag
     5521 ctgacaatcc atatctaaaa gctagtctgg attaccgtat gccttacttt aatattctca
     5581 actatattca gttagagttg attaaacgcc aacgtcgtgg agaattgtcc agtgatcaag
     5641 aacgattgat tcatatcacc atcaacggaa ttgcgacagg attgcgtaat tcaggttgat
     5701 aattttcaag agtgaatgct aaaagtgaat atcaaaaaaa ttctaataga ctattgacaa
     5761 gtagtttaaa aatgatataa tttaaccatt cagaaaagta atcatacaaa ctttttagag
     5821 agtctgtggt agctgaaaac agataagtgg cgatgatgaa aattgggctg aatgctattt
     5881 agaatttgaa attataaaaa ttcggtaagc acaccttaca gtgcatctcg ttattgcgag
     5941 actgagcgat agggaaattc cctataattg aggtggtacc gcgcatcgac gtcctcacac
     6001 aagttttttg tgtgaggatt tttttgatgg aggttagtat ggaaagaaaa cgatggcgtc
     6061 gcttgtttag ataagtgaaa tatgttaaag gaaataaaaa ggagaaacag aatgaaaaat
     6121 aaacgtttaa ttggaattat tgctgcatta gcagtcttag tagcaggaag cttgatttat
     6181 tcttcaatga ataaatcaga agctcagaat aataaggatg agaagaaaat aactaagatt
     6241 ggtgtgcttc aatttgtgag ccatccatcc cttgatttga tttataaagg gatccaagat
     6301 ggacttgcag aagaaggata taaagatgat caagttaaaa ttgattttat gaactcagaa
     6361 ggtgaccaaa gtaaggttgc gacaatgagt aaacaattgg ttgcaaatgg gaatgacctt
     6421 gtggttggta tcgcaacacc agcagcccaa gggttggcta gtgcaacaaa agacctaccg
     6481 gttatcatgg ccgctattac agacccaatt ggtgctaact tggttaaaga tttgaaaaaa
     6541 ccaggtggca acattacagg ggtatctgac cacaatccag ctcaacaaca agttgaactc
     6601 atcaaggctc tgacaccgaa tgtgaaaaca atcggagctc tttactcaag tagcgaagac
     6661 aattcaaaaa cacaggtcga agaatttaag gcttatgctg aaaaagcagg tctgacagtg
     6721 gaaacatttg cagttccttc aacaaatgaa attgcctcaa ctgtcactgt tatgactagc
     6781 aaggtagatg ctatttgggt tccaattgat aacaccattg catcaggatt tccaacggtt
     6841 gtctctagca atcaaagttc taagaaacca atttatccca gtgcgacagc tatggtagaa
     6901 gtaggtggtt tggcatcagt tgtaattgac caacatgacc ttggtgtggc aacaggtaaa
     6961 atgattgtgc aagtcttgaa aggtgcaaaa ccagccgata ccccagtcaa tgtcttttca
     7021 actggtaagt cagtcatcaa taaaaaaata gcacaagaac taggtattac tattcctgag
     7081 tctgttctca aagaagcagg acaagtcatc gaataaataa ggtagggagg aggagactct
     7141 tcccattttt taaaaagatg aatatagaaa ggattaaata aaatatgatt gtttccatta
     7201 tttctcaagg atttgtctgg gctattctag gtctgggaat ctttatgaca tttaggattt
     7261 taaactttcc agatatgacg acagaaggtt ccttccctct tgggggagct gttgctgtca
     7321 ctttgataac caaaggcgtg aacccatttt tagcgacact tgttgctgta ggagcaggtt
     7381 gtttggctgg aatggcagca ggccttcttt atacaaaagg gaagatccca accttgctct
     7441 cagggatttt ggtgatgact tcttgtcact caatcatgct cttgattatg ggacgtgcga
     7501 atttaggcct gcttggaacc aagcaaattc aggatgtttt gccttttgat tcggatttga
     7561 atcaactctt gacaggtctc atctttgtga gtattgttat tgctctcatg ctctttttct
     7621 tggacactaa actcggacaa gcctatattg ctacagggga taatcctgat atggctagaa
     7681 gtttcgggat tcatactgga cgcatggagc tcatgggctt ggtcttatca aatggtgtga
     7741 ttgcccttgc aggtgccctc attgctcagc aagaaggtta tgccgatgtg tctcgaggga
     7801 tcggggttat cgttgtgggg cttgcaagtt tgattattgg agaagttatt ttcaagagtt
     7861 tgagcttggc agagcgtttg gttactatcg ttgtaggttc tatcgcttat caatttttag
     7921 tgtgggcagt tatcgcactt ggctttaata caagttacct tcgtttatac agtgccttga
     7981 ttttagcagt ctgcctcatg attccaacat ttaagcaaac aatcttgaaa ggagccaagt
     8041 taagcaaatg acagcaattg tagaattaaa aaatgcaacc aaaatcgtta aaaatggctt
     8101 tgatgaagaa aagattattt taaatgatgt ttccttagaa atttttgaac gggactttat
     8161 cacgattttg ggcggaaatg gtgctggaaa atcaaccctc tttaacacta tagcagggac
     8221 cttatcacta actagtggaa ctatccgtat tttaggtgaa gaactcacta agttttcacc
     8281 cgagaagcgt gccaagtacc tgtctcgtgt cttccaagat ccaaagatgg ggacagctcc
     8341 ccgtatgacg gtcgctgaaa atcttttaat cgccaagttt cgtggtgaaa agcgtggatt
     8401 gttaccacga cgcttgacta gctataagga tgaatttcag gcaaccattg aaaaagtagg
     8461 aaatggtctt gagaaacact tgaatacacc gattgagttc ttatcaggtg gacaaagaca
     8521 ggctttgagt ctcttgatgg caaccttgaa gcgacctgaa ttactcctgt tagatgagca
     8581 tactgctgcc ctggatccaa agactagtgt tgctttgatg gaattgacag atgaatttgt
     8641 taagaaagat cagctaacag cccttatgat tactcatcat atggaagatg ctctcaaata
     8701 cggcaatcgc ttgattgtca tgaaagaagg acggattatc caagatttaa accaagaaga
     8761 aaaagcaaaa atgaaaatct ctgattatta tcaactcttt gaataaagta gggaaaatga
     8821 gtgaaatggt ttactttttt tctgaaataa agtatactat ataaagtaaa ctatgataac
     8881 atggaggtat tgtgtatggt tgacaaacaa gtcattgaag aaatcaaaaa caatgccaac
     8941 attgtggaag tcataggaga tgtgatttct ttacaaaagg caggacggaa ctatctaggg
     9001 ctctgtcctt ttcatggtga aaaaacacct tctttcaacg ttgtagagga taagcagttt
     9061 tatcactgtt tcggttgtgg tcgctcaggt gatgtcttta agttcatcga ggagtaccaa
     9121 ggggttccct ttatagaggc agtccaaatc ttaggtcagc gtgttggaat tgaggttgaa
     9181 aaaccgcttt atagtgagca gaagccagcc tcacctcacc aagctcttta tgatatgcac
     9241 gaagatgcag ccaaatttta ccatgcgatt ctcatgacaa cgactatggg cgaagaggcc
     9301 agaaattacc tttatcagcg ggggttgaca gatgaagtgc ttaaacattt ttggattggt
     9361 ttagcacctc cagaacgaaa ctatctctat caacgtttgt ctgatcagta tcgtgaagag
     9421 gatttactgg attcaggcct gttttatctc tcggatgcca atcaatttgt agacaccttt
     9481 cacaatcgca ttatgtttcc cctgacaaat gaccaaggaa aggtcattgc cttctcaggt
     9541 cgtatctggc aaaaaacgga ttcacaaact tctaagtata aaaacagccg atcgactgca
     9601 atttttaaca aaagttacga attatatcat atggataggg caaaaaaatc ttctggaaaa
     9661 gctagtgaga tttacttgat ggaaggattc atggatgtta ttgcagccta tcgggctgga
     9721 atcgaaagtg ctgtggcgtc gatgggaacg gccttgagtc gagagcatgt tgagcatctg
     9781 aaaaggttaa ccaagaaatt ggttcttgtt tacgatggag ataaggctgg gcaagccgcg
     9841 acattgaaag cattggatga aattggtgat atgcctgtgc aaatcgtcag catgcctgat
     9901 aacttggatc ctgatgagta tctacaaaaa aatggtccag aagacttggc ctatctatta
     9961 acgaaaactc gtattagtcc gattgagttc tacattcatc agtacaaacc tgaaaacagt
    10021 gaaaatctgc aggctcagat tgagtttctt gaaaaaatag ctcccttgat tgttcaagaa
    10081 aagtccatcg ctgctcaaaa cagctatatt catattttag ctgacagtct ggcgtccttt
    10141 gattataccc agattgagca gattgttaat gagagtcgtc aggtgcaaag gcagaatcgc
    10201 atggaaggaa tttccagacc gacgccaatc accatgccaa tcaccaagca gttatcggct
    10261 attatgaggg cagaagccca tctactctat cggatgatgg aatcccctct tgttttgaac
    10321 gattaccgtt tgcgagaaga ctttgcattt gctacacctg aatttcaggt cttatatgac
    10381 ttgcttggcc agtatggaaa tcttcctcca gaagttttag cagagcagac agaggaagtt
    10441 gaaagagctt ggtaccaagt tttagctcag gatttgcctg ctgaaatatc gccgcaggaa
    10501 cttagtgaag tagagatgac tcgaaacaag gctctcttga atcaggacaa tatgagaatc
    10561 aaaaagaagg tgcaggaagc tagccatgta ggagatacag atacagccct agaagaattg
    10621 gaacgtttaa tttcccaaaa gagaagaatg gagtaataat ggcaacaaaa caaaaagaag
    10681 taacaacatt tgacgtacag gtagcagaat ttatccgtaa tcataagcaa aaagggacag
    10741 caacagatga tgaaatcaat gcaagtctgg ttattccttt taccttggac gctgatggga
    10801 ttgaagatct cttgcaacgg attcaggatg caggaatttc tatcacagat aacgaaggaa
    10861 atccaagtgc gcgtgttctt agcaatgaag aagaaccaga actcagcgat gaggacttga
    10921 ttgggtcaac ttctgctaag gtcaatgacc ctgtccgtat gtacttgaaa gaaatagggg
    10981 tcgttcctct cttgaccaat gaagaggaga aagagttggc actggctgtt gaagctggtg
    11041 atatcgaagc caaacaacgt cttgcggaag ccaatcttcg tttggttgtt tccattgcca
    11101 aacgctatgt cggtcgtggt atgcagttcc ttgacttgat tcaagaagga aatatgggct
    11161 tgatgaaggc ggttgacaag tttgactatt ctaaagggtt caagttttca acttatgcaa
    11221 cttggtggat tcgtcaggct atcactcgtg ctattgcgga ccaagctcgt accatccgta
    11281 tcccagttca catggttgaa actatcaata aattggttcg tgaacagcgg aatctccttc
    11341 aagaattggg gcaagatccg acaccagaac agattgctga acgaatggat atgacacctg
    11401 ataaggttcg tgaaatcttg aagattgccc aagaaccagt atctcttgaa actcctatcg
    11461 gtgaagagga cgatagccac cttggagact ttatcgaaga tgaagtgatt gaaaatccag
    11521 tggattatac gactcgtatc gtcttgcgtg agcaattgga tgaaatctta gatactctta
    11581 cagaccgtga agaaaatgtt ctgcgtctac gttttggact agatgatgga aaaatgcgca
    11641 cacttgaaga tgtggggaaa gtctttaacg taactcgtga gcgtatccgt cagattgaag
    11701 caaaggcttt gagaaaacta cgccaaccaa gtcgtagcaa accgcttcgt gattttattg
    11761 aagactaaga gtgaggaaaa tatggcttat acagaagagc aaattgaaaa catcaaaaca
    11821 cggattttaa cagccttgga agaagtcatc gaccctgagt tgggaatcga tattgtcaat
    11881 cttggtttga tctatgagat tcgttttgac ggtgacacag ggcaaacaga gattgacatg
    11941 actttgacaa ctatgggttg tcccttagca gaccttttga cagaccagat ttatgatgct
    12001 atgatagagg ttccagaagt aacggatact gaggttaaat tagtctggta tccagcctgg
    12061 acagttgaaa aaatgagtcg ctatgcccgc attgccctag gcattaagta aacatataaa
    12121 agcatgtgag agactgttgg aaaagtgagg aaatttcccc tcttttcctc tagtctctcc
    12181 tttcttttgc tgattttatt caaagaaaat gatataatag tagttatgga gaaaaagaaa
    12241 ttacgcatca atatgttgag ttcaagtgag aaagtagcag gacagggagt ttcaggtgct
    12301 taccgtgaat tagttcgtct tcttcaccgt gctgccaagg accaattgat tgttacagaa
    12361 aatcttccaa tcgaggcaga tgtgactcac tttcatacga ttgattttcc ctattattta
    12421 tcaaccttcc aaaagaaacg ctcagggaga aagattggct atgtgcattt cttgccagct
    12481 acacttgagg gaagtttgaa aattccattt ttcttaaagg gaattgtgaa acgctatgta
    12541 ttttcttttt acaaccggat ggagcacttg gttgtggtca atcctatgtt tattgaggat
    12601 ttggtagcag ctggtattcc acgtgaaaaa gtgacctata ttcctaactt tgtcaacaag
    12661 gaaaaatggc atcctctacc acaagaagag gtagttagac tgcgcacaga tcttggtctt
    12721 agtgacaatc agtttatcgt agtaggtgct gggcaagttc agaaacgtaa agggattgat
    12781 gactttatcc gtctggctga ggaattgcct cagattacct ttatctgggc tggtggcttc
    12841 tcttttggtg gtatgacaga tggttatgaa cactataaga aaattatgga aaatccccct
    12901 aaaaatttga tttttccagg cattgtatcg ccagagcgga tgcgcgaatt gtatgctcta
    12961 gcggatcttt tcttgttgcc tagttacaat gagctctttc ctatgactat tttagaagct
    13021 gcgagttgtg aggctcctat tatgttgcgt gatttagatc tctataaggt gattttggag
    13081 ggaaattatc gggcgacagc gggtagagaa gagatgaaag aggctatttt ggaatatcaa
    13141 gcaaatcctg ctgtcttaaa agatctcaaa gaaaaggcta agaatatttc cagagagtat
    13201 tctgaagagc atctgttaca aatctggttg gacttttatg agaaacaagc cgctttaggg
    13261 agaaagtaaa aagtgaggta atctatgcga attggtttat ttacagatac ctattttcct
    13321 caggtttctg gtgttgcgac cagtattcga accttgaaaa cagaacttga aaagcaggga
    13381 catgctgttt ttatctttac gacgacagat aaggatgtca atcgctacga agattggcaa
    13441 attatccgca ttccaagtgt tcctttcttt gcttttaagg atcgtcgctt tgcctaccga
    13501 ggttttagca aggcacttga aattgctaaa cagtatcagc tagatattat ccatactcag
    13561 acagaatttt ctcttggcct gttggggatt tggattgcgc gtgaattgaa aattccagtc
    13621 atccatacct atcacaccca gtatgaagac tatgtccatt atattgctaa ggggatgttg
    13681 atccggccga gtatggtcaa gtatctggtt agaggtttcc tgcatgatgt ggatggggtt
    13741 atttgcccta gtgagattgt ccgtgacttg ctatctgatt ataaggtcaa ggttgaaaaa
    13801 cgggtcattc ctactgggat tgaattagcc aagtttgagc gtccggaaat caagcaggaa
    13861 aatttgaaag aactgcgtag taaactaggg attcaagatg gtgaaaagac gttgcttagt
    13921 ctttcgagaa tctcctatga aaaaaatatt caagcagttt tagcagcctt tgctgatgtt
    13981 ctgaaagagg aagacaaggt taaactggta gtagctgggg atggccctta tctgaatgac
    14041 ctcaaagagc aagctcagaa cctagagatt caagactcag tcatctttac agggatgatt
    14101 gctcctagtg agacggctct ttactataaa gcggcggatt tcttcatttc ggcatcgaca
    14161 agcgaaacgc aaggtttgac ctacttggaa agcttagcca gtggaacacc tgtcattgct
    14221 cacggaaatc cttatttgaa caacctcatc agtgataaaa tgtttggaac cttgtactat
    14281 ggagaacatg atttggctgg tgctattttg gaagccctga ttgcaacacc agacatgaac
    14341 gagcatacct tatcagagaa attgtatgag atttcagctg agaactttgg gaaacgagtg
    14401 catgagtttt atctggatgc cattatttca aataacttcc agaaagattt ggctaaagat
    14461 gatacggtca gtcagcgtat ctttaagaca gttttgtatc ttcagcaaca ggtggttgct
    14521 gtacctgtaa aaggatctag acgcatgttg aaggcttcaa aaacacagtt gatcagtatg
    14581 agagactatt ggaaagacca tgaagaatag aaagaggaac agctatgaaa aaaacaatta
    14641 atgagaagcg gtcgtgataa aaagattgcg ggtgtttgtg ctggggtggc ccattatctg
    14701 gatatggatc cgactatcgt tcaagtcatt tggggtgttc ttacttgctg ttacggagct
    14761 ggaattgtag cttacattat tttatggatt atcgcaccag tagcaacaga ctattgaaaa
    14821 ctagctcaat tcatgctaag ataatagaaa atagaatgta acgaaggaga gaaaaatggc
    14881 atttggagat aatggaaatc gtaaaaaaac tatgtttgag aaaataacct tgtttatcgt
    14941 gattatcatg ctagtagcaa gtttattggg aatttttgca actgcaattg gtgccctcag
    15001 taatctataa aatagattca agaaaattta gtgactggga tttcccagcc cttttttaaa
    15061 gtgagaagaa ataatgagta tgtttttaga tacagctaag attaaggtca aggctggtaa
    15121 tggtggcgat ggtatggttg cctttcgtcg tgaaaaatat gtccctaatg gaggcccttg
    15181 gggtggtgat ggtggtcgtg gaggcaatgt ggtcttcgtt gtagacgaag gactacgtac
    15241 cttgatggat ttccgctaca atcgtcattt caaggctgat tctggtgaaa aagggatgac
    15301 caaagggatg catggtcgtg gtgctgagga ccttagagtt cgagtaccac aaggtacgac
    15361 tgttcgtgat gcggagactg gcaaggtttt aacagatttg attgaacatg ggcaagaatt
    15421 tatcgttgcc cacggtggtc gtggtggacg tggaaatatt cgtttcgcga caccaaaaaa
    15481 tcctgcaccg gaaatctctg aaaatggaga accaggtcag gaacgtgagt tacaattgga
    15541 actaaaaatc ttggcagatg tcggtttagt aggattccca tctgtaggga agtcaacact
    15601 tttaagtgtt attacctcag ctaagcctaa aattggtgcc taccacttta ccactattgt
    15661 accaaattta ggtatggttc gcacccaatc aggtgaatcc tttgcagtag ccgacttgcc
    15721 aggtttgatt gaaggggcta gtcaaggtgt tggtttggga actcagttcc tccgtcacat
    15781 cgagcgtaca cgtgttatcc ttcacatcat tgatatgtca gctagcgaag gccgtgatcc
    15841 atatgaggac tacctagcta tcaataaaga gctggagtct tacaatcttc gcctcatgga
    15901 gcgtccacag attattgtag ctaataagat ggacatgcct gagagtcagg aaaatcttga
    15961 agaatttaag aaaaaattgt ctgaaaatta tgatgaattt gaagagttac cagctatctt
    16021 cccaatttct ggattgacca agcaaggtct ggcaacactt ttagatgcta cagctgaatt
    16081 gttagacaag acaccagaat ttttgctcta cgacgagtcc gatatggaag aagaagctta
    16141 ctatggattt gacgaagaag aaaaagcctt tgaaattagt cgtgatgacg atgcgacatg
    16201 ggtactttct ggtgaaaaac tcatgaaact ctttaatatg accaactttg atcgtgatga
    16261 atctgtcatg aaatttgccc gtcagcttcg tggtatgggg ggtgatgaag cccttcgtgc
    16321 gcgtggagct aaagatgggg atttggtccg cattggtaaa tttgagtttg aatttgtaga
    16381 ctaggagact ggtatgggag ataaaccgat atctttccga gatgcggatg gtaattttgt
    16441 ttccgccgca gacgtttgga atgaaaagaa attggaagaa ctatttaatc gtctcaatcc
    16501 aaatcgtgcc ttgagattgg cacgaactaa aaaggaaaat ccatctcagt aaagaagcta
    16561 aaaaatcccg tgcctcatca gacacgggat tttgtggtac gacaggcatg tatagcaaac
    16621 tgaatctgga atagcacagc atatcttcta aaatattgct ataaattgat ttgaatttca
    16681 aaattaaatt gttttattct tatttcaatt tgttatagta tatctgatgt caaagttctc
    16741 ggcgagtcaa atagtgattc ccaagcctga ctatcgtgag gtagcggatt aaaatggtct
    16801 ggggatagac cgttttaagt ctgatgctgg aaataagaat tgtcagaaga agggatagcg
    16861 aaatcgtggc tctacgaaca ggaacgtgat aataaggcgt atatagcgaa taagagggcc
    16921 tcaaactcta aagtccaaaa aggtagtcgt aacctatatg cgtaaatcat gagagtaatt
    16981 gaattcgtac taagattttc tatttttact gtaacctttt aacgtcctca tatcttgtat
    17041 acacgaggaa agatgtacga cttatcccgt gaggtctatc actataaaga gaaaacgaca
    17101 gatagaagtg atcctgagtc acggttatct ttaaaataga ttgtctgata ggatgctagc
    17161 ctgaaaacgt cttgttcatg aaatatagga cggtatgtat aaaacgcttc tgtgaactga
    17221 gagaaggggg agaagttctt gctaaaattt agttgaacag ccgtattccg atacttagat
    17281 aagagatcta gtcttagctc ctactcagtt tcaggggata aaaaaggggc aatagcgatt
    17341 cgagaaagat tatactcttc gaaaatctct tcaaatcacg tcaatatcgc cttgccgtat
    17401 gtgtaggata ctaactacgt cagttccatc tacaatctca aaacagtgtt ttgagcaacc
    17461 tgcggctagt ttcctagttt gatctttgat ttttattgag tattagtaat tcagttacta
    17521 actcgtcaac tctgatttat ccaataaaat tgaaaaggat ggaaaaaagg ataaatttat
    17581 gatatacttt attttgaaga ccttattaga aatcttgaaa gagtattgaa aacttagaat
    17641 gagaaaaatt gttatcaatg gtggattacc actgcaaggt gaaattacta ttagtggtgc
    17701 taaaaatagt gttgtggcct taattccagc tattatattg gctgatgatg tggtgacttt
    17761 ggattgtgtt ccagatattt cggatgtagc cagtcttgtc gaaatcatgg aattaatggg
    17821 agctactgtt aagcgttatg acgatgtctt ggagattgat ccaagaggtg ttcaaaatat
    17881 tccaatgcct tatggtaaaa ttaacagtct tcgtgcatct tactattttt atgggagcct
    17941 cttaggccgt tttggtgaag cgacagttgg tctaccggga ggatgtgatc ttggtcctcg
    18001 tccgattgac ttacacctta aggcgtttga agctatgggt gccactgcta gctacgaggg
    18061 agataacatg aagttatctg ctaaagatac aggacttcat ggtgcaagta tttacatgga
    18121 tacggttagt gtgggagcaa cgattaatac gatgattgct gcggttaaag caaatggtcg
    18181 tactattatt gaaaatgcag cccgtgaacc tgagattatt gatgtagcta ctctcttgaa
    18241 taatatgggt gcccatatcc gtggggcagg aactaatatc atcattattg atggtgttga
    18301 aagattacat gggacacgtc atcaggtgat tccagaccgc attgaagctg gaacatatat
    18361 atctttagct gctgcagttg gtaaaggaat tcgtataaat aatgttcttt acgaacacct
    18421 ggaagggttt attgctaagt tggaagaaat gggagtgaga atgactgtat ctgaagacag
    18481 catttttgtc gaggaacagt ctaatttgaa agcaatcaat attaagacag ctccttaccc
    18541 aggctttgca actgatttgc aacaaccgct tacccctctt ttactaagag cgaatggtcg
    18601 tggtacaatt gtcgatacga tttacgaaaa acgtgtaaat catgtttttg aactagcaaa
    18661 gatggatgcg gatatttcga caacaaatgg tcatattttg tacacgggtg gacgtgattt
    18721 acgtggggcc agtgttaaag cgaccgactt aagagctggg gctgcactag tcattgctgg
    18781 gcttatggct gaaggtaaaa ctgaaattac caatatcgag tttatcttac gtggttattc
    18841 tgatattatc gaaaaattac gtaatttagg agcggatatt agacttgttg aggattaaac
    18901 cgtagaggtg tttatgaata tttggaccaa attagcaatg ttttcttttt ttgaaacgga
    18961 tcgcttgtat ttgcgtcctt tcttttttag tgatagtcag gacttccgcg agatagcttc
    19021 aaatccagaa aatcttcaat ttattttccc aacgcaggca agtctggaag aaagtcaata
    19081 tgcactggcc aattacttta tgaagtcccc tttgggagtg tgggcaattt gtgaccagaa
    19141 aaatcaacaa atgattggtt ctattaaatt tgagaagtta gatgaaatca aaaaagaagc
    19201 tgagcttggc tattttttga gaaaagatgc ttggtcgcaa ggatttatga cagaggttgt
    19261 tagaaaaatt tgtcagcttt cttttgagga atttggctta aaacaattat ttatcattac
    19321 ccaccttgaa aatgaagcta gccaaagagt tgctcttaag tctggattta gtttgttccg
    19381 tcagtttaag ggaagtgatc gttacacaag aaaaatgcgg gattatcttg aatttcggta
    19441 tgtaaaagga gagttcaatg agtaagcatc aggaaattct aagctatttg gaggaattac
    19501 cagtaggtaa aagggtcagt gttcgtagca tttcgaatca tctaggagtt agtgatggaa
    19561 cagcctatcg agctattaaa gaagctgaaa accgtggaat tgtggagacc cgtcctagaa
    19621 gtggaacaat tcgtgttaaa tcccagaaag ttgctataga gagattaacg tttgctgaaa
    19681 ttgcagaagt gacttcttct gaggttctgg ctgggcaaga aggtttagag agagagttta
    19741 gtaagttttc aattggtgcc atgactgaac aaaatatctt gtcttacctt catgatgggg
    19801 ggctcttgat tgtcggagac cgaacccgta ttcagttgct agccttggaa aatgaaaatg
    19861 cagttctggt tacaggggga tttcaggttc atgatgatgt gcttaaactg gccaatcaaa
    19921 aagggattcc tgttctaaga agtaagcatg atacctttac cgtcgcgacc atgatcaata
    19981 aagccttgtc aaatgtccaa atcaagactg atattctgac agttgagaaa ctttatcgcc
    20041 ctagtcatga gtatggtttt ctgagagaga cagatacagt taaagattat ttggacttgg
    20101 ttcgtaagaa tcgtagcagc cgtttccctg ttatcaatca acatcaggtc gttgttggcg
    20161 ttgtaaccat gagagacgct ggtgataaat caccaagcac gacaattgat aaggttatgt
    20221 ctcgtagtct atttttggtt gggttatcga caaatattgc caatgtgagt caacggatga
    20281 tcgcagaaga ctttgaaatg gtaccagttg ttcgaagcaa tcaaactttg cttggcgttg
    20341 tgacgcgacg agatgtcatg gagaagatga gccgttccca agtttcggct ctaccaactt
    20401 tttctgagca gattggacaa aagctctctt atcaccatga tgaagtagtc attacagtgg
    20461 aaccctttat gctagaaaaa aatggagttt tggctaatgg tgtattggca gaaattctga
    20521 cccacatgac ccaagattta gttgttaata gtggtcgcaa tctcattatc gagcagatgc
    20581 tgatctactt tttgcaggct gttcagatag atgatatatt gcgcattcag gcacggatta
    20641 ttcatcatac gagacggtca gctataattg attacgatat ttatcatggt caccagattg
    20701 tttcaaaagc aaatgtgact gttaaaatta attagaaact aggagaaaag atgataacat
    20761 taaaatcagc tcgtgaaatc gaagctatgg acaaggctgg tgattttcta gcaagtattc
    20821 atataggctt acgtgatttg attaagccag gcgtagatat gtgggaagtt gaagaatatg
    20881 tccgccgtcg ttgtaaagaa gaaaatttcc ttccacttca gattggggtt gacggtgcca
    20941 tgatggacta tccttatgct acctgttgct ctcttaacga tgaagtggct cacgctttcc
    21001 ctcgtcatta tatcttgaaa gatggtgatt tgctcaaagt tgatatggtt ttgggaggtc
    21061 ccattgctaa atctgacctg aatgtctcaa aattaaactt caacaatgtt gaacaaatga
    21121 aaaaatacac tcagagctat tctggtggtt tagcagactc atgttgggct tatgctgttg
    21181 gtacaccgtc cgaagaagtc aaaaacttga tggatgtaac caaagaagct atgtacaagg
    21241 gtattgagca agctgttgtt ggaaatcgta tcggtgatat cggtgcggct attcaagaat
    21301 acgctgaaag tcgtggttac ggtgtagtgc gtgatttggt tggtcatggt gttggcccaa
    21361 ctatgcacga agaaccaatg gttcctaact atggtattgc aggtcgtgga ctccgtcttc
    21421 gtgaaggaat ggtcttaacc attgaaccaa tgatcaatac aggcgattgg gaaattgata
    21481 cagatatgaa aactggttgg gcgcataaga ccattgacgg tggattgtca tgtcagtatg
    21541 aacaccaatt tgtcattacg aaagatggac ctgttatctt gactagccaa ggtgaagaag
    21601 gaacttatta ataaaaagtg aaaagactac tggaagttta ttttgataaa aaatccagta
    21661 gatcttttca taataaaacg cattgtatca agtgttagga gctgatatca tgcgtttttc
    21721 tgcttttaag attttttcca actctgtttg taagcgcatc ataacaaagg gtctaggatt
    21781 cagggctctc ctcctatata ctattagtaa agtaaaacta agggaggata ttttagtgtc
    21841 gcagtctatt gttcctgtag agattccaca atattgtcgt tttgattcta aaaagagaaa
    21901 tggaattctg tttaacgttc gtattgccaa tcttaaattt acctttttta gattagactc
    21961 cctgcgaaac aaaatatggt atagtagttc tatgaatgat gaagcaagta aacaactaac
    22021 tgatgcacga tttaagcgtc ttgttggtgt tcagcgcacg acttttgaag agatattagc
    22081 tgtattaaaa acagcttatc aacttaaaca cgcaaaaggt ggacgaaaac ctaaattaag
    22141 cctagaagac cttcttatgg ccactcttca ctatgtgcga gaatatcgaa cttatgaaga
    22201 aattgcggct gattttggta tccacgaaag caacttactc cgtcggagcc aatgggttga
    22261 agtaactctt gttcaaaggt ggtgttacga tttcaagaac tcctctcagc tctgaggaca
    22321 cggtaatgat tgatgcgacg gaagtaaaaa tcaatcgccc taaaaaagaa ttagcgaatc
    22381 attctggtaa aaagaaattt cacgctatga aggctcaagc gattgtcaca agtcaaggga
    22441 gaattgtttc tattgtcatg atatgaagtt gttcaaaatg agtcgcagaa atattggaca
    22501 agctggtaaa atcttggctg acagtggtta tcaagggctc atgaagatat atcctcaagc
    22561 acaaactcca cgtaaatcca gcaaactcaa gccactaata gctgaagata aagcttataa
    22621 ccatgcgcta tctaaggaga gaagcaaggt tgagaacatc tttgccaaag taaaaacgtt
    22681 taaaatgttt tcaacaacct atcgaagtca tcgtaaacgc ttcggattac gaatgaattt
    22741 gagtgctggt attatcaatc atgaactagg attctagttt tgcaggaagt ctattgaggt
    22801 attgagctag tttatgaaaa aattgggtga aaagtcgagt gttttagaaa cccacagtgt
    22861 agtattctag tttcaatcca ctatattttg ctactccccg taaagtttct attttccctg
    22921 atttctgata taatagaaat attgacttca agagtaagga agagaagatg aacgcattat
    22981 taaatggaat gaatgaccgt caggctgagg cggtgcaaac gacagaaggt cccttgctaa
    23041 tcatggcagg ggctggttct ggaaagactc gtgttttaac ccaccgtatc gcttatttga
    23101 ttgatgaaaa gctggtcaat ccttggaata tcttggccat tacctttacc aacaaggctg
    23161 cgcgtgagat gaaagagcgt gcttatagcc tcaatccagc tactcaggac tgtctgattg
    23221 cgaccttcca ctccatgtgt gtgcgtattt tgcgtcgcga tgcggaccat attggctaca
    23281 atcgtaattt tacaattgtg gatcctggtg aacagcgaac gctcatgaaa cgtattctca
    23341 aacagttgaa cttggatcct aaaaaatgga atgaacgaac tattttgggg accatttcca
    23401 atgctaagaa taatttgatt gatgatgttg cttatgctgc ccaagctggc gatatgtata
    23461 cgcaaattgt ggcccagtgt tatacagcct atcaaaaaga acttcgtcag tccgaatccg
    23521 ttgactttga tgatttgatt atgctgacct tgcgtctctt tgatcaaaat cctgatgttt
    23581 tgacctacta ccagcaaaaa ttccaataca tccacgttga tgagtaccaa gataccaacc
    23641 acgctcagta ccaattggtc aaactcttgg cttcccgttt taaaaatatc tgtgtggttg
    23701 gggatgcgga ccagtctatc tacggttggc gtggtgctga tatgcagaat atcttggact
    23761 ttgaaaagga ttaccccaaa gccaaggttg ttttgttgga ggaaaattac cgctcaacca
    23821 aaaccattct ccaagcggcc aacgaggtta ttaaaaataa taaaaatcgc cgtcctaaaa
    23881 atctctggac tcaaaacgct gatggggagc aaatcgttta ctatcgtgct gatgatgagc
    23941 tggatgaggc tgtatttgta gccagaacca tcgatgaact tagtcgcagt caaaacttcc
    24001 ttcataagga ttttgcagtt ctctatcgga ctaatgccca gtcccgtaca attgaggaag
    24061 ccctgctcaa gtctaacatt ccttatacca tggttggcgg aaccaaattc tacagccgta
    24121 aggaaattcg cgatattatt gcttatctca accttattgc taatttgagt gacaatatta
    24181 gttttgagcg tattatcaac gagcctaaac gtggaattgg tctaggtaca gttgagaaaa
    24241 tccgtgattt tgcaaatttg caaaatatgt ctatgctgga tgcttctgct aatattatgt
    24301 tgtctggtat caagggtaag gcagcccaat ctatctggga ttttgccaat atgatgcttg
    24361 atttgcggga gcagctagac cacttaagca ttacagagtt ggttgagtcc gtcctagaaa
    24421 aaacaggtta tgtcgatatt cttaacgccc aagcgactct agaaagcaag gcacgggttg
    24481 aaaatatcga agagtttctt tctgttacga agaactttga tgacaccacg gatgtgacag
    24541 aagaggaaac tggtctggac aaactgagtc gtttcttaaa tgacttggct ttgattgccg
    24601 acacagattc aggtagtcag gagacatcag aagtgacctt gatgaccctg catgctgcca
    24661 aaggtctcga atttccagtt gtctttttga ttgggatgga agaaaatgtc tttccactta
    24721 gtcgtgcgac tgaagatcca gatgaattag aagaagagcg ccgtctagcc tatgtaggta
    24781 tcacgcgtgc agagaaaatt ctctatctga ccaatgccaa ctcacgcttg ctttttggtc
    24841 gtaccaatta taaccgtccg actcgtttta ttaacgaaat cagttcagac ttgcttgagt
    24901 atcaaggtct ggctcgtcct gcaaatacaa gctttaaggc atcatatagc agtggtagta
    24961 tttcctttgg tcaaggtatg agtttggctc aggctcttca agaccgtaaa cgcggtgctg
    25021 ccccaaaatc aatccagtca agcggtcttc catttggtca atttacagct ggcgcaaaac
    25081 cagcatctag cgaggcaaat tggtccattg gtgatattgc tctccacaag aaatggggag
    25141 agggaaccgt tctggaagtt tcaggtagcg gtgctaggca ggaattgaaa atcaatttcc
    25201 cagaagtagg tttgaaaaaa cttttagcca gtgtggctcc aattgagaaa aaaatctaat
    25261 tttccatcct tctcacgaat aataaagtga ggaggatttt tatgtacagt atttcattcc
    25321 aagaagattc actattacca agagaaaggc tggccaagga aggagttgaa gcgcttagta
    25381 accaagagtt gctagctatt ttactcagga caggaacacg tcaagttagc gtttttgaaa
    25441 ttgcccaaaa agtcttgaac aatctttcaa gcctaacgga tttgaaaaaa atgaccctgc
    25501 aggaattgca gagtttgtct ggtattgggc gtgttaaggc catagaatta caagctatga
    25561 ttgaactggg gcatcgtatt cacaaacacg agactcttga aatggaaagt attctcagca
    25621 gtcaaaagtt ggccaagaag atgcagcagg aattagggga taaaaaacaa gagcacctgg
    25681 tggcactcta tctcaatact caaaatcaaa tcatccatca gcagaccatt tttatcggtt
    25741 ctgtaactcg tagtatcgct gaaccgcgag agattcttca ctatgcaatc aagcatatgg
    25801 cgacttctct tatcttggtc cacaatcatc cttcaggagc ggtagcgcct agccaaaatg
    25861 atgattatgt cactaaactt gttaaagaag cctgcgaact gatgggaatt gttctcttgg
    25921 accatttgat tgtctctcat tctaattact ttagttatcg tgaaaagaca gatttaatct
    25981 aaagttcatt aacgacatag tcaaagagtt tcttatcttt gggacgattt tcaaaaagaa
    26041 gttctggatg ccattggaca ccgagaaagg cgacatcatc cgtactcatg acagcctcaa
    26101 tgataccatc tttaggatca tgagccacaa cttttaaatt tggtgctaag tccttgatgc
    26161 tctggtggtg gaaggagttg atatgagaga tttctccata gatttcttgg agaacggtat
    26221 ctggttctgt caccaagcgt tgagttgtgt actcaacaga agaatcctgc caatggtctt
    26281 cgatatcttg gtacaaagtt ccacccatgg caacgttaaa gagttgggta ccacggcaga
    26341 cagagaaaat gggctttttt tgtttaatag cttccttgat gagggccagt tcgaagatat
    26401 ctctttgaag gtgatagtca tcgctatcaa tggttttggg ttcgccataa aattttggat
    26461 cgacattttg cccacctgtt aagatgagct tgtcaatcaa actgatatag tggcaggcca
    26521 tttcttgatc accaatcggt aggatgatgg gaatccctcc agcatcttta acgccttcaa
    26581 caaagccttt tgttgcgtag ctcatcatga tgtcatcatc tggatgagtt ttttcgtttc
    26641 ctgtaatccc aataactggt tttttcataa aatgattttc gctttctaat cctcttttcg
    26701 catgaagtag aggagggttt ggagttcact tgtcaaatcg acatactgaa cgaccacgtc
    26761 ttttggtaaa tgcagatgga ctggtgaaaa actgagaatt cctttcacac cagcatcaac
    26821 caagagatta gcaacctctt gtgacttgac gctgggaaca gttaggatag cagtcttcac
    26881 atcagcatcc ttgattttat ccttgatctg agaaatcccg taaatgggaa tcccgtcagg
    26941 agtttgggta ccgacttcag gatggtcgtc taggtcaaag gccatgataa tcttcatctt
    27001 gttacgttcg tggaagcggt agtggagaag ggcatggccc atatttccaa taccaaccag
    27061 catgacattg gtaatagagt tgtcattgag caaatcggca aaaaatgtca ttagtttttt
    27121 gacatcatag ccaaaaccac gacgaccaag ttcaccaaaa taggaaaaat cacgacgtac
    27181 ggtcgctgaa tcaataccga tagcctctgc aatttgctta gagttggcac gttcaatctt
    27241 ttctgcatga aatctcttaa aaattcgata gtagagagag agtctttttg ctgtagcttt
    27301 tggaatagca aactgtttat ctttcacaaa atcacaacct ttctattctt ctattttata
    27361 gaaacattgt gaaaaaatca acaaaaataa gaaaaaacta agaaaaatct tagttttgat
    27421 gtaaaaaatc tgcatgagat agaaaacggt agaggtctcc gaccagcccc tgataaactt
    27481 ttttgcccct aaaagtcaga gaagtcacat aaagtgtatc tggtaaggtt acacatcctg
    27541 acaaagtcaa catgagagcc tcatgatcct catacttgag agtacgctct acatgatagc
    27601 agtccttata ggtcagttca aacattttgg ctctatcttt ccgattttgt aaagacacca
    27661 cgttctacca agctatccat gaggaagtag aatttttcct gatgaatatg gtggtcttct
    27721 gatttgaaaa tatcaactag acgaaggcca aacttgtcag tgatattgat tttagcccct
    27781 gtaagttcct tgttaatgat gattttgagt tggaagcctt caccgctgtt tggcactttt
    27841 tccaaaaggc gagtcagttc atagttacca accttagttt caaaaaaggt gttatctttg
    27901 agggtgaatt ttttaacaga agggctaaga gtgtaatcgt aacgacaatt ttttaactga
    27961 atgatttttt caaatgccat atggctaacc tccgataatt tcttttaagg tttttgcgag
    28021 ggtttgtagg tcttcaacgg tattttgtgg cgacaaactg atgcgaaggg attccttcaa
    28081 gcgttctgaa tttgcgccat acatggcttc aagaacatgg ctggattgga caacgcctgc
    28141 agtacaggct gagccagtag agattgaaat tccagctaaa tctagccgaa ggagtaagag
    28201 gtcatttttc tgaccaggaa atccaatatt gagaacataa gggagatgat gttttcctct
    28261 attcaagtaa tactgaatgc cctccagctc tgccagaaag gcagtttcta gattttgtac
    28321 atgttgaaaa tgttcttctt gtttttctag gtcttctttt agggctgcaa ccatgcctac
    28381 gatggcaggc agattttcag ttcctgcacg ttttttctgt tcctggtctc cgccatgtag
    28441 ataggaatca aagtccatgc tagatgcgta gagaaaaccg attcccttag gaccatggaa
    28501 tttgtgggca gaagcagtga gaaaatcaat gcccaattct tctgaatgaa ttgggatttt
    28561 accaatagcc tgaactgcat caacatgata ggcagcaggg tgttgcttga gtatttggcc
    28621 aatttcagcg atgggcagta ggtttcctgt ctcattattg gcaaacatgg tagaaaccaa
    28681 aatcgtatcg tcacgtaaag ccttttgaat ttgctgggct gtgatttctt gattttttgg
    28741 ctggataatg gttgcttcaa acccaaagtg ttgaaccaag taatcaattg tttcaaggac
    28801 agcatggtgc tcgatggcag ttgtgatgat atgttttcct tgttcttggt gacgaagaca
    28861 gtagccaatg atggtagtat tattgccttc agtcccacca gaagtgaaaa agatatgttg
    28921 aggttttgtc cttagtaact gggctagttc ctgacgggct tctcgcaaga gtttgccagc
    28981 ttgacgacca tgaccatgaa tactagaagg atttccgtgg gtttcttgca taaccttggt
    29041 catagctgaa atagcaactg ctgacatagg agtcgttgca gcattgtcca aataaatcaa
    29101 agaatcacct tatttctttt tattgtaggc aaagagtggg ctgactggtt ttctttcgtg
    29161 aatacggacg atagcatcac caattaactc actagcagtg atgtagcata catttttagg
    29221 agttttttct tttgttgcta ctgaatcagt cacaagaatt tctttaatat tagtattgtc
    29281 aagaagttca gcagctccct cgacgaagag accgtggcta gaaacagcat aaatttctgt
    29341 agctccttca cgttcaacga ttttagaagc ttcagagaag gtacgtcctg tatttaaaat
    29401 atcatcaatc aagatagctt tcttaccttc aacatcacca ataatataac cttcgttacg
    29461 agttgcatcg tcttgagggt agtcgataat ggcgatagga gcatcaagat attcagccag
    29521 gctacgcgca cgtttgacac ctgaattttt agggctaacg acaacaacat ctgaaccaag
    29581 caatccttta tcgcagtaat gttttgcgaa taggggaaca gtgaaaagat tatccactgg
    29641 aatatcaaag aaaccttgaa cctgaacggc atgcaaatca agagtcagga tacgatcaac
    29701 tccagcctta accagcatat tggcaactag ttttgctgta agtggctcac gaggacaagc
    29761 aatgcggtct tgacgtgcat agccaaaata tggaaggaca acgttgatac tgtgggcact
    29821 tgcacgcaca caagcatcga ccatgattaa caattccatt aggtggttgt tgacagggaa
    29881 acttgttgat tggatgatgt aaacatcata accacggaca ctttcttcga tatttacttg
    29941 gatttctccg tctgaaaatt gacgtgatga tagttttcca agtgggacac caacagcttg
    30001 ggcaattttt tgtgcaatct cttggttaga gttgagtgcg aaaagtttca tgttttttct
    30061 atctgacatt atagaccgtc ctctgtaaac tttataaatc ctagttatat ttaccttaca
    30121 tatatgaact gggatttgtg tatttttatc ttttctattt taccaaaaaa tggagattat
    30181 ttcagctatt tttcatactt ttgacaaatc gaaccaattt tgaaggagct ttttggtagg
    30241 aaatctgatt tttctctaaa aattgtcgga aatcctgttt gccttgctca tgattttcca
    30301 cttcaagctc caattcgtaa tctgttatat caaagtatcg gctctgatcc agtgccatga
    30361 gaccaatagc tgttttcatt tcatagcgaa gcgttgttag acaaccaaga acctgccagt
    30421 tcttactttg gataccatgt ttcgccaatt catccagtac tagcccttga ggaagttctt
    30481 ccttactcag atagttctca gcatctttta gttgtaattt ttggttgtat tccatgtttc
    30541 caacactctg cgggactttg agtgtcaact cagcccagtc ttcaaaggtt cgaatgcgca
    30601 tagcgacttt cttttctcgc agttcaaaat caggcgtgtc gatgtagtaa tttgtttgaa
    30661 gaacaggagt gacacctgtg aactggtctt ttagacgatt gtattcatct tttttcaata
    30721 gtgttttcaa ttcaatttct aaatgtttca tttttcttac ctttttttat cgttgaaagc
    30781 ggatttatgg tataataagc attgtattta ttgtatatga atctggagaa aaaatcaaag
    30841 atatttttga cggataatat gagaacaagg gagaatatat gaccttagaa tgggaagaat
    30901 ttctagatcc ttacattcaa gctgttggtg agttaaagat taaacttcgt ggtattcgta
    30961 agcaatatcg taagcaaaat aagcattctc caattgagtt tgtgaccggt cgagttaagc
    31021 caattgagag catcaaagaa aaaatggctc gtcgtggcat tacttatgcg accttggaac
    31081 acgatttgca ggatattgct ggcttacgtg tgatggttca gtttgtagat gacgtcaagg
    31141 aagtagtgga tattttgcac aagcgtcagg atatgcgaat catacaggag cgagattaca
    31201 ttactcatag aaaagcatca ggctatcgtt cctatcatgt ggtagtagaa tatacggttg
    31261 ataccatcaa tggagctaag actattttgg cagaaattca aattcgtact ttggccatga
    31321 atttctgggc aacgatagaa cattctctca actacaagta ccaaggggat ttcccagatg
    31381 agattaagaa gcgactggaa attacagcta gaatcgccca tcagttggat gaagaaatgg
    31441 gtgaaattcg tgatgatatc caagaagccc aggcactttt tgatcctttg agtagaaaat
    31501 taaatgacgg tgtaggaaac agtgacgata cagatgaaga atacaggtaa acgaattgat
    31561 ctgatagcca atagaaaacc gcagagtcaa agggttttgt atgaattgcg agatcgtttg
    31621 aagagaaatc agtttatact caatgatacc aatccggata ttgtcatttc cattggcggg
    31681 gatggtatgc tcttgtcggc ctttcataag tacgaaaatc agcttgacaa ggtccgcttt
    31741 atcggtcttc atactggaca tttgggcttc tatacagatt atcgtgattt tgagttggac
    31801 aagctagtga ctaatttgca gctagatact ggggcaaggg tttcttaccc tgttctgaat
    31861 gtgaaggtct ttcttgaaaa tggtgaagtt aagattttca gagcactcaa cgaagccagc
    31921 atccgcaggt ctgatcgaac catggtggca gatattgtaa taaatggtgt tccctttgaa
    31981 cgttttcgtg gagacgggct aacagtttcg acaccgactg gtagtactgc ctataacaag
    32041 tctcttggcg gtgctgtttt acaccctacc attgaagctt tgcaattaac ggaaattgcc
    32101 agccttaata atcgtgtcta tcgaacactg ggctcttcca ttattgtgcc taagaaggat
    32161 aagattgaac ttattccaac aagaaacgat tatcatacta tttcggttga caatagcgtt
    32221 tattctttcc gtaatattga gcgtattgag tatcaaatcg accatcataa gattcacttt
    32281 gtcgcgactc ctagccatac cagtttctgg aaccgtgtta aggatgcctt tatcggtgag
    32341 gtggatgaat gaggtttgaa tttatcgcag atgaacatgt caaggttaag accttcttaa
    32401 aaaagcacga ggtttctaag ggattgctgg ccaagattaa gtttcgaggt ggagctattc
    32461 tggtcaataa tcaaccgcaa aatgcaacgt atctattgga cgttggagac tacgttacca
    32521 ttgacattcc cgctgagaaa ggctttgaaa ccttggaggc tattgagctt ccattagata
    32581 ttctctatga ggatgaccac tttctagtct tgaataaacc ctatggagtg gcttctattc
    32641 ctagtgtcaa tcactctaat accattgcca attttatcaa gggttactat gtcaagcaaa
    32701 attatgaaaa tcagcaggtt cacattgtta ccagactaga tagggacact tctggcttga
    32761 tgctctttgc caagcacggt tatgcccatg cacgattaga caagcagttg cagaagaaat
    32821 ctatcgagaa acgctacttt gctttggtta agggagatgg acatttggag ccagaagggg
    32881 aaattattgc tccgattgcg cgtgatgaag attccattat taccagacga gtggctaaag
    32941 gcggaaagta tgcccatact tcatacaaga ttgtagcttc ttatggaaat attcacttgg
    33001 tctatattca cctgcacact ggtcgaaccc atcaaatccg agtccatttt tctcatatcg
    33061 gttttccttt gctgggagat gatttgtatg gtggtagtct ggaagatggt attcaacgtc
    33121 aggctctgca ttgccattac ctatcctttt atcatccatt tttagagcaa gacttgcagt
    33181 tagaaagtcc cttgccggat gatttcagta accttattac ccagttatca actaatactc
    33241 tataaaaact gtctcagagt ataattatta tcttaaagga gaaaactcat ggaagttttt
    33301 gaaagtctca aagccaacct tgttggtaaa aatgctcgta tcgttctccc tgaaggggaa
    33361 gagcctcgta ttcttcaagc aacaaaacgc ttagtaaaag aaacagaagt gattcctgtt
    33421 ttgcttggaa atcctgaaaa aattaaaatt tatcttgaaa ttgaaggaat catggatggt
    33481 tatgaggtca tcgaccctca acattatcct caatttgaag aaatggtttc tgccttggtg
    33541 gagcgtcgca agggcaaaat gactgaagaa gatgtacgca aggttttggt tgaagatgtc
    33601 aactactttg gtgtgatgtt ggtttacttg ggcttggttg atggaatggt gtcaggagcg
    33661 attcactcaa cagcttcaac agttcgccca gctctacaaa tcatcaaaac tcgtccaaat
    33721 gtaactcgta cttcaggagc cttcctcatg gttcgtggta cggaacgtta cctatttgga
    33781 gactgtgcca ttaatatcaa tccagatgcg gaagccttgg ctgaaattgc catcaactca
    33841 gcaatcacag ctaagatgtt tggcatcgaa cctaaaattg ccatgttgag ctattctact
    33901 aaaggttcag ggtttggtga aagcgttgat aaggtcgttg aagcaactaa aattgctcac
    33961 gacttgcgtc ctgaccttga aatcgatggt gagttgcaat ttgatgcggc ctttgttccc
    34021 gaaactgcag ctctgaaagc tccgggaagc acagtagctg gtcaagcaaa tgtcttcatc
    34081 ttcccaggta tcgaggcagg aaatatcggt tacaagatgg ctgaacgcct gggtggcttt
    34141 gcggctgtag gacctgtttt gcaaggttta aacaagccag ttaatgatct ttctcgtgga
    34201 tgtaatgcag atgatgttta caagttgacc ctcatcacag cagctcaagc agttcatcaa
    34261 tagtgaaaac tataaagtga tatactatgc tatactgtag ttatgaaact atgtacgaaa
    34321 agcactgcca ttaattcctg agaactaaat tactgattgg tgtcaaaaag gaaaacttcc
    34381 aagcgatgat atcctgtcta tacacgacct atagaaatct gtaatataca tgtccgtaaa
    34441 acgataaatt ccctttttga ttttaaatga gtatgaaaag agaatttttt ggctctttgt
    34501 caactgtagt gggttgaagt cagctaagct cgagaaagga caaattttgt cctttctttt
    34561 ttgatattca gagcgataaa aatccgtttt ttgaagtttt caaagtttcg aaaaccaaag
    34621 gcattgcgtt tgataagttt gatgagatta ttggtcgctt ccaatttggc gtttgaatag
    34681 ggtagttgaa gggcgttgac gattttctct ttgtccttta gaaaggtttt aaagacagtc
    34741 tgaaaaagag gatgaacctg ctttagattg tcctcaataa gcccgaaaaa tttctctggt
    34801 tccttattct ggaagtgaaa cagcaagagc tgatagtggt gtttcaagtc ttgtgaatag
    34861 ctcaaaagct tgtttaaaat ctctttattg gttaagtgca tacgaaaagt aggacgataa
    34921 aaacgtttat cactcagttt acggctatcc tgttgaatga gtttccagta gcgcttgata
    34981 gccttgtatt catgggattt tcgatgaaac tgattcatga tttggacatg cacacgactc
    35041 atagcacggc taagatgttg tacaatgtga aagcgatcaa gaacgatttt agcattcggg
    35101 agtgaaacag tctgggagac tgtttcagcc tgagcctaga aatttgaaag cgaagctgtt
    35161 tagccaagcc atagtaaggg ctaaacatat ccatagtaat gattttgacg cgacatcgga
    35221 cggctctatc gtagcgaaga aagtgatttc gaatgatagc ttgtgttctt ccctcaagaa
    35281 cagtgatgat attgagcttg tcaaaatctt gcgcaataaa gctcatctcc atctcccgat
    35341 tgaaacagtc actccccgga ctgtttcaac gtcccaagac ataatctcag gcagatttct
    35401 aaaattacac tcaaagtgga agtcattgag cttacgaata acagttgaag ttgagatgaa
    35461 aagctgatgg gcaatatcag tcatagaaat cttttcaatt aacttttgag caatcttttg
    35521 gttgatgata cgagggattt ggtgattttt cttgacgata gaagtttcag cgaccatcat
    35581 ttttgaacag tgatagcact tgaatcgacg ctttctaagg agaattctag taggcatacc
    35641 agtcgtttca agataaggaa ttttagaagg tttttgaaag tcatatt
//
LOCUS       DAVKLX010000016        34667 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKLX010000016 DAVKLX010000000
VERSION     DAVKLX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.016"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(203..1300)
                     /locus_tag="VW325_000874"
     CDS             complement(203..1300)
                     /locus_tag="VW325_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9734305.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARESDLPEGIHVLGFTEKGRQHLKYLKGQVSLVSRIGKEPWDV
                     MTQKADQIYQLGNPSIAEQNFGRVPIRIETN"
     gene            complement(1310..2056)
                     /locus_tag="VW325_000875"
     CDS             complement(1310..2056)
                     /locus_tag="VW325_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9734306.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(2159..2512)
                     /gene="rsfS"
                     /locus_tag="VW325_000876"
     CDS             complement(2159..2512)
                     /gene="rsfS"
                     /locus_tag="VW325_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9734307.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(2525..3025)
                     /locus_tag="VW325_000877"
     CDS             complement(2525..3025)
                     /locus_tag="VW325_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9734308.1"
                     /translation="MKMAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKIAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(3026..3619)
                     /gene="yqeK"
                     /locus_tag="VW325_000878"
     CDS             complement(3026..3619)
                     /gene="yqeK"
                     /locus_tag="VW325_000878"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9734309.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPGLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRSFPGVDVAREVASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(3619..4248)
                     /locus_tag="VW325_000879"
     CDS             complement(3619..4248)
                     /locus_tag="VW325_000879"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9734310.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLVPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(4300..4611)
                     /gene="yhbY"
                     /locus_tag="VW325_000880"
     CDS             complement(4300..4611)
                     /gene="yhbY"
                     /locus_tag="VW325_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9734311.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(4825..5931)
                     /gene="yqeH"
                     /locus_tag="VW325_000881"
     CDS             complement(4825..5931)
                     /gene="yqeH"
                     /locus_tag="VW325_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9734312.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAYEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            complement(5934..6524)
                     /locus_tag="VW325_000882"
     CDS             complement(5934..6524)
                     /locus_tag="VW325_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9734313.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(6476..7384)
                     /locus_tag="VW325_000883"
     CDS             complement(6476..7384)
                     /locus_tag="VW325_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9734314.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFV
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(7441..8001)
                     /locus_tag="VW325_000884"
     CDS             complement(7441..8001)
                     /locus_tag="VW325_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9734315.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            8140..9355
                     /gene="sstT"
                     /locus_tag="VW325_000885"
                     /pseudo
     CDS             8140..9355
                     /gene="sstT"
                     /locus_tag="VW325_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            9423..10376
                     /locus_tag="VW325_000886"
     CDS             9423..10376
                     /locus_tag="VW325_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9734316.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(10474..10551)
                     /locus_tag="VW325_000887"
                     /pseudo
     CDS             complement(10474..10551)
                     /locus_tag="VW325_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(10612..10836)
                     /locus_tag="VW325_000888"
     CDS             complement(10612..10836)
                     /locus_tag="VW325_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEU9734317.1"
                     /translation="MIEILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(10841..11020)
                     /gene="asp4"
                     /locus_tag="VW325_000889"
     CDS             complement(10841..11020)
                     /gene="asp4"
                     /locus_tag="VW325_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEU9734318.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(11022..12359)
                     /gene="gtfB"
                     /locus_tag="VW325_000890"
     CDS             complement(11022..12359)
                     /gene="gtfB"
                     /locus_tag="VW325_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEU9734319.1"
                     /translation="MIELYDRYSHESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIAGALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(12352..13863)
                     /gene="gtfA"
                     /locus_tag="VW325_000891"
     CDS             complement(12352..13863)
                     /gene="gtfA"
                     /locus_tag="VW325_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEU9734320.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEVQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLRDIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(13875..16247)
                     /gene="secA2"
                     /locus_tag="VW325_000892"
     CDS             complement(13875..16247)
                     /gene="secA2"
                     /locus_tag="VW325_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEU9734321.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(16240..16680)
                     /gene="asp3"
                     /locus_tag="VW325_000893"
     CDS             complement(16240..16680)
                     /gene="asp3"
                     /locus_tag="VW325_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEU9734322.1"
                     /translation="MIITQRQSIHWGEVGGTYMYGTTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKVKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(16677..18212)
                     /gene="asp2"
                     /locus_tag="VW325_000894"
     CDS             complement(16677..18212)
                     /gene="asp2"
                     /locus_tag="VW325_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEU9734323.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKALREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(18196..19776)
                     /gene="asp1"
                     /locus_tag="VW325_000895"
     CDS             complement(18196..19776)
                     /gene="asp1"
                     /locus_tag="VW325_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEU9734324.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFGPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(19786..21003)
                     /gene="secY2"
                     /locus_tag="VW325_000896"
     CDS             complement(19786..21003)
                     /gene="secY2"
                     /locus_tag="VW325_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEU9734325.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     RMYLTLLIAVIQSLAVSLSLPVQSSYSVILVALMNTLLLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSIVLNIPQDVIETFKLVYIPTGIVVLLVFMTIIFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMVGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVIMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYRPLI"
     gene            complement(21060..21737)
                     /locus_tag="VW325_000897"
     CDS             complement(21060..21737)
                     /locus_tag="VW325_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9734326.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGKDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(21722..21967)
                     /locus_tag="VW325_000898"
     CDS             complement(21722..21967)
                     /locus_tag="VW325_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9734327.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGGQHKLKIWGLV"
     gene            complement(21967..23163)
                     /locus_tag="VW325_000899"
     CDS             complement(21967..23163)
                     /locus_tag="VW325_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU9734328.1"
                     /translation="MRKSIVLAADNVYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNSGVLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(23172..24392)
                     /locus_tag="VW325_000900"
     CDS             complement(23172..24392)
                     /locus_tag="VW325_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEU9734329.1"
                     /translation="MLQDKKSVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(24417..25301)
                     /locus_tag="VW325_000901"
     CDS             complement(24417..25301)
                     /locus_tag="VW325_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9734330.1"
                     /translation="MKDLISVIVPVYNVEPFISSCLDSLSKQIYQNFEVLLVNDGSTD
                     NSGAICREYADRDSRFHYFEKENAGVADARNFGIERSKGDYITFVDSDDWVTEEYLSI
                     LIETLKEQHSEIVVSTYSTYNESDGLFYIHVFDSDYYVKNYNSKLLMEELPLLERYDM
                     SFLTSWGILFKRELFQEVQFPFGRVCEDIGTNYKLFMQVEKVTYINKVLYWYRVGKEG
                     LSNSYSPKMMRDDCDFRLERIAVLALKGYDVSKYLDQMKFYLKYRHDIAIQRELKENV
                     ETRHLEMLDYLLNGNKYN"
     gene            complement(25298..27751)
                     /locus_tag="VW325_000902"
     CDS             complement(25298..27751)
                     /locus_tag="VW325_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEU9734331.1"
                     /translation="MIKTIVLAGNRNYTRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIASMLGSEIIDVKLPEQTVFQDWKKQAHISSIAYARYFIPDYIQEDTVLYLD
                     CDLLINDKLDSLFEQDVREHYIAAIRDANGQGFNTGVLLINNEKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWNQILQHHLGEFELISPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEVNLMWGLPIPYQNHDLELANQLKHIVGLESDEKLVVCLPDAFDDRFVFTW
                     WATPFWKEHMNVYMDFYKELCKGSWYGSTFISRPYIDYEDKSKAKGQFEKLKSIWENR
                     DILIVEGITSRSGVGNDLFDKVKSVKRIICPSHNAYSVVDNIQEEIMKHAEGRLILCM
                     LGPTAKVLAYHLSRKGYQVLDIGHIDSEYEWMKMGAKTKVKFSHKHTAEYNFDQDIQF
                     IEDETYNSQILVDLSKREV"
     gene            complement(27761..28771)
                     /locus_tag="VW325_000903"
     CDS             complement(27761..28771)
                     /locus_tag="VW325_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9734332.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKRTFFAGSLERFPDLQNWSQDTPLRVFSNKGEAGSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSNLSTAKFIV
                     DQGLGFVANSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            complement(28800..29675)
                     /locus_tag="VW325_000904"
     CDS             complement(28800..29675)
                     /locus_tag="VW325_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9734333.1"
                     /translation="MENELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVVAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            complement(29680..30894)
                     /locus_tag="VW325_000905"
     CDS             complement(29680..30894)
                     /locus_tag="VW325_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU9734334.1"
                     /translation="MYRKVIALGADIHYLDKVETVIKSVSVHNHEVKFYVFNDDLPSE
                     WFLLMRNRLKVIGSEIINVKKADHNLRDFHLPNAILSYATFFRYFIADEVLEDRVLYL
                     DSDIIVNANLDELFFLDLQGYAIAAVQDFDQDGWLTTFNSGMLLIDAKKWREKKSTQN
                     LLELTAQHHEHVYGDQGVLNIYFGDQWLHLDKEYNFMVGLDQFLHLKGNADWYQSNYY
                     GNYEPKIVHYTTEFKPWTHLTLTRFRKLWWFYYGLNWNDVLLSSDIVKRSFKELVGVP
                     LYHTCIFTNSAAMESLEYLLSELPEVHFTILAHTNFAPSVVDLQRYLNLSLFPNFNSF
                     NMKETLDKMDFYLDINHEGEIVNIIEEVCRRNKPIFSFDNTSHDSKGRSRIFSATEPD
                     KMVDAIRKFLGE"
     gene            complement(31251..32129)
                     /locus_tag="VW325_000906"
     CDS             complement(31251..32129)
                     /locus_tag="VW325_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEU9734335.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLSNICFHIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVACRNFENFHVSSGKLILNNGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLFVKMRV"
     gene            complement(32126..33526)
                     /locus_tag="VW325_000907"
     CDS             complement(32126..33526)
                     /locus_tag="VW325_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9734336.1"
                     /translation="MHALVDVMSERITLLANMGYPLEKHLAIYRQMLEFSLANGQASG
                     LSDTATYKEFEMKKRLLNQLTVQESSEKKAIVLAANYAYVDQVLTTIRSICYHNRSLR
                     FYLIHSDFPNEWIKQLNKRLEKFDSEIINCRVTSEQISCYKSDISYTVFLRYFIADFV
                     QEDKALYLDCDLVVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNA
                     FWKKENMTQKLIDLTNEWHDKVDQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQ
                     LPEGQDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIY
                     PIKEPFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNG
                     IHYLVDVDNELVETSQVLLDINHGEKTEEILDQFANLGKPILSFENTKTYEVGQEAYA
                     VDQVQAMIEKLREISK"
     gene            complement(33573..>34667)
                     /locus_tag="VW325_000908"
     CDS             complement(33573..>34667)
                     /locus_tag="VW325_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9734337.1"
                     /translation="ASASTSASASASTSASASASTSASASALTSASESASTSASASAS
                     TSASASASTSASASASTSASASASTSASASASTSASTSASTSASASASTSASASASTS
                     ASASASTSASASASTSASASASTSASASASTSASESASTSASASASTSASVSASTSAS
                     ASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASAS
                     ASTSASASASTSASESASTSASASASTSASASASTSASESASTSASASASTSASASAS
                     TSASASASTSASESASTSASASASTSASASASESASTSASASTSAGKSRQQLPNTGTE
                     ASKSSVLLGALAAVTGLGLFAKRRKRDDEE"
BASE COUNT        10531 a         7606 c         5868 g        10662 t
ORIGIN      
        1 ttttaatata accagttctc ttcattatac cacaaaataa caagcataaa gcggattcaa
       61 agataaagat taaggttcat atactaattt tctttattga ctttttgtct tctgtattag
      121 ggagtttatc attttccaaa aactttatct tcaatttgtt agctaaaatt tctacaataa
      181 aaaagcctac ctttcagtag acttagtttg tttctattct aatcggcact ctgccaaaat
      241 tctgttctgc tatacttgga tttcccaatt ggtaaatctg gtctgccttt tgagtcatga
      301 catcccaagg ttccttgcca attctgctga ctagactgac ctgccctttc agatatttaa
      361 gatgttgcct gcctttttcg gtaaagccaa ggacatgaat cccttctggc aagtcacttt
      421 ctctagcctg caccaagata taggtcaaga ggcgtctgac acgcgccttg gtgtaacgtt
      481 tggtggtcac taactcgacc aattcttcca cagactgggc tgttttaata gcttctttaa
      541 tgcgcacagc catttcttga ttgacctgat agatggtggt taggtcggga tttgacaaga
      601 tttgatagcg gagcaaggga aaatagtctt cccagatcac cttactggcc tgctcaaata
      661 gggcaacaga aggcataaaa cgttctaaga aatcttggtc cttctgatgt tgacggaggg
      721 ctgtcgccga ggcaaagtcc acatctttat tcacagaatg gtaacctgcc ccctgacgct
      781 gaatcggatg cagcttgata tttcgtcctg caaccgcctt tgcataggcc agagcaagaa
      841 catgattggg ggtattacct gaaaaatcaa gaccagcaaa ttccttccac atagcttggg
      901 ttttctgggg ataggaaagg gaatcaggca gattttccac aaatttctcc atctcagcac
      961 ctttctctgt gtataagtca gcgattttct gataatctcg aacttcctct gtcccaaaaa
     1021 ctagactatc aatgcccaag cgatccaaga tatccacagc tccttgacca aagaaatcag
     1081 ctgcctgaac actgactaaa aagggcaatt ccactaccaa gtctgcgcca ttttccagcg
     1141 ccatctgggc ccttgtccac ttatccacaa tagcaggctc tccacgctgc atgaaattcc
     1201 ctgacatggc aacaattttc agtccctctg cctgatccag caggtattta tggccattat
     1261 gaaaaggatt gaactccgcg ataatacctg tgatggtcat gctaatctcc tacttctgcg
     1321 ccacaaaaaa ccaacgggtg ctagtttctg ttggctcctt atcctcaaag tctgcataga
     1381 gtttgaagga cttgaatcca gcctgttcca gcaagatatc ataggtcaag atctcatagg
     1441 tcctctcctc atgtacttca tcgtggcgac taaaggaacc gtcagcctcc ttgataaaga
     1501 aagtcagctc atgcacgatg gagtgaggaa cttcgtcctc atatgtatcc caaagcatgg
     1561 caaaatcttc cgcattttca tggtaggaat agccagggaa tacttcatct gtctggtagg
     1621 tcgagtgcac gtcaaagatg aaaactccct cttcattcag cgcattgtac acgtccttaa
     1681 agacgtcccc tacttccacc tcatcctgca tatagcagat agagtccgaa taacacgtga
     1741 caaaatcgta tttccctgcc ttggacaaat ttagcatatt accttctata aaagcaatct
     1801 tttgcttggc tgaagttgct ctcttctccg caatcttcaa catatccgca ctcaagtcaa
     1861 gtccagtcac atcaaaacca gcctgagaga agcgcactga ttgaattcct gtcccacaag
     1921 ccaattccaa gagtttcttt ctctccttgg tcttaggcaa atgacgcaga gaaaaattcg
     1981 tccatttgtc gtataaacta tcgtccatga ccgcatcata gacagccgca aaggtttcat
     2041 aagtcgccat aatcatgata ctaagagctc catggtaaac accactagcc ctttaccttt
     2101 ctatcaattt ttctaaaata agcaaagaaa cccagttgac tacaactgag ttcatcttct
     2161 aagcaagagc ttctgaaata tctactgaat tcgcctcatg ccatagcttc tctagattat
     2221 aatgggcacg catttcttct gaaaagatat gcacgacaac agcaccgagg tccagtaaga
     2281 cccagcctcc agctgcatcg ccttcgacat ggctaccttt aaagcctgct tgagctactt
     2341 tttcacggat attagctgcg atagcgtcca gctgacggct attcattgag ctagtgatga
     2401 caaagtagtc cgtcacacta gtcaaatctt gtacatcaag tgcgaggata tcctccgcac
     2461 gtttctcatc agccgctttc acgactagtt ctagtaattc tttttcgttc atttaatcct
     2521 ctttttagaa aatatgtttg taatctagga catctgcaaa gcgctcctcc caaatgtcct
     2581 cataaaattc attaatcgtt tctgctggaa tgtcatcccc atcaaaggtt gtgacagcac
     2641 cttctggaac aataagctga tagccatatt caaaggcaat cttgacagag gtatccacac
     2701 aatattctgt ctgcatacca cataaaacta atttttcaat cccctgctta tccaagtatt
     2761 cttttaagcc agtttctttg aaaatactgt tatatttctt ctgaaagacc ttttcagcag
     2821 gttttcgatt taaaagcgca gataactgcc aatcttctga tgtttgagct tcagagttct
     2881 caatatgttg aacatagata atttcaatat tcttgcttct agcctggttt tgtaaataag
     2941 aaattttttc caatagactt tttgtctgga acccagtttc tactaaaaca ttctgaacat
     3001 caatgattat aaaagccatc ttcatttagt tctctttcaa atagtgcaca aaggcgttat
     3061 aggtttcaag ggtttgggga tagatgggga atccctgatg agctagatac tccacggtac
     3121 gagctgtttc gtaggccacc gccttattga gcgatagact tgcaacctca cgcgccacat
     3181 ctactccagg aaaggatcga ttgtgctcga tatagtctgc tacatagaca accttatcta
     3241 ggtctgtcat ctgaccagca ccgactgtat ggacttcaat ggctcgcagg atttctgaat
     3301 catgcaaatc caaatcttcc tgaattttgt aaattccgac cataccatgc cagacattat
     3361 tgccccagtt tttgaggcca gggtctagct ggtaacggtc aatcaagtct agaaattcct
     3421 gatctgacaa ctttttagca tagtcatgaa gaagacctgc tagacttgct ttctcaacat
     3481 ctacaccaaa gcgctgagct aattccatag cggcacgctc cacacctaga caatgggtta
     3541 gacgtttctc aggtagaagc tctgccattt tttccaacaa ggcctcacgg gagcagttga
     3601 tatagtcttg ataggacatc agtagagccc ctccttctcg atgtagtcta gcactggctg
     3661 aggtaggaga aagttgggtt tccgaccttg ggcaatgaag tcccgcacca tgctggacga
     3721 gatatccatg agcggtacgt ccacccagat aactggatag gaagtccctg ccttgtagcg
     3781 tggacgctga acccccacaa actgaaccat gtcaaccagt tcatcaattc ggtaccactt
     3841 aggcagatag tcaaccatgt cggcgccgat gataaagtaa taatccgtat ctggatgttg
     3901 ctctgtcaag attttcatgg tgtcgtaggt gtaggaaata cccttgcgct ccaactcaat
     3961 ggtttcaatg actaggccgt caatcccctc aattgccaac tcaagcatct tgagacgatg
     4021 gtgttcaggg atggtttcct ttttatcaac gtgaggaggt tggtattcag gcacgagaag
     4081 aacttgatcc agtcccaact gttgccgtac ttgatccgca acaatgagat gggcattgtg
     4141 aacagggtta aaattccccc ctaaaatccc aacttgtttg cgttttttct ccttgatttc
     4201 tggctccaac tctaccttgg taaagggagt caatagttcg attgtcatag gctagtcccc
     4261 tttatttctc tataaaaaca gttatttgga gtttcggtct tagatttctt tgactttctt
     4321 agaaatcttg cgattttctt tcttgctaga ttgtttaaac aaaatcaaga tgcgtcctat
     4381 tttttggact gtatccacac cgatttcttc ttccaaaatt tcagctactt cgtggatgtt
     4441 ttcatctgtg ttttgtaaga gagtaacctt gattaattca cgcgcatcaa gagcttgacg
     4501 gacgctggtt ttgatttggt cgttgagtcc atttttcccg atttggatga taggtttgag
     4561 ggtgtgtgcc tggctgttga ggaaggcacg ttgttttgat gttaatgaca taattttctt
     4621 ccttgttttt tgatagttat tttaggtggt gagggacggt cggaactaat tttccaaaga
     4681 tttcatcttt gaaaaatgga tttcctgacc tcacaattga gccttggtct caattgtgcc
     4741 ccttgccaat ctacagattg gcaatcacac cacggcaata actatcgcta tatgagctca
     4801 ctttgttcgc tcagcgattt tgttttaaat aattgctttt cgtgtaacga cggcgacgcc
     4861 ttctggtgcc cagacggcga cttttgctgt gcctgttaca cgaatccagc ctaggcctga
     4921 gatgactagg tctgtcttat ctttaatggt aaagacatgc tggactagcc ttgggaaatc
     4981 ttctttttcc ttgctatttg gtggtgtcag aagagttccc aggtgcttat cgtagaaagc
     5041 actagctcct tcaagcttgc tacgatggag tttgagttca ttatcaaaga aagcagtaaa
     5101 tccttgcttt tctcctgcta taaagtcaaa gcgtcccaaa ccacctaaaa atagggtttg
     5161 ctcaggatta agctgatagg tcttaggctt gatttccttt ttaggactga catacttgag
     5221 gtttttggcc gtcaagtagt gagccatctg gtggcggtgg ataattcccg gcgtatcgta
     5281 aatataagat ccgtcgtcaa gcggaatctc tattttgtcc aaggttgtcc ctgggaagcg
     5341 tgaagtagtg atgacattct gatcacccgt gatttcttgg ataatagcat tgattagagt
     5401 tgattttcca acgttggtca caccgaccac atagacatcg cggcccttac ggtagtgttc
     5461 aatcttgtca atgacttcct taatggcatg tttattttgt gctgaagtta ggaccacatc
     5521 gactggacga agaccttctt cataggcacg tttcatgagc cactggctaa tcttaccaga
     5581 cttaactgac ttaggaagga tatctttttt atttcctacc aagaggacat cattgcccga
     5641 gacgaaacgt ggtaaacctg ggatgacaga tccattaaaa tcaaagatat caatgacatt
     5701 gaccactaaa gcatcactgt ctcccacctc gtgcaagagc ttgaggaaat catcgttcgt
     5761 caactggaca tctgtgattt cattgtagtg gcggagacgg aaacagcgtt ggcaatagac
     5821 ttcgccagtc tccaaacctt tttcaagtgc cgactggggg gtaaaaccaa gaccagcttt
     5881 atctgtcgtc tgaatggttg ctccacaacc aatacagaga atttcttcca tagttaaatt
     5941 ccttttttat atgtaatcgg tccgtacttt tcagtgattt ttctcataac acgacgctca
     6001 cgagttcggt taatctgcgt tttgattgag tcatgttgga ccaagggttt gactaaaatt
     6061 gaccgaatcc ctgcacggtg ggctgctcgt atatctgtca tgagctggtc gccaaccatg
     6121 actacttcct ttttgtcata gtggaattcc ttcatggcac ggtcaatacc aaatgtgaag
     6181 ggcttcaagg cccagtaaac gtaatcaatc ccaaatttct caactgctcg ttgaacgcgt
     6241 tttttggtgt tatttgacac tacgataatg ccaatacccg cgtcccgaag gtcatgtagc
     6301 cattgcttca tctctggcgt tccatcaggg ttgttccaag caatgagggt attatccaaa
     6361 tcgaccaaaa cagccttgat tccctgcgcc tgcaggcttg ggactgtcag atcatagact
     6421 gcttccacag caaaatctgg tatataattt tcaatcgcca ttctggctcc ttttcttaac
     6481 tttttttcgc aattttcctt agtaacaagg ataaaacaat ccacaaacct gcactagcca
     6541 aactgatata gagccaagca tggggctcat ctgttaaagg taagggaaca ttcattccga
     6601 aaaagcctgt cacgactgcc aaaacagcta gcaagactga aatgatagtc aaggttgtca
     6661 aattgtcatt cagattattg tttagaatat tgttgtaaga ggctgaaagc tgctgtaaaa
     6721 tctgagagat taggtctgtc atggatacca gctgatgagc ctcaatcatg gcatcatcaa
     6781 actgttctct ctcaatctca tcaaaactac gatacaaggc atgaccttga atatgctcta
     6841 acaaaatccg attttgtttg gcagctgccg tcagataaac cataccagtc tccaaatcag
     6901 acaggacaaa gaggtttttc ttggtagttc gctggcgcaa gaggtcattg acctcatccc
     6961 tactcttgtc catctgctca atgacaggat agtaggcatt gctgataatt tccagactgg
     7021 caaagagaaa cttataaatc gaaagcgtgt catggctctc cagataacga gtcatctgtt
     7081 caatgacata ggcgttcttg gtattactaa tggtaatcag gcgacgatgc tcgacaataa
     7141 aggtcatggg aaaagtctca tagtaggcct tgtccttttt tacatctagg acattataga
     7201 taaaggtaac cgtcccactt tcacggtggt agtccatgtg ggcgcgctcg tttctatcca
     7261 gtgcgtattc aatggtttcc ttgtccaaac cgtaaatttc agaaaggtct tctagtttat
     7321 tcaactttcc taggtctagg tctatccagg tacaaccatt gcccaactgc ttttctaaaa
     7381 ccatcttttc tccttattaa actccctcta ttgtaccaca aattacttaa aataacaggt
     7441 ctagtctgtg cgtgagaaat tgctaacaac tgtgatattt cggttaggga caaagtgaag
     7501 ggcttgctcc tcgctacgaa gctgtgtcgt acgaattccc acactgacaa ccttacccga
     7561 tacagtaatc ggtccatttg tcagaacgac ctcatctccc acatccagtt gacgttcaaa
     7621 gaggatgaaa aagccattga tgacatcaga cagaaagcct tgggctccca taccaatcgc
     7681 taccccagca ataccagctc cagccagcaa actagaaact ggcaaaccta aaatcgacaa
     7741 aatgcagtag agtaaaaaga aatatagcgt ataattaaac acattttcta gtaaacgtga
     7801 gatggttttt tggcgtccga catcatgacg agacattttt agagaaggtt tgacaattct
     7861 ctgcaccatg gtatgaagca tttttttagc aatataaaat acaatcaaaa gcagtaaaag
     7921 agaaatgacc ttggttagaa tattctcgat aatggttgtc acatctagct tttcaatata
     7981 agcctgaata aatttttgca tataaaactc ctttatttca ttataccata ttttcatcag
     8041 tctcattctc cataaatatt caaaaaatag acagaaaatt cttgattttg tctatcattt
     8101 atactataat cataatcact acacaaaaag gagattatta tgaaaagaat cattcatgct
     8161 tggaataagg caagccttgt caagcgtatc ttgattggta tgctttgtgg ggaatcctag
     8221 gactgaccct tcctaatctc tcagggattg ggctactcga ggatttattc gttggaggtc
     8281 taaaagctgt tgctcctatc ctagtctttg ctctcgttgc caatgccctt tcccaacatc
     8341 aaaagggaca agatagcaat atgaaaactg ttgttttctt gtatatccta tgatagggac
     8401 tttcgccgct gctcttgtag ctgtactagc aagtttcatc gtccctattg aaattaccct
     8461 aaatagtgcc aatactgaaa ttgcaccacc agatgggatt gggcaggtcc tcagcaacct
     8521 cttgctcaaa ctggttgaca acccagtcaa cgccctgctt actgctaact atattggaat
     8581 cttatcttgg gcagtcattt ttggaatcgc tatgagagaa gccagtaaaa atagtaaaga
     8641 attgctaaaa actatcgctg acgtgacttc taaaattgtc gaatggatca tcaatctggc
     8701 tccatttgga atccttggtc ttgtttttaa aaccatttct gacaagggag tcggaagcct
     8761 tgccaactac ggtattttat tggttccatt agtaacgact atgctttttg ttgcccctgt
     8821 ggtcaaccct ttgattgcct tcttctttat gagtatgaga cgcaatcctt accctctagt
     8881 ttggaactgc ctccgtgtca gcggtgtgac agcctttttc actcgtagtt ctgcgactaa
     8941 cattcctgtc aacatgaaac tctgccatga ccttggactc aacccagata cctattctgt
     9001 ttctatccca ctcggttcta ctatcaatat ggctggagta gcgattacca ttaacgtttt
     9061 gacccttgtt acagttaaca ctcttggaat tcctgttgac tttgccacag cctttgtcct
     9121 cagtgtggta gcagctatct cagcctgtgg tgcttcaggt attgccggag gttccctcct
     9181 tcttatccca gttgcttgta gccttttcgg tatttctaac gatattgcca tacaagttgt
     9241 tggggttggt tttgtgattg gtgtcatcca agactcatgt gaaacagccc ttaactcttc
     9301 tacagatgtc ctctttaccg ccgttgccga atacgcagca acccgtaaaa aataactcat
     9361 caaggcaagc ctgctcatgt cttgtctttt acgcttttat tctaacttat taggaaattc
     9421 ttatgtctat tagccaacgt acgaccaagc tcatcttagc tacctgtctt gcctgcctgc
     9481 ttgcttattt tctcaatctt tcgtcagcag tttcggctgg aattatcgct ctcttgagcc
     9541 tatctgatac gcgtagaagt actttaaaac tggctcgcaa tcgtcttttt tctatgcttc
     9601 tagctctggc tatcggtgtt ctagcttttc acttgagcgg atttcatatc tggagtctcg
     9661 gcctctatct ggccttctac gttcctttag cctacaagat gggctgggaa attggcatca
     9721 caccaagcac ggttttggtt agccatctct tggttcaaga gtcaacctct ccagacctcc
     9781 tagtcaacga attccttctc tttgctattg gtacaggatt tgccttgctt gttaatctct
     9841 atatgccttc acgagaagag gaaatccagc actaccacac gctggtggaa gaaaagttaa
     9901 aagatatcct ccagcgcttc aaatactatt tatccagagg agacggacgc aaccgagcac
     9961 agctggtagc agaattagac acgcttttga aagaagccct cagactggtc tatttggatc
    10021 actctgacca cctctttcac cagaccgact accatatcca ctactttgag atgagacaac
    10081 gacaaagtcg tatcctgaga aacatggccc aacagattaa cacttgtcac cttgccgcca
    10141 gtgaaagcct gatcttagcg caactctttt caaaaattgc aggtcaactg agccagacca
    10201 atcctgcttc tgatttgcta gatgaaattg aacgttatct ggaagtcttc cggaaccgca
    10261 gtctgcccaa gacaagagaa gaatttgaaa cccgcgccac ccttcttcaa ctcctacgtg
    10321 aagccaaaac cttcatccaa gtaaaagttg atttttacca aaaatataga cagtaatact
    10381 cttcaaacta cgtcagcttc accttgccgt atatatgtta ctgacttcgt cagttctatc
    10441 cacaacctca aaacagtgtt ttgagctgac ttcgtcagtt ctatctacaa cctcaaaacg
    10501 gtgttttgag cagcctgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa
    10561 aaaagaaaac ttcagaccaa tccacaaaag gtgaatatct gaagttttct tttatttata
    10621 agtaatcacc ataaaggtta atagtagaac aaataaagcc aaactcacca ataaagtgag
    10681 caccttgacc aaggtgttgc tctgccagta gcttggttta ccaatattac tagtggcatc
    10741 catggaaaat agttgatttt gacggggttg tatagttacc aatacaatca aagcaagaga
    10801 taggataata gctaaaacaa ttagtatttc aatcatagat ctacctcaaa attcccagca
    10861 aagtaaagag caaaccaatc agaatcatca aacccagtaa aagtgaaaaa gtcttactaa
    10921 tcttttcccc tcgggtttct ttttccttct tgatgggttt ttgtttcaac tcttccatgc
    10981 ggccttcaac gtctttgtaa aataaatctt cctctgacat gttagcctcc taaaacagtt
    11041 tgcatggttt cctgatatct caccaagtca acataatttg catgttggcc ttgtttgcca
    11101 agtgcctgac gcatttgatc aacatctgaa agggccaatt taatggtctc aaccaaagca
    11161 gcaacttcac tactttcaaa tagatggtct ggagcgatat aaagtctatt gtgcaccgtc
    11221 tgattaaagc caagaatcaa gagattgtgc tcaaaggcct gacgcactgc ctgtagtaac
    11281 tcattactgt ggtttatatc caagtaaata tccgacagtt gatacagctc ctgaatcttc
    11341 tgtggactag cattctggta aaggaccaca ttaggatagc gaagcatgtc taagagctta
    11401 gaagacatct ctgtcaccgc tgcaatacgg aaagtgacat caggcaaggc tcctgcgatt
    11461 gcttctactt gctcaatctg atctgaattg gtcaagatta aggcatctcg tcttaggaaa
    11521 ttatcacgtt tgaactggta atgataaccc aagtgcacaa actgatcatg gtatttctcg
    11581 tcagttaact ctaaagcgcg ctcataagtc gccttatttg gaatgatgat cttcttagta
    11641 cgcacattat cactttccaa aatcaactgc atattacctg gaatggcatc atagagaggt
    11701 tcctgccata ccaagacatc cgagccagat ttatctggat gatggaagga aaccaagaaa
    11761 ggagtcgcta gagtattaaa gataagctga ctggtatcta tttccaaatc ttgcaaaaag
    11821 aaggtgataa attcaacttt atttgcaaag taacgcatgg actgaccagg caaagtcaat
    11881 aagatatcac ccgtcacatg gttttccagt aaaacttgtt ggccattgac atcttggtaa
    11941 actgtcataa tcggctcgct atctgcgcta taagtggttt tagcaaagca agcaccaaag
    12001 cgattgtagt ggtcaacctg acgtactcga ccttctaggt ctttccagtc tacctgttta
    12061 accaagcgag cctgcattcc atcaacataa tgaatgacag ccctctcctg cgtcacatct
    12121 tcaatacaag cagactgatt atctcctaaa atttcccaaa aatctgaaac gggaacttgg
    12181 ttaaaataga gaggttttcc atcctcgtaa cctagataat aggtaaaagg agaaagcaga
    12241 ccatcaggca gaaaaccatc tgcatcgatg accactccaa gttgagaaag accagtagcg
    12301 actagacttt catgtaaatc tcgactttca tgactataac gatcataaag ttcaatcatg
    12361 gagcacctcc tctactgttt tcttccactt ttctaaaatt tctttggtca agaagccttc
    12421 tgcaatttgg taagaatagg cacgcatagc ttccaaacga ttttcttgat acaattgaca
    12481 aatcttagcg gcataagctt gcttgatttg gtcttctaca tggtcagatg aacttggaat
    12541 caaataacca ttttgcccat cctctataaa ggtctgatta ccataaggca catcaaaacc
    12601 aattagaggt agacctgaac caatagcttc catcaaggtc agaccaaatc cttcgctggt
    12661 agaagccgtt aagtagacct catactggct ataaatctgc gaaagttccg catgcccctt
    12721 gagttggata tagtcctctg cctgatgatt tgcaataatg tctctaagca gagaatcttc
    12781 tccaccacta ccatagatat caaaggttag ttccggtaac tccttatgag cttcaatcac
    12841 agctttcaca agccaatcaa tgtgcttttc tttggcaaga cgtgaagccg taatcaatga
    12901 aaatggtttg cgcccttgac ttgaatctgt caaggaatca atactgccta caggaatggt
    12961 aacaatcttt ggctgatgct gagtatattt ggcaaattgc tcttgtagaa cttcattttg
    13021 tctatcagta gacacgataa agaagtcaac cttatctgca ttggtaaact gatagtcata
    13081 atagttattc caaaggatgt agtcctcatt tgtagcattt tcactataat gctccgcatg
    13141 aacaactacc gctaaatgtg ctgtctgtac ttcctcaaac acaacctgac caatgcctgt
    13201 ctccctatcg agaatgacca aatcagactt attcaaattc aaagatttca taaaggcacg
    13261 cacaaaagct tgctttccat agaaaatctt atccttgaaa tgataaactt cttccttccc
    13321 ttgattcatc aagatatcat agactggagt cccgtcttca ttataaaaag ttcgttggta
    13381 taagactgca acattgtcct tgggagcaaa atactcgcta caataacgcg tataagaaaa
    13441 gtaatccttc cgaatcaggt ttcccttaaa aacatactcg gcatgttgaa ccaagtcctt
    13501 gttctcatca accaaataac aggttacaaa cttatcttgg tcaaaaaaga atacacgtaa
    13561 aaccttgcca tttttttctc tgtgactttc ttcaccacca aagtaagcca agacatcatc
    13621 cactgtcacg ctagtaggtg caattttgat atctgtgaaa tgattataaa gccagataac
    13681 ctgattatca tcaaaaccaa tattggctgt taagtgctga atattatcgg ctaaaatcat
    13741 atctgtaaag ataaacttag aggacagatt taatttccga aaaacaccag cacgataggc
    13801 ttgagcgtat tcaacaccgc tactagccca accaattcct aaatttatat tgtaaattgt
    13861 catattttct cctcttatgg aaaatgagtc acgatttcac cttttggagt gacctcaacc
    13921 agccccatca ggagattacg caccatatcc gcacggattt gttctttcat agcttcaaag
    13981 cccgcgtagg cttcttgata gtactctacg attggatttt tctgactagc agattgacca
    14041 ccgatagcca tggatagctg ttgtagatag tctacctgct ctacccagtt gtcatcaatg
    14101 gctttaagca gtgaaagtcg taaaaactgt tcatataagt catgttgatt aagtaattct
    14161 ttcttttcag aaagttcttt atcaatcacc tgcttcataa agctacgaac tgcagttttg
    14221 tcagttacat ctatataatc tggaacctct ttaacatgaa aactaatatt ggtcacaata
    14281 aagtgaaaca ataattcacg actagcatag tgatcagccg ctacctcttc tgtatatctc
    14341 tcaatgatat ccacaacaac atcctctaag tcacgagaac catctattag acgatttctc
    14401 tctttataga ctatatcccg ttgaatattc atactttcag catattctag agtctgacga
    14461 cgtgccgaac ggccagcact atcactggca tgctgagcct tttcgactag tttccggtat
    14521 ttacgacctt tcaatacttc cggttgagtc atatcttgaa cctgatagtc tttgtacttt
    14581 ttatgcaccc aagatggacc aaatttcttg ataacatcat cctctaagga tacaaaaaat
    14641 ttactcatac caggatctcc ctgacgacca gaacggccac gaatttgtag gtcgatccgc
    14701 tgactttcca tccgctcagt cccaataaca atcaagcccc caagctctgc gactccttta
    14761 ccaagcttga tatccgtacc acgtcctgcc atagaggtag ccactgtcac agcccccatc
    14821 tgacctgact cggagataat ctgagcctca cgcgccgcat tattagcatt taggacatta
    14881 tgggcaatcc cttcacgaaa caagagagac gaatagagtt gagacatttc aactgagcct
    14941 acaaaaacga gtaagggatt tcccttagca tggtattgct tgatgtactc caaggatgca
    15001 tacacttttt caggtaaagt gatatataga ttatctggat agtcaatccg ttgtctcgga
    15061 cgattggttg gaatgcgtac tacagacata ttgtaagttt caataaactc tttttccgcg
    15121 accttacctg tccctgtcat accagatatc ttattaaaca tcttaaaaag actctgatag
    15181 gtgatcgagg ccatagcccg cgtctcagga gataatttga catgttcctt ggcttcaata
    15241 gcctgatgga gacctccttg aagtttagtc atttccatta gacgccctgt tcccttatca
    15301 accagtacca tctcatttcc acgaatgata tagtccttat ctttagtaaa gagcttatga
    15361 gctcgaatcg cataaaccaa atgacgagca aaagacgcat gctcttcctt gtataaatta
    15421 tcaatcccta ggaaattctc agcagacttg gcccccttag tagtgagcca aacctcctct
    15481 ttctcctctt taaagatata atcctctcct tcgaccaagg ttgttacaag tgtatcaatg
    15541 atcgcatagt aattagactg aacacgagga gaacccgcaa taatcagagg agtttgtgca
    15601 ctatcaagca agatatcatc aatttcatca ataatcacat agttaaacgg tcgtaaaaac
    15661 ttaccttctt cattcgaggc taggttatca tttagataat caaaacctaa attactattg
    15721 gttgtgtaga tgatatccga agcatagata agcttctttt cttcagcttt catctccttc
    15781 tttggatctt ccgtaaatgg tacaccaatg gtcaatccta gaaaacgata aacttgaccc
    15841 atttcctcgg catcacgctt tgataaatac tcattaggag tcacaaccat cactccttct
    15901 cctgaaaaag cgttcaaata gacaggcatg gtagctgtca aggtcttacc ttcccccgta
    15961 ttcatctcag caacatttcc atagtgcatg acaatagctc ccatgacttg aacatcataa
    16021 ggaaacatcc ctaaaatccg cttatctgct tcacgcacca cagcaaaagc ttcaaccaaa
    16081 atatcgtcta gactttctcc ctcggaaaga cgctgacgaa actctactgt tttagctact
    16141 aattcttgat ccgaaagaga ggacatcttg cctttcaggg cattaatctg ctttaaaatc
    16201 tttttcactt tcctaagctg gaaatcttga cctaaacgtc taaacacttc tgatctcctt
    16261 gatagaaaaa tgataaaaag ttaaggactc aaatccagca cttaaaagag aaacttgata
    16321 agtataacta tcttctggat aagtaaatat gaaatctttc actttttcta cctttttttc
    16381 taaaatatca ccatagcggt caaaaaaagt tatcttggta taaactccat tttcaggctg
    16441 acaatcaaaa accagtgata gttgatactc ctgctttctt tttaataagg gaagctgagg
    16501 ttgggttcgt gcagcctggt aattgacact agaaaaccaa gtctttagaa tctcaccaga
    16561 tggcaataga ggattataca gacgaacact tttgtcaggg taataagata cagttgttcc
    16621 atacatataa gtcccaccaa cttctcccca atgaatagac tgtctctgtg taataatcat
    16681 cttttctccc ttccaaaacc agtctctaag accattctat aaaagtgcaa aaaccaagaa
    16741 atattggtat ctgtatcatc attatgtcgt ccagcagttc ctttagataa aatcttcgca
    16801 cctgtattac acagataaga cactaattgt tcataggctt gtggatccat ttcttcatct
    16861 ttcatatagg ataaaccaaa ggttgtcttt gaaaaatttg cttgtttaaa aattttccaa
    16921 aaacgctgat ccaactccgt catatcttga gaacttgtcc cacctgtatg atgaattaaa
    16981 caatcaaaag ctaagttaga gacacccggt gcgtccaaac gtccacgact agctatagtt
    17041 cctaaattag ccaagggctt accaactatg atggcatggg gttcaaaaga agcaccataa
    17101 tagagagccg gaaacgttcc catagaaaga cctgataaaa tcaaatcctt atgatcaaag
    17161 cctaaataat caagatagtg agtgatcgta tcctttactt ttccctctag ctcatccgtt
    17221 ccgagataaa aagctccccc ctctaaacgt ggatcagaaa ataaaatgaa gggacatcca
    17281 agagtcttca tcataaagta accctcgaat ccttctgcag gtcgataacc tgcaaaatag
    17341 acagtcaaag gcggtttaaa atcacctgga tggaagaaat agtttatttc atcacgcttg
    17401 gaatcatgta ggatatttcc accaagtaca aacttaccaa attgttttcg actccatcgt
    17461 tggtgaagat tacctagttt gatagttcca cgacctcttg cttcaacaga tatcgagaga
    17521 aagtagtctc cgtctcgact atccataaaa agtgcttgct ccaagtcttt ttcagatact
    17581 attacttctt caaagaaatc atcaacagac ccactccaca ttttacggat tactaaacga
    17641 aagtcacaat tgccttcctt ctcatattca agccacaact caatagggag tgttttttgc
    17701 accataatat tataagccca ataggcaagc tgggcaaaat cttgcccaaa ctcaccttcc
    17761 aaactgacat aatccaatcc ttgataagaa atagctcctg taaaatttgg attgacttgt
    17821 atcgtcggag gaaagagttt atcaccatac ccaccgccaa aaagagattt acttaaatca
    17881 ttgattagtt gttggggact taaaaaatca attgcttgtg cacatcgttt ttttagaaaa
    17941 tccacaacct ctctatcatt tatttgtaga ttctggttat aaaataaagt ataaggttca
    18001 aaatatttta cataggaaaa taagtctctg gaatactgac catcttctat taaaactgca
    18061 tgaaaacggt taatatcctc tttttcaatt atttcacgaa gggcttttga tgagtttggg
    18121 taaaaataat accaactcat attttcaggt atgtcatagt gattttccca attggcaagt
    18181 cctatctgta aaatattaag ttcctttgac atttttaacc tcctctatcc atttctctaa
    18241 ttttccaaga aattgttgtc ctgtgtgctc cttaatctta tcaattgaat atatcaagga
    18301 ctcattccac tccttcaacc tatctgtgta ataatgtgca gccttagaaa attctgtaac
    18361 atctgctaac aaataaccat tttttaaatg ttcaacatag acggtttcaa ctaggttgat
    18421 ttgaggaatt cctgcactaa tcccagcaat ctgtgtgtag agatgaggat gtctatttaa
    18481 atccacaatc aaacggacaa attctagtgt ttttatcaaa tctaactcat cattcaagtt
    18541 aacaaactgt aagcgtaagt cctggtgttg attttcttcc agaggatttt ctgcatcacc
    18601 ataatcaatc gcctttccca gattttcgga ttgaatgttt tcttggatga aagactcaac
    18661 aatcccttca acctcattca tttgctcctg actagcagca aaggctccaa aaatcacctc
    18721 agtatcctta ttttcagcga caaaggacaa gacttgaagc agagcttgat tatcaatccc
    18781 ctgctcaaaa tccagttgaa aataaatgat ggattctttc ttagtttggc ttcgtcccaa
    18841 tcgtaatcgg gtgtcaaaag atgataaatg ataacattga tgcattcgtt caggatacaa
    18901 ctcctgcaac aatcgtaaac tatcctctct atccaccaaa atcaaatccg atttttcaaa
    18961 agttacatct aaatccctaa aggtatcttg aggattacgt ccaatgaaca gactcaacag
    19021 tttaggatta gtactaggta aacgatctag taccaactgg tcatgatgag aatgagaagg
    19081 aagcataaaa atatcctgat ccttcacata cgtttgcaga taattctcaa aaaattctgc
    19141 caccagatct cccatatttt gataggtaag cattttaaag cgataaccca aaattggatt
    19201 gacttctacc tgtcctcctt cttttaaacg ctctctaaat tgccaaactc ccttaggatt
    19261 caaatattct tgataggctg cttgcccatc ttcaaaaaaa ataatactcg atataaaccc
    19321 tcgatcatcc atgatatggt gcatatgtat ttgaccattc ggtccaaaat actgtatatc
    19381 actgatgaaa ccttcaactc catgttcaac cttagcaaat ttcttcccat ttttttgaac
    19441 gataatcgta aagggactat aaataaattc acagtcatca tcccactcaa tatctctaat
    19501 gcttaaaact tgggtatgga gattatgaaa atcttgcata acatcaaaaa cggaataagt
    19561 atccgtttct aacacaccat gtctatataa aaaataacgt agatgaggct ggtaagctaa
    19621 tactaataga cgagaatcta tatcttgctc ttgaaagagc cgaatctggt gaaaggtatc
    19681 atcaaactct agacgaaaat gagaaaaata ccatggagtg atatctgcat gccatgttct
    19741 ttctgaccca taccaagctg gaataaaata atacataaaa gtctcctaaa tcaaaggtct
    19801 ataggtctca tttagcctta aagccttgac ctcgtctcta atcgtaaaaa tcatgccccc
    19861 gaacatcata aataagcctg gaatcattgc caatcgtaat aacttttcat caaacaaaac
    19921 aaaaagcatg ggaccacctg ccataatcac attaaagaga ccacctatga ctgagaaacg
    19981 aaggaccaat cgattaataa atcgactagt atccgcacct ggataaatac cataaatgta
    20041 ttctccagat tttttcatac ggtctgcaat ctcttctcca ttcatcgtaa caaaagcaaa
    20101 aatgatacta aataaaaata agaccgaaat ataaacatag acccacaaag gcttcccaac
    20161 cataaattcc tttgataaag ccgctagccc tgaatgatta gggaaaataa atcccaacaa
    20221 gatgaacaaa taagctggta cactaagaaa actcatcaca tacatataag gcatcccacc
    20281 tgcaggattc aacatgattt cgagataaga atagcgttta aatcgattgt gtaagccaat
    20341 tttattaaca ggaaccaaat agcgagctcg atacataagg gcaagcaagt aagaaaatat
    20401 aatggtcata aaaacaagca agacaacaat ccctgtaggg atataaacca gtttaaaagt
    20461 ttcaataaca tcctgaggga tatttaaaac aatgctggat aggaggatta caatagaacc
    20521 cccaattccc atgctagcat ttaaatctga caaccaaaca agaaaaaacg ttcctgctat
    20581 gagtaacaag gtattcatta gagcaaccaa aattaccgag taggaggatt gtactggcaa
    20641 gctcaagcta actgccaagg attgaatcac agcaattagc aaggtcagat acattcttcg
    20701 acgatcttga atttctatag acgtagatgt taaacccaac cgtttagaaa aagaaaacat
    20761 ctgccataaa atcatggcgg acatccaagg ggataatcca acagaaaaaa ttgataaact
    20821 tcttagattc ccacctgtta gggcggctga gaaggctaga taggctgttg aacctcctaa
    20881 aaaatcttta gtatttaggt caacaaaagg gagagtaata cgacttccca atacatagat
    20941 aaacagtaga aataaggtaa atagtccttt ttttactgct attgacgaat aaatcttagt
    21001 cataaaacac tcccctcacc ttcattgttt gtgatatggt aaataacaat tcttttattt
    21061 caacttttac catttctttg ttctgcatca aaaaacaact ttaatctgaa acaaatatct
    21121 ttatattcct cagtatctgt taattgatgg tattctaaag tttgcttata atcttttaaa
    21181 agattgacaa atcgaatacg cagaacggag acatccaaac cagccaagac acaatccgat
    21241 attttcttag aaaacacttc aacaatatct cttgcccagg aaagatcaaa ttccgttctc
    21301 atgatgcttc ctgaacgtct tctatacaga tagagattat cgtttataaa gacgatttta
    21361 gaggctaaaa gataaaagcg atgcatagtt gcttcatcat caaagcggcg accatttgaa
    21421 aagtgaactt cattgaataa ttctttttta atcaacttaa atgtcggcaa tataaaggcc
    21481 gagctattga atttccaatc tcctgcttga cggttcataa tttcttgtat agctaattct
    21541 tcgacgcaaa aatctttccc tgttacataa aaataaaaat tcccgtcact ttcgtcataa
    21601 ttatagtaat tccctataac aacatctgca tccttttctt ggacttgctg ataaagagtt
    21661 tcaatcatat tttcttttac gatatcatct gaatcaacaa tcgtgatgta ctctccctta
    21721 gctacactaa tccccatatt tttagcttgt gctgcccccc ccccttattt tcttgatgca
    21781 atactcgcac acgattatct tgcatcataa aagcatcaca aatttcccca gaattatccg
    21841 tagaaccgtc atcaactaaa ataatttcta tattttgata ggtttgcttc agaatatgct
    21901 cgacacactc cactaaatat tttcccgtat tgtatatcgg taccacaaca ctaattaatt
    21961 cactcattac tctatctcta tattcttcat tctatccact aaatcatctg gtctctccac
    22021 agaaaaaata ccgtcataga gtccatcatc catactttta cgtgtgatat caaaagcgaa
    22081 gattttcttg ccactttctt gtgcccttgt aactacatta aaaacctctc cacctgtatt
    22141 aatatctaaa taaactatag aatcgtccaa tagccaatca attctactat gtaatacatt
    22201 ttgatatact gttacattcg tatactgtga tagagaggtc agctcctcag aacaatcaca
    22261 cggtgcagcc aaatgaaaat gccaatcagg taaccgttgt actaaatact ctaaatgttt
    22321 tatatctgca ctccatgtca caagcatcac ttgtttttta gactgattgc ttctttcaaa
    22381 ataatttaaa tcggaacgtt gaaaagcaat ctctgaccaa tccaaatctc tataaaccca
    22441 ccataattca cgtagtctag atatactata tgtattccaa ggtttatcat gactagcata
    22501 gtgaacaatt gtaggtggat tgtcatctag acgttcacat tcttgagcaa ggtgataaat
    22561 atcaacacct accatataat tatatgtttt atctaaggct agccaattat cctcaaaata
    22621 aatatttaaa atactctgat ccccaagatg aacaacttga ttttgctcgg ccgctaattc
    22681 caataaacta ttgacaatag agtgttcttt ccattttgca acatccatta ataaaactcc
    22741 agaattaaat ccagattttc gtccttcata ggcatagaca tcatcaacag caccaattga
    22801 atatcctttg agatctatct caaacaaagt agctagttcc ccagttacaa tgatatcgga
    22861 atccagatac aataccctat cagattcaac cacttctgtc gcaaaaaatc taaagtaaga
    22921 agcataattt atatgaggtc ctgttttata gctttcaaaa agttctttat caatgtgtac
    22981 actgcgaatt gtagaattca caacttccat ttttctcccc aataatttaa accattcagg
    23041 agctatatca ctgttgagaa tataaaaatc aacatctcta ttgtgataca atacagactt
    23101 tatagtcgtc tctaaaggaa taagatagac attatctgcc gctaatacta ttgattttct
    23161 catactgttc tctacctcat gtaagatctg attgctgcaa ccattttttt cggaatggag
    23221 cttggataga taccttcata tgaaatctca gaaagatttt cagagcaagt attgtcgaaa
    23281 gctatcattg gttttttgta cttaagcaca aactcatatg catcttctaa ttttcgatca
    23341 tgattcaaat ccaaatacaa atctgaagct attactattt gttccaataa cattggtaag
    23401 gaatttggat atacggtaac attaggaaaa cgcgttaaag ctagtagttc atttgccata
    23461 tttgtataag caacaatatg aaaatgaact tctggcaatt gctccgccaa ataatcgatt
    23521 ttctcgacac accaagaatt cgttaaatta acacattgca acttaaatat ttgtgattta
    23581 ctaggatact tcacactctt tgaaaaccat tcatttaaaa taacagacca atccatcaaa
    23641 gagtattccc accaaacttc tcttagacgt ccaacagaaa attgattcca aggcttatcc
    23701 tgagaaatat agtgtaaaat tagtggcagt ggttcgagcg gaatatcaaa aatgaattga
    23761 tgtttaaagg ctgccgcccc ataatcatat cctatttgaa aattatattg atcttcaagg
    23821 gaactatatt gatctttaaa caacatattc aaaattgact ggtctccttc ttccacattc
    23881 tcatgttctt tttctgttaa gtcaatcaat ttttgtcgaa tagtttcaga tccccatttt
    23941 ttgttgttaa tcaagagaac accagcattg aagccgactc ctgctccaaa gcaagaacga
    24001 gctgctgcca aataattttc acctaagtct aattcaaaca aatcggtcaa atcaccagtc
    24061 acaatcaaat cactatctag atagagaact ttatcttctg ttacaaaatc tggaataaaa
    24121 tagcgtgcaa atgtcatatg attgatatga ggtaatttat tagaccaatt catttgaaac
    24181 tgtgagccaa ttaacttgca gtcaatcaag tcgcccccca tctcttgtaa atatattctt
    24241 atttgactaa accattcctg aggaatgtcc tgatttagca gataaatttt caaatgacta
    24301 ttgtgtctac agagtgactt catcgccgtt tcgatttgtc gaatataagc gtaatcacct
    24361 gcaaatacta cagatttctt atcttgtaac attttatcgt ctctctatct taattttcag
    24421 ttatatttat taccattcaa taaatagtct aacatttcta aatgtcttgt ttccacattc
    24481 tccttaagtt ctcgttgaat tgcaatatca tgacgatatt tcaaatagaa cttcatctgg
    24541 tctaaatatt tactgacatc atatcctttt agcgctagca cggcaattcg ttctaaccta
    24601 aaatcacaat catcacgcat catcttcggt gaataagaat tagaaagtcc ttctttccca
    24661 accctatacc aatacaaaac tttattaata taggttactt tctcaacctg cataaataat
    24721 ttataattgg ttcctatatc ctcacacacc cgaccaaacg gaaactgaac ttcttgaaat
    24781 aattcccttt taaatagaat tccccaagaa gttaaaaagc tcatatcata cctctctaat
    24841 aaaggaagtt cttccattag aagttttgaa ttataatttt tcacataata atcggagtca
    24901 aatacgtgaa tatagaataa gccatctgac tcattatagg ttgaataagt tgaaacaaca
    24961 atttcagaat gctgctcctt caaagtttct ataagaattg ataaatactc ttcagtaacc
    25021 caatcatctg agtctacaaa agtaatataa tctcctttag atctctcgat tccaaaatta
    25081 cgtgcatccg ctaccccagc attttctttt tcgaaatagt gaaaacgact atcacgatca
    25141 gcatactctc gacatattgc tcctgaatta tctgtagagc catcatttac taataaaact
    25201 tcaaaatttt gataaatctg tttcgataaa ctgtctaagc agctagaaat aaatggttct
    25261 acgttataaa ctggtactat aacacttatt aaatctttca tacttctctc tttgataaat
    25321 caactaaaat ctgactgtta taggtttcat cctcaatgaa ctgaatatct tgatcaaaat
    25381 tatactctgc tgtatgttta tgtgaaaatt taaccttagt tttagcaccc atcttcatcc
    25441 actcatactc ggagtcaata tgcccaatat ctaagacttg gtaacccttt cgacttaaat
    25501 gataggctaa aacttttgct gtaggaccca gcatacataa aatcagtctt ccttcagcat
    25561 gtttcataat ttcctcttga atgttatcga caacggaata agcattatga gatggacaaa
    25621 taatccgttt cacagatttg accttatcaa ataaatcatt tccaacccca gagcgagatg
    25681 taatcccctc tacaatcaga atatccctat tctcccaaat actctttagc ttttcaaatt
    25741 gtccttttgc cttactctta tcttcataat caatgtatgg acgcgagata aaggtagagc
    25801 cataccatga acccttacac aactctttat aaaaatccat ataaacattc atatgttctt
    25861 tccagaatgg tgtagcccac caagtaaaga caaaacgatc gtcaaaagca tcaggtaaac
    25921 aaaccactaa tttctcgtca ctctcaagac ccacaatatg ttttaattgg ttagctaact
    25981 ctagatcatg attttgataa ggtataggca aaccccacat caaattgact tctccgtctc
    26041 caaaacgaac taaagaagag ttgtgttcaa taatataatc caaggtttca tctatccctt
    26101 tcacctggat tatatcaagc ggtttttcta gcatatcctt agttatcaat ttttgcaaat
    26161 cagccaacat tcgttcagga tgttcacttg aatacaacct ttggccatta ttcccatttt
    26221 gagatttata aaaggctagt ataggtttag atagatgagc catttctgca acgatatcgg
    26281 atgttgaagt cagtaggttg atatctaaat aagcatccat cttctccttc aactcgtcta
    26341 gcttatctct agaaattgcc ggatagagct gaatattagg ataacgatta gacaaatcat
    26401 ataattttgg ccccatagct gtccaagcag caacttgaaa aacaacattt ggcaactgtc
    26461 cagccaagta atccaattgt tcgatatcct gagaagccgt aaacgttaaa caagttaaac
    26521 ttggaagttg tcgaaaacag gtaagatgtc cgtttttctt aaaatcttta atcgtatcaa
    26581 tcataaagga aactttccca ttttcgaaaa cgatttgtcc taactctccg tgctgagtcg
    26641 attgaaaagc tagtagcgtt ttttcttgtt cttgcaaaga ttttaaaaag ttctcatctg
    26701 cactaccatg attgatatcc aaatatagat ttgttgactt tttcagctta tctaatacag
    26761 gaggaacaat ttgtggatgc aatctcacat tattatatac agctaatttt tttaatttat
    26821 ctcccatatc cgtccaagct gcgatatgaa ataccacctc aggtagagct gtaactagct
    26881 cttctattcc ttctaaatct tgggaattcg ttaaggttaa gcaagaaaat tccttatcta
    26941 gaggcgatat tagttcaaat tctcccaaat gatgttgtaa aatctgattc cactccaaat
    27001 catggaattc ccaccataaa tcacgataac gattggctgt caaggtggtc cagggttttc
    27061 tgtaggtcgt aaaatgaatc accactggtt tatcattaaa agccagatgt tcctgccaat
    27121 tgttatacaa agccacaata tcatgcccta cttgtaaatt ataagctcga cctagttcta
    27181 accaatcatc ttgtaagacc tgattaaaaa tcgtttgatc accattaaaa tgctcgaaac
    27241 ggccttcttc aacttccttc attgtaacaa tgctctgttc aattagtcgt tcttttaatt
    27301 tctcttgacg ccatttttca ttgttgatta gtagaacacc agtattaaaa ccttgaccat
    27361 ttgcatctct aatcgctgcg atataatgtt ctcttacatc ctgctcaaat agactatcta
    27421 atttatcatt gataagcaag tcacaatcta aatacaaaac cgtatcttct tgtatataat
    27481 ctgggataaa atacctagca taggcaattg aactaatgtg agcctgtttt ttccaatctt
    27541 gaaacacagt ttgttcaggt agtttaacat cgataatctc actacctaac atgctagcta
    27601 tttttcgtgg ctttcgaaac caatctggca tgatatcttg atttaaaata taaatcttaa
    27661 catctcgatt gtggtataaa atagatttta tggttgtttc taactgcctg gtgtaattac
    27721 gattaccagc taatacaatt gttttaatca tcttgttctc ttattcaact cccaaatggt
    27781 aaattgcatc taccaataat tttttagtaa aataaccttc ttttagcaaa taactaaagg
    27841 tttttatccg attcgtcatt tcttgatatt cttgtagatt cattttatca actatctcat
    27901 gaacttcctc tagactattc gccacaaagc ccaagccttg atctactata aatttagcag
    27961 ttgataagtt gctcggtaca atcactggga taccagctgt tagataggta ctaaccttat
    28021 gagatatatt caaggtatag tattggttac tttctccctc attttgatgg gttccccaga
    28081 caaggccaaa tccacccttt gataattcta gcaacaattc ctcatctttt ttccatcctt
    28141 cgatgctgag atttctagca ctagaaccgg cctccccttt atttgaaaat actctcaaag
    28201 gcgtatcttg agaccaattt tgtaagtctg gaaaacgctc taaacttcca gcaaaaaaag
    28261 ttcttttttt aaaagcaggg gtgtataagg ataaatcatg aggatgatcc cacatccctt
    28321 gaacaagaat cttcttagtc gtcaatcctt cttccatcag gcgtgttttc attctctctg
    28381 acggcactat caaaacatct gatagattat acatgtacat atagtctttc atgagataat
    28441 agttactatc aaacatgagg ggaacaacat catggataaa gcaaataatt ttcacctgca
    28501 tatcctttag cttatcaaac aagagacgat caaattcaaa gccattccag gttggagact
    28561 gaaagactaa gatatcacca atagagatac tggccataat accatccaga cgcttattca
    28621 tttcagaagg actatctgaa gcaatgttgt aaaaataaat accaacctct ctaaatccca
    28681 gttgactagc tatcttttga acagcatttt gagctaagat aaccgtacta tcaccagcca
    28741 tgccgtataa atttgttaaa tgtaatttca tattacgcta tctcattttt tattactatc
    28801 tacggaagta aagacaattt ttccatcatt cttctataaa tctctgtgaa ctgcatattt
    28861 tgttcttcta attttgctac tactctttgt aatctatttt tataaatcaa aatatgattg
    28921 gtcaagtcat agtctgaact tgctaaaata gctatctttt cttccctagc cccaatttca
    28981 tcatacatac gcttttcact ccatgtattc gataaagtat cagataaacc aacacggtac
    29041 cagtaaacac aacgattcaa atagactatc ctcgaagctc ttagaagtac cttccatgtc
    29101 cagtaagtat cctctgcaat ttttcctata ggaaatggta aatcttgtag taactctctc
    29161 ttgaataact tcaagacagc tacagtccag ttcccatttc tgacttcttc gacaccttcc
    29221 ctatccataa ttgctttacc ttctatcact tctagagaat catctggatc cgtaacataa
    29281 gtcatataca catagcgtgt ttcatcataa gaattataac gcccgatact aatatctgcg
    29341 ttttcctttt tcaaagcacc atataatcgg tctaaagcat catgttccaa ccaatcatca
    29401 gagtctacaa aagtaatgta cgcccccccc gaacattcaa tacctaggtt acgagctgat
    29461 gaaagaccgc cgtttgcttt ctcaaaatat ttgaaacgag aatctttctc tacaaattct
    29521 tcacatattt tggatgaatg atctggagag ccatcattga ttaataaaca ctcaaaattt
    29581 tgatacgtct gattctgaat gctatccaaa cacattctca gataattttc cacattatat
    29641 attggaacaa caatactgat taactcattc tccattgact tattctccta aaaatttgcg
    29701 aatagcatct accatcttat ctggctcagt tgctgaaaaa atccgacttc ttcctttgct
    29761 atcatgacta gtattatcaa aactaaagat tggcttattg cgcctacaga cttcttctat
    29821 aatgttgaca atctctcctt catggttaat atcaagatag aaatccatct tatccaaggt
    29881 ttctttcata ttaaaagaat taaaatttgg aaaaagtgaa agattcagat atctctgtaa
    29941 atcaacaaca gagggagcaa aatttgtatg cgccaagatc gtaaagtgca cttctggcaa
    30001 ttccgatagg agatattcca aggactccat tgcggcacta tttgtaaaaa tacaagtatg
    30061 atataaggga acacccacta actccttaaa agagcgttta acgatatctg aacttaacaa
    30121 cacatcgttc cagttcaatc cataataaaa ccaccagagt ttacgaaaac gtgtaagtgt
    30181 taaatgagtc cacggtttga actctgtagt ataatggaca atcttaggct cgtaatttcc
    30241 ataatagttg gattggtacc agtcagcatt accttttaaa tgtaaaaact gatctaaacc
    30301 aaccatgaaa ttatactctt tgtccaaatg tagccactgg tcaccgaaat atatattgag
    30361 aacaccttgg tccccataga catgttcatg gtgttgagca gtcaactcta gcaaattttg
    30421 tgttgatttt ttttctcgcc atttttttgc atcaatcaat aacatacctg aattaaatgt
    30481 tgttaaccaa ccatcttgat caaaatcttg aacagctgcg atagcgtatc cttgcaaatc
    30541 caaaaaaaat aattcatcca aattggcatt cacaatgata tccgagtcta agtagagtac
    30601 ccgatcttca agcacttcgt ctgctatgaa atacctaaag aaagttgcat aggacaggat
    30661 tgcatttggc aagtggaaat cacgaagatt gtgatctgct tttttgacat taataatctc
    30721 agaaccaata accttcagac gatttctcat caaaagaaac cattcactag gcagatcatc
    30781 attaaacaca taaaatttca cttcgtgatt atgaactgaa actgacttta taacggtttc
    30841 taccttatca agataatgaa tatcagctcc taaagcaata acttttctat acattcttat
    30901 ctcccgattt ttctatttta taaacaaatc atctatttta ccaactgaaa aaacgtgatt
    30961 tctacttttg agatattgat tttttacttc tgtttcatca acaattacag tatccttttc
    31021 ttcgatgata tcttttcatt tctaaatcta aatgttgaat caaaaaatct tcaaatcaat
    31081 tatgctatga ataagatttc tttcgtgttc tgcaaaattt acaactatct acttatagat
    31141 tcggcattat caaatagatt aaagactcac cattaagata tcctctgaag tcatgtttgg
    31201 ttttcttcca accttagaaa gtttcatcta acttgatttt atattactca ttaaactctc
    31261 atctttacaa aaagatttcc attattataa actacactgt ttgatggaat atcattgcgt
    31321 atgagacaac ctgctccaat aatggtatta tctccaatcg tcactccttt taaaatcgtg
    31381 acattacttc ctatccagca gtctctgcca actctaatcg gcgctgtagt ccactgccac
    31441 ttttcaatct tttcagcggt ataaatatgg tcgtgatcat aaaaacgaac accctctccc
    31501 atcatggtac cagctccgat ttcaattctt tccatacagt taaaactaca agagttgttg
    31561 ataaaaacac cattattcag gattaactta ccagaagaga catgaaaatt ttcaaaattt
    31621 ctacaggcga cattggctcc tacaatgaag tgagccttat catcgatttt aaagacattc
    31681 caagtcccat cttgaatgat tttctcttga tgattggtgc ctactctgac ttcattcaga
    31741 tagtctctca ctctagaaac caatttgtct ggtgtacttg atagaaaaac ttcctgcccc
    31801 tgatttccat ggactgtatt gtcaaatgca aagacagatt ttccagcctg tacaaacttg
    31861 gagacaatag catctacttc ttgaaagtga ttgatatcta gtaaaaagtc acaggtatcg
    31921 actaaaaagt ttagctgttc ttcatttgta atcgttcgga gacggacatt atagttcgtt
    31981 tctaagctac ggattttctc ggaaaattga acaggtgctg caatatgaaa acagatattt
    32041 gacaaggatt caaggagata atccaactct tcaatctgat cactaaccgt caaaatcaga
    32101 gcatctccta ctaaatgatt tttcttcatt tgcttatttc tctcaatttc tcaatcattg
    32161 cttgaacttg gtcaacagca tatgcctcct gacctacttc ataggtttta gtattttcaa
    32221 aggataagat aggcttgcca agattagcaa attgatcgag aatttcttct gtcttttcgc
    32281 catgattaat atctaaaagt acttgactgg tttctaccaa ttcattatcg acatctacca
    32341 aatagtgaat tccgttaaat atagtcacgt ttggataaat tgtcatctga gccaatcgat
    32401 cactaactat tactctagct gctatcttaa actgaatatc aggcaaggat tgaaccaatg
    32461 tctcaatttg ttcaatatgg tctgaggcag tatagattag acaagtgaaa ggttccttta
    32521 ttggatagat gtgagatctt tgtaatggat gtaaatgatg gttttgtccc aattctgtcc
    32581 attcaagccc atgatagtac caccaaactt cacgataagt ttgggccgcc aaatctttcc
    32641 acggttttct atgggaaagg tagtgaataa tagcaggata atcctgaccc tcaggcaatt
    32701 gataatcagc aaactgttta tgaatgacaa tatgattata atcaaaatcc aattccagcc
    32761 atttatgttc aaaaagcata ttcaagatgc tctgatctgc ctgatccacc ttatcatgcc
    32821 attcattggt taaatcaatt aatttttggg tcatattctc ttttttccaa aaagcattgt
    32881 ttaccaagag aacaccggca ttaaagattt cttgaccaaa ataagctctg cccccaaaat
    32941 ctctaacagc agccaaagga taatcttgta agtctgtagc aaacaagtca tccagatttt
    33001 tcgttacaac tagatcacag tccaagtaga gggccttgtc ttcttgcacg aaatcagcta
    33061 tgaaatagcg taaaaagact gtgtaactaa tatccgattt ataacatgaa atttgctcag
    33121 aagttacccg acaattaata atttctgagt caaacttctc taatcgctta tttaattgtt
    33181 tgatccattc atttggaaaa tcgctatgaa tcaaataaaa acgaagcgag cgattgtggt
    33241 aacagataga cctgattgtc gttaaaactt ggtctacata ggcataattc gccgctagga
    33301 caatggcttt cttttcactt gattcttgta ccgttaactg atttaaaagt ctttttttca
    33361 tttcaaactc tttatacgtt gctgtgtcag ataaaccact agcttgaccg ttggcgagac
    33421 tgaattccaa catttgacga taaatcgcca aatgcttttc taaagggtaa cccatattag
    33481 ctagtagcgt aatgcgttcg gacatgacat caaccaatgc atgcatccac ttttctgtcc
    33541 aaactcttga taaactacct tttctaatcc gattattctt catcatcacg tttacggcgt
    33601 ttcgcaaaca aacccaagcc agtcacagct gccaaagctc caaggagtac agatgacttg
    33661 gaagcttctg tacctgtatt tggcaattgc tgtcttgact tacctgctga agttgacgcg
    33721 cttgcgcttg ttgatgccga ttcagacgct gaagccgacg cactcgttga tgctgaagct
    33781 gaagcgcttg tcgatgctga ctcagacgca ctcgttgacg ctgaagccga cgcactcgtt
    33841 gacgctgaag ctgacgcact ggttgacgct gaggctgagg cagacgttga tgcggattca
    33901 gacgcacttg tacttgctga agccgaagcg cttgtcgatg ctgaggctga agcactggtt
    33961 gaggccgatt cagaggcact tgtacttgct gaagccgacg cactcgttga cgctgaagct
    34021 gaggcgcttg tgcttgctga agctgaagca ctcgttgacg ctgaagctga cgcactcgtt
    34081 gatgctgaag ctgaggcact tgtcgatgct gaggctgagg cactcgttga cgctgaagct
    34141 gaggcgctgg tacttgctga agctgatgca ctggttgacg ctgaagctga agcactcgtt
    34201 gatgctgaga ctgatgcact ggttgacgct gaagctgatg cactggttga ggccgattca
    34261 gaggcactcg ttgacgctga agctgaggcg ctggtacttg ctgaagctga cgcacttgtt
    34321 gatgctgaag ccgacgcact cgttgacgct gaagctgagg cgcttgtgct tgctgaagct
    34381 gaagcactcg ttgatgctga ggctgatgca cttgtgcttg ctgatgtact tgcgctggtt
    34441 gacgctgaag ctgaggcact cgttgacgct gaagctgagg cactcgttga tgctgaagct
    34501 gaggcgctgg ttgacgctga agctgaggcg cttgtcgatg ctgatgctga tgcgcttgtt
    34561 gatgcagatt cagaggcgct tgtcaatgct gaagctgagg cactcgttga tgctgaagct
    34621 gaggcactcg ttgatgctga agctgaggcg cttgtcgacg ctgaagc
//
LOCUS       DAVKLX010000017        34475 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKLX010000017 DAVKLX010000000
VERSION     DAVKLX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34475
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.017"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            573..1025
                     /locus_tag="VW325_000909"
     CDS             573..1025
                     /locus_tag="VW325_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9734338.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGEVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1044..2381
                     /locus_tag="VW325_000910"
     CDS             1044..2381
                     /locus_tag="VW325_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9734339.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2460..3344
                     /locus_tag="VW325_000911"
     CDS             2460..3344
                     /locus_tag="VW325_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9734340.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3344..4177
                     /locus_tag="VW325_000912"
     CDS             3344..4177
                     /locus_tag="VW325_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9734341.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4196..6289
                     /gene="nanB"
                     /locus_tag="VW325_000913"
     CDS             4196..6289
                     /gene="nanB"
                     /locus_tag="VW325_000913"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU9734342.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6302..7405
                     /locus_tag="VW325_000914"
     CDS             6302..7405
                     /locus_tag="VW325_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9734343.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7579..8277
                     /locus_tag="VW325_000915"
     CDS             7579..8277
                     /locus_tag="VW325_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9734344.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8296..9813
                     /locus_tag="VW325_000916"
     CDS             8296..9813
                     /locus_tag="VW325_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9734345.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9938..11266
                     /locus_tag="VW325_000917"
     CDS             9938..11266
                     /locus_tag="VW325_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9734346.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11518..12384
                     /locus_tag="VW325_000918"
     CDS             11518..12384
                     /locus_tag="VW325_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9734347.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12400..13239
                     /locus_tag="VW325_000919"
     CDS             12400..13239
                     /locus_tag="VW325_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9734348.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13511..13963
                     /locus_tag="VW325_000920"
     CDS             13511..13963
                     /locus_tag="VW325_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9734349.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14105..14413
                     /locus_tag="VW325_000921"
     CDS             14105..14413
                     /locus_tag="VW325_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734350.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGTYAAKYGISMARSI
                     LSRAAATAAARVGLSTKISGWILRVAVNVADVYGNFANNIAAAWDAHDKIPNNGRINF
                     "
     gene            14423..14638
                     /locus_tag="VW325_000922"
     CDS             14423..14638
                     /locus_tag="VW325_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734351.1"
                     /translation="MKALCIFLLNMLAWTVLAMIIRGGLDGFDEHTWSTILIASLFGV
                     YDYKPIDKNRKSPKEKRKNRFVHGRDL"
     gene            14696..15340
                     /locus_tag="VW325_000923"
     CDS             14696..15340
                     /locus_tag="VW325_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9734352.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSAFLLFGFGLLAFLQVGVMEKLFAKYE"
     gene            15513..16430
                     /locus_tag="VW325_000924"
     CDS             15513..16430
                     /locus_tag="VW325_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9734353.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEILKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVIEEDRPVVEAAAALIRETKERFL"
     gene            16448..17332
                     /locus_tag="VW325_000925"
     CDS             16448..17332
                     /locus_tag="VW325_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9734354.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17348..18199)
                     /locus_tag="VW325_000926"
     CDS             complement(17348..18199)
                     /locus_tag="VW325_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9734355.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18399..20456
                     /gene="pbp2b"
                     /locus_tag="VW325_000927"
     CDS             18399..20456
                     /gene="pbp2b"
                     /locus_tag="VW325_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9734356.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKELP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20467..21063
                     /gene="recR"
                     /locus_tag="VW325_000928"
     CDS             20467..21063
                     /gene="recR"
                     /locus_tag="VW325_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9734357.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21239..22282
                     /locus_tag="VW325_000929"
     CDS             21239..22282
                     /locus_tag="VW325_000929"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9734358.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPGLIERLVDL
                     AKESFDKREAHLI"
     gene            22366..23739
                     /locus_tag="VW325_000930"
     CDS             22366..23739
                     /locus_tag="VW325_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9734359.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDTVIFYGQDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23726..24337
                     /locus_tag="VW325_000931"
     CDS             23726..24337
                     /locus_tag="VW325_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9734360.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYTKFVFGCKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24428..25132
                     /locus_tag="VW325_000932"
     CDS             24428..25132
                     /locus_tag="VW325_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9734361.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25351..26724
                     /gene="ftsA"
                     /locus_tag="VW325_000933"
     CDS             25351..26724
                     /gene="ftsA"
                     /locus_tag="VW325_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9734362.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26741..28000
                     /gene="ftsZ"
                     /locus_tag="VW325_000934"
     CDS             26741..28000
                     /gene="ftsZ"
                     /locus_tag="VW325_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9734363.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            28005..28676
                     /locus_tag="VW325_000935"
     CDS             28005..28676
                     /locus_tag="VW325_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9734364.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28686..29225
                     /locus_tag="VW325_000936"
     CDS             28686..29225
                     /locus_tag="VW325_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9734365.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29225..29488
                     /locus_tag="VW325_000937"
     CDS             29225..29488
                     /locus_tag="VW325_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9734366.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29485..30270
                     /locus_tag="VW325_000938"
     CDS             29485..30270
                     /locus_tag="VW325_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9734367.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEYL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30279..31067
                     /gene="divIVA"
                     /locus_tag="VW325_000939"
     CDS             30279..31067
                     /gene="divIVA"
                     /locus_tag="VW325_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9734368.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31415..34207
                     /locus_tag="VW325_000940"
     CDS             31415..34207
                     /locus_tag="VW325_000940"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9734369.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPVGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     ICFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEAQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10598 a         6081 c         7504 g        10292 t
ORIGIN      
        1 aattgaaaac ggtaacaaaa ctaatttaga gaatgaaata tagagtattt ctctcttaaa
       61 agtttttggt gaaacgagat gtagaaagga gatttagcca aagagtctat tagtgctaga
      121 ataatagatt agaattattt tagaaaaacg aagtgagcag cttataaatt caagtcccca
      181 aatagattca tactagtatc ttttgcaaaa aataaagggc gatttccttc atgaatatca
      241 atttcatcta taaggaaggt agctaattga actaacttat ttattctgtt tgtcgctaga
      301 aaaatcagac ctccttgtga agattgagga gatactgaat gaaaatcaaa gaagaaacta
      361 gcaagctagt agcagattgc ccaaaacacc gctttgaggt tgtagataag actgacgtat
      421 ataatccaag gtgaagcgac tgtggtttga agagattttc aaagagtata ggctagagag
      481 tagtgttttt atgtccttct agtagaaaat gctagacaga agaatgggga acttggatag
      541 gaaaaataga ttgagaaagg aggttagaag agatgattat tacaaaaatt agccgtttag
      601 gaacttatgt gggagtaaat ccacattttg caacattaat agatttttta gaaaaaacag
      661 gactagaaaa tttaacagaa ggttcgattg ctatcgatgg taatcgattg tttgggaatt
      721 gctttactta tctagcagat ggtcaagcag gagccttctt tgaaacccac caaaaatatt
      781 tggatattca tttagtgctg gaaaacgaag aagccatggc tgtgacgtcg ccggaaaatg
      841 taagcgttac ccaagagtat gatgaagaga aggatattga attatacaca ggggaagtgg
      901 aacagttagt tcatttgaga gctggcgaat gcctcatcac ttttccagaa gatttacatc
      961 aacccaaggt tcgtataaat gatgaacctg tgaaaaaagt tgtctttaaa gttgcgattt
     1021 cttaatgtag aaagagaaga acgatgaaaa aaatgagaaa gtttttatgt ctagctggaa
     1081 ttgcgctagc ggctgttgcc ttggtagctt gttcaggaaa aaaagaagct acaactagta
     1141 ctgaaccacc aacagaatta tctggtgaga ttacaatgtg gcactccttt actcaaggac
     1201 cccgtttaga aagtattcaa aaatcagcag atgctttcat gcaaaagcat ccaaaaacga
     1261 aaatcaagat tgaaacattt tcttggaatg acttctatac taaatggact acaggtttag
     1321 caaatggaaa tgtgccagat atcagtacag ctcttcctaa ccaagtaatg gaaatggtca
     1381 actcagatgc tttggttccg ctaaatgatt ctatcaagcg tattggacaa gataaattta
     1441 acgaaactgc cttaaatgaa gcaaaaatcg gagatgatta ctactctgtt cctctttatt
     1501 cacatgcaca agtcatgtgg gttagaacag atttgttaaa agaacataat attgaggttc
     1561 ctaaaacttg ggatcaactc tatgaagctt ctaaaaaatt gaaagaagct ggagtttatg
     1621 gcttgtctgt tccgtttgga acaaatgact taatggcaac acgtttcttg aacttctacg
     1681 tacgtagtgg tggaggaagc ctcttaacaa aagatcttaa agcagacttg acaagccaac
     1741 ttgctcaaga tggtattaaa tactgggtta aattgtataa agaaatctca cctcaagatt
     1801 ctttgaactt taatgtcctt caacaagcta ccttgttcta tcaaggaaaa acagcatttg
     1861 actttaactc tggcttccat atcggaggaa ttaatgccaa cagtcctcaa ttgattgatt
     1921 cgattgatgc ttatcctatt ccaaaaatca aagagtctga taaagaccaa ggaattgaaa
     1981 cctcaaacat tccaatggtt gtttggaaaa attcaaaaca tccagaagtt gctaaagcat
     2041 tcttagaagc actttataat gaagaagact acgttaaatt ccttgattca actccagtag
     2101 gtatgttgcc aactattaag gggattagcg attctgcagc ctataaagaa aatgaaactc
     2161 gtaagaagtt taaacatgct gaagaagtaa ttactgaagc tgttaaaaaa ggtactgcta
     2221 ttggttatga aaatggacca agtgtacaag ctggtatgtt gactaaccaa cacattattg
     2281 aacaaatgtt ccaagatatc attacaaatg gaacagatcc tatgaaagca gcaaaagaag
     2341 cagaaaaaca attaaatgat ttatttgagg ctgttcagta gatgtaaaag actagaaaat
     2401 aggtgggcta gtgagctgaa aagctctagc ccaatcttgt aaaagaaggg agaaggagaa
     2461 tggttaaaga acgtaattta actcgctgga tatttgtttt gccagctatg attatcgtag
     2521 gattactctt tgtttatccg tttttctcga gtatttttta tagctttacc aataagcatt
     2581 tgattatgcc taattataaa tttgttggtt tggctaacta taaagctgtg ctatcagatc
     2641 ccaacttctt taatgcgttc tttaattcaa ttaagtggac cgttttctca ttagttggtc
     2701 aagttttagt agggtttgta ttggctttag ctcttcacag agtacgccac ttcaagaaat
     2761 tatataggac cttattgatt gttccttggg catttcctac catcgttatt gccttttctt
     2821 ggcagtggat tctaaacggg gtttatggct acttacctaa tctaatcgta aaattaggtt
     2881 taatggaaca tacacctgca tttttgacag atagtacatg ggcattccta tgtttggtgt
     2941 ttatcaacat ttggtttgga gcaccaatga ttatggttaa tgtgctttca gctttgcaaa
     3001 cagtaccaga agaacaattt gaggctgcta agatagatgg tgcttcaagt tggcaggtgt
     3061 tcaagtttat cgtctttcca catattaaag tggttgtagg acttctagtt gttttgagaa
     3121 ctgtatggat ctttaataac tttgacatta tctacctcat tactggtggt ggaccagcca
     3181 atgctacaac gacgcttcca atttttgctt acaacctggg ttggggaact aaattgttgg
     3241 gtcgtgcttc agcagttaca gtattgctct ttatcttctt ggtggcgatt tgctttatct
     3301 actttgctat catcagtaag tgggaaaagg agggtagaaa ataatgaaga agaaatccag
     3361 tatttattta gatattctct cacatgtact tttagttggt gcgaccatcg ttgcaatttt
     3421 cccattggta tggattatca tatcttctgt caaagggaaa ggggaattaa ctcagtatcc
     3481 aacacgattt tggcctgaac agtttacatt agattatttc actcatgtta tcaacgattt
     3541 gcacttcatt gataacattc gaaacagttt aatcattgcc ttggctacaa ccctcattgc
     3601 gattattatt tctgctatgg cagcctatgg tattgttcga ttctttccta aattgggagc
     3661 aatcatgtcg agactactcg tcattaccta tattttccca ccaattttgt tagcaattcc
     3721 ctattcaatt gccattgcta aagttgggtt aacaaatagt ttatttggct tgatgatggt
     3781 ttatctatct tttagtgttc catatgcagt ttggctctta gttggatttt tccaaacagt
     3841 tccaattgga attgaagaag cggctagaat tgatggtgca aataaatttg ttacgtttta
     3901 taaagttgtg ctaccgattg tagcaccagg tattgtagca acagctattt atacatttat
     3961 caatgcttgg aatgaattcc tgtatgcctt gattttgatt aacaatacag gaaagatgac
     4021 agtagcagta gcccttcgtt cacttaatgg ttcagaaata ctagactggg gagatatgat
     4081 ggcagcgtct gttattgtag ttcttccatc aattattttc ttctctatca tccaaaataa
     4141 gattgcaagt ggattatcag aaggatctgt gaagtagacg aaagaaggaa aaaaaatgaa
     4201 taaaagaggt ctttattcaa aactaggaat ttctgttgta ggcattagtc ttttaatggg
     4261 agttcccact ttgattcatg cgaatgaatt aaactatggt caactgtcca tatctcctat
     4321 ttttcaagga ggttcatatc aactgaacaa taagagtata gatatcagct ctttgttatt
     4381 agataaattg tctggagaga gtcagacagt agtaatgaaa tttaaagcag atgaaccaaa
     4441 ctctcttcaa gctttgtttg gcctatctaa tagtaaagca ggctttaaaa ataattactt
     4501 ttcaattttc atgagagatt ctggtgagat aggtgtagaa ataagagacg cccaaaaggg
     4561 aataaattat ttattttcta gaccagcttc attatgggga aagcataaag gacaggcagt
     4621 tgaaaataca ctagtatttg tatctgattc taaagataaa acatacacaa tgtatgttaa
     4681 tggaatagaa gtgttctctg aaacagttga tacatttttg ccaatttcaa atataaatgg
     4741 tatagataag gcaacactag gagctgttaa tcgtgaaggt aaggaacatt acctcgcaaa
     4801 aggaagtatt gatgaaatca gtctatttaa caaagcaatt agtgatcagg aagtttcaaa
     4861 tattcccttg tcaaatccat ttcagttaat tttccaatca ggagattcta ctcaagctaa
     4921 ctattttaga ataccgacac tatatacatt aagtagtgga agagttctat caagtattga
     4981 tgcacgttat ggtgggactc atgattctaa aagtaagatt aatattgcca cttcttatag
     5041 tgatgataat gggaaaacgt ggagtgagcc aatttttgct atgaagttta atgactatga
     5101 ggagcagtta gtttactggc cacgagataa taaattaaag aatagtcaaa ttagtggaag
     5161 tgcttcattc atagattcat ccattgttga agataaaaaa tctgggaaaa cgatattact
     5221 agctgatgtt atgcctgcgg gtattggaaa taataatgca aataaagccg actcaggttt
     5281 taaagaaata aatggtcatt attatttaaa actaaagaag aatggagata acgatttccg
     5341 ttatacagtt agagaaaatg gtgtcgttta tgatgaaaca actaataaac ctacaaatta
     5401 tactataaat gataagtatg aagttttgga gggaggaaag tctttaacag tcgaacaata
     5461 ttcggttgat tttgatagtg gctctttaag agaaaaacat aatggaaaac aggtccctat
     5521 gaatgttttc tacaaagatt cgttatttaa agtgactcct actaattata tagcaatgac
     5581 aactagtcag aatagaggag agagttggga acaatttaag ttgttgcctc cgttcttagg
     5641 agaaaaacat aatggaactt acttatgtcc cggacaaggt ttagcattaa aatcaagtaa
     5701 cagattgatt tttgcaacat atactagtgg agaactaacc tatctcattt ctgatgatag
     5761 tggtcaaaca tggaagaaat cctcagcttc aattccgttt aaaaatgcaa cagcagaagc
     5821 acaaatggtt gaactgagag atggtgtgat tagaacattc tttagaacca ctacaggtaa
     5881 gatagcttat atgactagta gagattctgg agaaacatgg tcgaaagttt cgtatattga
     5941 tggaatccaa caaacttcat atggcacaca agtatctgca attaaatact ctcaattaat
     6001 tgatggaaaa gaagcagtca ttttgagtac accaaattct agaagtggcc gtaagggagg
     6061 tcaattagtt gttggtttag tcaataaaga agatgatagt attgattgga aataccacta
     6121 tgatattgat ttgccttcgt atggttatgc ctattctgcg attacagaat tgccaaatca
     6181 tcacataggt gtgctgtttg aaaaatatga ttcgtggtcg agaaatgaat tgcatttaag
     6241 caatgtagtt cagtatatag atttggaaat taatgattta acaaaataaa ggagaaaaaa
     6301 catggttaaa tacggtgttg ttggagcagg gtattttgga gctgaattgg ctcgctacat
     6361 gcaaaagaat gatggagcag agattactct tctctatgat ccagataatg cagaggcgat
     6421 tgcagaagaa ttgggagcaa aagtagcaag ttccttagat gagttggttt ctagcgatga
     6481 agtagattgt gttatcgtcg caactccaaa taatcttcat aaggaaccgg ttattaaggc
     6541 tgcacagcat ggtaaaaatg ttttctgtga aaaaccaatt gcgctttctt atcaagattg
     6601 tcgcgagatg gtagatgcgt gtaaagaaaa caatgtaacc tttatggcag gacatattat
     6661 gaatttcttt aatggtgttc atcatgcaaa agaactcatt aatcaaggag ttatcggaga
     6721 cgttctatat tgtcatacag ctcgtaatgg ttgggaagaa caacaaccgt cagtatcatg
     6781 gaaaaaaatt cgtgaaaaat caggtggtca cttgtatcac cacatccatg aattggattg
     6841 cgttcaattc cttatggggg gcatgcctga aactgtaacc atgacaggtg gaaatgtggc
     6901 ccatgaaggt gaacatttcg gtgatgaaga tgatatgatt tttgtcaata tggaattttc
     6961 taataagcgt tttgccttgt tagaatgggg ttcagcttat cgttggggtg aacattatgt
     7021 cttaatccaa ggaagcaaag gtgccatccg cttagactta ttcaactgta aaggaactct
     7081 taagctagat gggcaagaaa gctatttctt gattcacgaa tcgcaagaag aagatgatga
     7141 tcggactcgt atctatcata gtacagagat ggatggagca attgcttatg gtaaaccagg
     7201 taaacgtact ccattatggc tatcatctgt cattgataaa gaaatgcgct atctgcatga
     7261 gattatggaa ggagccccag tatcagaaga atttgcaaaa cttttgacag gtgaagctgc
     7321 cctagaagca attgctactg cagatgcttg tacccagtct atgtttgaag atcgcaaagt
     7381 aaaattgtca gaaattgtaa aataaatttt ggtattctcc tatttatagg tcgacttgct
     7441 cctctgaaag tacttttaga ggagctgttt gactttgcta gtttttgaaa ctgaaatcta
     7501 ttatactaca aaccattgaa agcgttttaa ttttaaggta taataatctc atagaaataa
     7561 agaaaaggag gaaagaggat gccacagatt agcaaagaag ccttgattga gcaaatcaaa
     7621 gatggaatca tcgtttcttg tcaggctctt cctcatgaac cgctttatac agaagcggga
     7681 ggggtgattc ccttgctggt caaagcggct gagcaaggtg gagcagtcgg tatccgagca
     7741 aacagtgttc gcgatatcaa ggaaattaag gaagtcacta aacttccaat cattgggatt
     7801 atcaaacgtg attatccacc tcaggaaccc ttcatcacgg ctactatgaa agaagttgat
     7861 gaattggcag aactggacat cgaggtgatt gctctggatt gtaccaagcg tgaacgctac
     7921 gatggtttgg aaattcaaga gttcattcgt caggttaagg agaaatatcc taatcagctt
     7981 ttgatggctg atactagtat cttcgaagaa gggctagcag ctgtagaagc aggaattgac
     8041 tttgtcggaa caaccttatc aggctacaca tcctacagtc caaaagtaga cggtccagat
     8101 tttgaattga ttaagaaact ctgtgatgcc ggtgtagatg tcattgcaga aggaaaaatt
     8161 catacaccag aacaagccaa acaaatcctt gaatatggag tgcgaggcat cgttgttggt
     8221 ggcgccatta ctagaccaaa agagattaca gaacgcttcg ttgctagtct taaataagat
     8281 gtgagaggga gttttatgtt taaagtttta caaaaagttg gaaaagcttt tatgttacct
     8341 atagctatac ttcctgcagc aggtctactt ttggggattg gtggtgcact ttcaaaccca
     8401 accacgatag caacttatcc aatactagac aatagtattt ttcaatcaat attccaagta
     8461 atgagctctg caggagaggt tgtattcagt aatttgtcac tacttctctg tgtgggatta
     8521 tgtattggct tagcgaaacg agataaagga accgctgcgt tagcaggagt aactggttac
     8581 ttagttatga ctgcaacgat caaagctttg gtaaaacttt ttatggcaga aggatctgca
     8641 attgatactg gagttattgg agcattagtt gtcggaatag ttgccgtata tttgcacaac
     8701 cgatataaca atattcaatt accttccgct ttaggattct ttggaggttc acgcttcgtt
     8761 cctattgtta catcgttctc ttctatcttg attggctttg tcttctttgt tatttggcca
     8821 cctttccaac aacttcttgt ttctacaggt ggatatattt ctcaggcggg tccaattgga
     8881 acttttctat atggattttt aatgagactt tctggagcag taggcttaca tcatataatt
     8941 taccctatgt tttggtatac tgaacttggt ggtgttgaaa ctgttgcagg acaaacagtg
     9001 gttggagctc aaaaaatatt ttttgctcaa ttagccgatc cggcccattc tggattattt
     9061 acagaaggaa caaggttttt tgcaggtcgt ttctcaacaa tgatgttcgg tttaccggct
     9121 gcctgtttag cgatgtacca tagtgttcct aaaaatcgtc gtaaaaaata cgcgggtttg
     9181 ttttttggag ttgctttaac atcttttatt accggtatta cagaaccaat tgaatttatg
     9241 tttctattcg tcagtccggt tctatatgtt gttcacgcat tccttgatgg tgttagcttc
     9301 tttattgcag acgtcttaaa tatttcaata ggaaacacat tttcaggagg tgtaatcgat
     9361 ttcactttat ttggaatttt gcaggggaac gctaagacga attgggttct tcagattcca
     9421 attggactta tttggagtgt tttgtattat attattttta gatggttcat tactcaattc
     9481 aacgttctaa cgccaggacg aggagaagaa gtagattcta aagaaatttc tgaatccgca
     9541 gattcaactt caaatactgc agattattta aaacaggata gcctacaaat tatcagagcc
     9601 ttgggtggat caaataatat agaagatgta gatgcttgtg tgacacgttt acgtgtagct
     9661 gtaaaagaag ttaatcaagt tgataaagca cttttaaaac aaattggtgc agttgatgtc
     9721 ttagaagtga agggtggcat tcaagcaatc tatggagcaa aagcaatctt atataaaaat
     9781 agtattaatg aaattttagg tgtagatgat taagtactta ctgacttaat aaaaaacaga
     9841 ggagagtgat ggatgagtag gatgaaatga aatcgtatac aagaaataaa gaactcatta
     9901 tccaagttgg atacgcttat tacataggag aatacaaatg aaatttagaa aattagcttg
     9961 tacagtactt gcgggtgctg cggttcttgg tcttgctgct tgtggcaatt ctggcggaag
    10021 taaagatgct gccaaatcag gtggtgacgg tgccaaaaca gaaatcactt ggtgggcatt
    10081 cccagtattt acccaagaaa aaactggtga cggtgttgga acttatgaaa aatcaatcat
    10141 cgaagcgttt gaaaaagcaa acccagatat aaaagtgaaa ttggaaacca tcgacttcaa
    10201 gtcaggtcct gaaaaaatca caacagccat cgaagcagga acagctccag acgtactctt
    10261 tgatgcacca ggacgtatca tccaatacgg taaaaacggt aaattggctg agttgaacga
    10321 cctcttcaca gatgaatttg ttaaagatgt caacaatgaa aacatcgtac aagcaagtaa
    10381 agctggagac aaggcttata tgtatccgat tagttctgcc ccattctaca tggcaatgaa
    10441 caagaaaatg ttagaagatg ctggagtagc aaaccttgta aaagaaggtt ggacaactga
    10501 tgattttgaa aaagtattga aagcacttaa agacaagggt tacacaccag gttcattgtt
    10561 cagttctggt caagggggag accaaggaac acgtgccttt atctctaacc tttatagcgg
    10621 ttctgtaaca gatgaaaaag ttagcaaata tacaactgat gatcctaaat tcgtcaaagg
    10681 tcttgaaaaa gcaactagct ggattaaaga caatttgatc aataatggtt cacaatttga
    10741 cggtggggca gatatccaaa actttgccaa cggtcaaaca tcttacacaa tcctttgggc
    10801 accagctcaa aatggtatcc aagctaaact tttagaagca agtaaggtag aagtggtaga
    10861 agtaccattc ccatcagacg aaggtaagcc agctcttgaa taccttgtaa acgggtttgc
    10921 agtattcaac aataaagacg acaagaaagt cgctgcatct aagaaattca tccagtttat
    10981 cgcagatgac aaggagtggg gacctaaaga cgtagttcgt acaggtgctt tcccagtccg
    11041 tacttcattt ggaaaacttt atgaagacaa acgcatggaa acaatcagcg gctggactca
    11101 atactactca ccatactaca acactattga tggatttgct gaaatgagaa cactttggtt
    11161 cccaatgttg caatctgtat caaatggtga cgaaaaacca gcggatgctt tgaaagcctt
    11221 cactgaaaaa gcaaacgaaa caatcaaaaa agctatgaaa caatagttct tagttattct
    11281 ataaaaagta gttttttaaa gaacctaaga gtgtataccc ccttttccct ctacacagat
    11341 agtgtaagaa aagggggctt ttgtttaaaa tgtaagaaac tgtcacgaaa ttaaaatgaa
    11401 gttcttacat aagcgaatca taaaaaattt cattttgatt ttaaaacagt tcaagaaagt
    11461 caaaaaatta ttctatttga aagagaggtg ccgactgtga aagtcaataa aatccgtatg
    11521 cgggaaacag tgatttccta cgctttccta gcaccagtat tattcttctt tgtcatcttt
    11581 gtgttggctc cgatggtgat gggcttcatt acaagtttct ttaactactc aatgactaaa
    11641 tttgagtttg taggcttgga taactatatc cgtatgttta aagatcctgt ctttacaaaa
    11701 tctctgatta acacagttat tttggttatt ggatctgtac cagttgttgt tctattctca
    11761 ctctttgtag catctcagac ctatcatcaa aatgtcattg ccagatcctt ctaccgtttc
    11821 gtcttcttcc ttcctgttgt aacgggtagt gttgccgtga cagttgtttg gaaatggatt
    11881 tatgacccac tatcagggat tctaaacttt gtccttaagt ccagccacat catcagccaa
    11941 aacatttctt ggttgggaga taaaaactgg gcattgatgg cgattatgat tattctcttg
    12001 accacttcag ttggtcagcc catcatcctt tatatcgctg ccatggggaa tattgacaat
    12061 tcactggttg aagcggcgcg tgttgatggt gcaactgagt ttcaagtttt ttggaagatt
    12121 aaatggccaa gccttcttcc aacaactctt tatattgcaa tcatcacaac aattaactca
    12181 ttccagtgtt tcgccttgat tcagcttttg acatctggtg gtccaaacta ctcaacaagt
    12241 accttgatgt actaccttta cgaaaaagcc ttccaattga cagaatacgg ctatgccaac
    12301 acaattggtg tcttcttggc agtcatgatt gctatcgtaa gctttgttca atttaaagta
    12361 cttggaaacg acgtagaata ctaaagaaag gagacagcta tgcaatctac agaaaaaaaa
    12421 ccattaacag cctttactgt tatttcaaca atcattttgc tcttgttgac tgtgctgttc
    12481 atctttccat tctactggat tttgacaggg gcattcaaat cacaacctga tacaattgtt
    12541 attcctcctc agtggttccc taaaatgcca accatggaaa acttccaaca actcatggtg
    12601 cagaaccctg ccttgcaatg gatgtggaac tcagtattta tctcattggt aaccatgttc
    12661 ttagtttgtg caacctcatc tctagcaggt tatgtattgg ctaaaaaacg tttctatggt
    12721 caacgcattc tatttgctat ctttatcgct gctatggcgc ttccaaaaca agttgtcctt
    12781 gtaccattgg tacgtatcgt caacttcatg ggaatccacg atactctctg ggcagttatc
    12841 ttgcctttga ttggatggcc attcggtgtc ttcctcatga aacaatttag tgaaaatatt
    12901 cctacagagt tgcttgaatc agctaaaatc gacggttgtg gtgagattcg taccttctgg
    12961 agtgtagcct tcccgattgt gaaaccaggg tttgcagccc ttgcaatctt taccttcatc
    13021 aatacttgga atgactactt catgcaattg gtaatgttga cttcacgtaa caatttgacc
    13081 atctcacttg gggttgcgac catgcaggct gaaatggcaa ccaactatgg tttgattatg
    13141 gcaggagctg cccttgctgc tgttccaatc gtcacagtct tccttgtctt ccaaaaatcc
    13201 ttcacacagg gtattactat gggagcggtc aaaggataat actctgcgaa aatctcttca
    13261 aactacgtca gcttcacctt gccatactta agtattgcct gcggttagct tcctagtttg
    13321 ttcttcaatt ttcattgagt ataggaaaat caatctatca agatacagaa gtatatttta
    13381 tagatttaga gaatatagag gttataagtg tctacaaaat ggagggtatg cagttacttt
    13441 atgaagtttt gtcagacact tataaactta agaatggttt tagttaacta tcagaaacga
    13501 aggaaagagt atgatttttg acgatttgaa aaacatcacc ttttacaaag ggattcatcc
    13561 taatttagac aaggctatcg actatctcta ccaacatcgt aaggattctt tcgaattagg
    13621 aaagtatgat attgatggag ataaagtctt tctagttgtt caggaaaatg tcctcaatca
    13681 agctgaaaat gatcaatttg agtatcataa gaactatgca gatttgcatt tgctggtaga
    13741 aggacatgaa tattcgagct acggttcacg tatcaaagac gaggcagtag cattcgacga
    13801 agcgagtgac attggctttg ttcattgtca tgaacactac ccactcttgt tgggttatca
    13861 caattttgcg attttcttcc caggtgagcc acatcagcca aatggttatg caggcatgga
    13921 agaaaaggtt cgaaaatatc tctttaaaat tttgattgat taaaaatagg atgaattgtt
    13981 tttttgtaaa gctttgataa tactctacca tgaaattgat ctttgtgagg tagagaaatg
    14041 agaataaaat atttaaaaat tggtatcttc ttgtcagtct gtgtatgcta caagagctag
    14101 tttcttggat ggacagggaa ttacagttga tgagatggct tggataatta ggggcattgt
    14161 gaatgcattg attggtagat acataaaatt aggtacttat gcggctaagt atggtattag
    14221 tatggcacgc tcgatcttaa gtagggcagc tgcaactgca gcagcaagag taggattatc
    14281 gaccaagatt tctggatgga ttttacgagt agctgtgaat gtagctgatg tatatggtaa
    14341 ttttgccaac aatattgctg cagcttggga tgcacatgat aaaatcccta acaatggtcg
    14401 tataaacttt taaaatgcga gaatgaaagc actttgtatt tttttattga atatgttagc
    14461 ttggacagtg cttgcaatga taattcgtgg agggctagat ggatttgatg agcatacttg
    14521 gagtactatt ttaattgctt cgctgttcgg ggtatatgat tataagccca tagataaaaa
    14581 tagaaaaagt ccaaaagaaa aaagaaaaaa tagatttgtt catggtaggg atttatgaaa
    14641 gctttactga caaaaaagaa aacagtttac aaagaaaaat gatggaggag caaacatggc
    14701 acaaaaagga gtaagcctta tcaaggcagc atttgataca gacaactttc tcatgcgttt
    14761 tagtgagaaa gtcttggaca tcgtgacagc caatcttctt tttgtcgtct cttgtttgcc
    14821 catcgtgacg attggagtag ctaaaatcag cctctacgag accatgttcg aagttaagaa
    14881 gagcagacgg gtgcctgttt ttaaaatcta tctaagatct ttcaagcaaa atctgaaact
    14941 aggtcttcag ctgggtttaa tggagttagg aattgtgttt cttacccttt cagatctcta
    15001 tcttttctgg ggtcaaacag ctctgccctt ccaattgctg aaagccattt gtttaggtat
    15061 tctgattttt cttactatcg tgatgctggc tagttaccct atcgcggcac gttatgacct
    15121 atcttggaaa gaagtgttac aaaaagggct tatcttggca agttttaact ttccttggtt
    15181 cttcctcatg ttagccattc ttgtcctcat tgtgatggtt ctttatctgt ctgccttcag
    15241 tctactctta ggtggctcag ccttcctact ttttgggttt ggacttcttg cttttttaca
    15301 agtgggagtg atggaaaagc tatttgctaa gtatgagtaa ggggtgcaat tatttctgaa
    15361 actactttca aaggctccaa acgctattct ataagcgaga aactaaaatc taagtgtcta
    15421 taagatattg aaagcctttt cggtaaagtg tatactaaag aagtaaagaa aaatctgctt
    15481 tagcagaaaa taaaaataat aggagaaaat ctatgtcaga tttgaaaaaa tacgaaggtg
    15541 tcattccagc cttctacgca tgttatgatg atcaaggaga agtaagccca gaacgtacgc
    15601 gtgccttggt tcaatacttc attgataaag gtgttcaagg tctttatgtc aatggttctt
    15661 ctggtgaatg tatctaccaa agcgttgaag atcgcaagtt gattttggaa gaagtcatgg
    15721 cggtagccaa aggtaaattg accattattg cccatgttgc ttgcaataat actaaagata
    15781 gtatggaact tgctcgccat gctgaaagct tgggagtaga tgctattgca acgattccac
    15841 caatttattt ccgcttgcca gaatactcag ttgccaaata ctggaacgat atcagttctg
    15901 cagctccaaa cacagactac gtgatttaca acattcctca attggcaggg gttgctttga
    15961 ctccaagcct ttacacagaa atattgaaaa atcctcgtgt tatcggtgtg aagaactctt
    16021 ctatgccagt tcaagatatc caaacctttg tcagccttgg tggagaagac catatcgtct
    16081 ttaatggtcc tgatgagcag ttcctaggtg gccgcctcat gggtgctaag gctggtatcg
    16141 gtggtactta tggtgctatg ccagaactct tcttgaaact caatcagttg attgcggata
    16201 aggacctaga aacagcgcgt gaattgcagt atgctatcaa cgcaatcatt ggtaaactca
    16261 cttctgctca tggaaatatg tacggtgtca tcaaagaagt cttgaaaatc aatgaaggct
    16321 tgaatattgg atctgttcgt tcaccattga caccagtaat tgaagaagat cgtccagttg
    16381 tagaagcggc tgcagccttg attcgtgaaa ccaaggagcg cttcctctaa tctaaaagga
    16441 ggtatttatg acatactacg ttgcaattga tatcggtgga actaacatca agtatggttt
    16501 ggttgatcaa gaggggcaac ttcttgaatc acatgaaatg ccaacagagg cgcataaggg
    16561 tggacctcat atcttacaaa agaccaaaga tatcgtagct agttatttag aaaaaggccc
    16621 agtagcaggt gttgccatat cttctgctgg gatggtggat ccggataagg gtgagatttt
    16681 ctatgctggg ccgcaaatcc ctaactacgc aggcacccag ttcaaaaagg aaatcgaaga
    16741 aagctttact attccttgtg agattgaaaa tgatgtcaac tgtgcaggtc ttgctgaggc
    16801 agtatctggt tcaggcaagg gagcaagtgt gacactttgc ttgaccattg gaaccggtat
    16861 cggtggttgc ttgattatgg ataggaaagt cttccatggt tttagtaatt cagcctgtga
    16921 agtcgggtat atgcatatgc aggatggagc ttttcaggat ctcgcttcta caacagcttt
    16981 agtgaaatat gtagctgaag cccatggaga agatgttgat cagtggaatg gccgacgtat
    17041 ctttaaggaa gctactgaag gaaacaaaat ctgcatggaa ggtattgacc gtatggttga
    17101 ctatctagga aaaggtctgg caaatatttg ctacgttgcc aatccagaag tggtcattct
    17161 tggtggtggt atcatggggc aagaggctat cctcaaacct aagatccgca cagccttgaa
    17221 agaggctttg gtaccaagtt tagcagaaaa aacacgatta gaatttgccc atcaccaaaa
    17281 tacagcaggg atgttgggtg catattatca ttttaagaca aaacaatcct agtttggctc
    17341 agccaaacta ggattttctt acacgttttt gtctacgata gccgttgagt tttttatttt
    17401 cccagtagct attaaagatt ttttccttgc tttcgcgatt gatttccaaa aagtaggcat
    17461 aaatcaaatc gataaagaag agcataggaa gttgagcgga tattcgttgg atataggagg
    17521 gttggctgtg ggtggctaca agaacagtct ctgtataggt ctggctatct ttattgggaa
    17581 cacttgtaaa gagtacagtc tttgccccca tctccttggc atctaataga ctatctaaaa
    17641 tagaaggagt tgagcctgaa agtgagaagc ccagtactag acaattttca tccatgatgc
    17701 tggttgtcca ggcaaagccg tcttggtctg tcaaagcttc gcagaccaca cctaatcgca
    17761 taaaacgtaa tttcatttca cgggcgacga ggccagaact ccctgttcca aagaagtaga
    17821 tacgctcagc atcttcgatt agctgggcaa ttcgttctag ttggatttcg tcaatcaagt
    17881 cttgtgtttg ttccctcata ttgctataac ttctgaggac tcgtttggtc agtggactgt
    17941 gcttggagac ttggttggct tgattttctg cctgatgttg gtattggaaa ataaattctc
    18001 ggtagccagt aaagccacac tttttagcaa agcgggtcaa agcagcttga gaaatatgta
    18061 atttttgggt gacttgttga gaagataaat catctgtaat cgtttcagct tgcaaaaaat
    18121 agcgagcgat ttcttgctct aggtctgtca tttcttcaaa atgtgaatca atgatagttg
    18181 cgatatctgg tttgtccata gggaaagctc ctttacatga gtcatactgg aagactagat
    18241 cagggaatag tcacacttca ttataacaca taatataagg atagataaat aaaaacgcat
    18301 ctctgtttta aaaacgaaaa aatcgaaaaa gcttctctct tttccataat tttctactca
    18361 aattgtggta caattaagag taagatttta agttagaaat gagactgatt tgtatgagaa
    18421 aatttaacag ccattcgatt ccgattcggc ttaatttatt gttttcaatc gtcattttac
    18481 tctttatgac cattattggt cgtttgttgt atatgcaggt tttgaacaag gatttttacg
    18541 aaaaaaagct agcctcagct agtcagacca agattacaag cagttcagcc cgtggggaaa
    18601 tttatgatgc tagtggaaaa cctttggtag aaaatacgtt aaagcaggtt gtttccttta
    18661 cgcgtagcaa taaaatgacg gctacagact taaaagaaac agctaaaaag ttactgactt
    18721 atgtgagcat cagttctcca aatttgacag aacgccagct ggcggattac tatttggctg
    18781 atcctgaaat ctataaaaaa acagtggaag ctctcccaag cgagaaacgc ttggattcag
    18841 atggcaatcg tctatctgaa tcagaactgt ataacaatgc ggtcgatagt gtacaaacga
    18901 gtcaactaaa ctatacagag gatgaaaaga aagaaatcta tctttttagt cagttaaatg
    18961 ctgttggaaa ctttgcgaca ggaaccattg cgacagatcc tctaaatgat tctcaggtgg
    19021 ctgttattgc ctctatttca aaggagctgc ctggcattag tatttctact tcttgggata
    19081 gaaaggtttt ggaaacttcc ctttcttcta tagttgggag tgtatccagt gaaaaagctg
    19141 gtctcccagc ggaagaagca gaagcctatc ttaaaaaagg ctattctcta aatgatcgtg
    19201 taggaacctc ctatttggaa aagcaatatg aagagacctt acaaggaaaa cgctcggtaa
    19261 aagaaatcca tctggataaa tatggcaata tggaaagcgt ggatacaatt gaggaaggta
    19321 gtaagggaaa caatatcaaa ctgaccattg atttggcctt ccaagatagc gtggatgctt
    19381 tactgaaaag ttatttcaat tccgagctag aaaatggtgg agccaagtat tctgaaggtg
    19441 tctatgcagt cgcccttaac ccaaaaacag gtgcggtttt gtctatgtca gggattaaac
    19501 atgacttgaa aacgggagag ttgacgcctg attccttggg aacggtaacc aatgtctttg
    19561 ttccaggttc ggttgtcaag gcggcgacca tcagctcagg ttgggaaaat ggagtcttgt
    19621 caggaaacca gaccttgaca gaccagtcca ttgtcttcca aggttcagct cccatcaatt
    19681 cttggtatac tcaggcttac ggttcattcc ctatcacagc ggtccaagct ctggagtatt
    19741 catcaaatac ctatatggtc caaacagcct taggtcttat ggggcaaacc tatcaaccca
    19801 atatgtttgt cggcaccagc aatctagagt ctgctatgga gaaattgcgt tcaacctttg
    19861 gcgaatatgg cttgggtact gcgacaggaa ttgacctacc agatgaatct actggatttg
    19921 ttcccaaaga gtatagcttt gctaattaca ttactaatgc ctttgggcag tttgataact
    19981 atacgccgat gcagttggct cagtatgtag caactattgc aaataatggt gttcgtgtgg
    20041 ctcctcgtat tgttgaaggc atttatggta ataatgataa gggaggactg ggtgacttga
    20101 ttcagcaact gcaaccgaca gagatgaata aggtcaatat atccgactcc gatatgagca
    20161 tcttgcacca aggtttttat caggttgccc atggtactag tggattgaca actggacgtg
    20221 ccttttcaaa tggtgccttg gtatccatta gcggaaaaac aggtacagcc gaaagctatg
    20281 tggcagatgg tcagcaagca accaatacca atgcggtggc ctatgcccca tctgataatc
    20341 cccaaatcgc tgtcgcagtg gtctttcctc ataataccaa tctaacaaat ggtgtaggac
    20401 cttccattgc gcgtgacatt atcaatctgt atcaaaaata ccatccaatg aactagaaag
    20461 gaaattatgc tttatccaac acctattgcc aagttgattg acagttattc taagttacca
    20521 ggtatcggga ttaagacggc tacgcgtctg gccttttata cgattgggat gtctgctgat
    20581 gatgtcaatg aatttgcaaa aaatctcctt tctgctaaga gagaattgac atattgttct
    20641 atttgtggac gtttgacaga cgacgatcct tgttctatct gtactgatcc gactcgtgac
    20701 cagacaacaa ttttagttct tgaggatagt agagatgtgg cagccatgga aaatatccaa
    20761 gaataccatg gactctatca tgtcctgcat ggcctcattt ctcctatgaa tggtatcagt
    20821 ccggacgata tcaatctcaa gagccttatg actcgtctta tggatagtga ggtttcagaa
    20881 gtgattgtgg cgactaatgc tacagcggat ggtgaagcga cttccatgta tctttcacgt
    20941 ttgctcaagc cggctggtat caaggttacg cgtctagcac gaggtctcgc tgtgggagcg
    21001 gacattgagt atgcggacga agtgacactc ttacgagcga ttgaaaatcg gacagagttg
    21061 taagtgtagg caaatttacg aactcccttc atttataaaa aatcaaagag gctgaaaatc
    21121 gttcctatcg gcctcttttt gtatagtgtg atgagtagga tcgggttcaa gttttaaaaa
    21181 accaagcaaa tatgatatac taaagagcga gtattctagt agaattagga caaataatat
    21241 gaaacaaacg attattcttt tatatggtgg acggagtgcg gaacgcgaag tctctgtcct
    21301 ttcagctgag agtgtcatgc gtgcggtcaa ttacgaccgt ttcacagtca agactttctt
    21361 tatcagtcag tcaggtgact ttatcaaaac acaggaattt agtcatgctc cggggcaaga
    21421 agaccgtctc atgaccaatg aaaccattga ttgggataag aaagttgcac caagtgctat
    21481 ctacgaagaa ggtgcagtgg tctttccagt ccttcacggg ccaatgggag aagatggctc
    21541 tgttcaagga ttcttggaag ttttgaaaat gccttacgtt ggttgcaaca ttttgtcatc
    21601 aagtcttgcc atggataaaa tcacgactaa gcgtgttctg gaatctgctg gtattgccca
    21661 agttccttat gtggctatcg ttgaaggcga tgatgtgact gctaaaatcg ctgaagtgga
    21721 agaaaaattg gcttatccag tcttcactaa gccgtcaaac atggggtcta gtgtcggtat
    21781 ttctaagtct gaaaaccaag aagaactccg tcaagcctta aaacttgcct tccgatatga
    21841 cagccgtgtc ttggttgagc aaggagtgaa tgcccgtgaa attgaggttg gcctcttggg
    21901 taactacgat gtcaagagca cgctacctgg agaagttgtc aaggacgttg ccttttatga
    21961 ctacgatgcc aagtatattg ataacaagat tactatggat attcctgcca aaatcagtga
    22021 tgatgtggtg gctgtcatgc gtcaaaatgc agaaacagcc ttccgtgcca ttggtggcct
    22081 tggtctatct cgttgcgatt tcttctatac agataaggga gagatttttc tcaacgagct
    22141 caataccatg ccaggtttta cccagtggtc tatgtaccca ctactttggg acaatatggg
    22201 gatcagctac ccaggactaa tcgagcgttt ggttgacctt gccaaggaaa gctttgacaa
    22261 gcgcgaagcg catttgatat aaaatgaaag agagggtaga agccagaacc atcactgcac
    22321 ggtgactaga gttctcggac ttcagccctt tttaaaggag tagaaatgaa attaacaatc
    22381 catgaaattg cccaagttgt tggagccaaa aatgatatca gtatctttga ggacacccag
    22441 ttagaaaaag ctgagtttga tagtcgtttg attggaactg gagatttatt tgtgccactt
    22501 aaaggtgcgc gtgatggcca tgactttatt gaaacagcct ttgaaaatgg tgcagcagta
    22561 accttgtctg agaaagaggt ctcaaatcat ccttacattt tagtagatga tgttttgaca
    22621 gcctttcaat ccctagcatc ctactatctt gaaaaaacga ctgttgatgt ctttgctgtt
    22681 acaggttcaa atggcaagac aacgactaag gatatgttgg cgcatttact gtcaacaaga
    22741 tacaagacct acaaaacaca aggcaattac aataatgaga ttggccttcc ttacacagtt
    22801 ctccacatgc ctgaaggaac agaaaagttg gtcttggaga tgggacagga tcacttgggc
    22861 gatattcatc tcttgtctga attggctcgt ccaaaaacag ccatcgtgac cttggttgga
    22921 gaagcccatt tggccttttt caaagaccgt tcagagattg ctaagggaaa aatgcaaatt
    22981 gcagacggaa tggcttcagg ttccttgctt ttagcgccgg ctgaccctat cgtagaggac
    23041 tatttgccaa ctgataaaaa ggtggttcgt tttgggcaag gggcagagct ggaaattact
    23101 gacttggttg agcgcaaaga tagtctgacc ttcaaggcca atttcttaga gcaagccctt
    23161 gatttgccag taactggcaa gtacaatgcg acaaatgcta tgattgcatc ctatgttgcc
    23221 ttgcaagaag gagtttcaga ggagcaaatt cgtttggcct tccaagatct tgaattgacg
    23281 cgtaaccgta ccgagtggaa gaaagcagcc aatggagcag atatcctgtc agatgtttac
    23341 aatgccaatc caactgctat gaaactgatt ttagagactt tctctgccat tccagccaat
    23401 gaaggtggca agaaaattgc agtgttggcg gatatgaagg agcttggtga ccagtctgtt
    23461 caacttcata atcagatgat tttgagcctt tctccagatg tgcttgatac cgtgattttc
    23521 tatggacaag atattgctca gttggctcaa ttggccagtc aaatgttccc aatcggccac
    23581 gtttactact tcaagaaaac agaagaccag gatcaatttg aagacctagt caagcaggtc
    23641 aaggaaagcc ttggagccca tgaccaaatc ctgctcaaag gctctaactc tatgaatcta
    23701 gccaagttgg tagaaagttt agaaaatgaa gacaagtgat tttgtcaagt atttgcaaag
    23761 aatgattgcc attacagata ctggcttaac ctttacaaaa gatccgtttg accgtgagcg
    23821 ctacgaagac ttgcgaagtc tgttatctga aatgttgaat caagcatcag accttgattc
    23881 cgaagaagtg gcagaagtct tgaagccaac ttctgcttat gcgactccct taatggacgt
    23941 ccgtgcttgg attgttgagg atgagaagat ttgtctggtt aggggacaag gagaggatag
    24001 ttgggctttg ccaggtggct ttggtgaagt cggctattct ccaacagaaa atattctcaa
    24061 ggaaattgaa gaagaaaccg gttttaaagc caaagttgaa agactgctag ctgtttttga
    24121 taccaatcgt ttccaactac agagcaaaca gtatacaaag tttgtttttg gatgtaagct
    24181 tcttgatgga caattccaag aaaatcaaga aattgctgac cttcaatttt ttgccattga
    24241 ccaactgccg aacttatctg aaaaacgcat taccaaggag caaatagagc ttctttggca
    24301 ggtttatcaa ggtcataggg ggcaatatct tgactaagaa gatgattatc gtatttctaa
    24361 atccattttt aacaactagc atggtataat aatatgcagg aaaattttga attatgagga
    24421 agactagatg aatttatggg atattttctt tacgactcag gcaaccgagc cgcccaaatt
    24481 tgaccttttt tggtatgtta gcctatttac gctcttagcc ttaacctttt atacagccca
    24541 tcgctatcgt gaaaagaagg tttaccaacg atttttccaa atcttgcaga ctgttcagtt
    24601 aatccttctt tatggttggt actgggtcaa tcatatgcca ctgtcagaaa gcctaccctt
    24661 ttaccattgc cgtatggcta tgtttgtggt actcttgctt cctggtcaat ccaaatataa
    24721 acaatacttt gcattattgg gaacatttgg gacattagca gcctttgttt atccagtgcc
    24781 agatgcttac ccttttccac atatcaccat tctatccttt atctttggtc atttagcact
    24841 cttggggaac tctctagttt atctattgag acagtataat gcgcgattgc tggatgtgaa
    24901 gggaattttt ctcatgacct ttgccctaaa tgccttgatt tttgtggtca atttggtgac
    24961 aggtggcgat tacggatttt tgacaaaacc gccattggtt ggggatcacg gtctagtagc
    25021 taattattta cttgtttcaa ttgtgctggt agctactatc agtttgacta agaaaatctt
    25081 agaattcttt ttagctcaag aagcagaaaa aatgattgca aaggaagctt aacacagagc
    25141 tttctttttt gctcttagag agtttttaca agcagcttat aaaataagaa tttctgaata
    25201 gacaaactca aaaaatggct gggaaattta ggaaaaaagc aagcacgatt aaattttttg
    25261 tgttataata ttttgtgaat agctatgcct atgtttagct atggaataat acgaagtgcg
    25321 aaacttggaa gatagagagg aagcgatgta atggctagag aaggcttttt tacaggtcta
    25381 gatattggaa caagctctgt caaggtgctt gtggccgagc agagaaatgg tgaattaaat
    25441 gtaattggcg tgagtaatgc caaaagtaaa ggtgtaaagg atggaattat tgttgatatt
    25501 gatgcagcag caactgctat caagtcagcc atttcccaag cggaagaaaa ggcaggcatt
    25561 tcgattaaat cagtgaatgt cggcttgcct ggtaatcttt tgcaggtaga accaactcag
    25621 gggatgattc cagtaacatc tgatactaag gaaattacgg atcaagatgt tgaaaatgtt
    25681 gtcaaatcag ctttgacaaa gagtatgaca cctgaccgtg aagtcattac ctttattcct
    25741 gaagaattta ttgtggatgg tttccaaggg attcgtgacc cacgtggcat gatgggggtt
    25801 cgccttgaaa tgcgtggttt gctttataca ggacctcgta ctatcttgca caatttgcgt
    25861 aagacggttg agcgtgcagg tgttcaggtt gaaaatgtta tcatttcacc actagcaatg
    25921 gttcagtctg ttttgaacga aggggaacgt gaatttggtg ctacagtgat tgatatgggg
    25981 gcaggtcaaa cgactgtcgc tacaatccgt aatcaagaac tccagttcac acatattctc
    26041 caagaaggtg gagattatgt aactaaagat atctccaagg ttttgaaaac ctctcgcaaa
    26101 ttagcggaag gcttgaaact gaattacggg gaagcctatc cgcctcttgc aagcaaagaa
    26161 accttccaag tagaggttat tggagaagta gaagcagtcg aagtgacgga agcctacttg
    26221 tcagaaatta tttctgcacg aatcaagcac atccttgaac aaatcaagca agaattagat
    26281 agaaggcgtc tattggacct ccctggtggt attgtcttaa tcggtgggaa tgccatttta
    26341 ccaggtatgg ttgagcttgc tcaggaagtc tttggcgtcc gtgtcaagct ttatgttcca
    26401 aatcaagttg gtatccgtaa tccagccttt gcgcatgtga ttagtttatc agaatttgcg
    26461 ggtcaattaa cagaagttaa tcttttggct cagggagcga taaaaggtga gaatgactta
    26521 agtcatcagc caattagttt tggtgggatg ctgcaaaaaa cagctcagtt tgtacaatca
    26581 acgcctgttc aaccagctcc tgctccagaa gtagagccgg tggcgcctac agaaccaatg
    26641 gcggatttcc aacaagcttc acaaaataaa ccgaaattag cagatcgttt ccgtggattg
    26701 atcggaagca tgtttgacga ataaagagga aaaataaatt atgacatttt catttgatac
    26761 agctgctgct caaggggcag tgattaaagt aattggtgtc ggtggaggtg gtggcaatgc
    26821 catcaaccgt atggtcgacg aaggtgttac aggcgtagaa tttatcgcag caaacacaga
    26881 tgtacaagca ttgagtagta caaaagctga gactgttatt cagttgggac ctaaattgac
    26941 tcgtggtttg ggtgcaggag gtcaacctga ggttggtcgt aaagccgctg aagaaagcga
    27001 agaaacactg acggaagcta ttagtggtgc cgatatggtc ttcatcactg ctggtatggg
    27061 aggaggctct ggaactggag ctgctcctgt tattgctcgt atcgccaaag atttaggtgc
    27121 gcttacagtt ggtgttgtaa cacgtccctt tggttttgaa ggaagtaagc gtggacaatt
    27181 tgctgtagaa ggaatcaatc aacttcgtga gcatgtagac actctattga ttatctcaaa
    27241 caacaatttg cttgaaattg ttgataagaa aacaccgctt ttggaggctc ttagcgaagc
    27301 ggataacgtt cttcgtcaag gtgttcaagg gattaccgat ttgattacca atccaggatt
    27361 gattaacctt gactttgccg atgtgaaaac ggtaatggca aacaaaggga atgctcttat
    27421 gggtattggt atcggtagtg gagaagaacg tgtggtagaa gcggcacgta aggcaatcta
    27481 ttcaccactt cttgaaacaa ctattgacgg tgctgaggat gttatcgtca acgttactgg
    27541 tggtcttgac ttaaccttga ttgaggcaga agaggcttca caaattgtga accaggcagc
    27601 aggtcaagga gtgaacatct ggctcggtac ttcaattgat gaaagtatgc gtgatgaaat
    27661 tcgtgtaaca gttgttgcaa cgggtgttcg tcaagaccgc gtagaaaagg ttgtggctcc
    27721 acaagctaga tctgctacta actaccgtga gacagtgaaa ccagctcatt cacatggctt
    27781 tgatcgtcat tttgatatgg cagaaacagt tgaattgcca aaacaaaatc cacgtcgttt
    27841 ggaaccaact caggcatctg cttttggtga ttgggatctt cgccgtgaat cgattgttcg
    27901 tacaacagat tcagtcgttt ctccagtcga gcgctttgaa gccccaattt cacaagatga
    27961 agatgaattg gatacacctc catttttcaa aaatcgttaa gtaaatgaat gtaaaagaaa
    28021 atacagaact tgtttttcga gaagttgcag aggctagtct gagtgctcat cgagagagtg
    28081 gttcggtctc tgtcattgca gttaccaagt atgtagatgt accgacagcg gaagccttgc
    28141 ttccgctagg tgtccatcat atcggtgaaa atcgtgtaga taagtttctg gaaaaatatg
    28201 aagctttaaa agatcgagat gtgacttggc atttgattgg taccttgcaa agacgtaagg
    28261 tgaaagatgt cattcaatac gttgattatt tccatgcatt ggactcagta aagttagcag
    28321 gggaaattca aaaaagaagt gaccgagtca tcaagtgttt ccttcaagta aatatttcta
    28381 aagaagaaag caaacacggt ttttcgagag aggaactgct ggaaatcttg ccagagttag
    28441 ccagactaga taagattgaa tatgttggtt taatgacgat ggcacctttt gaggctagca
    28501 gtgagcagtt gaaagagatt ttcaaggcgg cccaagattt acaaagagaa attcaagaga
    28561 aacaaattcc aaatatgcct atgaccgagt taagtatggg aatgagtcgt gattataaag
    28621 aagcgattca attcggttcc acttttgttc gtataggtac atcatttttt aagtaggaga
    28681 gaaccatgtc tttaaaagat agattcgata gatttataga ttattttacg gaggatgagg
    28741 attcaagtct cccttatgaa aaaagagatg agcctgtgtt tacttcagta aattcttcac
    28801 aggaaccggc tctcccaatg aatcaacctt cacagtcggc tggcacaaaa gagaacaata
    28861 tcaccagact tcatgcaaga caacaggaat tggcaaatca gagtcagcgt gcaacggata
    28921 aggtcattat agatgttcgt tatcctagaa aatatgagga tgcaacagaa attgttgatt
    28981 tattggcagg aaacgaaagt atcttgattg attttcagta tatgacagag gtgcaggctc
    29041 gtcgttgttt ggactatttg gatggagctt gtcatgtttt agctggaaat ttgaaaaagg
    29101 tagcttctac catgtatttg ttgacaccag tgaacgttat tgtaaatgtt gaagatatcc
    29161 gtttaccaga tgaagatcaa cagggtgagt tcggttttga tatgaagcga aatagagtac
    29221 gataatgatt tttttaattc gtatgattta taatgcagtg gatatttact ccctaatttt
    29281 ggtagccttc gctgtcatgt cttggtttcc aggtgcctac gaatccagtt taggtcgttg
    29341 gattgtagcg ttggtaaaac cagtgcttgc tcccttgcaa cgcctgcctt tacagatagc
    29401 gggtcttgat ttatctgttt gggttgcgat tgttttggtt cgatttttag gagaaaacct
    29461 agtgcgtttt ctggcgatga taggatgaat aaagggattt atcagcattt ctccatagaa
    29521 gatcgtccat ttcttgacaa gggaatggaa tggataaaga aggtagaaga tagctatgct
    29581 ccttttttaa ctccttttat caatcctcat caggagaagc tattaaagat tttggccaaa
    29641 acctatggtc ttgcttgtag cagtagtggg gaattcgtct cgagtgagta tgttcgagtt
    29701 ttattatacc cagattattt ccaaccagag ttttcagatt ttgaaatatc tctccaggaa
    29761 attgtgtatt ccaataaatt tgaatattta acgcatgcta agattttagg gacagtcatc
    29821 aatcaattag ggattgaacg gaaacttttt ggagatatcc tagtagatga agaacgggcg
    29881 cagattatga ttaatcagca gtttcttctc ctctttcaag atggattaaa gaaaattggt
    29941 cgtatacctg tttcgctgga ggaacgtcct ttcaccgaga aaatagataa gctagaacag
    30001 tatcgagaac tggatttatc tgtgtctagt tttcgattag atgttctttt atcaaatgtt
    30061 ttgaaactat ctaggaatca agcaaaccag ttgattgaaa agaaacttgt ccaagtaaat
    30121 tatcatgtgg tagacaaatc agattacact gttcaagttg gagacttgat tagtgtgaga
    30181 aaatttggtc gcttgagatt acttcaagat aagggacaaa cgaaaaaaga gaagaaaaaa
    30241 ataaccgtcc agttattatt aagtaagtga ggaatagaat gccaattaca tcattagaaa
    30301 taaaggacaa gacttttgga actcgattca gaggttttga tccagaagaa gtcgatgaat
    30361 ttttagatat tgtggttcgt gattacgaag atcttgtgcg tgcgaatcat gataaaaatt
    30421 tgcgtattaa gagtttagaa gagcgtttgt cttactttga tgaaataaaa gattcattga
    30481 gccagtctgt attgattgcc caggatacag ctgagcgagt gaaacaggcg gcgcatgaac
    30541 gttcaaacaa tatcattcat caagcagagc aagatgcgca acgcttgttg gaagaagcta
    30601 aatataaggc aaacgagatt cttcgtcaag caactgataa tgctaagaaa gtcgctgttg
    30661 aaacagaaga attgaagaac aagagccgtg tcttccacca acgtctcaaa tctacaattg
    30721 agagtcagtt ggctattgtt gaatcttcag attgggaaga tattctccgt ccaacagcta
    30781 cttatcttca aaccagtgat gaagccttta aagaagtggt tagcgaagta cttggagaac
    30841 cgattccagc tccaattgaa gaagaaccaa ttgatatgac acgtcagttc tctcaagcag
    30901 aaatggaaga attacaagct cgtattgagg tagccgataa agaattgtct gaatttgaag
    30961 ctcagattaa acaggaagtg gaaactccaa ctcctgtagt gagtcctcaa gttgaagaag
    31021 agcctctgct catccagttg gcccaatgta tgaagaacca gaagtagctc cagtgcatcc
    31081 gacaggtcca acaccagcta cagaaactgt tgattcagca ccgggatttg aagcaccgca
    31141 agaatctgtt acaattttat aagaaatatt ctgagaacaa tatcttatcc ttatattccc
    31201 agcgagcagg agatggtgtg agtcctgcat tccctatcga taagattatc ctctcaaact
    31261 atcaagtctg aatctagtaa gatttgacgt tccccacgtt acgggataag agggagaaag
    31321 actaaaactt tttccgaata aaggtggtac cacgattttc gtcctttttg gaagtcgtgg
    31381 tttttaattt gttattattt ataaaggaga taccatgaaa ctcaaagaca cccttaatct
    31441 tgggaaaact gaattcccaa tgcgtgcagg ccttcctacc aaagagccag tttggcaaaa
    31501 ggaatgggaa gatgcaaaac tttatcaacg tcgtcaagaa ttgaaccaag gaaaacctca
    31561 tttcaccttg catgatggcc ctccatacgc taacggaaat atccacgttg gacatgctat
    31621 gaacaagatt tcaaaagata ttattgttcg ttctaagtct atgtcaggat tttacgcacc
    31681 atttattcct ggttgggata ctcatggtct gccaatcgag caagtattgt caaaacaagg
    31741 tgtcaaacgt aaagaaatgg acttggttga gtacttgaaa ctttgccgtg agtacgctct
    31801 ttctcaagta gataaacaac gtgaagattt taaacgtttg ggtgtttctg gtgactggga
    31861 aaatccatat gtgaccttga ctcctgacta tgaagcagct caaattcgtg tatttggtga
    31921 gatggctaat aagggttata tctaccgtgg tgccaagcca gtttactggt catggtcatc
    31981 tgagtcagcc cttgctgaag cagagattga ataccatgac ttggtttcaa cttcccttta
    32041 ctatgccaac aaggtaaaag atggcaaagg agttctagat acagatactt atatcgttgt
    32101 ctggacaacg actccattta ccatcacagc ttctcgtggt ttgacggttg gtgcagatat
    32161 tgattacgtt ttggttcaac ctgttggtga agctcgtaag tttgtcgttg ctgctgaatt
    32221 attgactagc ttgtctgaga aatttggctg ggctgatgtt caagttttgg aaacttaccg
    32281 tggccaagaa ctcaaccaca tcgtaacaga acacccatgg gatacagctg tagaagagtt
    32341 ggtaattctt ggtgaccacg ttacgactga ctctggtaca ggtattgtcc atacagcccc
    32401 tggttttggt gaggacgatt acaatgttgg tattgctaat aatcttgaag tcgcagtgac
    32461 tgttgatgaa cgtggtatca tgatgaagaa tgctggtcct gaatttgaag gtcaattcta
    32521 tgaaaaggta gttccaactg ttattgaaaa acttggtaac ctccttcttg cccaagaaga
    32581 aatctctcac tcatatccat ttgactggcg tactaagaaa ccaatcatct ggcgtgcagt
    32641 tccacaatgg tttgcctcag tttctaaatt ccgtcaagaa atcttggacg aaattgaaaa
    32701 agtgaaattc cactcagaat ggggtaaagt ccgtctttac aatatgatcc gtgaccgtgg
    32761 tgactgggtt atctctcgtc aacgtgcttg gggtgttcca cttcctatct tctacgctga
    32821 agatggtaca gctatcatgg tagctgaaac tattgaacac gtagctcaac tttttgaaga
    32881 acatggttca agcatttggt gggaacgtga tgccaaagac ctcttgccag aaggatttac
    32941 tcatccaggt tcaccaaacg gcgagttcaa aaaagaaact gatatcatgg acgtttggtt
    33001 tgactcaggt tcatcatgga atggagtggt ggtaaaccgt cctgaattga cttacccagc
    33061 cgacctttac ctagaaggtt ctgaccaata ccgtggttgg tttaactcat cacttatcac
    33121 atctgttgcc aaccatggcg tagcacctta caaacaaatc ttgtcacaag gttttgccct
    33181 tgatggtaaa ggtgagaaga tgtctaaatc tcttggaaat accattgctc caagcgatgt
    33241 tgaaaaacaa ttcggtgctg aaatcttgcg tctctgggta acaagtgttg actcaagcaa
    33301 tgacgtgcgt atctctatgg atattttgag ccaagtttct gaaacttacc gtaagattcg
    33361 taacactctt cgtttcttga ttgccaatac atctgacttt aacccagctc aagatacagt
    33421 cgcttacgat gagcttcgtt cagttgataa gtacatgacg atttgcttta accagcttgt
    33481 caagaccatt cgtgatgcct atgcagactt tgaattcttg acgatctaca aggccttggt
    33541 gaactttatc aacgttgact tgtcagcctt ctaccttgat tttgccaaag atgttgttta
    33601 cattgaaggt gccaaatcac tggaacgccg tcaaatgcag actgtcttct atgacattct
    33661 tgtcaaaatc accaaactct tgacaccaat ccttcctcat actgcggaag aaatttggtc
    33721 atatcttgag tttgaaacag aagacttcgt ccaattgtca gaattaccag aggctcaaac
    33781 ttttgctaat caagaagaaa tcttggatac atgggcagcc ttcatggact tccgtggaca
    33841 agctcaaaaa gccttggaag aagctcgtaa tgcaaaagtt atcggtaaat cacttgaagc
    33901 acacttgaca gtttatccaa atgaagtggt gaaaactcta ctcgaagcag taaacagcaa
    33961 tgtagctcaa cttttgatcg tgtctgagtt gaccatcgca gaaggaccag ctccggaagc
    34021 tgcccttagc ttcgaagatg tagccttcac agttgaacgt gctgctggtg aagtatgtga
    34081 ccgttgccgt cgtattgacc caacaacagc agaacgcagc taccaggcag ttatctgtga
    34141 ccactgtgca agcatcgtag aagaaaactt tgcggaagca gtcgcagaag gatttgaaga
    34201 gaaataagat tgaaaagtct aggcaaaatt caatttgaga agaaaagaca actaatttta
    34261 tagtctatta aacgcattgt atcacgtttt tgaacacctg atatgatgcg ttttttattt
    34321 attttaaaaa tttgcgaggt atgacttttt atactcaaca agaatcaaag agaaacttag
    34381 caagctaaca gtagtaagat aaaataggaa tttgatatta gggataagat tggtaaatag
    34441 tgtaatattt ttacaacaat aaatttatat agtta
//
LOCUS       DAVKLX010000018        34232 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKLX010000018 DAVKLX010000000
VERSION     DAVKLX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34232
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.018"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            211..2871
                     /gene="rrgA"
                     /locus_tag="VW325_000941"
     CDS             211..2871
                     /gene="rrgA"
                     /locus_tag="VW325_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus tip adhesin RrgA"
                     /protein_id="HEU9734370.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTDGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQWTVEVEKNGRTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYEQKDKSVPL
                     DVVILLDNSNSMSNIRNKNARRAERAGEATRSLIDKITSDPENRVALVTYASTIFDGT
                     EFTVEKGVADKNGKRLNDSLFWNYDQTSFTTNTKDYSYLKLTNDKNDIVELKNKVPTE
                     AEDHDGNRLMYQFGATFTQKALMKADEILTQQARQNSQKVIFHITDGVPTMSYPINFN
                     HATFAPSYQNQLNAFFSKSPNKDGILLSDFITQATSGEHTIVRGDGQSYQMFTDKTVY
                     EKGAPAAFPVKPEKYSEMKAAGYAVIGDPINGGYIWLNWRESILAYPFNSNTAKITNH
                     GDPTRWYYNGNIAPDGYDVFTVGIGINGDPGTDEATATSFMQSISSKPENYTNVTDTT
                     KILEQLNRYFHTIVTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRL
                     ENGQAVGGPQNDGGLLKNAKVLYDTTEKRIRVTGLYLGTDEKVTLTYNVRLNDEFVSN
                     KFYDTNGRTTLHPKEVEQNTVRDFPIPKIRDVRKYPEITISKEKKLGDIEFIKVNKND
                     KKPLRDAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENS
                     EPAGYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREY
                     PRTGGIGMLPFYLIGCMMMGGVLLYTRKHP"
     gene            3095..5059
                     /locus_tag="VW325_000942"
     CDS             3095..5059
                     /locus_tag="VW325_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEU9734371.1"
                     /translation="MKSINKFLTMLAALLLTASSLFSAATVFAAEQKTKTLTVHKLLM
                     TDQELDAWNSDAITTAGYDGSQNFEQFKQLQGVPQGVTEISGVAFELQSYTGPQGKEQ
                     ENLTNDAVWTAVNKGVTTETGVKFDTEVLQGTYRLVEVRKESTYVGPNGKVLTGMKAV
                     PALITLPLVNQNGVVENAHVYPKNSEDKPTATKTFDTAAGFVDPGEKGLAIGTKVPYI
                     VTTTIPKNSTLATAFWSDEMTEGLDYNGDVVVNYNGQPLDNSHYTLEAGHNGFILKLN
                     EKGLEAINGKDAEATITLKYTATLNALAVADVPEANDVTFHYGNNPGHGNTPKPNKPK
                     NGELTITKTWADAKDAPIAGVEVTFDLVNAQTGEVVKVPGHETGIVLNQTNNWTFTAT
                     GLDNNTEYKFVERTIKGYSADYQTITETGKIAVKNWKDENPEPINPEEPRVKTYGKKF
                     VKVDQKDERLKEAQFVVKNEQGKYLALKSAAQQAVNEKAAAEAKQALDAAIAAYTNAA
                     DKNAAQAIVDAAQKTYNDNYRAARFGYVEVERKEDALVLTSNTDGQFQISGLAAGSYT
                     LEETKAPEGFAKLGDVKFEVGAGSWNQGDFNYLKDVQKNDATKVVNKKITIPQTGGIG
                     TIIFAVAGAVIMGIAVYAYVKNNKDEDQLA"
     gene            5106..6287
                     /gene="rrgC"
                     /locus_tag="VW325_000943"
     CDS             5106..6287
                     /gene="rrgC"
                     /locus_tag="VW325_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HEU9734372.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDNRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKADTVTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDGSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIVVTNLPLGTY
                     RFKEVEPLAGYTVTTMDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEENGHYTPVLQNGKEVVVASGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKTN
                     N"
     gene            6626..7528
                     /gene="srtC-1"
                     /locus_tag="VW325_000944"
     CDS             6626..7528
                     /gene="srtC-1"
                     /locus_tag="VW325_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HEU9734373.1"
                     /translation="MAKTKKQKRNNLLLGVVFFIGMAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPVIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKM
                     FTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYM
                     INTHRLLVRGHRIPYVAEVEEEFIAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKK
                     KQPEKALKALKAARKEVKVEDGQQ"
     gene            7515..8408
                     /gene="srtC-2"
                     /locus_tag="VW325_000945"
     CDS             7515..8408
                     /gene="srtC-2"
                     /locus_tag="VW325_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HEU9734374.1"
                     /translation="MDNSRRSRKKGTKKKKHPLILLLIFLVGFAVAIYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTEQEKKKGVSEYAN
                     MLKVHERIGYVEIPAIDQEIPMYVGTSEEILQKGAGLLEGASLPVGGENTHTVVTAHR
                     GLPTAELFSQLDKMKKGDVFYLHVLDQVLAYQVDQILTVEPNDFEPVLIQHGEDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLAALVMILVLSYGV
                     YRHRRIVKGLEKQLEEHHVKG"
     gene            8395..9246
                     /locus_tag="VW325_000946"
     CDS             8395..9246
                     /locus_tag="VW325_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEU9734375.1"
                     /translation="MSKAKLQKLLGYLLMLVALVIPVYCFGQMVLQSLGQVKGHEIFS
                     ESVTADSYQEQLQRSLDYNQRLDSQNRIVDPFLAEGYEVNYQVSDDPDAVYGYLSIPS
                     LEIMEPVYLGADYHHLAMGLAHVDGTPLPVEGKGIRSVIAGHRAEPSHVFFRHLDQLK
                     VGDALYYDNGQEIVEYQMMDTEIILPSEWEKLESVSSKNIMTLITCDPIPTFNKRLLV
                     NFERVAVYQKSDPQTAAVARVAFTKEGQSVSRVATSQWLYRGLVVLAFLGILFVLWKL
                     ARLLRGK"
     gene            complement(9319..10595)
                     /locus_tag="VW325_000947"
                     /pseudo
     CDS             complement(9319..10595)
                     /locus_tag="VW325_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(10710..10859)
                     /locus_tag="VW325_000948"
     CDS             complement(10710..10859)
                     /locus_tag="VW325_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734376.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVD
                     AFMAL"
     gene            complement(10852..11136)
                     /locus_tag="VW325_000949"
     CDS             complement(10852..11136)
                     /locus_tag="VW325_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734377.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            complement(12570..13793)
                     /locus_tag="VW325_000950"
     CDS             complement(12570..13793)
                     /locus_tag="VW325_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9734378.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            13961..15283
                     /locus_tag="VW325_000951"
     CDS             13961..15283
                     /locus_tag="VW325_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9734379.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDSVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            15285..17171
                     /locus_tag="VW325_000952"
     CDS             15285..17171
                     /locus_tag="VW325_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU9734380.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEEKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRTLNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            17339..17683
                     /locus_tag="VW325_000953"
     CDS             17339..17683
                     /locus_tag="VW325_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9734381.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            17693..19105
                     /gene="lacG"
                     /locus_tag="VW325_000954"
     CDS             17693..19105
                     /gene="lacG"
                     /locus_tag="VW325_000954"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9734382.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            19175..20854
                     /locus_tag="VW325_000955"
     CDS             19175..20854
                     /locus_tag="VW325_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9734383.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSTFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
     gene            complement(20980..22419)
                     /locus_tag="VW325_000956"
     CDS             complement(20980..22419)
                     /locus_tag="VW325_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9734384.1"
                     /translation="MNKSMIHYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(22423..23772)
                     /gene="trkA"
                     /locus_tag="VW325_000957"
     CDS             complement(22423..23772)
                     /gene="trkA"
                     /locus_tag="VW325_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9734385.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKEVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            23960..24808
                     /locus_tag="VW325_000958"
     CDS             23960..24808
                     /locus_tag="VW325_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9734386.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            24823..25371
                     /locus_tag="VW325_000959"
     CDS             24823..25371
                     /locus_tag="VW325_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9734387.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            25378..25626
                     /locus_tag="VW325_000960"
     CDS             25378..25626
                     /locus_tag="VW325_000960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734388.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVR"
     gene            25737..27419
                     /locus_tag="VW325_000961"
     CDS             25737..27419
                     /locus_tag="VW325_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734389.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKKEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            27404..28234
                     /locus_tag="VW325_000962"
     CDS             27404..28234
                     /locus_tag="VW325_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9734390.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGAYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            28427..28930
                     /locus_tag="VW325_000963"
     CDS             28427..28930
                     /locus_tag="VW325_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9734391.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      28984..29218
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            29383..29946
                     /locus_tag="VW325_000964"
     CDS             29383..29946
                     /locus_tag="VW325_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9734392.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            29960..30586
                     /locus_tag="VW325_000965"
     CDS             29960..30586
                     /locus_tag="VW325_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9734393.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            30613..31029
                     /locus_tag="VW325_000966"
     CDS             30613..31029
                     /locus_tag="VW325_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734394.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFFGLKSRRRENGFQLGILHYLRIISPL"
     gene            30927..31238
                     /locus_tag="VW325_000967"
                     /pseudo
     CDS             30927..31238
                     /locus_tag="VW325_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31366..31953
                     /locus_tag="VW325_000968"
     CDS             31366..31953
                     /locus_tag="VW325_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9734395.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            32343..33950
                     /locus_tag="VW325_000969"
     CDS             32343..33950
                     /locus_tag="VW325_000969"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9734396.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT        10450 a         5709 c         7588 g        10485 t
ORIGIN      
        1 tatacaagtg tgtcattgcc aggttgagaa gatagctata acgcactttt atacgctttt
       61 gctacgtttg ttagtgaacg gattaactca gtgagataaa ttttatcaga acataagtaa
      121 tccgtttctt cgtgtataca gattgaaagt acctatgaat catagaagga ttaacttgtt
      181 ctatgaataa tgcttaacag ggagacacac atgaaaaaag taagaaagat atttcagaag
      241 gcagttgcag gactgtgctg tatatctcag ttgacagctt tttcttcgat agttgcttta
      301 gcagaaacgc ctgaaaccag tccagcgata ggaaaagtag tgattaagga gacaggcgaa
      361 ggaggagcgc ttctaggaga tgccgtcttt gagttgaaaa acaatacgga tggcacaact
      421 gtttcgcaaa ggacagaggc gcaaacagga gaagcgatat tttcaaacat aaaacctggg
      481 acatacacct tgacagaagc ccaacctcca gttggttata aaccctctac taaacaatgg
      541 actgttgaag ttgagaagaa tggtcggacg actgtccaag gtgaacaggt agaaaatcga
      601 gaagaggctc tatctgacca gtatccacaa acagggactt atccagatgt tcaaacacct
      661 tatcagatta ttaaggtaga tggttcggaa aaaaacggac agcacaaggc gttgaatccg
      721 aatccatatg aacgtgtgat tccagaaggt acactttcaa agagaattta tcaagtgaat
      781 aatttggatg ataaccaata tggaatcgaa ttgacggtta gtgggaaaac agtgtatgaa
      841 caaaaagata agtctgtgcc gctggatgtc gttatcttgc tcgataactc aaatagtatg
      901 agtaacattc gaaacaagaa tgctcgacgt gcggaaagag ctggtgaggc gacacgttct
      961 cttattgata aaattacatc tgatccagaa aatagggtag cgcttgtgac ttatgcttcc
     1021 actatctttg atgggaccga gtttacagta gaaaaagggg tagcagataa aaacggaaaa
     1081 cgattgaatg attctctttt ttggaattat gatcagacga gttttacaac caataccaaa
     1141 gattatagtt atttaaagct gactaatgat aagaatgaca ttgtagaatt aaaaaataag
     1201 gtacctaccg aggcagaaga ccatgatgga aatagattga tgtaccaatt cggtgccact
     1261 tttactcaga aagctttgat gaaggcagat gagattttga cacaacaagc gagacaaaat
     1321 agtcaaaaag tcattttcca tattacggat ggtgtcccaa ctatgtcgta tccgattaat
     1381 tttaatcatg ctacgtttgc tccatcatat caaaatcaac taaatgcatt ttttagtaaa
     1441 tctcctaata aagatggaat actattaagt gattttatta cgcaagcaac tagtggagaa
     1501 catacaattg tacgcggaga tgggcaaagt taccagatgt ttacagataa gacagtttat
     1561 gaaaaaggtg ctcctgcagc tttcccagtt aaacctgaaa aatattctga aatgaaggcg
     1621 gctggttatg cagttatagg cgatccaatt aatggtggat atatttggct taattggaga
     1681 gagagtattc tggcttatcc gtttaattct aatactgcta aaattaccaa tcatggtgac
     1741 cctacaagat ggtactataa cgggaatatt gctcctgatg ggtatgatgt ctttacggta
     1801 ggtattggta ttaacggaga tcctggtacg gatgaagcaa cggctactag ttttatgcaa
     1861 agtatttcta gtaaacctga aaactatacc aatgttactg acacgacaaa aatattggaa
     1921 cagttgaatc gttatttcca caccatcgta actgaaaaga aatcaattga gaatggtacg
     1981 attacagatc cgatgggtga gttaattgat ttgcaattgg gcacagatgg aagatttgat
     2041 ccagcagatt acactttaac tgcaaacgat ggtagtcgct tggagaatgg acaagctgta
     2101 ggtggtccac aaaatgatgg tggtttgtta aaaaatgcaa aagtgctcta tgatacgact
     2161 gagaaaagga ttcgtgtaac aggtctgtac cttggaacgg atgaaaaagt tacgttgacc
     2221 tacaatgttc gtttgaatga tgagtttgta agcaataaat tttatgatac caatggtcga
     2281 acaaccttac atcctaagga agtagaacag aacacagtgc gcgacttccc gattcctaag
     2341 attcgtgatg tgcggaagta tccagaaatc acaatttcaa aagagaaaaa acttggtgac
     2401 attgagttta ttaaggtcaa taaaaatgat aaaaaaccac tgagagatgc ggtctttagt
     2461 cttcaaaaac aacatccgga ttatccagat atttatggag ctattgatca aaatggcact
     2521 tatcaaaatg tgagaacagg tgaagatggt aagttgacct ttaaaaatct gtcagatggg
     2581 aaatatcgat tatttgaaaa ttctgaacca gctggttata aacccgttca aaataagcct
     2641 atcgttgcct tccaaatagt aaatggagaa gtcagagatg tgacttcaat cgttccacaa
     2701 gatataccag cgggttacga gtttacgaat gataagcact atattaccaa tgaacctatt
     2761 cctccaaaga gagaatatcc tcgaactggt ggtatcggaa tgttgccatt ctatctgata
     2821 ggttgcatga tgatgggagg agttctatta tacacacgga aacatccgta aagtgtagaa
     2881 atgataatat ctatgttctg aacgatactt ttaagaagta gcactcaaga agagatttaa
     2941 gtttacttgg tgaaacctgt tttattcgta agtaaactat cattgaaagg ggagatgttt
     3001 tcgaaaactt gcacagaaaa aggattatta ttgtcatgtg taattcatta cattgctcac
     3061 agttgatttt aagagatatg aataaggaga aatcatgaaa tcaatcaaca aatttttaac
     3121 aatgcttgct gccttattac tgacagcgag tagcctgttt tcagctgcaa cagtttttgc
     3181 ggcggaacaa aaaactaaga cacttacagt tcataaatta ttgatgacag atcaagagct
     3241 tgacgcttgg aattctgatg cgattactac tgcaggttat gacggttcgc aaaattttga
     3301 acagttcaaa caacttcaag gtgttccaca aggagtaacc gaaatctctg gtgttgcatt
     3361 cgagttacag agttatacgg gtcctcaagg aaaagaacaa gaaaatttaa cgaatgatgc
     3421 ggtttggact gcggttaata aaggtgtgac gactgaaacg ggtgttaaat ttgatactga
     3481 agttttacaa gggacatatc gtcttgtcga agtacgtaaa gaatcgactt atgtcggtcc
     3541 aaatggtaaa gttttaacag gtatgaaagc tgttcctgct ttaattactc tgccgcttgt
     3601 aaaccaaaat ggtgttgtag aaaatgcaca tgtctatcca aagaattctg aagacaaacc
     3661 tacagcaacg aaaacatttg atactgcagc aggtttcgta gatccaggtg aaaaaggttt
     3721 agcaattggc actaaggtac cgtatattgt tacaacaact attccgaaaa actcaactct
     3781 tgcaacagct ttctggtcag atgaaatgac agaaggtcta gattataatg gtgatgtagt
     3841 tgttaattat aatggtcaac cgcttgataa ttctcattac acattagaag caggtcataa
     3901 tggctttatc ttgaagttaa atgaaaaagg tctggaagca atcaacggta aagatgcaga
     3961 agcaacaatt acgttgaagt atactgcaac tttaaatgct cttgctgttg ctgatgtgcc
     4021 agaagcgaat gatgtaacat tccattatgg aaacaaccca ggtcatggta acactccaaa
     4081 accaaacaaa cctaaaaacg gtgaacttac aattactaaa acatgggctg atgctaaaga
     4141 tgctcctata gcaggtgtag aagtaacttt tgatttggta aatgctcaga caggtgaggt
     4201 cgttaaagta cctggacatg aaacaggtat tgtattgaat caaacaaata attggacatt
     4261 tactgctaca ggtcttgata ataatacaga atataaattt gttgaacgga caattaaggg
     4321 atattctgca gattaccaaa caattactga aacaggaaaa attgctgtta aaaactggaa
     4381 agatgaaaat ccagaaccaa taaatcctga agagccacgt gtaaaaacat atggtaaaaa
     4441 attcgttaag gttgaccaaa aagacgaacg cttaaaagaa gcacaattcg ttgtgaagaa
     4501 tgagcaaggg aaatatcttg cactcaaatc tgcagcacaa caagctgtaa atgagaaagc
     4561 tgccgcagaa gcgaaacaag cgctagatgc agcaatagca gcctatacaa atgctgcaga
     4621 taaaaatgca gcacaagcta tagtagatgc tgcgcaaaaa acatataatg acaattacag
     4681 agcagctaga tttggctatg tagaagtaga gagaaaagaa gatgcgttag ttcttacttc
     4741 taacactgat ggtcaattcc aaatttcagg tcttgctgct ggaagctaca cgttggaaga
     4801 aacaaaagct ccagaaggct ttgcaaaact tggagatgtg aagtttgagg ttggagcagg
     4861 ttcttggaat caaggtgatt tcaattattt aaaagatgtt cagaagaacg acgctacaaa
     4921 agtagtcaac aaaaaaatca cgatccctca aacgggtggt attggtacaa ttatctttgc
     4981 tgtagcgggg gctgtgatta tgggtattgc agtgtacgca tatgttaaaa acaacaaaga
     5041 tgaggatcaa cttgcttaag taagagagaa aggagccatt gatgacaatg cagaaaatgc
     5101 agaaaatgat tagtcgtatc ttctttgtta tggctctgtg tttttctctt gtatggggtg
     5161 cacatgcagt ccaagcgcaa gaagatcaca cgttggtctt gcaattggag aactatcagg
     5221 aggtggttag tcaattgcca tctcgtgatg gtcatcggtt gcaggtatgg aagttggatg
     5281 attcgtattc ctatgataat cgggtgcaaa ttgtgagaga cttgcattcg tgggatgaga
     5341 ataaactttc ttctttcaaa aagacttcgt ttgagatgac cttccttgag aatcagattg
     5401 aagtatctca tattccaaat ggtctttact atgttcgctc tattatccag acggatgcgg
     5461 tttcttatcc agctgaattt ctttttgaaa tgacagatca gacggtagag cctttggtca
     5521 ttgtagcgaa aaaagcagat acggtgacaa caaaggtgaa gctgataaag gtggatcaag
     5581 accacaatcg cttggagggt gtcggcttta aattggtatc agtagcaaga gatggttctg
     5641 aaaaagaggt tcccttgatt ggagaatacc gttacagttc ttctggtcaa gtaggtagaa
     5701 ctctctatac tgataaaaat ggagagattg ttgtgacaaa tcttcctctt gggacctatc
     5761 gtttcaagga ggtggagcca ctggcaggct atactgttac gacgatggat acggatgtcc
     5821 agttggtaga tcatcagctg gtgacgatta cggttgtcaa tcagaaatta ccacgtggca
     5881 atgttgactt tatgaaggtg gatggtagga ccaatacctc tcttcaaggg gcaatgttca
     5941 aagtcatgaa agaagaaaac ggacactata ctcctgttct tcaaaatggt aaggaagtag
     6001 ttgtggcatc agggaaagat ggtcgtttcc gagtggaagg tctagagtat gggacatatt
     6061 atttatggga gctccaagct ccaactggtt atgttcaatt aacatcgcct gtttccttta
     6121 caatcgggaa agatactcgt aaggaactgg taacagtggt taaaaataac aagcgaccac
     6181 ggattgatgt gccagataca ggggaagaaa ccttgtatat cttgatgctt gttgccattt
     6241 tgttgtttgg tagtggctat tatcttacga aaaaaacaaa taactgatat tcaatgtaca
     6301 tcattatgaa aaagatagca ggctgaaggg aagaccagag tactctgagg tgatgttaat
     6361 caggaatcat ggtgatttgg catgaatcac aataacggat atgaggctgg gcagattgtg
     6421 ccagcctcat tgtgggttat tgtttgtaaa acgataggac tggtctggta atcattttag
     6481 gaatggacag gactgggatt ctgatttaaa atggatggtg aatcagaaag aaatgagatt
     6541 ttctcgtttc tcttagcaga taggattgtc tgttaggaaa agcgataaaa tgatgagttt
     6601 gaagttaaag gaatgctgat aaaaaatggc aaaaacaaaa aagcaaaaac gaaacaatct
     6661 cctattagga gtggtatttt tcattggaat ggcggtaatg gcgtatccgc tggtgtctcg
     6721 cttgtattat cgagtggaat caaatcaaca aattgctgac tttgataagg aaaaagcaac
     6781 gttggatgag gctgacattg atgaacgaat gaaattggca caagccttca atgactcttt
     6841 gaataatgta gtgagtggcg atccttggtc ggaagaaatg aagaaaaaag ggcgagcaga
     6901 gtatgcacgt atgttagaaa tccatgagcg gatggggcat gtggaaatcc ccgttattga
     6961 cgtggatttg ccggtttatg ctggtactgc tgaagaggta ttgcagcaag gggctgggca
     7021 tctagaggga acttctctgc cgatcggagg caattcgacc catgcggtga ttacggcaca
     7081 tacaggtttg ccaacagcta agatgtttac ggatttgacc aaacttaaag ttggggataa
     7141 gttttatgtg cacaatatca aggaagtgat ggcctatcaa gtggatcaag taaaggtgat
     7201 tgagccgacg aactttgatg atttattgat tgtaccaggt catgattatg tgaccttgct
     7261 gacttgtacg ccatacatga tcaataccca tcgtctattg gttcgggggc atcggatacc
     7321 gtacgtagca gaggttgagg aagaatttat tgcagcaaac aaactcagtc atctctatcg
     7381 ctacctgttt tatgtggcag ttggtttgat tgtgattctt ttatggatta ttcgacgctt
     7441 gcgcaagaag aaaaaacaac cggaaaaggc tttgaaggcg ctgaaagcag caaggaagga
     7501 agtgaaggtg gaggatggac aacagtagac gttcacgaaa aaaaggcaca aaaaagaaga
     7561 aacatccgct gatccttctt ctgattttct tagtaggatt cgccgttgcg atatatccat
     7621 tggtgtctcg ttattattat cgtattgagt caaacgaggt tattaaagag tttgatgaga
     7681 cggtttccca gatggataag gcagaacttg aggagcgttg gcgcttggct caagccttca
     7741 atgcgacctt gaaaccatct gaaattcttg atccttttac agagcaagag aaaaagaaag
     7801 gcgtctcaga atatgccaat atgctaaagg tccatgagcg gattggctat gtggaaattc
     7861 ctgcgattga tcaggaaatt ccgatgtatg tcggaacgag tgaggaaatt cttcagaagg
     7921 gcgcaggatt gctagaggga gcttcgttac cggttggtgg tgaaaatacc cacacagttg
     7981 tcactgctca tagaggatta ccgacggcag aactgtttag tcaattggat aagatgaaaa
     8041 aaggggatgt cttttatctt cacgttttag accaggtgtt ggcctaccaa gtggatcaga
     8101 ttttgacggt tgagccaaat gactttgagc ctgtcttgat tcaacatggg gaagattatg
     8161 cgaccttgtt gacctgtaca ccgtatatga ttaacagtca tcgtctgttg gtacgtggga
     8221 agcggattcc gtatacggca ccaattgcag agcgaaatcg agcggtgaga gagcgtgggc
     8281 aattctggtt gtggttattg ctagcggcgt tggttatgat tctggtattg agttacgggg
     8341 tgtatcgtca tcgtcgcatt gtcaaagggc tagaaaaaca attggaggag catcatgtca
     8401 aaggctaagc tacagaaatt actagggtat ttgctgatgc tggtagcatt ggtgattcct
     8461 gtttattgtt ttgggcagat ggtgttacag tctttaggac aagtaaaagg tcatgagata
     8521 ttttcagaat ctgtgacggc cgacagttac caagagcaat tgcaacggtc gcttgattac
     8581 aatcaacgct tggattcgca aaatcgtatt gtagatcctt ttttggcgga agggtatgag
     8641 gtaaattacc aagtgtctga cgatcctgat gcagtctacg gctatttgtc gattccgagt
     8701 ttggaaatca tggagccagt ttatctagga gcggattacc atcatttagc aatggggttg
     8761 gcccatgtgg atgggacgcc tcttcctgtt gagggaaaag ggattcgttc agtgattgct
     8821 gggcaccgtg cagaaccaag ccatgtcttt ttccgccatt tggatcagct aaaagttgga
     8881 gatgctcttt attatgataa tggccaggaa attgtagaat atcagatgat ggacacagag
     8941 attattttac cgtcggaatg ggaaaaatta gaatcggtta gctctaaaaa tatcatgacc
     9001 ttgataacct gcgatccgat tcctaccttt aataaacgct tattagtgaa ttttgaacga
     9061 gtcgctgttt atcaaaaatc agatccacaa acagctgcag ttgcgagggt tgcttttacg
     9121 aaagaaggac aatctgtatc gcgtgttgca acctctcaat ggttgtaccg tgggctagtg
     9181 gtactggcat ttctgggaat cctgtttgtt ttgtggaagc tagcacgttt actacgaggg
     9241 aaataaaaag aaatgaaagg aaagctaagg ctgttccttt ttccggctct ttgtcaactg
     9301 tagtgggttg aaaaaaagct aagctcgaga aaggacaaat tttgtccttt cttttttgat
     9361 attcagagcg ataaaaatcc gttttttgaa gttttcaaag tttcgaaaac caaaggcatt
     9421 gcgcttgata agtttgatga gattattggt cgcttccagt ttggcattag aatagtgtag
     9481 ttgaagggcg ttgataatct tttctttatc tttgaggaag gttttaaaga cagtctgaaa
     9541 aataggatga acctgcttaa gattgtcctc aataagtccg aaaaatttct ctggttcctt
     9601 attctggaag tgaaacagca agagctgata gagctgatag tggtgtttca agtcttgtga
     9661 atggctcaaa agcttgtcta aaatctcttt attggttaag tgcatacgaa aagtaggacg
     9721 ataaaatcgc ttatcactca gtctacggct atcctgttga atgagtttcc agtagcgctt
     9781 gatagccttg tattcatggg attttcgatg aaactgattc atgatttgga catgcacacg
     9841 actcatagca cggctaagat gttgtacaat gtgaaagcga tcaagaacga ttttagcaca
     9901 cgggaaaagt tgtctagcta agtcatagta aggactaaac atatccatag taatgatttt
     9961 gacgcgacat cggacggctc tatcatattt aagaaagtga tctcggatga cagcttgtgt
    10021 tctaccttca agaacagtga tgatatcgag cttttcaaaa tcttgcgcaa taaagctcat
    10081 ctccatctcc cgattgaaac agtcactccc cggactgttt caacgtccca ggacataatc
    10141 tcaggaagac gcgaaaaatc atgctcaaag tgagaatcat tgagcttgcg aatgacagtt
    10201 gaagttgaaa tggccagctg atgagcaata tcggtcatag aaatcttctc aatcaacttt
    10261 tgcgcaattt tttggttgat aatacgagga atttgatgat tcttcttgac gatagaagtt
    10321 tcagcgacca tcatttttga acagtgatag cacttgaaac ggcgttttct aaggagaatt
    10381 ctagaaggca taccagttgt ttcgaggtaa gggatcttag acggtttttg aaagtcatat
    10441 ttcttcaatt ggtttccgca ctcagggcaa gatggggcgt cgtagtccag tttggcgatg
    10501 atttccttgt gtgtatcctt attgatgatg tctaaaatct ggatattagg gtctttaatg
    10561 tctagtaatt ttgtgataaa atgtaattgt tccatatgat tctttctaat gagttgtttt
    10621 gtcgcttttc attataggtc atatgggact ttttttctac acaaaaatag gctccataat
    10681 atccataggg gatttaccca ctacaaatat tatagagcca taaaagcatc aacatcttgt
    10741 gtggcacgta aaccatgata ttctaagaca taaccaccga cacagataat tgttaaagtc
    10801 agattttcct gaattaattc cttatttagg gcttggaaga ccggtttcat tttatccatg
    10861 tttgtatttc tccatcaatt tttgaacatt ggttaactta gcaggtttat ttaatgaaat
    10921 cacatattta tgagttggag tatgctcttg aaaggcatac ttcaagctaa ttttttgaaa
    10981 acgggttagt tctttggagt ctagcaactt ttctacatct tggtttgtta aataagaata
    11041 gtcttcaaaa ataacttttc gcaaatagcg aaaccaatgg cttgcgttga attgtgattg
    11101 tttggatgcc tgtatcatat aatcgtacca atccattgtc ttcctcctat aaaatttagt
    11161 ttatattatt gtagtttata ttcaaaaatg tagtttttct taattagctc gtatctatag
    11221 gatgatactg aaaatcttct tgttgcttta aatcacaatc gaaaaattct gtttttcatc
    11281 ttagaaaaga atgtttagaa atgtaaaatg aacctttttt cttatccgaa aaccttacta
    11341 aagtatataa tttaggaagt caatcctgta tgataatgtt tgtactatcg tcgagttttt
    11401 gcttgattgc ctaacgagat aaattttaga agaattttcg gtgtgatttg acaagcttct
    11461 gactggaagg atagttacac tagtattgtt tattaaaatg agcagataga gaaaatttgg
    11521 aatctatttg cttattatca atcgtttttt tagcttattt gtcttagatt ggtatagaac
    11581 tgagctatgg attaatactc ttgtatctta tcctgttcct aaatatgttg gtagaaaaat
    11641 gaagcagttg ttacgagtgt ctgtattgga aaaaattttg tctatagaat tctttaaatg
    11701 atatgaaggc taggaatgcc aaagcactga catatttcgt ttgaatctca tccgctctct
    11761 ttacttgtat ttgtagaaat gctatcagta atatattcgc gaacactatg atttacaaca
    11821 gtgtggtcgt acatctttgt aactttatta tacaacaacc atttacaggt gtaaagttga
    11881 gcagtgtctt tagtaaattt agggatttgt tttgtcatag ttttgatacc tgtattcgtt
    11941 gtttcatgag catttgacgt ttcttttcat gtaggcattt caacaattag aatgatattt
    12001 ttccgaggat gaacttatat attgtgactt tttagagtta tgatgcctct tgatttgact
    12061 atcgctatca atttcatcca cttttacagt ttcatggttt tatgaaccga aacatctagg
    12121 tacaggtttt agcatcaatt gattgttttt gagccatatg aagttgaaac gtggagatgc
    12181 ttttcttgtg ataacgccct attcaaagag gaataaccgt ttttggagag agttcagtac
    12241 tatgtggcgc taaatactat atgtaaaaca tatattaaac aagatttaac taaggagaat
    12301 tgtaaaaaca aaaatgagaa tttcatataa agtttattct aggagtatgt atcttgaata
    12361 aaaattgtta tttaagttct gggggttatg atgaatgttt ttataataat aaaatgcagc
    12421 tagaagaaaa gttattttaa tataaatcac tcaacatagc gagaaggcgg tttcctatat
    12481 ggaagtcgtc ttgttttcat attgtctaaa tacgagatat ctactacagg ttatagttga
    12541 tttaaacagg tctatagatt tctaatatat taaggtgaaa taatatcttg taatacattg
    12601 ctatgttcta tgaaaaaagc gacgatagct aatgcacaag ctcctatact tccacgatgt
    12661 ttgttgtaag gtaaaatttc aatttctata ttacgagtaa agggaacgaa gtggagctgg
    12721 tcttggattt tatccctgac ttcatgagtg aaaggttgat aattaagcaa ttgactgttg
    12781 atatagattt tatcagcagg attgatgagg aggagatttg caatagaagt ggctaataaa
    12841 ttgactcctt tatcaatttg ttgacgtaaa gcggagtcgc ctaaattata agccgttaaa
    12901 agggtgtcta aatgaatgtc tttttcagtc tttacaaggc tttttagtac agttagttgg
    12961 gaatttttaa ataataattg ggcgtgtttg attaaccaag catccgaaat atatgtttgt
    13021 aaacaacctt tttttccgca ttcacaatat tgcccattca attcgacaat ggtgtgtcca
    13081 atttctccga tataaggatt tttagaggct cctatttttt cttttgtgaa aaaggaagtg
    13141 taaataccta atccagcgtg taggaaaata aagttatcgg gggtattgtg tggattaaaa
    13201 aggtattgtc ctatagccat acaatcgata ttatttttta caataaaagg aaaattgaat
    13261 cttttaatta catttaataa attaaaagat tcccatatgg ggttgtttgt aatgatactt
    13321 ccggagtctt tgttataatg tccaggtatt gataagccaa atccaagtaa ttttgtttcc
    13381 aaagggacgt actctatagc tgtttttatc atattggtaa tggtggaatt gatttctgaa
    13441 ataggataat cttcattcaa aatttctgtc tcgaaaagag ttttcccttg taagtcacat
    13501 agactgagaa agatacctag gacgtttatt tcaatacctc caacaacata cggaatatta
    13561 tctgtgatag aaatagtttt tctacgtctt ccagatccgg aaaactcgct aaattcatca
    13621 ccagtttcgt atatgaggga ctgttcgctt agctctttta tgatgttagt cgttgttgct
    13681 ggagtaagtc ctgtttcatt aacaatatct gtacgggatg ttgctggatt tcgatataga
    13741 aactccagta tagtggctct attttgtaaa aaggctgatt gttttgtaat catttgtttc
    13801 ctcctttggt ttattttata ttaaacataa gaaaaaatca attaaaatta ttattctata
    13861 caaagaaaaa aatattgtaa aaacaacaaa aaatatattg acaacgcttt ctgttagtgg
    13921 tatatttaat ttataaaaaa taaaaaaagg agttccggtg atgaacaata ttttagcgtt
    13981 tttagaaaca aaagtcgctc cgtttggtga aaaagttggc aaccaacggc atttgaaagc
    14041 tattcgtgaa ggatttatga tggcaatgcc tttgatttta gtcggatctt tatttcttat
    14101 tctaatcagt tggcctcaag aggattttac aaattggctg aatagtgttg gattgctaag
    14161 tatcttgaca actatgaatc agtcaacagt agcgattatc tccttggtcg cttgtttcgg
    14221 tattgcctac aggttgtcgg aaggatatgg tacagatggt ccgtcggcag ggatcatagc
    14281 cttatccagt tttgtattga tggcacctcg tttttcgagt atggtttatg ataaaaatgg
    14341 ggagcaggtc aagcagttat ttggcggcgc aataccattt tctagcctga atgcatcttc
    14401 tttgtttatg gcgattacta ttggattggt tacagcagag atttatcgta tgtttatcca
    14461 gcgcggaatt acgataaaaa tgccaagtgg tgtcccagat gtggtaagta aatcattttc
    14521 agctctttta cctggcttta ctacttttgt tttgtgggct ttggtcttaa aaggtcttga
    14581 agcggcagga gttgcaggag gtctcaacgg actcctaggt gcaattgttg gaacaccgct
    14641 taagttaatt gcaggaacgc ttccaggtat gattctatgt gttattgtaa actcattctt
    14701 ttggttctgt ggagttaatg ggggacaagt tttaaatgct tttgtagact cagtttggtt
    14761 acaatttact acagaaaacc aagaagctgt ggctgcagga caaacactcc aacacattat
    14821 tacattaccg tttaaagatt tatttgtatt tattggtggc ggtggagcga ctattggtct
    14881 tgcgatttgt ctcttcctat ttagtaagag tcgtgcgaat aaaacattag gtaagctagc
    14941 tattataccg tctattttta atatcaatac agctattcta tttacgtttc caacagtttt
    15001 aaatccgatt atgctgattc cgtttattgc tactcctaca atcaatgcct tgattaccta
    15061 tgtatcaatg gctgtaggat tagtacccta tacaacaggt gtaatccttc cgtggacaat
    15121 gccaccgatt ataggaggct tccttgcaac aggggctagt tggcgaggag ctctattaca
    15181 agttgttttg attttggttt ctgtagcaat ttattatcca ttcttcaaaa ttgcagataa
    15241 acgcaatctt gaaaaagaaa aagctactgt tggagggaaa taagatggtt atcagagtat
    15301 ttgatcaaca gaaaaatact tattctagct ttgccttaga ggaattaagt tactatatga
    15361 atcgggtctt taagactaac atagagcttg tcgaggagaa ggaagcggat atttttgtag
    15421 gattagtcaa taaagaggac agaaaagacc atgttcttat ctcattagac aagggtaagg
    15481 ggagaattga gtctaataca attgtaggtt tacttattgg aatttaccga atgtttcatg
    15541 aatttggggt tgtgtatact agaccagggc gcagacatga ctttgttcca gagttacgat
    15601 ttgaagattt tttagataaa cagctatcta tagatgaaac agccagttac tatcataggg
    15661 gagtatgtat agagggagcg gattcatttg aaaatatact agatttcatt gattggctac
    15721 ctaagattgg gatgaacagt tttttcatcc agtttgaaaa tccttactct tttttgaaac
    15781 gttggtatga acatgagttt aatccatacc taaataaaga aaaattctca aatgaattag
    15841 tacaagaatt gagtgatagg ttggataaag aattgcaaaa aagaggtctt attcatcatc
    15901 gtgttggtca tggatggaca ggtgaggttt taggttactc ttcaaaattt ggctgggaat
    15961 caggtcttag tatttcagag gaggagaaac cgtatgtcgc tgaaataaac gggaaacgag
    16021 aattatttaa tacggctccg attttaacca gtctggattt ttcaaatcca gatgtggcgg
    16081 ataagatggt agaaattatc aaggattatg ccaagaaaag acctgatgtt aactacttac
    16141 atgtatggtt gtcggatgct cgtaataata tttgtgaatg cgaaaactgt agacaagaat
    16201 tggtttcgga tcagtatatt cgtactctca atcaattgga tagggcttta acgagtgagg
    16261 gattagatac aaagatttgt tttctgcttt accatgagtt gctatgggcg cctcagaaag
    16321 aaaaattaga taatcctgaa cgctttacca tgatgtttgc accgattaca agaacatttg
    16381 aaatgagtta tgcagatgta gattttgaca attccatacc tacgcctaaa ccttatctgc
    16441 gtaataaaat tatacttccg aattctcttg aggaaaattt atcttatctt tttgagtggc
    16501 aaaaaacatt taaaggagat agtttcgtat atgactatcc tttagggcgt gctcattatg
    16561 gcgatttagg ctatatgaaa attagtcaaa ccatttacag agatgtatct tatctttcta
    16621 acctacattt gaacggttac atttcgtgtc aagaattacg tgccggattc cctcataatt
    16681 ttcctaatta tgttatgggg gaaatgctct ggaagaagac aagaagttat gaagaattga
    16741 ttgaagaata cttttctgct ttgtatgggg aaaattggca gtctgttgtt gaatatttag
    16801 aaaaattatc cagttattcc tcatgtgatt attttaatgc aattggcagt cgtcaaaatg
    16861 atgttttagc gaatcattat tatatagctt acaatctagc tgataatttt ttgccaatta
    16921 ttgaggaaaa tatttctaag ttattaaata gtcaaaagga tgaatggaaa cagctcagtt
    16981 atcatcgtga atatgttgtt aagatggcga aggctttata tcttcaagca accggaaaaa
    17041 caaggcaagc tcaagatgaa tggagaaatg tgttgaatta tatccgtggg cacgaactgc
    17101 tattccaatc taatttggat gtttatcgtg taattgaagt agcaaaaaat tacgctggtt
    17161 tccacttata aatcataagt atagaaaatg aaccaaggta ttcagagaag attgatccta
    17221 aatattatga aatttaagga tttttaagat atttagggtc gactttctat ttatatcgta
    17281 gcgaagtcat tttaataatg atgtgtaaaa gatggatcaa gattgaggag gaagaaagat
    17341 gaaatcaaaa gaagaaataa atatgcttgg ttttacaatt gtcgcttacg caggagatgc
    17401 aaggtcagat ttgatggatg ctttggcgtt tgcgagagat ggatattttg aacaggcaag
    17461 agaattggtt gagtctgcaa acgactcaat agtgtctgcc catcgagaac agactaattt
    17521 attagcggag gaggcatctg gagataattt tgaagtgagt tttatcatga ttcatggtca
    17581 agatactttg atgacaacga tgctattgta tgatcaggtc aagtttttta ttgatgaata
    17641 tgaacgaatt cgaaagattg aagaacatat tggtttgcaa tgaggattag tcatggaaaa
    17701 tttacaggtt aaagccttac cgaaggagtt tttattagga gctgctaccg ctgcttatca
    17761 agtagagggt gcaactaggg tagatggcaa aggaacaaat atgtgggatg tttatttgca
    17821 agaaaacagt ccgttcttac cagatccagc tagtgatttt tattatcgtt acgaagagga
    17881 tatagctttg gcggcagaac atggtttgca ggctttgcgt ttatctattt cttgggttcg
    17941 tatatttcct gatatagatg gggagcctaa tatattagct gttcgttatt accatagagt
    18001 ctttcagtct tgcttaaaac ataatgtgat tccgtttgtt tctttacatc attttgattc
    18061 gcctcagaaa atgttagaaa caggggattg gttgaataga gagaatattg atcgtttcat
    18121 acgatatgct cgcttttgtt tccaagaatt tacagaagtc aagcattggt ttacaatcaa
    18181 tgaactgatg tctcttgctg caggtcaata tataggaggt cagtttcctc caaatcatca
    18241 ttttcaatta tctgaagcaa ttcaagcgaa tcataatatg ttgttggcgc atgctcttgc
    18301 agtcctcgaa tttcatcaat tagggattga gggaaagata ggttgtattc atgctttaaa
    18361 gccaggctat cctattgatg ggcaaaaaga aaatatttta gcagctaaac ggtatgatgt
    18421 ttataataat aaatttctat tagatggaac ttttttgggc tactacagtg atgacacgct
    18481 ttttcacttg aatcaaatat tggaagctaa taattctagc tttattattg aagatggtga
    18541 tttagaaatt atgaaaagag ctgcacctct taatacgatg tttgggatga attattatcg
    18601 ttcagaattt attcgtgaat acaaaggtga aaatagacaa gaatttaatt caacaggaat
    18661 aaaaggacag tcttctttta aattaaatgc tctaggtgaa tttgtaaaaa agcctggtat
    18721 tccgacaaca gattgggatt ggaatattta tcctcaaggg ttatttgata tgttgcttcg
    18781 tatcaaagaa gaatatcctc aacatccggt catttattta actgaaaatg gtacagccct
    18841 taaagaggtt aagccagagg gcgagaatga tattattgat gacagtaaaa gaatccgtta
    18901 tattgagcaa catttacaca aagttttaga ggctcgagat aggggagtca atattcaagg
    18961 ctattttata tggtctttgc aagatcaatt ttcttgggcg aatggctaca ataagcgata
    19021 tggtcttttc tttgttgatt atgaaacaca gaagagatat attaagaaaa gtgctctttg
    19081 ggtaaaagga ttaaaacgga attaaggtta gcgatttgac tgatgtttaa tatattttaa
    19141 ctatgagctt aaatttttta caggaggagt ttttatggat aagttagtcg ctgccattga
    19201 aaagcaacaa gggaaatttg aaaaaatttc tactaataat tatatgatgg ctattaaaga
    19261 tggatttatt gctactatgc ctttaattat gttttcaagc tttttgatga ttattattat
    19321 gattcctaaa aatttcggag tagagttacc gagtccagct attgcctgga tgaggaaagt
    19381 gtatatgcta accatgggag ttttgggtgt tattgtttca gggacagttg caaagtcatt
    19441 ggctggaaat gttaatagaa aaatgcctcc tggaaaggta ataaatgata cttctgcaat
    19501 gttggcggcc atatgtggct atcttgtatt aactgtaacg cttgtagttg atgagaaaac
    19561 gggatctaca agtttgtcga caaactattt aggatctcaa ggattgataa cttcgtttgt
    19621 cagtaccttt attactgtaa atgtttaccg attctgtatt aagcgagaca ttactattca
    19681 tttacctaag gaagttcctg gggctatatc acaagctttt agagatattt ttcctttttc
    19741 ttttgtttta cttattagtg gtttgttaga tattgtatct cggtttagtt tagatgttcc
    19801 ttttgcccaa gtatttcaac aactattgac tcctattttt aagggggcag aatcatatcc
    19861 tgctatgatg ttgatttggt ttatgtgcgc tttgctttgg tttgttggaa ttcatggacc
    19921 atctattgtc ttacctgctg ttacagcttt acaactgagc aatatggaag agaatgctca
    19981 acttcttgca aatgggcagt ttccttatca ttctttaacg cctaatttcg gaaattatat
    20041 cgctgctatc ggaggaacgg gggctacctt tgttgtacca tttattttga ttttctttat
    20101 gcggtctaaa caattaaaat cggtaggtaa agctacaatt actcctgttt tatttgcggt
    20161 gaatgaacct cttctatttg gtatgccagt tattttgaat ccctatcttt ttgtaccttt
    20221 tttgatgact ccaccagtga atgtatttct aggaaaggtc tttattgatt tctttggaat
    20281 gaatggattt tatatccagt taccttgggc ctttcctggt cccttgggat tgttaattgg
    20341 aacgaatttt caatctatct cctttgtatt tttatctttg attttagttg tcgacatatt
    20401 gatttatttg ccattctgta gagcgtatga tagacagtta ctgatgaaag aagatgttgc
    20461 aagctcaaat gatattattt tagaggagga gacaagtgaa ataatgcctg gtgagttaga
    20521 tgaaataaaa agtaaggagt tgaaagtact ggttctttgt gcagggtctg gaacaagtgc
    20581 gcaattagcc aatgcaatta acgagggcgc tcaattagca gaagttagag tgattgcgaa
    20641 ttcaggagcg tacggagctc attatgatat tatgggtgtt tatgatttaa ttattctggc
    20701 tccacaagtt cggagttatt atagagagat gaaggtggat gcagaaagat taggtattca
    20761 gatagttgct accagaggaa tggaatatat tcatttaaca aagagtccaa gtaaatcctt
    20821 acaatttgta ttggagcatt accaagctgt gtagtaagtt tttccatctt ttatttgagt
    20881 aaggattttg cttacagata ggcttggatt taaaaacgtt cccccttttt taatataaga
    20941 atccctcttt cacaattgta aaaagaggga ttttgtattt tatctcttag accaagttct
    21001 cttcataaag agaaggagga ttgggtaaat ctccaagcgc cctgcaatca ttgcaaagga
    21061 taggagaatt tttgagatgg gactaaagat tgagaaacta gaagtggttc ctagaatagg
    21121 cccgatatta ttgaaacagc taaagacagc gctggtcacg accagaaaat cattgctatc
    21181 taggctgaca ataaagataa gcgctagcaa aatcatagca tagatgacaa agtacttgag
    21241 aatcttatgc tgggtatctt tatcaatcac cgtttgattc acatggaggg tcaaaacacg
    21301 gtggggcgat aggattgaca aaatttggtt tttggcaatt tttgaaagga tgaggcctcg
    21361 aataatcttg agtccacctg cagttgatcc agcagagcca ccgattgcca tgaggaaaag
    21421 gaggataaac tgggagaaga ggggccagtt ggtaatatct ccatatccaa aaccagttgt
    21481 tgtaatgatg ttggaaacct ggaagaaggt catttcaaag ctctttgaaa accctgggta
    21541 gaggtagagg gtgttgaggc taatcaagcc tgtagaaacc agtacaatga ccaagtaagc
    21601 ccgaagttct tcgtcaccaa agaaggcctt gatgcgacgg agcatgaggt agtagtagag
    21661 gttgaaattt accccaaaaa ccagaactcc gatactgacc agataggtaa tcagtgagct
    21721 gccatagtgg gcaattccgt cgttatagac ggtaaagcct ccagttcccg ctgtccccat
    21781 agcaatgaca aaactatcgt agagaggcat accagctaga taatagatga tgacaaagag
    21841 ggagaagaga gctagataaa ggagatagag aatctgggca gtgtttttta gtttggatac
    21901 aaccttgcca aaaacaggac ctggaacctc agccttcatc acctctaggt ggctattttt
    21961 ggcattgtcc ataatagcaa gtgcaaaaac aagcactccc atccctccaa tcaagtgggt
    22021 aaaacttcgc cagaagagga gggaacggct gagaaccgaa acgtcgttca aaatacttgc
    22081 tccagtagtt gtaaacccag aactaatttc aaaaaaggca tcaataaggc tggggatttg
    22141 cccagaaaag acaaagggga gaccaccaaa gaaagaccaa aggatccaac agagggcaac
    22201 gatcaagact ccctccttgg cataaatccg ttgatttttt ggcttctgta aactccctga
    22261 accgcctaac aatacgagaa tccctatggt tgaaaagagg gctgtaaaga cttggctcga
    22321 ttcacggtaa tagacagcga tagccacagg aaccaagagg agaacagctt caatcaaaag
    22381 taactttgaa aggaggtaat gaatcatact tttattcatt tcttacctcg caatcaagtc
    22441 ataaatcttg gtgatgtttg gcaacaaggt tgttactagg agcttgtctc caacttccaa
    22501 catatcctcc ccagttggga aaatagtctt gccctttcga ataatggctg caataagaac
    22561 ctcttttttc aatttcagtt gagaaagagg tttggcagtc attttattgg cttccttgat
    22621 atggaattgc agggtttcga tttggccatt ggctagatgg tgcatggctt gaaggtctga
    22681 atactgggca ttaactcgac cacgaataaa gtgcataatc gtatctacag cgatgctttt
    22741 aggtgtgatg atacttgaaa aatcaggcgc attgataatc tcgaggagac tggtacgatt
    22801 gaccttagta atatttttct gtacacctac cctgtcaagg aacatagatg taatcagatt
    22861 ttcctcatcg actcctgtta gagtcgcaac ggcatcatag tgttgagcac tttcttccag
    22921 caggatatct tttgcggttc catctccttg aacgatgtag agatttggga atttctcgct
    22981 aaagaagctg gcgatttcag gattgatttc aatgactttt gtatcgatac gactatcttt
    23041 gagaatacca agtagataat aggcaattct acctgctcca acgatgagaa ggctcttcac
    23101 ggcgcgtgat ttaaaataat tatggaagag tatcatatcg acacggttac cagtgacaaa
    23161 gattctatct ttatcctgta cagtcatgtc accgcttgga atgataattt gatgatccct
    23221 ctctatcgca cagacaatga cattaccaaa ttttttacga aaatcagaaa tgggcatttg
    23281 gcaaagaccg ctggtggact tgacgacaaa ttccatgagg ctaacgcgtc caccagcaaa
    23341 gcgttcgaca gacagggcgt tggggaagtc aatgatattc gcgatagcgc gggcagccaa
    23401 gagctcagga ttaacgataa gagaaaaacc gagaatattc ttttccttga aataagagtt
    23461 agaatattca gggttccgca cccgaacgat agtttcttta gctcccattt tcttggctag
    23521 aactgctgca atcatgttga cttcatcgtg ctcagtcagg gcgataaaga tatcacaatc
    23581 ttggacgctg gcttgctcaa gaatggcaaa atcggccccg ttaccaagga taccaatgat
    23641 atcaaagcga ctgacaatat gattgagaac agcttcgtct tgctcaatca gcaaaacatc
    23701 atgcttttct gcaaccaagg agcgacagag ggcaaaacca acttttcccc ctccgacaag
    23761 gataattttc ataataaaac ctactttttc atgatgtaac tatcataccc tttttcaaga
    23821 aaaaatgcac ctactagcta ataacaagag cttttagtga aaattcgcta taaggtaaaa
    23881 ctatacccta accaattgaa atagctatta gcgactttct ctgaaatatg gtatgataaa
    23941 ggatatacaa ggagataaaa tgaacaataa tttactggta ttacaatcag actttggtct
    24001 ggttgatggt gcggtgtcgg ctatgattgg agtggcttta gaagagtctc caaccttaaa
    24061 aatccatcac ttgacgcacg atatcacgcc ttataatatt tttgagggga gctatcgtct
    24121 ctttcagacg gtggattact ggcctgaggg aacgacgttt gtatcggttg tcgatccagg
    24181 tgtcggttcg aaacgtaaga gtgtagttgc caagactgca aaaaatcaat acattgtcac
    24241 gccagataat gggacgcttt cctttatcaa gaaacacgtt ggcattgtag ccattcgtga
    24301 gatttctgag gtggccaata ggcgtcaaaa cacagagcat tcttatacct tccacggtcg
    24361 tgatgtctat gcctatactg gtgctaaact ggccagtggt cacattactt ttgaggaagt
    24421 agggccagag ctcagtgtgg aacagattgt agagcttcca gtcgtagcga ccatcataga
    24481 agatcatctg gtgaagggag ccattgatat tctggatgtg cgtttcggtt cgctttggac
    24541 ctctatcaca cgggaagaat tttacaagct ggaaccagaa tttggtgatc gttttgaagt
    24601 gaccatctat catgctgata tgctggttta tcaaaatcag gttgtctatg gcaaatcatt
    24661 tgcagatgtg agaattgggc aacccatcct ttacatcaac tctctctatc gtttaggttt
    24721 ggctatcaac caaggttcct ttgccaaggc ctataatgta ggtgtcggtt catcttggac
    24781 cattgaaata aagaaaatag aaggataaaa taggagaata gtatggaaat taaatttaca
    24841 attaaacaag ttgttgctgt cggaattggc gctgccctct ttgtcgtcat cgggatgatc
    24901 aacattccaa cccctgttcc aaatacaagc atccagcttc agtatgcggt acaagcgcta
    24961 ctttctatta tttttggacc gattatcggt ttgcttgtcg ggttgattgg tcatgcaatc
    25021 aaggactctc ttgctggtta tggtctatgg tggacttgga ttatcgctag cggactcttt
    25081 ggtttagttg tgggactttt tagaaagtac gttcgcgtga tcaatggtgt ttttgactgg
    25141 aaagatattc ttatttttaa cctcattcaa ctacttgcaa atgcccttgt ttggggtgtc
    25201 ttggcaccac ttggagatgt tgtgatttat caagaagcgg cagaaaaagt atttgcacaa
    25261 gggattgttg cgggaattgc caatggtgta tctgtagcta ttgcaggaac tcttctctta
    25321 cttgcctatg caggaaccca aactcgtgca ggaagtttga aaaaggacta atactcaatg
    25381 aaaatcaaag agcaaactag gaagttagcc gcaggttgct caaaacagtg ttttgaaatt
    25441 gtggatagaa ctgacgaagt cagctcaaaa catggttttg aggttgtaga tgaaactgac
    25501 gaagtcagta accataccta cggcaaggcg aagctgacgt ggtttgaaga gattttcgaa
    25561 gagtataaaa tgatggggaa ggctgggcaa ctagtctttt tcgatgttta tagattagtt
    25621 aggtaagtaa gctaggatag gaatcttttt ggcgctaaga ttttaagagg agagactgca
    25681 agaagggctt gatttatgat aaaatagaag tctaagaagc gaatgaagag agtaagatga
    25741 aagaagctat aattgagtgg aaggatttct ctttccggta tgaaacacaa caagaaccga
    25801 ccttgcaagg gatagacttg accatttaca agggagagaa agtcttaatt gttggaccat
    25861 ctggatcagg taaatctacc ttgggtcagt gtttgaatgg gattattccc aatatttaca
    25921 agggtcagac atatggagaa tttttgataa agggtcaaac agcctttgat atgagcatct
    25981 atgataagtc tcatctggtt agcacagttt tgcaggatac agatgggcag tttattggct
    26041 tgtctgtggc agaagatttg gcgtttgctc tggaaaatga tgtgacagcc ctagatgaga
    26101 tgaaaggtcg tgtttataaa tgggctgaaa agctggacct tcttccttta ctggatcagc
    26161 gccctcagga tttgtcaggt ggacaaaagc agcgagtcag tctggctggt gtcttgattg
    26221 atgaaagtcc gattctcttg tttgatgagc cactcgccaa tctagatccc aagtcaggtc
    26281 aggatattat cgaattgatt gaccagattc ataaggaaga ggggacgacg actcttatta
    26341 tcgagcaccg tttggaggat gttctgcatc gccctgtgga tcggattgtc ttgataaacg
    26401 atggtcgtat cctttttaat gggagccctg accagttgct tgcaactgat ttattgactc
    26461 aaaatggaat tcgagaaccc ctttatctaa cgactctccg tcaattaggt gtggacttag
    26521 tcaagaaaga acagttagcg aatctggata acatgtctat ctcaaaaggt caggttcagc
    26581 tgcagaatga actggcaaaa gaaactccag aattgcagtc actctttaaa ttagaggacg
    26641 tgtctttctc ttatgatgat agaccgattt taaaatccct acatttagat attaaaaagg
    26701 gtgaaaagat tgctattgtc ggaaaaaatg gagcagggaa atcaactcta gccaaggcta
    26761 taagtagctt tattcagacg gaaggacgct atctttggga aaaacaggat ataaaaggcg
    26821 attctgttgc agagcgggcg gaacgagtag gctatgtgct acaaaatcct aatcaaatga
    26881 tttcaaccaa tatgattttt gatgaggtgg ctctagggct ccgtttgcga ggtgtggatg
    26941 agaaggaaat tgaaacgaga gtctatgaaa ccttgaaaat ctgtggactt tatgaattcc
    27001 gtaattggcc tatttctgcc ctgtcatttg gtcagaaaaa acgtgtcacc attgcttcaa
    27061 ttttggtctt aggagctgaa attattctcc tagatgaacc gactgcaggt caagatcaga
    27121 agaactatac tgagattatg gaatttctcg aagagttaca tcaaaaaggg cataccattg
    27181 tcatgattac ccatgatatg caattgatgc tggattattc agaccgggtc cttgtcatgg
    27241 tggatggaga attgattgcc gatactgttc cagccagtct gttgagcgat cctgagctgt
    27301 tagtaaaagc caatctaaaa gaaacctcca tctttaactt ggctaagaaa ctagatgtgg
    27361 atccactgga tttaacggca ttttacaaag aaaggagaga gggatgcaag ctaaattaat
    27421 cggttaccag catagagata ctgtgattca tcgcttgtcg ggagctggga aacttctctt
    27481 tttcattctg gtatcattgg cggccatgat tagctatgat accagaatgc tcttgctgat
    27541 tgccatcttt tcagtctttc tcctctattt atcagaaatt cgctttaaag atgtttcctt
    27601 tgtagccgtt tttgcgacgg tatttgccgt tttaaacgtt ttgatggtct atctcttttc
    27661 tcccgagtat ggggttggac tttacggaga gagaagtgtg atttggcagg gaatcggtgc
    27721 ctacactctg accagtcaag agctctttta tctgctaaat ttggctatta agtacctttg
    27781 cactattcct ctggctatta tctttttgat gacaacccac cctagtcagt ttgcttccag
    27841 tttaaatcaa attggtgtgc cttacaagat tgcctattct gtcagtctaa ccttgcgcta
    27901 tattccagat ttgcaggaag aattctttac catcaagatg tctcaggaag cgcgtgggat
    27961 ggaattatcc aagaaagctt ctcttatgca acgaatcaaa ggcaatctgc tcattattac
    28021 tcccttgatt tttagctcgc tagaacgcat tgataccatt gcgactgcca tggagcttcg
    28081 ccgctttggg aaagagaaaa aacgcacatg gtatagttat caggccttga aaaaaggaga
    28141 ctatattacc ttgctcttgg caaccttgtt tttagtagtt agtttactac ttatcttgca
    28201 gaatcaggga cgattttaca acccttggaa atagcttgaa aaaattgaaa aaatcaagtc
    28261 atttctattg acaatgattc tgaaagtgtt atactaagaa agtagtttcg ctgatttact
    28321 tcaaacctgt tgtgaggtaa gttaacgatg ccttaaccac gctgtttgct gagcttgact
    28381 ccgggcagtg tggctatttt tttgcaatgg tgaaaggaag caagtcatga caaatcacat
    28441 tgtattattt gaacctcaaa ttccacaaaa tacaggcaat atcgcgcgta cttgcgctgc
    28501 gaccaattct cccctccaca tcatcaagcc tatgggcttt cctattgatg accgcaagat
    28561 gaagcgggct ggattggatt actgggataa gctagagatt tatttttacg agagcttaga
    28621 agatttcatg tctcagatga agggaaaact ctatctgatt tcgaaattcg cggaaaaaat
    28681 ctattctgag gtggatttat cgactgacga ggaccattat tttctctttg gacgtgaaga
    28741 caagggctta cctgaagatt ttatgcgaga acatcctgag aaggctcttc gtattcccat
    28801 gaatgatgaa catgtccgca gtctcaatgt gtctaatacc gtctgcatga ttgtctatga
    28861 ggctcttcgc cagcagaact ttgcaggtct tgaactcgtc catacctatg aagtggataa
    28921 attgaaataa aaaatgaaaa tgaacaaaat gcttgcgctt gcaagcgttt tttgttatga
    28981 taaaagagtc ttcagggcag ggtgtgattc ccgaccggcg gtgactttaa ctaggaaatg
    29041 atcttttcct tttatacttt gttgacaagc tttgcctaac cagaagttat gcctacagct
    29101 tgtcgcctag tctaaaagaa aaatctctat ttccttctct ttaaagaagt ccgcgagcgc
    29161 aagctgatgt ggtgagattc cacaaccgac agtatagtct ggatgggaga agacgaaaga
    29221 atagctttgt ctgttttgat agatttatag ccggattgta accacttgct ttgtttttct
    29281 ttaatagagt aagagggaac ttttgggata taaaaagtga gaatagatag aggaatcctt
    29341 tccaacttct tctgatttta tagaaaattg gaggaacctg ttatgacaaa cacacgtcga
    29401 ctttcgacca ttgcaattct atcagccatc tcatttgtgc tgatgtactt tgactttccg
    29461 cttttaccag cggcatcttt cctcaagatc gaatttagta tcttgccagt ccttgtgggt
    29521 ctggtggtca tggatttgcc tgctgctcta ggagtgctct tgcttcgctc actattgaaa
    29581 ttgcttctta acagccaagg agtgaatact tacattggtt tgccgatgaa tatcgtagct
    29641 ttgggagttt ttgtcatcgt atttgctttg atttggaaaa aggaacggac aacccttcgt
    29701 ttcctactag gctctctagc tggaactgtt ggtttaacct tggccatgtt ggttctcaac
    29761 tatgtttatg ctgttccttt gtacgctaag tttgctaact ttgatattgg aaaaattttg
    29821 ggactttcca actacctaat gaccatggta ttacctttta acttgattga gggtgtaatc
    29881 ttttccgttt cattctggtt gttgtacgtc cttttgaaac caaccttaaa acactatgaa
    29941 agataaacaa acatttttaa tgaagggcag ttttgccctt ttacttttcg ttattcttgg
    30001 ctatatggtc aaattttacc ctgaaatgtt ggtcaatttt gaccaatcga ttcagactgc
    30061 cattcgagga gacttaccag attatttgac tattcttttt cgagccctca cacgtctgat
    30121 tgatatccca gtgattatca cttgggttgt cattacagct tttgtctttt atcgtaagcg
    30181 atggaagata gaaagtttct tcatgctggg aaatctggct ttggcaggtc ttttaatcgt
    30241 gacctttaaa aatatctacc agcgcccacg accagctatt ttacatctgg tggaggagaa
    30301 gggattttcc ttcccaagtg ggcattctct agctgtaacg ctgatggtag ggactttgat
    30361 tgttattctt agtcagcgga ttaaagatca ggtctggaga aaaatcgtac aaatcgtcct
    30421 tggcctctac ctagtcagtg tgctggtatc aagagtctat ctgggagttc attacccatc
    30481 agacgtcctt gccagtctct gtgtgggctt gggagtcctg tttatcgagt ttccctttta
    30541 tgacaagctc cgcttccaat ggcgatttaa aggcaaacag aagtgagtat cgaattccct
    30601 tgagagaaag aaatgaaagt caatatagca gatcttcatc cgactcaact atatttatca
    30661 gaaaagaagt tgcaagatat tcagatgctt taccagtcgg cagaaacaat ccaagtcgat
    30721 ccaatcagta ttcttgcctt tggagattgt ttgctgatta cagatgggca tcacagggct
    30781 tatcaggctt tattggcagg tcgggatacg atttctgctg agtgggatag agatggtggt
    30841 gatgaactat atcatctcta tgcgcaagct tgcgaggaaa gaaagattta ctctgttttt
    30901 gatttaaaaa atcatatctt acctctttgg atcggcatgg tgagcttttt tggattgaag
    30961 tcaaggagaa gggagaatgg tttccaattg gggatattac actatctcag gataatctcc
    31021 ccattgtgat tgggaattcc gcttaccaac atcgaggact tggaaaaaag attctaagtg
    31081 ctttgattga attggctcga gtaaaaggat ggaaagaatt gagagtcaag gaaatctaca
    31141 cctacaatca tgcttctagg aggtgtttca agtcgcttgg atttgtggaa aatggagcaa
    31201 cagaaaaagg aaggagtttt atattggaat tagtctaatc ctgccacttt ctccccctta
    31261 acctttgact attccgcaaa attatcgtaa aataaagagt aaatgataaa atgaggtcag
    31321 agtctgttcg ctctggcgat agtagtataa atgaggagaa acgctttgga attagaagta
    31381 tttgctgggc aagaaaaaag tgaactatct atgattgagg tagcgcgtgc tatcttggaa
    31441 cttcgtggtc gcgatcatga gatgcatttt agcgatcttg taaacgaaat tcaaaactac
    31501 cttggaacat caaacagcga tatccgcgaa gctttgcctt tgttctacac agagttgaac
    31561 tttgacggta gcttcatctc acttggggac aacaaatggg gtcttcgttc atggtatggt
    31621 gtggacgaaa tcgacgaaga aatcatcgct cttgaagaaa atgacgacga tgaagtagca
    31681 ccaaaagcta agaaaaaacg tgtcaatgcc tttatggatg gtgattcaga tgccattgac
    31741 tacaatgcag atgatccaga agacgaagat gcatacgaag cagatccagc tctttcatac
    31801 gatgatgaaa atccagatga tgaaaaaaat gaagtggaag cttatgatgc agaaatcaac
    31861 gaaatcgctc cagatgactt gggagaagat gtggatctca acgaagacga cgacgagttt
    31921 tcagatgacg atgctgaaac cagcgaggaa taagagttag ctattgacaa ttatcctatt
    31981 tttaggtatc atattgtttg ggcacctctt ttagaggtcg gggctcccta gttcttaggg
    32041 agctattttt gttttttcaa gaagttatct tcttgtattt tatactcaat gaaaatcaaa
    32101 gagcaagcta ggaaactagc cgtaggctgc tcaaaacact gttttgaggt tgtagataag
    32161 actgacaaag tcaggaacac atatctacgg caaggcgacg ttgacgcggt ttgaagagat
    32221 tttcgaagag tattagttgt gaatctggtg cagtcgtccc agattattct tattagtagg
    32281 gtcttgtttt ctatatcccc tcgtagttaa caagaccttg agcattttag aaagaggaat
    32341 ctatgtctac gaaatatatt tttgtaactg gtggtgtggt atcgtctatt gggaaaggga
    32401 ttgtggcagc gagtctaggc cgtctcttga aaaatcgtgg tctcaaagta accattcaaa
    32461 agtttgaccc ttatatcaat attgatccgg gaaccatgag tccttaccag cacggggaag
    32521 tttttgtgac agatgacgga gctgagacag atttggactt gggtcactat gaacgtttta
    32581 tcgatatcaa tctcaacaaa tattccaacg tgacaactgg gaaaatttac agtgaagttc
    32641 ttcgtaaaga acgccgtgga gaataccttg gggcaactgt tcaagtcatt cctcatatca
    32701 cagatgcttt gaaagaaaaa atcaagcgtg ccgctctaac gaccgactct gatgtcatta
    32761 tcacagaggt tggtggaaca gtaggagata tcgagtcctt gccattccta gaggctcttc
    32821 gtcagatgaa ggcagatgtg ggtgcggata atgtcatgta tatccataca accttgcttc
    32881 cttacctcaa ggctgctggt gaaatgaaaa ccaaaccaac ccaacactct gtcaaagaat
    32941 tgcgtggctt gggaatccaa ccaaatatgt tggttattcg tacagaagag ccagctggtc
    33001 aaggaattaa aaataaactg gcccagttct gtgatgtggc accagaagcc gttatcgaat
    33061 cgttggatgt tgaacacctt taccaaattc cactgaactt gcaggcacaa gggatggacc
    33121 aaattgtttg tgatcatttg aaattagacg caccagcagc ggatatgaca gaatggtcag
    33181 ccatggtgga caaggtcatg aacctcaaga aacaagttaa gatttccctt gttggtaagt
    33241 atgtggagtt gcaagatgcc tatatctcag tggtcgaagc cttgaaacac tctggctatg
    33301 tcaatgatgt agaagttaaa atcaattggg tcaatgccaa tgatgtgaca gcagagaatg
    33361 tagcagaact cttgtctgat gcggacggga tcatcgtacc aggtggtttt ggtcaacgtg
    33421 gtacagaagg gaaaatccaa gccatccgct atgcgcgtga aaatgatgtt ccaatgttgg
    33481 gagtctgctt gggaatgcag ttgacatgta tcgagtttgc tcgtcacgtt ttaggtcttg
    33541 aaggtgccaa ttctgcagag cttgcaccag aaacaaaata ccctatcatt gatatcatgc
    33601 gtgatcagat tgatattgag gatatgggtg gaacccttcg tttgggactt tatccgtcta
    33661 agttgaaacg tggctctaag gctgctgctg cttatcacaa tcaagaagtg gtgcaacgcc
    33721 gtcaccgtca tcgttatgag tttaataatg ccttccgtga gcagtttgag gcagcaggtt
    33781 ttgtcttttc aggagtttct ccagacaatc gtttggtaga aatcgtggaa attcctgaaa
    33841 ataaattctt tgtagcttgt cagtatcacc ctgaactgtc aagccgtcca aaccgaccag
    33901 aagaactcta cactgccttt gttactgcag cggttgagaa cagcaattag caaaatcaga
    33961 acctttgaga aaaatctcag aggttttttg catacaatga tattgcagta tatctgaggt
    34021 aggagtcctc tgtatgtacc tgctaccgtt gaaattaata gcgactccct cttgccctgt
    34081 gctagtgaat ggatttatca gtatattgaa atgaaataaa atctgaacaa attaattcgg
    34141 aaagccaaat caatttctag caaagtttta ggaactggat tgtatagtga attgaaataa
    34201 gatatgaaca tctctatcag aaaagtcaaa tt
//
LOCUS       DAVKLX010000019        33902 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKLX010000019 DAVKLX010000000
VERSION     DAVKLX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.019"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(670..1005)
                     /locus_tag="VW325_000970"
     CDS             complement(670..1005)
                     /locus_tag="VW325_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734397.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(1096..2406)
                     /gene="der"
                     /locus_tag="VW325_000971"
     CDS             complement(1096..2406)
                     /gene="der"
                     /locus_tag="VW325_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9734398.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(2420..3133)
                     /locus_tag="VW325_000972"
     CDS             complement(2420..3133)
                     /locus_tag="VW325_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9734399.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(3130..4026)
                     /gene="dnaI"
                     /locus_tag="VW325_000973"
     CDS             complement(3130..4026)
                     /gene="dnaI"
                     /locus_tag="VW325_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9734400.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(4027..5196)
                     /locus_tag="VW325_000974"
     CDS             complement(4027..5196)
                     /locus_tag="VW325_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9734401.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEYPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(5197..5670)
                     /gene="nrdR"
                     /locus_tag="VW325_000975"
     CDS             complement(5197..5670)
                     /gene="nrdR"
                     /locus_tag="VW325_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9734402.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5810..6175
                     /locus_tag="VW325_000976"
     CDS             5810..6175
                     /locus_tag="VW325_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9734403.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            6180..6875
                     /locus_tag="VW325_000977"
     CDS             6180..6875
                     /locus_tag="VW325_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734404.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6892..7659
                     /locus_tag="VW325_000978"
     CDS             6892..7659
                     /locus_tag="VW325_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734405.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(8012..9211)
                     /locus_tag="VW325_000979"
     CDS             complement(8012..9211)
                     /locus_tag="VW325_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734406.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(9208..10101)
                     /locus_tag="VW325_000980"
     CDS             complement(9208..10101)
                     /locus_tag="VW325_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734407.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            10217..10297
                     /locus_tag="VW325_000981"
                     /pseudo
     CDS             10217..10297
                     /locus_tag="VW325_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10782..10995
                     /locus_tag="VW325_000982"
                     /pseudo
     CDS             10782..10995
                     /locus_tag="VW325_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            11410..12027
                     /locus_tag="VW325_000983"
     CDS             11410..12027
                     /locus_tag="VW325_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9734408.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(12347..13234)
                     /locus_tag="VW325_000984"
     CDS             complement(12347..13234)
                     /locus_tag="VW325_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9734409.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(13383..15320)
                     /locus_tag="VW325_000985"
     CDS             complement(13383..15320)
                     /locus_tag="VW325_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEU9734410.1"
                     /translation="MDYSKVAAEVIGAVGKDNLVAAAHCATRLRLVLKDEAKVNQAAL
                     DNNADVKGTFSTNGQYQIIIGPGDVNFVYAEIIKKTGLKEVSTDDLKEIANKDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLFGPQSIEDMYPAIKGFSAMIQ
                     LMSAAPFMFLPVLVGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDILGFHVTQT
                     NYAYQVIPVLVAVWLLANLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLV
                     VSDAITNAIVWLYNTTGAFGMGLFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGF
                     GDYIFVVASMANVAQGAATLAVYFLTKNAKTKGLSSSAAVSAFLGITEPALFGVNLKY
                     KFPFFCALAGSAIGAFVAGLTHVIAVSLGAAGFIGFLSIKAGSIPMYIIAEIMSFVAA
                     FAFTYFYGKTKAASVFADEAATATAETVTEPTVEAPVVEETDTLQNETLVTPIVGDVV
                     ALADVNDPVFSSGAMGQGIAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLI
                     HVGIDTVSMNGDGFEAKVAQGDKVKVGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYA
                     SVAPVATGSVAKGDAVIEVKI"
     gene            15696..17150
                     /locus_tag="VW325_000986"
     CDS             15696..17150
                     /locus_tag="VW325_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9734411.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            17131..18096
                     /locus_tag="VW325_000987"
     CDS             17131..18096
                     /locus_tag="VW325_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9734412.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(18131..19405)
                     /locus_tag="VW325_000988"
     CDS             complement(18131..19405)
                     /locus_tag="VW325_000988"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9734413.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(19405..20577)
                     /locus_tag="VW325_000989"
     CDS             complement(19405..20577)
                     /locus_tag="VW325_000989"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9734414.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETKSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(20708..20956)
                     /locus_tag="VW325_000990"
     CDS             complement(20708..20956)
                     /locus_tag="VW325_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734415.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIDELSSN"
     gene            complement(21024..21650)
                     /locus_tag="VW325_000991"
                     /pseudo
     CDS             complement(21024..21650)
                     /locus_tag="VW325_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(21712..22161)
                     /locus_tag="VW325_000992"
     CDS             complement(21712..22161)
                     /locus_tag="VW325_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9734416.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(22163..22921)
                     /locus_tag="VW325_000993"
     CDS             complement(22163..22921)
                     /locus_tag="VW325_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9734417.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(23036..25015)
                     /gene="pknB"
                     /locus_tag="VW325_000994"
     CDS             complement(23036..25015)
                     /gene="pknB"
                     /locus_tag="VW325_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9734418.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSAMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKAQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(25012..25752)
                     /locus_tag="VW325_000995"
     CDS             complement(25012..25752)
                     /locus_tag="VW325_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9734419.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIVDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(25767..27080)
                     /gene="rsmB"
                     /locus_tag="VW325_000996"
     CDS             complement(25767..27080)
                     /gene="rsmB"
                     /locus_tag="VW325_000996"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9734420.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(27073..28008)
                     /locus_tag="VW325_000997"
     CDS             complement(27073..28008)
                     /locus_tag="VW325_000997"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9734421.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(28021..30417)
                     /locus_tag="VW325_000998"
     CDS             complement(28021..30417)
                     /locus_tag="VW325_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9734422.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(30483..30797)
                     /locus_tag="VW325_000999"
     CDS             complement(30483..30797)
                     /locus_tag="VW325_000999"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9734423.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(30822..31448)
                     /gene="gmk"
                     /locus_tag="VW325_001000"
     CDS             complement(30822..31448)
                     /gene="gmk"
                     /locus_tag="VW325_001000"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9734424.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(31578..33191)
                     /locus_tag="VW325_001001"
     CDS             complement(31578..33191)
                     /locus_tag="VW325_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9734425.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(33535..>33902)
                     /locus_tag="VW325_001002"
     CDS             complement(33535..>33902)
                     /locus_tag="VW325_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEU9734426.1"
                     /translation="QIQNKIYHYLIKRINDKRVEGVKTFPDPREKSVSDLYNLSRKGY
                     FSDFEILKDIEEDIRGLYPEVDWTWFHDRSDDVIHRLLEHRTPNNIKTYFSKNEEDNK
                     LINEYILKAFDEDKLILKK"
BASE COUNT         9599 a         7674 c         6475 g        10154 t
ORIGIN      
        1 gaatgtgaac gaatcaatta ggggatctaa agcattttat accaatgttt tataagttgt
       61 attgtattat tccatattca atccactata caagatgaat atttctctca agtttcataa
      121 tcaaaataaa aatgactgta tctatagctc cataaaaaaa tcatagatac agtcattttt
      181 tagttcctac accttccttt atgcacacct ttagacttcc ctagttcccg tctcaagtca
      241 agcatttttt tgaaacagat atagtctaac cagtcactag caaaagcgat aagtagtaat
      301 aatagccaaa caccccaaga cttgatgtct gccacatttc tcaagacgat attgaacaac
      361 aggaccgaaa aaactgtcca agccagacta aaaagagcat agagggtgat gtaaatccag
      421 taaaaatagt aaaaaattga gaaaatctta ctatctctgt ttgccctttc aatccagctg
      481 aaaaagtaaa aacagggtaa taagattacc attttaaaca agtgttccat cacaaacctc
      541 ctcttttaaa ttgttttctt tagtttcgtt ctattaaatg ttattaccaa tataaacctt
      601 ggaatagcgt gtaataaatg attttagagg taccataagc cacctcctac aaatagaaac
      661 cgatataaat caatgccttc cacccttaga cttccctagt tcctgtctca agcgaaacat
      721 ttctttgaaa caggaataag ttaaccaatt cataccaata gctagcagaa taaaaagaaa
      781 ccaaatgccc cataacttga tatctgtcac atttctcaag acggtattga aaaacagaac
      841 tgaaacaact gtccaagcaa ggctaaaaag agaatagaag gggatgtaaa accagtaaaa
      901 ataataaaaa attggaaaaa acttactatt tctgttggcc ttctcaatcc agttatcaaa
      961 ataaaagtac ggtgctaaaa gtaagaattt aaacaaatgt tccatcaccg acatcaccct
     1021 ccttcttttg atagcgtttt ctattatttt attatatcaa aaaaatccgg aactgtcatt
     1081 ccagattcta cttttttatt tgcgttttct tgcgatgaga tgaatcggtg ttccttcaaa
     1141 aacaaaggcc ttgcggattt gattttccaa gaaacgcagg taagaaaagt gcatgagttc
     1201 ttcttcattg acaaagatga caaaggttgg tggtttggtt gccacttggg tcgcatagaa
     1261 aatcttgaga cgttttcctt tgtctgtcgg tgttgggttg atggcaatgg catccatgat
     1321 gacatcgttc aagacagctg atggaatacg tgtattttga ctttcgctga tttgcttaat
     1381 catctcagga agtttgtgga gacgttgctt ggttaaagct gatacaaaga taatcggtgc
     1441 gtaaggcagg tattggaact gctcacggat atcttcttcc cagtttttca tagtgtggtt
     1501 atctttttca agcgtatccc acttgttgac cacgataatc atccctttac cagcttcatg
     1561 ggcaaatcct gcgatacgct tgtcgtactc acgaatgcct tcttccgcat tgatgaccat
     1621 caagaccaca tctgaacggt caatagcacg catggcacgc ataacagagt atttctcagt
     1681 attttcataa accttaccag acttacgcat accagccgta tcaatcatgg taaactcttg
     1741 accatctgta tctgtaaagt gggtatcaat ggcatcacga gttgttccag caacaggact
     1801 agcaataaca cggtcttcgc ccaagatagc attgatcaag cttgattttc caacgtttgg
     1861 acgaccaatc aggctaaact taatgacatc tggattttct tcttcatatt catttggaag
     1921 gttttccaca atcgcatcta gtacatcccc tgtaccgata ccatggacag atgagatagg
     1981 cagtggttca cccaaaccga gggcatagaa atcatagata tcatttcgca tctcagggtt
     2041 gtctaccttg ttaactgcga ggataactgg tttgtgggtc ttataaagct tacgggctac
     2101 gtattcgtct gcatcggtaa ttccttcctt accggacacg acaaagacga taacatctgc
     2161 ttcttccatg gcaatttctg cctggtgctt gatttgttcc atgaaaggag catcaacatc
     2221 atcaattcct cctgtatcaa tcatgctaaa agaacgattg agccactcac ccgttgcata
     2281 aatacggtca cgtgtcactc cttcgacatc ttctacaatg gagattcgct caccagcgat
     2341 ccgattaaat agggttgatt tcccaacatt gggacgtcct acaatggcaa tagttggtag
     2401 ggccataatt tctcactttc tacaataatt tcttctgttc aagatttttt ctagttgagc
     2461 ttggttcagc ttgaccaaac tgttctgcta gacgctgact ccagcttgtg gtcgcacgcg
     2521 ccccagcata gtcagcctga acacggtcat aagcttggat tgcctcagtt gactgttctt
     2581 ggtattcttc ctcaaagaca acattctcta gtggcagtct cggtttcata tcatgatgtt
     2641 gatttggcac acccagtgcc atcccaaaga cagaataggt gtagtcaggt aggttaaaga
     2701 gctctgccac ttcttcagac ttgtatcgaa ccaaaccgat aatcacacca ccatagccca
     2761 agctttcagc tgccaacaag gcattttgtc cagcaagagc tgcatcgacc gaactaatca
     2821 agagaccttc cacaccttgg ggttggaagg tgtcggtatg aagtcgggct cccttttctg
     2881 ctcggttcaa atctccgaca aagagaagga aaacagcaga ctggcgaatg gcttcttgag
     2941 gtaccaattc atacaaggca tctttcttct cttgacttcg taccacaatc acagagtagg
     3001 attggaaatt cttccaagat gatgccatct gggctgctgt caaaatctca tttaagtcta
     3061 cttggggaat ttcttgctct ttaaacctgc gcactgaagt atgagccttc atcaatttaa
     3121 tggtttctgt catcgacggt ttactccttc taaacgagtc tcctcagcca aataacggat
     3181 gcgttccatg acccgtctgg cttcccaggt ttcgtcattt ccatgtttca ctttcgcaaa
     3241 atgcttctcc aaatcttcaa agttgaagtt ggatgtgaaa aaggtcggta aattttcctg
     3301 catccgatat tggagaatga cctgcaggat ttcgtcacgc acccaaacgg ttgattgctc
     3361 ggcgccaata tcatctaaaa tcaggacctc agacagctta atctcatcca ccaaggtctt
     3421 aacattgcca tcactgatag catttttgac atcaatgaca aagctaggat agtggaggag
     3481 agttgatgaa acaccacgtt tttctgataa atcatgagct aaggccgcca ccatgaaact
     3541 tttacccaca ccaaagtctc catataagta aagacctttt cgaatagctg gatattgctc
     3601 cacgaaggct agtagctttt caaaaactgg taagcgcccc aaatcatcca agtcaacttg
     3661 agccaaacta gctttcttga gactggctgg tagattgatt aacttgagac ggttcttaat
     3721 agccgcttct ttttcagccg cgattagctc aggagtttct tcatatgaaa catctgcata
     3781 gccatgattc ttaaccaaaa tcggcttgta gcctttggca atataatccg tatccccacg
     3841 gagaaacttg tcacgctcgg tgatgtactg attaaacttg gagatactgc gatttaattc
     3901 ctctggagtc aaggattctt gctggataaa agccgcaaca tcagggtcct tcatgatttt
     3961 ctggaccaaa tcttgataat aaaaacggct gggttgacgt ttgagtacgt ctccgacact
     4021 ttccatctaa tctcctcctt tttctaatcg agctaatagt tcttgcttct tacgttctag
     4081 ttccagacga gtttcctcgc tggtttcatt cttatattca ggattactcc atttaggaac
     4141 attggttttt actggggcag tctgattctg tttttgtgtt tttgctttct gtcctcgctc
     4201 acggatgcgc aagactgcct tttctgccga atgaatcttt tgataggcat agtcattggc
     4261 taccttcatg gcatatttct cattgatatt tgccgaatcg accttattaa aggtcaataa
     4321 aagaatgata ttgatgactt cgtccagcaa gcccaagcca gccatctgtt gcaagagttc
     4381 tctttctgtt tgggtaatgg ttcccttgcg tgtttgcttg atttctgcca agaactgcag
     4441 ggcagtttta cttttggctt ctttgataat ggttgtttcc ttaagactaa agtcagagga
     4501 aactggtttt tgagcaattt tttcacgcat gcgtttggtt gaaataacct gagaaacagc
     4561 tgttgacttg gccaattgat aggtttcaaa ccaagtccat ttcttctcct cggcaatagc
     4621 aaagaggttt aagacatcgg actgctcatc cgcaaaacga agtccatctc gagccatcag
     4681 ctggcgaaaa tgttccaagt caaaatcatt ggccactttc ttcttgagac caaggtcttc
     4741 ttgactgcct agttctgcca attctggaaa gacttgattg agtgagacag gtatttcttc
     4801 accatcagca ctttcaactt tcaaatcctc cacagcagag tcgccaatct ttttctctaa
     4861 gagtctgcga taaacaggat actccaagaa gtcttgacta gatagaggag catggagggc
     4921 tagctgataa acatccccct tttgatagag ggtcaagaga ttaaaagcag ataagatttt
     4981 caatgatttt atcagtctat ccatcccaaa gttgagatga ttgaggatac ttgaaaaaag
     5041 atattccttt ctaccattat cccaaaaact gattgtataa agataaaggc tcagtgcctc
     5101 ctgaccgata atcgggaggt agcactgtac cagagatgag gtatcttgcg acacccgatt
     5161 attctttaga taagaaaaac ggtcaattgg cttcatttat ctttcctttt tctttttaga
     5221 ggactgggtg atttgttgga gcaagctctc taactcactg acatccttaa aactacgata
     5281 gacactagca aaacgtacat aggtaatctc gtccaattca gccaactcct ccatgacgag
     5341 tgaaccaatg tcctcacttt gaatttcatt ttcatttcga ccacggagtt tctgttcgat
     5401 acgattgact accatgttga tttcatcact tgacacagga cgtttctggg ctgagcggat
     5461 aatcccatta aagattttat ctctggagaa ttgttcccgt gtgccatctt ttttaacaac
     5521 cactaaggtt ctttcttcta ctcgttcgta ggttgtaaaa cggtgttggc actcgtcgca
     5581 ctctcgtctt ctacgaatgg tgttcccttc ttctgcttgg cgactatcga taacacttga
     5641 cttggtagcc ccacattttg gacaacgcat cctttccctc cttatcgttt tcttttcatt
     5701 ataccatttt ttaagcgatt cccaaaacaa ttcttctttt tgcttgacaa gttttttgtt
     5761 ttgttgtatt atttaattaa gacaaaaggg taagagaaag gagactaaga tgtcctggac
     5821 atttgacaac aaaaaaccca tctatttaca gattatggag aaaatcaagc ttcagattgt
     5881 ttcccataca ctggaaccca atcaacaact tccaaccgtg agggagctag ctagtgaggc
     5941 tggtgtcaat cccaatacca tccaaagagc cttatcagac cttgaacgag aaggatttgt
     6001 ctacagcaag cgaacaactg gacgatttgt gactaaggat aaggagctaa tcgctcagtc
     6061 acgcaaacaa ttatcagaag aagaattgga acacttcgtt tcctccatga cccattttgg
     6121 ctatgaaaaa gaagaactac caggcgtagt cagtgattat attaaaggag tttaagccta
     6181 tgtcattact agcatttgaa aatgtatcca aatcatatgg agcaacacca gcccttgaaa
     6241 atgtttctct tgacattcca gctggaaaaa ttgtcggcct tcttgggcca aacggctcag
     6301 gaaaaacaac cctgattaaa ctaattaatg gcctcttaca accagatcaa ggacgtgtcc
     6361 tcatcaacga catggaccca agcccagcaa ccaaggccgt tgtagcttat ttgcctgata
     6421 cgacctatct caatgagcaa atgaaggtca aagaagccct aacctacttc aagaccttct
     6481 ataaagattt caatcttgaa cgcgcccatc atctacttgc agacctgggc attgatgaaa
     6541 atagtcgtct caagaaacta tcaaaaggaa acaaagaaaa ggttcaactg attttggtta
     6601 tgagccgtga tgctcgtctc tatgttttgg atgaacccat tggtggggtg gatccagcag
     6661 cccgtgctta tatcctcaat accattatca acaactactc accaacttct accgttttga
     6721 tttctaccca cttgatttct gatatcgagc caatcttgga tgaaattgtc ttcctaaaag
     6781 acggaaaagt cgtccgtcaa ggaaatgtag atgatattcg ctacgagtca ggtgaatcca
     6841 ttgaccaact cttccgtcag gaatttaagg cctaagcaaa ggagattatt tatgttttgg
     6901 aatttagttc actacgaatt taaaaatgtt aacaagtggt atttagccct ctacgcagcc
     6961 gtgctagtcc tttctgccct catcggaata cagacacaag gctttaaaaa tctaccttac
     7021 caagaaagtc aggctactat gctacttttt ctagctacag tctttggtgg cttgatgctt
     7081 acacttgcga tttcaaccat tttcttgatt attaaacgct tcaaaggtag tgtctacgac
     7141 cgacaaggct atctgacttt gaccttgcca gtttctgaac accatatcat cacagccaaa
     7201 ctaatcggtg cctttatctg gtcattgatt agcaccgctg tattggctct aagtgctgtt
     7261 attattctgg ctttaacagc tccagaatgg attcctcttt cttatgtgat tacatttgta
     7321 gaaacacatc tccctcagat ctttcttaca ggtatatcct tcctactaaa taccatttca
     7381 ggaatcctct gcatctacct ggctatttcc attggacagc ttttcaatga ataccgtaca
     7441 gcactcgctg ttgcagccta cattggtatc caaatcgtca ttggatttat tgaacttttc
     7501 ttcaatctta gttccaattt ctatgtcaat tcactggtag gactcaatga ccatttctat
     7561 atgggagcag gtatagccat tgttaaagaa ctcatattca tagctatctt ttatctcgga
     7621 acctactaca tcttgagaaa taaggttaat ttgctttaaa taatttttac ctagatatgt
     7681 aacatactca tagaacaaaa gagaccaggc aaaaagtctt taaaattaga aaacgcatag
     7741 tatcaagtgt taaaaatcct tgatattatg cgttttttgt gagctgactt atttcctttc
     7801 gctatatcgc aaaatgaaat aagaacggaa cgatgggatt ttggaattca aatcaattta
     7861 taagaatgtt ttagaagtaa tattatccta ttccagattc agttcactat acaattgagt
     7921 tttcaagcaa cctgtttaca taatgtgtac ataattaggt tcgtgattcc acccttttca
     7981 cctttaaaaa cctcgctttc gcaaggctct tctatttata agataaggca cgtttaaagg
     8041 ttttccaaat ccctaaatca tccgtttgaa gaacgagact agcatacatg cgtccgataa
     8101 atcctgttgc taccaccgca aaaatcactg taatagcaag tgaaatccat gcttctgctc
     8161 cccccgcata gccattaatc gttcgaaacg gcataaagaa ggtcgaaata aagggaatat
     8221 aagaaccaat cttcaagagg agattgtcac cagctgcacc tagagctgtc actccaaaaa
     8281 aaccacccat aatcaaaatc atcaaaggcg acaaggcttt ccctgagtcc tcaggacgag
     8341 aaaccataga tcctaggaag gctgccaaga ctacgtacat gaaaagactg atcaaaataa
     8401 agagcaaggt attcagtgag atagcatctc ccaagtgatc caaaatacca gactgagcca
     8461 agaatggcaa atctttaaag agcaaaacgg cagccagacc acctacaaca tagatcccaa
     8521 tatgcgttaa aatcactaga aacagagcca tcatccgcgc atagaaatag tgacttgccc
     8581 ttatgctaga aaaaacgact tccataattt tggtgccttt ttcactggca acttcctgag
     8641 ctgttacacc cgcataggta atcagaatca tataaagaaa gaatcctaag gcacctgctg
     8701 caattgtttg aataaacttt ttattttcct tggcttcatc aatcttttct gtgaattgaa
     8761 ttgtctgcgc taagcgtttt tcctgctctt gagacaagga agcagttgaa cgattaagct
     8821 gattttgcag ttcattgagt gtacctgtaa cctcaaattt aattccattt tcaagcgatg
     8881 tttcgccatg ataaactgcc tttagaacac tatcttcttg atcaatggtc aaataacctt
     8941 ttaatttttc ttctttaatt gcttctttgg cacttgcttc gtctttatag tcgaagttaa
     9001 caccatttac attcttcagt ccttctgcta cagatggcac tgttgtcact actgccactt
     9061 tattattttt agccatagaa gaaccttgga tatgcccaat tcctacagag attcctaaaa
     9121 agaggaacgg cgaaatcacc ataaagaaga aactccatga ctcgacatgt cgaagatagg
     9181 tttccttgat tacaacccac atatttctca tacttccact cctgattcta gtttaaagat
     9241 ttcatcgata gttggcgctt gttggtcaaa tgttgcgata tattgacctt gagtcaagat
     9301 tgggaagagt tcccttccag cgctctcatc ctccaaaatc aatttccaac tgccttgttt
     9361 ggtcaagctc acctgtttga catgaggaag attttccaat tcttccttgc ttcgttcact
     9421 tgaaacaaag agacgcgttt tcccgtattg attgcggaca tcctgaactg gtccgtgcaa
     9481 gaccacacgg ccatctcgga tcatcagaat atcgtcacaa agttcctcaa cattggtcat
     9541 gacatggtca gaaaagataa tggttgctcc gcgctctttt tcctgaaaaa tgacttgttt
     9601 gagcaattct gtattaactg ggtccaatcc actaaaaggc tcatccaaga taatcaggtc
     9661 tggttcatga atcagagtaa taatgagctg aatcttctgc tgatttcctt ttgacagact
     9721 cttgatttta tctgtcagct ttcctttcac ttccaacctc ttcatccatt gagggagttt
     9781 ttctttgact tctttggcat ccatgccttt tagagtcgcc aagtagcgaa cttgttcaag
     9841 aactgtcaat ttaggcatga gactgcgttc ttcaggcaga taaccaatcc gagcataggt
     9901 ctcctgacga atatcctgac catccagacc gatttctccc tgatattcta ggaatttcaa
     9961 aatactatgg aaaatcgttg tttttccagc accatttttt ccgactagtc ccaaaatacg
    10021 acctggtcgc gcttgaaagt caataccaaa caaaacttgc ttggagccaa aacttttctc
    10081 tagacttctt acttctagca tctttcacct ccgaaatttc ttgcactcat tatactcctt
    10141 tttgatagcc tttacaatgt ttttgtccat ttttagaaga ctattgctgt gtaaaatatg
    10201 gcctggagca cttttatact caatgaaaag caaagagcga actaggaagc tagccgtagg
    10261 ctgctcaaag tacagctttg aggttgcaga taaaactgac gaagtcagct caaaacactg
    10321 ttttgaggtt gtggatagaa ctgacgaagt cagtaactat atctacggca aggcgaagct
    10381 aacgtggttt gaagagattt tcgaagagta ttagtgataa atccattata cagtagcaaa
    10441 ctcaatttat accttccgct cctcaactgt ctatttttaa tcctgaattg ttatttgagt
    10501 aactcctttt tcctcataaa gttttcttcc tctaaaactt ctggaaaaaa gcgaatagtt
    10561 tcagacaaca tttttataag aaacaagttc atctgtcatt tcaagaagga gtaatccttt
    10621 atctactaat ggacggaacg gaattcaacc gcttgtccga tatattttct aaggattata
    10681 tagtaaaatg aaataaaaac aggacaaata gatcgggaca gtcaaatcga tttctaacaa
    10741 tgttttagaa gtagatgtat actattctag tttcaatctg ctatatctat tatgcacacc
    10801 cctataggat ctaatgaaaa tcacaacagg ctcattcata gatggttacc tcaggaaact
    10861 aagaaaacga ctacaaagga agtcgcattc atcgaaaagt ggattaacaa ctatcctaaa
    10921 aaatgcttga actacaagtc ccccagagaa gacttctgga tgactaactt gaacttgaaa
    10981 tttagcaata attaattcac catctaacta tatttagtaa ttatttcaga actgattaat
    11041 attaaaatta actaacaatt caaaggattc atactagcca taaattacgt ccatcagaga
    11101 gagactctta ctacttttat attttagtct ttctggcttc aggatacata taaactttag
    11161 ggaaaatgac tattcgaaag cgcgaatgcc tcaaaattat ctcagataag ctattcgaaa
    11221 cttagaatgc ttttaaattt atggaattgc gattattcga aacctagaat gcatataacc
    11281 tttagttgac agacctattc taagtctcga agggctattt actttctatt ccttatcaaa
    11341 aaagactcat tccccctttc tcctccaaaa tatggtatag tagaaatata ctatctatga
    11401 ggagtttaca tgtcacagga taaacaaatg aaagctgttt ctccccttct gcagcgagtt
    11461 atcaatatct catcgattgt cggtggggtt gggagtttga ttttctgtat ttgggcttat
    11521 caggctggga ttttacaatc caaggaaacc ctctctgcct ttatccagca ggcaggcatc
    11581 tggggtccac ctctctttat ctttttacag attttacaga ctgtcgtccc tatcattcca
    11641 ggggccttga cctcggtggc tggggtcttt atctacgggc acatcatcgg gactatctac
    11701 aactatatcg gcatcgtgat tggctgtgcc attatctttt atctggtgcg cctatacgga
    11761 gctgcctttg tccagtctgt cgtcagcaag cgcacctacg acaagtacat cggctggcta
    11821 gataagggca atcgttttga ccgcttcttt atttttatga tgatttggcc cattagccca
    11881 gctgactttc tctgtatgct ggctgccctg accaagatga gtttcaagcg ctacatgact
    11941 atcatcattc tgaccaaacc ctttaccctc gtggtttata cctacggtct gacctatatt
    12001 attgactttt tctggcaaat gctttgacac gtaaaaaatc cgtttggttt cccaagtgga
    12061 tttttaaagc gtagattaac tatagcttga tactaaatat actttggtat ggaaatcatg
    12121 catatttttc gatagtgagg cgaggactta cctagccttc ccgccgtgat agaaacacct
    12181 gaaatctaat ggtttcaggt attcggaaac tttgagccta gtgtctcaaa gtttaggtat
    12241 ggaatttcga agaaagtcgc taccgtccgt aatcacttaa ggaaaggctc aaaaatattg
    12301 ttttcaacca caaaatccgt ttggtttccc aagcggattt tgtgctttat tttgaaactt
    12361 cttttgcaag aacaaagttc ccaagtgtgg cagaaccatt tcctgcgact gctggcgtca
    12421 cgatatagtc acgcacatct ggtactggta ggtaaccatt aagaagagat gtaaatttct
    12481 cacggacacg gtccagcata tgttgttgag ccatgacccc tccaccaaag acaatcacgt
    12541 ctgggcggaa agtcactgtc gcattaaccg cagcttgagc gatatagtag gcttgaacat
    12601 cccaaacagg gttgttgagt tcaatagttt ccccacgtac acctgtacga gcttccaaac
    12661 ttggaccagc tgcataacct tctagacatc ccttatggaa aggacaaaca cccttaaact
    12721 ctttttcaat atccattggg tgtctagcaa cataataatg acccatttca gggtgaccca
    12781 caccaccgat aaactcacca cgttggatga cgcctgcacc gatacctgta ccgattgtgt
    12841 agtaaaccaa gttttcgata cgaccaccag cattgttacg ggcaaccatt tcaccgtaag
    12901 cagagctgtt tacgtctgtt gtgaagtaca ttggcacgtt tagggcgcga cgaagggcac
    12961 caagcaagtc cacatttgcc cagtttggtt ttggagtcgt cgtgataaag ccataagttt
    13021 ttgagttttt gtcaatatca atcggcccaa atgaaccaac tgcaagacca gcaaggttat
    13081 cgaattttga gaagaactca atggttttat cgattgtttc gattggagtt gttgttggaa
    13141 attgtgtttt ttctacaacg ttaaagtttt catcaccgac agcacagaca aactttgtac
    13201 cgcccgcttc caagcttcca tataattttg tcatgataaa cctcttgttt ttattttctt
    13261 tattatagca tacttcgaaa gtctaaatgt ctctattttt tagattttcc tctgtaaatc
    13321 ttactatcta ataaaaacga acaaacatgt catttgttcg ttttcacatt agagaggatt
    13381 gattagattt tcacttcgat cacagcatcc cccttcgcaa ctgaacctgt tgcgactgga
    13441 gctactgaag cgtagtcagc tgtatttgta acgataacca ttgttgtatc atcaagtcca
    13501 gctgcagcga ttttgtttga gtcaaatgtt ccaagaacat cgccaacttt caccttatca
    13561 ccttgagcaa cttttgcttc aaaaccgtca ccgttcatag atacagtgtc aataccaacg
    13621 tgaatcaaaa cttcagcacc atttcttgtt ttcaaaccaa aagcgtgccc tgttggaaag
    13681 gcaattgaaa cttcagcatc agctggtgca tagaccacgc cttggcttgg tttcacagcg
    13741 ataccttgtc ccatagctcc acttgagaag actgggtcat tgacatcagc aagagcgaca
    13801 acatcaccaa cgataggagt tacaagtgtt tcattttgaa gagtatcagt ctcttccact
    13861 actggtgctt ctactgtagg ttctgtaact gtttctgcgg ttgctgtggc agcttcatca
    13921 gcaaagacac tagctgcttt tgttttcccg tagaaataag tgaaagcaaa ggcggcaaca
    13981 aaggacataa tttccgcaat tatatacata gggattgaac cagccttaat agaaaggaat
    14041 ccaataaatc cagccgctcc caatgaaact gcaataacgt gagttaaacc tgctacaaag
    14101 gcaccaattg ctgaaccagc aagggcgcag aagaatggga atttgtattt caagtttacc
    14161 ccaaagagag caggttctgt aataccaagg aaggcagaaa cagcagccga actggacaat
    14221 ccttttgttt tagcattttt tgtcaagaag tagactgcaa gagtagccgc accttgtgct
    14281 acgtttgcca ttgaagcaac aacaaagatg tagtctccaa atccagtacc gttattattg
    14341 tatgcagaca acaattgtgt ttcgattgct gggaatgatt ggtgaagacc cgtcataacg
    14401 attaatgagt aagttccacc aaatagcccc ataccaaagg cacctgttgt gttgtacaac
    14461 catacaatcg catttgtaat cgcatcagac acaaccaaca taacaggacc gataactgta
    14521 aaggtaagga atcctgtaat catcactgat aaaagcggtg taaaagtgaa gtcaactgca
    14581 gatggcaatt tcttgtggaa gaatttttct aaattagcta acaaccagac tgcaacaagg
    14641 actggaataa cctgataagc atagtttgtt tgtgtaacat ggaaacctaa gatgtcccag
    14701 aaggcttctt taccacccaa atctggagtg gtcataatca taccgattgc cgcaccaagg
    14761 aattggtttg ctccaaagcg cttggctgct gaaataccaa ccaaaacagg gaggaacata
    14821 aatggagcag cagacatcaa ttgaatcata gctgagaagc ctttgatagc aggatacata
    14881 tcttcaattg attgaggtcc aaacaaatct ggtgatgtca agaagttccg taaagccatc
    14941 aagagaccac ctgcaactaa ggcagggata ataggaacaa aaatgtctga caaaagttta
    15001 atcaagtcca tcaatggatt gaactttttg tctttattag caatctcttt caaatcatct
    15061 gtcgaaactt ctttcaaacc agttttctta ataatttccg catatacgaa gttaacatca
    15121 ccaggaccaa tgataatttg atattgtccg ttagtgctaa aagtcccctt gacatccgca
    15181 ttattatcca gagctgcttg attcactttt gcttcgtctt tcaataccaa gcgaagacgt
    15241 gtcgcacagt gagctgcagc tacaagatta tccttaccga cagccccaat gacctcggca
    15301 gcaactttac tataatccat agcaaaaatc tccttatata acattttgtt cttgtttgaa
    15361 agcgatttta ttcgccctta cgactattat tttatcatgt tttaaaaata tgtcaagcgt
    15421 tttgcagaaa aaatattcta tccacttagt gggcaagttt tagattgatt gtttttgact
    15481 gtttcatcag gaattccttg aaaaataagt ttgaaacctt ggttaaaaag tcttttattt
    15541 cattatcttt cacattttcg taaaaatttg attgatttcc tgtatttatt ttatgataat
    15601 cgtttgacag tttttcctct tttttaacat aaaaggcttg cattttttat cgaaaacagt
    15661 tactattaaa agtgataaga tttttggagg aatgaatgga atggacaact gagcgtcgtt
    15721 acagacttta tcaagattgg acgcaagaag aaattcaaca tataaaggaa aatatggcac
    15781 aatctccatg gcatactcat taccatgttg agccaaaaac aggacttctc aacgacccaa
    15841 atggcttttc ttactttgat ggcaagtgga tcctctttta ccagaatttt ccttttggtg
    15901 cagcccacgg tttgaaatct tgggcacagc tagaaagtga tgatttggtt cactttaaag
    15961 aaactggaat caaagtttta ccagatactc cattagatag ccacggtgcc tactctggtt
    16021 ctgccatgca atttggcgat aacttattcc tattttatac aggaaatgtt cgcgatgaaa
    16081 actggatccg tcacccatac cagatcggtg ctttgatgga caaggagggt aagattacaa
    16141 agattgacaa gatcttgatt gaccagccag cagactctac tgaccacttc cgcgatccac
    16201 aaatttttaa ctttaaaggt caatactatg ctgtcgtcgg tggacaggac ttggagaaaa
    16261 aaggcttcgt ccgtctctac aaggctgtca ataacgacta cacaaactgg caagcagttg
    16321 gcgaccttga ctttgctaac gaccgtactg cctacatgat ggaatgtcct aatctggtct
    16381 ttgtagagga acatcctgtc cttctctact gtccacaagg attggataag aaagttctag
    16441 actacgataa tatctttcca aatatgtata agattggggc ttcctttgac cctaaaaatg
    16501 ccaaaatggt agatgtgtct caacttcaaa acatggatta cggtttcgaa gcctatgcaa
    16561 ctcaagcctt caacgctcct gatgggcgtg ctctagcagt tagctggctt ggtttgccag
    16621 atgtttctta cccatctgac cgttttgacc accaaggaac cttctctttg gtcaaggaac
    16681 tcactatcaa agacgacaag ctctaccagt atccagtcgc tgctattaag gaccttcgtg
    16741 cttctgaaga agccttctca aaccgttccc aaaccaagaa cacttacgaa cttgaactca
    16801 acttggaagc taatagccag agcgagattg tcttacttgc tgataaagaa ggtaagggac
    16861 tttcaatcaa ctttgacctt gtaaacggtc aagtgacagt ggatcgtagc caggctggag
    16921 aacagtatgc ccaagaattt gggacaactc gttcttgccc tatcgagaat caggctacta
    16981 ctgctacaat cttcatcgat aactctgtct ttgaaatttt catcaataaa ggagaaaaag
    17041 tattttctgg tcgtgtcttc ccacatgcgg accaaaatgg tatcctgatc aaatctggaa
    17101 acccaactgg aacttactat gaattagatt atggtcgcaa aactaactga tgtcgccaaa
    17161 cttgcaggcg tcagtcctac taccgtttct cgggttatca ataaaaaagg atatctatct
    17221 gagaaaacca tccaaaaagt caatgaagcc atgagagaat taggctataa acccaacaac
    17281 ctggctcgta gtctgcaagg aaaatcagct aagttaatcg gcttgatttt ccccaatatt
    17341 tccaatgttt tctatgcaga attgattgat aaattggaac accaactctt caaaaatggt
    17401 tacaagacca tcatctgcaa cagtgaacat gattctgaga aggaacgcga atacatcgaa
    17461 atgttggaag ccaatcaggt agacggcatc atttctggta gtcacaacct aggaatcgaa
    17521 gactacaatc gtgtgacagc gccgattatt tcctttgacc gaaacctatc gccagacatc
    17581 cctgttgtct cctctgacaa ctatgctggt ggggttcttg ctgcccaaac cttggtcaag
    17641 acaggtgccc agtctatcat catgattaca gggaatgaca attccaattc gccaaccgga
    17701 ctgcgccacg ctggttttgc atccgtactc ccaaaagctc ctattatcaa tgtttccagt
    17761 gacttttctc ccgtcagaaa agaaatggaa atcaagaata tcttgacccg ggaaaaacca
    17821 gatgccattt ttgcttcgga tgatttgaca gctattctgg tcattaaaat cgctcaagaa
    17881 ttgggcattt ctgtcccaaa agagctcaag gtcatcggct atgatgggac ctactttatc
    17941 gaaaattact accctcaatt ggctactatc aagcaacctt tggaagagat tgcttgtctc
    18001 actattgatc ttctcttgca aaagattgaa ggcaaggaag tcgccacaac tggttacttc
    18061 ttaccagtta cgctattacc aggaaaaagt atttaaacac aagaaaactc agaccgattc
    18121 gtctgagttt ttatgatctt aaattttcga gatagcgctg ggctgtctct aggttaaagg
    18181 ttttatctga gatgaggcgc tctactaggg gagcaacttc agattcacta gccccagcta
    18241 ggagagctag ggatttggcc tgtagtttca tgtggccttg ctggatgccc gtacttacca
    18301 aggctttgag ggctgcaaaa ttttgagcaa gaccgatgga cacgataatc tgggctaatt
    18361 ctctggcaga aggatttcct agtagatcat gactgagagc tacacgtggg ttgaggccga
    18421 tagagccacc cttagtcgct acaggcatgg gcagggtcat ctcaccgacc aattcttctc
    18481 tttcaaggtc cagcgtccag cagctaagac cttgatagcg tccatctcga ctggcaaagg
    18541 catgggcccc agcttcgatg gcacgccagt cattaccagt ggcaatcaaa atcgcatcaa
    18601 taccattaaa aattccttta ttatgagtag cagctcggta aggatcagcc tgcgcaaact
    18661 gactagccaa cgcaattttc tccgcaatct ctcgtccttg atccttttgg cggctcaagt
    18721 agcgaaaggc gatgcgacag cttgcagtca ccagagaatc agtcgcgtag ttggacagga
    18781 ttcccatgag actctgtccc tgactgagtt cttctaagac tggtttcaag gcttccagca
    18841 tggtgttgag catattggca cccatggctt cctgggtatc gacatgaata taaacaacga
    18901 gaaagtctgg ttcgcctttt atctgctcga catgcagatc acgcgcccca cctccacgtt
    18961 taacgataga aggataggct tgattggcaa gctccaagag ctccgctttc ttgctggcaa
    19021 tcttctcttg cgctagttta ggattagcaa cttgataaag ggctacctgc ccaatcatct
    19081 ggcgctgatg gacttgtgca gtaaaaccac ctgcacgctt gatgattttg ctggcatagc
    19141 tggccgccgc aaccacagag ggttcttctg tcacataggg aacggtgtat tcctgaccgt
    19201 tgacaagtac ctccggaacc agcgaataag gcagagaaaa agttcccacc acattctcac
    19261 tcagctggtc tgccacagtc acactcatct gttcatcctt ctccagacta gcttgtctct
    19321 caggactaag gagcgcctga gcttttagca gctcgaggcg ctcttggtat gattttttag
    19381 aaaatccatt ccaacttatc ttcattattt ttcaaccttg ctataacggc gttggtggtc
    19441 aagaatttca accaaggcaa aatcttgatt ttcatagcca gcaaactggg cagattttgt
    19501 ttcatccaag tttacttcct caaaaaagac cttttcatag tctgcaacgg atagggcagt
    19561 tcgttggttg agcttgttca agcggtcttt atccaaataa gcttcatatc cttcaaccaa
    19621 ttcaccactg aagaactcag ccacagctcc acttccgtaa ctataaaggg cgattttatc
    19681 cccagctttc aagctatctg tattttccaa gagagacaaa agtccaagga aaagtgaacc
    19741 tgtgtagata ttccccacct tttgactgta gagaatagac tggtcaaaat gcttttgtaa
    19801 gaggtctttt ttctcttgag gcaggctctt atccatgatt tttttcaagc cttttagcgc
    19861 taatttagga taaggcaagt ggaaacaaac agccgcaaaa tcatccagag taagctggta
    19921 gcgtttttga tattcaagcc aagtcgtttt caaactatcc aagtattgtt gggtagaata
    19981 aacaccattt acataaggag ttgtcgagta atttggtcgc cagaaatcca tgatgtcacg
    20041 ggtctgagct acattgtcat tattaaaggc catcatgcgt ggattttgtg taatcaacat
    20101 agctacactt ccagcacctt gagttggttc tcccggagtt tcaataccat atttggcaat
    20161 atcactggca atgaccaata ccttggactc cggagaattt tccacatgca atttggcata
    20221 atggagggca gctgtcgccc catagcaggc ttctttaatc tcgaaactac gagcaaaggg
    20281 ctggatgccc agcaagccat gcacaaagac ggccgcagcc ttactctggt caattcctga
    20341 ctcggtagcc acaatgacca tgtcaacttc ttgtctttct tgctcagtta aaatagagtc
    20401 actagcactg gccgccaagg tcacgatgtc ctcagttagg ggcgcaatac tcaattcctt
    20461 gagtaagagt cctttactta atttttcagg gtcaattccc ctcgcttctg ctaagtcttg
    20521 taatttcaag acatattgac tggtcgcaaa accaatctta tcaataccga ttgtcatatt
    20581 tacctctgtt ttatcattca tgtaaaaaat cgttctatac tattttatca caaatggcag
    20641 taaaagagag aaaaaagact tgattcacca aatcaagcct cttattggtc atcattttaa
    20701 agaatgatta gttgctagag agttcatcga tataagtagc tttataagct ccattcacag
    20761 ttatcagctc ctggaggatc aaatttcctg agtaagtcct tcccatctca tctacaaatt
    20821 tttgataaaa ctgactggtc ggaatttctc tgacatcctt atcaaatgtc ttgtcaagtg
    20881 ttttactaac cttctcagca atcaattgat gctcttgcca tccactttga aactctgagc
    20941 ccgaactaga aaccatgact gggataaaca acaaggtcag tagacttaca gacaataagg
    21001 aaaatagtag acttcctgca aaactagaat cctagttcat gattgataat accaacaatc
    21061 aaattcattc gtaatccgaa gcgtttacga tgatttcgat aggttgttga aatcatttta
    21121 aacgttttta ctttggcaaa gatgttctca accttgcttc tctccttaga tagcgcatgg
    21181 ttacaggctt tatcttcagc tgttagcggc ttgagtttgc tggatttacg tggagtttgt
    21241 gcttgaggat atatcttcat gagcccttga taaccactgt caaccaagat tttaccagct
    21301 tgtccgatat ttctgcgact cattttgaac aacttcatat catgacaata gttcacagcg
    21361 atatccaaag aaacaattct cccttgactt gtgacaatcg cttgaggttc catagaatga
    21421 catttctatt ttaccagaat aattggctag ttgatttttt taggacgatt tatttttacc
    21481 tctgttgcat ccacaatcac cgtattctca gcactaagat gagtttttga aatcgtaaca
    21541 ccactttgaa caagagttac ttcaacccat tggctccgac ggattaagtt gctttcgtgg
    21601 ataccaaaat cagccgtaat ttcttcataa gtgcggtatt ctcgcacata tagaaagcgt
    21661 tgttaattta tttatctcat ttttcagaaa attcttttat ttctgtaaag tctacgatac
    21721 tcgatgtgtt tttatataat gatacagtct gagaatcact gttccgctag ccattccaat
    21781 agagattacc agagccaaca tgacaaccaa ggtcgcactt gccagtgctt tattatagtc
    21841 ccctgtcaca aaaaaggcag ttgttcggta ggagagataa cctggaacca gcggtgccaa
    21901 aatggccaag ataaagacca cagcaggtgt cttataaaga atacttaaaa tctggctgac
    21961 acaagaacca ataatggctg caatgaaggt agctacaatg acattggtcg gttccttgag
    22021 caagagatag attagccaga cagtcatgcc caaaatccct ccaggtaaga gcatagaccg
    22081 ttgcacattg agtacgatta aaaaagtgat aatggcaaga aaacttgcta ctgcttgtaa
    22141 taaaaaggtt gttagtgtca tattagttca tcaataccaa ggcgacagaa gttcctgccc
    22201 ctaaagcgag ggtaatgagc agggattcaa acatcttact cataccagag tttatgtggt
    22261 tggtcataat atcacggacc gcattggtca aggcaatgcc tggtacaaac ggcatgaccg
    22321 caccagctat aatcaaatct gccgttgaag gaaaacctgt gtagcgagcc caaaactggg
    22381 caattatccc aaagacaaag gctccagcaa aggctgtcac aaagggaatt cggataaatt
    22441 tttccacata gagggaaaag gcaaaaccaa ataaggtcgc cactcctgcc ccaagtgcgt
    22501 cgtagatatt tccgctaaac ataactgaaa agaaaggagc actaaaggtc gcagccagag
    22561 ttacctgcaa cttagtatag ggaaggggtt gagcttgcaa ggccgtcaat tgcttaaagg
    22621 ctgtttctaa gtcaatctgc cccccaacta gctgacgaga aatctggttc acatcgcaga
    22681 ctttttcgat gttataagaa gaggaggtca cgcgcttcat gcgcgaaata ttggtatttt
    22741 caatagagaa aaagatagcg gcaggcatgg caaggacatt gcaatccaca atcccctgcg
    22801 aatgcgcgat tcgaatcatg gtatcttcta cacgatggat ttctgagcca cttttaagga
    22861 gaatagtccc cgctagcata atcacatcaa tgacggcatt taattctttt gattcttcca
    22921 tgctttcctc cttttatcaa ctccctctat tctatcacaa atctggactc aaaaaaaatc
    22981 tttgccatga aatcatgaca aagattgatt actcattttg attatccatc tgcttttaag
    23041 gagtagctga agttgtttta ggtttgtaga ttgaaatctt gactctagtc ttatttaggt
    23101 ctaccttttc acctgctcta ggactttgtt caacaaccat gccttctaca ctacctgcag
    23161 gcgctgtcgt cacttctaca acttctatat tagcttcctt aatcccaaca atttgaatca
    23221 aattgttctt agtaaactcc aagctggaac caatgtaact cggcatggca acacttgtaa
    23281 cttttttagc tactgtcaag acaatttgag taggtttact cacatcataa gtcgttccgg
    23341 cacctggact ttgtttcata atcgttcctg gttcgctttc gctggactct tcttcctcta
    23401 tcttaatcaa attctcagga accttcttct gcttgagttc tgagattact tctgtagagt
    23461 tccgtccaat atagttccct aattgaatcg tcgtagcttt tttagctact gtcaaaacaa
    23521 tttgagttgc cttgctcaag tcataggtcg taccttctgg tagactttgc ttcaagaccg
    23581 ttccagcctc actctcattc gactcttctt cctcaatttt aatcaaatta tctggaactt
    23641 ttttctcttt taattccgcg ataacatcag aagatttccg gccgacataa ttactaattt
    23701 ggaaggattg tttgcctgat gagacaacca gattgatttt cgttccttct tttcgaccag
    23761 ttccagcgcc aggatctgta cggataatcc gcccttcttc caccttttca ctagcctctg
    23821 tcttctcctc accaatctca aaattggctt ttttgagcgt tgccttggcc tctgcaactg
    23881 tctgacctgc cacatctgga atggcaatgg ttgcaggagt tctggatagt atccaaataa
    23941 gagaagctgc caccaataca aggctggcca acaaaatcag gtaacgcatc ttaaatctat
    24001 gttttttcgg tgcttgtggt tggtaagttt cctctgtcac agcctggctt gggtttttga
    24061 ttgatttgtg ttctgtctgc gcttgagcct taggaataga tgtcaaggta ctctgagaaa
    24121 ccttcggcaa ggtcttggta tctgccttgc tcgtttcatc aaagattaac ttactttcat
    24181 ttctacgatt gtaggacaag ctactagaca agtccacata catcgctgaa accgagcggt
    24241 agcgattggt caacttttta gcagtcgcct tgataataac attttctaaa gcctgaggta
    24301 cagatggatt ttctgcaata acggacggca ggggtttctg gaaatgctgg agggcaatgg
    24361 tcaccgcgct atccccgtca taagggatat ggcctgtcaa catctcatag aaaataatcc
    24421 ccatggcata gatatcactc tgcacagtcg ccttcgaacc acgcgcctgc tctggtgaca
    24481 agtaatgaac tgagcccaac atcgagttag tctgggtcag acttgtctct gcaaaggcta
    24541 cagcaatccc aaagtctgtg accttggcag tcccatctgg tgtcaaaagg atattttgag
    24601 gtttcaagtc cctgtgaaca attcctcgag tatgggccaa gcgcatagcc aagagaattt
    24661 gtcccatgat acggactgct tcttcattag aaagaggata atgttccttg atatagcgtt
    24721 tgaggtctag tccagcaaca tactccattg caagatactg ttgaccgtct tcctcaccaa
    24781 tatctgttat ccgaacgata tgaggatggt ctagatctgc catagctctc gcttcacgct
    24841 gaaaacgagc tacagctatc gggtccgtct ggtagttggt cctcagaacc ttcactgcca
    24901 cttcttcccc atctaagatt aagtctttgg ctaggtagac atccgccatg cctcctcgac
    24961 caatctgttt gacaatccga tagcgtccgg caaaaatctt gccgatttgg atcattctgc
    25021 atcctcctcg ttcatagaaa caagggcaac cgtaatgttg tctaaacctc ctgcattgtt
    25081 agcaaaacga acaagtgtct ccgttttatc tgctaaagga atatcactgg ttacaatatc
    25141 acgaatctca ctgcctgaaa tcatgttggt caagccgtca ctattgagca agagatagtc
    25201 acctgactca aggataactg tcccaaaatc aggctgaatt tcatcttttt gcccaataga
    25261 ctgggtgata atattttttt gcggatgagc ttctgcctct tctggtgtca attgaccagc
    25321 cttgagcaat tcattaacca aggaatgatc gctcgtcaac tgatggtatt cttctccacg
    25381 aatcaagccg atacgcgaat caccaatatg agcatagata gcctgattat caacaatagc
    25441 aaggacttcc aaagtagttc ccatgcctct gtaagcttca tcctgaccaa gctggtgaat
    25501 cttttgattt tcaatttcta ggtaatgggc gaaccattca cgcacttcat tgactgtatc
    25561 gatctgggta tcaacccaag ctacacccag gtctgtgacc gccatttcac tagcgatatt
    25621 ccctgcacga tgacctccca tcccatcagc taaaataatc atggtacgtc cagctctatt
    25681 gacatagtgg ttgacatagt cttggttatt tgttcgtttc tgaccaacat ctgttaataa
    25741 tgaaatttcc atgtgtcagt tccttcctaa tccgatatct tgcgaaattg actgatgaag
    25801 aatccatcac ttccatacaa ttcaggtgta atgaggatac agccgtcttt catgatatcc
    25861 ttacattcat gttctagttt tacctgctcg aactcgggat gactctctaa aaaggcctta
    25921 acgacttgaa aattctcctc tgagacgata gtgcaggtgc tataagttat tataccacct
    25981 ttgcctagta tttgacaaac actacctaat atttctaact gaatttcctg caaggacgcg
    26041 aaatctgccg tttctttatt gtatttgata tctggttttc ggcgcaaaag accgattcct
    26101 gaacaaggag catccaccaa aatcttatca aaggaatcct ggtcaaaaaa ctcatgcacc
    26161 tttctggcat ccaatttttg agtttgaacc cgatctgcaa ctcccagacg ttgggcattt
    26221 tcttgaatta aatccaactt gtggtcgtac aagtccagag cagtaacctg acctgtcgta
    26281 agataagagg ctatatgggc tgttttccca cctggagccg cacaggcatc aagcactcgc
    26341 tcatcacctt gtaaatcaag cgtcggagca accaactgac tggactcgtc ttggatggta
    26401 atggctccat ccgcaaacaa attatgccct gcaaaatgcc cctgctcctt aaccagacca
    26461 gtggttgcta aaagggaatt attcgcctcc aacaaggctt ggatttcctc ttttcgactt
    26521 aggtctgtta cacgaatact ggctttgttt cgcactaaca ggctttcaaa gatggctttt
    26581 gctctctcct ctccgtattc ttccttgagt ttggcaacta gccaaactgg gagagaatag
    26641 gcaatggagt cacgcttgtt ttttcgcttg atgctagcaa tatctggcca gccttcacgc
    26701 aagatacggc gaaggacagc gttgaccaat ttttcactgc cttttttacg gagtttggcc
    26761 aattccactg cttcattgac cacagcatga tctggaatct tgtccaaata gcggagttgg
    26821 taggcactca tgagaagaag gacatagagc cagctgtcta actggtctct gtcttcgata
    26881 aagtgggata ggtaccattc cagagtcagt ttacgggcta ccgttccata gaccagctcg
    26941 gtcactaagc ccttgtctgc tgccaaaagt tgacttccct ttagatgctt atttaaggcg
    27001 atatttgagt atgcttggtt cacaaaaaca tcctctagca ctgctagagc taaacttcta
    27061 gccgtttcta ctttagtcac caaatcgttc tcctacagtc aatgtacgtc caactccgtt
    27121 gaggaaggaa gcaatgtcca tcttaggctt accagctggc tgcacttgtt tgagggatag
    27181 agccccttca gccgttgcga caatcaattc tttcttgccg atagagagaa tctcacctgg
    27241 atttccctga ccttctactg gtagggcttc ataaatctta aagcggtcgc ccttaaggaa
    27301 agtatgggca acaggccagg ggttcattcc acgaatttgg ttaaagagtt gacgattggt
    27361 tttgttccag tccagttttt cttcctctgg ctttatattt ggagagaagg taacctgact
    27421 cgtatcctgc ggttcaggtt tgatatcacc agcaatatag gcaggcagag tgtccaaaag
    27481 caaatcacga ccaactagcg ccaatttttc aaacaaggtg ccaacattgt cctcatctgt
    27541 gatcggaatg ctgcgacgag aaatcatatc tcctgcatcc atttccttaa ccatttccat
    27601 gatggtcaca ccagcttcct catccccttg aatcaaggca taatggatag gcgcaccacc
    27661 acggtgtcta ggaaggaggg aggcatgaac gttgacagca aagtccatgc tatcaaggag
    27721 tttgcttggg agaaactgcc caaaagcagc agtcacaatt ccatctgctc ctagcttcat
    27781 aagatcttcc atctctggac ttccagataa tttttcaggt tggtagatag atagtcctgc
    27841 ttccttggca gcctgcttga ctggggtttc ttggataact tttttacgac caacagcacg
    27901 gtctggctgg gtcacaacgg ctagaatttc gtaacggtca tctgtcaaaa gtccttttaa
    27961 gactgttgct gaaaagtcgg gggtccccat aaagattagt tttgtcatat cttctccttc
    28021 ttataaaaat tgctgcggct catggtcaat actgagacgg agctcactat tttcccgttc
    28081 ttgagtcaag gccaggacct ggttgagggt cggccccagc tcatcttcta aacggtattt
    28141 aattaaaatc tggtaatgat agaggttgtg ggtacgggca atcggttttg gcgttggccc
    28201 cagaatggga ctggtctctg acaagcctga ccgcaaaatg ttcatgactt cataggcacg
    28261 tttgaaaacc tcttcttctt tcttgtgaga aagggtaata ccaatcgtga aatagtaagg
    28321 tggatagccg agttgtcgtc tgattcccat ttcataggca taaaagcctt cgtaatcttg
    28381 atccttggca aatcgaatag catagtgctg cggattgtag gactgtatca agacttgacc
    28441 tgctttttca gcccgacctg ctcgacctgc cacctgagtc aagagctgga aggttctctc
    28501 agaagaacgg aaatcaggca gattcaaggc cgtatccgca tttagaactc cgactagggt
    28561 aacattggga aaatccaaac cctttgcaat catctgagta ccaagtaaaa tatccgcttc
    28621 ccctcgccca aactggtcaa gcaaggcttg gtgactgcct ttctttcgag tcgtatccac
    28681 atccatcctc aaaatgcgag cttggggaaa gagttctgct agctcatcat aagccttctg
    28741 agttcccgtc ccatagtaac gaatactgcg gctcttacag ttaggacaga cctgaggaat
    28801 atccttcgag aaaccacaat aatggcagtt catagtcttg gtatccatat gcaaggtcag
    28861 agaaatatcg cagttgggac aagtatccac cgtcccacac tcccgacaca tgacaaagct
    28921 agaataacca cggcgattga gcatgagaac cacctgctct tttttaacca gacggtcttg
    28981 gatagcctct agcaaaggag gcgtaaagtt tgacgtctca ttttgtccga tatagtctcg
    29041 aaagtcaatc acttgaacct cagggattgt agccaaagga ttggcacgtt gggttagacg
    29101 taagtgttga tagacgcctt tgccagcacg tgcccggctc tctaagctcg gcgttgcaga
    29161 tccaagtacc agagttgctt gattatactg agcccgtaaa atagctacct ctctggcatg
    29221 gtaacgggga ttgctgtcct gcttataagc cgcttcatgc tcttcatcaa taatcatgac
    29281 acccagattt ttcagaggag caaagatagc agatctggca ccaacaacaa cttgggcatc
    29341 gccacgctcc accttgcgcc attcatcata cttttcgcca ttggataatc ctgagtgaag
    29401 aatggctacc ttgtccccaa aacgtgctat aaaacgctcg gtcatctgag gagtcaagga
    29461 aatctcaggt accagcaaaa tagctgtctt acccttatcc agggcacctt ggataatctg
    29521 caagtaaacc tcggtcttcc cacttcctgt aatcccttga agtagaaagg gaggttgaga
    29581 actgccaata gaactcacaa ccgcatcacg cgcctgtctt tgttctggat ttaactccaa
    29641 aggtctactt gcttcaattc cttcaaaata agcagccgag cgttgaactt ccttttggac
    29701 tatggtaaca gcaccttgat ccacaaagaa gttgacttgc tctcgcgagt aggactctaa
    29761 caagctagcc aaggaagcgc tctctggatg agacagcaga taatctctca gttccaactt
    29821 tttcttggca cgtgtagaaa tctcaacacc ttctaattgg gcatggtcaa cctcatacca
    29881 agactgggtc ttgaccttct tttgatcgac tgcctgatat tccagaccaa gcaggccttt
    29941 tctagtcaaa cgcatcattt cagcttgctt ggcaaggtct agtgaagaaa aggctagcga
    30001 atcttctgaa ccaaacaggc gtactcgttc ttcctgactc aagccttcca gaggatagag
    30061 aatcttgtca tagctagaat tcagaaaccc tggaagcatg gccttgagga tagagatttt
    30121 gtaggagaag acagatttgc gtaactcctc agccagccag agttgttctg gcgtgagaac
    30181 aggagaaaaa tccagcacct ctgcaatatc ttttaaatct tgctccatct cttctccatc
    30241 tgattgggac ttcaaaccaa gaacaatccc ttgaatcagg cgattaccct taccaaaagg
    30301 cacatgaacc cgcatcccaa cttccagcat tccctcaaat tcctccggaa tcctgtaact
    30361 atagggctgg tccgtctgca tcaagggcac atctacgata atcttagcta gggccatctt
    30421 ctcacctcct ccttgtcagt acattcttgc aatagaaaaa ataagattga gtccccccaa
    30481 ccttaaattt tttcaccatc ttctttttct ttagcaattt gctctttgat tttcttttct
    30541 tcttcttctt tgcggcgttt ttcttcttcg atacggcgac gcactgcttc acgttttcct
    30601 tctggatctg ggtgaattgt aacgtttcct gattcgattt cttctaaagc gcgaagagtt
    30661 gatttttcag acttgaaacc ttgagttgct ggggcacctg cttccaattc gtgggcacgt
    30721 tttgcttcca agattacgag tgaatatttt gaaggaacct tgtcgagcaa ggtatcaata
    30781 gagggtttta acatcatttg cttgtaccta ttttctaaat tttatcgggt agttggagat
    30841 tttggtaaca tctcctgata gtgaccaatt acacgatcca cacagaagtg ttctgcttca
    30901 atcacacatt tgacacgttc agcagctagg ggcacctgat cgttgacaat cgcataatca
    30961 tactcacgca tgagggcaat ttcttccttg gccttttcga ttcgttgggc aatcacttct
    31021 gcactatctg ttccacgacc taccaagcga tcttgcaatt catccaaatc tggtggtgtc
    31081 aggaagataa agacagcatc tggaaccttt ttcttgacct gaagagcacc ctgaacttca
    31141 atttcaagga aaacatcgat tcccttgtcc aaggtttcat tgacataggt cagaggagtt
    31201 ccatagtagt tgccgacata ttctgcgtat tccaacatct gtccttgacg aatcagctct
    31261 tcaaattctt cacgagtacg gaagaaatag tcaacaccgt ccacttctcc aggacgttgt
    31321 gcgcgtgtcg tcatcgatac agaatattga aattggtttt cagaactctc aaaaatctct
    31381 cttctaaccg ttccttttcc aacccctgaa ggaccagaaa aaacgattag taagcctcgg
    31441 tctgccattg tgtctccttt tagtcaatct gtgaaataac atttctctag aataatggca
    31501 aaaagccaga ttatccttta cagtctttct atctagtgta acaaaaaagc agtaattttt
    31561 caactgctct ttcttattta tttagcataa tctactgcac gaagctcgcg aatcacggtt
    31621 accttgatat ttcctggata atcgagattg ttttcaattt tcttacgaac tttgtgagcc
    31681 aagattgtga ctttgtcgtc cttgattttt cctggattga ccatgatacg aatttcacgt
    31741 cctgcttgaa gggcaaagct agtttgcact ccttcaaagc cgttagcaat ttcttccaaa
    31801 tcatggagac gcttgatgta gctttcaaga gactcactac gagcacctgg acgggctgcg
    31861 ctcaaggcat ctgctgcagc gacgataact gctatcacgc tctcagcttc aacatctccg
    31921 tggtgactag caatcgtatt caccacaact gggggttcct tgtacttacg ggccaattcc
    31981 ataccgattt caacgtggct accttcaacc tcatggtcaa tggctttccc gatatcgtga
    32041 aggaatccag cacgacgggc aagagccgca ttttcaccaa gttcgctcgc catgatacca
    32101 gccaacttag caacctcaat cgaatggcgc aaaacatttt gtccatatga agtacggaac
    32161 tgcaaacgtc ccataatctt catcaagtct ggatgaaggt ttggcgcacc aatttcatag
    32221 gcagcagcct caccgtattc acgaatctta ttgtcaatct cttgacggtt tttctcaacc
    32281 aactcttcga tacgagctgg atgtatacga ccatctttga gcaacatttc catagtcata
    32341 cgggcaatct cacgacgaat cggatcaaat cctgacaagg tcaccacttc tggtgtatcg
    32401 tcgataatca catcgacccc tgtcaaactt tcaaaggtac gaatgttacg accttcacga
    32461 ccaataatgc gtcccttcat agtatcgtct ggcagatgaa ctgttgagtt tgttgactcc
    32521 gctacatatt caccagcgat acgttgcata gcttgaacca agatgtcctt ggccattttg
    32581 tcagaacgtt ccttgacctc ttgctcagct tcgcgaatgc gactggcaat ctccctggtc
    32641 aagttttcct ctgtctgagc caagataata tctcgtgctt ctgcctgaga cagagcacca
    32701 atacgctcta gttctgcttc tttttgtctt tcgacttcct ctaattgctc ttcacgcgca
    32761 tcaaggtttt tcgctctatc agaaatactt tgttcttttt gttcaagtgt ttgttcttta
    32821 ctcgtcaaat tgtcgtcctt acggtcaagg ctagtagctc tctctgtcaa acgactttcg
    32881 atttgtttga gttcttgacg ttctgatttg aattcagcgt ccacttcttc acggtatttt
    32941 ctggcttctt ctttggcctc caatagtgct tcttttttaa gagacttgct ttcacgttta
    33001 gcttcattaa caagtaaatc cgcttcacgc tcagcttgtc cacgtaaatt agttgcttct
    33061 tgttcagcat ttaaaagcat caactccgca gcttcctgag atgatttcat cttagctgag
    33121 atgctgacat atccaatgac taaaccaatg atgacggcaa aaacagcaat cgcaagcgac
    33181 atgatttcca tgtttttacc tcattttatt gttattccga atgacataca ttcttttaca
    33241 ttctaccata aaaaagtgat tttcacaaac ctaaaataga atatgttttg gggaatttag
    33301 tctgaatttt aggattacac ttccttggtt ttttaaggag ataagtcagt gtcgctatca
    33361 aaaagttcca ccgaatttct acaactatgg attttctatt gttgtgccaa atagtgttcc
    33421 actagattca aacattttgc tttcaagtct ttgaaaattc actttaggaa attacagctt
    33481 atcttcaaga tcaaatttgt cataaattaa ctttttttat ttagttgtat caatttattt
    33541 cttaagtatt aatttatcct catcgaaagc ttttaaaata tactcattaa tcaatttatt
    33601 atcttcttca ttttttgaaa aataagtttt aatattattg ggagttctat gttctagtag
    33661 acgatggata acatcatcac ttctatcatg gaaccaggtc caatctactt caggataaag
    33721 tcctcgaata tcttcttcaa tatcttttaa aatctcaaaa tcagaaaaat atccttttct
    33781 acttaagttg tataaatctg atacagactt ttctcttggg tctgggaaag ttttaacccc
    33841 ctcaactctt ttatcattaa ttcttttaat caaataatgg tagattttat tttgtatctg
    33901 ag
//
LOCUS       DAVKLX010000020        33667 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKLX010000020 DAVKLX010000000
VERSION     DAVKLX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.020"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            1..670
                     /locus_tag="VW325_001003"
                     /pseudo
     CDS             1..670
                     /locus_tag="VW325_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(735..2297)
                     /gene="guaA"
                     /locus_tag="VW325_001004"
     CDS             complement(735..2297)
                     /gene="guaA"
                     /locus_tag="VW325_001004"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9734427.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2439..3137
                     /locus_tag="VW325_001005"
     CDS             2439..3137
                     /locus_tag="VW325_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9734428.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3180..4076)
                     /locus_tag="VW325_001006"
     CDS             complement(3180..4076)
                     /locus_tag="VW325_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9734429.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(4085..5020)
                     /locus_tag="VW325_001007"
     CDS             complement(4085..5020)
                     /locus_tag="VW325_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9734430.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(5017..5742)
                     /locus_tag="VW325_001008"
     CDS             complement(5017..5742)
                     /locus_tag="VW325_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU9734431.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(5826..6461)
                     /locus_tag="VW325_001009"
     CDS             complement(5826..6461)
                     /locus_tag="VW325_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9734432.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(6463..7290)
                     /locus_tag="VW325_001010"
     CDS             complement(6463..7290)
                     /locus_tag="VW325_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9734433.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFESNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(7312..7452)
                     /locus_tag="VW325_001011"
     CDS             complement(7312..7452)
                     /locus_tag="VW325_001011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734434.1"
                     /translation="MYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEMNEHVGLK
                     WY"
     gene            complement(7498..7647)
                     /locus_tag="VW325_001012"
     CDS             complement(7498..7647)
                     /locus_tag="VW325_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734435.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPT"
     gene            complement(7648..8179)
                     /locus_tag="VW325_001013"
                     /pseudo
     CDS             complement(7648..8179)
                     /locus_tag="VW325_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            8822..9433
                     /locus_tag="VW325_001014"
     CDS             8822..9433
                     /locus_tag="VW325_001014"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9734436.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLKAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9478..10206
                     /gene="rlmB"
                     /locus_tag="VW325_001015"
     CDS             9478..10206
                     /gene="rlmB"
                     /locus_tag="VW325_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9734437.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(10245..11156)
                     /gene="trxB"
                     /locus_tag="VW325_001016"
     CDS             complement(10245..11156)
                     /gene="trxB"
                     /locus_tag="VW325_001016"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9734438.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(11222..11446)
                     /locus_tag="VW325_001017"
     CDS             complement(11222..11446)
                     /locus_tag="VW325_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9734439.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11524..12267)
                     /locus_tag="VW325_001018"
     CDS             complement(11524..12267)
                     /locus_tag="VW325_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9734440.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVVQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(12267..13067)
                     /locus_tag="VW325_001019"
     CDS             complement(12267..13067)
                     /locus_tag="VW325_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9734441.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSVGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(13225..13581)
                     /locus_tag="VW325_001020"
     CDS             complement(13225..13581)
                     /locus_tag="VW325_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9734442.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNREAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13575..14105)
                     /locus_tag="VW325_001021"
     CDS             complement(13575..14105)
                     /locus_tag="VW325_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9734443.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGEPQDLSNLLLAPIGTNFEKRVWSYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(14105..14599)
                     /locus_tag="VW325_001022"
     CDS             complement(14105..14599)
                     /locus_tag="VW325_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9734444.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14734..15180
                     /locus_tag="VW325_001023"
     CDS             14734..15180
                     /locus_tag="VW325_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9734445.1"
                     /translation="MKTTFSYPKWAEIPNIDPYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            15177..15824
                     /locus_tag="VW325_001024"
     CDS             15177..15824
                     /locus_tag="VW325_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9734446.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     NQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15845..16426)
                     /gene="pdxT"
                     /locus_tag="VW325_001025"
     CDS             complement(15845..16426)
                     /gene="pdxT"
                     /locus_tag="VW325_001025"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9734447.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(16427..17302)
                     /gene="pdxS"
                     /locus_tag="VW325_001026"
     CDS             complement(16427..17302)
                     /gene="pdxS"
                     /locus_tag="VW325_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9734448.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(17454..18833)
                     /locus_tag="VW325_001027"
     CDS             complement(17454..18833)
                     /locus_tag="VW325_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9734449.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            19127..20050
                     /locus_tag="VW325_001028"
     CDS             19127..20050
                     /locus_tag="VW325_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9734450.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            20110..20715
                     /locus_tag="VW325_001029"
     CDS             20110..20715
                     /locus_tag="VW325_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9734451.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20734..21978
                     /locus_tag="VW325_001030"
     CDS             20734..21978
                     /locus_tag="VW325_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9734452.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(22201..22458)
                     /locus_tag="VW325_001031"
     CDS             complement(22201..22458)
                     /locus_tag="VW325_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9734453.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22500..24536)
                     /locus_tag="VW325_001032"
     CDS             complement(22500..24536)
                     /locus_tag="VW325_001032"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9734454.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24748..25665)
                     /gene="glyQ"
                     /locus_tag="VW325_001033"
     CDS             complement(24748..25665)
                     /gene="glyQ"
                     /locus_tag="VW325_001033"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9734455.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(25858..26619)
                     /locus_tag="VW325_001034"
     CDS             complement(25858..26619)
                     /locus_tag="VW325_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9734456.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(26892..27734)
                     /locus_tag="VW325_001035"
     CDS             complement(26892..27734)
                     /locus_tag="VW325_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9734457.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYHAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(27848..29239)
                     /locus_tag="VW325_001036"
     CDS             complement(27848..29239)
                     /locus_tag="VW325_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9734458.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTPFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVLENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEHLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(29477..29941)
                     /locus_tag="VW325_001037"
                     /pseudo
     CDS             complement(29477..29941)
                     /locus_tag="VW325_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            30095..31072
                     /locus_tag="VW325_001038"
     CDS             30095..31072
                     /locus_tag="VW325_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9734459.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSNRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            31069..32151
                     /locus_tag="VW325_001039"
     CDS             31069..32151
                     /locus_tag="VW325_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9734460.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(32215..33245)
                     /locus_tag="VW325_001040"
                     /pseudo
     CDS             complement(32215..33245)
                     /locus_tag="VW325_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         9982 a         7509 c         6065 g        10111 t
ORIGIN      
        1 tgaaaatcat tactatggat atgtttagtc cttactatga cttagctaga caacttcgct
       61 ttcaaatttc taggctcagg ctgaaacagt ctcccagact gtttcactcc cgaatgctaa
      121 aatcgttctt gatcgctttc atattgtaca acatcttagc cgtgctatga gtcgtgtgca
      181 tgtccaaatc atgaatcagt ttcatcgaaa gtctcatgaa tacaaggcta tcaagcgcta
      241 ctggaaactc attcaacagg atagccgtaa actgagtgat aagcgatttt atcgtcctac
      301 ttttcgtatg tacttaacca ataaagagat tttaaacaag cttttgagct attcagaaga
      361 cttgaaacac cactatcagc tctataataa ggaaccagag aaatttttcg gactcattga
      421 ggacaatcta aagcaggttc atcctctttt tcagactgtc tttaaaacct ttctaaagga
      481 caaagagaaa atcgtcaacg cccttcaact acactattct aacgccaaat tggaagcgac
      541 caataatctc atcaaactta tcaagcgcaa tgcctttggt tttcggaact ttgaaaactt
      601 caaaaaacgg atttttatcg ctctgaatat caaaaaagaa aggacaaaat ttgtcctttc
      661 tcgagcttag ctgacttcaa cccactacag ttgacaaaga gcctgatttc aaagcatttt
      721 ttgttagttt aaaattattc ccactcaact gttgcaggtg gtttacttgt aatatcgtag
      781 acgatacggt taacatgatc cacttcattt acgatacgta ctgagatttt ttgaagtact
      841 tcccatggaa ttttggcaaa atcagcagtc ataccatcga tagaagtgat agcacggatt
      901 gcaatcgtgt agtcatacgt acgaccgtca cccataacac cgactgaacg aacgcctgtg
      961 ttaacagtga agtattgcca aatatcgcgg tcaagtccag ctttagcgat ttcttcacga
     1021 agaatagcgt ctgattcacg aacggtttca agtttctctt cagtgatttc acccatgaca
     1081 cggatagcaa gtcctggtcc tgggaatggt tggcgccata cgatatggtc tggcatacca
     1141 agctctgtac caagagcacg aacttcatcc ttgtaaagag tattgagtgg ttcaatcaat
     1201 tcaaactgca tatcttctgg aagaccaccc acgttgtggt gtgacttgat agtttgagct
     1261 gtatccgtac cagactcgat aacatctgta tataaagtac cttgagcaag gaatttcaca
     1321 tctttgagct tgcttgcttc gtcatcgaat acatagacaa actcgttacc gatgatttta
     1381 cgtttttgtt cagggtcaga aacgccagca agtttgtcaa ggaaacgttt agcagcgtct
     1441 gctttgacaa tattcaaacc aaacttacca ccgagcatgt ccataacttg atcagcttcg
     1501 cctttacgaa gaagaccgtg gtctacgaag atacaaatca attgatcgcc aatcgctttt
     1561 tggagaagaa ccccaacgac agatgagtca acaccacctg atagaccaag aaggacacgt
     1621 ttatcaccga cggtttcacg aatttttttg atctgcatgt caatgaaatt atccattgac
     1681 cagtcacctt tagccttaca aatgttaagg gcaaagttac gaaggatatc atttccgtat
     1741 acagaatgac gaacttctgg gtggaattgg ataccgtaaa tgtgtttatc tgggttttcg
     1801 atggctgcgt atgggcagtc agctgatgta cctgtacgaa caaagtcagc aggaatctca
     1861 gtaaccgcat caccatggct catcaaaaca gtctgttcat caggtgttga ttcaaaaagc
     1921 gctgatggtg tgtgagttag ggttgattga ccgtattcac gatttccagc atcacctgca
     1981 ggaacaactt ttcctccaag tttatgggtc aataactgca taccataaca gattcccaaa
     2041 attggaattc cgagttcgaa gatttctggg tcaatatcaa atgaaccatc ttcatataca
     2101 gaatttggac cacctgatag aataattcct acaggattga cttcacgaac ttcagcagct
     2161 gaaattttat ggctttttag ttctgaaaaa acaccaatct cacggatacg gcgtgaaatc
     2221 agctggttgt actggctacc atagtccaat acgatgattt tttctacatc ttgcaaatca
     2281 gttgaaatgt tgctcatctt tttcctttct caaaagaaat atcttttctc aatattctat
     2341 cacaaataag ggcgaattac caactaaaca aggcgaagtt tgactttttc tagtttattg
     2401 gggtacaata gagaaaagat agaaatgaag tgaaaaatat gctacctgct tatatgaaaa
     2461 tccatgatca gataaaaaag gatattgacg agcaccgttg ggctattggt gagaggcttc
     2521 ccagtgaaag agatttagct gagcagtttg cggtcagtcg catgaccctc cgccaagccg
     2581 tatctctatt agtcgaagaa ggcgtcttag agcgccgtgt aggaagcggc acctttgttt
     2641 ccagtactcg agtacaagaa aagatgcgag ggacaaccag ttttactgaa attgtcaaat
     2701 cccaaggtaa agttccctct agccagctca tttcctacaa aaaaaccatt cccaatgagc
     2761 aagaagttgc caagctagga atttttccaa cggacaatat tatccgaatg gagcgggtcc
     2821 gctatgccga ccaagttccc ctagtttatg aagttgcttc tattcctgaa aaattcatta
     2881 aggactttaa aaaagaagaa atcaccagtc atttcttcca aaccttgcaa aaacatggct
     2941 atcgtatcgg caaatctcaa cagaccatct atgctcgcct tgctaaagaa aagattgccc
     3001 actatttgga agttgaaaaa ggacatgcta ttcttggatt gacccaggtt tcctacctag
     3061 aagatggtac tgcttttgaa tacgtaaaaa gtcaatatgt aggcgaacgc tttgaatttt
     3121 atcttgaaaa taattagatt accacgcaaa aactctctgt cacggacaaa gagtcttttt
     3181 tatttttcta agaataaatc tttcaaagaa atcaaagtta cagcaaacaa tatcattgcc
     3241 ataccaataa aatcaatggg ataaaattgt tcattcatta ttaagaaggc aaagaaaacc
     3301 gccgatattg gctcaattga agccaacaag cttgacttga ccggtcctat cagactggct
     3361 cctttaagga aagctgtata ggcaaagaca gtcccgataa ggataatgcc tgcaaacgca
     3421 aggagaaaat caagactagt cgggatatcg gcttgtagaa cccctgtaaa aggaagggcg
     3481 accaaacctg ctatgaccat tcctacacca atgaccaagc tgctccccca ctttttaatc
     3541 aaggctatgg gtaaaatgat atacagagca taagtcaagg cagaaaagag accccagaac
     3601 agaccagcag gtgtcatgga taactggtcc aactgcccat gtgttgcgat caggaaggtt
     3661 cctccgatgg cgaatatgat ggaaactatc tctcccagtg tcggtgccac cctatccttg
     3721 atacagctat aaattaaaat tccgacagga caaacatact gaagcaccgt cgctgttccc
     3781 gcattggtct cctgaatagc agacagatag gcgaattggt tgagaaaaag accaatcaga
     3841 gcaaaaataa gaagagacag caaactcttt ctatccttta aaaagaccag tattttatcc
     3901 tttgcagtag cataagccaa gagcatgaga attccaccag cgattaaaag acgcaagttg
     3961 gtcaagacca gagccgaaat tccgtgtgcc attaggtatt ggccactcgt tcctgacaac
     4021 ccccaagcaa taccagccac aactgttagt aaagtccctt ttaaactatt tgacatgtct
     4081 ctccttactc ttctttgcga atcaagttca tcacttgatt gcggtctaat atccattgag
     4141 ctctctcgtc cttttcatag gtacgattcg ataggaaaat agtggcttct tgcttctgac
     4201 gattccacat gataaaggta cctgtatagc ccgtatggtc tagccaatct ccttccaaat
     4261 tccatgctaa agaacgttcc ttgtcatcca aaggagaaaa attttgattt aagtctcttg
     4321 caaaatcatc tgctaaatag tgttctaaaa agatttgtaa atcctttata gtcgaaaata
     4381 aaccagcact cccagcatgt ctacccagga gacgagcctt gggatcatgc actatgcctg
     4441 cctctacacc tctaactgtt ggaacagcaa gctcaactgg cccaaacttg gtttccgtca
     4501 ttccccaagg tttccagact tgatccttta aaatcacatc caaatcttga ttaaaaattc
     4561 tttccaaaat aaagcccaac agcaaaaaat ggacatccga ataaagaaag gctggctgac
     4621 ttcgtctgtt gagatgaaac atcgcttcct ttaattcagg ggctgttaaa agatcacgat
     4681 taggaataaa aggatcaagg tctgttgcat gagtcaagag ctggcgaata gtgatgtctg
     4741 gataatcact ctcaggtaaa aaatctatta ccagtctatc aatatctaat tgacctattt
     4801 cccacaagaa ggtacaaact gtgccaaccc caacaacctt gctgacacta gctaggtcat
     4861 aaactagtcc tgcctcagtc tgcaagccat gctctgggtc actctggcct aaatagaact
     4921 ctgtccattg attgtcctta aaatacgcaa aagaggctcc gggataaatt cctgcctcga
     4981 tttgttcttc tattttttta ataatcttgg tccacttcat acttcttcaa accacaattc
     5041 aacattattt ctatctttac aaaggaagaa tttttcagac ttaggaataa aatattcagt
     5101 agactcaaac ttctggcgaa gacttgctat gtctaattca ttgaccaaga acttgagcat
     5161 agataagtcc caggtaaccg tattgtccac agtcaaatcc tgcccctgag ctggaataaa
     5221 atcaagggat gccccaattt cagatgattc caagaaactt tcgatatcag ttgggagatg
     5281 taattccata gaaatctcaa atttacttaa agaaattgat gccaaatctg tttgaaattc
     5341 aggcttttct cctacttcta ctagacttgc tatgtcatct tccgcatgaa tcaaaatcaa
     5401 atcatcttct ggtgagaaaa tttcaaaagc gtagccattt tgacctttat ataatcgatg
     5461 aatcgaatct gttttagata agattccttc aatttctaag ggattttcca ccttgacaat
     5521 caatctagct agtttttttc ttccctctac cttacgagta cgcatactgg gagcttcttc
     5581 taaaaccagc ttttcaagac ccgtttggtc acctagtgac agaaaggccg attcttctaa
     5641 caaggccttc attccaaggg tttcaatata aaatgtttca tttaattttc tattattagc
     5701 ttttaaagta ggaataatcc gtacaatctg atttacattc ataaattcct ccgtcacttt
     5761 ttattttaaa ggattttaga attttttaca agatattatg ctcaaattta acattattta
     5821 gctatttagt aaagatagtc tttcaaggat tttgacaaag cctgataacc agcaatactg
     5881 aggtgcagtc catcagttgt atattctttt ttgagttggc ctgcttggtc tgtcaaacaa
     5941 tcaaatactg gcacaaattc cacctgcata taggcagatg caagctcttg ataggcttga
     6001 ttccagttct gaattttttc attcgagcgg atatagactg cctgctggta ctcctctccc
     6061 tcattgacag gcaaaatgga aagcaattta atctctgtca atggataatc gcgagcaacg
     6121 gattgaatga tagcttcgag attattgaga gcctcattca caggaacatc ctttccgata
     6181 tcatttgtcc caatcagaag aaaaatttta tctactgctc caccatatag atgagcatca
     6241 aggttctcta gtaacagtcc tgtctgatag ccacgaattc ctcgattgac aatcgtcttt
     6301 gaagtcccaa atagctcctg tagaggataa tactcgacaa tggaatcccc aataaaaaga
     6361 atgtttggtt ctacaacaga aatgtgattt aggtgacgat acttagtttg aattttttct
     6421 tgttccttta ggagccaatt ttctaataac tgtactgcca cttcattctc ctttttctaa
     6481 ccagttttcc aagacttgat aaactcctcc ttggctgttg gctggtgcta gagcagtcgc
     6541 cacagctttg gctttctcat cagcattttc catcgcatag gcaattccag ccatttcaag
     6601 catttcaaca tcattttcac tatcaccaaa agccatgatt tcttgggatt tcaagtccca
     6661 gcgcttgagt aattcctcca agccccatgc tttatgaatc ccagcttgga ggatatcaat
     6721 gcaaccatag ccactggata cagctcggac acggccatca aagagagcat tgatttcttc
     6781 caaaaccgaa ctcaaacgtt cctcaccaac aaccatgctc atcttgagca caccaccaaa
     6841 gaggtcagat gttaattcat ccacaaattg catccgttgg tagaattttt caatcatttc
     6901 tggagtcata aaactttcaa gatctgtaaa aatcgtacct tccttgacaa aatcaccctt
     6961 catccccgtt acaacaaact ggtcctgaca cgctcgaccc ttgaaatgag tcaaagcctt
     7021 gttgacaatg gcgtcatccc atgtctgagc ctgaatcaat tcattgcttt caaaaatacg
     7081 agcgccatta gcaacaacca gaaccactcg atccaccaag ggactcagta gttgtctcat
     7141 gcggtgaatt tcattgcccg tcgcaatgac aaaacgaatg cccctttgat ccaactgatc
     7201 taaaatcttt tccaatcgtg ggagatcaag ctgacctcta gcatccagca aggtcccatc
     7261 catatctgtc gcaattacct taatcatcat ttactttcct ctggattcaa gctaatacca
     7321 cttcaatccc acatgttcat tcatttcttt ataggctgta tcaattcgtt ccttagtcgc
     7381 ttcatctaac tggtcacgat gactgccatc ctctataaaa tcttccaaaa tataagtttg
     7441 gtaatgatac aagggatatc cttctggtga aaaggttccc tttggtgttc gattgaccta
     7501 agtaggatga gctttagctg ttccgatagt tgtttttcca tccttcttct tgatggtgac
     7561 atccatgaga acaccacgtt cagtccactt agcattctct tcatctccca tagattcaat
     7621 ccgttgattg gaaatgaagt tccccattta atagacaatg agcttcttat ctccatcttt
     7681 ttcaaccgtt tcagatggtt caacaacgtg aggatgccct ccaaagataa tatccgctcc
     7741 ccaatcgatc atcttgtgat aaagagcttt ttgttcttca gttggttcca atcgatactc
     7801 aacacccatc tgaagcatga taatggtgat atctgcttcc ttctctgccc gttcaatttc
     7861 agccttcatc ttatcttcgt ttaaatctga aagataacga ttatagtctt ccttagaaat
     7921 atactgctca attccattga aaccatagga ataggctaac aatgcaacct tgatagcatt
     7981 cacttcctta atgaccagcg gagcctgaac acgtggttcg tgcgtataaa ctccgattgg
     8041 agtgattcca gctttctcaa taatatcggc cgttgaaata actccctcaa gttgaaataa
     8101 ctccctcaag ttgcgaatcc aaaatatgat tatgagctaa atccagcaca tgataacctg
     8161 cctccttaat agcatccata acttcagcag gagcattaaa gagaagataa cccgctaaat
     8221 aatgatcctt attaatgatt ccttcaaaat caccaatagc taaatctgct gcttagtctc
     8281 cttgcttgag ccaaggagtc acatactcaa aattttcatg aaagtcatag gtaccgtctt
     8341 cttttttagc tgaaaagaaa agtccatcgt ggtagagcaa atccccgtgg gccataattc
     8401 tggcagtttt ttcctcttcc tactcctgag acttttgctt agtcccctct tgagaaatag
     8461 tatctcgttt ttgactagtc aagggattcc ttggaagctt tcaaacaaca agaccaagcc
     8521 cttcataaaa cgaaaagcta aaccaccctg atttcgattt tatcttcttt gtatcttcat
     8581 tctccctact ttcttatgca agccttttct ttttattata tcacagataa gtatttcttt
     8641 cacaattgaa ttgaacttcc catctatttt ctataaatcc taaatgccat aatgctttca
     8701 attcttgtca ttttgtgata tcatgtagag gaaatgaact aatccacagt ggcttattcc
     8761 aagtatacca cttgggcttt ggcagtagct aactgcacta aacataatat aaggagagaa
     8821 aatgtctgca atagaacgta ttacaaaagc tgctcactta attgatatga acgatattat
     8881 ccgtgaaggg aatcctactc tacgcgcgat tgctgaggaa gtcactttcc ccctatctga
     8941 ccaggaaatc atcctaggcg aaaagatgat gcaattcctt aaacattccc aagatcctgt
     9001 catggctgaa aaaatgggac tccgcggtgg tgttggactg gctgctcccc agttagatat
     9061 ctcaaaacgc attatcgctg ttttggtacc taatattgtt gaagaaggcg aaactccaca
     9121 ggaagcctac gatttgaaag ccattatgta caatccaaaa atcgtctctc actctgttca
     9181 agatgctgct cttggcgaag gagaaggttg cctgtctgtt gaccgtaacg tgcctggcta
     9241 tgttgttcgc catgcccgcg ttactgttga ctactttgac aaagatggag aaaaacaccg
     9301 tatcaaactc aaaggctaca actccattgt tgttcagcat gaaattgacc acattaacgg
     9361 tatcatgttt tacgatcgca tcaatgaaaa agacccattt gcagttaaag atggtttact
     9421 gattcttgaa taaagaaaat cccgttgcaa gacggggttt tgtgttataa tagaggcatg
     9481 aaaacaaatg atattgtcta tggtgtccac gccgttaccg aagccctcct tgcaaataca
     9541 ggaaacaaac tctacctcca agaagatctc cgaggtaaga atgttgagaa agtcaaggaa
     9601 ctagctacag aaaagaaggt atccatttct tggacatcaa aaaaatctct ctctgagatt
     9661 actgaaggtg ctgttcatca aggttttgtt ctacgagtgt ctgaatttgc ctatagcgag
     9721 ctagattaca tccttgcaaa aacacgccaa gaagaaaatc cacttctatt gattctagat
     9781 ggtctaaccg atccccataa tctgggttct atcttgcgaa cagccgatgc gaccaatgtt
     9841 tcaggtgtca tcattcccaa gcaccgtgct gtcggagtaa ctcctgtcgt tgccaaaaca
     9901 gccacaggtg ctattgaaca cgttccaatt gcccgagtga ccaacctcag tcaaacctta
     9961 gataaactta aggatgaagg tttctggacc tttggaacgg atatgaacgg tactccttgc
    10021 cacaagtgga atacaaaagg gaaaatcgcc ctcatcattg gaaatgaagg aaaaggtatc
    10081 tctagcaaca tcaaaaaaca agtcgatgag atgattacca ttccgatgaa tggacatgtt
    10141 caaagcctta atgccagtgt tgctgcggcc attctcatgt acgaagtttt ccgaaataga
    10201 ctataaaaaa gtttccagtc atctgattgg aaactttttt atgattaact atgttctgta
    10261 atgaatttat aggcttcttg accagcgata gctccatctc caaccgctgt tgttacttgg
    10321 cgaaggtctt tcaagcgaac atctccaact gcaaagatac cgtcgactgc agttttcatg
    10381 tggttatctg tcacaatcca tcctgcctga tcttggatat tcaattcttt aacaaaatcg
    10441 ctaagagggt ccaaaccaac atagataaag acaccaccga aggcttgttc tgtcacttga
    10501 cctgttttca cattttcaaa tacgactgat tctactcggt tttcaccctt gatttccctt
    10561 actacagaat cccagataaa gctgattttt tcattcgcaa aggcgcgatc ttgtaaaacc
    10621 ttttgggcac gaagttggtc acgacggtga acaatggtaa cagtcttagc aaaacgagtc
    10681 aagaagaggg cttcttcaac agctgaatct ccaccaccaa ctaccaataa atcttggtca
    10741 cggaagaaag caccatcaca cacagcacag taagaaacac cacgactgtt cagttcttct
    10801 tctccaggca ctcccaaagg acggtgttta gaaccagttg ctacgataac tgtacgtgtt
    10861 tcatatgttt ggtcatcagt catcactttc ttaaaatcac catggtcttc gacattttca
    10921 acataaccat aaatgtgctc cacaccaaga ttttcaagtg gttcaaacat cttttcagcc
    10981 aattcaggtc cactaatatt agcgtatcct gggtaatttt cgatatcaga tgtattattc
    11041 atctgaccac ctggcagacc accttcaatc aaagctactt ttagattgct tcgagcagca
    11101 tacaaggccg cagtcatccc tgcaggtcca gcaccgataa taatagtatc gtacatatag
    11161 attccttctt tcttgttgta actatcttta ttctaactct ttcttgtcaa tcacgcaagg
    11221 attatgcctt gaaaacaaga attaaactca taaccgcaaa ggataatact gggacaacca
    11281 agactccaat cataatcata tcatagagat gggcttggcg agccttggct gtcttatcaa
    11341 ctcccgacat ggctctcaga ccaatccaaa tccctaaaaa aatcagaaca aggatggtgg
    11401 tcaagatcaa actctcgaaa tataaagaaa atagttgcag tagcatgatt tctctcattt
    11461 ctatcttttt taaagagtaa actcagctag tccaactaac tgagttttcc tttatctatt
    11521 atatcaaata taagtccgtt tgtaactagc gaagaattct tttgtccgct cttctttagg
    11581 ggtgtggata atctcatccg gtgttccaga ctcgatgatt ttccccttat ctaagaagag
    11641 aatcttatca gccacttgga ctacaaagga catgtcatga ctaaccaaaa tcatggtttg
    11701 acctgactta gcagcatctg caatagactt ttctacttca ccaaccaatt ctgggtcaag
    11761 ggctgaagtt ggttcgtcta agagcaaaac atctggtttc atagcaagcg cacgcgctag
    11821 ggcaacccgt tgcttctgtc cacctgataa atggcgagga taatggtttt cacggtccga
    11881 aagcccaacc ttagccaact cttccttggc aatcttagtc gcttcttggt cagataattt
    11941 cttgacaaca accaagcctt ctttcacatt atcaagtgct gttcggcgtt caaacaaatt
    12001 aaactgttgg aaaaccatag acaacttacg acgtagggca aggatttctt cttgagtgat
    12061 tttagaaaaa tcaactgaaa aaccatcaat ctgaatagag ccactgtcag gtgtttcaag
    12121 ataattgaga ctgcgaagaa aggttgattt tccagctcct gaagaaccaa tcaaggctac
    12181 aacttcccct ttttgaatat ccaagtccag atgatccaag acagtctgtc ctgaaaagga
    12241 tttgcttaaa tttgaaatct taatcattaa cgaaggtctc ctttcacatc tgtttgcact
    12301 gtatcaggtg cagaaatagc catttttctc tcgatgaaac gaccgaggct ttcaattccg
    12361 atattgacca cccaataaac aagggcaacg gagatgaagc gttcaaaata gcgataatca
    12421 gctccaccta gaatctgagc ttgggcaaag acttccacaa cacccacact aaaagctaga
    12481 gatgttccct tggtcaaacc gatgagggaa ttaatcaagg ttggagtagc taccaccgct
    12541 gcattaggaa taatcactcg tcgataaact tgcgctcggg tcatacccag actgcgtgcc
    12601 gcctcaatct caccaggatt aactgagaga atggctgcac gaatggtttc actagcataa
    12661 gctgcctcat taaaggcaaa ggcgacaatc gcaaaagctg cagctggaat cgcattgata
    12721 ttgagaccag ttccccattg ctgattgagg gctttcaaag ccaaagggat tccgtagtag
    12781 gtcaacatga gttgcaccaa aatcggtgtc ccttttaaga aactaacaaa gaaggcctgc
    12841 aagggatata aaatcttgac acgattgatc ttcacaatgg caaaaagaag cgccaaaacc
    12901 aagccaaaaa gggcaccacc aattgtcaac ataattgttg ttggaagttg ttggacaatt
    12961 ctagggattc catcaaagac cgaacgtagg ctaaacagct tgccatccgg aatcaattgc
    13021 atcaagtttt ggtaccaatc tgatgctaaa atcgttgtaa cattcataaa aacatcctct
    13081 tctgataatg attcattcta ccatgcttct gccgtcaatg aaattatttg ctacggtcag
    13141 atacagactt tgtctaccta ctagtttatc atactttgaa acagggaagt tatatatttt
    13201 atctatcaaa gagatagata aaaactattt cagtcccagt tcttcataag attttcgata
    13261 accgatttgc ttaacagttc cattttctac taaaatgggc cgttttaaca acataccgtc
    13321 acttgctagc aactcagccg cttctcggtt tgacaaactt cctaccttat cttttagccc
    13381 taattcacgg tatttgatcc cactggtgtt gaaaaattgc ttcaattcaa atcctgaggt
    13441 ttctagccaa ttcaaaatga cttcttggct aggtgtttct tccacgatat ggacggcttt
    13501 atagtccaca cctaattgat ttaattcttg ttttgctttt ttacaagttg aacatttggg
    13561 gtattcgata aattctaaca tgtgcttctc cttctattgt ttctatcttt aaaatctact
    13621 ccttcatgct ccaagagcca agctttcttt tccactcctg cagcatagcc tgtcagacgc
    13681 ttgcctgctc ccaacacacg atgacaaggt actaggatag accaaggatt gcgtcccact
    13741 gctccaccaa ttgcttgagc agaagccact tgcaggtctt gagcaatttg tccataggtc
    13801 actgtctgac cataaggaat tccctgtaaa taggaccaaa ctctcttttc aaaattcgtt
    13861 ccaattggcg ccaagagcaa gttggataaa tcctgaggct cgcctttaaa gtaatcatct
    13921 aagcaagcaa taactgggtc taaaatagga tgactaacaa cttcttctat cgtttcatct
    13981 cctagtcccc tctcaaaatg cttctgctcc tgaacccaaa ttccatacaa ataatggtca
    14041 tcagctacaa gtgatagaat accaattggt gaggagtaga gtatttttac atacttcttt
    14101 tgcattattt ctttaatttt tgataggcca agcgttcgcc atctactggc accttaccaa
    14161 cttgtttaaa accaagtttt tcaaaaatat gttgcataac cttgttttca gcatgcgtat
    14221 ctgagcgaaa atcaagataa tcaaaacctt caatcaagcc ctctaagaag gtttgagcaa
    14281 ctccttttcc ctgcacatct gctgccacag caatacggtg aaagactaga tactctgact
    14341 ctccagcttg ccagtttccc tcataaatag cttcataggc ctcctctgga ctcttggtca
    14401 cagcagcata ggctagcagt tctccctctt ccaaggctac gtaggcttgc cctgagataa
    14461 tatcatcgat aataacatct gcatttggat agccattttg ccactggtca ctaccagcat
    14521 ctgctaaaca ttttttggca tcctccatca cctgcatgat ggcatctact tcatttggaa
    14581 aagctaaacg aatttccatc ttttttcctc atttctgact agtttctccc atcatagcat
    14641 aatttaagta tttctacaag cagttaaaac ttgactttgt gtttttgtta ttttataatc
    14701 tagttatcaa aactatataa aaaggagttg aaaatgaaaa ctaccttttc ctacccaaaa
    14761 tgggcagaaa ttccaaacat tgacccctat ctggaccagg ttttgctcta tgtcaatcag
    14821 gtctgcgccc ctatctctcc taataaagac aagggcctaa cagcatctat ggtcaacaat
    14881 tatgtgaaaa atggttacct gacaaagcct gacaaaaaaa aataccaacg ccaacagatt
    14941 gcccgtttga ttgctatcac aaccctcaag tctgtatttt ctattcaaga aatagcccag
    15001 acacttaata ctctacaaac tcaagcaagt tcagaccaac tctacgatgc ttttgtggac
    15061 tacatgaacc aaggaattga cccagctaac cctattatcc aaaccagctg ccaaaccgtt
    15121 aaactctatc atcaaactct agacttaatc gatcatactc aagaggaggt aatccaatga
    15181 acactagtct taaactcagc aaacaactca gttttggaga ggagattgct aatagcgtga
    15241 cccatgctgt gggtgcagtc atcatgctta tcttgctgcc tatttcatcc atctatagtt
    15301 atgaagcaca cggattttta tcctctatcg gcgtttccat tttcgtcatc agtctctttc
    15361 tcatgttcct atcatccacc atttatcact ctatggccta tggttcgacc cacaaatatg
    15421 ttttgcgaat cattgaccat tctatgattt acgttgccat tgccggctca tacacgcccg
    15481 ttgtcttgac cttgatgaat aactggtttg gctatctgat tattgtcatc caatggggaa
    15541 cgaccatctt tggtattctc tataaaatct ttgctaaaaa ggtcaatgag aaatttagcc
    15601 ttgctcttta cctgattatg ggctggttgg ttctggctat cattcctgcc attatcaatc
    15661 aaacgacacc cgttttctgg agtctcatgg taactggcgg actctgttat acagttggag
    15721 ctggatttta tgccaagaaa aaaccttatt tccacatgat ttggcatctc tttatcctag
    15781 ctgcgtccgc acttcaatac atcgctattg tttattacat gtaaaaaagt tgagaaattc
    15841 aatctcaact tttttcttta cacatattga taaagtactg gtgcaagcgc acatcatcag
    15901 tcaattctgg atgaaaagaa cttaccaaca tatttttttc ttgggctgca acaatttgat
    15961 tgttcactgt tgctagaatt tctacaccct caccaacact actgataatc ggaccacgga
    16021 taaaggtcat tggaatcttg ccaactccct tacattctgc ttccgtgtag aaacttccta
    16081 attggcgccc ataagcatta cgctcgacca ccatatccat agttcctaga tgactctctt
    16141 tctgagaagt gatttcctta gccagcaaaa ttaagcccgc acaggtccca aacactggta
    16201 agccagatag aatggcttct cggatgggaa gtagcatgtt ctggtcacgt aagagcttgc
    16261 ccatggttgt agactcacca ccaggcaaaa tcaaacccga caagtcactc tgatcttgct
    16321 gaaaatcatc tagatttctg agttctacac tctcgacacc taattgatct agcacttttg
    16381 catgttctgc aaaggcccct tgcaaggcca atattccgat tttcatctat tttcctcgtt
    16441 cagccatgag aatttggatt tcattttcat taataccaac catggcttct cctaaatctt
    16501 cagagatttg agctaggatt tgaggattac ggaagttagt cacagcctta acaatggcac
    16561 tcgctcgttt aacaggatct cctgacttga aaatacctga accgacaaag accccctctg
    16621 cccctaattg catcattaac gcagcatctg ctggcgttgc aacacctcca gcagcgaaat
    16681 ttacaactgg caattttcca tgttcatgaa catattggac caattctaca gggacttgca
    16741 aatccttggc agcaacataa agctcgtcct cacgtaagtt ttgaatgcgg cgaatttcct
    16801 gattcatcat acgcatatga cgaacagctt ggacgatatc ccctgtccct ggttctcctt
    16861 tggtacgaat catggaagca ccttcagcga tacgacgcaa ggcttcaccc aaatccttag
    16921 caccacagac aaaaggaact tggaattctt tcttgtccac atggaaacgg tcatcagctg
    16981 gagatagaac ttcactctcg tcgatataat caatttcaat agcctctaaa atctgagctt
    17041 caacaaaatg cccgattctg accttagcca ttactggaat actaaccgct tcttggattt
    17101 ccttaatcat ctttgggtcg ctcatgcggg aaactcctcc agctgcacga atatcagccg
    17161 gaattcgttc caaggccatc acagctgccg caccagcagc ttctgcgata cgagcctgtt
    17221 caggattctg cacatccata ataacaccac ccttgagcat ctgtgccaag tttttattta
    17281 gttcataacg attttcagtc atttgtttta tcctcatcat ttgtattggt agctaccttt
    17341 tcattttaag tcagaacaga atgaagcaca agataaaaaa actataattg tatggggaca
    17401 gattggctaa aaattatctg accttaactt aaggccagat agctcattca tttctatttt
    17461 tcagccgtaa gggcagccat tgtgatgtag ttgtatggtt tgttgaagtg tggcaagaag
    17521 aagaggtctg tcaatgccaa tttatcaatt gtcacatgct cttggatagc aagtgagaac
    17581 atgtggattc ccatgctaat tgcaatatca tgtgaaacca tttgggcacc aagaatttca
    17641 cggctatctt tgtcaaagac aatcttaatt gctacttcat ggttgtcatg tttcatgaat
    17701 tctggttttt gaagatcgtt aaagcctgtt tcagttgcgt tgtaaccagc agctttcgct
    17761 ttttcaagag tcaaaccagt tgaaaccatg tgaagaccgt agattgagat accatttgaa
    17821 ccttgaacac cgattccttc caattcatgt ccacaagcat tgtaggcacc aacgatacca
    17881 gtgcgaacag catttgaagc aagagcgata tagcttgtat ctttacgagc attgtcataa
    17941 acagtcgcac agtcaccaac agcgtaaacg cctgggatag atgtttcttg tttcttgtct
    18001 acaaggaagg caccgttgcg gaagagttcg atcttaccat ctgcaagggc tgtgtttgga
    18061 cggaaaccaa ctgcaaggat aaccatatcc acgtcaaagc tttctttgtc agtaatcaag
    18121 cgttcaactt taccgtcacc ttcgattgct ttaacagttt gacctagagc caagcggatg
    18181 ttgtgatctt ccaagttctt cgccatcatt tgtgtgaagt ctttgtcata gtaaccgttc
    18241 aagacagtat caacgatatc aacaaggaca acttcttttc caagacgttc aaaggcttca
    18301 gcaagttcaa caccgatgta accaccacca acaacggcga tacggtcgag gtgttggctc
    18361 ttgtcagaaa gtttattgat aacttcttca gcattttggt acaatttcac gaattgtacg
    18421 ttttcaagag ttgctttaaa ttcgcggttt cctttaacaa tttcaacacc ttcgattggt
    18481 ggcaagattg gtgtagaacc tgtagcgaaa atcaattttt cgtatgattc tttgtgctct
    18541 tttccttcaa cttccgctgt aactactttg ttatcatagt cgattgaaag aacaggtgag
    18601 ttcatgtaaa ctttagcacc tttagcttcc aatttttctt tatcagaata gaacaagcct
    18661 tcagcaccgt caatttgttc accaatccaa agagccattc cacatcctag gaaagagatg
    18721 ttagagtttt ggtcaaatac aacaatttcg ttctcatttc caaaattatc caacatggta
    18781 ttgatacatg ctgtaccagc gtggttagca ccgactacaa cgattttact cataaaaatt
    18841 tcctgccttc attctaaatt taccttacta gtataccatt taccgttagc gtttacaagg
    18901 ggggtactat tatttcagca ttttttatca aaaataattc cccatttatc ctgttttcaa
    18961 gcctgatgaa ttttatgagc atatttcttg actttttcca aattttgttt gtgaaaattc
    19021 taactttatg gtattataat aacatgaaaa tgaaatgtaa gggcttcaat ttagatgatt
    19081 atttgatatt acaacttatt ttctataaga gaaaggagtt ggtagcttga ctcttcgttc
    19141 acattctgaa cggctaatgg ggactactat cactatttca ttagtggatg agcaagccga
    19201 tatctttctc caaaaatcct ttgacttgct caaagaactt gaataccgct tcaacgccaa
    19261 tagtcaagaa tctgagttga tggaaatcaa ttatcaagca ggaatagccc ccgtcacggt
    19321 tcatccagac ctgtttgagc tgatttcact tgggttagag catagcctag cgctctctag
    19381 ccacctcaat atcagcattg gtcccttaat tcaaacctgg cgtatcggtt tttcagatgc
    19441 caaggtcgcg cagcctcaag aaattgaatc ggtgctgcct ctaatcaatc ctcatggtat
    19501 cgagttagat tcttctactt ctactgtgtt tttaaaacag aaaggaatga agatcgatct
    19561 aggttgttta gccaaaggat acagtgcgga taaggttgcc caatttctta ggaaagaggg
    19621 ggtgacttct gccttgatca atctgggagg gaatatcctg accattggaa aaaatcaggc
    19681 aagaggggat aacccatggc aaatcgggat tcaagaccca gccaatccta ggggaaatca
    19741 tttaatgacc atccctgttg tcaataaatc tgtcgtgact tcaggcattt atgaacgtca
    19801 cctgaccgtc gatggacaag attaccatca catttttgac agtcaaacag gatatcctgt
    19861 tgaaacggaa ctagcgagtc taacaatcat ctctgataaa tcagtcgatg gcgaaatctg
    19921 gacgactcga ctctttggag aaagacctgc ttctatcctc tggcaagtcg aaagtttgga
    19981 gggcatcgaa gtcatcctca tcgataaaga aggccaccta agctgttctt caggaattcc
    20041 tactctatag agacaactgt ttcaatggaa ttttaaaatt gtattatgta aaaagagaaa
    20101 ggaaatctca tgttaaaact tattgctatt gttggaacaa attcaaaacg ttctacaaac
    20161 cgtcaattgc ttcaatacat gcaaaaacac tttgctgaca aagctgaaat tgaacttgtt
    20221 gaaatcaaga ccattcctgt cttcaacaaa ccagctgaca agcaagtacc tgctgaaata
    20281 ttggaaattg ctgctaaaat cgaagaggca gatggcgtta ttatcggtac tcctgagtat
    20341 gatcactcta ttccagctgt tttgatgagc gctcttgctt ggttgtctta tggtatttac
    20401 ccacttttga acaaaccaat catgattaca ggtgcttctt acggtacgct tggttcatct
    20461 cgtgcccaat tgcaacttcg tcaaatcttg aatgctcctg aaatcaaggc aaatgttctt
    20521 ccagatgaat tcttgctctc acactctctt caagcattta acccaagtgg cgacttggtt
    20581 gaccttgatg ttatcaagaa attggatgcc atctttgatg acttccgtat cttcgtgaag
    20641 attactgaga aattgcgcaa tgcgcaagaa ttgcttcgca aagatgctga agactttgac
    20701 tgggaaaatt tgtaagatag gagaccaaaa agaatgaaat ttgttggact tgttggatca
    20761 aactacgatc aatcatataa ccgtaagctc ttggaattta tccgtcgcta cttcaaactc
    20821 aaatttgaat tagaagttct tgaaattgac gaagttccaa tgtttaacca agatgaaaaa
    20881 tgggatgaaa gtttccaatt acgttattta tataacaaaa ttactcgtgc agatggtgtt
    20941 atcattgcta ctcctgagca taaccacact atctcagctt cactcaaatc tgtacttgaa
    21001 tggctttcat acgaagttca tccatttgaa aacaagcctg ttatgattgt gggtgcatca
    21061 tactatgacc aaggaacatc acgtgctcaa gttcaccttc gtaaaatcct tgatgctcca
    21121 ggtgttaatg cctacacgct tccaggtaac gaattccttc ttggtaaagc taaggaagct
    21181 tttgataaca acggaaatat caccaacgaa ggcactgtta aattccttga aacttgctta
    21241 gataactttg ttaaatacgt aggagtcgtt tcgaaattga aaaaaccaaa accaatcgaa
    21301 tcagaagact tggattgtgg aaaaccaatc gctacaacca tcacagaagt tgaccctgac
    21361 gatccagaat gggtagaaaa agttgcagca ttcactggtg ctgtttctgg tgatacctat
    21421 gtcaaattgg accacggtat cctaacagtt aaccaaattg atatgttctt gaaagctatg
    21481 ccatttgaat tgacatacgc tgacgacaac aaccaattcc tctactacaa caacgctcac
    21541 caagatccag acactatgtt cgctaaacgt gtaccacctc aatcaggtag ccgtatgtcg
    21601 actgttcatg gttctcttcc accagcacgt atgaagaacg tagagtgggt tatcggaaca
    21661 cttcgcaacg gaaaccaaga atacgtccgt acgatcgttc caggttctcc tgcaggtgtt
    21721 atcaacactc acaactacca agcaatgtac tatcctgatg gatcatacgc tggtatcaat
    21781 gaaatcgtct ttaacttcca accatggctt gactggtacc taaaagagac tggtcaacgt
    21841 ttggtgggtg gtagcggacc atttgctcct gctgccggag gtcatggaga cgccgatgct
    21901 acttctggcg cttctgattc aggggatgct ggaggccacg gtggtgacgc agacgctaca
    21961 gccggtgcaa gttactaatg atagttttta ggaaccatta tggttccttt tttagcgaaa
    22021 aaagactagc aaacacaaac tgctagtctc tttgttatcg tttcatctat ctttgttaaa
    22081 agaaagaata catatactca atgaaaatca aagagcaaac taggaagcta gccgcaggct
    22141 gtacttaagt acggcaaggt gaagctgacg tggtttgaat ttgattttcg aagagtatta
    22201 ttatgaattt ggatcatcaa gactacggcc atgtaatcct ttttcacgtt gtacttggcg
    22261 tagtttttct ggtgtaacat cgtttccttc ttcgtccaca attttgattc cttcaatgtg
    22321 gtgacgaaca gcgcggcgat aaccttcgat gtactcctca cgtagtttgg cttgttccac
    22381 tttttcttct ggtgttaagc cttctgtttt tttcttttta gcaagctcat tgatacgagc
    22441 aatttttttc ggatccattt cttctcctct gtaataagat aaagtccgtt tatcaaattt
    22501 tatttagtgt taatttggtt aaaacaagca aacttagctg ctttcttggt tagttgtgac
    22561 aagattgcca aacgattttg acggacagcc tgatcttcag ccattaccat agtattttca
    22621 aagaaagcat caatgactgg gctaagcgca aagagttgtt tcaattgctg acttgcaggt
    22681 cctgataaaa cgagtgtttc tactgcttct gccaaagctt tttcttggtc attctcaaat
    22741 agtgctgaat caaccgtagc aaccccttct gccttctcag ccaggttaaa ggcacgagaa
    22801 agtgattcaa cagatggttt aaaatcttct tccttgctta cttctacgag agcacttgct
    22861 gcttccaaca tatctgccac aacaaagttt gaacctgcaa gaactgcttc tttgatatct
    22921 tttggagtag agcccatcat cttatcaaca cgagccttga taaagtccat aacctctgct
    22981 ttattttcat aagtcaaact gtcaaatttc aatgcataaa ggctatcaat cagctcatcc
    23041 atagcaatgt gccaaccaaa ggcatccaag atacgaacca caccttgagt tgcacgacga
    23101 agggcataag ggtcattaga acctgatgga atcaatccta ctgagaagaa actcaaaatc
    23161 gtatccaatt tgtctgcaat ggctagaact gcgccgacct tgctctctgg aagttctcct
    23221 tcagctgatg taggcatgta gtgttcacga atagcagctg ccaccgctgg agtttcacca
    23281 gcaagaaggg tgtatttttc acccataatt ccttggagtt cgtcaaattc accaaccata
    23341 cctgtcaaca agtcaaactt gtaaatggct gctgcacgag caaggtcaac tgtttcatcc
    23401 actgacaaac tagctttttc tgccaaaagt acagtgattt gacccgtacg aatcatgtgt
    23461 tcacgaaggg aaccaatctt ctcatggaag gtgacattgt ttaatttttc aacaagatct
    23521 gaaatcacca atttttggtc ttcacgccag aagaattctc cgtcttccaa gcgggctacc
    23581 aagacttttt catttccttt gatgacattt ttcaaacgct ctgcgtttcc gttacgaaca
    23641 gaaatgaagt ttggcaagag ttttccatct tgatcacgaa caacaaagta acgctggtgt
    23701 tccttcatag aagtcaccaa gacttcttct ggaacttcaa ggtatttagc atcaaaactt
    23761 cccatgaagg cagttgggta ttcaaccaaa ttcaagactt cattcagcaa atccgcatca
    23821 atttcgatac gtacaccatg ttttgcctca atttccttga tttggtcaac aatcatttgc
    23881 tcacgttcac atggatctgc gattacaaac tgcttacgaa ggtcttcttc atagctcaat
    23941 gctgactgaa ttttggtttc tttccccaag aaacgatggc cacggctcac acgacttccc
    24001 ttgatatcaa ggaaatccaa gtcaaactct tgctcatcca agagaacagt taaagtgtga
    24061 acagggcgga tgtattcaaa gctatttcca gcccagtgca tgctgacagg gaaagtcaat
    24121 gacttcaaga catctacaac accaggaaca atggcttcaa ctgattggcc aacttcttcc
    24181 ttagtgacat agacatattc ttcaccctta atttcacgga attcgatatc ttcaacagtc
    24241 aaacctttcc cacggacaaa tccttgagct gctttggtga agtttccatc actatccaag
    24301 gcaattttct ttgctggtcc cttgaaatct tctgtcaaat cagactgttt gtctgataaa
    24361 ccagtcacac gaacagccaa acgacgtggt gttgagaagg tttgaatggc ttcaaaagac
    24421 aggcggtttt ccttgaggaa ggctgccatt ttttcgccta gttgtttttc acttggtgtg
    24481 acaacatagg ctggtaattc ttcaagaccg agttctacta ataagttttt tgtcatgttt
    24541 tttctccgaa aagtatcttt ttattttcca gtttgcctta tgtgattggc acgcaagcaa
    24601 ccaacttcgt tgtttcattg ctaaaattgg agccaaaagt ctccaatttt gcctagtatc
    24661 cttagtttca ctaaggatac cttcatcact gcggcaactg gctcagggct cactctgttc
    24721 gcccattttc gtaatttgtc actctcttta ttctgcgtct tctgctagga gtttagctct
    24781 tgtttcttca tccaaaagtg ggtagcctag gcgtttgcgt tctgcgacaa aggttttggc
    24841 tacaacacgg gccaagttac ggatacgagc gatatagcct gcacgctctg ttacagatac
    24901 ggcaccacgc gcgtcaagca gattaaaggt atgtgaacat ttgagaacat agtcataggc
    24961 agggtgtacc aagccttctt ctaatgcacg accagcttct ttttcaaact tatcaaagtt
    25021 ttcaagcaac atttcttggt ccgaaatttc aaatgaatat tttgagtgct catactcagg
    25081 ctggataaag atttctccgt attttacacc atcagcccac tcgatatcat agacagagtc
    25141 tacttcttga atgtaagaag ccaagcgctc caaaccatag gtaacttccg cagtcacagg
    25201 gccagttgcc aatccaccga cttgttggaa ataagtgaac tgagtgattt ccattccgtc
    25261 aagccaaact tcccaaccaa gaccagctga accagttgat gggttttccc agttgtcctc
    25321 aacaaaacga atatcgtgct ccaaaggatt gattcccaat ttttccaaag actcaaggta
    25381 aagttcttgg atatttgatg gagaaggctt catgaccacc tggaattggt ggtgttggta
    25441 gagacggtta gggttttccc cataacgacc gtcagcagga cgacgtgatg gctctacata
    25501 agctgcattc catggctcag gtccgatagc acgaaggaaa gtgtaaggac tcattgttcc
    25561 cgcacctttt tcattatcat aagcctgcat aagcatacaa ccttggtcat tccaaaattg
    25621 ttgcaaagta aggatgattt cctggaaagt caatttctta gacattgttt actccttttt
    25681 atataaatta cttgcgacat gagtctgcct cgtcgatcat acgaggcaag acgagttagt
    25741 actgcgtcta gctcaggcac tctagtgctg agcgtggggc agtccgactg taaggaggtt
    25801 tctgccactg acgcagtgaa atgtcaattt cttagacatt gtttactcct ttagttttta
    25861 tatttctttt tcaattaatt tctgctgaac aaaccaatct aacaagggaa aagttttttc
    25921 tgtatttgcc cataaatgat aagaatctaa gaccgcttgt acactaccta aattttctac
    25981 caacttatct actgtcaaaa agctgcgcca gtattcataa aaaatacttg catagttctt
    26041 atcataagtc gaagaaccaa aatcattcaa tgaatgccaa ccatacttct tctgaaaaag
    26101 ttctacgaga gattgattac aaattttttc cgcttgaaat tcctcttctg tcaagaaata
    26161 cttgcgacta atatattcaa ccatcccctc ttcgaaccag atataagagt cataaccatc
    26221 aaaatcatct aaaaaatgct ccgaccaatg agctaactca tgtcctacaa tctgtaagag
    26281 gaaattttca gataaagaat gatagtgact ttctattgct tgagtttgct gagaacactc
    26341 gtagtccatc aattgataca aatacaactc tttccaaaca gctaaatcag gcgccataac
    26401 cattcgtcta ttatttgtat aggctggaac agcgctttcc ctaataatct gtgtagcagc
    26461 attaaaatct gaccaaataa cggcttgcgg taaatcacag actgcaagtt cattctttaa
    26521 aaaagattga taatctttca acttttcagt atttcttact acaaaatcac gaaatgtagc
    26581 tagttgacta tcgtctttta caagataaag gttgtccacg gctctccttt ctttcacaca
    26641 atagactgaa taagcaaaag aaccgcgtcc gacaatccta ggttgattta acctaggggc
    26701 gtcagaaacg cggttccacc ctaatttatt acttcattgt ttttgaaaat actacagaaa
    26761 gcgccagctc attattttgc cctacttggc tcccactatc ccaagctcgc ttgaagaacg
    26821 ccataagact ttctttcctg ctgactatta tatcaaaaat tagaataatg tacaattctt
    26881 tttatgattt tctagaaatc cacatcatca actcgtggag cacccgattg aacagcaatc
    26941 gtttttaagt tttctctctc ctcatgactc agttcaattc caaagcaatc aagattagct
    27001 tgaatacgag aggttgtgac agattttgga agtggtaaaa atccttctgc caagctccag
    27061 gccaaggcta tctgagcaat cgattttccg tgatttgctg cgatttcttg cacttgcttg
    27121 ctatcaaata gttctccctg accaaaaggc ccccaagctt ccaataaaat tcccttttca
    27181 cgacagtaag ctacgacttg atcttgatac acacctggcg ccaagcgaac ttgattgacc
    27241 gcaggaacga tagttgcagt ttcaagcaag gcatccaaat gatggggaag aaaattgcta
    27301 acgccgatag cacggatttt cccttcttga tagaggtctt ccatcgctct ccaaacttcc
    27361 gcattgcgag ttttccatgc gtcattttct ctgagcggtt ttgggttcgg ccaatgaatc
    27421 aaatacaaat ccaaataatc caagcccagt ttttctatag atttttccaa agcttgacga
    27481 gtttgctcat aggtttgctg actattccaa agcttggtag ttacgaacat ttcttcacgt
    27541 ggaactccgc tatctttgat tgcttgacca acactttctt cattctgata aatcgccgcc
    27601 gtatcaatat gacgataacc agccttcaag gcttctaaca ctgcatgata ggcttcttct
    27661 ccatccttag ccttaaaagt tccaaatccc aatactggaa tttctactcc attatttaat
    27721 tgataggtat tcatatgctt ctccttctat tttattcatc aattatagca taagacattt
    27781 tttaagtagt actatcttct gtaaaaaata agtctgtcaa atatctaaca gacctatttt
    27841 ttcttgctta ttcatcacga ctatagtaca gctcatgagc ttttaggcga gtattgagca
    27901 tctctggtat ggtcacaaag gtataacctt gatttttcaa atgctcaatg acccttggca
    27961 aggcattgac tgtcggactg tgaatatcat gcatcaaaac gatagagccg ttagctactt
    28021 gatgctgaat ttctgtcaaa atagatgctt catttttact cttccagtcc agactatcca
    28081 catcccacat gataaagctc aaatccaagc tattgcgaat atcatctgta atagcaccat
    28141 aaggtggacg catgagttta gaactagaac ccagcacttt agttagcaca tcctcagtat
    28201 cagtaatctg ctttttagct tcatcaagag agagttgcga gagaatcgga tggctccaac
    28261 tatggtttcc aacaacatga ccttcagatt ttatcctctt caccaagtcc tcattcccag
    28321 aaacattttt cccaagcaca aagaaagtcg ctttaatatc atatttagct agggtctcta
    28381 atacctgcgg ggtcgttgct ggatttggac catcatcaaa ggttagagcg acaacttttt
    28441 gatatttctt atcaaagtaa gattggtaca aggccgcatc tttttcgagt aagtacgaag
    28501 attggataac atcaaagaaa gcagatactg gcaaggctat ctcttctaaa ttttcaagca
    28561 caggacttgg ataaaggata atctgactat ccttgtaatc aaaattccat gcagacaagt
    28621 cttggtcaga gaagtttttt acaatctgct cactctggtc ttgctctatt tttttatcct
    28681 ctatgaaggg ggtcaactct tttatcagct gttccttagc cttactagca tctgaaaaca
    28741 gttggtcaag tgtaaaaggt tgcccatctt ctgttaagtg aacctttcct agactggtct
    28801 tttcagtctc ttcaacctta aaagcaacta aatcatagat ttgtttggtc acattacgat
    28861 taacgactcc ctttaaacct gactcttctt gctctgtata gtagaaaaca agattgtcct
    28921 tactttctag cttgtcctta acatcttgat ttatcaactc cctaacagag gaaatcactt
    28981 tctccccttg gagaggataa taggcaatca cttcggcttg cccctgacga aaatgctcct
    29041 tctgatttcc ctcactcaat tgatcatctt tctctttttt gagcgattca atcttttgct
    29101 caaaactttt ttgctggtag atcttaaaag caataaatcc gcctaatagg caaatagaaa
    29161 ttgctaaaat accaatcaaa gccaataata catgtctcgt tttcccgtgt ctgccacgtc
    29221 ctagtctact tttattcata tttatatcat aacaaatttg agcaataatt tcaatgatac
    29281 aatttggcaa tccacaattc ctctagcaag ccaaagcatc ttacatcaac tccaacatcc
    29341 gtatctgtag agattatgta gttttctatc tatggaattt ctagtagatt gaagctagaa
    29401 tagtagacct atacttctaa aacattgtta gaaatcgatt tgattgtcct gatcgatttg
    29461 tcctgttctt atttcatttt actataaaac tatgagaagc taggtcatct gagaagtaat
    29521 tactatcgac aattgcgata gcgtatactg gtgcgatgtg tttgtagctc tggtgggtat
    29581 caccttcacg ttggcgaatt ttttctaggt tttgattaac ctgactgcac agataagccc
    29641 ataggcgatt gatgaaaaaa ttctgatgat gaacctgaat ttcgatgata acttggattc
    29701 cattatctaa ctccgccaag acgtctatac tagtatagaa atcctgcgct gagtagggca
    29761 tggaaggcaa gacgtgaatg ttacttccct ccaaaatcgt cacatttttt gctggcaagt
    29821 ccagcatatc gcggataaat tgacaagtga tttctggatt gctgaatatt tttttagcaa
    29881 ccaaatcatt agttgggctg atgcccggat gtctgagaat cctccttcta ttatagcaca
    29941 tttttaaatc taggtttgct agattctatg cttctatcta tttattcgga aaagaaagct
    30001 aatagcttat ttgaaatttt ctcctctctg ttggatagtc ccgtctatct atgttataat
    30061 gaaagaagga tttaaaatcg gaaaaggagt atttatgcta aaattaggtg tcatcggaac
    30121 tggcgctatc agccatcatt tcatagaagc agcccatacc agtggagaat accaactggt
    30181 cgcaatctat tctagaaaac tagaaactgc agcaaccttt gcttctcgct atcagaatat
    30241 ccaactattc gatcaattag aagtcttctt caaatcttct tttgatttag tctatatcgc
    30301 cagtccaaac tccttacatt ttgctcaggc aaaagctgcc ttgtctgctg gtaaacatgt
    30361 tattctcgaa aagccagctg tcactcaacc acaagaatgg tttgatttga ttcaaacagc
    30421 tgaaaaaaat aactgtttta tctttgaagc agctcgtaat taccacgaga aagcctttac
    30481 tactatcaaa aactttttag cagataagca agtgttagga gcagatttca actatgccaa
    30541 atactcttcc aagatgcctg acttgttgac tggacaaaca ccaaatgtct tttcaaaccg
    30601 ttttgctggt ggagccctca tggatttggg gatttatccc ctctatgctg ctgttcgtct
    30661 ttttggaaaa gccaatgacg cgacttacca tgctcaacag cttgacaata gcattgacct
    30721 aaatggagat ggtatcctct tctacccaga ctatcaagtt cacatcaagg ctggaaaaaa
    30781 catcacttcc aatcttcctt gcgagattta tacaacagat ggaaccttga ctctcaacac
    30841 gattgagcat attcgctcag ctatttttac cgaccaccaa ggaaatcaag tccagcttcc
    30901 tatccaacag gctcctcata cgatgactga ggaagtcgct gcatttgcac acatgatcca
    30961 gcaaccagat ctgaatctct accaaacttg gctgtatgat gcaggctctg ttcatgagct
    31021 actatatacc atgcgccaga ctgctggcat tagatttgag gcagaaaaat gaaaaccaaa
    31081 ctaccgactg aatggcaaga actgagtgac caactcggtt tccaagaatt cacccccatt
    31141 caaactcaac tatttgagcc ccttcttgct ggagaaaacc tcctaggagt gagccaaacc
    31201 ggaactggta agaccctagc gtatctccta ccaagtcttc tcagactaca aaagaaaaaa
    31261 gcccaacaac tcttgattct agcaccaaac acggaacttg ctggacagat ttttgatgta
    31321 tgtaaaacgt gggcagaagc tatcggattg actgctcaac tcttcctttc aggttcaagt
    31381 cagaaacgac aaattgaacg ccttaaaaag gggccagaaa ttctgattgg aactcctggc
    31441 cgtatctttg aactcattaa acttaaaaaa atcaagatga tgaatgtgga aaccatcatc
    31501 ctggatgaat ttgaccaatt actagatgat tctcagattc actttgtaga aaaaatcact
    31561 cactacgcac ctcgtgacca ccaactggtc tacatgagtg ccacgaccaa gtttgatcaa
    31621 gaaaagattg tgccgaatac acgcactatt gatctctctg atcaaaaatt ggacaatatc
    31681 cagcatttct acatgcaggt agaccaacgt catcgagtgg atatactacg aaaactggct
    31741 catgtagagg atttccgtgg tctagtcttc ttcaatagcc tgtcagacct tgggaatgcc
    31801 gaggaaaaac tacagtatcg tgatatattg gctgtttccc tcgctagtga tgttaatgtt
    31861 aaatttagaa aagtcatctt agaaaagttt aaagataacc aactaaccct gcttcttgca
    31921 actgaccttt tggctcgtgg aattgatatc gatagcctag aatgtgtcgt aaactttgat
    31981 attcctagag atagcgaaac ctacacacac cgtgctggcc gtactggccg tatgggcaag
    32041 gaaggatatg ttattactct cgtcactcat ccagaagaaa tcaaaaaact taaaaagttt
    32101 gcaagtatcc gagaaattgt cctaaaaaac caagaactct atatcaaata aagccccatt
    32161 gctctataca catccagtgt atggagcttt tttataagca attcaatttt atactcatct
    32221 gctttcaaaa agcattctat tccatctccg attaacgatg gactttatca cctccttctc
    32281 cagtccttgt atgacatctt ggagttgatt catgacatct tccaaagttc gaaaggcttt
    32341 attcttaaat ccacgtttac gaatctcttt ccacacttgt tcaatgggtt catctctggt
    32401 gtgtatggag gaataaaggt aaaatcaata ttagtcggaa tctttaaggt acttgattta
    32461 tgccatatag cattgtccat aacgagtaaa aaataatcat ctggataagc ttgtgaaagc
    32521 tcttctaaaa agacgttcat ccactcagta ttacatccac cagctattaa gaaaaatgat
    32581 tcgcctgtat ggggcatcaa cagctccata acaatagcga aattctcgta tatagtgact
    32641 atggacatgt ggacctactc ctattggaga ccaacaagat cccagtttac tgattctacc
    32701 gaaaccagcc tcatcttggt acatcaagcg aaccttatga aaaggtctac tggttttaaa
    32761 gcgctttctt gtcttcttga attgagattt tatttttaga cgcgacaatg gtttgagcgt
    32821 ctgctttctt aggatgttct ggacgtggca taatatttcg ccaaccatgg tgcttcaaca
    32881 gttgatagaa ggcatcacgt gtgtaggaac gatataagcc tgaaataagg tattgtaaca
    32941 aattctcctg cctctgtagc cttcaaatgg cgggcaagaa aggctttctc ttcctcaact
    33001 gtcatatatg catggttacg accaccacgt gtttcttgaa ggagagagtc gagtccgaac
    33061 tcctcatatt tttttacatt tcgccaaatc gttgtttgat tacagtctaa aagatctata
    33121 atctctttat aagatttgcc catcagacga aatagaacga tttgaaggcg tttatgatat
    33181 ttagctgtac gaaagtcttt taaaagtgtt ttgatggttt ggatttcttc ttcagttgat
    33241 ttcatattac tattgtataa tgctttttga tttgagtcta gtattatact cttcgaaaat
    33301 ctcttcaaac cgcgtcagct tcgccttgtc gtatagatgt tactgacttc gtcagttcta
    33361 tctacaacct aaaaacagtg ttttgagcag tctgcagcta actttctata acaaaatgaa
    33421 ataagaacaa gacaaatcaa tcaagacaat caaatcgatt tctaacaatg ttttagaagt
    33481 agatgtgtac tattctagtt tcaatatatt atagtttgct ctttgatttt cattgagtat
    33541 tagttcaata tctaaaaaaa gaggctctat aatatttgta gtgggtaaat cccctataga
    33601 tattatggag cctatttttg tgtagaaaaa aagtcccata tgacctataa tgaaaagcga
    33661 caaaacc
//
LOCUS       DAVKLX010000021        33165 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKLX010000021 DAVKLX010000000
VERSION     DAVKLX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33165
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.021"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            83..880
                     /locus_tag="VW325_001041"
     CDS             83..880
                     /locus_tag="VW325_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9734461.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1028..2287
                     /locus_tag="VW325_001042"
                     /pseudo
     CDS             1028..2287
                     /locus_tag="VW325_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2383..3657
                     /locus_tag="VW325_001043"
     CDS             2383..3657
                     /locus_tag="VW325_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9734462.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEHYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="VW325_001044"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="VW325_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9734463.1"
                     /translation="MNGIINLKKEAGITSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4806..5108
                     /locus_tag="VW325_001045"
     CDS             4806..5108
                     /locus_tag="VW325_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9734464.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5216..5854
                     /gene="udk"
                     /locus_tag="VW325_001046"
     CDS             5216..5854
                     /gene="udk"
                     /locus_tag="VW325_001046"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9734465.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5982..7322
                     /locus_tag="VW325_001047"
     CDS             5982..7322
                     /locus_tag="VW325_001047"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9734466.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7300..7512
                     /locus_tag="VW325_001048"
     CDS             7300..7512
                     /locus_tag="VW325_001048"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9734467.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7509..8384
                     /locus_tag="VW325_001049"
     CDS             7509..8384
                     /locus_tag="VW325_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9734468.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQIIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8377..9192
                     /locus_tag="VW325_001050"
     CDS             8377..9192
                     /locus_tag="VW325_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9734469.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQIFAEIKEAVERAHSQFKNE"
     gene            9185..9616
                     /locus_tag="VW325_001051"
     CDS             9185..9616
                     /locus_tag="VW325_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9734470.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9623..11290
                     /gene="recN"
                     /locus_tag="VW325_001052"
     CDS             9623..11290
                     /gene="recN"
                     /locus_tag="VW325_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9734471.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKERIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11292..12020
                     /locus_tag="VW325_001053"
     CDS             11292..12020
                     /locus_tag="VW325_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9734472.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12099..13922
                     /gene="lepA"
                     /locus_tag="VW325_001054"
     CDS             12099..13922
                     /gene="lepA"
                     /locus_tag="VW325_001054"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9734473.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(14216..14296)
                     /locus_tag="VW325_001055"
                     /pseudo
     CDS             complement(14216..14296)
                     /locus_tag="VW325_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            14470..14946
                     /locus_tag="VW325_001056"
                     /pseudo
     CDS             14470..14946
                     /locus_tag="VW325_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            14984..15286
                     /locus_tag="VW325_001057"
     CDS             14984..15286
                     /locus_tag="VW325_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9734474.1"
                     /translation="MIKILAACGAGVNSSYQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGSILFRIPAMSAPVFDNIILPAKQNMV"
     gene            15294..16097
                     /locus_tag="VW325_001058"
                     /pseudo
     CDS             15294..16097
                     /locus_tag="VW325_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            16146..16610
                     /locus_tag="VW325_001059"
     CDS             16146..16610
                     /locus_tag="VW325_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9734475.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            16767..17192
                     /gene="lacA"
                     /locus_tag="VW325_001060"
     CDS             16767..17192
                     /gene="lacA"
                     /locus_tag="VW325_001060"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9734476.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            17223..17738
                     /gene="lacB"
                     /locus_tag="VW325_001061"
     CDS             17223..17738
                     /gene="lacB"
                     /locus_tag="VW325_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9734477.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            17749..18678
                     /locus_tag="VW325_001062"
     CDS             17749..18678
                     /locus_tag="VW325_001062"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9734478.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLTKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            18680..19660
                     /gene="lacD"
                     /locus_tag="VW325_001063"
     CDS             18680..19660
                     /gene="lacD"
                     /locus_tag="VW325_001063"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9734479.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            19894..20730
                     /locus_tag="VW325_001064"
     CDS             19894..20730
                     /locus_tag="VW325_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9734480.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            20766..21083
                     /locus_tag="VW325_001065"
     CDS             20766..21083
                     /locus_tag="VW325_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9734481.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            21083..22774
                     /locus_tag="VW325_001066"
     CDS             21083..22774
                     /locus_tag="VW325_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9734482.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAKYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            22989..24395
                     /gene="lacG"
                     /locus_tag="VW325_001067"
     CDS             22989..24395
                     /gene="lacG"
                     /locus_tag="VW325_001067"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9734483.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSFNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGKKGSSKYQIKGVGRR
                     VAPDYVPRTNWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            24438..24695
                     /locus_tag="VW325_001068"
     CDS             24438..24695
                     /locus_tag="VW325_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9734484.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDDELEEFKNLFLNFINPEEWDTISFDSDFMPFQQS"
     gene            complement(24974..25735)
                     /locus_tag="VW325_001069"
     CDS             complement(24974..25735)
                     /locus_tag="VW325_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9734485.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(26023..26985)
                     /gene="nrdF"
                     /locus_tag="VW325_001070"
     CDS             complement(26023..26985)
                     /gene="nrdF"
                     /locus_tag="VW325_001070"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9734486.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(27173..29332)
                     /gene="nrdE"
                     /locus_tag="VW325_001071"
     CDS             complement(27173..29332)
                     /gene="nrdE"
                     /locus_tag="VW325_001071"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9734487.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(29413..29631)
                     /gene="nrdH"
                     /locus_tag="VW325_001072"
     CDS             complement(29413..29631)
                     /gene="nrdH"
                     /locus_tag="VW325_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9734488.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPEKLKQLA"
     gene            29982..30245
                     /locus_tag="VW325_001073"
     CDS             29982..30245
                     /locus_tag="VW325_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9734489.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            30251..31984
                     /gene="ptsP"
                     /locus_tag="VW325_001074"
     CDS             30251..31984
                     /gene="ptsP"
                     /locus_tag="VW325_001074"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9734490.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            32239..>33165
                     /gene="phtA"
                     /locus_tag="VW325_001075"
     CDS             32239..>33165
                     /gene="phtA"
                     /locus_tag="VW325_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU9734491.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGL"
BASE COUNT        10188 a         6016 c         7259 g         9702 t
ORIGIN      
        1 acttttatga aatatagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatggtttc ttcagaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg acacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat aatatggagg
     1021 tcaccttatg actcgtgatt ttaaatttga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atctgtttgc cttgaggaaa ccagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atacgatttt ggcgcttgcc catgctggtg agcatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttg tcgatgttga taatttggag gaagtagaag cagctatcaa
     1441 agacaatacc aagcttgtct tgattgaaac cttgggtaac cccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaaca ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgcgactaag tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg
     1681 tcgttttgac tggacggctt cagggaaatt ccctcaattt gttgacgaga gtccaagctg
     1741 ccacaatttg agctatactc atgatgtggg tgcagcagcc tttattatag ctgttcgagt
     1801 tcaattgctt cgtgatacag gtgcagcctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa accctttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag
     1981 tccttattat gccttggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt
     2041 ccacgtcaaa ggtgacgagg aagaagcacg caaggtcatt gataatttag aaatcttttc
     2101 tgaccttgca aacgcggcag atgttaaatc gcttgttgtc catccagcaa caatcactca
     2161 cggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaatc aaatccactt
     2221 gtctatcggt cttgaaaatg tagaagattt gattgaagac ttacgcttgg ccttggaaaa
     2281 aatttaaagt aaaagaagat aaacagtggg ttttgactca ctgtttttga ttttccctca
     2341 ggcatgatat aatggttaca gaagtctaga aagaggaatg taatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gaaagccagt atgctgaact tttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaggcagga actggccttg
     2521 gctgagcaaa aggctatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattgc agagtcaaat ccaaaacttt gatacagaaa aagaattggc caagaaagaa
     2641 gttgaacaga caagccatca ggccttattg gctaaggaca aggaagtaca ggccttggaa
     2701 aaccagttgg cgaccttgcg tttggagcat gaaaatcaat tgcaaaagac cctttctgac
     2761 ctagaaaaag aacgcaatca ggtcaaaaat cagctcctac tgcaagaaaa ggaaaatgag
     2821 ttgtctttgg cttctgttaa gcaaaactac gaagcccagc tcaaagcagc cagtgaacaa
     2881 gtcgagtttt ataagaattt caaagctcaa caatccacaa aagccatcgg ggaaagtttg
     2941 gaacactatg cggaaagtga gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa cgaagcggac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggctgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagtcacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc aattgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagatttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgact
     3541 acatctgaaa accaactccg tctagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat caacttaaaa aaagaagcag gaataacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat
     3781 ccggatgtgg tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggtcgagttt
     3841 atgcaggacg agggtaagat ctatgagggg gaaattactc tgggttattc cacgacgact
     3901 gaggatgcta gtggggaagt aatcgcagaa acccctgttt tgtctccctt ggatgaaaag
     3961 cttgttgatg aagcgattgc tagcttgact gggcctatta cccagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gaccatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgctcgatt cacttttcgt gtaaaatgca gtaaagggac ttacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact
     4261 cgtactagtg ctgctggctt acaattagaa gatgctcttg ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt
     4381 gtcaaagttt tcctaactcc agaagaggct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga actggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacggg gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa
     4561 aaatcgggtg atagataata attgcttgat aaaaccccat actaatagta gaatggtttt
     4621 gggaattata atattccaat tgttgcgagt tgtaggtact caaataatct attaggtact
     4681 caaataatct atatagaaat ttagaggtgt gaaatgaagc aatttaaaat tctttcagat
     4741 aaatatttag agtccattac aggttctggt gggaacttag gcccaggatt tggtgtgata
     4801 attccatgat gcgaaatgag tttcgagaaa gggtggagca acttcttcaa caaaaagaaa
     4861 taaatgaaaa tagtgagttg agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa
     4921 atgaaaaata ccaaacggtc atggccaatt tgagtcaagg gttgtcactt tacctcatga
     4981 cgcatcatta ccaggcacct aagtctgtca ttgattttgg tttatggatt gccaaagctc
     5041 ctagccagga aagagggaga ctggctttct tgcagatgct tgctcaaacc ctacagggct
     5101 ttcgttaaaa ctgtcgaaca tcagagggaa gactctggtg ttttttgctg atgagactat
     5161 aaattttgta aaaaatgtga tagaatagac gacggataaa ataacggagg atagcatgca
     5221 aaatagacca atcattattg gagtgacagg tggttctggt ggtggtaaaa ctagtgtttc
     5281 aagagccatt ttatcgcatt ttcctgatga aaagatttcc atgattgagc atgattcata
     5341 ctacaaggat cagtctcatt tgacctttga agagcgtgtc aaaaccaact atgaccatcc
     5401 ttttgccttt gatacagact tgatgatcga gcagattaag gaattgttgg cagggcgtcc
     5461 ggtggacatc ccgacctacg actatacaga gcatacacgg agtagcaaga cctatcgtca
     5521 ggagcctcaa gatgtcttta tcgttgaggg cattttggtc ttggaggaca agcgtctgcg
     5581 cgatttgatg gatatcaaga tttttgtgga tacggatgac gatgtgcgca ttattcgtcg
     5641 tatcaagcgt gatatggagg agcgtggccg tagccttgat agcgttatta accagtactt
     5701 aggtgtggtc aaaccaatgt accaccagtt tatcgagtca actaagcgtt atgctgatat
     5761 cgtcattcct gaaggggtta gcaataccgt ggctatcgac ctgttgacga ccaagattgc
     5821 aaagattttg gaagaagctc gaaacagcaa ataatcacat gtggaggcct tgcctccttt
     5881 ttctattttt ccctcataat ggtacataaa tggagatttt taggcatatt tttggtataa
     5941 taatacccat gaaagagcaa gtaaatgaat agtaggtgga gatggaaaag tatttatcgg
     6001 taacaacttt gaccaagtat ctgaaaatga aattcgataa agacccatac ttggaacggg
     6061 tctatttaac tggtcaagtt tccaactttc gtaaacgacc tactcaccaa tatttctccc
     6121 taaaggatga ccatgcagtt attcaagcga ccatctggtc tgggatttat cagaaattag
     6181 ggtttgacct ggaagaagga atgaagatca atgtgattgg gcgtgtacag gtctatgaac
     6241 caagtggtag ctactccatc atcattgaaa aagctgagcc tgatggggtt ggggcgcttg
     6301 cgattcagtt tgaacaactt aagaaaaaat tgacagaaga aggcctgttt caagaacgct
     6361 tcaagcaagc tctaccccaa ttttctaaga gaattggggt agtaaccagc cgtagtggag
     6421 ccgttattcg agatattatc acgaccgtca gcaggcgatt cccaggtgtt gacatccttc
     6481 tttatcctac caaggttcaa ggtgaagggg ctgcggagga aattgctaga aatattgcgc
     6541 gtgctaatca acgggacgat ttggatttgc tcattattgg tcgtggaggt ggttctattg
     6601 aggatctctg ggcctttaac gaagaaattg tggtacgagc tatttttgaa tctcgtttgc
     6661 cagttatctc tagtgtgggg catgagacgg atgtgacctt ggcagatttt gtggcagatc
     6721 gacgcgctgc aacgccaaca gcggcggctg aactggcaac acctgtgacc aagttggatg
     6781 tattaactca tttgcaaaat caggaaaaac ggatggcaac ggcagtccga aatgttctat
     6841 ctaagaaaca agaggctttg aaaaaatgca gtcagtctgt tatctttaga caacctgagc
     6901 gcttgtatga cggttatttg caacgcttgg accaactgca actgcgtttg aaacaaagtt
     6961 tgcgaactcg gatttctgat aacaaacaat tagttcaagc aagaactcat caattagtac
     7021 aattatcacc tgttaccaaa atccaacgct atcaagaccg tttaggacag ttggacaagc
     7081 tcttaggtag ccaaatggcg ttagtttatg acgccaaggt tgctgaggcc aagcgacttt
     7141 cggaagcttt gctcatgttg gatactagcc gaatcgtggc gcgtggttat gctattgtca
     7201 aaaaagaaga gtccattgta gattcggttg agagtttgaa gaaaaaagac caagtaacgc
     7261 ttttgatgcg agatggtcaa gtagaattag aggttaaaga tgtcaaaaca aaagaaattt
     7321 gaggaaaatc tagcagaact ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct
     7381 ctggaagatg cgattactgc ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct
     7441 acgctggaca aggctgaaaa gaccttggtc aaggtcatgc aagaagacgg aacagaaagt
     7501 gattttgaat gaaaaagcaa gaaaaattag ctcttgtcga gtcggctttg gaagattttt
     7561 atggagacca gcagtttgcc tctagtttac gggagtctgt tctctattct attcatgctg
     7621 gtggcaagcg tattcggcct tttctcttgt tagaagttct ggaagccttg caggttacca
     7681 tcaaacctgc tcacgcgcag gtagctactg ccttggagat gattcataca gggagcttga
     7741 ttcacgatga ccttcctgct atggatgatg acgattatcg aagagggcgg ttaaccaatc
     7801 acaagaaatt cggtgaagct atggccattt tggctggaga tgccttattc ttagacccat
     7861 atgccttgat tgcgcaggca gatttgccaa gtcagattaa ggtggacttg attgccaact
     7921 tatcccttgc ttcaggtagt ctgggtatgg tggcagggca agttttggat atggagggcg
     7981 aacaccagca cttgtctttg gaagaacttc agattattca tgccaataag actgggaagt
     8041 tactagccta tcccttccaa gcggcagcta ttatagctga attgtcacct gaaatgcagg
     8101 tgaagctgaa aactgtgggt gaattgattg gacttgcttt tcaagtcaga gatgatgtac
     8161 tggatgtgac agctagtttt gaggaaatcg gcaagacacc tcaaaaggat ctgcaggcag
     8221 aaaaatcaac ctatcctgcc ctgttgggct tggaagagtc cattgccttt tgtaaccaaa
     8281 ccctggatca agctaatgaa aaattagaag aaattgccca gcagcttccc tttgaaacag
     8341 aatcgattgt aagtgtagta gaaagtttga gaatcaatgg ctaaggaaag agtggatgta
     8401 ctagcttata aacaggggtt gtttgaaacg agagagcagg ccaagcgagg tgtaatggct
     8461 ggcctagtcg tagcagtcct taatggagaa cggtttgaca agccaggaga gaaaattcca
     8521 gatgacaccg aattaaaact caagggagag aaactcaagt atgtcagccg tggtggtttg
     8581 aaactggaaa aggccttgca ggtctttgat ttgtcggtgg atggcgcgac tacgattgat
     8641 atcggggcct ctactggagg ttttaccgat gtcatgctac agaatagtgc caagttggtc
     8701 tttgcagtcg atgttggtac caatcagttg gcttggaaat tacgccaaga cccacgagtt
     8761 gttagcatgg agcagttcaa tttccgctat gctgaaaaga ctgatttcga gcaggagccg
     8821 agctttgcca gtattgatgt gagtttcatt tcccttagtc tgattttgcc agccttgcat
     8881 cgtgtcttgg ctgatcaagg tcaggtggta gcacttgtca aacctcagtt tgaggcagga
     8941 cgtgagcaga ttgggaaaaa tggaattatt cgagatgcta agattcatca gaatgtcctt
     9001 gaatctgtaa cagctatggc agtagaggca ggtttttcag tccttggctt ggacttttct
     9061 cccatccaag gtggacatgg aaatattgaa tttttagcgt atttgaaaaa agaaaagtca
     9121 gcaagcaatc agatttttgc tgagattaaa gaagcagtag agagggcgca tagtcaattt
     9181 aaaaatgaat aaaaaagaga gacttgaaaa aattagaaga ttggtgacag attatcaaat
     9241 cggcacgcaa gaagaaattg tagaacattt gaaagaagca ggtatcactg ccactcaggc
     9301 gacggtatcc cgagatatca aagagttagg tattgtcaaa attcctttga gagacaacac
     9361 ctatgtctac gaattaccaa aatcaatcgt aaaaagtctg caactggctg aagacaatat
     9421 cgaatcggct gaattgatgg ataagatgat caatctccaa gttattccag gaaatacggc
     9481 ttttgtaaaa gctcagttaa tcgatacttt tgcagacaag atttttagtt gtttgactga
     9541 tgatagctcg attttagtca ttgccagaag tggaagtcta gcagaggaaa tctttgaaca
     9601 agtaaaaaat tggtaggtct atatgttact tgaaatttcg ataaaaaact ttgccattat
     9661 tgaggctatt tccctcaatt ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc
     9721 agggaagtcg attatcattg atgccatgaa tatgatgttg ggagctcgtg cgacgacaga
     9781 tgttattcgt catggtgcac caaaggcaga gattgagggg cttttctcag ttgagaatag
     9841 tcgtctttta caggaaattt ttgatgagca aggtttagaa ttaggtgatg aaattatcat
     9901 ccgtcgggaa atcttgcaaa atggtcgtag tatcagccgt gtaaatggtc agatggttaa
     9961 tctgtctgtt ttgcgagcta ttgggcaaca tcttgtagat attcatggtc agcatgacca
    10021 tgaggagtta atgcgtcccc aactgcatat ccagatgttg gatgaatttg gtgatgccgc
    10081 tttttgggac ttgaaagaaa cctatcaaac gagttttgat gcctatcgga aaatgcgcaa
    10141 gcaggttctg gaagtcaaga aaaaccaaca ggaacacaag gaacgtatcg aaatgttgga
    10201 atttcaaatg gcagagattg aggcagcaaa cttgcaggct ggagaagact tggctctcaa
    10261 tcaagagcga gataaactcc tcaaccacaa aaatattgcg gatacactga ccaatgccta
    10321 cagtatgttg gacaatgaag atttttcaag tctggccaat gttcgttcag ctatgaatga
    10381 catggaaagt gtcgaagagt atgatcctga ataccgtgaa atttcaagct ctctgtctga
    10441 gacctactat gttttagaag acattagcaa acgtttggaa gctatcattg aggaccttga
    10501 ttttgatggc aatcgcctga tgcaggttga gaatcgtttg gacctccttc atactattac
    10561 tcgtaagtat ggtgggactg ttgatgatgt tttgctttat tttgccaaga ttacggaaga
    10621 atacaatctc ttgacaggca ataatctttc gtctgaggac atggaagcag agcttaagaa
    10681 gttggaagtc aatcttgtca atttggcagg tcaacttgct tctgctcgtc ataatttggc
    10741 tcagcaactc gaagctgaga ttaaacaaga actgcaagat ctttatatgg aaaaagccca
    10801 gtttcaggtt cattttagta agggaaaatt cagtcgtggg ggaaatgaaa tggttgagtt
    10861 ttatatttca accaaccctg gagaagactt taaacccttg gttaaggttg cttctggagg
    10921 ggaattatct cgtctcatgt tagccattaa gtctgccttt tcacgtaaag aaggcaagac
    10981 tagcattgtc tttgatgagg tggatacggg agtttcaggt cgtgttgctc aagctattgc
    11041 tcagaaaatt cataaaattg gtcagcatgg tcaggttttg gctatctccc atttgccaca
    11101 agtaattgcg attgcagatt atcaattctt tattgagaag attagtaatg accattcaac
    11161 ggtttcgact gttcgtctct tgacagtcga agagcgagtg gaggaagttg ccaagatgtt
    11221 ggcaggtgat gatgtgacag aagcagccct gacgcaagcc agagaattgt tgagaaacag
    11281 ggagaaataa gatgacagat tattatgtaa ttggagatgt tcacggaaaa gctgggatgc
    11341 tggaagacct tcttaaaacc tgggatggtc agacccagtt gctctttcta ggtgatttga
    11401 ttgacagagg tgaggatagt caccgtgttc ttgaaatggt caaggacttg gtggacaatc
    11461 aaggggctat ctgtttgtct ggaaaccacg agtatatgtt tttgacttgg ctagatgacc
    11521 cagaagaaag ttatgaccat tatcgtcgca atggtggaga tacaaccatt aactctatcc
    11581 taggtcgtcc cttggatgca ccagttgatg gagtagaaga tgccaagcgg gttgccgctg
    11641 aagcgtcaga cttggtcgaa ttcattcgtc aaatgccatt tgtagtagag acagacaagt
    11701 atatatttgt tcacgcaggt attgatttga ctttggacga ctggcatgaa acaacagatt
    11761 ataaaaaagt atggcttaga aaaccattcc acggagctga aaatcatact ggaaaaacca
    11821 ttgtcttcgg tcatacaccg gtctatggtt tgttacagca agaacgcggt acatctgagc
    11881 tttggactac agaagatggc aagattggaa tggatggagg agctgtttat ggtggtgtcc
    11941 ttcacgggat tgtctttaca gaccaaggaa tgacagaaca ccactttatc gaaaatgacg
    12001 gctttgtcgc tgaagattag tactcctagc agggtatggt cttgtcaaaa tatcaaaaac
    12061 aatttataat aaatagatac cctgaaagga agagaatcat gaacttagaa gaattgaaaa
    12121 aacgacaggg gaagatccgt aacttctcta ttatcgccca tattgaccac gggaaatcaa
    12181 ctctagcaga ccgcattttg gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc
    12241 agcttttgga tagcatggat ctagagcggg aacgtggaat tactattaag ttgaatgcca
    12301 tcgagttgaa ttacactgca aaagatgggg aaacttatat tttccacttg attgacacac
    12361 cagggcacgt tgactttacc tatgaagttt cacgttcgct agctgcctgt gagggtgcta
    12421 ttttggtggt cgatgcggct caaggaattg aggctcaaac tcttgccaac gtttatctgg
    12481 ctttggataa tgatttggaa atcatgccta tcattaataa aattgacctg ccggctgcag
    12541 atccggagag cgtgcgtaca gagattgaag atgtgattgg tttggatgcc agtgaagcag
    12601 ttttggcttc tgccaaggct ggtattggga ttgaagaaat cctcgagcaa attgtagaaa
    12661 aagtaccagc accaacgggt gatgtgacgg cgccacttaa ggccttgatt ttcgactctg
    12721 tttacgatgc ttaccgtggg gttatcctcc aagtgcgtgt catggacgga gtggtcaaac
    12781 ctggtgataa gattcagctc atgagcaata gtaagacctt tgatgtggcc gaagtcggta
    12841 tttttacacc aaaagcggtt ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg
    12901 cggcgtctat caagacggtt caggatactc gtgtgggtga taccgttacc ttggcaacca
    12961 atcctgcggc agaaccatta catggttata agcagatgaa tcctatggtc tttgcgggtc
    13021 tctacccaat cgaatcaaac aagtacaatg acctgcgtga agcgcttgaa aaattgcaac
    13081 tgaatgatgc tagtcttcag tttgaaccag aaacatctca ggcacttgga tttggtttcc
    13141 gttgtggatt tcttggactt ctccatatgg atgttatcca ggaacgttta gagcgtgagt
    13201 tcaatattga tctcatcatg acagctccgt ctgttattta caaagttaat ttgaccgacg
    13261 gtgagtctat ggatgtgtct aacccatctg agtttccaga cccaactaag attgcgacca
    13321 ttgaagagcc ttatgtcaag gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa
    13381 tggagctagc tcagcgtaag cgtggggact ttgtgactat ggactatatt gatgataacc
    13441 gtgtcaatgt tatctatcaa attcctcttg ctgaaatcgt ctttgacttc tttgataaac
    13501 ttaagtcttc gacacgtggt tatgcaagtt ttgactacga attgtcagaa tatcgcccat
    13561 ctaagctagt gaaaatggat atccttctca acggagacaa ggtggatgcc ctcagcttta
    13621 tcgttcataa ggactttgct tatgaacgtg ggaaactcat cgttgataag ctcaagaaaa
    13681 tcatccctcg ccaacaattt gaggttccaa tccaagcggc tattggacac aagattgtcg
    13741 ctcgtactga tatcaaggcc cttcgtaaga acgtacttgc taaatgttat ggtggtgacg
    13801 tttctcgtaa gcgtaaactc cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca
    13861 tcggatcagt tgaagttccg caagaagcct tcctcagcgt cttgagtatg gatgaagaat
    13921 aagttatcag aaatgccttt ccttgttcac aggaaattta tataagaatc aatagtaaag
    13981 caggcttaga tggtctgctt ttttagtttt tatactcttc gaaaatctct tcaaaccacg
    14041 tcagcgtcgc cttaccgtat gtatggttac tgacttcgtc agtttcatct acaatctcaa
    14101 aaccatgttt tgagctgact tcgtcagttt catctacaac ctcaaaacca tgttttgagc
    14161 tgacttcgtc agtttcatct acaacctcaa aaccatgttt tgagctgact tcgtcagttt
    14221 catctacaac ctcaaaactg tgttttgagt aacctgcggc tagcttccta gtttgctctt
    14281 tgattttcat tgagtattag aacagaaaaa tgcttggagt atttgtttgt gtgtttattt
    14341 ttatataaca aactataaac acaacaaaaa tataaaaaaa gagacaaaaa agaacagaaa
    14401 gtaattaaca acgatttcat atagtgttat acttatagca taaagttaat ggaaagaagg
    14461 gaaaacctta tggtattaga ttatttcttt gacaaaaacc ttgtgttttg cttagaagcg
    14521 gataatcaag aataactctt tgatcaggta gcaagcttgt tggaggaacg agaaattgta
    14581 actccaactt atcgtgaagc tttgatcacg cgtgaaaagt catttccaac tggtttagat
    14641 atggaatttc taggaaagga cttgccaaat gtagcgattc ctcacacaga tattgttcat
    14701 aatctagctg aaaaagtggt ggttgttcga ttagaaaaac cagtaacttt tcacaatatg
    14761 atagctcctg ataaggaagt agaagtatcc ctactcttct ttatcattaa caattcaagt
    14821 tcaagtcaaa caaatattct ggctcagttg atggactttt tcacaggaaa tggacatctt
    14881 gaagatttat caaaaatttc cgaaccagaa aaactttatg cttacattgc tgaagcaatc
    14941 gcttaatctt gtctattaaa aaataaaatc ggaggaaatc caaatgatta aaattcttgc
    15001 tgcctgcggt gcaggtgtta actcaagtta ccaaattaaa agtgctctag aagaagaact
    15061 ttcaaaccgt ggttatgatg ttcactgtga tgcagtcatg gtgaaagatg taaacgaaga
    15121 ccttatgaaa ggttacgata tctttacacc aatcgctgca acagatcttg gttttgaacc
    15181 aggtatccca gttatcgaag ctgggtcaat cttattccgt ataccagcaa tgagcgctcc
    15241 agtatttgac aatattatac ttcctgcgaa acaaaatatg gtatagtagt tctatgaatg
    15301 atgaagcaag taaacaacta actgatgcac gatttaagcg tcttgttggt gttcagcgta
    15361 ccacttttga agagatgtta gctgtattaa aaacagctta tcaacttaaa cacgcaaaag
    15421 gtggacgaaa acctaaatta agcctagaag accttcttat gcccactctt caatagtgcg
    15481 agaataccgc acttatgaag aaattgcggc tgattttggt attcacgaaa gcaactttat
    15541 ccgtcggagc caatgggttg aaataactct tgttcaaagt ggttttacgg tttcaagaac
    15601 tcctctcagt tctgaggaca cggtaatgat tgatgcgacg gaagtaaaaa tcaatcgccc
    15661 taaaaaaaca attagcgaat gattctggta aaaagaaatt tcacgctatg aaggctcaag
    15721 agattgtcac aagtcaaggg agaattgttt ctttggatat cactgtgaac tattgtcatg
    15781 atatgaagtt gttcaaaatg agtcgcagaa atatcggaca agctggtaaa atcttggctg
    15841 acagtggtta tcaagggctc atgaagatat atcctcaagc acaaactcca cgtaaatcca
    15901 gcaaactcaa gccgctaata gctgaagata aagcctataa ccatgcgcta tctaaggaga
    15961 gaagcaaagt tgagaacatc tttgccaaag taaaaacgtt taaaatgttt tcaacaacct
    16021 atcgaaatca tcgtaaacgc ttcggattac gaatgaattt gattgctggt attatcaatc
    16081 ataaactaga attctagttt tgcaggaagt ctaataaaga aactagcaag gtcaggcctt
    16141 actttattgg tggacatcca ggatttaatt gtcttctact tgatgatgaa gtctatgaag
    16201 attattatct agagtttgag aaagaagaga tttgctctgt tccacgtcct ttcccagaaa
    16261 caggtatgtt ggatttccaa gatagaagtc catggctaga gggacaaaaa gaaatagatc
    16321 ttagttatga tcttttcagc acagatgcag tgactttgga tgaattgcaa tctcgaacaa
    16381 ttgcccttcg ttctcttaaa catgataagg gattgaaagt gcattttgca gaatttccaa
    16441 acctcatcat ctggtcaact ttgaacaagg gacctttcat tacctttgaa ccatggtctg
    16501 gcttgtcaac attccttgaa gaaggagatc atttagaaga taagaagaat gtttgtctct
    16561 tagaagccaa tcaggttgaa gaattagggt ttgagataga agttttataa aaaagcaaaa
    16621 aataaataac aaaaacaaac acaaactgtt gacttcttca aacaatagta gtatattatg
    16681 tttgtgagga ctaattgact gttttttaaa gacagccaat acactgctgg aatccagcat
    16741 agaaaaaata aaaaggagta tacaatatgt ctattgttat cggtgcagat gctgcaggtt
    16801 tgagattgaa agaagttgtg aaagacttct tagaaaaaga aaacttccac cttgtggatg
    16861 ttacagctga aggtcaagac tttgttgatg tgactctcgc tgttgctgca gaagtaaaca
    16921 aagaagaaca aaaccttggt atcgtgattg atgcttatgg agctggtcca tttatggttg
    16981 caactaagat caaaggaatg gttgctgcag aagtatctga cgaacgttca gcttatatga
    17041 ctcgtggcca caacaactca cgtatgatca ctatgggggc acaacttgtt ggtgatgaat
    17101 tggctaaaaa tatcgctaaa ggatttgtta acggtaaata cgacggtggt cgtcaccaaa
    17161 ttagggtcga catgttgaac aaaatgggct aattagatta tagtaagaaa ggtaagttaa
    17221 aaatgagaat tgcaattgga tgtgaccaca tcgtaacaga tgaaaaaatg gcggtttcag
    17281 aatttttgaa atcaaaagga tatgaagtca ttgactttgg tacctatgac catacacgta
    17341 ctcactaccc aatctttggt aaaaaagtag gggaagcagt aactagtggt caagctgatc
    17401 tcggggtatg tatctgtggt actggtgttg gtatcaacaa cgctgtaaat aaagttccag
    17461 gtattcgttc tgccttggtt cgtgatatga caacagccct ttatgctaaa gaacaattga
    17521 acgccaacgt tatcggtttt ggtggtaaga ttactggtga attgcttatg tgtgatatca
    17581 tcgaagcttt cattcatgct gaatacaaac catctgaaga aaacaaaaaa ttgattgcga
    17641 aaattgaaca ccttgaaagt cacaatgctc aacaaacaga cgcaaacttc ttcactgaat
    17701 tccttgaaaa atgggatcgc ggagaatacc acgactaaga ggtaacctat gattttaaca
    17761 gttacaatga acccatccat tgatatttcc tatcccttgg atgagttgaa gattgatact
    17821 gtcaatcgtg tggtggatgt aaccaaaacg gctggtggta agggactcaa tgttacccga
    17881 gtactttcag aatttggcga ttctgttctt gctactggtt tagtgggtgg caaacttggt
    17941 gagtttttgg ttgaacatat cgataatcaa gtaaagaaag atttcttctc aattcaggga
    18001 gaaactcgta actgtatcgc tattctccac ggagacaacc aaacagaagt tcttgaaaaa
    18061 ggtcctgaag tattggaaca ggaaggtcaa gactttttgg aacatttcaa aaaactcttg
    18121 gagtcagttg aagtagtggc catctcaggt agtctgccag ctggccttcc agttgattac
    18181 tatgcgagct tggtagaact tgctaatcaa gctggcaagc ctgtagtctt ggactgctca
    18241 ggtgcagcac ttcaggctgt tcttgaatca ccccataaac caacagtcat caaaccaaat
    18301 aatgaagaat tgtctcagct tcttggaaga gaagtttctg aggatttgga tgaattaaaa
    18361 gaagtacttc aagaaccttt gtttgcaggg attgaatgga ttatcgtttc acttggtgcc
    18421 aacggtactt ttgccaaaca tggtgacact ttctacaagg tagatattcc tagaattcag
    18481 gtggtaaatc ctgttggatc tggagactct actgtggcag gaatttcttc aggacttctt
    18541 cacaaagaat cggatgcaga attactcacc aaggcaaatg tccttggtat gctcaatgct
    18601 caagaaaaaa tgactggtca tgtcaacatg gccaactatc aagttctata tgatcaatta
    18661 atagtaaaag aggtataaaa tggctttaac agaacaaaaa cgtgtacgct tagaaaaact
    18721 ttctgatgaa aatggtatca tctcagctct tgcatttgac caacgtggtg ctttgaaacg
    18781 cctcatggct caacaccaaa cagaagaacc aactgtggcc caaatggaag aacttaaagt
    18841 cttggtagca gatgaattga ctaaatacgc ttcatctatg cttcttgacc ctgagtatgg
    18901 acttccagca actaaagctc ttgatgaaaa agctggtctt ctccttgctt atgaaaaaac
    18961 aggttatgac acaacaagca caaaacgctt gccagactgc ttggatgtct ggtctgcaaa
    19021 acgtattaaa gaagaaggtg cagatgcagt taaattcttg ctttactatg atgtagatag
    19081 ctcagacgaa ctcaatcaag aaaaacaagc ctacatcgaa cgcatcggtt ctgagtgtgt
    19141 ggctgaagat atcccattct tccttgaaat tcttgcttac gatgaaaaaa ttgcggatgc
    19201 aggttctgta gaatacgcga aagtaaaacc acacaaagtt atcggcgcta tgaaagtctt
    19261 ttcagaccca cgctttaaca ttgatgtctt gaaagttgaa gttcctgtta acattaaata
    19321 tgttgaaggc ttcgctgaag gtgaagtagt ttatacacgt gaagaagcag cagccttctt
    19381 caaagcgcaa gatgaagcaa cgaacttgcc atacatctac ttgagtgctg gtgtatcagc
    19441 taaactcttc caagatactc ttgtatttgc tcatgaatca ggtgcaaact ttaacggagt
    19501 tctttgtggc cgtgctacat gggcagggtc agttgaagct tacatcaaag atggtgaagc
    19561 agcagctcgc gaatggcttc gcacaactgg atttgaaaac attgatgagc tcaataaagt
    19621 tcttcaaaca acagcgactt catggaaaga acgtgtgtaa gaaagtcctc ctagtttagg
    19681 aacatgaatc taaaaaaatt taaaaaaagt tgtatgtaaa ggtttacaaa ataacttact
    19741 tgtgctatac ttaaatcaca agttaataca aggtgagtgt tactaagtaa tattaggcat
    19801 gatcacaggt gaattagaaa tcagctgatt ttctagttca tttgtggtca ttttttgtac
    19861 ttatatacct ttaagatata aaaggaggtt gacatgtatc gaattctaaa tccaatgaat
    19921 cacaatgtct cgcttgtcag aaatgataag ggagaagagg tgattgtaat tggtaagggg
    19981 attgcattcg gaaagaagaa gggggatttg attgctgaaa atcaggttga gaaaatcttt
    20041 cggatgaaga ccgaagagtc cagagaaaac tttatggctc ttctcaaaga tgttccgctt
    20101 gattttatca cagtgaccta tgaaatcatt gataagctat caaagaaata tcattatccg
    20161 attcaagagt atctctatgt aaccttgaca gatcatattt actgttctta tcaagctcta
    20221 actcaaggaa ggtacaagga tagtaatctg ccagatattt ccgctaagta tcctgtcgct
    20281 tttcaaatcg caaatgaagc ttttgaaatt taccgtcaga agctagcaga tcattttcct
    20341 gaggacgaaa ttattcggat tgcttatcat ttcattaatg ctgaaggtga aaatgaagtg
    20401 gaacttgtgg agtcgattga taagaggaaa gaaattctca ggaatgttga agaagtttta
    20461 acggactatg caattcaacg aactaaaaag aataaccatt tctatgatcg ctttatgatc
    20521 catttgaatt atttcttgga ttatttagac agatctagag atgataacca atcacttctg
    20581 gatatggaag atcatattaa acaatcctat ccaaaagcct tcgagattgg ttccaagatc
    20641 tatgatgtga ttacgcaaca tacgggtctt gatttgtata aaagtgaacg agtttatcta
    20701 gttctacata tccaacgttt attgtcataa aaatttattt aaaactatat aaggagaatt
    20761 ctatcatgaa tagagaagaa gtaacattgt taggttttga aatcgtagcc tatgctggcg
    20821 atgctcgttc aaaactattg gaagccttga aggctgctga agctggtgat tttgaaaaag
    20881 cggacgctct ggtagaggaa gctggtagct gtattgcaga ggctcaccac gcgcaaacaa
    20941 gtctattgac taaggaagct tcaggtgagg acttggctta tagtgtaacc atgatgcatg
    21001 gccaagacca cttaatgaca actatcttgt taaaagattt gatgcatcat ttaattgaac
    21061 tctacaagag aggagttaaa taatgaataa actaattgca tttatcgaga aaggaaagcc
    21121 tttctttgaa aaactatctc gtaatatcta tcttcgtgct attcgtgatg gtttcattgc
    21181 aggtatgcct gttattctct tctcaagtat ctttatcttg attgcctttg taccaaactc
    21241 atggggcttt aaatggtctg atgaagttgt agcctttctg atgaaacctt atagctattc
    21301 tatgggtatt ctggctctct tggtagctgg tacaacagct aagtcattga ctgactcagt
    21361 aaaccgtagc atggaaaaaa ccaatcaaat caactatatg tcaacattgt tggcagcaat
    21421 tgttggtttg ttgatgttgg cagctgatcc tatcgaaagt ggtctagcta ctggattctt
    21481 ggggacaaaa ggtttgcttt cagccttcct tgctgccttc gttactgtag ccatctataa
    21541 ggtttgtgtt aagaacaacg tcactattcg tatgcctgac gaagttccac caaatatctc
    21601 acaagtcttt aaagatgtga ttccattcac tctctcagtt gtttcacttt atgctcttga
    21661 tttactagct cgtcattttg ttggtgcaag cgtagctgaa tcaatcggta aattctttgc
    21721 accactcttc tcagcagcag acggatacct tggtattacc attatctttg gtgcctttgc
    21781 cttcttctgg tttgtgggta ttcatggtcc atctattgtt gaacctgcta tcgcagcaat
    21841 tacctatgca aatgctgaag tcaacttgaa ccttctccaa caaggtatgc acgctgacaa
    21901 gattcttact tctggtacac aaatgtttat cgttaccatg ggtggtactg gtgcgacact
    21961 ggttgttcca ttcatgttca tgtggttaac aaaatcaaaa cgtaaccgtg caatcggacg
    22021 tgcttcagta gttcctacct tcttcggtgt aaatgagcca atcttgtttg gtgcacctct
    22081 tgttttgaat ccaatcttct tcattccatt tatctttgct ccaattgcaa acgtatggat
    22141 ctttaaattc tttattgaaa ctcttggaat gaactcattc actgctaatc taccatggac
    22201 aaccccagct ccactaggtc tagttcttgg tactaacttc caagttctat cattcattct
    22261 tgctgccctt ctaatcgtgg ttgacgttgt catttactat ccattcctta aggtctatga
    22321 tgaacaaatt cttgaagaag aacgttcagg taagtctaat gatgaattga aagaaaaagt
    22381 tgctgcaaac ttcaacactg caaaagcgga tgctattctt gaaaaagcag gtgtagaagc
    22441 agcacaaaat acaatcacta aagaaacaaa tgtcctcgtt ctttgtgcag gtggaggaac
    22501 aagtggtctc cttgcaaatg ctttgaataa ggcagcagca aaatacaatg tccctgtgaa
    22561 agcagcagca ggtggctatg gtgctcaccg tgaaatgttg ccagagtttg atcttgttat
    22621 ccttgcccct caagttgctt caaactttga agatatgaag gcagaaacag ataagctcgg
    22681 tattaaactt gctaagacag aaggcgctca atacatcaaa ttaactcgtg atggaaaagg
    22741 tgccctagca ttcgtacaag agcaattcga ttaaggctag gaactgtgaa acagtctccc
    22801 atcgttacgg aaatcgctat ggcgaatttc ctaatcgcct tattaattcg tcggtaaaaa
    22861 gatatcgttt ttacctcctc atgtcacaat tcagtgactt ggcacaagaa gtgagatgga
    22921 gaagcatggc tcactgactc ctctcctctc acttttactt tatttaaatc aagaaatagg
    22981 tgaaaaaaat gacaaaaaca cttccaaaag actttatttt cggtggcgca acagctgctt
    23041 atcaagcaga aggtgctaca catactgatg gaaaaggacc agttgcttgg gataaatatc
    23101 ttgaggataa ctactggtac actgccgaac cagctagtga tttttacaat cgatatccag
    23161 ttgacctcaa gctagcagaa gagtatggtg tcaatggtat tcgaatttct attgcttggt
    23221 cacgtatttt cccgactggt tacggccaag taaatgctaa aggtgttgag ttttatcata
    23281 atttatttgc agagtgtcac aaacgtcatg ttgagccctt tgtaactctt catcactttg
    23341 acactccaga agctctccac tcaaatggag acttcttaaa ccgtgaaaat atcgaacatt
    23401 ttgtagacta cgctgccttc tgttttgaag aatttccaga agtaaactat tggacaacct
    23461 ttaatgaaat tggaccaatc ggtgatggtc aatatttggt tgggaaattc cctccaggta
    23521 tccagtacga ccttgccaaa gtctttcaat cacaccacaa tatgatggtg tctcatgcac
    23581 gcgcggtaaa attgtacaaa gagaaaggct ataaagggga aattggtgtt gttcacgccc
    23641 tgcctactaa atatcctcta gatcctgaaa atccagcaga tgttcgtgca gctgagttgg
    23701 aagatatcat ccacaataaa ttcatcttag acgcaactta tctaggtcgc tattcagctg
    23761 aaaccatgga aggtgtcaac catatcttat cattcaatgg tggtagtttg gatcttcgtg
    23821 aagaagattt tacagcatta gaagctgcaa aagacttgaa tgatttccta ggaatcaact
    23881 actatatgag tgactggatg gaagcctttg atggagaaac tgaaattatc cataatggta
    23941 aaggtaaaaa aggaagctct aagtatcaaa tcaaaggtgt tggtcgtcgt gtagctcctg
    24001 actatgtacc acgcacgaat tgggattgga ttatctaccc tcaaggtttg tatgaccaaa
    24061 tcatgcgtgt gaagaaagat tatcctaact acaagaagat ttacatcact gaaaatggtc
    24121 tcggctataa agatgagttc gttgataaca ctgtttacga tgatggtcgt attgattacg
    24181 tgaaacaaca cttggagatc ttgtctgatg cgattgcaga tggagctaat gtaaaaggtt
    24241 acttcatttg gtcattaatg gatgtcttct catggtcaaa cggttatgag aaacgttatg
    24301 gtctcttcta cgtagatttt gaaactcaag aacgttatcc taagaaatca gctcactggt
    24361 acaagaaagt agcggaaact cagattatag actagtagaa ttagtcatta gatatagaat
    24421 tttagtgagt caaaaagatg ttcaaagatt ttatccaatc tatttatgaa aaagtttata
    24481 ttataaattt cgaaaaatgc tctcaaatac cgtgtttgac gagtgaagaa ttgaaaagtc
    24541 ttggaaaatg gtatgtctcg actggtaaag aatggatttg tcattcagat gatgagctgg
    24601 aagaatttaa aaatctattt ttaaatttta tcaatcctga agaatgggat actatctcct
    24661 ttgattcaga ttttatgccg tttcaacaat cgtaaccaat ttctcaaaaa agttaaatct
    24721 tatatttagt actctgtaaa actcttatct aatcacgtta cttatactca atgaaaatca
    24781 aagagcaact ttaaactagg aagcgagtcg cagatttctc aatgtatagc tttgaggaat
    24841 tgggcaaaaa gtctttgata tagaaaaaag catagtatca ggtgttgaaa cacctgatac
    24901 tatgcttttt attgtgggaa gatttacttt ttttcttctg aaattgagtt gttacccagg
    24961 ctctttcagt ttattaaggc ttgatgactt taatgtgttt agatagctta aaaaggattg
    25021 aatcacttag tttagaatct gaaacaatag tatcaagatt tgatacatta taaaaagtat
    25081 aaaaatcaaa cttattgaac ttactatgat ctgcgagtaa atatttttta ttagaattat
    25141 ttaaagcgat gcgttgagcc tctccctctt cctcgctaaa agtagctaga gctccgtttt
    25201 gaataccatt acagctaacg aaagctttag aaaattggag attagagaga ttttgtaggg
    25261 tcaatgtacc aacaaaagca cctgtaatat cgcgataatt tccacctatt aaaatcaaat
    25321 ctgttaattt tcgttcgctt aaaatcagaa aaacaggtag actgttggtt acgacgcgga
    25381 tattgtcaat aggcaactca cgcgcaaaaa actctaatgt tgttcctggt ccaatgaaaa
    25441 tagtttctct ttcttctact agactgcctg caaaatgggc tatttcttgt ttttctgccg
    25501 tttggagggc ttgtttttca atatttgatc gctcattagt caaaagggag ttggttcgaa
    25561 gtttttcagc tccaccatgc acacgaatca gcaaatcttt atcagctaat tcctgtaaat
    25621 agcgccttgc agtcatatct gaaacggcta tttcgtccat aatctgttta actgttatgg
    25681 ttcctttact atttactatc tctaaaattt tggttaattt ttcttgtttg agcatattat
    25741 cacctcgttt cctactacta tcttaccata aacaaactca tcattcaaat acaaaaacaa
    25801 caaaatgaaa caaaaacaaa aatatcgaag tttgttttca aaactttcga tatttttgtt
    25861 gggttattat tttgttgttt ctagtttact ttttgatggt taagagtggt ggagaattat
    25921 actcaatgaa aatcaaagag caaactagga agctagccgc aggctgtact tgagtacggc
    25981 aaggcgaagc tgacgtggtt tgaatttgat tttcgaagag tattagtcca aaccgtagtt
    26041 gtagtcatca tcttgcatgg cttcaacttc gccaagaagg taaccatttc cgacttgaga
    26101 gaagaagtca tggttggaag ttcctgttga aataccgttc ataacgattg ggttgacatc
    26161 ttcagctgaa tctgggaaaa gtggatcttg tcccatgttc atgagagctt tattggcatt
    26221 gtagcgaagg aaggttttaa cctcttcagt ccaaccaaca ccgtcataaa gactctctgt
    26281 gtagccttct tcattttcat aaagagtata gagtaggtcg tacatccatt ctttgagttt
    26341 ttcttgctct tcttcaggca attcattgaa accaagttgg aatttgtaac caatgtaggt
    26401 tccgtgaaca gactcgtcac gaataatcaa tttaatgatt tctgcaacgt tggctagttt
    26461 gttgttaccg agatagtaga ggggagtgaa gaaaccagag tagaagagga aggtttcgag
    26521 gaagacgctg gcaactttct tttcaagtgg gctgccgttt aggtagattt cgttgacaat
    26581 ctcagccttc ttttgtaggt aaggattggt attggtccat tcgaaaattt cttcaatctc
    26641 agccttagta ttcaaggtag aaaagattga tgagtaagat ttagcgtgga cagattccat
    26701 aaattggatg ttattgaaaa cagcttcctc atgtggtgta cggatgtctg cgcgaagggc
    26761 ttgaacccca gtttcagatt gcatagtgtc aagaagggtt aaaccaccaa aaacttttcc
    26821 gaccaagtct ttctctttgt tagatagctt tctccagtca tccaagtcgt ttgataaggg
    26881 aatacgtgta tcgagccaaa attgctccgt cagtttttcc caagttgatt tgtcgatgac
    26941 atcttcgatg gcattccagt taatggcttt gtagtaagtt tccatttaaa atctctttct
    27001 gtgtttagta ttgcgaactc acaattattt ctactttacc ataattctat aggagtatcg
    27061 cacaaaaagt cggaagcccg acttttaaaa tgttacataa attatgttat gacatagtag
    27121 atttgatttt atcagtgctg cttagggaaa aataatgttt ctatgctaga aactaaatca
    27181 cacagctttc acattggttg gcgccgactt ctccaccgtc atctgtaaag gtacggacgt
    27241 agtagataga cttgattccc ttgttaaagg catagttacg aaggatggac aagtcacgtg
    27301 tcgtttgttt attttccctc ttccattcgt aaaggccttt tggaatgtca ctgcgcatga
    27361 agagggtgag tgaaagtcct tgatccacgt gttcagtcgc agcagcgtaa acatcgatga
    27421 ctttacgcat atccatatcg taggcagaag tgtagtaagg aatggtttct gtagacaagc
    27481 cagcagcagg gtagtagatt ttaccaattt tcttctcttg gcgttcttcg atacgttgcg
    27541 taatcgggtg gatagaagca gaaacgtcgt tgatatagct gatagaacca tttggcgcta
    27601 cagcaaggcg attttggtgg taaagaccat cttcttgaac cttgtcgcga agttcagccc
    27661 aatcagcaac accagggata aagacatttt tgaagagttc tttaacacgg tctgatgttg
    27721 gaacaaattc accagttaca tacttgtcaa agtaacttcc gttagcatag tctgattttt
    27781 caaagttgtg gaaggtaata ccacgttcac gtgcaatatt gtttgactct accaaggtcc
    27841 agtagttcat aagcataaag tagatgcttg taaattcaac agactcaggt gaaccatatt
    27901 caatgagttg ttgggcaagg tagctgtgca gtcccatggc accgagacca aaggtgtggg
    27961 cttggctatt tccatggtca atcgttggta cagctacgat atgtgaacta tctgtaacga
    28021 aagtaagggc acgaaccata gcacggatag aacgaccaaa atcaggtgaa gtcatcatgt
    28081 taaccacgtt ggttgaaccc aggttacatg aaacatctgt tcccatttga aggaattctt
    28141 gagcatcgtt gatcaagctt ggttcttgaa cttgaagaat ctcagaacac aagttactca
    28201 tgataatctt tccatcaaca ggatttgcac ggttagccgt atcgatgttg actacataag
    28261 gatagccaga ctcttgttgc aatttagaga tttcagtttc caaatcccgc gccttgattt
    28321 ttgtcttgcg aatatttgga tttgcgacca attcatcgta tttttcagta atgtcgatgt
    28381 aattgaatgg cacaccgtat tctttttcta cagagtaagg gctgaagagg tacatttctt
    28441 catttttacg agccaattcg tagaatttat caggtactac aacaccaagt gatagagtct
    28501 tgacacgtac tttttcatca gcgttttctt tcttagttga aaggaaagcg atgatatctg
    28561 ggtgaaagac gttgaggtag acaacaccgg caccttgacg ttgccccaat tggttggagt
    28621 aagagaagct gtcttcaaaa agcttcataa caggaacgac acctgaagca gctccttcat
    28681 agcctttgat aggtgcacca gcttcacgaa ggttgctgag ggtaattccc acaccaccac
    28741 caatacgtga aagttgaaga gctgagttga tagaacgtcc gatagagttc atatcatccg
    28801 tcacttggat taggaaacaa gataccaact ccccacgacg agcacgtcca gcattcaaga
    28861 aggaaggagt agcaggttgg tagcgttggt ggatgatttc attggcaata tcgattgcaa
    28921 cagcttcatt cccatcagcg aaataaaggg cgttgaagaa gacacggtct tccatatttt
    28981 caagatagta ttcaccgtca ttagtcttta aggcatattg attgtaaaat ttataagctg
    29041 ccatgaatga cttgaattgg aagttttggt ctttgataaa ttgagccaat tcttccaaga
    29101 actctggacg gtatttcttg ataaaggctg tttcgatgta gttgtgttca atgaggtaat
    29161 tgattttgtc tttgattgaa tcaaaaacca tagtgtttgg aactacattt tctttaaaga
    29221 aagcatccaa ggcttcctta tctttatgaa gcatgatttg tccattaaca ggacggttaa
    29281 tttcgttatt aagacggaag taagtcacgt cttcaagatg ttttaatccc ataaaatttc
    29341 ccttatctaa ttacaaaaga aaggcttcta agttagccct aaaagcagtt tcttctggat
    29401 gatgtactaa gattatgcta attgtttcag tttttctggt tggaaacctg aaaagacttc
    29461 agttggtgtt tggataacag gagctgcgct aaaaccgagc tctttaactt gatcgacgta
    29521 ctcaggttgc tcatcaagat tgatttcacg ataagagaca ttattactgt ccaagaaacg
    29581 cttggtcatt ttacattgga cacaattgtt tttagaataa acggttacca ttgtgtaact
    29641 cctcttcaaa atttaatact atcttagtat atcagaaaat aaaattttgt caatgattta
    29701 aactaaatat agtggtgttc ttttgttgtt tttatatata aacacaatat atagtgtttg
    29761 tattgtgaaa tagcaatgat tatcctataa attgtttttg aagaaactat atcctgtgtt
    29821 ttgttatcta ggatagaaga ttttcagcaa gttatctgaa aggaaattga aggaaaatcc
    29881 agaaaatgct tgaaaaaaat cctagaagat ggtataatac taaattgtaa gggttatcac
    29941 atataactca aaaaaagaaa gaacaaaagg agagtcaaac tatggcttct aaagatttcc
    30001 acgtagtggc agaaacaggt attcacgcac gtccagcaac attgttggta caaactgcta
    30061 gcaaatttgc ttcagatatc actcttgagt acaaaggtaa atcagttaac cttaaatcaa
    30121 ttatgggtgt tatgagtctt ggtgttggtc aaggtgctga cgtaactatc tcagctgaag
    30181 gtgcagatgc agatgacgct atcgctgcaa tctcagaaac aatggaaaaa gaaggattgg
    30241 cataagggaa atgacagaaa tgcttaaagg aatcgcagca tctgacggtg ttgcagttgc
    30301 aaaagcatat ctactcgttc agccggattt gtcatttgag actattacag tcgaagatac
    30361 aaacgcagaa gaagctcgcc ttgatgccgc tctacaggca tcacaagacg agctttctgt
    30421 tattcgcgag aaagcagtag gtacgctcgg tgaagaagca gctcaagttt ttgatgctca
    30481 cttaatggtt cttgctgacc cagaaatgat cagccaaatc aaggaaacta tccgtgcgaa
    30541 gaaagtgaat gcagaagcag gtctgaaaga agttacagat atgtttatca ctatctttga
    30601 aggcatggaa gacaacccat acatgcaaga acgcgcagcg gatatccgcg acgtgacaaa
    30661 acgtgtattg gcaaaccttc ttggtaaaaa attgccaaac ccagcttcta tcaatgaaga
    30721 agtgattgtg attgcgcatg acttgactcc ttcagataca gctcaattgg acaaaaactt
    30781 tgtaaaagct tttgtaacca acattggtgg acgtacaagc cactcagcta tcatggcacg
    30841 tacacttgaa attgctgctg tattaggtac aaacaacatc actgaaatcg ttaaagacgg
    30901 tgacatcctt gctgttaacg ggatcactgg agaagtgatt atcaacccga cagatgaaca
    30961 agcggcagaa tttaaagcag ctggtgaagc ctatgcgaaa caaaaagctg aatgggcact
    31021 tttgaaagat gctcaaacag tgactgctga cggtaaacac ttcgagttgg ctgccaatat
    31081 tggtactcca aaagacgttg aaggtgttaa caacaacggt gcagaagctg ttggacttta
    31141 ccgtacagag ttcttgtaca tggattctca agacttccca actgaagatg agcagtatga
    31201 agcatacaag gctgttcttg aaggaatgaa cggtaaacct gttgtcgttc gtacaatgga
    31261 tatcggtgga gataaggaac ttccttactt cgatatgcct cacgaaatga acccattcct
    31321 tggattccgt gctcttcgta tctctatctc tgagactgga gatgctatgt tccgcacaca
    31381 aatccgtgct cttcttcgtg cgtctgttca cggtcaattg cgtatcatgt tcccaatggt
    31441 tgcgctcttg aaagaattcc gtgcagcgaa agcagtcttt gatgaagaaa aagcaaacct
    31501 tcttgctgaa ggtgttgcag ttgcggataa catccaagtt ggtatcatga tcgagattcc
    31561 tgcagcggct atgcttgcag accaatttgc taaagaagtt gacttcttct caattggtac
    31621 aaacgacttg atccaatata caatggcagc agaccgtatg aacgaacaag tttcatacct
    31681 ttaccaacca tacaacccat caatcctacg cttgattaac aatgtgatca aagcagctca
    31741 cgctgaaggt aaatgggctg gtatgtgtgg tgagatggct ggtgaccaac aagctgttcc
    31801 acttcttgtc ggaatgggct tggatgagtt ctctatgtca gcaacatctg tacttcgtac
    31861 acgcagcttg atgaagaaac tcgacacagc taagatggaa gagtacgcaa accgtgccct
    31921 tacagaatgc tcaacaatgg aagaagttct tgaacttcaa aaagaatacg ttaattttga
    31981 ttaatcgaaa agtccctgca actcagttac agggactttt tttgatattt taaaaagaat
    32041 tttcaagaaa atctttctta taaaaagtcc aaccttgaaa aagtagtggt cagaacaaaa
    32101 aatacttaaa tggttcataa aattcttgac aagttggaga tttaggagta aactattaac
    32161 cagttaagta atagagagga gtttctgcaa tttagaaatg aattgcaact agaaatatca
    32221 aatagaaaga gagtttcgat gaaaattaat aagaaatacc ttgttggttc tgcggcagct
    32281 ttgattttaa gtgtttgttc ttacgagttg ggactgtatc aagctagaac ggttaaggaa
    32341 aataatcgtg tttcctatat agatggaaaa caagcgacgc aaaaaacgga gaatttgact
    32401 cctgatgagg ttagcaagcg tgaaggaatc aatgctgagc aaatcgtcat caagataaca
    32461 gaccaaggct atgtcacttc acatggcgac cactatcatt attacaatgg taaggttcct
    32521 tatgatgcga ttttcagtga agaattactc atgaaagatc caaactataa gctaaaagat
    32581 gaggatattg ttaatgaggt caagggtgga tatgttatca aggtagatgg aaaatactat
    32641 gtttacctta aggatgctgc ccacgcggat aacgtccgta caaaagagga aatcaatcga
    32701 caaaaacaag aacatagtca acatcgtgaa ggtgggactc caagaaacga tggtgctgtt
    32761 gccttggcac gttcgcaagg acgctatact acagatgatg gttatatctt taatgcttct
    32821 gatatcatag aggatactgg tgatgcttat atcgttcctc atggagatca ttaccattac
    32881 attcctaaga atgagttatc agctagcgag ttagctgctg cagaagcctt cctatctggt
    32941 cggggaaatc tgtcaaattc aagaacctat cgccgacaaa atagcgataa cacttcaaga
    33001 acaaactggc taccttctgt aagcaatcca ggaactacaa atactaacac aagcaacaac
    33061 agcaacacta acagtcaagc aagtcaaagt aatgacattg atagtctctt gaaacagctc
    33121 tacaaactgc ctttgagtca acgccatgta gaatctgatg gcctt
//
LOCUS       DAVKLX010000022        33133 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKLX010000022 DAVKLX010000000
VERSION     DAVKLX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33133
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.022"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            161..1672
                     /locus_tag="VW325_001076"
     CDS             161..1672
                     /locus_tag="VW325_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9734492.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            1694..3025
                     /locus_tag="VW325_001077"
     CDS             1694..3025
                     /locus_tag="VW325_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9734493.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(3430..3720)
                     /locus_tag="VW325_001078"
     CDS             complement(3430..3720)
                     /locus_tag="VW325_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734494.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(3708..3960)
                     /locus_tag="VW325_001079"
                     /pseudo
     CDS             complement(3708..3960)
                     /locus_tag="VW325_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            4164..4241
                     /locus_tag="VW325_001080"
                     /pseudo
     CDS             4164..4241
                     /locus_tag="VW325_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4359..5243)
                     /locus_tag="VW325_001081"
     CDS             complement(4359..5243)
                     /locus_tag="VW325_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9734495.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(5388..6539)
                     /locus_tag="VW325_001082"
     CDS             complement(5388..6539)
                     /locus_tag="VW325_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9734496.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMAINGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGYDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(6685..8451)
                     /locus_tag="VW325_001083"
     CDS             complement(6685..8451)
                     /locus_tag="VW325_001083"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9734497.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAILGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(8534..11650)
                     /locus_tag="VW325_001084"
     CDS             complement(8534..11650)
                     /locus_tag="VW325_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9734498.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            complement(11660..13954)
                     /locus_tag="VW325_001085"
     CDS             complement(11660..13954)
                     /locus_tag="VW325_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9734499.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(14013..14813)
                     /locus_tag="VW325_001086"
     CDS             complement(14013..14813)
                     /locus_tag="VW325_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9734500.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(14810..15583)
                     /locus_tag="VW325_001087"
     CDS             complement(14810..15583)
                     /locus_tag="VW325_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9734501.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(15608..16078)
                     /locus_tag="VW325_001088"
     CDS             complement(15608..16078)
                     /locus_tag="VW325_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9734502.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(16069..16500)
                     /locus_tag="VW325_001089"
     CDS             complement(16069..16500)
                     /locus_tag="VW325_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9734503.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            complement(16493..16933)
                     /locus_tag="VW325_001090"
     CDS             complement(16493..16933)
                     /locus_tag="VW325_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU9734504.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            complement(16945..17583)
                     /locus_tag="VW325_001091"
     CDS             complement(16945..17583)
                     /locus_tag="VW325_001091"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU9734505.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            complement(17698..19101)
                     /locus_tag="VW325_001092"
     CDS             complement(17698..19101)
                     /locus_tag="VW325_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9734506.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLVDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPLLTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            19277..20050
                     /locus_tag="VW325_001093"
     CDS             19277..20050
                     /locus_tag="VW325_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9734507.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(21220..22725)
                     /gene="adcA"
                     /locus_tag="VW325_001094"
     CDS             complement(21220..22725)
                     /gene="adcA"
                     /locus_tag="VW325_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9734508.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSVKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQETLFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(22735..23541)
                     /locus_tag="VW325_001095"
     CDS             complement(22735..23541)
                     /locus_tag="VW325_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9734509.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(23534..24238)
                     /locus_tag="VW325_001096"
     CDS             complement(23534..24238)
                     /locus_tag="VW325_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9734510.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            complement(24238..24678)
                     /gene="adcR"
                     /locus_tag="VW325_001097"
     CDS             complement(24238..24678)
                     /gene="adcR"
                     /locus_tag="VW325_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9734511.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(24833..26101)
                     /gene="dltD"
                     /locus_tag="VW325_001098"
     CDS             complement(24833..26101)
                     /gene="dltD"
                     /locus_tag="VW325_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9734512.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(26094..26333)
                     /gene="dltC"
                     /locus_tag="VW325_001099"
     CDS             complement(26094..26333)
                     /gene="dltC"
                     /locus_tag="VW325_001099"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9734513.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(26347..27591)
                     /gene="dltB"
                     /locus_tag="VW325_001100"
     CDS             complement(26347..27591)
                     /gene="dltB"
                     /locus_tag="VW325_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9734514.1"
                     /translation="MMEFFQQLPHLEPYGKPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQFLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(27588..29138)
                     /gene="dltA"
                     /locus_tag="VW325_001101"
     CDS             complement(27588..29138)
                     /gene="dltA"
                     /locus_tag="VW325_001101"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9734515.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVFTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEDLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(29159..29290)
                     /locus_tag="VW325_001102"
     CDS             complement(29159..29290)
                     /locus_tag="VW325_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9734516.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(29604..30782)
                     /locus_tag="VW325_001103"
     CDS             complement(29604..30782)
                     /locus_tag="VW325_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9734517.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            complement(31238..32515)
                     /gene="ltrA"
                     /locus_tag="VW325_001104"
     CDS             complement(31238..32515)
                     /gene="ltrA"
                     /locus_tag="VW325_001104"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEU9734518.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAASKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKFKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
BASE COUNT        10060 a         7043 c         5606 g        10424 t
ORIGIN      
        1 tagaaattat tcttgaaaac atgtacaata tttcaaaaga tattagtttt ttagacttta
       61 atatggtaaa acaaatataa atagaaagcg ttttcttaaa tgtttattta agaaagtagt
      121 tggtttttta cactttgtta gacatcagga ggaaaaacaa atgagtgaaa aagctaaaaa
      181 agggtttaag atgccttcat cttacaccgt attattgata atcattgcta ttatggcagt
      241 gctaacttgg tttatccctg cgggggcctt tatagaaggt atttacgagg ctcagcctca
      301 aaatccacaa gggatttggg atgtcctcat ggcaccgatt cgggctatgc taggtactca
      361 tccagaggaa ggttcgctca ttaaagaaac gagcgcagcg attgatgtag ccttcttcat
      421 ccttatggtt ggtggtttcc ttggcattgt caacaaaact ggtgctcttg acgtagggat
      481 tgcctctatc gtgaagaagt ataagggccg cgaaaaaatg ttaattttgg tactgatgcc
      541 tttgtttgcc ctcggtggta caacttatgg tatgggggaa gaaacaatgg ccttctatcc
      601 actccttgtg ccagttatga tggccgttgg ttttgatagc ctgactggtg ttgcaattat
      661 tttgctcggt tctcaaatcg gctgtttggc atctactctg aatccatttg cgacaggtat
      721 tgcttcagcg actgcgggag ttggtacagg ggacggtatc gtacttcgtc tgattttctg
      781 ggttaccttg actgctctta gtacttggtt tgtttaccgt tatgcggata agattcaaaa
      841 agatccgact aagtcactgg tttatagtac tcgcaaagaa gatttgaaac actttaacgt
      901 agaagaatct tcatctgtag aatctacact tagcagcaaa caaaaatcag ttctcttctt
      961 atttgtgttg acattcatct tgatggtatt gagcttcatt ccatggacag accttggcgt
     1021 taccattttt gatgacttta atgcttggtt gactggtctt ccagttattg gtaatattgt
     1081 cggttcatct acttctgcac taggtacttg gtacttccca gaaggcgcca tgctctttgc
     1141 ctttatgggt atcctgattg gtgttattta tggtcttaaa gaagataaga ttatctcttc
     1201 cttcatgaat ggtgctgctg acttgctcag tgttgccttg atcgtagcga ttgctcgtgg
     1261 tattcaagtt atcatgaacg acggtatgat taccgataca atcctcaact ggggtaaaga
     1321 aggcttgagc ggtctatctt cacaagtctt tatcgttgta acttatatct tctatctacc
     1381 tatgtcattc ttgatcccat cttcatctgg tcttgccagc gcaactatgg gtatcatggc
     1441 tccacttgga gaatttgtaa atgtccgtcc tagcttgatt atcactgctt accaatctgc
     1501 ttcaggtgtc ttgaacttga ttgcaccaac atctggtatt gtgatgggag ctcttgcact
     1561 tggacgtatc aacattggta cttggtggaa attcatgggc aaactcgtag tcgctattat
     1621 tgtagtgacc atcgcccttc ttctccttgg aaccttcctt ccattcctat aaaatagtga
     1681 gtgaggtgat tccatgaaaa tagatataac aaatcaagtt aaagatgaat ttcttatatc
     1741 attaaaaacc ttgatttcct atccttcagt actcaatgaa ggagaaaatg gaacaccttt
     1801 tggacaagca atccaagatg tcctagaaaa aactttagag atttgtcgag acataggttt
     1861 cactacctat cttgacccta aaggttatta cggatatgca gaaatcggtc agggagcaga
     1921 gcttctggcc attctctgtc atttggatgt tgttccatca ggtgatgaag cagattggca
     1981 gacaccgcca tttgaagcaa ctatcaaaga cggctgggta ttcggacgtg gtgtccaaga
     2041 tgataaaggc ccttcgctcg cagctctcta tgcagtaaaa agcttgctgg accaaggtat
     2101 tcagttcaaa aagcgcgtac gctttatctt tggtaccgat gaggaaaccc tctggcgctg
     2161 catggcacgc tacaatacca tcgaagaaca ggccagtatg ggctttgcac ctgactcatc
     2221 ttttcctctg acctatgctg aaaaagggct tctacaggtc aaacttcatg gcccgggatc
     2281 ggatcaacta gagcttgaag taggaggcgc ctttaacgtt gtaccagaca aggccaacta
     2341 ccaaggtctc ctctatgaac aggtttgtaa cggtctcaaa gaagctggtt atgattacca
     2401 aaccactgaa caaaccgtaa cggttctcgg agtgccaaag catgctaagg atgctagtca
     2461 aggtatcaat gctgtcatcc gactagctac cattcttgct cctctccaag aacaccctgc
     2521 tctcagtttt cttgcaacac aagcaggtca agacggcaca ggaagacaaa tctttggtga
     2581 tatagcagat gaaccttctg gtcacctatc ctttaatgtc gcaggtctca tgatcaatca
     2641 tgaacgttct gaaatccgta ttgacattcg gactcctgtc ttagctgaca aggaagaact
     2701 agtagagttg cttacaagat gtgcacaaaa ctaccaactc cgctacgaag agtttgacta
     2761 tctagcgcct ctatacgtcg caaaagacag taaactcgtt agcacactga tgcaaatcta
     2821 ccaagaaaag actggcgata acagtcctgc tatttcatcc ggtggtgcca cttttgctcg
     2881 caccatgcca aattgtgtag ccttcggcgc cttattccca ggagcgaagc agacagaaca
     2941 tcaggcaaat gaatgtgccg ttctagaaga tttgtaccgt gctatggata tttatgccga
     3001 agccgtctat cgacttgcaa cttaatcagg caactgtttc tacaaaaaaa atcgaccgat
     3061 taatgaactg caccccaaaa gttagacaga ataaatctaa cttttggggt gttttattat
     3121 gaaattgagt tatgaagata aagttcagat ctatgaacta agaaagcaag gacaaagctt
     3181 caaacagctt tcaaaaagat ttggtgtgga tgtttctggt ctaaagtcat ctgaatcttt
     3241 gagatgagct ttataaattg cttttttcag tttttgcact ggtatttcga taaactcaaa
     3301 ctttttagcc gttgtattgc ctgattttat agtatgttga aactagaata gtacacctct
     3361 acttctaaaa catttttaga aatcaatttg actgtcctga ttgatttgtc ctgttcttat
     3421 ttcattttac tatatttgag ccacttcgtc tttaacggct ttattcataa gctcttgtaa
     3481 tttttcttta ctatcaatta cttctgattt tccgttgtaa tttattgtaa taggttttaa
     3541 cttacctaat ttctcgacac gctcattaat ttgatctttt ttgaaggctg catatgtttt
     3601 tcctaagatt ttttcgaaaa tatatttatc agatagcggt ttgccttctt cttcagcttg
     3661 gtttttgtat tgatttgaaa cataaggaac aaatccttca tagtaaccta atgctcccat
     3721 aagttcaaaa gcttgttttc taattcaaac cattgcaact cagatttcag cttttcagat
     3781 aaatcctgct catccaaata atgacttgaa attagtgctg aactcgtttc tgtatcctgt
     3841 acaggctgag cacccatacc agcaaaaaaa taaactcgtt cctagcaaga ccgaacaagc
     3901 tcctattgca tatttcctca aagaaaaacg ctgctttctc tcaaattgaa attctttcat
     3961 cccatctccc atcattcatt attactgtat attttgtata tcagaaatag tttgtattca
     4021 caaatctttc tagttattcc cttatcattc ctaattaagg gagataacat acaataattt
     4081 ttagttaaat gtatatcgat gttttttgtt tttcttaata aacgcaatac aaaaagagcc
     4141 tgttgccaag ctctttatac tcaatgaaaa tcaaagagca aactaggaaa ctagctacag
     4201 gttgctcaaa acaccgtttt gaggtagtag ataagactga cgaagtcagc tcaaaacact
     4261 gttttgaggt tgcagataga actgacgaag tcagtaacat ctatacggca agacgacgct
     4321 gacgtagttt gaagagattt tcgaagagta ttagtctatt atttcttctc agcgcgaagg
     4381 gctgacaaga tttgtgtacg gatatcatcc acaccatttg gagtatttgg taaaaagatg
     4441 gtttgatttc ctttagaggc aaaggtattc aaggtatcca aatactggtt ggtcaagagg
     4501 atagacatga tttgttcttc tgtcatgcca acattggctt ccttgagttc ggtgatagac
     4561 tctgccaatc catccacaat cgccttacgt tgttgggcaa tccccacacc atgaaggcgg
     4621 tctttttctg cttcggcttc agctgcagtg acaattttaa tcttgtcagc ttccgccaat
     4681 tcttgtgctg cgacccgctt acgttgcgcc gcattgattt cattcatgga ttgcttaact
     4741 tctgcatctg gttcgacctt ggtaatcaag gttttcacga taatgtagcc gtaagtggtc
     4801 atttcttctg ctacttggtg ttgaacctca agggcaatct catctttttt ctcaaacaat
     4861 tcatccaagg ttaattttgg aacagaagag cgaagagcat cttcgatata agatttaatc
     4921 tgagattctg gacgtatgag tttatagtaa gcatctgtca cgctctgctc gttgacacgg
     4981 tactgagtcg ctacattcat cataacgaac acattgtcct tggtcttagt ctcaaccaca
     5041 atatcacttt gcaacaagcg caactgaatc cgtgctgcaa tcgagtcaat cccaaaaggc
     5101 aagcgaatat gaataccgct attagcaacc ttttggtatt tcccaaagcg ttcaataatc
     5161 gccaccgact gctgacgaac cacataaact gtactcagtg tgactatcac caataggagc
     5221 acacaaacaa tcagaaaaat catgaaaaat attgccataa tggaacctcc acaagtattt
     5281 ttctagtatt atagcacatt taaagaaggc tgtgccgttt ttactgcgat ttttcctgaa
     5341 atgtcaataa ttagaggtga attgtcctat tgtcgtctaa tctcttgcta aaataactct
     5401 ttataaaagg caatcgtttc ttctaaggtt ggcataaatg gatttcctgg tgcgcaggca
     5461 tcaatcaagg cattcttaga aaggtattca aagtcgaaat ctttttcttc aataccaagt
     5521 tcagtcaatt tcttaggaat acctactgtc tcagaaagct tctcaatctc agcaatcgca
     5581 taatcggcac actcttgatc tgatttacct tccacatgaa ggcccaaggc tttggcaaca
     5641 ttgcggaaag cttctggtac acgtttagca ttttcacgtt ctataactgg tagcaacatg
     5701 gcacagcaaa caccatgtgg caaattatat actgcaccga gttggtgagc cattgagtga
     5761 acataaccaa gaccagcatt attaaagctc ataccaccaa ggaagatggc attgaccata
     5821 ccttcacgcg cttcaatatc gtatccattt tctacagcac gaggaagata ttccttgata
     5881 agctcaatag caccaataga cagtttcttg gttacatcat aagcaccagg tgttaccaaa
     5941 gcttcaacag cgtgagtcag agcatccata ccagtagcag cagtcaggcc tttaggtttg
     6001 gaaagcatga gttccgggtc attaacagag ataagagcaa ggctattctt gtcaaccatt
     6061 accatcttaa ccttgcgttc ttcatcagta atcacatagt taatagtgat ttctgcagaa
     6121 gttcctgccg ttgtattgat agccacaact ggcaagcctt ttttagcaga cttatgaagc
     6181 ccttcataat cctgtggttt tccaccattt atagccatga tagaaataca actagctgca
     6241 tcctgtggag aacccccgcc aagactgatg ataaagtcac agccatgttc ttgcaaagca
     6301 gctaccccat ctgtgacatt tttacaagta gggtttggct ctacatcgct aaagatgaca
     6361 tattcgattc cttctgtatc cagtggtttt aaaaccttag gcaaaatatc actgccttcg
     6421 atgtacttat ctgtaaccaa aagtgccttt ttatagccaa gttccttgat ataaggaccc
     6481 acttcattta caacaccttt accaataagg ttgactgccg gaacataaaa tgtagccata
     6541 taattttcct cctgcgcctc cgcgctatct atttaacatc attatattac tttttgtatt
     6601 cgttttcaat tatttgtgaa ttgtttaaca aatcttttta aaaggatagg agggacgtaa
     6661 gttcacctcc tatataaaca agttttattt atgtagtggt cccaacaact gacatgcacg
     6721 atagtctgct gattctagat cttctgttcc aaataaggac caatttttag gtctaaagat
     6781 atcttcctca ggtacattat gcatattgac aggaattctc aacatagaag ccaaggtaat
     6841 caagtctgct ccaatgtgtc catatgttat ggctccgtga ttagctcccc aattattcat
     6901 gacgtcatag acagacttga aagcaccttt tcctgtcaaa cgtggagcaa accaagtagt
     6961 tggccatcct ggatctgtac gattatctaa agtatggtga acatcttcag gaagttcaag
     7021 tgtataacct tctgcaattt gtagcactgg accaacccct tttagaagat tgagacgtac
     7081 cattgttact ggcatatccc ccttcgtcaa gaaacgagtt gagaatcctc ctccacggaa
     7141 gtattcgcgg tttgctggtg ggaagtctgt attttcaagc atagcctgca cttcactttc
     7201 ttccaactcc cagaatggtt tcataacagg tttgccatct cgagtagctt gacctgtacc
     7261 atccaatgta caagaaccag agttgattag atgtaagaag ccagctgcag cacgaccctc
     7321 taaagtatgt cccgttacac gtttaacagc ctctgggctc caataagtac gcacatcagc
     7381 aaagatttgt ggagtatttg ttaatagata attaaagagc atagacacac catttagtga
     7441 atcattctct gtcgcaaata caaatggttt tcgaatacca ttccagtcaa actgagtatt
     7501 gaggaaagtt tccataaagt ccccatttgg gaaatgatct gtccactggc gttgaccttg
     7561 gaaaccagct actaaagcat ggtgaccaac tgcttcttcc tcaaaaccaa gctcagcaag
     7621 tcttgggtta ccaaccatta agtcacgtcc aatcatgaac atcttaataa caaattccca
     7681 ttgtctatct ttttcttcac ggcttaaaac aaggtcttca cggttatggt cgaatccttc
     7741 ttttacgttt tctttcaccc atttgagcgc acgttcgaac tcttcagggt cgtaaatact
     7801 acggtccata cggcgcgtga actccgtcat atctaccgat tcatttcgca ttcctaggta
     7861 ttcttggaag aaatccggat ttacaataga accaccaatc cccatcgaaa cactacccat
     7921 tgataggtaa gcagtgtctc tcatcaagcc agttgcaaga gctgcacgcg catagcgtaa
     7981 aagtttttct ttgacatctt ctggaatatc tgtgtcacta gcttcctgaa catctcttcc
     8041 ataaatccca aaggctggaa tccctttttg agcatgtgaa gctagtacag ctgcaagata
     8101 gacagctcct gggcgttctg tcccattaaa tcccaaaata gcatgaggaa tatctggaga
     8161 catatccata gtttcactac cataacacca gcatggtgta actgtaattg ttgcgcaaac
     8221 atttgatttt ttaaacaact catgggaagc tgcagcctct ggtacacggc caatagtaga
     8281 tggagaaatc acgcattcca caggttcccc atctggatat ttcaatgtgc ttgaaatcaa
     8341 atctgccaca cttttagcca tgttcattgt ttgcacttca agtgattcgc gtacaccttg
     8401 acgacgacca tcaatagtcg gacgaatccc aatacgtgga tgttgaatca tactattttc
     8461 ctcttatttt tctgtttctt tcatactacc atctccatta tatttacggt agcctgggtg
     8521 ataattttta aaattactta taagtataaa cctctacctc tgctaaactt aaaggaattc
     8581 ctgcgtttct caaagaaatt ctaacctttt ttcccttcag tccttttaag tctatggata
     8641 gattattgct ttctaaactg tcaatatgtt gtttaaaaac ttcatgatta tttgaatcat
     8701 atataataat atcaaaattt gataatctct gaatttcagc atctgttcta ttataaataa
     8761 ttaacttttc taattcttcg gtttgtgcta aatctatctc ccaccatgaa ggagaatctc
     8821 ctttggtatg agtcactgaa tgatgaccat aatctccgtt tttatttcca tcaactgcta
     8881 gacgagaaaa accattgtaa tccgttgaac tctgtcgagt ttctttggtt aatgcaatat
     8941 tagaaagttt cttcggagtt ctctttgaac catagacttc aacttccgct aaacttaacg
     9001 gaatagctgc tgattttaat tctattttga ctagcttagc ccctacagaa ggtactttta
     9061 aagataacaa accatcaact actttatcaa aatgtcttct aaaaacttct tctcctgatg
     9121 aagatagaaa aataacatca aaattagata aacgctgagg ttcggcatct gttctattat
     9181 aaatatcaac tttagaaacc gtaaactctt ctcccagatc gacctgccac caagcgttag
     9241 aatctgcctt agtatgagtc accgaatgat gtccgtaatc tccatcttta ttaccatcaa
     9301 ctgctaaagc agctacaccc tcataattgg ttgaactttg agtagctacc ttatttgtag
     9361 agactacttt atcttctgtt attttatcta tatcctcttc agattgctta ccatctgatt
     9421 ctctaaaaac ctctacttct gctaaactca aaggcactcc actcgttagt aatttaactt
     9481 taatatacct tgctcctttt tctttgaaat ctagactaac agattcaccc gacaaattat
     9541 taacatgttt cttagcaact tcgtttctat tattgtcata tagaatcaca tcaaaattag
     9601 atagacgttg agtctcagca tctgtgcgat tataaatttt aacaagccca actttatcca
     9661 tttttttcaa atcgacttcc caccaagagg gattatctgc cctagtgtgt gttaccgaac
     9721 cagagttaaa attaccattt ctgttaccat ccacagctct attagggtct ccattgtaat
     9781 ctacagaact ttgtgttgtt ggttttgcat atgctatatt caaattacca tcattaaatt
     9841 tatctggtgt tgggaaagag actggacctg tcccctcagt atttatagac atctctacca
     9901 ttccattatg attaaccgta atggtataaa catgggtatt ctcatcccaa ttttctgtat
     9961 aagtataatg atttttatcg ccacttatat ttatctgagg tttatgacca gtatgccctt
    10021 ttaatgtaag tgttgttgtc ctaaagtcat tatctacagg attgatggaa taatttttgc
    10081 ttatccagtt cgctaactct aattcttctt tcttccaact ttttgatgca tcaaaatttc
    10141 ctgataatgc ccacatagct gtcttatctg tcctgtaatt attcaataaa atatgtaaat
    10201 tatttggatt ttctttaaca acagcgtaag tatgtggcgt caaatctaac gataacgact
    10261 ttgtattatt agtatacata ggcaacatta cttgctgatt tttattgata ttagcattac
    10321 tattataaat ataccaggaa ttacctacac gctgagcata cccatctcct tcataaagtt
    10381 ttggataaag cgagtttaaa tagttgactt tactagacaa ttcctcacta tttttagtca
    10441 aaatttttgc attagggaat atagatgaaa tcttttcctt atcaattttc tcatgtatta
    10501 caggaagaat atgatatctc ccattattat ataaaggcat tgtttcatca ttcgaataaa
    10561 gtccttgata aaatccgttt aatgaactaa tcctaccttc tccattccaa aatacagctt
    10621 ttgttctatt tacaacttct tccttacttg gagctggatt ttgtatagca tgtctaaaga
    10681 aaggaataat acctttagta aatgctggag ttggtacatc atttgtcata aatgtatacg
    10741 cggcacattc gaaattataa actgtgcctc ccccagtata tacattcatc atttccatag
    10801 caatcattga ttctggttcc gatgcatagg atctcatatc cctagctctt ccagtttcaa
    10861 atgtgtttgt ataatgtttt tcccaccatt tccatgtatc tgttgatgag ccccagttat
    10921 cacataagcc actcaaccaa aatccactaa cgatagaatc tgtacccgca tcatctctta
    10981 ttggcgtatt tttagttgcc aacaccaaat ttttatgata tttttgactc gcttcaaaga
    11041 atgtcggatc attcataata gtttcccaga accatttttc atgatcatgc cagataaaat
    11101 gcgctccata tttggcacaa acttccaaat atttagcact atgcggagct aactggttat
    11161 tgtaaatcca ataattctca atatttaaaa caccttttaa cacactatac ttttggaatt
    11221 gttcatctaa ccactctgga ggaactgtat tacgctctcc agccgacata ataaccaaga
    11281 aaactggaat gtttagactt tgagcttcct ccaacatctt gacataccat tctctcaatt
    11341 ctttcgtatc tcgtggaata caacttgttg gtttacagac ttttgcagga tgaagttcaa
    11401 tcgctgcata tggctttaca tcttcaggaa tagcttccca tgctcccttc aatgtgttcc
    11461 cccaccataa tccgttacca ttatcattgc catacaacgg agaaagcaac aatggcgatt
    11521 cattattaat cgtcgttctc atttgaacac gattatcagc cttaactcct gctaacaatc
    11581 caaaacataa catcccaata catacaaaca atattgtgat taattttctt tttatttttt
    11641 ctttattcat gacttatacc taaaaattta actcaataat tttttctgaa ttaaatacca
    11701 attcaatatt ttgtacatca gtatttttgc catatattct tactcttact ttttgatctt
    11761 tgtttcctcc ttccaatttt aggaatgtta tatccccatt tttccaagca aacgatacct
    11821 tatatcctcc tcttactctg aaacctttca cttctccttc tgaccaagca gaaggtaaag
    11881 ctggaattag tgataaccaa ttatgatggc tctgtactaa taattcacaa attccactca
    11941 ccaaacctaa attaccatca atttgaaatg gtggatggtc aagaaataaa ttgccaagag
    12001 tcgcattatt taacaaacca ttaatctggt tataagcagg ttcaccttga tatagtctcg
    12061 caaaaaaatg aatcagccat gcagcactcc aacctgtttg tgtactagca tgcaaaccac
    12121 ttactaacca attattaatc gcttgctccc tctcctgtga agataaaaaa ttagcgtttg
    12181 ataatctcct attgatagtg attttagctg cttctgctaa ttccggagtt ttatgaatat
    12241 caatctcatt ataaggataa agcccaaata gaggtgaaat gtgtctatgc ccaggctcta
    12301 cttcttcata atcttctaac cattcttgga tttgcccatt actacctatt tttgttttag
    12361 gtagtttctt ttttaactcc ttcacacgac taataaaatc cgaattgtct cctaattgtt
    12421 ttgcaatgcc aatgcatgaa tcacaaaaat atcttagaat ttgattatca attgtagatg
    12481 atagacaagc atttccttca ataccatttt ttaagcgata tttattttcc ggtgagacac
    12541 ttggacctgt catcaagtag ccatccacct caaataaata atcttcaaag aaaagaaatg
    12601 cttcttttat catttcaaaa tgttccgtaa gaatacgctc atcttggaaa tataaatagt
    12661 gttcccaaat atgagtacat aaccatggaa tagttaatac ccaaattgca gcccccatgg
    12721 catgagattg gggagccgta tcgccaaaac catccgtatt atgatgtgct gtaaaacctc
    12781 tagctccata cattttctta gcggttagtc ttcccggttc tctcattctt tcgagcatat
    12841 caaataatgg atattctact tctggtaaat cacatggacc taccatccaa taattcattt
    12901 gagtattaat attaatcgta tatttagaac cccaaattgg atttaattca tcacaccata
    12961 ttccttgaag attggcaggt aaaccattcg gttgactaga cgatattaac agatatcttc
    13021 cataatgaaa taacaagtta gtcaagtagt tactatactt tttagtgttt tcaagaagta
    13081 gattcgttgg aatgctaaga caatctttac tatagtctag tttaaaatca actctattaa
    13141 attgctcctg atattttttt acatgttcat ctttttctgt aaagtaatca atactactaa
    13201 attctccctg aagagaagaa atatctatat ttccccaata atccgtcatt gatttgagat
    13261 aaagaaatac ctctgtagca ttccgaataa ctattgtctc tcccaataca cttacttcac
    13321 catccgtaac cttagaatga catactactt taaactgaac accttttcta cctccagcag
    13381 aggccgacat taaaattgta cttgaatcca gtttagatac ttcatcatta aaccgtttat
    13441 ttctacccaa attaatgttt aaatttaatg tgttttgaac tgatgacact atacggcaac
    13501 ataaaatatt cttattaaaa ctcgtaaaat attctctttt tatttgtaaa ttacaactat
    13561 taggctcaaa cacaacatta gaaatagctg tatctaaatc tagctctctt tcatacaatg
    13621 aaagagcaca agactgaata tctatatgct caatgtaaag ttccccaagt aattcatagt
    13681 ggctttgatc tcttggggta gcaaacattg ttaactttat caattcttcg gctttctgaa
    13741 tttctccatc taaaagatat tcccgaattt ttttaagatg caatagtgag tctggattat
    13801 ttctatctga ctttcctcta taccaaatag tctcatcgtt tagttgaata cattcttttg
    13861 tagctgaacc ataaatcata ccacctaaat gaccgttccc aatcggcaag gcttcattcc
    13921 aatttgaggc agctttctta taccataatt tcactcttta cctctcttcc aaaatggagg
    13981 tgatatccca aataccacct ccacactttc tactacttca aaattcctaa aacaactagg
    14041 ataactccaa tagccataac actaaagatt agtacaaccg gattctttcc ttttctaatt
    14101 aatttacaca ttaataaagt caaagccata gggataaatc ctggaattaa ttttgtaatc
    14161 atttcttgaa cagaaataac aagttccccc tgcttaaatt ctaatccaaa attaatacca
    14221 accattgatg gaatcaaacc acccactagt actagcccta atgctgtcgc catactcgta
    14281 acgcgattca atgttccttt tgtattattt tcttggataa gactagaacc cttagtatac
    14341 ccatatttca aaccgaaata tttaacagga atattaataa tattaaacaa tattaaggcg
    14401 ataaagatac ctaaagcacc gccgtcttta gaataagacg caccaatact aaaacagata
    14461 ggaactagtg tcagccagaa caaactatct cctagaccag ccagtggccc catcaagcca
    14521 actttaatac cagtaattga agctgcacct tcatttcctt cttgttcttc catagcggaa
    14581 gtaactccaa gaataaatgg tgccgctgtt tgatgagtat tgaaaaactc aaggtgacgt
    14641 ttcattgcag gagaagctga atctttgtct gggtatagtt ttttcaatac tggaagaatt
    14701 gtatatagaa aacctaggtt ctgcattctc tcatagttcc atgaagaacc atacataaaa
    14761 cttcgtttga aaactttttt aagttcaact ttagttactt tattattcat catagatcaa
    14821 gatcctcctc aacatcacta cttgaagttg tttcttgtgg tttacttcca atcatatcgt
    14881 agaagtatgc taccgcaata ccaactagtg agatcccaat ggttgacatt cctccataca
    14941 cagaacaaat gaatccaatc aacaagaata cccacatatt tttcttgagc atcatattta
    15001 aaagcatacc aaaaccaatt gaaggtagta aagcacctgc agagttaagt ccctgcataa
    15061 cgattggtgg aaccttagca aacaagtctg ccacatattc ccctccaagc ataatagcta
    15121 ggaaaattgg tacaatttta aataacgtga ttaaaaccaa acttgaataa tgataaaaac
    15181 cgatctttga ataatctccg tttttagcat cattatcagc ttttttcaca aaataaacat
    15241 ctaaagtttt tgcaagaaca tccaactgtt gggtagcaac tgcaattgga actgctatag
    15301 cgataccagc agtttctcct tgaccagata aaataccaaa tgcagtacca ataatcgcac
    15361 ctgaaatagt atctggtgga gtgtaacctc caatacctgt tacaccgagc caagttaatt
    15421 caagagctga accaataaga acaccttggg tgaaatctcc taagactaag ccaaccattg
    15481 cacttgtaac taacggacgg tgaatcataa ataacggtcc attatagtca atagtggcaa
    15541 taatcccaac taatgtcacc agtaatgctt gtgtgaatag catatactgt acctccttaa
    15601 attattttta taaaaatttt tccaacattg taactttatc attaggaacc atttgaattt
    15661 caactctcgt tcccttatca actattttct taaaataata tttgtcttct tcacctagag
    15721 ctacacctcc cgttaccttt tcattttcct ccgttttact catctgtcct acattgaggt
    15781 actccaattt taaatttcct tcaatagaat catacacatc ttttggattt gtatatatca
    15841 gcattgttct cttttttatt ggcacagagt ttaaaacttc cacaaaccgt tttacactaa
    15901 agaaaacaat ttttaaacct accggtgcag aaatctttaa aatagattgt cgtgttttat
    15961 cttctgagat gcgatcatta acaatgataa cttgctcaat atcatacttt tttagccacg
    16021 tagtgacaac ttgaccatgt accagacggt catcaatacg aacaaactct atagacataa
    16081 atcctcttcc tcctctacgt taagttgttg tttaacatta aacaaactat tttgagcatt
    16141 ttgaacaatt tcttctaaat tgatttttga atcataactt gatattaatt ccactaggag
    16201 agggatatta aaccctgtta caatatcaac tgaatccaaa tttaaaaacc gtgacaaagc
    16261 cacattatta ggacttcctc caatcaagtc agtcaaaacg ataacctctt gatttgagtc
    16321 taacagttca tttacttgat ttttaaaata atgttcaaac tctacaatat tctctccagg
    16381 catcaaacct aatgtcctaa ctctatcatt tgttgtctca ccaacaaaca tttctgcggt
    16441 catgagagct ccgctagcaa aattaccatg actggcaact aaaattcctc tattaaacat
    16501 tttctctttc aacaactact cctttcttaa ggataatatt agcataaagt gaagtttctc
    16561 ctgtagcaac aatagcataa gctttcttac tacgttcata aaagtcttct cttctaagat
    16621 aagtaatcgt tttaagatct gtaccatgac cttcaatcat ctgtctatag gtaccccata
    16681 tcttaggaat atcatcaccc gaaacaacgt tcataaactg aattgaacta tcgacgtaac
    16741 tatctaatgg cattaaatac agaatggaat ctaataattc tggaatattt acgccatcac
    16801 aacgaattag cttatttgca catgaggcag aaggataatt cgcgtcagct aatactattt
    16861 catctccatg tcccatttcc attaaagtct tcaataaatc tggagaaata tttttcggta
    16921 tatgttttaa cattaatatc ctccctattt tctctgaccg tatgttttaa atttttctgc
    16981 cattaaatcc atctcctcat caggaagaac tactggctct ccaaaattct tagctaaaca
    17041 ataaattttt gcacaatatt caacttcttc aacaatatta aatgcattca ataaattttg
    17101 tgcacctgct aaaattccat gattcgctag taaaactgct ctacgacctt ccattgcttt
    17161 agctgcattc acagccaatt ctttcgtacc atatgttgca tactcagcta cccgaacatc
    17221 tttccctgcc actgcaatca tataatgact agccggaagt ggttctctga gacaagctaa
    17281 tactgttgca taagttgtat gagcatggat aattgcatcg atatcatcac gagtttgata
    17341 ttgaatcaaa tgcatatacc attcgctaga tggcaagcgt tctccctcta caacatttcc
    17401 attaatatcc atcactacaa tatcggattc tttgatttca aagaaatcaa tacccgacgg
    17461 ggtaattgcc atcaattgtt tttcacgatc gaaaacactg agattcccac ctgttccttt
    17521 cgtcaaatct gtttctacta gcttcttgcc atacttaatt aattcttgtt ttacatctga
    17581 cattcttgtc ctcctcatat caaaacacta aatatttttc aattcaccaa ttatcccccc
    17641 ttcttgtaca aaacttaaga agggggaggg aggcaaattt acaaactctc aatcttttta
    17701 attcttttgg gagtaataac tctctaattg tagaaactcc tcaataacat ggtgagcttc
    17761 ttccatccct tttaattgtc ccatagctat gagctgcaca acaatattcc ccacagctgt
    17821 agcctcagtc aaacctgtaa gaacctcttt accagttcta tcagcaatca tttggttaaa
    17881 gtaactggct ctagcacctc ctccaatcac atatatcctc ttataaacct tatgagttag
    17941 ttcttctagt aactctatcg ctttcctata cgtttcagct aggctttcat aaacaatctt
    18001 aaatagttgt ccatctgtcc actctctagt ttcatgatga taagctagat attctgtcaa
    18061 agtcttgtgc atatcagatt ctgttgcaaa ttcagttgat tcagtatcaa tcagaggaag
    18121 attttctttt tctttctcca ccattgtcct aatatcatca aaagaatagg ctttccctct
    18181 tcgttcaaat gaacgtctta gttcctctat gatccacaac cctgtacaat tcttcagaaa
    18241 tgtaatcact ccatctttac cgacttcatt tgtaaatcca taactgaagg attcggtagt
    18301 aagaagcggt gaagtaagtt ccactccaac caaagaccaa gtacctgatg aaataaataa
    18361 actaccttct gtcttaggta ctgagacaat cgcgcttgct gtatcatgac tacaaacatt
    18421 cacaacagga atatcgccta aaccatactc cttttttatc cttccaagaa catttccctc
    18481 tgaaacaatc tcaggaagta aagatgaatc caattcaaat agttttaaga tattctgatt
    18541 ccagttttga ctcctaggat caaataattg agttgttgaa gcaatgcttt tttctgtagc
    18601 aaacttacct gtcaagagat aattaaacaa atctggcatt aaaagaatct tattggtctt
    18661 atagaaagag tcaggagatt cttgacgtgc cttaaagagt tgaaacaagg tatttatctc
    18721 cataatctga tttcctgtct ctgaatacag tttttctaat tcagtcattt cagatatttc
    18781 ctttaacact ccctttgttc tttcatcacg ataatgaaca ggttgtaata acagcttacc
    18841 ttcattatca accagtccaa aatcaactcc ccatgtgtca ataccgatag ataaaatctt
    18901 gtaactagta ttagccaagc ggatactttc aagaatttta gctagtagaa agtcaatatc
    18961 ccaagataaa tgccctttta ctctaatagg tagattagaa aagcgattta tttcttccat
    19021 tactagttta ttttcagaaa ggtaaccaac gattgctctt ccagaagtcg cacctaaatc
    19081 tattgctaaa accgcttcca tttttcttct ctcttcgtcc ttgattaact ttattataat
    19141 ccccacgttt tattttttca atatgtgaac gcttacttga ataatgttca aatgttaacc
    19201 caatattcat aaaaataatt caaattcctc caatttgtgc tataatatag ttgaaatgat
    19261 aaattaggag gagcttatga taaaagatga acgtgtactt gaattgattg aaattattaa
    19321 aaagaaaaaa agaattgccg taaaagagct ggcagaaatc actttctcca gcacaagtac
    19381 cttacgtcgt gatttaattt tcttagaaaa tcaaggtctt atcaaaagaa agcacggata
    19441 cgtgaccctg tcctctatga acacaattga actttctcat caaatacggg aaggagaaag
    19501 tactaggcaa aaaagactaa tcgctagtct cgctaaagac tttattcggt caggtatgtg
    19561 tgtttatctg gattccagta cgactgtcta cgaactttgt ccctatcttt ctgaacttga
    19621 taatttgatt atttttacaa atggtttaca tactgcacaa accctatctg aaactgttaa
    19681 agatagctcc aaaatcttta tcacatctgg cgaggtcaaa catcaatcct gttccgtggt
    19741 caattacgat aaggaaaatt ctttattaga tcattttaat atcgatttag cattttgttc
    19801 agcaagaggt attgatgacc aatatgttta tgaagcttct ctcagccaag ctatttcaaa
    19861 aaagaatatt attgacaaag cccatgaaac catcttactg attgatagtt ctaaatttta
    19921 caagactgga ttttttaaaa ttaatcccct ctccaaatac acaaccttta tttcagacac
    19981 cgtgccagat caaaaattat tagacgcagt cgaattattt gatggagaat gggtttctga
    20041 tattcaatga aaatcaaata ggaagctagt ctcagcttgc tcacagtaca gatttgaaaa
    20101 ttgatcaagt cagctcgaaa cactgttttg aggttgtaga tagaactgat gaagtcagtc
    20161 acatatatac ggaaagacga agctgacatg atgtgaagag attttcgaag agtatcgttc
    20221 tgtctgctga ctgatttaca aatattcacc acatgacatt attttcttag caaccccaaa
    20281 aacagaggct ggacaaaaac tcaatttcag gagaaaatgg agtaaacctt cccacaataa
    20341 aacgcatagt atcaagtttt tgcacacctg atattatgcg tttttctgat tttaaagact
    20401 ttgtcgccta gaccctatat ttcaagaatt aagtgacctt gatgatctaa atgtattgtg
    20461 gtatactatt tttgtcatcg gttaccgatt acgttcctat gggttcgtga gaggaggtga
    20521 aactaatgag ccttatcata gagctcgctc ttactattat agcggatgtt atagctggaa
    20581 ttatcttgta tttcgtctgc agatggctag atagtaagga gtagaccgac tgactagcct
    20641 ataaacaccc gttaaatctc taagatacgt caaaaaagcc cttaactacg gccatagtta
    20701 ggggctttgg tgttctaatg aaccttatca attaactaca ttttagcata taatctacaa
    20761 tagttcaagc attaagtgag gttgatgatc taaatttagt gtggtatact atttttgtca
    20821 tcggttaccg attacgttcc tatgggttcg tgagaggagg tgaaactaat gagccttata
    20881 ccagagcttg ctcttactat tatagcagat gttatagctg gaattatctt gtatttcgtc
    20941 tgcaaatggc tagatggtaa gaagtagacc gaatgactag cctataaaca cccgttaaat
    21001 cgctaagata cgtcaaaaaa gcccttaact atggcactag ttaggggctt tggtgttcta
    21061 atgaacctta tacactaact acattctagc atataagccc agatatttca agagttttat
    21121 ttattttttc aggttccctt agttctgaaa ggtctataat gaagttagcc atctagtatc
    21181 aaaaaaccga ctagctctta tgaactagtc gatttctcat caatgcgcca acatttcttg
    21241 ggcgatttct tggccagata ggttatctgg atagtaggtt ggccaattat ccatttcttc
    21301 aaatagagtt tcttggcttg tacctccaaa aaagatatgg aaatgttctg ccttaactgg
    21361 ggcgatattg tggtcactaa actgaacata cttgaattgt ccagcgtcag catctgtggc
    21421 ttcaaagagg aaacgcacgc cacgattgcc tttcttgtaa gtcaaaattt tcttaccgac
    21481 atacttgtaa gtgtatttct tgctttgtcc accttgaaca aattccatag tattatcagt
    21541 aatgttaatc ttagtcacat ctgtctgata gccttttgta tagtaagcct tgtactcagc
    21601 ctgggtcatc ttaccagtca acttagcctt gtagtcaaag acttggtcaa acgtgccgtc
    21661 ttcaaggaaa ggataaactg attgccagtt acctgcatag tcactcaagg tgcggtcctt
    21721 gacagctgca tcctcgaagt aaccattttg gactgtcttg gtatcctctg ccttttcagg
    21781 ttcgattgct gggccttctt ggtctgttgt ttgtttcaaa gccttgaggt ttttctccat
    21841 cacggaaatg tagttttctc cagccttggt gtcctcttct gtcagacttt ctaaaggatt
    21901 gaggacatca gttttgacac ctgcttcttt tgaaagtgtg ttagcaaggg cttgtgaggc
    21961 attttcttca aaatagatat aggcgatttt atttttcttg acatactctg tcaattctgc
    22021 caagcgagca gctgatggct ctgcatctgg agaaaggcct gagattgcga cttgtttgag
    22081 tccatagtcc aaggcaagat agttaaaggc tgcgtgttga gtcacaaagc tcttttgttt
    22141 tgcttgagac aagccttctg cgtaagcctt atccaaggct tgcaattttt cgatataggc
    22201 agctgcattc ttctcaaagg tctctttttt atcaggataa tctgctgaca agctgtcacg
    22261 aatgtgctct actagtttaa tggcacgaac tggtgataac caaacatggg ggtcaaactc
    22321 atggtgatga ccttcttctc catggtcatg gtctccctct tcttcctcgc cacctggcaa
    22381 gagcaacata tcgcctgtcg ccttgatggt tttcactttt ttcttatcca aggtatctag
    22441 caatttaggt acccatgttt ccatgttttc attttcataa acgaaggtat ctgcatcttg
    22501 gattttggca actgccttga cagatggttc gtattcatga ggttctgtcc cagcaccgat
    22561 taggagttct acattagccg tatctcctgc aacttgcttg gtaaattcat agacagggta
    22621 aaaggttgtc acgatattga gtttaccatc tgcctgtttt tgattggaac aagccactaa
    22681 aaacaaggca catagactgg ctagtaataa gctaattttt ttcacgttcg tctcctattt
    22741 gataaaacgt cttactaaac tgattagtat aaagacagtt acaaaaataa tggtaatact
    22801 tgcacttgca ggtgtttctg catagtagga aatgtaaagt cctgctacca ttcccaaaaa
    22861 gccaatcgca ctggcaagca gcataaccga tttaaagttt ttccccagac gcagggcaat
    22921 actagctggc aagaccataa tggtcgatac cagaagagct cctgctgcag gaatcataag
    22981 ggcaatagcc acccctgtca ccatgttaaa aagaatggac atggtacgaa ctggcaagcc
    23041 atccacaaag gccgtatctt cgtcaaaagt taagatatac ataggacgaa gaaagagaaa
    23101 ggtcaaaatc aaaacaaccg ccgcaatgac aaagagggaa atgacctgtt cttcactgat
    23161 agtcacgatc gaaccaaaga gatattggtc caaactcatt gaactcgagc ttttaccctt
    23221 gctcatgaca atcagagaaa cagccagacc tgttgacatg aggatagctg tcccgatttc
    23281 cataaagctc ttgtaaaccg tacggagata ctccagaaag accgccgcaa tcaagacaat
    23341 ggcaatagta gaaacagttg gagaaatccc caaaaccaga ccaaaggcta cacctgaaag
    23401 tgagacgtgg ctaagggtat cactcatcaa actctgacga cgcaagatga ggaaggttcc
    23461 caataccggt gagaaaagac tcatagcaat aaccgccaaa aaggcgcgtt gtataaagtc
    23521 gtaagataat aaactaagca tggcccacct cctggccatt ctcatgaaca ttgaaacaac
    23581 gccatggcga gtcttggtta cggactagat gaatattgcg atccgcataa tccttaactt
    23641 cttcagggtc atgggtaatc atcaaaacag ccttgccatg atgatgggcg ctgtggtgca
    23701 tgagttcgta aaattcattt ttacttcctg catccatccc cgttgtcggc tcgtctagga
    23761 taaacacatc agggtcagaa gcaaacatac gcgcaatcac cgctcgctgc ttttgtcccc
    23821 cagatagaga ccccaagcgt ttgtctcgat gttcccacat gccaactgag tccagactag
    23881 ccttgatatg ctcctcatca tgagcattca aacgacggaa ccagcctttt ctcggatagc
    23941 gacccgactt gacaaattca tagaccgtac ttggaaaacc agcattaaaa ctggcaattt
    24001 gttgaggaag ataggctatt ctcaatttct taccttgcgt atttgtcttt gaaatagcca
    24061 cctttccaat gcgtggttgc agaattccaa gactggcctt gatgagcgtc gtcttagccg
    24121 ctccattttc cccagtcaag gtaacaaatt ccccactatc aacactataa ttgatatgtt
    24181 caagaacagg ctccttatca taatagaagg acaaatcctc taccgtaata tatctcatta
    24241 tttgatttct cctactaaag cagtcaaaaa ccgctgaatc actttttgtt catttggagt
    24301 aaactgagtc gccacttgtt cataggttaa aagtgtatgc tcatggtgat ggtggtgctc
    24361 ctcagcgatt ggacgagcca agtcagtcaa ctgataaaaa atcacacgcg catctttaga
    24421 atctttagat gtttccaaca tcccttcctt gaccaaagac ttaatggcct tggtaactgc
    24481 cgcctgactg acattgagac gacgggccaa ttctgaattt gttaaagatt cctctgacaa
    24541 gagcataaga atatgctcct gagtattggt cagggccacc tcgctagtgc aatgacctat
    24601 taggatttca tgctgatttt ccgcctgcaa aatcacctca ttcaaaaaag cattgatatc
    24661 ctttgctagc tgtctcatat ctgactcctt tccttttaga cttctctttt ttaagagaaa
    24721 aatactattc tttgacattt tgtttaccag ttaattatat cacaagcaaa aaaagagtca
    24781 agaaaaaacg tgaaaactag tttcattctt gaactcttct atattatatt atctattgaa
    24841 attctttgac atctccatca taagtcgccc aatctttgct gaaaaagcgc tcattcagat
    24901 ggtaagtcgg agctggtgtg ggattggata ggaaaggatc aactgccttg tcaaaagcca
    24961 accaacccaa ccaaccaagg tgaatggtgt ccttcataaa gaaaggctcc ccgccgtcct
    25021 tagaaaaatc tgctatattg gtaaaacctt gactttctaa ctggtagcga atcttctgca
    25081 ccgtttgttg gtacatatcc tctcgtagac cagcatagtc catccatttt ttattaacag
    25141 gtggaatgat aaaaatcggg tttaccttag atttagaaaa ctgtgttaaa accaactgca
    25201 agtcattata ctctggcgac ttgagatagg taaagctttt ctgagaatcc tttaatttct
    25261 tcaaatcctt cttgatctgc tcattataga aataattttc cattcccatc tcattattgg
    25321 aagtattttt ttcagcatct gctttgacaa catcttctat tgcttgataa gaaaactggt
    25381 ctggcaagat ttttaaatac ttagctacat gcttatcgta gttaacatag cctctaaccg
    25441 aaaactgacc aaaaaaggag gcttggcgtt cattaaaacg agccaataat tcaatcattt
    25501 cattgtctgc tgtcgacaat tctcctttac ttgccaactt ctgaaccagg tccttcatag
    25561 ctacgtttgg gaactgttgc agtaagcgag tcgctgcata ttgactagcc tgatccccag
    25621 attgatgttt cagaaaacta gtcaactggt ctccattaaa atactgctgg aaggctgctg
    25681 gatcatagcc atttttactg aaccactgag gtgagataac atacacaact tgtttattct
    25741 ccagctgtgg taacatctgt tgcattccaa aatattggtt aagcgatgca gctcccccct
    25801 gtcctaaaag ataaggacgg taggaacgat tgtatttctc agctaatacc gcaggatgag
    25861 caccgtcaaa acgaagccat tcactagagc caaagaaggg aacaaaacgc acatttggat
    25921 cagatagtgc tctgactttt tgacttcgct ccttaaaact atcgatagta gtagccactg
    25981 ctgaacgctt ttcagctcct agattatgat gcatctcagt aggataaaag aaaatgagca
    26041 gaaaaaccaa caaaccagcg atcaagaccg gtccgaagat catccataag cgtttaagca
    26101 ttttgtagct ccacaatacc agctatgatt ttattagctg tattccagtc gtcacgacca
    26161 aactctgtta cagggacacg aatgtcaaaa cggttctcaa tctccacaat caactcaacc
    26221 gttcccatac tatccaagac acctgcatca aaaagatctt catccatcat gtcagaaaca
    26281 tcttccataa acaactcatc aataatttca ataacttctg atttgatatc catattttat
    26341 ttccttttat tttttaaacc atagattatt caagaatcca gaaaagatta agaatgacaa
    26401 catgacaaca tggaaagtga caaccatgcc aagaaactga atccagcgat tctcaggtag
    26461 ggcagccttc cctgcttttt tccgttcctt attgagcgtt tttttcttgc gaacccaggc
    26521 atcattgatg accaagccta gtccatgaaa gagtccatag gcgatatagt accaggtcac
    26581 gccatgccaa aatcccataa tcagcatatt tacaatgtag gccatgcttg aggttacatt
    26641 acgattttta aagactttct ttctggttaa caccatcacc attcgcataa agacaaagtc
    26701 acggaaccag aaggacagac tcatatgcca gcgattccaa aactccttta aatcccttga
    26761 taaaaagggc ttgttaaagt tgatagggct acggattccc atcaagtttg agatggccaa
    26821 agcaaacata gaataacctg caaagtcaaa gaagagctcc agaccaaaag tatacataac
    26881 tgccaaggca tagagattaa agaagccacc tgactgcaag gctaaattct tcagaggagg
    26941 tagtaaggtc tctcctaaaa catgagctag gataaactta tacaaaaagc cccacatgat
    27001 atagcggaca gattcatcca gcatatccat caactcatct cgctcaggaa tagcctgata
    27061 attttcatta aatcgcttaa agcgatcgat tggaccactc gagaaagttg gcatgaagag
    27121 aaggaaacgg aggaattccc agagggtaaa atccttaatc actccatctc tcagctcgat
    27181 gacaattcca accgaacgaa aggtcaggta agaaattccc aagaacccaa gcaaagactg
    27241 cgttccattg atagctggtt gcaccttgac aaagataatc ggaagtaggg acagaaaact
    27301 aactaagtag aagacccact tgccatcctt gctttttcga taatgcttgt agaaaagcag
    27361 gagcaatatt tcccagcaaa ggtaaatacc caaggcagct agttgattgg tctttccacc
    27421 caccaacatg gtgacaataa agaagagact taccaacact tcataccagg caaagcgttt
    27481 cttgaaaaag agacctataa agatgggcaa ggttgcagca atcacataaa caaaatactg
    27541 aggtttgcca tatggctcta aatgaggaag ctgttgaaaa aactccatca tctcttattc
    27601 acctcgttaa tcaatccttt gatgtcaatc tttccatttg gagttagtgg caaactgtct
    27661 cggtaaagga atttagatgg catcatatag gacatcatga tgtctgtcag gtcttccttg
    27721 atggccttgg taatatcgat atctcgctca aactgctcac gaacaccgtc ttttaagatg
    27781 acataagcca atagattttg taccttgtgg tccttgttat agcgcggtac tgcgacagca
    27841 gattcgataa agcgagactt gttgaggttt tgagagacat cttctaactc aatgcggtaa
    27901 ccgttaaact taatctggaa gtccatgcgt ccgccgtaga gaagcaagcc ctcatctgtc
    27961 atggttccca catcgcctgt gtgataggct ggcagatctt caaactcaaa gaaggcttct
    28021 gctgtttttt caggattgtt catataacct tttgaaacag ctggcccaga aacaatgatt
    28081 tctccctgct caccatttgg cagtttattt ccttcctcgt caatgataaa ggttggagaa
    28141 tcagccttgg tatagccgat tggtaggcgt ttgagagtcg ctaacatctc gtctgtcacg
    28201 gcaactgctg acagagctac tgtcgcttct gttgggccgt aagcattgat gatacgggca
    28261 tttgggaaac gctcgcgcag tttttgagct gttttgaccg tcaattcttc accatcaaag
    28321 tagaaatgcg tgattccagg cattttctca ctgttgaagt attcagacaa catggccata
    28381 tctgcaaagg atggtgttga tgtccagata gcgattggca atgaaaagat agccgcaaag
    28441 agttgcttaa agtcctgagt gatgactgaa ggaagagtga aaagcgtacc accaagtgcc
    28501 aaggtcggtg cccaatacat gacagacaag tcaaaagaat aaggtggctg tgccagcatt
    28561 tgcggacgac tcggtgtcgc aaattcctta tccgtaatca tccagtttgt aaagctgagg
    28621 agattatcat gtgaaatctg cactccctta ggcttaccag tcgtaccaga agtaaagata
    28681 atgtagtaat tatcatctcc cttgactgga tgcgtgattt catagttatt cccttgggca
    28741 aaggcttctt gaacctgagc tagatttatc attggtgtaa aaacctgctc caagggaaag
    28801 gctgaaatgg caataatcaa gcttggctct gctacttcta aaatagctga aactcgctcc
    28861 aaggccgaat ggctatcaat tggaatgtag gcatgacctg acttagtcag cgctacaaag
    28921 gttgccaaca tttcatattc ttggccacca aaaacaacca caggagactt ctcaggcaag
    28981 cctagttggt caatgactgc agccaaacta tccgaatcag cctttaaatc gccataagtg
    29041 tgttcctgcc ccaaaacatt atagacagga tagctaggct gtgtctgagc aaaatgctca
    29101 atggtttcaa tcatatctgc tattggttta tttgacacaa tagggattct ccttcaagtt
    29161 aaaattcatt atagataaag cttccttgac cctgaccaag atagctaaag aagtaaagca
    29221 gccctagaaa gataagaaaa tacaaggctg tccgaccaag aaagaggtac aattcttttc
    29281 tctgtttcat caagaaaaac cattcatttc tgtaattttt cgctaaaata agagtgattc
    29341 ttactagctt atttttctac cattgtacca ctttatatag tatcttttca attgtttacc
    29401 gtatgtttcc aatagatttc agattatttt aaggattata cagtttttct atgtatattt
    29461 tcaaatagag tgatcctgct tcaaaactcc atttcaggag acaatgaagt aaatattccc
    29521 ataataaaac acacaatatc aagttttttc aacacctgat actatgcgct tttctgattt
    29581 ttaaagactt tttaaccact ctctcattta aaataatctc gtctgatata aattaaaata
    29641 gcttctatca tcagacaaat ggctgatagc caaaaactga tgctaatacc aaaactctca
    29701 gtaatatagc tcattagcaa aacaaatact gaaaatgcta atgtagaaat cacttcaaga
    29761 acggaataga cattaactaa atgattttcc tctactgttt cctgaagaaa tacactttca
    29821 ggaacttctt ttagttgcga taacatacca actaaagctg aaaataataa aaacatctgt
    29881 gcgtttggaa aatatagaat agtcagtgtc actattgcca tagctacaag aggaaaaaga
    29941 atactttccc atttagcagc aaggaacttt tcagatagcc taaaagcaat taagccacta
    30001 attataatac caatagagta tgctgtatta gaatatcccc agtaactttc cgttttattt
    30061 aataactccg ttacaaaaac aagtataatg gaagaaaccc aaatcgtatt tgaaaaaatt
    30121 tccaataaat ttgctgatac aaaaagtctt aatctaggat ttctagcaac taacttccaa
    30181 cctttgagca aaatttcaag attagtttct gactctaaca cctccacttc agcgttagga
    30241 agaaataaca tcagaaagct agaaatgata tacaagacta aaatgataaa cgtggtaggt
    30301 aacagaccaa ttgttgcaaa caagagtcca cctaatcccc aacctatcaa ttgaacagct
    30361 tcaccagtca ttgataaggc tgaattagcc ttacccaaat cggtcgcata gcgtggcaca
    30421 atagcatagg aaacgggtgc tgcaaaacca tctagtatgg aaattgcaac aacaaataga
    30481 taggtcacca aaggcgctac ggattgcatt acggtaaaca ttcctaccag tatcgccaat
    30541 aatatagtct ttccaaattg agataaagat aaaaccctat ttagcgctaa ccttttagta
    30601 accaacggaa ctaaaagact cgcaacaaaa gaggatattc ctattaagat aggaactagt
    30661 gatgtggcaa ttactgattt tgaaataatg tatatgttag caatgattgt tactctaaag
    30721 aaaatatctg ctaaatttgc aaataattga gagactaaca acttgacaaa tgaattagac
    30781 atgtagcgct ccccctttag ttacaaactt tggaattaaa tttgattttt aatattttag
    30841 cataaaatag cagaaaacaa aagtgaggtt ttttacatga aaaaacaaaa actattgcct
    30901 ttattattcc taattttagg aggaattatg attatcgttg ttggctagta ctaagaagtt
    30961 agttagttag tagaataatt acatcttcaa tcgaaaaaga gactggaaaa agtcttaaaa
    31021 atcagaaaaa cgctcaaatc ctttggcgct ctaagctttt ctaatgatag atttagatag
    31081 tcaaaaaaat atctgaaaag ttcccaggat cgtcgtctca gcatcaaaaa tacgtgaaat
    31141 ttgcataatt aaaataaatt tcaggggagt taatcgagta ggggataatc tctagcccct
    31201 ctcacaccac cgtacgtgcc gttcggcata cggcggttca actaactttt aacgcatgtc
    31261 gttcaaggta ataatccaaa cacgaaacca gtccacgttt ttccaggact ggttttgata
    31321 tagcacgttt aagtaccgac ttctgagcta ctaattgata atggtcgccc cagccagata
    31381 ccttatctgc tatccattta ggaactccta acttaagcaa tccccataat cgtctcgatt
    31441 tcttcttcca ttgcttccag ataatcactc gtaggcgagt acgcaagcgc tcatctatgc
    31501 tggcgactat acttttcata tttcccaatg agaaatagtt tatccatcct cgaatagaca
    31561 aattcagttg ctcaatacgt cttgttaggt ctatactcca tttcctctgt gttagtttct
    31621 tcaatttaaa cttaaatctc cgaacactat cttgatgtgg acggcttttc caaccatctg
    31681 atgatttcca gaacccaaaa cctagatatt tcaactctct tggtctggta atcttagtct
    31741 tggtcatgtt tactttcaaa cctagccgtt tctcaataaa acgactgact gaatacatca
    31801 cacgcttaga ggctgcctcg cttccgaccg taatcacaca atcatctgcg tagcgcacaa
    31861 atcgaagtcc cctcttttct aattccttgt ccaattcatt aagcatgata ttggataaga
    31921 gaggagataa atttcctccc tgtggtgtac caactagtgt tttataacgt tgaccattaa
    31981 tgataacacc tgaatgaaga tacttacgaa tcaaggattc cgtatctccg tcttcgataa
    32041 tgttatgtac taaggacatc aatctatctt gaggaactgt atcgaaaaat ttctctaggt
    32101 ctatatccac tatccactca tagccgtcat ttaagtattc taagagcttc atgatggctt
    32161 tttcacatga cctatttggt ctgaaaccat aactcgtatc tgagaaatgg ggttcacaaa
    32221 tggggctcat gacttggaca atggcctgtt gaatcattct atccataact gttggaattc
    32281 ctagttgacg gatgcctccg tctggtttag gtatctcaac tttaagaact ggttgaggct
    32341 tatattttct ctgttttatc agttccttag tcaagcgcca gttttgtctg agataattat
    32401 ccatctcttc gatagtcatt ccatcaatcc cagctgagcc tttattggat tttacttgat
    32461 tgtaggcttc cagcatattt tcgcgtgata atatcttatc tagcagtttt gacatgtgcg
    32521 tattcctttc ttgttttggt gcctgaggga catcttatat tggtgaacct tcatctagtc
    32581 attacgtgtc aaagtccagt tctatcagac cactcatttt acagacacaa gtgaagtagg
    32641 attacttcga ttagttgttc accccttcgc tccacttcca ttacggaagt ttcctcacta
    32701 ctatgggctc ggctgacttc tcatgattcg ttgttactac gctttcagcg ctcatgaaac
    32761 ctcacgggat aagtcgtaca tctttcctcg tttatacaag atatgaggac gttaaaaggt
    32821 tacagtgaaa ataggaaatc tgacgcagag tctttagact ctagaaagat ttatcttttt
    32881 cacacaaacc ttagtccgaa ttcaattact ctcgtgattt acacatatag gttacgacta
    32941 cctttttgga ctttagagtt tgaagcctcc ttatccgcta tatacgcctt actatcacgt
    33001 tcctgttcgt agagccacga tttcgctatc ccttcctctc cctgctacct cacgatagtc
    33061 aggcttggga atcgctattg ggttcaccgg tagcaggtgc ccaccgagga cttacacctc
    33121 agatatacga cat
//
LOCUS       DAVKLX010000023        32645 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKLX010000023 DAVKLX010000000
VERSION     DAVKLX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32645
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.023"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(332..1021)
                     /gene="rplA"
                     /locus_tag="VW325_001105"
     CDS             complement(332..1021)
                     /gene="rplA"
                     /locus_tag="VW325_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9734519.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1229..1654)
                     /gene="rplK"
                     /locus_tag="VW325_001106"
     CDS             complement(1229..1654)
                     /gene="rplK"
                     /locus_tag="VW325_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9734520.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            complement(1777..2493)
                     /locus_tag="VW325_001107"
     CDS             complement(1777..2493)
                     /locus_tag="VW325_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9734521.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(2511..2972)
                     /locus_tag="VW325_001108"
     CDS             complement(2511..2972)
                     /locus_tag="VW325_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9734522.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            complement(2974..4011)
                     /locus_tag="VW325_001109"
     CDS             complement(2974..4011)
                     /locus_tag="VW325_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9734523.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDTRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            complement(4109..5434)
                     /gene="brnQ"
                     /locus_tag="VW325_001110"
     CDS             complement(4109..5434)
                     /gene="brnQ"
                     /locus_tag="VW325_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9734524.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5605..5697)
                     /locus_tag="VW325_001111"
     CDS             complement(5605..5697)
                     /locus_tag="VW325_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9734525.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(5740..5892)
                     /locus_tag="VW325_001112"
     CDS             complement(5740..5892)
                     /locus_tag="VW325_001112"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9734526.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            complement(6052..6630)
                     /locus_tag="VW325_001113"
     CDS             complement(6052..6630)
                     /locus_tag="VW325_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9734527.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(6684..8084)
                     /gene="pepV"
                     /locus_tag="VW325_001114"
     CDS             complement(6684..8084)
                     /gene="pepV"
                     /locus_tag="VW325_001114"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9734528.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(8097..8702)
                     /locus_tag="VW325_001115"
     CDS             complement(8097..8702)
                     /locus_tag="VW325_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9734529.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            8873..9673
                     /locus_tag="VW325_001116"
     CDS             8873..9673
                     /locus_tag="VW325_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9734530.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(9857..10690)
                     /locus_tag="VW325_001117"
                     /pseudo
     CDS             complement(9857..10690)
                     /locus_tag="VW325_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(10680..12527)
                     /gene="uvrC"
                     /locus_tag="VW325_001118"
     CDS             complement(10680..12527)
                     /gene="uvrC"
                     /locus_tag="VW325_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9734531.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEIYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(12567..13268)
                     /locus_tag="VW325_001119"
     CDS             complement(12567..13268)
                     /locus_tag="VW325_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9734532.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13347..14579)
                     /gene="murN"
                     /locus_tag="VW325_001120"
     CDS             complement(13347..14579)
                     /gene="murN"
                     /locus_tag="VW325_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9734533.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(14584..15804)
                     /gene="murM"
                     /locus_tag="VW325_001121"
     CDS             complement(14584..15804)
                     /gene="murM"
                     /locus_tag="VW325_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9734534.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(15916..16695)
                     /locus_tag="VW325_001122"
     CDS             complement(15916..16695)
                     /locus_tag="VW325_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9734535.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYMALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(16764..18425)
                     /locus_tag="VW325_001123"
     CDS             complement(16764..18425)
                     /locus_tag="VW325_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9734536.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(18600..20822)
                     /gene="recJ"
                     /locus_tag="VW325_001124"
     CDS             complement(18600..20822)
                     /gene="recJ"
                     /locus_tag="VW325_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9734537.1"
                     /translation="MITPTYEWQFALQVENADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSIPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQILEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEVSFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMMDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21099..21858
                     /locus_tag="VW325_001125"
                     /pseudo
     CDS             21099..21858
                     /locus_tag="VW325_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            21898..22662
                     /locus_tag="VW325_001126"
     CDS             21898..22662
                     /locus_tag="VW325_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9734538.1"
                     /translation="MISLFGLVAAKPVQANTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLTFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            22675..23352
                     /locus_tag="VW325_001127"
     CDS             22675..23352
                     /locus_tag="VW325_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9734539.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFCIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23362..24021
                     /locus_tag="VW325_001128"
     CDS             23362..24021
                     /locus_tag="VW325_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9734540.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKIKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVPVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            24317..25507
                     /locus_tag="VW325_001129"
     CDS             24317..25507
                     /locus_tag="VW325_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9734541.1"
                     /translation="MKSIKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKRVHFYYSFRGEENAVYLDLLRDYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCSPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(25828..26709)
                     /locus_tag="VW325_001130"
     CDS             complement(25828..26709)
                     /locus_tag="VW325_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9734542.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(26850..28178)
                     /gene="vncS"
                     /locus_tag="VW325_001131"
     CDS             complement(26850..28178)
                     /gene="vncS"
                     /locus_tag="VW325_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9734543.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(28175..28831)
                     /gene="vncR"
                     /locus_tag="VW325_001132"
     CDS             complement(28175..28831)
                     /gene="vncR"
                     /locus_tag="VW325_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9734544.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(28880..28963)
                     /gene="pep27"
                     /locus_tag="VW325_001133"
     CDS             complement(28880..28963)
                     /gene="pep27"
                     /locus_tag="VW325_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9734545.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(29143..30522)
                     /gene="vex3"
                     /locus_tag="VW325_001134"
     CDS             complement(29143..30522)
                     /gene="vex3"
                     /locus_tag="VW325_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9734546.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(30574..31221)
                     /gene="vex2"
                     /locus_tag="VW325_001135"
     CDS             complement(30574..31221)
                     /gene="vex2"
                     /locus_tag="VW325_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9734547.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(31234..32511)
                     /locus_tag="VW325_001136"
     CDS             complement(31234..32511)
                     /locus_tag="VW325_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734548.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9221 a         7487 c         6073 g         9864 t
ORIGIN      
        1 ctaaaacatt gttagaaatc gatttgactg tcctgaacga ttcgtcctat tcttatttca
       61 ttttattata attgatagtg gtcgccccag ccagatacct tatccactat ataggcctta
      121 ttatcacgtt cctgttcgta gagccacgat ttcgctatcc cttcctctga cgattcttat
      181 ttccagcgtc agacttggga atcgctattg ggttcaccgg tagtaggtgc ccaccgagga
      241 cttacacctc agatatacga catgcccgtc gtacatcaaa aaaagttgag acatagtgtc
      301 ttcaactttt ttacattact ttaactactg attaaagtga gtttacgtca actttgatac
      361 caacaccttg agtagttgtg atagtcaagt ttgttacgta agttccttta gctgtagctg
      421 gttttacttt ttggattgtt tcgttgaaag ctttaaagtt ttcaaccaat ttttcagctt
      481 caaatgatac tttaccgatg attgcttgaa cgttacctgc acggtcagca cggtaagtga
      541 ttttaccacc tttagactct tcaactgctt tagcaacatc cattgttaca gtaccagttt
      601 tagggtttgg catcaagtta cgtggtccaa ggacacgtcc aagacgtcca acaagagcca
      661 tcatatcagg tgtagcgata actacgtcga agtccaacca accgtcgttg attttagcaa
      721 caaggtcatc ttcaccaaca aagtctgcac cagcagcttt tgcttcttca gcttttgcac
      781 cacgtgcgaa aacaagaaca cgtgaagttt taccagtacc gtttggcaat accattgctc
      841 cacggatttg ttggtcagct tttttaacgt cgatgttcaa gttgtaagca acttctacag
      901 ttgcatcaaa ttttgcaaag ttagtttctt ttgcaagtgc tacagcttct tctacactgt
      961 atgcttttgt gctgtcgatt ttctcaagag cagcacgaag ttgtttgctt tttttagcca
     1021 ttttctattc tccttgtaag tggttcaatc gattttcatc tcccacgtca cttctcgaaa
     1081 tgatgaagtc ttgcgggttt tcagtctact atttttctct tcctacggcc ttaagctccc
     1141 ttgctgtatc caagtacaag cctcagtcac ttgcctagta tgaaaagcaa ctaatagact
     1201 gctgtgagat tttctactgt gttattgatt agtcaacaac agtgaatccc atagaacgag
     1261 cagtaccttc gatcatacgc attgcagact ctacgtttgc tgcgttcaaa tctggcatct
     1321 tagtttctgc aatttcttgt acttgtgcac gagtaactgt agcaacttta gttttattag
     1381 gtgtacctga tcctttttca acacctgcag cttttttcaa aagaacagca gctggtggtg
     1441 ttttcgtaac gaaagtaaat gatttatctt cgtaaactga gataacaact ggaatgatca
     1501 taccagcttg gtcagctgta cgagcgttga actcttttgt gaatcccatg atgttgatac
     1561 cagcttgacc aagagcaggt ccaaccggtg gagctggtgt agctttacca gcagggattt
     1621 gcaattttac aagtttttcg acttttttag ccatttttaa atcctccttt gtggttttgg
     1681 cggtaattag agatttttac ctcccacaag tatgctttac acataccttt ccattataca
     1741 ctatttatct aaaaatgcaa gagaaaactt tcatttttaa tcgacataat cgccgccttc
     1801 aaagccatag tactcttcca aactgagacc acttgcagca atttctttgg cttcttttcc
     1861 aacgtagcgg agatgccatt cttctgccat gtagccagtc tccttttcct tgcctttaag
     1921 atagcgaaca acaaagccat agtcagccgc atggtccaag agccactggg ctgccttttc
     1981 ttcagtaact aaatccccat ctgtcccaat caaatcaaaa gcaagacccg tctggtgttc
     2041 actgtaacct gggcgtgctg agtagcgatc tgctgcttcc ttaccatctt gattgacata
     2101 gtcttggtag agcttagcct gagtttcata actcctaaac ccactgtatt ggtcactgat
     2161 tggatagccc tcagcctgca tagctgcgat tagtttgagc aactctgcct tggctgttgg
     2221 attttctcct ggattgtaat cttttgacaa aggataacgt ttattagcta ggataatctc
     2281 atcgtatttc ccttggatac tataatagct ccctttattg ctaacttctg cctttttctg
     2341 actagaagct tgagatttgc tacctacagt tccttccggt tgactgcttt gctccgtttt
     2401 ttgagtgttt tcttcaactt tggttttttc ttgcgaacaa gctgcaaggg tcaatgctag
     2461 taaaccagat aaaatgacat atcttttttt catttctttc ttcctctctc ttaggccaaa
     2521 tacttctcca attcatgaga taagatttta atcatctctt ccagctctgg cgattgtact
     2581 tggcaatctt ctgccgccat ttcttcccag ggcacccacc agactgggcc acggccattc
     2641 aaaccgtgac tcttcatatc acctgctctt cggggaaaaa gatgccaatg agcgtgggca
     2701 tcgccatttc ctaggagttc aatattcatc ttttcagcct caaatgcttt ggcgactgct
     2761 tcttggacta aactcatttc ctctaaaaag cgaagtttta cagaagtttc catatgatgg
     2821 agttctgtga catgatcttt tgctaaaaat aaggtatagc ccttaaagta ttggtagtca
     2881 ccaataacaa catagcctgt ttctagttct tttacaaagt agggattttg cccagcttta
     2941 atgagttcga ttctctgaca gattaagcac attctatgat accgcctcgc tcttaagata
     3001 agcatcaacc atacgctcag tcgctattac tgaatcgatg tgggtgcgct cataagagtg
     3061 actggactca ataccagctc caagaagtgc atgcttgacc tctgcacctg ctgacatcgc
     3121 tgctgaagcg tccgatccgt aaaatggata gatgtcgagc ttaaatggaa tatcttgctc
     3181 tttagccaag gcaaccaagt gttgacggaa gtcatagtga taaggacctg aagcatcctt
     3241 gacacagata gatactgtgt actcatctgt ctgctggtca tctcccatag ctcccatatc
     3301 cacagccaga tattctacta cctgagcagg aatgttagaa ttagcaccat gtcctacttc
     3361 ttcaaaaact gaaaaagcaa aatgagttgt tactggcaat tcaatcttct ctttcttata
     3421 aatacgaagc aggttgagca aaatcgctgc gctgacctta tcatccaaat gacgagactt
     3481 gataaaacct gtatcagtca caacagttcg tgtatcgaag ctgataaaat caccgacctc
     3541 aatcccaaga gcacgagttt ctttttcatt gcttactttt acatccaaac gcacttccat
     3601 attgtcctgc gtgcgttctg cagttcctgc atccttgtag acatggcaag aagtttggtg
     3661 gatgaggatg gttcctgata ccttttgacc tgtgctagcc acatgaacgg tacagttttc
     3721 accttcaatc atgttccaag gaaaaccacc gatacggtcc aatttaagac gaccatctgg
     3781 tttgacagca cgaacaatag cacccagcgt atccacatgg gcagtcacat agcgatgttg
     3841 ctcatcattt tgacctttga tggtcacatt gacaccaccc ttagctgtgc gaactggctg
     3901 gtaaccaaaa ccttctagag tcttgactaa ataattcgaa atttcacgag tgaagcccgt
     3961 tggcgatgcg atggctgtca gttctttgat atattctact gtttgattca tttcttcacc
     4021 tctttcgcta cctattataa cacatttatc atcggataaa aaaagaaaat cactcctgta
     4081 gacttggcta caaaagtgat ttaagtgatt attccatttc aaaaacatcg ctttcttgct
     4141 tgtttggtag aaccaatgag agcaagattc caacaatggc tggcaccaac catgggagag
     4201 atgccttggc aaaaggaaga acgctaacaa gatttgcaag aaactcaacc ttaaacgagc
     4261 ttcctagtac gcttgcaatg gcaataactg taaccacagc aattgtcaac tgcatacctg
     4321 gttttgaaag ggcaacaaat ttgttgacaa tgacaatcat aacgatagca atcgtgattg
     4381 ggtacaagat aaccagtact ggaattgagt acttgataat cgcatcaaga cccaaattgg
     4441 caatagcaaa tccaatcaag gtaaaggctg tcgcataaac cttgtagctg atttgtggga
     4501 agcgctcatt aaagaactca gctgttgaca caatcaaacc aacagtcgtt gtgaagcagg
     4561 ttacggtaac catagctgca aggaagagtt gagctgttga gccaaagatt tcttgagtgg
     4621 cttgtgacaa gatgtaaaca cctggtgttc cacccttcat cgcttcagct ggtactggga
     4681 aatgatttcc aagaaaacct aaaccgatgt aaagagcgct gaaggcaagg gcaacaacga
     4741 taccaacaac ccaaatagtt gaaatgtatt ctttcttact tgaaaatcca agttgtttca
     4801 aggtttgaac tgcgattacg ctaaaggcca ctgaagcaag ggcgtccaag gtattgtaac
     4861 cttctaggaa acctgtacca aaggcagaag cttgataagc agctgaagca gcttgaggac
     4921 ttgttccacc atatttgata gctcccagaa cgaccaagat aacaatcaaa attgcaaaga
     4981 ctggcgttaa aacacgtcca atgcggtcta agatttttga tggattaagc gaaatcaaat
     5041 aggctgccgc aaaatacaga accgtaaata caatcaagcc aagtccttta tttgcatccg
     5101 acaaaagggg gctaatccct acttcgtaag ctgttgtagc agtacgtggg gtagcaaaga
     5161 atggaccgat tgacaagtaa agaactgaga ggtaaagagt cgcaaaccaa ggcgctatct
     5221 tcgttgaaat ctcgtagata tatcctttag gatttagcgt tccaataata agggtcaaga
     5281 cggcgatacc aacgcctgaa aagacaaaac ctgcgatggc aggaagaaaa tgttctccag
     5341 atagagcacc tagagaaggc ggaaaaatca agttccccgc accaaaaaat attccaaaca
     5401 ggagcaaacc tgttagggta ccttttttag ccataaaaat ctccttcata tttataaaat
     5461 actgacctag tataccacga ttaggagtct gaaaaaagca tcacgaggca aatattaaat
     5521 gttttcaatt ttcttaaaaa taagaattat ctaaaaataa aactctccta cccagtccac
     5581 accaagtagg agaaatgcaa aattttaaag ttcttcaaag gctaacatgc caccctggac
     5641 attgataaca ttataacctt gttctaacag gaattggcaa gcacgcgccg atctcattcc
     5701 agatttgcaa ataatataat gcaagcgatc tttgtccaat taatcataac tatcagccaa
     5761 ttgactaagc ggtaggttgt gggcacattc taaatgaaga gctgcaaact catccacttc
     5821 tctcacatcc actagagaaa gctggtcgtt ttggtaaagc tggtaaaatg cgtcaaaggc
     5881 tatttctttc attctttttc ttctaatact cttcgaaaat ctcttcaaac cacgtcagcg
     5941 tcgccttacc gtgcgtatgt taccgacttc gtcagtttta tctgcaacct caaagctgta
     6001 ctttgagcag cctacgacta gcttcctagt ttgctctttg attttcattg actataaaat
     6061 ggttttaatt ctttttttca aatctggcac tacttctgcc tcaaaccaag gatttttggc
     6121 catccagatt tgatttcgtg gtgatgggtg aactagcgga aaataggctg gcagatagtc
     6181 tttatagtgt ttcaccctct ccgttacctt cccactgatt ttctcctgta aatagtaggc
     6241 ttgggcatat tgcccaatca agagggttaa ctgaatatca ggcaattcct gtaagagctg
     6301 cggatgccat ttttctgcaa aacctgtacg aggcggaaga tcacccgact tgccatgtcc
     6361 tggaaagtag aaatccatag gcaaaacagc aaaataacct gaattgtaaa aggtatcttc
     6421 atccacacct agccagtccc gcaagcggtc accactttta tctttccagt aaaggcctgc
     6481 ttcttgagtt ttaagtcccg gagcctgacc gatgatattg atgcgagcag tttttggcgc
     6541 tgcaaagaga ggctcaatgc cacgctctgt atagctggca ttctgtgaat ccgccatgat
     6601 agcctgtttg attctttcga tttgagacat ctactttccc tccaaaaaga agtccaagca
     6661 taagatctca gacttctatc gttttatttg atcaattcgt aaatagcttc ggcatagatt
     6721 gctgctgctc ggaagagatc gtccaaggcg ataaattcat tggcttggtg catggtatca
     6781 attgagtctg ggaacatagc accataggca actccgcgct ctaacaagcg accaaaggtt
     6841 ccaccaccga tgacttgttc atgaccttta aagccagttt gtttttcata gatattcaac
     6901 aaggtttgca caagtggatc ttccattggc acatagtgag gcgtgtgacc gtgttcagac
     6961 aggctaacag aaacaactgg caagttttca aggattgact tgatttgttc tggacttgtt
     7021 ccttttggat agcggatgtt gagggcaatg gtattatcag cacttgtttc atcgaagcgg
     7081 aagacgccgg cattcataga aagagcaccc atcttttcat ccacatgagc aatcttaaga
     7141 ttttcaccct catgatcgtt caagagaatt ttacctgcga tgtcaaggta gtctttggct
     7201 ggaccagcaa agccaaactg actgaggaag agggcaaggt aagttgcgcc attgacacct
     7261 gaagcaggca tagcaccgtg ggctgattta ccaatgatcg tcaccttgta tttgccagct
     7321 tcttcttgga gttctcctct aagtttgtgt tctgcaacaa aggcatctag tttagcttgc
     7381 aagtcagcca agtcacctga aacgactgct gttgctgatt ctggtaccat attttcacgt
     7441 aaaccacctg taaagctgtg aagacgggca acacctgtat tttctcctgc aaagtggagg
     7501 tattccgtga tatttccttt ttcaccattg atgattggaa attcagcatc tggtgagaaa
     7561 ccgaaatctg gtttggcaag tcctacgtgc tcaaagtagt agtccatgtc tgcccagcct
     7621 gattcttcgt ctgttccaac gatgaagcga actttcttag aagttggaag acccaattct
     7681 ttgatgattt tcaaaccata gtaacaagct gttgtaggac ccttatcgtc cgaagccccg
     7741 cgcgcataaa ggcgaccatc tttgatagtt ggtgtgtaag ggtctgtgtc ccaaccgcta
     7801 ccagcaggca ccacatccat atgggcaaag attccgagaa cttcttctcc atcaccaaac
     7861 tcaaaatgtc ctgcatagtt atcaacattc ttagttgggt agccatcgcg gtctgcgatt
     7921 tcaaggaatt tctccaaggc ttttactgga ccaggcccaa atggatgctg ggcatcagcc
     7981 ttgctgtcat cacgttctga attgatttcc aaaaggctaa acaagtcagc caagaggtct
     8041 tctttgcgtt tttctacttc tgctgtaaaa tcaattgctg tcattttttc ttctttctat
     8101 cttttctcga tgatttcatc tactggcaag cggtagcttg gttccaattt ttcgtctgta
     8161 taacccactg tgatcaagag ttctgggcgg aaacggtctt cgatttccaa aacttcatta
     8221 acttttgatt tgtcaaaacc aagaataatg ttagaaccaa ttccttggtc tgtcaatgca
     8281 agaaccaagt tcatggcaac caaacctgca ttgagagcta ggtagtcgct gacttgttgt
     8341 tcattgtagc gtgcaaattc agctggcaaa tttttcatga agtattgaag ttgttcttct
     8401 gagaaattgt tagcaccacc aactcgggca atcttacgag cgcgtttggc aaggtctgta
     8461 tctgtaaata gggcaatggt tacaggtgct gatgatacct gctcaaagtt tgaaccataa
     8521 gccaactttg ccagttcagc atttttctca cgaacaacta caaatttcca aggctggctg
     8581 ttgtgggcgc ttggcgccaa ggttgcaatt tcgatagccg tacgcacatc ttttggatca
     8641 acaggcttat cagtgaaatg cttggtcgca tgacgttttt tatttaattc aagaaatttc
     8701 ataatcgatt tccttttcta attctactgc ttccattcta ccataatttg aaagagcttg
     8761 cagggatttt tcatgattaa gtttttctat cacagacata aaagatatat attggtttat
     8821 caggaaagtt tctgataaac tagcaagtac tttacatttg aatggagaca gtatgaaaaa
     8881 atttagccta ttactagcta tcctaccatt tttggttgcc tgtgggaatc aagctacacc
     8941 caaagagact agcgctcaaa agacaatcgt ccttgctaca gctggcgacg tgccaccatt
     9001 tgactacgaa gataaaggca atctgacagg cttcgatatc gaagttctaa aggcagtaga
     9061 tgaaaaactc agcgactacg agattcaatt tcaaagaacc gcctgggaaa gtatcttccc
     9121 aggacttgat tctggtcact atcaggctgc ggccaataat ttgagttaca caaaagagcg
     9181 tgctgaaaaa tacctttact cgcttccgat ttccaacaat cccctcgtcc ttgtcagcaa
     9241 caagaaaaat cctttgactt ctcttgacca gatcgctggt aaaacaacac aagaggatac
     9301 cggaacttct aacgctcaat tcatcaataa ctggaatcag aaacacactg ataatcccgc
     9361 tacaattaat ttttctggtg aggatattgg taaacgaatc ctagaccttg ctaacggaga
     9421 gtttgatttc ctagtttttg acaaggtatc cgttcaaaag attatcaagg accgtggttt
     9481 agacctctca gtcgttgatt taccttctgc agatagcccc agcaattata tcattttctc
     9541 aagcgaccaa aaagagttta aagagcaatt tgataaagcg ctcaaagaac tctatcaaga
     9601 cggaaccctt gaaaaactca gcaataccta tctaggtggt tcttacctcc cagatcaatc
     9661 tcagttacaa taacactctt cgaaaatctc ttcaaaccac gtcagcttta tctgcaacct
     9721 caaagctgtg ctttgagcaa cctacggcta gcttcctagt ttgctctttg attttcatat
     9781 tgagtataag atatattaaa agcgatggga tcagccaatc gcttttttag tttgcattgg
     9841 ttattttagg aaactcttac aaaaaatcaa aaaaattctt cacatcctct acatacctat
     9901 gcttttctat taagcttgct aatagttcca gattgtgcct ctgatagtta tcttcacgac
     9961 gtagttcttc atacatttcg ataaagggaa aacccttgga tttggcaaat tctaactcag
    10021 cctcgtaatc ataagcgact ttacgaaata ggatatttac caattcccca tcttcaacct
    10081 ctatcacggc atactgggca cggtgatttt ttaacgcctc ccaattaaaa tagggcatgc
    10141 caatcgaccc tggattgatg atttgttgcc cttgactacc ataacgaagc aactacttgt
    10201 gaacatgacc ataaactgcc acgtccgttt ccgcatctag cagttggtca aatttctctg
    10261 tatcattctc aactagcaag tcaccaccat agtttttgtc aggtaaatta tgagagatag
    10321 aaaagcgcaa tccgtcaatt tctttctttt ccagcaaagg caagcttcgt agccagacaa
    10381 tcgttgcagg atccattcgc tccatcaaat actgtgtcat acgcaagagc tgaacttcct
    10441 gtgggtcttc taagccatat tgcccatcta aagcctcaag gacacaatca tcccaattgc
    10501 ctcgaacact tgccgtgata ggaagttcct ttaacagagc gaccaagtca tttgcacctg
    10561 gaccagggag gaaaatgtct cccatgagcc aatattcact ggctccttga gttttagcat
    10621 ctgcaatcac tgcttctagc gccgtcgcat tgccatgaac atctgataaa attgcgattt
    10681 tatggtttat tgtggtttcc ttccgtttgg taatctactc tttcttccgc cacttgaagc
    10741 aagatttctg tttcctgcgg gttcaacttg gtttgcacag cctctgctac tgctcttggt
    10801 actccaactt cgacaatctc atccacactg gcttccttga ttttggtcag agacttgaag
    10861 tgcctcatga gattttgctt gcgtttaggt cccagaccat caatcccatc caattgagat
    10921 gagaaagaat ttttggagcg cagttggcgg tggaaggtaa tggcaaagcg gtggacctca
    10981 tcttgaatgc gttggaggag gaaaaattcc tgagaattgc gagacaagtc caccacttcc
    11041 agcggatctc caaagagcaa ttcatgggtt tggtgcttat cattcttttg cagacctgca
    11101 attggaatat ccaagcccag ttcttcttgg atgacttgct tagcaatatt gacttgacct
    11161 tgccccccat caatcacaat caaatctgga ggagtcaaag cctcacgctg tactcgacca
    11221 tagcgtctgc gaatgacctc tcgcatactg gcatagtcgt ctggcccgac tacggtcttg
    11281 attttatact tacggtaatc cttcttacta ggcttgccgt tgacaaagac caccatggct
    11341 gaaacaggac tagttcccat gatgttggag ttatcgaagg actcgatgcg aactggggtt
    11401 gggatttgaa gcaagcgtcc tagattttca atagctcctt gggtcttctc gacagatttc
    11461 tctagcaggt tgaacttctg ctctaggcta acacgggcat tttttatagc taaattgacc
    11521 agctgctttt tctctccacg ttgaggctta agaatcttgg aatccaccaa ggccttgaca
    11581 gcctcttcgt caatatcttg cggaatcagt acctcattgg gaactagatg ggatttttct
    11641 tgatagaatt gtcctacata ggtcaaaaaa tcctcatctg gatcattgaa gtaggggaag
    11701 agattgacat cgcgctcgat gagcttaccc tgacggacaa agaaaacctg cacacacatc
    11761 cagcccttat ccacatagta gccaaagaca tcgcgatttt ggagatcctt agccatgacc
    11821 cgttgcttgg ttcgaagcgt tccaatagcc tgaatcaggt cacggtattc cgccgcacgt
    11881 tcaaactcca tagtctgggc tgccgctgcc attttgccct ttagctcatc aatgattttg
    11941 ttatcctgcc ctttcagaaa atcagaaacc tcctgggcca tagacttgaa ataaatctca
    12001 tccttcttac agatggtgtg ggccatgcat tgaccgatat ggtaataaaa acagacctta
    12061 gagggcgggt tggtacactt acgaaaaggg aatatccgat ccagcaaccg cttgatttca
    12121 ttggctgccc ccacatcggg atagggtcca aaataaagac ctccgtcctt tttgacctga
    12181 cgagtgataa tcaagcgtgg atagcgctca ttggtgattt tgatgaaagg ataggacttg
    12241 tcatccttga gcatgatatt gtacttgggc ttgttttcct tgatcaggtt gatttctagg
    12301 agaagtgcct caatattaga ctccgtaaca ataaattcaa aatccacaat ttcagacacc
    12361 agagcctctg tcttggtatc atgacttcca cgaaaatagg accgtactcg attacgcaga
    12421 tttttagcct ttcctacata gataatggtg ccatttttat ccttatgaat gtagcaacca
    12481 gggctggtcg gcaagagctc tagttttgat ttaatcaagt tgttcatagt tccattatag
    12541 caaaaaaagt agggaaagat agtaccctac tcactagtct catatatttt tcggaggttt
    12601 gcaacatcct cttcctggat accataaaag gaacctgctg gttgcatgac agacttctca
    12661 tctgtatggt ccaagccaat cgcatgacct aactcatgtt ctgccgtatg gacaaggcgt
    12721 tcataggagt agccatagta tggattggac aaataataat gattcaaccg caccgttacg
    12781 gacaagaatt gccctgttaa gagattagtt tgactttccg cctctcctgc cacaggagtg
    12841 cctccgtcgt tcatctccgt agccgtaata tccgccttac tagactcagt cacgagttca
    12901 aagttaaaag caccagtttg attccagttc tggattgctt ctaaataagc ctcttgaaag
    12961 cgtgaatcca tctgcggatc caggtaaata cgagcagagg cctgtgccca ccttccttca
    13021 gaatgctggt gactatctgt ctggcttaac ttaggcagtt gccctgtttt ctcccattgg
    13081 tcaatacttt gttgaccaat ttttactgac cgttctgctt gctttagcgc tccttgaaaa
    13141 tctccgttca gataccagag aagtccgaaa gcaagcacac ataagagcac aactatccaa
    13201 accagacgcc aaaacaaacg ccacacaaaa gaaaagaaag cccctatcaa acgaaaaagc
    13261 cagcgcatgt ctctccacct ttctagcaaa taagaattat tttactaaaa gaagctaaaa
    13321 gaaatctaaa tactgacttt ttcatcttaa cgtcctacta tttttttgag taactggata
    13381 gctttgtatt ttaaaggaga tggatggtaa cggaaagtac ctgctttgcg tacaatatag
    13441 ccattaaaat tctgtttaaa acgcaaaaca ccatcacttc catcaaaaat cccttgaatg
    13501 cctaggaagt tgtatttagg tattccacgt tttatgcttt ccaacataac atatttttga
    13561 agcagggcag gggcatagaa cttattaaac tcagtgtagg aaccactaaa gagataagtc
    13621 gtttcctgag gcatataaac aaataaactc ccagctaaaa caatatcttc ttctccatat
    13681 ttttcaatca agtctcgcgc ttctgctttt cgaacttcaa acgtttcaaa ttgactagaa
    13741 tattctctca gttgattttg ttttttctca gaatgaggat ttttactcaa atcaagtcgc
    13801 aacttgtcca agttttcttc tagtttactt tgttcacctt gcaatttgct catatagtcc
    13861 gaaaaattta agcttgctat gagaaactcc gcttgttctc caaaagtatc ataaaaatgc
    13921 tcataatatt ctaaactttt atcactatat tctctacgtt cagaggtttc ttttgttata
    13981 ttcttaaaaa tcgatagttc ttcacgtttt aactttttca accgaatgcc aaaggtttca
    14041 gcctttttca ccaagggttt accctttttg ctaaaacttt taagcaaact cttttcagtt
    14101 aattcagtta aatctttata gtataaccaa tctggttctc cacctgggta acctgttgtt
    14161 aagccatcaa attgataacc taaatcagtc aaaccttgaa taatactttt tttctcagca
    14221 tctattggat taccttggct atcaaaagtt tgataagttt cataaggttt tacaagcaac
    14281 tctaatacac cattttgctt ggcatattct tttaactctg cataaaaaac tggaagagca
    14341 tcttgttggg tataaattgg ccccgaattg agttccatat gcagaccacc cagcatgggc
    14401 aagctataaa ccagagctgc aacttgaatt tctccttctt gtttcaaagc aagataaaca
    14461 attcgagccc ctcttttttc tagcaaatcc cccatctgga cagattgcat aaaggaacga
    14521 gaagaaacct gatcagaata agtctgaaac tcttctttcg tgagtgttgt tagtgccata
    14581 tacttacttt ctatgttttt ttcttaatgt tttacggaaa tcaagagcaa gtcttaacag
    14641 aggatagaga ggatgagtgg gcattgtaaa ttcacccaag tattcttcaa tcgttggatt
    14701 aaatttttcc ttaaaatgat aaagtccacc attgagagag ttttcaacac cacctaaatt
    14761 ttgccagacc atacctcgct caaaggcata gcgagccgtt tcataccatg ttaaaattgg
    14821 tgcattgtaa cgtttaaaat catcatccat accagcatat atattaacag aggtagtacc
    14881 aaattccaaa ctcaaagtag ccgctaaagg aactctcgct tgacctacat ctatatattc
    14941 ctgcaagaag gtcaattcct ctaacaaacg ttctttttcc ttcttctgcg cttctacttt
    15001 tgaagttcga gtcgactcag taaacgtctc ttccaaggct ctatttttcg ctaactgttc
    15061 ttctaattct tgcgaacgtt tagaaacatc caaggtggcc aaggtgatat aggccttgtc
    15121 cttaaaatta tctaacaatt ttttataata ggcttcattc ctcaaatgaa tctcttttcg
    15181 cttctcagtt tttttcatca actccgaaaa tgaatctaat agttccagtc caccatattg
    15241 aatctcaagc cctttgtttc gtgctgttcg aatagcctgt tttgttgact tggaaagttt
    15301 atcttcttca aaattttcct tgtatatttt cgcctgaata cgaggttgaa tggtgtctcc
    15361 catttcctcc gtttttcctg accaccttac tcccatttgt tgcaaactat caataatagc
    15421 cagattttca ggaaattctg tcttttcctg attgattaaa ctttgagata ggcaaatact
    15481 tgggtcaaaa gtcacaaaaa ccgctctctt actgcgagca taggacttaa tagactgaat
    15541 ggcaaaattc aagagttctt tatccccata atccaatata ggtcctcttg ggatgtaaaa
    15601 cattttatag cctagcggaa gagttctaat caaaatacta gctgtcgcca gtaatttttc
    15661 ttccctgtaa acaccaaact tctcatgttg ccaattagac ttaacttcct cccaagcact
    15721 actttgtaat acattggcta attcatgttc tttgacaaac tgatcatatt ctaatgtggg
    15781 aatgccaatt tgataacggt acatttccta ctctctttcc tccagtattt tattactatt
    15841 ctactacttt cgtttgaaaa gtctagtgaa aacatatttt attttagttc tactccccct
    15901 atgctgaagt tcaaactaag tcagtctctc ttctaatttg aaatcaattg gtagggttgt
    15961 taaaaaaact tccaattgtt tttcccagta gtaccactcg tgagttccag cactatggct
    16021 ataggtcaca tcaaaaccta gttttttgag atttttcact gcgagattat tggcttcgta
    16081 caagaaatcc tgttcgccac accacgccca aagtttggtc tttttatccg attttttagc
    16141 cagactttca agagaatagg gactagttgt ccagtctcta atctctccaa aaacacctct
    16201 ccagtaggct ggacttccca gattttgact ttcaggagaa aagttttgaa agctgagggc
    16261 acctgaaaaa ctagctgcat gagaaaaacg atttgtcgta agagccagtt tgaagcagcc
    16321 gtagcctccc atagaaagac cagcgataaa ggtcttttca cgcttgctag tcatattagg
    16381 gaagaagcgt ttcagaacct gtggcaattc ctctgctaga gccatgtagt agtcaaaacc
    16441 atactgggta tcggtgtacc aaccattgct ggtattgggc ataacaacga tgagattagt
    16501 tcctcgaagc aagcgttcta cattggtccg cttaagccaa ctattatgat ttccagacat
    16561 cccgtgcaaa aggtacaaga cgggaatatc ttcacactct ggttcttcca ctcgattggc
    16621 atcagggtag aggacattca ccccccactc catatccaat acttgtgagt aatactcgat
    16681 tttcattact gccatgattt tctccttcta tttttctata agaaaaagcc gaaactcgac
    16741 tttctctctg tttatttcaa cgattatttt gcttccatga ctactggtaa aatagctgga
    16801 cgacgcttgg tttggtcaaa gagatacttg gtcaaattat ctcgaacctt ccctttaaga
    16861 tctgcccagt caaagtcatc tccttgaaga tactcttcta ccgtttggtt aatcaattct
    16921 gaactttcac ggagaatatc gcgactcttc ttgagataaa caaatccgcg cgtatgaaca
    16981 cgagccctag ccacaatttt cttctcacga cggttgactg taatagccac gatgaaaatt
    17041 ccatcctctg acaagacctt acggtcacga agaacaacat ttccaacatc accaatggca
    17101 ttcccatcaa tcaagatatc tcctgctgaa accgatccag ctggaacaaa gtctccattc
    17161 tcgtaagcca tggtcgtccc ctttttagga atgaagatgc gttctggcaa catcccaact
    17221 gccatggcag ccttagcgtg agcatccaac tcacgatact ccccttggac agggaagagg
    17281 tacttaggtt gcaaaagatt gatcatcagc tgcaaatcac gcacatttcc gtgccctgat
    17341 acatgtaaac tttgggtaat caatttgaca accccacctg cctgataaat catattttcc
    17401 acacgcgcaa caaaggcttc tttagcaata gacggagccg tagcaatata gaccaggtcc
    17461 ccatccttga tttctacata acgatggcga ccaatcgaca tcttacgaag tccattgata
    17521 ggttcaccca tacgacctgt ctcaagaata atcaactcat ggtcttcaaa gcgagacata
    17581 tctttaggct taatcaagag aatttcgttg gctaaagaca acttcttaag acgaatcgct
    17641 gtgcggacga tattttcaat atcaaatcct gtcaagacga tacgtcgacc tgttttatcc
    17701 gcagcgtcaa aaatctgctg aatacgagaa agattactgg aaacagctgc aacgatgata
    17761 cgaccttccc agtcagcaat agtttgggta atttcatccc taacttcact ttcactagcc
    17821 acctgaatat tgctgtctgc attggccgaa tcactgagga gagccaggac gccgtcacga
    17881 ccaatctctg ccaaacgagc aaaatcagtt gcataagatt cactagccgt ttggtcaaat
    17941 ttgaagtcac ctgtataaac gatgcttcct tccgatgtct tcaagacaat tcccagactc
    18001 tctggaacgg agtaagtcgt agggaagaag gaaaccactg tcccaccaaa atcaatctcc
    18061 gtattctcat caatgacatg gaaatcatta aatttcttaa cggcatcatt tcctttgaca
    18121 aagagctttg ccaactcaat ggtcaactca gacccaaata caggaacttt agcctctgcc
    18181 aagagataag gtagagcacc aatggcatcc gcatgcccgt gggtcaagaa aaccccagca
    18241 atacggtcgc tattttcaaa aaggtaatcc atgtttggaa tcaccacatc gacccctaat
    18301 tgttcatttt caggatattt taaccctaca ttcaaaacaa aaatggactc tccaatttca
    18361 gcaatgtaca tattttttcc attctcacgc acaccaccaa gtgttgttaa actgatatta
    18421 ctcatttttc ctccaaaatc ttagtttatc ttgattatag ggcaactcta ccctcttatt
    18481 ctaaaagttc taatactttc agccgaatct tttcctacca ttataccatt tttttgatga
    18541 ttttcaaaat gaaggtctgt tttcaaaaaa gagttgattt cccaactctt tcctaacttc
    18601 tactcttttt ccatcaaaaa atcataaatt tcttgcaccg tacccagcgc catcatttct
    18661 tggtctttaa cggtttgttt gagattttgg taaatttgac tttctcctat ctcccgcttt
    18721 ggcgcctctt tattgactgt catcacacca tcttttatcg tcacaaagcc tagttcttca
    18781 aatacttgaa tcatcttgac cagcaagatt tgttgaatat taagatatgc agccaaatct
    18841 ttcagcttgt agcgaatatc aaactctggg aactggtaaa tagtcttgta caatttggca
    18901 aactgatctc tagtcccata acctgtcaga taataggcct tgtcaatatc atttttgaaa
    18961 tagacagcag agaaatgctg ttcctgaaaa atggtcttca gctgagtaat atcctctgga
    19021 atgtttttta cgacaacagc ttcactagcc gcaagatttg gcagttctcc aggaaaatcc
    19081 aagactggaa caccttctgg caagactgca ttttttccac gaatgttaaa aagttgaaca
    19141 ccttccactc gcgcatccat catcatcaac tggagggcag tttggccgtt ccattggttg
    19201 acagacaatt taaccgctaa ctctagattc ttggtttgag aaaactctgt cgcccatctg
    19261 ccttgaccaa aggctaccac ttcaaaactc acctcaccct tggaaatttt cagctttaga
    19321 tgggcattac ctgcccccat agtacgagca ctttcgacct gaaaattctt gatataaaaa
    19381 ataggtttct gattatccat tccaaaagga gctaaacgtt caaaactttt gaccgtttcc
    19441 aagctaagtg cctccaaatc caactcttca tctaggttta acttattctt gccaccagca
    19501 tctgcacctt tttcacgaac ataatcttcc aaaatctgag ataaatctga gagttgctca
    19561 acttccagcg tcatacccgc tgcacctgca tgacctccaa aggcgatgaa gaggtctcga
    19621 tagggatcca gagcttcaaa aatatcgacc gcttccacac tacgagcact gcccttggca
    19681 cgaccgtctt ctatattaag aacaatgact gtctgtccca attcttccaa taaacgacca
    19741 gccacgattc ctagaacccc aggattccag ccttccttgg ccaagacctg aaccttcttc
    19801 tcaggatcca ccatggtctt ggcttcttca tagatagact gaacgatttc cttgcgctct
    19861 tcgtttttct ggtgaatcat aagggcaatc tcatgcgctt cctcatcatc aaatccagtc
    19921 aacaaatcaa tggcaggatt gggatcatcc aagcgaccca aggcattcaa acgaggagca
    19981 atctggaaac caaccgtttc ttctgttact tcgttagcag caatcccagc catgtccagc
    20041 atttcttgca gaccaatgcg ctgggtatga cccaacattt ccagaccata ttgaactaag
    20101 atacgatttt catccgtcag actcaccata tctgcaatag ttccaatagc gaccaaatca
    20161 agcaattcca cttgcacttc ttctaacagg gcacaagcca acttgaaagc aactccacaa
    20221 ccagccaaat atttaaaagg ataatccgca tctggatgtt caggatggac aatagcataa
    20281 gcatctggca gggtttcagg tatggaatga tggtctgtca caatgacatc tactcccata
    20341 gactgagcca atgcaatagc ctcatgacca gcaaccccat tgtccaccgt cacaatcaag
    20401 gaaattcctt cttgctcgat aaagtattta taaacactag cattagggcc atagccatcg
    20461 gtaaaacgat ttggcaggta aactcggcac tccgcaccaa gttgttccaa actttccttc
    20521 acaatagaag ccgaagtcat gccatccgca tcatagtctc cataaacgag aatattttcc
    20581 ccttcttcaa tagcctgacg aatccgctcc actgccttgt ccatatcatg gagcagataa
    20641 gcatcatgta agtcctccaa ggaaggttct aaaaacttct tcagactttc ttggtcctga
    20701 atccctctct caaacaataa ccgagccacc tcaggaccca gtccagcctt cttggctatc
    20761 tttgtaaaat ccgcattttc tacctgcagg gcaaactgcc attcataagt aggtgttatc
    20821 aaaaagacat ccactctttc taagaattct attgcttcat tataacatga tttagacgtt
    20881 ttctctatcc agattgcttt tttataacat tttatagtat attgaaacta gaatagtaca
    20941 cctctacttc taaaatattg ttagaaatcg atttgattgt cccgatcgat ttttcctgtt
    21001 cttatatcac tatagtgaat tttttcagat atgatttgac aagaacattc ttttatcgta
    21061 gaatcatgtt ataaaatcta acacaaggga gatctctcat ggctttagta gaatttaaaa
    21121 acgtcgaaaa atattacgga gactaccacg cactccgcaa catcaatctc cgttttgaaa
    21181 aaaggacaag ttgttgtcct gcttggacct tctggctctg ggaagtccac tcttatccgt
    21241 acgatcaatg gtttagaggc tgttaacaaa ggaagtctcc tagtcaatgg gcaccaagtt
    21301 gctggtgcca gccagaaaga tttggtacct cttcgcaagg aagtcggcat ggtttttcaa
    21361 cattttaacc tttatccaca caaaacagtg ttagaaaacg taacacttgc acccattgaa
    21421 gttctaggaa ttgataaaaa agaagctgaa aaaaccgccc aaaaatatct ggaatttgta
    21481 aatatgtggg acaagaaaga ttcctatccc gccatgctat ctggtggaca aaaacagcgg
    21541 atcgccatcg ctcgtggtct tgctatgcat ccggaactcc tcctctttga tgaaccaaca
    21601 tctgctcttg atcctgagac tatcggagat gttctagcag ttatgcagaa actggcgcat
    21661 gatgggatga acatgatcat cgttacccac gaaatgggct ttgctcgaga ggttgcggac
    21721 cgcattatct ttatggccga cggagaagtt ttagtagata tgacagatgt cgatagcttt
    21781 tttgacaatc caagcgaacc tcgtgcccaa caattcctca gcaaaattat caaccacgaa
    21841 agtgacaaag tcaaataagg aggcgcctat gaaaaaaaat tctttttatc agcattattg
    21901 attagccttt tcggccttgt tgctgccaaa ccagtccagg ctaatactag tatcgcagac
    21961 attcaaaaaa gaggcgaact ggttgtcggc gtcaaacaag acgttcccaa ttttggttac
    22021 aaagatccca agaccggtac ttattctggt atcgaaaccg acttggccaa gatggtagct
    22081 gatgaactca aggtcaagat tcgctatgtg ccggttacag cacaaacccg cggccccctt
    22141 ctagacaatg aacaggtcga tatggatatc gcgaccttta ccatcacgga cgaacgcaaa
    22201 aaactctaca actttaccag tccctactac acagacgctt ctggattttt ggtcaataaa
    22261 tctgccaaaa tcaaaaagat tgaggaccta aacggcaaaa ccatcggagt cgcccaaggc
    22321 tccatcaccc aacgcctgat tactgaactg ggcaaaaaga aaggtctaac ctttaaattt
    22381 gtcgaacttg gttcctaccc agaattgatt acttcccttc acgctcatcg tattgatgcc
    22441 ttttccgttg accgctctat tctgtctggc tacactagta aacgcacaga actactagat
    22501 gatagtttca agccatctga ctacggtatt gttaccaaga aatcaaatac agagctcaac
    22561 gactatcttg ataacttggt tactaaatgg agcaaggatg gtagtttgca gaaactttat
    22621 gaccgttaca agctcaaacc atctagccat actgcagatt aaggaggaca ccccatgaca
    22681 gatttatcat cttggacagc ctattttcag gattttggac aatttttcaa tggtttcctc
    22741 tttactcttg ccctagcgat tggatccttt atcctcgcca tggtcttagg aatcttcttt
    22801 ggtgccatgt caaccagtaa acgtccaatt ttacgcattt tagctcgcat ctttgttgaa
    22861 ttttaccaaa atactcccct cttggtgcag tttgttattg ttttttatgg cctacctctt
    22921 atcagtgacc atatcatcat gattccaatt tattggacag ccgttctctg cgtgggactc
    22981 tatcacggcg cttatatcgc tgaggttatt cgttcaggga ttcagtctat tcctagtggt
    23041 cagatggagg ccgccttgtc gcaaggtttt acctatatca gtgccatgcg cttgattacc
    23101 ttgcctcagg ccttctgcat cattctacct ccattgacca accaaattgt taacctcatc
    23161 aagaacacct ctactgtagc tatcatctct ggagtagact tgatgtttgt gactaagtct
    23221 tggtcggctc tcaacggaaa ctatatccca gcctttttag gtgctgctct tctctacttt
    23281 gccctatgct tccctgttgc ccagtttggt cgcaagatgg agcaagccaa caaaaaagcc
    23341 tattcacttt aggaggttac tatggaatct attttagaag ttttaacccc agataaccta
    23401 gtctttatct ttaaaggatt tggcttgacc ctctatattt ctctgattgc catcatcctc
    23461 tctactatca tcggtacggt gctagctgtc atgagaaatg gcaaaaatcc tatcttacgc
    23521 attatttcca gtatttatat cgagtttgtg cgcaacgttc ccaaccttct ctggattttt
    23581 actatctttt tggtgttcaa aataaagtcc acaccagcag gtattacagc ctttactctc
    23641 tttacatcag cagccttggc tgagattatt cgaggcggtc tcaatgccgt agacaaggga
    23701 caatacgaag caggaatgtc acaaggcttc acctcagccc aaatcctcta ctacatcatt
    23761 ctcccacaag ccatccgcaa aatgctacca gccatcattt ctcagtttgt tcccgtgatt
    23821 aaggatacca gtctcctcta ctctgttatc gccctacaag aactctttgg agccagccaa
    23881 attctcatgg gccgttattt cgaaccagag caggtcttca gtctttacat cctgattgcc
    23941 ctcatctact tcagctttaa cctagcaatt tctagcctgt ctcatatgct agccaaacgt
    24001 tggcaacaag ctgcagaata atactcttcg aaaatctctt caaaccacgt ctgcttcacc
    24061 ttgccgtata tatgttactg acttcgtcag ttctatccac aacctcaaaa cggtgttttg
    24121 agcagcctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat aaacagccac
    24181 tctccagttc aattggagag tttttttgat gagagtagat atttatagac ctcaatttga
    24241 caccatacat tttctatgat aaaatgtaac aaatttattt acaacaaatc gaaatctaaa
    24301 tgaaatggaa ttttctatga aatcaatcaa aggactactc tttatcatag ctagttttat
    24361 cttgactctt ttgacttgga tgaacacttc tccccaattc atgattccag gactagcttt
    24421 aacaagccta tctctgactt ttatcctagc cactcgtctc ccactactag aaagctggtt
    24481 tcacggtttg gagaaggtct acaccgtcca caaattcaca gcctttctct ccatcatcct
    24541 actaatcttt cataacttta gtatgggcgg tttgtggggc tctcgcttag ctgctcagtt
    24601 tggcaatctt gccatctata tctttgccag catcatcctt gtcgcctatt taggcaaata
    24661 catccaatac gaagcttggc gatggattca ccgcctggtt tacctagcct atattttagg
    24721 actctttcac atctacatga taatgggcaa tcgtctcctt acatttaatc ttctaagttt
    24781 tcttgttggt agctatgccc ttttaggctt actagctggt ttttatatca tttttctata
    24841 tcaaaagatt tccttcccct atctagggaa aattacccat ctcaaacgct taaatcacga
    24901 tactagagaa attcaaatcc atcttagcag acctttcaac tatcaatcag gacaatttgc
    24961 ctttctaaag attttccaag aaggctttga aagtgctccg catccctttt ctatctcagg
    25021 aggtcatggt caaactcttt actttactgt taaaaattca ggcgaccata ccaagaatat
    25081 ctatgataat cttcaagccg gcagcaaagt aaccctagac agagcttacg gacacatgat
    25141 catagaagaa ggacgagaaa atcaggtttg gattgctgga ggtattggga tcaccccctt
    25201 catctcttac atccgtgaac atcctatttt agataaacgg gttcacttct actatagctt
    25261 ccgtggagaa gaaaatgcag tctaccttga tttactccgt gactatgctc agaaaaatcc
    25321 caattttgaa ctccatctaa tcgacagtac gaaagacggc tatcttaatt ttgaacaaaa
    25381 agaagtgccc gaacatgcaa ccgtctatat gtgtagtcct atttctatga tgaaggcact
    25441 tgccaaacag attaagaaac aaaatccaaa aacagagctt atttacgaag gattcaagtt
    25501 caaataagta atcatactct cctgggaaat cccaggagag ttttttatag ccaaatgaaa
    25561 taagacagga acgatggaat tttggcattc aaatcaattg ataagactat tttatagtgg
    25621 agtgaaatag gatacgaaca aattgattag gaaaatcaaa tgaatttata gaaatctttt
    25681 agcagttatg ttatcctatt ctagtttcaa aacgctatag aagcagcatt gtactagtct
    25741 agattcagtt tactatacta aaacgagtag cttgaaatca aaaaacccac cctcacaggc
    25801 aggttttatc tgtattattc agctagatta tgctttacct tctgaaccga atacgtcgat
    25861 acgttcttca accgatgctt ggatagcttt tacaccgtca gccaagaatt tacgtgggtc
    25921 gaagagtttt ttcttgtcgt attctgcttc gtttgcttcg tagtcacgag caaatttacg
    25981 agttgcgtta gcgaatgcga tttggcattc tgtgttaacg ttaactttgg caacaccaag
    26041 tttgatagct gcttggattt gctcatcagg aatacctgat ccaccgtgca atacgattgg
    26101 gaatcctgga agagcttctg tcaatttttg caagtggtca aggtcaagac cttcccagtt
    26161 tactgggtaa ggaccgtgga tgttaccgat accagctgcc aagaagtcga taccagtttc
    26221 aaccattgct ttagcgtctt cgattggagc caattcacct ttaccgatga ttccgtcttc
    26281 ttcaccaccg atagtaccaa cttcagcttc tactgagata cctttagcgt gtgctttttc
    26341 aacaacttct ttagccaatt taaggttttc ttcaactgga aggtgtgaac cgtcaaacat
    26401 gattgaagta taaccaactt cgatacactc aagtgcatct tcgtagtgac cgtggtcaag
    26461 gtggatagct actggtacag tgatacccat tgattcaaca aggttagcga tcaagttgcg
    26521 agcaactttg taaccaccca tgtatttagc agcacccatt gaagtttgga tcaaaactgg
    26581 agctttttta gcttctgctg cgcgcaagat agcttgagtc cactcaaggt tgtttgtgtt
    26641 aaatccacca actgcataac cgttgtcacg ggctgcttgg acaaattttt ctgctgaaac
    26701 gattgccatt ttatcaggcc tcctgtatat ttttatgggt catcccattt acattgttca
    26761 ttttatcact ttttgccaaa aaaatctagt ttttcccgca gtttcgattg attttcttct
    26821 aactccatct atgtaaaccc tttctctccc tagtcttggg cgacttttgg aaaagctata
    26881 aagaaggtta aacgattctc ctccatctcg aaacgataag ctaatttttc atgttctaat
    26941 agactcttaa ccacaaagag ccccatacca gaccccttga ccttgcgact ggcattgtca
    27001 gaaaaagact gggctagttt ttcttgttcc tctgagctac agctattttc gataaaaagt
    27061 tctccttctc tttctccaat tcgaactaag ccacctggaa cagagtgctt aatggcattg
    27121 ctgatgagat tagaaagaat caacttcata actgatgggt ttagataagc ctgctgatgg
    27181 gtcaaactat tgtctatctg gagctctctt tccttggcta gcaaggcata atctttgacc
    27241 agattttgcg tcatctggag gaggtcaatt gtttccctat catctcgcaa ttcctgcaca
    27301 gaagagaggg aaagtatctg cagaacatgg tgattgagtt catccacaat ccccaaggca
    27361 actcccagat actggtctct atccttataa cgaccgatat tctctctcat attttcgatt
    27421 aggattttca aactagccag cggtgttttc aattcatgag aagctcctcg taggaattcg
    27481 accttcatct tctccagctg gagaatggct tcattctttt catgcaagtc cgcaataaca
    27541 gtcaagagat gctggtagag gctattgatt tgttccttga gattacctat ctcatcctta
    27601 gaatccacgc gcaatcgcac ttgggaatcc aggtccatca tccgacgggt cacccgcttg
    27661 atttccaaaa tcggtgcaac aatagtccgg gcgtagatgt aggccaccaa gagggaaatc
    27721 agaaaggagg ccagcaaggt atagggaaga aactggagac tgatttgctc cgcttccttt
    27781 tgtaaatcca tggaagctag aaactggaga atcatagtac caccgtcttg cgttttcacc
    27841 tcgcgctcct caataaagag agaggttgtc tggcggtctg tgtccagagg aagactgtcc
    27901 ttgacttcta acttgtcctc ggtcatctca cctttgacgg tccccttgat atcactagtc
    27961 tgggaataca agtctaacac ttgctcgata ctctgcctat ctttcccttc tagggactgg
    28021 gcaatggctg ttgccttttg accaatggtt tcctgacgat gactcagata agtcgaagga
    28081 aaaagaaaat aaatagctaa atgaaggcag ataaccagaa cactaaatat cgagaaggta
    28141 tagataaata tctttgcaaa taaacctgtt cgtttcattt tcgctccaat ttataaccaa
    28201 cattgcgcac agtgaggata caatccaagt ctagcttttt ccgcaattcc ttgatataaa
    28261 catcaataac acggtcaaag ggaacctcat ctgtcgcttt ccagacggca tcgataatct
    28321 gagaccgagt caaggcccgg ccttcatttt tcactagata gtccagaatt tccaactctt
    28381 tggcattgat aggcacttct tgacctgcga ggcttgcact gtagctttca aagtccacct
    28441 tggtatcctt gtaagaaaag attcgtcctg tatcgtagta gcgcttgaaa atcgcgtcca
    28501 ccctcacttt taagagggag agagagaaag gtttttccag atagccatct gccaaagagg
    28561 caaaggcact catcttgtat tcctcatcct gaaaagctgt caacatcaag acaggaacct
    28621 gactggtttt acgaatctca gctaggactt ctaagccgtt aagcttgggc atctggatat
    28681 ccagtaaaac cagggccacc tcatagctag aaaattgctc cagagcttcc tgaccgtccg
    28741 ctgcctcaat agtttcatag ccacaatccg tcaaataatc actgaccccc tcacggatca
    28801 tctcttcatc ttctacaatt aaaattttca tactttaact gctctctatt ttttattttt
    28861 cttagaataa atacctactc tattttctat tatagtctct tgctggcctt ttgtctgtaa
    28921 gcaactgatc actagataaa acgttgtgaa attcctttct cataaattcc ataactttag
    28981 tatattatat ttaagcacta aagtacaaag aaagcaactg aaagcaatga ttttcaccac
    29041 tgctttcaga tttattttga attgttaaat agctattcct atccactatt cttgaataga
    29101 aacacaagat gcaatcttta ttccagactc attttttcaa atttattcac catccagcaa
    29161 gagctctttt ggttgttttc taaggagatt gcttgaagca agcgccataa cgagaaccac
    29221 tagaaccaag gcaaggacaa aaatgatgat aaagtctgat gtctgaatgg aaatgtctag
    29281 gctcgacaag gtcttgctaa agccatctac ttctgcaccg ccaccaaggt tagaggcttg
    29341 agccgcctta ctagcctgtt tggcaacacc tgaagtcaca ttggcaagga cagtgtttcc
    29401 aattgcacgg gcagtgtaat tagctaggaa gtaagcagaa actagagcag ggatagcaat
    29461 caagatagat tcggtgatga attgacccaa gatacttgcc tgcttgaggc cgatagagag
    29521 gagaattccc acttccttgc gacgggcgtt gatccaaagg ctgagcaaga gggcaaggag
    29581 gagaactgag aagctcaagc taccccagaa gaggaggttg gccatcttgt acataccaga
    29641 gatagattgc tcaagagctg ggtagttaga ggagctcttg acgagtgtgt agctcttcca
    29701 gttgatacca ctgatgccat tcaactcttt cataacatca tccaagttct tgtctgctgt
    29761 tacaaagaag gttgcgtccc cataaatggc tgtgtcttct gtgtatccat aaagttttgc
    29821 agcagtgtga atgtctgtaa tagctgtgtt ttcgtaaagt tcttgtgagt aggttactgc
    29881 tgacttatta tgaccatcaa agagtccctt gattgtcact tcaactgttt ccttggctcc
    29941 tttttcatta tctgcatcgt agatattaga gtccagttta accttgtccc ctactttcca
    30001 gccgtgtttg gctgccaagt ccttgtgcaa gaggatttta tccttgtcgt cgttggttaa
    30061 gtgctctcct tcgactagtt tataagaacc agagacaaac ttatcttctt tagaggagtc
    30121 attgacacct gtaatcatca agctacttcc aaaacgcttg gcacgatcag cagtgagatt
    30181 cttcttggtt tctggcgttt caatcaggtc atatccagtc aaatctccga tagcgttgat
    30241 acgtttgaca taagactcga tggccttgtt ttcggtgatt tttttgatgt cttcaccctt
    30301 gatattccca gcaccacgag gcgttccttg gttgacgcga cgattgattt gcatggagaa
    30361 gctattggtg atatttttaa aggtctcctg agaagccttg gcagtagctc ccttgattga
    30421 caagccgacc aaactcaagc tcgccatgag gagaataatc aggaagatga caatcgattt
    30481 gaaaaacttc cttgtaacat aggcaaatgc gttgtgtaac atagattccc tttctagatt
    30541 ttgttttaat cattctatca aaataagctc aaattattta ctagtattgc gcgtttcagt
    30601 cagtttctta tcctttaatt caagtgtaat atctgacgct tgtgccactt ctttactgtg
    30661 agttacgaca atcacacatt tacctgtttt ctgggcaagt gatttgagta gttcgacaat
    30721 atctccagca gttttaggat ccagatttcc tgttggctca tcagctagaa taactggagc
    30781 ttctgagacc aaactgcgag caatggcaac acgttgctgt tgaccacctg ataactggag
    30841 aacattccgc ttgatctggc tttcatccaa accaagctca agaagtgtat tcttgcttgc
    30901 ctttttgttg accaatcgga tattttccag cggagaaaga taatctatca agttataatt
    30961 ttgaaagacc agggaaatat ggtgcatgcg atggtaagaa tagcccttct tacgaatatc
    31021 ctctccttga aaaaggatag aaccttcaac aggactatct agaccagcaa gtagggacaa
    31081 gagtgtggat tttcctgctc ctgactcccc aataatactg taaaattttc cgggttcaaa
    31141 attataatcg atctgatata ggactgcttc agcagtattc ttataacggt aggtaacatc
    31201 ttgtaattgt aataaagtca tgatttctcc ttcttaacta atagatgata aaatttcttt
    31261 cggtgatttt ctaaataaga ataggaaaca aagggctaca gataagcaac taagcagaac
    31321 tagaaaaaca taggattctg caaaagataa gatactagtt gataaactgc ttgctttggc
    31381 tagtgtatct tgtaagcttg cctgatctcc acttgctagt agagtttgga gtaggtaagt
    31441 tgtgattccg tttcctgcaa caaatgctgg aagcaaagct ccaagagata ccaaaactac
    31501 ctctaaacag aattgtagga agatcgagct cttgcctttt ccaagtgcaa gtaaaatccc
    31561 cacttcatag acccgttctc tcaaccagag agacaaaacc agaattaagg ctccagctcc
    31621 tgctatcaac atcccataaa ggaagatggt caggaaggtt tggaaagttg caactgagtc
    31681 tttgatttgt tcaaaagcct tgttttcctt ttcgacttgg tagccttgat tttccaaggc
    31741 caagttttct acctgcttca tgagtccgtc catttcctta ggattttcta catagaagcg
    31801 tgctgcactg acttgagctt cactattgcc caaaagggtt tggctacttt catagtctgt
    31861 aaagacttga ttttcactga agtcagaaga caagcctgtg aatttctctt gttttttacc
    31921 agaaaagatg ccgataatct caaactctac tgtttgtcct tttccagatt cagactgacc
    31981 agcatccaag ccaatcttgt catgaagtga aagaccgttc ttcttagcca attcttcgtg
    32041 gataaggatt ttcttggaat cccctttttg aaggtgtcgc ccttctttta gattgaaagc
    32101 cgaactggta aaggttacat ccttggatga atcctcaaga gccgttaagc taaccaagtt
    32161 attgtctgca gctgataaat catcacgctc cacgctctgc tcgccagtca ctgcttcctt
    32221 gtcttttagt tttgcgaccg tctcaagttc aggagagaca ttttccagcc ccttaatctt
    32281 gcttacagat gctaggtctg acaacttgaa tgtctgacca ttctctatct tcttaataga
    32341 aaaagatgta ttgagtgatt tataaagatt gctttctact gttttgttgg acttcatcag
    32401 agtcaaacag gctgaaattc cggccaataa gaccaataaa atcagaaata aaataaaact
    32461 tctcagtcgc tttctgctga cataagccca agatctttgg attggattca tttgtcacct
    32521 ccatatttgt aagactatta taaaacccaa atatgaaatg tttatgaaat acgaaaaaaa
    32581 aatatcgagt aggggataat ctctagcccc tctcacacca ccgtacgtgc cgttcggcat
    32641 acggc
//
LOCUS       DAVKLX010000024        31571 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKLX010000024 DAVKLX010000000
VERSION     DAVKLX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31571
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.024"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(1..348)
                     /locus_tag="VW325_001137"
                     /pseudo
     CDS             complement(1..348)
                     /locus_tag="VW325_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            413..694
                     /locus_tag="VW325_001138"
     CDS             413..694
                     /locus_tag="VW325_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9734549.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWS
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            826..1770
                     /gene="manA"
                     /locus_tag="VW325_001139"
     CDS             826..1770
                     /gene="manA"
                     /locus_tag="VW325_001139"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9734550.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1792..3267)
                     /locus_tag="VW325_001140"
     CDS             complement(1792..3267)
                     /locus_tag="VW325_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEU9734551.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMNFLVPNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKGLE"
     gene            3433..3597
                     /locus_tag="VW325_001141"
     CDS             3433..3597
                     /locus_tag="VW325_001141"
                     /inference="COORDINATES: protein motif:HMM:NF024803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9734552.1"
                     /translation="MVPLKSENGYRTYSQEDLERLQVILYYKYLGFSLEKIAELLKEE
                     RTDLLLHLTR"
     gene            3598..4104
                     /locus_tag="VW325_001142"
     CDS             3598..4104
                     /locus_tag="VW325_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HEU9734553.1"
                     /translation="MDYLTRERQHLDTLISTLQKTIQEQKGERKMTIEEKFTGFSYQD
                     NQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAFNQVFQTLAQNLQVGLPATATENQ
                     EQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEFKENIDKFGSETAQYTSDAIAAYV
                     QTNAEKIG"
     gene            complement(4124..4579)
                     /locus_tag="VW325_001143"
     CDS             complement(4124..4579)
                     /locus_tag="VW325_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9734554.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4599..5774)
                     /locus_tag="VW325_001144"
     CDS             complement(4599..5774)
                     /locus_tag="VW325_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9734555.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5833..6678)
                     /locus_tag="VW325_001145"
     CDS             complement(5833..6678)
                     /locus_tag="VW325_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9734556.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6803..7360
                     /locus_tag="VW325_001146"
     CDS             6803..7360
                     /locus_tag="VW325_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9734557.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7379..7846
                     /locus_tag="VW325_001147"
     CDS             7379..7846
                     /locus_tag="VW325_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9734558.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7935..8564
                     /gene="upp"
                     /locus_tag="VW325_001148"
     CDS             7935..8564
                     /gene="upp"
                     /locus_tag="VW325_001148"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9734559.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8738..9328
                     /gene="clpP"
                     /locus_tag="VW325_001149"
     CDS             8738..9328
                     /gene="clpP"
                     /locus_tag="VW325_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9734560.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9407..9655
                     /locus_tag="VW325_001150"
     CDS             9407..9655
                     /locus_tag="VW325_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9734561.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9756..10916
                     /locus_tag="VW325_001151"
     CDS             9756..10916
                     /locus_tag="VW325_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9734562.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            11184..12053
                     /locus_tag="VW325_001152"
     CDS             11184..12053
                     /locus_tag="VW325_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9734563.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            12057..13013
                     /locus_tag="VW325_001153"
     CDS             12057..13013
                     /locus_tag="VW325_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9734564.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            13013..13777
                     /locus_tag="VW325_001154"
     CDS             13013..13777
                     /locus_tag="VW325_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734565.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13777..14487
                     /locus_tag="VW325_001155"
     CDS             13777..14487
                     /locus_tag="VW325_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734566.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14901..15557
                     /locus_tag="VW325_001156"
     CDS             14901..15557
                     /locus_tag="VW325_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9734567.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15665..16760
                     /gene="prfB"
                     /locus_tag="VW325_001157"
     CDS             join(15665..15736,15738..16760)
                     /gene="prfB"
                     /locus_tag="VW325_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9734568.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16778..17470
                     /gene="ftsE"
                     /locus_tag="VW325_001158"
     CDS             16778..17470
                     /gene="ftsE"
                     /locus_tag="VW325_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9734569.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17463..18389
                     /locus_tag="VW325_001159"
     CDS             17463..18389
                     /locus_tag="VW325_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734570.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
     gene            18675..20855
                     /locus_tag="VW325_001160"
     CDS             18675..20855
                     /locus_tag="VW325_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9734571.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVADLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20907..21722
                     /locus_tag="VW325_001161"
     CDS             20907..21722
                     /locus_tag="VW325_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9734572.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            21862..23205
                     /locus_tag="VW325_001162"
     CDS             21862..23205
                     /locus_tag="VW325_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9734573.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            23420..24610
                     /locus_tag="VW325_001163"
     CDS             23420..24610
                     /locus_tag="VW325_001163"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9734574.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            25163..26098
                     /locus_tag="VW325_001164"
     CDS             25163..26098
                     /locus_tag="VW325_001164"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9734575.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            26132..27169
                     /locus_tag="VW325_001165"
     CDS             26132..27169
                     /locus_tag="VW325_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9734576.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            27310..27915
                     /locus_tag="VW325_001166"
     CDS             27310..27915
                     /locus_tag="VW325_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9734577.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            28073..28603
                     /locus_tag="VW325_001167"
     CDS             28073..28603
                     /locus_tag="VW325_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9734578.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            28624..29526
                     /locus_tag="VW325_001168"
     CDS             28624..29526
                     /locus_tag="VW325_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HEU9734579.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGTDID
                     AACGQLRSNTMKRDRQKAVAAVNP"
     gene            29526..30008
                     /locus_tag="VW325_001169"
     CDS             29526..30008
                     /locus_tag="VW325_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9734580.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            30010..31551
                     /locus_tag="VW325_001170"
     CDS             30010..31551
                     /locus_tag="VW325_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9734581.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
BASE COUNT         9603 a         5900 c         6890 g         9178 t
ORIGIN      
        1 ctttctcttc ctcaactgtc atatatgcat ggttacgacc accacgtgtt tcttgaagga
       61 gagagtcgag tccgaactcc tcatattttt tacattttgc taaatcgttg tttgattaca
      121 gtctaaaagc tctataatct ctttataaga tttgcccatc agacgaaata taatagattg
      181 aaactagaat agtacacctc tacttctaaa acattgttag aaatcgattt gtcctgttct
      241 tgtttcattt tactatagaa cgatttgaag gcgtttataa tatttagctg tacgagagtc
      301 ttttaaaagt gttttgatgg tttggatttc ttctttagtt gatttcatat tactattgta
      361 taatgctttt tgattttagt ttagtattat cgtagaaaat ggactcggtg ctatggatac
      421 accggatgag aatggttatg tagcagatga ctatcggatt acttacttag aggcccacat
      481 caaggccatg cgagatgcca tttaccaaga tggggttgac ttgcttggtt atacgacttg
      541 gagctgtatc gatccggttt cagctggcac aggggaaatg aataagcgct atggctttat
      601 ctatgtagat cgagataatg taggaaatgg gactctcaaa cgtagcaaga agaagtcctt
      661 ctattggtac atgtcattta tagcaatggt gtaagcatcg agtagagcac atttgtttac
      721 tatttttata aaatcttctc cctgctttat aagatgtaaa aaagagttca aatggaactc
      781 tttttttgct ataatgaggg gagaaaaatc agacaggaga ttaagatgtc agaaccatta
      841 tttttacaat cagttatgca agaaaaaatc tggggtggta ccaagttacg tgatgagttt
      901 ggctacgaca tcccaagtga aaaagtcgga gagtactggg ctatctcagc tcatctaaat
      961 ggtgtttcta aagttgccaa tggtcgttac gagggaacag atcttgctac tttgtatgcg
     1021 gaacaccgtg aattatttgg caatcgtcca gaacctgtat ttccgctttt gaccaagatc
     1081 ctcgatgcca acgactggct cagtgtccaa gttcacccag acgatgctta tggactagag
     1141 catgaaggcg aacttggaaa aacagaatgc tggtacatca tcgctgcgga tgaaggttca
     1201 gagattatct acggccacaa tgccaagtca aaagaagaac tccgccagca aatcgaggat
     1261 aagaactggg atgcgctgtt gacaaaagtg ccagttaaag caggagattt cttctatgta
     1321 ccaagcggta ccatgcacgc tatcggggcg ggtatcttga ttcttgaaac ccagcagtct
     1381 agcgatacca cttaccgtgt ctatgacttt gaccgtaagg atgacaaggg caatttacgt
     1441 gaacttcacc ttgaaaaatc tatcgatgtc ttgaacattg gtgaacccgc aaatagccgt
     1501 ccagtgacaa tcaaagcaga tgatctacgt tccactctcc ttgtatccaa tgatttcttc
     1561 gcagtttaca aatgggaaat tactggaaaa gttaactttg aaaagacagc tgactacagc
     1621 ttgttgagtg tcttggctgg tcaaggtcaa ctaactgttg acgggaaaaa ctatccaatc
     1681 caaaaaggga gacactttat cctaccaagt gatgttgaag cttggactct ggaagggcaa
     1741 gatttagaat taattgttag tcatccataa aaaagaaagg gcctgagttc attactcaag
     1801 tcctttttga ttacataaat tgggcgatga agaccacgat gatgattgga atgacggaac
     1861 gaagaaacga agaaggaaga gccagacttg gaacagtcct tgtttccatg ctctttcatc
     1921 gagatggagt tcctccattg caagagcctt tttaaagata tagcctgtaa aaagtgaaag
     1981 gtagagagct ccaaatggca tgaggagatt gggaaccaag aagttcatag cgtcaaagaa
     2041 ggtcttacca aaaatgtgaa catccgccat gacaccgtaa gatagggctg aaggaatgcc
     2101 aaagacaaag gtcaaaattc ctaaaataac actccatttg gcacgtttgc tgttatcctg
     2161 gttggtgata ttgcccacat tgatctccag catcacgaca gaagaagtga cagtcgcaaa
     2221 gaggaagagg acgtagaaaa tggttccaaa aggcatcttg tcaaagagtt gaggcaagac
     2281 gataaagagc aagctcggtc ccccttcaga ctggatattg aagggggacc gagcttggaa
     2341 aatggctaga cctgccatga tggatatcga gatattcatg gctacgatgg agattcctga
     2401 ctggactaga ttggttttct tgtccaagta agaagcatag gtcaacatga ctgtaacccc
     2461 tagtgagagg gcaaagaaag attgtcccag agcatagagg agaccagcgc tagtcagttt
     2521 tgaaaagtct ggtttgagga agtaaagaac cccttccatg gcatttggca aactgagaga
     2581 gcgaccgatg ataacaacaa agatgataaa gagcaggggc atcatgactt tcgaagctct
     2641 ttcaatccct ttttgaaccc cacgtgatac aataaagata tttaataaga taaaggctgc
     2701 ttgagctcct agggcaatgg ctggatttga aatgattgaa gtaaataact gagcataatc
     2761 acccgttcca ccaagttgga acaatttccc aaactcaata cctagataga ctagaatcca
     2821 tcctccgata acactgtaaa aagataagag gataaagagg gcaaaggcgc caatccaacc
     2881 gataaagttg tacttgttat tcttgcccag ttttccaaag gttttgatag cggaaacgcc
     2941 agcactacgg ccaagggcaa actcagccag caggagaggg aaaccgatta aaatagtgga
     3001 aatgagaaag attagtaaaa agcctccacc gccattagca gcagtcatgt agggaaactt
     3061 ccaaacggaa ccaagcccga tgaccgagcc agcagatgct agaataaagc caagtttcga
     3121 cccccattgt gatttttctg acatagttaa actctgatct cctttttcaa aataagaaaa
     3181 agctacttga gttgtcaaat agccttctgt ttgattgata gacttgacta tcctattatg
     3241 ttttctaagg tctgtcaaga aaaccatttt caagttttca cacctttctc aaaaacgctt
     3301 gactctgacc taaggggagg ggttatacta tcattgtaag gaggaaatca tgtaccatat
     3361 aaaagaagct gcgcagcttt caggtgtctc tgtcaagacc ccgccctgca tcactatgac
     3421 aagataggac tcttggtccc cttaaagtcg gaaaacggct atcgaaccta tagtcaagag
     3481 gatttggaac gccttcaggt cattctttac tacaaatatc taggcttttc tttagagaaa
     3541 atagcagaac tgttaaagga agaaaggaca gatttattgc tccatttgac taggtagttg
     3601 gactatctaa ctcgcgaaag gcaacatctg gataccttga tttccacctt gcaaaaaact
     3661 attcaagaac aaaaaggaga aagaaaaatg accattgagg aaaaattcac gggatttagc
     3721 tatcaagaca atcaaaaata ccaccaagaa gcggtagaga aatatggtca agaagtcatg
     3781 ggacaagcgc tcgaacgcca aaaaggtcac gaagacgagg ctacggccgc ctttaaccaa
     3841 gtctttcaaa ctttggcaca aaatcttcaa gttggtttac ctgcaacagc aaccgaaaac
     3901 caggagcaag cagccaagct cttgcaagcc attcgcactt atggatttga ctgctctatt
     3961 gaggtattcg gtcatatcgg taaaggttac gtctacaacc cagagtttaa ggaaaacatt
     4021 gacaagtttg gttctgaaac agcccagtac acgtcagatg ccattgcggc ttacgttcag
     4081 acaaatgcag aaaaaatagg ctaggaattt cctagcctat tttttacttc aaatcataaa
     4141 gccagtcgtc accgtttttg tagtaaaaga attcactgag atcttcttct agaaacacac
     4201 gaagcatatc agacatatca tcggttgcaa gttttagatg agaaagattt tcaaagtcct
     4261 cccaccaaac tttcccttcg tctgaagact ggagttcacc agtaaagtgt tctgtcttgt
     4321 aaaaaaggac gacataacga taatccttgt cgtcatacca gtttttgata ccacagagtt
     4381 ggggtttgga aatgatcaga ccagtttctt ctttcacttc acgaatgaca gcatcgacaa
     4441 aggattcgcc acgttcaaca tgaccaccag gaaaagtaat gccagaccag tcgggactaa
     4501 ctcggtcttg gaccaggacc ttatctccgt ttttaatcat acacatgtta acaaattcga
     4561 ctgcctctct tctgttcatt cttcacaacc tttaatcttt aatcataatg cagacttccc
     4621 gccacccagc cggtacagag ggcagaagtg atgttaaagc cacccgtgtg ggcattgata
     4681 tccataactt cgcctgcaaa gtggaggcca ggtaccagct tactttcaag ggttttagga
     4741 ttgatttcct tgagactgac tccacccttg gtaacaaagg actttgcaag ggacattttt
     4801 ccagttacag gaattttaag ttctttaatg gactggacaa gttgttctcg ttccttttca
     4861 gtcagttgtt tgactttttc aggatatcct tgtacaaaaa attcggccaa gcgttctggt
     4921 aacaaggttt ttaaagcgtt tttcaaggat ttttcccgat tttcttctag aaatgtaacc
     4981 aagtccttct cagaaagttg aggcaaaaca tcgagtgaga gaacctcccc acctttgaca
     5041 aagctagaca tgcgtagggc agcaggacct gacaaaccaa agtgggtaaa gagtaaatca
     5101 tgagtgatga catgcttacc ataacttagg gtcacatcgt ccagagaaat cccttgtaag
     5161 gctttatgtg gaaaatctgt taataaagga ctttcagcag cctcaagatc ggtgatggta
     5221 tgcttaaaat ggcgagcaat ctcgtgacca aaaccagtcg aaccagtcga aggataagac
     5281 ttaccacctg ttgtgacaat gagtttctca caagtgaagg tttgatccgc tgacttaagg
     5341 acaaactggt catctacttt tttaacagaa acgatttcta tttgagtagc aacttgacca
     5401 cctagttcgg tgattttctt ttccaaagct tcgataatag tccgagactt gtcactggct
     5461 ggaaagacgc gtccgtggtc ttcgacctta agtttaacac cattttctgt aaaaaagttg
     5521 atgatgtcat gattatcgaa ctgggagaaa acactgtaaa gaaagcgtcc gtttccagga
     5581 attccagcta gcaggttgtc taagctacca ttgttggtca cattgcaacg tcccccacca
     5641 gtcccagcta atttttttcc aagtttccga tttttttcga tgaggagggt tttctgtcca
     5701 taaaagctac tggaaatcgt agccatcata ccagcaggtc ccccaccgat gacaatagta
     5761 tcaaaatgtt tcatagctct attgtaccac aaaaaaacaa gagatgatgg tcacctcttg
     5821 tcaagaatgc aattaatcaa tttcatagcc catcagcaaa ccgccctctt ctgcatagaa
     5881 actgcagaga ccagaggttg gtagaatttt aatatccgct tgtgggaagg tttcacggat
     5941 tcgctctgag agctgttgac aacatttttc gttattgcgt tgggccatga caatacggcc
     6001 accagcatat ccagctttta ctaactcatc ataggcagct tgaactgatt tctttgatcc
     6061 ccttgctttt tgtagcaatt cgagagttcc agtttcacta gcttttccga ccatacgaat
     6121 gttgagaagg ccaacgaccg taccgataag cttgctcaaa cggccgttct tcaccaagtt
     6181 atcgactttg gctaggacaa agagcaactt agttttttct tgataggcag tgatagcttc
     6241 aaccacttct tcaaaagaca agccctggtc aatcaagtca ttcaattttt ctacgagtag
     6301 gtcaacttca ccaccagcag ataaactatc aatcacatga atcttagtgt caggatggtc
     6361 ttctagataa atattctttg ctagttgagc actattgtga ctgccagaaa gggtacctgt
     6421 gatggttact aggaaaatgt ttttggcacc ttcaaatgct cgcaaatagt catctgggct
     6481 tggacaagcc gattttgaag cttctgcagt tgcatacatg gtttccatca tttggtcaat
     6541 atcgagactg gcgtcatcaa caaagacctg atcagctact tgaatggtta aggggacact
     6601 tacaaaggtt gtgttaatag ctggtgttgg cagttgacga taatcacaac cagagtcagc
     6661 aataattttc caagtcatag aaattctcca tctttgtcag ttatacattg acaaaggttc
     6721 tgtctttttt tacaattata tcatgaaaac ccttgaaaca aaagcatcat ccgtctgttg
     6781 tgacaagtag aaagaaaatg ttatgtctga acgtagaatc tctgaaaagt ctcttgaaaa
     6841 tctcagaaaa tcaaaccaag aatccaattt attaaccaga gaagccattg aaacagccct
     6901 cttgcaactc ttggaaaaaa aggagctgac caagattagt atttctgaat tggtcaaacg
     6961 tgcaggtgtt tcgcgtgcgg ccttttatcg caattatgac tccaaagagg aaattttaga
     7021 gagcgtcttt aaacgaactg tccacaatat tatggaacag ctgcatcatt acgatttaaa
     7081 gacagacctt tatttggttt gggttcacct tttccgggag gccagaaagg aagctagagt
     7141 gattcaattg gccttggatt accatctgga aaaaatcttt gtccaagcca tgcaggaatt
     7201 tctagaaaaa taccatggga aatcaaaagg tgtcagctct tatcttcatt ccttctggag
     7261 ctcagccatc gtctctgtcc ttctaaaatg gatcaaggat ggcatgaagg taccagctga
     7321 aaagattgca gatttaggtt taccattttt taaaaaatag agaaaaagga gaaaagagat
     7381 gactgaaaaa agactggcct gggatgagta ttttgcagcc caagccctac taattgcgaa
     7441 tcgttccact tgtaaacgtg ctaaagtggg cgcgattctg gtaaaagata ataaggttat
     7501 ttccactggt tacaatggtt cggtgtcagg gactgagcat tgtattgatc acgaatgttt
     7561 ggtcattgaa ggccactgtg ttcgcaccct tcacgctgag gtcaatgcta tccttcaagg
     7621 tgctgagcgt ggtgttccca aaggctttac agcctatgta acccattttc cttgtctcaa
     7681 ctgtacaaaa caattgcttc aggtcggctg caagcgcgtg gtttatatca accagtaccg
     7741 aatggacgac tacgcccaat acctttatca agaaaaaggg acagaattga cccatttacc
     7801 acttgagaca gtacagacag ctcttaaaga ggctgatcta atgtaaaaat tatcaaaaat
     7861 aaatggctta gaaagatttt taaaccgttt tttggtataa taagaagaat aaattgaaag
     7921 aaggaattcc aaaaatggga aaaattgaag ttattaatca cccactgatt caacacaaat
     7981 tgtcaatctt gcgtcgtaca gatacttcta caaaagcttt tcgtgagcta gtagatgaga
     8041 ttgcaatgtt gatggggtat gaagtacttc gtgatcttcc actagaagat gtggaaatcg
     8101 aaacaccaat tacaaaaaca gttcaaaaac aattggcagg taagaaattg gccatcgtcc
     8161 caatcttgcg tgcaggtatc gggatggttg atggtctctt gagcttggtt ccagctgcta
     8221 aagttggcca catcggtatg taccgtgatg aagaaacact tcaaccagtt gaatacttgg
     8281 tgaaattgcc tgaggacatt gaccaacgtc aaatttttat agtagaccca atgttggcaa
     8341 caggtggctc agcaatcttg gctgttgatt ctcttaaaaa acgtggcgca tcaaatatca
     8401 aatttgtctg ccttgtatct gctccagagg gtgtaaaagc ccttcaagaa gctcatccag
     8461 atgtagaaat ctttacagca gccttggatg aacgtttgaa cgaacacggt tatatcgttc
     8521 caggtcttgg agatgctgga gaccgcttgt tcggtacaaa ataagatcga aaagaagaat
     8581 gacttggaaa agtatttcca gtcacgaaag gaggttgggt ttttgtttct gtctaatgaa
     8641 agcagagcaa aaatttgacc ttttttgacc aagatattat aatagtctta tcttcagtca
     8701 tttgaccaac aaaattaaaa ctcaaaagga gaaatgaatg attcctgtag ttattgaaca
     8761 aacaagccgt ggagaacgtt cttacgatat ttactcacgt cttctcaaag accgcatcat
     8821 tatgctgaca ggtccggttg aagacaatat ggctaactct gttattgccc aattgctttt
     8881 cttggatgcc caagatagta caaaagatat ttacctttat gtcaatacac caggtggttc
     8941 tgtttcagct ggtttggcaa tcgtagatac catgaacttt atcaaggcag atgtccaaac
     9001 cattgttatg ggaatggctg catctatggg aacagtcatc gcatcaagtg gagcaaaagg
     9061 caaacgtttc atgcttccaa atgctgaata catgattcac caaccaatgg gcggtacagg
     9121 tggtggtacc caacaaactg atatggctat cgctgcagaa cacttgctca aaactcgtaa
     9181 taccttggaa aaaatcttgg ctgaaaattc aggtcagtca atggaaaaag tccatgcaga
     9241 tgcagaacgt gataactgga tgagcgccca ggaaacactt gaatatggct ttattgatga
     9301 aattatggcc aacaattcat tgaactaatg atgatagaag gcaaactcga ctgggtttgc
     9361 tttttttggt ataataggga gagatttctt agaaagagga tttatcatgt ttgaaaaagt
     9421 caatcgctct ggcttgatta tctatcttta ctataatcgt gatgccaaaa aactgcagga
     9481 ttatggagat attacctatc attccaagaa acatcgttac ttacaactct atgttccaac
     9541 tcaagaagtg gagcaattgg tcggacgctt gagcaaggaa aaatttatta aaaaagttag
     9601 ggtttgtcat atccaagagt tggaaacacc ctttgtgggc aatctttatc gataggaaaa
     9661 cgttatcatc gaaaaagttc aaggaaaaca ttgacaattt tctgataatt cggtatattc
     9721 ttaacagact atttaagaaa taaggagaca aaaagatgaa gaaaaaattt gccctatcgt
     9781 ttgtggcgct tgcaagtgta gcacttcttg cagcctgtgg agaagtgaag tctggagcag
     9841 tcaacactgc tggtaactca gtagaggaaa agacaattaa aatcgggttt aactttgaag
     9901 aatcaggttc tttagctgca tacggaacag ctgaacaaaa aggtgcccaa ttggctgttg
     9961 atgaaatcaa tgccgcaggt ggtatcgatg gaaaacaaat cgaagtagtc gataaagata
    10021 ataagtctga aacagctgag gctgcttcag ttacaactaa ccttgtaacc caatctaaag
    10081 tatcagcagt cgtaggacct gcaacatctg gtgcgactgc agctgcggta gcgaacgcta
    10141 caaaagcagg tgttcccttg atctcaccaa gtgcgactca agatggattg actaaaggtc
    10201 aagattacct ctttattgga actttccaag atagcttcca aggaaaaatt atctcaaact
    10261 atgtttctga aaaattaaat gctaagaaag ttgttcttta cactgacaat gccagtgact
    10321 atgctaaagg gattgccaaa tctttccgcg agtcatacaa gggtgaaatc gttgcagatg
    10381 aaactttcgt agcaggtgac acagacttcc aagcagccct tacaaaaatg aaagggaaag
    10441 actttgatgc tatcgttgtt cctggttact ataatgaggc tggtaagatt gtaaaccaag
    10501 cgcgtggtat gggaattgac aaaccaatcg ttggtggtga tggattcaac ggtgaggagt
    10561 ttgtacaaca agcaactgct gaaaaagcat caaacatcta ctttatctca ggcttctcaa
    10621 ctactgtaga agtttcagct aaagctaaag ccttccttga cgcttaccgt gctaagtaca
    10681 atgaagagcc ttcaacattt gcagccttgg cttatgattc agttcacctt gtagcaaacg
    10741 cagcaaaagg tgctaaaaat tcaggtgaaa tcaaggataa ccttgctaaa acaaaagatt
    10801 ttgaaggtgt aactggtcaa acaagcttcg atgcagacca caacacagtc aaaactgctt
    10861 acatgatgac catgaacaat ggtaaagttg aagcagcaga agttgtaaaa ccataataga
    10921 aaaatgttga aatagggaat gagcctttga ctcactccct gtttcgatat ttaatactct
    10981 tcgaaaatct cttcaaactg cgtcaacgtc gccttggatt atatatgtga ctgacttcgt
    11041 cagtcttatc tacaacctca aagcagtgct ttgagcaacc tgcggctagt ttcctagttt
    11101 gctctttgat tttcattgag tataagaacc tatcaaaaag tgagggaaac ccctcggaat
    11161 tataaataga aagagtgaat cttatgctcc aacaactcgt aaatggtttg attctaggta
    11221 gtgtttacgc gctgttagcc ctaggatata ccatggttta cggaattatc aagctcatca
    11281 acttcgccca tggtgatatt tatatgatgg gagcctttat cggttatttc ttgatcaatt
    11341 ctttccaaat gaatttcttt gtagcgctta ttgtagctat gctagcgaca gctattcttg
    11401 gtgtcgtgat tgagtttctt gcttaccgac ctttgcgcca ctctactcgt attgctgttt
    11461 tgattacggc tattggggtt tctttcctat tggagtatgg aatggtctat ctggttggtg
    11521 ccaatacccg tgccttccct caagcgattc aaacagttcg atatgatttg ggaccaatta
    11581 gcttaacaaa tgtgcagtta atgattttgg ctatttcctt gattttgatg attttgttac
    11641 aagtcattgt ccaaaagact aagatgggga aagccatgcg tgcagtatca gtagatagcg
    11701 acgcggcgca attgatgggg atcaatgtaa accgtacgat tagctttacc ttcgctttgg
    11761 gttctgctct tgcgggtgcg gctggtgttc tgattgctct ttattataac tctcttgagc
    11821 ctttgatggg ggttactcca ggtcttaaat ctttcgttgc cgcagtactt ggtggtatcg
    11881 gaattattcc tggtgcggct cttggtggtt ttgtgattgg tctattggaa acctttgcga
    11941 ctgcctttgg gatgtcagat ttccgtgatg ccattgttta tggaatcttg ttgttgatct
    12001 tgattgtccg cccagctggt atccttggta agaatgtgaa agagaaggtg taaacgatga
    12061 aggaaaattt aaaagttaat attctatggt tactcctttt gttagctggc tatagcttga
    12121 ttagtgtact ggtttcagtc ggagtactta atctattcta tgtacagatt ttacagcaaa
    12181 ttggaattaa tattattttg gctgttggtc tcaacttaat cgttggtttt tcaggacaat
    12241 tttcacttgg tcatgctggt ttcatggcga ttggtgccta tgcagcagct attattggtt
    12301 ctaaattacc aacctacggt gccttctttg gagctatgct tgtaggggct ttgctttcag
    12361 gagcagttgc cttacttgtc ggcattccaa ccttgcgctt gaagggggac tatcttgcgg
    12421 tagcaactct gggtgtttct gaaattatcc gtatctttat catcaatggt ggaagcctta
    12481 caaatggtgc ggcaggtatc ttagggattc ctaactttac aacttggcaa atggtttact
    12541 tctttgtcgt gattacaacc attgcaacct tgaacttctt gcgtagccca attggtcgtt
    12601 caaccctctc tgttcgtgaa gatgaaatcg ctgctgagtc agttggggtt aatacgacta
    12661 aaattaaaat catcgctttt gtctttggtg ccattactgc aagtattgct gggtcacttc
    12721 aggcaggatt tatcgggtct gttgtaccga aagattacac cttcatcaac tcaatcaacg
    12781 ttttgattat tgttgtattt ggtggactcg gttccattac aggtgcgatt gtttcggcta
    12841 ttgttctggg aattttgaat atgcttctcc aagatgttgc tagtgtgcgt atgattattt
    12901 acgctttggc cttggtattg gtaatgattt tcagaccagg tggactcctt ggaacatggg
    12961 aactgagcct atcacgtttc tttaaaaaat ctaagaagga ggaacaaaac taatggcatt
    13021 acttgaagta aaacagttaa ccaaacattt tggtggtcta acagctgttg gagatgtgac
    13081 tcttgaattg aacgaagggg aactggttgg attaatcggt ccaaacggag ctgggaaaac
    13141 cacccttttc aaccttttga ccggtgttta tgaaccaagc gagggaacag taaccctaga
    13201 tggtcacctt ttgaatggga aatcacctta taagattgcc tctttgggac ttggacgtac
    13261 tttccaaaat atccgtctct ttaaagattt aacagtttta gacaatgttt tgattgcttt
    13321 tggaaaccat cacaaacaac atgtttttac tagtttctta cgcttaccag ctttttacaa
    13381 gagtgaaaaa gaattaaagg ctaaagcttt ggaattgttg aaaatctttg atttagatgg
    13441 tgatgcagag actcttgcta aaaatctttc ctacggacaa caacgtcgtt tggaaattgt
    13501 tcgtgccctt gctacggaac ctaaaattct cttcttagat gaaccagcag caggtatgaa
    13561 cccacaggaa acagccgaat tgactgagtt aattcgtcgt atcaaagatg aatttaagat
    13621 tacaatcatg ttgattgaac acgatatgaa tctggtcatg gaagtaacag aacgtatcta
    13681 cgtacttgaa tatggccgtt taattgctca aggaactcca gacgaaatta agaccaataa
    13741 acgcgttatc gaagcttatc taggaggtga agcctaatgt ctatgttaaa agttgaaaat
    13801 ctttctgtgc attacggtat gatccaagca gtccgtgatg taagctttga agttaatgaa
    13861 ggagaagttg tttcccttat cggtgccaac ggtgcaggta agacaactat tcttcgcacc
    13921 ttgtcaggtt tggttcgacc aagttcagga aagattgaat ttttaggtca agaaatccaa
    13981 aaaatgccag ctcagaaaat tgtggcaggt ggtctttcac aagttccaga aggacgccac
    14041 gtctttcctg gcttgactgt tatggaaaat cttgaaatgg gagctttctt aaagaaaaat
    14101 cgtgaagaaa atcaagctaa cttgaagaag gttttctcac gctttcctcg tcttgaagaa
    14161 cgtaagaacc aagatgcagc tactctttca ggaggggaac aacaaatgct tgccatggga
    14221 cgcgctctta tgtcaacacc aaaacttctt cttttagatg aaccatcaat gggacttgcc
    14281 ccaatcttca tccaagagat ttttgatatc attcaagata ttcagaagca aggaacaacc
    14341 gtcctcttga ttgaacaaaa tgccaataaa gcacttgcaa tctctgaccg aggatatgta
    14401 ctggaaacag ggaaaatcgt cctatcagga acaggaaaag aactcgcctc atcagaagaa
    14461 gtcagaaaag catatctagg tggctaaaac aatccagtgg attgttttag tcggcagatg
    14521 aagattacga agtaatcatc attatagtct gggagactag tttaggttgc agataaagat
    14581 tgcgaagcaa tcatcaaatc cagtggattg ttttagtcgg cagatggaga ttacgaagta
    14641 atcatcaata tagtccgggg gaccttttta gtcggtagat tgagattgca aacaaatctg
    14701 catctacatt gaaagcttaa tttctaataa ttgaaaaaat cgaatgaaaa atttcttacc
    14761 ttcattcaca gagctcgatt tcagagctct ttttgctagc ttattcatac ttttctgaat
    14821 ttcgaaaaag aaatgtaagc gtttgataga tttacaaaaa gattgtataa tagggataag
    14881 aatagaaaag gagaagtctc atggcagtta aagattttat gacccgcaag gtagtttata
    14941 ttagtccaga tacaacagta tctcatgcag cagatttgat gagagagcaa ggtttgcacc
    15001 gtctgcctgt tatcgaaaat gatcaattag ttggtttggt gactgaggga accattgcac
    15061 aagcaagtcc atctaaagca acaagtcttt ctatctatga gatgaattat cttctgaata
    15121 agacaaaagt aaaagatgtc atgattcgcg atgttgtcac tgtctcaggc tatgctagtc
    15181 tagaagatgc aacttatctg atgttgaaaa ataagattag tattctccct gtcgtagata
    15241 accatcaagt atacggagtt attactgacc gtgacgtttt ccaagccttt cttgaaattg
    15301 caggttatgg cgaagaaggg attcgtgtac gctttgttac agaagatgaa gttggtgttc
    15361 ttggaaaaat tgtttctttg attgtagaag aaaatttgaa tatctcccat acagtcaata
    15421 ttccgcgtaa ggatggtaag gtgattatcg aagtgcaaat cgatggatca attgatttac
    15481 cagccttgaa agaaaaattt gaagcaaatg gtattcaagt ggaagaaatc gctcgcactt
    15541 cagcaaaagt cttgtaagaa gggaagccca aaggcttctt ttttcatgaa aaggggatta
    15601 gagaaaaaga tggaaagaaa tgataaaata tgctataatg aaataatgta aaaaaggagt
    15661 atttatggac atttcagtaa ttcgtcagaa aattgacgca aatcgtgaaa aattagcttc
    15721 tttcaggggg tctctttgac ctcgaagggc tagaggaaga gattgccatc ttggaaaaca
    15781 agatgacaga acctgatttt tggaacgata atattgcagc ccaaaaaacg tcgcaagaat
    15841 taaatgaatt aaaaaacact tacaatacct tccataagat ggaagagttg caggatgaag
    15901 tcgaaatttt attggatttt ttggctgaag acgagtcagt gcatgatgaa ctggtagcgc
    15961 agttagccga acttgataag ataatgacca gctacgagat gactctactc ttgtcagaac
    16021 cttatgacca caacaatgcc atcttggaaa tccatccagg ttctggtggt actgaggcgc
    16081 aggactgggg tgatatgttg cttcgtatgt atactcgtta tggtaatgct aaaggcttta
    16141 aagtggaagt gttggattac caagcaggtg atgaggctgg tattaagtcg gtaactttat
    16201 catttgaagg gcctaatgcc tatggtctcc tcaagtcaga aatgggtgtt caccgcttag
    16261 tgcgaatctc accatttgac tctgccaaac gtcgccatac ctctttcaca tctgtagaag
    16321 tgatgccaga attggatgat actattgaag tggaaatccg tgaagatgat atcaagatgg
    16381 ataccttccg ttcaggtggt gccggtggac aaaacgtcaa taaggtttca acaggtgtac
    16441 gtttaaccca cattccaact ggaattgttg tccaatcaac agtggatcgt acccagtatg
    16501 gaaatagaga tcgtgccatg aagatgttgc aggctaagct ctatcaaatg gagcaagaga
    16561 agaaggctgc agaggtagat tctctcaaag gtgagaaaaa ggagatcact tggggaagcc
    16621 aaatccgttc ttatgtcttc acgccttata ctatggtaaa agatcaccga actagctttg
    16681 aggttgctca ggtagataag gttatggatg gggacttaga tggttttatc gatgcttatc
    16741 tcaagtggcg aattagctaa gatagaaagg aactcacatg tcaattattg aaatgagaga
    16801 tgtcgttaaa aaatacgaca acggaacgac tgctctacgc ggtgtttcgg ttagcgttca
    16861 accgggggaa tttgcttaca tcgtaggacc ttcaggagca gggaagtcaa cttttattcg
    16921 ttctctgtat cgtgaagtaa aaatcgataa aggaagccta tcagttgctg gttttaatct
    16981 ggttaagatc aaaaagaaag atatcccgct tctacgtcgt agtgttgggg ttgtcttcca
    17041 ggattataaa ttgttaccaa agaaaactgt ctatgaaaat attgcttacg ctatggaagt
    17101 aatcggggaa aatcgccgta atatcaaaag acgagtgatg gaagttttgg acttggttgg
    17161 attgaagcat aaggttcgtt ctttcccaaa tgaactctca ggtggggagc aacagcggat
    17221 tgcgattgcg cgtgcaattg taaataatcc caaagtattg atagctgatg agccaacagg
    17281 aaatctggat ccggataatt catgggaaat tatgaatctt ttggaacgga ttaacctaca
    17341 aggaacaact attttgatgg cgactcataa tagccagatt gtaaatacct tgcgccaccg
    17401 tgtcattgcc attgaaaatg gccgtgtcgt tcgtgacgaa tcaaaaggag agtatggata
    17461 cgatgattag tagatttttt cgccatttat ttgaagcctt aaaaagtttg aaacgaaatg
    17521 gttggatgac agtagctgct gtcagttcag tcatgattac tttgaccttg gtggcaatat
    17581 ttgcatctgt tattttcaat acagcgaaac tagctacaga tattgaaaat aatgtccgtg
    17641 tagtagttta tatccgaaag gatgtggaag ataatagtca gacaattgaa aaagaaggtc
    17701 aaactgttac aaataatgac taccacaagg tatatgattc tttgaagaac atgtctacgg
    17761 ttaaaagtgt taccttttca agtaaagaag aacaatatga aaaattaacc gagataatgg
    17821 gagataactg gaaaatcttt gaaggagatg ccaatcctct ctatgatgcc tatattgtag
    17881 aggcaaacac tccaaatgat gtaaaaacta tagccgaaga tgctaaaaaa attgaaggtg
    17941 tctctgaggt tcaagatggc ggtgccaata cagaaagact cttcaagtta gcttcattta
    18001 tccgtgtttg gggactaggg attgctgctt tgttaatttt tatcgcagtt ttcttgattt
    18061 caaataccat tcgtattacc attatttccc gcagtcgcga aattcaaatc atgcgcttgg
    18121 tcggagctaa aaacagttat atccgtggac cgttcttgtt agaaggagcc tttatcggtt
    18181 tattgggagc tatcgcacca tctgttttgg tctttattgt ttatcaaatt gtttaccaat
    18241 ctgtcaacaa atcgttggta gggcaaaatc tatccatgat tagtccagat ttatttagtc
    18301 cgttgatgat tgccctacta tttgtgattg gggttttcat tggttcattg ggatcaggaa
    18361 tatccatgcg ccgattcttg aaggtttagg taaaatagct gcttttatga ggagattgta
    18421 aaatctcctt ttttgctaca agagtttttg aaaagagatg cgcagaagaa aagagcttcc
    18481 aaagaagtcc cccagagaag acttcttgat ggctaatttg aacttgaaat ttggcgcact
    18541 agaaattttt tagaaaaaag tgtaattttt ttataaaagc ctttacaaaa aaaattaaag
    18601 tggtataatt aaatcataga aagtgcaaac gttttcgtaa aacgtttgcc taaataaaaa
    18661 taaaggagat ttgaatgatg aaagatacat tcaaaaatgt cttgtctttc gaattttggc
    18721 aaaaattcgg taaggctttg atggtagtta tcgcggttat gccggctgct ggtttgatga
    18781 tttcaatcgg taagtctatc gtgatgatta acccaacctt tgcaccactt gtcatcacag
    18841 gtggaattct tgagcaaatc ggttgggggg ttatcggtaa ccttcacatt ttgtttgccc
    18901 tagccattgg aggaagctgg gctaaagaac gtgctggtgg tgctttcgcc gctggtcttg
    18961 ccttcatctt gattaaccgt atcactggta caatctttgg tgtatcaggc gatatgttga
    19021 aaaatccaga tgctatggta actactttct ttggtggttc aatcaaagtt gctgattact
    19081 ttatcagtgt tcttgaagct ccagccttga acatgggggt attcgtaggg attatctcag
    19141 gttttgtagg ggcaactgct tacaacaaat actacaactt ccgtaaactt cctgatgcac
    19201 tttcattctt caacgggaaa cgtttcgtac catttgtagt tattcttcgt tcagcaatcg
    19261 ctgcaattct acttgctgct ttctggccag tagttcaaac aggtatcaat aacttcggta
    19321 tctggattgc caactcacaa gaaactgctc caattcttgc accattcttg tatggtactt
    19381 tggaacgttt gctcttgcca tttggtcttc accacatgtt gactatccca atgaactaca
    19441 cagctcttgg tggtacttat gacattttaa ctggtgcagc taaaggtact caagtattcg
    19501 gtcaagaccc actatggctt gcatgggtag cagaccttgt aaaccttaaa ggtactgatg
    19561 ctagtcaata tcaacacttg ttagatacag tacatccagc tcgtttcaaa gttggacaaa
    19621 tgatcggttc attcggtatc ttgatgggtg tgattgttgc tatctaccgt aatgttgatg
    19681 ctgacaagaa acataaatac aaaggtatga tgattgcaac agctcttgca acattcttga
    19741 caggggttac tgaaccaatc gaatacatgt tcatgttcat cgcaacacct atgtatcttg
    19801 tttactcact tgttcaaggt gctgccttcg ctatggctga cgtcgtaaac ctacgtatgc
    19861 actcattcgg ttcaatcgag ttcttgactc gtacacctat tgcaatcagt gctggtattg
    19921 gtatggatat cgttaacttc gtttgggtaa ctgttctctt tgctgtaatc atgtacttta
    19981 tcgcaaactt catgattcaa aaattcaact acgcaactcc agggcgcaac ggaaactacg
    20041 aaactgctga aggttcagaa gaaaccagca gcgaagtgaa agttgcagca ggctctcaag
    20101 ctgtaaacat tatcaacctt cttggtggac gtgtaaacat cgttgatgtt gatgcatgta
    20161 tgactcgtct tcgtgtaact gttaaagatg cagataaagt aggaaatgca gagcaatgga
    20221 aagcagaagg agctatgggt cttgtgatga aaggacaagg ggttcaagct atctacggtc
    20281 caaaagctga cattttgaaa tctgatatcc aagatatcct tgattcaggt gaaatcattc
    20341 ctgaaactct tccaagccaa atgactgaag cacaacaaaa cactgttcac ttcaaagatc
    20401 ttactgagga agtttactca gtagcagacg gtcaagttgt tgctttggaa caagtaaagg
    20461 atccagtatt tgctcaaaaa atgatgggtg atggatttgc agtagaacct gcaaatggaa
    20521 acattgtatc tccagtttca ggtactgtgt caagcatctt cccaacaaaa catgcttttg
    20581 gtattgtgac ggaagcaggt cttgaagtat tggttcacat tggtttggac acagtaagtc
    20641 ttgaaggtaa accatttaca gttcatgttg ctgaaggaca aaaagttgca gcaggagatc
    20701 tccttgtcac agctgacttg gatgctatcc gtgcagcagg acgtgaaact tcaacagtag
    20761 ttgtcttcac aaatggtgat gcaattaaat cagttaaatt agaaaaaaca ggttctcttg
    20821 cagctaaaac agcagttgct aaagtagaat tgtaatatac ttgaggttgg aagctgtatt
    20881 ccaacctctt attttgggag aaaagaatga aatttttaac actcaatact cacagttgga
    20941 tggagaaaga agcagaggaa aaattccaga ttttgcttga agatattctt gaaaaggact
    21001 atgatttgat ttgtttccaa gaaatcaatc aggagatgac ctcgtcagag gtggaggtta
    21061 atgaccttta tcaagctttg ccagcagctg agcctattca ccaagaccat tatgttagac
    21121 tcttggttga aaagttgtta gagcaaggga aaaattacta ctggacctgg gcttataacc
    21181 atatcggcta tgaccgctac cacgaaggtg tggctatctt gtctaaaaca cctattgaag
    21241 ccagagaaat tttggtttca gatgtggatg atccaacaga ctatcatact cgccgtgttg
    21301 ccctagctga aactgtagtc gatggcaagg agctagcagt tgccagtgtt catctctctt
    21361 ggtgggataa aggtttccaa gaagaatggg cacgatttga ggctgtcttg aaaaaattga
    21421 acaagccact tttactagct ggagatttta acaacccagc aggtcaggaa ggttaccaag
    21481 ctattttagc tagtccatta ggcttacaag acgcatttga agttgctcaa gagaaaagtg
    21541 gtagctatac tgttccgcct gaaattgatg gctggaaagg gaacactgaa ccccttcgaa
    21601 tcgattatgt ctttactacc aaagagttag cggtggaaaa tttacatgtc gtatttgatg
    21661 gtaacaagag tccacaagtg agtgatcact atggcttgaa tgctatatta aactggaaat
    21721 aataactgaa aagaggttgg aactataaaa ttccagcctt ttcttactag agaagctact
    21781 ggaaatagcc taaataagtg agactactgt aatggaataa aatatggtat aattgataag
    21841 atagatagaa tcgaggatgt tatgtcattt acgaaatttc aatttaaaaa ctatattaga
    21901 gaagccttga aggagttaaa atttacaact ccaacagagg tgcaagacaa gttgattcct
    21961 attgttttgg caggtcgtga cctagtagga gaatcaaaaa caggttcagg taagactcat
    22021 actttcttgt taccgatttt ccagcaatta gatgaagcta gcgatagtgt acaagcagtg
    22081 attactgcac cgagtcgtga gttggctact caaatttacc aagtagcgcg tcagatttca
    22141 gctcactcag atgtcgaagt tcgtgtggtt aattatgtgg gtggtacgga taaggctcgc
    22201 cagattgaga aattggaaag caatcagcct catattgtta ttggaacacc aggccgtatc
    22261 tacgacttgg ttaaatctgg tgatttagct attcataaag ccaagacatt tgttgttgat
    22321 gaagcagata tgaccttgga tatgggattc ttggaaactg ttgataagat tgctggcagt
    22381 cttccaaaag acttgcaatt catggtcttc tcagcgacta tcccacaaaa actgcaacca
    22441 ttcttgaaaa aatacttatc aaatcctgtt atggagaaaa ttaagaccaa aacggttatt
    22501 tctgacacca ttgataattg gttgatttcg accaagggac atgataagaa tgctcaaatt
    22561 taccagttga ctcagttgat gcagccgtat ttggcaatga tttttgttaa cactaaaacg
    22621 cgtgctgatg aattgcattc atatctgact gctcaaggct tgaaggttgc aaaaatccat
    22681 ggagatattg cccctcgtga acgcaagcga atcatgaatc aggtgcaaaa tctggatttt
    22741 gagtatattg tcgcaacaga tttagcagcg cgtgggattg acattgaagg tgtcagccat
    22801 gtcatcaatg atgccattcc gcaagactta tctttttttg ttcatcgtgt tggtcgtact
    22861 ggacgaaatg gcctaccagg tacagctatt accctttatc agccaagtga tgactcggat
    22921 atccgtgagt tggagaaatt gggaatcaag tttagtccta agatggtcaa agacggggaa
    22981 tttcaagata cctatgaccg tgatcgtcgt gccaaccgtg agaaaaaaca agataaactt
    23041 gatatcgaaa tgattggttt ggttaaaaag aaaaagaaaa aagtcaaacc gggttataag
    23101 aagaaaattc aatgggcggt tgatgaaaag cgccgtaaaa ccaagcgtgc tgaaaatcgc
    23161 gctcgcggtc gtgcagagcg taaagctaaa cgccaaacat tttaatagaa attgttggag
    23221 tattgagctc caactttttg ctttatgaga acgaactatc taaaccgaaa cactacatta
    23281 aagactgcaa attgcgatta aaaatggtat aatgataaag ttatatagtc ccgataagat
    23341 ggtaggtatt tattacgaag agttttccta tcagtacttt gtaactctat aacaatattt
    23401 tttaaggggg gacattttta tgtcagaacg taaattattc acgtctgaat ctgtatctga
    23461 ggggcatccg gataagattg cagaccaaat ttcagatgcg attttggatg ctattttagc
    23521 aaaggatcca gaggcgcacg ttgctgctga aacagctgta tatactggtt ctgtccacgt
    23581 ttttggtgaa atttctacaa atgcctatgt ggatattaac cgtgtggttc gtgataccat
    23641 tgcagagatt ggttatacca atacagaata tggattttct gctgagacgg tgggagtaca
    23701 cccatctttg gtggaacaat ctcctgacat cgctcaaggt gttaacgaag ccttggaggt
    23761 tcgtggaaat gctgatcaag atccactgga cttgattgga gcaggtgacc aagggctcat
    23821 gtttggattt gcagtagatg aaacagaaga gctcatgcca ttgccaattg cactcagtca
    23881 taaattggtt cgtcgtctgg cagaacttcg taagtctgga gaaattagct atctccgtcc
    23941 agatgcaaaa tcacaagtta cagttgagta cgatgaaaat gaccgtccgg tacgtgtaga
    24001 tacagtcgtt atttctactc agcatgatcc agaggccact aatgaacaaa tccatcaaga
    24061 tgtgattaac aaggtcatca aagaagttat tccatcttct tatcttgatg ataagacaaa
    24121 attctttatc aatccgacag gtcgttttgt aatcggtggt cctcaagggg actcaggttt
    24181 gactggtcgt aagattattg tagatactta tggtggctac tctcgtcatg gtggtggtgc
    24241 cttctctggt aaagatgcga ctaaggtgga tcgttcagcc tcttatgcgg ctcgctatat
    24301 tgccaagaat atcgttgcag caggccttgc taagaaggca gaagtgcagt tggcctatgc
    24361 tatcggtgtt gcgcaacctg tttctgttcg tatcgatact ttcggtacag gaacagtagc
    24421 tgaaagtcaa cttgaaaaag cggctcgtca aatctttgac cttcgccctg cagggattat
    24481 ccaaatgctg gacctcaagc gtccaattta ccgtcaaaca tcggcttacg gtcacatggg
    24541 acgtacagat attgatcttc catgggaacg tttggataag gtagatgctt tgaaagaagc
    24601 agtaaaataa gattttaaga ggggaacgtc ctctcttttt tatagttttt aactatactg
    24661 ggatactgtt ctgaaaatcc attttgcgaa agtagagatt tacatgtata gtagattgaa
    24721 actagaatag tacacctcaa cttctaaaac attgttagca atcaatttga ctgtcctgat
    24781 cgatttctcc tgttcttgtt tcattttact atatttcttt aaaaatgata aaggttgaga
    24841 tttctcctcg taatagataa tcatggggat atttcaatcc aaagttttat tcgttatcac
    24901 ttgactattg caaggttttc tagagcaaca gagtcatgga atggactcat ggttgagatt
    24961 tctccttgtt gcttggactt cattcaaaag tctgttaccc aagccttgtt caaacttcca
    25021 atacactagc tgttttcata gcatgacttc tgtactagac tttcttttcc gaataaatag
    25081 atagaaccac agaatctagt aaacctagaa ttaaaattat ggtataatat tagcaataaa
    25141 agaaatctgg aggattagaa tcatggtatc aacgaaaaca caaattgctg gttttgagtt
    25201 tgacaattgc ttgatgaatg cagcaggtgt ggcttgtatg acgatagagg agttagaaga
    25261 ggtcaaaaac tcagcggcag gaacctttgt tactaagaca gcgaccttgg acttccgtca
    25321 ggggaatcct gagccacgct accaagatgt tccacttggt tccatcaact ctatgggctt
    25381 gccaaataat ggcttagact attatttgga ttatctttta gatttgcagg aaaaagagtc
    25441 gaaccgaact ttcttcttat ctctggtcgg catgtctcca gaggaaaccc atactatttt
    25501 gaaaaaagtt caagagagtg attttcgtgg tctgactgag ctaaatcttt cctgtccaaa
    25561 tgttccaggt aaacctcaga ttgcctatga ttttgagaca acagaccgga ttttggcaga
    25621 agtgtttgct tacttcacca aacctcttgg aattaaattg ccaccttatt ttgatattgt
    25681 tcactttgat caagcggcag ctattttcaa caaatatccg ctcaagtttg tcaactgcgt
    25741 taactctatc ggaaacggcc tctatataga agacgaatct gtcgttattc ggcctaagaa
    25801 tggttttggt ggaattggtg gagaatacat caaaccgact gctctagcca atgttcacgc
    25861 cttttatcaa cgtttaaatc ctcaaatcca aattatcgga acaggtggcg ttctgactgg
    25921 tcgagatgcc tttgaacaca tcctctgtgg agcaagtatg gtgcaggtgg gaacgaccct
    25981 tcacaaagaa ggcgtcagtg cttttgaccg cattaccaat gaactgaaag caatcatggt
    26041 ggaaaaaggc tacgagagct tagaagattt ccgtgggaaa ttgcgctata ttgactaaat
    26101 taaatcgaaa aatctgaaga aaggagagac gatgctagcc attgaagaaa gtcagaagtt
    26161 gactttatca aatttaccga gcctgagcct atttacaggg acagatcagg gtcagtttga
    26221 agtgatgaag agtcaagtgt tgaaacagat tgggtatgat tctgctgacc tcaactttgc
    26281 ctactttgat atgaaagaag tagtttacaa ggatgtggaa ctggagttgg tcagccttcc
    26341 tttctttgcg gatgagaaaa tcgtgatatt agattatttt atggatatca cgactgctaa
    26401 gaaacgcttt ttgacagatg atgagcttaa gtcatttgag gaataccttg acaatccttc
    26461 tccaacaacc aagttgataa tctttgcaga aggaaagctg gatagcaaaa gacggttagt
    26521 caaattactt aagcgtgatg ccaaggcctt cgatgcagta gaagtaaaag aacaagaatt
    26581 gcgccagtat ttccaaaagt ggagtcagaa acaaggtctg cagtttacca atcattcttt
    26641 tgaaaatctc ctcatcaagt cggggtttca atttagcgaa atccagaaaa atcttctctt
    26701 tttacagtcc tataaggcgg attctgttat cgaggaagag gatattgtta acgcaattcc
    26761 caagaccttg caggacaata tttttgattt aactcagttt attctgacta aaaagatgga
    26821 tcaggcgcgc gatttggtga gagacttgac cttgcaaggg gaagatgaaa tcaaactaat
    26881 tgcagtcatg ctgggacaat ttcggacttt tactcaggtg aagattttgg cggagtctgg
    26941 ccaaacagaa tcgcagattg caagtagttt aggtagttat ctgggacgta acccaaatcc
    27001 ttatcaaatc aagtttgcat taagagattc gagaggactt tctttgagct ttctgaagca
    27061 agctatttcc tatttgattg agacagacta tcagattaag acaggtcttt atgaaaaagg
    27121 tttccttttt gaaaaggcac tcttacagat tgctagtcag gtcaattgac atttgttgaa
    27181 actacaaccc gcgtcaggat tatcttgtcg tgggtttctt gctgatttgc ttatctataa
    27241 gcaaaaaact tgaaaaaagt gaatgtttgc gttatcattt tcatatagaa agaaaaagag
    27301 gtattacaga tggctattat cttaccagaa cttccatatg catacgacgc tttggaacca
    27361 tacatcgatg cggaaacaat gcatttgcac catgacaaac accatcaaac ttatgtcaac
    27421 aatgccaatg cagctttaga aaaacaccct gaaatcggtg aagaccttga agccttgctt
    27481 gctgatgtag aatctatccc agctgatatc cgtcaagcac ttatcaacaa tggtggcgga
    27541 cacttgaacc acgctctttt ctgggaattg atgactcccg agaaaacagc tccttcagca
    27601 gaactggcag cagcaatcga tgcaacattt ggttcatttg aagaattcca agcagccttc
    27661 actgcagcag caacaactcg ttttggttca ggttgggcat ggttggttgt caacaaagaa
    27721 gggaaacttg aagtgacttc aacagcaaac caagacacac caatctcaga aggtaaaaaa
    27781 ccaatcttgg gcttggacgt ttgggaacat gcttactacg tgaaataccg caacgtgcgt
    27841 cctgactaca tcaaagcttt cttttcagta atcaactgga ataaagtaga tgaattgtac
    27901 gcagctgcta aataatgata gttggaggga agaattgttc ttctcttttt taggttataa
    27961 gattctggtc tgacaaaatc gttagacttt tttcattttt tatgagaaac gtgctaactg
    28021 ctagaaatta tggtagaata aagtattaag taatcgtgga aaaaggatat ctatgcgaaa
    28081 agaaattgca cctgaattat acaactataa caagtttcct ggtccggagt tccatttaca
    28141 cggggacaag gtcgaaacgg aagggatagc tttttccttg gttgaaaata tcaaggatgc
    28201 ctttgatgtg acgactttta atcagcgttt ttcagaagtc ttaaccaagt ttgattatat
    28261 cgtgggggac tggagcaacg aacagcttcg cctacgaggt ttttacaagg atgaccgaac
    28321 agaagaaaaa cttgaaaaaa tcagtcgttt acaagactac cttttagagt attgtagtta
    28381 tggttgtgcc tattttgtct tagaaaatga agcccctaag cgagcatcat ttgacaagaa
    28441 aatgcgtaag acggaagaag aaacaccttc tagaaaagga aagaaaccga ctcaaactaa
    28501 acgaaagtcg aatgcagata agaaaaatag acgtcgtcag aaagaccagc attctcagaa
    28561 agaggacaag gaacaacgtc attttgtcat tcgtcagaag tgagtagaga ataaggagaa
    28621 gaaatgaaac cctcaattca tagtttggta catcaaacca tgcaggaatg ggtattggag
    28681 cagggagaaa agaaattccg tgcagatcaa atctgggaat ggctctaccg taaacgtgtg
    28741 cagtcatttg aagaaatgac caacctttcc aaggatttga ttgctaagct caatgaccag
    28801 tttgtggtca atcccttgaa acaacgtatt gtgcaagagt ctgctgatgg tacagtcaaa
    28861 tatctctttg aattgcctga cggtatgtta attgagactg tactcatgcg tcaacactat
    28921 ggtttatctg tctgtgtgac cactcaggtc ggctgtaata tcggttgtac cttctgtgcc
    28981 tctggtttga ttaagaagca gcgtgacctc aataacggtg aaatcgtagc gcaaatcatg
    29041 ctggttcaga aatactttgc tgatgaaggg gttcaggtca atcttgccgt gtctcttcac
    29101 gcacccaaca atgaacttcg ttcaagtatc atgaagatta accgtgcctt tccgattgaa
    29161 aaactctttg ctgctattga atactacatt gagacaacaa accgtcgtgt aacctttgaa
    29221 tatatcatgc ttaatgaagt caacgacggt gtagaacaag ccttggaatt ggctgaattg
    29281 ctcaagaaca tcaagaaatt gtcttatgta aacttgattc cttataaccc agttagtgag
    29341 catgaccaat atagccgtag tcccaaagag cgcgtgttgg ctttctatga tacgcttaag
    29401 aaaaaagggg tcaactgtgt ggttcgtcaa gagcatggta cagatattga cgcagcttgt
    29461 ggacaattgc gctctaacac aatgaaacgt gaccgccaga aggcagtcgc agcggtaaat
    29521 ccttaatgat gaagaagact tataatcata ttttggtctg gggagtcatt ttctatagca
    29581 tttgcattgt ctgtttttgc tttactcctc aagaacaatc taccgtggga gtgggaactc
    29641 caggtattca gcatcttgga cgcctggttt ttcttttgac tcctttcaat tctctctgga
    29701 aactgggcga agtgagtgac attggacaat tatgttggat ttttttacaa aatgtcctca
    29761 atgtcttctt gttttttcct ctgattttcc aactccttta tctatttcca aatttgcgga
    29821 aaacaaaaaa ggtccttctt tttagttttc ttgtgagtct tggaatcgag tgtacgcaat
    29881 taatcttgga ctttttcttt gatttcaatc gcgtctttga gattgatgat ttgtggacca
    29941 atactttggg tggctatctg gcttggctcc tttataaacg attacataaa aacaagataa
    30001 ggaattaaaa tgagtatttt agaagttaaa aatctgagtc acggttttgg tgaccgtgca
    30061 atttttgaag atgtgtcctt ccgtctcctc aagggagaac atatcggcct ggtcggtgcc
    30121 aatggtgaag gaaaatcaac ctttatgagt atcgtgactg gtaaaatgct gccagatgaa
    30181 ggaaaggttg agtggtccaa atatgtgacg gctggttact tggatcagca ctctgtcctt
    30241 gctgaaagac agtcggtgcg tgatgttctc cgtacggctt ttgatgagct tttcaaagct
    30301 gaagctcgta tcaatgacct ctatatgaaa atggctgaag acggcgcgga tgttgatgct
    30361 ctcatggaag aagtaggaga acttcaagac cgtctggaga gtcgtgattt ctataccttg
    30421 gatgctaaga ttgacgaagt agcgcgtgct cttggtgtta tggactttgg catggatacg
    30481 gatgtaactt ctttgtcagg tgggcaaaga accaaggtgc ttttggcaaa acttctcctt
    30541 gaaaagcctg atatcttgct gttggacgag ccgaccaact acttggatgc tgagcatatt
    30601 gattggctca agcgctatct ccaaaactat gagaatgcct ttgttctcat ttcgcacgat
    30661 attccattcc tcaatgacgt tattaatatt gtctatcatg tggaaaatca acagctgacg
    30721 cgttactctg gtgactacta ccagttccaa gaagtttatg ctatgaagaa atctcagcta
    30781 gaggcagcct acgaacgcca gcagaaagag attgcagacc tcaaggactt tgtggctcgt
    30841 aataaagccc gtgttgcaac tcgtaatatg gctatgtctc gtcaaaagaa attggataag
    30901 atggatatta tcgaactcca aagtgagaaa ccaaaaccat cctttgattt caaaccagct
    30961 cgtacaccag ggcgctttat cttccaagcc aagaacttgc aaattggtta cgaccgtcct
    31021 cttactaagc ctttaaatct taccttcgaa cgcaatcaaa aggttgcgat tattggtgct
    31081 aatggtattg gaaaaacaac tctcttgaag agtctcttgg gcattatctc gccaatcgct
    31141 ggggaagtgg agcgtggaga ttatttagaa cttggttatt ttgagcagga agtagaaggt
    31201 ggtaatcgcc aaactcctct tgaagctgtc tggaatgcct ttcctgccct taatcaagca
    31261 gaagtccgtg cagcccttgc ccgttgtggt ttgacaacca aacatattga aagccagatt
    31321 caagtattat cagggggaga gcaagccaag gttcgtttct gtctcttgat gaatcgtgaa
    31381 aacaacgttt tagtgctgga cgagccgacc aaccatttgg atgtggatgc aaaggatgag
    31441 ctcaaacgcg ctctcaaaga atacagggga tctatcctta tggtctgcca cgagccagac
    31501 ttttatgaag gttggataga ccaaatatgg gattttaata atttaactta agaatgagag
    31561 taaaagaaat a
//
LOCUS       DAVKLX010000025        29644 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKLX010000025 DAVKLX010000000
VERSION     DAVKLX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29644
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.025"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..375
                     /locus_tag="VW325_001171"
     CDS             <1..375
                     /locus_tag="VW325_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9734582.1"
                     /translation="PTFRMYLTNKEILNKLLSYSEDLKHHYQLYNKEPEKFFGLIEDN
                     LKQVHPIFQTVFKTFLKDKEKIINALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENF
                     KKRIFIALNIKKERTKFVLSRA"
     gene            complement(475..2097)
                     /gene="groL"
                     /locus_tag="VW325_001172"
     CDS             complement(475..2097)
                     /gene="groL"
                     /locus_tag="VW325_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9734583.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISNRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2113..2397)
                     /gene="groES"
                     /locus_tag="VW325_001173"
     CDS             complement(2113..2397)
                     /gene="groES"
                     /locus_tag="VW325_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9734584.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2570..2965)
                     /locus_tag="VW325_001174"
     CDS             complement(2570..2965)
                     /locus_tag="VW325_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9734585.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3043..3804)
                     /locus_tag="VW325_001175"
     CDS             complement(3043..3804)
                     /locus_tag="VW325_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9734586.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(3837..4466)
                     /locus_tag="VW325_001176"
     CDS             complement(3837..4466)
                     /locus_tag="VW325_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9734587.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKKENG
                     ETVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEI
                     VEMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPG
                     ELRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4482..4799)
                     /locus_tag="VW325_001177"
     CDS             complement(4482..4799)
                     /locus_tag="VW325_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9734588.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(4796..5095)
                     /locus_tag="VW325_001178"
     CDS             complement(4796..5095)
                     /locus_tag="VW325_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9734589.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5380..5571)
                     /locus_tag="VW325_001179"
     CDS             complement(5380..5571)
                     /locus_tag="VW325_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU9734590.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(5732..6406)
                     /locus_tag="VW325_001180"
     CDS             complement(5732..6406)
                     /locus_tag="VW325_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734591.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTAKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(6412..6858)
                     /locus_tag="VW325_001181"
     CDS             complement(6412..6858)
                     /locus_tag="VW325_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9734592.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(6972..7475)
                     /locus_tag="VW325_001182"
     CDS             complement(6972..7475)
                     /locus_tag="VW325_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9734593.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISVACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7472..7834)
                     /locus_tag="VW325_001183"
     CDS             complement(7472..7834)
                     /locus_tag="VW325_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEU9734594.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7900..9765)
                     /locus_tag="VW325_001184"
                     /pseudo
     CDS             complement(7900..9765)
                     /locus_tag="VW325_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(9775..10245)
                     /locus_tag="VW325_001185"
     CDS             complement(9775..10245)
                     /locus_tag="VW325_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9734595.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
     gene            complement(10369..11085)
                     /locus_tag="VW325_001186"
     CDS             complement(10369..11085)
                     /locus_tag="VW325_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9734596.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(11968..13383)
                     /gene="ply"
                     /locus_tag="VW325_001187"
     CDS             complement(11968..13383)
                     /gene="ply"
                     /locus_tag="VW325_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9734597.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13394..13726)
                     /locus_tag="VW325_001188"
     CDS             complement(13394..13726)
                     /locus_tag="VW325_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734598.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(13797..14405)
                     /locus_tag="VW325_001189"
     CDS             complement(13797..14405)
                     /locus_tag="VW325_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734599.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14418..14876)
                     /locus_tag="VW325_001190"
     CDS             complement(14418..14876)
                     /locus_tag="VW325_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9734600.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15001..15454
                     /locus_tag="VW325_001191"
                     /pseudo
     CDS             15001..15454
                     /locus_tag="VW325_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16131..16391)
                     /locus_tag="VW325_001192"
     CDS             complement(16131..16391)
                     /locus_tag="VW325_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734601.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(16450..17001)
                     /locus_tag="VW325_001193"
     CDS             complement(16450..17001)
                     /locus_tag="VW325_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734602.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(16991..17986)
                     /locus_tag="VW325_001194"
     CDS             complement(16991..17986)
                     /locus_tag="VW325_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734603.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLKGKMLEIWKYRPF
                     VSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            complement(18394..18819)
                     /locus_tag="VW325_001195"
     CDS             complement(18394..18819)
                     /locus_tag="VW325_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734604.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEVASISLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(18963..19331)
                     /locus_tag="VW325_001196"
     CDS             complement(18963..19331)
                     /locus_tag="VW325_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734605.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKVMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKK"
     gene            complement(19353..19559)
                     /locus_tag="VW325_001197"
     CDS             complement(19353..19559)
                     /locus_tag="VW325_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734606.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(19546..19707)
                     /locus_tag="VW325_001198"
     CDS             complement(19546..19707)
                     /locus_tag="VW325_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9734607.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(20065..21021)
                     /gene="lytA"
                     /locus_tag="VW325_001199"
     CDS             complement(20065..21021)
                     /gene="lytA"
                     /locus_tag="VW325_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9734608.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(21397..22767)
                     /locus_tag="VW325_001200"
     CDS             complement(21397..22767)
                     /locus_tag="VW325_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9734609.1"
                     /translation="MNKKRTVDLIHGPILLSLLSFAFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQSSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(23074..24240)
                     /gene="recA"
                     /locus_tag="VW325_001201"
     CDS             complement(23074..24240)
                     /gene="recA"
                     /locus_tag="VW325_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9734610.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            complement(24295..25551)
                     /locus_tag="VW325_001202"
     CDS             complement(24295..25551)
                     /locus_tag="VW325_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9734611.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FTLRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(25635..26651)
                     /locus_tag="VW325_001203"
     CDS             complement(25635..26651)
                     /locus_tag="VW325_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9734612.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(26659..27177)
                     /locus_tag="VW325_001204"
     CDS             complement(26659..27177)
                     /locus_tag="VW325_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9734613.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEATEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(27167..27610)
                     /gene="tsaE"
                     /locus_tag="VW325_001205"
     CDS             complement(27167..27610)
                     /gene="tsaE"
                     /locus_tag="VW325_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9734614.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVVILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(27696..28316)
                     /locus_tag="VW325_001206"
     CDS             complement(27696..28316)
                     /locus_tag="VW325_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734615.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(28699..29562)
                     /locus_tag="VW325_001207"
     CDS             complement(28699..29562)
                     /locus_tag="VW325_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9734616.1"
                     /translation="MSTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIQAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
BASE COUNT         8906 a         6611 c         5053 g         9074 t
ORIGIN      
        1 cctacttttc gtatgtactt aaccaataaa gagattttaa acaagctttt gagctattca
       61 gaagacttga aacaccacta tcagctctat aataaggaac cagagaaatt tttcggactt
      121 attgaggaca atcttaagca ggttcatcct atttttcaga ctgtctttaa aaccttcctc
      181 aaagataaag aaaagattat caacgccctt caactacact attctaatgc caaactggaa
      241 gcgaccaata atctcatcaa acttatcaag cgcaatgcct ttggttttcg aaactttgaa
      301 aacttcaaaa aacggatttt tatcgccctg aatatcaaaa aagaaaggac gaaatttgtc
      361 ctttctcgag cttagctttt tttcaaccca ctacagttga caaagagcct ataaaaagaa
      421 gggttagtca ttccctcctt ttgtgttttt tataagttgt tttctataga aagcttacat
      481 catcccgccc atcatacttg gatccattgc tggagctggg gctactggtt ctggtttatt
      541 ggctacgact gcttctgttg tcaaaatcaa gctggctaca gatgctgcat tttgtagggc
      601 tgaacgactc actttaactg gatcaatgat accttgatca atcatgttaa cccactcgcc
      661 agttgctgcg ttgaatccta taccaagctc agcatttttc aaacgatcga taacgataga
      721 tccttcaaat cctgcattgt gagcaatttg acgaacgggt tcttccaaag cacggagaac
      781 aatattacgt cctgttgctt catctcctgt caattccaag gtagcaacag ctggaatcac
      841 attggcaaga gctgttccac cacctgcaac aataccttct tcaacagctg cacgagtagc
      901 gttgagggca tcttcaatgc ggagtttcat ttctttcaac tcagtttcag ttgcggctcc
      961 gaccttaata accgctacac cacctgacaa tttggccaag cgttcttgca atttttcacg
     1021 gtcaaattca gaagttgtag tttcgatttg agacttgata accgcaacac ggttagaaat
     1081 cgcttcagga tttcctgcac cttctacaat aaccgtgcta tctttgtcca cggtcactct
     1141 cgctgcttga ccaagagctt caattgtcgc atctttcaac tcaagaccaa ggtcttctgt
     1201 gataactgtt ccgcctgtta agatggcgat atcttcaagc atggctttgc gacggtcacc
     1261 aaaaccaggt gccttgactg ctactacgtt gaaggttcca cgaatcttgt tcaaaacaag
     1321 agttggaaga gcctcgccat ccacatcatc cgcaataatc aagagtggac gattgctttg
     1381 gagaatgctt tccaaaagtg gcaagatttc ttggatattg gaaattttct tgtctgtaat
     1441 caaaatgtac ggattttcaa ggtcagccac cattttttcg ctatcagtca ccatgtactg
     1501 tgaaaggtaa ccacggtcaa actgcattcc ttctacgact tcaagctctg tttccatacc
     1561 acgtgactct tcgatggtga tgacaccgtc tttgccaact ttttccattg cttcagagat
     1621 gtactcacca actttttcag aacgagaaga tacggctgca acttgagcga tagcttcttt
     1681 attggcaaca gggatggcgt tgtttttcaa agcttcaact gctgcggcaa ctgctgtttc
     1741 aatcccacga cgaataccga ttggatttgc acctgctgtg acgtttttga ttccttcacg
     1801 gacgatagct tgggtcaaga ctgttgcagt cgtagtcccg tcacctgcga tatcattggt
     1861 tttagaagct acttctgata ctaacttagc acccatattt tcaaaatggt cttccaattc
     1921 gatttctttg gcaatggtca caccgtcatt ggtaatcaag ggtgaaccga atgacttttc
     1981 aagaacgaca ttgcgacctt ttggtcccaa ggttacttta acagtgtctg caaggatatc
     2041 gacaccacga accatggctg aacgggcatc tgatgaaaat ttaatttctt ttgacatact
     2101 tactttctcc ttctattcct caatgattgc caaaatgtta gcttcgccta cgatgatgta
     2161 cttttcatcg ccatctttga catcaagacc tgcgtgggct tcaactaaga cacgatctcc
     2221 agttttaaca cttggagcaa ccaagtcacc gttcaaggta cgaacacctt gtccagtagc
     2281 cacaacttgg gctgttttgg ttttttcttg ggctgaacct gcgagaacaa agcctccaac
     2341 agtttgttct ttttcttcta cttttaagac cacacggtcc cctaatggtt tcaacatctg
     2401 atttcctcca taatctgatt tcctccataa tgagatagat attattagca ctctttatac
     2461 ctgagtgcta attcatagtt ctattgtatc acttagtcag aaataatcaa gaaaaaagtc
     2521 tgactttctc aagataaaaa gcctgagacc aactcagact ttttaattct taaaatggca
     2581 attcttcctc ttccaagacc aaatctgcca aatcttggcc tgcattattt tcacgcatag
     2641 cacgttgggc acgactttcc aagagttgga atcctgtcac aaggacttcg gttacataat
     2701 tcatctgacc atttttctca aagcgacggg tacgcaattc tccatcaacg gaaatgagac
     2761 tacctttggt tgcgtagctt gccaaagttt ctgctagtct gccccataga accatattga
     2821 caaaatcagc ttcacgttca ccgttttggt ctttgtaacg acggttgaca gcgatagttg
     2881 ctcgcgctac tgacttgtca ttgttggttt tgtgcaattc tggtgtagac gttaaacgtc
     2941 caatcaagat aactttatta tacatatttt cttcctccta cttatctatt cgtaggaaat
     3001 caaaaaaagt tacagaaatt tgtaactttt cgagaaaatt ttttattttt tatgaaccat
     3061 gaaacctgtc gcctgttgat tggccataat ggtcatatct gtaatctgaa cacgacgagg
     3121 ttgactagtc acatagacta ctgtatctgc aatatcctga gcttgcaaag cttctattcc
     3181 ttggtaaacg gacgcagctc gttctttatc accatgaaaa cgcactgtag aaaaatctgt
     3241 ttcgacaatt ccaggctgaa tggtcgtcac cttgatatcc gttgcgatgg tatcaattcg
     3301 cagtccatcc gaaaaggtct taaccgctgc cttggtagct gagtaaacag cggcaccagc
     3361 ataggcgtag attcctgcgg ttgaccccat atttataata tgaccttgat tgacttttat
     3421 cattgctggc aagaaacagc gagtaaccgc catcagacct ttaacattgg tatccaacat
     3481 ggtcaacata tccaactcct catagtcttg atagggagcc aagtcaagag ccagtccggc
     3541 gttgttgacc aagatatcaa tcctccctat cgtttctaga atatcagagc aaacagtctt
     3601 caccatgacc atatccgtca catctagtgg aaaagtccag acggtttgat ttggaaagct
     3661 tactgcaaac tccgacttga ggatttctaa tctgtctatc cgtcgtcctg ttagaacgac
     3721 atcctcaccc tgctccaaat aagcacgcgc aatcgcttca ccgattcctg aggttgctcc
     3781 cgtaatcact acattttttg ccatcttatt tccttctatc agatgctgac aagttcctag
     3841 gcagtccagt gtttcgctgg gtcgaacggt gttccgacaa cttggtcttc tgataattca
     3901 agcaccccac gtttttgcgg agcatttggc aaatgcaatt cacgaggact acacatcatc
     3961 ccaaaactct tttcaccacg aagttcgcct gggaaaatga aattcccttt tggcatcatg
     4021 gcaccaggaa gagccacaat ggttttcaac ccaacacgcg cattaggtgc tcctgcaacg
     4081 atttgcacta tcttgtcact tgcgactgca acttggcaga tgttgaggtg gtcactgtct
     4141 ggatgagcta ccatctcgac aatttcacca acaacaaact taggttcctt atcattaaca
     4201 atttcttctg taaaaccttc cgcctgtaat tcttggttca aacgagcgac ttgctcatct
     4261 gataaaaaga cttgaccgcg ctctgcaatt tcaaacaagc tcgaaacctc aaaaatgttc
     4321 caagctactg tttccccatt ttcttttttg aggaaaacac gggctacctt gcctttgcgt
     4381 tccacattca gtttggcatc tccgctattt ttcacgatga ccataaggac atcaccgaca
     4441 tgttctttgt tatacgtaaa aatcattgtt tcctttttct cctatttcaa tcctgctaaa
     4501 aaatcgttaa tttgctcctt acttttacgg tcgcgattga caaaacgacc gatttccttg
     4561 tccttttcta gaacaacaag gctaggaatt ccgtaaacat cccagagttt ggccaaatcc
     4621 atatactgat ctcggtccat tcgaataaag gtgaactctg gattggtctc ctcaatctct
     4681 ggtaaggcag gataaatata acgacaatcg ccacaccagt ctgccacaaa aaggaagacc
     4741 ttcttgcccg ctttttccac taaagatgct aattcttcta aacttgctgg ctgtatcata
     4801 agacttcctc ctcatagact aggtcttcat tttcatagac aaaggtataa tgacggccat
     4861 cctcaaaaat gacgccacca accaagctct ccagactgct ttcgtaaact tgaacataga
     4921 gggtcgcaat ttcccccatg tcggaaaaat ggtctcgcac aatctctgtc aactcttcct
     4981 gagtcttcat gagcttacgg tcatctgcaa cttttttcgt ggcaagagca aggcttccga
     5041 tacctagcag agccaagcct gccatccaca tttttttagc tttcatacca ttcattttaa
     5101 cacaaaaaag gcttcaggac aaatgaggaa gcagcagaaa agcaagtaaa aagcctcttc
     5161 ctttaaggaa aaggacttct tatactaaga ccaaactaga aagctagccg caggctgctc
     5221 aaaacacggt tttgaggttg tagataaggc gacgctgacg tggtttgaag agattttcaa
     5281 agagtattat tcttattgcc aggcacctaa gttgccaacg tagtaaccat caggtgtgta
     5341 ggtattgcga aacatcttac ctgatgaagc cagataatac tacttgccat tgtctttgac
     5401 ccaatcattc gcaatcatgg aaccagaaga acttacataa taccattctc ccttgtcata
     5461 aacccaagta ctgactttca tggttcctga gcaattaaag gcaaaaaaac tgtccaatat
     5521 cattcgtttt ttaaaagcat ttgacactac atcatctacg ctgtagtaca tagatacatc
     5581 aaacgtaaaa gccaaaatat cgaaaaatat acaactttta aaggcagcat ttcatttttt
     5641 aaagtctaac aaccatagaa ctgaaatcag agcaaggcat caatttccta ctcttagtac
     5701 gtctattttg atggttgaag tgaagatttt tttatttaac gtagacaatt tccaacttcc
     5761 caaaagccac atctccacgg atagtcaagg ttttgctggt tggggctaca ggagcgtcag
     5821 ccttagctgc accaaaggag gtttctactt tcaaatctac acgccagtgt tgtggaacat
     5881 agacggttgc atttccgaag gccacttcaa tatttaaagt tgcaaaatca cctagcatct
     5941 ctgcattatc atagtagatt ttagcatccc caaaagcgac ttccacttgg tcatctacga
     6001 gatcttgatc ttgcttatag aaggtcccgc taccaaaagc gacttcctta gccgtgacga
     6061 ctgttttttt cccattgtac caccattttt tttcattcca gaacttactt gaatgcgtca
     6121 gataaccaac accaagtacc accaagatgc tagcccaaat aagagaatga ttggtaactg
     6181 gtaacaagtc ataagcgtaa tttgcaatga tgagcgccag taaacctgta aaaactgccg
     6241 aagtgagatg acgtctaagg atggactcaa tggacttata agcaaaaaaa acaataccta
     6301 gtaaaggcca tattttacca tccaaagaag gaattccaaa attcccttgc agcaagatcc
     6361 aagctgctaa aaccaataaa acaataccaa atgctttctt tttcatgttt tttacctcat
     6421 gtttcttaga ttttctttta ggagggattg gtaatgccgt gagacatgaa cctccttgtg
     6481 cgtctgataa aaggaaatgg tgcccgttcc tgaaaaggac ttgtccactg agtaaatctg
     6541 acggatgttt gcgatcgttg acttggaaac tcgattaaaa tagcgaggca agatagactc
     6601 caactcatag agcttgaggc gaacctcata agcttccttc tggttatgag cgtagatctt
     6661 gctcccttct gtttcaaaga agagaatttc tgacaggtct agataatatt cacctgtccc
     6721 cttgtaaaaa gtcaacctcg gagcctttga ctcttgcaag agccgttgca agtctgcaat
     6781 ttcatctgtc aaagtggggg tcttgatgac aatttcagtc tcctctaaat tgccgtcaat
     6841 ctcaattcgt aacttcatca catctccttt ctgactctac tatatcaaag ctaagttagg
     6901 aatacaaggg caaaaaagca agtggtcaat ttgaagcagt aagtggttgt aggactagct
     6961 taacttacat gtcagaatag gaagaaaagg aatccccaca ccaaaccaca agcatagcca
     7021 accaagacat ccttgggata atgaacaccc cctaaaaccc tcaccaaccc cagcaaagct
     7081 gaaaagatca agcatactaa gccaataggt agactagcat gcagacaggc cacggagatg
     7141 atagttgccg aaaagacatg gcgactgggc atcgaccgtc ccgaactatc cttatcaaga
     7201 aggggacaga tatcccaagt ttcataaggt ctcgggtgat tgattttctt acgaaacagg
     7261 gacaaggcca caaaacctga tgcaggaata agcacataaa ccccaacttg ctgaccaagt
     7321 cctagttgca ggtaagtggt gactagcaag atcagataga ccaggggcat ggcgactgtc
     7381 atcaaacgat tgaaactgcg taataggaat aaaagaattg gacgactagt tagtctggag
     7441 ctgatatttc gataccattc ttgataattt ttcatttatg cttctcatac ttttctagaa
     7501 cttgttgaga tgttttcccc ttggcaagcc aatccactag cttgtccaag cgacgtattt
     7561 cctgcatcag aggatcatca atttcctcaa tacggacacc gcaaatcttc cctgtaattg
     7621 caattcgttc agggacataa tatggtgctt gtctaaaaaa atctccgtaa gtaacatccc
     7681 tatccagaca agcaagaaca tcactcactt cataccctgt caaccaactg gtcacttgat
     7741 gaactgactc tgcctttcca ccctttcgct caacttttgc aatcaaggct aagtagacag
     7801 cagcaaattt catattgtat aatttctgac tcatacttct ctctttctat gtgtactagt
     7861 ataaataaga aaaagaaggc cgtcaagcct tcttttgatt tattcttctg cttcatcttc
     7921 tgtaaattga ctattgtaca agtcagcgta gaagccacct tgcgccatca gttcctcata
     7981 gttgccttgc tcgatgatat ttccatcttt catgaccaag atcaagtctg catttcggat
     8041 ggttgacaag cggtgggcaa tgacaaagaa tgtgcgtcct tccatcaaac ggtccatggc
     8101 tttttggatc aattcctctg tccgtgtgtc aacagaagaa gtcgcctcat ccaaaatcaa
     8161 aagcggtgca tccttaagaa gggcacgagc aatagtcaat agttgttttt gtcctacaga
     8221 caaggtcacg gtgtcatcca agatggtatc atagccatct ggcaaggtca taataaagtg
     8281 gtgaattccc acagccttac tagcttccat cattcgttca tcactaatcc ctatttgatt
     8341 atagatgaga ttgtctcgaa tagttccttc aaagagccag gtatcctgca agaccattga
     8401 aaaggcatca tgcacttctg aacgcgtcat agccttggta tccacaccat caatgtgaat
     8461 acttccctta tcaatctcat agaatttcat caaaagattg acaatggttg tcttaccagt
     8521 cccagtcggc ccaacaatgg caaccttttg accagcatga gctgtcgcag agaagtcata
     8581 gtcttgaaca ttgacaccgt ccaccagaat ttctcctgct gacacgtcgt agaaacgtgg
     8641 aatcagattg accagagttg atttaccaga acctgttgac ccaataaagg ccactgtttg
     8701 accagtttct gctttaaagc taacatgttc aataactgcc tccgaatttg ccgcatagcg
     8761 gaaggtcaca tccttaaact cgacctgacc tttgaagttt tcatcagtca gctgcacttg
     8821 aacagggttt tggatagaag aatgcaaata taaaacttga ttaatccgct tagcagagac
     8881 catagttcgg ggaagaacga tgaagagtgc tcccatgaga aggaagccca tgacaaccta
     8941 catggcataa gacatgaaaa caatcatgtc actaaagaga ggcagacgcg ctatcggagc
     9001 agcgtcgtta atcacatagg ccccaatcca gtaaatcgcc acactcaaac cacttgaaat
     9061 ccccatcatg ataggattca aaatagccat aagacggttg acaaacaaat tcaaacgggt
     9121 caattcatca tttactgctg caaatttttc attttgataa tcctctgcat tgtaggcacg
     9181 aacgacacga atacctgtta aactctcacg agtgatactg ttcagtttat ctgtcagccc
     9241 ctgaatcaag gactgttttg gaaaggctag cgtcatcaaa acggtcgtca tcaggacgtt
     9301 gacaatcact gccacaagta cggcccagag ccagtattct gaatgaccta aaatcttccc
     9361 aatagcccag atagccataa ttggaccaca cgttaccact tgcaagccca tagtaatcaa
     9421 catttgaact tgagtaatgt cattggtagt acgcgtcaag aggctaggaa ttgaaaattt
     9481 cttaatctct gtctgcgagt aatccaaaac tcggttaaaa atatcacttc tcagcctact
     9541 agtataagaa gccgccactc gggatgcaaa aaatccaact gcaactacgg acaagaaggc
     9601 aagaaagagc attcccatca tcatgcttgc cggctgccac aactcatcta aattagttcc
     9661 ttgactacct agcaaatccg taattttcga gatataggtc ggcacttcca actctagata
     9721 gaccgaaaag caagtaaaga gaatggctag taaaatcatc cccattcttt tctactaatt
     9781 cttttggcta atttctttat tctctcctcc tattcccttg atatttgcct gtagttgacc
     9841 gagaactttc tcaaaaatca gtaattcatc ttcatcaatg tcttccatca actgcttgtc
     9901 tatgcgttca aaaaaagcct taacctgttg catctgagaa cgtgctttgt ccgtcagacg
     9961 aacaaactta gcccgcttat caacaggact cgcctccaat tccaccaaac cattttgcac
    10021 tatacgctta accagattac tagcaacaga cttggtaata ttgagttcct gctcgatatc
    10081 tttaatcaag accaagtctt ggtttttctc gcgattatcc aaaaaacgca caacctgacc
    10141 ttgcggccca cccataaatt caatgccgca acgtttggct tccttttgca ccatcaggtg
    10201 aatttgatga ccaaaacgct taaagactaa catcggttta cccataatct cccccttcta
    10261 aataaaaata gttctctgga gaataattaa atttctatga gaactatttt cttgattaaa
    10321 aaaatcccaa gtgattttct cacttaggat catgttctat aggttaaatt aaaacccatc
    10381 tacgttcgta taaatctttt ggacgtcttc gtcgtcttca agaacgctgt aaagtttttc
    10441 aaaggtttca aggtcttcgc ctgacaattc cacttctgac tgaggaatca tttccaattc
    10501 agtcacttgg aattcttcaa taccagactc acggagggca acgatagcct tgtgaaggtc
    10561 agttggcgct gtgtaaactg tgattgtacc ttcttgtgct tctacgtcat ccacatccac
    10621 atccgcttcg agcaattgct caaagactgc gtccgcatct tcacctccaa atacaataac
    10681 acctttgttg tcaaagaggt aagaaacaga acctgaagcg cccatgtttc cgccgttttt
    10741 accaaaggct gcacggacat tggctgctgt acggttgacg ttagaagtca aagtatccac
    10801 aattagcata gagccatttg gcccaaaacc ttcgtaacgt ccttctgtaa aggtttcgtc
    10861 tgtgtttcct ttggctttat caatcgcttt atcgataatg tgttttggca cttgggcttg
    10921 tttagcacgg tcgataacga atttcaaagc tgagtttgat tctggatctg gatcaccttt
    10981 tttagctgct acatagattt ctacaccaaa ttttgcatat actttagagt tggctccatc
    11041 tttagccgtt ttcttggcta cgatattggc ccatttacgt cccattagga atctcctttt
    11101 ttcacatttt aatctttctt attataacac aagttttttt gattttcact agaggaaatg
    11161 gattttatta gcaaatcaag ctaggatagc actttacctg ctaagatggt cttgcctttc
    11221 tatcttttta tcaacagaca ctcatccaca ttcaaaaaac aaactagacc attatctgca
    11281 aatagaaagt ttcagccaag tttgacaaag tcagctcaaa ttactgtttg aagtttgtag
    11341 atataagcga caaaaacaat catactgcac cttttgttga cagtctactc cagacatatc
    11401 atagttcaag taaatacttt gaaatccaac agttcttata ggcgctattg tattctaaga
    11461 aatcaataga agagtttcta agcaaatctc taatactcaa taaaaatcaa agagcaaact
    11521 agaaagctag cctcaggttg ctcaaaacac tgttttgagg ttgcggatag ggctgacatg
    11581 gtttgaagag attttcgaag agtataattt acgtgttccc aagatggaga agttagacta
    11641 gtacactggc acttctaaac cattgctagc aattgatttg ttcatattta atttcatttt
    11701 ttccataaat gggtattaga tataaacagc aaaatatttc cgatacgtgt cgttcttgaa
    11761 tttccaatca tctaaaacaa gtaaaggata atcaatcccc tgtatatcaa ggaattggct
    11821 acccttttta cttttttaca cattctgttt gatagattca ttttaacatc acgagcatac
    11881 tccaatggaa atcgctaggc aagagataaa ctttcagata tccgcagaga gatcatcgcc
    11941 tctttttgtc gcaagcattc tcctctccta gtcattttct accttatctt ctacctgcgg
    12001 atagagagtt gttccccaaa tagaaatcgt ccgcttacgc actagtggca aatcggtttt
    12061 ttcataaacc gtacgccacc attcccaagc aagcccggta cactctctaa ttttgacaga
    12121 gagattacga acattccctt ttaaaggaat actagtggta aagtgagccg ttaaatcctg
    12181 cccatttctg tcccaagcct taggagtcaa gacttcctta ccttgatgat cataggataa
    12241 ttcattccaa gtaatataat attgggcaac ataggcacca ctatgatcca gcagtaaatc
    12301 tccgtttctg taagctgtaa ccttagtctc aacatagtct gtactatttt gaaaggtcgc
    12361 aactacattg tcacgtaaaa aagaagttgt ataggaaatc ggcaagcctg gatgatctgc
    12421 tgtaaagcga ctgccttctt gaatcaagtc ctctaccata tccaccttgc ctgttacaac
    12481 tcgggcaccc gaacttgggt cgccccctaa aataaccgcc ttcacttctg tattgtccaa
    12541 aatctgcttc cactctgtct gaggagctac cttgactcct tttatcaaag cttcaaaagc
    12601 agcctctact tcatcactct tactcgtggt ttccaacttg agatagactt ggcgcccata
    12661 agcaacactc gaaatataga ccaaaggacg ctctgcagaa attcctctct gttttaaatc
    12721 ctctaccgtt acagtatctt gaaacacatc tcctggattt ttaacagcgt ctacgctgac
    12781 tgtataataa atctgcttaa aattaacaat ctgaatctgc ttttcgcctg aatggacaga
    12841 gttaaaatca atatcaagag aattccctgt cttttcaaag tcagaaccaa acttgacctt
    12901 gagttgttcc atgctgtgag ccgtgatttt ttcatactgc attctagctg ggacattatt
    12961 gacctgacca taatcttgat gccacttagc caacaaatcg tttaccgctc cgcgaacact
    13021 tgaattgctg gggtcttcca cttggagaaa gctatcgcta cttgccaaac caggcaaatc
    13081 aatactataa gtcatcggag cacgatcgac cgcaagaaga gtgggattat tctctaacaa
    13141 ggtctcatcc actacgagaa gtgctccagg atagaggcga ctgtcgttgg tagctgttac
    13201 agaaatatca cttgtatttg tcgacaagct ccgcttcttt ctttcgataa caacaaactc
    13261 atcgggtagc tgattaccct ctttgatgaa acgattttca atactttctc cctgatgggt
    13321 caagagtttc tttttatcgt aattcatagc tagtataaag tcatttactg ctttatttgc
    13381 catcttctac ctcctaataa gttcctggat tgagttgcat aaactcagac ttgttcagcg
    13441 aaatcagccg tggttggact aagtaatcca aaatttcctc gtacaattct tctgagacat
    13501 tgcgtcgccg tctggctaga taagaagtcg gaataaccgt attatccaac ataaatacct
    13561 tatctaagtc aatcaaggtt ggtcttgtaa aaggattacg agctagatcc ggctcttcta
    13621 tcataaagtt cttgaccaaa cgtctggtca agagagctgg tttgaaggtc tgatttttaa
    13681 ccaactcttt gtttttagtc atgctgttgt caatacagat atacatatga ttcttcacag
    13741 ccaaatcgct actaatagtc ggaaaaggca aataaagagc tacaacatct cctctcttaa
    13801 tcaagcaaga gcaccccctt ttctcctaat gtaacataga caggattgac caagtcttct
    13861 gattgactca gaatttccaa agtttgagtt tggcgcgctg tcaatttagt agcatcttgt
    13921 ctcttcaata caaaatgctt gtcgccaata accttgacaa tataatcctt ctccaaagct
    13981 gactggtaaa tccacatcag atgttgtctg tcctgagaac tcaagagaga aggattttca
    14041 agcctcccga tagtctgata aaaatcaaaa acaggagcta actcctgcca atctgattgg
    14101 ctagttgtca aggctagaaa aagggctttg cgagctgata cttcttggtt agccttgaga
    14161 gttactttcc cctccaagtt ttttagaaat cgggaaactc cagaaagcaa atttttctct
    14221 aactgcgaga aataaaaacc tttcgttccc agacataagt ctttcatgtc gctttctcta
    14281 gcaaataaga gctcaaacat ttgatagtaa aagaaaaata tctggcactg ggtcgcgctc
    14341 atcttttcct tatcggcttc tttttttaac cagagcaagg gcgacaggta gctggattga
    14401 gacatttcct ctacctccta ctctttttta actggagcat ctgcactagc tgccacttct
    14461 tttgactgga tactttccca ctggttaatc tcctctgaga taagaccttc gcatgtcttg
    14521 acaaataggg caaaagcctt ggtctttcct gcatatttct ccgtttggca ttgatagagg
    14581 aatttttctt tctccaggag ttgcgcagtt ttttggtaag aaatccaatt ttcctttgca
    14641 ttatacaaat tgataatccc ctcacacagc aagccgagac tggataaggc aaccgaaatc
    14701 aaacggtagc gatcacctgg cataggaata gcacaaaaga cagctatgag gaaacctgcc
    14761 acgatttctg ttatttttaa taccttatag cgcctacgat gttgaacgct tttctttaaa
    14821 aaatgagcta tctgtacgtc taatcgctct gtcaggtaca tttcttctgg cgtcatattc
    14881 gtaactcctt tcatttactt tgataatcat agtataacat atttgaatat caaagtatag
    14941 taaaaactgg actataactt attagacttc ctacgaaaca aaatatggta tagtagttct
    15001 atgaatgatg aagcaagtaa acaactaact gatgcacgat ttaagctcct tgttggtgtt
    15061 cagcgtacca cttttgaaga gatgttagct gtattaaaaa cagcttatca aaaaagtcgg
    15121 acaagctggt aaaatcttgg ctgacagtgg ttatcaaggg cccatgaaga tatatcctca
    15181 agcacaaact ccacgtaaat ccagcaaact caagtcgcta atagctgaag ataaagccta
    15241 taaccatgcg ctatctaagg agagaagcaa ggttgagaac atctttgcca aagtagaaac
    15301 gtttaaaatg ttttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa
    15361 tttgattgct ggtattatca atcatgaact aggattttgg tttcgcagga agtctattat
    15421 atatgcacag acatatgaaa tctttttgag atagcagtta tgatatattt atccattcac
    15481 aacatcttct gtactaatac tacaatattg gtacattttg attacacaat cgtttcaaaa
    15541 aatatgatgg aagcctgagt attttatagt aattcccttc ctatcaacta gatcaaccag
    15601 ttgagtgttg aatgttgaaa caccctagcc tcagcaaagg actatgatca gcatttccta
    15661 gccaatagga cgctccaaat cacctattct aagtccatca ctagcttggt cagtaagata
    15721 cagactgcaa ggcgagactg acgtggttga tgagattttg gaagagtatt acaatcatag
    15781 attcaccagt agacttgacc gcagatttaa ctatagtata ttgaaactag aatagtacac
    15841 ctctacttct aaaacattgt tagaaatcga tttgactgtc ctgatcaatt tgtcctgttc
    15901 ttttttcatt tcactatact aaaaaaccac ctgaaacaaa tcaagtgact agaggataaa
    15961 aacaaagact gggcaaaaag ttttgaaaag caaaaaacgc ataatatcag gtcttgaaaa
    16021 accttgacgt ttgattgtgg gaagattgac ttcattttct cctgaaattg aagttttttc
    16081 tagtttccag atatagacga attcaatata gccatctcgc tacaattatt ttaaccatcg
    16141 agaaaatttt tcagaatttc aaaaatttca ttttgatcca atgaaatgtc atcattttga
    16201 agaatagttg acttcacagg ctctaattct atcacaaagc ggtgtatgtc tgcttttacc
    16261 gcactacttg atgttatagc cgataacttt tcatctccta gcaaggaagc tgtcaaacgg
    16321 gtaatgtcat tcaaatgctt tttaatggac ttacttaaac cttgagcacc tgtagcagag
    16381 cgttccctca tatccagcca agctttagcc ttaaaaataa ttaatactca atgaaaatca
    16441 aagagcaaac taggaagcta gctgcaggct gctcaaaaca ctgttttgag gttgtagatg
    16501 gaagctgacg tggtttgaag agattttcga agagtataaa ccacaattac tcaataccga
    16561 atacccctga atggtttctt tttcatgcac caatatatta taataatctt catccaataa
    16621 taaggctgat aaactagcat cttggtcaaa atgtaaggga atttctcgac cgtccttctt
    16681 taatggatat tctggtaaga tactaaatag ttcaatcata gaaggaaact caggattagt
    16741 tgttgtaaat cgaaaaagct gcgctttctc atctttctga cttccttgat actctcccaa
    16801 ttctaaaaaa tgattcaagg tagtataaaa ttccttattt tttacttcat aaatgatgac
    16861 catatcataa tcttttgttg tgcgactttt aaatccttgc gaatccaata cgatagaggt
    16921 agcagttccc ccaatcagaa cataatagtt ctgaaaatcc gcaaacattt cttgaaaaat
    16981 aactttcgta ttagctggca tcatcttctc ccagatactg taatatcata ttttctagtg
    17041 cttcactctc ttcctctata cgtgggtcat catcatcttt taaggtcaaa taaagagaaa
    17101 tcggatctac aaattgttta tcatgattat ttttaaaatc attccaaaac tcagatacaa
    17161 aaggacgata tttccatatc tctagcatct ttccttttaa aacatgctga gaaagtggca
    17221 aggataactg attgaatttt ctctgccata tgacatagct agtattttca tccgtttcag
    17281 ctaaaaaagt tgaatgcgac aaagcataag caccaccata tagaaggtta gaaacagatt
    17341 ttatctgctt tatatcgcca tctggcaata aaatccgttt tttgatggga ttaaataaac
    17401 atgacacgga ttttaagaat aattctttct ttgacaccgt atatgtgtaa agcttatttt
    17461 gcttgtttaa ccaatataaa gctttaaaag tcctcaaaca cctataaatt gttgagtttg
    17521 gaagtccagt gacttgtgaa agcaaatcaa catctactac tttttgacct tttgtcaata
    17581 aaaaggcaat ccacgttaat tgttcgctag gtgttaattc cttagggact tcagtatcat
    17641 tcgcattcag tactagtccc aatggaggga agaagaggtt tcccttaaag tctacaaacg
    17701 gaactctagc ttgaagtaat tgcttttttt cactgtctga taacttcgaa aacaccaaaa
    17761 caacatccat attggctttt tcacccatag tgcgagcctg agtaacaaat gaactcaaac
    17821 tcccccttct cttttcttta atcaataaaa atgattcctt atgaaaagtc atttgagtat
    17881 actcaaacct ttttacaaaa ctgattggta aagtcaaagt cagttcctca atactgacat
    17941 caacataaaa ggattgaaaa attgacatta tctttttgac attcatgttt tttccttatt
    18001 ctatcatttt tattatattt tatcattcgg tataataaaa tataagtttt ttgataaaat
    18061 aaccaaaaac tactcgctat cccctctcac attaccctac atatcgtttg acatgcgact
    18121 gatagttcag gaaaacttca aggagaactt ttctctcatc cactatgcag gacttactat
    18181 ttcacttcta ctcctatagg ctcaatttga cacttttctt tttcgaattg ctcatatgcc
    18241 tcctgaatag tgagttcgat ttatccaaat ttatttttgt ctattttgat gaatacttcc
    18301 acgaagatag tctgaaaatg aatttgcacc aattttcctt ctctttttaa gcatattatt
    18361 ccagatatga ttgccctctt gttcagtaat aaattacgct ttaaacgctt caatcagtat
    18421 atctcctgtt gtcatatgtt ctaaagaaaa ttcttctaca tatggtttaa catctcttag
    18481 gttattactt cctaatatcc cattatgctt tttcgctaag gaaatagaag ccacttctcc
    18541 cttaccaata atcttgttac tatcatgatt tcttgttaaa tctctatata atgcgtattc
    18601 ttcagttcca atgtctatgc tcacaatctc agctgaaccc ttagttacca actggtctat
    18661 cctagatttt aaatggggaa ttgtaggtat attgatttca tcatacacct cttgtggaat
    18721 aacaatttta cccgaataga gcttttctaa aagatgttca gtaccaaccc ataaaaatac
    18781 tgaaatacaa tctgcatcaa taaatacacg actagtcaaa cgacaactcc cccctcttca
    18841 tctagcccat atacaatatc atatctgaaa gcatctagta acagttcctc atacttccct
    18901 tgcgaaattc tgttattttc taaaagttgt tcagttgaat taacatatta tcctaatatc
    18961 atttactttt tactttctga caaaggacga tataaacttg tatcatagcc taatcttgaa
    19021 gctgtctcta taacactaat atccatattt ttaatttctt ctgcatcaag gtatccatca
    19081 ttcctcaatc tatataacat aactttatga ctgataccat aaaactgacc caattttata
    19141 atatcttcta cttcaagatg agttctattg gcattttctc taatttcctc aaccatccta
    19201 tacagtgaag atgggaaaat taaaaaataa gaagcaaact gatccgcttt tctttcagtt
    19261 tcatctcctt caccaatcaa gataagactg actgaactct tcttcacctc atcataataa
    19321 agatgataca gttcatgtgc taaagaaaat ctttaccttc ctaatgacat gtctgaattg
    19381 actgcaatga gactgaactg agttccttta taacagactc cgctaatatt ctttccgagt
    19441 ccataaaata ccagcgtcaa attttctatc ttttgtacca atttaaaaat atctatcggc
    19501 gattcaccat cagctcccaa cttttttcta agatttgaag ctttattact taaatccatt
    19561 cgtgatattc ctttcacctt tttccatttt ggcaatcatg ctttgattca aagacaaata
    19621 ctcagcaatc ttatcttgag taagatttga agatattcta agttctttta ttctttttcc
    19681 aacatcacat acatttatca catacattta tcatattcat acacctctca taaaaagaat
    19741 agcacactct tgtcataatt ttttaaataa aaaaattatg acaaaacaag gaagcaattt
    19801 attgatgctg cttaaaaatc taaaattgat gagttcctat caaaaatcgt atcttcaacc
    19861 tcaaaacagt acttaaagct atccgactcg gtttacattg tcaaatttag attttatttg
    19921 agcataactt tctagtttgc tttttgattt ttgtttaata tagtagcaaa attgaaggaa
    19981 atatctccac aagaaagcgc atactattaa gctttttcaa gacctaataa tatgcgctgt
    20041 tctgatttga aagacattcc attattattt tactgtaatc aagccatctg gctctactgt
    20101 gaattctggc ttgtctgcca gtgttccgtc tggtttgagg tagtaccagc ctgttccgtc
    20161 cgctgactgg ataaaggcat ttgataccat ggcgccttct ttagcgtcta agtagtacca
    20221 agtgtccttg tacttgaccc agcctgtctt catggcacct tcttcgttga aatagtacca
    20281 cttatcagcg attttcttcc agcctgtagc catttcgcct gagttgtcga accagtacca
    20341 gttgccgtct gtgtgcttcc tccagcggtc tgcaagcata tagcctgaac tgtcaaagta
    20401 gtaccaagtg ccattgattt tctcaaactt gtcttttgga taagagccgt ctgaatgtac
    20461 gtaccagtag ccagtgtcat tcttctgcca gcctgtttca atcgtcaagc cgttctcaat
    20521 atcatgctta aactgctcac ggctaatgcc ccatttagca agatatggat aagggtcaac
    20581 gtggtctgag tggttgtttg gttggttatt cgtgcaatac tcgtgcgttt taattccagc
    20641 taaactccct gtatcaagcg ttttcggcaa acctgcttca tctgctagat tgcgtaagag
    20701 ttcgatataa aggcggtagt ccgtcatgaa ctcttctttg gttgaatggc tttcaatcag
    20761 ttcaaccgct gcataggtct cagcattcca accgccccca acgtcccagg caccattatc
    20821 aacaggtcct acctgcatga tgcaaccgtt cccaacaatg tgcgagaaaa aacctaattc
    20881 tgggtctttc cgccagtgat aatccgcttc attctgtacg gttgaatgcg gattcccagt
    20941 tgagtgtgcg tgtacttgcc tatatggttg cacgccgact tgaggcaaat ctgttcttaa
    21001 tttactcaca ttaatttcca tattctactc cttatcaatt aaaacaactc attttttaca
    21061 atccagaacc agaaactcct ttatttctac cttacaaaga agacaatctt agtcacgatt
    21121 aggcttgtag atagaacttc aaaacgcact attttgacac tgtaaatagg actgacaagg
    21181 tctgcattct atctacaata acaccccaga ctaaaaagct tttcaaaagt atatttttac
    21241 agtctctata tgtccttttc ataaatacta tactttatta tatcatataa aagaagtcaa
    21301 aagtctgtta aactattttc aacaccaaac taaagaagag aacacaagtt ttttcgattt
    21361 tcactagagg aaatggattt tattcagtaa atccaactag gattgcactt tggttgccaa
    21421 aattgccttt ccttctttta tcaagggatg acggaatagt gagaagtaca gttgaactgt
    21481 catggcaacc cagataagag gttcacaaag gataacaccc ttatatcctg cccaaggaat
    21541 aatcaaaacc acaaaaacga tttttccgat tagttcaata aagctagaaa ctagaggaag
    21601 gatcttctgc cccaagccct gcaagcaatt gcgataaatc aacaagaggc tcaaaatggg
    21661 ataaaaggct gaactgattt gcagatagag acttccattt tctatcaagt aaccatctgt
    21721 cgaactagcc aagaaggaaa ccaaggctgg actggcaaaa aagaggaaaa tacaaacaaa
    21781 aactgcccag gatatactta aacgactgcc gattcgaaga ccttgaacaa tgcggtcagg
    21841 tcgcttagct cctagattct gagaagcaaa ggtcgtcatt gatgcagaaa tagcggtcat
    21901 aggaagaagg gcaaaggtca taatgcgtcg agctgccgtc tgggcactaa taatcactgc
    21961 accaaatgta ttaacagaag actgtaaaat cacactgccg atagatacaa ttgaactcat
    22021 caagcccata gccaaaccct gctccaagag atccgcgtac aagcttttgt cccatttgaa
    22081 atgtttaaac tgtggcaaga gttctggcac acttttacga atataataaa agcagagaac
    22141 cgctgataaa ccttgcgaaa taatggtagc aagtcctgcg gattgaactc ccagatgcaa
    22201 ttgcgtaata aaatagagat ccagaaccac attaaccaag gcagagaaaa tcagaaatcc
    22261 cagggctgct agactgtcac caatagaccg caacaagcct gcaaaaagat tataagcaaa
    22321 gctgacacct acacaggtca caatcataga aatatattga taagattgag gaagaatttc
    22381 tgcaggagta tctaagtatt gcaagagagg atacaagcca agaaagccca gcaacataac
    22441 tagaatgctc aaaagagcac ctaaaatcca ggtggctgct actgcttcct tgattttagt
    22501 gaaattccga gccccataat aacgagcaat gacaatcccc atgccattgc caacaccaag
    22561 tgtaaaacct acaatcaggt caaaaatcgc tgtcgtcgct cctactgcag ccaaggattc
    22621 ttgaccaaga aatcgtccaa caatcaagac atcagcagtg ttatagagct gttgaaaaat
    22681 atttgatagc aagattggga aggcgaagct taagagcgag agaagaatcg gaccatgtat
    22741 caggtccact gttcgttttt tattcataaa gctattatat cagctttcca gaggagcgac
    22801 aagaaacagc aaacttatat atagcggaat gaaataagga cagtcaaatc gatttttaac
    22861 aatgttttag aagtagatgt gtactattct agtttcgatc tattatagtt ggttgagttt
    22921 ggaatagtac gctgtagctg ctaaaacatt tctagaaatt aatttgactt tcctaataaa
    22981 gttgttcata tcttatttca agctactata tataatctaa aaacttgaat caaaaatcga
    23041 aaaagtagcg gatttctcca ctgctttaac agcttattct tcaatttcga tttcaagttc
    23101 atcgcctaag tcaagcgtaa cttcttcatt cactgcttct tctttctttg gctcatcttt
    23161 tttgttttca gtatcttgtt ctgaaacttc ttctccatca atcaagccaa atttagaacg
    23221 gacttgctta tcaatttcat caaagatttc tgggtgctct gccaagtatt tcttagcatt
    23281 ctcagaacct tgcccaattt tttcatcttt gtaagaatac caagcccctg cttttttgat
    23341 aatatccaaa tcgcttgcaa tcttcaaaag ctcaccagtc ttagaaattc cttctccgta
    23401 cataatttca actacggctt ccttaaacgg tggagctacc ttatttttta caaccttaat
    23461 cttagtttct ttaccgacat tggtttcttt ttgatcacca gttcccttaa tttgtgtatt
    23521 accacgaaca tccaagcgga ctgaagcata gaatttcaaa gcacgtccgc ccggtgttgt
    23581 ttctggattt ccaaacatca ctccaacttt ttcacgcaat tggttgataa aaatggcaat
    23641 tgttttggtt ttattgatag aggcgccaag tttacgcatg gcctggctca tcatacgagc
    23701 ctgcaaacca acatggctat ctccgatatc tccatcaatt tccgcacgag gaacaagggc
    23761 agcaactgag tcgactacga caagatcaac tgcacctgag tcaatcaatt ttcccgcaat
    23821 ttcaagacct tgctctcctg agtctggttg agacaagagc aattcgtcaa tattgacacc
    23881 aagggccgca gcataagctg gatcaagggc atgttccgca tcgataaagg cagcaatccc
    23941 accttctttt tgcgcttgtg caactgcatg aagggcaacc gttgtcttac cagatgactc
    24001 tgggccatag atttcgatga tacgtccctt aggataacca cctgagccaa gggcaatgtc
    24061 aagagctaaa gaacctgagc tcatcacttg caccttttgc tccgcacgtt cacccaaacg
    24121 catgattgat cctttaccaa agtctttctc aatcaattta agagcgtcat tcaaggcctt
    24181 ttcacgttct gccccaaatt tttttgaaat ttcttctaat ttttttggtt ttttcgccat
    24241 tctattctcc tacattctaa tgatcctcag acctatctac tattatatca aaagttagtc
    24301 acttaataaa gccttgcgaa ctaggttaaa ggcatgcata accgcaatgt gacgtacatc
    24361 tgctcggctt ctgcctccaa tattaacctt gataacctca gttccttgat cttgcgccaa
    24421 gcctatgaag actgtcccaa ctgggtgtcc ttctaggcta tctggtcctg ccactccagt
    24481 caaactaatt ccaaaatcag actgggtctt gcttcgtgcc tgctcagcca tcttctgagc
    24541 tgtaaattca gacaccacac catgttcttc caaattcttg gcaggaatat ccaacatcct
    24601 tgatttttcc tccaagctat aggtcacaaa accaccctca aatatacttg aaactccaga
    24661 aaaattcgcc acggtagctt ggaaaagacc tgccgtcaaa ctctctgcag ccgcgatggt
    24721 tttcccttgc cttttcagtt cttctaccac aatgctggct aaactagttt cttccccata
    24781 accatagcaa aagtctcgta aagaaattcc ttcgaaagtc tggcagtcca agatttgatt
    24841 ttccaagata tccagcgctt gattcgcctc ttcttgactg ctagcctttg ttgacagacg
    24901 tagagtgact tctcctgtct tggcataagg ggccaaggta ggatcgatct gattatcaat
    24961 taaatcagcc aaaatcgtaa ccaactggct ctcgccaatc ccaaagaaac gaagaactcg
    25021 ggaatacagc ttgctccctg tcatcaactt gggtagaagt tggtttaaga ccatgggttt
    25081 caattcactt ggcggacctg gaaggacgac ataggtcact ccgtctactt ctaattttcc
    25141 tccaacagcc agtcctgttt cgtttggcag tggaatcgct ccttctacaa tttgagcttg
    25201 tctttcgtta tttggtgttc gggcatagtc tggtcgcagg gtaaaaaaga tatccaactt
    25261 ctcctgagcc tgaggatcaa agactaatgc tttccctaaa aatttagcta gggtttgttt
    25321 ggttaggtcg tcctcagttg gccccaaacc gcctgtcaaa atcaccagac tgctacgttg
    25381 actggcaatc tcaagcaaag acaagagacg aacttcattg tctcctacag ccgtctgaaa
    25441 atatacatct accccaatct cagctagttt ttccgacaaa aactgggcat tggtgttgac
    25501 aatctgtcct gtcaaaatct ctgttccaac agcaatgatt tctgctttca tgtttcctcc
    25561 tacctatcta ttcgtatttt tttgaaaaaa tcgcaggaat tttcctacga ttgatttttt
    25621 atttgtatca aaagttaatt atcttcatca ccaacaggtg ctctgccaaa taaatcttca
    25681 aataaaaccg cattggtttc aagctgagta acttcttctt gtcccaaaga acgtcggagt
    25741 atattttgca tttccaacat atgtgctctc gaaacaatct ggtaagaaac accttgaagt
    25801 atctctcctt caccctgcaa ctgctgagtt tcaatggttt taaatgaatc tttatagcct
    25861 agcaagttag ggatactttt tgcagacaaa tcaatattgg tctgcatatt gtcactcaaa
    25921 gcttttagaa tctcttgata atgaccaacg ctatttaaac tgagagcttt ttccatgact
    25981 ttttgaataa cttcacgttg acgtttttga cgaccataat ccccctcagg atcttggtaa
    26041 cgcattcgtg catagactag ggcttcttct cccccaatat gttgctcccc aacaccgata
    26101 gaaatagtat taaattcttc ttggtcactg atagaaattg ggaaacctag gatattattg
    26161 actgtaatac ctcctactgc atccactagt ttttgcaacc ctctcatatt gaccatcaca
    26221 tagcgatcaa tatggatatt catcattttt tgaatggttt ctatagcaag ctctgctcca
    26281 ccatctgcat atgctgagtt cagtttcgct tcatgagcct gaccattccc tgattcaatg
    26341 cgcgtcagaa tatcccgctc taaactcatc attgttgttt ttttcgtttt aggattcact
    26401 gtcatcaaga tcatgctatc acttctaccg acccaagttt cagttcgttc agcatttccg
    26461 gtgtccactc ccattaacag aatggttaga ggttcagtcg cttcaataac cttggtttct
    26521 tcgccgattt ttttataggt tttagctaag gtttctgtcc cttgttgata aatagtataa
    26581 gcaaaaacac ctactcctac tacagttaca gaaagtaaag ctagcaccat tccaataatt
    26641 tttttaacca tatttctact aacctatcag tttacccatc aagtaaacat cgataaattt
    26701 cccttcttct atatatgccc cacgctcttg gctaccttca atgacaaagc catgcttttg
    26761 ataaagatgg actgctgctt gattacgagt ttggacagtc agttggagac gacgcagaat
    26821 gccacttgct tgtgcccact ctgtcgcttc ttctagcaac aaacttccca agccattatt
    26881 ccaatatctt tttccaatca caatgaagag atctccaata tgacggactc tcttacgctg
    26941 atcagctgta atatttacaa taccagcaat tttgccattt aagaatgcaa gtaaggttat
    27001 ctgattgtcc gaactagctt gcttgttgag gaatatttcc atctcctcac tagtcaagag
    27061 aataccatct ccgtctaggc tggtaaagtc tgtctccaaa ctcacacgat ttaaaaaggc
    27121 cactaattca gctgcatctt tgggctctgc ttccctaatg agcaattcat actccatatt
    27181 gaagctcctc taacaatttc tcagcacgca aaccctttgc ctgaaaattt aaacggcgtc
    27241 catctgcttc ttttagaatt tccaattcta aataagcatc tggcaaggca tctcctaaga
    27301 gatttcccca ctcaataaca gtcacgccgc caccaaagat aaactcatcc aagtcgatag
    27361 aatcagcatc tccttcaata cgataaacat ctaggtgata aagtggaagt cgaccttcat
    27421 actctctcac gatagtatag gtgggacttt taatcatttg agaaatctgt aatccttttg
    27481 caagtccttt agtaaaggtc gttttacctg cacccagttc tccagttaag attacaacat
    27541 cattctttgc taatagatgg cccaaacgct cccctaaggc ttgcaactct tcttcatttt
    27601 ttgtgtacat actcttatta taccaaaaac ttttcttttg tgtctatttt cctactaaac
    27661 ttatcatcat aacatccata aaaaacagac tttctctaaa agaaaatgag cgtaacaatg
    27721 accaatacaa gatctcggaa aatatgacca taaaaggaaa cttccttctt aaccgaattt
    27781 gggacaagat aggctgcaaa aaacaagccc agtccaatat aaatcagaag tgagacaatg
    27841 gtcattggat ttcttaagaa aagaagtgtt gctaaaatag tcaccaacac tgtctttttt
    27901 ctgtctagca tagcaagaaa atcgcgcacg tattttttca agggtaaaaa aatcagcaaa
    27961 tctagcccaa ataggaaaaa gaaggatggc aataaaaagt caactaattc ttgctgcagc
    28021 gtatttttga tgaacaagtt atctgacaaa acaagaacag ctcctaacaa attaattaag
    28081 agtaacatac tgtaaaaaag cttcaccgac ttcttactgg ctaggacact atggacttct
    28141 tgcttacggg tataaagata atttactcca gcacagattc ctgaaacgaa aaccatgctt
    28201 ccgatgaaaa aagctgtact ttgtttaaag gacaagatgc attccttcca taagaaacag
    28261 ctactcaaac tgatttgaat taaagctaac aaaaataaga ttctcattga tttcatcttc
    28321 tctctccctt cctaccaatc attatactag gagaaaagag agaactgttt ctaatcttct
    28381 caaatgtctc tttaagacgc taaacaaaca ctagagacta atactcaatg aaaatcaaag
    28441 atcaaactag gtagctagcc acaggttgct caaaacactg ttttgagatt gcagatagag
    28501 ctgacgtgat ttgaagagat tttcgaagaa tataaatttg aaatcatgaa aatccgtcaa
    28561 acgggtggtt gttttgtctc gcacctcacg gagcgagacg gactcagagt cacataatta
    28621 taaggctgat agtattaatc taactatcag ccttacaggt tatttaacgt ttcagaaaaa
    28681 ctataatgtc aagattaact aaacagtatc tagttccttc aaataatttt ctatcttcat
    28741 caacattaaa ggattgttat aaatcttaca taactctctt gcttctatat aataattttt
    28801 gacttgttct ctgtctagaa atttggctcc agcatttcct acaagaataa gtagaggagc
    28861 caattggtag cttgtctgtc tttgtttaca gagttcaatc gtttcaagag cttcttggat
    28921 ggcttcatta tatttttcct ttgatactag gtagtgagcg tagttgtaac gaactctgat
    28981 gtagccaaat aaaaactctt gatggtccaa attttttgtc tgatacaact ctattaaatg
    29041 agagtagttt gcctcatatt cttgttcacg acccactaag gaatagaaat tagatagagt
    29101 attcaacgcc tttaaataaa tcagagtatt tgaagagact tttaataata tattttccaa
    29161 tgaagaaatt gcctcacact tactgtcata ttgatagaag tcaataatag cttgaatcca
    29221 ttcaaggtaa gttcggtctt ctagtgttag aaaagtactt cgttcaatct ctattctata
    29281 aatatattcc aaatcgtcat aatttctatc gtctaatagt cgagcagaca attgcttgaa
    29341 attagagagg ttagatttaa tttcaatttg ttcattaaaa aaataatcta atggtacttc
    29401 aagtcgttga gagagtttga acaaaaggtc tgcggaggga atgaaatgcc ctctctcaat
    29461 tttactaatc tggctttgtt cacaaattcc ttctgcaaga gtttgttggg agagtctcaa
    29521 ctcttttctt ttcaatctga atttctctgc gagtgtactc attaaagata ataaaccttc
    29581 ctatttgctt aattgcatta taaaattttt agttcaaaat agcaagttaa agaaataaat
    29641 aatt
//
LOCUS       DAVKLX010000026        29254 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKLX010000026 DAVKLX010000000
VERSION     DAVKLX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.026"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..362
                     /locus_tag="VW325_001208"
     CDS             <1..362
                     /locus_tag="VW325_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734617.1"
                     /translation="RKINYIIQNREIIYFYSQVKVVDKLTDSESKKYGEYIDLYESCR
                     QNNLSVLLNFLQNSDDKRGVIASNSVRIMFESLDKADQIAALNICRNYVARENVLNKE
                     DAEKLLNEIDKKLEISR"
     gene            418..1113
                     /locus_tag="VW325_001209"
     CDS             418..1113
                     /locus_tag="VW325_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9734618.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYIFEELNYRRYEWKCDAL
                     NLPSRRVAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDMDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            1125..1541
                     /locus_tag="VW325_001210"
     CDS             1125..1541
                     /locus_tag="VW325_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9734619.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEHALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPIYQRQGIGSALMKEALKDYKDAYQVQ
                     LVTEETEKNVGFYRSMGFEILSTYNCIGMTWANREK"
     gene            complement(2356..2448)
                     /locus_tag="VW325_001211"
     CDS             complement(2356..2448)
                     /locus_tag="VW325_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9734620.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            2609..3580
                     /gene="ruvB"
                     /locus_tag="VW325_001212"
     CDS             2609..3580
                     /gene="ruvB"
                     /locus_tag="VW325_001212"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9734621.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            3669..4133
                     /locus_tag="VW325_001213"
     CDS             3669..4133
                     /locus_tag="VW325_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU9734622.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQVYMHQHSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            4364..5122
                     /locus_tag="VW325_001214"
     CDS             4364..5122
                     /locus_tag="VW325_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9734623.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            5131..5934
                     /locus_tag="VW325_001215"
     CDS             5131..5934
                     /locus_tag="VW325_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9734624.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            5956..7215
                     /gene="rseP"
                     /locus_tag="VW325_001216"
     CDS             5956..7215
                     /gene="rseP"
                     /locus_tag="VW325_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9734625.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            7228..9081
                     /locus_tag="VW325_001217"
     CDS             7228..9081
                     /locus_tag="VW325_001217"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9734626.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            9181..10560
                     /gene="bglA"
                     /locus_tag="VW325_001218"
     CDS             9181..10560
                     /gene="bglA"
                     /locus_tag="VW325_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9734627.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            10752..12560
                     /gene="glmS"
                     /locus_tag="VW325_001219"
     CDS             10752..12560
                     /gene="glmS"
                     /locus_tag="VW325_001219"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9734628.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYSMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            12679..13728
                     /locus_tag="VW325_001220"
     CDS             12679..13728
                     /locus_tag="VW325_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9734629.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(13828..17625)
                     /gene="spuA"
                     /locus_tag="VW325_001221"
     CDS             complement(13828..17625)
                     /gene="spuA"
                     /locus_tag="VW325_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9734630.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYTSSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTIHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            18042..18455
                     /gene="rpsL"
                     /locus_tag="VW325_001222"
     CDS             18042..18455
                     /gene="rpsL"
                     /locus_tag="VW325_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9734631.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            18475..18945
                     /gene="rpsG"
                     /locus_tag="VW325_001223"
     CDS             18475..18945
                     /gene="rpsG"
                     /locus_tag="VW325_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9734632.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            19370..21451
                     /gene="fusA"
                     /locus_tag="VW325_001224"
     CDS             19370..21451
                     /gene="fusA"
                     /locus_tag="VW325_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9734633.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPVGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            21555..25946
                     /locus_tag="VW325_001225"
     CDS             21555..25946
                     /locus_tag="VW325_001225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9734634.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLIMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            26048..26311
                     /locus_tag="VW325_001226"
     CDS             26048..26311
                     /locus_tag="VW325_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9734635.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            26304..26582
                     /locus_tag="VW325_001227"
     CDS             26304..26582
                     /locus_tag="VW325_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9734636.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            26603..26755
                     /locus_tag="VW325_001228"
     CDS             26603..26755
                     /locus_tag="VW325_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734637.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            26777..28018
                     /locus_tag="VW325_001229"
     CDS             26777..28018
                     /locus_tag="VW325_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9734638.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            28028..28258
                     /locus_tag="VW325_001230"
     CDS             28028..28258
                     /locus_tag="VW325_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9734639.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            28526..29248
                     /locus_tag="VW325_001231"
     CDS             28526..29248
                     /locus_tag="VW325_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9734640.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
BASE COUNT         8297 a         5487 c         6915 g         8555 t
ORIGIN      
        1 tcagaaaaat aaattatatt attcaaaata gggaaataat ctatttttat tcgcaggtaa
       61 aagttgtaga taaacttact gattctgaat cgaaaaaata cggtgaatat attgatttgt
      121 acgaatcgtg tcgtcagaac aatttaagtg ttctacttaa ctttcttcaa aattcggatg
      181 ataaacgagg agttattgct agtaactcag tgagaattat gtttgaatct ttggataaag
      241 cagaccaaat tgcggccctg aatatttgta gaaactatgt agcacgagaa aatgttttaa
      301 ataaagaaga tgcagaaaaa ttgttaaatg aaattgataa aaaacttgaa attagcagat
      361 aggacaattc aaatagagtt gagtaaaaag tttgaaaagt gaaacaagga gaaagctatg
      421 ccagtaaatg aatatggtca gatgattggt gagtcaatgg aaggttatac acccggtgca
      481 ctgccttcta ttgatttctt agaagggcgt tatgctagga tagaggctct ctcggtagaa
      541 aaacatgcgg aggatttgct agctgtttat ggcccggata ctcctcggga gatgtggacc
      601 tatctttttc aggagccagt agcagatatg ggggagctgg ttagcctttt aaatcagatg
      661 ttggctcgta aggaccgttt ttactatgca atcatagaca aggcgactgg taaggctttg
      721 ggaacttttt ctctcatgcg cattgaccag aataaccgag taatagaagt cggagcagtc
      781 actttttctc cagagctaag gggtacacgg ataggaactg aggctcagta tctcctagct
      841 cgctatattt ttgaggagct aaactatcgt cgctacgagt ggaaatgcga tgccttaaat
      901 ctgccatcca gacgagttgc ggagcgtttg ggatttgtct atgaaggaac cttccgccag
      961 gcggtggttt ataaggggcg tacgagagat acggattggt tgtctatgat tgatatggac
     1021 tggcctcaag tcaaagatcg tttggaaaca tggctgcgtc ctgaaaattt tgataaaaat
     1081 ggacagcagt acaagagctt gagagaactt taagaggtgt tgagatgatt actattaaaa
     1141 agcaagaaat tgtcaagcta gaggatgttt tgcatctcta tcaggctgtc ggttggacaa
     1201 attataccca tcaaccagag atgctggagc atgccttatc gcattcatta gtaatttatc
     1261 tggcacttga tggtgatgct gtggtgggct tgattcgttt ggttggagat ggattctcat
     1321 cagtttttgt acaggatttg attgttttac caatctatca gcgtcaaggg attggtagtg
     1381 ccctaatgaa agaggcctta aaagattaca aagatgctta tcaagtccag ctggtgacag
     1441 aagagacaga aaaaaacgtg ggattttatc gttctatggg ctttgaaatc ttatccacct
     1501 ataattgtat agggatgact tgggcgaatc gagaaaaata acaaaacttg ttttttctta
     1561 agcaaaattt aaggatggtc tagtattata tagtcattaa ataaagacct cctaacttta
     1621 tttaataaaa tcctaaactt ttttcatcac aatctcctaa tgaagccacc caatcaggtg
     1681 gcttttttgc gtggtgaggt gctggtgata gaaatttttt gcaaaatagt aaaattttag
     1741 aaaagttaag ctagttttaa gtttcgtctt gtatcatgta gttattaaat aaagacctcc
     1801 taactttatt taataaaatc ctaaactttt ctttttcata ataatctccc ttaactccac
     1861 ccaatcaggt ggagtttttt agctctattt caggcttttg gggactattc taaaaataat
     1921 ttttcgatat ttttcggtat ttttcggatt ttggtcgggg aattggcggg gactttttta
     1981 gcgaatataa ctaagaaata ggtctgtggt cgcttcagct agttcaaact caatttgatt
     2041 gtaagtgact gttaattgaa gaactggaac tactgtaata atgattttgc ttgtctagta
     2101 gtggcgtatt aaatgagaag tgacatgtaa gattgtctct tcgtttacga aatcaaaatt
     2161 tctatgaaat tgatagaatc cctaacgttc tcttaatctt aaccgtcttg tcgtacagcg
     2221 gttgaagaaa atgtaaacct ttgacaaaat tcgtgaacta tgggataata aaaaggaatt
     2281 gagtactagt tctatatcat aggctagaaa agaccccaag ctcacgacca aataagcttg
     2341 gggtcttttt tgacactatt tgtccttgtt tagccattta tcgactatac gaagaacgac
     2401 accgaccaca attggtccga tgatagtttt gagtactaat tccatcatgg gctatctcac
     2461 ctcctttcgt aggcggtgta gcagtgtcgt agaatattat accacataag tgctaaactt
     2521 aggagtcacc caatcaggtg gcttttttgt ttgtggcttg gttttttgat ataatagagc
     2581 catgagtaga attttagata atgagataat gggggatgag gagttagtag aacgcacgct
     2641 ccgtcctcag tatttacgtg aatatattgg gcaggataag gttaaggacc agcttcaaat
     2701 ctttatagaa gctgccaaaa tgcgagatga agcgctggat catgtcctct tatttgggcc
     2761 tccaggtttg gggaaaacaa ccatggcttt tgtcattgcc aatgaactgg gagtcaatct
     2821 caagcaaacg tctggtcctg ttatcgaaaa agcgggtgat ttggtagcga ttttgaatga
     2881 gttagagcct ggagatgtcc tctttattga cgagattcat cgcttaccca tgtcagtgga
     2941 agaagtgctt tatagtgcca tggaggactt ctacatcgat attatgattg gggctggtga
     3001 aggcagtcgc agtgttcatt tggagttgcc tccttttacc ttgattggtg cgacgactcg
     3061 ggctggtatg ctctcaaatc cactacgggc acgttttggg attacaggtc atatggagta
     3121 ttatgcccat gctgacttga cagaaattgt cgagcggacg gcagatattt ttgagatgga
     3181 aatcactcat gaggcagcat ctgagttggc cctacgtagt cgtggaaccc ctcgtattgc
     3241 caatcgtctc ctcaagcgcg tgcgcgattt tgcccagata atggggaatg gggtaatcga
     3301 tgatattatt accgataagg ctttgactat gctggatgtt gaccatgaag gtttggacta
     3361 tgtggatcaa aaaatccttc gtaccatgat tgagatgtac agtggaggac ctgttggtct
     3421 aggaactctt tctgtcaata tcgccgaaga gcgtgagaca gttgaagaca tgtatgagcc
     3481 ttacttgatt caaaaaggtt ttatcatgcg gacacggtct ggacgggtgg cgactgctaa
     3541 ggcatatgag cacttaggtt atgaatacag tgaaaaataa gcaagaaatc ttagaggctt
     3601 ttagagaaaa tccagatatg atggccattc tgacgatcat ccgagacctt ggtttgaaag
     3661 actcgtagtt ggcagcaggt tctgtcagaa atttcatctg gaatcttttg tcagacaaat
     3721 ccccttttga tcatgaaaca gatatagatg tgattttctt tgatccagat ttttcttatg
     3781 aggaaacctt attactggag aaaaagctga gagaggattt tcctcagtac cagtgggaat
     3841 tgaaaaatca ggtctatatg catcagcaca gtcctcacac tgcttcctat accagttctc
     3901 gtgatgctat gagtaagtat ccagaacggt gtacggcagt tggactgcgc ttgaatgaag
     3961 aatcagattt tgaactctat gcaccttatg gtttggagga tattttgaat tttcaagttc
     4021 gtccaactcc tcatttctta gaaaatgaag accgaatgga actctatcaa acacgtttat
     4081 ccaagaaaaa ttggcaggag aaatggaaaa atttgatttt taaaaatact taaggaaact
     4141 ttaagctagg gagtgtacac taagttcata agttaagaag accttaactt aaactcctaa
     4201 aactttttca taataatctc cctataaaaa taaagtcgcc caatcaggcg gcttattttt
     4261 ttgaaaaatg ggcatggtgc ctgagaataa atagcttagt gatagaagaa aatggggaaa
     4321 tatggtataa tgaaacgata gatttttgaa taggaataag atcatgtttg gattttttaa
     4381 gaaagataag gctgtggaag tagaggttcc gacacaggtt cctgctcata tcggcatcat
     4441 catggatggc aatggccgtt gggctaaaaa acgtatgcaa ccgcgagttt ttggacataa
     4501 ggcgggcatg gaagcattgc aaaccgtgac caaggcagcc aacaaactgg gcgtcaaggt
     4561 tattacggtc tatgcttttt ctacggaaaa ctggacccgt ccagatcagg aagtcaagtt
     4621 tatcatgaac ttgccagtag agttttatga taattatgtc ccggaactac atgcgaataa
     4681 tgttaagatt caaatgattg gggagacaga ccgcctgcct aagcaaacct ttgaagcttt
     4741 aaccaaggct gaggaattga ctaagaacaa cacaggattg attcttaatt ttgctcttaa
     4801 ctatggtgga cgtgctgaga ttacacaggc gcttaagttg atttcccagg atgttttaga
     4861 tgccaaaatc aacccaggtg acatcacaga ggaattgatt ggtaactatc tctttactca
     4921 gcatttgcct aaggacttac gagacccaga cttgattatc cgtactagtg gagaattacg
     4981 tttgagcaat ttccttccat ggcagggagc ctatagtgag ctttatttta cggacacctt
     5041 atggcctgat tttgacgaag cggccttgca ggaagctatt cttgcctata atcgtcgtca
     5101 tcgccgattt ggaggagttt aggaggaaat atgacccagg atttacagaa aagaaccttg
     5161 tttgcaggga ttgccctggc tattttccta ccaattttaa tgattggggg cctcttgctt
     5221 cagatagcaa tcggaatcat agccatgcta gccatgcatg aacttttgaa gatgagaggt
     5281 ctagagacca tgacgatgga gggcctcttg accctctttg caacctttgc attgaccatt
     5341 cccttggaga attacctgac ttttttgcca gttgatggga atgtggttgc ctatagtgtt
     5401 ttgatttcaa tcatgttagg aacgaccgtt tttagcaagt cttatacgat tgaggatgcg
     5461 gttttccctc ttgctatgag cttctacgtg ggctttggat ttaatgcttt actagatgct
     5521 cgtgttgcag gtttggacaa ggctctctta gccttgtgta tcgtctgggc gacagacagt
     5581 ggtgcctatc ttgttgggat gaactatggg aaacgaaagt tagcaccaag ggtatcgcct
     5641 aataaaaccc ttgagggtgc cttgggtggt attttaggag caattttagt aaccattatc
     5701 tttatgatag ttgacagtac agttgctctt ccatatggaa tttacaagat gtcagtcttt
     5761 gctattttct ttagcattgc tggacaattt ggtgatttac tagaaagttc gatcaaacgt
     5821 cattttggtg ttaaggattc tgggaaattt atccctggac atggtggtgt tttggatcgt
     5881 ttcgatagta tgttgcttgt atttccaatc atgcacttat ttggactctt ttaatcaaaa
     5941 gacggaggaa acgctatgct cggaatttta acctttattc tggtttttgg gattattgta
     6001 gtggtgcacg agttcgggca cttctacttt gccaagaaat cagggatttt agtacgtgaa
     6061 tttgccatcg gtatgggacc taaaatcttt gctcacattg gcaaggatgg aacggcctat
     6121 accattcgaa tcttgcctct gggtggctat gtccgcatgg ccggttgggg tgatgataca
     6181 actgaaatca agacaggaac gcctgttagt ttgacacttg ctgatgatgg taaggttaaa
     6241 cgcatcaatc tctcaggtaa aaaattggat caaacagccc tccctatgca ggtgacccag
     6301 tttgattttg aagacaagct ctttatcaaa ggattggttc tggaagaaga aaaaacattt
     6361 gcagtggatc acgatgcaac ggttgtggaa gcagatggta ctgaggttcg gattgcacct
     6421 ttagatgttc aatatcaaaa tgcgactatc tggggcaaac tgattaccaa ttttgcaggt
     6481 cctatgaaca attttatctt aggtgtcgtt gttttttggg ttttaatctt tatgcagggt
     6541 ggtgtcagag atgttgatac caatcagttc catatcatgc cccaaggtgc cttggccaag
     6601 gtaggagtac cagaaacggc acaaattacc aagattggct cacatgaggt tagcaactgg
     6661 gaaagcttga tccaagctgt ggaaacagaa accaaagata agacggcacc gactttggat
     6721 gtgactattt ctgaaaaggg gagtgacaaa caagtcactg ttacacccga agatagtcaa
     6781 ggtcgttacc ttctaggtgt tcaaccgggg gttaagtcag attttctatc catgtttgta
     6841 ggtggtttta caactgctgc tgactcagct ctccgaattc tctcagctct gaaaaatctg
     6901 attttccaac cggatttgaa caagttgggt ggacctgttg ctatctttaa ggcaagtagt
     6961 gatgctgcta aaaatggaat tgaaaatatc ttgtacttct tggcaatgat ttccatcaat
     7021 attgggattt ttaatcttat tccgattcca gccttggatg gtggtaagat tgtgctcaat
     7081 atcctagaag ccatccgccg caaaccattg aaacaagaaa ttgaaaccta tgtcaccttg
     7141 gccggagtgg tcatcatggt tgtcttgatg attgctgtga cttggaatga cattatgcga
     7201 ctctttttta gataatcgag gaatattatg aaacaaagta aaatgcctat cccaacgctt
     7261 cgcgaaatgc caagcgatgc tcaagttatc agccatgctc ttatgttgcg tgctggttat
     7321 gttcgccaag tttcagcagg tgtttattct tatctaccac ttgccaaccg tgtgattgaa
     7381 aaagctaaaa acatcatgcg ccaagaattc gaaaagattg gtgctgttga gatgttggct
     7441 ccagcccttc ttagtgcaga attgtggcgt gaatcaggtc gttacgaaac ctatggtgaa
     7501 gacctttaca aactgaaaaa ccgtgaaaaa tcagacttta tcttaggtcc aactcacgaa
     7561 gaaaccttta cagctattgt ccgtgattct gttaaatctt acaagcaatt gccactcaac
     7621 ctttatcaaa ttcagcccaa gtatcgtgat gaaaaacgcc cacgtaatgg acttcttcgt
     7681 acacgtgagt ttatcatgaa ggatgcttat agtttccacg ctaactatga tagtttggat
     7741 agtgtttatg atgagtacaa agcagcctat gagcgtattt tcactcgtag tggtttagac
     7801 ttcaaggcta ttattggtga cggtggagcc atgggtggta aggatagcca agaatttatg
     7861 gccattacat ctgctcgtac agaccttgac cgctgggttg tcttggacaa gtcagttgcc
     7921 tcatttgacg aaattcctgc agaagtgcaa gaagaaatca aggcagaatt gctcaaatgg
     7981 atagtctctg gtgaagatac cattgcttac tcaagtgagt ctagctatgc agctaactta
     8041 gaaatggcaa caaacgagta caaaccaagc aaccgtgttg tcgctgaaga agaagttact
     8101 cgtgttgcaa cgccagatgt taaatcaatt gatgaagttg cagctttcct caatgttcca
     8161 gaagaacaaa cgattaaaac cctcttctac attgcagatg gtgagcttgt tgcagccctt
     8221 ctagttggaa atgaccaact caacgaagtc aagttgaaaa atcacttggg agcagatttc
     8281 tttgacgttg ctagcgaaga agaagtggcg aatgttgttc aagcaggatt tggttcactt
     8341 ggaccagttg gtttgccaga gaatattaaa attattgcag atcgtaaggt gcaagatgtt
     8401 cgcaatgcag ttgtcggtgc taacgaagat ggctaccact tgactggtgt gaacccaggc
     8461 cgtgatttta ctgcagaata tgtggatatc cgtgaagttc gtgagggtga aatttcccca
     8521 gatggacaag gtgtccttaa ctttgcgcgt ggtattgaga tcggtcatat tttcaaactc
     8581 ggaactcgct actcagcaag catgggagca gatgtcttgg atgaaaatgg tcgtgctgtg
     8641 ccaatcatca tgggatgtta cggtatcggt gtcagccgtc ttctttcagc agtgatggag
     8701 caacacgctc gcctctttgt taacaaaacg ccaaaaggtg aataccgtta cgcttgggga
     8761 atcaatttcc ctaaagaatt ggcaccattt gatgtgcatt tgattactgt caatgtcaag
     8821 gatgaagaag cgcaagcctt gacagaaaaa cttgaagcaa gcttgatggg agctggttat
     8881 gaagtcttga cagatgaccg taacgaacgt gtcggtgtta aatttagcga tagtgacttg
     8941 attggtcttc caatccgtat cactgttggt aaaaaagcgg ccgatggcat tgtagaagtt
     9001 aagatcaagg caactggtga caccattgag gttcatgcag ataacgtgct tgaaacgctt
     9061 gaaatcctaa gcaagaaata aaaactataa tcagaagaaa aacaaggaaa aaatgtaact
     9121 agtttttacc ttgttttttc tgtataatgg gagaaatgag tagataaaga ggtaaatgtt
     9181 atgctaagat ttccaaagga ttttgtctgg ggatcctcta cttctggacc gcagacagaa
     9241 ggacgtgtag ctggtgacgg taagggagac aatctctggg attactggtt ccaagtggag
     9301 ccaaatcgtt actataatgg gattggttca gataagacat cgacttttta tgaaaattgg
     9361 gagcgggata ttgagctttt gttagagact ggtcacacag tctttcggac ttctattcag
     9421 tggtcacgga tttttccaca aggctgtgga aaagtcaacc ctcaaggtgt ggatttttat
     9481 cgtaaggtct ttgaggctat taaggctaaa ggaattcgtc tgttagtcaa tctctatcat
     9541 tttgatttac cttttgccct tcaagaggat ggtgatggtt gggaaaataa ggcgacagtc
     9601 tcagcctatg aagactatgc tcgtttttgt tttgagactt atggagattt agtggatcaa
     9661 tggattacct ttaacgagcc catcgttcct gtagaatttg gttattttta tgatgcccat
     9721 tatccacata aggtggatgc agaggcagct gttaaggtag cctatcatac acaattggcc
     9781 agcagccggg ctgttaaggc ttgccatgaa cttttgcctg attccaagat tgggattgtc
     9841 ctcaacttga caccggctta tccacgtagc cagcatcctg ctgatgtcaa ggcagctcgt
     9901 attgcggccc tttttcaggc ccaatctttc ttagatccat ctgtcttggg gacttatcca
     9961 caggagttgg tagaaatctt gcatgaacac ggtcttttac ctgatgctac agaggaagag
    10021 ttggaactca ttcgtgataa tacggtggac ttccttggtg tgaactacta tcaacctttg
    10081 cgtgttatgg ctcctcgatt tgctaagcat ccagatagtc cactcttacc agaacatttt
    10141 tacgagcctt atgtgatgcc tggacgtaaa atcaattctc accgtggttg ggagatttat
    10201 gagcaaggga tttatgacat cgcccaaaat atcaaggaaa attatggcaa tattgagtgg
    10261 atgttgactg agaatggtat gggtgttgaa ggggaagaaa aattccgtca agatggaatg
    10321 attcaagatg attaccgtat tgactttgta aaaggtcatc ttcgtgaact tcaccgtgcc
    10381 attgaagatg gtgccaactg taagggctac ttgatttgga cctttattga ctgctggtca
    10441 tggctcaaca gctataaaaa tcgctatggt ttggtcgaat tagacttgga aacgcaagaa
    10501 cgtcgtctga agaaatcagg ccactggttc aaagaattaa gcgataataa tggattttaa
    10561 aaaggactct gactgattat cccaatcggt cagagttttt tgcttacagg agcttaattt
    10621 gaactatacc aatttttact cttgacaagt gaaagaaaca agtatatact gtttttgctg
    10681 attctataaa agatgaatta gactatacca attttaagga gaaagcacag cttgcctgtg
    10741 tcgtatatac tatgtgtgga attgttggtg ttgttggaaa cacaaatgca actgatattt
    10801 tgattcaagg gcttgaaaag cttgaatacc gtggctatga ttctgcggga atttttgtcc
    10861 tagatggtgc tgataaccat ttggtgaagg cggttggtcg tattgcagaa ttgtctgcca
    10921 agacagctgg tgttgaggga acaactggta tcggacatac tcgttgggct actcacggaa
    10981 aaccaactga ggacaatgct cacccacacc gctctgagac agaacgtttt gtcttggtgc
    11041 ataatggggt gattgagaac tatcttgaaa tcaaggaaga ataccttgca ggtcaccact
    11101 tcaaggggca gacagatact gaaatagccg ttcatttgat tggaaaattt gcggaagaag
    11161 aagggctctc agttcttgaa gccttcaaaa aagcccttca catcatccgt ggttcttatg
    11221 cctttgcctt ggttgactca caagatcctg aagtcatcta cgtagctaaa aataaatcac
    11281 cacttttgat tggtcttgga gaaggctata acatggtctg ctcagacgcc atggctatga
    11341 ttcgtgaaac taaccaatac atggaaatcc atgaccaaga gttggtaatc gtcaaggctg
    11401 atagtgtgga agttcaagac tatgatggaa atcgtcgtga gcgtgctagc tacactgctg
    11461 agcttgactt gtcagatatc ggtaagggaa cttaccctta ctacatgctc aaggaaattg
    11521 acgagcaacc aactgttatg cggaaactca tccaagccta cacagatgag gctggtcaag
    11581 ttgtcgtgga cccagatatc attaaggctg ttcaagacgc agaccgcatc tacatccttg
    11641 cagctggaac atcttaccat gcaggatttg cttctaagaa aatgttggaa gaattgacag
    11701 atacaccagt tgaacttgga atctcatctg agtggggcta cagtatgcca cttctcagca
    11761 agaaaccact cttcatcttt atcagccaat ctggtgaaac agcggatagt cgtcaagttt
    11821 tggtcaaggc taatgaaatg ggaattccaa gcttaacagt gacaaacgtt ccaggttcaa
    11881 ccctctcacg tgaagccaac tataccatgc tccttcacgc aggtcctgaa attgccgtgg
    11941 catcaactaa agcctataca gcgcaaatcg cagcccttgc cttccttgca aaagcagtcg
    12001 gagaagcaaa tggtaatgct aaagcgcaag cctttgacct ggttcatgaa ttgtcaatcg
    12061 tagctcagtc tatcgaatca actctttcag agaaagaaac cattgaagcc aaggttcgtg
    12121 aacttcttga aacaactcgt aacgcctttt acatcggacg tggtcaagat tactacgtag
    12181 ccatggaagc aagtctcaaa ctcaaagaga tttcttatat ccagtgtgaa ggttttgcgg
    12241 caggagaact caagcacgga accattgcct tgattgaaga aggaacgcct gttttggctc
    12301 tcttgtcaga tccagttctt gccaaccata ctcgtggaaa tatccaagag gtcgcagccc
    12361 gtggtgccaa agtcctcact atcgcagaag agaatgtagc caaagatacc gacgatatcg
    12421 tccttacgac cgtacatcca tacctctcac caatttcaat ggtcgtacca acgcaattag
    12481 tcgcttactt tgcaaccctc caccgtggcc tcgatgtgga taaaccacgt aaccttgcta
    12541 aatcagtaac ggtagaataa gctaaaaaag tctagttatc taggcttttt ctaatgagca
    12601 aattggttgc ttgtacttaa gattcgtgtt agaataattt ttcaaattga aggacagaga
    12661 tagacaagga gaatcacaat ggtagaattg ggaatttcaa catttgggga aataacggag
    12721 cttgaaggga ctggacaaac ttacagtcat gctgaacgca ttcgccagtt ggtggcagag
    12781 attgagctgg ctgacaaggt tggtttggat gtatatggga ttggtgagca ccatcgggcg
    12841 gattttgcag tatcagcccc agagattgtt ctggccgctg gggcagtcaa taccaagaaa
    12901 atccgtttga ccagtgcagt cagcattctg tcaagcatgg acccgattcg tttgttccaa
    12961 cagtatgcca ctatcgatgc tttgtcaaat ggacgagcgg agattatggc tggaaggggc
    13021 tctttcacgg aatctttccc tctgtttggt tatgacttga aagactacga ctctcttttt
    13081 gatgagaaat tagacttgct tcagttagtc aatgaaaaga ccaagttaga ctggcaaggt
    13141 cgattgactc aaacgattgc tggtaaagaa gtttatcctc gtccagttca ggacaaattg
    13201 cccttgtgga tagctacagg tggtcatgtc gaatcaacag tgaagattgc tcaggctggt
    13261 ctaccgattg tatatgctat tattggtggt aatccacgtt attttaaaaa gttgattcag
    13321 gcttatcgtg agattggaag cgaagcgggt catgctgaca aagacttgaa agtgggagcc
    13381 cattcttggg ggtggattgc ggaagatggt gagcaggcgg tgaaagatta tttccatccg
    13441 actaagcaag tggtggatgc tatttccaaa gaccgtccgc actggcagga attgcgttat
    13501 gagcaatatt tggagcaggt agggccaaat ggcgccatgt ttgttggcaa tccagatcag
    13561 gtggccgaaa aattgattcg catgatagag gatttaggat tggaccgctt catgcttcat
    13621 ctaccgcttg gttctatgcc tcatgaccag gttctgagag ctattgaact cttcggaaca
    13681 caagttgcac ccaaagtacg agcttatttt gctatgaaag aggcttaata aaaaatccta
    13741 atcaagttta ttaggacaac aaatattgta cgaaaattaa tcccccgagc tataatgctt
    13801 gggggatttt tctataggag ggctagttta gttctctttt ttatttttta gtaagaaacc
    13861 gagaccaagg agcgcaagga ggctgattcc tgcaaatagg agtttgtttt cgtttttgat
    13921 tcctgtattt ggaagttcag cttgtttatc tggggttgca ggtatatttt ccttgctaga
    13981 gttttctacc gattcgtttc gaaccgcttc ttttacagat gatgcttgtg cttcttgtgc
    14041 tggttgttca gcttgtgaat cagctgtagc ttggctaggt ttattttccg catcagctac
    14101 tttggcatct ggtttagtag aatcaggtct agcttctgga gctgggtctt gatgtgcagg
    14161 gtgagaagct tcattttgat gttcaggctt ggaaggggtt gagtctggtt tctcttctgc
    14221 agttgactca tggctagttc cattttgaga gactcgaaga acagtagctg taagggcatt
    14281 caatttcaag cctttttcag tccattcaag tcctttcggg ttggcaattc cgactggtcc
    14341 tgcttggttt tcatctgcca aaacttccgc atttcttaga tgtgcaaagg cagttcccaa
    14401 attaaattcg cgagcttttt catccgcatt gacaaagact gcgtagatat cgccgtttgg
    14461 agcagtgatt tggtagccaa tcactacatc ctctttttcc acaccatttt ggcctgggac
    14521 agtgatgagg tggacacggt ctttgatatc ttggagactc ttaagtcgga aggcatctgt
    14581 agattgacga agggcaatca aacctttcat atagtcacgg ctcttgacat tttcaggata
    14641 agctttacca tctgtagcct tagtccagtc aaacttgttg actgcgtcgc tagaatcgta
    14701 agaatcatgg atgaagtaag gatagtcaaa tgggttgccg tccttatcac gtaacaagtg
    14761 agatttgttt ggaaccttat cctctgctac tggagtcttg taggctgggt cacggaattg
    14821 tttagtacgt ccatattcct gaccggagtg gataaatgga gttccttgag ctgtcaagac
    14881 catgagattt ccaagtcgta aacgacggtg gatttcagca tagttctcag ccttgcttgg
    14941 gtcttttttg atagactggg caatgatgtc aaagagggtc aagttatcat gggctgctat
    15001 gtattggatg acatctccag ggctgtcagc ttcaaagtta gttggttgag caatgagatt
    15061 tttaaagatg gtgttgacat cacgcttgcc acctgtgata aaggcaggtt gaccttcgtt
    15121 tggataacca gatttgaggt tgttacggat gtcatctgaa aagacagcga cagtatcggt
    15181 atgtttcatc caatcttggt cagcagcttt agtaggcatg ttttcatcac cggcataggt
    15241 tctccaacct tcaccaagca tgatgaggtt tggattgagg gcgcgtgcag ccttgtaagc
    15301 ttcttcgata gaagcggcgt catggtctcc catcatatcg aaacggaatc catccacttt
    15361 gtaggtatca actaggtatt tgatagagtc aactaggagc cgtttggtca tatggtggat
    15421 tgttcccaag cgtccaccac caaagctagt tcgaggtgtg ccatcggcat ccataaagtg
    15481 gtagtagttt ggctctaggt cttcaaagat atcgactttg gctgtgtggt tataaacgac
    15541 atctaggata gctcccatac cacgtttgtg gatttcgttg atgaggtttt taaattctgc
    15601 gattcgtttt tctggattct taggatcgct tgagtacata ccagtcaagg agaagtagtt
    15661 ttgagggtca tatccccagt tgtagttgct gttgcttgaa gtgtagtcag acaagcgttc
    15721 atggtttttc aattcattga caaagtagta agacaagact ggaaggagct ggatatgggt
    15781 tacacccaag tctttgagat agtctagttt ttcaatgaag gcttcaaaag tcccaaatgg
    15841 tttggtcaag tcttttgcaa tggcaggatc tgaagtgaag tcacgcacat gagcttcgta
    15901 gataacggcg tcttcacgag tcttgaaatt gtgaatctta ccataagtca agtcttgagg
    15961 tccgagttta gctggatcta caaaggcggc tttagccact ttatgggcat cgtcaatttt
    16021 ggcatcgtcg ctattccaag cagcaagaga tttagcgtaa ggatcgagtg caagaacagt
    16081 tttaccttga cgctcgattt ggtattgata atagtagcca gtgaaatctg tgattccgag
    16141 tttgtttgtg ctgtctagag tttgtttcca agttcctctt tccccttttt cgagagcgac
    16201 agttccaact actttgtcag ggtcattctt gtcgtagaca acaacagaaa ccttatcagc
    16261 acttggtgac caaagggtca aatcaacttg ttttccttct tcttttaggt cagctcccag
    16321 tttgccatca tagctgtatg tctcatcttt caggcgccag cttgttttgg tagtgaattg
    16381 gtcggaattg tagctaacag tataaggatg ttttgtgtca gagaaatctc cgctgtaggt
    16441 cactttctta ccagcttcat cgattgcaac atcggtaata gttaccttgt ttcctaggtg
    16501 attagtgatg ttggagtgtt tgaggatatc ttctttttta gcaccgacaa gtgttgaaaa
    16561 gctactttca atgctagaag tgcctacgtg ttgggctcct gtcatacgga tatcatggac
    16621 atagtatgga tttgtgtaaa tcgattcatc atcgtctttt aggaaaattt ggctatgatt
    16681 tttcaaatct gtgaacttat aattttcttt acggattttc acgtcgtctc cttgtttgct
    16741 ctcatctagt aataaaaatc caaattctct tgcggcttca ttaagaggaa tgtcgatata
    16801 gcggccatat ttgcctgtag ccgtaaagtc tgttccgtca ggccattgag cgctacttgg
    16861 attttttaca tctccccagt accagagaga tttcttgtca tagttgccat ctgtgcggta
    16921 gtagttgacg cgaacagttc ctgcaggctg tggctcgtaa gagaaaacct tgtaatcttg
    16981 gtctaaccaa gcttcgttca tttttggaac tagtttttct acagatttat cgccggttag
    17041 attttttcca gctgtattgt tgatgaggaa gctaattttc ttggcttgtt ctccctttaa
    17101 tttgacatct aggtaatagc cgtagtcatc tttcttggca tccttgaagg acaaagctcc
    17161 gtttggccag ttttcagatg gtttttcaac atcgtcccaa gtccatagtc cttgagcatc
    17221 cttgttttct tcaggaagtt ttttgacatg gatacggaag tagttatctt cgatgggctc
    17281 ttctgcctta gtttcagttg ttactggact agtagaagta gttggctctg ttgcaggttt
    17341 ttcttctgtt ttctctaggg aagcgtttgc atttctaggt gaagaaatat cactttcgtt
    17401 cttaccagag ccagctgctg tatcagtaag aggttgggca agggtggttg tatttctgtt
    17461 gttgggttcg ttagtggttg gtgtactttc gtcggatgag atagttggtg tagccatggc
    17521 aagcaagaca aggcttgcgc cgataaggac agaaccagtt ccatttttga gggaacggat
    17581 gctgtagacc atttttttct cagtgtgaga tggtgttttt ctcataatga ttctccaatc
    17641 aataaatttc tatatcagta tagcatgtag taaaacaata tgcaagcgtt ttctttgatt
    17701 tgtgaaaaaa attcttactt ttgagaagtt ttctgtacta ttttatatat cagtataaat
    17761 gacggagata aaaatgtcac ataagccaag ccgtattatt aggaagatga tgtgttccca
    17821 gctattttta ttcagaaatt gatctctaac attaccaata aaaaagaaaa atatcttgac
    17881 aagcaaggga aaattttgtt acaataatag acggtacttt ttacttttgg tctctcaaga
    17941 gtgtacaggg acgtgctgac aaatgttgca aaagtacaca cagatgatat ctgtcaccaa
    18001 gtgtatcatc accaaaaata aaaaaacaca ggagaatgta gatgcctaca attaaccaat
    18061 tggttcgcaa accgcgtaaa tcaaaagtag aaaaatctaa atcaccagct ttgaacgttg
    18121 gttacaatag tcataaaaaa gttcaaacaa acgtttcttc accacaaaaa cgtggtgttg
    18181 caactcgtgt tggaacaatg acacctaaaa aacctaactc agcccttcgt aaattcgctc
    18241 gtgtacgttt gagcaacctt atcgaagtta ctgcctacat cccaggtatc ggacacaact
    18301 tgcaggagca cagcgtggtg cttcttcgcg gtggacgtgt aaaagacctt ccaggggtac
    18361 gttaccatat cgtccgtggt gcacttgata ctgcaggtgt taacgatcgt aaacaaggcc
    18421 gttctaaata cggtactaaa cgtccaaaag cataaggaaa ggggataaag agaaatgagt
    18481 cgtaaaaata gagctccaaa acgtgacgta ttgccagatc cgctatacaa ttcacaacta
    18541 gttactcgtc ttatcaaccg cgttatgctt gatggtaaac gtggtactgc tgcttcaatc
    18601 gtttacggtg cttttgagca aatcaaagaa gctactggca acgatgcact tgaagtattt
    18661 gaaacagcta tggaaaacat catgcctgta cttgaagtac gtgcacgtcg tgttggtggt
    18721 tctaactacc aagtcccagt tgaagttcgt ccagaacgtc gtacaacact tggacttcgt
    18781 tggttggtaa caatcgctcg tcttcgtggt gaacacacaa tgcaagaccg tcttgcaaaa
    18841 gaaatcttgg atgctgctaa caacactggt gcagcagtta agaaacgtga agatactcac
    18901 cgtatggctg aagctaaccg tgcattcgca cacttccgtt ggtaagatag gatgcgaaag
    18961 cgttaagaaa gtcccagaga aaatagggaa tcgaagcagg ttgcggttgc aaccaatgag
    19021 attcatcttt ttctccagac ttttagcttg agctcaacta aatcatgatg ctaggaacgg
    19081 taaggatgca aggtgaaaat aggaaactga cgcagtattc gacgaataca aggagtttta
    19141 tctttttcac gcagcatccc gttccagctc acatcggcta actaacttta gcccgggttc
    19201 aaattagcta aatcgattag tattagctat aactcagctt accatctcgt aagttgaaac
    19261 caacaatagc atgaaaacat tgagaacggg taggtcctgc ctatccgttt ttattaaaat
    19321 cgtgttataa tagaatagaa atcaaaaata aataggagaa acaaacctca tggcacgcga
    19381 attttcactt gaaaaaactc gtaatatcgg tatcatggct cacgtcgatg ccggtaaaac
    19441 aacaactact gagcgtattc tttactacac tggtaaaatc cacaaaatcg gtgaaactca
    19501 cgaaggtgcg tcacaaatgg actggatgga gcaagagcaa gaacgtggta tcacgatcac
    19561 atctgctgcg acaacagctc aatggaacaa ccaccgcgta aacatcatcg acacaccagg
    19621 acacgtggac ttcacaatcg aagtacaacg ttctcttcgt gtattggatg gtgcggttac
    19681 cgttcttgac tcacaatcag gtgttgagcc tcaaactgaa acagtttggc gtcaagcaac
    19741 tgagtacgga gttccacgta tcgtatttgc caacaaaatg gacaaaatcg gtgctgactt
    19801 cctttactct gtaagcacac ttcacgatcg tcttcaagca aatgcacacc caatccaatt
    19861 gccagtcggt tctgaagatg acttccgtgg tatcattgac ttgatcaaga tgaaagctga
    19921 aatctatact aacgaccttg gtacggatat ccttgaagaa gacatcccag ctgaatacct
    19981 tgaccaagct caagaatacc gtgaaaaatt gattgaagca gttgctgaaa ctgacgaaga
    20041 attgatgatg aaatacctcg aaggtgaaga aatcactaac gaagaattga aagctggtat
    20101 ccgtaaagcg actatcaacg ttgaattctt cccagtattg tgtggttcag ccttcaaaaa
    20161 caaaggtgtt caattgatgc ttgatgcggt tatcgactac cttccaagcc cacttgacat
    20221 cccagcaatc aaaggtatta acccagatac agacgctgaa gaaattcgtc cagcatctga
    20281 cgaagagcca tttgcagctc ttgccttcaa gatcatgact gacccattcg taggtcgttt
    20341 gacattcttc cgtgtttact caggtgttct tcaatcaggt tcatacgtat tgaatacttc
    20401 taaaggtaaa cgtgaacgta tcggacgtat ccttcaaatg cacgctaaca gccgtcaaga
    20461 aatcgacact gtttactcag gtgatatcgc tgctgccgtt ggtttgaaag atactacaac
    20521 tggtgactca ttgacagatg aaaaatctaa aatcatcctt gagtcaatca acgttccaga
    20581 accagttatc caattgatgg ttgagccaaa atctaaagct gaccaagaca agatgggtat
    20641 cgcccttcaa aaattggctg aagaagatcc aacattccgc gttgaaacaa acgttgaaac
    20701 tggtgaaaca gttatctcag gtatgggtga acttcacctt gacgtccttg ttgatcgtat
    20761 gcgtcgtgag ttcaaagttg aagcgaacgt aggtgcgcct caagtatctt accgtgaaac
    20821 attccgcgct tctactcaag cacgtggatt cttcaaacgt cagtctggtg gtaaaggtca
    20881 attcggtgat gtgtggattg aatttactcc aaacgaagaa ggtaaaggat tcgaattcga
    20941 aaacgcaatc gtcggtggtg tggttcctcg tgaatttatc ccagcggttg aaaaaggttt
    21001 ggtagaatct atggctaacg gtgttcttgc aggttaccca atggttgacg ttaaagctaa
    21061 gctttatgat ggttcatatc acgatgtcga ctcatctgaa actgccttca agattgcggc
    21121 ttcactttcc cttaaagaag ctgctaaatc agcacaacca gctatccttg aaccaatgat
    21181 gcttgtaaca atcactgttc cagaagaaaa ccttggtgat gttatgggtc acgtaactgc
    21241 tcgtcgtgga cgtgtagatg gtatggaagc acacggtaac agccaaatcg ttcgtgctta
    21301 cgttccactt gctgaaatgt tcggttacgc aacagttctt cgttctgcat ctcaaggacg
    21361 tggtacattc atgatggtat ttgaccacta cgaagatgta cctaagtcag tacaagaaga
    21421 aattattaag aaaaataaag gtgaagacta atccgtcctc actctagaag gaagtcactt
    21481 agtggcttcc ttttgtcttt agaaaatacc tctaaatatg gtaaaatagt agaagaataa
    21541 tgtgaggaaa atgaatgtca aatagttttg aaattttgat gaatcaattg gggatgcctg
    21601 ctgaaatgag acaggctcct gctttagcac aggccaatat tgagcgagtt gtggttcata
    21661 aaattagtaa ggtatgggag tttcatttcg tattttctaa tattttaccg attgaaatct
    21721 ttttagaatt aaagaaaggt ttgagcgaag aattttctaa gacaggcaat aaagctgttt
    21781 ttgaaattaa ggctcggtct caagaatttt caaatcagct cttgcagtcc tactataggg
    21841 aggctttctc tgaaggtcca tgtgctagtc aaggttttaa gtccctttat caaaatttgc
    21901 aagttcgtgc tgagggtaat cagctattta ttgaaggatc tgaagcgatt gataaggaac
    21961 attttaagaa gaatcatctt cctaatttag ccaaacaact tgaaaagttt ggttttccaa
    22021 cttttaactg tcaagtcgag aagaatgatg tcctgaccca agagcaggaa gaggcctttc
    22081 atgctgaaaa tgagcagatt gttcaagctg ccaatgagga agcgctccgt gctatggaac
    22141 aactggagca gatggcacct cctccagcgg aagagaaacc agtctttgat tttcaagcga
    22201 aaaaagctgc agctaaaccc aagctggata aggcggagat tactcctatg atcgaagtga
    22261 cgacagagga aaatcgtctg gtatttgaag gggttgtttt tgatgtggaa caaaaagtga
    22321 ctagaacagg tcgtgtttta atcaacttta aaatgacgga ctatacttca agtttttcta
    22381 tgcaaaagtg ggttaaaaac gaggaagagg cccagaagtt tgacctcatc aagaagaatt
    22441 cttggctccg agttcgaggg aatgtggaga tgaataactt cacacgcgat ttgattatga
    22501 acgtacagga tctgcaggaa gttgttcact atgagcggaa ggatttgatg ccagaaggtg
    22561 agcgtcgggt tgagtttcat gctcatacta acatgtcgac tatggatgct ttgccagagg
    22621 tcgaagagat tgttgcaaca gctgctaagt ggggacacaa ggcggttgct atcacagacc
    22681 atgggaatgt ccagtccttt ccacatggct ataaggcggc taagaaagcg ggaatccagc
    22741 tgatctatgg gatggaagcc aatatcgtgg aggaccgtgt ccctatcgtc tataacgaag
    22801 tggagatgga cttatcagaa gcaacctacg tggtctttga cgtggaaacg acgggacttt
    22861 cagctatcta taatgacttg attcaggttg cggcctctaa gatgtacaag gggaatgtta
    22921 ttgctgaatt tgatgaattt atcaatcctg ggcatccctt gtcagctttt actacagagt
    22981 taactggaat tacagatgat catgtcaaaa atgccaaacc actagaacaa gttttgcaag
    23041 aattccaaga attttgcaag gatacggtcc tagttgccca caatgctacc tttgacgttg
    23101 gctttatgaa tgctaattat gagcgtcatg gtcttccaaa gattagtcag ccagttattg
    23161 atacgctgga gtttgctaga aacctctatc ctgagtataa acgccatggt ttggggcctt
    23221 tgaccaagcg ttttggtgtg gccttggaac atcatcacat ggccaactac gatgcggaag
    23281 cgactggtcg tctgcttttc atctttatca aagaggtagc agaaaaacat ggtgtgaccg
    23341 atttagctag actcaacatt gatctaatca gtccagattc ttacaaaaaa gctcggatca
    23401 agcatgcgac catctatgtc aagaatcagg taggtctaaa aaatatcttt aagctggttt
    23461 ccttgtctaa taccaagtat tttgaaggag tgccacggat tccgagaacg gttctagatg
    23521 cccatcgaga gggcttgatt ttaggttcag cctgttcaga gggtgaagtt tttgacgtgg
    23581 tcgtttctca aggtgtggat gcggcggttg aggtggccaa gtattatgac tttatcgagg
    23641 tcatgccacc ggctatctat gcgcccttga ttgctaaaga gcaggtcaag gatatggagg
    23701 aactccagac cattatcaag agtttgatag aggttggaga ccgccttggc aagcctgttt
    23761 tggctacggg aaatgttcac tatatcgaac cggaagaaga gatttatcgt gaaattatcg
    23821 tccgtagttt gggacagggt gcgatgatta accgaactat cggtcatggt gaacatgccc
    23881 aaccagcacc acttccaaag gctcattttc gaacgactaa tgagatgttg gatgaatttg
    23941 cctttttggg agaggaactg gctcgtaaac tggttattga aaacaccaat gccttggcag
    24001 aaatatttga acccgttgaa gtcgttaagg gtgacttgta tacgcctttc atcgacaagg
    24061 ctgaagaaac agttgctgag ttgacctata agaaagcttt tgagatttat ggaaatccgc
    24121 tgccagatat tgttgatttg cggattgaaa aagaattaac atccatactg gggaatggat
    24181 ttgctgtgat ttatctggca tcgcagatgc tggtgcaacg ttctaatgaa cggggttatt
    24241 tggttggttc tcgtgggtct gtcggatcta gtttcgttgc gaccatgatt ggaattacgg
    24301 aggtcaatcc tctctctcct cactatgtct gtggtcagtg tcagtacagt gagtttatca
    24361 cagatggttc gtacggttca ggatttgata tgccccataa ggactgtcca aactgtggtc
    24421 acaaactcag taaaaacgga caggatattc cgtttgagac cttccttggt tttgatgggg
    24481 ataaggttcc tgatattgac ttgaacttct cgggagaaga tcagcctagc gcccacttgg
    24541 atgtgcgtga tatctttggt gaagaatatg ccttccgtgc gggaacagtt ggtacggtag
    24601 ctgccaagac tgcctatgga tttgtcaagg gttacgagcg agattatggc aagttttatc
    24661 gtgatgcaga agtagaacgc ctcgctcaag gagcggcggg tgtcaagcgg acaacaggcc
    24721 aacacccggg gggaatcgtt gttattccga actacatgga tgtctacgat tttacgcctg
    24781 tccagtatcc agcagatgat gtcacggctg aatggcagac cactcacttt aacttccacg
    24841 atatcgatga gaacgtcctc aaactcgatg tactgggaca tgatgatccg accatgattc
    24901 gaaaacttca ggatttgtct ggtattgacc ctaataaaat tcctatggat gacgaaggcg
    24961 tgatggcact cttttctggg actgatgtgc taggggtaac acctgaacaa attggaacgc
    25021 ctacgggtat gttggggatt ccagagtttg gaacaaattt cgtacgtgga atggtagacg
    25081 aaacccatcc gacaaccttt gcggaattgc ttcagctgtc tggtctgtcc cacggtactg
    25141 atgtttggtt ggggaatgct caggatctga ttaagcaagg aatagcggac ctatcgactg
    25201 ttatcggttg tcgggacgac atcatggttt acctcatgca tgcgggtctg gaacctaaga
    25261 tggcctttac cattatggaa cgggtacgta agggtttgtg gctaaagatt tcagaagagg
    25321 agagaaatgg ctatatcgaa gcaatgaagg ctaataaggt gccagagtgg tatatcgaat
    25381 cctgtgggaa aattaagtac atgttcccta aggcccatgc ggcagcctac gttatgatgg
    25441 ccttgcgtgt agcttacttc aaggttcacc atcctattta ttactactgt gcttacttct
    25501 ccattcgtgc taaggctttt gatatcaaga ccatgggtgc gggcttggag gtcatcaagc
    25561 gcagaatgga agaaatctct gaaaaacgga agaacaatga agcctctaat gtggaaatcg
    25621 atctctatac aactcttgag attgtcaatg agatgtggga acgaggtttc aagtttggta
    25681 aattagatct ctaccgtagt caggcgacag agttcctcat cgacggggat acccttatcc
    25741 caccatttgt agcaatggat ggtctgggag agaacgttgc caagcaactg gtgcgggcgc
    25801 gtgaagaggg agaattcctc tctaaaacag aactacgcaa gcgtggtgga ctctcatcaa
    25861 ccttggttga aaagatggat gagatgggta ttcttggaaa tatgccagag gataaccagt
    25921 tgagtttgtt tgatgagttg ttttaaaaaa ttgcttaata atctattaaa agaggctaac
    25981 gtatatccaa tagatttaca ttagctttct tttttgttaa aatagtctat ggaaagaggg
    26041 tgagagtatg tcaaagatga gtataagcat ccgtctggat agtgaggtta aggagcaggc
    26101 ccaacaggtg tttagtaatc tgggaatgga tatgacaaca gctattaata ttttccttcg
    26161 tcaggcaatt caatatcagg gattaccttt tgatgttaga ctagacgaaa atcggaagtt
    26221 gctccaagcg ttaacggatt tagaccaaaa tcgtaatatg agccagtctt ttgaatcagt
    26281 ctcagatttg atggaggact tacgtgctta agattcgtta tcataaacag tttaaaaaag
    26341 attttaagtt ggctatgaag cgtggtttga aggcagaatt attagaagaa gttttgaatt
    26401 ttctggttca agaaaaagaa catcctgcca gatatcgtga tcattcattg acggcatcca
    26461 agcattttca aggagttcgt gaatgccata cccagccaga ttggcttttg gtttataaag
    26521 tagacaagtc ggaattgatt ttaaatttgc tgaggacagg cagtcacagt gatttatttt
    26581 aatctatttt aagggggttc tcatgaaact aagaatattt gcggaagata agccggctaa
    26641 gaaggtattt gaatatcaat tagaacttgc tgatcgtaca attcttctat cgacagcact
    26701 cttgtcaggt gctattgctt tagcaggaat cttttctgct ttgaaagaaa aataaaaata
    26761 gaaaagagaa aacagaatgg ttttaccaaa ttttaaagaa aatctagaaa aatatgcgaa
    26821 attgttggtt gcgaacggaa ttaacgtgca acctggtcac actttggctc tctctattga
    26881 tgtggagcaa cgtgaattgg cacatctaat cgtgaaagaa gcttatgcct tgggtgcgca
    26941 tgaggtcatc gttcagtgga cagatgatgt gattaaccgt gagaaattcc tccatgcccc
    27001 gatggagcgt ttggacaatg tgccagaata caagattgct gagatgaact atcttttgga
    27061 gaacaaggct agccgtcttg gggttcgttc atctgatcca ggtgccttga acggagtgga
    27121 cgctgacaag ctttcagctt ctgctaaagc tatgggactt gccatgaagc ctatgcgtat
    27181 cgcaactcaa tctaacaagg ttagctggac tgtagcagcc gctgctggac ttgagtgggc
    27241 taagaaagtc ttcccaaatg ctgcgagcga cgaagaagca gttgatttcc tttgggatca
    27301 aatcttcaaa acttgtcgtg tctacgaagc agatcctgtt aaggcttggg aggaacatgc
    27361 agccattctc aagagcaagg ccgatatgct taataaggag caattttcag cccttcacta
    27421 cacagcgcca ggaacagatt taacacttgg tttgccaaag aaccacgttt gggaatcagc
    27481 tggtgctgtc aatgcacagg gcgaagaatt cttgccaaat atgccgacag aagaggtctt
    27541 cacagcgcct gacttccgtc gtgcagatgg ttatgtcact tctacaaaac cgcttagcta
    27601 caacggaaat atcattgaag gcattaaggt gacctttaag gatggacaaa tcgtagatat
    27661 cactgctgag aagggtgatc aggttatgaa agaccttgtc tttgaaaatg cgggtgcgcg
    27721 tgccttgggt gaatgtgcct tggtaccaga tccaagtcca atttctcagt caggcattac
    27781 cttttttaac acccttttcg atgaaaatgc gtcaaaccac ttggctatcg gtgcagccta
    27841 tgcgactagc gttgttgatg gagcggagat gagcgaagag gagcttgaag ctgcagggct
    27901 taaccgttca gatgttcacg tagactttat gattggttct aaccaaatgg atatcgatgg
    27961 tattcgtgag gatggaacgc gggtacctct tttccgtaat gggaattggg caaattaagg
    28021 agataatatg ttaggaagta tgttcgttgg tctcctagtg ggatttttag caggtgctat
    28081 gaccaatcgt ggagagcgaa tgggatgttt tggaaaaatg tttctcggtt ggatcggagc
    28141 ctttctaggt cacttgctct ttggaacttg ggggccagtt ttatcaggaa cagctattat
    28201 cccagcgatt ttaggagcca tgattgtttt agctattttt tggagacgag gaagttaatt
    28261 tcctaaatct gatactcaat gaaaatcaaa gagcaaacta ggaaactagc cgcaggttgc
    28321 tcaaagcact gctttgaggt tgcagataga actgacgaag tcagctaaaa acactgtttt
    28381 gaggttgcag atagaactga cgaagtcagt aacatatata cggcaaggcg acgttgacgc
    28441 ggtttgaaga gatttttgaa gagtatgaaa cgaaaaggag gttggtcatt gtactagcct
    28501 ccttttgata tgatataata gttctatgag attagataaa tttttagttg cctgtgcggt
    28561 gggaagtcgg actgaggtca aaaacttgct caaggctggg cgcgtgactg taaatggtaa
    28621 aaaagaaaaa tcagctaaat tgcagattga tgaaaaaata gatgagattc gctttgatgg
    28681 gcaagtgttg gagtatgaag agtttgtcta ctacatgatg aacaagccca aaggagttat
    28741 ctcagcgact gaggatccca agcacagaac cgttctggac ttgttggatg acttggcgcg
    28801 gagtaaggaa gtttttccag taggacgctt ggatattgac acgcatggtc ttttgctctt
    28861 gaccaataat ggtcagctgg ctcatgttct tctttcgccc aagcgtcatg tggacaagac
    28921 ttatcaggct caggtcaagg gaattatgac ccaagaagat gtggagacat ttatcaaggg
    28981 cattccgctc aaagacttta cctgtcagcc cgctagactg gagattcttt ccacagatgc
    29041 agaaaagaat cagagccaaa tccgtgtgac cattgcagaa gggaagtttc atcagatcaa
    29101 gcgtatggtg ggctactgtg gcaaggaagt ggtggacttg caacgattga ctatgggaac
    29161 tttagtatta gatgagaacc tggaacgagg ggaatggcgt cgcttgacca aggaagaatt
    29221 agaaattctc cgcgctaata ttatttaatt tagt
//
LOCUS       DAVKLX010000027        28760 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKLX010000027 DAVKLX010000000
VERSION     DAVKLX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.027"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            537..1700
                     /locus_tag="VW325_001232"
     CDS             537..1700
                     /locus_tag="VW325_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9734641.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDREVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVTFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1697..2374
                     /locus_tag="VW325_001233"
     CDS             1697..2374
                     /locus_tag="VW325_001233"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9734642.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2364..3218
                     /locus_tag="VW325_001234"
     CDS             2364..3218
                     /locus_tag="VW325_001234"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9734643.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3237..4304
                     /locus_tag="VW325_001235"
     CDS             3237..4304
                     /locus_tag="VW325_001235"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9734644.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            4314..5480
                     /gene="aroC"
                     /locus_tag="VW325_001236"
     CDS             4314..5480
                     /gene="aroC"
                     /locus_tag="VW325_001236"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9734645.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5490..6593
                     /locus_tag="VW325_001237"
     CDS             5490..6593
                     /locus_tag="VW325_001237"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9734646.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6604..6942
                     /locus_tag="VW325_001238"
     CDS             6604..6942
                     /locus_tag="VW325_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9734647.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            7034..8317
                     /gene="aroA"
                     /locus_tag="VW325_001239"
     CDS             7034..8317
                     /gene="aroA"
                     /locus_tag="VW325_001239"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9734648.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8310..8786
                     /locus_tag="VW325_001240"
     CDS             8310..8786
                     /locus_tag="VW325_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9734649.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKREAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIVADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8783..9631
                     /gene="pheA"
                     /locus_tag="VW325_001241"
     CDS             8783..9631
                     /gene="pheA"
                     /locus_tag="VW325_001241"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9734650.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9628..10902
                     /gene="psr"
                     /locus_tag="VW325_001242"
     CDS             9628..10902
                     /gene="psr"
                     /locus_tag="VW325_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9734651.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFVYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMIFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11181..12026
                     /locus_tag="VW325_001243"
     CDS             11181..12026
                     /locus_tag="VW325_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9734652.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSYKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKDPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            12016..13119
                     /locus_tag="VW325_001244"
     CDS             12016..13119
                     /locus_tag="VW325_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9734653.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            13121..14107
                     /locus_tag="VW325_001245"
     CDS             13121..14107
                     /locus_tag="VW325_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9734654.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATDLSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            14123..15556
                     /locus_tag="VW325_001246"
     CDS             14123..15556
                     /locus_tag="VW325_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9734655.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMI
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15585..16619
                     /locus_tag="VW325_001247"
     CDS             15585..16619
                     /locus_tag="VW325_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9734656.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16819..17556
                     /gene="mecA"
                     /locus_tag="VW325_001248"
     CDS             16819..17556
                     /gene="mecA"
                     /locus_tag="VW325_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9734657.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17707..18993
                     /locus_tag="VW325_001249"
     CDS             17707..18993
                     /locus_tag="VW325_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9734658.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRSLEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18995..19864
                     /locus_tag="VW325_001250"
     CDS             18995..19864
                     /locus_tag="VW325_001250"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9734659.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19940..20011
                     /locus_tag="VW325_001251"
                     /pseudo
     CDS             19940..20011
                     /locus_tag="VW325_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            20159..21097
                     /gene="msrB"
                     /locus_tag="VW325_001252"
     CDS             20159..21097
                     /gene="msrB"
                     /locus_tag="VW325_001252"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9734660.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            21155..22879
                     /locus_tag="VW325_001253"
     CDS             21155..22879
                     /locus_tag="VW325_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734661.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQVTSDRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQKLWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDAISALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22881..24629
                     /locus_tag="VW325_001254"
     CDS             22881..24629
                     /locus_tag="VW325_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734662.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANSLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNLEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24976..26235
                     /locus_tag="VW325_001255"
     CDS             24976..26235
                     /locus_tag="VW325_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9734663.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     KAVKEALTEMLQSGTTTFNDMYNPIGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26311..26446
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26485..26985
                     /locus_tag="VW325_001256"
     CDS             26485..26985
                     /locus_tag="VW325_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9734664.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            27061..27429
                     /gene="rplL"
                     /locus_tag="VW325_001257"
     CDS             27061..27429
                     /gene="rplL"
                     /locus_tag="VW325_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9734665.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27676..>28760
                     /locus_tag="VW325_001258"
     CDS             27676..>28760
                     /locus_tag="VW325_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9734666.1"
                     /translation="MSLRNKIEQHIKELEGGKFQKLGDAYLSRKYNFNIVSLGSQEGT
                     DKTTKGIPDSYAVENGKYVYIMYGTHKSVISKLEGDIQSVKKKILEENIAEDKVGRLI
                     CCHTSSNITIKQKEDLEKMAEPYHLELIGINEIANDLTKIDFQYLAKEYLSISESTEQ
                     VWSINDFIRIHDESKTNAPISNDYIGDVSEIINTIKSSEKRIFLISAKPGTGKTRLAI
                     EICSLLDRNKYNIICVKSNNQDIYQDVKRNLNLHKENIVFIDDVNTTQNYISTLGLLN
                     TTSNIRFILTVRDYAKKDVINNIKVYGYNNIESELIKDDNFKELLNQFSRNDFTNQEI
                     EHIKTISKSNPRIAVIAAKLSSSQDLT"
BASE COUNT         8628 a         5274 c         6407 g         8451 t
ORIGIN      
        1 ttatagttta tttattggga tatttttcaa tttcaaaaac gttaattcct ataatgtgtg
       61 tgatccaagt atttttgata gaacatatat ttagagttcg aaatagaatg atgcaaatag
      121 gtgaaatttt aattattgtt gcttctataa tattatttat tgatagtata ttgagtttgt
      181 aacaaaaggc ttttctataa aatcaaattt tccagatatc tactgaagct gatttatttc
      241 ttcatctttc ttctactacc tttgtcacta aatttgacag acacatattg ctgatacaac
      301 ctctctacct ataatctcac ggttaacctg aggctagttt catagtttgc tatttgattt
      361 tcgttgataa aaagaaatgc tcctccaaga cggaagagca ttttttattg tgattcttac
      421 acatttattt cctagttttg caggaagtct agtataccat tttttaaaag aagagtaaaa
      481 aagcaagggt agtttctttt ttgagaattg tctaaaagtt tgatataatg gtagttatga
      541 atagaataag agtcagcaaa agggttgaaa agaagcttgc taaggggcta gttttactag
      601 aagccagtga tcttgagaat gtcaatctta aggatcggga agtagaggtg cagggtcagg
      661 aaggaaactt tcttgggact gcctaccttt ctcagcaaaa caagggcttg ggctggttta
      721 tcagcaaaga caaggtgacc ttcaatcaag ctttctttga aacgttgttt agaaaagcca
      781 aagaaaagag aaacgcctac tatcaagatg atttgacaac tgcctttcgt ctctttaatc
      841 aagagggaga tggctttggg ggtctgacag tggaccttta tggcgactac gccgtctttt
      901 cttggtataa ctcttatgtt tatcagattc gtcagactat atcagaagcc tttagacagg
      961 ttttccctga ggttttaggg gcttatgaga aaatccgctt taagggtttt gactatgaat
     1021 ctgcccatgt ttatggtcaa gaagcacctg actttttcac tgttttagaa aatggtgtcc
     1081 tgtatcaagt ctttatgaat gatggcttga tgacagggat tttcctagac cagcatgagg
     1141 ttcgcggtag tttagttgac ggcttggcta tgggtaaatc cttgctcaat atgttttcct
     1201 acacagcggc tttttcagta gctgcggcca tgggaggagc tagccagaca acttctgttg
     1261 atctagccaa acgttcacga gaattgtctc aagcgcattt tcaggcaaat gggctcagca
     1321 cagacgagca tcgttttata gtcatggatg tctttgagta tttcaaatat gccaaacgca
     1381 aagacttgac ctacgatgtg attgtcctag atccgcctag ctttgctcgg aataaaaaac
     1441 aaactttctc tgtggccaag gattatcaca agttgatttc ccagagtctt gagattttaa
     1501 atccgggagg gattatcatt gccagtacca atgctgccaa tgtttcccgt cagaaattta
     1561 cagaacaaat tgataaaggt tttgcaggaa gaagttacca gattttaaac aaatatggtc
     1621 ttccagcaga ttttgcctat aataaaaaag atgaaagtag taattacctc aaggtgatta
     1681 gtatgaaggt tagtaaatga aattaatcgt ttcagtaatg ccaagaagtt tagaggaggc
     1741 tcaggctctg gatgccacga ggtacctgga tgccgacatc attgaatggc gtgccgacta
     1801 tctgcctaaa gaagcgattt tgcaggtagc tccagccatt tttgaaaaat tcgcaggccg
     1861 tgagttggtt ttcacgctac gaactcgctc cgaaggtgga gaaatcgacc tttctccaga
     1921 agagtatatc catctaatca aggaagttgc gcaactctat caaccagact atattgattt
     1981 tgagtactat agctacaagg atgtttttga ggaaatgctg gacttcccaa atcttgtttt
     2041 gagttaccat aatttccaag aaacacctga gaacatgatg gaaatcttgt cagagttgac
     2101 gatcctaaat ccaaaacttg ttaaggttgc ggtgatggct cacacggagc aggatgtctt
     2161 agacttgatg aactatacac gaggctttaa aaccctcaat cctgaacagg aatatgtgac
     2221 catttctatg ggtaaggtgg gcaaggtctc tcgtatcact gcggatgtga ctggttcgag
     2281 ttggtctttt gccagtctgg atgaggttag tgcccctggg cagatttctc tagctagcat
     2341 gaaaaaaatc agggagattt tggatgaagc ttgatggcta tacacgttta gctgcagttg
     2401 ttgccaatcc tattaagcat tctatttctc ccttcatcca caatagagcc tttgaggcga
     2461 cagctaccaa cggtgcttat gtggcttggg agattgaagc gagtgacttg gcagaaacag
     2521 tggccaatat tcgtcgctac cagatgtttg gcatcaatct gtccatgccc tataaggagc
     2581 aggtgattcc ttatttggat gagctaagcg atgaagcgcg cttgattggt gcggttaata
     2641 cggttgtcaa tgagaatggc aatttaattg gatataatac agatggcaag ggatttttta
     2701 agtgcttgcc ttcttttaca atttcaggta aaaagatgac cctgctgggt gcaggtggtg
     2761 cggctaaatc aatcttggca caggctattt tggatggcgt cagtcagatt tcggtctttg
     2821 ttcgttccgt ttctatggaa aaaacaagac cttacctaga caagttacag gagcagacag
     2881 gctttaaagt ggatttgtgt gctttagaat atgtttctga actgcaagca aggattgccg
     2941 agtcggattt gctagttaat gccaccagtg tgggcatgga tggccaatcc tctcctgttc
     3001 ctgaaaacat agtcttacca gaaactctct tagttgcaga tatcatatac caaccctttg
     3061 aaacaccgtt tttgaaatgg gctagaagtc agggcaatcc agcagtcaat ggtctgggaa
     3121 tgttgctcta tcaagctgca gaagcttttc aactgtggac aggcaaggaa atgccgacag
     3181 aagagatttg gcagtcttta acagaaaaat accaataaag aaagggagac tctcctatga
     3241 aaatcagaat cgatattcct catcatcctt atgatattca gattgaaaaa ggttgtatgg
     3301 cccaggctgg tcagtggttg cgagaactct ggcaaccgca aaaggtagtc attgtgacag
     3361 ataaccatgt agcctctctc tatgcagaga aggtcaagct cagcctagaa gatgctggtt
     3421 ttcaggtagc tgtttttgat ttcttagaag gtgaagaaag aaagaattta actactgttc
     3481 agaaagtcta tgaatttcta gtcaagcaag gtctgactcg tagcgatgga atcgttgctc
     3541 ttggtggtgg cgttgttggg gacctggctg gctttgtagc ctctacctat atgcggggta
     3601 ttcactttgt tcagattccg actagtttga cagctcaggt tgattcttct atcggtggaa
     3661 agacaggcgt taacacacca tttgctaaaa atatggtggg gacctttgcc caaccagatg
     3721 gggttctgat tgatccactt gttcttgaaa ccctcggaaa aagagagttg attgaaggga
     3781 tgggtgaagt tatcaagtat ggcttgattg aggatccaga actgtgggct ctcttgacgg
     3841 gactgaatgg ttctgttgag agtattttgg aacatgcaga gaccttgatt gaacattctt
     3901 gtcaggtgaa gcgcaagatg gtggttgaag atgagttgga caatggtatt cgtctttacc
     3961 tcaattttgg ccacactatt ggccatgcca tcgaagcgac tgccggttat ggcaaggtca
     4021 tgcatggaga ggctgttgcc atgggaatgg tacagatttc caaggttgct gaggaaaaag
     4081 gcctcatgcc agctggcatt acccaatcta tcacagagat gtgtcagaaa ttcggcttgc
     4141 ctgttgacta tgaaaattgg gaagttgaca agctttatca ggctcttact catgacaaga
     4201 aagcgcgtgg taacaccttg aaattggtct tggtgccaga gcttggttca gcgatcattc
     4261 acccagtttc tctggaagag atgaaagact acttggtaaa ataaggagag tgtatgagat
     4321 atttaactgc aggagaatca cacggccccc gtctaacagc tattattgag ggaattccag
     4381 ctggacttcc attgacagct gaggatatca atgaggacct tagacgccgt cagggtggct
     4441 acggtcgtgg tggtcgtatg aagattgaga gtgaccaggt tgtctttact tcgggcgttc
     4501 gccacgggaa gacgacaggg gcgcctatta ctatggatgt catcaataag gaccaccaga
     4561 aatggctgga catcatgtct gcggaggaca ttgaagaccg ccttaaaagc aagcggaaaa
     4621 ttactcatcc tcgcccaggt catgccgatt tggttggggg gattaagtac cgttttgatg
     4681 atttgcgaaa ttctttggag cgttcatcag ctcgtgaaac caccatgcgg gtggcagttg
     4741 gtgcagtagc caaacgcctc ttggctgagc tggatatgga gattgccaac catgtcgtgg
     4801 tctttggtgg caaggaaatc gatgttcctg aaaatctgac agtcgctgaa attaagcaaa
     4861 gagctgccca gtctgaagtt tctattgtca accaagaacg agaacaggaa atcaaggact
     4921 atattgacca aatcaaacgt gatggtgata ccatcggtgg ggttgtggag acagtcgtcg
     4981 gaggcgttcc agttggtctt ggttcctatg tccaatggga tagaaaattg gatgcaagat
     5041 tggctcaagc tgttgtctct atcaatgcct ttaaaggggt ggaatttggt cttggctttg
     5101 aggctggtta tcgtaaaggc agccaagtta tggatgaaat tctctggtct aaagaagacg
     5161 gttatactcg ccgtaccaat aatctaggtg gttttgaagg tggtatgact aatgggcaac
     5221 ccatcgttgt tcgtggggtc atgaaaccca ttcctactct ttataaacct cttatgagtg
     5281 tggatatcga aacccacgaa ccttacaagg caaccgtgga gagaagtgat ccgactgctc
     5341 ttccagctgc aggaatggtc atggaagcag ttgtagcaac ggttctggcg caagaaatcc
     5401 tcgaaaaatt ctcatcagat aatcttgagg aactaaaaga agcggtagcc aaacaccgag
     5461 actatacaaa gaactattaa ggagttccta tggcaaaaac aatctatatc gcaggtcttg
     5521 ggttgattgg agcctctatg gcacttggta tcaaacgcga tcatccagat tatgaaattt
     5581 taggttataa tcgtagtcaa gcttcgagag atatcgcctt gaaagaaggc atgattgacc
     5641 gtgcaacgga tgattttgct agttttgctc ctttggcaga tgtcattatc ctcagcttgc
     5701 caatcaaaca aactattgct ttcattaagg agttggccaa tttggatttg cgagaaggcg
     5761 ttattatttc agatgctggt tcgaccaagt caaccattgt ggatgcggcg gagcagtatt
     5821 tggctggcaa gtctgttcgc tttgtcgggg cccatcccat ggctggtagt cacaagacag
     5881 gggctgcttc ggcagatgtc aatctttttg aaaatgccta ttatatcttt acaccttcaa
     5941 gcctgacaag tcaggacacg cttaaggaaa tgaaggatct gctttcaggt cttcatgctc
     6001 gttttatcga gattgatgcc aaggagcatg atcgtgtcac ttctcagatt agccattttc
     6061 ctcatatttt ggcttctagt ctcatggagc agactgcggt ctatgctcaa gagcatgaga
     6121 tggcaaggcg ctttgcggca ggtggttttc gagatatgac ccgaattgcg gaaagcgagc
     6181 caggaatgtg gacctccatt ctcttgtcca atagcgagac cattctggat agaattgagg
     6241 atttcaagga acgtttggaa gtgattggtc aggccattag taagggagat gaagagcaaa
     6301 tttggaactt ttttaaccaa gcgcgtgaac aacgccagac catggaaatc cataagcgtg
     6361 gtggtgtgga tagctcttac gacctctatg ttgacgttcc tgatgaagaa gatgtcatcc
     6421 tgcggatttt ggaactgcta cgtggaactt ccttggttaa catccacatc aacgaggaaa
     6481 atcgtgagga tattcacgga atcctacaaa tttcatttaa aaatgctcaa gacttggaaa
     6541 gagctgagca tctcataaca gaaaataccg actatacagt cgtcatcaaa taaggagaag
     6601 aacatgtcaa atatttacga tagtgcaaac gaactcagtc gcggtctacg cggattacca
     6661 gaatacaagg ctgttaaagc agctaaagat gcgattgcag cagatgctga ggcaagcaaa
     6721 atctttacag aatatcttgc ctttcaagag gaaattcaaa aactagcaca gacaggtcaa
     6781 atgccagatg cgtccttcca agctaagatg gaaggctttg gtaaacagat tcaaggaaat
     6841 agcctcttgt cagaattctt taccaagcaa caacaattgg ccatttacct ttctgacatt
     6901 gaaaaaattg tttttgaacc agtttcagaa ttgctgaaat aagaaaagaa cgatcctaaa
     6961 gtcaggaatt tttaaggaat ttctggcttt ttatgataaa ataggtaaaa gtattgcaag
     7021 tatgaggtcc agtatgaaac taaaaacaaa cattcgccat ttacatggta gtatccgcgt
     7081 cccaggtgac aagtctatca gccaccgttc cattatcttt ggaagtttgg ctgagggtga
     7141 gaccaaggtt tatgatattc tgcgaggtga agacgttctt tcgaccatgc aggtttttcg
     7201 tgaccttggt gttgaaattg aggataaaga tggggttatt accattcaag gtgtaggcat
     7261 ggctggctta aaagcgccac aaaatgccct taatatggga aattctggca cctcgattcg
     7321 cctgatttca ggtgtccttg ctggtgcaga tttcgaagta gagatgtttg gagatgatag
     7381 tctttccaaa cgtcctatgg accgtgtgac ccttccactg aaaaaaatgg gcgtcagcat
     7441 ttcagggcaa actgaacgag acttgcctcc ccttcgctta aaagggacga aaaacctaag
     7501 acctattcat tatgagttgc caattgcctc tgcccaagtc aagtcagcct tgatgtttgc
     7561 agccttacaa gctaaggggg agtcagttat tatcgaaaaa gagtacaccc gtaatcatac
     7621 tgaagatatg ttgcaacaat ttggtggtca tttaagtgtg gatggtaaga aaatcacagt
     7681 ccaagggcca caaaaattga caggacagaa ggtggtcgta ccaggagata tttccagtgc
     7741 agccttttgg ttagtcgcag gtttgattgc tccaaattct cgtctagtgc tgcagaatgt
     7801 ggggatcaac gaaactcgca ccggtattat tgatgtcatt cgtgccatgg gtgggaaatt
     7861 ggaaataact gaaatcgatc cagtcgctaa atctgcaacc ttgattgttg agtcttctga
     7921 cttgaaagga acagagattt gtggcgcttt gattccacgt ttgattgatg aattgcctat
     7981 tattgcccta cttgcgaccc aagcccaagg tgtaacagtt atcaaggatg ctgaggagct
     8041 caaggtcaag gaaacagacc gtattcaggt tgtggcagac gccttaaata gtatgggagc
     8101 agatattact cctacggcag atgggatgat tatcaaagga aaatcagctc ttcacggtgc
     8161 tagagtcaat acgtttggtg accaccgtat cggcatgatg acagctatcg cagccctatt
     8221 ggttgcagat ggagaggtgg agcttgaccg tgcagaagcc atcaatacca gctatcctag
     8281 tttctttgat gatttggaga gcttgattca tggctaaggt gttattaggg tttatggggg
     8341 ctggaaaatc gactattgca agaggcttgg accctaatta ccttgatatg gatgctctga
     8401 ttgagaagcg cctaggtatg tccattgcga attttttcgc tgaaaagaga gaagcagcct
     8461 ttcgtcaggt agaatcagaa gtcctagctg atttactaca aacagaccaa gtcgtgtcaa
     8521 ctggaggagg agtggttatt tctcagagaa atcgtgactt actcaagact aatacagata
     8581 acatctacct gaaagctgat tttgaaaccc tctaccaacg tatcgtagct gataaggaca
     8641 atcagcgacc gctttttcta aataatagca aggaagaact agtagctatt tttcaagaaa
     8701 gacaggcttg gtatgaggaa gtggctagtc gggttttgga tgtgaccaag ctaagcccag
     8761 aggaaattat agaggaacta agatgaaaat tgcttatcta ggtcccaagg gatcattttc
     8821 acaccacgtt gtgcagacag cttttcctca tgaggaattg caggcttttg ccaacattac
     8881 agatgtcatc aaggcttatg agcaaggatt agtggactat tctgtggtgc cagttgaaaa
     8941 ttctattgag ggtagtgttc atgaaacctt ggactatctt tttcatcagg ctcacatcca
     9001 agcagtagca gaaatcgttc agcctattca tcagcagttg atggtggttc caggccatac
     9061 taagattgaa aagatttttt cacatccaca ggccttggct caaggaaaga aattcatcga
     9121 tgaacaatat ccagaggctc aaatcgaggt aacagctagt acagcttatg cggcccgttt
     9181 tatttccgaa catccagatc agccctttgc agcaattgca cctagaagtt ctgctgaaga
     9241 atatggattg gaactgattg ccgaggatat tcaggaaatg gaagccaatt tcacccgttt
     9301 ctgggttcta ggagctgaaa agcctagtat tcccttgcaa gcacaaactg aaaagatgag
     9361 tttggccttg acattacctg acaaccttcc aggtgcactt tataaggccc tgtcgacctt
     9421 tgcttggcga ggaattgact tgacaaaaat tgaaagtcgt ccactcaaga cagcactggg
     9481 tgaatacttt ttcattatcg atgtggatta taccgataag gacttggtcc actttgccca
     9541 aaaagaatta gaagcgattg gaatccagta taaaattctg ggtgcctatc ctatttatcc
     9601 aatatcagac catggaaagg agagaagatg agtaaagaaa atcccttaag tcatcatgag
     9661 cagttgcgtt atgattattt gctaaaaaat attcactatc tcaatgagag agaaaaaaat
     9721 gagtttgtct atttgcaaga aaagctaact cttgctaggg gaaatagtag ctctagcttg
     9781 gaacaagaaa gagaagagca ggttgactta ccaagctatg cgaaccggag tcgctcacaa
     9841 tccaaatcac aagcactctc tttccctcca aaaaagaaaa gacggaagct ccgtcttaaa
     9901 cgaattttta tggtgatttt ttcacttttg gtctgtgtgg ctttggccat gatattcatg
     9961 tttttgcgtg gttaccaaga tgctagcgca aagaaaactg ctgatgcccg ggcagctcaa
    10021 gtagaagtct ttaatggtca ggacactaga gatggagtta atattttaat catgggtact
    10081 gatggtcgaa tcggccagaa cagtgttgag acacgaactg actctattat ggtattaaat
    10141 gtcgggggct cagataagaa aatgaagcta gtcagtttca tgcgtgataa tttggtctat
    10201 atagatggtt atagtcaagt gattaacggt agaaaacaga cagataacaa gttaaacgta
    10261 gcctacgagt taggagaaca agaggggcaa aaaggggcag aaatggttcg ccaagtcttg
    10321 aaagataatt ttgacttgga cattaagtac tacgccttgg ttgatttcca ggcctttgcc
    10381 acagcgattg acacactttt ccctgatggg gtgacgattg atgctcaatt ttcaacattg
    10441 aatgggcgtc ccctaacaga agctacagtc ggagatgatt tatatgctac cgagactgag
    10501 tctccaaccc aaaccattaa agtcggaaaa cagcagatga atggctcgac cctgctcaat
    10561 tatgctcgtt tccgtgatga tgatgaggcg gattacggcc gtacaaaaag acagcaacaa
    10621 gttttaacag caattttaga gcaaattaaa gatcccacta aacttttcac tggttctgaa
    10681 gctcttggaa aggtttttgc tatgacctca accaacgtac cctatacttt cctcctaaca
    10741 aacggtttat ctgttttgga tggagcaaaa aatggtattg aaaaattgac gattccagaa
    10801 ctgggtgact gggtagatgc ctatgatgtt tatggaggct tgggcttgct ggttgatcaa
    10861 aacaaatatc aaaccaagct cgctcaaatg ggcttgagat aagataatac tcaatgaaaa
    10921 ttaaagtgca aactaggaag ctagccgcag gctgctaaaa acacagtttt gaggttgtag
    10981 ataagactga cgaagtcagt tacctatacc tacggcaagg tgaagctgac gtggtttgaa
    11041 gagattttcg aagagtataa gacagaaaaa tcaaagtttg atagctattt ggatagcagt
    11101 caggctttga ttttttacag tctgcaatag attttcaccc cttgtttgtg gtataatgaa
    11161 acgatacgat agaaagaata atgaacaata tgactgattt aaaagcaatt caggctcgta
    11221 gtctggagat ggctgaatat tttgtggcct tttgtaaaga acatgatttg ctctgttatc
    11281 tctgtggcgg aggcgctatt ggtacccttc gaaacaaggg atttattcct tgggacgacg
    11341 acctagactt ttttatgcct cgtaaagatt atgagaaatt agcagaatta tggcctcgtt
    11401 atgcagatga acgttatttc ttgtcaaaga gttacaagga ttttgttgat cgtaatcttt
    11461 ttattaccat tcgtgacaag gaaaccacct gtatcaagcc ttatcagcag gatttggatt
    11521 tgccacatgg tctggccttg gatgttttgc ctttggatta ttatccgaaa gatccagctg
    11581 agcggaaaaa acaggttcgt tgggccttga tttattcact cttttgtgcg caaactattc
    11641 cagaaaagca tggtgctctc atgaaatggg gaagtcgcat tttactgggt ttgactccaa
    11701 aatctctccg ttatcgcatc tggaaaaaag ctgagaaaga aatgactaag tatgatttgg
    11761 ctgattgtga tggcattaca gaattatgct caggtcctgg ctacatgaga aacaagtacc
    11821 caatcacatc ttttgaagac aatcttttct tgccatttga aggaacagag atgcctattc
    11881 caatcggcta tgatgtctat ctcagaactg cttttgggga ttatatgacg cctccaccag
    11941 cagacaagca ggtaccgcat catgatgctg tcatcgctga tatggataag tcttatacag
    12001 aatacaaggg agaatatggt ggctaagaaa aaaatcttat tttttatgtg gtctttttct
    12061 cttggaggtg gtgcagagaa gattctatca accattgttt caaatctgga tctagaaaag
    12121 tatgatattg atattcttga aatggagcac tttgacaagg gatatgaatc tgttccaaag
    12181 catgtacgca ttttaaaatc ccttcaagat tatcgccaaa ccagatggtt acgagctttt
    12241 ttgtggagaa tgagaattta ttttccaaga ctggctcgtc gtttgcttgt aaaagatgat
    12301 tatgatgttg aagtttcttt taccattatg aatccaccac tgttgttctc taaaagaaga
    12361 gaagtcaaga agatatcttg gattcatgga agtattgaag aacttcttaa ggatagctct
    12421 aaaagagaat cacatagaag ccagttggat gctgcgaata caattgtagg gatttcaaaa
    12481 aagaccagca attctatcaa ggaagtttat ccagattata cttctaaatt acagacaatc
    12541 tacaatggat atgattttca gactattcta gaaaaatctc aagagaagat cgatatcgag
    12601 attgctcctc aaagtatctg tactatcgga cggattgagg aaaataaggg ttctgaccgt
    12661 gtagtggaag tgatacgatt attacaccaa gagggaaaaa actatcatct ctattttatc
    12721 ggggctggtg atatggaaga ggaactgaaa aaacgagtca aagagtatgg gattgaggac
    12781 tatgtacatt tccttggtta tcaaaaaaat ccttatcagt atctatctca gacgaaagtt
    12841 cttttgtcta tgtctaaaca agaaggtttt cctggagtgt atgtggaggc cttgagtctg
    12901 ggactccctt ttatctctac ggacgttgga ggggctgagg aattatccca agaaggacga
    12961 tttggacaaa tcattgagag caatcaagag gcagctcagg cgattactaa ttacatgact
    13021 tctgcctcaa actttgatgt cgatgaggct agccaattca ttcaacaatt tacaattaca
    13081 aaacaaatcg aacaagtaga aaaactatta gaggagtagc atggaaactg cattaattag
    13141 tgtgattgtg ccagtctata atgtggcgca gtacctagaa aaatcgatag cttccattca
    13201 gaagcagacc tatcaaaatc tggaaattat tcttgttgat gatggtgcaa cagatgaaag
    13261 tggtcgcttg tgtgattcaa tcgctgaaca agatgacagg gtgtcagtgc ttcataaaaa
    13321 gaacgaagga ttgtcgcaag cacgaaatga tgggatgaag caggctcacg gggattatct
    13381 gatttttatt gactcagatg attatatcca tccagaaatg attcagagct tatatgagca
    13441 attagttcaa gaagatgcgg atgtttcgag ctgtggtgtc atgaatgtct atgctaatga
    13501 tgaaagccca cagtcagcca atcaggatga ctattttgtc tgtgattctc aaacatttct
    13561 aaaggaatac ctcataggtg aaaaaatacc tgggacgatt tgcaataagc taatcaagag
    13621 acagattgca actgacctat cctttcctaa ggggttgatt tacgaagatg cctattacca
    13681 ttttgattta atcaagttgg ccaagaagta tgtggttaat actaaaccct attattacta
    13741 tttccataga ggggatagta ttacgaccaa accctatgca gagaaggatt tagcctatat
    13801 tgatatctac caaaagtttt ataatgaagt tgtgaaaaac tatcctgact tgaaagaggt
    13861 cgcttttttc agattggcct atgcccactt ctttattctg gataagatgt tgctagatga
    13921 tcagtataaa cagtttgaag cctattctca gattcatcgt tttttaaaag gccatgcctt
    13981 tgctatttct aggaatccaa ttttccgtaa ggggagaaga attagtgctt tggccctatt
    14041 cataaatatt tccttatatc gattcttatt actgaaaaat attgaaaaat ctaaaaaatt
    14101 acattagaac gggggtgacc aagtgattga tgggaaacga ttattattta gtttgaccat
    14161 agtcagttat gccttgacgc tagtaagtgg aattgtgtat ctgtttaata ataataatgt
    14221 tagcttactt tctactttat tgttcttgtt ggttagtagc ttaattgctt gttggaatga
    14281 tatcaagtat tacttaatcc attttatttt ctatttaacc atttttgtat ttctggtatc
    14341 aagaccgacc attgattatt ttagggatgg tgctttggat acctatcatc caatagccta
    14401 tcgttttgcc tttatagttg tcatgatttc gattctgggc ttgaccacag gaggcattct
    14461 ggctcgttac ttcatagcta ggaagaaaat aaaagtagca aatataggaa attctctaaa
    14521 agaggtttat atcaagcggt tacgctttgt atcactagga gtttttcttc taacttatcc
    14581 tttctatttc attcggttat ttgaacggct cttgtatcgt ttgcagactt cctactatgc
    14641 ctactatgca aattttgaaa gtaaactgcc ttattttacc tacattttgt ctacctttac
    14701 ggtctatgca atgtgtatgt atctggcaac caagccaaag aaattgcagg ccacagcagt
    14761 gcttgtctcc tttattgcag ctaatactat tcatttggca attgggacac gaaatccctt
    14821 tattttaagt attttatttg cttttgttta ttactttatg cgggagcaaa ctgaaaaagg
    14881 aaaatggatt gggtttaaag aaaagttagc gatttttgta ggttctccta ttctcatgtt
    14941 agcgatggga gtactcaatt atgtacggga taatgtccaa gtttcccata caggtttctg
    15001 ggatatctta cttgacttta tctataaaca agggactagt tttggtgttc tggctcgagg
    15061 ttttctattt aacagtagcc tcccttaccg agatttccgt aattttactt ttggtcctgt
    15121 tcttgattat tttgcaaggg ggagtttggg agccattttc ggaggaaaag cctttgaaca
    15181 tacaaccaat agtgtggaac tagctattga tagtaatagt tatgcccaca atctatccta
    15241 tcttgtcttg aacaaggaat acttgaaagg gcatggtatc ggaagtagtt atatcatgga
    15301 gttgtatacc gactatggta tgattggagt ctttctgctt agtttcttac tcggcgtatt
    15361 atttatagcc atgctgcaag tagcctatcg ctcaaggaca atcctatttg ctttatccct
    15421 actcatcttg aataatctat tctttatgcc aagaagcagc ttttcagaaa gtttcttcaa
    15481 tttatttaca atgcaattct ggggaattgt tcttgtgatt atatttgtag caaaaatgct
    15541 tacaaaggaa aactagtatc tactaaacaa aggagaaaaa aatcatgttt gaacattatt
    15601 cagtagctga tttgtttgca aatctttata aaaagcgaaa agcaaatatt ttagctctca
    15661 tcgctttatt tgctctcatt gctgtaccat ttacaattaa agcagttagg aataaaaaca
    15721 ctgtcaaaga tacaacaagt tattcaactt atcttatcta taaaatcact cctccaaaag
    15781 agtcggccaa aacgattttg aatcatcaaa ttggtggtta tagtgatttt tatgggaaat
    15841 tgattgatgg taatttgaat ggagcttatc ttttcaatga tgtagaaccc agtgagttga
    15901 aaaaaattgc cagtgaatta gacacgacag aaacaacctt gaaaaattca acgaatgact
    15961 attggtggaa aaaattgacc gtctactata tgattgacga tgcaggggtt ggtgtgaaaa
    16021 ttttgacatc aagtaaagat gccaatgact tgttagagaa aaaaattgat gggttgattg
    16081 agaaatttaa acatgcctat gcaaatgtga aaattgaaaa actggaaacc atcaactcta
    16141 aagaattgaa cgcaaatggt gaaacagcgc ttggcttaaa tgtgaaaaat ctgattcttc
    16201 gtttagttgt tattggagtg gtttgtgtga ttttggttgt gatgggaaat gtgttagttt
    16261 atctctttaa tccaacaatc aatagagtag gtgatttttc tcagtatcaa attgattttg
    16321 taacagagat tacaacaatt gctaacctag cagatgtttt gtcatacaaa aatactggac
    16381 aggaattgac catcgttagc tcaaataaag ctatcttaga taaattgaaa cagagtcaag
    16441 aagctttaaa aggaatgcat tttgtagatt tacaggatgt atcatctctt ttggaaagag
    16501 atacagtcct tcttgttgaa gagtacggag tgactcgtta taagaaattt gagcaaagtc
    16561 ttcaaattct cagaaactta aatcgttcta tcctcggtgt agcaaccttt aaattataag
    16621 tattctgatg tataaggtct tcaaaatcgg ctaagagccg attttgaagg ctttttctat
    16681 ctgcctgcag aaattttctt ttggagtctc ctatagaaag accctaggga aaatctatcc
    16741 gtatagagga gccttctttt gataaaatcg taaaaatcta gtataataga tagaactgaa
    16801 agtatgaggt tactagcaat gaaaatgaaa caaattagtg atacaacttt aaaaatcacg
    16861 atgtctttag aggatttgat ggatcgtgga atggagattg ctgactttct cgttcctcaa
    16921 gaaaaaacag aagagttctt ttatgctatc ttggatgagc tagagatgcc tgatagcttt
    16981 ctggatacag gcatgttgag cttccgtgtg actccaaaac ctgataaggt agatgtcttt
    17041 gtgaccaagt caaagattga ccaaaatcta gattttgaag acttatcgga tttgccagat
    17101 atggaagaat tggctcaaat gtctccagat gaatttatca aaaccctgga aaaaagcatc
    17161 gcagacaaaa ccaaggatga tatcgaagcc attcaatctc ttgagcaagt tgaagccaag
    17221 gaagaagagc aagagcaagc tgaacaagaa gctgagagta agaaagaacc ttacatctac
    17281 tacatccttt cttttgctaa gttggctgac ttggtagctt ttgccaagac agtgactttt
    17341 gaaatggaaa cttctgaact ctacaaaatg aacgagcgct attatttgac cattttagtg
    17401 gatattgaaa atcatccaag cccatatcca gcttggctgt tggcccgtat gcgcgagttt
    17461 gcagacgata gtgatatcag tcgctcagtc ttacaagagt atggtcaagt cttgatgagt
    17521 cacgatgcag tgctcaatct gcaaaaaatc ggctaatcct ttctggaaag ctattttcag
    17581 attttaagaa gagcgaatcg tctgattcgt tttttctttt tatactgaaa tagtgattta
    17641 ctataatagg aattttcaca aaattctgtt ataatggcta tattagaaaa tttcgaggag
    17701 ataaagatga cagttaaaat tgctttacta ggatttggta ccgttgcaag tggtgtgcct
    17761 ttcctcctaa aggaaaatgg aggaaaaatc aatcaatcag cacattcaga tatcaaagtt
    17821 gctaaggtat tggtcaagga tgaagatgaa aaaaatcgct tgcttgcagc agggaatgac
    17881 tttaactttg taaccaatgt ggatgatatt ttatcagacc aggatattac tatcgtagtg
    17941 gaattgatgg ggcgtattga gcctgctaaa acctttatca ctcgttcctt ggaagctgga
    18001 aaacacgttg ttactgctaa caaggacctt ttagctgtcc atggcgcaga attgctagaa
    18061 atcgctcaag ctaacaaggt agcactttac tacgaagcag cagttgctgg tgggattcca
    18121 attcttcgta ctttagcaaa ttccttggct tctgataaaa ttacgcgcgt gcttggagta
    18181 gtcaacggaa cttccaactt catggtgacc aagatggtgg aagaaggctg gtcttacgat
    18241 gatgctcttg cggaagcaca acgtctagga tttgcagaaa gcgatccgac gaatgacgta
    18301 gatgggattg atgcagccta caagatggtt attttgagcc aatttgcctt tggcatgaag
    18361 attgcctttg atgatgtagc ccacaaggga atccgcaata tcacaccaga agacgtagct
    18421 gtagctcaag agcttggtta cgtagtgaaa ttggttggtt ctattgagga aacttcttca
    18481 ggtattgctg cagaagtgac tccaaccttc ctacctaaag cgcacccact tgctagtgtg
    18541 aatggcgtaa tgaacgctgt ctttgtagaa tctatcggta ttggtgagtc tatgtactac
    18601 ggaccaggtg cgggtcaaaa accaactgca acaagtgttg tagctgatat tgtccgtatc
    18661 gttcgtcgtt tgaatgatgg taccattggc aaagacttca acgaatatag ccgtgacttg
    18721 gtcttggcaa atcctgaaga tgtcaaagca aactactatt tctcaatctt ggctccagac
    18781 tcaaaaggtc aggtcttgaa gttggctgaa atcttcaatg ctcaagatat ttcctttaag
    18841 caaatccttc aagatggcaa agagggtgac aaggcgcgtg tcgttatcat cacacacaag
    18901 attaataaag cccagcttga aaatgtctca gctgaattga agaaggtttc agaattcgac
    18961 ctcttgaata ccttcaaggt gctaggagaa taagatgaag attattgtac ctgcaaccag
    19021 tgccaatatc gggccaggtt ttgactcggt cggtgtagct gtaaccaagt atcttcaaat
    19081 tgaggtctgc gaagaacgag atgagtggct gattgaacac cagattggca aatggattcc
    19141 acatgatgag cgtaatctct tgctcaaaat cgctttgcaa attgtaccag acttgcaacc
    19201 aagacgcttg aaaatgacca gtgatgtccc tttggcgcgc ggtttgggtt cttccagctc
    19261 ggttatcgtt gctgggattg aactagccaa ccaactgggt caactcaact tatcagacca
    19321 tgaaaaattg cagttagcga ccaagattga agggcatcct gacaatgtgg ctccagccat
    19381 ttatggtaat ctcgttattg caagttctgt tgaagggcaa gtctctgcta tcgtagcaga
    19441 ctttccagag tgtgattttc tagcttacat tccaaactat gaattacgta ctcgcgacag
    19501 ccgtagtgtc ttgcctaaaa aattgtctta taaggaagct gttgctgcaa gttctatcgc
    19561 caatgtagcg gttgctgcct tgttggcagg agacatggtg accgctgggc aagcaatcga
    19621 gggagacctc ttccatgagc gctatcgtca ggacttggta agagaatttg cgatgattaa
    19681 gcaagtgacc aaagaaaatg gggcctatgc aacctacctt tctggtgctg ggccgacagt
    19741 tatggttctg gcttctcatg acaagatgcc aacaattaag gcagaattgg aaaagcaacc
    19801 tttcaaagga aaactgcatg acttgagagt tgatacccaa ggtgtccgtg tagaagcaaa
    19861 ataaagaata gaagatagga tggggaaact cttgaccaga gggtttcata tcctttttgt
    19921 gaaaagaagt ttatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaggctg
    19981 ctcaaaacag tgttttgagg ttgtggatag aactgaagaa gtcagctcaa gacactgttt
    20041 tgaggttgca gatagaactg acgaagtcag taacatatat acggtaaggt gacgctgacg
    20101 tggtttgaag agattttcga agagtattag ttaaaaacgt gataaaggag aaataaagat
    20161 ggcagaaatt tatctagcag gtggttgttt ttggggccta gaggaatatt tttcacgcat
    20221 ttctggagtg ctagaaacca gtgttggcta cgctaatggt caagtcgaaa cgaccaatta
    20281 ccagttgatc aaggaaacag accatgcaga aacggtccaa gtgatttacg atgagaagga
    20341 agtgtcactc agagagattt tactttatta tttccgagtt atcgatcctc tatctatcaa
    20401 tcaacaaggg aatgaccgtg gtcgccaata tcgaactggg atttattatc aggatgaagc
    20461 agatttgcca gctatctaca cagtggtaca ggagcaggaa cgcatgctgg gtcgaaagat
    20521 tgcagtagaa gtggagcaat tacgccacta cattctggct gaagactacc accaagatta
    20581 tctcaggaag aatccttcag gttactgtca tatcgatgtg accgatgctg ataagccatt
    20641 gattgatgca gcaaactatg aaaagcctag tcaagaggtg ttgaaggcca gtctatctga
    20701 agagtcttat cgtgtcacac aagaagctgc tacagaggct ccatttacca atgcctatga
    20761 ccaaaccttt gaagagggga tttatgtaga tattacgaca ggtgagccac tcttttttgc
    20821 caaggataag tttgcttcag gttgtggttg gccaagtttt agccgtccga tttccaaaga
    20881 gttgattcat tattacaagg atctgagcca tggaatggag cgaattgaag ttcgttctcg
    20941 ttcaggcagt gctcacttgg gtcatgtttt cacagatgga ccgcgggagt taggcggcct
    21001 ccgttactgt atcaattctg cttctttacg ctttgtggcc aaggatgaga tggaaaaagc
    21061 aggatatggc tatctattgc cttacttaaa caaataaaac agagagtggg gcttcccact
    21121 ttcttcattt ctagaatatg aatagaaggg atttatgaaa cacctattat cttacttcaa
    21181 accctacatc aaggaatcaa ttttagcccc cttgttcaag ctgttagaag ctgtttttga
    21241 gctcttggtt cccatggtga ttgctgggat tgttgaccaa tctttacctc agggagatca
    21301 aggtcatctc tggatgcaga ttggcctgct ccttatcttt gcagtaattg gcgttttagt
    21361 ggccttgata gctcaatttt actcagcaaa ggcagcagta ggttttgcta aggaattgac
    21421 aaacgatctt tatcgtcata ttctttcctt gcccaaggac agcagagacc gtctgacaac
    21481 ttctagtttg gtcactcgct tgacttcgga tacctaccag attcagactg gtatcaatca
    21541 attcctgcgt ctctttttac gagcgcccat tatcgttttt ggtgccattt ttatggctta
    21601 tcgaatctca gctgagttga ctttctggtt cttagtcatg gttgccattt tgaccattgt
    21661 cattgtaggg ttatctcgat tggtcaatcc tctctacagt agtctcagaa agaaaacgga
    21721 ccaactggtt caggaaacgc gccagcaatt gcaagggatg cgggttattc gtgcttttgg
    21781 tcaagaaaaa cgagagttac agatttttca aacccttaac caagtttatg ctagattaca
    21841 agaaaagaca ggtttctggt ctagtttatt aacacctctg acctatctga ttgtcaatgg
    21901 aactcttctc gttattatct ggcaaggcta tatttcaatt caaggaggag tgctcagtca
    21961 aggtgctctc attgctctta tcaattacct cttacagatt ttggtggaat tggtcaagct
    22021 agccatgttg ataaattccc tcaaccagtc ctatatctca gccaagcgaa tcgaggaagt
    22081 ctttgttgag gctccagagg atatccattc agaattagaa caaaagcaag ttaccagtga
    22141 tcgggtttta caagtccaag aattgacctt tacctatcct gatgcggccc agccttctct
    22201 gagagacatt tcctttgata tgactcaagg acaaatcctt ggtatcattg gggggactgg
    22261 ttctggtaaa tcaagcttgg tgcaactctt acttggactt tatccagtag acaaggggaa
    22321 cattgacctt tatcaaaatg gacgtagtcc tcttaatttg gagcagtggc ggtcttggat
    22381 tgcctatgta cctcaaaagg tcgaactctt taaaggaacc attcgttcca acttgactct
    22441 aggtttcaat caagaagtat ctgaccagaa actctggcag gccttggaga ttgcgcaagc
    22501 taaggatttt gtcagtgaaa aggaaggact cttggatgcc ctagttgagg caggggggcg
    22561 aaatttctca ggtggacaaa aacaaaggtt gtctatcgcc cgagcagtct tgcgccaagc
    22621 tccgtttctc atcctagatg atgcaatctc ggcactggat accattacag agtccaagct
    22681 cttgaaagct attagagaaa attttccaaa catgagctta attttgatct ctcaacgaac
    22741 ctcgacttta caaatggcgg accagattct cctcttggaa aaaggtgagt tgctagctgt
    22801 tggcaagcac gatgacttga tgaaatccag tcaagtctat cgtgaaatca atgcatccca
    22861 acatggaaag gaggactaga atgaaacgac aaactgtaaa ccagacgctc aaacgtttag
    22921 tcgtagattt agcaaaccat cccttcctcc ttttcctagc ctttctagga actattgccc
    22981 aagttggctt atcaatttac ctacctattc tgattgggca ggtcattgac caagtcctag
    23041 tggctggttc atcaccagtt ttttggcaga tttttctcca gatgctcttg gtggtaatag
    23101 gaaatactct ggtacaatgg gccaattctc tcctctataa tcgtctaatc ttctcttata
    23161 ccagagattt acgggagcga atcatccata agctccatcg tttaccgatt gcctttgtag
    23221 ataggcaagg tagtggagag atggttagtc gtgtaaccac ggacatcgaa cagttggcag
    23281 ctggcttgac catgattttt aaccaatttt tcattggtgt tttgatgatt ttggttagta
    23341 ttctagccat gctccaaatt catctcctca tgactctctt agtcttgctg ttgacgccac
    23401 tgtccatggt gatttcacgc tttattgcca agagatccta tcatctcttc cagaagcaaa
    23461 cagagacgag gggaattcag actcagttga ttgaagaatc gcttagccag cagactataa
    23521 tccagtcctt caatgctcaa acagaattta tccaaagatt gcgtgaggct catgacaact
    23581 actcaggcta ttctcagtca gccatctttt attcttcaac ggtcaatcct tcgactcgct
    23641 ttgtaaatgc actcatttat gcccttttag ctggagtagg agcttatcgt atcatgatgg
    23701 gttcaacctt gaccgtcggt cgtttagtga cttttttgaa ctatgttcag caatacacca
    23761 agccctttaa tgatatttct tcagtgctag ctgagttgca aagtgctctg gcttgcgtag
    23821 agcgtatcta tggagtctta gatagccctg aagtggctga aacaggtaag gaagtcttga
    23881 ccagtgacca agttaaggga gctatttcct ttaaacatgt ctcttttggc taccatcctg
    23941 aaaaaatttt gattaaggac ttgtctatcg atattccagc tggtagtaag gtagccatcg
    24001 ttggtccgac aggtgctgga aaatcaactc ttatcaatct ccttatgcgt ttttatccca
    24061 ttagctcggg agatatcttg ctggatgggc aatccattta tgattataca cgagcatcat
    24121 tgagacagca gtttggcatg gtgcttcaag aaacctggct cacacaaggg accattcatg
    24181 ataatattgc ctttggcaat cttgaagcca gtcgagagca agtaattgct gctgccaaag
    24241 cagctaatgc agactttttc attcaacagt tgccacaggg atacgatacc aagttggaaa
    24301 atgctggaga atctctctct gtcggccaag ctcagctctt gaccatagcc cgagtctttc
    24361 tggctattcc aaagattctt atcttagacg aggcaacttc ttccattgat acacggacag
    24421 aagtgctggt acaggatgcc tttgcaaaac tcatgaaggg ccgcacaagc ttcatcattg
    24481 ctcaccgttt gtcaaccatt caggatgcgg atttaattct tgtcttagta gatggtgata
    24541 ttgttgaata tggtaaccat caagaactca tggatagaaa gggtaagtat taccaaatgc
    24601 aaaaagctgc ggcttttagt tctgaataag ccattctctt ttgaaagttt atggacgaaa
    24661 aaagttgcct tcgagtgact tttttgttac aatagctaga aaaattgttc actgtaatac
    24721 tcaatgaaaa tcaaagagca aactaggaag ctagccgtag gttgctcaaa gcacagcttt
    24781 gaggttgtag ataagactga cgaagtcagt tcaaaacact gttttgaggt tgcagataga
    24841 actgacgaag tcagctcaaa acactgtttt gaggttgcag atagaactga cgaagtcagt
    24901 aaccacacct acggcaaagt gaatctgaag tggtttgaag agagtacaac ttgtctttta
    24961 aaaaaggagt ctataatgaa agtctttcag catgtaaata tcgtgacttg tgatcaaaat
    25021 ttccatgttt atcttgatgg aatcttagca gtcaaggatt ctcaaatcgt ctatgtcggt
    25081 caagataagc cagcgttttt agaacaagct gagcagatta tagactatca gggagcttgg
    25141 attatgcctg gtttggtcaa ttgtcacacc cattctgcaa tgacaggtct gagagggatc
    25201 cgagatgaca gcaatctcca tgaatggctc aatgactata tctggccagc agaatctgag
    25261 tttactcccg acatgactac caaggcggtc aaagaagcct tgacagagat gctccagtca
    25321 ggaacaacaa cctttaacga tatgtataat cccattggtg tggatatcca gcaaatttat
    25381 caggtggtga aaacttccaa gatgcgttgt tatttttctc cgactctctt ttcttcagag
    25441 acagaaacaa ctgctgagac tataagcaga actcgatcca tcatagatga aatcttaaaa
    25501 tataaaaatc caaatttcaa ggttatggta gcacctcatt ctccgtatag ctgcagtaga
    25561 gacttgctgg aagcgagttt ggaaatggca aaagagctaa atattccgct ccatgtccat
    25621 gtggcggaga ccaaggagga gtcaggaatt atcctcaaac gatacggcaa acgccccctt
    25681 gcttttcttg aagaactggg ttatttagat catccgtccg tatttgctca cggggtcgaa
    25741 ttaaacgaga gagaaattga acgcttggca tcttctcaag tggctatcgc ccacaatcct
    25801 atcagtaacc tcaaactggc atcaggaatt gctccaatta tccagctcca aaaagcggga
    25861 gtagcagtcg gaattgcgac tgactcggtt gcttccaata ataatctaga tatgtttgag
    25921 gaaggaagga ctgcagctct tcttcagaag atgaaaagtg gggatgccag ccagtttcca
    25981 atcgaaacag ctctcaaggt actgacaatc gaaggggcta aggtccttgg aatggaaaat
    26041 cagataggaa gtctggaagt cggcaagcaa gcagattttc tggtcattca accacaaggg
    26101 aaaattcatc tccaacctca ggaaaatatg ctgtctcacc tggtttatgc agttaaatct
    26161 agtgatgtag atgatgttta tatcgccgga gaacaggttg ttaagcaagg tcaagtcctg
    26221 acagtagaac tttaaaagaa aaattacgaa aaattttaaa aaaagtttgc aaaaatcttg
    26281 cattcttttt ttgactatgc tatacttata tacggtttaa aaaaactgcc taagacagta
    26341 ggggagctcg actcataaat atcctaccga ggacaaaacg tatcatgtaa aaagaagcgt
    26401 attgtacttt cgtgtctagg tttgggcgcg tttttctttt tgaaaaattc cccaagcaaa
    26461 ataattacgg aggtgaacac actaatgagt gaagcaatta ttgctaaaaa agcggaacta
    26521 gttgacgtag tagctgaaaa aatgaaagct gctgcatcta tcgtcgttgt agacgctcgt
    26581 ggtttgacag ttgagcaaga tacagttctt cgtcgtgagc ttcgtggaag cgaagttgag
    26641 tataaagtta ttaaaaactc aatcttgcgt cgtgcagctg aaaaagctgg tcttgaagat
    26701 cttgcatctg tatttgttgg accatctgca gtagcatttt ctaatgaaga tgttatcgca
    26761 ccagcgaaaa tcttgaacga cttttctaaa aacgctgaag cacttgaaat taaaggtggt
    26821 gcaatcgaag gcgctgtcgc atctaaagaa gagattcttg cacttgcaac tcttccaaac
    26881 cgcgaaggac ttctttctat gctcctttct gtacttcaag cgccagtgcg caacgttgct
    26941 cttgcagtca aagcggttgc agaaagcaaa gaagacgcgg cttaatctta agctacacag
    27001 cgtagcctag ctacgaaaaa aactattata aaatttaaaa cttatttgga ggaaataaca
    27061 atggcattga acattgaaaa cattattgct gaaattaaag aagcttcaat ccttgaattg
    27121 aacgaccttg taaaagctat cgaagaagaa tttggtgtaa ctgcagctgc tcctgtagct
    27181 gttgctgcag ctgatgcagc tgatgctggt gctgctaaag attcattcga cgttgaattg
    27241 acatctgcag gcgacaaaaa agttggcgtt atcaaagttg tacgtgaaat cactggtctt
    27301 ggtcttaaag aagctaaaga acttgttgac ggtgcaccag cacttgttaa agaaggcgtt
    27361 gcaactgcag aagctgaaga aatcaaagct aaattggaag aagctggagc ttcagttact
    27421 cttaaataat agagcaacta cattcgtagc ttaaaaacat gattaaaccg ctattcttag
    27481 gagtagcggt ttttcttttt gtttaaaaag gggcaaactt tttatatagt tgtttaggaa
    27541 ataaataatt tttgtataat tggtttacta ctctaaatta acgtagtaca agagtttgtt
    27601 aattaaacta taccttatgg ttttgactag tgtgatataa ttatgttgaa atacttatct
    27661 ggaaaggttt ttataatgag tttaagaaat aaaattgagc aacatattaa agagctagag
    27721 ggagggaagt ttcaaaaact aggtgacgcg tacctaagta gaaaatacaa ttttaatatt
    27781 gtttcattag gctcacagga aggaacagat aaaacaacta aagggatccc agattcttac
    27841 gctgtcgaaa atggtaaata tgtttatatt atgtatggca cccataagtc tgtaatatct
    27901 aaattagaag gagacattca gtcagttaag aaaaaaattt tagaggaaaa tattgctgaa
    27961 gataaagttg ggagacttat ttgttgtcat acttcttcaa atataacaat taaacaaaaa
    28021 gaagatctag aaaaaatggc tgagccttat catctagaat tgattggaat taatgaaatt
    28081 gctaatgatc ttacaaagat agattttcaa tatttagcaa aagagtatct atcaatcagt
    28141 gaatctacag agcaagtttg gtctatcaat gacttcataa gaattcatga tgagagtaaa
    28201 actaatgctc ctatatcaaa tgactatatt ggagatgtaa gtgaaattat aaataccatt
    28261 aaatcaagtg aaaaacgaat cttcttaata tctgctaagc ctggaacagg gaaaactaga
    28321 ttagcaattg aaatttgctc tttgctggat cgaaataaat ataacattat ttgtgtcaag
    28381 agcaataatc aggatattta ccaagatgta aaacgcaatc taaatttaca caaagaaaat
    28441 attgttttta ttgatgatgt taatactaca caaaattata tttctacact tgggctactg
    28501 aacacgacta gtaatattcg gtttattctt acggtacgtg attatgctaa aaaagatgta
    28561 atcaataata ttaaagttta tgggtacaat aatattgagt cagaacttat aaaagatgat
    28621 aattttaagg aattactcaa tcaattttct cgaaacgatt ttaccaatca agaaattgaa
    28681 catataaaaa cgatatctaa aagcaatccg cgtattgcag tcattgcagc aaagttatct
    28741 agctctcagg atttgactaa
//
LOCUS       DAVKLX010000028        27834 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKLX010000028 DAVKLX010000000
VERSION     DAVKLX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.028"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(249..2900)
                     /gene="adhE"
                     /locus_tag="VW325_001259"
                     /gene_synonym="adhC"
     CDS             complement(249..2900)
                     /gene="adhE"
                     /locus_tag="VW325_001259"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9734667.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELSFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(3200..3610)
                     /locus_tag="VW325_001260"
     CDS             complement(3200..3610)
                     /locus_tag="VW325_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734668.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            complement(3612..4040)
                     /locus_tag="VW325_001261"
     CDS             complement(3612..4040)
                     /locus_tag="VW325_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9734669.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            complement(4086..4385)
                     /gene="yajC"
                     /locus_tag="VW325_001262"
     CDS             complement(4086..4385)
                     /gene="yajC"
                     /locus_tag="VW325_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9734670.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            complement(4502..6478)
                     /gene="tkt"
                     /locus_tag="VW325_001263"
     CDS             complement(4502..6478)
                     /gene="tkt"
                     /locus_tag="VW325_001263"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9734671.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVISMPSTDVFDKQNAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            complement(6592..7683)
                     /gene="ulaG"
                     /locus_tag="VW325_001264"
     CDS             complement(6592..7683)
                     /gene="ulaG"
                     /locus_tag="VW325_001264"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9734672.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNSKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            complement(7795..9468)
                     /locus_tag="VW325_001265"
     CDS             complement(7795..9468)
                     /locus_tag="VW325_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9734673.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            complement(9645..10349)
                     /locus_tag="VW325_001266"
     CDS             complement(9645..10349)
                     /locus_tag="VW325_001266"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9734674.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            complement(10351..11214)
                     /locus_tag="VW325_001267"
     CDS             complement(10351..11214)
                     /locus_tag="VW325_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9734675.1"
                     /translation="MVRPIGIYEKATPTHCTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            complement(11218..11883)
                     /locus_tag="VW325_001268"
     CDS             complement(11218..11883)
                     /locus_tag="VW325_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9734676.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            complement(11900..12385)
                     /locus_tag="VW325_001269"
     CDS             complement(11900..12385)
                     /locus_tag="VW325_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9734677.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            complement(12461..12742)
                     /locus_tag="VW325_001270"
     CDS             complement(12461..12742)
                     /locus_tag="VW325_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9734678.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            complement(12765..14222)
                     /locus_tag="VW325_001271"
     CDS             complement(12765..14222)
                     /locus_tag="VW325_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9734679.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDREKYYNGEVQEEA"
     gene            complement(14363..14986)
                     /locus_tag="VW325_001272"
     CDS             complement(14363..14986)
                     /locus_tag="VW325_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9734680.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQCLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQTKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            complement(15037..16023)
                     /locus_tag="VW325_001273"
     CDS             complement(15037..16023)
                     /locus_tag="VW325_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9734681.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            complement(16042..16866)
                     /locus_tag="VW325_001274"
     CDS             complement(16042..16866)
                     /locus_tag="VW325_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9734682.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            complement(16841..17212)
                     /gene="rnpA"
                     /locus_tag="VW325_001275"
     CDS             complement(16841..17212)
                     /gene="rnpA"
                     /locus_tag="VW325_001275"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9734683.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            complement(17361..18551)
                     /locus_tag="VW325_001276"
     CDS             complement(17361..18551)
                     /locus_tag="VW325_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9734684.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            complement(18602..19555)
                     /locus_tag="VW325_001277"
     CDS             complement(18602..19555)
                     /locus_tag="VW325_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9734685.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGETEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            complement(19616..20209)
                     /locus_tag="VW325_001278"
                     /pseudo
     CDS             complement(19616..20209)
                     /locus_tag="VW325_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(20346..20759)
                     /locus_tag="VW325_001279"
     CDS             complement(20346..20759)
                     /locus_tag="VW325_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9734686.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKKSEEKPEKKENS"
     gene            complement(20737..21198)
                     /locus_tag="VW325_001280"
     CDS             complement(20737..21198)
                     /locus_tag="VW325_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9734687.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRFYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            complement(21161..21421)
                     /locus_tag="VW325_001281"
     CDS             complement(21161..21421)
                     /locus_tag="VW325_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734688.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(21426..21830)
                     /locus_tag="VW325_001282"
     CDS             complement(21426..21830)
                     /locus_tag="VW325_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9734689.1"
                     /translation="MIKAFTMLESLLALSLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLPVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(21823..22089)
                     /locus_tag="VW325_001283"
     CDS             complement(21823..22089)
                     /locus_tag="VW325_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
                     /protein_id="HEU9734690.1"
                     /translation="MLVVLLIISVLFLLFVPNLTKQKEAVNDKGKAAVVKVVESQAEL
                     YSLEKNEDASLSKLQADGRITEEQAKAYKEYHDKNGVANRKVND"
     gene            complement(22091..22348)
                     /locus_tag="VW325_001284"
     CDS             complement(22091..22348)
                     /locus_tag="VW325_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734691.1"
                     /translation="MKILNIELANVEQTDLGFEHWVDVTYQVPILKNEYTVKLLLLME
                     CRIEDQEVIEYLVSTWKYRDLVLHSVKMYEIEKSESFTILD"
     gene            complement(22603..23559)
                     /locus_tag="VW325_001285"
     CDS             complement(22603..23559)
                     /locus_tag="VW325_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9734692.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNTESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTDDLEGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     SWKKYTDGNWYWFDNSGEMATGWKKIAEKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFVQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(23563..23895)
                     /locus_tag="VW325_001286"
     CDS             complement(23563..23895)
                     /locus_tag="VW325_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEU9734693.1"
                     /translation="MQQITEIITNGAISILVILAGIAVKAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            complement(23899..24198)
                     /locus_tag="VW325_001287"
     CDS             complement(23899..24198)
                     /locus_tag="VW325_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734694.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHAVGDIQKEMVRMNDRMEEIEKGE"
     gene            complement(24208..24558)
                     /locus_tag="VW325_001288"
     CDS             complement(24208..24558)
                     /locus_tag="VW325_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734695.1"
                     /translation="MAETNQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQLKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTGEPKE"
     gene            complement(24561..24764)
                     /locus_tag="VW325_001289"
     CDS             complement(24561..24764)
                     /locus_tag="VW325_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734696.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITNNILSIQLDEQREGNQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(24745..26127)
                     /locus_tag="VW325_001290"
                     /pseudo
     CDS             complement(24745..26127)
                     /locus_tag="VW325_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
BASE COUNT         7798 a         6181 c         5010 g         8845 t
ORIGIN      
        1 aatcgatcag gacagtcaaa tcgaattcta acaatgtttt agaagcagat atgtactatt
       61 ctagtttcaa tctgctatat attttgtttc tattctagtt tttcaaaaag ggcaaaaagg
      121 cctcaacatc ataaaaaggc atcctatcag gtattcaaaa accttgatag gatgcctttt
      181 atcatagaaa aattactgga tctttccctc aaattgagtt tttgtcttgc ttctagactg
      241 ataaacaatt atttacggcg tcctggtctt tctttgtagc catagtatgc atcttcgatg
      301 atttcttgca tatggtctac cattggaaga cgtgggttag ctggtgaaca ttggtcttca
      361 taagccagga aggataattc acgagaatgt tctttccatt ctttttcgtc aattccttgg
      421 tctctaaaat tcatttggat cccaatacgt tcaccgagtt cgtagacagc ttttgcgtaa
      481 gattcaaccc cttcttctgg agtagaagct ggaagtccaa gcatgcgtgc gatatcttgg
      541 tatttttcat ctgcacggta gtagttgtac ttaggccatg ttgctgtctt agctggacgt
      601 gtaccgttgt aacggataac gtatggaagc aagatagcat ttgtacgacc gtggattgtg
      661 tggaattgcg caccaatctt atgggccatt gagtgagaaa tacctaggaa ggcattggca
      721 aaggccatac cagcgattgt tgaagcgtta tgcattttct cacgtgagtg gaagtctgca
      781 ttcttaactg agctttcgag attttcaaag accaatttaa tggcttgaag tgctaaacca
      841 tcagtgtagt cactagccat ttgtgatacg tatgcttctg tcgcgtgagt caatacgtcc
      901 ataccagtat cagcagcaac aaatcctgga actgtcaata ccaaagcagg gtctacgatt
      961 gccacagttg gtgtcaatga gtagtcagcg attgggtatt tacggttgtt tgctttatca
     1021 gagataacgg caaatggtgt tacttcagat cctgtaccag atgtagttgg aatcgcgatg
     1081 aatttagtct tcttaccaag caatgggaac ttgaaggcac gtttacggat atccatgaat
     1141 ttttggacaa ggtcacggaa gtccacttct ggttgctcgt agaagagcca cattactttg
     1201 gcagcatcca ttggagaccc accaccgagt gcgatgatgg tatctggttt gaaggcacgc
     1261 ataatctcag taccacggtt tacagttgtg atatctggat ccggttctac atccgcaaag
     1321 atttggtaaa caaccttatt gcgacgaagg tccagttgtt cgatgatacg atcaaggaaa
     1381 ccaagctcta ccatggcatg gtcagtaacg atcatgacac gttcaacgtc acgacatttt
     1441 tgaaggtatt gaattgaatc acgttcaaag tatgtttttg aaggaagttt catccattgc
     1501 atgttatttc tccgtcttcc gacttttttg atattcaaga ggttaatggc actaacgtta
     1561 tccccaactg agttgcgtcc gtaagaacca catccaagtg tcaatgatgg caagaaggca
     1621 ttgtaaacgt ccccgatacc accaaaagta gaaggtgagt tacagataac acgaatagct
     1681 ttaacagctt taccaaattc tttagtcaat tcttcgtcag ctgtgtggat agctgctgag
     1741 tgtccaagac cgttaaattc aaccatttga cgagccttag taataccatc ttcacggctt
     1801 tcagatttca aaactgcaat aactggtgac aatttttcac gagtcaatgg ctcattttcg
     1861 ccaacttctt tacattctgc agcaagaatg tttgttcctt ctggaactgt aaatcctgct
     1921 tgttctgcaa tccaagttgc tggtttacca acgatgtcag cgttcaattt tgcaccagca
     1981 cagtttttgc tgtttgcttt gacgccgaag cagaactctt caagaagagc tttttctttt
     2041 ttgtttacaa agtaagtgtg gtaagatttg aactctgcta caaattcatc gtaaatttct
     2101 ttatcaatga taactgcttg ttcagatgca cagaccatac cgttatcaaa tgatttagac
     2161 atgacgatat cgtgtgctgc ttgacgaatg tttgctgatt tttcaacata agctggaacg
     2221 tttccggcac ctaccccaag agctggttta ccacatgaat aagccgcctt aaccatggca
     2281 ttaccacctg ttgcaaggat tgtcgcaaca ccttcgtggt tcataagggc acttgttgct
     2341 tccatagatg gttgagtaat ccattgcaca cagttttcag gagcaccagc tgcgatagct
     2401 gcatcgcgga cgatacgagc tgcatgagca gatgattctt gtgctgatgg atggaaggca
     2461 aagacgattg ggttacgtgt cttcaatgaa atcaatgatt tgaagattgc tgttgatgtt
     2521 gggtttgttg ttggagtaat accacaaaca acaccaactg gttcagcaat aagagtcaat
     2581 cctgttacat cgtcttcttc gataacgcca actgtcttag tgtggcgcat attgtttact
     2641 acgtgttcac aagcaaacaa gttcttagtt gctttgtctt caaatacacc acgtcctgtt
     2701 tcttcaaagg catgtaaagc caattctccg tgggcatcca aagctgctac tgatgctttg
     2761 gcaacgatgt agtcaacttg ttcttgatcc aatttacgca tttcttcaag ggcaactaga
     2821 gctttttgaa ccaactcatc tacgtgtttt tcagcaacga gtttcttttc ctctggtgtc
     2881 acagtttttt tatcagccat attttcctcc atagctttca aggaacgata tcctttgtta
     2941 attttttcac aagtttatta taacgcatta tttcaacttt gtaaacagtt tcataaacat
     3001 ttcacaaaga atttttaaac tcttgtgaaa cttttctcat tttcttttct tatcaagttt
     3061 tatctttgaa gtttgtgaaa tttatttcaa atatttttta tttcacaaac ttgcttggaa
     3121 gagggatttg gaaagaaagg gatttacagg taagcgctta ccttgtgatt ctaataatac
     3181 ctcctttgat aaggaggctt tatttttgtt gaaaaacgcc ttgtttaaaa gttccttcat
     3241 aaacgacttc ttgttctgtt gttagtttcc cttttccttc agcctgacca tttacaaaat
     3301 caccttcgta ggtccagcct tctttagatt gaaaggtacc ttttccgttg aaggctccat
     3361 tgttgaagcc acctgtatat tggtctccat tttggaaggt aatggtacct tggccattca
     3421 ttttaccacg gacaagactg ccatcataaa caatcgttcc attatcgagt tttaagacac
     3481 cttttcctgg aatatttaga aaaaagacga gtacagcaca gaaaacaatg gtaactactg
     3541 ccaaaagctc taaacgtgga cgagtcagat agacacgata cttttcgtaa aaattcgtaa
     3601 gattttccac cttaactctc cttttctaaa cgttctaacc aagcttgaca gccctcttga
     3661 acacgtcgat aggtttcttc aaaatctcct gtataccaag ggtctggaac actttcagat
     3721 gaaaatgagt aaatcttatc ttgacagtct actggacaca tctgacgtaa gtcagaaata
     3781 tttgaagcgt ccattccgat aatataatca aaggcttcaa aatcttcctt actaatctga
     3841 agcgatgtct tgttcttgtc ataaggaatc tcatactctt gaaaaattcc ctgagtcccc
     3901 ttatgaatcg gattgccatg ttcccaagag gaagttgctc gactttggat ttcgtagtta
     3961 tctgtcattg atttcataac aaactcggcc atagggctac ggcaaatatt tcccagacag
     4021 acaaagacta attttttcat cccatttcct ttcttttata gaaaacggga gttcgtgatc
     4081 ccgtcttatt tttcaattgc gccttctagt gaagctgttt ctttcagcgg taatactgtc
     4141 ttgatagcag ctagttcaaa agtcaagtaa actccatcta cgtcaagaac tattgttccc
     4201 ttctccgtat ctacttcatc gactgttcca taaagtccac cgattgtaat cacttcatag
     4261 cctttttgta gtttatttaa gctttccata cgcttttgag cttgtttctt ttgagaacgt
     4321 tgcataaaga acatcaaggc catcatacct acaagcatga ttaaaaaagt aatatttgga
     4381 ttcattgttt tctcctttgt cttttacata ggactactat atcaaatttc agctactttt
     4441 acaagattgt accttacttt tctggtaaga aaaaccagag cttacgccct gatttttagg
     4501 attatttcaa atttcgaaca acttttacaa gattttcgac agtaaagcca tattctgcca
     4561 atacttttgg tgctggggca gaggctccga aggtatcaat acctagaacg gcaccatcga
     4621 gaccaacata tttgtaccag ttttgacttg cacccatttc gactgcaaca cgacggcgga
     4681 ctgcatttgg aagaatctct tccttgtaag ctgcattttg tttatcaaag acatctgtag
     4741 atggcatgct gattacgcgg attttttcgc cttgactagc caattcttta gcagctgaga
     4801 cagcaagatt aacctctgaa cctgtcgcaa tcaagatggt atcaaagtcg gctgcatttt
     4861 catatacaac ataagcacct ttagcaacct tgtcgaagtc tgttccatct tcaacagtca
     4921 agttttgacg tgtcaagaca agggcagttg gtgttttctc acttgtcact gcaaggtacc
     4981 aagctgcttg cgtttcacgc gcatctgctg gacggaaaac atttagattt ggcatagcac
     5041 gaagacctgc taaatgctca actggttcgt gagtcggacc atcttcccca actgcgattg
     5101 aatcatgggt aaagacataa gtcacaggaa gtccttgtaa ggctgacaag cggacagctg
     5161 ccttcacata gtcagagaag acgaagaaag ttccaccgta tacacgaagt ccaccatgaa
     5221 gggccatccc gttcaagatt gttcccattg caaattcacg aacaccaaac tgaatgttac
     5281 gattcaagcg attggtatcg tcttgaagtc catccgtctt gatataagtc atgtttgagt
     5341 gagcaaggtc agctgatcca cctaggaagg ttggtaattt agctgccaca acattcaagg
     5401 catcttgact tgaattacga gttgcttgag aaaaaccatt ttctaaagct gggaagtctg
     5461 ctggagtcac ttcgactgga tcacgtccgt cgatgatggc ttctacttct gcagccagtt
     5521 ctggatgagc ttctttataa tctgcaacta atttagtcca agcttgataa gctgatgcgc
     5581 cacggtctgc aacatgttct ttgaaatcag catatacttg ttctggaatt tcaaatggtt
     5641 cgtagtccca accgagggct tggcgagttg atgcagtttc atctgctcca agaggggcgc
     5701 cgtgtacagc attagttcct tgtttgtttg gagaaccata tccaataacc gtcttcactt
     5761 caattaaaga tggcttgcct gaagcttttg ctgtttcgat agcagcatgg atggcttcca
     5821 agtctgttcc attttcaacc aaggcagtat gccaaccgta ggcattgtaa cggtcacgaa
     5881 cactttctgt aaaggaatcc tttgtctcac catccaagtt gatatcattt gaatcataaa
     5941 gaacaaccaa cttatcaagt ttttgcaaac ctgcgtatga agctgcctcg cttgagacac
     6001 cttccatcaa gtctccgtct ccacagataa cgtaagtata gtggtcaaag atattgtagc
     6061 cttcacggtt atatttggct gccaagaaac gttctgcttg ggcaaaacca gtagcagttg
     6121 aaatcccttg ccctagagga cctgtcgtag catcaatccc tgccgtatga ccaaattctg
     6181 ggtgacctgg tgtttttgaa ccccattgac ggaaactctt aatctcatcc atgctgacat
     6241 cttcaaaacc agaaaggtga agaagagcat aaaggagcat tgaaccatga cctgctgaaa
     6301 gaataaagcg gtcgcggtta atccaatttg gttgagctgg attgatatga agttgttttg
     6361 taaagaggct gtaagccatc ggagccgctc ccataaccac acctggatga cctgagttgg
     6421 ctttattaat ggcgtcaata cctagaaaac gaattgcatt aacagataga tttgacatag
     6481 aatttccttt ctatttgagg tgattattga atcacacatt ctattttact attatatgaa
     6541 aattatccgt atcatgcaac atacggataa cctccaaaga atatttttat attatagcaa
     6601 agctttaaat tgaatgttag agtcttgttc aaaacaatca tcaaaaccac gtggatgatg
     6661 gtattctact aagtgttgat cttgaggata agtgtactta ccgccaactt cccagataaa
     6721 tggatggaaa tcgtattgca agcgatcttt tcgcattttc caaagttcta gaatctcatt
     6781 agtagaagcc atgaagttag accagatatc atagtgaact gggataatga ctttggtacg
     6841 cagattttct gccatacgaa gaagatcgat agatgtcatt ttgtcttgga tacctaccgg
     6901 attttcacca tagttattca aagcaacatc aattttaaag tctttaccat gttttgcaaa
     6961 atagtttgag aagtgagaat ctgcaccatg atagatggtt ccacctggtg tttcaaagat
     7021 atagttaaca gccttttgag ccatttcttc atctgtaaca gccaagccag caagttcacc
     7081 gcctgtctca tcagcaccgt tcactgggag agttaccaag caagtacggt caaatgattc
     7141 tactgcatga atcttcatat cttttaattc gatagtgtca cctggtttaa caacgatgat
     7201 acgttctttt ggaacacccc atccttccca gattcgtcca cagtggtaag gaccgataaa
     7261 cttaacatgc tctaacttag aattgttgag aattgctgca gctgtgtatg ggtcgatatg
     7321 atcactgtgg aagtgtgaaa ctaagtaata gtctagttcg ttgatagcaa atggatcgat
     7381 aaccattggc tgaacacgca agtttggttg caacttacga acacctgcca tatttgccat
     7441 ttggtgccca cgaaccatat ctttcacttt tttggttgat tttccacggt ttgaccaaag
     7501 gtccatgaca acgttagcac cagctggtgt cttaatccaa gtaccacagt tgcctagcca
     7561 ccacatggca aaattgcctt caggtacgac ttcttcttcg atttcttcgt tcaaccatgt
     7621 tccccactct gggaaagtgg ctaaaatcca tgactctctt gtaatttctt tgacgtttgg
     7681 catatgttgc ctcctttgga tataattgtt atctttgaga ttagtatacc acccttcaaa
     7741 acactaaaaa agaccaagaa aaacacaggt ttgtgttcat cattggactg tccttcaaat
     7801 atcttccaaa aaagcttgaa ctactatttc ttgatttata agtgtttgaa cctcttcttg
     7861 gaacttacta gccaacttgc tgtctacaat ataagacgaa ataagactag aaagattttc
     7921 actgaaactt ttgctcttat tacgaaatat attgtgttta agatagtcta gcattttcaa
     7981 aatatcttct gcttcaagga taggattaac ccttaaaatc ggaaatctgc tatccaaatc
     8041 atcattagta gtaatcacta catcaacttg tgcaatatct tccacactct taaattgttc
     8101 tgttgtaaat acagtgtcaa tttgctcatt tggaaaataa agtttgcatt gtttcagcaa
     8161 aagtctcgaa accgcaacac cttcatcaca aacgagataa acttttttca tatttttttg
     8221 agacgatggt gtatacttta aaaatcctcc aatatgaatc gtcaaatagg caatatcatc
     8281 gtctgttagc cgaataaacc atgctccttc taaaatttcc gcagattttc tagtgactaa
     8341 aaataattct ccatacttgg atcgaatttg ttttgttaga ggatttttag aaaaaatacc
     8401 gtaagtcttt ctaaataaca aggctttaca gtggatcatc aaatttcgta acaaatcatc
     8461 cttgttctca atctccattc ggatttgtga ttcaaaatac cagataaatt cttctaaagc
     8521 aagttttaat tgccgaaaat catcactttc tgcatgaata tccaaatcct tacgatagga
     8581 gaggaggaga acagctacaa gagaaacttc aagtcctgac aaagaaattt caaacttttg
     8641 aaacaaccgt tctcctaatt tcttagacac ctgatactct attctttttc ttatcaaaga
     8701 aaattctttc tctatatcct gatgtctttc ttgatactgt tcaacattat gacagcttaa
     8761 cagcaaataa ggtagaacct gcaacataaa agttatttca tgatggttta ttttcttccc
     8821 taaatcttgt tcaactaaag gaacctgttc cttaaaaaat tggttcattt caacagaaag
     8881 caaacactca tcatctaacc tctctttcat cttatcttct aaaatagata caaaagtggc
     8941 attttcttcc ataaaaatat gatatagaag cgattgaaga tactgaattt tattaagagg
     9001 gtgggcatga aggtggtatc cctgtgactt gctcacttgc aagatcacct gatattgttc
     9061 caaagttagt tgataacgaa tactattcaa atcattgaga acagtattcc tagatacctc
     9121 tgttaactct atcaattttt caatcgtaat acgttcttta gaaataccta tccatagtaa
     9181 cattatcatc atacgttcat gcgcactcat gatataatcg tacgaatcta cttccgataa
     9241 tagtttacaa caagcatctc tctgctcttc cgttaagtga ataccaattc gtggaatact
     9301 aatgatgtga agcgcctcgt caaccagagc agcattgatt ttgtcaatgt gataataaat
     9361 tttccttctt gactctttca aatctttcga aatcgccata atcgttttgg acgtttcttg
     9421 atccatcaaa tattgaagca aatcacaact tgccttatcc agtaccatat taaaaaatcc
     9481 cccaaatcta ttgatatcac agcaattctt tttattttac catatatatc aagcgctatt
     9541 tctaaaaaat ctttcaaaat tacttcaact ccaactttac tctcaataaa aatcaaatgt
     9601 aaaaagagga gctaaattta tctttttctc ctccttcatc gttcttactt ttgaccataa
     9661 taagcatttg gtccatgttt acgttggtag tgtttttcta gtatgtactg gggagcaggt
     9721 ccaactcttg gattgatttg ttctgtaaag cgattcatct ttgatacttc ctctagtacg
     9781 acagagtgat aaacagcatt ctctggattt ttgccccagg tgaatggacc gtgattgcgt
     9841 acaacaattc ctggtacttc aaccgggtta agtccgcgat gttcaaattc ttctacgata
     9901 accaggccag tatctttttc ataggccact tctacttcgt ccttggtcaa actacgggcg
     9961 caagggattg aaccgtagaa ataatctgca tgggttgttc cgtagaaagg aatatcacga
    10021 cctgcctgag cccaaccaac agcttctgtc gaatgggtgt gaaccacact accaatttct
    10081 gaccaagcct tatataattg cacatgagtt gggaggtcgg aagatggtct taaatcccct
    10141 tctaggatct taccatctag atcagtcact accatgtttt caggtgtcaa ttcgtcataa
    10201 tccacgcctg atggtttgat aacaatgaca ccgagttcgc gattgacttc agatacattc
    10261 ccccaggtaa atttgacaag tccatgtttt ggcaatgatt gattggcatc acaaactcgt
    10321 ttacgcatag cattgattac ttgattcatc ttacatcaaa cctgctttct taatgagtgg
    10381 atagagaaaa gcttgcgcct cttgaatggc tgcgcgtgtt tcttctactg tttcacaatt
    10441 ttcagaccac atttcgatta ggaaaggtcc attataattg gtttccttta aaatatcgaa
    10501 agcttcttcc catttgacac aaccttgccc aaaaggtaca tctcggaact ggccctttga
    10561 actttctgtc actgcataag tatccttgag atggagagct gcgatggcat gatgaccaag
    10621 ataaaactca ctatagatat cattatgcca tgcagacaca ttaccaatat ctggatatac
    10681 aaagaggaag ggagagtcaa tctctttttc tatagccaaa tatttttcga tgctattgat
    10741 gaaaggatca tccataattt caatagcaag taccacctga gcttcttcag cccagtcaca
    10801 ggcttttctc aaatttttga taaaacgttg gcgtgtctgg ggtgactttt cctcatagta
    10861 aacatcgtaa ccagctaatt gaatcgtacg aactcccaag tcttgagcta attcgataca
    10921 ttttttcatg agttctagag atttttcctc tagaactgga tcttttgaac ccaatgggta
    10981 gcgacgatgg cctgaaaaac agatagaagg aatacgaaca ccagtttcat agattgcttt
    11041 gacaacttcc aagcgttctt ccttactcca gtcaagtctt gctaaacgct cgtcacgttc
    11101 gtcaatagac atctcgacaa aatcaaagcc taactccttg gcaaaattta aacgttctag
    11161 ccaagtacag tgtgttgggg ttgccttttc ataaattcca attggacgta ccatgattta
    11221 cccccaaatt cgtttgattt catccttgaa ggcacgcgct gctcctgctg gatccgcagc
    11281 ctctgtaatt ccacgacctg cgataaaggt aaagacatca acaccttcaa agagtttgag
    11341 agtatctaca tctagaccac ctgttacaga tacacggaag cccatgtcaa tgagtttttt
    11401 aaccttatta aggtcttttt caccccaagt ttcaccagca agaagagcat cacgagattg
    11461 gtgataaata gcttgtgaaa tacctgcatc tagccaaagc tgagcttgtt caaaagtcca
    11521 atcgccataa agctcgatct ggatttcgcc tcgttctcct cgttcagtct tgatagcctt
    11581 tagagctgct tccatagtag ggatggttgc acaacagata caagtcatcc agtctgctcc
    11641 acgaaccgca ttatttttag caactgttcc accagcatca gcacattttg tgtctgccac
    11701 aataatctta tctgggaaaa ggctacgcaa gacttcagcc agttcacttc caacttgaag
    11761 caagcaaaca gttccagctt cgataatatc tacttcctga ccaacagaaa cagctgcttt
    11821 aatcgctcct tgcaagtctg aatggtctaa tgcaacttgt aaattaggta ttctttttgt
    11881 catttcttta ttcctctttc taactttcca aatccaatcc ttcgagataa gggctatcct
    11941 tagattcttc aatcatagcc aagacatctt ctttagtctg gcaagcctgt aaacgtgcaa
    12001 tagaatcttc taattcaaac agggcaataa tttgtggaat ggctacactt gtgtgaattt
    12061 ttgaacttgt tgctgctagt gccaacaaaa cagatacctc tttcccatct gaaaatacaa
    12121 caggattttg taaggtaatc aatgaaaagg catcactttg cacaccagct tcaggtctag
    12181 cgtggggcat agccatacct ggcatcaaga tatagtaagg cccatactct tcagtcgatt
    12241 caatgatagc atcgtaatac tctggcaaaa ttgccccact ttcaattaag ggatctactg
    12301 ctaccttgac tgcttctttc caattgttag cctctaaacc tagtcggatc gagtcattgt
    12361 caattaaagc ttgttttaaa ttcatatctg cctcctttat ttaaagaggg agaaggacag
    12421 aaacgataac tctcagtttc tgtccagccc ctccaacctt ttactgtagt gcttgactga
    12481 gtttttcggt gatttcttta tcatccatca agttatcaag cccaattaac ttcccattag
    12541 ttcgcccttc caattcttga atcaaatgaa gagaagcgat tacgatgtca tatcctactg
    12601 ctaaaccttt agcttcaccg acactgcatg aattgactgt aaaatctgtt tgattaagct
    12661 tacggagagc attttcaacc ttcatcttga taaccattga tgaacccatt ccatttccgc
    12721 acgctgctaa tactttaacc attttatttc tccttttcta gatactaagc ttcttcttga
    12781 acttcaccgt tgtaatattt ctctctatct tttgctttgg caaattgaag ttgaggaata
    12841 acaagcaaga agagacacac aagtacataa ccaacaatac caaggtattt gaagatatat
    12901 ccaaatccaa gccatgggaa ttcaaagtcg atatttccat ggtagccacc ataagatgcc
    12961 aaatcgagaa gggccacaca aagagctcct agagcaactt gaaggacacc tgatataaag
    13021 gaaaggataa cagccgcttt ccatccgccg cgtttatcag cgtagaccgc aatggctgca
    13081 ttgtcaaaga acactggtac aaatcctgta ataataagaa tcggattttt aaagacgatg
    13141 agcaagacaa ttgtaatcaa ttgaccaatc aaaccaaagg taaatcctga caagacagca
    13201 tttggagaac caaatccata agaagctgca acgtcaaccg ctgggaatga acctggcaac
    13261 aatttgtttg aaataccttg gaaggcgttt gtcaactcag atacgaacat tcggacacct
    13321 tgcatcaaaa cgaacaagta aactgagaag gtaaaggctg tttggataat gtacataaag
    13381 aaatcttgtt tagcaggatt gaatagagtt cctgaagtga tgacttcttt attagacata
    13441 atgtctggac ccaagattaa aagaatggct ccgaagaata cgagcatcaa ggtagcagat
    13501 gcaacaactg tatcgtggaa gattgagagg aacttaggta atttaagatt gtctaaactt
    13561 tcttctttct taccaaagcg tcctgctact ttatctacaa accagattgc aaattgctgt
    13621 tggtgaccaa tcgcaaatcc gccaccacca gtcaagcgtt gagttgcctc aacagtcata
    13681 tttgaactaa ctgcccagta aagtccacag atgataccaa tcgctgctgt accgtaagca
    13741 ttgcgcaatt gtggtactaa gaatagaacc ataagagata ctgttgcagc ttgttgtacc
    13801 atgatgtgac cagtaataaa gagggttctt accttcgtaa tctttcgaag agctacgagc
    13861 aagatattta ttccaaaacc aatcaataga gctgtagttg cagttccaac aaaatctgga
    13921 aactctgctg caattttgtt gtttgctgca gcaagtccaa agtaagggtc gataaccgct
    13981 gcaccaattt ggaatttgta gttaagagct gctaagattg gacgaaaggt tgtaaccaaa
    14041 ccaccagcac ccacgttaag caacatatac cctactgttg ctttaacaaa ccctgaaaaa
    14101 acgtcatggg caggtttttt caaaagtgca tatcctatca acaccaataa acctacgaaa
    14161 aatgcgggat tctgcaaaat attgctagaa aaccaattta gaacacttga aacgacttcc
    14221 ataattgttc tccttttgaa ttgtttttgt aagtttatta tataatgcga aagcgcaaac
    14281 aaaaagacca agaattacac agcggactgt tcactcttgg actgtatgat ttatgatgat
    14341 ttatgatgtt ttacaaatta tcttattttc tcgtagctag ataataggga agaattttag
    14401 aataagactt caataagcct tctacgattt cttcttcaga cgaattttct gtcataggaa
    14461 catctacctt cactaaaact tttcgaactt cttgacttct caccttctcg cgtaaagtac
    14521 gacgcttttc ttcattcgcc tcccaccgtt gactttgacc attagagtag gttagataat
    14581 aaatcccttt aacggttgga atgtctaaaa ctttggtctg cttgcccagt gtttgctcat
    14641 ccttcttacg ttcgatgaaa ctgacttcca aagaaattcc aaagtcagta gatgtcccat
    14701 acaaacgtaa ggccatcata ggttctgtca cttgtccgtc tctctgtaga taaacccaaa
    14761 aatgtggtct caaacactgg gcctgattca tccactgact agtctgttgg agttgccatt
    14821 ctggatggct tgcttgaaag gctttggcca gttctgtaaa agcctttcgg gcctcttgtc
    14881 ctttgtggcg gaacgccagc atcttctctc gctctgctcc cgctttatca ggactacggt
    14941 actgcaaacc agaaaagtct agatagtctc ttatcttatt caacatcagt ttaacctcct
    15001 ctaactagca aaaaatcagg tcaaccctga ttttacttat tctgtatcta caacaacata
    15061 gcgatttggc tcatcacctt cagagtaact agtcacgcca tccatacgtg aaataatacg
    15121 atggataatc ttgcgttcgc tatttgacat tggatctgtt ttatgactgc gaccttcttc
    15181 caaaacacga ttcgccaatt tttgcgcata ggtctgcaag acttctgcac ggtgttcgac
    15241 ataatcattg acattgattg taacgtagaa ggttttggaa tagcggttgt aaagataatt
    15301 ttgagccaac agttgcaagg ccttcaagac tttaccatgg tagccgataa tacgacctgg
    15361 ttcgttggtg tcaatttgta gattgatgct acgacggtta taatcatttg aaagtgtagc
    15421 ttcaacatcc atgtcatcca caatcgcttg aacataggca gtcacatccg tagccacctg
    15481 ggcaatatca taactttgct cgaccttcaa gcccatctct ttcaagtctt gattttcagt
    15541 ttgatcttgc tcagtttcaa ggtcatcagc gcctgcttct tccctcatcg cttcctcgat
    15601 ttgaagttca tttaaaatct caatgtgacc agtttcttct aagatagtgc tggcatgtct
    15661 ttcatgtttt aagatttcag ccttgacttc atcagaaata ccttgacctt cttcctctat
    15721 ttttttaata gcattaacca catgaccaag gtcaacggtt tcttcactaa ccgtcttcac
    15781 aggctcgttc aaatcattga tttttttcgg aacgcctttt actacctgtt gatttgcttt
    15841 gacaacagtc gtttcactaa tcgcttcaat atccacttgg gctggttttt taccaaatag
    15901 accaagaaag ccttttttct ccctagaaat gactttgata tgagccttca ttcttggaat
    15961 atctaattct ttcaatcctt tctggattgc ttcttcaaca gttgaacctg taaatactac
    16021 cattaccaga ttcctcctta tttatttcgt tttctgagcc tttttcttgg cttttctttt
    16081 tctattttcc aaatcttttt gtgcctgtac tacggcctcg cgctctgcga taatcttgaa
    16141 tggattattc aagaaatagg tttgcaagac ttgataagca ttagacactg tccagtatag
    16201 ggcgactcca cctggcgcat aaactgcaaa gataaaaatc aagactggaa tcccatacat
    16261 catcgcagtc gtagcgccat ttcgctcaga caaagctttg ttggacaacc aagtacttaa
    16321 aaaggtgaat actgctgcta aaatcggaag aacaagggtt gtatccacac taccaaggtt
    16381 aatccataag aaatgacctg tctttaaaaa gtcaactctt gatagggctt ggaacagggc
    16441 caaaataacc ggcatctgaa tcaaaatcgg ccaaagagag tctgactgtc tgacacccat
    16501 ttctttaaat actttacgca tttcctgctc tagtttggtt ctgctttcca tatctcgacc
    16561 tggatattgt tctcgaagcg ccttaatgcg tggctgagct tcctgcattt tcctagaagc
    16621 caccatttgc acctgaaaga ctggcaagag gactgtacga atcaagaccg taaagagaat
    16681 aatccccact ccgatactaa tatcaaacga taaaaagcga atgatttccg caaagaagta
    16741 aaccaattta ctccaaaaat cagccgattc ggctgtaata tcgctagtta ccccattagt
    16801 cgcacaggct gtcatgatta acagagacag tcctagcaaa ctagtcaact ttagtttctt
    16861 tttcactccc atttccttcc cggtaaatct ttgataattt taatacatgg agtagatttt
    16921 tctccatctc tgcgtatccc aaggtttcga ctccttttcg agcaatgaca acaaagtcga
    16981 catcttctac cagactccct tttgcattct ggataatatg ccgaatccgt cgcttaattt
    17041 gatttctagt gacggcattc cccagttttt tgctaactga tagacctact cgaaaatggt
    17101 ttttctggtt ttctaattgg tagaccacaa atttgcgatt agcaaaactt gtcccctcct
    17161 tgaaaatcgc cttaaaatct ttctctcttt ttacacgaaa gtttttcttc aaaactcaac
    17221 tccatctatt aaattactac tattatacca tatttttcaa aaaagccaat catagcaagg
    17281 ttttggacaa tttcatcaga aaaagaagct ggaaaaataa ctttcccaac tccttgtatt
    17341 gaatattttt ttagtttcac ttattttttc aagcgttcaa cgtcacgggc aatgactaat
    17401 tcttcatctg ttggaataac caagacacgg attttagctg cctctgttga gatgtctcct
    17461 gtaacgccaa agacattctt ttcatcatca acatcacaac caaaccacga aatccctgag
    17521 attacatcac gacggaaact ctctgcattt tcaccgacac ctgctgtgaa aacaatggca
    17581 tctgctccat ttagcactgc aaggtactga ccgatatgtt tttggatacg gtcaacatac
    17641 atttcataag ccaagctagc ctcgtgattc ccttctgcta cagctgcttc tatatcgcgc
    17701 atatcgctag aattagcaga aactcccaaa agacctgatt cacggttaag aacacgactg
    17761 atatcttctg gtgtgttaaa atcctctgta tattgcatta aataaggaat gatagctgga
    17821 tcaatatccc ctgtacgcgt tcccatcata ataccaccaa gaggagtgaa ccccatagaa
    17881 gtgtctacag atttgccggc tttcacagct gtaattgagc ctccgttacc aatatgacag
    17941 gtaattaact tcaagtcttc taatggacgt cccaagagtt ttgcagcttc tcctgctaca
    18001 aactggtgac ttgtaccatg agcaccgtat ttacgaacct tgttttctgt gtaatatttt
    18061 gttggtagag ggtagcgata agctttctct ggcatacttg tgtggaagga agtatcaaaa
    18121 acaactacac tggtaatgtc tggcaacaat tccttgaagg cacgaacacc tgctgcattg
    18181 gccgggttgt gtagaggagc caacaaactc aactcttcaa ctttttctaa aacatctccc
    18241 tcaacaactg ttgattcttt gaaatattct ccaccagcaa caacacgatg tccaacacct
    18301 gtaatctcgt cataagcctt gataatatcg aaacgaatca agtcatccaa taaaatttta
    18361 acggcttgtg tatgattttc gatatccaaa atttgttgtt cagaacggcc gtcaaatttt
    18421 acagttgaaa ttgaatcttt caaaccgata cgttcaatca aacctttggc taacactttt
    18481 tcctctggca ttaagtataa ttgccatttc aaacttgaac ttcctgcatt gattgcaatt
    18541 gtttttgtca taacatgata ccccttttta agcgttttca actattataa caaaatctat
    18601 tttatatttc agtaccttga gtccattttt gaaaattttc tttaaatttc attaaaacac
    18661 ttgcatcttg caagctagca agtggataaa caaaaggctc tactgctatt tcatttttct
    18721 tctgtaagat aaaaatagtc ttagattgtt tggcattagc aaagagattt tcaggtaaac
    18781 taatcatagc aaccagactt gcctcctctt tcaaccagac tttcaacaaa tcactttgag
    18841 gactggtcaa caaatcactc ggagctagaa aaatagcata tccatctgac ttgaggtact
    18901 taaacccttg ttccatgagc aagtgatggg cgtaagtatg ttcttggcta gaagcaactt
    18961 gatgacgcga cgcaacggca tcatcaggat aatagccgac aggcaagtca ctgatgacca
    19021 catcgctttc tttgagcatt tgtggacgaa cggcatctcc ttggacaaag ccagcctgca
    19081 aaccaattac atctgccatg ctagctgcca gatcaatcag taaatcatcc acttccattc
    19141 ccaagtaatc caccttttta tcaagcgagg tcaagaaagt agcgcctaga attcccatcc
    19201 cagaacccat ttcgaggata gtaatttcct cctctttaaa caactcttcc acaataaaca
    19261 ccaaaagcaa agcaatggca tctggtgtaa actggtgatt ggcctgcaag ggttctgttt
    19321 ccccagcctt catcaagaga aactggtagg tcttgagcca ttcttctttg cgtagtgcta
    19381 aacgcttaag ggcctgattg ttgtctttga cctggtttag ctcagtttca ccatccagat
    19441 agatgctgtt ttgctccacc aaggcgtcat aaaagttggt cgccaaatca ctttggatga
    19501 cttggacatt ctctagtaaa taggtatagg cttgttcaat tttttcaaaa tccatattac
    19561 tatcttacca aatttccctt cttttttcca tccttataga aaaatcactc cctgactagg
    19621 cgagtgattt ttgggctact gttaaaaaga attctgagtg atttccttga aacaggtctt
    19681 cagcgctata gagaatctga ctatgcacag atgaaaaacc atgctcaatt agcttgtttt
    19741 ccagttcagc taaatcaaat ccatgatgat tagcttctgt cttggtaaaa tcagcaatga
    19801 tgagtttccc atcttccttc aaatgttgat gaaacagtga gagagccgca tccaaatcag
    19861 gcatatgatg aagaacccga ctaacagcaa ggcaatcaaa ctctttctcc aagggatttt
    19921 tcggtaaatc ttgctccaaa aactggatat tcttgattgc ttgctgctcc actttcaaac
    19981 gagcttgctc caacattttc tcagaaatgt ctacaagagt gactgactta gcctgcttgg
    20041 ctaggggcta ggggcaaggc taatagatcc gtgccaccac cgaaatctaa aatttctttg
    20101 tctgatagaa gatcaatctg tttctcggct gcttgacata ccaagtttgc gaggaagatt
    20161 ttttagggga atcgaaagtt tctgctttgt ggttaaaatc atgtttcata tctttagttt
    20221 agcacaaagt atagtaaact gaaataagat ataaacaaat gaattggagc ttaacatcca
    20281 tttccagcaa ttttctagaa actacagtgg actattttgg attcaacgca ttatagttga
    20341 attgactagg aattctcttt cttttcaggc ttctcttctg attttttctt ctccgctttt
    20401 tcgcttgaat cagtcgctac ctcttccttt ttatctcttt tctcttcttt gattttgact
    20461 gaaggaaaca gaaactcata ttgactctta tccgtacgaa cagtcgtcac caagcttgtt
    20521 ttcttgtttt gatagcttac ctgacctaga ttaaagacct gttccccact ttcttgctcg
    20581 gctttatcct tggttcgttt agccatggca aaagcaacca atttttcttt attcaaagca
    20641 tagtcttgat agtgggcgac ttgtcggttc aagtaaaatt gcaacaaaag actaaagata
    20701 gctgccatgg tgactgcata gaggagaaca cctgccttaa ctttcttctt tttccacacg
    20761 atagatgaac tccctttcta agcctttttg aaactggaaa cgaaaatgaa ccagttgatt
    20821 gtcctctgta atctgagctg atttgaggcc ataaaccata ggctgatacc cccgtccact
    20881 agcatcggtt ttacgaaaat cgtctgactt tgacttaccg atggcgatgt ccttgccatc
    20941 ttgcttcata tagaaacgat tgccttccac tttttcgaac tgcgaacggt ctaattctac
    21001 ctcaagttgg tccacaaaca agagccactc cttttgctcg ctttgttgct ggtagcgaac
    21061 ttctgaaatg aggagctgac tcatagcttg aaaaaggagt aatcccccac tgatgacaat
    21121 gagggcaagc agggattcta acaaggtaaa agccttgacc ttatggctct ttgattgcca
    21181 acaactgttc tgaaccatgg tagacctcca atcctttttc actagaaaat acctgaatct
    21241 caactccgtt gatgcttacc tgattttgcc ccgtctgcag ggccatctta gctaccctca
    21301 agacttcttc cttttgcaag atttttgctt cctcttgcct atttttttga atttgtccca
    21361 aaaggagggt cgcaatgctg gcaaagatag ctagagcgac tactgcttcc agtaaaatca
    21421 ctgccctaat tttttgtttc cttaatgcgt ttaatttttc catttcctag atataattga
    21481 tagcgaatcg ctcctttact ggtctgaaat tcaaccttag ccagggacga attgccccca
    21541 gcacggtcaa atgtaatact ttgacctgat ggggcctgaa ttcctttagg gactggcaac
    21601 ttttgactgc cattgctaag cgtctgccca tctaagttca gactagtctt ttgctgactg
    21661 gctacactgc gtttttgggt ttcccgatag agttcttcaa actccataaa gaaaatctgt
    21721 tcctctactg ccgcaaaagt ggactgaaca gagccggaca agcccaaggc aaggatactc
    21781 acaagactca aagccaagag actttccagc atggtaaagg ccttaatcat tgactttacg
    21841 atttgctact ccatttttat catggtattc tttataagct ttagcctgtt cttccgtgat
    21901 tcgcccatct gcttgtaact tgcttaggct agcatcttca ttcttttcca agctataaag
    21961 ttctgcctgg ctttccacca ccttaacaac agctgctttt cctttgtcat tgactgcctc
    22021 tttttgcttg gtcagattag gtacaaagag caagaaaagc acgctgataa tcagcaagac
    22081 caccaacatc tcaatcaagg atagtaaaac tctcactctt ttctatctca tacatcttta
    22141 ccgaatgcaa cacgagatca cgatacttcc aagtgctgac cagatactca ataacctctt
    22201 ggtcctctat cctgcattcc ataagtaata atagtttgac cgtgtattca ttcttcaaaa
    22261 tcggaacctg ataagtcaca tctacccaat gctcaaaacc taagtctgtc tgctctacat
    22321 ttgctagttc aatatttaaa atcttcattt ttattcctcc tacttatcta ttcgtaaaag
    22381 aagataaaaa gttatgaaaa aatcattact tttttaattc agacagtact ttcagagcaa
    22441 ttttgttaaa acaaaattgg caaccaagcg atttaatact atttgttgac gccaacaagt
    22501 caattttcag acaaacaaaa aaaccgcaag cctgagcctg cggtgaaaga acaatttaga
    22561 aagtttcctt tctatttatt taactgtaat caagccatct ggttatttaa ctgtaatcaa
    22621 gccatctggc tctactgtga actctggctt gtctgccagt gttccgtctg gtttgaggta
    22681 gtaccagcct gttccgtctg cggactggac gaaggcgttt gataccatag cgccttcttt
    22741 agcgtctaag tagtaccatg tgtccttgta cttgacccag cctgtcttca tggcaccttc
    22801 ttcgttgaaa tagtaccact tctcagcaat tttcttccag cctgtagcca tttcgcctga
    22861 gttgtcaaac cagtaccagt tgccgtctgt gtacttcttc cagctgtctg aaagcatata
    22921 gcctgagcca tcgaaataat accaggtacc gttgattttc tcaaacttat cttttggata
    22981 agagccgtct gagtgtacgt accagtagcc agtgccattt ttctgccagc ctgttgcagc
    23041 gctcaagccg ttttcgatgt cttgcttaaa ctgctcacgg ctaatgcccc aacttgcaag
    23101 atatggatat ggatccacat ggtctgagtg gttgtttggt tggttattgg tacagtattc
    23161 atgcgtcttg ataccttcca agtcgtctgt atcaagagtc ttcggcaaac ctgcttcatc
    23221 tgctagattg cgtaagagtt cgatataaag gcggtagtcc gtcatgaact cttctttagt
    23281 tgaatggctt tcaatcagtt caaccgctgc gtaactctca gtattccaac cgcccccaac
    23341 atcccaactt ccgttgttca caggacctac ttgcatgaca cggccatttc cgacaacatg
    23401 tgaaaagaac cctagttcag ggtcctttct atagtggtaa tcagcctcat tttgagctgt
    23461 tgagttacgg ttgcctgttg aatgggcgtg tacttgtcga taaggctgca ccccaacctg
    23521 tggcaagcct gtacgtagtc tgtttctatc gatatccatt ccctatcgtc ctttccatgc
    23581 gtcattcatc tgcttcactg ctgactctac gaaggtgtct aagtctttgt cagtcatgct
    23641 aatattgtat tttgtaagct cagcacggac tttagcgcga gcctgtgcca gtttttcatc
    23701 tcccttgtag ccagtttcag cagctacttg ctcaacggca tttactgcat ttttggccaa
    23761 gatttcaacg attttgatgg tcttttctcc acctttttga accaggtagt ccttgactgc
    23821 cttgactgcg ataccagcca aaataacaag gatgctgatt gctccattag taatgatttc
    23881 agtaatttgt tgcatttgtt attctccttt ttcgatttct tccatgcggt cgttcatgcg
    23941 aaccatttct ttttgaatgt ctccgaccgc gtgagtgatt gtggttaatt ctgtagtggt
    24001 cttttctagg tgagtcatca aacgctcttc tcgtctatta gagtcggcct ttgattgctc
    24061 gtgcaaatcc ataatcttct tctctcgctt gtccgaagtc ttgataagat atcgaatgat
    24121 aataaagaaa agcaagataa acaaaatcgc ccaagctacc tgactttgag cgattttttc
    24181 agcttcttca attggcatac acacctccta ttctttaggt tctcccgttg gatccgtcca
    24241 gtcaggattg ccctctgcat caaatttcat gatatagaat tcatgattca acagaacggc
    24301 gacgttgatt gttgcgattg taccacccca ctggttgaac gcccaaacgg tttcaacatc
    24361 cttgagttgg cgacggccat ttacgatcac aggacgtttt tgaacatcac gatacatata
    24421 gaagtcatcg cttacattct tgcaacgaat gaactctcca ttttctttca tgtagcgcaa
    24481 agcactcgca agatcaaatg gttctgtgat ttttgtaagg tctagtaagt tatctgtgtt
    24541 ttgatttgtt tctgccatgt ctattctcct ttgtctgctg gtttagtttg ttcatcaagc
    24601 agagcttcca gctcatccac tcgtgcttga agtctttgat tcccttccct ttgctcatcc
    24661 aactgaatac tcaagatatt atttgtaatc atcgaatttg ttgaagttgt tgacatttca
    24721 ctaattgtca tttgtaaggc ttggttaagc tgttctgtgt tcattttcta agttctccaa
    24781 tctgtgtgtt cgttttctat tttcaagagc aagctcctga attgctttaa agtgcgatat
    24841 tggtcaacat gttgttattc atgctattgt tttctccatt ttttctattt tttgatttaa
    24901 ttcttgaata gccttgatta agtaaggaac taaagcggta tagtcaatat gcagatagcc
    24961 atctggattc tcaggatctc gtgagacaat tcttggaacg atggtttcag cctcttgagc
    25021 tattagacca atctcctcat gtttcttatt ttcgatgaaa tcaaatgcaa ccattcttaa
    25081 tctgttgatt ttgtccaagg ctttcacagc tgtatctgtg atgttctctt ttaagcgtct
    25141 gtctgatttt tgttccatcc aatacttcac gctaccgcta ccgacctgat tccaccaaac
    25201 aaccgcattc cttccgcctt tgggattcca accatcacca agcacatctt tacttccaag
    25261 ttcgatacca tttgaaaaca caggagaacg agaaaaagta gtattcccat agaagtttgc
    25321 tctcgatgaa ttcgaaaaat ccacttgatc ataaaaaccg acttcattcc tacagtacat
    25381 tttcccatca gtattgacgt tccatgcttt aggtccggca tagttccaat tatttcccca
    25441 gttcgcccag aaggctgtcc ggactccatg cccggcacca ttccccatac caacagagaa
    25501 ctgattgaca cctgaaatcc agcgaccgcc accctggtca aattgaccaa gtgtgaatcc
    25561 accaattcgg ccttgatagg cttctaggaa ggttgagcta gaaatgacgg actcaacctt
    25621 aatagagaag atacgtttag atgtcagtcg gtcaataaaa gcatcttttg cagttaattt
    25681 tctaataagc gcatcgtcaa ctttcaactt ctcagcagtt accgcttcag cttctaatat
    25741 cgtagtcgtg accgaaccag cttcaaaatt ggccgttttg agcttatcaa ccatggcaga
    25801 cttgatgact gctctgtcaa tcagggtctc tccagtgatg tgggtcaatt tcccaactaa
    25861 gcggttatgt ccattggcgc caagattgat tccagagatg atatctccag ccgagttgat
    25921 gttttcaact acccatgagc cagctagttg agtcattttt gtttgcgttg cttcaagctt
    25981 cttattcgca tctgcgactg catcttctgg atgtggttgc catgttctag atttataacc
    26041 tttgtacaag tcaacttctg taatatacaa atcagctgtt cctgatgatg agccattgtt
    26101 atcaaaacga atgtaagcat tatccatttc tccggaatta aaagttactg agacatcttc
    26161 gcatctagag gtagatagtt tcttgctgct aacaactttc ttaacgattg tgaatccatc
    26221 gctctcgcct gctcttcgtc ccaaaatata aacatcatag cttgctagag cactgttgtt
    26281 aaatcctcta aaattcagta catagtcagt atttcgttca agattaaaac ggtgactata
    26341 caaaaagttt tcgtttttag ttgcattact taaacgcata aggtctttct gcccgttgtg
    26401 ataaaagcta tgcttaacca atcttcctaa attttgagtt gagccccatt cattcgtacc
    26461 atttttaaaa tcactattct taatgaggtt agggccgctt acactatatt tcccaacctc
    26521 aacctgaaac agttgattgg tcagagccat gcgagcaacc ttatccgcaa ttccattttc
    26581 agtattgccc aaaatccgct cgtaaagttt actggtttcc ttaacacgct ggaagtcagt
    26641 agtctctact tttcgcacta gttgattggt cacattcgca aattgactat cagcattcgc
    26701 tttgtttgca gaaacctgat cagatattct acccatttgt cgttcagcat tatccttgtt
    26761 tgtagcgacc tgagtcttta aatttgaaat ctgattatct gcgccttgtt tattactgtt
    26821 tatccgattt gaaagatttg aaatctgagt agtggttcct tgctcactgc ttgtaagtct
    26881 atttgaaagg ccactgattt gaccgcccac atcttgctta taagtagtta tctgacttga
    26941 aatatccgtg aacttaccat ctacagattg acgatagcta gcgatttgac tagcgatgtc
    27001 tttattcgca ctagttttaa cagcttcaat cctttgattg atacccttaa catcttcttg
    27061 ataagtagcc ttaccaacga aatcacgatt gaccagctca cggactgctg tcgcttgtct
    27121 cgcgctctcc tcacgagtat agcgctgtag ggcttcctgt cgctgaccgt ctttatttac
    27181 atattcctga atagctgata agtcggttcg caagccctga gctgtccgct caaaggtagc
    27241 cttagcttca gtaatgagac catcagcgtc ctcaggcgca ggactccagt ccgtcgccac
    27301 actaccgatt tcaaccttga ttcctgttac ccaagctgta ccgcttgtag caccttcaag
    27361 attgaatcgc aatgatgtct tcaattgatc aaaatttgtt ttttcagagt agtcataagt
    27421 gaatgtaata tatttccaat ctgccgaacc tttatacata ccaagcgtag cataatctgg
    27481 accactctgt actccggtct cactattttt tctaaaaaga taatgtttga agcaattaaa
    27541 tacattccaa aaatttcgac cttggactac attttcgtac ttgatccaag cgctaaaagt
    27601 aactttttgg tacaaccttg agctgaaatc tggttcaagg ttgaacgtta aagtagagtt
    27661 gttctctagc ctatagcatt ctttttgacc tgtgacgtgg ttttcaggta atttttcaat
    27721 tactgcccca actgtccggg attttatcca tagattccgc ccaccgactt tcatcttcga
    27781 aaattcttca cgaattttcc cagcttcagc cacgacaaga gtcttatctg cctt
//
LOCUS       DAVKLX010000029        25315 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKLX010000029 DAVKLX010000000
VERSION     DAVKLX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25315
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.029"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(231..449)
                     /locus_tag="VW325_001291"
     CDS             complement(231..449)
                     /locus_tag="VW325_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9734697.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(695..973)
                     /locus_tag="VW325_001292"
     CDS             complement(695..973)
                     /locus_tag="VW325_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734698.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVITVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYNIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1187..2818)
                     /gene="rlmD"
                     /locus_tag="VW325_001293"
     CDS             complement(1187..2818)
                     /gene="rlmD"
                     /locus_tag="VW325_001293"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9734699.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGLAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2892..3866)
                     /locus_tag="VW325_001294"
     CDS             complement(2892..3866)
                     /locus_tag="VW325_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9734700.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDSDKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3940..4554)
                     /gene="pmp23"
                     /locus_tag="VW325_001295"
     CDS             complement(3940..4554)
                     /gene="pmp23"
                     /locus_tag="VW325_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9734701.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLTLAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4554..5528)
                     /locus_tag="VW325_001296"
     CDS             complement(4554..5528)
                     /locus_tag="VW325_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9734702.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(5536..6792)
                     /locus_tag="VW325_001297"
     CDS             complement(5536..6792)
                     /locus_tag="VW325_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9734703.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6854..7285)
                     /locus_tag="VW325_001298"
     CDS             complement(6854..7285)
                     /locus_tag="VW325_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9734704.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7287..7889)
                     /locus_tag="VW325_001299"
     CDS             complement(7287..7889)
                     /locus_tag="VW325_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9734705.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7873..8712)
                     /gene="prmC"
                     /locus_tag="VW325_001300"
     CDS             complement(7873..8712)
                     /gene="prmC"
                     /locus_tag="VW325_001300"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9734706.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8712..9791)
                     /gene="prfA"
                     /locus_tag="VW325_001301"
     CDS             complement(8712..9791)
                     /gene="prfA"
                     /locus_tag="VW325_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9734707.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9788..10378)
                     /locus_tag="VW325_001302"
     CDS             complement(9788..10378)
                     /locus_tag="VW325_001302"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9734708.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10501..10683
                     /locus_tag="VW325_001303"
     CDS             10501..10683
                     /locus_tag="VW325_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9734709.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10819..12192
                     /gene="mnmE"
                     /locus_tag="VW325_001304"
     CDS             10819..12192
                     /gene="mnmE"
                     /locus_tag="VW325_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9734710.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIVQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEQLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12239..13174)
                     /gene="dapA"
                     /locus_tag="VW325_001305"
     CDS             complement(12239..13174)
                     /gene="dapA"
                     /locus_tag="VW325_001305"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9734711.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13230..14306)
                     /locus_tag="VW325_001306"
     CDS             complement(13230..14306)
                     /locus_tag="VW325_001306"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9734712.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14892..14964)
                     /locus_tag="VW325_001307"
     tRNA            complement(14892..14964)
                     /locus_tag="VW325_001307"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14929..14931),aa:Thr,seq:ggt)
     gene            complement(15064..15573)
                     /locus_tag="VW325_001308"
     CDS             complement(15064..15573)
                     /locus_tag="VW325_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9734713.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15571..15667)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15696..16127
                     /locus_tag="VW325_001309"
     CDS             15696..16127
                     /locus_tag="VW325_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9734714.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            16209..16586
                     /gene="mscL"
                     /locus_tag="VW325_001310"
     CDS             16209..16586
                     /gene="mscL"
                     /locus_tag="VW325_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9734715.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16628..17722)
                     /gene="hemH"
                     /locus_tag="VW325_001311"
     CDS             complement(16628..17722)
                     /gene="hemH"
                     /locus_tag="VW325_001311"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9734716.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVTLVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17877..19097
                     /gene="pepT"
                     /locus_tag="VW325_001312"
     CDS             17877..19097
                     /gene="pepT"
                     /locus_tag="VW325_001312"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9734717.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSNRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVRSL"
     gene            complement(19140..19769)
                     /locus_tag="VW325_001313"
     CDS             complement(19140..19769)
                     /locus_tag="VW325_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734718.1"
                     /translation="MSKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVAA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENKTTESSTTTGNQEKPVE"
     gene            19734..19955
                     /locus_tag="VW325_001314"
     CDS             19734..19955
                     /locus_tag="VW325_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734719.1"
                     /translation="MDLFQELICLAQKTFYFFLAICRRLLVAIYHVFLKQESYNPRLQ
                     GLTEIRNPDKTMSVQDAIRFAQQRGFNML"
     gene            complement(20032..20679)
                     /locus_tag="VW325_001315"
                     /pseudo
     CDS             complement(20032..20679)
                     /locus_tag="VW325_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521835.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(20783..21343)
                     /locus_tag="VW325_001316"
                     /pseudo
     CDS             complement(20783..21343)
                     /locus_tag="VW325_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(21569..24688)
                     /gene="phtE"
                     /locus_tag="VW325_001317"
     CDS             complement(21569..24688)
                     /gene="phtE"
                     /locus_tag="VW325_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU9734720.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLVGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     SEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSETGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
     gene            complement(24898..>25315)
                     /locus_tag="VW325_001318"
     CDS             complement(24898..>25315)
                     /locus_tag="VW325_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9734721.1"
                     /translation="KNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDETEIPQVENSVINAKIAEAEALLEKVTDSSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
BASE COUNT         7222 a         5228 c         4610 g         8255 t
ORIGIN      
        1 ttgtttgcta atatctcttt cttctgattt tgtttcaagg attattgcta catcattcat
       61 atttttaggt aaataccaac catctggctt atctgaatat cccttgaagc ctaattgatt
      121 aaaagtagtt atttgaccag taccttgatt gatgttttct tcaacttcat taaagcctat
      181 acttctcttg cataatccct aacttgatct tctgtcctta aagtcatttt ctattcctcg
      241 tcactaaaat tatcttcaaa actaataata tcattaggag tgcaagataa agccttacat
      301 attctttcta aattcttaaa tgtaattggt ttatcctttc ccatttgtgc cataacattt
      361 gttgtaagtc ctgccatagc tattacatct gttttcttta gtcctttttt tgctaactgt
      421 atccataatg gtttatagtt aagtgccata atatccctct ttttttcttc attaaattta
      481 tttaattata gcataaatat tgatttcatt caatcttaca ttgatttagt gatttgtttt
      541 agagaatttg ttgtgttatt ccgtccttat tttgccataa attgttttaa gtgggacctc
      601 ttatccttac catttttacc tcctaatttt gagtataaaa aagacgatag agtttttaat
      661 ttctatcgtc tgagatttac atattaaatt ttcatcacaa aataagactc tttaaagaat
      721 ctttaaattc attggtatgc tgcttacaat atccattatt aaaatggata ttgtaagcag
      781 cacaccaact atttattttt tgagttttaa actttggtag aagtcttgaa ctccttatat
      841 ttacataact atttgcctct ataactgtta tgacacgaaa ccaacaagca agttcaagca
      901 cctccatatc tttattcaaa tgaggactaa gatattcaca aactttatca taaacctctt
      961 tatctttttt cataaaaggg gcatacaact tagcaaattt atcctaaaat tttttgttgc
     1021 ctttatcaat catttcaacg ctcctttaaa tataaaaatg atcctatcaa tctttatatt
     1081 gagcctattt tctaattgat accttgcaag aaaggaataa tagctgttcc tataccaagt
     1141 aggaatgtaa taaatgcaga aagtcatata ctgtcattct tttctattaa atcattttga
     1201 agtgtctcaa agcatccatg atggcttctt ctttttcagg tgtacactgt ggaataattt
     1261 gtttatcctt tttagacttg ttgtaatgtt ctcgtaattc tattccacat ttctttttta
     1321 tctgtgcaat atataatgtc gaaactttta attcaaattt attccaaaca tattctttga
     1381 tttgagcata tgttgctttg ctctccgcac ttgtcaaatc catctcatcc agctcaattt
     1441 caacacttat gtgcttatcg acatcgagtt tggacaaaag tgctaccgtc tcgacgtgat
     1501 gcgtttgagg aaatagatcc accggctgga ctttcttcaa ttcatatccc aactcttggt
     1561 atagtttaat atcacgcgcc atggttgcga cattgcagga gatataggcg atgcgatcgg
     1621 ctcctgtttg ggcgcttgct ttgataaagc tttctgtcaa gcccttgcgt ggaggatcaa
     1681 ccaagataac ggttggttga atcccatctt taagccaatt cttcatagca ttttcagctg
     1741 tgtcacagac atagtgggcg tttgaaatat tgttcagctg agcatttttt ttactattct
     1801 caaccgcttc tggaatcagt tcaacaccgt agacttcttt gacatgcttg gcgactgata
     1861 aaccaatggt tccaatacca gaataagcat caataaccac gtcatctttt tttaactctg
     1921 caaagtcaat ggctgtttga tagagtttct ccgccatttc agtattgact tggtaaaagg
     1981 ctaggccagc gatttggaag tcatttccca acatctggtc cgtaatatag tcttgaccat
     2041 aaagagtgcg ccactcctta ccaaaaatcg cattggtatt ctggtcgttg atattttgca
     2101 tgacagacac aatctctggg aactgcttga taacttgttc aatcaattgg tcaacacgaa
     2161 aaacttttgg acgagttgtc accaaaacga ccatgatttg tcctgaatag tgaccacgac
     2221 gcaccacaag attccgaatc aatccagact gttccttttc gtcataaggt tttaaatcaa
     2281 aacgacggag caggtctcga agagctacta cgacttggtc aatgacagga tcctggataa
     2341 agaaatcttc aaggggcatg aggttgtgcg aattcttacg gaaaaatcct gtttccaaga
     2401 caccattcac tcgacgaacg ggcacctgcg ccttattgcg atacttgact ggatgttcca
     2461 taccaagcgt ttcagcaact tctacatctg caattccagc aatcttgtag agactgtcct
     2521 tgacttgctt ggttttaaac ttgagctgtt ctggataaga aaggtgtcct aaatccgcga
     2581 ttcctgaacg caggtaagcc aaatctagat cttgattacg gtgtggtgac tggacaaggt
     2641 atttttcaac ttttccaaag ccaatctttt tattgacctt gaggacacgc atgagaattt
     2701 tttcactcgg taaagcattc tctacaaaaa agaccaaacc atctaccttg gcaactcctg
     2761 ccccttcatg ggtcaaatca acaatttcaa cttctacaat atcatttttc tttaacattc
     2821 ttttctcttt ctattggccg acaaaaaaga cggcaacgtt acggttacca tccattatac
     2881 catgaaattt cttaagaacc aaaactcttg aagaagttga caagggcttg ccagagggag
     2941 cttaagaagt taccaccgtc ctctagcgcc ttatccgaat caaagttaag gttgatattt
     3001 ttaaaactgt cgccagcttg tgatacgatg ctttgtttaa ggtcatttaa ggttttagtg
     3061 aaatctgcat tgctgaggat atcactcttt gagagattca aggcaaaatt gatgatgata
     3121 ttgatctggt ttcctgttat gacctgatca agtttgtaat tttttaaggt atcttcaaca
     3181 atcttgcgga tatcttcttc tgtcagattt cccttgcttt ctttagcttt ggcgagtcct
     3241 gacttgatat cagctagggc aacgtttaat ttattagcat catagcctga tttgtccttg
     3301 ttttcagcat tgatatctga caaagccttt agctcttctt gagccaaatc tttattggct
     3361 tgcggcacct tggctccatt agcttctagc gaatagtaga tcccagccaa agcactctca
     3421 cctgtaactg gaatgggtgc tgctacagtg attttggcgt gttccacacc caaagttacg
     3481 gctgcatttc ggtacatatc ctgagttacc ttagtgatgt tttcaggtgt ttcaatcttg
     3541 acctcaagtg gcgatttgtc acctagcttt tgaatcttgg ctgatgaata caactgtaag
     3601 ctagagtcat tggccacatt catgatttta gaataaacat caggtgtcat ggtcttgagt
     3661 tctttggtat ctgttgaggc attgtagccc agttttttaa gagtttgatt tttttggtct
     3721 tcagataggg aagaacctag gacatattca ggttggacat aggtttcatc gataactttt
     3781 tgaacatctg ttgctgcatg gacgctattc atagctgtta ctgcccacaa gatcgcagcg
     3841 ctagtcagaa agagtttctt tctcataggg aatttcctcc tttacttctt tagagtaata
     3901 tatctatctt aaagaaaact tataacaaaa acacctggtc tagccagatg ttgaaaagag
     3961 agtgaaacat ttgatgatgt aaaggttaag tcgtacctgt ctagaataat aatagtttcc
     4021 tccatttaca tagagttcag caccgtgaaa aatggaaatg gggtgaatat aactataagt
     4081 ctttccagtc ctattaccaa gcaagggggc aacagtctca cgagagtact gtttggctag
     4141 agtcaaggta ttttccttgc cattttgggc gataaaatcg atataggcag gtccaaaatt
     4201 ataggcttga acagctgtcc agatatctac acccttcttc tgcgccagat agagattgcc
     4261 tgtcagagtt tgaatgcctt gccgaatgct agaggcatta tcattgatgg tgttggtgga
     4321 accacttgca gactcactag actgcataac atcgccttct tttccttttg tttcagtata
     4381 aatcatagca agcacaagct cttcgtttgc tggggtgtct ttttcactca atatttctcg
     4441 caccatgggt tgataggtca tgacttgttt gacatcttga tgaacgcggt aagctttata
     4501 gccagcaaaa aggaagactg ctagtacaag cactcttcga attcgtttaa acattattta
     4561 ctttggatat cctcgatatt tttgattaag atagagtagg ttccattttc gttttggata
     4621 aactcaacag actcggcgtc ttgatagacg ttattgggaa cgatgagctc aattccattt
     4681 gataaggaga gtttttggtt ttcaaatttc tttaattggc gactggcatc aatttcatca
     4741 aattgaacag gttctggtac ggcttctttg acttggtcaa taaagctcaa acgagccgtc
     4801 agattgttgt caaaaaggtc attagccaat ttctcaggtg acaattcatt gctttcttct
     4861 aggttgttga aaatagctga tttgaccttg gattgaaatt gaaaatcatc tgtgttaaaa
     4921 gattcagcaa ttctctgggc tgttttttcc agttccttga tagatttttt aggagaaatc
     4981 ttaggagcga cagcaagaag attatctgaa aaatagttca aaaaagtccc gttgtacttg
     5041 attcgttttt caatcaggtg atacttgcga ctctgaagat tgaccaccaa ggcctcgtca
     5101 gcacccgttc caaatccagg caggttattc tgagtcagct tgattggatt atcaacttct
     5161 cctccgaggt gggtcaaggt ctcccgcagg gcaattcgca agaaagcgaa atgttctaca
     5221 ccttctttag aaaattgaac aaaaatcaag tcattggtct tgagattttc agaaatgcta
     5281 aactcctctt tccagagatt agccagcgtt actgatgtct ccaacaaatc gtctgtaata
     5341 tgattgaaga agggattttc ttcttcgaaa atcccagtct tggcttcatc tgaatacaca
     5401 tgttcaattt ttttacgtag gtattcttcg atttttggag taatattgag aaacttatct
     5461 gctaagaaca gctcggtatc atccggactg aactggtgaa taatggcttt cttaatataa
     5521 atgtccataa aagttttatt cctcgtataa tgggaaggca tctgtcaatt ctttgactgc
     5581 acttctcact tcttctaata cagcctcatt ttctgaattc ttaagggttt taatgatgag
     5641 ttcagccact ttgcgacttt cttcttcacc aaatccacgt gcagtaatag ctgctgctcc
     5701 gatacgaatc ccacttgtct tgaatggtga caaggtttcg taagggattg aatttttatt
     5761 tagggtaata ttgacttcat ccagcaagtt ttgagcaact tttccgtttt ctacaacctt
     5821 agtcacatca acaaggaaga gatggttttc agttccacct gaaataatac ggaaatcagg
     5881 gtcttgcaag aagacatctg ccatagcctt gctgttctta attacattgg cagcatattc
     5941 cttgaaggct ggatccaaaa cttctttgaa ggaaactgcc ttagccgcca caacatgctc
     6001 taaaggaccg ccctgaatac ctgggaaaat agctgaattg atttttttag caagttcttc
     6061 gtcattggtc aaaatcaaac caccacgagg tccacgaagg gttttgtggg tcgttgttgt
     6121 tgtgatatga gcgtatggaa ctgggcttgg atgaaggcca gccgcaacca aaccagcgat
     6181 atgggccatg tccaccatga gcttcgcacc gacagcatct gcgatttcac ggaattttga
     6241 aaaatcgata atttgagaat atgctgaagc accagctaca atcagttttg gttttacttc
     6301 ttgggcttgt ttcaagatag catcaaagtc taagagttcc gtttcaggat caacactata
     6361 agaaacaaag ttgtaggttt gaccagagaa gctaacagga gccccatgag tcaaatgacc
     6421 acctgatgcc aaatccattc ccataaccgt atcacctggc tcaatcaagg acatgtaagc
     6481 cgcacagtta gcttggcttc ctgaatgtgg ttggacattg gcaaatttag caccgaaaat
     6541 ttcttttgcg cgttcaatag caagagtctc tacaacgtct actacatcag ttccaccata
     6601 ataacggcgt cctgggtaac cctcggcata tttatttgtc aagatagacc cttgagctgc
     6661 cataacagcc ttggaaacta cgttttccga agcaattaac tcgatattat tttgttggcg
     6721 ttcttcttct ttggcaatag cattccagag atcagcatca tatgctttaa aatcatcttt
     6781 gtcaaaaatc ataggtcttc tcctttattg tgtgactagt ccattagttt gattttacaa
     6841 taagaaaatc aaactaaaag atgcgaataa accgtttctg cattttatca caagtatagc
     6901 caactttttc ataaaatgca tgagcaccca gacgatgatt ggcagaattt aagcggataa
     6961 acccataacc acatcttttt gcttcttctt ccaacccttg tagtaaactt ttaccaatac
     7021 cttgaccttg cgcttgaggt gaaactgcta aagctaagat attaaatcct gctttggaat
     7081 agagtgattc gtaaacttca gcgtggacat atccaagtaa gacatgatta gctgcatcct
     7141 catagccaag taggaaatga tgggaatcct gagacagtct agctagttgg ctagccgttt
     7201 cctctggact aaaagtataa cccaaagcct cttggttgat gtcacatatc gctttcacat
     7261 ctgtttcttg caaatctctt agcatttcaa gcctcctcaa aagaaatctc tggcaaccga
     7321 gcaagaatat cttctcgttt aattgcgcct tggcgtaaga ttttcacctt gtctccagac
     7381 aaatccacaa tagttgaatc ctgtccagtt agaaaagcat cgtcttccag acccagaacc
     7441 tcttggtcaa aatccttcag aatttgttca aaggttacac cacttgcctg acctgagata
     7501 ttggcagacg gcccaatcaa gggacctgtc tctcgaatta aatccagtgt gatagggtga
     7561 ctgggcatcc gaaatccaat agttgcaagg tcagaattta cccaataggg aactcggtca
     7621 ttggcttcga gaataatggt caagggacct ggcaaaaagg tctctacaag tttttgtaga
     7681 taagttggct gattcttaga aaagtgcaag atatcctcga aagaggcgat attgagattg
     7741 agtgccttat ctctaggacg acgtttgagt tggtaaacat ggtcaactgc tttttcatct
     7801 aaggccttgg caaaaagacc ataaacagtc tctgtaggta gaacgacagc tccaccattt
     7861 tccaactctt gtctaatcct gtccatcatc aaccacaacc atcctatttt gaccaaattg
     7921 gtctttgagt gttcgaactt ttttttcagg aagatgtttt ctaaaaagtt cgggaacact
     7981 ttgaccttgc ttgtatccaa tttcaaggta aatcttacca ccatccttga gatagtcttt
     8041 tgcatcttcc gcaattctac agtaaatagc taggccatcc tcgtctgcaa agagagctag
     8101 atgaggctcc gaatgcaaaa cattcaaacc tacctctgac tcatcttcac gagagatata
     8161 gggtggattg gatacaatta tatcatattt ttcagaaatt tctgcaaaac aatcagattt
     8221 tttaaaaaat atattaagat tttgatcgct agcattctct gatgcaagct ctaaggcctc
     8281 ttgagaaaca tctgctgctg tcactgacca atctggtcta ttttttgcta gagcgagagc
     8341 aatagctcca ctacctgttc caatatccag aactgagaga ttcgtctcaa gattttcagt
     8401 tagaataagc tccactaact cctctgtttc tggacgagga atcaaaaccc gttcatccac
     8461 ctttaaatgc attccataaa aatctgcctg tccaatgatg tactgagctg gcttgtgagc
     8521 tgctagttgc tggtaaatat cttctacaaa ttgtttttct tcctctgttg tcacctcctg
     8581 ctggagggca aaaataaagt ctgtaaaaga tagatttttc agactacgat agacaaaaga
     8641 gaggctttcc gcttcctctc cttgtcttat caactcttct tcaaaatttg aaaataattg
     8701 agctaatttc attatttgtt taattcttct agtttttgtg tttggtcata aagcaccaag
     8761 gcatccacaa cttcgtccaa tttaccagac aaaatcgtat ctagtttttg gagggtcaag
     8821 ccgatacggt ggtctgtgac acggttttgt gggaagttat aagttcggat ccgttctgaa
     8881 cggtcaccag taccgattgt cgacttacgc tcagcgtctt gttcatcctg agcaatctga
     8941 gcaaagtggt cagcgacgcg tgcacggata atcttcatag ccttctcgcg gttcttctgc
     9001 tgggtacgtt cttcctgcat ctcaaccttg atattggttg gcaagtgaac gatacgaacg
     9061 gcagtcgcaa ccttattgac gttctgtcca ccagcaccag aggcgtgata gatgtcgaca
     9121 cgaaggtctt ttggatcaat gtcgtattca acctcttcaa cttctggcat aacaagaact
     9181 gtcgctgtcg aagtatgaac acggccttgg ctttctgtca caggaacacg ttgcacacgg
     9241 tgggcacctg attcatactt aagcttagag tatacagact gacctgaaac catagcaacc
     9301 acttctttaa aaccaccgac accattcata gaggcttcca tgacttcaaa gcgccaacct
     9361 tgggcttccg catacttttg gtacatagtt agcaaatctc cagcgaaaag tgccgcttcg
     9421 tctccaccag ctgctccacg gatttcaagg atgatattct tgtcatcgtt tggatccttt
     9481 ggaaggagca aaattttcag tttttcttca tattcttctt tttcagcctt ggcatctttg
     9541 agttcttgct tggccatttc ttccaagtcc gcatctccgc ctgattcctt aatcatctct
     9601 tcggcatcga cgatattttg aaggacttgt ttatactcac ggtaggctat tacggtgtca
     9661 cgattggaag cttcttcttt tgaaagctcc ataaaacgct tggtgtctga aacgacatca
     9721 gggtcactca gcaattctcc tagttcttca taacggtctt ctacagcttg tagttgatca
     9781 tagatgttca tttttcttca ttctccttat tgatttcagg ggcaaaataa tgtttacggc
     9841 aaaccgagat ataggtttca ttaccaccaa tctggatctg ttctccatcg taaacgggca
     9901 gtccatcctg tgttcgcaac accatggtcg cctttttctt gcaatactga cagatggtct
     9961 tgatttcgtc aatcttgtct gctaaaagca agagatattt ggaaccttcg aacaattcat
    10021 tgcgaaagtc atttttcaag ccaaaagcca tgacgggtat gtctaactcg tccacaacac
    10081 gagctaggtc ataaacatgg tggcgtttga gaaactgggc ttcatcgacc aaaacacagt
    10141 aaggtttttc tggtaggtct cggatatagc caaagatatc cgttgtttcc tcaatcgcaa
    10201 gggcagggcg tttcatgcca attcgactcg acacatagcc aacaccgtca cgcgtatcca
    10261 gagccgaggt cataatcaca acaccttttc cttgctcctc gtagttatag gccactttga
    10321 gaatctcaat cgttttacca gagttcatgg tcccataacg atagtacaac tgtgccatgt
    10381 ttcttgcttc acgtccattt ctaaattttt gctacattct agtatatcat aattttctta
    10441 agctttaaac ggcaaaatgt ggtaaaatgg aagaaatcaa aactagtgga ggaagctatt
    10501 atgccatttg tacgcatcga tttatttgaa ggacgcacgc tcgagcaaaa gaaagctctt
    10561 gctaaggaag taacggaagc ggttgttcgc aacactggag cacctcaatc tgctgttcat
    10621 gtcatcatca acgacatgcc agaaggaact tatttcccac aaggggaaat gcgcaccaaa
    10681 taagctagct taagcagaat tgcttaggct ttttcaatct ccaagtagca cccattgaag
    10741 aaatagtcta aatttgttac aatttgaaaa gagacttgga aaattccaag aaaagagcaa
    10801 ttaattaaag gaaacattat gattacacgt gaatttgata ccatcgctgc tatctctact
    10861 ccactaggtg aaggggctat tggtattgtc cgcctgagcg gaacagacag ttttgctatt
    10921 gtgcaaaaga tttttaaagg aaaagacttg aacaaggttg ccagccacac tctcaactac
    10981 ggtcacatta ttgatcctct gactggtaaa gtcatggacg aggttatggt tggggctatg
    11041 aagtctccaa agaccttcac tcgtgaggat attatcgaga ttaacaccca cggtgggatt
    11101 gcggtgacca atgaaattct ccagctagct attcgtgaag gggctcggtt ggcagaacct
    11161 ggtgaattta ccaaacgtgc ttttttaaac ggtcgcgtag acttgacaca ggcagaggct
    11221 gtgatggata tcatccgtgc caagactgac aaggccatga acattgcggt caaacaatta
    11281 gacggctccc tttctgacct cattaacaat acccgtcaag aaatcctcaa tacacttgcc
    11341 caagttgagg tcaatatcga ctatcctgaa tatgatgatg ttgaggaagc tactactgcc
    11401 gttgtccgtg agaagactat ggagtttgag caattgctaa ccaatcttct tagaacagcc
    11461 cgtcgcggca aaatccttcg tgaaggaatt tcaacagcta tcatcggacg tcccaacgtt
    11521 gggaaatcca gccttctcaa caacctcttg cgtgaggaca aggctatcgt aacagatatc
    11581 gctgggacaa cacgagatgt catcgaagag tacgtcaaca tcaatggtgt acctctcaaa
    11641 ttgattgata cagccggtat tcgtgaaaca gatgacatag tggaacaaat cggtgttgag
    11701 cgttcgaaaa aagccctcaa ggaagccgac ttggttctac tagtgctaaa tgccagtgaa
    11761 caactgactg cgcaagacag acaacttctt gaaattagcc aagataccaa tcgcattatt
    11821 ctacttaata aaaccgacct gccagaaacg attgaaactt cgaaactacc tgaagacgtt
    11881 atccgtattt cagtccttaa aaaccaaaac atcgacaaga ttgaagagcg aatcaacaac
    11941 ctcttctttg aaaatgctgg cttggtcgag caagatgcta cttacttgtc aaacgcccgt
    12001 cacatttccc tgattgaaaa agcagttgaa agcctacaag ccgttaatca aggtcttgag
    12061 ctggggatgc cagttgattt gcttcaagtt gacttgactc gtacttggga aatcctcgga
    12121 gaaatcactg gggatgctgc tccagatgaa ctcatcaccc aactctttag ccaattctgt
    12181 ttaggaaaat aagaaaaatc catgatcctt cattcggtca tggattttat tgtctttatt
    12241 agtaatctgg tcttaagacc cctgttacag ttgccttagt tgcttcgtag tcgccatcta
    12301 cgacaacctt gataatgcgt ttggcatctt cttctggtgc tggaacaaga ggtagacgag
    12361 tgggtccagc ttcaaatccc atatagttaa gaattgcctt aactggagca ggacttggat
    12421 aagagaagag agcattaacc ttaggaatga atttacgctg aattgctgcg gctttcttca
    12481 tatcgctttc tgcaatggca gtaaacatct cgtgcatttc atccccattt gtatgagagg
    12541 caacagaaat aaccccatcc gccccaaggt tcatggcatg gaaagcatct ccatcctcac
    12601 ctgtataaat caagaactct tcaggcttgt gctcaatcaa gtaagccata ttagccaagc
    12661 tagtacattc tttgacaccg ataatatttg gatggtcagc caagcgaagc atggtttctg
    12721 gagtcaattc gacaactaca cgccctggaa tgttatagat aataattggt aggtcagaag
    12781 catctgcaat agccttaaag tgctgataca tcccttcttg agaaggtttg ttgtagtaag
    12841 gaacaatagc aagcccagct gcgaaaccac caaattccgc tacttctttg acaaactcaa
    12901 tagagtcacg cgtatcatta gtacctacac ccgcaatcaa aggaacgcgt ccattgacaa
    12961 ccttttgtac agccgcaaac aactccaact cctcatcgtg ggtcaaagtt ggactctcag
    13021 cagtcgttcc tgcgagaaga attccatccg tatgatgggc caataaatgc tcaatcaagg
    13081 ctggaatagc atcaaagtta atggaaccat cctcatggaa gggggtaata aaggctgtaa
    13141 tgattttaca ttcttttaaa tcttgataag acacgaaaac acctctctat ttcaaagaag
    13201 gagttcatct gaactcctaa acgatatgac tattttaatt caaatttcaa ttcagctgtt
    13261 ggacgaacca atccacgttc atgaagagtt tcagcaatct gaactgagtt ccaagcagca
    13321 cctttgagaa ggttatctga aacaacccac atgtgaattc ctttttctgc atccaagtct
    13381 ttacggatac gaccaacaaa ggtatcacgt gaaccaactg cattgatggc ttgaggatag
    13441 atttgatgaa ctacatcatc ttcaagaaca gcacctggga aggatgcgat agctgctttg
    13501 acttcttcaa ttggagccac ttcctttgtt tcaatataaa ctgactcaga gtgagctgac
    13561 aagactggaa tacgcacaca tgtcgcagat actgcaatgc tatcatcttc cataattttc
    13621 ttagtttcct tggtcatctt catctcttcg tacgtgtaat cattatcagt gaagacatca
    13681 atttgtggaa gagcgttaaa ggcgatagga taatgtttct tgtcaccacc tgaaggcaag
    13741 atttccgcat gcaaatcacg tggtttcact ccatcattca agacctcacg aagttcacgt
    13801 tgtgtctcaa gaattgctcc cataccagca cctgaaacgg cttgataagt tgaaacaatg
    13861 atacggtcca agccccattt ttggcgaacc ggctcaagag ccaccatcat ttggattgtt
    13921 gaacagttag ggcaggcaat gattccgttg tgggcatcaa gtgcatgagc attgacctct
    13981 ggaacaacca aaggaacatc tggattttga cggaaataag atgtattatc cactactacc
    14041 acgccagctt ttactgcgta tggtgcatac ttagctgatg tcgaaccacc tgctgaaaag
    14101 agagcaatat caactccttc aaaagctgtt tcagtcgttt cttcaatcgt aatatcttga
    14161 tctttaaatt tcaatgactt acctgctgaa cgtgcagaag caaggtaacg gattttatcg
    14221 attggaagtg ttgattcttc caacattttt atcatctgag caccgacagc acctgtcgcg
    14281 ccgactacag caactgtata tcccataaat aacctctttc ggaattttct aaaaatttct
    14341 ataatagaag tattatacta ctttttccat gaattgcaaa gctttttcat cattttttgg
    14401 aaaataaaaa tccccaatcg ctagactagg gattaagagg catcttcatg tgatgagcag
    14461 gttcacacaa ctcatcaagg tccgctcctg cgttatgacc tccttatgct caatagtagc
    14521 ccgaagacta cctatcgact catcgcatct actattctac taaagagaat gacttttgtc
    14581 aacagacgaa actctttata ctcttcgaaa atctcttcaa acttcgtcag cgtcgccttg
    14641 ccgtatgtat ggttactgac ttcgtcagtt tcatctacaa cctcaaaaac atgttttaag
    14701 ctgacttcgt cagttctatc tacaacctca aagcagtgct ttgagcaacc tgcggctagt
    14761 ttcctagttt actctttgat tttcattgag tattagtttt atttacacag gtgcataaga
    14821 aatatattgg aaaaattggg ggcagacata acaaaaaccc ttgtaaatca agggcttttc
    14881 tgtctattta atgctaattg ccgggattga accggcgacc tcatccttac catggatgcg
    14941 ctctgccaac tgagctaaat cagcttactt aagaagtata ctatattcag aaaaacttgt
    15001 caagtttcct tagaaatttt ccataggaaa aagccaggta agaactacct agcttatctt
    15061 cttctattta cttaaatcaa ttcgacgacc aattttacca ataataatcg ctccaataat
    15121 caatgaagca aattcaccga ttcctgttgt aaaccaagta aggaagaatg gtaattctgc
    15181 tacaatattt aactctgcag cgatagtaaa catggaaatt gaaaagagga ttgaaaagaa
    15241 gaaatgatct tttcgaatca atccgttaaa gaggtaatcc ttgctatatt ttgcaaaaag
    15301 ccaaacacct aaactgagga atactaaggt tgaaccacca ccaacaaaga catctagcag
    15361 tccgaagcta aagaaattag caatcataca accgatggta actccgatga tgtacttagg
    15421 attgtaaaaa gccataaagt tcatcatttc tgaaatacga aactgataag caccatagct
    15481 gatggcattt aggggcggtg tgacagtcaa aacgacgtag atagcagcga cgattgcaat
    15541 atctgcaaca tcacgaatag ttaatttttt catcttttct cctttggcgg ttatccgcgt
    15601 gtaatatgct tggtgaaaga agctaagcac caagggttga ttcattcaac cttactagta
    15661 tagcacaata gcctgcttta tgctatactt aaagcatgaa aaaacctatt caaaaatttt
    15721 ttaacaatga aatcttagcc tatctctttt ttggccttgc gacaactcta gtttcgattc
    15781 tctcacgact agtcatttat caactaagtc atcaggaact ccttgctacg gccttggcaa
    15841 atattatcgg tatcttattt gcctttatca caaatgatac gattgtattt aagcaaaaaa
    15901 agaaaaatag acttatccgt ttagtgaaat tttctcttgc tcgcctttct acttttctgt
    15961 tagacttgtt tttaactttt ctctttgtaa cccaatttcc tcacatcata gggcaattcg
    16021 taactgaaaa cattgataag ataaatgcta ttgaaacaat aattgcacaa atattcatta
    16081 ttatcatcaa ttatatcttg agcaaggtct atatttttag aaaataatgc tccatttatg
    16141 agccttttaa ttgccttcta taatgattta ggatacaatg aaacacgaaa cattgaaagg
    16201 aaaacagtat gttaaaaaat ctaaaatcgt tcttgcttcg aggaaatgtt attgaccttg
    16261 ctgtcggtgt tgtcattgcc tctgcttttg gtgctatcgt tacttcactt gtaaacgaca
    16321 ttatcactcc tcttatttta aatcccgctt tgaaagctgc taaagtcgaa cgtatcgctc
    16381 aactttcttg gcatggagtc ggctatggta actttttaag tgctattatc aattttatct
    16441 ttgtgggtac cgccctcttc tttattatca agggcattga aaaagcacag aaactgactg
    16501 gcataaagaa agaaaaaact gccgaaaaaa aaccaaccga attggaagtc cttcaagaaa
    16561 taaaagctct ccttgagaaa aaataatcga ttaaaaggat ctagcgcaaa gctaaatcct
    16621 tttttcttta ctctttgggt aacttgcctg ctttttcaag catttttttg ataagataag
    16681 gcatcttaac attctcccgc ccttttttct caatcagttt tttcacaaat tctggcattt
    16741 gtaaatcttc agattcagct aaaatgtttt tcgtctcgtc tgaaggaact aactcatcaa
    16801 aggtctcttc ttctgggaga aattccttaa actcttgatt ttcattgact ctaactgtat
    16861 tgaccaaagc atcaatcaaa cgagaatctg tatttggcat tggtggacga tggtagttta
    16921 ccccaaattc ctggcataag tcataacatt ctacatcatt gtcaaacaag acctcaatat
    16981 gctcactgat gaaactaata ggtacaaaaa tataatggtc tggatgttct gtctgttctc
    17041 tgagatactc caaaacatct ggtttaatcc atggaatacc aatatcactc tcactctgcc
    17101 aagtgttggt atattgttct gaactcaagc ccagtttttc agcgactagc ttgctatttt
    17161 cgaaaatttg gtctatataa ggatcaccaa agtccaaggc aaaaatgggc acactatggg
    17221 ctgaaaagat gactttaaag ctgtcttgct ttacttcttc ttttaaaatc ttagcaattt
    17281 catctgccca atagtttaag agcgcttctt cctgatacca gtccttaatg accaaaaatt
    17341 gaatttgttt gctttctaaa aatttttcat agcccatgac agagtagaaa gaataatggg
    17401 gctccaaaat caagcaaata cactgttcaa ttccgtctgc ttccatttga ccgatcacat
    17461 ctggaataaa gggactggaa aacttattag caaagtagac actgtattca ttccccagcc
    17521 tagcttctac caaggtaacc tcttgacggg taattttttg cagaggcgtg cctccaattc
    17581 gtacataatt atcatagaga gtctgaatct cgtggtcttg gggtctcacc ccacgacgaa
    17641 tatttgtgaa aaaatcagct acaccttcaa aggtaatctc ttctggtgaa ccgaaggtca
    17701 tcattaaaat tgcttttttc atgactgctt ccttgtgatg tttttatcaa gtttatttta
    17761 tcattaattc atcgataagt aaacgctaac atagggacat tctcaatctt ctgtcttttg
    17821 tttttctctt ctttctatga tacaatggaa aaaataaatt caaaaggagt ttttttatga
    17881 cttatccaaa tctcttggac cgcttcttaa cctacgttaa ggtcaacacg cgctctgatg
    17941 aacactctac tactactcca agtacacaga gtcaggttga cttcgcaaca aatgtcctaa
    18001 ttcctgaaat gaaacgtgtt ggactgcaaa atgtttacta tctaccgaat ggttttgcta
    18061 ttggaacctt gccagccaac gatccgtctt taacacgtaa gattggtttt atatcgcaca
    18121 tggatactgc tgattttaat gctgaaggag tcaatccaca ggtaattgaa aactacgatg
    18181 gtggtgtgat tgaactaggg aattctggtt tcaaactcga tccagctgac ttcaagagtc
    18241 ttgaaaaata tccaggacaa acgctcatca caacagatgg aacaaccttg ctaggtgctg
    18301 atgacaagtc aggaattgct gaaattatga cagccattga atatctaact gctcatcctg
    18361 aaattaagca ctgtgagatt cgtgttggtt ttggtccaga tgaagaaatc ggtgttggtg
    18421 ccaataaatt tgatgcagaa gattttgatg tggattttgc ctacactgtt gatggtggtc
    18481 cactaggtga acttcagtac gagactttct cagccgctgg tgctgaattg catttccaag
    18541 gccgtaatgt ccaccctggt actgccaaag ggcagatggt caatgccctt cagctagcaa
    18601 ttgattttca taatcaactt ccagaaaatg accgacctga gttaactgaa ggttaccaag
    18661 gtttttacca tctaatggat gtgacaggta gtgttgagga ggcgcgtgca agctacatca
    18721 ttcgtgattt tgaaaaagat gcctttgaag cgcgtaaagc atccatgcaa tctatcgctg
    18781 ataagatgaa tgaagaactt gggagcaacc gtgtcactct caacttgaca gaccagtact
    18841 acaatatgaa agaagtcatt gaaaaagata tgactccaat taccattgct aaagccgtta
    18901 tggaagatct aggtatcacg cctattatcg aaccaatccg aggtggaaca gacggctcta
    18961 agatttcctt tatgggaatc ccaactccga atatctttgc aggtggcgaa aatatgcacg
    19021 gacgttttga atacgttagc cttcagacta tggaacgtgc agttgatacc atcattggca
    19081 tcgtacgtag cttataaaag ctaaaaagac gaggtagctc agctacttcg cctttctttt
    19141 tattctactg gtttttcttg atttccagta gttgtagaag attctgttgt tttattttct
    19201 gaagttgatt cagcaggttt agaatctctt gtattgcttg gtttgttttc gtcgctagca
    19261 gtttcaatgt tagattctgc agttgcgttt ggttggttct cagcactggt gttatcacca
    19321 tttgcttcag catttcttgc tggacttgtt tcttcacttg cgctagcttt tgactggatt
    19381 tgatgattca aaactagaat agcttttgtc gattcaagta aagctgtttt gtctttactc
    19441 ttagcagaaa gttgatctaa taatgcagcc accttatcaa agtccgcatc agatccatta
    19501 ttactttcta aataagagtg aagcgacatg agaatatcgt agagtttttg atagagtaca
    19561 agtgtctgag gatcttgctc agcattttcc ttttcttgtt gaagggcgct agcgatacga
    19621 gtcaagacat cttttacctg actgtttact tcatccaagt ctgcatcagc cttgtttgtg
    19681 gcagctttta gattttctac ttcttctgcc aaggattgtc tgattccttc ttcatggatt
    19741 tgttccaaga gttgatttgc cttgctcaaa agactttcta cttcttcctt gctatctgtc
    19801 gcagattatt ggttgctatc taccatgtat tcctaaaaca ggagagttat aatccaagat
    19861 tacaaggcct tacagaaata agaaatccag ataagacaat gtccgtccaa gacgctattc
    19921 gcttcgcaca gcagcgcgga ttcaatatgc tttaatttta aagtttaggt gtcaagacct
    19981 ctttttagtg tgcccaaaat ttagagaagt aatcaatcaa ctaactttta tttttttcaa
    20041 actttcagta aactgaccta aagctaactc aatctgtctt tgttcgatag gcttgtcttt
    20101 gtagatgctt ctgctatcag ctagaagttg atctactttt gccaagactg ccttctcatc
    20161 aaaagttcca ggtggatagt tggattgcag ggatggaatc ttgtttttca aagccgcttc
    20221 atatccctta gtttgaacct tgatgtagtg attgtggtcg ccatgaggaa tcacaaaacc
    20281 ttctgaatct tcacttataa ttcgattggc atcaaaacca tgaccatctt cttcctcatg
    20341 gtggacatgt agtgacggat tacttaatac agaactagaa gaacttccta cctcttccgt
    20401 gttagagtgt gatgggggat tgttaagaga tgacttagga atatagtgat agtgatcccc
    20461 atgtcttact atataagcat cacctgtatc tctgacaata tcattagggt taaagacata
    20521 accatcatct actgcagaaa caccattatt cggtgtcacc ggcaaagatt gactgagagc
    20581 tgtagtattc cctgataatt atacttttgc gactgctaat tcacctgccg acaagtcact
    20641 ctcaggaatg aaatgatagt gaccaccatg tggtactatc gtagattgaa atagaatatg
    20701 agcaaattga taaggggatt ttaaagtaat ttctaacaat gatttagaaa ctatgatgtg
    20761 ctattctaaa ttcaactcac tatatataac catcatcggt agtataacgt ccctgtaatt
    20821 ttgctacaga tacttctgca ctagctcctt tatcgtcttt accatgttct tgtttttggc
    20881 gattgatttc atcttttgtt cgtacatttt ctgcatgagc ttgatcttta aggtaaacat
    20941 aatactttcc atctccctta ataatatatc ctcccttaac ctaactgacg atatcttgat
    21001 ctttcggctg atagttgggg gctttcatta gtagctcttc actaaagagc gcatcaaaag
    21061 gaactttacc attatagtag tgataatgat cgccatgaga agttacataa ccttgatctg
    21121 taatcttaat aacaatttgt tttgcttgaa ttccttcttt ttgactaacc tagtctggag
    21181 tcaaattttc agtcttctta gtgtctttat tactgtttac atatgaaaca cgatttttat
    21241 ctgtattggc ctgttagcta tgttggttca gagcataaac acacagactt aaggaaagga
    21301 taacaacaga tccagctgct atatatttct ttttaaattt cataattacc tcatttctat
    21361 aattatttat atgatgtctt cattattaaa tgattaaata aattaattaa ccagttaatt
    21421 aattagtaaa tattccacct ctttttaagt tgtatgtcaa gaaattttat atattaataa
    21481 taaaatgaaa ttctcccaaa gtcagagttt tatttctaac ttttgagaga acttcatttt
    21541 tgattcagac tttttctact gctattcctt acgctatgag atcagataaa ttctttttta
    21601 tcacttctcc acttggcaat cttaattcaa tcgttccatc catattgaat ataacactat
    21661 ctaagcctaa tccgtaacta gctgtaaatt tttctaattt ttcttgtaca ggatctactg
    21721 ctggagcttc ctctaatgct ggatctaaca tagggtcact ccccacattc ccttctggat
    21781 tcaacattcc attatccgtt gagttttctg gttttacagg tttttcgttt ggtgcctctg
    21841 gtaaagaatc tgctggttta ttttctgttg gttggttctc aactgttcca gtagatactt
    21901 ttccagtttc agatggttta ttttcaccat ttccttgagg tgcttctcct gtaaaatctg
    21961 ccatattctt tttaatgact tctcccgatg gtaaatataa ttcaattgtt ccgtccatat
    22021 taaacaagac attttctagc ttcatcccat aactttcagc aaattttgct actttttctt
    22081 gtacaggatc cactgtagga acttcttcta acgttgaatt actagtacta ttcccagttt
    22141 cagaaagttt ttctttttct accttctcac tagtctttgg ttcttctacc ttttcatcaa
    22201 gttttgagtt ttcttgtgct ttattccttt taaattgtgg tagaatactt ggtttatcag
    22261 tttgattttc tttttccaag ataggtactt ccacaatata agtcgattga ttgtccaaat
    22321 aagcatttgc catgaaggtt acaggaattt tatttccggc cgttctggtt gttccttggt
    22381 ttaatttcgg aatcggtaat ttgatttcac caactttata gttattttct aaataagcat
    22441 ttccatgaaa ttcatcaaac actctgacta aagcatcagt tcctttaggc actgcaaatt
    22501 gagggttcac tcttaaataa gtatcccctg catggaaagg atagaaaatc gtttgactgg
    22561 ccattttgta agctaaagag gttggaactg taaatgtacc atcataactt acttctggat
    22621 aatcttttga agcgatagta tacttaaatg tttgtcctgg taaataaggc tgatctaatt
    22681 caaagtttgc aatattccct actccttctc caaatacttt accagatact ttctccaata
    22741 cttttccatc tggtgttatt aattttacta gcatattgat acctaatttt ttctccaatt
    22801 caggcggaaa actaaaagaa acgcgttttt gaccattggc tagagtaaag ttttgattat
    22861 taaacgtact attttttaac aaattaacaa cattcgttaa ttcttctcca gtataaactt
    22921 tattcccttc ttttttagca actccttctt cgggtttaaa cagttcatag ttactgtgag
    22981 aatgaccaat tccaaccggt ttatgttcat caatcggatc tgcatgatgg tgatctccat
    23041 gcggataaat aatcgcattt ttttctttat tcacgacaat actttcacgt ttgacaccat
    23101 attgtttcat aatgccagca attttttctt caattttttt atctaaatct ttcatttctt
    23161 tggcattact tggataatcc tgttcatgag atgacaaaga atctaatcca ttatgactag
    23221 ttttaacttc ctctaaatgt ttttgcgcag ccttaatttg ctcttctgtc aagtccttct
    23281 tgaagaaata atgattgtgg tctccgtgac tcatgacaaa acctgattca tcttcagaga
    23341 taatacgatt agcatcaaat ccgtatccat cttcttcatg tttctcatgt gaagttcctg
    23401 gattgattgg aagagatgga gaaggtgttg ctagactatt gtttggaaga gtcggttgcc
    23461 caatttgatt tgattttgga atgtaatgga aatgatcacc atgtcttaca atataagctg
    23521 tagccgtttc ttcaacgata tcttttggat taaaaatata accatcagat gctgaagaga
    23581 gctccttact tgtcgttaaa gaagaaggat tgcttgaaag actgcctaga ctagacacta
    23641 cttcattagg ttttgcattt gtagaaactg tagaaccagt tccactgata ggcaccattc
    23701 tggcaatctt ttcttctaag gcagaaagct tgctgtaagg aataaagtgg taatggtcgc
    23761 catgcggaat cgcaactcca tttggtgtac gactgataat cttagcaggg tcaaagacca
    23821 ggccatctga ttcactgtaa cgttgggcgc taggtgaatc atagagttcc ttcaaaagac
    23881 tctggagatt ttcagattta tttgctggct tgctagttga tccttttgct acagattgcg
    23941 tgttattgtc actagctgtt gaagaatagc ttaactgact cggttgcata ttttttccaa
    24001 ccagatgtgc tttagctgct gctaattcac tagcagataa atcgcttttg ggaatgtagt
    24061 gatagtgacc tccatgagga acgatataag cattacccgt atcttcgata atatcagctg
    24121 gattaaagac ataaccatca tttgtcgtat atcgtccctg agaccttgct acagcaacat
    24181 tagagttaac cttctcatta tctttgacat gttcttgttt ttgacgattg atttcatctt
    24241 tagttcgaac attatcagca tgagctgcat ctttcaggta gacataatat tttccatcga
    24301 ccttgatgat ataaccaccc ttgacttcat tgacaatatc agcgtcttta agttgatagt
    24361 ttggatcctt catcaagagt tcttcactaa agagggcatc ataaggaact ttcccattat
    24421 agtaatgata gtggtcaccg tgtgacgtta catagccctg atctgtaatt ttgattacaa
    24481 tttgctcagc ctgaattcct tctttctggc taacctggtc tggtgtcaag ttttcacttt
    24541 tctgacttga ctggctgcca tccacataag agacacgatt attgtcctta ttttcctgcg
    24601 aacgatgctg gtttagtgca taggcacata gactcaagga tacgataaca gctgatccag
    24661 ctgctatata ttttttacta aatttcataa atccctcatt tcaataaatg atgaagtttt
    24721 ttctcaactt cttttacttt attaaatagt tttctaaacc ggttatttaa ccatttaatt
    24781 aaccagtaaa tagtttacta tttaaaatta cttttgtcaa gtttctgtta cgtttttcta
    24841 aaaaagagaa gtaacacgaa aggttacttc tccataaaat atgctccatt cattttacta
    24901 ctgtatcgga gccggttgac ttttttttaa caaagccaag agactatcta cttctgctga
    24961 aatagtgtta ttatctttcg ttccgagaag aagactactt tttagaccag tcaatgtctc
    25021 catagcattt tgtctaatac tagaatctgt tactttttct agcaaggcct ccgcctctgc
    25081 tatcttagcg ttaataacag aattctctac ttgaggaatt tcagtctcat ctgtagtatc
    25141 ttcagcttct tcctctgtct cttccgtatc agtgcttggt ttataaagat tatctgctga
    25201 aggatttaaa ccagcgtgat tctctttttc atcagattca gggtgagttg gctcacttac
    25261 ttcatcatgt cccttatctt catcaggttt actgtcttgg tctgccttat tttta
//
LOCUS       DAVKLX010000030        24649 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKLX010000030 DAVKLX010000000
VERSION     DAVKLX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24649
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.030"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            436..1146
                     /gene="deoD"
                     /locus_tag="VW325_001319"
     CDS             436..1146
                     /gene="deoD"
                     /locus_tag="VW325_001319"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9734722.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVEYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1169..1693)
                     /locus_tag="VW325_001320"
     CDS             complement(1169..1693)
                     /locus_tag="VW325_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9734723.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRK
                     LDQFLDKKRKSYNS"
     gene            complement(1746..1982)
                     /locus_tag="VW325_001321"
     CDS             complement(1746..1982)
                     /locus_tag="VW325_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9734724.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2050..2970)
                     /locus_tag="VW325_001322"
     CDS             complement(2050..2970)
                     /locus_tag="VW325_001322"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9734725.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEIYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3160..3565
                     /locus_tag="VW325_001323"
                     /pseudo
     CDS             3160..3565
                     /locus_tag="VW325_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3781..4362
                     /locus_tag="VW325_001324"
     CDS             3781..4362
                     /locus_tag="VW325_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9734726.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4359..5636
                     /locus_tag="VW325_001325"
     CDS             4359..5636
                     /locus_tag="VW325_001325"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9734727.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYCPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5654..6316
                     /gene="deoC"
                     /locus_tag="VW325_001326"
     CDS             5654..6316
                     /gene="deoC"
                     /locus_tag="VW325_001326"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9734728.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGSDFVKTSTGFSTGG
                     ATIADVRLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6303..6692
                     /locus_tag="VW325_001327"
     CDS             6303..6692
                     /locus_tag="VW325_001327"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9734729.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6782..7834
                     /locus_tag="VW325_001328"
     CDS             6782..7834
                     /locus_tag="VW325_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9734730.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7978..9513
                     /locus_tag="VW325_001329"
     CDS             7978..9513
                     /locus_tag="VW325_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734731.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVVDISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            9506..10564
                     /locus_tag="VW325_001330"
     CDS             9506..10564
                     /locus_tag="VW325_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734732.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10567..11523
                     /locus_tag="VW325_001331"
     CDS             10567..11523
                     /locus_tag="VW325_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734733.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            11608..12724
                     /locus_tag="VW325_001332"
                     /pseudo
     CDS             11608..12724
                     /locus_tag="VW325_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(12767..13408)
                     /gene="plsY"
                     /locus_tag="VW325_001333"
     CDS             complement(12767..13408)
                     /gene="plsY"
                     /locus_tag="VW325_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9734734.1"
                     /translation="MITIILLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            13544..15487
                     /gene="parE"
                     /locus_tag="VW325_001334"
     CDS             13544..15487
                     /gene="parE"
                     /locus_tag="VW325_001334"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9734735.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KVGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRSDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            15907..18378
                     /gene="parC"
                     /locus_tag="VW325_001335"
     CDS             15907..18378
                     /gene="parC"
                     /locus_tag="VW325_001335"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9734736.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            18510..19532
                     /locus_tag="VW325_001336"
     CDS             18510..19532
                     /locus_tag="VW325_001336"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9734737.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            19588..20292
                     /locus_tag="VW325_001337"
     CDS             19588..20292
                     /locus_tag="VW325_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9734738.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            20357..21046
                     /locus_tag="VW325_001338"
     CDS             20357..21046
                     /locus_tag="VW325_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9734739.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            21043..21966
                     /locus_tag="VW325_001339"
     CDS             21043..21966
                     /locus_tag="VW325_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9734740.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            21981..22625
                     /gene="pcp"
                     /locus_tag="VW325_001340"
     CDS             21981..22625
                     /gene="pcp"
                     /locus_tag="VW325_001340"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9734741.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            22793..23023
                     /locus_tag="VW325_001341"
     CDS             22793..23023
                     /locus_tag="VW325_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9734742.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            23073..23153
                     /locus_tag="VW325_001342"
     tRNA            23073..23153
                     /locus_tag="VW325_001342"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23107..23109,aa:Tyr,seq:gta)
     gene            23163..23234
                     /locus_tag="VW325_001343"
     tRNA            23163..23234
                     /locus_tag="VW325_001343"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23195..23197,aa:Gln,seq:ttg)
     gene            23396..24598
                     /gene="rpsA"
                     /locus_tag="VW325_001344"
     CDS             23396..24598
                     /gene="rpsA"
                     /locus_tag="VW325_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9734743.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
BASE COUNT         7409 a         4346 c         5602 g         7292 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaccccacc
      181 ctaaaagcag tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc
      241 tattttccct tcgctttttg atgggtttgg tatctttctc aatataaaat ataaaataaa
      301 gaaaggtaga gcgtgtgttt tgatttgaac acgagcggaa aactcggaaa atagataatc
      361 tgactgaaaa atcaggattt ctcgtcaggt tcctaatttt cagtcgtttt cttctcgctc
      421 tttgtatcat aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa
      481 attcttcttc ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttggtgat
      541 gctgtttgtt ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac
      601 cgtgtatctg tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag
      661 ttaatcgtag aatacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat
      721 gaagaggttc atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc
      781 gttcgtaatg actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat
      841 aaagcctacc atatcgccaa agaacttggt atgactactc acgttgggaa cgttttgtca
      901 tctgatgtct tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc
      961 aaggctgtgg aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat
     1021 gcgctagcta tcatgaccat ctctgatagc ttggtcaatc cagacgaaga cacaactgca
     1081 gaagaacgtc aaaatacctt cactgatatg atgaaggttg gtttggaaac cttgattgca
     1141 gaataattat agccaaaaag gaagcccttt atgaattgta ggacttcctt ttcttatcca
     1201 gaaattgatc tagctttctc tgatttcgaa gaatagtgac tttatgtgaa tattcttggc
     1261 aaagtttttg gtaattttct ttttgagttt tgctacgccc atcccaaaga atccatctga
     1321 taaactccca ctcaaagcgt tcagggcaat ctgccgccat actttctctg acttttccac
     1381 ggtatttaag ataacgctta aaggctctaa agagacaggt taatggcgaa aaattgagaa
     1441 agatgatttg gtcagcttct tgcattcgtt cttggtagta gcaccaagaa taattaccat
     1501 cgatgaccca agctttatgc ttggtgagaa agttttttat ctcggttaac atccattcgc
     1561 agtcactgtc ttgccaacca ggttgaaatt ggagtgtgtc catgtgcagt tttggaatgg
     1621 agtagtagtt agataacttt tctgctagag ttgacttacc agaaccagaa tatccgataa
     1681 ttgcgatttt cattttctac cttttcctat ttggagacaa aaaaacagcc tctatggact
     1741 gtttcttatt tagcaagttt agctgaaaga cgagctttat cgcggcttgc tttgtttttg
     1801 tgaatcaaac ctttagtttc tgctttatcg atagctgagc tagcagcacg gaaaagttct
     1861 tcagatgggt ttgcttcgaa agcttttata gcagtacgca tagctgattt ttgagctgag
     1921 ttcttttcgt tttgtttaac gttcaattca gcgcgtttga tagctgattt aatgtttgcc
     1981 aatgttctta cctccatatt tactaactat accattatat atgaaaactt acgttttgac
     2041 aagggaaaat tactttttta agtaaatttc atcgatttca tggttcttgc ttttatgaag
     2101 aatcacttct gcacgatttc tggttggttc aatataattt tgcagatttg tgagattgat
     2161 actgatccag acctgatggg caaaggactc tacttcccca atcggcatct gagtaaaacg
     2221 ataatagtag ctatcagggt cgttttgggc taggctcagc attttcaaga aacggtccag
     2281 ataccaactt tcaatatcat ccactccagc atctacatag atggaaaagt caaagaagtc
     2341 agtgatatag agacgatcgt tttgtggatt ttgaaagaca ttgattccct caacaattac
     2401 aaaatcagca gctttgacac tttgtttctc ttcgggtacg atgtcgtaaa tttcatgaga
     2461 atagacagga atatctacat cttgtccatt tttgatgcgg tccaagaagt tgagaagagc
     2521 ttccatatca tagctttcag gaaatccttt acgatttaaa atcccctgct caatcaaggt
     2581 ttgattggga tagagaaaac catcagttgt aaccaactca accgtagcat ctgtaaacgt
     2641 acgggacagt aggatttgaa gtaggcgact ggttgtggat tttccaacgg caacactccc
     2701 agaaacccca ataataaaag gttgagattt actttcacgt tggaggaaaa ttccttttga
     2761 aaaggctaaa tcttccttag ttcgcttgta aatctgaatc agatgagcca aggggagata
     2821 gatatctgta acgtcttgga gactgatttg gtcattaaaa ctcttgatag attccaattc
     2881 ttcttctgtt aaaggaggtg ttgtttttcg atgtaaagat tgccaagtct ggcggctgat
     2941 tttttcaaaa tgtaaaaatt cgttggtcat ttgttttccc ctagatattt tgtctatcat
     3001 accataaaaa gctaaaaaaa taaagcggtt tcttgaatgt ggtaagtcaa tggtatataa
     3061 tataagtgat caatttattg atgaaatagg aggtagattc tactgattta taagcttata
     3121 aagggggctt tttagttcac aacttatagg atgtgtacta tgaaaatctt aaaatgttac
     3181 atattggaac tctgttttat tttaagtttt gctttacctt ttataaaagg agccaatgca
     3241 gataatggta gatgctttgt ggaaacctat tatggtttta cttttttgat ggaacatgct
     3301 attgtaacag ctgtctttat ctgttcgctc ttaattgctt tcttactaaa aaaaacgatg
     3361 ggcgaaatgg attgctgctg gtagttattg ctttttagtc ctatggattg cgacagaggg
     3421 ttatttcttc cacatgtcac tagaagattt gatacgactt tggacaagtt tggaaatcct
     3481 gacacaaacg tatcagttgg gcttttatct aaatattttg ttaggaattt tgttgatgat
     3541 taagcatttg aaggttaagc aatagtaatc aaaatgcgcc tgatgataga caataaactt
     3601 tttgttttta tctggactgt gatatttata gttctaaatg gtttattatt tataagtgtt
     3661 gaaagagatt gaagaaaatg gcttcgtgac tcgctttctt cggtcttttt cgttttatta
     3721 tcaatttgta aacgatttac aatttgagtc aaattatggt aaaatagtgt catgagtaaa
     3781 atgtattatg cagaaaatcc tgacgctgct cacgacattc atgagttgag agtagagttg
     3841 ttgggacaaa aaatgacctt tttgacggat gcgggtgttt ttagcaagaa aatagttgac
     3901 tttggaagtc aactcttgct caagtgtctg gatgtcaacc aaggagagac agtcctagat
     3961 gtaggctgtg gttatggtcc attaggtttg tccttggtca aggtttacgg agttcaggcg
     4021 accatggttg atattaacac tcgtgccctg gatttagctc ggagaaatgc tgaaaaaaat
     4081 aatgcaaaag cgacgatatt tcaatctaat atttatgcaa gagttcaagg gcgatttgac
     4141 catgtcattt ccaatcctcc tatccgtgcg ggcaagcaag tcgttcatga aatcatcgag
     4201 aaaagtaaag atttcttgga aactggtggg gatttgacaa tcgtcatcca gaaaaaacaa
     4261 ggggctccaa gtgctaagtc caagatggag gatgtgtttg gtaattgtga aatccttaaa
     4321 aaagacaaag gatactatat ccttagaagt gtgaaagaat gagagcagtt gatttaatcc
     4381 aaaaaaaacg agacggtcaa gaactgactt caagtgaaat tgaatggctg atagaaggct
     4441 atgtgtcagg aactgttcct gattatcaga tgtctgcctt tgctatggct gtttatttta
     4501 aaggaatgac gactcgtgaa atttctgatt taacgatgaa tatggttaag actgggcaag
     4561 agtttgacct gtcagccatt gatggtgtga aagttgacaa gcattctact ggtggtgtcg
     4621 gcgataaggt aaccttgatt ttagctcctc ttgttgctag cttcggtgtg cctgtagcaa
     4681 aaatgagtgg tcgtggtctt ggccatacag gcggaacaat tgataaattg gagtccatta
     4741 agggctatca agtagaacgt agtcaagagg attttattcg tcaggttcag gatattggag
     4801 tatctgtcat tgggcaatca gaccagctgg tcaaagcaga taagcttctc tatgcccttc
     4861 gtgatgtgac cgcaactgtc gacacgattc ctttgattgc gagttcggtg atgagcaaga
     4921 aaattgcggc aggggcggat gctattttgc tagacgtgac tgtcggtgag ggtgccttca
     4981 tgaagacggt tgatgaggcg cgtgagttgg ctcaaactat ggtagatctt ggtaaggttg
     5041 ttggacggaa gacagtagca gtgattacgg atatgagcca gcctttggga cgagcgattg
     5101 gaaatcgtct ggaaatcctt gaagcattgg agattttgca aggtcaagga cgtcaggata
     5161 ttacccactt tatttgtgaa ttggctcaaa ttatgcttgg tttggcaaat gttaacaaaa
     5221 ctgttgaaga agttcgccaa catcttgaga atggtcaagc actggctaag tttgaggaaa
     5281 tggtgcaagc ccaaggtgga gacttggaag acctctattg tcctgtaaat gtagctcatg
     5341 tggtggaaat ccctgctcag gaaacgggtg tcatttcagc tcttccagct atggactttg
     5401 gtctttatgc catgaggctg ggtgctggtc gtgcagttaa gtctgatgcc ttggactatg
     5461 aaacaggaat tgtttttgaa aagaagattg gagactccgt tcaaaagggt gaaattgttg
     5521 caaaagtata cacaaatgaa aaaattccac ctcaactagt tacagatttc caaaaatgtg
     5581 ttaaaataag tgatgaagtg aaaaaaatcc gtgaaattgt agaaatcatc tcctaaatgg
     5641 agggaaaacc gaaatgaaat taaataaata tatagatcat acgcttttaa aacaagatgc
     5701 aaagaaaaaa caaattgata gtttgttgtc tgaggctaga gagtatgact ttgccagtgt
     5761 ttgcgttaat ccgacctggg ttgaacatgc taaaaaagga cttgaaggca cagatgttaa
     5821 ggtttgcaca gtagtaggtt tccccttggg agcaacaact tcaactgtga aagcttttga
     5881 aacaaaagaa gctatccaaa atggggctga tgagattgac atggtaatca atgttggggc
     5941 tctcaagtca ggtgatttgg ccttggttga gtcggatatt cgtgcagtag tggaagcaag
     6001 tggggacaag ttagtgaaag tcattattga agcttgcctt ctgacagacc aagaaaaagt
     6061 tttggcttgc caattagccc aaaaagcagg atctgacttt gtcaaaacat ctactggctt
     6121 ttcaactggt ggtgctacga tagcagatgt tagattaatg cgtgaaacag ttggatctga
     6181 tatgggagtc aaggctgctg gtggagctcg ttcttatgca gatgctcttg cctttgtcga
     6241 agcaggtgcg acccgtatcg gaacgtcagc tggggtagct attttaaaag gagaattggc
     6301 agatggcgac tactgagttg attgaactgg caattgaaac cagcaaacat gcctatgtcc
     6361 cctattctca ctttcctatc ggagcagttt tagtagccaa agacggaagt gtttacacgg
     6421 gagtgaatat cgaaaatgct agctatcctt tgaccaattg cggtgagaga acagctattt
     6481 ttaaggctat atctgaaggc caaagagaat tttcagaatt gattgtctat ggtcaaactg
     6541 aaaaaccgat ctcgccatgt ggtgcttgtc gccaagtgat ggctgaattt tttgaacaag
     6601 atctaaaagt gaccttagtc gcaaaagata aatcgacggt cgagatgacg gtcggggaat
     6661 tacttccata ctcttttaca gacttaaact agtctgagtc gctctctgag tggcacggtc
     6721 cttgtggcca accaatccat acttgcaaca tcgttgcaca tcttatttag gaggttcagt
     6781 aatgaacaag aaacaatggc taggtcttgg cctagttgca gtggcagcag ttggacttgc
     6841 tgcatgtggt aaccgctctt ctcgtaacgc agcttcatct tctgatgtga agacaaaagc
     6901 agcaatcgtc actgatactg gtggtgttga tgacaaatca ttcaaccaat cagcttggga
     6961 aggtttgcag gcttggggta aagaacacaa tctttcaaaa gataacggtt tcacttactt
     7021 ccaatcaaca agtgaagctg actacgctaa caacttgcaa caagcggctg gaagttacaa
     7081 cctaatcttc ggtgttggtt ttgcccttca caatgcagtt gaagaagcag ctaaagaaca
     7141 cactgacttg aactatgtct tgattgatga tgtgattaaa gaccaaaaga atgttgcgag
     7201 cgtaactttc gctgataatg agtcaggtta ccttgcaggt gtggctgcag caaaaacaac
     7261 taagacaaaa caagttggtt ttgtaggtgg tatcgaatct gaagttatct ctcgttttga
     7321 agcaggattc aaggctggtg ttgcgtcagt agacccatct atcaaagtcc aagttgacta
     7381 cgctggttca tttggtgatg cagctaaagg taaaacaatt gcagccgcac aatacgcagc
     7441 cggtgcagat attgtttacc aagtagctgg tggtacaggt gcaggtgtct ttgcagaggc
     7501 aaaatctctc aacgaaagcc gtcctgaaaa tgaaaaagtt tgggttatcg gtgttgaccg
     7561 tgaccaagaa gcagaaggta aatacacttc taaagatggc aaagaatcaa actttgttct
     7621 tgtatctact ttgaaacaag ttggtacaac tgtaaaagat atttctaaca aggcagaaaa
     7681 aggagaattc cctggcggtc aagtgatcgt ttactcattg aaggataaag gggttgactt
     7741 ggcagtaaca aacctttcag aagaaggtaa aaaagctgtc gaagatgcaa aagctaaaat
     7801 ccttgatgga agcgtaaaag ttcctgaaaa ataatggatg gaagtcattt cttaggaagc
     7861 ggccaagggc cgctttttta agagttgaga caaagtcatt ttgtctcagt aaagcttgct
     7921 actagacaaa ctagcaagtt ttattgaaat aaaataagac tctgaaagga agagcacatg
     7981 gcacacgaaa atgtcattga gatgcgtgat attaccaagg tgtttggtgg atttgttgcc
     8041 aacgacaaaa tcaacttgca cctacgaaaa ggtgaaattc atgcactttt aggagaaaat
     8101 ggggctggta agtccacgct aatgaacatg ttagcaggcc ttcttgaacc aactagtggt
     8161 gaaatcgcgg tcaacggtca agttgtcaat ctcgactccc catctaaagc agctagcttg
     8221 ggaatcggga tggttcacca gcactttatg ttggttgaag ccttcacagt ggctgaaaac
     8281 atcattttag gtagtgaatt gactaaaaat ggtgtgctag atatcgctgg agctagcaaa
     8341 gaaatcaagg ctctttctga acgttatggc ttagctgttg acccttctgc caaggtagta
     8401 gatatctcag ttggagccca acaacgtgta gaaattttaa aaacacttta taggggggct
     8461 gatatcctta tctttgacga accaacggct gttttgactc catcagaaat tgatgagttg
     8521 atggctatta tgaaaaatct tgtcaaagaa ggaaaatcaa ttatcttgat tacccacaaa
     8581 ttggatgaaa ttcgcgcagt ttctgaccgt gttacagtta tccgtcgtgg gaaatcaatt
     8641 gaaaccgttg aaattgcagg ggctaccaat gctgatttgg cggaaatgat ggtaggacgt
     8701 tctgtttcct ttaaaacaga gaagcaagcc tctaaaccaa aagaagtggt tttgtctatc
     8761 aaagatttgg tggtcaatga aaaccgtggt gttccagctg ttaaaaatct gtccttggat
     8821 gttcgtgctg gagagattgt tggtattgcg gggattgatg gaaatggtca gtctgaactg
     8881 attcaagcca ttacaggtct tcgtaaggtt gaatctggta gcattgagct aaaaggagat
     8941 tcaattgtag gcttgcaccc acgtcagatt acagaactaa gtgttgggca cgttccagaa
     9001 gaccgtcacc gtgatggctt gattttggaa atgatgatat ctgaaaatat tgcccttcaa
     9061 acctactata aagaaccaca tagtaaaaat ggaattttga attattcaaa tattacttct
     9121 tatgctaaaa agctgatgga agagtttgat gttcgcgctg ccagtgaatt agttcctgca
     9181 gctgcactct caggaggaaa tcaacaaaaa gcaattattg ctcgtgaaat tgatcgagat
     9241 cctgatctcc ttatcgttag ccagccaact cgtgggttgg atgtcggtgc cattgagtat
     9301 atccacaaac gcttgattga agagcgtgat aatggcaagg ctgtccttgt tgtcagcttt
     9361 gaattggatg aaattttaaa cgtctcagac cgtattgccg ttatccacga tggtaagatt
     9421 caaggtattg tatcaccaga aacaaccaat aaacaaggac ttggtgtctt gatggctggt
     9481 ggaaacttgg gaaaggagaa gagtgatgtc taaaaaatta caacaaattt cggttccctt
     9541 gatttctgta ttcctaggaa ttttactcgg agccattgtc atgtggatct tcggttatga
     9601 tgctatttgg ggctacgaag aattgttcta tacagccttt ggcagtctgc gtgggattgg
     9661 agaaatcttc cgtgctatgg gtcctctggt cttgattggt cttggttttg cggttgccag
     9721 tcgagctggt ttctttaacg tcggacttcc tggtcaggct ttggcaggtt ggattctcag
     9781 tggttggttt gccctgtcgc atccagatat gccccgtccc ttgatgattc tagcaaccat
     9841 cgtgattgcc ttgattgctg gtgggattgt cggagcgatt ccaggtattc ttagggccta
     9901 tctagggacg tcagaggtta ttgtaaccat catgatgaac tacattgtct tgtatgtagg
     9961 gaatgccttt atccatgctt tccctaaaga cttcatgcaa agtacagatt cgaccattcg
    10021 tgttggggct aatgcaacct atcagacacc ttggttggct gagttgactg gtaactcacg
    10081 gatgaatatt ggtattttct ttgccatcat tgccgttgca gttatttggt tcatgctcaa
    10141 gaaaacaact cttggttttg aaatccgtgc agttggtctt aatccacatg cttcagaata
    10201 tgctggtatt tctgccaagc ggactattat cctatctatg attatttcag gtgccttggc
    10261 aggtcttggt ggagctgttg aaggtttggg aaccttccag aacgtctatg ttcaaggttc
    10321 gtcattagct atcggattta acggaatggc ggttagtttg cttgcggcca actcaccaat
    10381 tggtatactc tttgcagcct tcctatttgg cgttctccaa gttggggctc ctggtatgaa
    10441 tgcggcgcag gtaccatctg agcttgtcag cattgtaaca gcgtctatta tcttctttgt
    10501 cagtgttcat taccttatcg aacgctttgt caaaccgaaa aaacaagtta aaggaggtaa
    10561 gtaaagatgt ctattataac cttgctcccc ctcttggtgt cttctatgct gatttactca
    10621 gcacctctca tctttacaag tatcggtggt gttttctctg aacgtggtgg tgtggtaaac
    10681 gttggccttg aaggaattat ggttatgggt gccttttctg gagttgtctt taaccttgaa
    10741 tttgcagaac aatttggagc agcaactcca tggctatcct tgcttgtagc aggattggtt
    10801 ggtagtgttt tctctatcat ccacgcagca gcgacggttc atttccgtgc agaccatgtt
    10861 gtcagcggta cggtattgaa cttgatggcg cctgccttgg ctgttttctt agttaaagtt
    10921 ctttataaca aaggacaaac cgacaaccta agtcaaactt ttggacgctt tgatttccca
    10981 gtcttggcaa atatcccagt gattggtgat atcttcttca agtcaactag tctacttggt
    11041 tatctggcga ttgccttctc attccttgct tggtttattc tctttaagac tcaatttggt
    11101 cttcgtctcc gctctgtcgg tgaacatcct caagcagcgg ataccttggg aatcaacgtc
    11161 tacaagatga gatatttagg ggttattatt tcaggttttc taggtggaat tggcggagcg
    11221 atttatgcgc aatcaatctc agttaacttc tcagtgacaa ctattgttgg acctggattt
    11281 atcgcccttg ctgcgatgat ctttgggaaa tggaatccaa tcggtgctat gctttctagt
    11341 ctcttctttg gactttcaca aagtttggct gttatcggtt ctcaattgcc gttcctacaa
    11401 ggagtgccag cggtttatct tcaaattgca ccttatgttt tgacaattct tgtcttagca
    11461 gccttctttg gaaaagcagt cgcaccaaaa gcagatggta tcaactacat caaatcaaaa
    11521 taagcataca aaaaaacgtc agtccaatga ctgaactgca ccccaaaagt tagacagaaa
    11581 aaatctaact tttggggtgt ttttattatg aaattaagtt atgatgataa agttcagatc
    11641 tatgaactaa gaaagcaagg atatagctta gagaagcttt caaataaatt tgggataaat
    11701 aattctaatc ttaggtatat gattaaattg attgatcgtt acggaataga gttcggcaaa
    11761 aaaggaaaaa atcgttacta ttctcctgat ttaaaacaag aaatgattca taaagtctga
    11821 catgaaggct ggactaaaga tagagtttct cttgaatacg gtctcccaag tcgtacgata
    11881 cttcttaact ggctagcaca atacaggaaa aacgggtata ctattgttga gaaaccaaga
    11941 gggagagtac ctaaaatggg acgtaagcca agaacgagac ctgaagagag gacagaatta
    12001 gaacgtcttc aagcagaaaa tgagtacctg agagcggaga atgccatcct aaaaaagtta
    12061 agagaactcc gattgaagga ggaaaaagag aaagaagaaa gacagaaatt gttcaagaat
    12121 taatgactga gttttcgtta gatcttcttc taaaagccat taaactagct cgttggacct
    12181 actactatca cttgaaacag ctagacaaaa cagataagga ccaagagctt aaagctgaaa
    12241 ttcaatccat ttttatcgaa cacaaaggaa attatgctta tcgccgggtt catttagaac
    12301 taagaaatcg tgcttatctg gtaaatcata aaagagttca aggcttgata aaagtactca
    12361 taatttagaa caagtacaaa caatgttgga acaggcattc aaagagaagc actacgagaa
    12421 tacgattctc catagtgacc aaggctggca atatcaacac gattcttatc atcggttcct
    12481 agagagtaag ggaattcaag catccatgtc acgtaagggt aacagcccag acaatggtat
    12541 gatggagtcc ttctttggga ttctgaaatc ggaaatgttt tacggttatg agaagtcgtt
    12601 tcagtcgttt aagcaattgg aacaagctat tgtagactat attgattact acaacaacaa
    12661 acgaattaag gtaaaactaa aaggacttag tcctgtgcag tacagaacta aatcctttgg
    12721 ataaattatt tgtctaactg tttgggggca gtacagaact ggcgttttat tttttaggat
    12781 cttgatgagt taggttcaat ccccaaggga ccaaattttc agttttattt ttgatacgag
    12841 ctatattgtc cttatgacga atgataatca aactagcaag tgctaagata atagcgatga
    12901 agagagagtc atagttactc aggataaaac caaaaagtgg aaagagcaga accccgataa
    12961 cagccgcgat cgatgctgtg acactagaca gtgaaatcat actgccaaga tagagagctc
    13021 caaagaagat aatcgcaagg tagagacaga agataggcgc aaatccgaaa atcactccag
    13081 cactggttgc gacagcctta ccacctttaa atcctgcaaa gatagggaag gtatggccga
    13141 taacagccaa aagtccaaag atgagaggag aaacgccttg tagatgaaaa ataatcggaa
    13201 gcagcgttgc tagggttcct ttgaaaaagt caatcacaaa ggttgccata ccagctttct
    13261 tacctaaaat gcggaaggta ttggtcgttc cagtgttacc agaaccatgc tcgcgtagat
    13321 tgatttgaaa gaatacttgt ccaatccaga gaccagatgg aatcgaaccc agcagatagg
    13381 ctaggattaa taaaattatt gtaatcatac tcctattata tcatgaattg ggaaagaaaa
    13441 gcagagaatc tctgctgaaa ttgtcacatc ggagaaagaa aaattttgca aaatccttgg
    13501 aaaacctgta gaatagtaaa gatgaacgaa taggaggttc cttgtgtcaa aaaaggaaat
    13561 caatattaac aattataatg atgatgctat tcaggtgcta gaagggttgg atgcggtccg
    13621 aaaacgtcca gggatgtata ttggatcgac cgatggcgct ggtcttcatc acctagtttg
    13681 ggaaatcgtt gataatgcag tcgatgaagc cttgtctggg tttggtgatc gtattgatgt
    13741 aactatcaat aaagacggta gtctaacggt tcaagaccat ggacgtggga tgccgacagg
    13801 tatgcacgct atgggaattc caactgttga ggttatcttt accattcttc atgccggagg
    13861 gaaattcggt caaggtggct ataagacatc aggtggactt cacggagtgg gttcttccgt
    13921 tgttaacgcc ctttctagct ggttagaagt tgaaattacc cgtgatggcg cagtttacaa
    13981 gcaacgtttt gaaaatggtg gaaaacctgt cacgactttg aagaaagtcg gtacagcacc
    14041 caagtctaaa acaggcacca aagttacttt tatgcctgac gcgactatct tttctacgac
    14101 agatttcaag tacaatacca tttcagagcg ccttaatgaa tcagcctttc tcttgaaaaa
    14161 tgtgaccttg tctttaacgg acaagcgatc agatgaagcg attgagttcc actatgagaa
    14221 tggagtacaa gattttgttt cttatctcaa cgaagataag gaaatcttga cgccagttct
    14281 ttactttgaa ggggaagaca atggttttca agtggaagta gccctccagt acaatgacgg
    14341 attctcagat aacattctat cctttgtcaa taacgttcgc accaaggacg gtggaacgca
    14401 cgagacagga ctcaagtctg ccattaccaa ggtcatgaat gactatgcac gtaaaacagg
    14461 tcttctcaag gaaaaagata aaaaccttga aggttcagac tatcgtgagg gactagcggc
    14521 cgttctttct atcttagttc ctgaagaaca cttgcagttt gaaggacaga ccaaggataa
    14581 actaggaagc cccttagctc gcccagttgt ggatggaata gtggctgata agttgacctt
    14641 tttccttatg gaaaatgggg aattagcttc taacctcatc cgcaaggcta tcaaggcccg
    14701 tgatgctcgt gaagcagcac gtaaggcgcg tgatgagagc cgaaatggga agaaaaacaa
    14761 gaaagataag ggcttgttgt ctgggaaatt gaccccagcc caatctaaga atcctgctaa
    14821 gaatgaactc tatctagttg agggggactc tgccggtggt tctgccaaac aaggtcgtga
    14881 ccgcaagttc caggctattc tacctcttcg tggtaaggtt atcaatacag ccaaggccaa
    14941 gatggcggat atcctcaaaa atgaagagat caataccatg atttatacca ttggtgcggg
    15001 tgttggagca gacttctcta ttgaagatgc caactatgat aagatcatta tcatgaccga
    15061 tgcggatacc gacggtgccc atatccagac cttgctcttg acatttttct accgttacat
    15121 gcgtccgcta gtcgaggcag gtcatgtcta tattgccctc ccacctcttt acaagatgtc
    15181 caaaggtaaa ggcaagaaag aagaagtggc ctacgcttgg acggacggag aactagaaga
    15241 actacgtaaa cagttcggta aaggcgctac cctccaacga tacaaaggac ttggtgagat
    15301 gaatgcggac cagctctggg aaacaaccat gaacccagaa acacgtaccc tcatccgtgt
    15361 cacaattgaa gatttagcgc gcgccgaacg tcgcgtcaat gttctcatgg gagataaggt
    15421 agaaccacgc cgtaaatgga ttgaagataa tgtcaagttt acgctagaag aagcgacagt
    15481 gttttaatga aagaaaataa tatgttagta aactggaaat tagagtcgga tgtgaatgac
    15541 tatgtgaaaa agcagtttga aaatcttggt ttgaaaaaat tgcagaatta ttaatgaaga
    15601 atcttctatg agtgcttatc tcaaagaggc gtttaaggga gcggctaaga cacaaactaa
    15661 gactaatttt ggaaaaccag attttcatat tgaaaaatac aaacaggata gtttggttac
    15721 ttatcgtaac tgaacacggg acgaattccc gtaaaatatc ttttactgca taaaaaccta
    15781 ctctacattc tttgaaagga gttgaacacg ccctagatat tgtgtgaaaa agataaattc
    15841 tcttgtgagt ttgcttactt caagaatttt ctattttcac ttggtatttt atgggctttg
    15901 tatcttatgt ctaacattca aaacatgtcc ctggaggaca tcatgggaga gcgctttggt
    15961 cgctactcca agtacattat tcaagaccgg gctttgccag atattcgtga tgggttgaag
    16021 ccggttcagc gccgtattct ttattctatg aataaggata gcaatacttt tgacaagagc
    16081 taccgtaagt cggccaagtc agtcgggaac atcatgggga atttccaccc acacggggat
    16141 tcttctatct atgatgccat ggttcgtatg tcacagaact ggaaaaatcg tgagattcta
    16201 gttgaaatgc acggtaataa cggttctatg gacggagatc ctcctgcggc tatgcgttat
    16261 actgaggcac gtttgtctga aattgcaggc taccttcttc aggatatcga gaaaaagaca
    16321 gttccttttg catggaactt tgacgatacg gagaaagaac caacggtctt gccagcagcc
    16381 tttccaaacc tcttggtcaa tggttcgact gggatttcgg ctggttatgc cacagacatt
    16441 cctccccata atttagctga ggtcatagat gctgcagttt acatgattga ccacccaact
    16501 gcaaagattg ataaactcat ggaattcttg cctggaccag acttccctac aggggctatt
    16561 attcaaggtc gtgatgaaat caagaaagct tatgagactg ggaaagggcg cgtggttgtt
    16621 cgttccaaga ctgaaattga aaagctaaaa ggtggtaagg aacaaatcgt tattactgag
    16681 attccttatg aaatcaataa ggccaatcta gtcaagaaaa tcgatgatgt tcgtgttaat
    16741 aacaaggtag ctgggattgc tgaggttcgt gatgagtctg accgtgatgg tcttcgtatc
    16801 gctatcgaac ttaagaaaga cgctaatact gagcttgttc tcaactactt atttaagtac
    16861 accgacctac aaatcaacta caactttaat atggtggcga ttgacaattt cacacctcgt
    16921 caggttggga ttgttccaat cctgtctagc tacatcgctc accgtcgaga agtgattttg
    16981 gcgcgttcac gctttgacaa agaaaaggct gagaaacgtc tccatatcgt cgaaggtttg
    17041 attcgtgtga tttcgatttt ggatgaagtc attgctctta tccgtgcttc tgagaataag
    17101 gcggacgcca aggaaaacct caaagttagc tatgatttta cggaagaaca ggctgaggct
    17161 atcgtaactt tgcaactgta ccgtttgacc aataccgatg tggttgtctt gcaggaagaa
    17221 gaagcagagc ttcgtgagaa gattgctatg ctggcggcta ttattggtga tgaaaggact
    17281 atgtacaatc tcatgaagaa agaacttcgt gaggtcaaga agaaatttgc aactcctcgt
    17341 ttgagttctt tagaagacac tgcgaaagca attgagattg atacagctag tcttatcgct
    17401 gaggaagata cctacgtcag cgtgaccaag gcaggttaca tcaagcgtac cagtccacgt
    17461 tcctttgcgg cttccacctt ggaagaaatt ggcaagcgtg atgatgaccg tttgattttt
    17521 gttcaatctg ccaagacaac ccagcacctc ttgatgttca caagtcttgg aaatgtcatc
    17581 tacagaccaa tccatgagtt ggcagatatt cgttggaagg acatcggaga gcatctgagc
    17641 caaaccatca caaactttga aacgaatgaa gaaatccttt atgtggaagt actggatcag
    17701 tttgacgatg cgacaaccta ctttgcagcg actcgccttg gtcaaatcaa acgggtagag
    17761 cgaaaagaat tcactccatg gcggacctat agatctaagt ctgtcaagta tgctaagctc
    17821 aaagacgata cagatcagat tgtagcagtg gctccgatta aactagatga tgttgtcttg
    17881 gttagtcaaa atggttatgc cctgcgtttc aatatcgaag aggttccggt tgtcggtgct
    17941 aaggcagcag gtgtcaaggc tatgaatttg aaagaagatg atgtcctcca atctggcttt
    18001 atctgtaata cttcgtcctt ctacctcttg acccagcgtg gaagcttgaa acgtgtttcc
    18061 attgaggaaa ttctagcaac cagccgtgcc aaacgaggat tacaggtctt gcgtgagttg
    18121 aaaaacaaac cgcatcgtgt cttcttggca ggagcagttg cagagcaagg atttgttggc
    18181 gatttcttca gtacggaagt ggatgtgaac gaccaaactc tgcttgtcca atccaataaa
    18241 ggaacaatct atgaaagccg attgcaagac ttgaacttgt cagaacgcac tagcaatgga
    18301 agcttcattt ctgacacgat ttcagatgaa gaagtttttg acgcttatct tcaggaagta
    18361 gttactgaag ataaataagt gaaatgaaga atcagttcta agagctggtt tttattggag
    18421 aaattttctg aaaattacaa aatatacttg ctattttaga aaaatagtgt agaatataag
    18481 aaataggttt ttagaataag gagtggaata tgacagtaac gattgattgg gaaaacctcg
    18541 gtttttccta tatgaaatta ccttatcgct atcttgctca tttcaaaaat ggacaatggg
    18601 atcaaggaga gcttacagag gatgcaactt tgcatatttc agagtcttct ccaagtcttc
    18661 actatggaca acaagcattt gaaggtttga aagcttatcg tactaaggat ggcagtgttc
    18721 aactgttccg tcctgatgaa aatgctaaac gcctgcaacg tacatgtgac cgtctcttga
    18781 tgccacaagt tccgacagac atgtttgtag aagcttgtaa agcagttgtc cgtgcgaatg
    18841 aagaatacgt accaccatac ggaataggtg gaaccttata tcttcgccct cttttgattg
    18901 gtgtcggaga tattatcggg gtaaaaccgg cagaagagta cattttcacc atctttgcta
    18961 tgccagttgg aaattacttt aaaggtggtt tggtcccaac caacttcttg attcaggatg
    19021 agtacgaccg tgcagcacca aatggtacag gtgcggctaa ggttggtgga aactatgctg
    19081 caagtctctt accaggaaaa atggccaagt cacgtcattt ctcagatgtt atctatctgg
    19141 acccatcaac tcatacaaag attgaagaag tcggatcagc taacttcttt ggaattacag
    19201 ctgataatga atttgtaaca ccattgagtc catctatctt gccatctatt accaagtatt
    19261 ccttgcttta tttggcagaa caccgcttgg gattaactcc tattgagggt gatgttccaa
    19321 ttgataatct tgaccgtttt gtagaggcag gtgcctgtgg tacagcagcg gttatttctc
    19381 caattggagg tattcaacat ggtgatgatt tccatgtatt ctatagtgaa acagaagtag
    19441 gtcctgtgac gcgtaaatta tataatgaat tgacgggtat tcagtttggc gatattgaag
    19501 cgccagaagg ttggattgta aaagtagatt aaaataaacc gaaggagatt ttttatgaaa
    19561 tagaaaaagt ggctcttaac agcaggagtg gtcctgagca cgtcagctat tttagtggct
    19621 tgtggaaaaa ctgataaaga agccgatgca ccgacaacat tttcttatgt ctatgcagta
    19681 gatccagcat cattgggcta cagtatagcg actcgaacat cgacgacaga cgttattgga
    19741 aatgttattg atggtttgat ggaaaatgat aaatacggca atgttgctcc ttctcaaaaa
    19801 gactatgatt tgaatagtac aggatgggct ccaagctatc aagatccagc gtcttacttg
    19861 aatattatgg atccaaaatc tggttctgcc atgaaacacc ttggtattac gaaaggaaaa
    19921 gataaggatg ttgtagctaa acctggtttg gataaatata agaaattgtt agaagatgct
    19981 gtttctgaga ccactgacct agagaagaga tatgaaaaat atgccaaagc tcaagcttgg
    20041 tcgacagata gttcattatt gatgccaaca gcttcatctg gtggttctcc agttgtaagt
    20101 aacgtagtac cattctcaaa accatactca caagttggta ttaaggggga tccatatatc
    20161 tttaaaggaa tgaaattgca aaaagatatt gttacaacaa aagaatataa cgaggttttt
    20221 aaaaaatggc aaaaagaaaa attggaatcc aatagcaaat accaaaaaga actagaaaaa
    20281 cacattaaat aaggaatggt attgatcttg ataaaatttt aaaaatactg tcattttgaa
    20341 tataaaggag tttgatatgg agtggattag attaatagga atagcaatca ttgttgtggg
    20401 ttttatttta aaatttgata caattgcaac agtagtctta gctggtttgg ttacagcttt
    20461 agtttcaggt gtttctctcg ttgaattttt ggagattttg ggaaaagaat ttagcaatca
    20521 gcgagtgctc acgattttta tggttacctt gcctcttgtg gggctgtcag aaacctttgg
    20581 actcaagcaa cgatcaatcg atttgattcg aaagattaaa ggtctgacag ttggaaactt
    20641 ctatacagtt tatttcttta ttcgagagtt agctggtttc ttttcaattc gtctaggagg
    20701 acaccctcag tttgtcagac ctttggttca gcctatggga gaagcagctg cagagtctca
    20761 attaggtaga aagttaacag aggttgaaga tgagacaata aaagcgcgtg cggcagcgaa
    20821 tgaaaatttt ggaaatttct ttgctcaaaa tacgtttgta ggtgctgggg gagtcctctt
    20881 gataggggga acattagatc agttaggcta tgaaagcaat tatgcaggga ttgcttctac
    20941 atctattatt gttgctgtta taacacttat tgtagtgggg atttacaatt atttatttga
    21001 taaaaaattg atatcaaaaa agactagggg aggagaacaa aaatgacaga gttggcaaag
    21061 caactattag agttgaccta tattgtgatt ggttgtcaat ttctccatac agcctattgt
    21121 agttataaag ataaaacaaa cccagttcga cttgggacat ctgcattttg gactctattg
    21181 tctattacgt ttataggtgg ttcctatatg ccaaatatga gtattggtat tattgtaatc
    21241 ctattatcgc tgttaacatt gtttaagcaa gtccgtatcg gaaccttgcc atccttagat
    21301 gaaatgaaag ccaatattga atctaacagg ttgaaaaata aaatttttat tccagttatg
    21361 ctgatggcaa tacttgcgtt ggtcttagcg caaatgattc cagaatttag caagatttcg
    21421 attagccttg ccgccttgtt tgctacaatt tctgttcttg tgattaccaa tagtcaccct
    21481 aagagtctgt tatcagaaaa taatcgaatg actcagcaag tttcaacaag tggtattgtt
    21541 cctcaattat taggggcttt gggggctatt tttactgtag caggtgttgg tgatgttatc
    21601 tctcatctga ttagcggtat tgttccttca gatagtcgct ttataggagt tttggcctat
    21661 gttcttggaa tggttctatt cacaatgatt atgggaaatg cttttgcagc attcaccgtt
    21721 attacagcag gtgttggagt tccctttgta tttgctctgg gagctaatcc aattgtggct
    21781 ggtgctcttg ccatgacagc aggttattgt gggaccttat tgaccccaat ggctgctaat
    21841 tttaacgctc taccagcagc attgatggat atgaaagatc agaatggcgt tataaaggct
    21901 caagcaggtg ttgctctagt aatgattgtt attcacatat tcttaatgta ctttctcgca
    21961 ttttagtaaa ggaaaataaa atgaaaatat tggttacagg ttttaatcct tttggaggtg
    22021 aaaagattaa tccagctttg gaggctgtaa aattattacc atctgagatt aatggggctg
    22081 aagttcgctg ggtagaaatt ccaacggttt tttataagtc gtcagaagtt ttagaggcag
    22141 aaatattacg atatcaacca gatgctgtac tttgtattgg acaagcaggc ggcaggaccg
    22201 gcttaacacc tgaacgagtg gctattaatc aagatgatgc tcgcatacct gataacgaag
    22261 gcaatcaacc aattgataca ccgattcgta ttgatggagc atcggcctat tttagtagtt
    22321 tacctatcaa agcgatggta caagctatca aaaaacaggg acttccggca gttgtatcca
    22381 atagtgcagg aacctttgtt tgcaatcatt tgatgtacca agctctttat ttagtggata
    22441 aaaaattccc taatatgaga gcaggtttca tgcatattcc atatatgatg gaacaagtag
    22501 taaataagcc gaatactgca ggaatgagtc tatgtgatat tgtaagaggc atagaggtag
    22561 ctattgaagc tattgtagac tacaaagata aggatttgca gttagtaggc ggtgagactc
    22621 actgataacc taaaaaggat agtcaattat gcttgtttac taactattaa ctatgctaaa
    22681 tcaattgagg ttgtttacat aaaactctat atcagagaag cctgatatag agttttttct
    22741 tgctagtttt agaattttct tgtaaaatag aaaaagtgaa gagaggtatg aaatgagcaa
    22801 gaaagataaa aaaatcgaaa ttcaagtagc ggatgccaaa gttaatgttg gtaaagacag
    22861 ttttgaaggt tatacattga ctatcggtaa aaaagttatc ggagaaattg ccgaattaga
    22921 cggacaattt gccattataa agaatgggaa tgtcgatagt ttttataaaa aattggaaaa
    22981 agctgtggaa attttgattg aaaattataa tttagcaaaa taagtcttgt ttttttgaaa
    23041 ttttcatgat ataatagtcc atgttgattg taggagagat agcgaagagg ctaaacgcgg
    23101 cggactgtaa atccgctcct tcgggttcgg gggttcgaat ccctctctct ccatttcatt
    23161 aatggggtat agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa
    23221 tccagctacc ccagttctta ggtaataatc aagataaaaa gcaaaatatc ttagggtatt
    23281 ttatttttat aattgaaaga cgtgaatgat atgaacatgt ccttgcgggt gcttaggaaa
    23341 aaaattataa gtatgtcaag tttaagaaaa acttgattgt tggaggattt tttagatgaa
    23401 cgaatttgaa gatttgctaa atagcgttag tcaagttgag actggtgatg ttgttagtgc
    23461 tgaagtattg acagttgatg cgactcaagc taacgttgca atctctggaa ctggtgttga
    23521 aggtgtcttg actcttcgcg aattgacaaa cgatcgtgat gcagatatca atgactttgt
    23581 taaagtagga gaagtattgg atgttcttgt acttcgtcaa gtagttggta aagatactga
    23641 tacagttaca taccttgtat ctaaaaaacg ccttgaagct cgcaaagcat gggacaaact
    23701 tgttggtcgc gaagaagaag ttgttactgt taaaggaacg cgtgccgtta aaggtggact
    23761 ttcagtagaa tttgaaggtg ttcgtggatt tatcccagct tcaatgttgg atactcgttt
    23821 cgtacgtaat gctgagcgtt ttgtaggtca agaatttgat actaaaatca aagaagttaa
    23881 cgctaaagaa aaccgcttca tcctttcacg tcgtgaagtt gttgaagcag ctactgcagc
    23941 agctcgcgct gaagtattcg gtaaattggc tgttggtgat gttgtaactg gtaaagttgc
    24001 tcgtatcaca agcttcggcg ctttcgtcga ccttggtggt gttgacggat tggttcactt
    24061 gactgaattg tcacatgaac gtaacgtatc accaaaatca gttgtaactg ttggtgaaga
    24121 aattgaagtg aaaatccttg atcttaacga agaagaagga cgtgtatcac tttcacttaa
    24181 agcaacagta ccaggaccat gggatggcgt tgagcaaaaa ttggctaaag gtgatgtagt
    24241 agaaggaaca gttaaacgtt tgactgactt cggtgcattt gttgaagtat tgccaggtat
    24301 cgatggactt gttcacgtat cacaaatttc acacaaacgg attgaaaatc caaaagaagc
    24361 tcttaaagtt ggtcaagaag ttcaagttaa agttcttgaa gttaacgcag atgcagaacg
    24421 cgtgtcactt tctattaaag ctcttgaaga acgtccagcc caagaagaag gacaaaaaga
    24481 agaaaaacgt gctgctcgtc cacgtcgtcc aagacgtcaa gaaaagcgtg atttcgaact
    24541 tccagaaaca caaacaggat tttcaatggc tgatttgttt ggtgatatcg aactttaatc
    24601 aaattgaaaa ttcacaaaat cctttgttta ctaaacaagg gatttttct
//
LOCUS       DAVKLX010000031        24113 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKLX010000031 DAVKLX010000000
VERSION     DAVKLX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24113
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.031"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            2..74
                     /locus_tag="VW325_001345"
     tRNA            2..74
                     /locus_tag="VW325_001345"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            99..171
                     /locus_tag="VW325_001346"
     tRNA            99..171
                     /locus_tag="VW325_001346"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Lys,seq:ttt)
     gene            177..258
                     /locus_tag="VW325_001347"
     tRNA            177..258
                     /locus_tag="VW325_001347"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Leu,seq:tag)
     gene            277..349
                     /locus_tag="VW325_001348"
     tRNA            277..349
                     /locus_tag="VW325_001348"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Thr,seq:tgt)
     gene            372..443
                     /locus_tag="VW325_001349"
     tRNA            372..443
                     /locus_tag="VW325_001349"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Gly,seq:gcc)
     gene            451..536
                     /locus_tag="VW325_001350"
     tRNA            451..536
                     /locus_tag="VW325_001350"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:taa)
     gene            546..619
                     /locus_tag="VW325_001351"
     tRNA            546..619
                     /locus_tag="VW325_001351"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:580..582,aa:Arg,seq:acg)
     gene            635..708
                     /locus_tag="VW325_001352"
     tRNA            635..708
                     /locus_tag="VW325_001352"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:669..671,aa:Pro,seq:tgg)
     gene            714..787
                     /locus_tag="VW325_001353"
     tRNA            714..787
                     /locus_tag="VW325_001353"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Met,seq:cat)
     gene            801..874
                     /locus_tag="VW325_001354"
     tRNA            801..874
                     /locus_tag="VW325_001354"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Ile,seq:cat)
     gene            885..974
                     /locus_tag="VW325_001355"
     tRNA            885..974
                     /locus_tag="VW325_001355"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Ser,seq:tga)
     gene            985..1058
                     /locus_tag="VW325_001356"
     tRNA            985..1058
                     /locus_tag="VW325_001356"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1019..1021,aa:Met,seq:cat)
     gene            1062..1134
                     /locus_tag="VW325_001357"
     tRNA            1062..1134
                     /locus_tag="VW325_001357"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1095..1097,aa:Phe,seq:gaa)
     gene            1154..1224
                     /locus_tag="VW325_001358"
     tRNA            1154..1224
                     /locus_tag="VW325_001358"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Gly,seq:tcc)
     gene            1260..1333
                     /locus_tag="VW325_001359"
     tRNA            1260..1333
                     /locus_tag="VW325_001359"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1294..1296,aa:Ile,seq:gat)
     gene            1340..1427
                     /locus_tag="VW325_001360"
     tRNA            1340..1427
                     /locus_tag="VW325_001360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1374..1376,aa:Ser,seq:gct)
     gene            complement(1531..2832)
                     /locus_tag="VW325_001361"
                     /pseudo
     CDS             complement(1531..2832)
                     /locus_tag="VW325_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2978..3913)
                     /locus_tag="VW325_001362"
     CDS             complement(2978..3913)
                     /locus_tag="VW325_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9734744.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLQQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(3910..4770)
                     /locus_tag="VW325_001363"
     CDS             complement(3910..4770)
                     /locus_tag="VW325_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9734745.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKDKIVYLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            4878..7040
                     /locus_tag="VW325_001364"
     CDS             4878..7040
                     /locus_tag="VW325_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9734746.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGV
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            7132..8390
                     /locus_tag="VW325_001365"
                     /pseudo
     CDS             7132..8390
                     /locus_tag="VW325_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            8404..9270
                     /locus_tag="VW325_001366"
     CDS             8404..9270
                     /locus_tag="VW325_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9734747.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            9294..10127
                     /locus_tag="VW325_001367"
     CDS             9294..10127
                     /locus_tag="VW325_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9734748.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     VFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            10128..10208
                     /locus_tag="VW325_001368"
                     /pseudo
     CDS             10128..10208
                     /locus_tag="VW325_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            10485..11927
                     /gene="gtfA"
                     /locus_tag="VW325_001369"
     CDS             10485..11927
                     /gene="gtfA"
                     /locus_tag="VW325_001369"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9734749.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            12065..13258
                     /locus_tag="VW325_001370"
     CDS             12065..13258
                     /locus_tag="VW325_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9734750.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            13944..15923
                     /locus_tag="VW325_001371"
     CDS             13944..15923
                     /locus_tag="VW325_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9734751.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            15990..17486
                     /locus_tag="VW325_001372"
     CDS             15990..17486
                     /locus_tag="VW325_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734752.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            17486..18412
                     /locus_tag="VW325_001373"
     CDS             17486..18412
                     /locus_tag="VW325_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734753.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            18421..19488
                     /locus_tag="VW325_001374"
     CDS             18421..19488
                     /locus_tag="VW325_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734754.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGELYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSKTHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            19499..20425
                     /locus_tag="VW325_001375"
     CDS             19499..20425
                     /locus_tag="VW325_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734755.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            20992..21852
                     /locus_tag="VW325_001376"
     CDS             20992..21852
                     /locus_tag="VW325_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9734756.1"
                     /translation="MSQFITHQSLYATREGFVDELAKQGYVEGENIEIDLQNAQGEQR
                     NLKTISQQLAESSDVVLAIARPSAQSLANTTQTTPVIFSAVTDPVSAKLVESREHPGG
                     NVTGTSDQSSDAISTQINLIKKVLPKAKTIGILYTQSEPNSVVQKDEAKRLLKEKGFT
                     VVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISLTMETVKQVSIKHKVPVFGGSTE
                     MIAVGGLYNYGTNYEELVRQTARMLIRVLKGEKPENIAVELPEKLELHTNQEMADALG
                     IDISKLEGKE"
     gene            21864..22757
                     /locus_tag="VW325_001377"
     CDS             21864..22757
                     /locus_tag="VW325_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734757.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            22770..23573
                     /locus_tag="VW325_001378"
     CDS             22770..23573
                     /locus_tag="VW325_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9734758.1"
                     /translation="MTSLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(23606..>24113)
                     /locus_tag="VW325_001379"
     CDS             complement(23606..>24113)
                     /locus_tag="VW325_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HEU9734759.1"
                     /translation="EETVNFPVSNLTSYQELVKELGLRSKTNVVSLDKIIIDKKSSLI
                     TGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIYSYIENGLKLL
                     IDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTESRHKLEKFRF
                     VDFAKRKK"
BASE COUNT         7004 a         4304 c         5289 g         7516 t
ORIGIN      
        1 aggtcccgta gtgtagcggt tatcacgtcg ccctgtcacg gcgaagatcg cgggttcgat
       61 tcccgtcggg accgtttaag gtaacgcaag ttattttaga ctcgttagct cagttggtag
      121 agcaattgac ttttaatcaa tgggtcactg gttcgagccc agtacgggtc atatatgcgg
      181 gtttggcgga attggcagac gcaccagatt taggatctgg cgcttaacgg cgtgggggtt
      241 caagtccctt aacccgcatt aagatataac aaatgagccg gcttagctca gttggtagag
      301 catctgattt gtaatcagag ggtcgcgtgt tcaagtcatg tagccggcat ttttagatag
      361 aagaaaacag tgcgaacgta gttcagtggt agaacaccac cttgccaagg tgggggtcgc
      421 gggttcgaat cccgtcgttc gcttagagag gccggggtgg cggaactggc agacgcacag
      481 gacttaaaat cctgcgattg gtaacgatcg taccggttcg attccggtcc tcggcataat
      541 atggagcacc cttagctcaa ctggatagag tacctgacta cgaatcaggc ggttagaggt
      601 tcgactcctc tagggtgcat ttttctattt aactcgggaa gtagctcagc ttggtagagt
      661 acttggtttg ggaccaaggt gtcgcaggtt cgaatcctgt cttcccgatt catggcggtg
      721 tagctcagct ggctagagcg tccggttcat acccgggagg tcgggggttc gatccccttc
      781 gccgctataa tgatcttgtc ggacctttag ctcagctggt tagagctctc ggctcataac
      841 cgagtggtcg taggttcaag tcctacaagg tccatttgaa tattggagga ttacccaagt
      901 ccggctgaag ggaacggtct tgaaaaccgt caggcgtgta aaagcgtgcg tgggttcgaa
      961 tcccacatcc tccttttata ttaacgcggg atggagcagc tcggtagctc gtcgggctca
     1021 taacccgaag gtcgtaggtt caaatcctgc tcccgcaata aggctcggta gctcagttgg
     1081 tagagcaatg gattgaagct ccatgtgtcg gcggttcgat tccgtctcgc gccattttat
     1141 tgatagtatt tatgcgggtg tagtttagtg gtaaaactac agccttccaa gctgttgtcg
     1201 cgagttcgat tctcgtcacc cgctttgaac tttgttcaaa ttaccaagtt ttttaacttg
     1261 ggcgcgtagc tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt
     1321 ccactcgtgc ccattaattg gagaattact caagaggctg aagaggacgg tttgctaaat
     1381 cgttaggtcg ggtaactggc gcaagggttc gaatccctta ttctccgttt taatgagagt
     1441 ttgtaactct cttttttgtt tttagagaca ataaaacttc agatccgtaa acttaaggct
     1501 ctttgtcaac tgtagtgggt tgaagaaaag ctaagctcga gaaaggacaa atttcgtcct
     1561 ttcttttttg atgttcagag cgatgaaaat ccgttttttg aagttttcaa agttccgaaa
     1621 accaaaggca ttgcgcttga taagtttgat gagattattg gtcgcttcca gtttggcatt
     1681 agaatagtgt agttgaaggg cgttgacaat cttttcttta tctttgagga aggttttaaa
     1741 gacagtctga aaaataggat gaacctgctt aagattgtcc tcaataagtc cgaaaaattt
     1801 ctctggttcc ttattctgaa agtgaaacag caagatagag ctgatagtgg tgtttcaagt
     1861 cttgtgaata gctcaaaagc ttgtctaaaa tctctttatt ggttaaatgc atacgaaaag
     1921 tagggcgata aaaatgttta tcgctgagtt tacgactatc ctgttgaatg agtttccagt
     1981 agcgcttgat agccttgtat tcatgggatt ttcgatgaaa ctgattcatg atttgaacac
     2041 gcacacgact catagcacgg ctaagatgtt gtacaatgtg aaagcgatca agaacgattt
     2101 tagcattcgg gagtgaaaca gtctgggaga ctgtttcagc ctgagcctag aaatttgaaa
     2161 gcgaagctgt ttagccaagt catagtaagg gctaaacata tccatagtaa tgattttgac
     2221 gcgacatcgg acagctctat cgtagtgaag aaagtgattt cgaatgatag cttgtgttct
     2281 accctcaaga acagtgatga tattgagatt gttaaaatct tgcgcaatga agctcatctt
     2341 tccctttgta aaagcatact catcccaaga cataatctca ggaagacaag aaaaatcatg
     2401 tttaaagtga aaatcattga gcttacgaat aacagttgaa gttgaaatgg ccagctgatg
     2461 agcaatatcg gtcatagaaa tcttctcaat caacttttgc gcaatttttt ggttgatgat
     2521 acgagggatt tggtgattct tcttgacgat agaagtttca gcgaccatca tttttgaaca
     2581 gtgatagcac ttgaaacggc gttttctaag gagaattcta gtaggcatac cagtcgtttc
     2641 aagataagga attttagaag gtttttgaaa gtcatatttc ttcaattggt ttccgcactc
     2701 agggcaagat ggtgcgtcgt agtccagttt ggcgatgatt tctttgtgtg aatccctatt
     2761 gacgacatct ataatttgga tatttgtgtc tttgatatcg agtagttttg tgataaaatg
     2821 taattgttcc atatgaatct ttctaatgag ttgtttgatc gcttttcatt atagatctta
     2881 tgggactttt tttctacaca aaaataggct ccataatatc cataggggat ttacccacta
     2941 caaatattat agagccttat attataattg tgttatttta cttccaacta ttgagagaaa
     3001 tttcgccact atttagccag atttcttttc cgttatcaca ttgaactaaa agttttccat
     3061 tttctgagat gtctttagca agtcccttgt agtctttttg ctgtagtgtg aaagtgactt
     3121 cttttcctag aatgaatgac tgttttttgt ataggtataa tagctcttct gctggtgttt
     3181 cgaagaaagc acgccagatt tctatgatca attcattcct tgttatagga gctgtagctt
     3241 taaataagct ggcagctttt tcttttaatt cctgagggaa gtctttaata gtgaaattga
     3301 tacctactcc aataatgata tctgtgacta agccagtttc tacagaggtc attgcttcag
     3361 taaggattcc tccaatttta tgattgttta gatagatatc attgacccat tttatgtcga
     3421 catctattaa agttaggttc ttaatagctt tgtagacagc tccagctaca agtagtgtgt
     3481 aggatggtaa tttgtcatag gggagatttg gtttaagatg gagtgtcata taaataccac
     3541 cttgtggtga gtagaaggaa cgttgaaaac gtcctcggcc tgctgtttga taggaagcta
     3601 gatagagggt atttgcttca tggcctaaat caattgcttc ttttgcatct agttgtgttg
     3661 attttgtttc gggtttaaag ctgactttaa ttggaagatt ttcttctaga atctctggaa
     3721 gaataaggtc accattcatt agtttatatc ctctattttt gatactatca atttcaatgc
     3781 cttcttgttc tagtcgcttg atagctttcc aaattgatgt tcggcttagg gatagttttt
     3841 ctgcgatttt ttctccgctg atatagtcgg tttctttaga taggatttgg tagacagctt
     3901 ggtaggattt cataatgttg cctttctcac tagttggtat tgagagtatt cttttcttgt
     3961 atgacttgga gtctgattaa agtattgttt ataagctttc gaaaaatgga gtggatctga
     4021 aaaacctacc gagtatgcaa ttaccttgat ggactcttgg gtattttcga gaagttgtct
     4081 agctcggtgc attcgaacgt agagtaggta ttctttgggt gataaggtat taaattcttt
     4141 gaatacgctt gataagtagc ttctgtgaac ggatagttct tttgctaaat cttgaattgt
     4201 aagtgattga ggatagtggc tatcaattaa tcgtttgcat tcaagataga gttggtgggt
     4261 tgatgaaata ttcttttttt tctgattggg agcaatagtt cccagatgaa acatcagttc
     4321 atgaagttgt cccatgatat ggagttgagc taattcactt gattttgtaa tctgagcgaa
     4381 gcggacaatg tctgagatga gttttgcagt agtctgggta tgacaagttt cagattggat
     4441 gagataggat tgatcagaaa tttgagaaag agcaaaataa tcaggggatt tccctccagt
     4501 gattcctaac cagtagtagg cccaaggttc tttactatct gcttgataaa aggttagttc
     4561 ctctggtttt aatagaaaga aatctccttc ttttaaataa acaattttat ccttgtaatg
     4621 aaattttcct tgtcctttag taatgtaatg taggacgtat gtatcacgaa tggctggacc
     4681 aaaagagtaa ttaggtgtgc attcctcata tccataaaag cttagggcaa ggtcgattgt
     4741 tccagtctgg tattctgaaa aaactaacat gtgctacctc ctacctaaca ttttaccata
     4801 ttcttgagac atttttctat tttggaaagc gatttcaggt gataaaatat aatcaataaa
     4861 gtgatgaggt gaagtaaatg ggagttagga tagagaataa tctattttat gttgagagta
     4921 aaaatctaag tttgattatt gaaaatcgaa atggctactt acttttgaaa catttaggaa
     4981 agactattaa gaactataaa ggttccaata gtgtttatga acgagaccat gccttttcag
     5041 gaaatccaac ggctactaat cgaaccttta gtttagatac tcagcgacag atttttggac
     5101 aacatggctt aggagatttt aggaaaccaa ctatacaggt tcagcatagt gtaactgaag
     5161 taacagactt tcgatttgta gaagcaaaga ttttaaaagg tcagaatggt ccacagggct
     5221 taccttctcc acatagcatg gacgatacag agactcttgt cttaatgtta gaagattcta
     5281 aggctcaact tagtctgact ttgtattata ctacttttaa taatgatgcg actattacta
     5341 gctacagtaa attagataat aatagtaatc aggaagttgt catccataaa gatttttctt
     5401 ttatggctga ttttccagct gcagattatg aaatagcaac tctgcagggt gcttatgctc
     5461 gtgaaaagac tgttagacgt caacaggtag aacaaggaat cttttcgatt agttcaaacc
     5521 gaggtgcttc tggtcatgct caaacaccag ctcttctact atgcgaacaa ggagtcacag
     5581 aggatgctgg gaatgtgttt gctattcaac taatgtatag tggcaacttt gaagcttttg
     5641 ttcaaaaaaa tcaattgaat gaagttcggg tggctattgg cattaatcca gaaaactttt
     5701 cttggaagtt agcccctgag gaatactttg aaacaccggt agctttagtg actcattcag
     5761 atcagggatt aactggtatt agtcatgaaa gtcagaattt tgtactgaag cacattatgc
     5821 taagtgaatt ttctaaaaaa gaacgtccaa ttctaatcaa taactgggaa gctacttact
     5881 ttgactttca gagagaaaaa ctgttagagt tagcagatga agctaagaaa gttggcattg
     5941 aactttttgt attagatgat ggttggtttg gcaatcgttt tgatgataat cgtgctttag
     6001 gtgattgggt tgttaatgag gaaaaactgg gtggaagtct agaaagtctg atttcagcta
     6061 tccatgaaag aggtttgcag tttggacttt ggttagaacc cgaaatgatt tctgtagata
     6121 gtgatttgta tcgtcaacat cctgactggg ctattcaggt tcctgattat gagcatactt
     6181 attctcggaa tcaattagta cttaatcttg ccaatcctca ggtagtagaa tacttgaaaa
     6241 gtgtcttaga tcaactccta tcttatcatg agattgatta cattaaatgg gatatgaacc
     6301 gcaatatcac taagctaggg aatggattaa cttatctaga gacacagatg caatctcatc
     6361 agtacatgct ggggctttac gaactcgttt cttatctgac agagaagcac agccatattc
     6421 tctttgagtc ctgctctggt ggtggtggac gaaatgatct tggtatgata cgctatttcc
     6481 cacaagtctg ggctagtgat aatactgatg ccattgcacg tttaccaatt caatacggtt
     6541 catcctatct ctatccaacc atttctatgg gggttcatgt gtcagcagta ccgaatcatc
     6601 agatgggacg aatgacacca ttagaaacac gtggccatgt agcaatgatg ggaaatttgg
     6661 gctatgagct tgatttgaca aatttatcag atgaagagaa agctacgatt gctaatcagg
     6721 tgaacttgta taaagaatta cgaccagtag ttcagttagg acagcagtat agactaatta
     6781 atcctgatac tgcatccaat gaagcagctg tacaatttaa ttacggaaat caaacgattg
     6841 taacctacgt tcgcgtttta tctgttgtag agaccatgga aacaacttta aaattaaaag
     6901 atttggatga agagggacta tataaattac aggaaaatgg cgaagtttac tcaggtgcag
     6961 aactaatgta tgcgggctta actgttatct tatcccaagg agattttttg agtagacagt
     7021 atatttttag aaaactatga tatgtactga ccccaaaagt tagatttatt ctgtctaact
     7081 tttggggtgc agttcaataa gggtgtataa atttataaag gaggctttct aatggaatgg
     7141 tataaaaaaa tcggacttct tgcaactaca ggtttagctt tgtttgggct cggcgcttgc
     7201 tccaactatg gtaaatctgc ggatggcaca gtgaccatcg agtatttcaa ccagaaaaaa
     7261 gaaatgacca aaaccttgga agaaatcact cgtgattttg agaaggaaac cctaagatca
     7321 aggtcaaagt cgtcaatgta ccaaatgctg gtgaagtatt gaagacacgc gttctcgcag
     7381 gagatgtgcc tgatgtggtc aatatttacc cacagtccat cgaactgcaa gaatgggcaa
     7441 aagcaggtgt ttttgaagat ttgagcaaca aagactacct gaaacgcgtg aaaaatgcct
     7501 acgctgaaaa atatgctgta aacgaaaaag tttataacgt tccttttaca gctaatgctt
     7561 atggaattta ctacaacaaa gataaattcg aagaactggg cttgaaggtt cctgaaacct
     7621 gggatgaatt tgaacagtta gtcaaagata tcgttgctaa aggacaaaca tcatttggaa
     7681 ttgcaggtgc agatgcttgg acactcaatg gttacaatca attagccttt gcgacagcaa
     7741 caggtggagg aaaagaagca aatcaatacc ttcgttattc tcaaccaaat gccattaaat
     7801 tgtcggatcc gattatgaaa gatgatatca aggtcatgga catccttcgc atcaatggct
     7861 ctaagcaaaa gaactgggaa ggtgctggct ataccgatgt tatcggagcc ttcgcacgtg
     7921 gggatgtcct catgacacca aatgggtctt gggcgatcac agcgattaat gaacaaaaac
     7981 cgaactttaa gattggaacc ttcatgattc caggaaaaga aaaaggacaa agcttaaccg
     8041 ttggtgcggg agacttggca tggtctatct cagccaccac caaacatcca aaagaagcca
     8101 atgcctttgt ggaatatatg acccgtccag aagtcatgca aaaatactac gatgtggacg
     8161 gatctccaac agcgatcgaa ggggtcaaac aagcaggaga agattcaccg cttgctggta
     8221 tgaccgaata tgcctttacg gatcgtcact tggtctggtt gcaacaatac tggaccagtg
     8281 aagcagactt ccataccttg accatgaact atgtcttgac cggtgataaa caaggcatgg
     8341 tcaatgattt gaatgccttc tttaacccga tgaaagcgga tgtggattag gagtagagag
     8401 actatgaaaa aagtattaca aaaatattgg gcatgggctt ttgtggtcat ccccctcttg
     8461 ttacaagcaa ttttcttcta tgtgccgatg tttcaaggag ccttttacag ttttaccaac
     8521 tggacaggat tgacttacaa ctacaaattt gtcggcttaa acaactttaa gctcctcttc
     8581 atggatccaa aattcatgaa tgcgattggc tttaccgcaa tcattacgat tgccatggtg
     8641 gttggtgaga ttgcactcgg gatcttcatt gcgcgtgtct tgaactctaa aatcaaaggc
     8701 caaaccttct tccgtgcttg gttcttcttc ccagctgttt tatctggttt gacagtggct
     8761 ttgatcttca agcaagtctt caactacggt cttccagcga ttgggaatgc ccttcatatt
     8821 gaatttctcc aaaccagtct tttagggact aagtggggag caatctttgc ggctgtcttt
     8881 gtccttcttt ggcaaggggt ggctatgccc atcatcatct tcctagctgg tttgcaatct
     8941 attccaactg agattacaga ggcagcaagg attgatggtg cgactagcaa gcaagttttc
     9001 tggaacattg aattgcctta cttgctacca agtgtctcta tggtctttat cctagcccta
     9061 aaaggtgggc tgactgcctt tgaccaagtc tttgccatga ccggtggtgg tccaaacaat
     9121 gccacaacct cacttgggct cttggtttat aactatgcct ttaaaaacaa ccaattcggt
     9181 tatgccaatg ccattgccgt aatcttgttc ctcttaattg tagtgatttc gatcatccaa
     9241 ttgagagtat ctaagaaatt tgaaatttaa gaggagaagc atgatgaaac aagatgaaac
     9301 aagatgaaag aaaagccctg attggcaaat acattctatt gattctagga tcggttctga
     9361 ttttagtgcc gctccttgct accctcttta gttccttcaa acccactaag gatattgtag
     9421 ataatttctt tggctttcca accaacttca catgggacaa ctttagccgt ctcttagctg
     9481 atgggattgg aggctattat tggaactctg tcgtcatcac tgtcttgtct ttacttgcag
     9541 taatgatctt tatccctatg gcagcctact ccatcgctcg caatatgagt aaaagaaaag
     9601 tctttaccat catgtatacc ctcttaatcc tcggaatctt cgtacctttc caagtcatca
     9661 tgattccgat tacggttatg atgagtaaac tcggtttggc taataccttt ggtttgatct
     9721 tgctctactt gacctatgcg attccacaga ccctctttct ctatgttggc tatatcaaaa
     9781 tctcgattcc agaaagtctg gatgaagcag cagagatcga tggggctaat caatttacaa
     9841 cctatttccg catcatcttc ccaatgatga aaccgatgca tgcgacaacc atgatcatca
     9901 atgccctttg gttctggaat gacttcatgt tgccactcct tgtcttgaac cgggattcca
     9961 aaatgtggac tctgcctttg ttccaataca actacgcagg ccaatatttc aacgactacg
    10021 gaccaagctt tgcctcttac gtggtcggca ttatcagtat caccattgtc tatctcttct
    10081 tccaacgcca tatcatttca ggaatgagca acggggcagt gaagtaatac tcaatgaaaa
    10141 tcaaagagca aactaggaag ctagccgcag gttgctcaaa acactgtttt gaggttgtgg
    10201 atgaaactga cgaagtcagt aaccatacct acggtaaggt gaagctgacg tggtttgaag
    10261 agattttcga agagtataag aagagctcat ggaatctata gtctttgaca cagaggaaga
    10321 gttcagcttg ttgataattc tatgtagaaa acaagaaagt agtactttgt ttatcacagt
    10381 ctatattagt acgagtttga tgataaatag agatttaaaa tgaatttgat ttacgaagcc
    10441 agatcatatg agacttggct tcaattagaa aaaggagagt aatgatgccg attcaaaata
    10501 aaaccatgtt gattacctat tctgatagcc ttggaaataa tcttaaagac ttatatgata
    10561 atttggaaga gcattttgga gatgctattg gaggagttca ccttttacca tttttcccat
    10621 caacaggtga tcgtggattt gcgccagttg actacgacga agtggactca gcttttggtg
    10681 attgggagga tgttaagcgt ttaggtgaga aatattatct tatgtttgat tttatgatta
    10741 atcatatttc tcgtcaatcc aagtattata aggactatca agaaaaacat gaagccagtg
    10801 aatttaaagc tctcttttta aactgggata agttttggcc agaaaaccgt ccgacacagt
    10861 ctgatgtaga tttaatttac aagcgtaagg atcgtgcacc aaagcaagag attgtgtttg
    10921 aagatggttc agtggaacat ttgtggaata cctttggtga ggagcagatt gatcttgatg
    10981 tgaccaaaga agtaactatg gaatttatcc gtaagaccat tcagcacttg gcaagtaatg
    11041 ggtgtgattt gattcgtcta gatgcctttg cttatgcagt gaagaaattg gatactaatg
    11101 atttctttgt ggaaccagat atttgggatt tattggacaa agttcgagat atcgctgctg
    11161 agtatgggac agagctttta cctgagattc atgaacacta ttcgattcag tttaaaatag
    11221 cagaccatga ttactatgtt tatgattttg ctcttccaat ggtgacactt tatactcttt
    11281 acagttccag aacagagcgt ttggctaagt ggttaaagat gagcccgatg aagcaattta
    11341 cgacgctaga tacccatgat gggattggag tagtagatgt caaggatatc ctgaccgatg
    11401 aggagattga ctatgcttca aatgaactct ataaggttgg agccaatgtc aaacgtaagt
    11461 actctagtgc cgagtataac aacttagata tctaccaaat caattcaacc tactattcag
    11521 cgcttggaga tgatgatgtc aagtattttc tcgctcgtct aattcaagct tttgccccag
    11581 gtattcctca ggtttactat gtgggtctat tagcaggcaa gaatgacttg aaattattag
    11641 aagaaactaa agaaggtcga aatattaatc gtcattacta tagcaacgag gaaatagcaa
    11701 aagaagtgca acgacctgtt gtgaaggccc ttctcaatct attttctttc cgtaaccgtt
    11761 cagaagcctt tgatctagaa gggactactg agatagagac accaacagct cacagcattg
    11821 taatcaaacg tcaaaataaa gataagtccg taacagcagt agtagaaatt gatttgcaaa
    11881 atcagactta tcgggtaatt gagaatggag ttgaagtatc attttgaagc cttgatatgg
    11941 tggataaaat agggttttta ttttggaaaa cgtttccttt gttttcaaat tgctaaaaaa
    12001 gtggtacaat ataggatagc ttactattat ctgaatcagc tgatttggag agaaaggatt
    12061 cattttgaaa tcaataggct ttattgaaaa gctgaagggg ttgtctagta aagagctgat
    12121 tttattggga attatcctaa gtatcttttt acccttttat ctttttgtag ttgtactctg
    12181 tttatatatt atcagtttga tttttacagg agacatgaaa agtattcttc agaaaatggg
    12241 ggagcatccg atgctgcttc tttttcttag ctatagtact gttatatcca ttcttgcaca
    12301 aaattggatg ggtcttgtgg cttcagtagg aatgtttcta tttactattt tctttttgca
    12361 ctatcagtcg attttatccc ataaattctt tcgattgatt ttgcagttcg tcttgtttgg
    12421 tagtgtcttg tcagctgctt ttgccagttt agaacatttc caaattgtga agaaatttaa
    12481 ctatgctttt ctttcaccca atatgcaggt gtggcatcag aaccgggcag aagtgacctt
    12541 ctttaatcct aattattatg gaattatttg ttgtttctgt attatgattg ctttctatct
    12601 gtttacaacg accaagttga attggttgaa agtattctgt gtgattgcag gctttgtgaa
    12661 tctctttggt ttgaacttta ctcaaaatcg aactgccttt cctgctatta tcgctggagc
    12721 aattatctat ctctttacga ctattaaaaa ctggaaggcc ttttggctta gtattggggt
    12781 cttcgcgatt ggtttgagtt tcctcttttc tagtgatttg ggagttcgaa tgggtacttt
    12841 agactcttct atggaagaac gcatttctat ctgggatgct gggatggcct tgtttaagca
    12901 aaatcctttt tggggtgaag ggccattgac ctatatgcac tcttatcctc ggatacatgc
    12961 tccttatcat gaacatgccc acagtcttta tattgatacg attctgagtt acggaattgt
    13021 gggtaccatt ttattagttt tgtcttctgt tgctcctgtt cgcttgatga tggatatgag
    13081 tcaggagtcg gggaaacgtc cgattatcgg cctttatcta tctttcctta cagtggttgc
    13141 tgtgcacgga atttttgact tggctctctt ctggattcag tcaggcttta ttttcttgct
    13201 agttatgtgc agtattccat tggagcatcg aatgttggta tcggacatga cggattaagt
    13261 tttataagtt tgaaatcttc taccttgggt aggagatttt tttatacgat gggcatgtcg
    13321 tgcatctgag gtgtaagtcc tctgtaggga tccactaccg gtgaatacaa tagcgctccc
    13381 caagcctgac tatcgtgagg tagcggatta aaatggtctg gggatagatc gttttaagtc
    13441 tgacgcagga aataagaatt gtcagagaaa gcaatagtga aatcgtaggc tatgaataga
    13501 aacgtgatag cgaggagcat atagtggaca aggtagcgag aaattttaaa gtataaaaga
    13561 tagtcttaat ctatatgtgt aaatcacgaa agtaattgaa ttcggaataa ggttggtgtg
    13621 aaaagacata gtaaactgaa tcttgaatac tagaatgata cttctacaat attgttataa
    13681 tttaatttga attccaaaat cacattgttc cgtccgtatt tcattttact gtatgtcttc
    13741 ctaagctcca aaatttctgt gtcaaatttc ctattttcac tgtagtcttt gacacttcga
    13801 acgacaattt gaatcatata ttcaaaaaaa atgaaaaatt tgtgccaaca agcctaaata
    13861 ttctgaaaat ttgtttgatt tttaatggat aatgtgatat aatggttgtc aatctataat
    13921 tcacataaac agaggagtag aaaatgaaaa aaaatcgtgt atttgctaca gcaggtcttg
    13981 ttttattagc agcaggtgta cttgcagcat gcagttcttc aaaatcatct gattcatcag
    14041 cccctaaagc ttatggctat gtttatacag cagacccaga aaccttggac tacctgattt
    14101 caagtaaaaa tagtacaaca gtagtgactt caaatgggat tgatggttta ttcactaacg
    14161 ataattacgg taatcttgct cctgcagttg cagaggattg ggaagtctct aaggatggtt
    14221 tgacctacac ttataagatt cgtaaagggg ttaaatggtt tacctctgat ggagaagaat
    14281 atgcagaggt gacggctaaa gatttcgtga acggtttaaa acacgcagca gataaaaaat
    14341 cagaagctat gtatttagct gaaaattcgg ttaaaggctt ggcagattat ctatcaggaa
    14401 cttcaacaga tttttcaaca gttggtgtca aggcggttga tgattatacg ttacaataca
    14461 ctttgaacca gcctgaaccg ttctggaact ctaagttgac ctattctatt ttctggcctc
    14521 tgaatgaaga attcgaaaca tcaaaaggaa gcgattttgc taaaccaaca gatccgacat
    14581 ccttgcttta taatggtcca ttcttgttga aagggttgac tgcaaaatct tctgtagagt
    14641 ttgtaaaaaa tgagcaatat tgggataaag aaaatgtcca cctagatact atcaatctag
    14701 cttactatga tggatcagat caggagtcgc tagagcgtaa cttcactagt ggagcttata
    14761 gttatgcccg tctttaccct accagctcca actattctaa ggttgcagaa gaatacaagg
    14821 acaatatcta ttacacacaa tcaggctctg ggattgctgg tctgggtgtg aatattgatc
    14881 gccaaagtta caactatact tctaaaacta cagattcaga gaaagtagct actaagaagg
    14941 cattgcttaa caaagatttc cgtcaagcct tgaattttgc tcttgatcgc tcagcttact
    15001 cagctcaaat caatggtaaa gatggagcag ctttagcagt tcgtaattta tttgtaaaac
    15061 cagactttgt ttcagctggt gagaagacct ttggtgattt agtcgctgct caacttcctg
    15121 cttatggtga tgagtggaaa ggtgtgaatt tagctgatgg gcaggatggt ttattcaatg
    15181 ctgacaaggc caaggcagag tttgcgaaag ctaagaaagc tttagaagca gacggcgttc
    15241 agtttcctat tcatctggac gttccagtag accaagcatc aaaaaactat atatctcgta
    15301 ttcagtcctt taaacaatct gtagaaacag ttcttggtgt tgaaaatgtc gttgttgata
    15361 ttcaacaaat gacaagtgat gaattcctta atattactta ctatgctgcc aatgcttcat
    15421 ctgaggattg ggatgtatcg ggaggagttt catgggggcc agactatcaa gacccatcta
    15481 cttacctgga tattttaaaa acaactagca gtgaaactac aaaaacatat ttaggatttg
    15541 ataatccaaa tagcccttca gtagttcaag ttggtttgaa agaatacgat aaattagttg
    15601 atgaagctgc caaagagaca agcgacttga atgtccgtta tgaaaaatat gcagcggctc
    15661 aagcatggtt gacagatagt tcactcttta ttcctgctat ggcttcttct ggtgcagcac
    15721 cagtgctttc acgaattgtt ccatttactg gagcttctgc gcaaacaggc tctaaggggt
    15781 cagatgttta cttcaaatat ttgaaattac aagataaagc ggtgactaag gaagagtatg
    15841 aaaaagctcg tgaaaaatgg ttgaaagaaa aagctgaatc aaatgagaaa gctcaaaaag
    15901 aattggcaag tcatgtgaag taaataattt ctaatagaac tttctctcca tgaggagaag
    15961 gttcttttgg gatttttaaa ggaaataata tgaaaaaata tatttttatg cgtgttttgc
    16021 ggtcattggt ttcgattttc ttagtaacga ctttgaccta cacgattatc tataccttgg
    16081 ttcctcgaaa attgattttc aagcaggatc ctaactataa taaaattgcg acaacggctg
    16141 ataaacgtga taactatgaa aatactgtgt ttgagcgtat gggctacatt gagtattacg
    16201 atactaaaga gttgcaagaa aaggcaagta gcatggattc ttctgtaaca gtagaagcaa
    16261 atgcgaccaa taaagctatt tatgaaaagt acatcaatca attaggtcat ggttggactt
    16321 tgggagaatt tactgaaagt ggtcaattct atgctactcg tgaaattcca atttttgaac
    16381 gtgtttttca cttctatgct aacttgattg acattgacca tacaaataaa atccaagacc
    16441 ctgaaaatcc agacttgaaa cgctaccttc gttttgaaaa cgatccagct atcggatggt
    16501 cattggtcgg ttcaggaact aaacataaat atctcttgta ctttaacagt cagttcccat
    16561 ttgttcatca aaactttgtg aacttgaatt taggtgactc ttacccaacc tatgctaata
    16621 caccagttct tcaggttatt actcaaggtc aaggacaaac caaaactgcc caagttcagt
    16681 tcccaacagg taagaaaacg tcttctgtaa atatttactc aagaacctac aagtcaccta
    16741 gtcaggctga ctctcgtgaa gtagctagct atgggaaaga tgatccttat acagcgactg
    16801 aaagtaatta ccaatatcca tctatgattg tcagctctgc tattactggt ttgattggtt
    16861 tggttcttgc ctatgctctt gccgtgccac ttggttcagc catggctcgt ttcaagaaca
    16921 cttggattga tagcctctca acaggggctt tgaccttctt gcttgctctt ccaacgattg
    16981 ccttggttta catcgttcga ttgattggat catctatttc ccttccagat tcattcccta
    17041 tcttgggagc tggagattgg cgttcttacg ttttaccagc agtcatcctt ggtttgttgg
    17101 gtgctcctgg tacagccatt tggattcgtc gttacatgat tgacttgcaa tctcaagact
    17161 ttgttcgttt cgctcgtgca aaaggtttgt ctgaaaaaga aatttcaaac aaacacatct
    17221 ttaaaaatgc catggttccg ctggtttcag gaattcctgg tgccattatt ggggttatcg
    17281 gtggtgcaac ccttactgaa acagtcttcg ccttcccagg tatgggtaaa atgttgattg
    17341 actctgtaaa agcatctaat aactctatgg tcgttggtct tgtcttcatc tttacatgta
    17401 tttctatctt ctcacgtctt ttgggagata tttggatgac tattattgac ccacgtatta
    17461 aattgactga gaaaggaggc aaataatgtc tacaatcgat aaagaaaaat ttcagtttgt
    17521 aaaacgtgac gattttgcct ctgaaactat tgatgcgcca gcatattctt actggaaatc
    17581 agtgtttaaa caatttatga agaaaaaatc aactgtagtc atgttgggaa tcttggtagc
    17641 catcattttg ataagtttca tctacccaat gttttctaag tttgatttca atgatgtcag
    17701 caaggtaaac gactttagtg ttcgttatat caagccaaat gcggagcatt ggttcggtac
    17761 tgacagtaac ggtaaatcgc tctttgacgg tgtctggttc ggagctcgta actccatcct
    17821 catttctgtg attgcgacag tgattaactt ggttatcggt gtttttgtcg gtggtatttg
    17881 gggtatttca aaatcagttg accgtgtcat gatggaagtt tacaacgtca tctcaaacat
    17941 cccacctctt ttgattgtta ttgtcttgac ttactcaatc ggagctggat tctggaatct
    18001 gatttttgcc atgagcgtaa caacatggat tggtattgcc ttcatgatcc gtgtgcaaat
    18061 cttgcgctat cgtgacttgg aatacaactt ggcgtcacgt actttgggaa caccaacctt
    18121 gaagattgtt gccaaaaata tcatgcctca attggtatct gttattgtga caaccatgac
    18181 tcaaatgctt ccaagcttta tctcatacga agccttcttg tctttcttcg gtcttggatt
    18241 accgattaca gtgccaagtt tgggtcgttt gatttcggat tattcacaaa acgtaacaac
    18301 caatgcttac ttgttctgga ttccattgac aacccttgtc ttggtatcct tgtccctttt
    18361 cgtagttggt caaaacttag cggatgctag tgatccacgt acacatagat aggagtagaa
    18421 atgacaaaag aaaaaaatgt aattttgact gctcgcgata ttgtcgtgga atttgacgtt
    18481 cgtgacaaag tattgacagc cattcgcggc gtttcccttg aactagtcga aggagaagta
    18541 ttagccttgg taggtgagtc aggatcaggt aaatctgttt tgacaaagac cttcacaggt
    18601 atgctcgaag aaaatggtcg cattgcccaa ggtagtattg actaccgtgg tcaggacttg
    18661 acagctttat cttctcacaa ggattgggaa caaattcgtg gtgctaagat tgcgactatc
    18721 ttccaggacc caatgactag tttggacccc attaaaacaa ttggtagtca gattacagaa
    18781 gttattgtaa aacaccaagg aaaaacagct aaagaagcga aagaattggc cattgactac
    18841 atgaataagg ttggcattcc agacgcagat agacgtttta atgaataccc attccaatat
    18901 tctggaggaa tgcgtcaacg tatcgttatt gcgattgccc ttgcctgccg acctgatgtc
    18961 ttgatctgtg atgagccaac aactgccttg gatgtaacta ttcaagctca gattattgat
    19021 ttgctaaaat ctttacaaaa cgagtatcat ttcacaacaa tctttattac ccacgacctt
    19081 ggtgtggtgg caagtattgc ggataaggta gcggttatgt atgcaggaga aatcgttgag
    19141 tatggaacgg ttgaggaagt cttctatgac cctcgccatc catatacatg gagtctcttg
    19201 tctagcttgc ctcagcttgc tgatgataaa ggggaacttt actcaatccc aggaacacct
    19261 ccgtcacttt atactgacct gaaaggggat gcttttgctt tgcgttctga ctacgcaatg
    19321 cagattgact tcgaacaaaa agctcctcaa ttctcagtat caaagacaca ttgggctaaa
    19381 acttggcttc ttcatgagga tgctccaaaa gtagaaaaac cagctgtgat tgcaaatctc
    19441 catgataaga tccgtgaaaa aatgggattt gcccatctgg ctgactagga ggaaggaaat
    19501 gtctgaaaaa ttagtagaaa tcaaagattt agaaatttcc ttcggtgaag gaagtaagaa
    19561 gtttgtcgcg gttaaaaatg ctaacttctt tatcaacaag ggagaaactt tctcgcttgt
    19621 aggtgagtcc ggtagtggga aaacaactat tggtcgtgct atcatcggtc taaatgatac
    19681 aagtaatgga gatatcattt ttgatggtca aaagattaat ggtaagaaat cgcgtgaaca
    19741 agctgcggaa ttgattcgtc gaatccagat gattttccaa gaccctgccg caagtttgaa
    19801 tgaacgtgcg actgttgatt atattatttc tgaaggtctt tacaatcacc gtttatttaa
    19861 ggatgaagaa gaacgtaaag agaaagttca aaatattatc cgtgaagtag gtcttcttgc
    19921 tgagcacttg actcgttacc ctcatgaatt ctcaggcggt caacgtcaac gtatcggtat
    19981 tgcccgtgcc ttggtcatgc aaccagactt tgttattgca gatgagccaa tttcagcctt
    20041 ggacgtttct gtacgtgccc aagtcttgaa cttgctcaaa aaattccaaa aagagctcgg
    20101 cctgacctat ctcttcatcg cccatgactt gtcggttgtt cgctttattt cagatcgtat
    20161 cgcagttatt tacaagggtg ttattgtaga ggttgcagaa acagaagaat tgtttaacaa
    20221 tccaattcac ccatatactc aagccttgct ttcagcggta ccaatcccag atccaatctt
    20281 ggaacgtaag aaggtcttga aggtttacga cccaagtcaa cacgactatg agactgataa
    20341 gccatctatg gtagaaatcc gtccaggtca ctatgtttgg gcgaaccaag ccgaattggc
    20401 acgttatcaa aaaggactaa actagtaatg gttttataat ttccatgtca atttttatgg
    20461 aaattataaa actttttttg ttggactgat ttgaaaaact ttgaaagctt cctgaaagaa
    20521 tttttgaaaa attttaaaaa ttatgaaatg ttattgacag gtgatgaaaa aggtggtagg
    20581 acaggaagca tcaaaaaaga aagcagaaaa agaaatgaat aagagacaag ccgtaactat
    20641 gaatcaaaac ttttacttta gctactttag atagtgtttg tagacatgtc accatggatg
    20701 caaacagttc aagcaatttg tttgtatcta tgtggctaag atttttgatt gtggtccata
    20761 tagatgaata tctaaatgga ctagctaagt tgtaacttgt taggttattt caagtatccg
    20821 tttagatagt atctaggcgg ttttttgttt tatcttttct gagaaggagt ttcattatga
    20881 aagttgttcg aaaatcacta gccccctctt ggtagtgggg ctcctcttga cttctctgat
    20941 cagtttgcat cagttgaagg cagataagaa aaagatgtct ttcgtatcgg tatttcgcaa
    21001 tttattaccc accagtcctt atacgctact agagaaggat ttgtggatga gctggccaaa
    21061 caaggctatg ttgaggggga aaatattgag attgatttgc aaaatgcaca gggagaacaa
    21121 agaaatctaa aaacgatttc ccagcaacta gcagaatcca gcgatgtcgt tctagccatc
    21181 gcaaggcctt ctgctcagag cttggccaat acgacgcaaa cgacaccagt tatcttttca
    21241 gctgtaacag acccagtcag tgccaagttg gttgagtcaa gagaacatcc tgggggcaat
    21301 gtaactggga caagcgatca gtcatcagat gccatttcaa cccaaatcaa tttgataaag
    21361 aaagtgttgc caaaggctaa aacgattgga atcctctata ctcagagcga gcccaattcg
    21421 gttgttcaaa aggatgaagc taagcgactt ctgaaagaaa aaggctttac cgttgttgaa
    21481 aaaacaatct tggacagtaa taacgtcaag gcggcagcag agagtttgat ggcagaggtg
    21541 gatatggttt ttgtcccaac tgataatatc atttcgttaa ccatggaaac agttaagcag
    21601 gtttctatta aacacaaggt tccagtattc ggtggttcaa cagaaatgat tgctgttggt
    21661 ggcttgtata actacggaac caattatgaa gaattggtac gtcagacagc tcgcatgctg
    21721 attcgtgttt taaaaggtga gaaaccagaa aatatagcag ttgagttgcc tgaaaaactg
    21781 gaattgcata ccaatcaaga aatggcagat gcattgggaa ttgatattag taagttagaa
    21841 ggcaaggaat aaggaggatt aagatgaatt ttgtgttatc tagtttatca gaaggtttgt
    21901 tgtggtcgat tgtggcgatt ggggtctact tgactttccg tattttggat attgcggata
    21961 tgactgctga gggagccttt cctctggggg cagcagtcgt tgtatcacag atacaggcag
    22021 gggcaaatcc ttggcttgcg actttacttg ctttgctggc aggtatggta gcaggtcttg
    22081 tatcaggaat gctccacacc aagatgaaaa tcccagctct cttgacaggg attgtgacct
    22141 tgacagggct ctattcaatc aatattaaaa tcatgggcag tgtacccaat ctttccttgg
    22201 gagattctgc aaccgtcttt aaacagttgg cgagcttggg tctgacaaat gaaggaactg
    22261 ttttctcact tagtttggcc tgttttttac ttgtttgttt ggtcttgacc cttttgatga
    22321 aaacagagat tggcttggtc ttgcgttcga ctggggataa tatcccgatg agtgaggcca
    22381 atggagtcaa tgtagacacc atgaagattg ttggttacat gatttcaaac ggtttgattg
    22441 ctctatgtgg ttccttgttt gcccaaaatg atggattttc agatgtgact tctgggacag
    22501 gaaccattgt tgttggtttg agctcggtga ttattgcgga agtcttgata cacgacttga
    22561 ccattggagg ccgcttatta tccatcggaa tcggtgctat tgtttaccgt ttgattattt
    22621 taaatatcta tgaaattcca aatctagatc aaaacttggt acgtctcttt aatgcaatct
    22681 tgctcgcttt ggttttattt gcaccagaat tgcaaaagag attaaagatt cgtggtctga
    22741 aattgagaaa tgaataggag gagaaagtta tgacaagttt gttaacactt gaaaatattc
    22801 acaagacatt tgaagcaggg acggtcaatg agaaccatgt cctcaaagga ttagatttag
    22861 aggttgaaga gggagatttt atctctgtga ttggtggaaa tggagcagga aaatccactt
    22921 tgatgaatat cttggctggc aatctatcgg tggacgaagg ggaccttcta ctggcaggga
    22981 aatccattaa aaatctgagt gtcagaaaga gagccaagga tattgcccgt gtttttcaag
    23041 atcctaagat gggaacagct tctcgtttga cgattgagga gaatatggct attgccttga
    23101 gacgcgggca gaagagaggg cttggttggg gcgtgaagga gaaggataga atccagttcc
    23161 aagaggcctt gaaggagttg aatattggtc ttgaaaaccg cttaaaagta gatactcaat
    23221 atctctcagg aggacaaaga caggctttga ctctagtcat gacagatctg atgaaaccaa
    23281 aacttttact tttggatgaa catactgcgg cacttgaccc gaaaaccagt caaatggtga
    23341 tggacttgac gcaaaagatt gtggagcacc atcagtggac gactttgatg attacccatg
    23401 atatgaatca tgcgattgag tacggcaatc gtctcattat gctctatcaa ggcaagatag
    23461 tggtggatgt caagggagaa gagaaaaagc atctgacagt tgaagacctt atgcatctct
    23521 tccagaaaaa tagtggccaa agtctggtca gtgatgaatt agttttggga taaagaaaaa
    23581 ggctgagatc tggtgtctca gtcttttatt tcttgcgttt tgcaaaatct acaaatctaa
    23641 atttctctaa cttatggcga ctttcggtaa actgaaattg gcgtccgtct tggagataga
    23701 cttttgattt aatcgaaacg acataagggt ctttgccaat gtccatgaga atcttgtctc
    23761 ggtcgcttga gtggtcaatg gtgatttcct tctgagcata atcaataagg agtttgaggc
    23821 cattttctat ataagaatag atagactgct cagcaatttg gcgagtgaga tttggaatta
    23881 gttccatatc cagatagtcc gtatccagaa cggataccag atcatccacc acacgctggc
    23941 ggaccacctt ccaaaccatc cgaaactctg ggaaaccggt tatcagtgag gattttttat
    24001 caataataat cttgtccaga ctgaccacgt tggttttaga gcgcagtcca agttctttaa
    24061 ctagttcttg gtagctggtt aggttggata cagggaaatt gacggtttct tct
//
LOCUS       DAVKLX010000032        23827 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKLX010000032 DAVKLX010000000
VERSION     DAVKLX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.032"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(18..3194)
                     /gene="carB"
                     /locus_tag="VW325_001380"
     CDS             complement(18..3194)
                     /gene="carB"
                     /locus_tag="VW325_001380"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9734760.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(3506..4585)
                     /gene="carA"
                     /locus_tag="VW325_001381"
     CDS             complement(3506..4585)
                     /gene="carA"
                     /locus_tag="VW325_001381"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9734761.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(4635..5558)
                     /locus_tag="VW325_001382"
     CDS             complement(4635..5558)
                     /locus_tag="VW325_001382"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9734762.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(5577..6098)
                     /gene="pyrR"
                     /locus_tag="VW325_001383"
     CDS             complement(5577..6098)
                     /gene="pyrR"
                     /locus_tag="VW325_001383"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9734763.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(6309..6938)
                     /gene="nth"
                     /locus_tag="VW325_001384"
     CDS             complement(6309..6938)
                     /gene="nth"
                     /locus_tag="VW325_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9734764.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(6938..7480)
                     /locus_tag="VW325_001385"
     CDS             complement(6938..7480)
                     /locus_tag="VW325_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9734765.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            7649..7789
                     /locus_tag="VW325_001386"
     CDS             7649..7789
                     /locus_tag="VW325_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blpT protein"
                     /protein_id="HEU9734766.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQVTADDIRLQELLNTTMQELKKQ
                     KS"
     gene            8204..9763
                     /locus_tag="VW325_001387"
     CDS             8204..9763
                     /locus_tag="VW325_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9734767.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(9912..10811)
                     /gene="htpX"
                     /locus_tag="VW325_001388"
     CDS             complement(9912..10811)
                     /gene="htpX"
                     /locus_tag="VW325_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9734768.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKHM"
     gene            complement(10813..11373)
                     /locus_tag="VW325_001389"
     CDS             complement(10813..11373)
                     /locus_tag="VW325_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9734769.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            11467..12180
                     /gene="rsmG"
                     /locus_tag="VW325_001390"
     CDS             11467..12180
                     /gene="rsmG"
                     /locus_tag="VW325_001390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9734770.1"
                     /translation="MKPETFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12483..13766
                     /locus_tag="VW325_001391"
     CDS             12483..13766
                     /locus_tag="VW325_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9734771.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFIPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(13961..15532)
                     /gene="ffh"
                     /locus_tag="VW325_001392"
     CDS             complement(13961..15532)
                     /gene="ffh"
                     /locus_tag="VW325_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9734772.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15544..15876)
                     /locus_tag="VW325_001393"
     CDS             complement(15544..15876)
                     /locus_tag="VW325_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9734773.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(15967..16344)
                     /locus_tag="VW325_001394"
     CDS             complement(15967..16344)
                     /locus_tag="VW325_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9734774.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            16416..17720
                     /locus_tag="VW325_001395"
     CDS             16416..17720
                     /locus_tag="VW325_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9734775.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESQNPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            17733..18539
                     /gene="yidA"
                     /locus_tag="VW325_001396"
     CDS             17733..18539
                     /gene="yidA"
                     /locus_tag="VW325_001396"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9734776.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLTKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(18594..18665)
                     /locus_tag="VW325_001397"
     tRNA            complement(18594..18665)
                     /locus_tag="VW325_001397"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18630..18632),aa:Arg,seq:cct)
     gene            complement(18707..19054)
                     /gene="rplS"
                     /locus_tag="VW325_001398"
     CDS             complement(18707..19054)
                     /gene="rplS"
                     /locus_tag="VW325_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9734777.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(19173..19502)
                     /gene="crcB"
                     /locus_tag="VW325_001399"
     CDS             complement(19173..19502)
                     /gene="crcB"
                     /locus_tag="VW325_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9734778.1"
                     /translation="MVIVYLAIACGFGALVRYLFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(19496..19870)
                     /gene="crcB"
                     /locus_tag="VW325_001400"
     CDS             complement(19496..19870)
                     /gene="crcB"
                     /locus_tag="VW325_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9734779.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(19867..20133)
                     /locus_tag="VW325_001401"
     CDS             complement(19867..20133)
                     /locus_tag="VW325_001401"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9734780.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVGSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(20249..20692)
                     /locus_tag="VW325_001402"
     CDS             complement(20249..20692)
                     /locus_tag="VW325_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9734781.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            20796..21731
                     /locus_tag="VW325_001403"
     CDS             20796..21731
                     /locus_tag="VW325_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9734782.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            21785..22069
                     /locus_tag="VW325_001404"
     CDS             21785..22069
                     /locus_tag="VW325_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9734783.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(22126..22704)
                     /locus_tag="VW325_001405"
     CDS             complement(22126..22704)
                     /locus_tag="VW325_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HEU9734784.1"
                     /translation="MVRNYWKAKSWLLVLALSFLILSPAGAQESLSFFFVKITDASKT
                     VKNGGQTETQKLVTKMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVV
                     RDNSTAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(22679..23692)
                     /gene="efeB"
                     /locus_tag="VW325_001406"
                     /pseudo
     CDS             complement(22679..23692)
                     /gene="efeB"
                     /locus_tag="VW325_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
     gene            complement(23695..23827)
                     /locus_tag="VW325_001407"
                     /pseudo
     CDS             complement(23695..23827)
                     /locus_tag="VW325_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         6997 a         5367 c         4561 g         6902 t
ORIGIN      
        1 atatcttatt tttctattta aatcgcttct gtaacaaagc tacgactttc aagtacctta
       61 agcatggcat tagctgtatc tagcgctgtg aagaggggca ccccgtgttc aatggctgaa
      121 cgacgaattt gctcaccatc ttcgtcagca gttcgttttg ttcctactgt gttaatgata
      181 gcttgaattc ttcctttgcg tacaaaactt gggatatcct tatcgtcatc accaatctta
      241 ccaacaggtt gggcttgcaa gccatgacta gcaaagaagg ctgctgtccc ttctgtcgca
      301 aggattccat aaccaatgtt ttggaaacga cgagccaagt tcaaggcttc ttctttggca
      361 tcatcagcga tggtaaagac gacattacca aaagttggca agtgtagata agaagcttca
      421 aaggctttat agagagcttt ttccaaagta gcatcagaac ccataacttc acctgttgac
      481 ttcatttcag gaccgagcaa gctgtctacc ttagctagtt tggtaaagga gaagacaggt
      541 gccttgatat gaacacgggt gctttcaggg taaagtccat tttggtagcc aagttctgat
      601 aaactttgac caagaatgag cttggtcgct acttgagcca taggaatatt ggttacctta
      661 gataggaatg gaacagtacg gctggcacgt ggattgacct caataacgta gactttttca
      721 tccttgataa caaactggat gttcatcatt ccaaggcagt gaagaccgat tgctaagcgt
      781 ttggtgtagt ctgcgatggt ctcctgaacc ttttgcgaca aggtttgtgg tgggtaaaca
      841 gccattgagt cacctgagtg gacaccagca cgttcgatat gctccatgat accaggaatg
      901 agcacatctt taccatctga aatggcatca acttcgcact cttgcccaac gatataagag
      961 tcgacaagaa ctgggtgatc tggactagcc ttaacagcag ttcgcatgta agaacgaagg
     1021 tcttcttcgt tttcaacgat ttccatggca cgtccaccaa gtacataaga tgggcggaca
     1081 agaactggga agccaatctt acgagctgca agagctgctt cttcttcatt ggtagccgtt
     1141 tgtcctggtg gctgtggaat atccaattct ttgagagctt gctcgaagag gtcacggtct
     1201 tcggcacggt ctaggtcagc aacctgtgta ccaaggatgg tcacacctgc ttttgccaat
     1261 ggctccgcaa ggttgatggc tgtttgacca ccaaactgaa cgataactcc ctttggttgt
     1321 tccaagtcaa tgacgttcat aacatcttcg aatgtcaatg gctcaaagta aagcttatct
     1381 gatacagaga agtctgttga aacggtctct gggtttgagt tcatgatgat agcttcataa
     1441 ccagctgcct ggatagcctt aacagagtga acggttgcgt agtcaaactc aaccccttga
     1501 ccgatacgga ttggacctga acctaggaca agtacagatt ctttatcaga tctgatagat
     1561 tcattttccc aaccataggt tgaatagaaa tatggcgttt cagagtcgaa ctctgccgca
     1621 caagtatcta ccatcttata aactggaaca atcttgtttt ccaagcgaag ttggcgaact
     1681 tgatcatcag tcgttcccca gagttcagca atcttacggt ctgagaaacc attaagtttg
     1741 gctgttttca aaacttctag atcttgtgga tgagcaccca attcttgctc aatttcaaag
     1801 atatgcaaga gtttatcaag atagaagata tcaatttttg taagctctgc aatttcttca
     1861 ggtgtgtagc cacgacgaat ggcttctgat acgtagaaga gacggtcatc ttgggctttg
     1921 acaacctttt caatcaaggc atcatcagaa actgctgcaa gttcaggtat ttcattgtgg
     1981 tgcaccccaa tttcaaggga gcggcaggcc ttgagaagag attcctcgat gttacgaccg
     2041 attgccatga cttctccagt cgccttcatt tgtgtaccga gacggcgttc acccttttca
     2101 aacttgtcaa atgggaaacg tggaatctta gcaactacgt agtcaagggc tggttcaaac
     2161 atggcatagg ttgaacctgt aactgggttt ataacctcat ccaaggtcaa acctactgca
     2221 atcttggcag ccaacttagc aatcggatat cctgtcgctt tagaagcaag ggctgacgaa
     2281 cgtgatacac gagggtttac ttcgataaca taatacttga agctgttagg atcaagagct
     2341 agctgaacat tacatccacc ttcaatcttg agggcgcgaa taatgctcaa gctcgcatca
     2401 cgaagcattt ggttttcata gtctgacatg gtttgcgcag gggcaaatac aatggaatcc
     2461 cctgtgtgaa tcccaactgg gtcaaagttt tccatgttac aaacaaccaa ggcattgtca
     2521 gctgagtcac gcatcacttc gtattcaatt tccttaaaac cggcaatcga acgctcaatc
     2581 aaacattggg taacaggtga caatttcaaa ccattttcag tgatttcacg caattctttt
     2641 tcgttggcac acataccacc accagtacca ccaagggtaa aggctggacg aacgatgact
     2701 gggtagccaa ttgtcgctgc aaaggcaact gcttcttcta ctgtgttaac aatttcagat
     2761 tctggaatgg gttgctcaag ctcttccatc aattgtttaa agaggtcacg gtcctccgct
     2821 tggtcaatgg cagataattt ggtacccaga agttcaacgc caagctcgtc taggatacca
     2881 tttttagata attccatggc catgttgaga cctgtctgac caccgagtgt tggtagcaag
     2941 gcatctggac gttccttacg aagaatacgt gtcacaaact caagtgtaat cggttcaatg
     3001 taaaccttgt cagcaatttc cttgtccgtc atgatggttg caggatttga gttaaccaaa
     3061 acaacctcat aaccttcctc tttcaacgac aagcaagcct gggtcccagc gtagtcaaac
     3121 tcagcagcct gaccaataat aatcggacca gaaccaatca ccataatttt ttgaatatca
     3181 gtacgtttag gcatagttta tgatacaaag cccgtaaaga acacagtgaa aataggaaac
     3241 tcggcgcaga cgccttcagc gtcaagacga tgtttatctt tttcacacag ttcttagggc
     3301 gtgttcactt ccgcgaacaa tgtatcttct cctttctatt cgtcgcctct cagggcgaca
     3361 ttaaataaga tacaaaggac gaatagaaag cgattgaatt ttaagaaatc aaggaaggat
     3421 tgacaatcca agttggtttc tctaaattct gagctttccg tccgtgttca gttacataaa
     3481 ttctccgacg agcttttact cgttcttagt ttgattgttt aaaaacttcc atcatctcga
     3541 taaactcgtc aaataggtag ctagcgtcgt gtggcccagg agctgcatct gggtggtatt
     3601 gaacagagaa agcaggttgg tatctgtggc gcacaccttc cactgacttg tcattgattt
     3661 cttcgtgggt aataatcaag tgctctggca aatcctcgcg gctgactgca taaccatggt
     3721 tctggctggt gaagtctact cgtcctgttg cgatttcacg taccgcatgg ttgaatccac
     3781 gatggccaaa cttcatctta taggtcttag ccccgtttgc cattgcaaag agttggtgtc
     3841 ccatacaaat accaaagatt ggaatttttc cttgtacacc gcgaatcatg tcgagtgctt
     3901 gtggaacgtc ttctgggtta cctggaccat ttgacaacat aactccatca ggattgagat
     3961 ggagaatttc ttcagccgtt gtcgaataag gaacaactgt cacgttacag ttgcgtttag
     4021 aaagttcacg taggattgag tgcttgagac caaagtccac tagcaccacg ctcaaaccaa
     4081 ctcctggagc tggataagac gttttagtag aaacctgttt gatattgtct gtcggtaaaa
     4141 ctgttgcttg gagctggtcc gtcacatggt ccatactgtc cccaacatgg gtcaaggttg
     4201 cacgcatagt accatgctta cggataatct tggtaagagc acgcgtatca attcctgaaa
     4261 tccctggaat tttcttggct ttcaaaaatt catccaaggt catttggttg cgccagttgc
     4321 tagctctacg cgcttcttca aaaacaacga ctcccttaca agttggaata atggattcat
     4381 aatcatcacg attaatacca taatttccta ccaaaggata agtaaaggtc aagatttgtc
     4441 cattataaga ctggtctgta atggattctt ggtagccggt catccctgta ttaaagacga
     4501 tttcgcctgt tacatcaata tctgctccga aggccttgcc ttcaaaaact gtgccatctt
     4561 ctaatactag aattcttttt gtcatatttt cacctctcgt ggacgctcac tggcgtcttt
     4621 taacgtcttg tgttttagtt ggcgtttcta ctcgctagta cggattctaa gattgccatt
     4681 cgaacaaaga caccattggt catttgttgg acaatccgtg attttggtgc ttcaaccaag
     4741 tggtctgcta tttctacatc acgattgatt ggagctgggt gcatgaggat tgctgtttct
     4801 ttcaaacgat cgtaacgttc ttgagtcaag ccatgttggg catggtagtc ttcttttgaa
     4861 aatacagctc cactatcatg gcgttcgtgt tgcacacgga gaaacatcat gacatcaacc
     4921 tgatcaatga tttcatcaat ggttacaaac tgtccatagt ctgcaaactc ttgacttctc
     4981 cattcctcag gtccagcgaa aaagagttca gctcccaagc gtttcaaaat ctgcatattg
     5041 gatttggcaa cgcgtgagtg atccaagtca cctgcaatag caactttaag accctcaaag
     5101 tggccaaatt cctcataaat ggtcatcaaa tcaagcaagc tctggctagg gtgttggccc
     5161 gaaccatctc caccattgat gatggaagtc gtaatcgttg gactagcaat caattctcta
     5221 tagtagtcga cctctggatg gcgaatcaca cagacatcca ctcctaaagc agacagagtc
     5281 aaaatggtgt cataaagtgt ctcaccctta ttaaccgaac tagtcttcac atcaaagtca
     5341 agtcgttcca atccaagttt aatctctgcg acttcaaagg acttatgtgt ccgtgtagaa
     5401 tcctcaaaga agagattgga aacaatcgga tggtcttcat agggaagctg ggctccattt
     5461 ttaaactcaa ttcctcgctt gatcaatttc attacttgat cgacagtgag gtcttccatg
     5521 gacaccacat ggttcaatgc ttgttgattt tctgacatgg ctactccttt aactttctaa
     5581 gcttcttcag taatcagaac tctgtcttgg tcatcaagtt ctgtcatctc tacgatgatt
     5641 tcttcagaac gactggttgg gatatttttt ccaacgtaat ctggacggat tggcaattct
     5701 ctatgtccac gatcgactag aactgctaaa ctcacacgcg caggacgacc atgaccgaca
     5761 atattatcaa tagcagcacg gatggtacga cctgtataga gcacatcatc caccaagata
     5821 acttcgcggt ctgtcacatc gacagaaacc aaagaagtat cttctccact tttaacatca
     5881 tcacggaaag gtttagtatc caattccaca acaggaactg aaagattttc taactgcttc
     5941 aaacgttctt ggattcggtg ggcaataaag acaccacgag ttttaatacc agccaagacg
     6001 atcttattca aatctttgtt gcgttcgata atctcataag taatacgcgt aatcgctcgt
     6061 ttgacggtca attcgtctac aacttctttt gttttcatga caaacctcca aaaagaaaag
     6121 tctccttaaa caaggagact tgaaatttat agccaagcga gccctactgc acacagtgta
     6181 gacttcaccc ttctacttta tcgcgctcct tgcctgcctc acgggacagg tttaaaggaa
     6241 tatttagtta tcatttacta tagcacaaag catgcttaaa atcaagcaaa aagtttcaat
     6301 gtagcatctt acaaattgct aaaatcatat aattgtgggt actggtcaca ctctggattt
     6361 tttggatggc aaatggctct tccaaaataa atcatggcct gatgggcagc taaccactgc
     6421 tcaggcggca agatatccat gacccgcttt tccacctcaa gtggcgtcgc tgattttttg
     6481 acaatatcgt ggtgtttgca aatacgctcc acatgagtat ccactgcaaa ggctggaatc
     6541 ccaaatccta cactcatgac aacattggct gtcttgcgac caacacctgc caaactctcc
     6601 aattcttcac gtgtctgagg gacttgacca tcaaaatcgt ctagtaactg ttgggcacat
     6661 tttttaagga atttagcttt attccgatac aatcccaagc gagaaatatg tgaagcaatc
     6721 tcactctctg tcgctacaga catagcttgg ggtgttggaa aggcaacaaa gagacctggt
     6781 gtggccttat ttaccgctgc atctgtcgtc tgggctgata acatgaccgc aaccaggagt
     6841 tcaaaatgat tggtaaaatc aagactaggc ttggcatctg ggaagagggc aatgatttct
     6901 tctagcacct ttcgtgctcg tttttttgac aagaccatta ttcatctccg tcaaatagtc
     6961 cttgtaagcc agcaaaagga ctgttttctt ctttctttac tgcttgttga gcttggtatt
     7021 cttcctctgt catgatttgc cagtcatttc ctgagacaaa tccttgacca gcttcttctt
     7081 cagccgtcaa gaccttgata ggaatgttta gcaggatatt gtctgataca ctctcagcaa
     7141 ggtcaagcgc cccattttcg atgggcaaga ccaagtcatc atctaaaact tcttgatcta
     7201 gctggttagt tgcgccttcc atgaaaactt ccgtgactgg ataagattca actaactcaa
     7261 ctggctccat actgcgactc gaagcaagaa caatggtata agatagttga taatctaaga
     7321 aatacatacg gtcttcatat tgtactttcc caactgcaag gatatctttt acatctaaaa
     7381 tttcttgatt acgtgcacgc aggtcatcaa ctaaatctaa cgtttgttca aagttcaaac
     7441 cttcagactg cttacgaatt tcttgaatat ttaatttcat acttcctcca taaagattta
     7501 ctctcttgat tataccatga aaaggctaca aatcagcaca ccaaactttg taattaaaat
     7561 tcaaatttta acatatttac tatgatagtt ttatttttta gtgctatact atagggaaag
     7621 agtacatcag atcaaggagg atgctcacat ggaagacaag aaactcattc aactcctatc
     7681 caagttaaat aaaagctacc aaaactgtaa acaggttacg gcagatgata ttcgactaca
     7741 agagctgcta aacactacta tgcaagagct caaaaaacag aaaagttgaa caacagtatc
     7801 ttaattaatc ttgagaaatt ttaccaaccc accagtcctc tgattggact gggtagccta
     7861 aaactaaaca atcaagcacg cactgcttgg cgaaactatg ataaaatcca ttacgagtat
     7921 gtcaaacacg tactaagtct ctatggacct gtttttggat tttagagcat agaatttcca
     7981 gttttctgtt gacaaaattt ccttaaaggt ataatataaa gatactaata ctcggaggta
     8041 agggagacat gaacaactaa gtctatcaaa taaagaacct ttatttagta gatcttgttt
     8101 ttgtctcttt ttgtgtgctc ttttatgctc tttttctggc atgttaatag agtttttttg
     8161 acatagactt tgggctctac taggtaaagt agagcttttt gttatgcact atcgacattc
     8221 tagaaagggc aatagtatga taaaaatcaa tcacctgacc atcacacaaa acaaagattt
     8281 acgagacctt gtctctgacc taagcatgac catacaagac ggggaaaagg tagctattat
     8341 tggcgaagaa ggaaatggta aatcaacctt acttaaaact ttaatggggg aagatttgtc
     8401 tgatttcact atcaagggag acatccaatc tgactatcag tcactggcct acattcctca
     8461 aaaacttccc gaggagctga aaaagaaaac tttacacgac tacttctttt tagattctat
     8521 tgatttagac tacagtatcc tctatcgttt ggcggaggaa ttgcattttg atagcaatcg
     8581 tttcgcaagt gaccaagaga ttggcaatct atcaggggga gaagctttga aaattcagct
     8641 tatccatgag ttagccaaac cctttgagat tctattttta gatgaacctt caaatgacct
     8701 agaccttgag acagttgatt ggctaaaagg ccagattcaa aagaccaggc aaaccgttat
     8761 tttcatttcc catgatgaag actttctttc tgaaacggca gacactattg ttcacttgcg
     8821 actggtcaaa caccgtaaag aagcggaaac gctagtagag catttagact atgatagcta
     8881 tagtgagcag agaaaggcta attttgccaa acaaagtcag caagctgcta acaaccaaag
     8941 agcctacgat aaaaccatgg aaaaacatcg gagagttaag caaaatgtag aaactgcgct
     9001 tcgagctacc aaagatagta ctgccggtcg cctattggct aaaaagatga aaactgtcct
     9061 ctcacaagaa aaacgctacg aaaaggcagc tcagtccatg actcaaaaac cacttgaaga
     9121 ggaacaaatc caacttttct tttcagacat ccaaccatta ccagcttcta aagtcttagt
     9181 ccaactggaa aaagaaaatt tgtccattga cgaccgagtt ttggttcaaa aactacaact
     9241 aactgtccgt ggccaagaaa aaatcggtat tatcgggcca aatggtgttg ggaaatcaac
     9301 tctgttagcc aagttacaga gacttctgaa tgataaaaga gagatttcac ttggttttat
     9361 gccacaagat taccacaaaa aactgcaatt ggatttatcc ccaatagcct atctcagtaa
     9421 aactggggaa aaagaggaac tacagaaaat ccaatctcac ctagctagtc tcaatttcag
     9481 ttatccagaa atgcagcatc aaattcgctc cttatctggc ggacaacagg gaaaactcct
     9541 gcttttggat ttagtcctgc gcaaaccaaa ctttctcctg ctggatgaac ccacacgaaa
     9601 cttttctccc acttctcaac cccaaatcag aaaactcttt gccacttatc ctggcggtct
     9661 catcactgtt tcgcatgacc gtcgtttctt aaaagaagtc tgctcgatca tctatcgcat
     9721 gacagaacac ggtttgaagc tagttaattt agaagattta taaatttgca acatagcaga
     9781 taggtggggc aaaaacacaa ttttaggaga aaaagaagta aatcttccca tagtaaaacg
     9841 cataatatca agttttcaca cctgatatta tgcgtttttc agattttaaa gacttttttc
     9901 cagccttcat tttacatatg ttttaaacgt tcaatccgtt ctgagatagg tgggtgggta
     9961 taaaagagtt tttggaaccc cccacctttc ttgggatcat tgatataaag ggcactgcta
    10021 gcatcatcga cgtggcgact cataggtttg ctattgtcca acttatctag ggcattaatc
    10081 attccctggg gattgcgagt cagctcgaca ctagatgcat ctgccagaaa ttccctctga
    10141 cgagaaatag cgagctgaac caaggttgca gcgagaggtg ccagtacaat agctagtagg
    10201 gaaaccacta gcataatgat ttcaagacca tttccatctc ggtcatcatc acttcgtctg
    10261 cgacctgctc caccccacca catcatacga cctgccatac tagaaagcat ggtgatagca
    10321 ctagcaaggg caactgcaat agtcgaaata cggatatcat aattacgaat atgactgact
    10381 tcatgtccca taacagcttc tagttcttca cgattcatga tagctagtag acctgaagtc
    10441 gcagcaacag ccgcattttg aggattagaa cctgtcgcaa aggcatttaa ggctggatca
    10501 tcaatgatga aaacacgggg cataggaatc tgagcgacca gagccatatc ttccactaca
    10561 tggtagaggt ctggtgccgt ttgctcatcc acctcacgcg ctccattcat ggacatgaca
    10621 atctctgtcg attgaaaaat catagacaaa gcgtagataa agccgataat cagtgcgata
    10681 accaaaccac caagtccaga tcttataaag agataaccaa ccgcataacc aacaagagct
    10741 aagagtagga aaaataccag caacaaaatc caggtttttc gtttattgct tgcaatttga
    10801 tcaaacaaca tcttagtcac ctaaaccgct aaaatcaact ttaggaaccg acttttcctc
    10861 ttcaggtgtt tgaaggaaat ctgccgcttt aaatccaaac attccagcga taatattgct
    10921 cgggaaagtt tctaatttta cattgtagtt gctgacaaca ctgttataga gttgacgaga
    10981 gtaagaaatt ttattttctg tgtttgtcaa ctcctcttgc aatttaacaa agttagcact
    11041 agctttcaaa tctggatagc tttctgcaac tgcaaaaata cctgaaactt gacgagtgag
    11101 ggcatcactg gctttcatag cttctgctgg tgaagtcgct gccgccactt ggttacgtag
    11161 ttctgccacc ttttcaaggg tagaaccttc atatttggca taacctttta cagtctcaat
    11221 caagtttggc aagaggtcat ttcgacgttt caactgaaca tcaatctgac tccaagcctc
    11281 cttggtttgc atacgattct taaccaaacc gttatagcta acaatcacaa aaataacaat
    11341 aagagcgata actccaagaa taatccaagt catgatataa gtcctttcta cttttagatt
    11401 agtaccagta tatcaaattt ttaatgattg tggtaaaata agatgatact aaagaaggaa
    11461 ataactatga aaccagaaac attttacaac ttgcttgccg agcagaatct tccactttcg
    11521 gaccagcaaa aagaacaatt tgaacgttat tttgagctct tggtcgagtg gaatgagaag
    11581 attaatttga cggcgattac ggacaaggaa gaagtttatc tcaaacattt ttacgattcg
    11641 attgcaccca ttcttcaagg tttgattccc aatgaaacta tcaaacttct tgatatcggg
    11701 gctggggcag gatttcctag tctaccaatg aaaattctct atccggagtt agatgtgacc
    11761 attattgatt cactcaataa gcgcatcaac ttcctacaac tcttggctca agaactggat
    11821 ttgaacggag ttcatttcta ccacggacgt gccgaagatt ttgcccaaga caagaacttc
    11881 cgtgctcaat atgattttgt aacagctcgt gcggttgccc gtatgcaggt cctatctgaa
    11941 ttgactattc cctaccttaa ggttggtgga aaactattgg cactcaaggc cagcaatgcg
    12001 cctgaggagt tgttagaagc taagaatgcc ctcaatctcc tttttagtaa ggtcgaagac
    12061 aatctcagct acgccctacc aaatagagat ccgcgttaca tcacagtggt agaaaagaaa
    12121 aaagaaacac caaataaata tccacgtaag gctggtatgc caaataaacg cccgctttaa
    12181 atttttaagt aaaaaaatat ttacaaaatc aacctcgctc ttttatttct aggctcggga
    12241 aaaaatgatt tacaaaatca gcctcgctct tttatttcta ggctcgggaa aaaatgattt
    12301 acaaaatcat ttttttctgc tatactatcc taagcaaagg tttttaatgt catcccgtga
    12361 ggtgacgaag acgcaaaaat atttgaaact ctttaaagtc tagattttag agaagtctta
    12421 ctctgagggc ctattgctgt aaaataatgg gctcttttct gatgcccaaa agtgaggttt
    12481 atatgaaaca agaatcaact gttgatttgt tattagacgt tgaccaacgt ccttcggctg
    12541 gaaaaggaat tctccttagc ttccaacacg ttttcgccat gtttggtgcg accatcttgg
    12601 taccattgat tttgggaatg cctgtatctg ttgccctttt tgcttcaggt gttggaacac
    12661 tcatctacat gattgctact ggttttaaag ttccagttta tctaggttct tcatttgcct
    12721 ttatcacagc tatgtcactg gctatgaaag aaatgggggg tgatgtatct gctgcccaaa
    12781 caggggttat cttgactggt ttggtctatg tccttgttgc taccagcatc cgatttgtag
    12841 gaacaaaatg gattgataaa ctcttgccac caatcattat cggtcctatg atcatcgtta
    12901 tcggtcttgg acttgcaggt tcagctgtta ccaatgcagg tcttgtagca gacggaaatt
    12961 ggaaaaatgc tctggtagcc gttgttactt tcctaattgc tgcctttatc aatacaaaag
    13021 gaaaaggctt cctacgaatc attccattcc tctttgccat tatcggtggt taccttttcg
    13081 cactaactct tggcttggtt gactttatac cagttcttaa agccaactgg ttcgaaattc
    13141 ctggtttcta cttgccattt agcacaggtg gtgcctttaa agagtacaat ctttactttg
    13201 gtccagaagc catcgctatc ttgccaatcg ctatcgtaac aatttctgaa catatcggag
    13261 accatactgt tttgggtcaa atctgtggtc gtcaattctt aaaagaacca ggtcttcacc
    13321 gtactcttct tggtgacggt atcgcaactt ctgtttctgc cttccttggt ggaccagcca
    13381 atacaactta cggagaaaat acaggggtta tcggtatgac tcgtatcgct tctgtctcag
    13441 ttatccgtaa cgctgccttc atcgcgattg ccctcagctt ccttggtaaa ttcactgcct
    13501 tgatttcaac tattccaaac gctgtacttg gtggtatgtc aatccttctc tatggggtta
    13561 tcgccagcaa tggtttgaaa gtcttgatta aagaacgtgt tgatttcgct caaatgcgaa
    13621 acctcatcat cgcaagtgct atgttggttc ttggacttgg aggagctatc cttaaacttg
    13681 gtccagttac actttcaggt actgcccttt cagccatgac aggaatcatc ttgaacttga
    13741 tcttgccata cgaaaataaa gactaagagt ctaaatacac ctaatccact cagacagctg
    13801 agtggatttt tcgtatacca taataaaagt gtcttaacaa aattattaaa atcaaaaaac
    13861 gtataatatc agatattcta aaaccttgat actgtacgtt ttatcataga aatttttact
    13921 ttattttctc atcaaatgag atttgcatca atctcttgtc ttacttgcgt ttcttcttcg
    13981 ctttcttcat tttgttagcc atacgtttca tggactgttt catggcaaat tcaccaattt
    14041 tacctttcaa accgccacca aacatctggc tcatatctgg cattcctgct cctccgagag
    14101 ctgataagtc aggcataccg ccttgtccca tcattccttc aagggcagac atatccattc
    14161 ctcccatatt tggcatattt ttaggaaggt tatttggatt aatccccatt tgcttcatca
    14221 ttttattcat atccccagac ataacaccct gcatgagctg tttagcctgg ttaaagtcct
    14281 tgatgaattt attgacttcg acgaatgtat ttccagaacc agcagcaata cgacggcgac
    14341 ggcttggatt taacaaatct gggttttcac gttcttcagg tgtcatcgaa gacacaatcg
    14401 cacgtttacg agcaatctgg cgttcatcca ccttcatgtt ttgaagggct ggattgttgg
    14461 ccatacctgg aatcatcttg agcaagtctt ccatcggccc catattttgc acctgatcta
    14521 attgatcgat gaaatcatta aaatcaaagg tgttttcgcg catcttctca gccatttcaa
    14581 gggctttttg ttcatcgtat tcctgagaag ctttctcaat caaagtgagc atatccccca
    14641 taccaaggat acggctagac atgcggtctg ggtggaaggt ttcaatgtcc gtaatctttt
    14701 cacctgtacc agtgaacttg attggttttc cagtaatgtg acgaacagac agagcagcac
    14761 caccacgagt atcgccatca atcttggtaa ggatgacccc agtcacttcc aactgagcat
    14821 taaactcacg cgcaacattg gctgcttcct gaccaatcat agcatcaacg acaagcaaga
    14881 tttcatttgg ttgagccaat gctttcacat cacgaagctc attcatgagg agctcatcaa
    14941 tctgcaaacg acccgcagta tcaatcaaga catagtcgtt atgattagtt tgggcttgct
    15001 ccaaaccttg acgtacaatc tcaacagctg gtacttctgt tccaagtgca aagacaggca
    15061 catcaatctg ttgtcccaag gtcttaagct ggtcaatggc agctggacga taaatatccg
    15121 ccgcaatcat caaaggacga gcattttctt ctttcttgag tttgttggcc aatttaccag
    15181 caaaggttgt tttaccagcc ccttgtaaac caaccatcat gatgatggtt ggaatcttag
    15241 gtgacttgat aatttctgcc gtatcagaac ctaaaacggc tgtcagttcc tcatcaacga
    15301 ttttaataat ctgttgcgca ggattaagtg tatcaatgac ctcatgcccg actgcacgct
    15361 cacgaacttt cttgataaag tcctttacaa caggcaaggc aacgtcggcc tcgagcaagg
    15421 ccaagcgaat ttctttggtt gcctcttgga catcagattc agagattttt ccttttttac
    15481 gtagattttt aaagacgttc tgcaaacgtt ctgttaaact ttcaaatgcc atttttcttc
    15541 ctcttattct ctattatcaa tgcttgttaa aatttctatc tgctcctgca gaaagtcatc
    15601 cttgggatag cgctccaaaa tctgatcaaa aatctgactg cggacaatat agtccgagta
    15661 catgtgcaat ttcatctcat aatcttccag aatcttttct gttcgcttga tattgtcata
    15721 gacagcctga cgactgacac cgaactcctc ggcaatttca gcaaggctgt aatcatcagc
    15781 gtagtagagc tcgatataat tcatttgctt atctgtcaaa agcgccgcat aaaattcaaa
    15841 gagcgcattc atacgattgg ttttttcgat ttccataact tttattatac caaaaattag
    15901 cctaatctac cacactagga agccgatcca agaagataga tagctaaatt tgaaaaagac
    15961 atgagcctag ccccaagtaa tttccaattg atagctggca aagggatgtc cctcttgatt
    16021 ttgtagttga taatctagtt caatcttttg cctatcaact tgataatggc tcgtttggat
    16081 gataaactcc tgcatgccca taggtgtagg aatataggct aaactatcgc tatcctttag
    16141 aaagcgcata atggtcttgg gattagaaaa tcggctcatc acaagttctt gaccatgaaa
    16201 tttaatcact actttttcct tttcctcatt atagaaaagc aggtagctat aatctccttt
    16261 ttcatgcact tccacatcat aaagctggtc aatcacttcc aactgctcat caaactgaat
    16321 tgtatttcgc atccgaatct tcacatcagg tcctctttct tgtctcttgt cctactattt
    16381 taccaaaaag agcaggattt tgctataatg gtcatatgaa cgaaaaagta ttccgtgatc
    16441 cagttcacaa ctacatccat gtcaataatc aaatcatcta tgacttgatt aacacaaaag
    16501 aatttcagcg tttgcgccgg atcaaacaac tgggaacttc cagttatacc ttccacggtg
    16561 gagaacacag tcgcttctct cactgtctag gagtctatga gattgcacga cgtatcacag
    16621 aaatttttga agaaaaatat cctgaggaat ggaatcctgc cgagtctctc ttgaccatga
    16681 ccgctgctct cctacatgac cttgggcatg gcgcctactc ccatactttt gaacatctct
    16741 ttgatacaga ccatgaagcc attactcagg agattattca aaatcctgag acagagattc
    16801 accaagtctt gctacaagta gcgcccgatt ttccagaaaa agttgccagt gtcatagacc
    16861 atacctatcc taataagcag gtcgtgcagc tcatttctag tcagattgac gcagatcgca
    16921 tggactatct cttgcgcgac tcctatttta caggagcatc ctatggggaa tttgacctga
    16981 ctcgcatcct ccgagtcatt cgtcctgtcg aaaatggtat cgcctttcag cgcaatggca
    17041 tgcacgccat cgaagactac gtcctcagtc gctaccagat gtacatgcag gtttatttcc
    17101 acccagcaac acgcgccatg gaagttctcc tacagaatct tctcaagcgc gccaaggaac
    17161 tctaccctga ggacaagaat ttctttgcac gaacttcccc acatctcctg cctttcttcg
    17221 aaaaaaatgt gaccttgact gactatctgg ctctggatga tggcgtgatg aatacctact
    17281 tccagctttg gatgactagt cctgacaaaa ttcttgcaga tttatctcaa cgctttgtca
    17341 accgcaaggt ctttaaatcc attacctttt cacaagagga ccaagatcaa cttactagca
    17401 tgagaaaatt ggttgaggac atcggctttg atcccgacta ctatactgcc attcataaga
    17461 actttgacct cccttatgat atctatcgtc ccgaatctca aaatccacgg acacagattg
    17521 agattttaca aaaaaatgga gaactggccg aactctctag cctgtctcct atcgtccaat
    17581 cccttgctgg cagtcgccac ggagataatc gcttttattt tccaaaagaa atgttggacc
    17641 aaaacagcat ctttgcaagc attacccagc aatttttaca cttgattgag aacgatcatt
    17701 ttaccccaaa taaaaactag aagaggaatt ttatgagtat taaactaatc gctgtcgata
    17761 ttgacggaac cctagtcaac agtcaaaagg agattactcc tgaagtcttt tctgccatcc
    17821 aagatgccaa agaagctggt gtcaaagtcg tgattgcaac tggccgcccc atcgcaggtg
    17881 ttgccaaact tctggacgac ttgcagttga gagaccaagg tgactatgtg gtgaccttca
    17941 atggtgccct tgtccaagaa actgctactg gccatgagat tatcagcgaa tccttgacct
    18001 atgaggatta tctggatatg gaattcctca gtcgcaagct cggtgtccac atgcatgcca
    18061 ttaccaagga cggtatctat actgcaaatc gcaatatcgg aacatacact gtacacgaat
    18121 caaccctcgt cagcatgcct atcttctacc gtacccctga agaaatggct ggcaaagaaa
    18181 ttgttaaatg tatgtttatc gatgaaccag aaattctcga tgctgcgatt gaaaaaattc
    18241 cagcagaatt ttacgagcgc tactccatca acaaatctgc tcctttctac ctcgaactcc
    18301 ttacaaagaa tgtagacaag ggttcagcca ttactcactt ggctgaaaaa ctcggattga
    18361 ccaaagatga aaccatggca atcggtgacg aagaaaatga ccgtgccatg ctggaagtcg
    18421 ttggaaaccc cgttgtcatg gaaaatggaa atccagaaat caaaaaaatc gccaaataca
    18481 tcactaaaac aaatgacgaa tccggcgttg cccacgccat ccgaacatgg gtactgtaaa
    18541 aggaaagaat agactaaaac cgctcagctg agcggttttt gatgtctaat tactgccccc
    18601 tgcaggaatc gaacctgcaa ctactcctta ggagggagtt gttatatcca ttgaactaag
    18661 ggagctagat aaaaactctg ctgaaaagca gagtttttta gtcgaattaa cgacggattt
    18721 ctttgatacg agctgcttta ccttgaagag cacgcaagta gtacaatttc gcacgacgta
    18781 ctttaccgta acgaacaact tcgatttttt caacacgtgg agtgtggatt gggaagatac
    18841 gctcaacacc tacaccgtta gagattttac gaactgtgta gttttctgag attccagcac
    18901 ctttacgtgc gataacaaca ccttcaaaaa tctggatacg ttcacggtta ccttcgacaa
    18961 ctttcgcgtg tacacgaaca gtgtcaccag gacggaatga tgggatatct gtacgaagtt
    19021 gaccttcagt caagctttgg attaatggat tcattttatt ctcctatctt tgtcaatctt
    19081 gaggaatctt cctcagcgga taaactgtat ttttgtgcgt ccattacaca caagatacag
    19141 tttaccaaat ttctaaaaaa aagtaaagaa atttatagca aaattcctaa aaaaatcgca
    19201 accaaaccac ctatgtaagt caaagtcagg taagaataaa agaccttctt atcacttagc
    19261 agtctttgaa gttcgtcatt caaggtcgaa aaagttgtta aaccgccaca aaatcctgtt
    19321 gctagaatag catatacttc cttagactct acatgattgt aaaatactcc aattaaaaaa
    19381 caacctaaaa gattagctat gagcgttccg agtggcaatt tagaagcttg attatagcga
    19441 gaaaataaat agcgcactaa agctccgaaa ccacaagcaa ttgcaagata gacgattacc
    19501 atttcttcct ccctagaaaa taagctaaga gtaggcctcc accgatgctc aaaagcaaat
    19561 acaggactaa actaaaataa cgccctgtat caagcagctt cacagtatca agcattaggc
    19621 tggaaaaagt tgttaaacct ccgcaaaaac ccgtccccag tgctaaaatc aagcccttac
    19681 tagtcccctt atagaccaga tagcccttga caagaaaaat caagcagaaa attcccagat
    19741 agttgacaaa gagagtgccc caaggaaagt ctggactggc tggtaaccag gtggaaacta
    19801 gatatcggac aagtccgccc aacatagcag ctagaaaaat tcctagcgga taaaattgtt
    19861 cttttttcat ttgatatttt gatcctgata atcacgcgaa cgtttgagta tgtccgaaaa
    19921 agtcgctaca atagtctcct gatagcgctt gtcctctaca cgactcccaa ccttttcaaa
    19981 aataacttct tctctcttag tatctaaaat cggtttacct gaagctttct tataagcaac
    20041 caccccctca actaaatgca ttcgttcttc taacagtttg acaatttggt cgtcgatttg
    20101 atcgatttct tgacgaataa tatctaaatc catacagtct cctcctttat ttgaattatt
    20161 gtatcaaaaa gccatcaatt aggctagtaa aatcccaact ctcgataaga aaaatgcact
    20221 gattgattac atcagcgcac ttttatgctt atcctacttt agcaaccaat tcttctgcga
    20281 attgttccaa gcgttcaata tcttcttcct cggcagaaag atcaacttta acacactctg
    20341 aacctttttc tgctcctgtt gacacaaaga cacggtcaaa gtcatcaaca gccttacaga
    20401 attcatcgta gaaggtatct cctgagccga ccactccgta gattttgcca ttcaagttga
    20461 gatctgctag gtcttcgtag aagtccatca tctcatctgg caattcacca tcaccataag
    20521 tataggtcgc aacgatagcg atgtctgctt ccaagaagtc tgaagcgtca acagttgtac
    20581 attcatcaac atcaacatcc aagcccaagt cacgtaattt gtctgctaca atatctgcaa
    20641 tttcttcggt attaccggtc atactggcaa atacaatttt tgctaatgcc atatcgtcct
    20701 cctcaattta tctttctcca ttatatcaca tctttttcat tttaaaaata aaaattcttg
    20761 tgctacaatg aaatgagaaa gaattgaggt tatttatgga gatttgccaa caaattttag
    20821 aaaaaattaa agaatacgac accattatca ttcatcgtca tatgaaacca gaccctgatg
    20881 ccttgggaag tcaggtggga ttgaaagcct tgctagaaca tcatttccca gaaaaaacca
    20941 tcaaagccgt cggttttgat gaaccaactc ttacttggat ggctgagatg gatcttgttg
    21001 aagatagagc ctaccaaggc gcacttgtca tcgtctgtga tacagctaat actgctcgta
    21061 tcgatgataa gcgctatagt caaggtgatt ttctcattaa gattgaccac catccaaatg
    21121 atgatgtata cggtgacctg tcttgggtcg atactagttc aagtagcgct agcgagatga
    21181 ttaccctatt tgcccaaaca acccaactag ccttggcaga tcgcgatgct gagttgctct
    21241 ttgcaggaat tgtcggtgat acaggtcgct tcctctaccc ttctaccact gcacggactc
    21301 ttcgcctggc tgcttatttg agagaacata actttgactt tgcggctctc actcgcaaaa
    21361 tggacactat gagctacaaa attgctaaac tacaaggcta catctacgac catctggaag
    21421 tggatgaaaa tggtgctgct cgcgttatcc tgagtcagaa aatcttgaaa caatacaatg
    21481 taaccgatgc tgaaactgcg gccattgtag gtgcacctgg acgcattgac agagtgagtc
    21541 tctggggaat ttttgtcgaa caggctgatg gccactaccg agttcgctta cgcagtaaag
    21601 tccatcctat caatgaaatt gccaaggagc atgatggtgg aggccaccct ctagcaagtg
    21661 gtgctaattc ctatagccta gaagaaaacg aaatcatcta ccaaaagtta aaaaacttgc
    21721 ttaaaaactg ataaaatact tgccaaactt ttcagaatct gatagactag tatggtaaca
    21781 atctatggct cgcaaagaga ccatggcaga aaggaaatat tgcaaaatga aaaaagatat
    21841 ccatccagaa tatcgcccag ttgtcttcat ggacacaact actggttacc aattccttag
    21901 cggttcaaca aaacgctcta acgaaacagt tgagttcgaa ggcgaaactt acccattgat
    21961 ccgtgtggaa atttcatcag actcacaccc attctacacc ggacgtcaaa agttcactca
    22021 agcagatgga cgcgtggatc gtttcaacaa aaaatacggt ctcaaataat gataagagaa
    22081 cagttttggc tgttcttttt tgtttcttga aatcaactgc tgttttcatg ttccagactc
    22141 atctgtaggt tcggtttcca tgctactagg caggaaggaa atagctgttt caacacgtcc
    22201 ataatgagct gtactattgt cacgaaccac actttcattg atggtccaag tggaattcat
    22261 tttcttaaaa gcttctcgga ctttttccaa atctttggag gcaatggcct gttctaaggt
    22321 ttcaaaacga ggacttagac ggtttaccaa tttttccttt tccgcatcta ggtcaacagg
    22381 attttgttct ttttcaaaag ctaatagagc tgaggaaatt tcggtgagtt tggcttcagt
    22441 aatatctcca gatagagcta acttttcctt aacaatctta ccaacctcag aatccttatt
    22501 ttctactctt tcgaagtctg atgccatctt cgtaacgagt ttctgggtct cagtctgacc
    22561 accattttta accgttttgg atgcatctgt gattttcaca aagaaaaaac tcaagctttc
    22621 ttgggcacct gctggggaaa gaattaaaaa tgacaaagct aaaactagta accagctttt
    22681 agccttccaa taatttctga ccaatgtagc ctcctttctc cacaccacca aagcaagtaa
    22741 aaagtccact acctatgtga gtgatgtatt cattcatctt atcagtagca cctaggttag
    22801 tttgaacctt aacaaagtta tcaggatcct tctggaaaga aatgaagagc aatcccgtat
    22861 caaactgacc agtcttttca tcaatgccat ctgagtaaga ataagaacga cgtaaaagtg
    22921 gcttatccac ttctttggcc aaacaaacat gcgaatcatc aggtaagaga ctcaaatcca
    22981 cttcatcaaa ctcatttttc ttaccaaagg gggcaccgct ttccttgtaa cgaccaaagg
    23041 tattttcttg ttcttcgaga ctagtgcggt cccaggtctc taaaaacatc tgaatccgac
    23101 gaactgccat ataagaacca ttttccatcc agtccttact atcagcccag ataacgcggt
    23161 caaagtcttg ctccttggtt ggatttgctg ttccatcctt aaaaccaaag aggttccacg
    23221 gagtctccat tcgatccccg atagctgcaa atccagactg actccaacga aggggcaccg
    23281 catttctccc cttacggatg agattgcgaa tagcgtgaaa ggcaatttgc tcatcatctg
    23341 cacaagcatg aatcacaata tctccaccag tatatttttc acgtaattgc tccttaggaa
    23401 atagaggtaa atccctgaac agtcgagggc gcttgttttc caagttcatc cttttcaaga
    23461 aacttgcaga tacaccaaaa gtcagcgtca aacgatgagg attgagccca actgtctcac
    23521 cagtatcgct cggaggaaag agagcattct gtccatcttt tttaaccaat tccccctcga
    23581 ccaacttggc actataatcc gtccaatcct tgaatagctg gataatttta tctctatccg
    23641 tcgtatgcag gtccaagaca acaaaataga tattcttttg catgggcgta ctatttggta
    23701 catcaagcga accttatgaa aaggtctact ggttttaaag cgctttcttg tcttcttgaa
    23761 ttgagatttt atttttagac gcgacaatgg tttgagcgtc tgctttccta ggatgttctg
    23821 gacgtgg
//
LOCUS       DAVKLX010000033        22427 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKLX010000033 DAVKLX010000000
VERSION     DAVKLX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22427
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.033"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(84..545)
                     /locus_tag="VW325_001408"
     CDS             complement(84..545)
                     /locus_tag="VW325_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9734785.1"
                     /translation="MGKERREQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(512..655)
                     /locus_tag="VW325_001409"
     CDS             complement(512..655)
                     /locus_tag="VW325_001409"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEU9734786.1"
                     /translation="MAYSTDFKQGALDSIKGGHRHVEAAKVFDVGVRTLFTWEKKDVS
                     KGT"
     gene            924..1304
                     /locus_tag="VW325_001410"
     CDS             924..1304
                     /locus_tag="VW325_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9734787.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1403..1624
                     /locus_tag="VW325_001411"
     CDS             1403..1624
                     /locus_tag="VW325_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9734788.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1641..1955
                     /gene="trxA"
                     /locus_tag="VW325_001412"
     CDS             1641..1955
                     /gene="trxA"
                     /locus_tag="VW325_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9734789.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            2330..2794
                     /gene="queF"
                     /locus_tag="VW325_001413"
     CDS             2330..2794
                     /gene="queF"
                     /locus_tag="VW325_001413"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9734790.1"
                     /translation="MQHYETVEAVTFAYGQRHHLEIQITREIAKEQGIRHHILDMSLL
                     GQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNLLD
                     PRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR"
     gene            2856..3248
                     /locus_tag="VW325_001414"
     CDS             2856..3248
                     /locus_tag="VW325_001414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734791.1"
                     /translation="MFEVVDKTDEVSSKHCFEVADRTDEVSLKHCFEVADRTDEVSLK
                     HCFEVVDRTDEVSLKHCFEVADRTDEVSLKHCFEVADRTDEVSLKHCFEVADRTDEVS
                     NHTYDKVKLTWFEEIFEEYHTKKPCSSR"
     gene            complement(3273..3941)
                     /locus_tag="VW325_001415"
     CDS             complement(3273..3941)
                     /locus_tag="VW325_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9734792.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGPIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(4052..4843)
                     /locus_tag="VW325_001416"
     CDS             complement(4052..4843)
                     /locus_tag="VW325_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU9734793.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     AIGIQEELFTQEQFHFIAEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLYADANQ
                     QSVFYQGLQLEIRNVFSNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFSKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            complement(5090..6886)
                     /gene="pepF"
                     /locus_tag="VW325_001417"
     CDS             complement(5090..6886)
                     /gene="pepF"
                     /locus_tag="VW325_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9734794.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(6897..7640)
                     /locus_tag="VW325_001418"
     CDS             complement(6897..7640)
                     /locus_tag="VW325_001418"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9734795.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGSKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(7642..8592)
                     /locus_tag="VW325_001419"
     CDS             complement(7642..8592)
                     /locus_tag="VW325_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9734796.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNMD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            complement(8730..9158)
                     /locus_tag="VW325_001420"
     CDS             complement(8730..9158)
                     /locus_tag="VW325_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9734797.1"
                     /translation="MEIEISDFTGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKIYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(9160..10227)
                     /locus_tag="VW325_001421"
     CDS             complement(9160..10227)
                     /locus_tag="VW325_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9734798.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKELAEGV"
     gene            complement(10246..10716)
                     /locus_tag="VW325_001422"
     CDS             complement(10246..10716)
                     /locus_tag="VW325_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9734799.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(11007..11459)
                     /locus_tag="VW325_001423"
     CDS             complement(11007..11459)
                     /locus_tag="VW325_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9734800.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(11496..11669)
                     /locus_tag="VW325_001424"
     CDS             complement(11496..11669)
                     /locus_tag="VW325_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9734801.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKIEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            complement(11812..11991)
                     /locus_tag="VW325_001425"
     CDS             complement(11812..11991)
                     /locus_tag="VW325_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734802.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            complement(12156..12394)
                     /locus_tag="VW325_001426"
                     /pseudo
     CDS             complement(12156..12394)
                     /locus_tag="VW325_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12664..13935
                     /locus_tag="VW325_001427"
     CDS             12664..13935
                     /locus_tag="VW325_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9734803.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKASGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            14199..14271
                     /locus_tag="VW325_001428"
     tRNA            14199..14271
                     /locus_tag="VW325_001428"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14232..14234,aa:Lys,seq:ctt)
     gene            complement(14481..15800)
                     /locus_tag="VW325_001429"
     CDS             complement(14481..15800)
                     /locus_tag="VW325_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9734804.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKNGVRQVQNMAFSDDGIHFEKIEQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVEWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(15810..17426)
                     /locus_tag="VW325_001430"
     CDS             complement(15810..17426)
                     /locus_tag="VW325_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9734805.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            complement(17455..18345)
                     /locus_tag="VW325_001431"
     CDS             complement(17455..18345)
                     /locus_tag="VW325_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9734806.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            complement(18356..19273)
                     /locus_tag="VW325_001432"
     CDS             complement(18356..19273)
                     /locus_tag="VW325_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9734807.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            complement(19425..20426)
                     /locus_tag="VW325_001433"
     CDS             complement(19425..20426)
                     /locus_tag="VW325_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9734808.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            complement(20788..22269)
                     /locus_tag="VW325_001434"
     CDS             complement(20788..22269)
                     /locus_tag="VW325_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9734809.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
BASE COUNT         6834 a         4839 c         3914 g         6840 t
ORIGIN      
        1 taagtttttt ataaagacac gcaaatgagt ataaatcagc taatttttgg tttacattcc
       61 tatttttatg cataattatt ctgtcattct ctaatttgtg aatcaatata tctgattaga
      121 aaactaccat tgagtttttg ctccacctca ttagtccgct caaaacgccg tccgctaatc
      181 ttgtcataga ctttctcccc tctaggagcc cctgcataag gacgatagag gtagcggttg
      241 attcccgttt cgtcaatata taggactggt aaatctttta ggttatccaa aatatcaaga
      301 aactcagaaa ctttctcagg cttttgttcc ctaaaactgg tcgtcttttt taaaatgacc
      361 ttaatctgct ttaaaactgc ccatacggag ggcaaagcac aatcaaaacg ggccgcaatt
      421 tcccgtaaaa aagcgtctgg atgagcctct acaaaggctt tcaattcttc taaagggatc
      481 ttacgctttt tgacgactcg ctttttccgc tctaagttcc cttgctcacg tctttctttt
      541 cccacgtgaa gagagttctg acgccgacat caaaaacttt ggccgcctcg acatgtctgt
      601 gccctccttt gatggaatct aatgctcctt gtttaaaatc agtactatat gccatagctt
      661 tattagacca tgcttatttt aaactaagca actatagata gagaaatgaa ataaaatctg
      721 aattcgaatg tgataaggag ttagaaattg aaacaatagg attcccccat tattcacaca
      781 aaaataaaac cactaaaaaa cagagcttgc cagcattctt gaaaagtagt ataattattg
      841 catgcgcaat catcttgtga cgcagagact gcccgttaat ggactttctt ctattttatg
      901 actctaaaaa gaaaggagac actatgacct atttggaaaa atggtttgac ttcaatcggc
      961 gtcagaaaga aattgaaagt ctcttggaag agaccattgc ccaacagagc gagcaaagtc
     1021 tgaccttgaa agagttctac ctgctctact atctggactt ggctgaagaa aaatctctac
     1081 gccagattga cctgccagat aagcttcatc tgagcccgag cgcagtttct aggatggtgg
     1141 ctcgcttgga agctaaaaat tgcggtctac tcagtcgcat gtgttgccat caagatagac
     1201 gctctagttt tatctgcctg acaaatgatg ggcaaaagac actggcctcg ctacaaaaga
     1261 ctgtcgaaga aagcttggaa gcaggtttgg atttcttgat ttaagatgat ttttaaaaaa
     1321 gttgcgtgcg caatcatttt tcttgacatt ctcttttata aggagtaaaa taaagttagc
     1381 attaaacaaa ggagttttaa atatgattga aattacctat atagatgcca gcaagaacga
     1441 aagaactgtg actttcgagt cttatgaaga ctttgaacgt tcgcaacaag cttgccttat
     1501 cggcgtcgca gactactacc ctgtccaaaa attaacctac aagggtcata atttggacta
     1561 ccatgggact tatggagata tcttcttcta tctcatgaaa caagatttaa gccaatataa
     1621 ctaaaaaaag gagaaataca atggcaaaag caattacaga tgcaacattc gaacaagaaa
     1681 caaaagacgg tttggtctta gtagacttct gggcaacttg gtgtggtcca tgtcgtatgc
     1741 aaggtccaat cttggacaaa ttgtctgaag aactttcaga agatgtcttg aaaatcgtta
     1801 aaatggacgt tgatgaaaat ccaaacacag ctcgtgcttt tggaatcatg tctattccaa
     1861 ctcttctctt caaaaaagac ggccaagttg tcaaacaagt tgcaggtgtt cacacagcag
     1921 aacaaatcaa ggccatcatt gctgaattga gctaatcaaa cgagagacca agtactttct
     1981 ttggtctctt ttttcttgcc ctttgccatt tttcaaaaaa tatgctagac tatagtagat
     2041 tgaaactaga atagtacatc tctacttcta aaacattgtt agaaatcgat ttgactgtcc
     2101 tgatcgattt gtcctgttct tatttcactt tactatataa atagaatatt acgatatttg
     2161 ggatatgcca tcgctaaaaa aaacctcgat ttggtatttt ttagctccct aatgggagct
     2221 ttttacgtgc tctgaacatt tcccattttg gaaggagtac tatgaaacgt caatcagcct
     2281 tggtcgtctt agtggtggtc aagattcaaa aacctgcctt ttctggacca tgcaacacta
     2341 tgaaacagtc gaagctgtca cctttgccta cggccaacgt catcacctcg aaattcaaat
     2401 tactagagaa atcgctaagg aacagggcat tcgtcaccat atcctcgata tgtctctgct
     2461 gggacaaatc actgctcagc cagactttgc gacgattcat atttcctaca ttcctgacaa
     2521 gctctgtgtc gagtcaaaat ccctcaaact atatctattt agctaccgaa accacggaga
     2581 tttccacgaa aactgtatca acaccatcgg gaaagacttg gtcaacttgc tagaccctcg
     2641 ctatttagaa gtctggggaa aattcactcc gcgcggtggc atttcaatcg acccctacta
     2701 taactacggt aagcaaggaa ctaagtatga gggcttggca gaacaacgcc tcttccaaca
     2761 cgacctttat ccagagaaaa ttgacaaccg ctaaagtcat actcaatgaa aatcaaagag
     2821 caaactagga agttagccgc aggttactca aagcagtgtt tgaggttgta gataaaactg
     2881 acgaagtcag ctcaaaacac tgttttgagg ttgcagatag aactgacgaa gtcagcttaa
     2941 aacactgttt tgaggttgca gatagaactg acgaagtcag cttaaaacac tgttttgagg
     3001 ttgtggatag aactgacgaa gtcagcttaa aacactgttt tgaggttgca gatagaactg
     3061 acgaagtcag cttaaaacac tgttttgagg ttgcagatag aactgacgaa gtcagcttaa
     3121 aacactgttt tgaggttgca gatagaactg acgaagtcag taaccatacc tacgacaagg
     3181 tgaagctgac gtggtttgaa gagattttcg aagagtatca tacgaaaaag ccctgttcct
     3241 caagatgagg agcaaggctt tttgagtttc aattattctt ctgttccaag gaaatttttt
     3301 gcaacaaggg ctgcaagaac tccaccagcg attggtgcaa ggatgaaaat ccaaacttgt
     3361 tgaagggctg cgccgcctac caagacagct ggtgccaagc tacgagctgg gtttactgaa
     3421 agtccagtaa tcttcaatcc gacaagaatc atcgccatca atgacaaacc gattaccaaa
     3481 ccagcaatcg ggccattgcc cttgctttct gaagtcacag tcatgataac caatacaaac
     3541 aagaaagttg cgatgacttc aaacaagaaa ccaccaaaga cagtgacacc gtttgccaag
     3601 gcattttcac caagactagc agttgacata cctgagttag ccaagaggaa gaagacagcg
     3661 ccagaagcga tgaaagctcc aacaacctga ccaaggatgt agtttacaag ttctgaagat
     3721 gacaaacgtt tgtttacaaa catagcaatc gaaacagccg ggttcaagtg agcacctgaa
     3781 acagttccga ttgagtaggc tgccaccacg attgccaaac caaaggcaaa ggcgattcca
     3841 aggtgaccaa ggccatcaaa accatttcca aaaacaacag ctcctgtccc aacgaacaca
     3901 agcatgaacg taccgattaa ctcagcaaca aattttttca ttttctttct ccttttttca
     3961 aaaactagat actagtctat caaaagtagg aaagggtttc aagaaaattg attggaaatt
     4021 ttttgaaaat catagaacta ttagctaatc tctagtattg aaaagactgg atagcttctt
     4081 tcaactcatc ttgtaaacta tttctctggt caagttggac atagacttcc accagacagg
     4141 atctaaagtt ggaaaatttg gaaaaatcct ccctttcttc tatcggaaaa tcaacagttt
     4201 ttatccaaga agctacttgt tcttgctcca acttcccttg taaaataggt tcatagatca
     4261 ctcttgctaa acgccaatcc tcatcatctg taaagcgaat cgacattctt ttaaatagtt
     4321 ggccaagtat gtcaaatact tcatgaattc tgtttatagg aaagtctgga tgacagacca
     4381 cctctgtcag taaatcggct ccatgtgcaa aagcatgaac ccaaccatac tgacttgaga
     4441 aacctgttgt atccttttct tttgaaagat agtgcaagcc ttgatttgaa aagacattac
     4501 gaatttctaa ttgtaatccc tgataaaaaa ccgattgctg gttggcatct gcatacaaga
     4561 gatttgcata aacaagtgcc ctaaaagaac gttcaagtgt tggcagacct accttatcaa
     4621 tctctttatc tagtcctaca tcagatgaaa ccccttcagc aatgaaatga aattgctcct
     4681 gtgtaaatag ctcttcctga atccctatag caaagcttgt aaaaacaagg tcatcgcgaa
     4741 tttctggaga aggatctccc aaatgatcaa gcaaccactg gatttcctcc tggttataat
     4801 ttggtttttc ttctgctatt tttcttagta aatcttgata catggtcaat acctctacat
     4861 ttctagcaac tgttcaaaaa ggcagtctta aatgactcaa tattgaattc tcaattaaat
     4921 acaatctgat ataaaatgac gtaaataact atcaatacca gttctacagt aagttcaaat
     4981 ttaacatcac gaccttcaac gacatttttg aaaatagcta caactaagac aaatagaatg
     5041 acgcttaaca agcccataaa catcattcta aaaaattttt ctattcccct tactccccca
     5101 actcagcact gtaggcgata atctggtcaa ctgtgtcaga caagaattgg atggtatcac
     5161 ggagtggttt gtctgttgaa atatcagcac cgataatcat ggctgactca agtggtgtct
     5221 tgctaccacc tgatttgagg agattgagcc agtcttcagc tccagtttct gagtttttaa
     5281 gatgaaggta accagctgtc gagatgacaa gaccagcaga gtaagtgtaa ctatacaagc
     5341 ccatatagta gtgagcttgg cgcatccaag tcaaagctgc atcgtcgtcg atttcaatgg
     5401 catcgcccca aaaatctgtc aaaacttcct tcataatact gttgagtttg cttgctccga
     5461 aggtttctcc ttcttcaatc aatgtataca ccttacgctg gaaggcggct tccaagaggt
     5521 gggtgataaa gttatggaag taggtgtctg tcaagcgatg agccagagca aagcgttttt
     5581 gacgtgggtc attagattgg ttctctaagt aatcactgag tagcaattca ttgaaggttg
     5641 acggtgcttc aacatagtag gtcgacatat gggcattgaa gtaactttga tgattgtctg
     5701 aaaagatgaa ttgaccagaa tgcccgattt catgaatcaa ggtatagaca tcgctcaaac
     5761 ggcctgtcca gctcatgagg acataagggt gtacgcgata tgggtccgcc gcataaccac
     5821 cggaatcctt gccactgtta gcagcaaagt ccacccagcg ctcttcttgg taacgagcaa
     5881 cttcctgaca atattcttgc cccaaaggtt ctaccgactt catgaccaaa tcataggcat
     5941 cgtcaatagt cacttcagga ttcagggcgc tgtccaagtc caatttccag tctgcaaagg
     6001 tcatcttttc aagaccattt accttggcaa catgcttgag gtatctctga gcgactggtg
     6061 caaagtcctt catgatgagg tcaatctggc ggtcaaacat gacacggtcc acttcttgtt
     6121 cagctagaag atagtcaaag acagagtcgt atcccttcat atcagccaag agtttttcag
     6181 acttgacctg agccagatag gctgctgcag ccgtattttg gtgcttacga agtccctctg
     6241 agaaggaacg gaaggatttc tcacgaacct cagcatcctc gtgattttgg tagaaattct
     6301 cataggtgac aaagctgttt ttataggtct tgccatgggc ttcaaagtca gccatttcaa
     6361 aatccccagc tcgcatctta gtataaatgt cctgcggact atagaaaact tcaccgagat
     6421 tggtcaaggc cttctccaca tcagccccta gatagtgggc ttttttgatt ttggcctgac
     6481 gaatagctgc cgtcaagtga ggcaattcac ccaaacggtc caagacttcc tcgtctgcta
     6541 ccaccaaggc atcgtcaaag aaggtcaagg ctacgctggc atctgtttca aattccatcc
     6601 cagcttgggc aatattggca aattcgtcat tgctatagtc cgtcgtctga ggcataaaag
     6661 catagttgcc aatatggctc atctgaatgt agatctgttc caattccgca aaggccttct
     6721 cgaaatcctc aaaagtatga agattaccct tgtagtcacg gctaaattgg ttgatgtctt
     6781 cacgagcttt ctcgattgca cgcaagaaat cctcacggtc ttggtatagg gctgttaagt
     6841 cccagagttc cttctctgga aattctgaac ggtgtttttg ttccattttc ttcctcttat
     6901 ttctctaatt ctactaaaac actaagggct gataaggcgt aaagcggtgc tgtttctgct
     6961 cgcaaaatac gaggacctag gcctgccaaa acggctcctt tagcttcaaa actttcgatt
     7021 tctgcaggtg acagaccacc ttctggacca aagataaaga gcagtttgga tcctgtttca
     7081 agaccagtga ctgcttgcag aagcgcagcg gcttctcctt ctttagctga ttcttcatag
     7141 gctactatga tagagtcaaa ctggtccagc tgagctagaa aatctgcttt tttctcgaaa
     7201 agtttaatac ttggtacaat attacgcttg ctttgctcgg ctgctccaag ggcaattttt
     7261 tctagttttt caactttttt acccaatttc ttgccatccc acttggcaac tgaccagtct
     7321 gcaggaaagg cccagatttg gctagcaccc agttcggtta ctttttgagc gatgaactcc
     7381 agcttgtctc ccttgggaaa tccagatgcg atggtcactt ggactggtag ttccacattg
     7441 tcatttaatt cttggaccaa ctcaaactga cgattttcca tatccagcac gcgcgccaag
     7501 cgcttgatgc catcatcaaa gactaaggta acctcatcct cttctttcaa gcgcataacc
     7561 tgaaacatat gcttactggt ttccttgtcc tcgatagtga caggagagat agcactgcct
     7621 tttacaaaat actgctgcat gctagcctcc aatcacacca gagatatcct tggttttctt
     7681 aaagacacag gtattccatt ccccttgaac catgtgagtt tcgaggaaaa atccagctga
     7741 ttcagccgac tcgcgcacca tgtcccactt atccttgata atgccactca tgatcaggta
     7801 gccttcgtcc ttaaccaaac gataggcatc gtctattaga tgaatgagga tatccgccaa
     7861 gatattagcc acaatcacat ctgcctcaat ttccacaccc ttaagcaaat ctccagccgc
     7921 tacatggata ttttccatgc cagggttgag ctcaatattt tcctgagcca cacgaaccgc
     7981 cacatcatcc aggtcatagg cgaaaatttc tttagccccc agaagcgagc tggcaataga
     8041 gagaacccct gaaccagtcc ccacatctag caccgtttcg ccaccacgaa gaacctgttc
     8101 caaggcaaaa aggctcatct tggtagttgg gtgggttcca gtaccaaaag ccatgccagg
     8161 atccagcttg ataatcattt cccccgcagt cgcctcatag tctgtccaag agggaacgat
     8221 ggtcaaatca tgagtgatac gagctggctc atagtatttc ttccagttgt ctgcccagtc
     8281 ttcctcagcc aaggcagtcg tacctatttt taactctccc aaatccataa aatccgtcaa
     8341 ttctgctaag cgagcctgca agtccgcctc aaccactgtc acatccaccg tgtcagggta
     8401 gtaggctgtc actacgattt cttcttgctg ctccacctct gggaaaatct cgccaaagcg
     8461 gtccatattt cccacatagt ccatgctgtc ttcaatcgca acaccttgcg ctcctagctc
     8521 aatcaagaga ttggaaacca actcctctcc ctcacgcttc actgtaactt ttaactcttg
     8581 ccatgtttcc attattaaga taccaagccc gtaaaacaca aaaccaaaat aggaaattct
     8641 ctgaagacgt ttgtgtctaa gagaagttta tctttttggc accgtgttta gggcgggttc
     8701 agtttagaaa tttaaccgaa ccatcctttc taatcactta cttttaaata atcttttaat
     8761 ctctcttgca actgaggcac aacttgactg gaactaagaa attcctcaac attcatcagc
     8821 tgatagccct gtccttcatc tccaaatacg atactgtcaa actggttctg tgtcaactga
     8881 ccaactagaa aaacggactg tttgtctgca aaaagcatac ttgggtaaat cttactccaa
     8941 agcagacaat cttcagtcag atgaatgccc agttcttcat aaacttcacg cgctacacac
     9001 tcaaaaggac tttcatcacc ttcacgtcca ccacctggca gttcccacat attggcccag
     9061 ggaatgcttg ccttatcatc gcgtaagata gtcaaaagct tatccccaca aaacaaagca
     9121 atcttgcaac ctgtgaaatc agaaatctct atttccatat tagactcctt cggctaattc
     9181 cttttccagc tctgctaaca agttttcata atagcgtttc tcgtccctca acattcgact
     9241 actatccatt ttctgtctag caatcttgag agccttacga gttcgatcta catctttctt
     9301 cacctttaat tgataccagg cttgtatcac ttgaagattg gacagtttga gagacagaaa
     9361 cgatttgacc tgtcgaatac tggcatattg ctccgcttgc tcaaaatctc cttccaacaa
     9421 ggcgatatga agcagggata gttgggcaac tgtctgcatc atcggagtag ttgtcctctc
     9481 aagtaatgct tgaaactgct gtttagctac ttcttccttc ccttccaaaa tggaaacttc
     9541 accttgcata cctaatacac catccgcaaa actccctcgt gcatcctcag gaactgcttg
     9601 aacaaagtct ttcaaatcat attcttgagg agccagcaaa gtttgagcag aatgtctcaa
     9661 taccaggtag gcgtatttgg tattttcagg gtgttgtagt aattcccaaa tttttgctcc
     9721 atcggtgatg tcgactggca aaatgttatt taggaagaaa gataaattaa gaaaaatcca
     9781 agtccctgca aaataccagc ttcttgtcaa aaatccaaac actatcgcca ataatatcaa
     9841 gccgagatga accatcaagc ctcctgaaag catcaggatg attctttgat cgctttcatc
     9901 ctcttttaaa ccaatgtatt gagcaccaac atttttcaga atggctgttc tactaagatg
     9961 aaacctgcct gactttttgg tcaaaataaa atgtcctaat ccaaaagcca ccagccgata
    10021 gcctgtcaag tagccacaaa aagcatgacc cagctcatga agaataaaga ttaaatacat
    10081 gcttagaaga gcgaaggcat aaccaaaagt aaaggctaaa actgcggaat accccaactc
    10141 tgcaaatgcg attgttccac aagcaaaagc tagcataata aagacaacag ctagcacata
    10201 aaccaaataa gtcccaattt tcttcataac acctccaacc aactcctagt atcttggata
    10261 aggataaaat tctccctctt ccaagccaac ttttccttct tcaaagactt cttgattcca
    10321 ttccataaca aattcctctg cttctggatc ttccaaaaag tccatgaggg tatctagccc
    10381 aacctccgca gtatctttga ggaaaagggc aaaataagct aaaaactcgc gagaaaatcc
    10441 ttttttaggt agataaggga taaccgttaa atagtcttct gcattgacgg ttgacttggc
    10501 aggattgtag aaaaggaccg cttcctcaaa aagaatgtca tctgatgaaa cctctccgtc
    10561 ttcatccacc atctccacac ctgcagcatt ttgcgcttcc aatagaaaac tcacttctac
    10621 cgcgtggtta cgcttgtccc aactaatctc aaagtcaaag ggaaagttct tgtccaactc
    10681 ttcctctaaa atatctaaaa atccgtatgt tgccattttg tcctctttct atgcgactct
    10741 ttaatcgccc cgattgctcg gaaatatgct aaaatagata ctaccatctt accacaaaat
    10801 tattttatgt cctaattata ccatattacc tcatttaaac ccttggtatc agtgattttc
    10861 ttaaaagtct gatttcttca tttctcataa aaatcaatat aaaaagccct cgaaagggct
    10921 aataaatcta taaaatcaat aggcgagtaa ctagcacaag tggacgtgct tttttattga
    10981 ctattaccac gataccacgc ttaatcttag gcttgaactt tcttatctgc aatagcgtct
    11041 gtcaaagtct gagaaaagtt aagtcccatt tctcgtccca acttatctgc ccattttggt
    11101 atggtcaaag tctttttaat gggttcctga cttcctaggt attctgatac atcaacagat
    11161 accatagaaa taaaagattt atcaaggtca taggttgaca cgaaatcttc atcatcttta
    11221 aaaggatcat tatcaattaa agacaagcta ttgatatctg atggctgagg taactctcca
    11281 tcactctcta tcaaatctgc aacagttatc cctagccact ccgaccccat agccaaagcc
    11341 tcagaaatcc cctctccttg tgtagctgag tattcaaaat ctgggaaatg gacaaaataa
    11401 gtcgcttctg ttccgtctgt gtcgtcataa taaaataaag ctggatacgt aactaacatt
    11461 tcactacctc catatcaaaa agcagggact gaattttaca acccagcttg ctttcctatc
    11521 cctctttcag tgtacttatt cagctcacca tgaaggattg tgataggtct ttccccttgc
    11581 ttctcgattt taatatggga gcctttaccg cctctagtct ttatccaacc atgggccgta
    11641 aggagtttaa ccatctcttt ttgtgtcata ggtatagcgc ttttacctcc tgacaacacc
    11701 attataacac gtgttacacg tattgtaaag gagtgatact tattattcta ttatacataa
    11761 aagcccctag atgtggttct aagggaaacc aatttattca tacctatttt tctaatgagt
    11821 agtaaaaact gcttctttat cgagcaattc atcatctgta tagtcaattg taaaagtatc
    11881 tcgatctaag acagattgag gcggagttga atgaatcata ggaccactgc gtactctata
    11941 ttttttatct ccaattttta caaactgata ctcttcgaaa atcaaattca aaccacgtca
    12001 acgtcgcctt accgtactca agtacagcct gcggctagtt tcctagtttg ctctttgatt
    12061 ttcattgagt atgattaact ctcaagtctt cgaaatcagg attttcaaca gttattacaa
    12121 ggaggcgatt tactacttca aaaacatcaa ttattctatt tttcatattt tttcaaccca
    12181 ttattagaat gaacttcttg gtaagcaaaa tcaagtttag atttaatgtt ttcgtacaaa
    12241 tctaaaatct cttttggagt atcttccgga agaaaagttt tcttttccct gaaataactt
    12301 gatcactaag aatccaatga cgaatttgtt ttgtaaaaat caaaatttcc tgacttggtc
    12361 catcatttcc attgcttatc acctctcttt tcattatagt tcatacaatg acattcagca
    12421 atattatttc tcaagtcagc acttccactt ctttaggctc aactatccta ttttgagctt
    12481 taaggaaaat caaatctctc atgctgatac ctctcctcat taaattaaat agtaaaaaag
    12541 attctatctc actccctgat tattacaaaa ccattgaaat atcacaacta ataggctaga
    12601 atggacatag taagatatag tagatgagtc attctactca aatccacgct agaaaggact
    12661 gctatgccag acaatctcgc gcttcgcatg cgccctaaaa ccatcgacca ggtcatcggt
    12721 caggagcatc tggtcggacc tggaaaaatc atccgccgca tggtggaagc caaccgcctg
    12781 tcctccatga ttctatatgg ccctccggga atcggcaaaa ccagtattgc ctctgccatc
    12841 gctggaacga ccaagtatgc ctttcgaact ttcaatgcga cagttgatag taaaaagaga
    12901 ctgcaagaaa tctcggaaga agctaaattt tctggtggtc tcgtcctatt gctagacgaa
    12961 attcatcgac tagataagac caagcaagac ttcctcttgc ctctcttgga aagtggactg
    13021 gtcatcatga ttggagcaac gactgaaaat cctttctttt ccgtcactcc tgccattcgt
    13081 agccgtgttc aaatttttga gttggaacct ctgtctaacc aagacgtcaa agaggccctg
    13141 cagatagctc taagtaaccc tgaacgtggt tttgattttc caatagaact agatgaggat
    13201 gcgctggatt tcatcgcaac ctctacaaac ggagatcttc gctctgcctt taactcactg
    13261 gacttggctg ttctctctac ccctgagaat gacgagggca ttcgccacat caccttagac
    13321 atcatggaaa atagtcttca gagaagctac atcactatgg acaaggatgg agacggtcac
    13381 tatgatgttc tatctgccct gcaaaagtct atccgtggct cagatgtgga tgccagtctc
    13441 cactatgctg cccgcttgat tgaggctggg gatctgccta gtctcgctcg tcgcttgact
    13501 gttatcgcct atgaagatat cggtttagcc aatcctgagg cccagattca taccgtgact
    13561 gctctggatg ctgcacaaag gattggtttc ccagaagccc gcattctcat cgccaatgtc
    13621 gtgattgatt tggccctttc tccaaaatcc aactcagcct atgtagctat ggataaggca
    13681 cttgctgacc tcaaagcatc agggcacttg cctattccgc gacacctgcg tgatgggcac
    13741 tacagtggaa gcaaggaact ggggaatgcc caagactatc tctatccaca caactatcct
    13801 ggaaattggg tcaagcaaga ctatctgcca gaaaaaattc gtaatcatca ctatttccaa
    13861 gcagaagata ctggtaaata tgaacgggct ttggctcaaa gaaaggaagc tatcgaccat
    13921 ttgcgaaaaa tctgaaatcc ttttcaaaaa actgcacttt cctcttgatt ttttttgaaa
    13981 aagtggtatc atataaatat agaaacgctg tggtgtacga cttcacactt aagtgttgac
    14041 cgactatttt ttgtattatt agggaaacaa aagtcttcta acagcatgta ggccgtctca
    14101 cacggaaaca gcttcagtta gagcgagttg cccacctgct taattgcgcg ggttcaatac
    14161 aaaccgtgaa gtttcggcac caatacagct ttttatttgc cttcttagct cagttggtag
    14221 agcagtagac tcttaatcta tgggtcgcag gttcgagccc tgcagggggc atctaaatca
    14281 acaggaaaaa gccttataat atagggcttt ttttgcttaa ataaaatttt tttcaaattc
    14341 tttagaaaac tagtttaaat cataatccaa caccaatttt acaaatacca taaactaagc
    14401 ttatataccc aaaacacaaa aagtcctctt tccactattt tagaaagaga actttttctt
    14461 taggagaaat aacttaattt ttaatcaatt cgtgatagct cagctggcac cgttaagtaa
    14521 taagttgcag tcaagcttgc ttcaccttga ttgacaaaaa tctcgatgga atttttatct
    14581 agaacaactt ccaattcttt agcttcaata tctacatacc gtcgactagt gtcctgttct
    14641 tcttcaccta gaattttttg aataagatgg ctacgatcaa tgtaaacttg ctgcgcatta
    14701 ctgtcataac caaattcaag ataatctgta tcatttccta agtggtaatg acaatcttta
    14761 tctatttgga tttgatattg gcctttttta acagggaatt gtcttagttt gccatcttcc
    14821 aatcttagaa ttctaggtaa agtcatggca catgcccact tgtgttcttg gtcatgggtt
    14881 ggaagggtac gcccccatgt ctgcatccaa gcaatcagga tacgacgatt ttgatcgtcc
    14941 aacaatgttt gaggcgcata gaagtcttgg ccatgatcaa tttcttgaac tgattctggg
    15001 ataaaacatt tttctcccca ctctacctta cccgtgaaca aaagcgatga gttgatgtta
    15061 tgatatgagt ctccctcacg ctgataacgc atgggtgaca taataaggca atctttccca
    15121 tctaactcga agtaatctgg gcattcccac ataaaccctt ggtgttctac cccttttaaa
    15181 aagatggatt cgaactgcca ttctactagg ttatcggacc ctagtagaac gatacagccc
    15241 acattatcct tgtgtttggc agctactacg gagtaatagc gtccatcttt ttcaaagagt
    15301 tttggatcac ggaaatcagc agcaatcaac tcatctggca agtctgctcc tgttgcaact
    15361 ggattttgtt caatcttttc aaagtgaatc ccgtcgtctg aaaatgccat attttgcact
    15421 tggcggacac cgtttttttc ttcgatatgc ccagtgtaca tgagccagag gcgatcatcc
    15481 ttgacaatgg cagagcctga gaaacaacca tttcggtcat aatcttgatc aggagcaagt
    15541 gccactggca agtgctccca agtcaccaag tccttacttt tagcatgtcc ccagtgcata
    15601 ggtccccaaa cactatcata tggatagaat tgataaaaga gatggtattc tccacgaaaa
    15661 taaacaaatc catttggatc attgatccaa ccaatctcag ctgaaaaatg ttcttcaggc
    15721 ttatattgag tattaacgag atgtttattt tctgctataa aacgattggc tttttctact
    15781 gtgtatttct tatctcccat aatcaacctc tagtttttgt ttgcttggta ttggtcgtag
    15841 tatttttgtt taatagcgag gtaatcagaa agtccgtatt tttcaagttc tttcttgtaa
    15901 tcatcccact cagtatcaat attgccattt acaatccatt cagcacgttt acggtagata
    15961 tagtcattca tatctgcttc gatatgggca atcttgtcca aatcttcctg tgtcataaag
    16021 actcttggat agttattgtc attgctcatg taaggaacat aatattcttt gataagatcc
    16081 aaacgccatt tggcatcatc aggcatggtt gttactttac catagtatga atctaggata
    16141 gctagtggtc ctcctacttc agtcttttga cgaagttctg ctggtgcagt tccgtttagt
    16201 ggtaagtgtt ttagactatt tgacgcttga tccaattcaa agatgttttg ttgtttgtca
    16261 tctccgtaag ttccccagtt attttgcaca gattggagtg gagcgtattg tgcatcaatc
    16321 catttagctg tcaattctag gtttttgttt acactggtaa taaccatctt gtcacgtgca
    16381 aatcccatac cgtttgtacg agctacgtgt ttttgaccac ttggtccagc aagtactggt
    16441 aaaacatcat aactttcgtt acttccagta acattattct tatcccatgt aaagtaaaca
    16501 ccaaatttct gatcatgacc tttagcaatg taactattcc aatcatgttc gaaagcttct
    16561 ttatcaatca agcctttttc ttgcaattga cggataaatt tgacaccttc tttatagtta
    16621 tcgttatctg ctgtgaaatc aactttgcca tcatttccta ctactaaatg atcatcgtta
    16681 tcccctatac caaatgcagc aaataggaat ttaaaatctt cgtttccgtt accactaata
    16741 aatgaaaatg gaatttcatc agcctctcca tttccatttg gatccccgtt tttgaaagct
    16801 tctaggactt taatcaaatc atcagtagtt tttggcattt caagaccaag tttcttaagc
    16861 caatctttgt taatccaagc catatcgttg acgctgtgaa tagactcttt accatctcca
    16921 agctcttcaa tccatgggaa tgagtaaatg tgcccatcag gtgctgtcat caaggccttg
    16981 tactctggtt tctcatccaa aattttctta agatttggca tgtatttatc aatcaaatct
    17041 tcaactggaa taataacacc ttttttagcc cagttcatca agtccacatc tgaagctccg
    17101 tcgttgtgga tagcatctgg taaatcacca ctagaaatat ccaagttacg tttttctgca
    17161 aagtcggatt ggtagttggt ccagtcaata tgaacgccag tttccttctc caaacgttgc
    17221 aaaattaact tttcatttgg gtctttagga gataacggtg aactggctgt cataaacttc
    17281 aatgttttct tttcttgaag cgggaatgtt acaccttcca acttataatc tggacttgaa
    17341 gctgtatttt ttgagccaca ggctgcaagt actgctaaac tagctgtcaa caaaactgct
    17401 gattttgaga atgttttgaa tttcatgaga aaactccttt tattttttct ttttttatcc
    17461 tttaagtgaa ccagccataa ttcctttatc aaagtatttt tggaagaatg gatacataac
    17521 aatcaatggc aagctggaaa tgacaatagt tgcgtattta atcaattcag ctaaacgttt
    17581 catttcattc atagccgctt gtgctccaat catgtcttga cctggttggc tctgaatgag
    17641 aattttacga agtacaagtt gcaatggttc caagtttgga tccttgatat aaatcattgc
    17701 atcaaagtat gagttccact gtcctacaaa agcataaagg aagagaacaa acataattgg
    17761 ttttgcaaga ggaagcatga ttttgaagaa aatctgtaaa tcatttgcac catcaatgac
    17821 agcagcttca actaattctt caggcaatcc ttggaaatag gccctagcaa gaataatatt
    17881 ccaaacattg acagcacctg gaacaatgat agcccatgga gtattgagca ttcccaattc
    17941 ttttacgagc aagtaagttg ggactaaacc accaccaaag aacatagtta caatcaagaa
    18001 gtagttaatc caacgacgtc caaccaagtc tttcttagaa agaggataag ctgtaaacac
    18061 agatagcaag actgttaaag ctgcaaatcc aaaagagtat agtagagaat tgataaaacc
    18121 tcttagaata gattggtcac tgaatacacg ctggtaacct tctacagtcc aatcggctgg
    18181 attaaagcta atccctctac taaccagaac cttaggatcc ataaaggatg ctacgacgat
    18241 ataaagtaaa ggaagcaaag tcatcaaaac gataaagaca ataatgattt tatttaagag
    18301 taagatacgt ctatcaaatt ttgtatccat aatcgaattt ttcatacttt cctccttaaa
    18361 ttccttcacc attattcatg cgtttaacga tttggttaac tgcaacaagc aatactacgt
    18421 taatcactgc attaaacaaa ccaaccgctg ttgagtaaga atagtctcct gatacaagac
    18481 caactttata gacatatgtc gagataattt cagaagttgg caaatttaac gatgtctgca
    18541 tcaagaatgc tttttcatat ccgacattca taattccacc tgcagctaaa acaaattgga
    18601 taaccataat aggcttaaga gctggaatat caatgtgcca gattcgttgg aagatattgg
    18661 ctccatccag tcgagctgct tctactaagg ctggatctac atttaccaat gttgccgtgt
    18721 agagcgttga agcccagccc attccttgcc agataccact aaagatgtat aagggtctaa
    18781 agaagtctgg atttgtcaag aagtcagcct tcattccaaa catagaaaga aaattgttga
    18841 ttggtcctcc cactgaaaag aagaggaaaa tcataccgac aataacaacg actgagataa
    18901 agtttggtgc gtataaaatg agctgaattc gttttttgac tttttcactc aagagttgat
    18961 tgagcataat cgcaagaatg attggtggta aaaagccaag caataaacca tagatactta
    19021 attttaatgt gttggctaac aagataccaa agttgggaga ggatatgaat tttgtaaact
    19081 cagaaaaacc aatccaatca ctccctaaaa ttccttttaa aggattgtaa tctttaaatg
    19141 cgattaaaac cccatacata gggatgtatt taaagataaa ggttaaaact aaaccgggga
    19201 cagtcatcaa taacaagagt ttttgtttct tgattctttc ccaaagagaa acttgcttcg
    19261 ctttgctatt catagcaacc tccttttttt tgaaacgttt cattttttct ataaaagata
    19321 gcgcttggcg ctgtcttttt atttcttttt ttgaaacgtt tcatttttgt tttaaaaata
    19381 aatcttttta tctgaattca ttctatcatt gatagcgctt tctgtcaaga cttattttca
    19441 ttttttgttg tttttccttc aacaaatttt actggtagga ttgtctgcaa attaacagtc
    19501 tttccatcaa ttatatcaaa caaaatacga accgcttctt ttgccatttc ttccaatggt
    19561 tgttcaatag ttgaaagtag ataatctcta tctccagcca ttttaattcc atcgtatcca
    19621 ataatttgga catcatgagg aatacgtttg ccattttttt ctagtatttc aatcacatcc
    19681 aaagctgtaa aatcgttgat tgtaaaaatt ccatctatag aagaattttc ttccaagaaa
    19741 ctctctagct ttccataata gtcagtaact gtctcatcca aatcaaaaat actaagaggt
    19801 attttatgct cttggacata cttttcaaat gaaattctac gacgtttggt ttcattaatg
    19861 gttgtattat gtccgcccac aaaggctaaa tgctgacatc ctttactaat taattgttta
    19921 gctgcttctc ttccacctgc atcattatct gatgaaacac aaggaatggc aatatctgag
    19981 tatgtgcggt caatactaac aaagggaatt cctgacgtca agtaatgttc aattggacta
    20041 taggtaatgg caaccactcc atcaacttta ttatgacgca acatctccag atagtcttgc
    20101 tctctatttg taccatttat agaacataag agtaatttgt tatttctctt atagacttca
    20161 ttttccacat gcatagcaaa ttctgaaaag aagggatgcc agatacttgg tacaatgatt
    20221 gcaatcgttt ctgttcgatt ttttttcatt cctctagcat agtaatctgg aatgtaattc
    20281 aaagttttaa tcgcttgttc cacttttttc aaagttactt ctttaatgcc tttttcttta
    20341 ttaattacac gtgaaacagt tccaacacta actcctgctt ctaaagcaac atctttcatg
    20401 gtaattgatt ttctttgttc taccatatta tcacctcctt tcaatatata gtatcatgca
    20461 aatgcttttt aagcaactat ttctcaatca tttttggcca gatcatttat cccatcatga
    20521 ataaaatcac tccaattagc ttttgaaaat acttcaattt tcatgtgtaa acatctacat
    20581 aaaacaggaa aagccttggt tttatggctt ttttcgtatc ttctacaaaa aaagcaagag
    20641 ttttagaggg ctataaatct agatgtacat tttgcttaaa tgattgaagg tcttttctta
    20701 acaaaaacac cccaaaaatt agattttttc tgtctaactt ttgaggtaca gttcaaacgc
    20761 gaaatagcgt ttttttgtta tttttggtta ctcatctaat cgaataaaca tcatggcatt
    20821 taacaagtat atgagtgaga ccgtgtttat attatttgaa tagatgagtc tcttattttc
    20881 aataggagga ataataaaat tagaaataat gatatcataa ggtgaatctt ctaaagattc
    20941 ctttgataat tctaattcag tccaaacttc cagttcaaaa ttattgctac agtaataaga
    21001 aagtgtctct gcaacgaatt ttgcatgata ctgatcaaaa ttactcataa ctaaaacctt
    21061 tagtttaggc tgattttgta gcaaattaat caccaaatgt ttggtatgag tgatgaaggt
    21121 ataagataga tgatttacca tcattgaact agaacaaacc tcaagagtct ctaaatagtg
    21181 agaaagctct ttttttatat ctgaaacaaa ttttggaaaa atattttgaa agttcctgat
    21241 tgtattccct ttttgatcaa ataaaataaa ctcagtaaac aactcttgac gatacagatg
    21301 tgcggtatta tgcagatgcc aaatcagatt atccttattc tccatttcaa tctgatactt
    21361 gactgaaatc tgatcaataa aatcactcaa tagatggtaa gatttttcaa cataactatc
    21421 cttttttacg catttcataa agagactttc atctatgaaa aacatttttt gaaagtaaga
    21481 cacaaataat tggcaaacaa cttcttcatc taaagagata ttgtattctg attcaaaact
    21541 ctgagcaaca ccttctattc cttctgcctg cattaaaaaa tccaaacttt ggtcgttaaa
    21601 agaatcttta tctacttcca taaaatgacc aaactttatt ctatataggt tcgtaactag
    21661 gagcaacttt agcattctat gcgttgacaa attcattgga aagcttgttt ccttataaac
    21721 caattctaac aattgagata gtggctctga tgaaaaattt tcaaatggcc attctaggaa
    21781 ataatatttt tctgaaaaat attgtgcaaa aaagtaacga atgtctctct catttccaat
    21841 gatttgaaca ggggtcagac tgacttcaaa ttgaaattgc cttttaatca ctttattgat
    21901 ttggctaata atacgataga gcgaagatga actgatataa aattctttac aaatactctc
    21961 agcttgacaa ccttcattaa agaagatgaa ttctaaaatc gaaaaatgag ttgaatgttt
    22021 aaagaaatga tggtaaacca tttcaatatc actatcatcg gtattaataa tgcgtatacc
    22081 attagtagaa gaatgaaaaa tcaagtcagg aaaagcagat ttaacatggg atagatcatc
    22141 tttgactgca cgttctgtac aatttaataa ctctgctagt tcagaacgat gaaaccaacg
    22201 tttatgttca aataataatt ctaataattc taattgccta tgactttttt tagataataa
    22261 atctctcatg aatatctttc tctctttata aattatcgga ttaaacctct tgcaattata
    22321 ccacaaagaa taggtatagc atgatataac gacttttcct aaaatctttt atttcgtata
    22381 ataacactac ggagacaata tataaacaat tttcttattt taccgtc
//
LOCUS       DAVKLX010000034        22161 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKLX010000034 DAVKLX010000000
VERSION     DAVKLX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22161
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.034"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(16..213)
                     /gene="blpI"
                     /locus_tag="VW325_001435"
     CDS             complement(16..213)
                     /gene="blpI"
                     /locus_tag="VW325_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEU9734810.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            495..1082
                     /locus_tag="VW325_001436"
     CDS             495..1082
                     /locus_tag="VW325_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU9734811.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            1009..2652
                     /locus_tag="VW325_001437"
     CDS             1009..2652
                     /locus_tag="VW325_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9734812.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2663..4024
                     /locus_tag="VW325_001438"
     CDS             2663..4024
                     /locus_tag="VW325_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9734813.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            4081..4209
                     /gene="blpC"
                     /locus_tag="VW325_001439"
     CDS             4081..4209
                     /gene="blpC"
                     /locus_tag="VW325_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9734814.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(4280..5620)
                     /locus_tag="VW325_001440"
     CDS             complement(4280..5620)
                     /locus_tag="VW325_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9734815.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(5634..6371)
                     /locus_tag="VW325_001441"
                     /pseudo
     CDS             complement(5634..6371)
                     /locus_tag="VW325_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(6376..6714)
                     /locus_tag="VW325_001442"
     CDS             complement(6376..6714)
                     /locus_tag="VW325_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9734816.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            6990..7319
                     /locus_tag="VW325_001443"
     CDS             6990..7319
                     /locus_tag="VW325_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9734817.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7487..8536)
                     /locus_tag="VW325_001444"
     CDS             complement(7487..8536)
                     /locus_tag="VW325_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734818.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8533..9264)
                     /locus_tag="VW325_001445"
     CDS             complement(8533..9264)
                     /locus_tag="VW325_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734819.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVADPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9332..9742
                     /locus_tag="VW325_001446"
     CDS             9332..9742
                     /locus_tag="VW325_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9734820.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9752..10039
                     /locus_tag="VW325_001447"
     CDS             9752..10039
                     /locus_tag="VW325_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734821.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(10729..11865)
                     /gene="dnaJ"
                     /locus_tag="VW325_001448"
     CDS             complement(10729..11865)
                     /gene="dnaJ"
                     /locus_tag="VW325_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9734822.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(12267..12623)
                     /locus_tag="VW325_001449"
     CDS             complement(12267..12623)
                     /locus_tag="VW325_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU9734823.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(12625..14448)
                     /gene="dnaK"
                     /locus_tag="VW325_001450"
     CDS             complement(12625..14448)
                     /gene="dnaK"
                     /locus_tag="VW325_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9734824.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(14928..15452)
                     /gene="grpE"
                     /locus_tag="VW325_001451"
     CDS             complement(14928..15452)
                     /gene="grpE"
                     /locus_tag="VW325_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9734825.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15479..16513)
                     /gene="hrcA"
                     /locus_tag="VW325_001452"
     CDS             complement(15479..16513)
                     /gene="hrcA"
                     /locus_tag="VW325_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9734826.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKTLQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16681..17187)
                     /locus_tag="VW325_001453"
     CDS             complement(16681..17187)
                     /locus_tag="VW325_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9734827.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCMSLFREDLSMSFFLIFCFICFSDFLSNSWLFQ
                     TKKEIPSR"
     gene            17795..20128
                     /locus_tag="VW325_001454"
     CDS             17795..20128
                     /locus_tag="VW325_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9734828.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            20141..21604
                     /locus_tag="VW325_001455"
     CDS             20141..21604
                     /locus_tag="VW325_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9734829.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            21604..>22161
                     /locus_tag="VW325_001456"
     CDS             21604..>22161
                     /locus_tag="VW325_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9734830.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQ
                     NKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
                     NIAVVRELKEYLIASTAFIVLDTLLN"
BASE COUNT         7049 a         4502 c         4080 g         6530 t
ORIGIN      
        1 ttaagaatca taatcttaca aagaggacat aattcctgaa cctacacaaa taagtgttgc
       61 tgctccccca gttatcggac cagtcgcagc agctaatagt actgctccaa tacaaccacc
      121 gattgcagat cctaaattgc ctcttcctcc actaactatt tcgagttctt cattatccat
      181 aacagaaaat tgttccatca tttttgtatt catgacaaat actccttttt tcttttttta
      241 tttttgtctt gttgtaactt tgataagttt agtatatcat cgttttttaa aatttttcat
      301 ccagatcttg aattgtcatc gaaacgtctt gaattaggtt ttttatttca agccacctct
      361 aaatgtttaa aaaaaataat ttctaatcac ttttttacca ttcaggaagt tttaatgact
      421 attcaagatt tcataaaata tgaacttagt tttatgacat aatagaccta tccactatat
      481 gaaaggaatt gccaatgact tcttataaac gtacatttgt tcctcaaata gatgcgagag
      541 actgtggtgt cgctgcctta gcctcgattg ctaaattcta tggttcagat ttttctctag
      601 ctcacttgag agaacttgca aagaccaata aagaagggac gactgctctt ggcattgtaa
      661 aagccgctga tgaaatgggc tttgaaacaa gacctgttca agcagataaa acgctctttg
      721 acatgagtga tgtcccctat ccatttatcg ttcacgttaa caaagaagga aaactccaac
      781 attactatgt tgtctatcaa acaaagaaag actatctgat tattggtgat cctgaccctt
      841 ctgtaaaaat cactaaaatg tcaaaagaac gctttttcta tgaatggact ggagtagcta
      901 tttttctagc taccaaaccc agctatcaac cccataaaga taaaaagaat ggtctactaa
      961 gcaagcttcc ttcctctgat tttcaaacaa aaatctctca ttgcttacat tgttctctca
     1021 agcttattgg tcactattat caatataggt ggttcttact atctccaagg aatcttggat
     1081 gaatacattc caaatcagat gaaatcaact ttaggaatca tctcagttgg tctggttatc
     1141 acctatatcc tccaacaagt catgagcttc tccagagatt atctcctaac cgttctgagt
     1201 cagagattaa gtattgatgt gattttatcc tatattcgcc atatttttga acttcccatg
     1261 tctttctttg cgacacgtcg tacaggagaa atcatttcac gattcacaga tgctaactct
     1321 attatagatg ccttggcttc taccattctt tctctttttc tggatgtttc tattctgatt
     1381 cttgtaggag gcgtcttact ggcacaaaac cctaatctct tccttctttc tcttatttcc
     1441 attcctatat acatgttcat catcttttct tttatgaaac ctttcgaaaa aatgaaccat
     1501 gatgtcatgc aaagtaattc tatggttagc tctgccatta tcgaagatat caacgggatt
     1561 gaaactataa agtcgctcac gagtgaagaa aatcgctatc aaaatataga cagcgaattt
     1621 gtagattatt tggaaaaatc ctttaagctc agtaaatatt ctattttaca aacgagttta
     1681 aagcagggaa caaaattagt tctgaatatc cttatcctat ggtttggcgc tcaattagtc
     1741 atgtcgagta aaatttctat cggtcagctg attaccttta acacactttt ttcttacttt
     1801 acaactccta tggaaaatat tatcaacctc caaaccaaac tccaatctgc gaaggtcgct
     1861 aataaccgtt tgaacgaagt ctatctagtc gaatctgaat ttcaagttca agaaaaccct
     1921 gtccattcac attttttgat gggcgatatt gaatttgatg acctttctta taagtatggt
     1981 tttggacgag ataccttaac agatattaat ctcacgatta aacaaggaga taaggttagc
     2041 ctagttggag ttagtggttc tggtaaaaca actttagcca aaatgattgt caatttcttt
     2101 gaaccctaca aaggacatat ttctatcaat catcaggata ttaaaaacat tgataaaaaa
     2161 gtcttgcgcc gtcatattaa ttacctacca caacaagcct atatctttaa tggctctatc
     2221 ttggaaaact taactttggg cggtaatcat atgattagcc aagaagatat tctaagagct
     2281 tgtgaattag ctgaaattcg tcaagacatt gaaagaatgc ctatgggcta tcaaactcag
     2341 ctctctgatg gagctggtct atcaggagga caaaagcaac gaatagccct cgctcgtgct
     2401 cttttaacta aagctcctgt tttaatacta gacgaagcta ctagcggtct tgatgtcttg
     2461 actgagaaaa aggttataga taatcttatg tctctaactg ataaaaccat tctctttgta
     2521 gcccatcgtc tcagtatagc cgaacgaacc aaccgtgtca ttgttcttga ccaggggaaa
     2581 atcattgaag ttggtagtca ccaagagtta atgcaggcgc aaggtttcta ccatcatcta
     2641 ttcaataaat aaggagaatg tcatgaatcc taatcttttt agaagcgtcg agttttatca
     2701 gagacgttac cataactatg cgacagtgtt cattatacct ctttcattac tatttacttt
     2761 catcttgatt ttctcccttg ttgccacaaa agaaattact gttacttccc aaggagaaat
     2821 cgcccctaca agtgtcattg cctccattca gtcaaccagt gataatccta tcctagctaa
     2881 tcatttagtg gcaaatcaag tagttgaaaa aggggactta ctcatcaaat actctgaaac
     2941 aatggaagaa agtcagaaaa ctgccttagc aactcaatta caaagacttg agaagcaaaa
     3001 agaaggactt ggaattttga aacaaagctt agaaaaagcg actgatcttt tttctggcga
     3061 ggatgaattt ggctaccata atacctttat gaattttact aaacaatccc atgatattga
     3121 actgggtatc acaaagacta acaccgaagt ttcaaatcaa gctaatcttt ccaatagcag
     3181 ttcatcagct attgaacaag aaattacaaa agttcaacaa caaattggag aatatcaaga
     3241 gttgagagat gctatcataa ataacagagc acgcttacca actggcaatc cgcaccagtc
     3301 aattttgaat cgttatcttg tagcctcaca aggacaaaca caaggaactg cagaggagcc
     3361 atttttatct caaattaatc aaagtattgc aggtcttgaa tcatctatcg caagcctcaa
     3421 aattcagcaa gctggtatcg gaagtgtagc aacttatgat aacagtttag caaccaaaat
     3481 tgaagtactc cgcactcagt ttttacagac agcctcacag caacaactaa ctgtggagaa
     3541 tcaattaaca gaattaaaag tacaactaga tcaagccaca cagcgtttgg aaaacaatac
     3601 cttaacctcc ccaagtaaag gtatcgttca tctgaacagc gaatttgaag gtaaaaatag
     3661 aattccaact ggtacagaaa ttgctcaaat attccctgtc atcacagata caagagaagt
     3721 actaatcact tactacgtat cttctgacta tctacctcta ctagataaag gacaaactgt
     3781 aagattaaaa ctggagaaga ttggaaatca cggcaccacc atcatcggcc aacttcagac
     3841 aattgatcaa actcctacca gaacagagca aggaaatctc tttaaattaa ccgctcttgc
     3901 aaaactatct aacgaggata gtaaactcat ccaatatggc ttacaaggtc gcgtcactag
     3961 tgtaactaca aagaaaacat attttgatta tttcaaagat aaaattttaa cccattctga
     4021 ttaattttca gataacactc tataactatt tattatctta tcaaaaagga gaatcataac
     4081 atggataaga aacaaaacct aacttcattt caagaactaa caactaccga actcaaccaa
     4141 attacaggtg gaggattgtg ggaagattta ttatataaca ttaatagata tgctcattac
     4201 atcacataag aacttcatca tccaatacaa ctataaaaaa ataagaccga gaaacaagta
     4261 ctctcggtct tatttttcat cattctgtat gtatcacagt aagtacctga cgaaagactt
     4321 gattttgaca ggtggtattt agactggtat taggatggct ttccacaatc ttcatgacgg
     4381 tatagagacc aactcctctc tcctcccctt tagaactggc tccaaaggag aagatttcag
     4441 aaatatcgat gccctcttct ttgatggagt tttcgatgat aaaggtctcc tgtgctccat
     4501 tttttaaaaa ggcgattgaa acatgaggtt gactagcttc cacactggct tcaatagcat
     4561 tgtcacaaag gatagacaca atggttagaa aatcaagtag actcatcccc tcgacctgaa
     4621 tctcctcagg aacttcgaca ttaaagacaa tgttcttatc tctggctttt aaaaatttcc
     4681 ctgctagaag acttttgagg gctttatcac gaatatttac caatctgccc aggtcatatt
     4741 tattgttctg caatttctga ctggaatcct ttaagacgga gccatagacc tcttttatct
     4801 gctccatatc ctcctcttca atgcccagac gtaagctagt caagaggttg gtataatcat
     4861 gacgaaagct ccgtacttcc ttgtaaagct cctctatatg ccgactatag cgttccatat
     4921 ctctatagcg cagggcctgc tcttgttcca atctctcata gagtttttcc ttcaaatagg
     4981 tatccaattt cttgataacc cccataaaaa agagtaggta aaagactagg atgagatggc
     5041 gaacagtctt tgattgaata ctttgttcat attcaaaaaa agacagactt tccatgacta
     5101 gatagtagcc acccattatc cagttaatct gagtcaggga cttttgaaag gctttatcga
     5161 gaatctcctt tctcaagcta gtaaaatcgt agtccaacca tttcaaaaaa gctagagaaa
     5221 tgaagaaatt gaaaattatt atacataacc cagtaaatga gtagccatca tatacttgcc
     5281 cttgtcccaa aaatggaagc acaaaatagg agactcctct ataaaagaga ttcaccaata
     5341 tcattggaaa gagaccataa aagaaaagga gttttttagg aagccctctc aataataaga
     5401 aagataagcc tatgccgtac aagggttcca taaaataaga taggtaaaca tttcctacta
     5461 tatagctaat catcacaaaa acaaaggcca acagtatctt aaaaagaaag gccttaaaaa
     5521 tcctctcgaa agtaagatca attccatcca ccttaaagaa gatgacaatt tctagtccat
     5581 tagtaacaag tgtatacaac aatatccaag caatgttcat aaattctcct agctcagtgt
     5641 aatttattga tggcctcaga cacttccctg accttataac gggcgattag acaacttcca
     5701 ccattgggaa agaaaagcag tttttctttc ttatccaaat gcaccacatt tgcaggattg
     5761 atgagaaaag agcggtggca ctgcaagaga cggggctcct gcttgaaaac ctcctctaaa
     5821 ctcgctgtaa attccagcct gtctgtcttg gtatagagaa taacacgatg ggctctgggc
     5881 gacgtttcga gatagtaaac ctctttaaaa ggatactgaa attgggcaaa ttttgattta
     5941 aagtaaaagc aatcttccgc cagactttta ctatcttgac tattggcata gaggagggct
     6001 gtctcgatcc gagattcaaa ctcctctgct gacaaggcct tatcaatgta gtccaaagca
     6061 gacacttggt agcgaaaaga caggggcata aactccgagt gagtcgtcac aaagacaatc
     6121 agggcataag gatcccgatc ccgaatcttt ctagccactt ccagtccctt catctcttca
     6181 tttcgaatct caatatccaa aaagaatagc tgatgggccc ccttctcatg cacttcagcc
     6241 agcagttggt ccggcttgcc aaatacctca aaagagctag gaatgatatg atgtgctttc
     6301 aaaagtttct caatcgtcgt ttcaattcta gtctattggg aaaaatcatc ctctaaaaca
     6361 aatattctca tcttcttact ctccctcttt caaccatttt tgacgaattt ctctatagcg
     6421 acgtctggca tacttgacag cgtattgacc ttcttctccg agaaaaagga ttctttcttg
     6481 aaaatcaatc gattttaatt tatcaagatt aaccaaacaa tttcgatgac atctcatcaa
     6541 ggtttcccca cattggttct caagattact taaattttga agcatattaa aactagcttc
     6601 ttctgtaaca atctgtacgg tatgggcttt agttggatgt gtttggatat agtagatatc
     6661 gtcgatgttt actttataga ctgttttctg cgtctgaata atcatatatt tcattggatt
     6721 ctcctcctaa gaaaagaata acagaaaagg tctagcccaa gcacacccaa tcctaaacag
     6781 tctttgaaaa ctcctaaaag gattattttc tacaacatca tgatgagctt acaactattt
     6841 tatcataaaa ttcactcagc gcaattcaag acatttcaat gacaattaaa gatttgaata
     6901 aaaaaaaaga attaaaagtg ataaactaac agtatcaaaa cacagttgcc agaagtaaga
     6961 ctggcaccca gattaaagga ggcattccca tgacagatac agaccccatc aaaagagctc
     7021 atactttgat tactgactta aacaaagcct accaagcatg caaacaggca agcgctgacg
     7081 acgtccgctt tcaggagcaa ttaaactcta ttcttggttt tctagccaag gctgaaacag
     7141 tggataatcg attcttgatt gaactagaaa aattttacca gacttccagt cttctcatgg
     7201 gactcagcgc tcttgatcca gatgctccaa ctcgcgccgc ttggcgggcc tatgaccgct
     7261 tccactttga ccaagtcaag accaagttaa tactcaatga aaatcaaaga gcaaactagg
     7321 aagctagccg caagctgtac ttgagtacgg taagacgacg ctgacgtggt ttgaagagat
     7381 tttcgaagag tataagtctc tacggaccaa ccattatcct gtagtaacaa aaaagaagtt
     7441 gagacagact gaactcaact tcttttttag tgtcgtatca gcaatgttag tcctgcatct
     7501 gacgttttac ctgataaggc aaatacaaga ctagtaaaac caaaccagct cctagtaagg
     7561 ctagaagggc tagtttttct tggaaggtaa tcaacccaac aactaattcc acaagtaaaa
     7621 caaaactggt caatcctcga actacctcct gtaagccttt ttccttttgc cccttgtcca
     7681 gcggaaagag ttgggtcaaa tactggtagt caaaggcatg atagagggcc agcaactgga
     7741 agagcaagag gtagttaaag agaactacca ctgctgtcgc aatccaagct tgctcgataa
     7801 aaacctgcgc cagtacggaa agtaagagaa gacgaagact gagagcaaag aggtcgccat
     7861 ttcgcagata agaacgcaga tagagatttt gccaaatctt cccaggcacc ttctgaacag
     7921 cctttaagat aaagtccaga taggcacgac gcttaacgct gtttgaaatt cccttgacct
     7981 gcgtaaagag ggcaaagaaa cgaagcaaga cttgcttacg cttgctttct tgggaaatga
     8041 catagtccca atccagtcca gtttcagtga aaaatttgct ggccttttga cgaaagtgga
     8101 aatattttcc tacccccaat aaaagcacat agagcagaaa aactggcaag ccataaccca
     8161 ttgctaaaaa taaaggcgca aataacagca agaaaagggt ctgtacaaag agccaaaaga
     8221 ctagggaaat gccagtttga cgcttgagat ggagcttgat ttcctcttct ccaactaaga
     8281 gaaagagctt gtctggagcc tccatatagg tggcaattcc tccccaaagt aaaagtaaaa
     8341 cagacgtaat tcctacaaac aaaaggatag gccaatgatt ttcaggaaaa tgttgtaaga
     8401 gttgactgta ctggtaggct agaaagccca acaggacaag caggaacaag acaaagtggt
     8461 cattgagcac atagcgcaga taaccaagac actccttacg aaaggcctgc tttctcttta
     8521 aaaacaagtc tttcataggt cctcctcttt tgtcagagcc aagtaaatat cattcaaact
     8581 agcctcaggc atatcaaagg cttcgcgtag ttgcaggaga ttgcctttgg cacgcacctc
     8641 tcccttgtga agaatgacaa aggcatcaca catcttctcc gccgaatcca gcacgtgggt
     8701 actcatgaga atagacttgc ccttttgctt ctccacttcc aaaagctgaa tcaaatcaga
     8761 aatagccagc ggatcaagac caaggaaagg ctcatccacg atgaaaagac ttggatccgc
     8821 cacaaaagca cagataatca tgaccttctg cttcatccct tttgaaaaat gaacagggaa
     8881 ccagtctaat ttctgttcca aacggaacat ttttaacaag ggctctactc gttcgaaagc
     8941 cactttttgc tcaataccgt aagccatagc aaccgtttcg atatgctctc tgagggtcaa
     9001 ttcctcatac agactaggcg tctcaggaat gtagccaatc tgcttgcggt agctagtcgc
     9061 atctccttgc agagtcaggc cattgatatt gatggagcca ctataaggtg ccaacagacc
     9121 gataatctca ttgatcgtcg ttgatttccc agcaccattg agaccaatca aaccgaccaa
     9181 ctgcccactt tcaacagtaa aggacacatc tttcaaaaca ggaacatgaa catagccacc
     9241 tgtcaggttt ttaatttcta acatattttc tccaaatctg gtataatgta gctatattat
     9301 atcaaaattc agtacagtag aggtagattt tatgtcagat tgcatttttt gtaaaatcat
     9361 cgcaggggaa attcctgctt caaaagtata tgaagatgag caggtccttg cctttcttga
     9421 tatctctcaa gtaacactag gacacacctt ggtcgtgcca aaagaacact atcgcaatct
     9481 tttggagatg gatgctacga gcgccagcca actctttgcc caagtaccaa aagtagctca
     9541 aaaagtcatg aaagtcacta aggctgctgg tatgaatatc atttccaact gtgaagaagt
     9601 cgctggtcaa acagtttttc atactcacgt tcaccttgta cctcgctaca gtgctgacga
     9661 tgacctcaag attgatttta tcgcccacga accagacttt gacaaacttg ctcaagtcgc
     9721 tgaaaccatc aaaaacgctt aaggagttgc tatgaaatta tccaacctac tgctatttgc
     9781 aggagctgca gccggaagtt atctggtcac aaaaaatcgc caaaccatca cagatgaagt
     9841 cttgaatacc actgaccgcg ttcaagctat caaggacgat gtggatatta tccaaaacag
     9901 cctgcaaatc attgaccagc aaaaagaact tatcaaggaa taccaagaag acttgactta
     9961 caagtttaag gtcttggaaa aggatatcca gactagacta gctgtgataa aagaaatgca
    10021 gggaactgaa gataagtaaa aagagcccga cggctctttt ttaaaacgta aacaagttat
    10081 agtgaattga atttagaata gcacatcata gtttctaaag cattgttaga gattacttta
    10141 aaatcctctt atcaatttgt tcatattcta tttcaatcta ctatatatat cccatcaact
    10201 atgcatcata atttaggtaa ctcatactca ataaaaatca aaaagcaaac tagaaagcta
    10261 ggcacagact gctcaaaaca ccgttttaag gttgtggata gaactgacga agtcagtaac
    10321 catacccatg acaagccgac gctgacgtgg tttgaagaaa tttttgaaga gtatcattca
    10381 ccatttcaca actaaatcga agactttctg ccgtaatgaa aacacctgaa acagcttggt
    10441 ttcggctatc cggaaacttt aagacttagg ttcaaagttt aggtatggaa cttcgaagga
    10501 ggtcgctacc gtccgtcatt acttaggaaa gtcttaaaaa atctataaag taaaaagagc
    10561 ccgacagctc tttttgttta tactcaatga aaatcaaaaa gcaaactagg aaactagcca
    10621 cgggctgctc aaaacactgt tttgaggttg tagatacgac tgacgaagtc agtcacatat
    10681 atacggcaag gcaacgctga cgtggtttga agagattttc gaagagtatt attctccatc
    10741 aaaggcatct ttaatatggt caaagaagcc ttttttcttt ggatttactt tcaagtcacc
    10801 agcagccgcg aattctttca aggctacttt ttggcggtcg ttcaagcctg tcggtgttac
    10861 gacattaaca gtaacgtatt ggtcaccaac tgcaccgcca cgaaggctcg gtgccccctt
    10921 actacgtagg cggaacttct taccagtctg agttccctct ggaataacca attcaacatc
    10981 accgtgaaca gttggaatat ctactgtatc accaagagcc gcttggacaa agttgaggtt
    11041 gagattgtag aagatagtcg ttccttcacg ttcaaacttg tcgctagctt ccacagaaac
    11101 tactacatac aagtcaccat aaggtccacc gttaaagcct gcttcacctt gaccagcgag
    11161 gcgaatttgt tgacctgttt ccacaccagc agggattttc acatgtacgc tatgagcttg
    11221 tttctcatga cctgttccat gacaggttgt acatggatat ttgatttctt ttcctcgacc
    11281 gtgacagaca tcacaggtta cttggcgacg catcatacca agaggagtct gcgtatcgac
    11341 gttaatgaca ccagcgccat gacagcgtcc acaagtgact ggacttgtcc ctggcttagc
    11401 accagatcca ttacatgtac gacagccagc ttcacgatga tacttaactt ccttctcagt
    11461 tccgaagata gcttcttcaa aggtcaaatt gacacgatac tggagatcat ctccttggcg
    11521 aggagcgttt ggattgcgcg aagaaccgcc tccgccgaag aaacttgaga aaatatcctc
    11581 aaaaccaccg aagccacctg ccccattgaa accgccgaaa ccaccagctc caccaaaacc
    11641 accattggcg cctgcagcac catactggtc ataggcagca cgtttttggt cgtcactcaa
    11701 agtctcatag gcttcttgaa cttccttgta cttgtcctca gcaccaggct ccttgttgat
    11761 atctgggtga tattttttgg aaagcttacg ataagccttt ttgatttcgt ctgccgaagc
    11821 gtttttggat acccccagac gatcataaaa ttcagtattg ttcataattc aagatacaaa
    11881 gggcgtcaaa cggacacagc catctaggag ttttgacgac ggacgcttac gtcctagaaa
    11941 aaactatctt tttggcacag tccatagccc gtgttcagtt acgaacaccc ttattccttt
    12001 ctattgatgt tctgaatcga accaccattt aggtcgggtt caattccttt cttttatatt
    12061 tagtaagaaa ttctgaaacc cgtgttcagt tacgaacact ctttgttcct ttctattgat
    12121 gagacaaaac ccaaacaaat gagattccgt ccctttttat cgaaaaacag aggctgggtt
    12181 gcaacctctg caaataagta aattaacttt attttgattt taaatatcct ataagtacag
    12241 aaatatttat atatttttgt ctcttcttag tatttgccta tataattcca attgatcata
    12301 agaaatacaa ctactgtcca aatcatacca attatgaacg gtattacaaa tttatatgtt
    12361 tgtatttcta atgatatcat agctgatacc atcgctgata caaatgcaga caacaatact
    12421 gaagtaacaa aattccttac cgcttcatca tttttgacac aactatcatg ctcagctaat
    12481 ataatttctc tatatatagt caaacattct actggaatat tatctataaa accttttgct
    12541 gcttcatagt tgtcagcttg attacaaaaa tcattataaa cttttcgtgt attttttaga
    12601 tactcatcat ccaatacact catcttattt ttccgtaaac tctccgtcta cgacgtcatc
    12661 gcctgcgttt cctgttgctt gtgcgccttc tgctccttct tgagcttgtt gcgctgctgc
    12721 ggcttgttcg tagagtttca cagcaaggcc ttgagctttt tcgttcaacg cttcaagttt
    12781 cgctttcatg tcgtccaagt tgttgtcttc ttgagctttc ttaaggtcat caagggcagc
    12841 ttgggcagcg tcacgttctg cgtcgaagcc tttaccttca gtttccttga ttgtcttttc
    12901 agtcgcaaag attgcttggt ctacttcatt acgaaggtct acttcttctt tacgtttctt
    12961 atcggcttca gcgtttgctt ctgcatcttt catcatgcgg tcgatttctt cgtcagtcaa
    13021 acctgagttc gattggatga caatagtttg ttctttttga gttccaaggt ctttggcctt
    13081 aacagacacg ataccgttct tgtcgatgtc aaatgttact tcgatttgag gaattccacg
    13141 aggtgcagct gggatatcag tcaattggaa gcgtccaaga gtcttgttat ctgctgccat
    13201 tgggcgttca ccttgaagaa cgtggatatc aacggctggt tggttgtctg ctgctgttga
    13261 gaagacttgt gatttagatg ttgggattgt agtgttgcga tcgataagtt ttgtaaatac
    13321 tccacccatt gtttcgatac caagtgacaa tggcgttaca tcaagaagga caacgtcctt
    13381 gacatcacca gtaatcacac caccttggat agccgcaccc atagcaacta cttcatcagg
    13441 gtttactgat ttgtttggtt ctttaccagt ttcagcttta acagcttcaa caacggcagg
    13501 gatacgagtt gaaccaccaa caaggataac ttcgtcaatt tctgacaagc tcaaacctgc
    13561 atctgaaagg gcttgacgaa ctggaacttt tgtacgttca acaaggtcac gagtcaaatc
    13621 gtcaaatttc gcacgagtca aagtcatttc caagtgaaga ggtccagcct cacctgcagt
    13681 gataaatggc aagctgattt gtgttgaagt tacaccagaa aggtctttct tcgctttttc
    13741 agccgcatct ttcaaacgtt gcattgccat cttgtcagta gacaagtcga taccgttttc
    13801 tttcttgaat tctgctacca agtggtcaat gattttttgg tcaaagtcgt caccaccaag
    13861 tttgttgtcc cctgcagttg acaatacgtc gaagacaccg tcacccaatt caaggataga
    13921 gacgtcgaat gtaccaccac caaggtcaaa taccaagatt ttttcttctt tgtcagtctt
    13981 gtccaaacca taagcaagag ctgctgcagt tggttcgtta acaatacgtt ctacttcaag
    14041 accagcaatt ttaccagcgt cttttgttgc ttgacgttga gcgtcgttga agtaagccgg
    14101 aactgtgata acagctttgg ttactttctc accaaggtag tcttcagcgt agcctttcaa
    14161 gtattgaagg atcatagctg agatttcttg tggagtgtat tcttttccat ttgcagaaac
    14221 tttttcagaa gttcccatct tagatttgat agagataact gtatctgggt ttgtgactgc
    14281 ttgacgtttt gcagcatcac caacgatgat ttctccgttt ttgaatgaga ctacagatgg
    14341 agttgtgcgg ttcccttctg ggtttgcgat gattttgctt tcagttcctt caagaactgc
    14401 aactgctgag tttgttgtac ctaagtcaat accgataatt ttagacatgt gtttttctcc
    14461 ttgttagtta attttctatt tttctttttg atactcttct taagtggcga cgccgtcaga
    14521 gcttgacttc gtcaatctct ttgactaaac tttgagccta aggtctcaaa atttgcgcaa
    14581 atagcaccac ggcgaagagt atcgtctttg gttcgcttcg ctcactatta caaagccaaa
    14641 catattttat ctttcttcat agtttattgt cgtttcggac aagttttcta ttatgtaaat
    14701 tgcgacacga ggagtcgaaa tcgattttat ttcgacgacg agttagtaag gaagctaggc
    14761 aaacgtcata gcgattgccg ttttctgacg agttgcttta gctaccgtca gaattgccga
    14821 atcgaacacg ccctaagctc tgagtaaaaa agatagattt tcttgtgttc atcgaacact
    14881 tcgtcaatct cctattttta ctgcgagctt tttacgagct ttgtatctta gttatacacc
    14941 actaccattg ctgggcgtag gatgcggtca tggagtttgt agcctttttg aaagacttgg
    15001 gcgatggtat ctactgggtg ttcatcgtct gctgggagag tttggatggc catatggtag
    15061 ttatggtcaa attcgccatc tgctgcgatt tcttcaattc cttcttcttt caaagcgtga
    15121 atcaagcttt cttgcaccat cgccaagccc ttcttcacat catctgtcaa accttcaact
    15181 gcaagtgcac gctcaaggtt gtcaagagat ggtaagattg cttttgccaa gtcctggcta
    15241 cgataacgtt gcaagttttg acgttcttca ttggcacggc gttggatatt ttgcatttct
    15301 gcatgagcgc gaagatattt gttttcgaac tcatctgcac gttcatttgc caagtccaac
    15361 tcagactttt caggagttgt ttcttcagct gtttccacaa cttcctcttc ttgaacttct
    15421 tctacttctt cattttttat atcttgggcc atgttcgcct cctttaatga tttcaatctt
    15481 aatgtacttc gtaatgatta ctgctgaggt agcggtaaaa atctgtcaac ttcatggtca
    15541 aaacacggtt gaccacattg acttgattga ttagctgttg gtaatccaga ttaactggac
    15601 cgataatggc tagaattcca actccccgat aaggaatgag gaacttacta ctaatcaccg
    15661 ctaggtcagc taaacaggac tcttgaccgt ctgcaacacg aacattttgc atctgatcct
    15721 cacgcaaccc ctcacgaatc tccaaggcca ccttttgcgg ttggtcaaag aactgatagg
    15781 ctgctagatt ggcaaaattc aagagatgga ccttgcccgc catcacaatg ttttcgttga
    15841 acatttcctt aaagatgtgt tcaaagagat cgatgacatt atccgttgtt gtaaagtaac
    15901 gctggataat ctgcggaatc tccgtccgaa tcttgtagtg aatatctaaa acggtgtgac
    15961 cgaggaaacg ttcctgaatg atgctcttca gtttcagcaa atcctcctgc aagaagttcc
    16021 ttggaatcag aaactgacta gtaaccgttc gcgactcgtc tagggtaaat accgccaagg
    16081 ctgtatgttg ccccaaaaca acgatatcaa aggctgtcaa acgttgcctg ctcggctcaa
    16141 catccagtgc cactaccgta cagccactca ggtctgttag caagttagca gcctcttgca
    16201 gaatatcctc caatttgaag aattcctgat caaaggcttt gacaatctca tatacctcat
    16261 tttcagccag tcggtcaaaa tccagtgagt gtttcacata gtactgaaaa ccagcaacac
    16321 ttggcatccg accgcttgaa gtatgagcct tctcaagcaa cccttgtttt tctagttccg
    16381 ccatgtcatt acgaatggtt gcactgctag agttaataga ctcttgcaag gtttttgatc
    16441 cgacaggttc gtgcgttttg gtaaagatgt caataatcag atttaaaata tcctgctgac
    16501 gctctgtaac catctcatca ctcctctctg tctgatcaat tagcactcaa tgcatccaag
    16561 tgctaactca tgattctatt atacaccctt agaagagaat gtcaagacaa aaactcaaaa
    16621 aattagcact cttttatcaa gagtgctaaa aatcaatctg aagacctaga accttgctat
    16681 tcatctactc ggtatttcct ttttagtctg aaataaccat gaattagata agaaatcaga
    16741 aaagcatata aaacaaaaaa taagaaagaa agacatagaa agatcctctc taaacaaact
    16801 catacaaaca acaagaccac ctaagaaagt agctgtacag gaacgaagtc tagatcgcat
    16861 aacttcccat ttgagcccct tactcatata gacttgatcc tctggaagta aatcagaaga
    16921 cgatttacga agaatcgaac aagaacctgc tcctatcaat tcccaacctc tatctctaaa
    16981 atcttgcagt tcacgcttat gctctttaag aaatctagaa tcatagatac gatagataac
    17041 atcgtccggt tgaccttgct caaaatagaa caaaccaaaa cgactcgttc tatacctcca
    17101 acctttcaaa tgcatctcat gtaaatattc ttctttcttg tccaaatcaa caatggtgaa
    17161 aatccgaaat tctactctgc tattcattgt cttaccccaa aattagaaaa catgcctggc
    17221 gttatttatt agataattct ttccactttt gactcaatct ccaaaaaata taagaaatct
    17281 gaatcgcaaa aactatcaat aaaacccaat ctattatgaa aatcaaaaac actttccaac
    17341 tgaaagaact acctccagta acaaactttg agaaaaacgg tagtagagct aaaaagagaa
    17401 gtaaaatagg aagcatccgc attgttaaaa tccgtttggc ataaaaaaat ctttatttaa
    17461 actaaaatat tatagcaaaa tttacgccag tttttgaacg gctgatgtag atattttata
    17521 ctttcaaaat gtttaaatgt gattatttat ttttgaaaaa tagctcacca gcccgactga
    17581 aagtgcttat agaatgataa taagtcgcct gccgaaaaca gcggaaaata gcggtgttat
    17641 gcggagataa tctgacgcga tgcgaaagta tattgcatac ttattttcaa caatttagca
    17701 gagtattttt ataagtgtga tataatagaa gtataatttg ttctgatagt ttattttatg
    17761 gagaagtaga tttttagaat gcggagggtt caatatggtt gagtttataa agtctaagaa
    17821 agaaatgagt gaggaggata ttaaagcaaa tttcatcact cctgctattg tatccaaagg
    17881 atggaaaaat ggtgagcata tcgcttacga agaatacttc actgatggtc gaattgaagt
    17941 tagaggagat aaggctcgtc gtaaagaagg aaaaaaatca gactattcac tgtattacca
    18001 atttggaact cgaattgcaa ttgttgaggc aaaggataat aaacacagcg ttcgagcagg
    18061 attacaacaa gctattgaat atggagagat tttagatgtt ccatttgttt attcttcgaa
    18121 tggtgatggc tttattgaac acgaccgtat cacgagagaa gaacgtgagc tggagttaga
    18181 cgaattccct actcgtgaag aattattttc tcgtatgacg aaggaaaaag gattgacgta
    18241 cgaaattaca gaagctatct caactccata ctatacagac gccttctcaa tgaaaacgcc
    18301 acgctattat cagcaaatag ctatcaaccg tactattgaa acagttgcca gaggacaaaa
    18361 acgagtaatg tttgtgatgg caacaggaac ggggaaaacg ttcatggctt ttcaaattat
    18421 tcatcgcctt cgaaaagctg gtttggctaa acgagtttta ttcttagcag atagaaacat
    18481 cttagtagac caaacgatgg ctgaagactt taggccattc gaaaaggtaa tgacgaaaat
    18541 tacaccaaaa cttttgactg ctcctgaaaa attaaattct tttgaaattt atctagggct
    18601 ttatcagcaa ctaactggtg aagatggaac tgaaacacat tatcaaaaat ttgacaaaga
    18661 cttctttgat ttaatcgtaa ttgatgaagc acaccgtggt tcagctaagg aaaacagtaa
    18721 ctggcgtaag gtaattgatt atttcagttc tgcgacacag attgggatga ccgctactcc
    18781 taaagaaacc aagaatgctt ccaatacgga atactttggt gagccaatct atacttatag
    18841 tttaaaacag ggaatcgagg atggtttttt ggctccatat cgtgttatga gggttaattt
    18901 agatgtggat gtggatggtt atcgtccaga aactggaaaa gttgatgcta acggacaatt
    18961 aatagaagat aggtactacg gcaggaaaga ttttgataaa accattgtca ttgatgatag
    19021 aacgcaaaga gttgccaagt ttgtttctga ttatatgaag caaaacaatg cacgatttga
    19081 taaaacaatt gttttttgtg ttgatattga ccatgccgag cgaatgcgtg ctgcacttgt
    19141 aaaagagaat ctagacttag tccaagaaga ctatcgttat gtcatgcaag taactggtga
    19201 caacgctgaa ggaaaagctc aactggataa ctttatggat gtcaattcta attttcccgc
    19261 tattgtaaca acgtctaaat tattaacgac aggagttaat gctaaaacat gtcgtttgat
    19321 tgttttagac tctaatatcc aatccatgac tgaatttaaa caaattattg gtcgtggcac
    19381 acgtctttat cctcaaaagg ggaaagaatt ttttacgatt attgattttc gaaatgttac
    19441 caatttgttt gctgaccctg attttgatgg tgatccagtg aaggtgctgg aaacaggtgc
    19501 gaaaacagtc agtggttcta cgcccggttt cgtagatgag gaaggtgacc cagtagaaaa
    19561 atatatcgtt acagacaagc aggttaccat tcttaattct actgttcaag tattggatga
    19621 aaacgggaaa ctgattaccg aaagcctgac cgactacact cgaaagaata tcttaggtag
    19681 ctacgccact ttgaacgatt ttatcacagt ttggcatacg gcagataaaa agaagcttat
    19741 cttagacgaa ctttataaaa aaggagttta tctagatgct attcgagagt cggagggaat
    19801 atcagaacaa gaaatcgatg attttgattt actcctaaaa cttgcctatg gtcaaaaaga
    19861 attaaccaaa acggaacgta tcaataaact caaacaaagc ggatatttat ataaatatag
    19921 tgaggaagcg cgtgctgttt tggaaatttt actgaacaaa tacatggata aaggtattgg
    19981 agaactcgaa agcattgaaa cattaaaact tccagaattt cagatatatg gtggaacctt
    20041 caaaatcatc aatacttatt ttggagataa aaaacgatat ttacaagcaa ttaaagaatt
    20101 ggagcaagag ctatttacag tagcttaatg aaaggaaagt atgtcaatta catcatttgt
    20161 aaaaagaatt caagatatca ctcgaaacga tgctggtgtt aatggtgatg ctcaacgtat
    20221 tgagcaaatg tcttggttat tattcttaaa aatttatgat agccgtgaaa tggtttggga
    20281 attagaagaa gacgagtatg agtcaattat cccagaggaa ttaaaatggc gaaattgggc
    20341 tcatgctcaa aatggggaac gggtattgac aggcgatgaa ttacttgatt ttgtcaataa
    20401 caagttattc aaagagttga aagagcttga aataacttca attatgccta ttcgaaaaac
    20461 gattgttaaa tcagcttttg aagatgcgaa caactatatg aaaaatggcg tcttgttacg
    20521 ccaagtcatc aatgttattg atgaagttga tttcaatagc cctgaagatc gtcattcgtt
    20581 taatgatatt tacgaaaaaa ttcttaaaga tattcaaaat gctgggaact caggagaatt
    20641 ttatacgcca cgtgcagcga ctgattttat tgccgaagtt cttaacccaa aacttggaga
    20701 atcaatggca gaccttgctt gcggaacagg aggcttcttg acttcgactc tgaaccgttt
    20761 aagtagtcaa cgtaaaacta gtgaagatac caaaaaatat aatacagctg tttttggtat
    20821 tgaaaagaaa gcatttcctc atcttttagc agttacaaat ctgtttcttc acgaaattga
    20881 tgaccctaaa attgttcatg gaaatacttt ggagaaaaat gttcgtgaat atacggatga
    20941 tgaaaaattt gacattatta tgatgaatcc accttttgga gggtcagaat tagaaacaat
    21001 aaaaaataac tttccagcag aattacggag ttctgaaaca gctgatttat ttatggctgt
    21061 cattatgtat cgtttgaaag aaaatggtcg tgttggagtt attttacctg atggttttct
    21121 atttggtgaa ggtgtaaaaa ctcgcttgaa acaaaaactg gtagatgagt tcaacttgca
    21181 tacgattatt aggttgcctc atagtgtctt tgcaccgtat acaggaatcc atacgaacat
    21241 tcttttcttt gataaaacaa agaaaacaga agaaacttgg ttttatcgtt tagatatgcc
    21301 agatggttat aaaaatttct cgaaaactaa gccgatgaag tcagaacact tcaatcctgt
    21361 tcgtgactgg tgggaaaatc gtgaagagat tctggaaggt aagttctaca aatctaaatc
    21421 atttacacct agtgaattgg ctgagttgaa ttataattta gaccagtgtg gttttccaaa
    21481 agaggaagag gaaatcttaa atccctttga gttgattcag aattatcaag cggaaagagc
    21541 aactttaaat cataagattg ataatgtatt agctgatatt ttgcagttgt tggaggacaa
    21601 ataatgacac cagaacaact taaagcaagt attctccaaa gagcgatgga agggaaatta
    21661 gtgccgcaaa atcccaatga cgaacctgca agtgaattat taaagagaat taaagctgaa
    21721 aaagaaaaac ttatcagtga aggaaaaatc aaacgagata aaaaggaaac tgagatattt
    21781 cgtggtgatg atgggaaaca ttatgggaag tttgctgatg gaagtactca agaaattgat
    21841 gttccttatg atattcctga tacttgggag tgggtgagga taaaatcaat ttattggaat
    21901 tttgggcaaa ataagccaga gaaatccttt aggtatatag atacgtctag tattgataga
    21961 aaaaagaaca taatcaacta caaaaatcta caatatcttt cacctgaaca agcgccttcc
    22021 cgtgctagaa aattagtttc gcagaatagt gtcttatttt caacagttag accatatcta
    22081 aaaaatattg ctgtagttag agaacttaaa gagtatttga tagctagtac agcatttatt
    22141 gttttggata ctttacttaa c
//
LOCUS       DAVKLX010000035        22047 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKLX010000035 DAVKLX010000000
VERSION     DAVKLX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22047
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.035"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(57..1076)
                     /gene="adhP"
                     /locus_tag="VW325_001457"
     CDS             complement(57..1076)
                     /gene="adhP"
                     /locus_tag="VW325_001457"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9734831.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(1415..2227)
                     /locus_tag="VW325_001458"
     CDS             complement(1415..2227)
                     /locus_tag="VW325_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9734832.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            2417..3835
                     /locus_tag="VW325_001459"
     CDS             2417..3835
                     /locus_tag="VW325_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9734833.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            3888..4595
                     /locus_tag="VW325_001460"
     CDS             3888..4595
                     /locus_tag="VW325_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9734834.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGLVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            4690..5664
                     /gene="folP"
                     /locus_tag="VW325_001461"
     CDS             4690..5664
                     /gene="folP"
                     /locus_tag="VW325_001461"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9734835.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGKFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFGQAFTEEELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            5666..6988
                     /locus_tag="VW325_001462"
     CDS             5666..6988
                     /locus_tag="VW325_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9734836.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNVTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            6969..7523
                     /gene="folE"
                     /locus_tag="VW325_001463"
     CDS             6969..7523
                     /gene="folE"
                     /locus_tag="VW325_001463"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9734837.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            7566..8378
                     /gene="folK"
                     /locus_tag="VW325_001464"
     CDS             7566..8378
                     /gene="folK"
                     /locus_tag="VW325_001464"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU9734838.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIEKMRNRGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(8423..8740)
                     /locus_tag="VW325_001465"
     CDS             complement(8423..8740)
                     /locus_tag="VW325_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9734839.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            9095..9541
                     /gene="rplM"
                     /locus_tag="VW325_001466"
     CDS             9095..9541
                     /gene="rplM"
                     /locus_tag="VW325_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9734840.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            9560..9952
                     /gene="rpsI"
                     /locus_tag="VW325_001467"
     CDS             9560..9952
                     /gene="rpsI"
                     /locus_tag="VW325_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9734841.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(10213..10635)
                     /locus_tag="VW325_001468"
     CDS             complement(10213..10635)
                     /locus_tag="VW325_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9734842.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            10782..10958
                     /locus_tag="VW325_001469"
     CDS             10782..10958
                     /locus_tag="VW325_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734843.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            11359..13572
                     /locus_tag="VW325_001470"
     CDS             11359..13572
                     /locus_tag="VW325_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9734844.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFATEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(13794..14270)
                     /locus_tag="VW325_001471"
     CDS             complement(13794..14270)
                     /locus_tag="VW325_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9734845.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQR
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINTFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            14493..17696
                     /locus_tag="VW325_001472"
     CDS             14493..17696
                     /locus_tag="VW325_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU9734846.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNREKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRISYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            18076..18461
                     /gene="tnpA"
                     /locus_tag="VW325_001473"
                     /pseudo
     CDS             18076..18461
                     /gene="tnpA"
                     /locus_tag="VW325_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(18840..19469)
                     /locus_tag="VW325_001474"
     CDS             complement(18840..19469)
                     /locus_tag="VW325_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9734847.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(19479..20480)
                     /locus_tag="VW325_001475"
     CDS             complement(19479..20480)
                     /locus_tag="VW325_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9734848.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(20511..21152)
                     /locus_tag="VW325_001476"
     CDS             complement(20511..21152)
                     /locus_tag="VW325_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9734849.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(21171..21986)
                     /locus_tag="VW325_001477"
     CDS             complement(21171..21986)
                     /locus_tag="VW325_001477"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9734850.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGEANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         6613 a         4040 c         4870 g         6524 t
ORIGIN      
        1 tttaatataa aaaagttgga agaagcttcc aacttttaaa ataggtttct attagattag
       61 tgggtgaagt cgagtaccat acgtccttgg atttggcctt tttccatttc gtcgaaaatg
      121 gcaacagcat cttctactgg acgtttttga acaactggga ctaccagacc ttctgcacca
      181 aattggaagg cttcttctaa gtctttacga gttccgacaa gagaaccgat gacttggatt
      241 ccatcgagga ctgttttaac gatgctgagt tccatcattt cagaaggaag accaacagcg
      301 acgacgcgac caccagcacg aatggagtca acagcctggt tgaaggcaac tttagacaca
      361 gcagttacga cagctgaatg agctcctcca tcagtttttt ctttaatgag tccagctaca
      421 tcttcaactt cgaggccgtt aatcacaatg tcagcgccta cttcttttgc aagggcaagt
      481 ttgtcattgt tgatatcgac tgcgataaca tgagcattga atactttttt agcgtattga
      541 acagcgaggt taccaagtcc accagcaccg taaagaacaa cccattggcc tggttcaact
      601 tttgcttctt tgatagcttt ataggttgtt actccagcac atgtgataga agaagcttgg
      661 gctggatcaa gtccgtcagg aactttgaca gcatagtcag cagttacgat acattgttca
      721 gccataccac cgtctactga gtagccagca tttttcactg tacggcaaag ggtttcgcga
      781 ccagttgtac agtattcgca agtgccacat ccttcaaaga accaagcaac gctgacgcgg
      841 tcaccgactt taaggctttt cacatctgga gcaatctctt taacgatacc gataccttcg
      901 tgcccaagaa cacgtcctgg gacttgacca aagtcaccat gagcaacgtg gaggtcggtg
      961 tggcaaacac cacagtattc aacttctaca agtgcttccc cagtttcaag tggacggagt
     1021 actttttctt caatagcaac accagtgctt tctggattta caacaacagc tttcatagct
     1081 atcctccttg atattgaccg agaacaggga caacgggact ggattgattt tttgtcaaca
     1141 gagtcgagtg tgacgagctc tcttgtattt atatgtgaag tatatcacaa aagaaagcct
     1201 tttccaaggt tttggagaca aaaagtagaa caatcgatta actggttgat gagactgtga
     1261 aaagagcaca aagaccagct tgcaagctgg tcatactctt cgaaaatctc ttcaaaccac
     1321 gtcagcttca tctacaacct caaagcagtg ctttgagcaa cctacggcta gcttcctagt
     1381 ttgctctttg attctcattg agtatcagta taggttataa gaataagtct tgtagggttt
     1441 tggcaacccc atcttggtcg ttggtaaggg aaatttgctc atctgcataa gggagtagct
     1501 ctggattggc atttttcatg gcataaccct tcccagcaaa agcgagcatt tcggtatcat
     1561 tgtgttcatc tccaaaggca atcaaatctt ttttgtcacg attcattatc ttgagcaagt
     1621 agtccaaagc aaaggccttg ttgacacctt ttggggtaca ttcaaggata ttgagcggac
     1681 ctccccaggt attgatagaa agttgatgct ggtagaaggc gttcatgtct tttgccaagg
     1741 catatttgtc actggctctg gtctgcaaga ggatacagtt agggtccttg gtcaccaatt
     1801 caggctggaa ttgatcttca ggctggaaag cttctacacc aaatagtttg ggattggcaa
     1861 tttcttcatt gggatttgta atgtagaatt ttttacgata ttctccagcg ataaaatcgg
     1921 cttgaatgtc ctctgaacgt tgaaccatat ctagcagata ttttttgtct acagtcaaac
     1981 acttttcaaa atcccaaact tggtctggta aatgagtaag ggatccgttg aagttaatca
     2041 taggagtgtc taagcccagt tcacggtaaa aatcttttga catacggtaa gggcgacctg
     2101 tcgtaataat aacctgatgg cctttttcag caactttttt gatggttctt ttggtaaagt
     2161 cagaaatttg actgtctgag ttgagcaggg ttccatccag gtcaactgca ataatttttt
     2221 tcgtcatatg gacctttcta gtgtgttttc agataagatg tcttctatta taacagaaag
     2281 caggcagaaa agcagattcc aaacaaaaaa tgaaattgga ttaatttctt aaacaaatca
     2341 aacaaagaca ttgaaaaagt gaatgataaa tgatataatt ctattattgt tcgtaaaaat
     2401 taaaaggaga ttgatgatgg acaaattatt taaactaaaa gagaacggta cagacgttcg
     2461 tacagaggtt ctcgctggtt taacaacttt ctttgcaatg agctatattc tctttgtaaa
     2521 cccacaaata ctttcacaaa caggaatgcc tgctcagggc gtcttcctag cgacgattat
     2581 tggtgcagta gcgggtacct tgatgatggc tttttatgct aacttacctt atgcccaagc
     2641 gccaggtatg ggactcaatg ccttctttac ctttacagtt gtattcgggc ttggttattc
     2701 ttggcaagaa gccctagcta tggtcttcat ctgtgggatt atttcattga ttattacctt
     2761 gacaaatgtt cgtaaaatga tcattgaatc gattcccaat gctcttcgct cagctatttc
     2821 agctggtatc ggtgtcttcc ttgcctatgt agggattaag aatgctggac ttttgaaatt
     2881 cacgattgat ccaggcaact atactgttgt aggagaaggg gctgacaaag ctcaagcaac
     2941 gattgcagca aactcttcag cagttccagg attggtcagc tttaataatc cagctgtttt
     3001 agtggctctt gcaggacttg ccattactat cttctttgtc atcaaaggga ttaaaggggg
     3061 aattattctc tctatcttga caacaactgt tcttgctatt gcagttggtt tggttgattt
     3121 gtctagtatc gattttgcta ataaccatgt tggtgcagct tttgaagatt tgaagacaat
     3181 ctttggtgca gctcttggtt cagaaggttt gggagctttg gtttcagata cagctcgctt
     3241 gcctgaaact ctgatggcca ttcttgcctt ctcattgaca gatatttttg acacaattgg
     3301 taccttgatc ggtacaggtg aaaaagttgg tattgtagcg acaaatggtg aaaatcacca
     3361 atcagccaaa ttggataagg ctctttactc tgatttgatt ggaacgacag tcggtgccat
     3421 tgcaggtact tcaaacgtaa cgacttatgt tgagtctgct gctggtatcg gtgcaggtgg
     3481 acgtactggt ttgacagcct tggttgtagc tatctgtttt gcgatttcaa gcttctttag
     3541 cccacttcta gcgatcgtac caacagcggc tacagctcca atcttgatta tcgttgggat
     3601 tatgatgctt ggtagcttga aaaatatcca ttgggatgat atgtctgaag cagttcctgc
     3661 cttcttcaca tctatcttta tgggattcag ctactctatc actcaaggga ttgcagttgg
     3721 tttcttgact tacactttga ctaagcttgt caaaggtcaa gttaaagatg ttcatgccat
     3781 gatttggatt ttggatgcct tgtttatcct taactacatc agcatggcct tataatagaa
     3841 tgacccaggg ggattttccc ccttttttaa tacaaaggag ataggtgatg aaagagaaaa
     3901 atatgtggaa agaattgttg aatcgtgcag gctggatttt ggtcttttta cttgccgtcc
     3961 ttttatatca ggttccccta gtggttacct ctattttgac tttaaaagaa gtagccctgc
     4021 tacagtcagg gctgatagtt gctggccttt caattgtggt tctggctcta tttattatgg
     4081 gagctcgtaa aaccaagtta gctagtttta atttttcttt ttttagagct aaagatttgg
     4141 cacgtttggg cttgagttat ttagttattg tcgggtcaaa tatacttggt tctatcttgt
     4201 tgcaactgtc aaatgagacg acaacagcta accagtctca gattaacgat atggttcaga
     4261 atagttctct gatttctagc ttcttcttgc tagccttgct tgctccaatt tgtgaggaaa
     4321 tcttgtgccg tgggcttgtt cctaaaaaga ttttccgagg caaggagaac ttgggatttg
     4381 tagtcggtac gattgtgttt gctttattgc atcaaccaag taatttacct tctttattga
     4441 tttatggagg tatgtcgaca gttctatctt ggacggccta caagacccaa cgtttggaaa
     4501 tgtcgatctt gcttcacatg attgttaatg ggattgcttt ctgtttgttg gctcttgtgg
     4561 tgattatgag tcggacatta ggaatttctg tgtaaaagtt tttatgtagg aaccgacctc
     4621 tttctactag ggaaagatga atgcaatcgt gtccatcttt ttctttttat ggtaaaatag
     4681 aaaaataata tgatgaaaat ccttgaggga gtgaccgata tgtcaagtaa agccaatcat
     4741 gcaaagacag ttatttgcgg aattatcaat gtaaccccag actccttttc ggacggtggt
     4801 aaattttttg ctcttgagca ggcactccag caggctcgta aattgatagc agaaggagcc
     4861 agtatgctcg atatcggcgg agaatcgact cggccgggca gtagctatgt tgagatagaa
     4921 gaggaaatcc agcgtgttgt tccagtgatc aaagcgattc gcaaggaaag tgatgtcctc
     4981 atctctattg atacttggaa gagtcaagta gcagaggctg ctttggctgc tggtgccgat
     5041 ctagtcaatg atatcactgg tcttatgggt gatgagaaaa tggctcatgt ggtagctgaa
     5101 gcgagagcgc aagtggtcat catgtttaat ccagttatgg cgcgacctca gcaccctagc
     5161 tcgctcatct tccctcattt tggttttggt caagctttta cagaggaaga gttagctgac
     5221 tttgaaacat tgccaatcga agaattgatg gaggctttct ttgaacgagc actagcgaga
     5281 gcggcagaag ctggtattgc accagaaaat atcctgttgg atccaggaat tggctttggt
     5341 ctgaccaaga aagaaaatct gcttctttta cgggacctgg ataaactaca tcagaagggc
     5401 tatccaatct ttctcggagt gtcgcgcaag cgatttgtca tcaatatcct agaggagaat
     5461 ggttttgaag tcaatcctga gacagagctt ggtttccgaa atcgggacac ggcttcggct
     5521 catgtaacta gtatcgctgc gagacagggt gtagaagtgg tgcgcgtgca tgacgtagct
     5581 agtcacagga tggcagttga aattgcctct gccattcgtc tggctgatga agcggaaaat
     5641 ttagatttaa aacaatataa ataagatgaa agaaattgaa aacaatcagt ggattgctaa
     5701 ctaccggacg gatcaaccgc attttggatt ggaacgaatg gtggaactgt tagctttgcg
     5761 tggcaatccc catctcaaac tcaaggtcct ccatatcgga gggactaacg gcaagggctc
     5821 gactattgct tttttgaaaa agatgctaga aaagctaggg ctgagagttg gcgtgtttag
     5881 ctcgccctat ctcattcatt acacagacca gattagcatc aatggggaat cgatctcaga
     5941 agcgaggcta gaagctctca tggcagacta tcagtctttg ctggagggag aagcggtcgc
     6001 caatttacag ggcacaaccg agtttgagat tatcacagcc ctggcctatg actactttgc
     6061 ctcagagcaa gtagatgtgg ccatcatgga agttggcatg ggtggacttt tggatagtac
     6121 caatgtctgt cagcccattt tgacaggaat tacaactatt ggcttggatc atgtggccct
     6181 acttggtgac accttggagg ccatagcaga gcagaaggca ggtattatca aacaagggat
     6241 gcccttggta acagggcgta ttgctccaga agccttgact gtgattgacc gcattgcgga
     6301 agggaaagat gcgccgagac ttgcctacgg gacagattat caggttcgtc atcaagaaag
     6361 tgtggtgaca ggggaagtct ttgactatac aagtgctgtc agacaaggtc gcttccagac
     6421 tagcctgctt ggtttgtacc aaatagagaa tgctgggatg gccatagctt tacttgatac
     6481 tttttgtcaa gaagatggtc gagagctagc aagcaatgac tttcttggtc aagccttgga
     6541 agaaacaagt tggccagggc gtttggaaat cgtgtcaaga gatcccttga tgattttgga
     6601 tggagcccat aatccccatg ctatcaaggc cttgttggta accttgcaag aacgttttgc
     6661 ggattatcat aaggaaatcc tcttcacttg tatcaaaacc aaagccttgg aggatatgtt
     6721 ggacttgctg ggagccatgc cagataccga gcttactcta acacattttg cggatagtcg
     6781 ggcgacggat gaaagcgtgc tgaaagaggc agctaagtct agaaatctca gctaccaaga
     6841 ttggcatgat tttctagagc agaatgtgac agataaaaaa gaagagaaac aaacagttag
     6901 gattgtcaca ggttccttgt atttcttgag ccaagtgagg gcctatctga tggagaggaa
     6961 gaacgagaat ggatacacaa aagattgaag cagctgtaaa aatgattatc gaggctgtag
     7021 gagagaacgc taatcgcgag ggcttgcagg aaacacctgc tcgtgtagcc cgtatgtatc
     7081 aagagatttt ttcaggtctt ggtcaaacag cagaggaaca tttgtcaaaa tcctttgaaa
     7141 ttattgacga taatatggtg gtagaaaagg atatcttttt ccataccatg tgtgaacacc
     7201 acttcttgcc attttatggt agagcgcaca ttgcctacat tccagatggt cgtgtggcag
     7261 gcttgtctaa gctagcccgt acggttgaag tttattcgaa aaaaccacaa attcaagaac
     7321 gtttgaatat cgaagtggcc gatgccttga tggactatct aggtgctaaa ggagcctttg
     7381 ttgtcattga ggcggaacat atgtgtatga gtatgcgtgg tgttagaaaa ccaggcactg
     7441 caaccttgac gacagtagct cgtggtctat ttgaaacaga taaggacctc cgcgaccaag
     7501 cttatcgttt aatggggcta taaaaagaat ccgcttcaag cggatttttc tagaaaggaa
     7561 tcattatgga tcaactgcag attaaggatt tggaaatgtt tgcctatcat ggtctttttc
     7621 ctagtgagaa agaattgggg cagaaatttg tcgtttcagc catcctatcc tatgatatga
     7681 ccaaggcagc tacagacttg gatttaacag cctctgtcca ttacggagaa ttgtgtcagc
     7741 agtggacgac ttggtttcag gaaacgagtg aagatttgat tgaaacggta gcctataaac
     7801 tggtggaacg tacctttgag tcttatcctc ttgtccaaga aatgaagttg gaactgaaaa
     7861 aaccttgggc accggtgcat ttgtcactag atacttgctc ggtaaccatc catcgccgca
     7921 agcaacgagc ctttatcgcc ctaggaagca atatgggaga taaacaagca aacttgaaac
     7981 aagccattga gaaaatgcga aatcgaggta tccatattct caaagagtcc agtgtcttag
     8041 cgacggagcc ttggggtgga gtggagcagg atagctttgc caatcaagtg gttgaggtgg
     8101 aaacctggct accagcacaa gacttgttag aaaccttgtt agccattgag tcagagctgg
     8161 gacgggtgag agaagtgcat tggggacctc gtttgattga tttggacttg ctctttgtgg
     8221 aggaccagat cctttataca gacgacctca tattgcctca tccttacata gcggaacgcc
     8281 tttttgtcct tgagtcctta caggaaattg cgcctcattt tatccatccg atattaaaac
     8341 aaccgatccg caacttgtat gatgctttga aaaaatagaa aaactctagt tttcagttac
     8401 ttgcaactga aggctagagt ttttaaaata ggtcattttc ttctgggagg aggatagttt
     8461 ctctaccgtc catgtctaaa accagtactc ttgggggata acgagggtcg aaaggatggt
     8521 taaagtcaaa atcaatggct gtagggaggt gttgacttga aaagtggaag gtaatctttc
     8581 cttggttatt aagcaattga aactcgagtt cttcttccaa ttcaaagaca ttttttaaga
     8641 aatgatcgat gatataccaa aaagagtcaa tgatgtcatc gggcaagctg gtaacaatgc
     8701 caaaactagc agaccgcatg tgggtattgg taaaagccat atctctgtcc cctttctttt
     8761 cccttatcat acagcaaata ggattaaaaa tcaagaaaag gtgatttttt gaaaaggatt
     8821 ttagttacag ggagaaatag ggaaaaattc ccaaaaatct accgaagtta ataggtaaat
     8881 tcccaaatta acttgattat ataactttca gttattttga gaagttaccg aaaaatattt
     8941 tttatatcta ttgactttta ggggtgaaat ttggtatgat agtaggcggt attgtttacc
     9001 ccatttgaaa ggccccggaa ccttccaaat acttttcgat gggaaggaac acccatcacc
     9061 gtaaacaaaa atcgaactat atataggaga aatcatgaac aaaacaacat ttatggctaa
     9121 accaggccaa gttgaacgta aatggtacgt agttgacgca actgatgtac cacttggacg
     9181 tctttctgca gtagttgcta gcgtacttcg cggaaaaaac aaaccaacat ttacaccaca
     9241 cactgataca ggtgacttcg tgattgttat caatgctgaa aaagttaaat tgactggtaa
     9301 aaaagcaact gataaaatct actacactca ctcaaaccac ccaggtggat tgaaacaaat
     9361 ctctgcaggt gaacttcgtt ctaaaaatgc agtacgtttg attgagaaat cagttaaagg
     9421 tatgcttcca cacaatacac ttggacgcgc tcaaggtatg aagttgaaag tatttgttgg
     9481 agctgagcac actcacgctg cacaacaacc agaagttctt gacatttcag gacttatcta
     9541 aggaaaggaa caataaagta tgtcacaagc acaatatgca ggtactggac gtcgtaaaaa
     9601 cgctgttgca cgcgttcgcc ttgttccagg aactggtaaa atcactgtta acaaaaaaga
     9661 tgttgaagag tacatcccac acgctgacct tcgtcttgtc atcaaccaac cattcgcagt
     9721 tacttcaact gtaggttcat acgacgtttt cgttaacgtt ataggtggtg gatacgctgg
     9781 tcaatcagga gctatccgtc acggtatcgc tcgtgccctt cttcaagtag acccagactt
     9841 ccgcgattca ttgaaacgcg caggacttct tacacgtgac tcacgtaaag ttgaacgtaa
     9901 gaaaccaggt cttaagaaag ctcgtaaagc atcacaattt agtaaacgtt aattcgaaag
     9961 aattactata cttatacaga gcacctttcg gggtgttctt ttttatactt tcttactaaa
    10021 ttggtgcaat tgacacagtt gttgcgactt tagtcgctta caaatgtggc tgcaacctga
    10081 caaggtcagt tgcctcaaaa cgttaatcaa tacgattata tcaacgtttc aaagcactca
    10141 agagtttacc tcatgggtgc tttttttatg ttttttgaaa aagttcacct caaagtttac
    10201 cttattttca ccttattgtt ttagagactg taaggcaaat tcaccgacgg ccatagggac
    10261 tacattagaa gtattgacat aaatctgtgt tgtacctgtg tcgctatgta ttagagcttc
    10321 agaaatggct tctaagctca ttcctgcttg tttagcgagc gttgctccgg tatgacgtag
    10381 tttgtgtggc gtagcgtgtg taagctctgg gtgtcgtttt ctgatagttt tcatcttatt
    10441 attgagatag tcagaatgta aaggtttatt gatattgcct ttggtatcta tgtaagtaaa
    10501 gatgaattgt tctggattac tgatgatacc aaactttgcc agttcatatt tttgttgttt
    10561 tttccatgaa gtcagaagtt gaagtaagtc attagaaata gagaaaatgg gaaatgtttc
    10621 cacaataaaa cgcatattat cagatggtta atgcgtttta tatttgggtt ttaggcttat
    10681 aaaataggat aagcgtactg aaaacttaga tatgacaaac aaaataaaat ttttaaaagc
    10741 tacagagaaa catggtataa tgtatctaaa ggaggtagca tatgtcctta gatatagata
    10801 aagaaaagat gacaatcatg ggaatagctt ttgaaaaccg atccgttttt aagagtgttt
    10861 ggtatgcttt aagtacaaat atgattgaag gatggcgacc tacggttagt gatgttgaaa
    10921 aattacgaga tgaagctctt gctctgggga tgacttaatg aagcgcaact gttatgagtc
    10981 ttatgactat gttgatccgg ataatttata taccgatccg ggttcttcag ttctaagaaa
    11041 taagcaggaa ggaaaaaagc acgcgagctt gaatatcgta tgattgctag taagagtttg
    11101 aaattattta tcaatcctat cttagttaat agtttaaaag tagtttgttt cacaactgca
    11161 taagcttaga aaagttgaaa agtgataact ctttaataaa ctcttggttt gttaaaagat
    11221 attttgtcca ctacattttt tcaattgact attatttgtc atagatataa cagatagaac
    11281 attgcattca gaataatgaa aaacgcatca aaaatggtgc gttctgtttt tatgctacca
    11341 taaaaggagg aaaacaaatt gaaaattttt aagggagagt tttatcgaat ctctgtatta
    11401 acagacaagc tagtaaggtt agaatactct caaactggaa gttttgagga tagaacgaca
    11461 caacttatct ataatagaga ttttggccaa gtttcgttag attatatcga gacatcaaac
    11521 gtactagata ttatgacgga ctattttcat ctgcacttta ataaaggaga atttaacgcc
    11581 gaaaatttat ttatagaatt aaaaggaaat tttgccgtat atggtagtcg ctggtatttt
    11641 ggtgaatcta ttgaaacgtt aaaaggaaca gctcggactc tggataaggc agatggagca
    11701 atctcgttag aagatggaat tattagccga aatggtatag ccttattgga tgattctcaa
    11761 ggatttattt gggatgaaca atctggttat attgagagag aaaatcaaat tgacctgtat
    11821 ttctttgcct atgggcatga ttatagagga gcaatcagag acttttacca tttgactggt
    11881 tcaacaccct tgttgccaag atatgcttta ggcaattggt ggagtagata ttggccttat
    11941 acgtcggatg aatacttgga tttaatagac agatttgcaa cagagaaaat tccattatct
    12001 atcggtgtgt tagatatgga ttggcatata actgacattc cagctcgttt tggaagtggc
    12061 tggacaggat atagttggaa tagaaactta ataccaaatc cagaacagtt attgcaacaa
    12121 cttcatgata gaaagttaaa actctcctta aatgtccatc ctgctgatgg gatacgggct
    12181 tatgaagaag cttatcctca aatcgcaaaa cggttggggt taaatgtaga actagaagaa
    12241 cctgctattt ttgatttttt taatccctct tttagggaag cctactttaa agatgttcat
    12301 tatgagctag aaaagcaggg agtagatttt tggtggattg actggcaaca ggggacacaa
    12361 ggcatgctag atccactttg gcttttaaac cattatcact atcaggatag ttgtaaaaat
    12421 gcagaaggtg gtttgatttt atcaagatat gcaggtcctg gtagtcaccg ctaccctgtt
    12481 ggtttttcag gggatactat tatcagttgg aattccttaa gatttcaacc ctattttaca
    12541 gcgacagcat ctaatatcgg ttatagttgg tggagtcatg atatcggtgg acatatgttg
    12601 ggggattatg acgaagagct acaaactaga tggctacagt ttggtgtttt tagtccgatt
    12661 actcgattac atagttctag aagtcctttt aatagtaaag aaccttggtt tttttcagaa
    12721 acaacatcta agattatgaa gaaatacctt cgtttgagac atcagatgat tccttatcta
    12781 tataccatga atgtaaagac acatgaggaa ggtgccccat taatcagtcc aatctattat
    12841 ttctatccag agaatgatga gagctataat gttccaaacc aatacttttt tggaacagaa
    12901 ctgatggtgg ctcccattgt agaaaagatg gatttgacat tccaatctgc aaaagtagat
    12961 gtatggttcc ctgaaggtga atggtatgac ttcttttcag agaaaaaata cacaggtggt
    13021 gtgaagttaa gtgtttatag ggacatctcg actacgcctg tgtttgcaaa aagtggtgca
    13081 atcattccct tggttggttc tgagataggt atgggtgttg atttacctga ggttgtagat
    13141 tggtatgtat tcccagggaa acaacattct tttgaaatgc ttgaagatca aaatggtcaa
    13201 agatataaaa caagattatc aatcgattgg gaaatgggaa tggtagagtt agcattacaa
    13261 ggagattcta gtatcgttcc aagcaataga aaacatagaa ttcattttaa aggaacgaat
    13321 gtgtctataa ttgaattgcc aaataagaat gatacagcta aatttgaatg gaaagataat
    13381 aaaaggacat ctttaaatga cgaagttttt agactactaa agacagcttc tcttccatat
    13441 gaattaaaag atagattgtt aaatcaattc atcaatgcga aaaactctca tgacttaatg
    13501 aatatcttgc atcatcagga taaggaattg agagggcgtt tgttggaaat gatatttact
    13561 agccaaaact aattgaaata attagaaagt catttgttta gtgtatatga agattctgta
    13621 tggtattcgt tatcactggt cacattgaaa atgtgaaata agcacctaca acatagactt
    13681 tacaacactt catgtttcat atcatggggt gtttttctat ttaagtcaaa gccaatcaaa
    13741 aaatctcact attcatcagg ttaaaggaaa cctaatcata gtgagattgt gaattatagt
    13801 agagtttgta tcgcctcttc aatttgtttt gggtctgttt ttgaagaaaa gcgttcaaag
    13861 acttgcccat cacgaccaat gagaaactta gcgaaattcc attcgattcg ttttcctagt
    13921 gggccagatt tatggtcttt taaccagaca tagagagggt ctgcttcctt accgttgacc
    13981 ttaatcttgg caaaacgtgg gaaggtggtt tgaaaatgta ggctacagaa ggtgttgatt
    14041 tcctctgcgc tgccgggtgc ttgtcccata aactgattgc aagggaaatc caatatttcg
    14101 aagccctgat cttgatagcg ttcatagagt tcttgaagtc tctggtactg gggcgttaaa
    14161 ccacatccag tagcagtgtt gacaatcaag agaactttgc cacgatagct atccaaggga
    14221 gttgcttggt tgttttggtt caaaacggaa aaatcataaa gtgaagtcat gagggcctct
    14281 tttctttttc tacattgtaa cagtttttac ttttttcgtc gagtcggatg gaagatattg
    14341 ttgaagaata agccctcaat atgatagaaa ccgtttacaa ataactttaa aaatggtatg
    14401 atttaattaa accggtttac tttgttgcta taaatctcta atttgagatt tagttcctat
    14461 tgattttaca atatgtttat tggagtgtat acatgcaaac aaaaacaaag aagctcattg
    14521 tgagtttgtc ttcacttgtt ttatcaggat ttttattaaa ccattatatg acagttggag
    14581 cggaagaaac gactacgaat accattcagc aaagccagaa ggaagttcag tatcagcaaa
    14641 gggatacaaa aaatttagtt gaaaatggtg attttggtca gacggaggac ggaagcagtc
    14701 cgtggacagg aagcaaagct caggggtggt cagcttgggt agaccagaag aatagttctg
    14761 cagatgcctc aactcgagtc attgaggcta aggatggggc tatcactatc tcaagccctg
    14821 agaaattaag ggcagcggtt caccgtatgg ttcctattga agctaagaaa aagtataaac
    14881 tgcgtttcaa gattaaaaca gataataaag tcgggattgc caaggttcgt atcattgagg
    14941 aaagtggtaa ggacaagcga ttgtggaatt ctgcaacgac gtcaggaaca aaggactggc
    15001 agaccattga agcagactat agcccgactt tagatgttga taaaatcaag ctggagttat
    15061 tctatgaaac aggaactggg actgtttcct ttaaggatat tgagctggta gaggtagcag
    15121 cccagctttc tgaggattct caaacagata aacagcttga ggaaaagatt gatttaccaa
    15181 ttggaaaaaa acatgttttt tctcttgcgg actatactta taaggtagaa aatcctgacg
    15241 ttgcttcagt caaaaatgga attttagaac ctcttaagga agggacaacc aatgtcattg
    15301 tcagtaaaga tggcaaggaa gtgaaaaaga ttcctttgaa gattctagcc tctgttaagg
    15361 atgcatacac agcccgtttg gatgactgga atggcatcat cgctgggaat caatactatg
    15421 attctaaaaa tgaacagatg gccaaattaa accaggaatt ggaaggaaag gtagctgata
    15481 gcctatctag tatttcaagt caggcggacc gcacctattt gtgggaaaaa ttttcaaatt
    15541 ataaaatgtc tgcaaatctg actgccactt atcggaaatt ggaggagatg gccaagcaag
    15601 tgaccaatcc ttcttctcgt tattatcaag atgaaactgt cgttcgaaca gtcagggatt
    15661 ccatggaatg gatgcataaa catgtctaca atcgtgaaaa gagcattgtt gggaactggt
    15721 gggattatga aatcggtaca cctcgtgcca tcaacaatac cttgtctctg atgaaagaat
    15781 acttctctga tgaggaaatt aaaaaatata cagatgtgat tgaaaaattt gtaccagatc
    15841 ccgaacattt ccgaaagacg actgataacc catttaaggc tctaggtgga aacttagttg
    15901 atatgggaag agtaaaagta atagctggtt tactgcgtaa ggatgatcaa gaaatttctt
    15961 ctaccattcg ctcgattgag caagtgttca agttggtaga ccaaggtgaa ggtttttatc
    16021 aagatggatc ctatatcgac cacaccaatg ttgcctatac gggtgcttat gggaatgttt
    16081 tgattgatgg cctgtctcaa ctgttgccag tcattcaaaa gaccaagaat ccaatcgata
    16141 aagataaaat gcaaaccatg taccactgga ttgataaatc gtttgctcct ttgctggtga
    16201 atggagagct gatggatatg agtcgtggac gctcgatcag tcgtgcaaat agcgaggggc
    16261 acgtggccgc agtagaagta ctaagaggga ttcaccgaat agcggatatg tctgaaggag
    16321 aaaccaaaca acgtttgcag agtcttgtga agaccattgt tcaatcggat agttattatg
    16381 atgtctttaa gaatttgaag acttataagg atatcagttt gatgcaatcc ttgttaagtg
    16441 atgcaggagt cgcaagtgtt ccaagaataa gttacctatc tgcctttaac aagatggata
    16501 aaacagccat gtacaatgca gagaaagggt ttggatttgg cttgtcactc ttttccagtc
    16561 gtaccttgaa ttacgaacac atgaacaagg aaaataaacg tggttggtat acgagtgatg
    16621 ggatgttcta tctttacaat ggcgatttga gtcactatag cgatggctac tggccaacag
    16681 ttaatccata taagatgcct ggtacaacag agacggatgc taagagagcg gatagcgata
    16741 caggtaaagt tttaccgtct gctttcgttg gaacgagcaa actagatgat gccaatgcga
    16801 cagcaaccat ggatttcacc aactggaatc aaacattgac tgctcataag agctggttta
    16861 tgctaaagga taagattgcc tttttaggaa gcaatatcca aaacacttca acagatactg
    16921 ctgcaactac aatcgaccag agaaaactgg aatcaagtaa tccatataaa gtctatgtca
    16981 atgataaaga agcctccctt acagaacaag aaaaggatta tcctgaaacc caaagtgtct
    17041 ttttagaatc gtccgattcg aaaaagaata ttggttactt tttctttaag aagagttcaa
    17101 tcagcatgag taaggctttg caaaagggag cctggaagga tatcaatgaa ggacagtcag
    17161 acaaggaagt tgaaaatgaa tttcttacga ttagtcaggc tcataagcaa aatggagatt
    17221 cttatggcta tatgctcatt cctaacgtgg atcgtgccac cttcaatcaa atgataaaag
    17281 agttagaaag tagcctcatc gaaaataacg aaacccttca gtctgtttat gatgccaaac
    17341 aaggagtttg gggcattgtg aaatatgatg attctgtctc tactatttcc aaccaattcc
    17401 aagttttgaa acgtggagtc tataccattc gaaaagaagg ggatgaatat aagattgcct
    17461 actataatcc tgaaacccag gaatcagctc cagatcagga agtctttaaa aagctagagc
    17521 aagcagctca gccacaagta cagaattcaa aagaaaagga aaaatctgaa gaggaaaaga
    17581 accattcgga tcaaaagaat ctccctcaga caggagaagg tcagtcaatc ttggcaagtc
    17641 tagggttctt gctacttggg gcgttttatc tattccgtag aggaaagaac aactaatttg
    17701 ttcatcatct agatgaatta catgaaactg tggaaaatag aaaaaatccg aagaaggcca
    17761 aatatcttcg gatttttctt ttaaaaaagg ataagttgat acttatcctt tttacttcat
    17821 tatgtgacta tagcgttttg aaactagaat aggacatcat aactgctaaa agatttctat
    17881 aaattcattt gattttccta atcaatttgt tcgtatccta tttcactcca ctatattagt
    17941 ccgtctcgct ccgtgaggtg cgagacaaaa caaccacccg ctatacgggt gcgcgtcgaa
    18001 ggttatacca aaaaaactcc aaacgcgata caataaaggt gttcaatcca cttgtaaagc
    18061 gaaaggagaa aaaatatggc acaaaaggct catagtttat ctcacacaaa gtggaggtgt
    18121 tctatcacat tgtgttcacc cctaagtata gacgaaaagt catctataat caatatagaa
    18181 gtagtttagg cgaaatattt catcgcttgt gtagttataa aggtgttgaa atgatcgagg
    18241 gtcacttaat gccagaccat gtacacatgt tagtcagtat tccgtcgagg ctaagtgttt
    18301 caagttttat ggggtattta aaaggcaaaa gtgcactcat gatgtttgac aaacacgcca
    18361 atctcaagta caagtttggg aatcggcatt tctgggcgga aggttattat gtaagtccag
    18421 tagggcttaa tgaagccaca attaagaaat atagtcaaga ttaaagaaat tatccagtgg
    18481 atgatttctt cacgagtatg aaaatttgag aacgagtaaa gtatgatata tagtaaaatg
    18541 aaataagaac aggacaaaat cgatcaggac agtcaaatcg atttttaaca atgttttaga
    18601 agtataggtg tactatttca gtttcaatct actatagcac tagataaatt gagtgtaaaa
    18661 gaatatgagg atccctttag ggatagtggt aagtaatacc aaagtctctt aaagaggcaa
    18721 gtgacgagtc aagagcaata aggcttgaac aacgtgaaag ccagcgtctt taggcgctgg
    18781 ctgatgattt gggcttatag ctctgagata aaccacccgt tagacgggta gttatgattt
    18841 tatctgagtg taatatactg ttgggcaatc tcgctgatgc ggtcaaagtt gccttgggaa
    18901 gcgagtttat tgagttcgcc accaattcca acggcatctg caccagcagc gaaccattga
    18961 gggatgttgt ttaggccgac tcctccggtt accattacgg aaacttgtgg gatcggtgcc
    19021 ttgactgcag agatatatgc tggactgaga gtactacctg ggaagagttt gatgatttca
    19081 ctaccggctt caagtgcagt cgtgatctct gtgagggtaa tacagcctgg aatgtacggt
    19141 gtgctgtaga gattgcacat tttcgcagtt tcagcatgga aagatggaga aacaacgtaa
    19201 tttgctccag ctagaatggc atctctagca gttacggcat caagcacagt acctgcaccg
    19261 atacaaacac tctgatcgtc ctgatacaag tctacaagtt ccttgatgat ttgtcctgca
    19321 tactgattgg tataggcgat ttcaatagct ttgataccgc ccttgataca agcaatcgag
    19381 gcttgtagtc cttcttcctt tgtatttcct cgaatgacag cgacaatttt ctgttttttt
    19441 agttcaataa tcgtatctga tttggtcatg taattctcct aacgaatgat atcttgtgca
    19501 tttgccagta aattttcaat actagttgcg gaagtggaga gatggtctcc tggaagagtg
    19561 catttgagag ttgcgctcgc aactgcaaag tcaatggtag tttttaggga ggaatgatgg
    19621 agtagttggt aaagggcacc agatataaag gcatccccgc taccaattcg ttgatagact
    19681 gcagttttta gttggacaga ctcttcaaat cgttcttcca gagcataggc ttgatagaca
    19741 tttttgtctt gctcatcact agatcggagg gtatggaaaa tagccttgaa accataggct
    19801 tcttttaaaa gtcgcatgcg attttccacc tcttctaggc tagcactgtc tcttgggaac
    19861 atctctagat tttggtcatc aatcatgaga ggatcaatac cgaagcaata gtcagtaaaa
    19921 cgtgcaaact tagaaaattc atacttggct tctaggactg aaatcatctt tgttctcaga
    19981 ttgagatcca ttgaaacgac aattcctcgg cgcttggctt cttccaagag taggagaagg
    20041 atcgcacgga cctcttgacc gatagctatg gtgattccac taaaatgaaa atggctaatc
    20101 ccctgaaaga gagaatccat atctagcatg tttggccgaa tctggctgat actcgtatgc
    20161 ttacgatcgt agaaaacttc actttgacga caaccaaagc cgttctccaa atagtagagg
    20221 ccgattcgat cgccaagccg acagattgaa ctggtatcga tttggtgctg tttcaagaat
    20281 gtgagaaaac gatctccaat ctcgttggca gggagtgcgg taaagacttt cgttgagata
    20341 cccagggctt gcaaattaca agcgatgtta atttctgatc cgccaaaata agtcgaactg
    20401 gcaacatgat cgccgatact ggtggcatct aatggtgaga ttcgaattaa tggttcgcca
    20461 aacagtaata ttttcgtcat ccattagttc ctttctggtt tagaatagct ttacttggct
    20521 aatacttctt tcaaataagc agcgacttca tcatcttggc agttttcaaa gaagtattct
    20581 tggaagtatt tgccagagat ggtgtctttg aggaagtctt ggtcgattgt tttaaggatg
    20641 gtcatcaaat cattgtgggt gatttgtttc acctcgttta agatacgagc gttgcgttgt
    20701 tcagggatta cacgttctct tgggtagcca ccgcccattt cttcagcaaa taagcgttca
    20761 aacatcaatt tgagggtcag ttctgccccc cagccaaatc ctttggcata agggatagac
    20821 aaggcgttac caccattgat ttgagaataa aggtaagcgt cagttgggtc cactgctaga
    20881 ccacagacaa caccagggaa gctgtttaaa gcaagcatag cccctacacc cgtaccacag
    20941 ccggtaacaa caaagtcaac tgcctttgta tttaaaagga tggcagccat tagtccgttc
    21001 tgcacataag ttaattgact ttctccttct tctccacgca taccatagtt aaataattgg
    21061 tagccttttt tatctgtcgc ttcttttaga ctatcgtaaa taatgtgatt cttgctagct
    21121 tgactatttt cattgattaa tgcgattttc ataagatctt ctttctattt ttactcaggt
    21181 tgttttccga tgtaggctaa gataccgcca tctacatata ggatgtggcc attgacaaaa
    21241 ttgctggcat cactagcgag aaagacagca gggcccatca aatcttcagg atttccccaa
    21301 cgtgcagcag gtgtttttgc aatgatgaac tggtcaaatg ggtggcgaga accatcttct
    21361 tgcaactcac gaagaggtgc tgtttgagga gtggcaatat aacccggtcc aattccgtta
    21421 cattggatat tggcttcacc gtattcagac gcaatgttgc gggtcaacat tttcaagccc
    21481 cctttagcag cagcataagc gctaactgtt tcacgtccca gttcgctcat catcgaacaa
    21541 atattgataa tctttccatg ccctttcttt atcatagaag gaataactgc ctttgaaacg
    21601 ataaatggtg cgtttaagtc aatatcgatg accttacgga aatcagcggc gctcatttcg
    21661 cacattggaa ctcggcggat aataccagcg ttattaacga ggatgtcaat gacaccaacc
    21721 tcttgttcga tttgcttgac catggcttgg ataccgtcct cgtctgtcac gtcacagaca
    21781 tatccatggg cttcgatgcc aacttcacga taagcttcaa tccctttatt gaccagatct
    21841 tgattgatat cgttaaagac aatagtagcg ccggcttcag cgtaggattt ggcaatagca
    21901 aatccaattc cataagaagc gccggtgatg agagcaattt ttccttttaa agaaaactgc
    21961 tcaattgaga atgatgtatt tgtcatttga tacttccttt caaatttgtt aagacttctc
    22021 tataaccaaa ttatattcta gtaaacc
//
LOCUS       DAVKLX010000036        21778 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKLX010000036 DAVKLX010000000
VERSION     DAVKLX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.036"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            129..1622
                     /locus_tag="VW325_001478"
     CDS             129..1622
                     /locus_tag="VW325_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9734851.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..2528
                     /locus_tag="VW325_001479"
     CDS             1635..2528
                     /locus_tag="VW325_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9734852.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLN
                     TKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHL
                     RINSGMVILRPKTPNLNLLVR"
     gene            2602..5952
                     /locus_tag="VW325_001480"
     CDS             2602..5952
                     /locus_tag="VW325_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9734853.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQVLLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(6458..6928)
                     /locus_tag="VW325_001481"
     CDS             complement(6458..6928)
                     /locus_tag="VW325_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9734854.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(6945..9218)
                     /locus_tag="VW325_001482"
     CDS             complement(6945..9218)
                     /locus_tag="VW325_001482"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9734855.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFAYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            9565..12666
                     /locus_tag="VW325_001483"
     CDS             9565..12666
                     /locus_tag="VW325_001483"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9734856.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLIGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            12749..13756
                     /gene="pfkA"
                     /locus_tag="VW325_001484"
     CDS             12749..13756
                     /gene="pfkA"
                     /locus_tag="VW325_001484"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9734857.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            13815..15320
                     /gene="pyk"
                     /locus_tag="VW325_001485"
     CDS             13815..15320
                     /gene="pyk"
                     /locus_tag="VW325_001485"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9734858.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(16055..16280)
                     /locus_tag="VW325_001486"
                     /pseudo
     CDS             complement(16055..16280)
                     /locus_tag="VW325_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            17128..18000
                     /locus_tag="VW325_001487"
     CDS             17128..18000
                     /locus_tag="VW325_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9734859.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(18146..18490)
                     /locus_tag="VW325_001488"
                     /pseudo
     CDS             complement(18146..18490)
                     /locus_tag="VW325_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            18633..18944
                     /locus_tag="VW325_001489"
     CDS             18633..18944
                     /locus_tag="VW325_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734860.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            18948..19664
                     /locus_tag="VW325_001490"
     CDS             18948..19664
                     /locus_tag="VW325_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9734861.1"
                     /translation="MKKYFIGGLGNNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYLTSEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVLDLALLISKEKSGAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(19794..20609)
                     /locus_tag="VW325_001491"
     CDS             complement(19794..20609)
                     /locus_tag="VW325_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9734862.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(20606..21511)
                     /locus_tag="VW325_001492"
     CDS             complement(20606..21511)
                     /locus_tag="VW325_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9734863.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
BASE COUNT         6907 a         3674 c         4595 g         6602 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcca
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggca
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg
     1741 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
     1801 cagatgatat tctgatagca tgggatggag ctaatgcagg aacagttggt tatggattat
     1861 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
     1921 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagaacatt
     1981 caacaggtgc aacaattcct catttaaaca agaatatatt acttgattta caattagaat
     2041 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaagggctta
     2101 ttactaaaag aaaattgcag ttagatgaac taaacttgct cgtcaaatcc cgatttaacg
     2161 agatgtttgg ggaaaataaa atatttgaaa gcattgataa cttatttgat attatagatg
     2221 gtgatagggg caaaaattat cctaaatcag atgagttgtt tagtgaggag tactgtttat
     2281 ttttaaatac aaagaatgtt actaaaaacg gattttcatt cgatacaaag caatttatca
     2341 ctaaaacaaa ggataaatta cttcgaaaag gcaaacttga gcgttatgat atagtcttga
     2401 caacaagagg tactgttgga aatgtagcgt actacgatga attaataaaa tataaacatt
     2461 tacgtataaa ttcaggtatg gtaatattac gtcccaagac accaaatcta aatttgcttg
     2521 tgagatagct ataaaagtgt ggagaaatag cttgaaattt agtataatat agctaaacta
     2581 tttgtttaaa gtgagaaaaa aatgggaaat tttagctttc ttttaaaaaa tgacgaatat
     2641 gaatcttttt caaaaccttg cattgaagct gagaatatga ttgctacatc aactgtggct
     2701 actgccttta tggcgcgtcg tgctttagag caggctgtcc attggatata tagtcacgat
     2761 tcatatttag aagctcccta tcgtgctact ctatcttctt tagtatggga tgatgatttt
     2821 agggatatcg tagattctga actccacaag cagatagttc tgttgattcg gtggggaaac
     2881 catgctgctc atggtggtga aattaaggaa cgagaagcga ttttagcttt gcatcatttg
     2941 tatcagtttg ttaattttat cgattattgt tacagcaatg agtttgtgga gcgttatttt
     3001 gatgagaagt gcttaccact ttcagcaaac atcaaatacc gagaaactcc acaatctatg
     3061 ataaagttac aagacagttt accagaactg cctgattttc atgaacagat ggctgctcag
     3121 tccgtagaag ttcaagagac ttatactgaa aaacgtgaga ctgcagcgca acggcaagat
     3181 gtgcctttcc atattgatca attatctgag gcagagacaa gaaagctctt tattgatatc
     3241 gatctccgtt tagcaggatg gatatttgaa gaaaactgtc gtgttgagat agccgttgat
     3301 ggtctcaagc acggttcagg aattggttac tgtgactatg tactttatgg taaaaatggg
     3361 aaaattttag cgattgtaga ggctaaaaaa gcctctgtca atccagaagt aggggaagta
     3421 caggtcaaag aatatgctga agctcttgag aaacatatcg gctatcagcc aatttgcttt
     3481 attacaaatg gcttgaagca ctatatactt gatggtccga accgccgcca gattgcaggc
     3541 ttttactctc aagaagaatt gcaattagtg atggatagac gtcatcttca aaaaccactt
     3601 gaggatattt ctagtaaaat tagggacgat atttccgggc gtcactacca aaaacatgcc
     3661 attgcaagcg tttgtgaagc tttctctaat catcgtagac aggtactttt ggttatggca
     3721 actggggctg ggaaaactcg tacagcagtt tctctagttg atatcttatc acgtcataac
     3781 tgggtaaaaa acgttctctt cttagccgat agaacttcct tggttaagca agcctatgat
     3841 tcgtttagaa aattactccc agatctttcc gtttgtaact tcttagaaga taaagaagga
     3901 gctcaatcaa gtcgcatggt cttttcaact tatccgacca tgattggagc gattagtggt
     3961 caagaagaag taaatcaacg ccctttcact gttgggcatt ttgaccttat cataattgac
     4021 gaatctcacc gttctattta tcagaaatac aagtccattt ttgattattt tgatgcaaga
     4081 attgtaggct taacagctac tccgcgtcaa gatttagata aaaacaccta tggattcttt
     4141 aatttggaga atggggttcc aacatatgca tatgatttgg aagaggctgt taaagacgga
     4201 tatttagtag cctatcattc tatcgaaacc aaactgaaac tacctacgga tggtctacat
     4261 tatgatgatt tgtccgaaga agaaaaggaa cattttgata gcaaatttga agacgatagc
     4321 tgtgaaaaag atattgatgg gagtgtattt aattcctttg ttttcaataa aagtacagta
     4381 gaaattgttt taaatgaact catgacaaga ggaattcaga cagcctcggg tgatgaaatt
     4441 ggtaaaacta ttatttttgc taaaaatcat gatcatgcgg aatatatcag aggtattttt
     4501 aacaaccgct atcctgaaaa agggagcgac tatgctcagg tgattgatta tagtattaag
     4561 cattatcaga ccttgattga tgattttaaa attaaggaga agtatcctca aattgcgatt
     4621 tctgtcgata tgttagatac aggtattgat gtaccagagg ttgttaattt agtcttcttc
     4681 aagaaagtac gctctaaaac taagttttgg cagatgattg gtcgaggaac ccgtctatgt
     4741 aaagatttat ttggacctga gcaggataag gaaaacttct tggtatttga ttatggggac
     4801 aattttgatt attttcgtgc agatccaaga gatggagagg gtcgtcacat tgtttcgttg
     4861 actcagcgtt tatttaatat caaagtggac ttgattcgag aacttcaggg actccaatac
     4921 caagaagatc agtttgcgag agcataccgt cagcagcttg tctcggaact tcaaggtcgt
     4981 atagagagct taaatgagtt ggacttcagg gttcgtatgg ttttagatac agtttatagc
     5041 tataggaaat tggaaagttg gcagaatcta actgctgtta caagtgaaac cattcaaaaa
     5101 aatctctctc tgcttttatt tgatgaagat aaagaagatg agatggcgag gagatttgat
     5161 ttgtggttgc ttcatattca gttggggcaa ctgacagcta aatcttccac tgttcatatt
     5221 tcccaagtga tgaagacggc tagagctctt tctgctattg gcaatatccc gcaggttttt
     5281 gagcaggctg aaattatcag gaaagtacag gagcctgaat tttggaaaga agttaacttg
     5341 tctgatttgg aaaaaattcg tcttgctatt cgagatttat tacagttttt ggataaaaca
     5401 gaccgtaaac cctactatgt taactttgaa gatcgtatac tctccactgt tcacgagacc
     5461 acagcatttt tgcaggtcaa cgatcttcgg tcttacaatg aaaaagttga gcattatttg
     5521 aaaactcatc tggatgagga gtccatttct aagctatacc ataataaaaa gttgacatct
     5581 gatgatatgc ttgcacttga aaaattgctt tgggaaaaat taggtagtaa agcagactac
     5641 caaagtcatt atgaaaataa ggcaattccg agattggttc gtgagattat tggcttagat
     5701 agagagtctg ccaatcgtat tttttctaaa tttttgtcgg atgagaatct taatgccagg
     5761 cagatttcat ttgtaaaatt gattgtagac tacattgtag aaaatggttt tttagagacg
     5821 aaagtgttaa cgcaagagcc gtttaaatct tatggttctg ttcaactact cttccaacac
     5881 caactaccag tacttcgtaa tattgttcaa atcattgaac ttatcaataa tcgagctgga
     5941 gaagcggctt aaattctaaa gtgattgcca tgctgagact catttaaaat taaaaagagt
     6001 agaaatttat gctatatatg agaagtttta ttaggaagaa tgtcatcgtt ttcctagaat
     6061 acagtatcag ttgttaagtg gttgataaat ttcaaagtag atacttgtac cacgatgttt
     6121 gttgatcgag ttattaacaa aagagctact ttgattttaa agaaatagaa aacaaaaagc
     6181 cgagcaagaa ttcaattgca ggagaaaatg aaataatact caatgaaaat caaagagcaa
     6241 actaggaaac tagctgcagg ctgctcaaaa cactgttttg aggttgtaga tggaagctga
     6301 cgcggattga agagattttc gaagagtata aatcttccta ggataaagca aaacgcatag
     6361 tatcaagggt tttcaacact tgatactatg cgttttctga tgttaaagac tttctaccag
     6421 gttttttaaa agcataattg ttagttgtag tcatttatta ttcttcaaag aaaaatggtg
     6481 gggcgaattt tttcagttct tcaaagcact tttgagcagt atctgcatct tcacagatga
     6541 taagacagac atcattacca caaagggtag cgatagcgtc agggaagctc aaagtatcaa
     6601 tgatagaacc aaaggattga gccagtccag gaagggtttt tagtaggact tggtgttgaa
     6661 ctgggcgcat ccagacaagg gcgtcttcca tgtagagttc gagacgtttt tcccattttg
     6721 agatggaacc attgttaaga acataataag cgctatcttc ttcgcggact tttgataggt
     6781 tcatattttt gatgtcgcgt gagagggttg cctgggttac ttgaatgtcg ttctcagcaa
     6841 gaagggcttg caactcagcc tgtgtatgaa tcttgttttt tgtgataaga gcgcgtataa
     6901 gttggtggcg gtgttctgat ttattcataa taatgtaact ccttttagca aggtaaggta
     6961 agcatggact gagcgaggtc gacagtcaag tggtagtctg tattgtcacg gatggtgatt
     7021 tcaaagtcag tagtatagag gactaaacgg agagtgtctc cttcttttag cttgtaaata
     7081 gttggctgca gttcaaattg aacgtccatc cattcatctg cagtaatatc ctctactaac
     7141 agtaaatcat ttctattttg taaattaagg taaccttttg tcacgactcg ttgtgcctct
     7201 ggtctaaatg gcaattcaca gagattttcc aacatgtgat agcgaccgtt gtcaatggtt
     7261 ctagcactta aaatagctgg ataaggttgt aggtatttct tttgcccaaa ttctagcagt
     7321 tgggcagata agagcccctt gtttgtactg gatttgatac gaagattgag ctgagcgcga
     7381 ccgtttaggt ggagatcttt agtcacagga aggttaatag taatctgatt ggctttccct
     7441 tgatagagct ctgtattgaa ggtttggtat gtcttaccat agcgctcaaa atccttatct
     7501 gggtactggt tttgaatagc ttgctcttct tgaccaagtg agaaggtttc acagttttct
     7561 tgcccaccga agttatcaag tgataaccaa gtctgtggag ctgtattgtc ctgccagata
     7621 acagtaggaa gttgaaagtc tgtttcctgt cctagtaatt tcttggtcaa taaggcattt
     7681 atggactcac ggaagtcaat tgattgccaa ttgttcatgt aaacatgggc accattatgg
     7741 aaaaagagat gcttgtgtat atgagtagga agagcatgga acatctggta aacatgaagt
     7801 ggtttgacat tccaatcctg agaaccatga gtaaagacaa cctctgcctt tactttatgg
     7861 gcattgagca gataattgcg gtcatgccaa aactgattgt agtccccagt ttttcggtct
     7921 agctgagctt tcactttttc taagtcagct tggtgagctt cattgccacg gatatagtcg
     7981 ccagctaaga gattacgaga ataggttaac tcagcaaggg agtcaaagtc ctcacctgga
     8041 taaccacctg ggctagtcac cagaccgttt tcacggtagt agttgtacca tgatgaaatt
     8101 cctgcctcgg caatgataac ttctaaacca tcgactcctg tagtcgcaag accattggac
     8161 atggtaccta gataggaaag tcctgttgta gcaacttttc cgtttgacca atcagccttg
     8221 acttgacgct ggcgcgtgtg atcagtaaag gcacggcaac ggccgttaag ccaatcgatg
     8281 acatttttat aagcctcgat ttgctggtag tctccattag tcatgaaacc tgtcgagtct
     8341 ttggtaccaa cacctgagac atagagattg gcaaagcctc tcggaaggaa gtagtcgttt
     8401 agtgtatagc tagagttgat gtgagttagc ttttcctcag cctctgctat aagctcagct
     8461 ttaccttggg gttggacgag atttagttga ggtttctcta gctcaatctt gtgaggaagt
     8521 ttaacctcaa gctcgccctc catcttgtag agagccttgt cactagcctt gtcattggtt
     8581 ccctgatgat aagggctggc tgtcatgatg gcagggattt ttccatcaaa acgagggcga
     8641 ataatgctaa cctttactag gtctgatagc cctttttggt cagtatcgac acgagactca
     8701 acgtaaacga cttcacgaat gacatcctgg ttagaaaaag tagccaaact cttgccgtta
     8761 aagtagtggt agtcattatc ctccggaata agaccatcac taacaagttg gtcgataaga
     8821 gtatttcctt ttttggtgcg agtattgagt aactgataga gattttcaat caagtcacca
     8881 tatataatgg gaaatccagt ttctttacga aaaacgtcac tatcttcgaa gtcaaccaaa
     8941 taagcaaagc ctaaaagttg aaaagcaaca gtataaaaaa tatctgctgt cagttcatct
     9001 tctgattgaa aaaatgtcag caggtctgtt tttttatcag ctgctaggat agaaagtggg
     9061 tagttggtgt cttgataagt gaaaaagaaa cgacgtaaaa aggtttcaag tgagtctttg
     9121 tgattggctg tattttgtaa atcaaagcca cattttttta gttcagataa gacattttct
     9181 tttggaaaat tgatataact atattgatta aaacgcatag aacctccata tagaatgaca
     9241 gttaaggtta ttatatcaaa aaaaaagcag aaagggaatt gttaacttca aaaggaaata
     9301 atccaataaa aatgaataaa gtactaaatt caatatagag aacagagtaa caataagaat
     9361 aaatagatag ggtataaaag ttctaggaga tttatattat atgctttcta tttttatata
     9421 caatatagta taaatataaa aatgatgaca aaaatacaaa tgaatagaaa ataaattagt
     9481 aagctgatga aatttttctc aagagaagcc atttataggt gaaaatggta taatatagtg
     9541 agaaggatag aggagaagtg taaattgatc gcacaactag atacaaaaac agtctatagt
     9601 tttatggaaa gcgtcatttc gatcgaaaag tatgtgagag cagctaaaga atacggctac
     9661 actcatttgg ctatgatgga tattgacaat ctttatggcg ctttcgactt tctagagatt
     9721 acaaaaaaat acggcattca tcctttgata gggcttgaaa tgacagtgtt tgtagatgat
     9781 caggaagtga atttgcgctt tttagctcta tctagtgtgg gctatcagca gttgatgaag
     9841 ctttcgacag ccaagatgca gggggagaaa acttggtcag tcctgtccca gtacctggag
     9901 gatatcgcgg tcattgtgcc ttattttgat agagttgagt cgttagaact aggctgtgat
     9961 tactatatag gggtttatcc agaaacacta gcaagcgaat ttcatcatcc tatcttacct
    10021 ctttatcggg tcaacgcttt tgaaagcagg gatagagaag ttcttcaagt tttaacagcg
    10081 attaaagaaa atctaccgct cagagaagtt cccttgcgtt cgagacaaga tgtctttata
    10141 tcagcaagtt ctttagagaa actattccaa gagtgttttc cgcaagcttt ggacaattta
    10201 gaaaagctta tttcaggcat ttcttacgac ttggatacta gtctgaaact gcctcgtttt
    10261 aatccagcta gaccagcagt agaggagttg agagagcgtg ctgaactggg gcttgttcag
    10321 aaggggttga ctagtaaaga atatcaagat agactagacc aagaattgtc tgttattcat
    10381 gatatgggct ttgatgatta tttcttggtt gtttgggatt tgttgcgttt tggacgatcg
    10441 aatggctatt atatgggaat gggaaggggt tctgcagtag gcagtttggt ttcttatgcc
    10501 ttagacatca cggggattga cccagtagag aaaaatctga tttttgaacg ctttcttaat
    10561 cgtgaacgct ataccatgcc tgatattgat attgatatcc cagatattta tcgtccagat
    10621 tttatcagat atgttggtaa taaatatggt agtaaacatg cggcacaaat cgttactttt
    10681 tcaacctttg gagccaagca agctcttcga gatgtcttga aacgctttgg tgtgccagag
    10741 tatgaattat ctgcaattac taagaaaatc agttttcgtg acaatcttaa gtcggcctat
    10801 gagggcaatc tccagtttcg tcagcaaatc aatagtaagt tagaatacca aaaagctttt
    10861 gagattgctt gcaagataga gggctatcca agacaaacct ctgtccatgc ggctggtgtt
    10921 gtaattagtg accaagattt aaccaactat attcctctaa agtatggtga tgaaattcca
    10981 ctgactcagt atgatgctca tggagttgag gctagcggac ttttgaagat ggactttctg
    11041 ggactacgaa atttgacctt tgtccagaag atgcaagagt tgcttgctga aatagaaggt
    11101 attcacctta aaattgaaga aatagatttg gaagacaaag aaacgttagc tttatttgcc
    11161 tctggaaata caaaaggtat ctttcaattt gagcaaccag gtgccattcg tctgcttaag
    11221 cgtgtgcaac cagtctgttt tgaagatgtc gtcgcgacta cttctctaaa tcgaccgggt
    11281 gctagtgact atatcaataa ttttgtggca agaaagcatg ggcaggaaga agtgactgtt
    11341 ctggatccag tactggagga tattttggct ccaacctacg gcataatgct ctatcaggag
    11401 caggttatgc aggttgccca gcgatttgcc ggatttagtc ttgggaaagc cgatattttg
    11461 cgtcgggcta tggggaaaaa ggatgcctct gccatgcatg agatgagggc ttcctttatt
    11521 caaggttcat tagaagctgg tcatactgtg gaaaaagcag agcaggtctt tgatgttatg
    11581 gagaagtttg caggttatgg ttttaacagg tcacacgcct atgcctactc agccttggcc
    11641 ttccagttgg cttattttaa aacacattat ccagccattt tttatcaggt catgttaaat
    11701 tcttccaaca gtgattactt aatagatgca cttgaagcag gttttgaagt agcctctcta
    11761 tccatcaaca ccattcccta tcacgataaa attgccaaca aggccatcta tctaggtttg
    11821 aaatccatta aaggagtcag taatgattta gctctctgga ttattgaaaa tagaccttat
    11881 tctaacattg aagattttat agctaaatta cctgagaatt atctgaaact tcctctgcta
    11941 gaacctttgg taaaagttgg tcttttcgat tcatttgaaa aaaatcgtca aaaagtattt
    12001 aataatttag ctaatctatt tgaatttgtg aaagagttgg gaagtttgtt tggagatgct
    12061 atttatagtt ggcaggaatc ggaagattgg acggaacaag aaaaatttta tatggaacaa
    12121 gagcttttag ggataggtgt cagcaaacat ccactacaag ctattgcaag taaggctatt
    12181 tacccgatta ccccaatcgg aaatttgtca gaaaatagct atgctattat cttggttgaa
    12241 gttcagaaaa taaaagtgat tcgtaccaaa aagggtgaaa atatggcctt cttacaggca
    12301 gatgatagta agaaaaaatt ggatgtcact ctcttttcag acttatatcg tcaggttgga
    12361 caggaaataa aagagggagc cttctactat gtaaaaggaa aaatacaatc acgtgatggc
    12421 cgtctgcaaa tgattgcaca agaaataaga gaagcagttg ctgaacgctt ttggatacag
    12481 gtgaaaaatc atgaatcgga tcaagaaatt tcacgcattt tagaacaatt taaaggccca
    12541 atcccagtca tcatccggta tgaagaggaa cagaaaacca tcgtttctcc ccatcatttt
    12601 gtagctaaat ccaatgaatt agaggagaaa ttgaatgaaa tcgttatgaa aacgatttat
    12661 cgctaaaaat acggaaaata gaagaatttt aaaatcaaat gtgatataat caagaagaat
    12721 gttaaaagaa aaaggagcat aaaccaatat gaaacgtatt gctgttttga ctagtggcgg
    12781 agacgcacct ggtatgaacg ctgccatccg tgcagttgtt cgtcaagcaa tttcagaagg
    12841 aatggaagtg tttggtatct atgacggata tgctggtatg gttgccggtg aaattcatcc
    12901 cctagatgca gcttcagtag gggacatcat ttctcgtggt ggtacttttc ttcactcagc
    12961 tcgttatcca gagttcgctc aacttgaagg gcaacttaaa gggattgagc aattgaaaaa
    13021 acacggaatt gaaggtgtag ttgttatcgg tggtgacgga tcttaccacg gcgctatgcg
    13081 tttgactgaa catggcttcc cagctattgg tcttccaggt acaatcgata acgatatcgt
    13141 tggtactgac tttacaatcg gttttgacac agcggttact actgccatgg acgctatcga
    13201 taagattcgt gatacatcat caagtcaccg tcgtactttt gtaatcgaag ttatgggacg
    13261 taacgctggt gatatcgctc tttgggctgg tattgcaact ggtgctgatg aaatcatcat
    13321 ccctgaagca ggcttcaaga tggaagatat cgtagcaagc atcaaagctg gttatgaatg
    13381 tggtaaaaaa cacaatatca tcgtcttagc tgaaggtgtg atgtcagcgg ctgaatttgg
    13441 tcaaaaactt aaagaagctg gagatacaag cgaccttcgt gtaacagaac ttggacatat
    13501 tcaacgtggt ggttctccaa ctgcgcgtga ccgtgttttg gcgtcacgta tgggtgcaca
    13561 tgctgttaaa cttcttaaag aaggtatcgg tggtgttgcg gttggtattc gtaacgaaaa
    13621 aatggttgaa aatccaattc ttggtactgc agaagaaggg gcattgttta gccttactgc
    13681 agaaggtaag attgtggtta acaacccgca caaagccgat attgagctat ctagcttgaa
    13741 taagagcttg tcataattta caatttagtt aataagacca aaaaggtcat atatataaag
    13801 gagtcacaaa aatcatgaac aaacgtgtaa agatcgttgc aactttgggt cctgcggtag
    13861 aaatccgtgg tggtaaaaaa ttcggtgagg acggatactg gggtgaaaaa cttgatgttg
    13921 aagcttcagc taaaaacatt gctaaattga ttgaagctgg tgctaacaca ttccgattca
    13981 acttctcaca cggcgaccac caagaacaag gtgagcgtat ggcaactgtt aaacttgcgg
    14041 aaaaaattgc aggtaaaaaa gttggtttcc ttcttgatac aaaaggacct gaaatccgta
    14101 cagaattgtt cgaaggtgaa gctaaagaat attcatacaa aactggtgaa aaaattcgtg
    14161 ttgcaactaa acaaggaatc aaatcaactc gtgaagtgat tgcgttgaac gttgctggtg
    14221 ctcttgatat ctatgatgat gttgaagttg gtcgtcaagt tttggttgac gatggtaaac
    14281 ttggtcttcg tgtggttgct aaagatgatg caactcgtga atttgaagtt gaagttgaaa
    14341 acgatggtat catcgctaaa caaaaaggtg tgaacatccc taacactaaa attcctttcc
    14401 cagctcttgc tgaacgcgat aacgacgata tccgtttcgg tcttgaacaa ggtatcaact
    14461 tcatcgcaat ttcattcgta cgtactgcaa aagatgtgaa cgaagttcgt gcaatctgtg
    14521 aagaaactgg aaacggacat gttcaattgt tcgctaaaat cgaaaaccaa caaggtatcg
    14581 ataacttaga tgaaatcatc gaagcagctg atggtattat gattgctcgt ggtgatatgg
    14641 gtatcgaagt accgttcgaa atggttccag tttatcaaaa aatgattatc aagaaagtca
    14701 atgctgcagg taaagttgtt atcactgcaa caaacatgct tgaaacaatg actgaaaaac
    14761 cacgtgcaac tcgttcagaa gtatcagacg tattcaacgc tgttatcgac ggaactgacg
    14821 ctacaatgtt gtcaggcgag tctgcaaacg gtaaataccc actcgagtca gtaactacaa
    14881 tggctacaat cgacaagaac gctcaagctc ttcttaatga atacggacgt cttgattcag
    14941 attcatttga gcgtaactct aagacagaag taatggcttc tgctgttaaa gatgctacta
    15001 gctcaatgga tatcaaattg gttgtaactc ttactaagac aggtcatact gcacgtttga
    15061 tttctaaata ccgtccaaat gctgacatct tagcattgac atttgacgaa ttgacagaac
    15121 gtggcttgat gttgaactgg ggtgttatcc caatgttgac agatgctcca tcttcaactg
    15181 acgatatgtt cgaaatcgct gaacgtaaag cggtagaagc aggtctcgtt gagtcaggcg
    15241 atgatatcgt tatcgttgct ggtgtgccag taggagaagc tgttcgcaca aacacaatgc
    15301 gtatccgcac agtacgttaa gaaaaatata aaaacctatc atatccagct ttctagcctg
    15361 tatgatgggc tctttttttg tccagggggc aaaaaggggg caaaactttc ataatggatt
    15421 gatactcttc gaaaatcaaa ttcaaaccac gtcagcgtcg ccttaccgta tatatgttac
    15481 tgacttcgtc agttctatct acaacctcaa agcagtgctt tgagcaacct gcggctagct
    15541 tcctagtttg ctctttgatt ttcattgagt atgaaataag atatgcataa attgattaga
    15601 aagtcaaatg aatttctaca aatgttttag caatcgcaat ctacttgtct agattcgatc
    15661 tgatatattt tcgatttaat gatatggtat ttaaaacctc caaagtagct tgctccattc
    15721 ttttacctac gtgagtctag atgttattta ctgttttagc gtttttgtgt tctactctaa
    15781 ccattatagc attcttctca gctagtgtac taaggagtgt gtgcctgaaa atatgggaac
    15841 taaggggctg gtttatcggt ttctctagtt tagtatttgc cttttgcaaa gtgatcttaa
    15901 atgcctttct ctaaatttac atatcactat tgtttaacaa aatctaatct attttaggtc
    15961 acttattctt tttttgaaat gtagaatgaa ctttttcaaa gtttttcgaa tcttttaaaa
    16021 tctgtttgtt ttatatcgcc attctccccc ttttttaatt ctccctatat agcctgacaa
    16081 cttttccgat ggtacgaata tggttgcttt cgtctaggtg gatgtcggag tattcgggat
    16141 tgagtttttt tgaggtagcc ttggcggagt ttcttgacat agttagtgcc gtctacttgg
    16201 aagatgccga tggtattata gtcaatctgt ggggtattct tgataaatag gtagtcgctg
    16261 tttcttatct ttggctccat ggacttgctg acgacataag cgattgggtc gtagtcgtct
    16321 gggataatgg aaactccata actaaatcgt cgtcctgcat caagcggcta cctgcagaga
    16381 taaactacct aacacgagag taagtagtct gtctgtagtc gtccagtctg atgattttta
    16441 cgatacttcg tttttctgat catacagttg cctctcggca taggtcagaa ctttaccttg
    16501 tctgggtggt gcccgttggt cgtagataga ttggatatcg ctaggagaat ccttttgaac
    16561 tggaggaaag agggcatcaa tcaagctact gaatacttta actaagtcaa atatagtatt
    16621 tttcttagta gacctaaccc ttttttcata atttctaatg gtgtttttac ttatacctat
    16681 cttagtaccc aattcttatt gagtccaacc attactagtc tatattgttt tatagttgat
    16741 tgagtttgga atagtacgct gtagctgcta aaacatttct aggaattaat ttgcctttcc
    16801 taatagagtt gttcatatct tatttcaatc tactatattt ttcacctcta acaatcgcaa
    16861 tctcttcttt atccatgaac gaaaacgctt tctatctttg taagtaaagc atcacacgaa
    16921 atccacgaaa atgaaaacct ttgttgtgtt ttcgtaaaaa atttgttgac agagcacgaa
    16981 acgcctacta taattaaatc aagtttaagg aaataacaaa aaaacaaatc ggagggaaag
    17041 acgatgaaca cattaaatga gaaagtaatc aatatctgta aagcagtagt taaagaaact
    17101 ttaatccaag acatttagga gaaagaaatg aagaaatcaa gaaaattagc cacattagga
    17161 atctgttcag ccttgttttt aggcttggct gcttgtcaac aacaacatgc tacttctgag
    17221 gggacgaatc aaaggcaaag cagttcagcg aaagttccat ggaaagcttc atacaccaac
    17281 ctaaacaacc aggtaagtac agaagaggtc aaatctctct tatcagctca cttggatcca
    17341 aatagtgttg atgcattttt taatctcgtt aatgactata ataccattgt cggctcaact
    17401 ggcttatcag gagatttcac ttcctttact cacaccgaat acgatgttga gaaaatcagt
    17461 catctctgga atcaaaagaa gggcgatttc gttgggacca actgccgtat caatagttat
    17521 tgtcttttga aaaattcagt caccattcca aagcttgaaa agaatgacca gttgcttttc
    17581 ctagataatg atgcgattga taaaggaaag gtctttgatt cacaagataa ggaagagttt
    17641 gatattctat tttcgagagt tccaactgag gcaactacag atgtcaaggt tcacgctgaa
    17701 aagatggaaa cattcttctc acaatttcaa ttcaatgaaa aagctcgaat gctgtctgta
    17761 gtcttgcacg acaatctgga tggcgagtat ctgtttgtag gccacgttgg ggtcttagta
    17821 cctgctgatg acggtttctt atttgtagag aaattgactt tcgaagagcc ctaccaagcg
    17881 attaaatttg ctagtaagga agattgctac aagtatttag gcaccaagta tgcggattat
    17941 acaggcgagg gactggctaa gccttttatc atggataatg ataagtgggt taaactttaa
    18001 tagtgttatg atatgacagt aatcttagga tttttatttc tatttttgat gagttggtgg
    18061 gttatttcaa tcgttaatag ccagaaacca cgcaaaaatg aaacatttat tggctatatc
    18121 caaagatacg atattgatgg gaatggctat caatcattac cgtgtcctca gaactgagag
    18181 gagttcttga aatcgtaaaa ctactttgaa caagagttac ttcaacccat tggctccgac
    18241 ggattaagtt gctttcgtga acaccaaaat cagccgcaat ttcttcataa gtgcggtatt
    18301 ctcgcacata ttgaagagtg gccataagaa ggtcttctag gcttaattta ggttttcgtc
    18361 ccccttttgt gtgtttaagt tgataagcag tttttaatac agctaacatc tcttcaaaag
    18421 tggtgcgctg aacaccaaca agacatttaa atcgtgcatc agttagttgc ttacttgctt
    18481 cataattcat agaactacta taccatattt tgtttcgcag gaagtctaat gctaaaatct
    18541 tatcagtgag aagatttctt aaagtttatt ctttgtagtt tcatctgtat tagtacataa
    18601 tttaacttag agaaaatagg aggactaatc gaatggaatt aaaagatttt acagaaaaag
    18661 aacaggaaat gattaagaaa aggcttacaa tgtctaatat tagtgacaaa gaaactactg
    18721 agaagattct ggcgctagta ccacaagact tgattaagcg aatcccgttt tttgtcagaa
    18781 aacatgctac aacacgtacg attaaacgca tttcaattga acaccctgaa ctctacgctg
    18841 tagctcaaac aagtggtgag attccagaaa aagaacgcga agaattgcgt cagattatca
    18901 caactatctt tgaacaaaag atgaataagc atagtattaa gtagagaatg aaaaaatatt
    18961 ttattggcgg tttgggaaat aatgcctatc atagcaagga ttttcttcaa gaactagatt
    19021 cgcaggtcta ttttctaaat ccatatgaaa agcatcttcg agatgaaaca gaattgaaat
    19081 catggtttaa aaatgagatt gtagaggaag aatctatctg tctgataggc cattctcttg
    19141 gaggagattt agctcgttat ctcacatcgg aatttgaaga agtaaaaaaa ctgattcttt
    19201 tggatggtgg ctatctagat ttagataaga ttttaccttt ggatacagag ttagaggaaa
    19261 ctaaaaatta tatcaaatct caaattgttt tggacttagc tcttcttatt tctaaagaaa
    19321 aatctggagc aaagcattgg tcagaaaata tggagaaagc tgtaagacag tcttatcact
    19381 ggaatgttga gtataataga tatgagttgg ctataaatta tgaaaatata gaagcgatac
    19441 tccgcctacg gagaaaaata caagctttta agagagaagt gggagatacc ttgtttatta
    19501 gtcctcgcta tcctaatgaa gctacatgga gagaggaagc cctaaaagaa ttgccagact
    19561 attttgatac tattttttta gaaaactttg gccatgagct ttatactcaa gcacctaaag
    19621 aaatcgctag tctaatgaat gagtggctcg cttattttct atgagctact tgtattgctg
    19681 gaaatggtat aagatgaaaa tctacaagtc actaattttc atataaccaa aaaacgaaca
    19741 ggctcttttt ctgttattca gaagagaact tgttcgtttt tatcttgatt ttcttaaaaa
    19801 gctctatata catagggatt ttctggttta tcgactttgg taaagctgtc gatttccaag
    19861 gttggaaggt tttgtttgag ttctttatgg tagtgattga ccagttttcc tttggctgaa
    19921 atccaagtgt tgttttcata ctgccgggta ttgcccttgg tcagcaatcc atagacacca
    19981 gaatctgcga tacagtggat aatgccgaat cggaacagaa attgactatt ggcatgactg
    20041 gggtcgttat agacaaagcc tgtaaactgg attgtcttgc cctcaaactc atctggatag
    20101 tcgtagatag cctccatgac ttccatatag ttttcattag tgatctgaat actatcttgg
    20161 gataagtatt tatccgccgc cgttcgcatt tccttttcat aggctgattt tgaaaaataa
    20221 gaactggtat ctggtttcaa atattggctt gtcgtccctt cgcttgtctg aatggctaga
    20281 tccgttcctt ccgatagggg gaaatgataa cctttagcag aaacagtctg agaatccaag
    20341 ctaacagttg ggaaagttaa gccaatgaca agtggaatag ctagaagaga aatactcgtc
    20401 atcttggcca atcggctgtt cagatgactg tgggttttga cttgcttcat ccagatatac
    20461 aattgaacaa tagccaagat aaaggaaagc accatggaaa tataggccag ataggaatag
    20521 tgcatgttga tgtactggtt tagtttgccc gacagatgga ggtaaatagt cagttcaaaa
    20581 tagccagcta aaactaaaaa tcgaatcata ggatgactcc aatcaagaga gaataggcta
    20641 aaacgaccag agtcacaatc gtaatgaagt gactaataaa tcgtgctttc aagtaatttt
    20701 tcatcatgag aatatttttg atatccagca ttgggccaat gacgagaaag gccagaactg
    20761 gagccaaacc gaaactcgag agtagagaag cacctataaa ggcatccgcc tcactacaga
    20821 gcgaaagaag aaaggccaaa agcatcaaga gcaggatggc aaaaagagga gtcgcactga
    20881 tagaggtcag aatccgagtc ggaacgtaga cctgtattat agaagcaaag aggcagccaa
    20941 ataccaaata acgccccgta tcaaaaaatt catcaatggc ctgcacaaag acttgaaaaa
    21001 ctttttttgc agaactcaag taagaaaaat catgctcatg acaagccaga cgattttctt
    21061 tctgaatcgg ttcttgccag aaaaatccta gaaaaattcc tagtattaca gccacaagaa
    21121 tggaacccag agctcgtaat agggcgacat ggaaggagtt gccaaaggca gaataggtcg
    21181 caaaaagaac aatgggattg ataacaggtg ctgtcacaag aaaaggaacg gccgtgtaac
    21241 ttggaacctt tttttccaga aaacgattga tgatggggac gattccacat tcacaagaag
    21301 ggaaaagtat accgacaaag gtcccaaaaa agattctccc ccaacgattt cgagggagaa
    21361 aatgataaac cttgtcaggt gtgatataaa cttcaatcag ccctgagaca atgcttccta
    21421 tcagaacgaa gggaagggct tcaatgataa tggagagaaa aatggctcca gtttgaagta
    21481 cactagatgg gagttgttga aaagcagtca tctagttttt cttttctgct ttgacttttt
    21541 tctctttgtc atcagggaaa gtaacttgtt tcaaatcagg gagtttagca aactcttcaa
    21601 acatcttgtc caagtcgtct cgttttgtaa atttgacagc cataggtgta cctcgattct
    21661 aaattctacc tctattatac tattattttg cggaaaaggg agttttttaa tttgttttcg
    21721 atagttgata cacttcgaaa atctcttcaa actacgtcag cttcgcctta ccgtagta
//
LOCUS       DAVKLX010000037        19801 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKLX010000037 DAVKLX010000000
VERSION     DAVKLX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19801
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.037"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            1..634
                     /locus_tag="VW325_001493"
                     /pseudo
     CDS             1..634
                     /locus_tag="VW325_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(751..1098)
                     /locus_tag="VW325_001494"
     CDS             complement(751..1098)
                     /locus_tag="VW325_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9734864.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(1209..2984)
                     /gene="spxB"
                     /locus_tag="VW325_001495"
     CDS             complement(1209..2984)
                     /gene="spxB"
                     /locus_tag="VW325_001495"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9734865.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(3191..5434)
                     /locus_tag="VW325_001496"
     CDS             complement(3191..5434)
                     /locus_tag="VW325_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9734866.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(5444..5815)
                     /locus_tag="VW325_001497"
     CDS             complement(5444..5815)
                     /locus_tag="VW325_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9734867.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            complement(5826..6221)
                     /locus_tag="VW325_001498"
     CDS             complement(5826..6221)
                     /locus_tag="VW325_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9734868.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      6405..6503
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6587..7378
                     /gene="thiD"
                     /locus_tag="VW325_001499"
     CDS             6587..7378
                     /gene="thiD"
                     /locus_tag="VW325_001499"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9734869.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7588..8220)
                     /locus_tag="VW325_001500"
     CDS             complement(7588..8220)
                     /locus_tag="VW325_001500"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9734870.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(8213..9016)
                     /locus_tag="VW325_001501"
     CDS             complement(8213..9016)
                     /locus_tag="VW325_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9734871.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(9016..9540)
                     /gene="thiW"
                     /locus_tag="VW325_001502"
     CDS             complement(9016..9540)
                     /gene="thiW"
                     /locus_tag="VW325_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9734872.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSATKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(9545..10237)
                     /gene="tenA"
                     /locus_tag="VW325_001503"
     CDS             complement(9545..10237)
                     /gene="tenA"
                     /locus_tag="VW325_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9734873.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(10248..10898)
                     /locus_tag="VW325_001504"
     CDS             complement(10248..10898)
                     /locus_tag="VW325_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9734874.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10900..12285)
                     /locus_tag="VW325_001505"
     CDS             complement(10900..12285)
                     /locus_tag="VW325_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734875.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGIVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(12286..12846)
                     /locus_tag="VW325_001506"
     CDS             complement(12286..12846)
                     /locus_tag="VW325_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9734876.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(13109..13197)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13406..14035)
                     /locus_tag="VW325_001507"
     CDS             complement(13406..14035)
                     /locus_tag="VW325_001507"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9734877.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(14037..14819)
                     /gene="thiM"
                     /locus_tag="VW325_001508"
     CDS             complement(14037..14819)
                     /gene="thiM"
                     /locus_tag="VW325_001508"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9734878.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPVVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(15101..15769)
                     /locus_tag="VW325_001509"
     CDS             complement(15101..15769)
                     /locus_tag="VW325_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9734879.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYWAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(15984..16072)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16324..17460)
                     /gene="lctO"
                     /locus_tag="VW325_001510"
     CDS             complement(16324..17460)
                     /gene="lctO"
                     /locus_tag="VW325_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9734880.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(18085..19575)
                     /gene="lysS"
                     /locus_tag="VW325_001511"
     CDS             complement(18085..19575)
                     /gene="lysS"
                     /locus_tag="VW325_001511"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9734881.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYTNLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(19703..>19801)
                     /locus_tag="VW325_001512"
     CDS             complement(19703..>19801)
                     /locus_tag="VW325_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9734882.1"
                     /translation="TYDLEPMAQQVIPKAAFGYIASGAGDTFTSFQ"
BASE COUNT         5935 a         4341 c         3675 g         5850 t
ORIGIN      
        1 ccacgtccag aacatcctaa gaaagcagac gctcaaacca ttgtcgcgtc taaaaataaa
       61 atctcaattc aagaagacaa gaaagcgctt taaaaccagt agaccttttc ataaggttcg
      121 cttgatgtcc caagatgagg ctggtttcgg tagaatcagt aaactgggat cttgttgggc
      181 tccaatagga gtaggtccac atgtccatag tcactatata cgagaatttc gctattgtta
      241 tggagctgtt gatgcccata caggcgaatc atttttctta agagctggta gatgtaatac
      301 tgagtggatg aacgcctttt tagaagagct ttcacaagct tatccagatg attatttttt
      361 actcgttatg gacaatgcta tatggcataa atcaagtacc ttaaagattc cgactaatat
      421 tggctttgca tttattcctc catacacacc agagatgaag cccattgaac aagtgtggaa
      481 agagattcgt aaacgtggat ttaagaataa agcctttcaa actttggaag atgtcatgaa
      541 tcaactccaa gatattatac aaggactgga gaaggaggtg ataaagtcca tcgttaatcg
      601 gagatggact agaatgcttt ttgaaagcag atgagtatta tatgcaattt ctttatataa
      661 aaagaccgga ttgctccgat ctttcaatag ttcatattct caatttctat tttaaaaata
      721 gctaaggtta acgtcaaatg actacgcgac ctatttcata cgataaaaat caagcactag
      781 accagcaggt ccttgaacta ataaggactc tgttccccaa tcggttacag ttggtccgtg
      841 taaaaccttt ataccaagct cgttcaaccg tttgtagttc tggtctacat cctcaacctc
      901 gatatgaata atgattcctg actgaaagtt ttccaaagga accaaatgat tttgtgacaa
      961 cataaggcag tgactaccaa tcgtaaactg agcaaaacca tcatcagcat aatctgcctt
     1021 tttatccaag atatgctcca agtcagcaca gacttgggga acatttgaaa cgataatatc
     1081 taattgattt aaattcattt actctcctcc ataaaaagac cggattgctc cgatctttta
     1141 aagttctgct ctatgaaaat caaagaataa agtctacaag tttcatattt gattttcggc
     1201 gagaggaatt atttaattgc gcgtgattgc aatccttctt cttccaagaa gagacggaat
     1261 ggtacgagtt cttctgcttc gtatttttcc ttgaaggctt tgatagcttc ttctgagtga
     1321 agttttggat ccaattcaag tacttctact ggaagtggac ggtgttgagt gatgcgagca
     1381 tcgatgacaa cagttttacc ttctttgttc aatttaacag cttctgcaac aactgcatcg
     1441 atgtcttcga tacggtcaac tgtgaatcca acagctcctt gagcttccgc aattttagcg
     1501 tagtcagcgt ttgtgaagtc tacaccaaac aagtgtttgt ttgtatcttc gtatttgttc
     1561 ttgatgaagc cgtactcagc atttgagaag acaaggttga taactggaag gtcgtattga
     1621 acgtttgtga taacgtctgg gtagcacatg ttgaatgctc cgtcacccat gatgttccat
     1681 acttggcgat ctggattgtc tttcttagca gcgataccac caggaagggc aatacccatt
     1741 gtcgcaaaga gtggagatgt acgccacatg ttcttaggtg tcatgtgaag gtgacgagta
     1801 gatgtttgag tagtgttacc tacgtcgatt gagtagatag cgtcttgatc agcatgtttg
     1861 ttgattgcat tgtaaacttg atacaattgc aattcaccct cagttttacc ttcgagtttg
     1921 ttcatgtaat cacgccagtt ttggttgttc ttaacgtttg cacgccacca tggagttgat
     1981 tcaactgggt ttactttgtc aaggatagct ttagctgctt gaccagcatc accaaggatt
     2041 gaagcgtcaa gggcatgacg tttaccaagt ttgtaagggt cgatatcgac ttggatgaat
     2101 ttttcagtgt tcttgaatgc ttcgtaaact tcagcaaatg ggaagtttga accaaggaaa
     2161 agaactgtgt ctgcttcaaa gaccacttcg ttggctggtt tccaaccaac acggtaagca
     2221 gaacctgtca aaccttcata gttccattcg aaagcttcaa agtttttacc agttgtgatg
     2281 attggtgctt tgattttacg tgacaattca gtaatcactt caccagcttt aacaccacca
     2341 aatccagcat agataactgg gcgttcagca ttgttcaaga tttcaacagc tttgtcgatt
     2401 tcaacttcgt tcaaagcagg agcgatgaat gagcgttcgt atgaacctga accgtagtat
     2461 gagttttcat cgatttcttg gaaaccgaag tttactggaa tttcaacaac agctggacct
     2521 tttttagaaa ctgcagcacg gcaggcttcg tcaattactt ttggcaattg ctcagcgtaa
     2581 gctacacgtt tgttgtaaac agcgataccg ttgtacattg ggttttgatt aagctcttgg
     2641 aaagcatcca tgttcaattc gttaactgga cgtgatccaa ggatcgctag gaatggagtg
     2701 ttatccatag ctgcatcgta aacaccgtta atcaaatgag tcgcacctgg accacctgaa
     2761 ccaactgcaa ccccgattga gccgccgaat ttagcttgca taaccgctgc aagagcacct
     2821 gtctcttcgt ggcgaacttg taagaagcgg atatctttgt cttcagccaa agcgtccatc
     2881 aatgagctga gtgttcctga tgggataccg tagattgtat ctacgcccca tgttttcaat
     2941 acgttaagca ttgctgcaga tgcagtaatt ttcccttgag tcataatgat aactctcctt
     3001 caattttttt agacttggag aatacgatta catagaattg gaaacgttct ccaaattttt
     3061 actattccac tgtatcatat ttatgctgac ttttctaaaa atctgctcaa aactctctat
     3121 tctctattct aatacagttt tgaaagtgtc gtcatttctg ttttataaca aaaaaatcta
     3181 gtcattactt ttagtctatt ttactaaaat ttaacagaag ggaactggtc agaacagata
     3241 cagaactaaa ggccatggct agacctgcca attctgggtt gagagccagt ccaacacctg
     3301 aaaagactcc tgctgcaatc ggaattccga caacattgta gataaaagcc cagaaaagat
     3361 tgagcagaat acgatgaaag gttttcttac tcatgtcgaa ggcacgcacc actcctaaaa
     3421 gattattggt tgtcaacacc aaatctgctg actcgatggc gatatctgtt ccagctccca
     3481 tagcaatccc cacatctgct acactaaggg caggagcgtc attgatccca tcaccaacaa
     3541 aggctacttt acctgactgt tgcagtttat ggatttcatg ggctttttct tctggcaaga
     3601 cgcctgcaat gacctcttca attccgattt gatctgcaat agcacgcgcc acaccagcat
     3661 tgtctcctgt cagcatgact gtcccgagac cacgtttttt tagctgactg atggctagct
     3721 tagcattttt cttaggaata tcttgcaaag caagcaagcc tttgatttca ttgtcaacag
     3781 ctaagaacac aactgtctta gcttcttttt ctagttcttc tagtttatct tgataagtat
     3841 tagaaatatc catgccatcc agcattttag catttccaag taaaacttgt tttccattga
     3901 ttcgccctga aacacctttc ccgtgcaagg cctgaaaatt ttcaacagtt tgaaactcaa
     3961 gtccagcttc actcgctcgc ttaacgatag cctcagccag tgggtgttga gaagcatctt
     4021 ccaaggaggc tgccaatcca aacacttcta cttcgtcgcc gatgacatct gttaccacag
     4081 gtttcccttc cgtcaaagtc ccggtcttat caaagacaag ggtttgaact ttctggattt
     4141 cctgtaagac agttccattt ttgaggagaa cccccatctt ggcactacgt cctgtcccca
     4201 ccataagggc tgtcggtgtt gcaagtccca aggcacaagg acaggcgata atcaaaaccg
     4261 ccactccgta gagaagagag gacacaaagc tagctccaag cacaaccaca ctatccctga
     4321 gcaagacgaa ccaaacccaa aaggtcatga ttcctaaaat gacaactact gggacaaaaa
     4381 tccctgaaat cttatccgtc aagtcctgaa tcggcgcacg acttgtctga gctttcttca
     4441 caaaatccac aatctgagcc aaaacagtct ctgagccaac tttttctgct ctaaagacaa
     4501 gcgttccact atgattgatg gttgagccaa tgacagtatc tccaactgtc ttgtccacag
     4561 gcagactctc acctgtcacc atggattcgt caatactaga gacaccttct actacgacac
     4621 catcaacagc aatcttttca ccgggacgca ctcgaatcag gtcgcctacc ttgacttgtt
     4681 ccaaaggaac ttggacataa ctatcatcac tcaagacttc tgcggtttta gcttgcaagt
     4741 ccagtaattt ctccacagct tgggacgtat tttttctcat tttttcctca aaaactgctc
     4801 ccaaaagaac gaaaaagagg ataaatccag cactttcgaa gtaaacaggg agaccagcaa
     4861 agagagcaac taggctatag aaataagcca ctagagttcc cagcgcaacc aaggtatcca
     4921 tgttggcatt gtgcttttta aaactggccc aagcactctg gatatatggc ttacctgcaa
     4981 ctaacataat aggcgttgtt gctagaaagg ttccccaatg catgacttga tgactaatgc
     5041 tacctgccaa catcccaatc atgagaatca caagaggcac agtacagata ctagtaatcc
     5101 aaaaacgttg caggagagat agagattttc gagtcttctc aacgactgta tagcttccct
     5161 tttgcatctt catgccacaa gaaaattcat gtcgccctaa ttcttgaggt gtaaaacgaa
     5221 tgactttctc ctcatctacg ccgattggtt ccaagatacc ttcttcttca aacagaattt
     5281 ccttataaca gtttgaagga gtagcacgat gaaaggtaat ctcagctgga attccctttt
     5341 gaagctggat atgggctgga tgatagcctt tttcagcttg gatacggatt ttttgaatgc
     5401 cattttctaa gcttgctttc acaatttctg tcatagtctc cacctactct acaatcatct
     5461 tgccgtgcat catgttcata ccacaagcaa agctaaactc tccagcctgt tcaggcgtga
     5521 tttccactac atactcttcc cccattggca ggttcgcatg tacaccaaaa tctggaaaaa
     5581 caatttgatc cagacatggt gaaggatcct tgcggtcaaa gacaatgcgg gctggcactg
     5641 atttcttgag gacaatcaac tcaggagtat agcctcccat gacttccact cgaatctctt
     5701 ggtatccgtt tttttgctgg gctttttgtc cagatttttc aggctttttg aaaaaccaaa
     5761 acaagataaa cgcgataagg gcaatacaaa taatggttac aatactattt aacatgacgt
     5821 ctcctttaca tacaattaca tcttacttct gttacagcac ttgatttctt ctctgaaatc
     5881 acagcttcca agtcttccaa gtcagtctga gtaaattcac attctacaat caagtcagcc
     5941 aacaaattcc taatcctacg ggaacaaacc ttgtctttga tatcttggac aagtaaatcc
     6001 cgactttggt ctagagttaa aagcgctgaa taaacaaagg acttgccttc ttttttccga
     6061 gtcaaacact ctttctcaac tagacgagcc aaaagtgtct gaaccgtgga cttggaccag
     6121 tcaaaccgct ctgccaaaac cctaatcaaa tctgtactgg tctgctcccc ctgcatccaa
     6181 ataatcttca tgacctgcca ttctgcatct gaaatctgca ttaccatacc tccaaaatct
     6241 acatttgtca attacactca acagtatact cccaaaatct acatttgtca attatagaaa
     6301 taatattttc ttcgaaaaat agaattttaa tcatttgaaa aacgatttgc agtcaaatat
     6361 tactatataa acaataaaaa tatgctatac taaagaaaaa agaaaacaac cactaggggt
     6421 gcgtaaagct gagattaacg actgttagat ccctctgact caatctaggt aatgctagct
     6481 gatggaagtg gaaatgataa tggggactag cagtcttcta ttgcctttct aaaacagact
     6541 agcttgttct taagaataca aacttcagtt ggttgggagg ttttagatga cttatttacc
     6601 cgttgctttg accattgcag ggactgaccc tagtggtggt gctggcatta tggcagattt
     6661 aaagtcattc caagcgagag atgtctatgg aatggctgtt gtaaccagtc ttgtcgctca
     6721 aaataccaga ggtgttcagc taatcgagca cgtttctcct caaatgttga aagcccaatt
     6781 ggagagtgtc ttttctgata ttccacctca ggctgtaaaa actggaatgt tggctactac
     6841 tgaaatcatg gaaatcatcc aaccctatct taaaaaactg gattgtccct atgtccttga
     6901 tcctgttatg gttgctacaa gtggagatgc cttgattgac tcaagtgcta gagactatct
     6961 caaaacaaac ttactacctc tagcaactat tattacgcca aatcttcctg aagcagaaga
     7021 gattgttggt ttttcaatcc atgaccccga agacatgcag cgtgctggtc gcctgatttt
     7081 aaaagaattt ggtcctcagt ctgtggttat caaaggcgga catctcaaag gtggtgctaa
     7141 agatttcctc tttaccaaga atgaacaatt tgtctgggaa agcccacgaa ttcaaacctg
     7201 tcacacccat ggtactggat gtacctttgc tgcagtgatt actgctgaac tagccaaggg
     7261 caagagtctt taccaggcag ttgataaggc caaggccttt atcacaaaag ctattcaaga
     7321 tgcccctcaa ctcggtcatg gttctggtcc agtcaaccat acaactttta aagattaaga
     7381 aaaaaaactc tctagttccc actttaaggg aattagagag tttttatact cttcgaaaat
     7441 ctcttcaaac tacgtcagct tccatctgca acttcaaaac actgttttga gctgacttcg
     7501 tcagtcttat ctaaaacctc aaggcagtac tttgagcaac ctgcgactag ctttctagtt
     7561 tactctttga ttttcattga gtattaatta ggaaagaatg tcatgcaact ttgttaaaaa
     7621 ggcttgcgtt tttgcctcaa tatcttctgc ttgcatcaaa tcacgtacaa cagctacacc
     7681 agctatgcca gtgcccataa gctgatcaat attctccgaa gtcaagccac caatagcaac
     7741 tattggaatg gcaaccgttt ggcaaattgt tttcaaggtt gaaatcaggg tgatgggtgc
     7801 attttccttg gtggttgtcg gaaaaatggc tcctgtcccc aagtaatctg cacctgattt
     7861 ctccgcttcc agagctcttt taaccgtttt agcggtgaca ccgaggattt tttcaggacc
     7921 caagacttgt cgggcaaccg aaactgggag ttcgtcatct ccaatatgca gaccagcagc
     7981 atcaaccgca agacaaacat ccaaccgatc atcgattatc aagggtacct gataagcatc
     8041 tgttatttcc ttgacttgtt ttgccagttg ataatattga ttggttgtga gatttttttc
     8101 tcgcaattgg actatggtaa cccctgaacg gcaggccgtc tcaacttttg caagaaagct
     8161 ttccacggaa tcttgatagc gattggttac cagatatagt ctaagtgctt ctctattcat
     8221 aaacctctcc tttgatggta tctagccaat tttcatctct tcttaggagc gaaagctgat
     8281 tgagtacttg gtaacgaaat tcttccaatc ccattccttg aacaactatt ttctcagcag
     8341 cgatattgag ataagagact gctaagcaag aagcttcaaa accagtcttt ccttggctga
     8401 gaaaaacggc tgttaaggct ccaaccaaat ctcctgtccc tgttatccag tctaattctg
     8461 cacagccatt tcccagtaca gcgacctgat ttttcgaaac gacgaggtcc ttgggacctg
     8521 tgactaagaa agacatacca tgataggtct gacaccagtc tttcaagact tgaagcaaat
     8581 cctccgtttc ttgatcttta gcactcgcat cgaccccaac gccgtggtgc tttaatccaa
     8641 caagacttcg aatttctgac atgtttcctt taaggaccgt aggtctatag tctaaaaggt
     8701 ctctgactaa gctcttacga atggatgaag ctgttacgcc aaccgcatct actaccattg
     8761 ggagacaaac ttgagctgca taagaagctg ctatacggat tgctttctcc ttctcagctg
     8821 acaaatgccc caaattgatg aagagagcct gactttgctt agtaaaatca agaacctcac
     8881 ggggatcatc tgccatgaca ggtttgcatc ccagagccaa aatcccattt gccagcatct
     8941 cacaagaaat ctcattggtc atacagtgaa tgagggaact agagcctata ggaaagggat
     9001 ttgtaaattc ctgcatcagt ctatcctttc actaaagaaa tatccctgca cttttttaaa
     9061 gaattcctgc ttgattaaaa atcgaaaggc aataaaggaa atcgctgtac caatcaaggt
     9121 tgctccgaaa aatcgaggcg tgtagataaa ccagctaagc ttagtagctg atcctgtaaa
     9181 gagtaccata acaggatagg aaacaataga accaataata cctgttccca caatttctcc
     9241 caaggcagaa aagtaaaatt ttcgaccgta cttataaaag agacctgcta gaagggctcc
     9301 aaaagtcgct cctgtgagag ctaaaggcgg aatcccttga gtcgtcatac ggataaaggc
     9361 tgtcactgta gccatagcca aggcataaac aggtcccatc atgattcccg ctagaatatt
     9421 gactacactg gacatcggtg ccattccctc aatccgaaag ataggtgtaa ggactacatc
     9481 aagggcaatc atcatagata aaatggtcaa tttgtgaact tgtagttggt gctttctcat
     9541 gtttctattc ttctcctttt tctaaagact gtaaatcgct cttccatgtc tggtgttggt
     9601 aagccatctc ccaaaacttg gcttccatat gaacactgat gtggaaggca tctagcattt
     9661 tttgcttatc tgtctcatca ctttctcgat agagctgatt gaccagtgct ccctcctctc
     9721 tgatctgttg ctctaactca tccgtaatat aagtttcaat ccattgttga tagagaggat
     9781 ttggtgatgg tttaagatta agtgatttgc ctatatcatg gtataaccaa ggacaaggaa
     9841 gcaagcttgc aaaagcgatg gctaagttcg gttctgcaaa ttgcctataa atatgagaaa
     9901 tgtaatgata acaggttgga gcgattggat gttgctccat ttcctggtcg ctgatttcca
     9961 attccttgaa aaattgttgg cgaataaata actcaccctc cactaaaccc tgagcatttt
    10021 gtttcaagag tcttttcatc tcttggtttg aagtcttatc agccaaaaga tgatagattt
    10081 ctgagaaagc cttcagatag taggcatcct gaatcaggta atagcggaaa atggcaggtt
    10141 ctaaattccc ctcttgtaat tgtaaaataa agggatgatg aaaggaagcc tgccaagctt
    10201 tcttggataa ttccatcgca atatctgtaa attccataat aactccttta taaaaataga
    10261 ctggtttgaa gcaataaaaa gaaaagcagg tagattaatt ttgttttttt aggaatataa
    10321 aaagtccgat agctattctt caactgtgca tgttcgtcat atccgtgagc agatagagct
    10381 ctcaggtaaa gatggcgcca cctaaagact gtcatcagaa ccttactgta aatcaagggc
    10441 gaccaaaaat gtagttcttg accacgtaat aggcaagctt ctttgaggga cttgatttct
    10501 tgctgaatga gaggaaagga attgaatacc acaatcaagg cataggacca agagcgtgat
    10561 agcccctttt gagccaagta caagagaagc tcttttagtg aaatagagga aacaaagaca
    10621 aggccgatac aaactgtcac aaaggccctc gttccaagca tgactgcctg tgaagcatct
    10681 ccgtgtaact gaactgccca gtagttggca aaagatggta aaatggcaag tatgatcatc
    10741 caagctaaca ttttaaatcg acggtaatag agcataaaga gaatacaaaa tgcgactacc
    10801 gaaagattca gagcaatcga aggaatgaaa gatgtttcca aggataaaat cagcaagaag
    10861 agactgataa tcggtgtctg ggttgctact ttgaccatac tatctcacct ccccttgggt
    10921 attgctactc tgagatgtaa gtggtttggt aatggtcact tctttcacat gccgaagacc
    10981 ctgactagtc atctcaatcc aataatcaac cacagaaatc aaagggtcta aacgatgact
    11041 aatgagcaga aaacttcttc cttgattcct ctcctccaca atccacttgc aaaaataatg
    11101 gcaggctcta tcatccaaac ctgcaaaagg ttcatctagc aagatcacgg aagccttact
    11161 ggtcaagatg gtcaagagct gaagaatttt ttgctgacca ccacttaatt gatagggact
    11221 cttatcgact gcctgctcca aatcaaaata tcgtaaagct tgaaaaatcc gctgatttct
    11281 ttcagaatca ggtccatcta attgaagctc ctctcgcaga ctgactcgga taaactgctt
    11341 ctcagcttcc tgaacaatac ccgtcagatc acggtacaaa ctctttttct ttttcaggac
    11401 tgtccccttc catgcaatgc gccccttata cttttgaaat tgaagaatag accgaaagag
    11461 ggttgatttc ccgacaccat tgtcacccag gatacaggaa atcccttgat agaatgtgaa
    11521 atcagcaatt gaaaagaggg ggcgattacc aagctcacca gtcacacggt tcatatggaa
    11581 taattccggg ctagaagcaa cttcctttga agcaacctgt gtcatctcat aggaagggat
    11641 ttgaaacact tcccttagtt ttccgtctct tagctcaacc atatggtcga tataggcttt
    11701 atagtcagat aaatcatggt cgcacaaaat aactgtcttc ccatcaagag ccaactcttt
    11761 tagaatctcc aatatctcga ttctgctctt gcggtcaatg gaagcgaagg gctcatccaa
    11821 gagatagacc ctaggattca tggcaaagag gacagccagc gctgcttttt gcttttcccc
    11881 acctgatagg tgatgaatgg gacggtgtaa gattgcctcg caacgacatt gctggacaac
    11941 ctctgctatt ttagaatcaa tttcctgaag gtgatagccg atattctcca aggtgaaaat
    12001 cagctcctca aacaagttct ccatggtaaa ttgatgattg ggattttgaa agagaatgcc
    12061 aaccttctgg acacgttcga cgatagaaag ctgactgacc tcactcccat ctatcaggac
    12121 ttgaccgcta tagggaagag aactgacttg ggcaatcatt tgaaagaggc tggattttcc
    12181 tgaaccactg ctcccaacta acaaggtaaa ggcttgcgca tgaaaagtaa aatcaaacgg
    12241 ctcagagaaa attggggact gaatcgctcg tagttccaga cccatctatg cttttcctcc
    12301 agttgcaaac tgatgataga gtttgacaat ggcacgaacc aagatggtac agaagaaata
    12361 aacagaaata aaacgtacca caagcaagga aaggacaaac ggaagggaaa aggcgtagta
    12421 acctaactta atgtattcat agacaaagct aacaagcgta atcccaatac tattagcagt
    12481 tagagagagc caactttcat agcgattctt agttacgata aaaccaaatt cacttcccaa
    12541 accttgaacc aagccagaca aaagagctcc tagaccaaat tggctaccat aaaggacttc
    12601 agcaagcgca gctagcactt ctccaatcgt tgcacttccg actctcggaa caaagatggc
    12661 agcaatgggc gcagccatac accagagacc gaagaggatt tcattggcaa aggcctgcaa
    12721 accaagaggt gttaagagta gactgagaat attatacaca tatcctgaac caacgaaaac
    12781 cccaccaaaa aagatagaca agaaagcaag caagataaca tcttttaact gccatttttt
    12841 caacataaaa aaactccttt ttttaaagaa aagtggggca ctcaagaaga ccgacctaaa
    12901 tactttgtat agcagactga atttagaaca gtacacaaga acactaaaat atttctagaa
    12961 attaatttga attttctaat tgatttgttc gcatcttatt tcaatctact atatcatctt
    13021 catccagttt cgtaaaagaa aaaactctaa ttacagatac gaattagagt tcagcttaca
    13081 agattagaca gttcttttcg acatacgaaa aaaacatttc acatttccct tcgccagtct
    13141 taactgtatc aggttcaatg ggtatcatct cagcctaaag caccccaaat gtctttatta
    13201 tttaattatg tgattattat aacacacatt ttatactagt tcaagaaatt gaactggaaa
    13261 tacagccttg cactcacaaa gacagcagat ctttcttttg caaaaaacaa atgacctgtt
    13321 tgatgaatta gccattcaag ctgaatctgg acatagcttt ttaaaaaagg aaaatcctac
    13381 ttacttagaa tccaaggata gatatctatt gttcactcat ttcccgaaca gttttttcta
    13441 tattttttgc atacgatatt gccgaaatga ttgaaacgcc atccatattg gtctttataa
    13501 tgtctttaat atgtttcgtc tgtatcccac caattgcaac taaaggcatt tgtggcaata
    13561 gttttctcat caattcaaga ccttcataac ctatagcacc accagcatca tcttttgact
    13621 gggtatcaaa tacaggccca acacctacat aatctacata ttcaactttt gattgttgaa
    13681 attcttcttc gtttcttata gaaagaccaa ttattttatc tggcatcaat tttctaattt
    13741 catcaacacc aatatcatct tgacctacat gtacgccatc ggcgtcaatt tccattgcta
    13801 aatctatatc gtcattaacg ataaatggaa cattgtattt tttacaaagt tctttaattt
    13861 ggatagctag ctcaagtttt tctaagcctt ctaaagcacc ctcacctttt tctcgaaatt
    13921 gaaataaggt tataccacct tttaaggctt cctcaacgac tgtatataga ttttttcctt
    13981 ggcaagtagt cgttccacaa ataaaatata gttttagtaa ttctttatga aacatcttac
    14041 ttcactcttt tgaattcctt tacatcttca tctgtaatct cgtataaggc atttataaat
    14101 tcaactttaa atgtcccagg aagatgtcca tttggacgtt tttctgccat ttctccagcg
    14161 atattgtaaa ctaacattgc tgtttctaat gattttaatt cttgaccttt ttccagtccg
    14221 ataaaacttg ctactacagc tcctaataag catcctgtcc caatgacttt cggcatcata
    14281 gcactaccat tatgaatcgt taccacttct ccattaacag caatggcatc cacttcacct
    14341 gttactacta ttggaatatt gaacttctca tttgctgcta gagcaatttc gtcaatatta
    14401 tctacgcccg cactatctac tcctttagat gccacatcta ttcctactaa agaggcaatc
    14461 tctccagcat tccccctaat cgcagctagt ttgtaattgt tgattaaatc atctgctact
    14521 ttttttctat attctcctgc tccacaggct acaggatcta aaactactgg gacattatat
    14581 ttctctgcaa ttttcagagc agcttggtat aatttccaat tttcatctgt caatgttcct
    14641 atgtttatta ataaaccacc agcatacttt aacaaatcct ctaaatctgc tggaaactca
    14701 ctcatggctg gtgaggcgcc cagtgctact aatccatttg ctgtgaaatt ttttactaca
    14761 tcattggtta tacaaatgac caatggtgct ttttctttta ataattttaa acttgtcata
    14821 ttgaaatcct tccttttcac tttatacgat ctactaattt cgatttatct ttagttgaga
    14881 atttttttca tttacattga atgatttata ctcaatgaaa atcaaagagc aaactaggag
    14941 gctaaccgca ggttgctcaa aacactgctt tgagcctgta gataagactg acgaagtcag
    15001 ctcaaaacac tgttttgagg ttgtggatag aactgacgtg gtttgaagag attttcgaag
    15061 agtcttacct catcaaattt gtaaatatca tgagccttct ctagacatcg taaccaatat
    15121 caaaaaaagc taattctaaa gcgactgctt gattccagcg ttgctgaagt tctgtcaaat
    15181 cttctcgatt tttaccgaca cgattgagtt catcaaccag aaattgaacc cactctgcaa
    15241 agaaaggacc tctgtggaga ttgatccatt ccgaatgaat atagacttca ggtagagcca
    15301 aatctttaga accccagtct aaatagagac cttctgcaat gaccagcata accaaaagat
    15361 gggcatagtc tgatgaagcc actgcccaat acattagatc ctgaaaggct tttgttacag
    15421 ggtgcaaagt cacttctaga tagtcattct ctgctacttt taactcttta aaagcctttt
    15481 ggaaataacc atcttcatct gcttcaagaa agcctagttg cttggcaaaa cgaagcttgg
    15541 attcaagttt atctgcgtga gctacgcagg cacccagcat ggataagaag gcatcaaaga
    15601 agtgataatc ttgaatcaga tagtccttta agaccttatt ctcaattgtc cccgcaaaaa
    15661 gttccttaac aaaacgatga ttgattgcag cctgccaatc cttctgactg ctttttaata
    15721 attctccaac agtcaaacct ggctgaaatg catagtcttg tgtttccata tttacttctc
    15781 ctctctttac ttgttagtaa ttaataaaac accaagaaat atcaagcaaa atcgtaattc
    15841 cacttgatcc ttttaaagca catcgagagc atttgcagag agctaactaa acaagcctat
    15901 ccagtttata taaacaaaaa actccaatta caatcaagaa ttagagttga cttacaagat
    15961 tagaccgttc atttcgccat acgaaaaaac gtttcacatt tcccttcgcc agtcttaact
    16021 gtatcaggtt caatgggtat tatctcagcc taaagcaccc caaatgtctc tattatttaa
    16081 ttactgaacc agtatagcaa aaaatgaaag ccctagcaag atatttgacc gaaaaatatc
    16141 tttatatagt agattgaaac tagaatagta cacctctact tctaaaacat tgttagaaat
    16201 cgatttgact gtcctgattg atttgtccta ttcttatttc attttactat agttttcgat
    16261 agcaatttat tcttccaata cacgaagaaa aacctccaca ttcagtggag gcaatctgtt
    16321 ttatcaatac aattttaagt cacgagggtc aactgggaag gttgggttgt atggattgtg
    16381 acggagcttg aagtgtttga catcttcaat ggtctgagct ccagacaatt gcataactgt
    16441 cttcaattcc gcattcaagt gttcaaagac ttgacgcaca ccgacactac caccgagagc
    16501 caaaccatag atgacagggc gtccaatagc aaccaagtct gctcctgatg ccaaggcttt
    16561 aaagacgtgt tgaccacgac gaacaccaga gtcaaagaca atcggcacac gtctatcaac
    16621 tgcttctgcc acttcttgaa gcgagtcaaa ggcagctggt ccaccgtcga tttgacgacc
    16681 accgtggttg gttacccaga taccagaagc tcctgcagca agcgaacgtt caacgtcctc
    16741 acggcattgt ggtcccttga catacacagg aagaccagag tattcagcga taaattctac
    16801 atcgcgtgga gacaagcgtt gtttagctga tttgtaaaca aagtccattg atttaccagc
    16861 accttctggc aggtattctt caacaatcgg catgccaact gggaagacaa aaccattacg
    16921 cttatccact tcacgattcc cccctacagt agcatctgcc gtcaagacaa tcgctttata
    16981 accttcagcc ttcacacggt ccatgatgtg gcggttgata ccgtcatcct tactaaagta
    17041 aaattgaaac caatgaggtg tcccttgaag ggcttcagaa atctctggaa ggtcaacagt
    17101 agagtaagaa ctggttgtat aaagagaacc aaactcatgc acaccacgcg cagtcgccac
    17161 ttccccctgt tcatttgcca atttatgagc cgcaacaggc gccataatga ttggtgaaga
    17221 tagtttttca cctgcaaatt caatctctgt acttggattt tcaacatcgc aaagtgtatg
    17281 aggaacgatg agtttgtggt taaaggcgcg gatattctct cttaaagtga aagtatcttc
    17341 cgccccacta gcgatatagc caaatgctgc tttaggaata acttgttgcg ccattggctc
    17401 caaatcatag gtattgatga agtctacatg accttctgca ttgcttgttt tgtatgacat
    17461 aaaatgccct ccttgattaa gtaagcgttt gctttttact acacaactat aataactctt
    17521 ttttcaatca ttttcaagag ttttgcttaa aaaccatctt attttttcat acaatatact
    17581 agccctactt tccttaaact ctaaacttta gtctatatta agtgaatcgt tgagattttt
    17641 tgctgccagg catttgaact acttttttat ttgctgattc tatctactaa tactataaaa
    17701 aagcaagtga ataagacacc agaacatcct ctaataggac ctatggactt atatcagtcc
    17761 catatatact gtttaagcaa gatactatcc tagttgacgc taacttccta tcccacttat
    17821 taatttttta tttatagtga atctgaacaa atcaattggg gaatccaagt caatttctag
    17881 tactttttga acagtttcag cgtactatcg taaaatgaaa taaaacatgc acaaattgat
    17941 tagggaattt aaaccaattt ctaacaatgt cttagaaatc aaagtgtact atttcaactt
    18001 caatatacta cacttcattt tctccttaaa ttcagtctct ttttgaatca aaaaatcctc
    18061 tggtaagagc cagaggataa gaatttattt cattgttggg aagagcaata catcacggat
    18121 agttgttgta tcagtgagga gcatgcagag acggtcgata ccgattccca aaccacctgt
    18181 tggtggcata ccgtattcaa gagcctcgat gtagtcatag tcgatgcctg tcgcttcatc
    18241 atcaccaagt tctttagctt tagcttgggc ttcaaaacgg ctaagttggt cgattgggtc
    18301 gttcaactca gtaaaggcat taccgtactc cttagtcatg ataaagagct cgaaacggtc
    18361 agtaaagcgt tgatcttcag gatttttctt agcgagtgga gatacagcta ctggatgtcc
    18421 atagacaaag gttggttgga ttaaagtttc ttcaacaaac tcttcaaaga aggcattgat
    18481 gatgtgacca acctcagtgt agtgtttctc aactggaact ttcttctcag cagcgatagc
    18541 tttagcttct tctaaagtca tgtcttgcca gaaatcgaca ccagtaattt ctctgatagc
    18601 atccaccata tgaacacgct taaatggttc gttaatcttg atttcagtac cttggtagtt
    18661 gactgggcca tcgcccttaa ctgatttagc agcgtgttgg ataatgcctt ccgtcaagtc
    18721 catgatgtct tggaagtctg cataagcttg gtaaacttcg atagaagtaa actcagggtt
    18781 atgagtagcg tccattcctt cgttacggaa gatacggcca atttcataga cacgttccat
    18841 accacccacg ataaggcgtt ttaagtgaag ctcagtcgcg atacgaagca ccatgtcaat
    18901 gttttgggca ttgtggtggg tgataaatgg acgggcagca gcaccaccgg cttcattatg
    18961 aagaacaggt gtttccactt caaggaatcc tttttggtca aggtaacgac ggatttcaga
    19021 gatgattttt gaacgagtga caaagcgttc aaagctttca cggttagaaa tcaagtcaag
    19081 gtaacgttta cggtaaattg tttcaacgtc tgtcaaacca tggaatttct caggaagagg
    19141 acgaagagcc ttagacaagt gtgtgatgtg ggttgccttg atagagagtt ctcccatatc
    19201 cgtacgcatc acttcacctt cgacaccaag gaagtcacca aggtctgctt ttttgaagat
    19261 ttcgtagttt tcttcaccga cagcatcctt acgaacgtag atctgaatct ggccttcgcg
    19321 gtcttgaagg tgggcaaaac caacttttcc tttaccacgt ttggttacca agcgtcctgc
    19381 gatagtagct gtttcgtttt tatcgtgtaa ttgttcttta tcgaggttgg tatatttatc
    19441 ttttaattct tgtgaatttg cagtacgttc aaaacgtttt ccgaaaggat cgattccttg
    19501 ttcgcggagc gcagccattt tttcacggcg aacgatctgc tggtcattta gttcttccat
    19561 atgttctgta gacatgggat cctcctagtt ttactcaaaa ttgaatacga tgagtcattt
    19621 tttgcgatac tcccatttta tcataaataa gcaagaaatt cacggataat ctttaaaaac
    19681 taaaaagaac ctgacgctaa aatcactgga aagaagtgaa agtatctccc gccccactag
    19741 cgatatagcc aaatgctgct ttaggaataa cttgttgcgc cattggctcc aaatcatagg
    19801 t
//
LOCUS       DAVKLX010000038        19668 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKLX010000038 DAVKLX010000000
VERSION     DAVKLX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19668
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.038"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            172..510
                     /locus_tag="VW325_001513"
     CDS             172..510
                     /locus_tag="VW325_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734883.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
     gene            576..2558
                     /locus_tag="VW325_001514"
     CDS             576..2558
                     /locus_tag="VW325_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734884.1"
                     /translation="MKLKLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIQGKS
                     NLSNNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTL
                     GKMDVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRT
                     RQIKEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFF
                     SLLLTNFILFQIIDLITFVLFWLTIQIEKPIEIIKNKAKNKLIFVVWLAIISIIILVS
                     GVFLQETKSSQSSINIQIQNLVPWDTVKDWRRIEFLGIESNSTKNREVNDSDGQYLQI
                     VAALKNLDFLYIERSSAYVPDFMKTSHVIENFSKQLENDGITNPEINKELIYINQTGA
                     NLQNKVNGTNYHLLDNKIATIYIPEKWKENQKSIENTVVAEQFIGTNYTREQLAVQII
                     PDGEKIFYFNEDADNNLKMKDILPLANVADSKDNIVVVLDTDKMMENNKFSLASNILY
                     KSLFSPEAVKKINEMTVLLNFSMNPVDVYQIVKLKIQSLEHQILLSQILQKIIYSIVF
                     ILIYQYVQLFITLKQNEYVKKIILGLSKTYIAISSLKYFMMTITMVILFTFLMTGQIE
                     LLYIGAASLLVLMLSIIMSFRKLSESYTKILKGDES"
     gene            2555..3178
                     /locus_tag="VW325_001515"
     CDS             2555..3178
                     /locus_tag="VW325_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9734885.1"
                     /translation="MTIDLLNVSKSFGSKKIFTDLNLIFESGKSYALIGGSGSGKSTL
                     LNIIGRLEKIDSGNVLVDKQDIWKIKERTFFKNTVGYVFQNYSLIDNKTVYDNLSLIT
                     KDKKTITDVLEKVGLSSDYLHQKIYELSGGQAQRVAIARMLMKPRKIILADEPTGALD
                     GEIGKEIIRLLLNETAEDKYVIIATHDPAVYNEVDVIIDMKDIGYKV"
     gene            3175..3675
                     /locus_tag="VW325_001516"
     CDS             3175..3675
                     /locus_tag="VW325_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734886.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFDYGKEELNSQKKDIKELYKKIYES
                     FKEKNK"
     gene            complement(3841..5187)
                     /gene="gor"
                     /locus_tag="VW325_001517"
     CDS             complement(3841..5187)
                     /gene="gor"
                     /locus_tag="VW325_001517"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9734887.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            5452..6651
                     /locus_tag="VW325_001518"
     CDS             5452..6651
                     /locus_tag="VW325_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9734888.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            6635..7336
                     /locus_tag="VW325_001519"
     CDS             6635..7336
                     /locus_tag="VW325_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9734889.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            7338..8597
                     /locus_tag="VW325_001520"
     CDS             7338..8597
                     /locus_tag="VW325_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9734890.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            8721..10718
                     /gene="metG"
                     /locus_tag="VW325_001521"
     CDS             8721..10718
                     /gene="metG"
                     /locus_tag="VW325_001521"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9734891.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            10762..10842
                     /locus_tag="VW325_001522"
                     /pseudo
     CDS             10762..10842
                     /locus_tag="VW325_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            11139..11459
                     /locus_tag="VW325_001523"
     CDS             11139..11459
                     /locus_tag="VW325_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9734892.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            11463..12278
                     /locus_tag="VW325_001524"
     CDS             11463..12278
                     /locus_tag="VW325_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734893.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            12353..13279
                     /locus_tag="VW325_001525"
     CDS             12353..13279
                     /locus_tag="VW325_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9734894.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            13345..13611
                     /locus_tag="VW325_001526"
     CDS             13345..13611
                     /locus_tag="VW325_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9734895.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            13604..14302
                     /locus_tag="VW325_001527"
     CDS             13604..14302
                     /locus_tag="VW325_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9734896.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDAYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEARI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            14313..14792
                     /locus_tag="VW325_001528"
     CDS             14313..14792
                     /locus_tag="VW325_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9734897.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            14800..15528
                     /locus_tag="VW325_001529"
     CDS             14800..15528
                     /locus_tag="VW325_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9734898.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            15570..16439
                     /locus_tag="VW325_001530"
     CDS             15570..16439
                     /locus_tag="VW325_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9734899.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            16414..16854
                     /locus_tag="VW325_001531"
     CDS             16414..16854
                     /locus_tag="VW325_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9734900.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            16977..19523
                     /locus_tag="VW325_001532"
     CDS             16977..19523
                     /locus_tag="VW325_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9734901.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
BASE COUNT         6141 a         3330 c         4255 g         5942 t
ORIGIN      
        1 tgtgttcaat tttaaaagga ggggtagtta tgagatattt taacgactct agataccata
       61 acggtgatta aaaacctaaa tataatcaat tcatattaaa gtaatatgga gggactaaaa
      121 agagactatt atttaatctt catcaaatat ttaaattcaa ggagaagtaa aatgaaaaaa
      181 agaattaata agaaagcatc tgtttttcta gttgcaagtg ttttcagttt tagctttgct
      241 actacagcta gtgtcgttaa cgcagtaacg gcacaccctg ctccaagtcc cattattgat
      301 gtatgggaat atggtgtaaa tgataactat ggttattcga attactttgt tagttctcca
      361 agtaatattg gatcaaaatc ttcagtaacg aatttttggg gaacagttaa agctcaggat
      421 aaaaaagatt atggatgggc atattcaagt gcaacaaaat cttggaacga tgttcgctta
      481 aatgcatatt gggattatta tagattttaa aaataattta tgaaaacaag agacccaatt
      541 ataagattgg gtctcttgta atatggtaat tgcttatgaa attaaaatta tgtatcattg
      601 gattcttctt ttgtttgatt gctacaattg gtctggtcac tattagtgat accgaaatcc
      661 ccataccttt accaattgat ggagcttttt ctatccaagg aaaaagtaat ctttctaata
      721 acgaaatata cgaaatggtt cgtgacttat caaaaacgga aaaggttacc atttacaagc
      781 caatcgttca gagttcgggt cagttgaagt atgtaaattt tgatgatgta aataatgaac
      841 aattaaagtc agcaccaata gtagggatgt attacacact tgggaaaatg gatgtagaca
      901 gtttgaaacc acttacgatg acgggactac aaacagtcta tatggcttat ccttggtata
      961 taggaggaat attacaattc actggaactt taagaatact gttaatgggt tcaatttatt
     1021 taactttatt agttgtttta tttgttgtta ggacgaggca gatcaaagaa ggagtgatac
     1081 gacgttcttt gggcttgcct atatacgact taagaagaga gtatggcatt tctcttattt
     1141 ttgaactgat tatgatggct ttattgatga tttcttacag ttcttttttg ggtaatggtt
     1201 tcttcaccta tagttctaag ttatttttct ctctgctttt aacgaatttt atactatttc
     1261 aaatcattga tcttattact tttgttttat tttggttgac gattcagatt gaaaagccta
     1321 ttgaaattat taaaaacaag gcaaaaaaca agcttatttt tgtcgtatgg cttgctatta
     1381 tttcgattat catccttgtc tcaggagttt ttttacaaga aacaaagagt agtcaatcta
     1441 gtattaacat acagatacag aatttagtac catgggatac agtaaaagac tggagaagga
     1501 ttgagttcct tggaattgaa agtaactcta ctaaaaatag agaagttaat gattcagatg
     1561 gtcagtatct acagatagtt gctgccttaa aaaacttaga tttcttatat atagaacggt
     1621 cttcagcata tgttccagat ttcatgaaaa cttcacatgt tatagaaaat ttttctaaac
     1681 aattagaaaa tgacgggata acgaatccag aaataaataa agagttaatt tatatcaacc
     1741 aaacaggtgc taacctacaa aataaagtga atggaacaaa ctatcatctt ttagataata
     1801 agatagcaac catctatatt cctgagaagt ggaaagaaaa ccagaaatct attgagaata
     1861 cagttgtagc agaacaattt attggaacaa actatacaag agaacagctt gcagttcaaa
     1921 taattcctga tggggaaaaa attttttatt ttaatgagga tgctgataat aatcttaaaa
     1981 tgaaagatat tttaccgctg gcaaatgtag cagatagcaa agataatata gttgttgtgt
     2041 tagatacgga caaaatgatg gaaaataata agttttcttt ggctagtaac atactatata
     2101 aatctctttt tagtcctgaa gcggtaaaaa aaataaatga gatgactgtc ctgttgaatt
     2161 tctcaatgaa tccagtagat gtttaccaaa ttgtgaaatt aaagattcaa tccttagagc
     2221 accaaatttt actttcacaa atcttgcaga agataattta cagtattgtc tttatactca
     2281 tttatcaata tgtccaactt tttattactc taaaacagaa tgaatatgtg aaaaaaatca
     2341 ttttgggtct gtcaaaaaca tatatagcaa tttcaagtct caaatatttt atgatgacta
     2401 ttacaatggt tattctattt acatttctta tgacagggca aatagagttg ctatatattg
     2461 gagcggcttc tctgctagtt ttgatgttgt caatcataat gagttttaga aaattatctg
     2521 aaagctacac taaaatttta aaaggagatg aatcatgaca attgaccttt tgaacgtttc
     2581 aaaaagtttc ggctcaaaga agatctttac agacttaaat ttaatatttg aatctggaaa
     2641 aagctatgca ttgattggag gttctggctc cggtaaaagt acccttctta atattatagg
     2701 aagattagaa aaaattgaca gtgggaatgt tttagttgat aaacaagata tttggaagat
     2761 aaaagaaaga acttttttta aaaatactgt tggatatgta tttcagaatt attctttaat
     2821 agataacaaa acagtgtatg ataatctgag ccttatcact aaagacaaaa aaacaataac
     2881 tgacgtactt gaaaaagtag gactgtcaag tgactattta caccaaaaaa tatatgaatt
     2941 gtctggaggg caggctcaac gtgtagctat tgccagaatg ttaatgaaac cacgtaaaat
     3001 tatcttagca gatgaaccta caggagcttt agatggtgag attggaaaag aaattattcg
     3061 tttactattg aatgaaacag ctgaagataa atatgtcatt atagcgacac atgatccagc
     3121 tgtctataac gaagttgatg tgataattga catgaaagat atagggtata aggtatgaaa
     3181 aagaaaattt atattgcttt gatctttgta actggggtac tcgccatttt cttttttgga
     3241 aaacaaatga ttacaaaaga aaatatcaat aagccaacgg tagagttaac aatctatacg
     3301 ctttcttcta gtgatacaga aaaatggaat aaagtgagac aagtagaaac ggaagaagct
     3361 atatacttca taactgtgaa agaagtatca tcttcagaag aagtattttc aaacatcatt
     3421 gcaaatggag ctgctacggg gttcggagtt cgtgaagaag aagtgaagaa attcaataat
     3481 ggtttaggag acaccataga ggactctaag cataataaac taattgaaat agaatttttt
     3541 actttttctg atgatggcgc gggatttgtg gtagcaaact ttgactacgg taaagaagag
     3601 ttgaattctc agaaaaaaga tataaaagaa ttatataaaa aaatatatga atcttttaaa
     3661 gaaaagaata aatgattgta ttataatcct tgtattttgc tatggtttgt taattgattt
     3721 ttgcatatct tacttcaaca tactataaaa taaaacataa gaaaacgagt atctccaact
     3781 acggaaatac tcgctttttg atatttagaa gtgttttttg ctttgcttct tttgtttcga
     3841 ttaacgcatg gttacaaatt cttcagatgc agttgggtga attgccacag ttgcatcaaa
     3901 gtcagccttg gttgctccca ttttgatagc aacggcaaat ccttgaatca tttcatcaac
     3961 gccgtagcca attccatgaa gtccgacaac tttttcttct gaaccagctg ttatgagttt
     4021 gaaacgggat tcttgacggt tgcgagtgca agcagagtac atagatgcaa agcttgattt
     4081 ataaaccttg atttggtctt gaccgtattc tttaatagct tgctcttctg tcaaaccaac
     4141 agttccgata gcagggtgtg aaaagacaac agttggaata gttgagtaat ccatttttgc
     4201 agtagttttt ccgttaaaga gacgttcaga tagggtacgt ccggccttga ttgcaactgg
     4261 agtcagttct ttctcgcccg ttacatcacc tagagcatag attccctcaa caacagtatt
     4321 ttggtattca tccacttgga taaagccacg ttcgttcaga gtcactccag ctttttcaag
     4381 ttgcaagccc ttaacgtttg gacggcgacc tgtagcccag ataacttggc tagctgtgtg
     4441 actagtacca tcttcgaaat gaatggtaat gccgtcagta gttttttcta acttgacagg
     4501 gactttgtga gtgtgaagtg gtaagtttgt tctttccatt tccttgacca aaccttcaac
     4561 gatgtaggaa tcaaaaccac gtaaaggacg atcgcggcga acaaagagat ctgtcttgac
     4621 accaaaagtg tggagtacgc cagctaattc aacggcgata taaccagcgc ctagaatggc
     4681 aattgactct ggaagttctt cccaggcaaa tacatcatca gaagagccac ctagctcagc
     4741 accaggaata ttaggaatac ttggatgggc acctgtagca atcacgatat gtttagcacg
     4801 aatcagttca ccatttacgc ttacagtatg agaatctaca aattcagcat gaccttcaat
     4861 caagtctaca ccgttgcgtt taaaactacc atcataagaa gaacgagcgc gatcaatgta
     4921 ggcttcacga ttgcgacgta gggttgcaaa gtcaaagtta agatcagtag tcttaaagcc
     4981 gtagtcttct ccaaattgat ggaaagtctc agcgatttgc gccccgtacc acatgatttt
     5041 tttaggaaca caaccgacgt tgacacaggt tccacctaat ttcttttcct caataacggc
     5101 tgctttggct ccatgttcac cagcacggtt catggtagcg attcctccgc tacctccacc
     5161 gatagcaatg atatcatatt ctctcattga aaactccttt gtaatctttt aaatagtaga
     5221 gcgtcttttt tgatagttat attttattct ttttttgatt tgattgcaaa aatctgctac
     5281 gcacaaaaaa ttgcaaaaaa gcttgccaaa taagtcaaga tagtgtatta tatatctagt
     5341 acaatagaaa agcaaaattc cgaacgattg tttaatcctg aactgtattg tttgctggag
     5401 ggaaatttgg tataatagca aagtattgta aaaaagaaaa ggagtttcat tatgaagaaa
     5461 aagaatggta aagctaaaaa gtggcaactg tatgcagcaa tcggtgctgc gagtgtagtt
     5521 gtattgggtg ctggggggat tttactcttt agacaacctt ctcagactgc tctaaaagat
     5581 gagcctactc atcttgttgt tgccaaggaa ggaagcgtgg cctcctctgt tttattgtca
     5641 gggacagtaa cagcaaaaaa tgaacaatat gtttattttg atgctagtaa gggtgattta
     5701 gatgaaatcc ttgtttctgt gggcgataag gtcagcgaag ggcaggcttt agtcaagtac
     5761 agtagttcag aagcgcaggc ggcctatgat tcagctagtc gagcagtagc taaggcagat
     5821 cgtcatatca atgaactcaa tcaagcacga aatgaagccg cttcagctcc ggctccacag
     5881 ttaccagcgc cagtaggagg agaagatgca acggtgcaaa gcccaactcc agtggctgga
     5941 aattctgttg cttctattga cgctcaattg ggtgatgccc gtgatgcgcg tgcagatgct
     6001 gcagcgcaat taagcaaggc tcaaagtcaa ttggatgcaa caactgttct cagtacccta
     6061 gagggaactg tagtcgaagt caatagcaat gtttctaaat ctccaacagg ggcgagccaa
     6121 gttatggttc atattgtcag caatgaaaat ttacaagtca agggagaatt gtctgagtac
     6181 aatctagcca acctttctgt aggtcaagaa gtaagcttta cttctaaagt gtatcctgat
     6241 aaaaaatgga ctgggaaatt aagctatatt tctgactatc ctaaaaacaa tggtgaagca
     6301 gctagtccag cagccgggaa taatacaggt tctaaatacc cttatactat tgatgtgaca
     6361 ggcgaggttg gtgatttgaa acagggtttt tctgtcaaca ttgaggttaa aagcaaaact
     6421 aaggctattc ttgttcctgt tagcagtcta gtaatggatg atagtaaaaa ttatgtctgg
     6481 attgtggatg aacaacaaaa ggctaaaaaa gttgaggttt cattgggaaa tgctgacgca
     6541 gaaaatcaag aaatcacttc tggtttaacg aatggtgcta aggtcatcag taatccaaca
     6601 tcttccttgg aagaaggaaa agaggtgaag gctgatgaag caactaatta gtctaaaaaa
     6661 tatcttcaga agctaccgta atggtgacca agaactgcag gttctcaaaa atatcaatct
     6721 agaagtgaat gagggtgaat ttgtagccat catgggacca tctgggtctg gtaagtccac
     6781 tctgatgaat acgattggca tgttggatac accaaccagt ggagaatatt atcttgaagg
     6841 tcaagaagtg gctgggcttg gtgaaaaaca actggctaag gtccgtaacc aacaaatcgg
     6901 ttttgtcttt cagcagttct ttcttctatc gaagctcaat gctctgcaaa atgtagaatt
     6961 gcccttgatt tacgcaggag tttcgtcttc aaaacgtcgc aagttggctg aggaatattt
     7021 agacaaggtt gaattgacag aacgtagtca ccatttacct tcagaattat ctggtggtca
     7081 aaagcaacgt gtagccattg cgcgtgcctt ggtaaacaat ccttctatta tcctagcgga
     7141 tgaaccgaca ggagccttgg ataccaaaac aggtaaccaa attatgcaat tattggttga
     7201 tttgaataaa gaaggaaaaa ccattatcat ggtaacgcat gagcctgaga ttgctgccta
     7261 tgctaaacgt cagattgtca ttcgggatgg ggtcatttcg tctgacagtg ctcagttagg
     7321 aaaggaggaa aactaagatg cagaatctga aatttgcctt ttcatctatc atggctcaca
     7381 agatgcgttc tttgcttact atgattggga ttattatcgg tgtttcatca gttgttgtga
     7441 ttatggcttt gggtgattcc ctatctcgtc aagtcaataa agatatgact aaatctcaga
     7501 aaaatattag cgtctttttc tctcctaaaa aaagtaaaga tggttctttt actcagaaac
     7561 aatcatcttt tacggtttct ggaaaggaag aggaaattcc tgttgaaccg ccaaaaccgc
     7621 aagaatcctg ggtccaagag gcagctaaac tgaagggagt ggatagttac tatgtaacca
     7681 attcaacgaa tgccatcttg acctatcaag ataaaaaggt tgagaatgct aatttgacag
     7741 gtggaaacag aacttacatg gacgctgtta agaatgaaat tattgcaggt cgtagtctga
     7801 gagagcaaga tttcaaagag tttgcaagtg ttattttgct agatgaggaa ttgtccatta
     7861 gtttatttga atctcctcaa gaggctatta acaaggttgt agaagtcaat ggatttagtt
     7921 accgggtcat tggggtttat actagtccgg aggctaaaag gtcaaaaata tatgggtttg
     7981 gtggcttgcc tattactacc aatatctccc ttgctgcgaa ttttaatata gatgaaatag
     8041 ctagtattgt ctttcgagtg aatgatacca gtttaacccc aactctgggt ccagaactgg
     8101 cacgaaaaat gacagagctt gcaggcttac aacagggaga ataccaggtg gcagatgagt
     8161 ccgttgtatt tgcagaaatt caacaatcgt ttagttttat gacgacgatt attagttcca
     8221 tcgcagggat ttctctcttt gttggaggaa ctggtgtcat gaacatcatg ctggtttcgg
     8281 tgacagagcg cactcgtgag attggtcttc gtaaggcttt gggcgcaaca cgtgccaata
     8341 ttttaattca gtttttgatt gaatccatga ttttgacctt gttaggtggc ttaattggct
     8401 tgacaattgc aagtggttta actgccttag caggtttgtt actgcaaggt ttaatagaag
     8461 gtatagaagt tggagtatca atcccagttg ccctatttag tcttgcagtt tcggctagtg
     8521 ttggtatgat ttttggagtc ttgccagcca acaaggcatc gaaacttgat ccaattgaag
     8581 cccttcgtta tgaatgagat caacaagatg gacactcgtc tatcttgttt ttgtatggat
     8641 ttccctatcg aaattcatta aaaatgtgat ataatgaagt gttagaaaaa caggtttcat
     8701 ttgcctggaa aggaaaaatt atgtctgaaa aaaattttta tattacaacg ccgatttact
     8761 atccatctgg gaaacttcat atcggttctg cctacacaac tatcgcatgt gatgtcctag
     8821 cacgttacaa acgcctgatg ggctacgatg tattttatct gacaggtctt gatgaacatg
     8881 gtcagaaaat ccagcagaaa gcggaagaag ctggtattac acctcaagcc tatgttgatg
     8941 gaatggcggt tggagttaaa gaactctggc aattactaga tatctcatac gataaattta
     9001 tccgtacaac tgatgactac catgaaaaag ttgtcgcaca agtctttgaa cgcttacttg
     9061 ctcaagatga tatctacttg ggtgaatact ctggttggta ttcagtatca gacgaagaat
     9121 tctttacaga aagccagctg gcagaagttt tccgtgatga agctggaaat gtgactggtg
     9181 gtattgctcc atcaggtcat gaggttgaat gggtttctga agaatcatac ttccttcgcc
     9241 ttagcaaata ccaagaccgt ttggtcgaat ttttcaaagc tcatcctgaa tttatcacgc
     9301 cagatggtcg ccttaatgaa atgctacgca acttcatcga gccaggtttg gaagatttgg
     9361 cggtatctcg tacaaccttt acatggggag tgcctgtccc atcaaatcca aaacacgttg
     9421 tctacgtttg gattgatgcc cttcttaact atgcgacagc tcttggctac gctcaagacg
     9481 aacatggtaa ctttgacaag ttctggaatg gaacagtctt ccatatggta ggaaaagata
     9541 tccttcgctt ccactctatc tactggccaa tccttcttat gatgttggat gttaaattac
     9601 ctgatcgttt gattgcccat ggttggtttg tcatgaaaga cggaaaaatg tctaagtcaa
     9661 aagggaatgt cgtttaccct gaaatgttgg tagagcgtta tggactagat ccacttcgtt
     9721 actacctcat gcgtaacctt ccagttggtt cagacggaac ctttactcct gaagactatg
     9781 tcggccgtat caactatgaa ttggctaatg accttgggaa cctccttaac cgtacggttt
     9841 ccatgattaa taagtacttt gatggacaaa tccctgccta tgtagaaggt gtgactgaat
     9901 ttgatcatgt tcttgctgag gttgcagaac aatcaatcgc agacttccat acacacatgg
     9961 aagcagttga ctatccacgt gcgcttgaag cagtctggac tctgatctct cgtaccaata
    10021 aatacatcga tgagactgca ccatgggtct tggccaagga tgaagctctt cgtgaccaat
    10081 tggcaagtgt catgagccac ttggcagcca gcattcgtgt agttgctcac ttgattgaac
    10141 catttatgat ggaaactagt cgtgcagttt tgactcaact tggtttggaa gaagtttcta
    10201 gtcttgaaaa cttgagtttg gctgacttcc cagcagatgt gactgtagtt gccaaaggaa
    10261 cacctatctt tccacgtcta aatatggaag aagaaatcgc ctatatcaag gaacaaatgg
    10321 aaggcaataa accagcagtc gaaaaagaat ggaatccgga cgaagttgag ctcaaactaa
    10381 acaaggatga aatcaagttt gaagactttg acaaggttga aatccgtgtc gcagaagtca
    10441 aagaagtgtc taaagtagaa ggttcagata agttgcttca attccgcttg gatgctggtg
    10501 atggagaaga tcgtcagatt ctttcaggaa ttgcaaaata ctatccaaat gaacaagaat
    10561 tggtcggcaa gaaagttcaa atcattgcta acctcaaacc acgtaaaatg atgaaaaaat
    10621 atgtcagcca gggtatgatt ctctcagctg aacatgatgg caaattaacc cttctcacag
    10681 ttgatccagc tgtaccaaat ggaagtgtga ttgggtaaaa gtagacagga ctagttcagg
    10741 ctagttctgt ttttttattt atactcaatg aaaatcaaag agcaaactag gaagctagcc
    10801 gcaggttgct caaaacacag ttttgaggtt gtagatgaaa ctgacgaagt cagctcaaaa
    10861 cacagttttg aggttgtaga tgaaactgac gaagtcagct caaagcactg ctttgaggtt
    10921 gtagatgaaa ctgacgaagt cagctcaaaa cacagttttg aggttgtaga tgaaactgac
    10981 gaagtcagta accatacata aggtagggcg acgctgacgt ggtttgaaga gattttcgaa
    11041 gagtattaac tattatgctt tacaaactag tgtagaagtg atatactata ggtgaagtta
    11101 ctagtaggta ttacaaaata gattagaagg gaaaagtgat gaaagaaact cagctattaa
    11161 aaggtgttct tgaaggttgt gtcttggata tgattggtca aaaagagcgg tatggttatg
    11221 agttggttca gactttgcga gaggctggat ttgatactat cgttccagga actatttatc
    11281 ctttgttgca aaagttagaa aaaaatcaat ggataagagg cgacatgcgc ccgtcgccag
    11341 atggtccaga tcggaagtat ttttcattaa tgaaagaagg agaagagcgt gtctcagtct
    11401 tttggcaaca atgggacgat ttgagtcaaa aagtagaagg gattaagaat gggggttaaa
    11461 ccatgaagaa aatgaagtat tacgaagaaa caagcgcttt gctacatgag ttttctgagg
    11521 agaatcaaaa gtattttgag gagttgtggg aaagttttaa tcttgctgga tttctctatg
    11581 atgaagacta tctcagagag cagatctatt tgatgatgct agatttctca gaagcagaac
    11641 gagatggcat gagtgcagag gattatctag gtaagaatcc taaaaaaata atgaaagaga
    11701 ttctcaaggg agcacctcgc agttctatca aagagtccct tttgacgcca attcttgtcc
    11761 tggcggtatt acgttattat caactactaa gtgatttttc taaaggtcct ctcttaacag
    11821 tcaatttgct cacattttta gggcaacttc ttatttttct gattggattt ggacttgtgg
    11881 ccacaatttt acgaagaagt ttagtccaag attctcctaa aatgaaaatt ggcacttaca
    11941 ttgttgttgg gactatagtt cttctagttg ttttaggata tgtaggaatg gcaagcttca
    12001 tacaagaagg agccttttat attccggctc cctgggatag tttgtctgtc tttacgattt
    12061 cgctagttat cggtatttgg aattggaaag aagcggtctt tcgtccattt gtcagtatga
    12121 ttattgccca tcttgtggtg ggttctctgc tccgttatta tgagtggatg ggaatttcaa
    12181 atgttttcct tacaaaagtt attcctttag ctgtcctctt tattggcatt tttgtcttgt
    12241 tccgtgggtt taagaagata aaatggagtg aagtatagtc aaaaagccgt tgcaaagcgg
    12301 ttttttgtta taatgaaggg aagaatgtta gaaattaaag gagaaaaatt tgatgagata
    12361 cataactctt ggtcaagatg acaaagaatt atcagaaatt gttctcggaa tgatgagaat
    12421 aaaagataag tctgtaaaag aagttgaaga gcttgtagaa acagcacttt ctgttggaat
    12481 caatgccttc gacttggctg atatttatgg tcgtggtcgt tgtgaagaac tgttaggtct
    12541 tgtcctaaaa aatcgtccag atttaagaga aaagatgtgg attcagtcca aatgtggcat
    12601 tcgcattgaa gaatttacct attttgattt ttctaaggac tatattataa aatcagtaga
    12661 cggtattttg caaagattga agattgatca tctagatagc ttgctccttc atcgaccaga
    12721 tgctttgatg gaatctgacc aagtagcaga agcctttgat ctcctttata aacaaggtaa
    12781 agttcgagat tttggagttt ctaatcaaaa tcctatgatg atggagttgc ttaaaaaaga
    12841 tgtcaagcag ccgttagctg ttaatcagct acaattgagt gcggctttta ctccaggatt
    12901 tgaatcagct tttcatgtta atatggaaga tagtcaagca gctatgcgag atggcagcat
    12961 ttttgaatat tgccaattac acgatgtggt cattcaagca tggtctgtct tgcaattcgg
    13021 gtattttaaa gggaactttg ttggaaatga gaaatttcaa gctttaaatc aagtacttga
    13081 tcgtttagct attaaatatg gagtaacctc ttcaactatt gccatttctt ggatattgcg
    13141 ttatccagca aaaatgcagg cagtcgtagg tacaacaaat cctaagcact tgagagaagt
    13201 tagccgagcg gcaaacttta gcttaacaag aaaagaatgg tatgaaattt atcttgctgc
    13261 agggaataat ttgccgtaag tagtagcagg tttgaataac tcacagtcaa aaagccgtat
    13321 aaagtggttt tttgctataa ttaaatgaag aatgtagaat taaaggagaa aaacatgaca
    13381 tttgaagaga tcctgcctgg gttaaaggct aagagaaaat atgtacgaac tggttgggga
    13441 ggtgcggaaa actatgtcca actctttgat accatcgagc aaaatgggct tgcacttgaa
    13501 atgacacctt atttcctaat caacgtttct ggagaaggag aaggtttttc catgtggagc
    13561 ccgacagttt gtgatgtttt ggcaacggat tgggtagaag tgcatgacta aacgtgtact
    13621 cattacagga gtgagttcag ggatcggatt ggctcaagct cgcctctttt tagagaaggg
    13681 ctatcaagtt tatggagttg accaaggtga aaagccactc ttagagggtg attttcactt
    13741 tttacagaga gatttgacct tggacttgga acctattttt gactggtgcc ctcaggtgga
    13801 tgttttgtgt aatactgccg gagttttgga tgcttacaaa ccactgttgg aacaaacggc
    13861 gcaggacatt caagagattt ttgaaatcaa ctacattatt ccagtagagt tgactcggta
    13921 ttatttgaca caaatgctgg aaaataaaaa gggaatcatc atcaatatgt gttccattgc
    13981 ttctagccta gcaggtggag gtggtcacgc ctatacttca tccaagcatg ccttggctgg
    14041 cttcaccaag cagttggctc tagactatgc tgaagctagg attcaggtct ttggtatcgc
    14101 tccaggagca gtcaagacag ctatgaccgc tgcggatttt gagacaggtg gcttagctga
    14161 ctgggtggct agtgaaaccc caatcaagcg ctggattgaa ccagaggaag tggcagaagt
    14221 cagtctcttt ttggccagtg gaaaggcatc tgccatgcag ggacaaattc tgacaataga
    14281 tggtggctgg tctttgaagt aggaggagtt ggatggaaat caaaaatcac tttggggtct
    14341 actgtgtttg ctttgaaaat ggaaagttac tttgcattga aaaaacgaga ggcccttatc
    14401 aacatcggta tgatctacct ggaggcagtc agcaacttgg tgaaggactg acggaaacgc
    14461 tgactagaga agttatggaa gagacgggat ttactgttag aagctactct aatcctcgaa
    14521 tctacgatgt tttcgtcagg gaagagttaa aaaattttat ggttcaccat gtcatagcct
    14581 tgtatgatgt tgaaatgaat gagagtgcac ctcaagttac gatttcggaa gctgtgtctg
    14641 atggtgcgaa tgattcactt ggatatattt ggatggatat tcaagagatt acagaagaaa
    14701 atgcatcgcc attagtcttg aaggttaagt ctgaattatt aggattgcca gaactggaca
    14761 agacttctta catgaattgg aaggtgaagt agagggataa tggaactatt taaaacatgg
    14821 aagaaaaata tggttctcta tggtcttaaa tctcaaattg gaactgtcta ccgaaacaat
    14881 gataggacaa caagctttta tgatgttggg aattttctat acctagcagg ggagttggat
    14941 tccagatttt gggaagattt tgttagaaaa tatggtttag attataagat tattatttca
    15001 gaaaatacta attggcaaga ttttctgcat cgaaaagtgg ggctaaattc ttttactcgc
    15061 tattctttta aagataaggc aaattttcaa gttgaatttt taaataatct agttactcat
    15121 ttagaggaag gttacaatat tgtgcctatt gataatcata tttataactg cttttctacg
    15181 gaagaatggt cacaggattt acagggggat tttgagtcct atcaggattt tgttttaaaa
    15241 ggtggatttg gctttgtgat tcttaaaaat aatgaactga ttgctgggat ttcctcaggg
    15301 ttagtttacc gtaaagcagt tgaagtggaa gttgcaacta gaccaaacga acaaggaaat
    15361 ggatttgcta aaaagcttgg tgctgcaatg attctagaga gtttaaatag agatatgttt
    15421 ccactttggg atgctcataa tgaggcttcc aaaaaagtag cagaattttt aggatatgag
    15481 ttatctgaac cttatgaagc ttttgaacta gaggaaattt tgatataatt atatatgata
    15541 ttgtatcttt taagacgagg tgaatggcta tgagaaatca acttgccctt agtggcgaaa
    15601 aaattcttga aaaaatttat cctcaactat ttcaccacgt cggcatgatt cgtggggaat
    15661 atttgttgag agagcttaat caaaacatca tattagcaag ttgtcagcaa tttgtaaaag
    15721 attatctaga gactatctgc tccctgtact cagatgaaga agtttggtat cgtttttctg
    15781 aattaacgaa tacagaagcc aattgtttag tggggactag agagtttttt gacgaagggc
    15841 atcccttgtt tggctataga ggaaccaggc gtttactggc gtgtttggat gaatttcagg
    15901 ctgaagcaca tgtcgttaca gaagtttatc aaaccaaccc caatctatct gttatctttc
    15961 cttttgtcaa tgatgccgac caattaaaac aagctattac agtattgcgt cagcagggtt
    16021 ttactgggaa agtcggaacg atgattgaat taccgtcagc gtattttgac ttatctagta
    16081 tactggaaac gggcatttca aagattgtag ttggaatgaa tgatttgact tcctttgttt
    16141 ttgcgactat gagaaacagt caatggcatg atatggaaag tccaataatg ttagatatgc
    16201 taagagatat gcaggataaa gcaagaaaga acaagattga ttttgctgta gcaggctatc
    16261 tgaatacttc tttcatacaa aaaatgaatc agttgggtat caagtgcatt atccactaca
    16321 gttctattcc agagattttt gatttagaga ttgaccatcc agatcatctt aaacacataa
    16381 aagaagaaag taaaaaatta caaaggagta cccatgatac cgcaagaaat gtggaataaa
    16441 tacaagcaaa tcaatccttt gatcggagat gaaatcgatg cttgggcttt tggagttgaa
    16501 ccagatcttt tagcggattt ggtgtttaaa ggagaaaaga cagcaacagc atcagcctat
    16561 gacctctatg tactagaaga cgaaccactt ccccaagtag ggaccttcga tatcatttta
    16621 gatagtcaaa atcagtctgt ctgcattgtc gaaattacaa aggtttctgt tgaactcttc
    16681 aatcaagttt ctgcgcaaca tgcctttaag gaaggtgagg gagacaaatc acttgcctat
    16741 tggcgccagg ttcatgagga ctttttcaca gactgtttgg gtgaagtagg gctgactttt
    16801 acacctgaaa gcaaggttgt tttagaagaa tttcgcaagg tctacccact gtagactatt
    16861 agaaggaaga aagttttgga aatcgctgtc caatcctttt ttttcaagca aaatatgata
    16921 taataagttt gtttgaagaa gagcagcagc tcttaaactt agaataggag aaaactatgc
    16981 aagcagttga acattttatt aagcaatttg ttcctgaaca ttatgattta tttttagatt
    17041 tgagtcgtga gaccaagact ttttctggga aagtgaccat cactggtcaa gcacagagtg
    17101 accgcatctc cctccaccaa aaagacttgg aaatcacctc tgtagaagtt gcaggtcaag
    17161 ctcgtccatt tacagttgac catgacaatg aagcccttca tatcgaattg gctgaggctg
    17221 gtcaagttga attggttctt gccttttctg gtaaaattac agacaacatg acagggattt
    17281 acccttctta ttatacagtt gatggagtca agaaggaggt cttgtctact cagttcgaga
    17341 gccattttgc gcgcgaagct ttcccatgtg tggatgagcc tgaagccaaa gcaacttttg
    17401 acctctctct tcgctttgac caagcagaag gtgaattggc cttgtcaaac atgccagaaa
    17461 tcgatgttga aaaccgtaag gaaacaggta tctggaagtt tgagacaaca cctcgcatgt
    17521 cttcttactt gttggccttt gttgctggtg atttgcaagg ggtgaccgct aaaactaaaa
    17581 atggtaccct ggtaggtgtc tactcaacca aagcacatcc actttcaaat cttgatttct
    17641 cactggatat cgctgttcgc tctatcgagt tttacgaaga ttactatgga gttaagtatc
    17701 caattcctca atctctccac atcgcccttc ctgacttctc agctggtgct atggaaaact
    17761 ggggtcttgt gacctaccgt gaagtttact tggttgtcga tgagaactct acatttgcta
    17821 gccgtcaaca agttgccctt gttgtggccc atgaattggc tcaccaatgg tttgggaacc
    17881 tcgtgactat gaaatggtgg gatgaccttt ggctcaatga aagtttcgct aatatgatgg
    17941 aatacgtctg tgtggatacc atcgaaccaa gctggaatat ctttgaagat ttccaaacag
    18001 gtggagtacc tcttgctctt gaacgtgacg ctactgatgg cgttcagtct gtccacgtcg
    18061 aagttaaaca tccagatgaa atcaatacac tctttgacgg cgctatcgtc tatgccaaag
    18121 gaagccgtct catgcacatg cttcgccgtt ggctaggaga tgctgatttt gctaaaggtt
    18181 tgcatgccta ctttgaaaaa caccaataca gcaacaccat tggtagtgac ctttgggatg
    18241 cccttggtca agcgtcagga cgtgatgtcg cagccttcat ggattcttgg ttggaacagc
    18301 ctggttaccc agttcttact gtcaaagttg aaaatgatgt cttgaagatt tcacaaaaac
    18361 aattcttcat cggtgagaac gaagacaaga accgtctctg ggtggtgccc ctcaatagca
    18421 actggaaagg cttgcctgat acactcgaaa ctgaaagtat cgaaatccct ggctacgcag
    18481 ctcttcttgc tgaaaatgaa ggagctcttc gtctcaacac tgaaaatact gcccactata
    18541 ttaccgacta tcaaggagac ttgttagaag ctgttcttgc tgagctagag acacttgata
    18601 acacaagcaa actgcaaatt gttcaagaac gtcgtttgtt ggctgaggca gggcacattt
    18661 cttatgcaga cttgcttcca gttcttgata aacttgctaa ggaagagtct tatctggtgg
    18721 tttcagctgt ttctcaagtg atttcagccc ttgagcgctt tattgatgaa ggaacggatg
    18781 ctgaaacagc cttcaaagga ctggttgcta aattggctcg tcataactat gaccgtcttg
    18841 gttttgaagc taaagacgga gaatcagatg aggatgaatt ggttcgtcag ctggctgttt
    18901 cgatgatgat tcgctcaaat gatgcagaag ctcgtcaagt cgctagccaa atcttcgcaa
    18961 cacacaagga gaatcttgca gaactcccag cagctattcg ttcacaagtt ctgattaatg
    19021 agatgaaaca tcatgagact aaagacttgt tagcacttta tcttgatact tatactcacg
    19081 caacagatgc tgtctttaaa cgccagttgg cagcagctct tgcatacagt acagatgcgg
    19141 ataatatcca aaacttgatt acttcttgga aggacaaatt tgtggtcaaa ccacaggact
    19201 tgtctgcttg gtattaccaa ttcctagctc atcaagcaac tcagaaaaca gcttggtctt
    19261 gggcgcgtga aaactgggct tggattaagg cagcccttgg aggagatatg agctttgata
    19321 gctttgttat ccttcctgct catgtattta agactcagca acgcttggcg gaatacaagg
    19381 agttctttga gccgcaactt tctgaccttg ctcttagccg taacatcggt atgggaatca
    19441 aggaaattgc agcacgtgtt gacttgatta gccgtgaaaa agctgcagtc gaagcagttg
    19501 ttcttcaata cggaaatgca taaataagcc taaaataaaa agaaaaggct ctttgtcaac
    19561 tgtagtgggt tgaaaaaaag ctaagctcga gaaaggacaa attttgtcct ttcttttttg
    19621 atattcagag cgataaaaat ccgttttttg aagttttcaa agtttcga
//
LOCUS       DAVKLX010000039        19196 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKLX010000039 DAVKLX010000000
VERSION     DAVKLX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19196
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.039"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            31..960
                     /locus_tag="VW325_001533"
     CDS             31..960
                     /locus_tag="VW325_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001150386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9734902.1"
                     /translation="MQDESIYGLVYKTIREERGFTQKEAAGTTVTPHFLRQFEQGKSR
                     ITIQKFEEILSNIGIDKETFDSLKTQMFPTEFHKLQAQVADLAYKREFKKIISLINQP
                     MENPGIAEHYVIANRVVNKFAIANIIGDSFLTPKDYQELEYIKNYLSELDDWNKIEVN
                     IFSSILPHFSIEFLDYRLYHLLDTLKKQTEYHSIRTADYYIACLRTAIKHYSVNGYYD
                     KAESLAVKTLEVINTFPLLSTKMTEMISLSMERANNFLRKDDVRGLELAKHIFASLDN
                     FEKVYPNQLLTRMREDFFVTVTQLNHTGQPLDV"
     gene            complement(1010..3346)
                     /locus_tag="VW325_001534"
     CDS             complement(1010..3346)
                     /locus_tag="VW325_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9734903.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLGPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGAALNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESRVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQVDQD
                     NDVNRIIEQLEEQTLESHKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKILQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQVQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDAFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(3450..3998)
                     /locus_tag="VW325_001535"
     CDS             complement(3450..3998)
                     /locus_tag="VW325_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9734904.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(3995..4297)
                     /gene="zapA"
                     /locus_tag="VW325_001536"
     CDS             complement(3995..4297)
                     /gene="zapA"
                     /locus_tag="VW325_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9734905.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            4384..5265
                     /locus_tag="VW325_001537"
     CDS             4384..5265
                     /locus_tag="VW325_001537"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9734906.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVITRDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            5269..5883
                     /gene="lepB"
                     /locus_tag="VW325_001538"
     CDS             5269..5883
                     /gene="lepB"
                     /locus_tag="VW325_001538"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9734907.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            6011..8377
                     /locus_tag="VW325_001539"
     CDS             6011..8377
                     /locus_tag="VW325_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9734908.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(8425..9708)
                     /locus_tag="VW325_001540"
     CDS             complement(8425..9708)
                     /locus_tag="VW325_001540"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9734909.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(9902..10261)
                     /locus_tag="VW325_001541"
     CDS             complement(9902..10261)
                     /locus_tag="VW325_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9734910.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNRFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPIIAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNIT"
     gene            complement(10560..11189)
                     /locus_tag="VW325_001542"
     CDS             complement(10560..11189)
                     /locus_tag="VW325_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9734911.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVCYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGFPTSQKNLTGITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLSFIYSVLKGYSLEGW"
     gene            complement(11234..11473)
                     /locus_tag="VW325_001543"
     CDS             complement(11234..11473)
                     /locus_tag="VW325_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734912.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(11623..11889)
                     /locus_tag="VW325_001544"
                     /pseudo
     CDS             complement(11623..11889)
                     /locus_tag="VW325_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(11914..12225)
                     /locus_tag="VW325_001545"
     CDS             complement(11914..12225)
                     /locus_tag="VW325_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002876249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9734913.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKTMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(12150..12392)
                     /locus_tag="VW325_001546"
                     /pseudo
     CDS             complement(12150..12392)
                     /locus_tag="VW325_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(12393..13025)
                     /locus_tag="VW325_001547"
     CDS             complement(12393..13025)
                     /locus_tag="VW325_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9734914.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQDEF"
     gene            complement(13039..14034)
                     /locus_tag="VW325_001548"
     CDS             complement(13039..14034)
                     /locus_tag="VW325_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9734915.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(14031..14729)
                     /locus_tag="VW325_001549"
     CDS             complement(14031..14729)
                     /locus_tag="VW325_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9734916.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(14806..15816)
                     /locus_tag="VW325_001550"
     CDS             complement(14806..15816)
                     /locus_tag="VW325_001550"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9734917.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(15800..16807)
                     /locus_tag="VW325_001551"
     CDS             complement(15800..16807)
                     /locus_tag="VW325_001551"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9734918.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRSFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTETLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(16794..17747)
                     /gene="mvaD"
                     /locus_tag="VW325_001552"
     CDS             complement(16794..17747)
                     /gene="mvaD"
                     /locus_tag="VW325_001552"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9734919.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(17729..18607)
                     /gene="mvk"
                     /locus_tag="VW325_001553"
     CDS             complement(17729..18607)
                     /gene="mvk"
                     /locus_tag="VW325_001553"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9734920.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(18729..19058)
                     /locus_tag="VW325_001554"
     CDS             complement(18729..19058)
                     /locus_tag="VW325_001554"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9734921.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TSCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         5564 a         4347 c         3626 g         5659 t
ORIGIN      
        1 aatagaaaaa aggattcgag gagatttagg atgcaagatg aaagtattta tggattagtc
       61 tataagacca tccgtgagga aagagggttt acacagaagg aagctgctgg gactacagtg
      121 actcctcatt ttttacgtca gtttgagcaa gggaaatctc gaataaccat tcaaaaattt
      181 gaagagattc tttctaatat tggaattgat aaagagacct ttgatagtct caaaacacag
      241 atgtttccaa cggaatttca taaattacag gctcaagttg ctgatttggc ctataagcgt
      301 gaatttaaaa agatcattag tttgattaat caacccatgg aaaatcctgg gatcgctgaa
      361 cactatgtga ttgccaatcg tgtagtcaat aaatttgcta tcgcaaacat catcggcgat
      421 tcttttctga ctccaaaaga ttaccaagaa ctagaatata tcaaaaacta tctgagtgaa
      481 ttagacgact ggaacaaaat tgaggtcaat atcttttcat ctatcctgcc tcacttttca
      541 atcgaatttt tagattatag gctctaccac ttattagata ctctaaaaaa acagactgaa
      601 taccattcaa tccgtactgc tgactactat attgcctgct taagaactgc tatcaagcat
      661 tactctgtta atggctacta tgataaagct gagagcttag ctgtgaaaac cttggaagtg
      721 attaatactt tccctctact gagcaccaag atgacagaga tgattagtct ttccatggaa
      781 cgggctaata attttctcag aaaagatgat gtcagaggtc tagagcttgc taaacatatc
      841 tttgccagtc ttgataactt tgaaaaggtc tatccaaatc aacttctgac tcgaatgaga
      901 gaggatttct ttgttactgt cacacaactc aatcacactg gacaaccttt agatgtctaa
      961 cctagaaaat aaaaaagctt gaggaaccac atatttcctc aggcttttct tatcctttaa
     1021 aagtgacaat agtcgcgcca ctgcctccag cattttgtgg ggcatagcca aaactcttga
     1081 catgtttgtt tctttgtagg tatttggtga ctccttcacg gatgacacct gttccgatac
     1141 catgaatgat atcaacttga gccatattgt taagcagggc ttggtcgata aaggcatcta
     1201 gctcattcat ggcctcttca tagcgtttgc ctcgaaggtc cagtctggct tgtggacctc
     1261 ggccagatgt ccgtttaaca acattgactt gtttcttctt aactggcttt tcttgctgaa
     1321 cttgaacaag gtcaaactct ttctcttcca gggtcatctt aatcaagcca acttgggctt
     1381 cccagcggcc gtccttgagt tgactggtca gggtaccacg ctggccataa ctgagaacca
     1441 cgatatcatc tcccaccttt ggagctcgtt ttttcttggc cttttgaagg atcttgtttt
     1501 tagacaaatc tactttttca ggagccaatt ttttcagctt ggccttggct tcaatgattt
     1561 cgtggggctt gagttgagat ttactgtgga gatttttgag aatctggtca ctctcactga
     1621 gggccatgtc cacaatctca gcagcctgtt cacgcgcctt attaagctcg gtttcctttt
     1681 cacgattgag ttcgttgtaa agttttttga gagcacggtt catcttgaga ttttcttgct
     1741 ccacctcacg gatattgtcc aagcgtttat gactttcaag cgtctgctct tccaattgtt
     1801 caataatccg attgacatca ttgtcctgat cgacctgctg gctggcatcc cctacgataa
     1861 cttcagatag gcctagacgt ttggcaattt caaaggcatt acttcggcca ggaacaccct
     1921 gcataaagcg ataagtcggg cgaagagttg cagtatcaaa ctccatacta gcattttgca
     1981 caaaggctgt ctcaataccg taagccttta gttccggata gtgggtagtc gccatggtct
     2041 tgatttgacg caggcgaagg tcctccagaa tagccatggc aagagcagct ccttcttgag
     2101 ggtctgtacc agcccccaac tcatccaaga gtaagagtga atgttgattg accttgccaa
     2161 gaatatccac gatattggtc atgtggctag agaaggtaga caagctctgc tcaatagact
     2221 gctcatctcc aatatcggca aagatttctt caaagatacc aacacgactg cccttatctg
     2281 ctaaaatcgg caaaccggac tgagccatga cctgtgtcaa gccgagagtt ttaagcatga
     2341 tggtcttacc acctgtattg ggacctgtaa tgacaatagc tgttaaatct tgaccaaaat
     2401 ggacatcatt tgcgacagca tttttgacca aaggatggca gacatggagc agttgaatct
     2461 cttgattttc tgacagctga ggtacgactg cttgcctttc ttggataaag cggaccttgg
     2521 cacgaatcaa gtctagatga ccgataatcc aggcgtcatt ggcaatctca gccgcatgag
     2581 ggcggacacg ctcagaaatt tcttggagaa tgcgaagcat ttcatagcgc tcatctgctc
     2641 gcagactagc aatttcttcg ctcagtttga ctacctcacg tggttcgata tagacggtgt
     2701 ttccactagc agaaatatca tgaacgacac ctgcaatctt attgcggtag gtgtttttga
     2761 ctggtaaaac ctgacggcca tttctgctag caacaattcc ttctgtcaac atctgcgctt
     2821 tttgcttgag caagtcttgt aaaacatcgc gtacccgact ctcgctatca tgtatttttc
     2881 gacggattcg cgccaattct tcactggcaa aattttcaat gaaacccgca tcattaaagg
     2941 cctgaagatt tccttgtaat tgcggaaaat catgtaattt ctcaaaccaa agggctaatt
     3001 cttccaagct gacattttcc agattggcgt aaaaactttg aagttctcgg ctggcaagaa
     3061 gcacgcgttt caagagtagg aactcctcga tattgagagc cgctcccatc tccaacctct
     3121 tgcagactcc tgcaatttcc ttagttgaga gaatagtaaa atgcggttgc tcgacgaaaa
     3181 gagcctgcat ttccttcatc tcagcaaaag cctgtttgat tttatctgct ttggcagtcg
     3241 gacccagctg tctcaattgc tccaagccct gctcggtcaa caaatgaggc tcaaacaagg
     3301 ccttgacctt atcgaactct aatgtttcta atattttctt attcatcttt atttccttgt
     3361 ctttgaatgt ctaggtgtgg tagcttttta cattctgata gattcgagtc aaatctgtaa
     3421 acaaagggct aggaaaactc ctgccctttt tatccgatta aatttgtcac ccagatttgt
     3481 ttgagccaac tggttgttat cggtatgctc tggatgatgt gttttgcgac gatactcttt
     3541 tcaagaggat tttgtataac tgccatgggg atggtcgcca agattgtcaa ggccatttgc
     3601 aagacaaata aggtcaccaa catggacaag atacctgctg aaacttggaa caacttacca
     3661 cccagttttt tgctaggaat caagtgtaag agaagaccaa gcaaacgacc aatgctatag
     3721 acaatcccaa atacaagcaa gtagccgatg cctgcataaa agaccttatc cagctgaaag
     3781 agttgatccg atgggaaaaa gaaagtcccc tgaccttcct gcgaatttgc ataagggagc
     3841 aataaatgga attgctctcc aagcccctta taaaactggc cagccataaa agccgatgcc
     3901 atggctgaaa tcaggtaata aacctgtaag agcaggcctc tccgatagcc gatataaaat
     3961 ccccaaacca agaccaatag aagaaggaat gaaatcataa ggaatcctca atcttgctct
     4021 gttcttgctt acaagtcaca agcttgtgac ggagttcttc tagctcttgc tccttatcgt
     4081 caaattcaat ctcacggctg agctgagttg ataaacagtt gactgccaac aaaagagcga
     4141 ttgtttcatc atctgcgcta ggcatttgtt ctttaattgc ttggtatttt tctgtcgcaa
     4201 ccttagcgat ttcctccata aaaaggttat catgttcgct tgtcaaggtt aacgattttt
     4261 tcccgaatgt aaatttgaat cgatttagat ttgccataaa attcacctca cgatattata
     4321 ccaaaattcg ctaattttgt cagtttttac aaattttact gcttttgtgg tacaatagaa
     4381 actatggcaa gtatcacact cacaccaagc gaaaaggata ttcaggcttt tcttgaacac
     4441 tatcaaacca gtctggctcc tagcaagaat ccctatatcc gctacttttt gaaactacct
     4501 caagcaacgg tttctatcta tacttctgga aaaatcttgc ttcagggtga aggggctgaa
     4561 aaatacgcca gtttctttgg ctatcaagct gtagagcaaa ccagcggaca aaatcttcct
     4621 ttaattggga cagatgaggt gggaaatggt tcctactttg gtgggcttgc agttgtggct
     4681 gcctttgtca cacctgacca gcacgacttt ttacgaaaac tcggtgtggg ggattctaag
     4741 actctgaccg accaaaagat ccgtcagatt gctcctattc tcaaggaaaa aatccagcac
     4801 caggcactcc ttctctcacc cagcaagtac aacgaggtca tcggagaccg ctacaatgct
     4861 gtttcggtta aggttgccct ccataatcag gctatctatc tcctccttca aaaaggtgtt
     4921 cagcctgaga aaattgtgat tgatgccttt accagtgcta aaaattatga caagtacttg
     4981 gcacaagagg ccaatcgttt cagcaatcct atcagcttag aagaaaaggc tgagggcaaa
     5041 tacttggctg tcgcagtttc ttctgtcatt acgcgtgatc tctttctgga aaatcttgaa
     5101 aatttgggac gagaactggg ttatcagctt ccaagtggag ctggaacggc ttctgacaag
     5161 gtggctagcc agattttgca agcctatggt atgcagggac tcagcttctg cgccaaattg
     5221 cactttaaaa acactgaaaa agcgaaaaaa cgcttagaaa ggtaagttat gaattcattt
     5281 aaaaatttct taaaagagtg gggattgttc ctcctaattc tgtcattact agctttaagt
     5341 cgtatctttt tttggagcaa tgttcgcgta gaaggacatt ccatggatcc gaccctagcg
     5401 gatggcgaaa ttctcttcgt tgtaaaacac cttcctattg accgttttga tatcgtggtg
     5461 gcccatgagg aagatgggaa taaggacatc gtcaagcgcg tgattggaat gcctggcgac
     5521 accattcgtt acgaaaatga taaactctac atcaatgaca aagaaacgga cgagccttat
     5581 ctagcagact atatcaaacg cttcaaggat gacaaactcc aaagcactta ctcaggcaag
     5641 ggctttgaag gaaataaagg aactttcttt agaagtatcg ctcaaaaagc tcaagccttc
     5701 acagttgatg tcaactacaa caccaacttt agctttactg ttccagaagg agaatacctt
     5761 ctcctcggag atgaccgctt ggtttcgagc gacagccgcc acgtaggtac cttcaaagca
     5821 aaagatatca caggggaagc taaattccgc ttatggccaa tcacccgtat cggaacattt
     5881 taagaaacct aagaggccga gaatcaccaa tctcagcctc ttcttctatc gtgagaaaat
     5941 gattggtaac tatctaaact taccagaaca gaaacacctc aactctcacc tattcatgca
     6001 aaggaatttt atggaagttt atttttcagg aaccattgaa cggattattt ttgaaaatcc
     6061 cagcaatttt tatcgcatcc tcctcctaga aatcgacgat acggacgcag aggattttga
     6121 tgactttgaa atcattgtca caggaaccat ggctgatgta attgagggcg aagactatac
     6181 tttttggggg caaattgtcc agcactccaa gtatggagaa caactgcaaa tcagtcgtta
     6241 tgatcgcgca aaaccaacta gtaagggctt ggtcaagtac ttttcaagta gccatttcaa
     6301 ggggattggt ctcaagacag ctcagaaaat cgtggatacc tatggcgaaa ataccattga
     6361 cgaaattttg caacacccag aaaagttaga aggcatcgca ggactctctg ccaaaaatcg
     6421 cgaggctttc gtctccactc tccgtctcaa ctacggaacg gagatggttt tggccaaact
     6481 agccaactac ggcattccca acaaactagc ctttcagatt caagactttt acaaggaaga
     6541 aacccttgat gtggttgaaa attatcccta ccagctggtt gaggatatca agggtttggg
     6601 ctttaccatt gctgaccaac tagctgagga actaggcatc gaaagtcagg ctcctgaacg
     6661 cttccgcgcc ggtctagttc acagtctttt tcaggcctgt atggaaacag gggacaccta
     6721 tgttgaagca cgggatttgc tggaacaaac ccttactctc cttgagtctt cccgtcccgt
     6781 ggaactagac cccagccaag ttgcccaaga gctctcctac ctgatcgaag aagacaaggt
     6841 tcagcagatt gataccaaga tctttgacaa cagcctcttt ttcgctgagg aaggcatccg
     6901 cagtcacttg gttcgtatcc ttgaaaaagg aaaacagaag agtcatgatt taaaaaccat
     6961 tcaaaaacat atcactacta tcgagcaaga gcttggaatt gagtatgata acattcaaaa
     7021 acaggctatc tgtgatgcta tccagaataa ggtctttatc ctgacaggtg gacctggtac
     7081 tggtaagaca actgttatca atgggatcat cgctgtttac gccctgttag agggacttga
     7141 cttcaggaag aaaagcaacc tgcccattct tcttgctgct ccaactggtc gagctgctcg
     7201 tcgcatgaat gaattgacag gtttgcctag cgcgaccata caccgccact tgggaatgac
     7261 aggtgacgat gataccagtc atctggaaga ttacctagat gctgacttta tcatcgtgga
     7321 tgaattctct atggtggata cttggctggc caatcaactc ttctccaata tctcttctaa
     7381 cagtaagatc ctcatcgtag gtgacagtga tcagctacct tctgtcagtc ctggacaggt
     7441 tctagcggat ctgcttcata ttcctttgat tcctcagact cgcttggaaa aaatttatcg
     7501 acaaagcgaa gaatcaacca tcgtcaccct agctagtcag attcgacagg gcatcttgcc
     7561 agctgatttc acccaaaaaa aagctgaccg ttcctacttt gaaattgcta gtggccatat
     7621 tcctgccacc attgaaaaaa tcttaggtgc cgccctcaga aatggtattc ctgcccgtga
     7681 tatccaagtt ctggctccta tgtaccgagg gacggcaggg attgatgcta tcaatcagct
     7741 catgcaagac ctgctcaatc ccccacaaaa agatcaactc agttttgaag ctcctcagtg
     7801 ccactatcgt aagggagaca aggtcattca tttggttaac gatgctgaaa tcaatgtctt
     7861 taatggggat ttaggagcta tcacagacct gattcctggt aaatacaccg aatcgaaaca
     7921 agacgagatt gtcattgatt ttgatggtaa tgaagtctct tacccccgta acgaatggta
     7981 caagattcgc ttggcctatg ccatgagtat tcataagtct cagggaagtg agttcccagt
     8041 tgtcatccta ccgattacta gtgctagccg acgtatgctg gagcgaaatc tcatctacac
     8101 agccattaca cgtgccaaaa gcaaactcat cttactaggc gaattacaag ccttcgacta
     8161 tgctacccaa cacatcggaa ctgcccgaaa aacctatctg attgaacgct tcagtgatct
     8221 actggagaat gttgaagaaa agcaacaagc tgtctctgaa acagtcacat caagtgcctc
     8281 tgaacaatcc tacatcctaa ccgaagaaaa ctgggacagc attccagcca tgattgggat
     8341 tacagacaca gacctcaaag agatttttgg aaaatagtac atcagaaaac ccaccaattc
     8401 aggtgggttt tctgtttatt aagattattt tactgttgct gtgcttgtga tcaattcaac
     8461 agcttttttg atagtgatat catgtttcaa catgtcagct gaaagcaagt tttgaacttg
     8521 tgcaacttcc atgttgtagt ctgctgccaa ttgctcaact tctttttgga tttcttcttc
     8581 tgaagcatca aatccttcag ctttggcaac tgcttcgata acaaggttag tcttagtacg
     8641 tgactcagct tctgcttggt attggttgtg aaggtcttct tgagtagttc cagtgatttg
     8701 gaagtacatg tcagggttga tcccttgacg ttgcaagttc ccaaggaatt catttactga
     8761 acggtgaact tcttcatgga tcatttcttc tggaagttct acgatttcag cgttttctac
     8821 agctgtatca attgctgcac cttcaactgc atctttgtaa gcttcttctt tagcagcagc
     8881 caattctttg cggtattttt ctttcaagtc agcaagtgtt tcaacttctt catcaatgtc
     8941 ttttgcaagt tcatcgtcaa gagctggaac ttctttagct tttacttcgt ggatagttgt
     9001 cacgaattta gcttctttac ctgcaaggtc ttctgcttgg tagtcttctg ggaatgttac
     9061 gataacatca acggtttcgc cagctgagtg acctaccaat tggtcttcga aaccagggat
     9121 gaattgacct gaaccaagtc caagtgagaa gttttcacct tttccaccgt caaattcaac
     9181 accgtcgata gaaccaacga agtcgatcac aacagtgtcg ccgttttcag cagcagcttc
     9241 cttgataacc aattcagcca gattgttgcg ttcgcgttcg atacgctctt caacatcagc
     9301 gtcagttact tctttttcta catcaactga tacttcaagg tttttgtagt cacccaattt
     9361 tacttcaggt tttgtaacga cttcagcagc gataacccag tcttgacctt tttccattga
     9421 agttacgtca atttttggtt gggcaaccac ttcaagacca gcttctttta cagctgcttc
     9481 ataagcgttt ggcaaaagag cgttcataac gtcttggtaa agtgattctt caccaaattt
     9541 tttatcgaag ataggacgtg gaaggtgacc tttacggaaa cctggaacat taagagattt
     9601 cttcactgac ttgaagacac ggtccaattc tggtttgatt tggtcttgag agatagtgaa
     9661 agtcaagaca ccacggtttg tttctttgtt ttcaaatgat acagacattc tgtcatttct
     9721 ccttaaaatt ttttaaatac agtctattat aacattttct ataaataaaa tctagactaa
     9781 tgcagacaat tatagacaac aagccttttt cctaactaaa acactcataa agtatagaca
     9841 attttagact actttggata tcttttggtt agtcattttc caaaactaat ttatctaatt
     9901 tttaagttat atttgacttc atcttctcag taacatgagt ataaacactt aaaatctttg
     9961 tttcattagt ataatctaca cgatccatta tcgctattat aggtacttcc aattcagcaa
    10021 gcaaacttat atgcgaatat ctaaaaacat gagcagtcaa tacaaaatca tctttcttaa
    10081 tttcactttt cctaaacctg tttttcaaaa agtaattgaa acaagttatc attaagggag
    10141 aaccattatt tgtaaatata taatcacctt cttctaaaag aagatattct tttaaaattt
    10201 ctaacactct atcactaata ccaattactc tatatgaagc taaacttttc ggactatcca
    10261 aaaacacttc gttattggac gattacatta tagtaaaacg aaataagaac aggacaaatc
    10321 gatcaggaca gtcaaatcaa tttctaacaa tgttttagaa gtagaggtgt actattatag
    10381 tttcaatcta ctatacaaga cgaaattgaa gaaatctttc cattataaaa cgcatattat
    10441 caaggttttt aaacacctaa taatatgcgt tttttcctat tttaaagact tttatccacc
    10501 tattatacaa agttaagtat ccatcacagt ttacaattag ccttcattac ctaacccatt
    10561 caccatcctt caagagagta acctttaaga accgaataaa taaatgacaa atttcgttca
    10621 tttaatttaa ctgcactgtt aatttgatta gctccatctc ctaaagtatg cactcctact
    10681 acacggtgac tagcatcata aactgtagat ccactagacc cctctaaagt atcaacttgg
    10741 taatccaaga acatgccatc atcacttaaa acttgttttt tatctgtata catttgatga
    10801 attttaaaat tatatgatgg atagcctgtg atagtcacag ttattcctgt caaatttttt
    10861 tgactagtag gaaaacccaa agtccctaat tttgcaccaa tgggctcttc tagaattaat
    10921 aaagccaagt catattccct tgcatcctta gaatttaaat ttctaaattc cttcaaataa
    10981 caaacttcct ttactttgat ctttccaaat agttcttgac ttggactaac agccggaaga
    11041 acataaatat catccgcttc tttcccatag tcatgtctgt agtagttgtg agccgctgta
    11101 attaacacat tatctttaat aaaattcgca cttcccctcc cttctacatt cattccatcg
    11161 gctactccat aatacttaga taaaaccata gctgaagtcg aatacggata ctgtaaagta
    11221 ttatcaattt taatcaaatc atcattaccg ataatacttc tgattgcttt tggtagtatg
    11281 aaccatacgt tggtgaaatc tcagataatg aagaatcatt agaccctgga cctttttcta
    11341 gtgtctcact tacctcatat tcttcacctg tactagaaat aacactcaaa gcagatactg
    11401 tcgataactg gctagccaat aaagtactcg caataattga aatacccaat tttttataaa
    11461 cagttttctt cattattgta tcctcctaat gtaattatag cgtactattc taaatttctt
    11521 aatctactat agaatcaaga aatctaccac cttctttaaa taccctccat tatcacataa
    11581 acaggtaaac ttttcaatta atgactgcgc ttttcaatca cgctagaggt acttacttgc
    11641 ttctttgata ctaagttcag ccattctttc cttgtttttc tcaataaagc atgttaccca
    11701 agtgggattc gttttggagt agtctcgcag agtccagcca atggctttat tgataaaaaa
    11761 ttctgtttgg ttcaagttat gaagtagaat cttttccatt aattgagtat tggtcttctc
    11821 ttttcttaac aactggtggt caatagcgac acgtctcagc cagatattat ctgataggct
    11881 ccattttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca gattgctcaa
    11941 aacactgttt tgaggttgca gatagagctg acgtggtttg aagagatttt cgaagagtat
    12001 taagattatt tcttctagtt cagggtgttc atacaccaaa ctccctacta ctcgatctag
    12061 gatatctacc gtgtcccacc aggattttgt cacgactaac tgctctagct taggcaaatc
    12121 ggtttccttt agataagact gcattgtttt caaatagtta gcagccacat attggtattt
    12181 tctaggctcc ttttcccagc aagtgtctgc aaaatcccaa tcgataatct ttgttttttt
    12241 cgcttctagt aaatattttt atagagttta tttctttcag gcaccgcaat acctagaaaa
    12301 gaaaattgat ggcgcatata ggcttccatg gaccttgctt ttttagagtc ttttgctgct
    12361 tctagctcct caagtaaatc tgctaaactc atctaaaact catcttgccc caccaaatgg
    12421 tgctgaaagg catagacagc cgcctgggta cgatcgctga cttcaagttt ggcaagaatg
    12481 ttggacacgt gggtcttgac cgtcttgaga gagataaaga ggtcatctgc gatgcgctga
    12541 ttttcgtagc ccttggcgat gagttggaga acatctcgct cacgcgcagt caattcttca
    12601 tgaagttcca tatgattgcg gtggtattca accttcttgc taacctcttg ctcaatggcc
    12661 agctcgccag cagctacctt actgacggca tgaagcaatt catctgcact agaagtcttg
    12721 agcatatagc ctttggcacc agcatctaag actggcatga ttttttcatt gtccaaataa
    12781 gaggtcacaa tcaaaatctt ggcttcaggc cattctttaa ggattgctaa ggtcgcgtca
    12841 atcccattca tctcaggcat gacaatatcc atgacaatga catctggacg cagttccaag
    12901 gccaagtcaa tcccttgaga cccgttggac gcctcaccca caacttctac atcgtcttgg
    12961 aggtcaaagt agcttttcaa acccaaacgg accatttcat ggtcatctac tagtaaaatt
    13021 ttcatcttta ctcctttatc attccttatc taacagggga atacggatat caactgccag
    13081 cccttgcttg ggagctgtca agagttgaac tgttccagcc atatcttcaa ccctctcctt
    13141 gatatttcgc agtccataac tcaagtcgtc taagcttcct aaccggaaac caatcccatt
    13201 gtccaccacc ttcagttgca attcaacatc tgtctgatag aggtagacat ctaggcaaga
    13261 tgcctgggca tggcggaggg tattgctaat caactcttgc aggatacgga agatatgctc
    13321 ctcgattttc ttaggcaatt tcgtcatatt ctgcttgaga ctaaccctaa gatcactctt
    13381 gtcctcaagc tcttttaaga gaatttgaat cccttctatc aagctcttct gctccagttc
    13441 aactggtcgc aaatgcaaga gcaaaacccg caaatccttc tgggcagttt ctaaaatagc
    13501 tgtgacactc tgcaactgga tctgcatctt ttctctatcc aatttcaaag cctgctgact
    13561 gatacccgat aaaatcatgt gggccgcaaa caactcctga ctgactgtat cgtgcaaatc
    13621 ccgagcaatt cgcttccgtt ctttctcgat gatttcctct tcctgagcaa ggctatgatt
    13681 ttcagctttt tgaagagctt ctgtcaaaag gttaagttta cctgataagg acttgaaact
    13741 ggcatccaaa tctggatctg caacctgaac cacttcttgc cctgccaata aacgcttgag
    13801 attagcctgc atttttctta gagaaagctc ttcgatccct cgccaaaaca gggctaagag
    13861 acaggttatg gacatgctga aaaccaacaa taaaaagaca aatttttctg ttttttcgac
    13921 atcgtgcaaa aagatagacc agtcaaaatc aagtatttcc agcaagctgt gggagaaaaa
    13981 aaagacaaac aggaaggagg tgagagcaat aatgacatag gcttgttttt tcatcctcta
    14041 accacctcca catcaccaat catagtggtc aagaaaatct tgacactctt gttactcttg
    14101 agatagtctt ttgtttcttg atgatagtgt tcattgcgga gggctcgctt gggctggttg
    14161 aaaaaagtca aatccccata gagacagtta acgctgagac tgacttccac atctacaggt
    14221 acgatgattt tggtcgttcc taccatcttt ctgaggataa tgacattgtc atgattggtt
    14281 aagatgaccc tctccagatg aatagtgtcc ttgcccatga agcgaaagag attgatatca
    14341 tcgaattggc aagtctggta gcttgaaaaa tgatgaagat ttccaaacca acgatttttc
    14401 tccttcttaa ccgtcacgac ctcttcaaaa accaaattgg tctgctcttt ttcctggttc
    14461 atcatcgggt aaagaagaaa gaggctatag ataaccgcaa caaaaatagc tagaatcaca
    14521 aaaggattga gcataacgat gaaaaagaag agaatggttg ccgctactaa aagaagatta
    14581 tttccctctt taccagtgta gtagcgaatc aaaagcaaaa agaggaatag tatcagcaga
    14641 aaacgcgaaa aatgctctga taccatcaaa atcagagctc ctgtcagaag acaggcttcg
    14701 ataaataaaa agattttaaa ttttctcata ggttcatcct ctcccttcta ttttatcaca
    14761 attcaaaaaa gtcacctcag tctgaggatg gaaaaaaggc gctggttacg cctttttcat
    14821 ctgatccttt gcttctttta attttccata aagaagatag tctacttttt gtagatctgc
    14881 tatggtggca cagttaaggg aacacataat caagcgtagg tctgctttcc agccttggac
    14941 aatgccaatc acttcttcaa ctgtgtaggt ttcaaccaat tccagaacgg ttcgtgacaa
    15001 tcccacagac ttagcaccaa aaaccaagca cttaatcata tccagcggat tccgaacccc
    15061 tccactaacc aagagttcga ccttatcttt ccattcttgg gcattgagaa gggcctgcat
    15121 ggtagactga ccccattgat tgaggtaatc acgctggcca ctacgacggt tttcgatata
    15181 ggcaaagctg gtgccaccac gacccgatag gtccactgta cgaacaccga attcataggc
    15241 tctttcgatt gtcttggcat ccattccaaa gcccacttcc ttgaggacaa taggaacggg
    15301 aatttgcttg ctataatctg ctagatgcga ttgccagctt ctaaacttcc tttctccctc
    15361 gggcatgagt aattcctgca tgacattgac atgcacttgc aatagaacag gattcatctc
    15421 ttctacagtc tgaagtccta actcgacagg tttgtccaat ccaatattgg ttccaaggag
    15481 gagattggga tgactagact tgacagaaaa agaatcatcc gttggatttt tgagggctgc
    15541 gctataagaa cccgttacaa ataaaatacc acaggattcc gccacctgag ccagcttttg
    15601 attgatttct cttcccttat tacttccacc agtcatggca ttgatataaa aaggaaagtc
    15661 ccactttcga ccagcaaact ctgtcgaaag atcgatttca tccagattgt aaagaggcaa
    15721 ggaagaatga atcagctcca cctcatcaaa gctattatag gaacttttct gctcaagggc
    15781 atagaggata tgctcgtcct tacgatttgt cgtcatgtcc tatcctttct tgatataaga
    15841 gctcaatccc cagatcggcc caacgatttt ttaaggtttc ggttgattgc gcatcaaaac
    15901 tcaaggcgat gccacagtca ccaccaccag caccactact cttggcaacg gcctgcaaat
    15961 cttgactggc ttctttcaac tgtctaagca aaggcgtgta aatatctgta ctcaagcctt
    16021 ctaaaagctt gctggctact tctacttgct cgataacttt ttcggctttc ccctgctcca
    16081 aggcttcgac caaagaaacc accgtttctt ttgaggaagt taaaaaattt tgattgatat
    16141 tttgcttgat ttgctggacc atgtgactcg atacagccac ttccttggtc catcccacta
    16201 agaaatcaca ttctaaagtt ggtttcactt gtgagataaa aaatccccaa tcacgctcca
    16261 gaactgtcgc caagttttct tcttctaacc aagcagccac cttctggcga tcaaatgact
    16321 ggtagagaac caaatcctct gccacaatac aggcaaggtc gcccatggaa ccattgtctc
    16381 ctcgcttgag caagacagcg ctagtcagct tgaacaagag attctgatca accgaaagat
    16441 tatagagagc cagtaaagcc ttgacaacca agacaacaac gctgccacta gaacctagac
    16501 caaacttttt cccttctcgt tccattttgc cacagatttt tagggaaaaa gatcttaaat
    16561 tctgaccgcg aacagcgagg aagtctccca tcaaagcaat cgtttcttga atcaagctgt
    16621 agtcaggatt aggccttaag tccactgcga aatcaaacat atctgaatag atacggtagc
    16681 tgtcagaaaa agcaatttca gccctcatat agatgggaat atcctttatc aaagctaact
    16741 gccctggctc taaaatagca tattcacctg cccaatagag ttttccgcaa gttttaacag
    16801 caatcatctt gactcaaatc ctttgttttt gacacaatca agcgataacg ctgaccgaaa
    16861 atttctgata aatgctccaa gtctttctcc tgacagaaga ccttaacatt gggaccagca
    16921 tccatggtaa agtagcaggc ctctcctttc tcacgaagct ggcgaacaaa gtccatagcc
    16981 tcataagagg catccgtcag ataagaaaag gctggactag cagtctttgt cgtagcatgc
    17041 atagccaggg catttttctc cgttaattct ccaatcttgg caaaatcatt ttccttgaga
    17101 taaatcagca tatcctgata gtccttctca gactgacgaa cccagtcgtc gaaagtcgtc
    17161 gaggtttcca cacaaagttt catcccgtca cggctagaga ttggtttttt cttgtcctct
    17221 agcaccaaca taatcatagc tagtttcaag tctgtctcta cagggtaaat ttctccacta
    17281 tccttatccc aggctcctag tggtccataa aaactccgag aagaagagcc tgaggcaaat
    17341 ttggcttcct gtgccaactg acttctatcc aatccaagtt tgaaataagc attacaagcc
    17401 ttgaccaggg cggacaaacc actagaactt gaggacaggc ccgctgccgt aggcatattg
    17461 ttttgagtat cgatacggac aaagccctca ccagctggac gataacggtc aataatctta
    17521 ctcatcttgg catgctcgac ctcattttgt agctgaccat tgatgtaaaa ttcgtcagct
    17581 gttacattgg ctggtaaagg cgacaaggtc gtctctgtat acatattttc caaagttaga
    17641 gaaatactgc tagtagcagg caccatctct ttttcttttt tctttcccca atatttgata
    17701 atagcaatat ttgcgtagga acgtactgtt acaggctctc tatccatgtc tgaacagctc
    17761 ctttctcttc taatctttct gctagttctt gtgcgtgtgt caaattggtt accaaggcta
    17821 tgatacaacc tcctagccca ccaccgctca tcttggcacc cagagcacca tggctaagag
    17881 tcgtttcaac caaaaagtct gcctcagggc tactgactcc aatttctttt aaatgtaaat
    17941 gcgcttgact gaggatttgt cccagtcctt cagcatcttt ttgtgaaatc gcaacttctg
    18001 cttgctgggt taattctccc aaggcatgca aaaacggtag ggcatccttg cccttatttt
    18061 gaaccacttg gatggcttca cgagtatgac cataaacacc cgtatcggca atcaccaaat
    18121 aggcggataa atccatctca agttctgtaa atcctacgtt cttgataaag cgaataggtt
    18181 ggtcactgag acaggtctta gcatccaaac cactaggatt catatgggca atcatttcag
    18241 ctcgattgac caagatttct agtacatcat gaggcagatc agcctgatag tagtcaaata
    18301 ccgcacgaat ggccgctatg ctgatagccg ctgacgaacc catcccccgt ttctcaggga
    18361 tagccgagtc aatctcacaa cgaatgcagg cttctgtgat atccaaatac tccagtgagg
    18421 cataaaccgc catggacaag gtatcctcct cataaaggcg ccaaggactc tctgcaggaa
    18481 ctaccttaca ggtcacctcc acctccaaaa gaggcaggga aatggcagga taaccgtaaa
    18541 cgaccgcatg ttcccctatt aaaattatct tactatgtgc ctgaccgaca ccaacttttt
    18601 ttgtcatttt ttccttttac tagacgaaaa aacgtcttat ttttcataca agtattaatt
    18661 ctttcctatc tattttatta tattttcaca aaaaaagcga ttgtttccat tcacaatcgc
    18721 ttctttcatt attgaaccca ttcgccatta tagttgacag aatagccatc tacggtcgta
    18781 ttcactgcca aggcacctga gctataagcg tagtaccatc tgccattgac ctggaaccaa
    18841 cctgtcgtca tagaacgacg aaagaaactc cataccatta agtaaagagg aaagtcgtga
    18901 gggagcatgc gccattgaca acttgtttta gtgacgtaca aagtctcatt aacaagtact
    18961 cgtttcggcc atttataggt gcggtgtttg gagaaatagg gttcaatctt agcccattct
    19021 tgatcgttta aatcagtatc atatgctttg cgtgtcataa ctctagttta acattttttt
    19081 gtgaatacag gttctaaata atcgaccacg aaaatttctt aagtggaaaa cgccttatga
    19141 agtatgctac gggaaagtta tgcacttaat ttgacaattc aagatgtaaa aatata
//
LOCUS       DAVKLX010000040        18512 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKLX010000040 DAVKLX010000000
VERSION     DAVKLX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18512
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.040"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(177..1592)
                     /locus_tag="VW325_001555"
     CDS             complement(177..1592)
                     /locus_tag="VW325_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9734922.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1605..3443)
                     /locus_tag="VW325_001556"
     CDS             complement(1605..3443)
                     /locus_tag="VW325_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9734923.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3461..4300)
                     /locus_tag="VW325_001557"
     CDS             complement(3461..4300)
                     /locus_tag="VW325_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9734924.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(4600..7158)
                     /locus_tag="VW325_001558"
     CDS             complement(4600..7158)
                     /locus_tag="VW325_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9734925.1"
                     /translation="MERSQFEEMFQLQSSRLTTQEKLQLFTSVFAGRYDVYAKNFINE
                     QGKIQYFPSYDYGWKQLPPEKRSFQTLTNSVLKSHFRGEAAIGIFPMHLDDSCYFLVL
                     DLDEGDWKEAGLTIRRIARERQMEAHLEISRSGYGLHIWFFFEEAILSRKARLFGKKL
                     LELAMQESMQLSFDSFDRMFPNQDVLPKGGFDNLISLPFQGEAYHQGRTVFVDEQFQP
                     YEDQWRYLQEIQKISTAKVALLIQEELGKQELDKELKIVLSNMIQLEKSSVTPKTLFF
                     LKNMASFSNPEFYLKQAMRQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVSV
                     EDRRKVQRSISVEFKGELTFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKT
                     KTIILVHNKQLLDQWLDRLNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKL
                     VDVVMIQSLFKLENSQSLLDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPE
                     RKNGHEPIVFQRIGEILHTADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWI
                     ATDSARNQLILKDILAQVAEGRNILVLVNRIQQIDVFEKLLKEKEVDGCYIISGKTKV
                     RERTSLLETLEQLDKGFVLLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIH
                     RNYKDKSLVRIFDYVDIHVPYLEKMFQKRQVAYRKMDYRVIEGEEKQFVYVDSRYEKV
                     LREDLAGERQECLLILPYVHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKI
                     KVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTILRLESASIVSELVAGLR"
     gene            7706..8906
                     /locus_tag="VW325_001559"
                     /pseudo
     CDS             7706..8906
                     /locus_tag="VW325_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9021..9824)
                     /locus_tag="VW325_001560"
     CDS             complement(9021..9824)
                     /locus_tag="VW325_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9734926.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(10107..10862)
                     /locus_tag="VW325_001561"
     CDS             complement(10107..10862)
                     /locus_tag="VW325_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEU9734927.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCFSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(10932..13583)
                     /locus_tag="VW325_001562"
     CDS             complement(10932..13583)
                     /locus_tag="VW325_001562"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9734928.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(13595..14557)
                     /locus_tag="VW325_001563"
     CDS             complement(13595..14557)
                     /locus_tag="VW325_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEU9734929.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDTNFAYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKLFLKLNHNTLVDIKSKLESLKLKHSRYYISK
                     ISKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN"
     gene            complement(14559..15116)
                     /locus_tag="VW325_001564"
     CDS             complement(14559..15116)
                     /locus_tag="VW325_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9734930.1"
                     /translation="MARTELPDQIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNCCDSLIYGLLKSEWEE"
     gene            complement(15103..15552)
                     /locus_tag="VW325_001565"
     CDS             complement(15103..15552)
                     /locus_tag="VW325_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU9734931.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(15965..16360)
                     /locus_tag="VW325_001566"
     CDS             complement(15965..16360)
                     /locus_tag="VW325_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9734932.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(16456..16710)
                     /locus_tag="VW325_001567"
     CDS             complement(16456..16710)
                     /locus_tag="VW325_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9734933.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(16720..18054)
                     /locus_tag="VW325_001568"
     CDS             complement(16720..18054)
                     /locus_tag="VW325_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9734934.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTIAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(18054..18287)
                     /locus_tag="VW325_001569"
     CDS             complement(18054..18287)
                     /locus_tag="VW325_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9734935.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            18318..18395
                     /locus_tag="VW325_001570"
                     /pseudo
     CDS             18318..18395
                     /locus_tag="VW325_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
BASE COUNT         5693 a         3992 c         3051 g         5776 t
ORIGIN      
        1 ataaccaatc tatcctaagt taactattta taatcggctc tctgtaacaa aattaaatta
       61 attaatcccc ctataaaata aagaggttta gaatttgatg tcttccaaac ctctttattt
      121 catatttaat gagatgctgc cttaaccgtg ataatatcta atcatcaata aacaaactat
      181 ttgaataagg attctccatt tgattcaatc actcctttat accaagtaaa ggacattttc
      241 ttatatcgat tgaatgtgcc atttccatca tcatttcgat caacataaat gagaccgtac
      301 ctcttagaaa gctgtgcagt ggacatagaa acgcagtcaa tacatcccca agacgtatag
      361 cccataattt ccacaccatc ttgtagagct tcagctactt gcaataaatg ttctttcata
      421 tactgaattc tataatcatc ttggactgtt aagttattaa attcatcttt tattagttga
      481 tctttagcac ctaatccgtt ttccactata aataatggaa tttgataacg gtcataatat
      541 ctatttaaaa ttatacgtag tccaattgga tcaatttgcc atccccactc agaagactct
      601 aaataaggat ttactaaacc accaataata ttcccttctc ctgaattata ctgtgttgga
      661 agagcagatt gagtcacact catgtaatag ctaaaggata aaaaatctac agtataattt
      721 tttaataact ctgcatcttc agctgcaaac tctatgttaa tgtcattttc cttaaaatat
      781 ctttttgcat aattcggata ataacctcta acatgcacat ctgaaaatag ataatttaga
      841 ttctcatact catgagtcgc ccatacatct tttggatttg gagtcattgg ataagctggc
      901 atagctaata ccatacaccc caccttaaac tctgaattaa tctcacgagc aatttttgta
      961 accaaactcg aggcgactaa ttcatgatgt atagcttgat ataattcttg tttcgaaaga
     1021 ttctccttag gtatatctat tcctccacta gtaaacggta attccaaaac agagtttact
     1081 tcgttaaatg taagccaata tttaacttta tctttatacc tttctaaaac tgttcgagca
     1141 aatttttcat aaaaatgaat cattctccta tcaatccatc catgatattt tcttgctaaa
     1201 tataatggag tctcatagtg tgaaagagtt acaagtggtt ctatcccgtg agcatgtagt
     1261 tcatcaaaca attcatcata atatttcaac ccagcttcgt taggttcttc ctcatctcct
     1321 tttggaaaaa ttctactcca tgcaatagaa gtacgaaaaa cattaaagcc catttcagaa
     1381 aacaaggata tatcttcctt atatttatga taaaaatcaa tacctatcaa ttttaagtta
     1441 tcttctgtag gattttctgt tgcttctcct aatccacctt tgggtaacac atcctgaact
     1501 gataagccct taccatcttc attatatgct ccctctactt gattagctgc aacagctcca
     1561 ccccaaagaa aatcatctgg aaaaatggtc ataactttcc tccattataa tattaccagt
     1621 aattccttag aatgctcgat tgtctgatta ttaggtaata ctaatacatc tagaaaatca
     1681 ttggtattcg ttacaattac tggtgtaact gtttcgtagc ctttagtctt gattaaattc
     1741 aagtccattt caaaaatcaa ctgatttttg aaaactctgt ctccttcttc tacatgacta
     1801 ataaaacctt gaccttttag ctcaacagta tctaatccaa tatgaattag taactcaaca
     1861 ccctcatcac tcttcaatcc aattgcgtgc ttagtcggaa aaatatttgt aattttccca
     1921 tcaaatggtg cataaacctt accttcactt ggaataatcg ctactccgtc tccaattagt
     1981 ttatctgaaa atgttttatc ctggacatcg cttaacggaa tgatttctcc tgatatagga
     2041 gaaaatatca tttttttatt tgaaactcca gctttaactt ctaaattgct agaactctct
     2101 tcttcatcga ttccaaatat ataagctaat acaaaggtaa taacaaccga aataaccgcc
     2161 acaattaaag catttacaat atttgatggc acatcagaat aaataaattg aggcaacgct
     2221 atcaaagatg ggacagcaaa tagatacgct ttaacactag taagacctgc aaataatccc
     2281 gctaatccac caccaatcat agctgcataa agcggttttt tatattttaa agtcacacca
     2341 tataatgcag gttcggtaat ccctgcaagt aaggctgaga aacctgctgc aaaagcaatt
     2401 tgttttgtat tattattttt actctttaat gcaacagcca tcgaagcagc cccttgagct
     2461 aagtttgacc ctaacattgc tggaagaatt aatacgtctg gagtagcaat agatgccgcc
     2521 aaaaaaatag gtgcaaaagc ccaatgcatt ccagtcataa caataaatgg cataatagca
     2581 ccaagaatag ctaatgtaag ccatccagct acaccataca tttgcccaac tagatttgat
     2641 aatccttcac caacaattac tccaataggc ccgactacaa ctaaggcaat acagcttgat
     2701 actaataata ctagcgtagg ttgcaaaaaa ctcttagtaa cagctggtgt taatttagca
     2761 atcatttttt caatatattt catcaaccaa accataataa gaattggaac gactgacgaa
     2821 ccataactag ctggtgtcac aggtacacca aataaactaa gaggattccc tgattgcacc
     2881 atttgaacaa aatttggatg gagaagtaca cctgctacag acatagctaa tgtagatgtt
     2941 acttttaatt tttgtgatgc agaataagct aataacagcg gtaagaaata atatggagca
     3001 tccccaaaaa atgtcaaaaa agcaatagtc tgagaatctg attgcaatat accaagcatt
     3061 ggtaaaataa ttaccaagac tttcagcata cctcccccta acattgctgg aatgattgga
     3121 gtcatggaac cagcgatata ctcaatgatt ctttctaaaa tattcccttt gtgcccttga
     3181 acaactgaat cggattcaaa attgccaagt ttaacgaatt ctttataata attagctaca
     3241 tcattaccaa gtataatttg atattgtcca ttctttttca taatacctat tacacctggt
     3301 atcttcttca catcatcatc attgactaaa ttttcatctt ttaattctaa tcttaaacgt
     3361 gttacacaat gggtaactct attgacattt ttttcacctc caattacatc gaggattttt
     3421 tgtaccgtat ctttataact catggtattc tcctattcta ttaatctaaa ttttttgtta
     3481 agcgacgaat atgagccatc aaataaacta attcactaga agtcagcaaa taattgtact
     3541 ccgtttgtat aaacattgcc acctgttcac cacattcata ttctctagga tatttatttt
     3601 tcattaatgc taacaagtct tcatcatcat cgtcacaata agttgtattt tcaaccaagc
     3661 gatgggcaaa taattttata tgagttatga atctataata atcaatagaa tcttggttaa
     3721 actcagtaca atagaaatct tgaatgattt tctcaattcc cataattatt tcagtgattt
     3781 tatacgactc ttgaaaatta ttttctaaat tagcattaac aaaatgtagc gctatgaatg
     3841 cagcttcgtc aattggaagt tcaataccta actctttttt tattatttgt aaagcccttt
     3901 tcccaagttc aaattcttct ttataatatt tcgatatttc ccagcgtagg ggattcgaaa
     3961 taataatccc ttctttataa cgttctatgc tcgaagaaat atgatccgtt aaattaatat
     4021 agataatttc atcaaaatta ttacccaatg ttattttacc taggttaatt attttttcgg
     4081 tgatgtacac caactccaaa ggaacgttaa taaacatctc tgtaaaacgg gtcatattat
     4141 ctctattttt gagaaaaaaa cttttctcaa tatcaattgg atttacttta tctccagttt
     4201 ttcttccaaa agctattccc cttccaaaaa gaagaatatc gatatctttt ttactttgcg
     4261 caattacggc attgtggttt agtattcgtt ttatgatcat aaaatttccc ttttaaaata
     4321 aaaggaacct acttaaaaac atcaagaaac ttcttctgat gtgattaaat aggttcctgc
     4381 tcacttgtat tagtgataac atccttttat ttcaattttt aatggtgaca tgaagcttaa
     4441 ataattaata actctgtaac cgtttttatt ttatgatatt tcaatctatt tgtcaagtac
     4501 ttataaaaat ttttttattt tgtgtgattc gaatgtgata atggcaacag tctcactaaa
     4561 gaaatgaaaa tcatacatag aaatttttaa aaattttctc tatcgtaaac ctgccactag
     4621 ttcagaaact atactagctg attccaaacg taatatggtc atctcatcta ccttccctaa
     4681 taatggcatt gcaccatacc aaacaatagt cttattcacc aaaagaatag gcgttactat
     4741 ttttgattga gtaaaagaca ctttaatttt ctggctcttc aactggtcta gccatgcttt
     4801 atttgcaacc gtctctggta tacatatctc aatttgacta atcctaaatt cttttagaaa
     4861 attcatcagt tttgtctggt gcacataagg taaaataagc agacattcct gtctttcccc
     4921 tgctaagtcc tctctcaaca ccttctcata tctactatca acataaacga attgtttctc
     4981 ctcaccctcg atgacacgat aatccatctt tcgataagct acttgtcgtt tctgaaacat
     5041 cttttctaaa taaggaacat gaatatccac ataatcgaaa atacgcacca aagacttatc
     5101 cttgtagttt ctatgaatcc gacctgcata ctgaattaaa ttatttttcc aagaaaaggg
     5161 tgctgccaag ataagcgtgt ccaactgagg taagtcaaaa ccttcgccaa tgtattttcc
     5221 agtagacaac aaaacaaacc ctttatctaa ctgttctaac gtctccagta aactcgttct
     5281 ttctcggact ttggtttttc cgctaataat gtaacagcca tcaacctctt tctctttcaa
     5341 taacttttca aagacatcta tctgttgaat tcgattaact aaaaccaaga tattccgtcc
     5401 ttctgccact tgggctagaa tatccttgag aatcaactga ttcctcgctg agtctgtagc
     5461 aatccaatca ctgagctgta taaaattact tgctttggtc ttttcaatct ccaaatgacc
     5521 aaaagaagtg aatcttaatt gcaattgccg tttaaaatcc gtttccctct tatcagcagt
     5581 atggagtatc tcaccaattc tctgaaaaac aataggctca tgaccattct tacgctcagg
     5641 cgtagccgtc aaaccgtaaa gatacttccc tctaaactga gcaacaactt tttcaaacat
     5701 caaggcagag acatgatgac actcatccac aatcatcatc tcatactcat ccaaaagact
     5761 ttgactattt tccaacttaa atagagattg aatcataacg acatcaacca gtttactcag
     5821 ccatttctta gtcccaccgt actgtccaac atagccgatt accttttcac gacctgatgc
     5881 cgtataacgg atagcctcct cctcttcgaa agtcaaaaag cagtttaagc gatctagcca
     5941 ttggtctaag agttgcttat tatggactag aataattgtt tttgttttcc gttcagagat
     6001 aagagcagca cctaaaacgg tcttcccaaa accagtctcc gcatgaagta aaccattttc
     6061 tttagaagtc atatctgaca gggctaactc ttgctcaaaa gtgagttctc ccttaaattc
     6121 cacgctaata gacctttgta cctttctcct atcttccaca gatacctgct taaatttatc
     6181 ttgcaatgga tacagcaaac ctcttggcaa ccataaataa tgatcggatt ctccaaataa
     6241 atacattctc tcaggaattt gataggttgg ctgtcgcata gcctgcttta aataaaattc
     6301 gggattagaa aatgaagcca tatttttcaa gaaaaacagt gtcttgggtg tcacagacga
     6361 tttttcaagt tggatcatat tggataagac gatcttcaac tccttgtcca attcttgctt
     6421 gcctaactcc tcttggatta acagtgccac tttagcagtt gaaatcttct gaatttcttg
     6481 tagatacctc cattgatctt cataaggctg aaactgttca tccacaaaga ccgttcgccc
     6541 ttgatgataa gcttctcctt gaaaaggaag ggaaattaaa ttatcaaatc cacctttagg
     6601 aagaacatcc tgatttggaa acatgcgatc aaaagaatca aaggacagtt gcatactttc
     6661 ctgcatagcc aattctaata gtttctttcc aaacaggcga gcctttcgac tcagaatcgc
     6721 ttcctcaaag aagaaccaaa tatggagtcc gtaacccgaa cgagaaatct ctaaatgggc
     6781 ttccatctgg cgttccctgg ctattcttcg aatggttaaa ccagcttctt tccaatctcc
     6841 ttcatccaaa tccagtacca aaaaataaca gctatcatct aagtgcatag gaaagatacc
     6901 gatagctgcc tccccacgaa aatgagattt caaaacggag ttcgtcaatg tctggaaact
     6961 ccgtttttca ggtggcaact gcttccaacc ataatcatag gaaggaaaat actgaatttt
     7021 cccttgttca ttgataaaat tcttagcata aacatcataa cggccagcaa acacagaggt
     7081 aaacaattgt aatttttctt gcgtcgtcaa gcgactactt tgaagttgga acatctcctc
     7141 gaactgagag cgttccataa caaactgact cttgtcttta agacgtttat agtggaatga
     7201 aataagatat gaacaaattg attaggaaaa tcaaattaat ttatagaaat cttttagcag
     7261 tcatggcgtc ctattccaga ttcaaaatgc tatacacaag aaaaactcac tatccccatt
     7321 aaaagaagaa aagacatcaa ggacttctac cacaattcca tccaaagaca caaaaacagc
     7381 cataagagtc acctccttga ttcctatagg ctgattataa caagactggc tgaaattgta
     7441 catgaaaata aaatcctaat agtactcatt ttgtatgtga ctaatattcc gtctcgctcc
     7501 agaaggtacg aagtaaatag agtttaaacg agcagtctat aaaattcaaa aaacgcatat
     7561 tatcaagcga cctaaaactt tgggctctat aatatttgta gtgggtaaat tccctatgga
     7621 tattatggag cctattttgg gtagaaaaaa agtcccataa gatctataat gaaaagcgac
     7681 aaaacaactc attagaaaga atcatatgga acaattacat tttatcacaa aattactaga
     7741 cattaaagac cctataaaat tccttacctt gaaacgactg gtatgcctac tagaattctc
     7801 cttagaaaac gtcgttttaa gtgctatcat tgcttgaaaa tgatggtcgc tgaaacttct
     7861 atcgtcaaga aaaatcacca aattcctcgt attatcaacc aaaagattgc gcaaaagtta
     7921 attgaaaaga tttctatgac cgatattgcc catcagctgg ccatttcaac ttcaactgtc
     7981 attcgaaagc tcaatgactt ccactttgag tgtaatttta gaaatctgcc taagattatg
     8041 tcttgggacg ttgaaacagt ccggggagtg actgtttcaa tcgggagatg gagatgagct
     8101 ttattgcgca agattttgaa aagctcgata tcatcactgt tcttgagggt agaacacaag
     8161 ctgtcatccg agatcacttt cttaaatatg atagagccgt ccgatgtcgc gtcaaaatta
     8221 ttactatgga tatgtttagt ccttactatg acttagctaa acagcttcgc tttcaaattt
     8281 ctaggctcag gctgaaacag tctcccagac tgtttcactc ccgaatgcta aaatcgttct
     8341 tgatcgcttt catattgtac aacatcttag ccgtgctatg agtcgtgtgc gtgtccaaat
     8401 catgaatcag tttcatcgaa aatcccatga atacaaggct atcaagcgct actggaaact
     8461 cattcaacag gatagccgta aactcagcga taaacatttt tatcgcccta cttttcgtat
     8521 gcatttaacc aataaagaga ttttagacaa gcttttgagc tattcacaag acttgaaaca
     8581 tcactatcag ctctatcaac tcttgctgtt tcactttcag aataaggaac cggagaaatt
     8641 tttcgaactt atcgaggaca atcttaagca ggttcatcct atttttcaga ctgtctttaa
     8701 aaccttcctc aaagataaag aaaaggttat caacgccctt caactacact attctaatgc
     8761 caaactggaa gcgaccaata atctcatcaa acttatcaag cgcaatgcct ttggttttcg
     8821 aaactttgaa aacttcaaaa aaacggattt ttatcgctct gaacatcaaa aaagaaagga
     8881 cgaaatttgt cctttctcga acttagcttt tcttcaaccc actacaattg acaaagagcc
     8941 aaactttgat gatatacgtt ttcttgtgag aatatttact tcattttttc ctaaaattca
     9001 atgtttactc agtatttgga ttatgaaaaa tcgaggtcta aatctagata cattttttct
     9061 gaagacaaat cattttgacc accgagcaag agattttcaa aaaaagctgt taaaaactca
     9121 ggacgtcgct gtaaaatctt tgcattatct aataccaagg catcacgaaa atacttggaa
     9181 tgttgctgaa atggtatatt atcaatatca aaaccaaact cacgaagata ctgaatcaaa
     9241 aagaccgtta ctgtccgagt gtttccttcg cgaaatggat gaatctgcca gattcctgaa
     9301 ataaaatgct ggatttgttt aaccacatcc gcctgagtta gtgtcgcata tgcaacttgt
     9361 ttttcctgat taaaatcata atctaaggtc atttgaatca tggagtaatc agagtacaca
     9421 acactttcac cattcaaaac aggttcattc tttgtgatat tggtctgacg aaattgcccc
     9481 accggaatag agggttcaaa tatatcttga aacaactcct tatgaatagc aagtaaggtc
     9541 gcaggactaa agctaaagcc tcttcgagac aatagttcta caatacgtag agaaaccaag
     9601 tctgcctctt ccgtacttgc atcaatagta tggtgataag ccgttgcatc ctcataaacc
     9661 tgctcataag tcagttctcc ccgggactgt ttctcagcca aagattccat atacgctgat
     9721 ggcactagat tgtcaacttt ctgcagacca aaacctatcc gccataaatc acgcttcgct
     9781 tcataagaca agtttggatt gtcaatgttg taagttggtt gcatagaaat atcctttcaa
     9841 ttgtttttct tcattaccag taaatcacga aattggttgg aaactggctt aaactataaa
     9901 aagtcctcac aataggttct atgatatata tatatatata tacttcatgt aaataataaa
     9961 agagttataa tcagcactac tgataaattc aataccgatt tcgccatctt aaaacttagt
    10021 caaacgaata ccgttgattt ccagttcatt aagtcgttga cctgtcaaga cttctcgttc
    10081 cttcaactgg aaaagttaag ggggaactaa tacaaatcct ctacgcttct atattcaatg
    10141 atttcatttt tgataatatt ctttttaaac tcaaaatgtt ttaaaggatt atcgattaac
    10201 actaactttg gaatatcatc tagatttgtg accaaggata aaataaaatc cgattgaaac
    10261 tccttgactt tttcaaattc ttttgcagtt aaacgcacac gccccttaga ttttcggata
    10321 ccttttactt cggcaaggta agaatactct tggacatcta cctgaaagtc atatccatca
    10381 ccataaagac gggcatcggt taactgtcca ccttggaatt tttcttcttt atcaaaatga
    10441 aggataaaat aattctcagc ctccattccc gtttcctgta gacgtttgaa tttagttcga
    10501 actattggtt tttcaacgat ggttatgcct gttttcttcc cttcactggc aagtaacatt
    10561 ttaactattt cagcataact atgaacatct tcatttccaa acatcatatc aattaaatct
    10621 ttacgaaaac gataaacttc agctttctgc caccaacctt ttcgattatt gtcaaaataa
    10681 ggatcaaata aatccattct attttttaca actccagttg tttcagcaat tcccagagaa
    10741 accacatagc gataaaactc cgacttgcta aagcattgaa attcttttat aaaaagcttg
    10801 tcaaacttgg caagaccgta gccaattaaa tttaacaatt cataatgagg gtgtttggac
    10861 atcactttct ccatcaatag atattgattt cattatacca aaaaacacgg ctcatcaccg
    10921 tgtttctgtg tttatttcac caacttcttc atctcatcaa tacgtgctac ggtcacatca
    10981 tacttggctt ggtagtcggc ttgtttgtct ttttcttttt ggacaacttc tggtttagcg
    11041 ttggcgacga agcgttcgtt agagagtttc ttaccaacca tgtccagttc tttttgccat
    11101 ttagcgagtt ctttgtcgag acgagctagt tcttcttcaa cattgaggag atcggccagt
    11161 ggcaagaaga tttcggctcc tgtaataacg cttgacatag ccagttcagg tgcagggatg
    11221 gttgatgcga tttccaagtg ttctggattt gtaaagcgtt tgatatagtt gacattgctg
    11281 ttaaagaagg cttccaagtc actatcgctt gtcttaacaa ggatggtgat aggcttgctt
    11341 ggtgctacat ttacttccgc acgcgcattc cgaacagcac gaattaagtc tttgagactt
    11401 tccacaccag tgtgagccgc aaggtcttca aaggctagat taacagttgg gtatgcagct
    11461 gtcacgatag aaccttctga gatttgtcca aagatttcct ctgtcacgaa tggcatgatt
    11521 gggtgaagga gacgaaggat cttgtccaaa gtgtatagta gaacagaacg tgtgatgact
    11581 ttctcttctt cgttatcgct ataaaggact tccttggtca actcaacata ccagttggca
    11641 aattcttccc aaatgaagtt gtaaaggata tgaccagcca caccaaactc gaacttatca
    11701 aagttttcag taacttttgc aatggtttcg ttgagattgt ggagaatcca gcggtccgtc
    11761 acattaccag cctcacctgt tgcaactttt gtgacattgt catgcgccac atccagcgtc
    11821 aaacctccat tgttcatgag gatatagcga gaaatgttcc aaatcttgtt aataaagttc
    11881 catgaagcat ccattttctc ataagagaaa cgcacgtctt gtcctggcgc agaaccgttt
    11941 gaaaggaacc atcgaagggc atcggcaccg tatttctcga taacatccat tgggtcaatc
    12001 ccgttaccga gagacttaga catcttgcgt ccttgctcgt cacgaatgag accgtggata
    12061 aggacgtttt ggaatggttg acggcctgtg aattccaatg actggaagat catacgagac
    12121 acccagaaga agatgatgtc gtaacctgtt accaaggttg aagttgggaa ataacgttta
    12181 aagtcttctg agtcgacttc aggccagccc atggttgaaa atggccagag ggcagaactg
    12241 aaccaagtat ccaagacgtc ttcgtcctga gtccatccgt caccttctgg agcttcttcg
    12301 ccgacataca tttcaccatc agcattgtac caggcaggga tttggtgacc ccaccaaagc
    12361 tgacgagaga taacccagtc gtggacattt tccatccatt gaaggaaggt atcgttgaaa
    12421 cgaggtgggt agaattcgac cttgtcctct gtgtcttggt tggcaatggc gttcttagcc
    12481 aattggtcca tcttgacgaa ccattgagta gacaagcgtg gctcaactac gacacctgtc
    12541 cgttctgagt gaccgactga atggacacgt ttttcgattt tgacaagggc accgatttct
    12601 tccaacttag caacgactgc cttacgagct tcaaaacgat ccatgcctga aaattcaaag
    12661 gcaagctcat tcatagttcc gtcgtcgttc atgacgttga cttgtggcaa gttatgacgt
    12721 tggccaacca agaagtcatt tggatcgtgg gcaggtgtga ttttcacgac accagtacca
    12781 aactcaggat ctgcgtgctc atctccaacg attgggatga gtttattagc gattggaagg
    12841 atgacgtttt taccaatcaa gtccttgtag cgcgggtctt ctggattaac cgcaaccgca
    12901 acgtccccaa acatagtctc aggacgagtt gtagcaactt caagggcgcg tgaaccatct
    12961 tccagcatgt aattcatgtg gtagaaggca ccttctacat ccttgtgaat cacctcaata
    13021 tcagaaaggg ctgtgcgagc tgctgggtcc cagttgatga taaactcacc acgatagatc
    13081 cagcctttct tgtaaaggtc cacaaagacc ttacgaacag cttttgacaa accttcgtca
    13141 agagtgaaac gctcacgaga atagtctaca gaaagcccca tcttgcccca ttgttccttg
    13201 atggtagtgg catattcgtc tttccattcc cagaccttcg tcaagaaaga ctcacgacct
    13261 aggtcataac gcgtaatacc ctcaccacgt aagcgctcct caaccttagc ctgagtcgca
    13321 ataccagcgt ggtccatacc tggaagccaa agggtatcaa agccctgcat gcgtttttga
    13381 cggatgatga tatcctgcaa agtcgtatcc caagcgtgac caaggtgaag tttcccagtt
    13441 acgtttggtg gtggaatcac gattgaataa ggcttagcct tttgatcgcc tgaaggcttg
    13501 aaaacatccg catcaagcca tttttggtaa cgaccagcct caacctcggc tggattgtat
    13561 ttagatgaaa gttctttaga catgtgtgtt ctccttaatt acttttattg acaattttaa
    13621 ccaaaccaat ttttgaaagt tcaatataaa tctcttcaat ttcagcaaat gctgcaagtg
    13681 gtgacatagt ataatttcca ggattatatt taagatcata cttgcttatc tttgaaatat
    13741 aatacctaga gtgtttcaat tttaatgatt ccaactttga tttaatatct actaatgtat
    13801 tatggtttaa ctttaaaaac aacttattat aattaccata gagctctgta agagcattaa
    13861 agtattcttt cactagagct atagcgtcaa tcttagatgt ttcttggttt agttttatca
    13921 actccccttt caattttttc catttaaagt tggaatttaa aagataatct aaatcaatat
    13981 agaaatctat tataacagtc tcatcatctg ggctaacagc ttttacctca tttattggta
    14041 agtcagtatg ttgcgaataa tttctcaaag aatggcaaaa tcgataagca aaattggtat
    14101 catactgata gcttgcaaaa ccatgtatac aatcaaactc ctcactatct ttggaatatt
    14161 tttgtttaat ttggttttcg ttaaaatcga caaacaattt tccactggat aataaatgaa
    14221 tcatgaaacg atttacatct gcctcttcaa tttctaattc ctttgactca agtttatttt
    14281 taatacttaa aaaatccgag acatctgaaa taaacaattc gaacatattc ttgatttttt
    14341 gaaaattctt ttgaatctca aaaatttggt ccgagactga cattatttcc ttggttggtt
    14401 caatatcaga gatgacaata cgtttttcat tgttgttaat ttctaaataa gctaatcgct
    14461 cgataatatc actatcattt aattcagcat actcaacatc aatttggcgt aactgcttct
    14521 cagatagaga gcttacttcc tcttttacat cgctcatctt attcctccca ctcgctcttc
    14581 agcaagccat atatcagact gtcacagcag ttgccttgag catctttgcg gtctcttatg
    14641 cgagcttcga gggtaaagcc aagcttttcc gcgactcgtt tactttgaag gttatatcca
    14701 aagcaagtta gttcaatctt gtgaagaccc aaatctttaa aggctaagtc aatcaaggca
    14761 cgcgcagctt ctggcacata acctcgcccc caatagtctg ggtgtaaggt atagccgatt
    14821 tccagcacat catcttcatg gcgatggttg aaatcgacag agccaacgat cttatccgtt
    14881 cctttgacga caatcccgta gcctgctggg agattttcct tttgattacg ctccggaaga
    14941 atgtgctcca gataataaat ctcatcttcc aaggtcttga ctggaggaaa acctgctgga
    15001 taggcgacct ctggcaaact agcatagtca aagatatcct cagcatccgc cacagtacgg
    15061 actcgtaaaa cgagacgttc tgtttcgatt tgatctggca gctcagttct tgccatcctt
    15121 cttcctcgct tttttgatga aactgccctt catatctaca cgcttatcca gatagcgata
    15181 aacgcgctga tatccatctc ccatgaaata ggttggggca aacagttgat ttttaaaatg
    15241 tcccttttca tccaggagtt ctggggcaac aagtcgctca agaatcttgg caaagatgtg
    15301 gcaaataccg tcttcctcaa caatcctatc taccctacaa tctaaaacaa gtggacaggc
    15361 gtctaaaata ggagtctgag ttcgttcaga aatttcataa tgcactccca aacgttccaa
    15421 tttctcctga tgactgataa aaccagcctg ctccatcgca agcatagaag tttcatcaga
    15481 aatattcaca gtaaattttt gatactgttt gatctgctct gcggcattct ctctcgcaac
    15541 gactccaatc acaacccaat ctcctagact ataagaggaa ctacaggtcg tgatgttata
    15601 gccaaaattc taatcttgat atcctaaaat aaaaacagga aaaccataat atagtttact
    15661 tgtgttaaaa gattgcttca taacaacccc ctttgactaa gacgtaaaag aaaagccctg
    15721 ccatctacat gacagggacg aatgtgttta tccgcggtac cacccaattt cgggcagttg
    15781 cccgcaactc tcgtctttaa agcaaaaaga cacttttatt tcattttttc agtcattagc
    15841 aaccagtttt gacacatttg tggacttctc agcgccgcca ctttctgtaa aatgtgggat
    15901 tcaaaacacc tctaatggct ctattgtagc aagtttttct cggaaatgca aggctcaaga
    15961 aaaattactt gaacttgatg ccattttctt tcaatttctt gatggtagct gggccgattc
    16021 ctttcaaggc aaggagttct ttttcagtcc agtttttgaa atctgaagca gacttgattc
    16081 cttcatcata gaaagtcttg gcacgatcaa gtggaagtcc acccaagttt gctgcaaaat
    16141 cttctacaga attagctact ttttgagctt gctctttggt agcttctact gcttgttcaa
    16201 cttttttaga aacagcctta ccagctgcgc ttgctgactg ttctgcacgc ttcacgactt
    16261 tttttgtctc atctacaacc ttagtcacag tacttgaaaa ggcacctgcg cgacggaggc
    16321 catttcgtaa ttgtttttta cgattgagtt tctttgacat gataaaatcc tttcaataaa
    16381 aaatccctgc tctgacaagg atttaacata aacattctat caagatttta gtgaaaaatc
    16441 aagaatcttt ctcgtttaat aatttcgctc accaaacaag ccttctggca aatcatggaa
    16501 tcccttgcct gctttgaagt tttcaaactt accaagcaag aactgataag catccaagcg
    16561 actctcgtag cgaatcatgg catctttggc acgctcgtcc tggtcttctt cgtaaacttt
    16621 ttgactttcc ttgtgcgcca tgtcgttaat catgatttgg gtattgaccc aagcttcaaa
    16681 ttccttcata aattcttgtt cgtaactcat tttttctcct tattctaatc cacgaaaata
    16741 gtccgtatca atctcacggt agggctggat atcctgaaca tactccaaca ctccttggaa
    16801 ttctccgtct tcatcgtgta ctgcagcata ggtgatgtgg acaaacttac ctcgcgactc
    16861 agacttgaac cacatttcat acttgtcctt ggtcccctca cgaagcccct tcatgatagt
    16921 tttgaccttg tccaagtact taggcggatg gcagagttcg acattgcgcc cgacttgaga
    16981 cggcgtccgt ttgaaaatca tctcatcagc tggcgtattg tcattgtaat actggaaaat
    17041 atcatcttta ttgacaaagg taatctccat agggaggtga ttgaggatga gattggcctg
    17101 ctcgactgaa agatagccat taccaaaagc ctgttgacta tggcggtcca gcacagcttc
    17161 cttttcctta ggggtaaagg taatggtaaa atggccttct ggcgtatcta tgacttgttg
    17221 aacttgacct tctgccgtat ctagctgtac aggctcctct gcaatctttt cctcaataaa
    17281 gctctgtcgt tctggcaccc atttctctga cggacggatg atggcatagc cataggcatc
    17341 gctctcctcc gcaatctgaa gccagtcatc ctgagtaaaa gactcaagga gaatcatgag
    17401 gaggatggac tcttccttga aaatcatact ttcaaactct gtcgcaaaag cttcaaaagc
    17461 ttcctttaca ctgctaattg acacttctgg tagtgacttg gctatcgtta gagctgtttg
    17521 aaagagttcc ctaatctgat catccactcc ccacataact ttgggaggtg aatcgtgtcc
    17581 atagcgctcc atgataggaa agaagagttc ttccttacgt tggtaatgga tgtcaaattg
    17641 acccacaagt cccatctgac gcaccaaacc cttacgcatc tccgccagca tttcctcgtc
    17701 ttccatagac tcataggtat ctaacaatct acgaatgcga atcaaggccg cacggagagc
    17761 cagattttct tctttgaaga cacgaactgg gtgacctgga tgctcagtat ctgaaacttc
    17821 gacacctttg atagcatttt taaaaagatt ggcatggaca tcacagagtt ccataacatc
    17881 ttcaaaggtg acacctgagt ctgagttcat cagctcgtgc tccataaggg aaatctcaat
    17941 ggctgagaca cccgcaaagg tcgcatcaaa acgctcctga accgactcag gagaggcgcc
    18001 attatgcaat tctaataaaa tatcccgtag gacatgaatc cgttcatctg ccattagtct
    18061 aatccaatca cttcgtagcc attcgcttcc agtgtgcgta caatcttgtc cataggagtt
    18121 cctgctagct tagaaccttg tttaagtgat actttacgac caactgtatt gcgcatcaag
    18181 ggattggcaa ggggtttaaa acccagctcc actagaattt ccaagacttc tggatgcttg
    18241 tccaccactt ctgcaacagg aattgacaca tcgatgatat tgtccatgac gacctccatt
    18301 atatgttcta atactcaatg aaaatcaaag agcaaactag aaagctagcc gcaggttgct
    18361 caaaacagtg ttttgaggtt gtagatagaa ctgacgaagt cagctcaaaa caccgttttg
    18421 aggttgtgga tagaactgac gaagtcagct caaaacaccg ttttgaggtt gtggatagaa
    18481 ctgacgaagt cagctcaaaa caccgttttg ag
//
LOCUS       DAVKLX010000041        17980 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKLX010000041 DAVKLX010000000
VERSION     DAVKLX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.041"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..265
                     /locus_tag="VW325_001571"
     CDS             <1..265
                     /locus_tag="VW325_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734936.1"
                     /translation="VLTLIFLIKICADTTIKFLFIRLFDTSTLMKRRIFFLFALDVIV
                     YLFLGYLLVIQKAGYLFSVLLLFSNFSVPFIKEKEYELFKNSK"
     gene            1391..1894
                     /locus_tag="VW325_001572"
     CDS             1391..1894
                     /locus_tag="VW325_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734937.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDFASHTISVIQTGVFILEDFRNILLYNDVIERISLYEFIGDIFYSKLTSYC
                     IVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTN
                     VKEMKWL"
     gene            2180..4273
                     /gene="pspA"
                     /locus_tag="VW325_001573"
     CDS             2180..4273
                     /gene="pspA"
                     /locus_tag="VW325_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEU9734938.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKSEAAKKAYEEAKKKAEDAQKKYDEDQKKTEEKAENEKKAAADLNEATEVH
                     QKAYVRYFEIQRAKDSKKYKNNRDKYNKDLAEADQKIKDTKTVLDEKQSKFYAVRAVV
                     VPEAKELAVTKQKAEETKKGAEVAKEKYDKAAQEVEVAKKEVEAEEAELDKKVAELQN
                     KVADLEKEIADAEKTVADLEKEVAKLEKDVEGFKESDGEYAKFYLEAAEKDLATKKAK
                     LAEAKIKAATKKAELEPELEKAEAELENLLSTLDPEGKTQDELDKEAAEAELNKKVEA
                     LQNQVAELEEELSKLEDNLKDAETNNVEDYIKEGLEEAIATKQAELEKTQKELDAALN
                     ELGPDGDEEETPAPEAPAEQPKPEKPAEETPAPAPKPEKSADQQAEEDYARRSEEEYN
                     RLTQQQPPKAEKPAPAPAPKPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGS
                     WYYLNSNGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWLQYN
                     DSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWAK
                     VNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVN
                     TTVDGYKVNANGEWV"
     gene            4674..5795
                     /gene="mnmA"
                     /locus_tag="VW325_001574"
     CDS             4674..5795
                     /gene="mnmA"
                     /locus_tag="VW325_001574"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9734939.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            5936..6391
                     /locus_tag="VW325_001575"
     CDS             5936..6391
                     /locus_tag="VW325_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9734940.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNRKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKSQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            6401..8314
                     /gene="mnmG"
                     /locus_tag="VW325_001576"
     CDS             6401..8314
                     /gene="mnmG"
                     /locus_tag="VW325_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9734941.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMILPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQDKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            8503..8592
                     /locus_tag="VW325_001577"
                     /pseudo
     CDS             8503..8592
                     /locus_tag="VW325_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8693..10372)
                     /locus_tag="VW325_001578"
     CDS             complement(8693..10372)
                     /locus_tag="VW325_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9734942.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(10374..10607)
                     /locus_tag="VW325_001579"
     CDS             complement(10374..10607)
                     /locus_tag="VW325_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9734943.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            complement(11425..11577)
                     /gene="cibB"
                     /locus_tag="VW325_001580"
     CDS             complement(11425..11577)
                     /gene="cibB"
                     /locus_tag="VW325_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9734944.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(11579..11764)
                     /gene="cibA"
                     /locus_tag="VW325_001581"
     CDS             complement(11579..11764)
                     /gene="cibA"
                     /locus_tag="VW325_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9734945.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            11942..12625
                     /gene="tsaB"
                     /locus_tag="VW325_001582"
     CDS             11942..12625
                     /gene="tsaB"
                     /locus_tag="VW325_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9734946.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPR
                     QKEGLVVPIMDARRNNVYAGFYENAKPVLPEAHLSFAEVLEQVKDAEQVTFVGEVGAF
                     VEQIQEQLPQASYQETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            12622..13059
                     /gene="rimI"
                     /locus_tag="VW325_001583"
     CDS             12622..13059
                     /gene="rimI"
                     /locus_tag="VW325_001583"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9734947.1"
                     /translation="MIEIKRIQQQPDLAKAIYAVMIAVYPVSPWTLEQIQADLFQDQT
                     WYALAYDGAEVIGFLAVQETLFEAEVLQIAVKGAYQGKGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIADRKAYYHDPVEDAIIMKREIDEG"
     gene            13049..14059
                     /gene="tsaD"
                     /locus_tag="VW325_001584"
     CDS             13049..14059
                     /gene="tsaD"
                     /locus_tag="VW325_001584"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9734948.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(14099..15405)
                     /locus_tag="VW325_001585"
                     /pseudo
     CDS             complement(14099..15405)
                     /locus_tag="VW325_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15681..16032)
                     /locus_tag="VW325_001586"
                     /pseudo
     CDS             complement(15681..16032)
                     /locus_tag="VW325_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16139..16432)
                     /locus_tag="VW325_001587"
                     /pseudo
     CDS             complement(16139..16432)
                     /locus_tag="VW325_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            16675..17881
                     /locus_tag="VW325_001588"
                     /pseudo
     CDS             16675..17881
                     /locus_tag="VW325_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            17874..>17980
                     /locus_tag="VW325_001589"
     CDS             17874..>17980
                     /locus_tag="VW325_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9734949.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSY"
BASE COUNT         5553 a         3249 c         4025 g         5153 t
ORIGIN      
        1 tgtactgact cttatctttt tgataaaaat ttgtgcagat actacgataa aatttttatt
       61 catcagatta tttgacacaa gtactttaat gaagaggaga atattttttc ttttcgcatt
      121 ggatgttata gtttacttat ttttaggata tcttttagtg attcaaaaag caggctattt
      181 gttttcggtt ttacttcttt tttctaattt ttcagttcca tttatcaaag aaaaggaata
      241 cgaattattt aaaaatagca agtaatagaa atatggatta aatcttagaa cggcttaaaa
      301 tcagacatga taaaggaaag ctatctaggt aaccaagtac tataaacaca gtcgaggatc
      361 acttgttagg attacatttt aatcatgctt aataagaatg gagaattcct atggggattg
      421 tattactctg aagttttgaa taccttagtt ctcgtgattt tgagctattc tagctttaga
      481 atccttcaga acgtaaaatt ttaagcagtc aaagacttag aatagcccga aaacatttat
      541 tttatagcaa ttctaagatt agaattgctc atatatatgt atttgagctg ctcgatagtt
      601 aggacagctc tattctgaga aatctgacct atcaatgctt cagaatagta tttcgcaaat
      661 ggtttttatt gaagaggtga ttgttttatg atagtctaaa ataaaactga tttatggagt
      721 aaatggagaa tcgtcacatg aaacattttt ttgcaggaat tggtgagata aaacttgttg
      781 gctacttatt gtatatatgt gtaggactag cccctctttt tcatatatat gtcataggtt
      841 cggaactgag ttttgtgaaa atagtgttat ctattttggg agttatattt gtaagtatgt
      901 taactattgc tcgaatttat cggacttttc tataaagaat agtttggact tgaactgtat
      961 ctaaaaacag aggttggtcg attatcagcc tctttcttgt cgttgaaaag agtgagatat
     1021 attgactttt gaaaaaaatg tcataattga gttgggataa acctaaaagt aatatagtag
     1081 attgaatctg gaatagtccg ttatgactgt taaaatattt ctagaaatta atttgacttt
     1141 cctaatcgat ttgttcacat cttgtttcaa tctactatac tttctcgtta aaccgttcgg
     1201 caattcatgg tgacatggac acatagtagt aagcacacta tgtggcttgg cagagctaaa
     1261 aactgttccc ttgcgataag cctaataagc acaacatagg gagttagaga agccgactct
     1321 aatcaatcac tttgggcagt agtgagaact gccccgtgct ttttattttg agaaaatatg
     1381 gagtttgtcg ttgaaattac ttgattgtat tttggattat caagaaagat tcaatggaaa
     1441 aacatgtcaa gtatcaacga attataagta tttagagact ttcaaagtga atttttgctt
     1501 gactgattta catcatttat ttggcttaca caaaatcaca cgagattttg ctagtcatac
     1561 gatatctgtt attcaaactg gtgtttttat tttggaagat tttagaaata tcctattgta
     1621 caatgatgta atcgaaagga tatctttata tgagtttatc ggcgatatct tctattctaa
     1681 gctaacaagt tattgcatcg tagctaagga tttatctaaa aatactatga aattggacgt
     1741 catatttttt gaggataaaa ataaaagatc cgcagtttta ggtttacgaa gagataaaag
     1801 cggagtattt aaaccagtta ctctacattt tacaagcgct aagaaatatg ctaaagttcg
     1861 taaaacaaat gtgaaagaaa tgaaatggct ataaagattg tccgcagcct taagcttgcg
     1921 ttttttgttg tttaacgaaa ttaaaaaaca agctctctca tcggaaatgt ttttatagga
     1981 atgaaggaag atgatgctat gtttaaaaag agaatcgaaa agggcaaaag tagtgtcttt
     2041 atctttttgg agtagaagct tatgatatag aaatttgtaa taaaaatgta atataaaaca
     2101 tttgacaaat atttacgggg ggagtatact taatataagt atagtctaaa aatgactatc
     2161 agaaaagagg taaatttaga tgaataagaa aaaaatgatt ttaacaagtc tagccagcgt
     2221 cgctatctta ggggctggtt ttgttacgtc tcagcctact tttgtaagag cagaagaagc
     2281 ccccgtagct agtcagtcta aagctgagaa agactatgat gcagcagtga aaaaatctga
     2341 agctgctaag aaggcttacg aagaagctaa aaagaaagca gaagacgctc agaaaaaata
     2401 tgatgaggat cagaagaaaa ctgaagagaa agccgaaaat gaaaaaaagg cagcagcaga
     2461 cttgaacgaa gccacagaag tgcatcaaaa agcatatgta aggtattttg aaattcaacg
     2521 agcaaaagat tcaaaaaaat ataaaaataa tcgtgataag tacaataaag atctcgctga
     2581 agcagatcaa aaaataaagg atacaaaaac agtattagat gaaaagcaat ctaaatttta
     2641 tgctgtaaga gcagttgtag ttcctgaagc aaaggagtta gctgttacta aacaaaaagc
     2701 ggaagaaact aaaaaaggcg ccgaagtagc taaggagaaa tatgataagg cagctcaaga
     2761 ggtagaagta gcgaagaaag aagtagaggc agaagaagct gagttggata aaaaagttgc
     2821 tgaacttcaa aataaagttg ctgatttaga aaaagaaata gctgatgctg aaaaaacggt
     2881 agcagattta gaaaaagaag ttgctaaact tgaaaaagat gtagaaggtt tcaaagaatc
     2941 agatggtgaa tatgccaaat tctaccttga agctgctgaa aaagacctag ctactaaaaa
     3001 agctaaatta gcagaagcta aaattaaagc tgctactaaa aaagctgaat tagaacctga
     3061 attagaaaaa gcagaagctg aacttgaaaa cctattatct acactagacc ctgaaggtaa
     3121 gactcaggat gaattagata aagaagctgc tgaagctgag ttgaataaaa aagttgaagc
     3181 tcttcaaaac caagttgctg aattagaaga agaactttca aaacttgaag ataatcttaa
     3241 agatgctgaa acaaacaacg ttgaagacta cattaaagaa ggtttagaag aagctatcgc
     3301 gactaaacaa gctgagttag aaaaaactca aaaagagtta gatgcagctc ttaatgagtt
     3361 aggccctgat ggagatgaag aagaaactcc agccccagaa gcaccagctg aacaaccaaa
     3421 accagaaaaa ccagctgaag aaactccagc tcctgcacca aaaccagaga agtcagcaga
     3481 tcaacaagct gaagaagact atgctcgtag atcagaagaa gaatataacc gcttgactca
     3541 acagcaaccg ccaaaagcag aaaaaccagc tccagctcct gcaccaaaac cagagcaacc
     3601 agctcccgca ccaaaaacgg gctggaaaca agaaaacggt atgtggtact tctacaatac
     3661 tgatggttca atggctacag gttggctaca aaacaacggt tcatggtact acctcaacag
     3721 caatggtgct atggctacag gatggctcca atacaatggt tcatggtatt acctaaacgc
     3781 taacggcgct atggcaacag gttgggctaa agtcaacggt tcatggtact acctaaacgc
     3841 taatggtgct atggctacag gatggctcca atacaacgat tcatggtatt acctaaacgc
     3901 taacggcgct atggcaacag gttgggctaa agtcaatggt tcatggtact acctcaacgc
     3961 taatggtgct atggcaacag gttgggctaa agtcaacggt tcatggtact acctaaacgc
     4021 taacggtgct atggcaacag gttgggctaa agtcaacggt tcatggtact acctcaacgc
     4081 taacggttca atggcaacag gttgggtgaa agatggagat acctggtact atcttgaagc
     4141 atcaggtgct atgaaagcaa gccaatggtt caaagtatca gataaatggt actatgtcaa
     4201 tggtttaggt gcccttgcag tcaacacaac tgtagatggc tataaagtca atgccaatgg
     4261 tgaatgggtt taagccgatt aaattaaatc atgttaagaa catttgacat tttaattttg
     4321 aaacaaagat aaggttcgat tgaatagatt tatgttcgta ttctttaggt acctatctta
     4381 tgatttcagg aaatgtcatt aaaaaaacga ctccttttct ctaacttgaa aaatagatta
     4441 gagaaaatgg gttgttttat ttattatagt tatttgaatg aagataagaa gaaggtatac
     4501 tcacatcatt cacataatct gtatattgac tataagtttt aaaaaacaat ttttaagctc
     4561 ttccttgtct tctctaacca agcgtgttat aatgaatact gctcaagcga ccttcaatcg
     4621 tgaagcacac acgaccttca atcgtgaata aacgaataga tgggagactt accatgagtg
     4681 ataactctaa aacacgtgtt gtcgtgggga tgagtggtgg tgttgattcg tcggtgacgg
     4741 ctcttttgct caaggagcag ggctacgatg tgatcggtat cttcatgaag aactgggatg
     4801 acacagatga aaacggcgtc tgtacggcga ccgaagatta caaggatgtg gttgcggtgg
     4861 cagaccagat tggcattccc tactactctg tcaattttga aaaagagtac tgggaccgcg
     4921 tttttgagta tttcctagcg gaataccgtg cagggcgcac gccaaatccg gacgttatgt
     4981 gcaacaagga aatcaagttc aaggcctttt tggactatgc catgaccttg ggggcagact
     5041 atgtagcgac tgggcactat gctcgagtgg cgcgtgatga ggatggcact gttcacatgc
     5101 ttcgtggcgt ggacaatggc aaggaccaga cctatttcct cagccaactt tcgcaagaac
     5161 aactccaaaa aaccatgttc cctctgggac atttggaaaa gcctgaagta cgcagactag
     5221 cagaagaagc aggcctttcg actgctaaga agaaagactc gacagggatt tgctttatcg
     5281 gagaaaagaa ctttaaaaac tttctcagca actacctgcc agctcagcct ggtcgcatga
     5341 tgactgtgga tggtcgcgat atgggcgagc atgcaggtct tatgtactat acaatcggtc
     5401 agcgtggcgg actcggtatc ggtgggcaac acggcggtga caatgcccct tggttcgttg
     5461 tcggaaaaga tctaagcaag aatattctct atgtaggaca aggattctac catgattcgc
     5521 tcatgtcaac tagcctagaa gccagtcaag tccactttac tcgtgaaatg ccagaagagt
     5581 ttacgctaga atgtacggct aaattccgtt accgtcagcc tgactctaag gtgaccgtcc
     5641 atgtcaaagg agacaaggca gaagttatct ttgcggaacc acaacgcgcg attacaccag
     5701 gacaggcagt tgtcttttac gatggcgaag agtgtctagg tggcggtttg attgacaatg
     5761 cttaccgcga tggacaagtt tgtcagtaca tttagattga caaattttct caatttgcta
     5821 caataataac agcaatagaa atgatggtca aagctcatgg atgttgcagg cttttttgtc
     5881 ctgcacttct ttgtagtttt gactgttttt gtgtcgttta agggaaagga caagaatgac
     5941 tcaacaagac tttcggacaa aagtagacaa tacggttttt ggtgttcggg cgacagcttt
     6001 gattcttcaa aatcgcaagc ttctagtcac caaagacaag ggcaagtatt acattatcgg
     6061 cggtgcgatt caagttaacg aaaaaacgga agacgcggta gtccgtgaag tgaaggaaga
     6121 actgggtgtt aaatctcaag ctgggcagct agcttttgtg gttgaaaatc gttttgaagt
     6181 ggacggcgtt tcctatcaca atatcgagtt tcattatctg gtggatttgc ttgaagatgc
     6241 cccgttgacc atgcaggaag atgagaaaag gcagccctgt gagtggattg acttggataa
     6301 gcttgaggat atccagctag ttccagcctt tttaaaaacg gccctaccag attgggaagg
     6361 ccaactaaga cacattcatc ttgaggaata ggagagaaac atgacttatc attttactga
     6421 agaatacgat attattgtaa ttggtgcggg acacgctggg gttgaggctt ccttggccgc
     6481 tagccgtatg ggctgtaagg tcctgcttgc gaccatcaat attgaaatgc tggctttcat
     6541 gccttgtaat ccctctatcg gtggttctgc caaggggatt gtcgtgcgtg aagtcgatgc
     6601 cctcggtggc gagatggcca aaaccattga caagacttac atccagatga agatgctaaa
     6661 cacagggaag gggccagctg tccgtgccct tcgtgcgcag gctgacaagg aactttactc
     6721 taaggagatg cgcaagacgg ttgaaaacca agaaaatctg acccttcgtc aaaccatgat
     6781 tgatgagatt ttggtggaag atggcaaggt tgtcggtgtg cgtacagcca cccatcaaga
     6841 atatgctgct aaggctgtta ttgtgacgac agggactgct ctccgtgggg aaattatcat
     6901 cggagacctc aagtactcat caggtcctaa ccacagcttg gcttctatta acctagctga
     6961 caatctcaag gaactgggcc tcgaaatcgg tcgtttcaag acaggaaccc ctccacgtgt
     7021 caaggcttct tctatcaatt acgatgtgac agaaatccaa ccaggagatg aagcacctaa
     7081 tcacttctca tacacttcac gtgatgagga ttatgtcaag gaccaagtgc catgctggtt
     7141 gacctacacc aatggtacca gtcatgagat tatccaaaac aacctccacc gtgcgcctat
     7201 gtttacaggt gtggtcaagg gagtgggacc tcgttactgt ccatcgattg aggacaagat
     7261 tgtgcgcttt gcggacaagg aacgtcacca actcttcctt gagccagaag gacgcaatac
     7321 agaggaggtc tatgtccaag gactttcaac cagtctgcct gaggatgtcc aacgtgactt
     7381 ggttcattcc atcaaaggtc tggaaaatgc agagatgatg cgaacaggtt atgctattga
     7441 gtatgacatg atcttgcctc accagttgcg tgcgactttg gaaaccaaga aaatctcagg
     7501 acttttcact gctggtcaga caaatggaac atcaggttac gaagaggcag caggccaagg
     7561 gattatcgca gggatcaatg cggctctgaa aatccaagat aagcctgagt tgattttgaa
     7621 gcgtagtgac ggttacattg gggtcatgat tgatgatttg gtgaccaagg gaaccatcga
     7681 accctaccgt ctcttgacca gtcgtgctga ataccgtctc attctccgtc atgacaatgc
     7741 tgatatgcgc ttgactgaga tgggacgcga gattggcctt gtggatgatg aacgctgggc
     7801 tcgttttgaa atcaagaaaa atcaatttga taatgagatg aaacgcctag acagtatcaa
     7861 actcaagcca gtcaaggaaa ccaatgctaa ggttgaggag atgggcttca aacccttgac
     7921 agatgcagtg acagccaagg aattccttcg ccgtccagaa gtttcttacc aagatgtggt
     7981 agccttcatc ggaccagctg cagaggattt ggatgataag attatcgaat tgattgaaac
     8041 agaaatcaag tacgaaggct atatttcaaa agccatggat caggttgcca agatgaaacg
     8101 tatggaagaa aaacgcattc cagccaatat tgactgggat gacatcgatt ctattgcgac
     8161 ggaagctcgt cagaagttca aactcatcaa tccagaaacc atcggccaag ccagccgtat
     8221 ttcgggggta aacccagcag atatttctat tttgatggtg tatctggaag gtaaaaatcg
     8281 tagtatttct aaaactcttc aaaaatcaaa atgatacgtc gtcggcttct tacgaatgag
     8341 ttcaaagctt ggctttgatt catctccagc ctcccatagt ttcccgaact atgggagcta
     8401 actcaagtta tgcattttcc tagccttctg gtctgatttt cattgagttt taatctcaaa
     8461 agaaagcata gagaaaaaca gctccgaaga cggggctgtt ttgttgactt gtactcaatg
     8521 aaaatcaaag agcaaactag gaagctagcc gtaggttgct caaagcactg ctttgaggtt
     8581 gtagatagaa ctgacgaagt cagctcaaaa caccgttttg aggttgtgga tagaactgac
     8641 gaagtcagta acatatatac ggcaaggcga agctgacgtg gtttgaagag tattattctt
     8701 catctggtgt gaggatcatc gggatgatga tcggttcacg ttcggtattt tcatagagga
     8761 aggggcgaat agcgttgaca atggcaccat tgacagattg cacgctagca tccttatttt
     8821 tcagtgcgat acgaatggca ttgaagagga tacgctggct ttggcgaatc aagtcgccag
     8881 actctctcat gtagacaaag cctcggctga ggatgtctgg accagataga atcatctgcg
     8941 atttgaagtc aacagttgcg actgccagaa cgacaccgtc ttcagataga tcgcgacgat
     9001 ctttgaggac agctgcgcca atttcaccga tacgatttcc atcgacatag atatcttggg
     9061 cgttgaaatg acctgcgata cgagctgagt cagcagtaag ggcaagcaca tcgccattgc
     9121 tcatgataaa gatattgtcc ttctcaacac cagtatccac tgctagtcca gcgtggactt
     9181 tttgcatgcg gtattcaccg tggacaggca tgaagtattt tggcttaatc aagcagagca
     9241 tgagtttttg ctcttgctga ccaccgtgtc cagatgtatg gatattgttc actttaccgt
     9301 ggataacttc gacaccagct tcagaaatga tgttaatcag cttgttgacg ctagtagtgt
     9361 ttccagggat gggactagaa gagaagataa cggtatcacc tggttgtaat tgtacttgac
     9421 ggtgggttcc gttggcgata cgagagaggg ctgccatagg ctcaccctga ctacctgtac
     9481 agaggataag aacttctcct gcaggataat ctttgatttc atttggctcg ataaaggttc
     9541 ccttaggagc tttgatgtag ccaagatcga ttccgttgac aatggccttt tccatagaac
     9601 gaccaaagac cgcaatcttg cgtccagtct taacagcagc ttctgttgcc tgctggagac
     9661 ggaagatatt tgaggcaaag gatgcaaaga tgatacgtcc ttcaatacct tggataatct
     9721 tcataatgga ctgaccaacg actttttcag agttggtaaa ggttggtact tccgcatttg
     9781 tcgagtcaga caggagacag agcacacctt cttcaccaag cgcagccata cgatgcaagt
     9841 ccgcaggttc tccaactgga gtaaagtcga acttaaagtc ccccgtacag acgattttcc
     9901 cttgaggagt atgaatgaca atccccaaag gctctggaat agagtgagtc gttctaaaga
     9961 aagttgcctt gagattttta aaggtcaact cggtgttgtg gttgatttcg taaagtttgg
    10021 cgttgcgcaa gaggccgtgt tcttcgagtt tcccacggat caaagccaag gcaagcggtc
    10081 cagcgtagat agggacattt gcttgcttga gtaggaacgg aatcccacca atgtggtcct
    10141 cgtgtccgtg tgtgattaaa acagccttga cgcggtcgat attgtccacg atgtaagagt
    10201 agtcaggaat gacatagtcg ataccaagca agtcatcttc tgggaattta atcccagcat
    10261 cgacgatgat aatctcgtct tggtattcaa ttccgtaagt gtttttcccg atttctccta
    10321 gaccaccgat ggcaaaaacg ccgacttctt caggtttaag agtgtaggcc atattagaac
    10381 tccgtaattt cgaaggcgcc agtttctttt tcgtaatcga gcaatttgtc agacaagagt
    10441 tcgatatatt cgatattgta ctctgggcga ttttcttcga caagttggcg agcagtgata
    10501 cggccctcaa gttctgagct ggcatcgatg tctaggtaaa gcgcgcgtgt tgtttcacgg
    10561 cgtgggctac gttcttttgt ttcttgataa aaaactttgt aaatcatata gttcctttct
    10621 atttacaggt cagcttattc caaactttta gcagatattt gcttgatttc attccatttt
    10681 gtgtctagat tgaccttgat tcgttacaat tatttcaatt ataccacaaa atgaaaaatt
    10741 agtaaaagac ggaaacgaga aagttagggg gcaatttctt tggtaagcgt ttacaaaaat
    10801 aaagaaaatg tattatgttg ataacggaag tgaaatttat tgcttgtcat aattccatat
    10861 agattttaag tcaaggaggt tgccctatgt ataacttatt tgcttttgct atcggagttc
    10921 gtttgttagc ttttatcgtt tttgttgctt gcgtttatgg aggaaatctt ccatttgcca
    10981 aactggaacg atgacaaacc aagttcttgt ggaaaattac ctttgtgact ctctactcgc
    11041 tttttctcct cctcgtgccc tatgtcgttt ggtttgtatt ttactttgga ttaaatgctt
    11101 agatgccctg cttatgctgg ggttttttgt ttggggatgt agagatagta gatttacgac
    11161 acgaaaattc caccccatct atcaagatga ggtggtcttt gcatttttct aaaatgacat
    11221 gacaatggaa ttttcaaagc ttattatttt tatgggagtc attgttctgc tcaaaaatag
    11281 agttgaagca taaaaataaa acaatacaaa cagataaatc gaatggtaaa gggatagtag
    11341 taatgttcaa atcattgaga atgctgagca aactgtaaga gatgaagaca ataaaattta
    11401 tccttctaaa aatgtgtttc ttatctagca tgacttacca aactttttac gaaggtcttt
    11461 tccaatggta taacctgcca taaataaagc gataccaaca cctacaccaa agccaccctt
    11521 gatttcttgc aattccttat tagaaatttc acgatagttg ttcaaatttt tcatcatatt
    11581 aatctcctcc taaaaaataa ccagctgcca aaccagaacc tataagtgct gttccacctg
    11641 caatagcctt ccaagatact gctactccaa agataaccaa gggagcgaga ccgccatcaa
    11701 tatctgataa ttcattttca gataaggata aaaattgatt gtcaagaatg tcaaaatttg
    11761 tcattgaact ttcctccttt tttcttgttc acttttatat tcggaaaaga aaaggaaaag
    11821 tgagcatttt tttaaattat ttttagttaa gtgagggcac tcttgtctgg gggcaaagaa
    11881 aattcccatg tgccagcttt tgtagtataa tagtaagcag acaaaaagat aggaatgtgt
    11941 tatgaaagta ttagcttttg atacgtccag caaggctctt tctctcgcta ttttagagga
    12001 caagcaggtt cttgccgaga cgacgattaa tattaagaaa aatcacagta ttactcttat
    12061 gcctgccata gattttttga tggcaagttt ggattggaca cccaaggatt tggaccgaat
    12121 cgtggtagct gaagggccgg gtagctatac aggcttgcga attgcggtag caactgccaa
    12181 gaccttggct cataccctga acattgagtt agttgggatg tctagtctat tggctctggt
    12241 gccacgccaa aaagaaggct tggtggttcc tatcatggat gcgcgtcgca acaatgtcta
    12301 tgcaggcttt tatgaaaatg ccaagccagt cttgccagaa gcgcatctgt cttttgcgga
    12361 agtgctagag caggtcaagg atgctgagca ggtgaccttt gtcggagaag ttggagcctt
    12421 tgtggaacag atccaagaac aactaccaca agccagctac caagaaacct tgccaaatgc
    12481 agctaatcta gctctttggg cttgggacaa agaagcagac tccttgcacg actttgtgcc
    12541 aaattacctc aagcgtgtcg aggctgagga aaactggctc aagaaccata ccgagtctgg
    12601 cgagtcttac attaaacgcc tatgatagaa atcaaacgaa tccaacagca gcctgatcta
    12661 gctaaagcca tctacgctgt tatgatagct gtttacccag tcagtccttg gacgctggaa
    12721 caaatccaag ctgacctgtt ccaagaccag acttggtatg ctctggctta tgatggggca
    12781 gaagtgattg gctttctagc tgttcaggag actctctttg aagcagaagt cctgcaaatc
    12841 gctgtcaaag gagcctatca gggtaagggc attgcgtcag ccttgtttgc tcaattgccg
    12901 acagataagg agattttcct cgaagtcaga aagtcaaatc aacgagcgca agcattttac
    12961 aagaaagaaa aaatggcggt catcgctgat cgaaaggcct actaccatga cccagtcgag
    13021 gacgccatta tcatgaagag agaaatagat gaaggataga tatattttag catttgagac
    13081 atcctgtgat gagaccagtg tcgccgtctt gaaaaacgac gatgagctct tgtccaatgt
    13141 cattgctagt caaattgaga gtcacaaacg ttttggtggc gtagtgcccg aagtagccag
    13201 tcgtcaccat gtcgaggtca ttacagcctg tatcgaggag gcattggcag aagcagggat
    13261 taccgaagag gatgtgacag ctgttgcggt tacctacgga ccaggcttgg tcggagcctt
    13321 gctagttggt ttgtcagctg ccaaggcctt tgcttgggct cacggacttc cactgattcc
    13381 tgttaatcac atggctgggc acctcatggc agctcagagt gtggagcctt tggagtttcc
    13441 cttgctagcc ctcttggtta gcggtgggca cacagagttg gtctatgttt cggaggcagg
    13501 agattacaag attgttgggg agacacgaga cgatgcagtt ggggaggctt atgacaaggt
    13561 cggtcgtgtc atgggcttga cctatcctgc aggtcgtgag attgacgagc tggctcatca
    13621 ggggcaggat atttatgatt tcccccgtgc catgattaag gaagataatc tggagttctc
    13681 cttctcaggt ttgaaatctg cctttatcaa tcttcatcac aatgccgagc aaaagggaga
    13741 aagcctgtct acagaagatt tgtgtgcttc cttccaagca gcagttatgg acattctcat
    13801 ggcaaaaacc aagaaggctt tggagaaata tcctgttaaa accctagttg tggcaggtgg
    13861 tgtggcagcc aataaaggtc tcagagaacg cctagcagcc gaaatcacag atgtcaaggt
    13921 tatcatcccc cctctgcgac tctgcggaga caatgcaggt atgattgcct atgccagcgt
    13981 cagcgagtgg aacaaagaaa acttcgcagg ctgggacctc aatgccaaac caagtcttgc
    14041 ctttgatacc atggaataaa gagtggctct ttgtcaagtg tagtgggtag gcgaaaagct
    14101 acaatctgga gagtacgaaa ttcgtactct cttttttgat gttcaaagtc atcaaaatcc
    14161 gttttttaaa attgttaaag ttccggaaac caaaggcttt gcgcttgata accttaatca
    14221 aattattggt cgcttcaagt ttagcgttgg aataaggcag cttaagtgcg ttgataatct
    14281 tatctctatc ccttaaaaaa gtccaaaaga ccgtttgaaa aagcggatga accgttggca
    14341 gttcttgctc aatcaatcca aagaaatggt cggcattctt ctcttgaaaa tgaaagagca
    14401 ggagttgata gagttcgtag tgaacctgta atccctcaga gtaagaaagc agcttatctc
    14461 gtacttcccg atgggttaag tgcattcgaa acgtgggctg atagaatcgt ttctgacaga
    14521 gcttgaagct atcctggtgt agtaaatcag tccagtggac tgatttagcc cgagcctaga
    14581 aacgaaaaag cgagggttcc aaaagcgctt gagcgcccga tactccaagg attttcggtc
    14641 aaattggttc atgatttgga ttcgtactcg gttcatagct cggctcagat gttggacaat
    14701 gtggaagcgg tcaagaacaa tcttcgcctt tggaaagagt tgcttagcga gccgataata
    14761 agggctgtac atgtccatgg tgatgacctc gaccagctcc cgaacctctc tctgatagcg
    14821 ttggaaatgg tttcgaatca ccgcatgagt tcgcccgtct aaaattgcga ggatggattt
    14881 ggactcaaaa tcttgggcaa tgaagctcat tttgctcttt ttgaagctat actcatccca
    14941 actcataact tttggcaact tggtccaatc ggtttcaaac ttaaattccc tcagttttcg
    15001 gatgacggtg gaagttgaga ccgccaatct gtgggcaatc tcagtcatag actgcttttc
    15061 aaggaggagc tgagcgattt tttgtctcac catgttggaa atctggcaat ttttcttgac
    15121 aatggatgtt tgagaaacga ccaccttaag gcaattcttg cactgaaagc ggcgcttttt
    15181 gagatgtagt accgtgggta aaccctgaca gtcgagaagc ggaattttag aggctttctg
    15241 gaagtcgtat ttgatcatct tcccttgaca atgaggacaa ggaggagcgg gggaatccaa
    15301 ctttgcctga acgactagat gggtttggta tttcagaaca gacaagattt tgatattttt
    15361 gtctttcaat ccgagcaaat ctgtggtatt cttaagttgt tccatatgaa ccctttctaa
    15421 tgagttggtt agacgctttt cattataggt catatggggc tttttttcta caagaaacga
    15481 ccctataatt cctggggtgg gattacccac tacagaaatt atagagccaa aacattccaa
    15541 agtcttgtct gactctggaa tgttttctgt tcacagttgt tttgtaactg cctaataagt
    15601 aaccgcgcta taacgaggta tctaccgaga ttgtcaattc tccagtatac tgttataaaa
    15661 aacaaaactg gaggattttt ttatgtcccc ccatcgtccc atcgactgtt ccgtagctgc
    15721 ccaataacgg agtatagtcg gctgaaacaa gaataggaca aaagtgcctc aagaaaagca
    15781 tcgtaatttg acaatattat tctgagatgt ttttgatgta agcccatatt ttctcaatgg
    15841 gattatactc gggtgaatag gtaggaagtg gtaacagtct atgccctgct ccttacacat
    15901 gttcattctg tgaaaccttg cattgtccat aatgataagg gatggtgtat ctaaagtagg
    15961 tagtaagaat ttttggaagc aagcttcgaa aaagtcactc gtcatcgtat ctttataggt
    16021 catcggagcc atagtatatt gaaacaaaaa agggcattag tacttctata acagtatcgc
    16081 aatctctggt gtgcgttgtc tcgatcttaa ttcaaataat tatatcttct tccagagaga
    16141 ctaactgcct gctcaaggct cgatcatatt cgagataaaa ataggtctca acccctgtct
    16201 cataaatata aataggagtc aggtagctta agtgattcaa ttctttaagg aaccgattta
    16261 ctttttctgg gtcttgttcg cagtaggtac agctcttttt tagactatat cccatagcct
    16321 ttagagcgta atgaatagtt gttggaggac agttgaattc agcagctatt tcagtcaaat
    16381 aagcatcggg atgagtttca atatagatct ttaatttatc tctatcaact ttttcaatta
    16441 atagtggaag ttttcttaaa gttagtgtgt tgaaacgttt ttatacagtg ctaaaatttg
    16501 aagtgtagtg cagatgtatt tgtaatagta ttatagcaat tctttggaga aggtcgagac
    16561 aagagaaaat aggtatcttt gagatataag catgatttac tataaaatta ttgagttatt
    16621 cataaaaatt tctctatcaa atatcataat gcgtcgttta aggagatggt tacaatgcaa
    16681 tatagttgtg gaaaaataaa tattaatatc cctgatggat atggagacat taaggatata
    16741 gtgttttcgg ctcatattat agttaggtat aataatggac attgtggagg tatagatccg
    16801 catataattg gactttgtaa aaagcagatc agaaggatgt ctctatatcc tattttgata
    16861 atcgtatcta gagattcaaa ggttattgat gattataaaa atttagatat tgcctatgtt
    16921 gattgtactc aatgttcaaa taattttgaa actgccttac acgttaaaaa tattttaaaa
    16981 ttattaaaaa tccaacttat acactgtcat ggctactcaa ctaattattt tttatatatg
    17041 ttaaaaaaac ttgacaagaa tggatttgga aaggttaaga cagtgataac atgccatgga
    17101 tgggttgaat ataacttaaa aaagaaattc ttgacttatt ttgatttttg gacatattct
    17161 atgggagatg cttttatttg tgtatctgaa actatgaaaa aaagattgga gagtataata
    17221 aaaaaataag aagatagttg caataaataa cggaattaat gtatcaaata gcgatttaga
    17281 tgtagtaggt gttcaagact ttaaaaaaga attttgtatt ccaaataaca aaaaaatcat
    17341 ttgttatgtt ggaaggttgg atccagaaaa aaggccggat agattccttg aatttgcaga
    17401 aaaattattt ttagtcagag aagatgtgat ttttattatg gctggaaatg gtagtatgtg
    17461 ggctgctttg aaagaaaaaa tttgccattt gaaatgtaga gataatttca ggttacttgg
    17521 tgaaatctat ccagctacta tagtttacca aatatcggat ttattgtata ttccttctga
    17581 tacagaagga atacctatgt gtgttttaga gtcaatgtca caaggaactc cggttttagc
    17641 tagtaatgtt ggcgggttaa gtgaaattat tgaacatagg gttgatggat ttttatttga
    17701 gaaggaagat gttgagggag tgtgtgcttg tgctaatttt ttactcaatg attctgagta
    17761 tttgaaatat ataggtgaga atagtaaatc aaaaataaga aaacattttt ctgtgcaaaa
    17821 aatgtttgta gaaaccatga gagtatatga tgaattatta gagaagagta gtcatggata
    17881 ggaatataga tcaagaatta gtaagtatta taataccaac acataatagg tatgaatctc
    17941 tgattagagc tgtgaaaagt tgtttacatc aaagttataa
//
LOCUS       DAVKLX010000042        17470 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKLX010000042 DAVKLX010000000
VERSION     DAVKLX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17470
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.042"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            334..1380
                     /gene="pheS"
                     /locus_tag="VW325_001590"
     CDS             334..1380
                     /gene="pheS"
                     /locus_tag="VW325_001590"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9734950.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1380..1889
                     /locus_tag="VW325_001591"
     CDS             1380..1889
                     /locus_tag="VW325_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9734951.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1966..4368
                     /locus_tag="VW325_001592"
     CDS             1966..4368
                     /locus_tag="VW325_001592"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9734952.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(4436..5437)
                     /locus_tag="VW325_001593"
     CDS             complement(4436..5437)
                     /locus_tag="VW325_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9734953.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5593..6039
                     /locus_tag="VW325_001594"
                     /pseudo
     CDS             5593..6039
                     /locus_tag="VW325_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6165..6431
                     /locus_tag="VW325_001595"
     CDS             6165..6431
                     /locus_tag="VW325_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9734954.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            6692..8941
                     /gene="metE"
                     /locus_tag="VW325_001596"
     CDS             6692..8941
                     /gene="metE"
                     /locus_tag="VW325_001596"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9734955.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNSITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9005..9871
                     /gene="metF"
                     /locus_tag="VW325_001597"
     CDS             9005..9871
                     /gene="metF"
                     /locus_tag="VW325_001597"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9734956.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10556..12769
                     /gene="pnp"
                     /locus_tag="VW325_001598"
     CDS             10556..12769
                     /gene="pnp"
                     /locus_tag="VW325_001598"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9734957.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12785..13402
                     /gene="cysE"
                     /locus_tag="VW325_001599"
     CDS             12785..13402
                     /gene="cysE"
                     /locus_tag="VW325_001599"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9734958.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13414..14298
                     /locus_tag="VW325_001600"
     CDS             13414..14298
                     /locus_tag="VW325_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9734959.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14385..15728
                     /locus_tag="VW325_001601"
     CDS             14385..15728
                     /locus_tag="VW325_001601"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9734960.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15721..16107
                     /locus_tag="VW325_001602"
     CDS             15721..16107
                     /locus_tag="VW325_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9734961.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16111..16995
                     /locus_tag="VW325_001603"
     CDS             16111..16995
                     /locus_tag="VW325_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9734962.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(17042..>17470)
                     /locus_tag="VW325_001604"
     CDS             complement(17042..>17470)
                     /locus_tag="VW325_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9734963.1"
                     /translation="RKLSDKHFYRPTFRMHLTNKEILDKLLSYSQDLKHHYQLYQLLL
                     FHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKKKIVNALQLPYSNAKLEATNN
                     LIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         5193 a         3386 c         3989 g         4902 t
ORIGIN      
        1 ttttcatctt atttcaatcc gctataaagt tagataagaa taagctaaca agcagaagta
       61 agataggtct tgcaagaatg gtaaaaatct gataaactaa gtggtaattg aatagaaatc
      121 tgaaagacta gtagcccaag ggaagtatat cagggaggag agccgtgact gcaagctctc
      181 tatatgaaag ctgggtgaat tcacttgcgc atggatttag aaatgaaggt ctgacttgtc
      241 agatgaaaac ggatggtacc gcgtgtcaac gctccgagtg gagtttttgg catgtggttt
      301 tctttttatc tacgagagac tgatggagga aatatgtcaa ctattgaaga acaattaaaa
      361 gcgcttcgcg aagaaacgct gactagcttg aagcagatta ctgctggaaa tgaaaaagag
      421 atgcaagatt tgcgtgtctc tgtccttggt aaaaagggtt cgctcactga aatcctcaaa
      481 gggatgaaag atgtttctgc tgagatgcgt ccaatcatcg ggaaacacgt caatgaagct
      541 cgtgatgtct tgacagctgc ttttgaagaa acagctaagc tcttggaaga aaagaaagtc
      601 gcggctcaac tggctagcga gagtatcgat gtgacgcttc caggtcgtcc agttgcgact
      661 ggtcaccgtc acgttttgac acaaaccagt gaagaaatcg aagatatctt catcggtatg
      721 ggttatcaag tcgtggatgg ttttgaagtg gagcaagact actataactt tgaacgtatg
      781 aaccttccaa aagaccaccc agctcgtgat atgcaggata ctttctatat cactgaagaa
      841 atcttgctcc gtacccacac gtctccagtt caggcgcgtg ctatggatgc ccatgatttt
      901 tctaaaggtc ctttgaagat gatctcgcca gggcgtgtct tccgtcgcga tacggacgat
      961 gcgacccaca gtcaccaatt ccaccaaatc gaaggcttgg tagttgggaa aaatatctct
     1021 atggctgatc ttcaaggaac gcttcagttg attgtccaaa aaatgtttgg tgaagagcgt
     1081 caaattcgtt tgcgtccatc ttacttccca ttcacagagc catctgttga ggtggatgtt
     1141 tcttgcttca agtgtggtgg agaaggctgt aacgtatgta agaaaacagg ttggatcgaa
     1201 attatgggag ccggtatggt tcacccacgt gtccttgaaa tgagtggtat cgatgcgact
     1261 gtatactctg gttttgcctt tggtcttgga caagagcgtg tagctatgct ccgttatgga
     1321 atcaacgata tccgtggatt ctaccaagga gatgtccgct tctcagaaca gtttaaataa
     1381 tgattagaaa agtagaaatg gcagatgttg aggtgttggc taaaattgcc aaacaagcct
     1441 ttcgtgaaac ctttgcgtat gataatacgg aagagcagtt acaggaatac tttgaagagg
     1501 cttatagtct gaaaactttg tcaactgagt tgggaaatcc tgactctgaa acctatttca
     1561 ttatgcatga ggaggagata gctggttttc tcaaagtcaa ctggggaagt gctcaaactg
     1621 agagagaatt agaggacgct tttgaaattc aacgcctcta tgtgctacaa aaattccaag
     1681 gatttggact aggtaagcaa ctgtttgaat tcgcacttga acttgctaca aaaaatagtt
     1741 tttcttgggc ttggctaggt gtttgggagc ataatacaaa agctcaagcg ttttataatc
     1801 gatatggttt tgaaaaattt agccaacatc attttatggt tggtcaaaaa gtagatacgg
     1861 attggttact gagaaagaaa ttaaggtaag aagaggattc ttctattgaa atgataggaa
     1921 aggaaaagaa ccagtgtggc aatggtcatt ctggagaact aaattatgct tatatcttat
     1981 aaatggttaa aagaattggt ggacattgat gtgccatcac aagagttggc tgaaaaaatg
     2041 tcaactacag gaatcgaggt agagggtgtc gaatcaccag ctgctggtct ctcaaaaatt
     2101 gtcgtcggtg aggtcttgtc ttgcgaagat gtgccagaga ctcacctcca tgtttgtcag
     2161 gttaacgttg gcgaagaaga gcgtcagatc gtttgtggtg ccccaaatgt gcgtgctggg
     2221 atcaaggtca tggtggctct tccaggagct cgtatcgctg ataactacaa aatcaaaaaa
     2281 ggaaaaatcc gtggtttgga gtcacttgga atgatctgtt cacttggtga attgggaatt
     2341 tctgactcag ttgtgcctaa ggaattcgca gatggcatcc aaatcttgcc tgaagatgcc
     2401 gtgccaggtg aggaagtctt ttcttaccta gacttggatg atgaaatcat cgaactttcc
     2461 atcacaccaa accgtgcaga tgccctttct atgtgtggag tggctcacga agtggcagcc
     2521 atctatgaca aggcagtcaa ctttaaagaa tttactctaa cagaaactaa tgaagctgcg
     2581 gcagatgccc tttctgtcag cattgagaca gacaaggcgc cttactatgc agctcgtatc
     2641 ttggacaatg tgaccatcgc accaagtcca caatggttgc aaaatcttct catgaacgaa
     2701 ggaatccgtc ccatcaataa cgtagtggac gtgaccaact acatcctgct ctattttggt
     2761 caaccaatgc atgcctttga cttggataac tttgaaggga ctgacatccg tgtgcgtgaa
     2821 gcgcgtgctg gtgaaaaatt ggtgaccttg gacggtgaag aacgtgactt ggacgtgaat
     2881 gacctagtca tcactgtcgc agacaagcca gtagcccttg caggtgtcat gggtggtcaa
     2941 gcaacagaaa tctctgaaaa atctagtcgt gttgtccttg aagctgctgt tttcaatggc
     3001 aaatctatcc gtaagacaag tggtcgcctg aaccttcgtt ctgagtcatc ttctcgcttt
     3061 gaaaaaggaa ttaatgtggc aacagttaat gaagcccttg atgcggcagc tagcctgatt
     3121 gcggaacttg caggtgcgac ggtgcgtaag ggcatcgttt cagcgggtga gcttgatact
     3181 tcagatgtag aagtttcttc aacccttgct gatgttaatc gtgtcctcgg aactgagctg
     3241 tcttatgctg atgtagaaga cgtcttccgt cgtcttggct ttggtctttc tggaaatgca
     3301 gacagcttta cagtcagagt cccacgtcgt cgttgggata tcacaatcga agctgacctc
     3361 tttgaagaaa ttgctcgtat ctatggttat gaccgcttgc caactagtct accaaaagac
     3421 gatggtacag caggtgaatt gacagccaca caaaaactcc gccgtcaagt tcgtactatt
     3481 gctgaaggag caggtttgac agaaatcatc acctatactc taacaactcc tgaaaaagca
     3541 gttgagttta cggctcaacc aagtaacctt acggaactca tgtggccaat gactgtggat
     3601 cgttcagtcc tccgtcaaaa tatgatttca ggtatccttg ataccgttgc ctacaacgtg
     3661 gctcgtaaga ataaaaactt ggccctttac gagattggaa aagtctttga acaaacaggt
     3721 aatccaaaag aagaacttcc aaatgaaatc aacagttttg cctttgcctt gacaggcttg
     3781 gttgctgaaa aagatttcca aacagcagca gttccagttg atttcttcta tgctaaggga
     3841 atccttgaag ccctatttac tcgtttggga ctccaagtaa cctatacagc aacatctgaa
     3901 atcgctagcc ttcatccagg tcgtacagcc gtgatttcat tcggtgacca agttcttggt
     3961 ttccttggcc aagtgcatcc agtcactgcc aaggcttacg atattccaga aacgtatgtg
     4021 gctgagctta acctttcagc tatcgaagct gcgcttcagc cagcgactcc atttgtagaa
     4081 atcaccaaat tcccggcagt cagccgtgac gttgcccttc tcctcaaggc agaagtgact
     4141 catcaagaag ttgtagatgc tatccaagct gccggcgtga aacgtttgac agatatcaaa
     4201 ctctttgacg tcttctcagg tgagaaattg ggacttggta tgaagtcaat ggcttatagc
     4261 ttgaccttcc aaaatccaga agatagctta acggacgaag aagtcgcacg ctatatggaa
     4321 aaaatccaag catcgctcga agaaaaagtc aatgcagaag tgcgttaaac gatagtatga
     4381 tacaaattcc ccctctagtt tatgaacact agaggggtga ttttatataa aataattaaa
     4441 atgtgatttc tgtaaaaata catctatgat cagagttttg aaaacttaca atgtctaaat
     4501 ctttaacata gtagtggttt ttaggcaata aaatatgatc tattgttgca ttaaaaagtt
     4561 ttggactttg gctattccaa gttcctcttt caaaaggtgg cagtacattt aatgcgtccc
     4621 tatgagagct tatttttgca agtgctccat gacgcatagt tgcattaaaa tcacctgcaa
     4681 taatagcctt tggatatttt gaagccaatt gattatgaat gatgtttaag tcttgcttcc
     4741 agatttccat taaacctggc agaggaggag cagtatgcaa ggcaatgata tctggtatat
     4801 tttgttttct cgaatgtaat acaattgtcc cgaaccgtgt tgtatgaaaa gttttagctt
     4861 ctgtatagaa accataactt ttcttgacaa tcacagtcac aggagctatt ccactattgg
     4921 taggtggaga agtgaaaata tcatagttgg ccatagaaag tccaacttga tgaaacaata
     4981 gtttgattct ctggttttct tgctcacctc tgatattggt agctagttca gggaatatag
     5041 ccatatcggc gtcaaaatgg ctaaaaattc gctcaatatg ttgtgcttct atttgattag
     5101 cgacgttcca agttactaat tttaaggttt tagtcttatt tgatgcagaa gaaagggttt
     5161 tatctgttcc gaataatagt aatatgagcg atagtaagag caaaatgcca gagtaaaacc
     5221 gtatttgttt atttttcttg taaaaagata gggaaaaaaa tacgaaactt aaaatagcag
     5281 ccatagttgc agtagctaag gggaaggcat aaatctgtgc caagataggt tgggtagata
     5341 gccaaatgtt aagattagaa gatgggaaaa aagaataagt tataataggg atagaaaaga
     5401 gaatgaccga aagggcagag agaaatgttt taatcattgt gtgagtacct caatttgcaa
     5461 tggttgctcg gagctaatgt gactttgtga ctttacttga ttgatattct ttttcttgtc
     5521 ttcctcatag attaagtaca acccaagtaa aaaaagtcca atttaagatt gaaatgatta
     5581 aaaggcacag aaatgcaaga caattatact acaaaaggca aacatttgac aatcgatagc
     5641 cgtcgcttaa tcgaacgatg gaaaaaagaa ggaaaatcaa atagagaaat tgcctctcta
     5701 cttggaaaag ctcctcaaac tatccatact gaaatcaagt atgggacagt ccgacaatgt
     5761 cttggaaaag ggcgcttcaa agagatttat tctgccgact acgctcaaca gtcttatgaa
     5821 aataatcgca agcactcggt caagaaatca agcttgacta agaaactaaa ggaaaagatt
     5881 ctccactatc ataaccaaaa attttcgcct gaaatgatgg tgatggctaa aggggttaac
     5941 gtgggaattt caaccattta ctattggatt catcgtggaa aattggggtt aagcaagcag
     6001 gatctgcttt atcctagaaa aggaaaagcg cttaagaaat aggctagcac caactttaaa
     6061 cccgctggtc aatctatcga atcataattc gctcattttt ttaaaaatac atagacgtaa
     6121 aaaaaagagg gttaagtgat gaagaatagt gctattggga gtaattggaa ggatgtccat
     6181 ctgagctctt tagcaaggag gaaatccttg aaagtgatat gcgagtggct atcatgagcg
     6241 agttgattga ggctaggtat gaacaaggaa tcagtcagaa aaagctggaa gaagtcagtg
     6301 gaataagcca gcctgtcata gctaggatgg agacaggaga gagcagtctt cagttggata
     6361 cggtcttaaa agttctaacc agtctaggaa agacactagc agtcgttcca cttgaacagg
     6421 ggaaaagttg actagaatca gttatagtct tttctaataa caggtcatag tcacttataa
     6481 gaattactaa taacatgttt tagtattcta attggaatat agctgtaaag taagaattac
     6541 aaaggatttg agacgtttgc ttcaagtctt tttcttttgt ccgaaacaga tatatagttt
     6601 caaactatat caaaccctaa ttttttacaa ttatacagac gctttctttt ctgttaagat
     6661 agtttcaaca acaaattttg gaggacacat catgtcaact acaatcatcg gtttccctcg
     6721 tttgggcgaa ttccgtgaat taaaatttac aactgaaaaa tactttagaa aagaaatctc
     6781 agaagaagaa cttttagcag cagcaaaaga cttgcgtgct aaacactgga acattgtcaa
     6841 agaaaaaggc atcactgaaa ttccatcaaa tgacttttct cactatgata acttcctaga
     6901 tgcggctttc cttttcaacg tggtgcctgc atccgttcaa aacttggact tgtctgacct
     6961 tgagcgctac ttcgctttgg gacgtggtta ccagggagaa aaaggggacg ttcgcgccct
     7021 tccaatgaag aaatggttca acactaacta ccactacatc gttcctaaat ttgaaaaaga
     7081 cactcaagta aaattggctg gtcacaagat tttcgatgag ttccaagaag caaaagaact
     7141 tggattgaac actcgtcctg ttcttgtagg tccattcact ttccttcaat tgtcagactt
     7201 tgaagaaggc gtgaaagcag acgacttcgt agatagctta gtggttgctt accaagaagt
     7261 ttttgctaaa ttggctgaac ttggtgcgac tcgcatccaa ttggatgaag cggctcttgt
     7321 aaaagacttg acagctgaag aaaaagctct cttcttgaac ctttacaaca aactcttggc
     7381 tgacaaaaaa ggtcttgaag tcttgcttca aacttatttc ggtgatgttc gtgacgtata
     7441 cgctgacctt gtaaacttgc cagtagatgc tatcggtctt gactttgttg aaggtaagaa
     7501 aactcttgaa ctcgttaaag gtggcttccc agctgacaag actctctatg taggtattgt
     7561 caatggtaaa aacatctggc gcaacaacta cgaaaagagc ttggctgttc ttgagcaaat
     7621 cccagctgaa aacatcgttt tgacaagctc ttgctcactt cttcatgtgc catttacaac
     7681 tgctaatgaa gaatttgaac cagcactctt gaaccacttt gcctttgcag ttgaaaaatt
     7741 ggatgaaatc cgtgacttgg atgctatccg caatggtcaa ggttcagaag ctcttgcagc
     7801 caacaaagaa ctctttgcga ctgagcgtgt tggtgaaaat gctgaacttc gtgcgcgtat
     7861 cgctggcttg acagacgcag actacactcg tttgccagcc tttgcagaac gtgaagctat
     7921 ccaagaagaa gctttcaaac ttccagctct tccaacaaca acgattggtt cattccctca
     7981 aacaaaagaa gttcgtgcca aacgtttggc ttaccgtaaa ggtgaattgt ctcaaaaaga
     8041 gtacgatgct ttccttgctg aaacgattga cgaatggatc aagtggcaag aagatattga
     8101 ctttgatgtc cttgttcacg gtgaatttga gcgtaatgac atggttgagt acttcggtca
     8161 aaacttgtca ggatacctct tctctaaaaa tggatgggta caatcatacg gtatgcgtgg
     8221 ggtaaaacca ccaatcatct ggggtgatgt cactcgtctt aactctatca ctgttaaatg
     8281 gtctagctat gcacaaagtc gtacaaacaa acctgttaaa ggtatgttga ctggacctgt
     8341 taccatcctt aactggtcat tcccacgtga agacatctct atcaaggatt caactcttca
     8401 aatcgccctt gctatcaagg atgaagtact tgaccttgaa gctgctggtg tgaaaatcat
     8461 ccaaatcgac gaggctgctc ttcgtgaaaa attgccactc cgccgtagcg actggtacga
     8521 agactacctt gactgggcta ttcctgcctt ccgcttggta cactcaacag tagcgccaga
     8581 cacacaaatc cacactcaca tgtgttactc agaatttaca gatatcatcc cagctatcga
     8641 taacatggat gcagacgtta tttcctttga agctagccgt tcaaaccttg aaatcttgga
     8701 cgaactcaaa gcgaaaaact tccaaacaga agtgggacct ggggtttacg atatccactc
     8761 acctcgtgta ccaaatgaag gcgaaatcga caacacaatc gaagccatcc ttgctaaagt
     8821 gccaagcaag aaagtttgga tcaaccctga ctgtggtttg aaaacacgtg gtatcccaga
     8881 aacaaaagaa agcttgatcc gccttgttga agcagctaaa gctgcgcgtg agaaattgta
     8941 agattggatt tctctccata cagtggttgt tcaaaaagaa atcaactaga aaaggttatt
     9001 acatatgtca cgccaaacac cgtcactctc atttgaagtg ttccctccaa acccagccgt
     9061 gggtaatggt aacattattt cagccttgca ggatatgcag gagttggctc cccattttat
     9121 cagtgtaact gccagcaata ataaatttaa tatcaaggaa acgacggttc gtttggctga
     9181 ctttatccaa aatgacttgg cgattccgac cattgcccac ttgccagcta tttatctaac
     9241 taaggacaag gttgctgaaa ctattgctga cttggacaaa gttggggtgc agaaaatctt
     9301 ggctcttcgt ggggatatta ttccagacgt agaaccacaa aaggatttcc gctatgcaac
     9361 cgacttgatt gagtttatca aggaacaagc ccctcacttt gatattattg gagcttgcta
     9421 tccagaaggg catccagatt cgccaaatca gatttcagat attcaaaatc ttaagaagaa
     9481 agtggatgca ggctgttcga gcctcgtaac tcagctcttc tttgataatg agcgcttcta
     9541 tgattttcag gacaagtgta ttttggctgg gattgatgtt cccattcatg caggaattat
     9601 gccaattctg aatcgaaatc aggctctccg actcttgaag acttgtgaga atatccatct
     9661 tccacgcaaa tttaaagcca tcttagacaa gtatgagcat gaccctgagt cgctcagagc
     9721 agcaggactt gcctatgcag tggaccaaat cgtggacttg gtaactcagg atgttgccgg
     9781 tgtgcatctc tatacgatga ataatgctga tacagcaaaa tacatccatc aagcaaccca
     9841 tgccttgttt aatcaccagt ctctaggata ataaaagcaa accattcttc tcaggtgagg
     9901 ggaatggttc ctttttaatg gtaaagacct cactttttaa gaaaaatatg ataaaataaa
     9961 ttctgtacgt acttgataca aagatgaagg tattaaataa tacaatgcat ttaaactgta
    10021 agatttaacg acacgagtga gtcaaatcga tgttatttga cgagagagtt agtaaagcat
    10081 ttagctaatc gcccgatagc gattagcgtg aaagctttag atactttagt attaaacctt
    10141 tctaatgatg tatcttgaaa actgaacttg cctacaactc tgtggaaaaa gataaatctc
    10201 cctaggagca gtcgctccgt cgttcgattt cctattttcc tttgagttgc ttgacggctt
    10261 ggtatcttga tgacttgaat tttggtctca tttcttagga aaaaagataa gcttcctagc
    10321 actcgtcgag tgcgacgtca ccttcctatt tttctacaga aatgttcggc aagccgaacc
    10381 gtccaaaata tcttgtgtaa ttgaacacgg ccgaaaagct gtgtaaaaaa gataaactgt
    10441 cttgtcttca tcgaagactt cgtcagtttc ctatttttac tttgcttttg acgtccttgg
    10501 tatcttgatc tttgtaggca aggcgtataa tttcatcaat ccaaagggga ttaaaatggc
    10561 aaaacaagtg tttcaaacga cttttgcggg tcgtgagtta attgtagaaa ctggtcaggt
    10621 tgctaagcaa gcaaatggct ctgttgtcgt acgttacggt gagtcaactg tcttgactgc
    10681 tgccgttatg tctaagaaaa tggcaactgg ggatttcttc ccactccaag tcaactacga
    10741 agaaaaaatg tatgcggctg ggaagtttcc tggtggcttt atgaaacgtg aaggacgtcc
    10801 ttcaacagat gcgaccttga cagcgcgttt gattgaccgt ccgattcgtc ctatgtttgc
    10861 ggaaggtttc cgtaatgaag tccaagtcat caatacagtg ctttcttatg atgaaaatgc
    10921 atctgcacca atggctgcta tgtttggttc atctttggca ctgtctattt cagatattcc
    10981 atttgacgga ccaattgctg gggtacaagt gggatatgta gatggccaaa tcatcatcaa
    11041 cccaagtcaa gaacaagcag agcaatctct tcttgaattg acagtagctg gaaccaagca
    11101 cgctatcaac atggtagagt ctggtgccaa agaattgtca gaagaaatca tgttggaagc
    11161 gcttcttaaa gggcacgaag ctgtcaaaga attgattgcc ttccaagaag aaatcgttgc
    11221 tgctgtcggt aaagaaaaag cagaagtgga attgcttcac gtggatgctg aattgcaagc
    11281 tgaaatcatt gcagcctaca acagtgacct ccaaaaggca gttcaagtag aagagaaatt
    11341 ggcccgtgaa gctgcaactc aagcagtgaa agaccaagtg actgccgttt acgaagaaaa
    11401 atatgcgaac cacgaagaat ttgaccgtat tatgcgtgat gtggctgaaa tcttggaaca
    11461 aatggaacac gcagaagtgc gacgtttaat tacagaagac aaggtgcgtc ctgatggtcg
    11521 taaggtcgat gaaatccgtc ctttggatgc ggttgttgac ttccttcctc gtgtacatgg
    11581 ttcaggtctc tttactcgtg ggcaaactca agctctttca gtcttgacct tggctccgat
    11641 gggagaaact caaatcattg atggtttgga tccagagtac aagaaacgct ttatgcacca
    11701 ctataacttc cctcaatatt ctgtagggga aacaggtcgt tacggtgcgc caggtcgtcg
    11761 tgaaatcggt cacggtgccc ttggtgagcg tgctcttgct caagtcttgc caagcttgga
    11821 agaattccca tacgctatcc gtctagtagc agaagttttg gaatcaaacg gttcttcatc
    11881 tcaagcttct atctgtgcgg gaactcttgc ccttatgact ggtggtgtgc caatcaaggc
    11941 gccagtagct ggtattgcta tgggacttat ctcagatgga aataactaca cagtattgac
    12001 agatatccaa ggtttggaag atcactttgg agatatggac ttcaaggttg caggtactcg
    12061 tgatgggatt acagcccttc aaatggatat caagattcaa gggattactg cagaaatctt
    12121 gacggaggct cttgctcaag ccaagaaagc gcgttttgaa atccttgatg tcattgaagc
    12181 aaccattcca gaagttcgtc cagaattggc tccaactgct ccgaaaattg atacgatcaa
    12241 gattgatgtg gacaagatta agattgtcat cggtaagggt ggagaaacca tcgacaagat
    12301 tatcgctgaa acaggtgtta agattgatat agacgaagaa ggaaatgtgt ctatctactc
    12361 tagtgaccaa gatgctatta accgtgccaa agaaattatt gctggtttgg ttcgtgaagc
    12421 caaagtggat gaagtttacc gtgctaaagt cgttcgtatc gagaaatttg gtgcctttgt
    12481 taacctcttt gataagacag atgcccttgt tcatatctct gagatggctt ggactcgtac
    12541 caatcgtgta gaggatttgg tagaaatcgg ggatgaagtt gatgttaagg ttatcaagat
    12601 tgatgaaaaa ggccgtatcg atgcctctat gaaggctctt ctacctcgtc cgccaaaacc
    12661 tgagcatgat gaaaaaggtg aaaagtctga gcgccctcac cgcccacgtc atcacaagga
    12721 tcacaaacct aagaaagaat ttacagaaac accaaaagat tcagagtaag aaaaggagaa
    12781 atgtatgggg tggtggcacg aaaccattga tatcgtaaaa gaaaatgatc cagcggcccg
    12841 caccactttg gaggttttgc tgacttatcc aggtgtcaag gccttggcgg cccaccgtct
    12901 ctcgcatttt ctctggaagt acgacttcaa actcttagct cgtatgcaca gtcagttttg
    12961 gcgcttttgg actcagattg agattcatcc aggagcccag attgattcag gtgtctttat
    13021 tgaccatggt tctggtctgg tgattggaga gacagcgatt gttgaaaaag gcgttcttct
    13081 ctatcacgga gtgactctcg ggggaacagg gaaagactgt ggcaaacgcc atccgactgt
    13141 acgaaaggga gccctcatat cagcccatgc ccaagttatc gggcctgtgg aaattggtga
    13201 aaatgccaag gtcggtgctg cagcagttgt cgtagcagat gtacctagtg atgtgacggt
    13261 tgtcggtatt ccggccaaga ttgtccgtct tcatggtaag aaagatgagc ctgttattca
    13321 cgaagtcgaa gaaaaacggg agtattatgt caataaactc gagcaggcta aagatgccag
    13381 tcacagatcg tctggtttgt agaggatacc tatatgattc aagcaagaaa caagttaagc
    13441 caagaggagc tatctgaggc gaaaaaagta attaactgtt gccaaaacta tgacggtacc
    13501 tatcgcgatc cctatctctc taacatgctt aattttgacc caaacatgcc cgcctttttc
    13561 ctttattatg aaaaaggcaa acttgttggt ttattaactg tctatgcaga tgaccaagat
    13621 gtagaagtga cgatattggt tcatccaggt catcgccgtc aggggattgc gcgtgcattg
    13681 tttactagtt ttgagagaga aacagcttct ttccccattc ggtcagtcac ttttcagaca
    13741 gaacgtattt ttttagagaa tcatcctgac tttgccagta actggggact aatcgaggat
    13801 gaagagacag aaacttggtt aggtaaggat agaagacctt atccgttagc aaatgtttcc
    13861 catcttgaag ttttgttagc agatagttcg tatcaggatc aaattagtca attaaaattt
    13921 caggcatttt cagaggaaca tgaatcgaga gaagttgtgg atagatatgt cgctgaagct
    13981 ctgaaggatc cagaaagtcg cttatatatt ttgttaaaag acggtcaggt tattgggact
    14041 tgcacggtag atttatcgac taatacgaat tacttctacg gtttagcaat atcggaacct
    14101 gaacgtggga aaggctatgg aagctactta gcaaaatccc tcgtcaacca actaattgag
    14161 caaaatgaca aggaatttca gattgcagtg gaagatagca atgtaggtgc caaacgtttg
    14221 tatgaaaaaa ttggctttgt caaacagaca caggtggttt atctgaatga gaaaggagca
    14281 agggattccg aagtgtagag acattcggac tgaaatttac ttgagctctt agtttagtga
    14341 tgaaactaac agttcttgga ttttagcttt cctgattatg atttatgatt aaaatctatg
    14401 acaccatgtc tcgtgatttg cgagaatttg tcccgattga ggacggcaag atcaagatgt
    14461 atgtttgtgg gccaaccgtg tacaactata ttcacgtggg aaacgcccgt tcgacggtag
    14521 cttttgatac gattcgtcgc tattttgagt accgtgggta taaggttgcc tatatttcca
    14581 attttacaga tgtggatgat aagattatca accgtgccag ggaagaaggc atcacacctc
    14641 aggaggttgc ggataagtac atcgctgcct ttcgtgagga tgtgacggcc ttaggcgtga
    14701 aacctgcgac tcgccatccg cgtgtagtgg agtttatggc agacatcatc cgttttgtgg
    14761 aagacttgat cgagaaaggc tttgcctatg agagtcaagg ggatgtctat ttccgtgtag
    14821 aaaaatccca caactatgct aaattggcta ataaaacctt ggaagatttg gagctaggtg
    14881 cttcaggtcg taccgatgaa gaaacggctc gtaaggaaaa tcctgtagac tttgctctat
    14941 ggaaatcttc caaaccaggt gagatttctt gggatagtcc ttggggacct ggacgtccgg
    15001 gttggcatat tgagtgttca gtcatgtcga cagagatttt aggtgatacc attgatatcc
    15061 acggtggtgg agctgaccta gagtttccac accacaccaa tgaaattgcc caatctgaag
    15121 ctaaaacagg caaggctttt gctaactact ggatgcacaa tggctttgtc aatatcgaca
    15181 atgtcaaaat gtctaagtcc ttgggtaact tcattacggt acacgatgcc cttaaaactc
    15241 ttgatgggca agtacttcgt ttcttctttg cgacccagca ctaccgtaag cctatcaact
    15301 tcacggaaaa ggcagttcgc gatgccgaga ccaatctcaa gtatctgaag aatacttacg
    15361 aacaaccatt tacaggaaac gtggatgccc aagagttaca aaactttaaa gataagtttg
    15421 tagcagctat ggatgaagat tttaatgctg ccaacggtat cacagttgtc tttgaaatgg
    15481 ccaaatggat caactcaggg aactatgatg caagtgtcaa gcaagctctt gcagatatgt
    15541 tagaaatttt tggaattgtc tttgttgagg aagttttgga tgcagagatt gaagacttga
    15601 ttcaaaaacg ccaagaggcg cgtgccaatc gtgactttga gacagcagac caaatccgtg
    15661 accaattggt tactcaagga attaagctcc ttgataccaa ggatggagtg aggtggacac
    15721 gtgattgatg tcaatctcat taacgggatt gcgctagcct ttgagggaga tgcggtgtat
    15781 tctatgtata ttcgccgcca cctcatcctc aaaggtatga ccaagcccaa taaactccac
    15841 caagaggcga ccaagtacgt gtcagccaag gctcaggctc gtctgattgc tctcatgttg
    15901 gaggagcaga tcctaacgga aaaagaagaa gaaatctaca aacgtggccg caataccaat
    15961 agtcacacaa aggctaaaaa tgcagatgtc gtgacttatc gtatgtccac gggatttgaa
    16021 gcagttatgg gctatctcca tatgactgag aatctggaac gtcttgagag tttggtttca
    16081 tggtgcatcc aaaaagtgga gggctagaac atgagggcaa aagaactaca agactggttt
    16141 cctgaggctc ggatttcaga ccaaccagta gagaaagagg gctatctcac gcttccttta
    16201 gcttctcagc agtggatttt gctggaggaa gctgggctca gcgagcgtga aaagcagttg
    16261 gttgcccttt tgacccagca ggagcaggct cgttcgctaa acccttggta ttcctatctg
    16321 gttgagggca agggacaggc accgcaagtt tttaaaaaga ttcagttggt ttattgccat
    16381 ctttcttatt ttcagcagga aaatctgact tcttggctag atatgatgcg gactcttttt
    16441 ccgaattgtc agacagtgct acaggtcgga gctcaggatt atgttttcgt gcttcaacaa
    16501 gacaaataca cttctgtaag agatattttg agtgatacga ttgaagcggt tgagtatgac
    16561 tttggacttc gtctttctat catgttgggt caggtttggt ctcagacagg acatcaagcc
    16621 ctatcagact taatcaaagc tgagcgggat ttgttcaaga catggtggcg tcagggtcac
    16681 caaggtgttc atactttttc tcagctctat ctttggagta tgggagaaag actcgtggac
    16741 ttgaagccaa ttaaagaatg tctacaccag atgattttgg atcaagatca gattcaggaa
    16801 atcattctct ctctttggga aaatagtgct gttctcacta aaacagccca gcaactctat
    16861 ctgcaccgca attctctcca atacaagatt gataaatggg aagagttgac agggcttcag
    16921 ttgaaagagt tgaccgacct gaccttgtgt tatcaattga ttttaggttc tttgtcaact
    16981 atagttggtt tgtaaagaag ttaatatggc tctttgtcaa ctgtagtggg ttgaaaaaaa
    17041 gctaagctcg agaaaggaca aattttgtcc tttctttttt gatattcaga gcgataaaaa
    17101 tccgtttttt gaagttttca aagtttcgaa aaccaaaggc attgcgtttg ataagtttga
    17161 tgagattatt ggtcgcttcc aatttggcgt ttgaataggg tagttgaagg gcgttgacga
    17221 ttttcttttt gtcctttaga aaggttttaa agacagtctg aaaaagagga tgaacctgct
    17281 taagattgtc ctcaataagt ccgaaaaatt tctctggttc cttattctga aagtgaaaca
    17341 gcaagagttg atagagctga tagtgatgtt tcaagtcttg tgaatagctc aaaagcttgt
    17401 ctaaaatctc tttattggtt aaatgcatac gaaaagtagg gcgataaaaa tgtttatcgc
    17461 tgagtttacg
//
LOCUS       DAVKLX010000043        16974 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKLX010000043 DAVKLX010000000
VERSION     DAVKLX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16974
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.043"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            177..359
                     /locus_tag="VW325_001605"
     CDS             177..359
                     /locus_tag="VW325_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9734964.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            304..1152
                     /locus_tag="VW325_001606"
                     /pseudo
     CDS             304..1152
                     /locus_tag="VW325_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(1333..1584)
                     /locus_tag="VW325_001607"
     CDS             complement(1333..1584)
                     /locus_tag="VW325_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734965.1"
                     /translation="MPKTKTLAELADVILWSFDFVNAHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFLFDFGDEWRFEC"
     gene            complement(1962..3842)
                     /locus_tag="VW325_001608"
     CDS             complement(1962..3842)
                     /locus_tag="VW325_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9734966.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAGTIADIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3836..4705)
                     /locus_tag="VW325_001609"
     CDS             complement(3836..4705)
                     /locus_tag="VW325_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9734967.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHCFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAGKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNCNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4789..7434)
                     /locus_tag="VW325_001610"
     CDS             complement(4789..7434)
                     /locus_tag="VW325_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9734968.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQAECAKWGKSTQIGYFPDTFGNMGQAPQILQQSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGADGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVCTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRILVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7525..8805)
                     /locus_tag="VW325_001611"
     CDS             complement(7525..8805)
                     /locus_tag="VW325_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9734969.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEKNWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFVELDLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPLALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8976..11060
                     /locus_tag="VW325_001612"
     CDS             8976..11060
                     /locus_tag="VW325_001612"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9734970.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKSSAQVDWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSTCKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLNKTLVSTIQHQDLLKAE
                     QLIFTLSWLPNH"
     gene            complement(11100..12779)
                     /locus_tag="VW325_001613"
     CDS             complement(11100..12779)
                     /locus_tag="VW325_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9734971.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGE
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPAYG
                     NGGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELDYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGVEVS
                     GSALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQAGETEKSLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            13534..14763
                     /gene="arcA"
                     /locus_tag="VW325_001614"
     CDS             13534..14763
                     /gene="arcA"
                     /locus_tag="VW325_001614"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9734972.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            14824..15837
                     /gene="argF"
                     /locus_tag="VW325_001615"
     CDS             14824..15837
                     /gene="argF"
                     /locus_tag="VW325_001615"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9734973.1"
                     /translation="MNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHYL
                     AGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITEDA
                     DEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPAF
                     HDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLYI
                     PKV"
     gene            16002..16949
                     /gene="arcC"
                     /locus_tag="VW325_001616"
     CDS             16002..16949
                     /gene="arcC"
                     /locus_tag="VW325_001616"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9734974.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
BASE COUNT         4886 a         3863 c         3447 g         4778 t
ORIGIN      
        1 ttaagtcaag aaaaattaac ccactttaat ttctttttta acccgtatta aaaaagaaat
       61 taaagtgggt taaaaaagaa attaactgag taaaaactct cgaaattcaa ttcgctatat
      121 tatcactcaa cttgaacaaa taccagaacc acaaaaaaca gatgtaaagg aaaaatatgg
      181 acttaaaatt tgaaggggtt gatttagaat acaaaaaagc taaaaacaat cttccagagt
      241 ctttctggga aacctactca gcatttgcaa atacaaatgg tgggaaaata attctcggga
      301 tagatgaaaa aaacattgac acctaccaaa gagtcaatag actccctgcg aaattatgaa
      361 gcaagtaaac aattaactga tacacgactt aagcgtcttg ttggtgttca gcgtaccact
      421 tttgaagaga tgttagctgt attaaaaaca gcttatcaac ttaaacacgc aaaaggtgga
      481 cgaaaaccta aattaagcct agaagacctt cttatgccca ctcttcaata gtgcgagaat
      541 atcgaactta tgaacaaatt gcggctgatt ttggtattca cgaaagcaac tttatccgtc
      601 ggagccaatg ggttgaagta actcttgttc aaagtggttt tacggtttca agaactcctc
      661 tcagttctga ggacacggta atgattgatg cgacggaagt aaaaatcaat cgccctaaaa
      721 aaacaattag cgaatgattc tggtaaaaag aaatttcacg ctatgaaggc tcaagcgatt
      781 gtcacaagtc aagggagaat tgtttctttg gatatcgctg tgaactattg tcatgatatg
      841 aagttgttca aaatgagtcg tagaaatatc ggacaagctg gtaaaatctt ggctgacagt
      901 ggttattaag gactcatgaa gatatatcct caagtacaaa ctccacgtaa atccagcaaa
      961 ctcaagccgc taacagctga agataaagcc tgtaaccatg cgctatccaa ggagagaagc
     1021 aaggttgaga acatctttgc caaagtaaaa acgtttaaaa tgttttcaac aatctatcga
     1081 aatcatcgta aacgcttcgg attacgaatg aatttgattg ctggtattat caatcatgaa
     1141 ctaggattct agttttgcag gaagtctatt gaaatactaa gcctacacag actgattcca
     1201 attgtttttt agatttcaat tttaccattc ttcatagtca aaaccatcat aatctggata
     1261 ttgttctggc gacgttccaa ccgaacgtac gagataagct tcttcgtcct ctgtctcgat
     1321 ttctctcagc acttagcatt caaaacgcca ttcatcaccg aagtcgaaaa gaaacttaaa
     1381 tttttgtttg acacttaggc tatccagata gacattttct gtgtaacgtt cttcaacatc
     1441 gtcactaaca aagctaagaa agtaagaatc tgcatgactc cactcaacat tatccatgaa
     1501 aaatgcgtga gcatgagcat ttacaaaatc gaaactccat aaaatcacat ctgctagttc
     1561 cgctaacgtt tttgtttttg gaatctatat atgtcggtag caaccttttt taaatttgac
     1621 ggaaatgacc caggcttcta cagatgctgg cttttcagct tctatttctt gttttactaa
     1681 ttttaagtgt agcataatac ctccaattgc tcatcgatag aattgctctt aggaaacatt
     1741 cactttctgc tattttatca taagtttgtc taactgtagc atttgaaacc agataattct
     1801 tctgtttcac gcgaaacacc ctgcgccaag caaggtgttc ttgtttttat actcaatgaa
     1861 aatcaaagag caaactagga agctagctgc aggctgctca aaacactgtt ttgaggttgt
     1921 agatggaagc tgacgtggtt tgaagagatt ttcgaaaagt attaagtcgt ataaatcgtc
     1981 gacgctgtcg caatggtatg ccactggtta gctgtagttg ctgcgaaatc cttgtctgcg
     2041 tagaagtcag taaatggtag gatttcaact tccagctcgt cgatgcggtc aagctggcca
     2101 gccagataaa cctcgatacg gctttctgct cgtttgatgc gttgcaagag tccgcccata
     2161 cggatgtcaa ctgtatccaa accaaagacc ttgttttctt tcaaccattg gtgactaaag
     2221 agggcatgga agtcttcaat ttggcttcta agttctggta attcttgtct ggcgatttgt
     2281 tgtaaacttt ctttatcatc cgcttggtag gcctgacgaa tgcgtcgtcc cacatctact
     2341 ttgctactta aaatagcatt caactgggcc tgagtttcaa agagataagc atagtttcca
     2401 gctttttctt taatgtcagc aatagttccc gccgcctgag cgaagtgtgg cttgtcctgc
     2461 tcaggtgtca tgtgtcggtc aagtatcgga cagagaacat cctgataaaa gacatagcgg
     2521 ttgggattga tgccactgag attacctggt aggtctggta agaggttggc aaggtcaatc
     2581 tgcataaaat cctcaaccgt tagaccagta ttggtcttga agtgcgcaga caaactatct
     2641 aggtcattgc gatagctgag ttctgcccag atttgcaagc ttggtaggat agagaactgg
     2701 gcagtttcac caccattgtc tccccaaccc gttacgatga cttctttaat ctgattggca
     2761 cggcaggctt tattagcctc gatagccact agacggctaa aatggttgtg aggtgtaaag
     2821 ccaatccact tccaagctcc ccctgcaaat gcaaggtcat ggctaatctt gtgatgattg
     2881 cggaaattac ggttgtattt ttcctcgcta tcctgataat aatcccagta aaccagagtc
     2941 acacggtctt tgagacggtc taggtagaca cgagtttcct ctggaatttc cacatcacgg
     3001 tcgtactggc catccgctga catgagtttg aagaacatat cactccacat ctggcagtgg
     3061 aaaccatatt tgtcagcaat atccagcacg cgctccaagt gttggcacat gaggagacta
     3121 cgatccacaa caccgttcaa aatcaggtag cgtcccaaac caaccaagtg ggcttcgtcc
     3181 atcccgatat tgaccttgcg agtcttcagt ttagacaacg tggcaaacat gccatcaatc
     3241 aggtcataaa ccttttcttc gccaatgaga agaatgtcct ctacatcacg gagctcctgc
     3301 acttccttga caccccattt gacaaaggcc gacaagtggg ccaaggtctg gatgcatggt
     3361 acaaaggtca tgtcaaactg ttgagcatag gcttcgattt cctgcaactc ctctgctgaa
     3421 taagctccac ggaaatagcc aaagtaaggc tgtccttcaa tctggtaagt gtcttccatg
     3481 taaagctcaa aggttgagta gcccatgaga gccaagacct caatcatctg cttggcagaa
     3541 gccacattca gcaccgcatt tcgagaacag tcaaccatgt aagccaaatc ttcgtaagct
     3601 gcttgttcct caatctctac tttatcagct tctgctagaa ctgttaccaa caaggacaag
     3661 gcacgataaa gttggtgagg tttgcggtag gtcaattgat agtgaccttc ctcaccctgg
     3721 atagagatag aggcttggtc agactgagtg acagccactt ccacatctgg tagagaaatg
     3781 tgacctttta aaacctcaat agcttgcgtt tgtttgggac taagtcctgt aaatcttacc
     3841 attgcttttc ctcctgtagc cagttgacaa gggcaccgta gagatttgca tctgcgtgat
     3901 aggtgcaggc ctggataact ggtgcgaccg tgtattcttc gtaggcatct acaaagtctt
     3961 caacagcctt cttaacacct tggataaaat ctggattttg actgatagag ccacccagac
     4021 tgatgacatc tggatcgatc agatactgga tattgagcaa gccttgcgcc agattgcagt
     4081 tcatgcgctc aatggcttct tgacaaagag cattaccagc tgcggcctct tggtaaatct
     4141 tgcgaccgtc ccaatcagta tgaccagatt tttcaattac gtatcgtacc atattcccag
     4201 ttgatgctag ttgcgaccag ttattaagtt ttccagcagg ggcaagggtt gtcatgtaac
     4261 caaattctcc acccagaccg tggcgacctc gatgaagtct accattgata atcatggctc
     4321 cgccaatccc tgtcccaatc acgacacagg ctgcattttc aagctctgga tgagctagta
     4381 gttcactgag tccaacgcag ttggcatcat tttctaaatg gacaggtagc tgataagagc
     4441 taagcgcctc ataccaagaa aagcaatgga tgtagggcac cgcactgaag ccatcaatca
     4501 cacctgtctc ttgattgacc gcacctggaa cgctcatagc aatcccactg taatcctgtt
     4561 ctgacaagcg ttgatctagc cacgctagta aatcctccaa gttttcaggc gttgaaatac
     4621 ttgtcttatc cagtattttc ccatcaggag tcagactggc aaacttaatc ccagtccctc
     4681 cgatatcaat cgttgcaatg gtcattgttt ttaccataaa aaggagcgaa tcagcagtta
     4741 gttcttacct tttcgcccct cctttctttt gatgtttact ttttgaaatt aaatttcttc
     4801 ttttttgatg aattctgtac gaatctcttg tggagccaat agtcctgaat gaactggata
     4861 tggtcgctca agtaagtcaa gaatggtttg ttgatgttct gatatgcgca cattttcttg
     4921 actcatatta tagtaacgga ggacatagcc ttcttcattt tccgccactt taaaggctgt
     4981 tggacaaact tgcggtatgc tgagaacaga atggctcaag aggctaccag tcgcaaccac
     5041 gcttccttcc tgtctagcaa gctgaaggct ggtaaacggt gtctgcaagg ctttggcacg
     5101 acgataggct gagaagcgtt cttgggcttg gtggcattca agtgcaaact cgacttcaaa
     5161 ctcacgcaag cattgtgctt ctggcgttgg gaagtagccc cagtcaccta gctcacctga
     5221 tgcacgcaaa atggtcacgg caatggtgtt atccccaagg atttcgtatt cattcaatcc
     5281 cttattggat acagtcaccc ctttttcatc gtcatacaga ctgacaaaag cttgttggtg
     5341 ttgaggattt tcagggtttt cccatgaagc agctggtttg tttggtcgtg tcaccacctc
     5401 atagatactt tcagaatcat tgcttggacg cgtgttatga gtcttgacca agatacggat
     5461 acggtgatcc ttggcagtgt tagtaaagcg agtcttgaag cggatttgtg gattgtcaac
     5521 gaagacagtc aactcagttt ccagaggaat gtttgtcaat tcttctgacc gtccagcctc
     5581 acgcttcata aactcgatga taccttgttg ctcttcttct agcttttcat ccgcactgac
     5641 aggcacggtc aattcatgtt tgagcaagat tttagcatag caagctgtgt tttccaagac
     5701 ctcgtggccc ttaagctctg caaagattgg ctctgttcct tttggttgga aatagatata
     5761 ctcgtttcca atgtccccac ggtcttcaaa gcggataaag tcttcatagg cttcgtgagt
     5821 tgtcttgtca tagactgtga tgttgtcatc cacactcacc gttacgaatg gtgtatcaat
     5881 cactccgttt tggtaaatac cctcacggtg ttcttgttct ccttccagca attggaaggt
     5941 tgtccaagaa agcggtgcta ggtgaactgg aatggtcacg cgcacttgtc gagcaatacg
     6001 agcttggcgg aacttatctt ttggtaaatc gtattcaaaa ttagctccga ggtcttcgat
     6061 tgtagcctct acaggacgac catccaagtc ctccacacgg taacttggca agataagagc
     6121 agccatcttt ttgtagcctt ctgttgggtg caattccttg aaatcacaag tcgccacatc
     6181 aatcactgtg cagacagtat cgaccttatc atgcaagcct gtgttaatga cagtaaagag
     6241 atagtcactt tgagccttat ccgtagcaat tttacccttc cactcgttga gcaaattact
     6301 tttaacaaag tttcctactt ggttgacctt ggcaaaacgc gtttccatct cgcggtgaac
     6361 ttcgtccacg ctacagccac agatactatc atgtggcgca ttctgcaaaa gtgttttcca
     6421 agcataggtc aactggtcct tgtggttgtg tccaccagtg ataatagtca agggttctac
     6481 cacttgctct aggaggttgc tattttcttg gaaggcttgt tttaggtaaa tgcgggatga
     6541 agaagtgttg gcaagtgtgt accagccatc tgtttcctga ctggtcaact cgcctgtaac
     6601 agttgataag tgttcaggaa gcgcaccttc tacagcttga acatattcat caaaagaact
     6661 atgaacaaag attacatccg ggaagagttc atttgccaca cgaatggctt cgctcagatt
     6721 tttctgtaca ggctggtggt cacagccgtt catcatcaac cattggttgg tcgaagcgta
     6781 ggcacgcaca tctgacaatt tttgtttcca gaaggtcaag gcctcatctt tgtcaactgg
     6841 gatttcattc ccattactgt accagttggc aaagagaata cctaaaacac gactaccatc
     6901 cgcaccctgc cagtacattt ctgaaaactg ggaagtaaac tgctcatctt cgaggacttg
     6961 gttgtcaaat ccaatcggct tcacaccacg accaaaggct gccacgtgaa tgcctgattg
     7021 ttgaagaatt tgaggcgctt gtcccatatt tccaaaggta tctgggaaat aaccaatctg
     7081 agtagatttg ccccattttg cacattctgc ttgaccaatc aaggtatttc ggacattggc
     7141 ttcactggag atcaagtagt catcctgcaa gatgtaaaag ggaccaattt taagtttgcc
     7201 ctcgtcaatg tagcgttgga ccttgtcacg attttcaggg cgaatttgta agtagtcgtc
     7261 aaggacaata gtttgtccat ccaagtggaa actcttgaac tcagggtcat tttcaaagag
     7321 atcaaagaga ttgtcaaaca attccaccaa ctgcatacga tggctttcaa aaggcaagta
     7381 ccactcacgg tcccagtgac tatgtgagat aatatgtaca acaacatttt ccatgaagta
     7441 aaacctcatt ctaaatttaa attttaacgt tttaaatgta aacttgtgat tctgataaga
     7501 atcggttgag ctaaagcgag ccccttagcg aatatccaag taatccaaga ccaactcaca
     7561 gaacatcatg ttagcccagg agaaccattc acgagagtag agggtcggat catctacgtg
     7621 gaagctttcg tgcatgacac ctgtaccacc atcgcaggca accagctgat ccagcaagaa
     7681 ttttttctct gccttatctc ttgttgtcaa tccttggata gaaagggcga gtggccagat
     7741 atagcgatag aaggtatgag aacttccgag accgctagcg tattctcctt ggtagaagta
     7801 tggattttca gagctcagaa tggtacggcg agtggcttga tacacttcat catcgatcga
     7861 acagtagccc agatagggcg cagctagtag acttggtaca tttggatcat ccatgatgct
     7921 ggcatttcct aggccatcta cttcaaaagc gtaaatcttt tcgcccttgc tgttggtggt
     7981 gtaagcgtag ttttcgattc cttcttggat ttcatcctga agacgcttgg catcagcaat
     8041 aacactctgg ctatcagcta ggtctaattc gacaaagatt tcttgcacat aacccaagac
     8101 tactacggca aacatattag acggaatcaa gtaactatac tgacagcaat cgtcactcgg
     8161 acgaaaagct gaccaagtca tacctgtcac tgcaaagtca ggtccaaagc catcatttac
     8221 caaggtgtct tccttacggt ccgtatctcg gacaaaacga taaggagagt tcttgtggtc
     8281 ttgttcaact gtccatagat ggagaatttc cttagtcgct gcgacaaaag tctcatcaaa
     8341 ctgaccagtc tcgccagtct ctttccagag gagataagcc aactgcaaag gatagcaaag
     8401 tgaatccacc tcatacttgc gctcccagat ccagccgtta aggtctgtgt ggtcagtctc
     8461 gtggtgccct ttccagttct tctcaatgtt gaaggagtta gcatagggat ccttgagtac
     8521 caaggtcatc tgacgtttga ccaaacctgc aatggtctga cgcaggaggg catctctttt
     8581 agctacatga aggtagggtc tgagttgggc tgtcgaatct cgaagccaca tggcaggaat
     8641 atccccagtc aagacaaaag ttgaaccatc ttctaagatt tcaaccgtat tgtccaaggt
     8701 atcggtgtag caacgctcga aaacatccac ccactctgga tggtccttag cccgctctgc
     8761 tacttcatct agccattctc taacaatttc tttcgaataa atcatcgtgc aattccctct
     8821 ttcaaacaag tttcatgttc agtatatagc ttttgagaca gaaaatacat acacaaatga
     8881 tagtcatttt tctacaaaat agttattttt gaaactcctt ttctaaaggc cctctttttc
     8941 tgatataatg tagaaaaaca gctaaaggaa agactatgaa accactactt gaaaccatcg
     9001 atacccgctt tggaactacc aacaagcatg ccttttctcg gggaaatacc ctgccataca
     9061 caggcgtgcc ttttgggatg aattactttg tgccccagac cagtgaccag gatggatctt
     9121 ggttttttga tccgcatctg cctatctttc aggggattcg attaactcac cagcccagtc
     9181 cttggattgg ggactactct tggctccttc tgacacctgt caccagtcaa ctgggtggag
     9241 acagcctctt tcaccgccag tcttcttatg atatagataa agcttgcttc caacctcact
     9301 atttgaagct cttttctctg cgttatcaga ttgaaaccca gctcacaccg acttgctatg
     9361 gtgcttctat tcgtttgaag caaaagcaag gcaaagccct ctccctctat cttcacgcag
     9421 cagatgaact gaccgtagag caagtagata agcggactct tgccctgcga caagaaggta
     9481 aaactgaaac taacaaaaat tcgctaacga tgttcactgc cctgcaaatg aacacggata
     9541 ttcttgctat cagccaagaa gctggggact ggcgaattga cttagcaagt agtcaaaccg
     9601 agatgcaact agccacttct ttcatctctc cttctcaagc tctaatcaat ctacctcaag
     9661 aagattttga tagctgtaaa tcaagtgccc aagtggattg ggaaaatctc ctccatcgtt
     9721 ttgacattat agagacagga gaggctgacc gaaccttctt tgaccactgc ctctacagac
     9781 tcttcctctt cccacagact ttttatgaga ttaatgaatc agggcaagcc atccacatgg
     9841 atctggctac tggtactgtc aagcctggtg tcctctttag caacaatggt ttctgggata
     9901 ccttccgcac caccttcccc ctctttgccc ttatcatacc ggagcactat caacgctttt
     9961 tagaaggttt cctcaatagc taccgcgata ctggtttcct tccaaaatgg ctggctccag
    10021 atgaacgggg tatgatgcca ggtacacttt tagacggtat tatcgcagat agcacctgca
    10081 aggacatggc ccccgaccta gaaggagaac tcttccaagc catgctcgaa acagccagca
    10141 aagccgaccc tctcggaatc aatggccgcc acggactagc ccaataccaa gaactaggtt
    10201 acctctctac cgaccaccat gaaagtgtta gccatactct agactatgcc tatagtgact
    10261 tttgtatcgc cagctgtgcc aaaaaactag agaacataga aatcgctgaa acctacaagg
    10321 ctgcttcaca aaattaccgc cagctatttg acgctgagac aggttacatg cgagcacgag
    10381 acaatcaagg aaactttcac cctgacttct ctccttatag ttgggggcga gactacgctg
    10441 aatgctctgc cattcaagct actttaggcg ttctccacga catccctggc ttaatccaac
    10501 tgatgggtgg aaaagaaacc tttagcaact atcttttgaa agcctgtcaa gatgctcccc
    10561 tctttgaaac aacaggctat ggttacgaaa ttcacgaaat gagcgagatg gctactgctc
    10621 cttttggaca aatagccatt tccaaccagc ctagtttcca cattccttat ctcttccgct
    10681 acagcgatta ccctgactac actgcccttc ttatcaagac gctccgtcag aaagcttttc
    10741 acccaagttg ggaagcctat cctggagatg aagacaatgg tagtctctct gcttggtaca
    10801 tctggtcagc tctcggattt tatccaacct gtccaggaaa accaagctat gacctcggaa
    10861 tccctctctt tgaccatcta cgagtctacc tagctaaaga agataaatgg ctggatatcc
    10921 atactaaaca aaaccacaac cattttaact ttgtcaaaga atgccgactg aacaaaacac
    10981 tcgtatcaac tattcaacac caagacctct taaaagctga acaactaatt ttcaccctca
    11041 gctggctacc aaatcactaa taaccaaaag aacctttgca tcgcgcaaag gttcttcttt
    11101 tatgaaactt ggacttgaag ttgatccaca agctgaccat ccaccgttag gttcagatag
    11161 aaatccaagg ttttatctcc tgcgaaatac aaggttggta gcgtcagact tttttcagtc
    11221 tcaccagctt gaaacgcaag gacttgaatc tcatcctgat aggcgacacc atggacacct
    11281 gtccccggtt gaatcgaaat cttagcttct aaaggaccac tacattctct gcgcttaact
    11341 gtaaaatagg cattttctcc ctttgccaca gctaggcttt tttctgcaaa tgctagcccc
    11401 tgaacagcta ctgtttgtga tagcctcgga gttttataaa gggaaatctt ggtcaataca
    11461 ggaatagatt gtgcctcggt aatcatcacg cgcaccttct gtgcctctac tagcggacct
    11521 cgcaagaggc gcttatgacc aactgtgaag cccctgccaa attcctgcca gacaccatcc
    11581 acctctactt gcacatgaaa agcagcgatt cgttgcccta gcttcaaatc ttctcttaac
    11641 tcaattacat caaaagtttt aggagaacct aagtcgagtt ctaactggat gggcaagtct
    11701 gcatcgcttg cccaagagct ggtctcaagg ccgtctgtca aatggcgaca agcaaagtct
    11761 gcggaaagag ctgaaccaga tacctcaact cccagagcca aatcttcttt atagagctca
    11821 ttgcgatagg tcgcaaattc ataaagtcgt tcaatatcct ttgcatcaaa gagcccagct
    11881 tgattcggcg gaatattaag caagagtgga gttcctcgcc ctactgagtg aaagtagatt
    11941 tcgaccaact cctcgagaga cttaggatcc tgatcctcat ggtagaacca gcctggacgg
    12001 atggaaacat ctgcctctcc gattgaaaaa atcgtgcccg agggatcccc gtgctgaaga
    12061 tagtccagct ctgcttctgt tcctagctta tcaggattca ccttttgcca cagtggatca
    12121 cctgcatacc ctcgttcatt gccaatccag cggatactgg tgccttctgt tgaaaaaatc
    12181 aagcaatcgc cctgcaggtc acgaatggtt tcaaaccatt tttcaaattc ataattaacc
    12241 ttttgcgcgc cctctcctct ggcaccatcc atccaaacct cagcaaattt ccctccattt
    12301 ccataggcag gatttgataa gatttccttc agctgagcca gataataggc attgtagtcc
    12361 gcttctcggt ccacatgata gaggggacta tgggcatccc acggtgacag gtagaccccc
    12421 atatccatat caaattctgt ggcagcttgg gatacttcaa ggagcaagtc gccctctcct
    12481 ctcctccaag gactgacctt aaccgaataa tctgtgtgag ctgtcggata aaggacaaag
    12541 ccatcgtggt gcttgaccac caaaatcaac tttttgaagc ccgtttcctt gagcacacga
    12601 acccactcac gcgcatccaa ctgactcggg ttaaagcgct caggatcctc ctgtccagtc
    12661 ccccattctt ggtcataaaa ggtattagga ccgaagtgga taaaagctgc tagttcatct
    12721 cccagataag ctagctgagt ctggcttggt aacggtccat gcggtttgat tttcttcatc
    12781 gttatatatc tccttctttt gcaacttctc ttgttcacaa ggttatccac aggttgtgga
    12841 taataaacca tgatctaaca gccagctctt tccaactcca actggtaggc tcaggatttt
    12901 tctcctcaat gttccctctt caaacagctc tgtcatatgg ttctgctaac acctctcaga
    12961 atactactct ccataattat tctcaggctt ctttcaggtc attgtgtgaa gaccattcac
    13021 tctccagact tttccacaga ttgtgaataa gtgattattc taaactatca cctcgtcgac
    13081 tataacaaaa aataaaaaga gggtcaataa gataattatc tcttcctaaa tactcagaaa
    13141 agattctata atttgtacaa ttgaacaaga aatgcttctc caaaatctat cttcaaaaga
    13201 atgttgcatc tttttatcct ttcacacaaa catttctctt tatacagatc caagtgataa
    13261 ccagatgata attggacact ttttggtcat ttgtatattt tttgaaaaat tttttaaaaa
    13321 aattttatat cctttttata gaagtttatg catttgtatc tatgtattct atcctatgtt
    13381 atgtataata acataaaatt gtgctaaaca tcataattta tattattttc cgagaaatta
    13441 tagccattga cattacctag gaattgcgtt ataacataca tgtaagcggt acccaaaatg
    13501 gtgccaagtc aaaattttta aggaggaaaa tacatgtctt cacatccaat tcaggtcttc
    13561 tcagaaattg ggaaactgaa aaaagttatg ttgcaccgtc caggcaagga gttagaaaac
    13621 ttgttgccgg actatcttga aaggcttctt tttgatgata ttcctttctt ggaagatgct
    13681 caaaaagaac atgatgcatt tgcccaagct cttcgcgatg aaggaattga ggttctctac
    13741 ctagaacaac tcgctgctga atcattgacc tctccagaaa tccgcgatca atttatcgag
    13801 gaatacttag acgaagccaa catccgtgat cgtcaaacca aggttgctat tcgtgaattg
    13861 cttcacggca tcaaggacaa ccaagaattg gttgaaaaaa caatggctgg gattcaaaaa
    13921 gttgaattgc cagaaattcc tgacgaagct aaagatctaa ctgacttagt tgaatcagag
    13981 tatccatttg caattgaccc gatgccaaac ctctatttca ctcgcgaccc atttgcaaca
    14041 attggaaacg ccgtatcgct taaccacatg tttgcagata ctcgtaaccg tgaaacactc
    14101 tacggtaagt atatcttcaa ataccaccca atctatggcg gaaaagtgga tttggtctac
    14161 aaccgtgaag aagatacgcg tatcgaaggt ggagacgagc tagttctttc taaagacgtc
    14221 cttgcagtag gtatctctca acgtacagac gcagcttcta tcgaaaaact tttggtcaac
    14281 atcttcaaga aaaatgttgg cttcaagaag gttttggcct ttgaatttgc taacaaccgt
    14341 aaattcatgc acttggatac tgtcttcact atggtagact atgacaagtt cactattcac
    14401 ccagaaatcg aaggcgacct tcacgtttac tcagttactt acgaaaacga aaaacttaaa
    14461 atcgttgaag agaaaggtga cttagctgaa cttcttgctc aaaaccttgg tgtagaaaaa
    14521 gttcatttga ttcgttgcgg tggtggcaat atcgtagcag ctgcgcgtga acaatggaac
    14581 gacggttcta acactttgac catcgcacct ggtgtggtag ttgtttatga ccgcaatacc
    14641 gtgaccaata agattttgga agaatacggg cttcgcttga ttaagattcg cggaagtgaa
    14701 ttggttcggg gccgtggtgg acctcgttgt atgtctatgc catttgaacg tgaagaagtg
    14761 taatagctgt tcggcattcg tcaatagaaa atgtaaaaaa tagaaagagg aaataataaa
    14821 atgataaatt cagtattcca aggacgcagc ttcttagcag aaaaagactt tacccgtgca
    14881 gagttagaat accttattgg tctttcagct cacttgaaag atttgaaaaa acgcaatatt
    14941 caacaccact accttgctgg caagaatatc gctctcctat ttgaaaaaac atctactcgt
    15001 actcgtgcag cctttacaac tgcggctatc gaccttggtg ctcacccaga atacctcgga
    15061 gcaaatgata ttcagttggg taaaaaagaa tctactgaag atactgctaa agtattggga
    15121 cgtatgtttg acgggattga attccgcgga ttcagccaac gtatggttga agaattggca
    15181 gaattctcag gcgttccagt atggaacggt ctaactgacg aatggcaccc aactcaaatg
    15241 ctcgctgact acttgactgt tcaagaaaac ttcggtcgct tggaaggctt gacattggta
    15301 tactgtggtg atggacgtaa caacgttgcc aacagcttgc tcgtaacagg tgctatcctt
    15361 ggtgtcaatg ttcacatctt ctcaccaaaa gaactcttcc cagaaaaaga aatcgttgaa
    15421 ttagcagaag gatttgctaa agaaagtggc gcacatgttc tcatcactga agatgctgat
    15481 gaagcagtta aagatgcaga cgttctttac acagacgttt gggtatcaat gggtgaagaa
    15541 gacaaattcg cagaacgtgt agctcttctt aaaccttacc aagtcaatat ggacttagtt
    15601 aaaaaagcag gcaatgaaaa cttgatcttc ctacactgct tgccagcatt ccacgatact
    15661 cacactgttt atggtaaaga cgttgctgaa aaatttggtg tagaagaaat ggaagtaaca
    15721 gacgaagtct tccgcagcaa gtacgctcgc cacttcgatc aagcagaaaa ccgtatgcac
    15781 actatcaaag ctgttatggc tgctacactt ggtaaccttt atattcctaa agtataattt
    15841 tagataataa accgtctacc aacagctatg agggctgcga ctaatagctt tagtccggtc
    15901 ctcttttatg taatggtaat ctattatttc ttataaaata tgtgaaaaat cattaaattg
    15961 aaatctaaac gcattctatt gagtgtgata aaggagaatt tatggcaaat cgtaaaattg
    16021 tagtagcttt gggaggaaat gcgattcttt cttctgaccc atcagcaaag gctcaacaag
    16081 aagctttagt tgaaacagct aagcatcttg taaaattgat taaaaatgga gatgatttga
    16141 ttatcactca cggtaatgga cctcaagttg ggaatctctt gctccaacat ttggcatcag
    16201 actctgaaaa gaaccctgcc ttcccactcg actcacttgt cgctatgaca gaaggtagca
    16261 tcggtttctg gttgaaaaat gctttgcaaa atgctctctt ggatgaaggc atcgaaaaaa
    16321 atgttgcctc tgttgtaacg caagttgtcg tagataaaaa tgatccagct tttgttaact
    16381 tgagtaaacc aatcggtcct ttctattcag aagaagaagc aaaagcagaa gccgaaaaaa
    16441 gcggagcgac tttcaaggaa gatgctggcc gtggctggcg taaggtcgtt gcctcaccaa
    16501 aacctgttga catcaaagaa attgaaacca tccgtactct tttaaataat ggtcaagtcg
    16561 tcgtagctgc aggtggtggc ggtattcccg tcgtcaaaga aaacaatgga catttgactg
    16621 gtgtcgaagc ggttattgat aaagacttcg cttcccaacg tttggcagaa ttggttgatg
    16681 cagacctctt catcgttttg acaggtgtag attatgtatt tgttaactac aacaagccaa
    16741 accaggaaaa attggaacat gtgaatgttg cccagctgga agaatatatc aaacaagatc
    16801 agtttgcacc aggtagcatg cttccaaaag tagaagcagc tatcgctttt gtcaatggtc
    16861 gtccagaagg aaaagcagtt attacttccc ttgaaaatct aggcgccttg attgaatctg
    16921 aaagcggaac aattattgaa aaaggataag ttgttttact aataagatgt attc
//
LOCUS       DAVKLX010000044        15695 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKLX010000044 DAVKLX010000000
VERSION     DAVKLX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15695
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.044"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            186..620
                     /locus_tag="VW325_001617"
     CDS             186..620
                     /locus_tag="VW325_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9734975.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            632..1822
                     /locus_tag="VW325_001618"
     CDS             632..1822
                     /locus_tag="VW325_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU9734976.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1833..2324
                     /locus_tag="VW325_001619"
     CDS             1833..2324
                     /locus_tag="VW325_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9734977.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2339..3118
                     /locus_tag="VW325_001620"
     CDS             2339..3118
                     /locus_tag="VW325_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9734978.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            3105..3910
                     /locus_tag="VW325_001621"
                     /pseudo
     CDS             3105..3910
                     /locus_tag="VW325_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
     gene            3910..4203
                     /locus_tag="VW325_001622"
     CDS             3910..4203
                     /locus_tag="VW325_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9734979.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4225..6126
                     /locus_tag="VW325_001623"
     CDS             4225..6126
                     /locus_tag="VW325_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9734980.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLIDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKGYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6186..7187
                     /locus_tag="VW325_001624"
     CDS             6186..7187
                     /locus_tag="VW325_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9734981.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7297..7632)
                     /locus_tag="VW325_001625"
     CDS             complement(7297..7632)
                     /locus_tag="VW325_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734982.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7643..7885)
                     /locus_tag="VW325_001626"
     CDS             complement(7643..7885)
                     /locus_tag="VW325_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734983.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7901..8095)
                     /locus_tag="VW325_001627"
     CDS             complement(7901..8095)
                     /locus_tag="VW325_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9734984.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWKEDF"
     gene            8261..9211
                     /gene="rsmH"
                     /locus_tag="VW325_001628"
     CDS             8261..9211
                     /gene="rsmH"
                     /locus_tag="VW325_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9734985.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            9223..9540
                     /gene="ftsL"
                     /locus_tag="VW325_001629"
     CDS             9223..9540
                     /gene="ftsL"
                     /locus_tag="VW325_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9734986.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            9544..11796
                     /gene="pbpX"
                     /locus_tag="VW325_001630"
     CDS             9544..11796
                     /gene="pbpX"
                     /locus_tag="VW325_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU9734987.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            11798..12778
                     /locus_tag="VW325_001631"
     CDS             11798..12778
                     /locus_tag="VW325_001631"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9734988.1"
                     /translation="MFISLSAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNMGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            13387..15492
                     /locus_tag="VW325_001632"
     CDS             13387..15492
                     /locus_tag="VW325_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9734989.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLAAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGGLVIREKS"
BASE COUNT         4796 a         2770 c         3551 g         4578 t
ORIGIN      
        1 ttatttataa aaagatttat tttccttaaa aatggaatat tttgaaattt ctgtctatta
       61 gaatgattaa aaagataatg aaagcgctaa tattttttta aaaaaactat tgacaagata
      121 aaaataaagg attacaatgg ggaatataaa gtaaaccggt aaacctaaac aagaaaggag
      181 aaaagatgaa aattgtactt gtagggcatg gacattttgc tacagggatt tatagttctt
      241 tacaattgat tgcaggcaat caagaaaatg tggaggcaat tgactttgtg gaaggaatgt
      301 cagcagatga actcaagcaa aaaatcttac ttgcaatttc aaatgaagaa gaagttttaa
      361 tcctaagtga tctcttggga ggatcgccat tcaaggtttc ttctaccata atgggagaaa
      421 atccagccaa aacaatgaat gttctttcgg gtttgaactt agccatgtta atggaagcag
      481 tctttgctag aatggctcat agctttgatg aggttgttaa taaatcagta gtggcggccc
      541 agggcggagt cgtaaatggt aaagaattgt tttcaacgga tgcagaggaa gaggaagaag
      601 atttcgaatc gggtatttaa agggtaaaag aatgataaaa aaggttacga ttgaaaaaat
      661 aaaatcgcct gagcgcttct tagaagtacc acttctgacg aaagaagaag tcggccaggc
      721 aatcgataag gttattcggc agttagaact caaccttgac tatttcaagg aagatttccc
      781 gacgccagct acctttgata atgtctatcc aatcatggat aacacggaat ggaccaatgg
      841 tttctggaca ggagaactgt ggttggctta tgaatacagt caacaggatg catttaaaaa
      901 catcgctcat aaaaatgttc tttctttcct ggatcgtgtc aataagagag tagaattgga
      961 tcaccatgat ctcggcttct tgtacacacc gtcttgtatg gctgaatata agataaatgg
     1021 agatggagag gctagagaag caaccttgaa agctgcagat aagttgattg aacgctatca
     1081 agaaaaaggt ggttttattc aagcttgggg agacttgggc aagaaagagc attaccgttt
     1141 gattatcgac tgcttgctca atatccaact cttattcttt gcttatcaag aaacaggcga
     1201 tcaaaaatac tacgatattg cagaaagtca tttctatgct tcagctaata atgtaatccg
     1261 tgatgacgct tcgtccttcc acaccttcta ttttgatcct gagacaggtc aaccctttaa
     1321 aggtgtaacg agacaagggt atagtgatga ttcatgctgg gcacgtggtc aatcatgggg
     1381 agtctatggt attcctttga cttatcgtca cttaaaagac gagtcctgct ttgacttgtt
     1441 taagggtgtg accaattatt tcttgaatcg tctgccaaaa gatcatgtgt cctattggga
     1501 tttgattttt aatgatggta gtgctcaatc acgagattct tcagcaacag ctatcgccgt
     1561 ctgtgggatt catgaaatgc taaaacatct cccagaggtg gatgctgaca aagatattta
     1621 taaacatgct atgcatgcca tgcttcgttc cttgatcgaa cattatgcaa atgatcaatt
     1681 tacccctggt gggacaagtc tcctccacgg tgtgtactca tggcattcag gtaaaggagt
     1741 ggatgaaggc aatatctggg gtgactacta ttacctagaa gcccttatcc gtttctacaa
     1801 agactggaac ctatattggt aggaggagaa atatgacaat gccaaatatt attatgaccc
     1861 gtatcgatga acggttgatt catgggcaag gacaactttg ggtaaaatac ctaggttgta
     1921 atacggtcat tgttgccaat gacgaagtaa gcacggacaa gatgcaacaa actctgatga
     1981 aaacagttgt gccagactca gttgccatgc gtttcttccc tttgcaaaag gtgattgata
     2041 tcattcacaa ggctaatcct gctcaaacga tctttatcgt tgtaaaggat gtgaaggatg
     2101 ctttaacctt ggtagaaggt ggtgtcccta tcaaagaaat caatattggg aacattcaca
     2161 atgctactgg taaagagcaa gtgacacgct ccatcttcct gggtgaagag gacaaggcgg
     2221 ccctcaagga attgagccaa actcatcaag taacatttaa tacgaaaaca actccaacag
     2281 gaaatgatgg agctgttcaa gtcaacatta tggactatat ttaaaagagg agatcgttat
     2341 gtcgattaat gtatttcaag cgattttaat tggattatgg acagctttct gttttagtgg
     2401 aatgctgtta ggaatttaca ccaatagatg tattgttctg tcatttggtg tcggaattat
     2461 tctaggtgat ctgcctactg ctcttgcaat gggagctatt ggtgaattgg cttatatggg
     2521 attcggtgtt ggtgctggag gtactgttcc accaaaccca atcggacctg gtatctttgg
     2581 taccttgatg gctatcacta gtgctggtaa agtcagtcca gaagcggctc ttgccctctc
     2641 tactccgatt gctgtggcga ttcaattctt acaaactttc gcctacactg tacgtgctgg
     2701 tgcgcctgaa acagcgatga agcacttgaa aaaccataat ttgaagaaat tcaagttcac
     2761 tctaaatgca acaatttggt tgtttgcctt tattggattt accttgggtt gcttgggtgc
     2821 cctttcaatg gataccttgt tgaaactcgt agactacatt ccaccggtat tgcttacagg
     2881 tttgacagtt gctggtaaaa tgctcccagc tatcggtttt gcgatgatct tgtcagtgat
     2941 ggctaagaaa gagttgattc cctttgtctt gttgggatat gtttgtgcag cttatctaaa
     3001 catcccaaca attggtattg caattgtagg tactatcttt gctttgattg aattttataa
     3061 caagccaaaa acagcggatc atgtggtaga ggaggaagca cacgatgact ggatctaaca
     3121 aattaacaaa acgtgattat cttaaaacgt ctttgcgggc attcttttgt caaaatggat
     3181 ttaactatag taactatcaa gggttgggat atgccaatgt gatgtatcct gctttgaaaa
     3241 aacactatgg agaggatcag gaaggtttct accaagcctt ggaagaaaac tgtgaattct
     3301 ataataccaa cccacacttc ctgcctttta ttaccagctt gcatcttgta atgttggaaa
     3361 atggccgtcc ggcaaaagaa acacgtagca tcaaggacca ttggcaggta ttggggattc
     3421 tctttctcaa ttctgtttag ctccattgtt ctcaaccatc gcagcttcgt ttgctcaaga
     3481 aggcttggtt gtcggtccaa tcttgttctt ccttgcgatg aatacgattt taacagcgat
     3541 taaattgtca actggtctgt atggatacaa actaggaaca actgtgattg ataaactaag
     3601 cgaacagatg gcaacgattt ctcgtattgc caatattatc ggtgtaaccg taattgctgg
     3661 tttggcagcg acatctgtta agattatggt gccgattacc tttgctgcag gggaagttaa
     3721 agcagacgct aaacaaagta tcgtaagtat tcagggaatg cttgataagg ttgctccagc
     3781 tcttctacca gccctattta cacttttagt ttactacttg atcaaagaaa agaaatggac
     3841 aacatataaa ctcgttattt taacagttat catcggaatt atcggaagct ggcttaagat
     3901 tatagcttaa tggatacaac attgttttat ggaatagtga ttgtcttggc agtgagtccc
     3961 cttttactgt caagctttca ttctattcgt cagcaaaagt tgcttcgcaa acagatggag
     4021 caacgacaag agtatttagc ttctttaaca tctggtgatg aagtgttgtt attgtctgga
     4081 attcatggaa aaatcatttc tatccaagat gacttggtct ccttgcagat tgcaaaaggt
     4141 gtggtcatct atgtagaaaa ggaaagtgta atgggaaaga caaaagaact gctttttaag
     4201 tagttctttt tctggtaata gataatgaaa gaagaaagaa gacaattttt tgaaagagtc
     4261 gatggaaacc aatgtcgtga ttatatcttg tcccactgtt caaaagacta tgagaaagtc
     4321 aagagttccc ttgaacgctt gatagacaat cgttttatgt ttgatagtcc ttgggatatg
     4381 gagccttgtt caaaaatcca tcaaatccag ccgatggtat gggatcaagt atttgaagat
     4441 gatccagaat ggtcttatat gctcaatcga caagaatatc tcttgcagtt tatgataggg
     4501 tatctggtag aaggagataa gggctatatc caaaagtgca agttctttct atttgattgg
     4561 attgagcagg tgagagaatt ttctcctcaa tccttgatga ctagaacctt ggatacgggt
     4621 attcgttcct ttacttggtt gaaactactc ttgctcctct tgaaatttga cttgctagag
     4681 gagaaagaac tagagaaaat tttggtcagt ctagaaaagc agattgactt tatgaaaagc
     4741 cactatcgcg ccaagtacac ccttagtaac tgggggattt tacaaacaat tccgatgctt
     4801 gctatctatt atttcttttc agataagatg gacctagaag aagcttacca ttttgcttca
     4861 gaggagttga aacagcaaat tgagacacag attttaggag atggaagcca gtttgaacag
     4921 tcgattctct atcatgtaga ggtttataaa gccttgctgg atttgtgtct cttgcttcca
     4981 gacttgcaag atagttaccg agagttgctg gaaaagatgg cgacctatat tcaaatgatg
     5041 acaggcttag atggacgaac tttggctttt ggtgatagcg attctacaga aacgacagaa
     5101 attttgagcc tgtctgctgt ggttttgaac aaggaagacc ttcttaacgg tctggatgtt
     5161 aaagtcgatt tgcttagcct cttgttcctg gggcgagaaa aggtcaagcg actgcaggaa
     5221 tttgaaagga gagcttggca gcctaagtcc atgatctttg aagactctgg acatgtctgc
     5281 attaaggatg aacatcgtta tctatttttc aaaaatggtc cgctaggaag tgcccatagc
     5341 catagtgacg agaatagttt ttgcttacag tatcaaggcc aacctatttt catagatgct
     5401 gggcgttatt cttatcggga gatatatgaa cgttatctct taaagagtgc ttggagtcat
     5461 tcgacctgca ttgtagatgg gaaagctccg gaaagaatca cgggatcctg ggaatatgaa
     5521 tactatcctc actccctgtt ttgtcaccat aaagaaaggg agggagtgca ttatattgag
     5581 ggggcttatt ggtcagcaga acctgatttg ccttatcttc acaagagaaa aatcctcatg
     5641 ttggtagagg atgtctggct cttggtagat gacatcaggt gtcaaggtca gcatgaggcg
     5701 ttgactcagt ttatccttga caaggatgtg acctatcaag atgggaaaat caatcagttg
     5761 agactatgga gtgaagttga ttttgatttg gaagatacca tcatttctcc taaatacaat
     5821 gagcttgaaa gaagtagcaa actcaccaag cgccaattct ttgagaatca gatgctggat
     5881 tataccatca ttgcgcatga gagttttgaa attatccgtc attctgtcta ccagacagat
     5941 gatcgtgaag tggaaaatgc tctggctttt gaagtgaaaa atgacgaaac agacaagctg
     6001 attctgttat taagcgagga tattggtgta ggtgaaaaat tgtgcctcgt tgacggaaca
     6061 aaaatgcgtg gaaaatgtct agtatatgat aaaataaatg agagaatgat tcgcttgcag
     6121 tgctagaaat aggcattttg aatagtgaat atgttataat aagtattagt aggaggtgtt
     6181 ttagattgga gaagaaactg accataaaag acattgcgga aatggctcag acctcgaaaa
     6241 caaccgtgtc attttaccta aacgggaaat atgaaaaaat gtcccaagag acacgtgaaa
     6301 agattgaaaa agttattcat gaaacaaatt acaaaccgag cattgttgcg cgtagcttaa
     6361 actccaaacg aacaaaatta atcggtgttt tgatcggtga tattaccaac agtttctcaa
     6421 accaaattgt taagggaatt gaggatatcg ccagccagaa tggctaccag gtaatgatag
     6481 gaaatagtaa ttacagccaa gagagtgagg accggtatat tgaaagcatg cttctcttgg
     6541 gagtagacgg ctttattatt cagccgacct ctaatttccg aaaatattct cgtatcatcg
     6601 atgagaaaaa gaagaaaatg gtcttttttg atagtcagct ctatgaacac cggactagct
     6661 gggttaaaac caataactat gatgccgttt atgacatgac ccagtcctgt atcgaaaaag
     6721 gttatgaaca ttttctcttg attacagcgg atacgagtcg tttgagtact cggattgagc
     6781 gggcaagtgg ttttgtggat gctttaacag atgctaatat gcgtcacgcc agtctaacca
     6841 ttgaagataa gcatacgaat ttggaacaaa ttaaggaatt tttacaaaaa gaaatcgatc
     6901 ccgatgaaaa aactctggta tttatcccta actgttgggc cctacctcta gtctttaccg
     6961 ttatcaaaga gttgaattat aacttgccac aagttgggtt gattggtttt gacaatacgg
     7021 agtggacttg cttttcttct ccaagtgttt cgacgctggt tcagccctcc tttgaggaag
     7081 gacaacaggc tacaaagatt ttgattgatc agattgaagg ccgcaatcaa gaagaaaggc
     7141 aacaagtctt ggattgtagt gtgaattgga aagagtcgac tttctaaaat gaaggaaaat
     7201 gacttgcaat ttctgttaag aaataaaata atcccaccta gaacaagcta ggtgggatta
     7261 tttgcctatg aaatgagaaa ttatgggagc aagctcctaa atcaactgtt tttgatctac
     7321 ttctttaact acttgataaa agttatagaa gtaggccaaa cttgaaatga tggttacgac
     7381 taggaatatt gaaaatttcc attggacagg gttggttaaa agttgtggaa aggatatgag
     7441 gagaaagaag agggctgcgt tgaggacagg tatccgtttt gattgtattt tctcaagtcc
     7501 tttattgagc gcaggaagaa agaggagtag gagtagtaaa actgtatgag aaacagctcc
     7561 tgaaataagg gcgaagaaaa ggaaaatact gataaaaaca tgaatgatca gtagtctagc
     7621 tagtgatttc ataaggcacc tcctaatcct ggtctttttt agctcttgca atacgaagtg
     7681 agtcgacaat atgtatcatc actccgaaaa agaaagctcc cagtatagtt ttaaaaatat
     7741 gttttgtatt tagaagagaa ctgataaaat ttggattttc acttgttagg gtatcaatga
     7801 gtggaattat aaaaaatatc actgttccat aaatcgaacc tgctttcaga ccaggataac
     7861 gtaactgttt cttttctttt ttcatgagtt tgctcctttt ttaaaaatct tccttccaaa
     7921 agagactgtt gaggtcagtt tggaggctgc gagcgagatt gagacagagt tccagagttg
     7981 gattgtactt gtcgttttca atcatattga tggtctgtct cgagacaccg atatccttgg
     8041 cgagctcgag ctgggaaatg cccagttcct tgcgaaattc tttcacacga ttcatctggt
     8101 ctcctttctg atttgtgtcg tatatatttg actttattat agtcttttaa acataaagtg
     8161 tcaagtattt ttgacatatt ttttgaagaa atagtagtct ccttgtccta tttgtctgac
     8221 aagtgcaagc tggtcggatt tgtggtaaaa tagataagat atgacaaaag aatttcatca
     8281 tgtaacggtc ttactccacg aaacgattga tatgcttgac gtaaaacctg acggtatcta
     8341 cgttgatgcg actttgggtg gagcaggcca tagcgagtat ttattaagta aattaagtga
     8401 aaaaggccat ctctatgcct ttgaccagga tcagaatgcc attgacaatg cgcaaaaacg
     8461 cttggcacct tacattgaga agggaatggt gacctttatc aaggacaact tccgtcattt
     8521 acaggcacgt ttgcgcgaag ctggtgttca ggaaattgat ggaatttgtt atgacttggg
     8581 agtgtctagt cctcaattag accagcgtga gcgtggtttt tcttataaaa aggatgcgcc
     8641 actggacatg cggatgaatc aggatgctag tctgacagcc tatgaagtgg tgaacaatta
     8701 tgactatcat gacttggttc gtattttctt caagtatgga gaggacaaat tctctaaaca
     8761 gattgcgcgt aagattgagc aagcgcgtga agtgaagccg attgagacaa cgactgagtt
     8821 agcagagatt atcaagttgg tcaaacctgc caaggaactc aagaagaagg gtcatcctgc
     8881 taagcagatt ttccaggcta ttcgaattga agtcaatgat gaactgggag cggcagatga
     8941 gtccatccag caggctatgg atatgttggc tctggatggt agaatttcag tgattacctt
     9001 tcattcctta gaagaccgct tgaccaagca attgttcaag gaagcttcaa cagttgaagt
     9061 tccaaaaggc ttgcctttca tcccagatga tctcaagccc aagatggaat tggtgtcccg
     9121 taagccaatc ttgccaagtg cggaagagtt agaagccaat aaccgctcgc actcagccaa
     9181 gttgcgcgtg gtcagaaaaa ttcacaagta agagggaaaa agatggcaga aaaaatggaa
     9241 aaaacaggtc aaatactaca gatgcaactt aaacggtttt cgcgtgtgga aaaagctttt
     9301 tacttttcca ttgctgtaac cactcttatt gtagccatta gtattatttt tatgcagacc
     9361 aagctcttgc aagtgcagaa tgatttgaca aaaatcaatg cgcagataga ggaaaagaag
     9421 accgaattgg acgatgccaa gcaagaggtc aatgaactat tacgtgcaga acgtttgaaa
     9481 gaaattgcca attcacacga tttgcaatta aacaatgaaa atattagaat agcggagtaa
     9541 gatatgaagt ggacaaaaag agtaatccgt tatgcgacca aaaatcggaa atcgccggct
     9601 gaaaacagac gcagagttgg aaaaagtctg agtttattat ctgtctttgt ttttgccatt
     9661 tttttagtca attttgcggt cattattggg acaggcactc gctttggaac agatttagcg
     9721 aaggaagcta agaaggttca tcaaaccacc cgtacagttc ctgccaaacg tgggactatt
     9781 tatgaccgaa atggagtccc gattgctgag gatgcaacct cctataatgt ctatgcggtc
     9841 attgatgaga actataagtc agcaacgggt aagattcttt acgtagaaaa aacacaattt
     9901 aacaaggttg cagaggtctt tcataagtat ctggacatgg aagaatccta tgtaagagag
     9961 caactctcgc aacctaatct caagcaagtt tcctttggag caaagggaaa tgggattacc
    10021 tatgccaata tgatgtctat caaaaaagaa ttggaagctg cagaggtcaa ggggattgat
    10081 tttacaacca gtcccaatcg tagttaccca aacggacaat ttgcttctag ttttatcggc
    10141 ctagctcaac tccatgaaaa tgaagatgga agcaagagct tgctgggaac ctctggaatg
    10201 gagagttcct tgaacagtat tcttgcaggg acagacggca ttattaccta tgaaaaggat
    10261 cgtctgggta atattgtacc cggaacagaa caagtttccc aacgaacgat ggacggtaag
    10321 gatgtttata caaccatttc cagccccctc cagtccttta tggaaaccca gatggatgct
    10381 tttcaagaga aggtaaaagg aaagtacatg acagcgactt tggtcagtgc taaaacaggg
    10441 gaaattctgg caacaacgca acgaccgacc tttgatgcag atacaaaaga aggcattaca
    10501 gaggactttg tttggcgtga tatcctttac caaagtaact atgagccagg ttccactatg
    10561 aaagtgatga tgttggctgc tgctattgat aataatacct ttccaggagg agaagtcttt
    10621 aatagtagtg agttaaaaat tgcagatgcc acgattcgag attgggacgt taatgaagga
    10681 ttgactggtg gcagaatgat gactttttct caaggttttg cacactcaag taacgttggg
    10741 atgaccctcc ttgagcaaaa gatgggagat gctacctggc ttgattatct taatcgtttt
    10801 aaatttggtg ttccgacccg tttcggtttg acggatgagt atgctggtca gcttcctgcg
    10861 gataatattg tcaacattgc gcaaagctca tttggacaag ggatttcagt gacccagacg
    10921 caaatgattc gtgcctttac agctattgct aatgacggtg tcatgctgga gcctaaattt
    10981 attagtgcca tttatgatcc aaatgatcaa actgctcgga aatctcaaaa agaaattgtg
    11041 ggaaatcctg tttctaaaga tgcagctagt ctaactcgga ctaacatggt tttggtaggg
    11101 acggatccgg tttatggaac catgtataac cacagcacag gcaagccaac tgtaactgtt
    11161 cctgggcaaa atgtagccct caagtctggt acggctcaga ttgctgacga gaaaaatggt
    11221 ggttatctag tcggggtaac cgactatatt ttctcggctg tatcgatgag tccggctgaa
    11281 aatcctgatt ttatcttgta tgtgacggtc caacaacctg aacattattc aggtattcag
    11341 ttgggagaat ttgccaatcc tatcttggag cgggcttcag ctatgaaaga ctctctcaat
    11401 cttcaaacaa cagctaaagc tttggagcaa gtaagtcaac aaagtcctta tcctatgcct
    11461 agtgtcaagg atatttcacc tggtgattta gcagaagaat tgcgtcgcaa tcttgtacaa
    11521 cccatcgttg tgggaacagg aacgaagatt aaaaacagtt ctgctgaaga agggaagaat
    11581 cttgccccga accagcaagt ccttatctta tctgataaag tagaggaagt tccagatatg
    11641 tatggttgga caaaggagac tgctgagacc cttgctaagt ggctcaatat agaacttgaa
    11701 tttcaaggtt cgggctctac tgtgcagaag caagatgttc gtgctaacac agctatcaag
    11761 gacattaaaa aaattacatt aactttagga gactaatatg tttatttccc tcagtgctgg
    11821 aattgtgaca tttttactaa ctttagtagg aattccggcc tttatccaat tttatagaaa
    11881 ggcgcaaatt acaggccagc agatgcatga ggatgtcaaa cagcatcagg caaaagctgg
    11941 gactcctaca atgggaggtt tggttttctt gattacttct gttttggttg ctttcttttt
    12001 cgccctattt agtagccaat tcagcaataa tatgggaatg attttgttca tcttggtctt
    12061 gtatggcttg gtcggatttt tagatgactt tctcaaggtc tttcgtaaaa tcaatgaggg
    12121 gcttaatcct aagcaaaaat tagctcttca gcttctaggt ggagttatct tctatctttt
    12181 ctatgagcgc ggtggcgata tcctgtctgt ctttggttat ccagttcatt tgggattttt
    12241 ctatattttc ttcgctcttt tctggctagt cggtttttca aacgcagtaa acttgacaga
    12301 cggtgttgac ggtttagcta gtatttccgt tgtgattagt ttgtctgcct atggagttat
    12361 tgcctatgtg caaggtcaga tggatattct tctagtgatt cttgccatga ttggtggttt
    12421 gctcggtttc ttcatcttta accataagcc tgccaaggtc tttatgggtg atgtgggaag
    12481 tttggcccta ggtgggatgc tggcagctat ctctatggct ctccaccaag aatggactct
    12541 cttgattatc ggaattgtat atgtttttga aacaacttct gttatgatgc aagtcagtta
    12601 tttcaaactg acaggtggta aacgtatttt ccgtatgacg cctgtacatc accattttga
    12661 gcttggggga ttgtctggta aaggaaatcc ttggagcgag tggaaggttg acttcttctt
    12721 ttggggagtg ggacttctag caagtctcct gaccctagca attttgtatt taatgtaaga
    12781 atggcaccct gatgtttcag ggtgtttttg tatttaaata cacaatgaaa atcaaagaac
    12841 aaactagaaa gctaacttta ggctgctcaa aatatagtag attgaaacta gaatagtaca
    12901 catctgcttc taaaatattg ttagaaatcg atttgactgt cctgatcgat ttgtcctgtt
    12961 cttatttcat tttaatatat agttttgaga tggttgatag aactgacgaa gtcagctcaa
    13021 aacatggttt tgaggttgta gataaggaga agctgatgtg gtttgaagag gttttcgaag
    13081 agtgtaaaaa aatattggtc agttaaagtc aaaactcttg accttttctg accaatgtag
    13141 tatattatga acgtaaggta aatgaaagcc ttactagaac acttatttaa agtaaaatag
    13201 aaagaggtgg gtctatgttt catctagaaa ttttcagaag taaagatagt ctactcctgc
    13261 ttgaaaaaga aaaaccagaa atagtacgta gggtagcggt ttagctagtc taaatttttt
    13321 aaaacaatgg tcaaagatag tcaatatcag tagtcataac taagtaaaca aaaagaggta
    13381 aagaatatga ataacaactt taataatttt aataacatgg atgatttatt taaccaattg
    13441 atgggtggta tgcgaggata tagttctgaa aatcgccgtt acttaattaa tggacgcgaa
    13501 gtcacacctg aggaatttgc tcactatcgt gcgactggtc aattaccagg aaatgcagaa
    13561 actgatgtgc aaatgccaca acaggcatca ggtatgaaac aagacggtgt ccttgcaaaa
    13621 ctaggtcgaa acttggcagc agaagcgcgt gagggcaagt tggatcctgt tatcggacga
    13681 aacaaggaaa ttcaagaaac atctgaaatc ctctcacgtc gcaccaagaa caatcctgtt
    13741 ttggtcggag atgcaggtgt tggtaagaca gcagttgtcg aaggtctagc gcaagccatt
    13801 gtgaacggag atgttcctgc tgctatcaag aacaaggaaa ttatttctat tgatatctca
    13861 ggtcttgagg ctggtactca ataccgtggt agctttgaag aaaatgtcca aaacttagtc
    13921 aatgaagtga aagaagcagg gaatattatc ctcttctttg atgaaattca ccaaattctt
    13981 ggtgctggta gcactggtgg agacagtggt tctaaaggac ttgcggatat tctcaagcca
    14041 gctctctctc gtggagaatt gacagtgatt ggggcaacaa ctcaagacga ataccgtaac
    14101 accatcttga agaatgctgc tcttgctcgt cgtttcaacg aagtgaaggt caatgctcct
    14161 tcagcagaga atacttttaa aattcttcaa ggcattcgtg acctctatca acaacaccac
    14221 aatgtcatct tgccagacga agtcttgaaa gcagcggtgg attattctgt tcaatacatt
    14281 cctcaacgta gcttgccaga taaggctatt gaccttgtcg atgtaacggc tgctcacttg
    14341 gcggctcaac atccagtaac agatgtgcat gctgttgaac gagaaatcga aacggaaaaa
    14401 gacaagcaag aaaaagcagt tgaagcagaa gattttgaag cagctctaaa ctataaaaca
    14461 cgcattgcag aattggaaaa gaaaatcgaa aaccacacag aagatatgaa agtgactgca
    14521 agtgtcaacg atgtggctga atctgtggaa cgaatgacag gtatcccagt atcgcaaatg
    14581 ggagcttcag atatcgaacg tttgaaagat atggctcatc gcttgcaaga caaggtgatt
    14641 ggtcaagata aggccgtaga agctgtagct cgtgctatcc gtcgtaaccg tgctggtttt
    14701 gatgaaggaa atcgcccaat cggcagcttc ctctttgtag ggtctactgg ggttggtaag
    14761 acggagcttg ctaagcaatt ggcactcgat atgtttggaa cccaggatgc gattatccgt
    14821 ttggatatgt ctgaatacag tgaccgcaca gctgtttcta agctaattgg tacaacagca
    14881 ggctatgtgg gttatgatga caatagcaat accttaacag aacgtgttcg tcgcaatcca
    14941 tactctatca ttctcttgga tgaaattgaa aaggctgacc ctcaagttat tacccttctc
    15001 ctccaagttc tagatgatgg tcgtttgaca gatggtcaag gaaatacagt aaacttcaag
    15061 aacactgtca ttattgcgac ctcaaatgct ggatttggct atgaagccaa cttgacagaa
    15121 gatgcggata aaccagaatt gatggaccgt ttgaaaccct tcttccgtcc agaattcctc
    15181 aaccgcttta atgcagtcat cgaattctca cacttgacta aggaagacct ttctaagatt
    15241 gtagatttga tgttggctga agttaaccaa accttggcta agaaagacat tgacttggta
    15301 gtcagtcaag cggctaaaga ttatatcaca gaagaaggtt acgacgaagt catgggagtt
    15361 cgtcctctcc gtcgagtggt tgaacaagaa attcgtgata aggtgacaga cttccacttg
    15421 gatcatttag atgctaaaca tctggaagca gatatggaag atggtggttt ggtcattcgt
    15481 gaaaaatcct aatactcttc gaaaatcaaa ttcaaaccac gtcaacgtcg ccttgccgta
    15541 ctcaagtaca gcctgcggct agtttcctag tttgctcttt gattttcatt gagtataaga
    15601 tagaattttg aggataaaaa agaaggagcc agctgaaaaa aactggttcc ttttgaggta
    15661 cgacgggcat gtcgtgcagt agaggtgtac tattc
//
LOCUS       DAVKLX010000045        15377 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKLX010000045 DAVKLX010000000
VERSION     DAVKLX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.045"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(218..1186)
                     /locus_tag="VW325_001633"
     CDS             complement(218..1186)
                     /locus_tag="VW325_001633"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9734990.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVAVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1331..2114)
                     /locus_tag="VW325_001634"
                     /pseudo
     CDS             complement(1331..2114)
                     /locus_tag="VW325_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            complement(2139..2636)
                     /locus_tag="VW325_001635"
     CDS             complement(2139..2636)
                     /locus_tag="VW325_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9734991.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     AVVEL"
     gene            complement(2709..4070)
                     /gene="radA"
                     /locus_tag="VW325_001636"
     CDS             complement(2709..4070)
                     /gene="radA"
                     /locus_tag="VW325_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9734992.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4084..4599)
                     /locus_tag="VW325_001637"
     CDS             complement(4084..4599)
                     /locus_tag="VW325_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9734993.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4601..5044)
                     /locus_tag="VW325_001638"
     CDS             complement(4601..5044)
                     /locus_tag="VW325_001638"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9734994.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            5349..5498
                     /locus_tag="VW325_001639"
     CDS             5349..5498
                     /locus_tag="VW325_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734995.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            5640..5819
                     /locus_tag="VW325_001640"
     CDS             5640..5819
                     /locus_tag="VW325_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734996.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(6039..6404)
                     /locus_tag="VW325_001641"
                     /pseudo
     CDS             complement(6039..6404)
                     /locus_tag="VW325_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            6424..6591
                     /locus_tag="VW325_001642"
     CDS             6424..6591
                     /locus_tag="VW325_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEU9734997.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            complement(6746..6949)
                     /locus_tag="VW325_001643"
     CDS             complement(6746..6949)
                     /locus_tag="VW325_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9734998.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(6972..7163)
                     /locus_tag="VW325_001644"
     CDS             complement(6972..7163)
                     /locus_tag="VW325_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9734999.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            7735..8103
                     /locus_tag="VW325_001645"
     CDS             7735..8103
                     /locus_tag="VW325_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9735000.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            8103..8336
                     /locus_tag="VW325_001646"
     CDS             8103..8336
                     /locus_tag="VW325_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735001.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            8336..8599
                     /locus_tag="VW325_001647"
     CDS             8336..8599
                     /locus_tag="VW325_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9735002.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            8612..8988
                     /locus_tag="VW325_001648"
                     /pseudo
     CDS             8612..8988
                     /locus_tag="VW325_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
     gene            9005..10075
                     /locus_tag="VW325_001649"
     CDS             9005..10075
                     /locus_tag="VW325_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEU9735003.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            10130..11271
                     /locus_tag="VW325_001650"
                     /pseudo
     CDS             10130..11271
                     /locus_tag="VW325_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(11354..11447)
                     /gene="ffs"
                     /locus_tag="VW325_001651"
     ncRNA           complement(11354..11447)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW325_001651"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(11480..11947)
                     /locus_tag="VW325_001652"
     CDS             complement(11480..11947)
                     /locus_tag="VW325_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9735004.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(12148..13434)
                     /locus_tag="VW325_001653"
     CDS             complement(12148..13434)
                     /locus_tag="VW325_001653"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9735005.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(13665..13901)
                     /locus_tag="VW325_001654"
     CDS             complement(13665..13901)
                     /locus_tag="VW325_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9735006.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCHRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            14167..14964
                     /locus_tag="VW325_001655"
                     /pseudo
     CDS             14167..14964
                     /locus_tag="VW325_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14999..>15377)
                     /locus_tag="VW325_001656"
     CDS             complement(14999..>15377)
                     /locus_tag="VW325_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9735007.1"
                     /translation="RGKVSGRRYQRISLVAGLTNGELIAPMTYEEMVTSDFFEAWFQK
                     FLLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAYIKK
                     NLKKVLPSCNTFYEALFSCSCFN"
BASE COUNT         5121 a         3400 c         2534 g         4322 t
ORIGIN      
        1 actggtatac ctactagaat tctccttaga aagcgtcgat tcaagtgcta tcactgttca
       61 aaaatgatgg tcgctgaaac ttctgatgac gtatagtcat atttcttctc tttttattat
      121 atcacagttt taaatctagc tttactagat tcaccgctac tatccattta ttcggaaaaa
      181 agacgaaaaa acctgagaat catctcaggc ttggtcatta aatttttttc tcaatatcga
      241 aaagtggaga aagtggtcgt ttttcatgaa tacgtacgat agcatcccct aggagatgag
      301 cgattgaaat ctgctcaatc ttatcaatca aacgctcttc tggcagatag atggtatcca
      361 aaacaaccaa tttcttaata gctgattttt ggatattgtc cgtagcagga ccagaaagaa
      421 ctgggtgcgt acagcttgca tagactgcaa cagcaccagc ttccgcaaga gcatctgccg
      481 catgacaaat cgttccagcg gtatcaatca tatcatcaat caagatacaa gtcttgcctt
      541 caaccttacc gatgatgttc ataacttcac tagtattcat cttatcaacg ctacgacgtt
      601 tatcaataat agcgatagat gttttcaaaa attctgccaa cttacgagca cgagtcaccc
      661 ctccatggtc cgggctgaca accacatagt cagaaccaac cataccacgc cgctcaaaat
      721 aatctgcaat tagaggagca cccatcaaat gatccacagg aatatcaaag aatccttgaa
      781 tttgcgcagc atgcaagtcg atggtcaata aacgatccac tccagctact tcaagcatat
      841 ttgcgacaag ttttgaagtg attggctcac gcgctctcgc ctttctatcc tgacgtgcat
      901 acccatagta aggcatgaca acattgacag actctgcact cgcacgcttc aaagcatcta
      961 ccataatcaa aatttcaagc agattgtcat ttacaggcga actagttgat tgtaagataa
     1021 agacgtgttt cccacggatt gattcttcaa tgttgacctg aatctctcca tctgaaaatt
     1081 ggcgaacact tgatttcccc aactctatcc caatctcctg cgccacacgt tctgccaatt
     1141 ctttattaga agaaagggca aacagcttta aatcagaaaa agacatgatt tcctccggta
     1201 tatatgtatc gcttgtgctt ttcacaagat tttccatcta ccattgtagc gctttttgca
     1261 ctatttttca atcaaaaata aaagaagggc accatatttg tacccttgca tcattctttt
     1321 gaaaaatatt ctaggtcatc aactcattgt gtttctcaac aaagcaataa gcatgataat
     1381 aaccatagag agcaatagcc gtaaccactg gaatcgctaa aggcaactct gtttccaact
     1441 ccacaaaagg agagttaaac aataagaccc tgtttcttgc caaccttctg tcctttatag
     1501 gctctgtaaa gcaagtaaac acctactaca gctagacctg aaaaagtcca gtgagaggca
     1561 attcctgaga tgatacgctc taaaattcgc gaaatagtaa agtcaaagcc ctctggcaaa
     1621 tccgtacgaa tataaccaat atcctcaatc atttggaatc ccaaaccgga ggcaattcca
     1681 agtaaaaaca aaaattttaa ttttcgcaca ggaatcaaag ccaaaacaaa aacaagtggc
     1741 aataatttca agggttcttc taccaaagga gccacaatag cactttcaaa ggcatttaaa
     1801 aatggactat ctgggaaaag aacccccagt aaatcatgga tataagtatt agcaaaacta
     1861 gacaaccagc ctaaaaggaa catccctccc aataaagaca gaatcaaaac cttctttggc
     1921 aattcccatt ttttcccaat acggaagaga aaataaagag ccggaatcat gtaaaagaga
     1981 gctagaaaga tagaaactcc cattagtcca tattccgcac ctgacctcga accgtccgta
     2041 tagtagatgg tttcatactg taaaccaata catagcaata aaataaaaat aaataaaata
     2101 ctgcttttct tcatacactt tctttctaaa tgaagtattt ataattctac gactgccata
     2161 cttcctgtat caacattgta aatggcacca gagataatga catcgtctgg tattagggga
     2221 gactcgataa gcagttgcat atcctcgcgt acactctctt ctatatcttg gaagggcaag
     2281 aagtcctggt ctgacacatc gacacccaat tcttccttca aatactcctg aaaaggttca
     2341 ttttcaaagg tctgagcacc acagtctgta tgatgcaata ccacaatttc tcttgtcccc
     2401 atttgttgct gggaaataac tagagaacga atcatatcct cagtcactcg accacctgca
     2461 ttccgcaaaa tatgagcatc cccaagtgcc aaacctagag cttgcgcaac gtgcaaacgt
     2521 gagtccatac aggtcacaat ggctactctg gttttaggtt taagtggcag atttaactgc
     2581 ccatgtaggg caacataagc ctgattggct tgcataaact gttcaaaata cgacacgatt
     2641 ccctccttga aaatttgata gtcaaatatt tctcctatct tatcattttt aagagaattt
     2701 gtcacggatt atgcaaagac ctttttcaag acttcctgaa tcgttgtcac gccaatgacc
     2761 tgaatttcct taggcagagt gattcctgtc aaggaattct taggtacata aatcttagta
     2821 aagcccagtt tagcagcttc gttgatgcgt tgctcaatac gattcacgcg ccgaatctct
     2881 cctgtcaagc ccagttctcc gacaaaacat tcctgaggat tagttggctt gtctttgtag
     2941 ctcgaagcaa tagcaactgc aacagccaag tcaatcgcag gttcatccaa tttaacacca
     3001 ccagcagatt tgagataggc atcctgattt tgcaagagaa gccctgcccg tttttccaaa
     3061 acagccataa tcaagctagc acggttaaaa tcaagtcctg tcgtagtacg cttggcattt
     3121 ccaaacatgg tcggtgttac caaagcctga acctccgcca aaatcggacg cgtcccttcc
     3181 atggttacaa cgatggagga accagtcgcc ccatccaaac gctcttctag gaaaacttga
     3241 ctcggattga gtacctcaac caagccgccc gactgcatct caaaaatccc aatctcatta
     3301 gtggaaccaa aacgattttt gaccgctctc aaaatacgaa aggtgtggtg acgctcccct
     3361 tcaaagtaaa gcaccgtatc caccatatgc tccaacatac gaggcccagc caaggttcct
     3421 tctttggtca catgacctac gataaagatg gcaatgttat tggtcttggc caactgcatg
     3481 agttcagcgg tcacttcacg tacctgagaa acagacccct gcacccctga aatctcagga
     3541 gacatgatgg tctggatgga atcaataatg agaaagtctg gctggatacg ctccacttct
     3601 gcacgaacac tctgcatatt ggtctctgca tagagataaa actcactatc aatatcacct
     3661 aagcgctctg cacgtagttt aatctgctgg gcagactcct ccccactgac atagagaact
     3721 gtccccactt gggacaactg ggttgagact tgtaggagaa gagttgattt cccaatccca
     3781 ggatccccac cgatgaggac gagacttcct ggtaccactc cgcctccaag cacacggttg
     3841 aattcctcca tctccgtctt ggttcgattg acattgatgg aagtcacctc agctagtttc
     3901 atgggcttgg ttttctcacc tgtcaaggac acacgcgcat tcttgacctc ggcaacctca
     3961 acctcttcca caaaagaaga ccaagaccca cagttggggc aacgtcccag atatttaggg
     4021 gaattatacc cacaattttg acatacaaat gtcgcttttt tctttgcgat gacaaacctc
     4081 tttctatatc tctaactcac actcaatcac ttggcaaaaa tcaatcttct catttggcac
     4141 aaactggcgc atgagcattc gatgagcaac aactaccaca gtctgatgtt ctcgatactt
     4201 agacatacat tctagaaacc gagacttcat ttccgtagct gtctcatatt gaataggact
     4261 attaggaagc aactccccct tgttttctaa aaacagtctt ctagctgttt caaagttttc
     4321 tattcctgtt ttatagacct gccattcatg taataaaggc tctactctta aaggaagacc
     4381 cgtagcacag accacatacg aagccgtttc taaagctctt gtgactgcag aagatacgat
     4441 tatttcagct gacgagagta aaggattttt gctcaatttc tggacttgct gccgtcccat
     4501 ctcagacaag ggtgccaaat ctatcccaaa tcctatataa gaacgctcct ctaactcacg
     4561 gtaatctggc tccccatgac gtacaaagat aatcttcatt ctagtgccct gtcgatccaa
     4621 atccaccagt tcgaacgcca tcagctgcat ctccatctgc aattaagaaa gtagcaaaaa
     4681 cagcctggac aatacgttcc ccaacttcaa gaacaacctc ttgatctgtg atattcttca
     4741 tctgcgcaaa aatatgccct tcatttccag gatttccata ataatcccca tcaatgaccc
     4801 caactgagtt aattaaaacc aagcccttct tacgaggatt tgaagaacga tcatagaggt
     4861 agagaacctc agtcggctgc atataagcct taacccctgt cggaaccaag acaatctctc
     4921 ctggcgcaac aactgtacgc acagcaacct ttaagtcgta accagccgca tgcgctgtct
     4981 cacgcttggg caataaattt tcatctgtaa aactcgaaac caattcaaaa ccacgaattt
     5041 tcataatttt ctcttttcta ttatcattta ttctagatta ttctatctta tttattcgga
     5101 aaaagcacga aaaaaagagc acacaacaat tataggcgat acgataattt acactgtttc
     5161 acaatacgtt gaaatttaga gctttaaagc gagggcaaag ttgatttttt acatcgtttt
     5221 acagaggttt acgacacttt tgcccctttt ttgccccttt gtttttcaaa aaaacttcat
     5281 caaatcgctt gactttcacg gtttaccgtg atataatata atcaagataa ggaaaggagg
     5341 tgaggaagtt gaacaaagaa gattggctta ggttactgga aaaggcgata gataatatcc
     5401 ctgaaacggt aactgctata gcaagtctgg tgactgcaat aacggtcgca aggcaaaaca
     5461 aaaagcgtaa accgaaatcc cgtcaaagaa aaaggtgaac gctaagaggt tggggcgaaa
     5521 gcccctcaca cctctatttt atcaaatgaa aagaggaaaa gcaatggtta gtgcaatagc
     5581 tatttttata attgcggtca atgtatatat ttatctaaaa aataaaaagg acaaataaga
     5641 tgagagaagt tattcaagaa ttgttagaca gttcgatgtc tacatctgct atttcccaag
     5701 gtgctggagt tccatggact actgtttctg acctcagaaa aggaaaaaca agcatggaca
     5761 aaatggcact tctcacagca gaaaaacttt atgaatttgc tacaactgat aagcagtgat
     5821 ttcggtcact gtttttattt ttgacaaaac accgtttttg acaataatca aaaaataaaa
     5881 aggagctatt attaacaaaa tggcgttttt ggcaataata ccctgccatt cctcctccct
     5941 attcttcaag aaaacgcttt tttgaagaat cagagcaaat aaaaaaccgc aagcctgagc
     6001 ctgcggtgaa agaacaattt agaaagtttc ctttctattt atttaactgt aatcaagcca
     6061 tctggctcta ctgtgaactc tggcttgtct gccagtgttc cgtctggttt gaggtagtac
     6121 cagcctgttc cgtccgctga ctggataaag gcatttgata ccatggcgcc ttctttagcg
     6181 tctaagtagt accaagtgtc cttgtacttg acccagcctg tcttcatggc accttctaca
     6241 tcaaaatagt accacttgtc agcgattttc ttccagcctg tggccatttc gcctgattgg
     6301 tcaaagtagt accaattacc gtctgtgtgc ttcttccagc ggtctgaaag catatagcct
     6361 gagccatcga aataatacca gataccgttg attttctcaa acttatagaa aggaggagat
     6421 agtatggcaa atactccaat aaaacctgga acagataatc agaaacctgg tcgctatgta
     6481 gaggtaggac ctcgtggcgg aaaagttact aatggtcata ccgcaactat cggaaaaggt
     6541 gatcggctcc ctccgacatc tgctaaaggc aacggttgga aaaaagtcta atcttcgttt
     6601 gcgtacaatc gttcaatggt tgtacgcttt ttccatacac aaaagcacat tcccaaaaaa
     6661 tcaatttgaa tccatgcttc ggcgtaatct ttcccattgc tggcatagtg agttatataa
     6721 tggtgaatca ttttattcct tcctcctatt gagttacttg agaagtatca tgaatggctt
     6781 catagctaag acgcttaaat tcttctgagt ctatctgaaa attgataggc tttttttgta
     6841 aacactcaag aaaactagtg tttcttaaaa gtttttcaac taactcaggg tctgccttta
     6901 caaaggtgga ctctttttgt ccactatacg gatatcgttt tggtctcatt ttcctactcc
     6961 tcaaatcttt cctactcaat cccataatct tcaataacct gaagaatgaa actgttcgct
     7021 cgtggacctt tagtcgttcc acttagaatg tttgtcactt cctgtcgttt aaagccgtaa
     7081 gcaaccgcta gagttgcttt tttaatgcct ttctctttca agaaagcaat aactctttcg
     7141 cgaccgtttg cgatatctgg catattttct cctttctttt tctttcttct ttttctgcta
     7201 taatataagc agaaaggagt taaccttatg acttttaaag aatatttact caaagcaagc
     7261 aaacgcgaca tctacgatga tggtaaagat tttgagaaat caatttcaga ttctcactaa
     7321 tcgagcgtga cttttttgat atggcaatct cagaaattta ccaccagcgc tgactacctt
     7381 aatgactttt tcaaggtggt ctttttcttg ttctaaattt ttaagaatac ttaccatcag
     7441 cacgttcctc ctctttactt atttgtaaat aagaaacaac taaaaatgta actatttttc
     7501 tgcattatac ttgacaactt acaccaaatc ggctaaaatt aaagcataat aaaaacacca
     7561 ataaatctat aaataccgtt cgccaaaaca tttttataat ttatttctta gttgtttttt
     7621 tagttgttta tcacttacaa aaaacatttt acaccttttt ggataattag tcaacctttt
     7681 tacaccaaat ttgttaaaat ttttttgtga tgtcttagaa aggttgattt aacaatgttt
     7741 gagacatttg aaaaaataaa agaattggca aaaaagcgtg gaaaagctct tggacaagta
     7801 gaagaagact taggttatgg tagaaataca ctctataaga taaaaaattc tacgccaaac
     7861 gctgaacgta tcgcagaaat tgctaattat ttcaacgtat ccaccgacta cctgctcgga
     7921 cgaacagata accctgctat cgctgggagt gatgaatttg cccaagtaaa cggacaaatc
     7981 atagacttac gcaaagcagc agccaacacc atgttatttg acgggaaacc actaaatgaa
     8041 gatgatatcg acttcattac atccgtccta tccgcccact tcaaaagcaa aggagaacgc
     8101 taatgactat cactatcaac ttcacagaaa aaaattccta cattacggac tacctaaaca
     8161 aacacggtat cgacacaacg accatggatt ttgacgactt catggcactc atggaagata
     8221 tcgaagacgc acgagcagcc gaccaagcct atatggagta tttagccgac ccagctactt
     8281 ataccatgga tgaggtcttg gatgaactag gactaactcg agaggatatt gcttaatgta
     8341 tcggctagat attgataaaa aagctctcaa gcaacttaaa aaactagata ccccaaccag
     8401 aaaacaaatc ctatcctggc ttgctaaaaa cattgaaaac acgaccaatc cacgacaaca
     8461 cggaaaagca ctaaaagcca accttgcagg ttactggcga tacagagtag agaattaccg
     8521 catcatctgt gatatccaag acgataaact agtcgtccta gccgtggaaa tcgcccaccg
     8581 cagagatgtc tataaataac gaaggagaac tatgacactc gctaaactct gcgaagaata
     8641 tcaagtagaa ctttgtctct tcgacggttc aaactggcac aatagcggtt tctacaatcc
     8701 agacacaaac gtgctcgcta ttgaccacaa cttgactcct gaacaacaaa tccaagtcgc
     8761 cctacacgaa cttggacaca aagaccacac acgctcagag caccagaacg cccgtctacg
     8821 ctgtgaaaac gaagctgata tatgatccat catctcgtaa aagacgctct agaaaactta
     8881 gacgacccca cagagtttga ttacctcaaa ttcatgtctt actacaatct aaaaaccatg
     8941 acaaatgaaa tcatggttaa agaagagtat ttagcattag taaattaaaa aaaggagtat
     9001 caatatggaa attgataaag taaaagccga tttaaaacaa gtcggcaaac gtgtagcaga
     9061 cctcagccaa agcattacga atgaagaaca aacaaagaat gccttcatta tgccgttctt
     9121 ccaagcgctt ggatatgata tcttcaatcc acttgaattt gtcccggaat ttactgccga
     9181 cgtgggcatt aaaaaaggcg aaaaagtcga ctatgcgatc attttggatg gcgagcctca
     9241 aatcctaatt gaatgtaaat caattacaga aaaccttact aagcacgact ctcaactatt
     9301 cagatatttc gtaactacta aatcaaaatt cggtatcttg acaaatggta gagaatataa
     9361 attctttact gatttagatg agccaaataa aatggacaca actccattct taacaatcga
     9421 tgtgactgat atcaaagaaa atcaatttac tgaaatcatc aaattccaca aagagaattt
     9481 tgatattgat aatattgttt catcagcttc tgaattgaaa tacctcaaca atcttaaagc
     9541 gttccttact gaaaatatca ctacaccgtc agatagcttc ctcagatatc taacttctga
     9601 aatctatgaa gggcgtgtta ctcaaaatat cttaacgaca ttttctccca ttattgtgaa
     9661 agggtttaat caattcatca cagagcgagt taatgaaaaa ttaagtgctg cacttaatac
     9721 aagcgttgaa accaaagtaa caaccgatat tccaaaagtt gaagctgaag ctgaagagat
     9781 tgtcgaagta actgacgaaa ttatcacaac tcctgctgaa ttagaagttt atactgttgt
     9841 taaaatgctt gctagggatg tagtttctcc agaacgtgta ttttacagag ataaccgtag
     9901 ctatttcaat gttttggttg acgataacat caaaaaatgg gtattacgtt atcgttcaaa
     9961 ttcaaaaaaa agcactatcg aaatccgtga taaaggcatc ttcccggtgt ctacccctct
    10021 cgaagtcgca aattatgcca atgaaatttt agaagtaatc aagaaattct cataataaaa
    10081 aaagccctct ttactatacc cattttatca aaaaagtgag gtaaaatcaa tgtggatgga
    10141 agaattgcca aacggcaaat ataaattttt tgagagatac aaagatccat atactgagaa
    10201 attaaaaaaa gtctcagtaa cgatggagaa aaaaactccc caagcaagaa atcaagctgc
    10261 gattttactt caggaaaaga taaaacaaaa gttaggagaa aaacaacatt ctgtttctaa
    10321 tataactttt gaaaaactat atgaagaatt tgaggagaat tggaaacacg gcgtaaaaaa
    10381 ctcaaccgtc tacgcttcaa agaatgtaaa aaaagagatt ttaaagcaga tagagggcga
    10441 ctacctagtt agaaatttga tagacgttta ttacaaaaag taatagatca actattacaa
    10501 gatgggagat ctcataacta tgtttctaaa atcaaattca agctcaatca gataatgaaa
    10561 tttgctatca gaatgaatta tattgataca aatgaaatgc tatttgttga aacgcctaga
    10621 aaagtaatta catccgacga actcagaaag aaaaatacaa aatacttaga ccaaaaagag
    10681 tttaagttat tcatccaaaa tttaaaagac gaggccctat gtgattatcg aattacaaag
    10741 tatatccgaa tagctaaagt tctttttctt actggcatga ggtatggaga gctggcagcc
    10801 ttaaactaca aggaagatat agatttttct aaaaagacta ttcacatcaa gcatacatac
    10861 gatttcagac aaaaagagag aactacacca aagacaatca agtccgatag ggttataaca
    10921 gcacctcaaa aagtgttaga tattatcaaa gagcaaataa tagagaatgc gacaaatgga
    10981 tttgatacag attttatttt catcaatact ctaggagaac caataacaaa tgccagggtt
    11041 atttgtgcat tgaaaagaca tggtcaaaaa atcggcatag aaaaaaacat aactacacat
    11101 acatttagac attctcacat atccctactt gctgagctgg gcattccctt gactgccatc
    11161 atggacagag tagggcatag tgactcaaag accacactag agatttattc tcacgttact
    11221 caaaaaatgg tatcagacat atctagcaag ttagacaaga taaaatttta aattgtgccc
    11281 ctcgtctgcc cctttttctt atacaagaca aacaaaaacc ccttaaagtg ttgattttaa
    11341 agggttttta aagtgcacga aaaaagagca cacaattcaa atcgcttagg gctgctggat
    11401 tcctcccctg acccgcttca cgcagaactg ttgctccact atttattata tcacattcct
    11461 attcatttta aaagcaaaat tattttttcc gtctatttct aaaaaaatcc tgcatgatag
    11521 ctgcgcattc atcttccaaa attcctattt caacctccac acgatgattg agacgctcat
    11581 ctgtcaagat atcgtacaaa ctcccagcag cgccaaattt ctggttttta gccccataga
    11641 ccacatttgg aatgcgggca agcccaatcg ccccactaca catgacacaa ggttcaatgg
    11701 tcacaaaaag tgtgcaatcc agcaagcgcc agctctcctc actcaagttc gcatcctcta
    11761 tagccataat ttccgcatgc ataaccgctc gctgtaattc ctcacgcgca ttatgcccac
    11821 gaccaatgat ttccccatct ttgacaatca cacaaccaat tggaatttca tcgtgttcaa
    11881 gagcaatctc agcctctctc aaagcctccc tcataaagac ttctttttct tcaagcgtat
    11941 aatacatctc ttttctcttt tcctacttat cgattttatt attatatcat gaatcccaac
    12001 acaaaagaag ccaccgaatg cggtgactct atagggagat tattatgaaa aagaaaagtt
    12061 taggatattt gttacaacaa gttaggaggt cttcttgtaa ctgtctatag tatacccgac
    12121 ctatcttaaa caaatcttaa aaatctctta ggaccaaaca ctttctaaaa tatttgtttg
    12181 ttcacgacca ggacctactg agaaagtaga aatacgaacg ccaaccaatt cactcacacg
    12241 acgaacatag ttacgcgcat tctcaggaag atcttccaaa ttgcgaactc cggtaatatc
    12301 ttctgaccaa cctggcaact cttcatagat aggcttgcaa cgtttcaatt gctcaagact
    12361 agctggatag tagtcaatac gttgaccgtc aagatcatag gccacacaga ttttcacagt
    12421 atccaaaccg ctcaaaacat caatagagtt caaagaaagg ttagtaatac cagaaacacg
    12481 acggctatga cgcatcacaa ctgagtcaaa ccaacctaca cgacgtggac gaccagttgt
    12541 tgtaccatac tcatgaccca cttcacggat acgttctccc acttcatcaa acaactcagt
    12601 tgggaaagga ccatctccta cacgactcgt ataagcttta catacaccta caaccttgtc
    12661 aatcttgctt ggaccgacac cagaaccaat tgtcacacca ccagctacag ggtttgatga
    12721 cgtaacaaat ggataagtac cttggtcgat atctagcata acaccttgtg caccttcaaa
    12781 aagcacacgt ttgccattat caagcgcatc attcaagata acagatgtat ctatcacgta
    12841 tttcttgatt tgttgaccat attcgtaata ttcttcaaaa atatcatcga aaacaatcgc
    12901 tttactgtca tacaattttt caaaaagacg attcttttca gcaaggttac gttctaaacg
    12961 ctcacggaaa atatctttat ctaaaagatc tgcaatacga attccaacac gagcagcctt
    13021 gtccatataa gctggaccaa ttcccttaat tgtcgtacca atcttattgt cgcccttagc
    13081 ttcttcttgc aagcgatcca actcgatatg ataaggcaaa ataacatgcg cacgatcaga
    13141 aatacgcaag ttatcagttg ttacaccttc ctcatgaaga tagctcaact cttttacaag
    13201 agatttagga tttacaacca taccattccc aatgacagat attttttcag ggaagaaaat
    13261 cccagatgga atcaagtgca acttaaattt cttaccgtca atcacaatcg tgtgaccagc
    13321 attatcacca ccttggtaac gtgcaatcac ttctgcattc gctgaaagga agtctgtaat
    13381 cttcccttta ccttcatcac cccattgggt acctacaaca acaactgaag tcataatttt
    13441 gtctgagccc tcaggctctt cctttctcac atacatggca ggactctcac ctgcaattat
    13501 atcttacaat ttattataat aaaaaatcgc ctttttatca agaagaaaca atagaaagat
    13561 ttgctatttc caactattaa aaaatgattt agaaaaatta ctagctattt actattatct
    13621 ttacataaaa gagtaaatta gttcggaaat ttactaaaat tacctcaaca agaaataaaa
    13681 ccccgattca ttaccaattt ttcaagatac aaacgataag caacacgata atggtaaacg
    13741 ataaaatccc tatgacaacc aatgccatat ctcactaaat aataaattaa aaatttaaaa
    13801 tgaacatgtt cccagtcaaa attatcacca aatgtaggac catactcttc ttcaatacta
    13861 tcatagaaat tagccatctg ctcataaatt ttttgtaaca taatcaaata ctccttttct
    13921 tttttataaa cttattctaa caaaaaattt tacacattca ctatcaattc ctgaattgtt
    13981 aaaataccag cctcaacaag ataaaaatag gaaaagttga caaaatagaa acaaatttgc
    14041 tacctaaatt tcaaacaatt aaagttctaa aaagaccgcc ttaaaacttt tgggtaaatc
    14101 ctattagaac tataatattt ttatataggt attatggttc tgattttcta gtataaaaaa
    14161 attcaaacta gaattcttct tagaaaaggc cgttttaagt gctatcattg ctcaaaaatg
    14221 gagctcgctg agacttctat cgtcaagaag aatcatcaaa ttccttgtat catcaaccaa
    14281 aagattgctc aaaagttaat tgaaaagatt tctatgaccg acattgatca tcagttgtct
    14341 atttcaactt caactgtcat tcgcaagatc aataattttc actttgagca tgatttttcg
    14401 cgtcttcctg agattatgtc ctaggacgtt gaaacagtcc ggggagtgac tgtttcaatc
    14461 gggagatgga gatgagcttt attgctcagg actttgataa actcaatatc ataactgttc
    14521 ttgagagcag aacacaagcc atcatccgaa atcccatgaa tacaaggcta tcaagcgcta
    14581 ctggaagctc attcaacaag atagtcagaa actgagtgat aaacgatttt atcgccctac
    14641 ttttcgtatg cacttgacta ataaagaaat tctagataaa cttttgagct attccgaaga
    14701 cttgaaacac cactataatc tctatcagct cttgcttttc cacctctgtt tcagactttg
    14761 tttaaaacct tcctcaaaga taaagagaaa atcgtcaacg cccttcaatt accttattcc
    14821 aacgccaaac tggaagccac caataatctc atcaaactta tcaagcacaa tgcctttggt
    14881 tttaggaact ttgaaaactt caaaaaagaa aggacgaaat ttgtcctttc tagatcttcg
    14941 ctttcttcaa cccactacag ttgacaaaga accgaaaaat gtctcgcctc taatatagtc
    15001 aattgaaaca agaacaagag aaaagagcct cgtaaaaggt attgcaactt ggtaatacct
    15061 ttttgaggtt ctttttgata taagcccatg ttttctcaat aggattgtac tcaggtgagt
    15121 agggaggaag aggtaaaagt ttatgcccaa actcctcgca taaaagttct aacttaccca
    15181 ttctatggaa tcttgcatta tccataataa taaccgatgg tgtggttaat gttggtaaga
    15241 gaaacttctg aaaccaagct tcaaaaaagt cgctcgtcac catctcttcg taagtcattg
    15301 gagcgattaa ctcaccattt gttagacctg caaccaaaga aatcctctga tatcttcttc
    15361 cagatacttt acctctt
//
LOCUS       DAVKLX010000046        14971 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKLX010000046 DAVKLX010000000
VERSION     DAVKLX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14971
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.046"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            89..1264
                     /gene="mutY"
                     /locus_tag="VW325_001657"
     CDS             89..1264
                     /gene="mutY"
                     /locus_tag="VW325_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9735008.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1320..2024
                     /locus_tag="VW325_001658"
     CDS             1320..2024
                     /locus_tag="VW325_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9735009.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2017..3366
                     /gene="vicK"
                     /locus_tag="VW325_001659"
     CDS             2017..3366
                     /gene="vicK"
                     /locus_tag="VW325_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9735010.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3368..4177
                     /locus_tag="VW325_001660"
     CDS             3368..4177
                     /locus_tag="VW325_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9735011.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4268..4510
                     /locus_tag="VW325_001661"
     CDS             4268..4510
                     /locus_tag="VW325_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9735012.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4512..4766
                     /locus_tag="VW325_001662"
     CDS             4512..4766
                     /locus_tag="VW325_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9735013.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4841..6727
                     /locus_tag="VW325_001663"
     CDS             4841..6727
                     /locus_tag="VW325_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEU9735014.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKGGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFETFSDLVYPQGIYRFSNKLFSDYPNAEKLQGKGTKNKIV
                     SKSLTQLDFVFKNNEEKDHVALLGRIGSERVYRYINKKYLDLPETFENYNVFVPEANG
                     SGALGEVLSTPLIGEPLIGEPLIGHTDTFLSIGNFKTKFEADACIKFIKTKFARVLLG
                     VLKVTQHNSRKTWYYVPLQDFTVNSDIDWTQSVADIDLQLDQKYDLSPEEIAFIENHV
                     REMD"
     gene            6892..10146
                     /locus_tag="VW325_001664"
     CDS             6892..10146
                     /locus_tag="VW325_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9735015.1"
                     /translation="MPVEIKTTKEIHPKIYAYTTPTVTSNEGWIKIGYTERDVTQRIK
                     EQTHTAHIATDVLWTGDAAYTEEPDKGKTFKDHDFHHFLSFHDVERRPKTEWFYFNGT
                     PEKSKNLFDKFVQHDLSGYQPGKGQDYTLRQEQEEAVAKTLAYFQEHAGGKFLWNAKP
                     RFGKTLSTYDLARRMEAVNVLIVTNRPAIANSWYDDFETFIAGQTTYKFVSESDSLKS
                     RPILSRQEFLGILADDVRQLAFISLQDLKGSVYLGGEHDKLKWVTDLHWDLLVIDEAH
                     EGVDTFKTDQAFNKIRRNFTLHLSGTSFKALAKGDFTEEQIYNWSYADEQAAKYSWSL
                     EQEEENPYESLPQLNLFTYQMSQMIGEKLEKGAQIDGENIDYVFDLSEFFATDDKGKF
                     IHEHDVRNWLDTLSSNEKYPFSTKELRNELKHTFWLLERVASAKALKALLEEHPIYEN
                     YEIVLAAGDGRMSEEDDKVKLKSLDLVRKAIAENDKTITLSVGQLTTGVTIPEWTGVL
                     MLSNLKSPALYMQAAFRAQNPYSWSDNKGNHFRKERAYVFDFAPERTLILFDEFANNL
                     SLVTAAGRGTSATREENIRELLNFFPIIAEDRAGKMVEIDAKAVLTTPRQIKAREVLK
                     RGFMSNLLFDNISGIFQASQTVLDILNELPVEKEGKVQDSSDLLDFSDVTVDDEGNAV
                     VDHEIVVNQQMRLFGEKVYGLGESVAELVTKDEERTQKQLVNDLSKTVSSVIVEELKA
                     DYSLKTRETEQIKKQITATLENEIRKNDIERKISEAHIKQELQQQLKEANDKAQKDKI
                     QEDLEKRLEENKLIHKEKLEQTLKKEVEKMPEKFIEQVEIKRVEQLKQSAQDEIRDHL
                     RGFARTIPSFIMAYGDQTLTLDNFDAFVPEHVFYEVTGITIDQFRYLRDGGQDFAGHL
                     FDKATFDEAIQEFLRKKKELADYFKDQKEDIFDYIPPQKTNQIFTPKRVVKRMVDDLE
                     KENPGIFDDPSKTFIDLYMKSGLYIAELVKRLYNSNGLKEAFPNPEERLKHILEKQVY
                     GFAPSEIIYNISTNFIFGNLSKDISRKNFVLADTIPAAKEGSIQKLVDSYFENN"
     gene            complement(10532..11518)
                     /locus_tag="VW325_001665"
     CDS             complement(10532..11518)
                     /locus_tag="VW325_001665"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9735016.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            11712..14180
                     /gene="gyrA"
                     /locus_tag="VW325_001666"
     CDS             11712..14180
                     /gene="gyrA"
                     /locus_tag="VW325_001666"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9735017.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            14180..14923
                     /gene="srtA"
                     /locus_tag="VW325_001667"
     CDS             14180..14923
                     /gene="srtA"
                     /locus_tag="VW325_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9735018.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         4808 a         2533 c         3262 g         4368 t
ORIGIN      
        1 agtagataga aaagttatat actatctatt gaaaatatta taaatcagat acaaagacag
       61 gtgaaacctt ctgtgttata ctagagatat gttagatttg aaagaatacg gtatcgtcat
      121 gtggccggag gagaaagtca tttctttccg tgagaaactt ctcgcttggt atgatgaaaa
      181 caaaagagat ttgccttgga ggagaagtaa aaatccttat cacatctggg tatctgaaat
      241 catgcttcag cagaccaggg tggatacagt tatcccttac tacgaaagat tcctggactg
      301 gtttccaact gtcgaaagtc tggcaactgc gcctgaggag agtttactga aagcttggga
      361 gggcttgggc tattattctc gagttcgcaa tatgcaggct gcagcccagc agattatgac
      421 tgactttggt ggccaatttc caaataccta tgaaggaatt tccagcttga aagggattgg
      481 accttacaca gcaggagcca tttccagtat tgcttttaac ttgcctgagc cagctgtaga
      541 tggtaatgtc atgcgggtct tggcgcgtct gtttgaagtc aaccacgata ttgggattcc
      601 aagtaatcga aaaatttttc aggcaatgat ggagatcttg attaatccgg atcgtccggg
      661 tgattttaat caagcattga tggacttagg gtctgatatt gagtctcctg taaatcccag
      721 acctgaagaa agcccagtca aggactttag tgcggcatat cagaatggaa ccatggaccg
      781 ttatccaatt aaatctccta agaaaaagcc ggttcctatt tatcttaaag ccttagttgt
      841 gaaaaattct caaggacaat ttttacttga aaaaaatgaa agcgaaaagc tcttggcagg
      901 tttttggcat ttccccttta tagaagttga taacttttcg caagaagagc agtttgacct
      961 ctttcatcag gttgcggaag aaagtgtgaa ctttggtccc agtccagaag agagtttcca
     1021 gcaggactat gacctagatg ttgattggct tgatgtttgt tttgatacag tccagcatgt
     1081 ttttagtcat cgaaaatggc atgtacagat tgtagcaggt caggtgagtg acttccatga
     1141 tttttcagat agggaagttc gttggctttc accagaagaa ttcaagaatt acccacttgc
     1201 caaaccccaa caaaaaatct ggcaggctta tgcacaagcc aacttagaca gtagccaaga
     1261 ctagctattg tgtcttctga ctattttttt ggtataatag tagagaaaaa ggtgaacata
     1321 tgaaaaaaat actaattgta gatgatgaga aaccaatctc ggatattatc aagtttaata
     1381 tgaccaagga aggttacgaa gttgtaactg cttttaatgg tcgtgaagcg ctagagcaat
     1441 ttgaagcaga gcaaccagat attattattc tggatttgat gcttccagaa attgatggtt
     1501 tagaagttgc taagaccatt cgtaagacaa gcagtgtgcc cattcttatg ctttcagcca
     1561 aagatagtga atttgataag gttatcggtt tggaacttgg ggcagatgac tatgtaacga
     1621 aacccttctc caatcgtgag ttgcaggcgc gtgttaaagc tcttctgcgt cgttctcaac
     1681 ctatgccagt agatggtcag gaagcagata gtaaacctca acctatccaa attggggatt
     1741 tagaaattgt tccagacgcc tacgtggcta aaaaatatgg cgaagaacta gacttaaccc
     1801 atcgtgaatt tgagcttttg tatcatttag catcgcatac aggtcaagtc atcacgcgcg
     1861 aacacttgct tgagactgtc tggggttatg actattttgg tgatgtccgt acagttgatg
     1921 tgactgtacg acgtctgcgt gagaagattg aagatacgcc cagccgacca gagtatatct
     1981 tgacgcgccg tggtgtaggg tattacatga gaaataatgc ttgatttact gaaacaaacc
     2041 atttttacca gagattttat ctttatcctg attttgttag gtttcatcct tgttgtgacc
     2101 ctcttattac tggaaaatag acgtgataat attcagttga agcaaatcaa tcaaaaggtt
     2161 aaagatttga ttgcaggaga ttattccaag gttcttgata tgcaaggtgg gtctgaaatc
     2221 accaatatta ccaataattt gaatgacttg tcggaggtta ttcgtctcac tcaggaaaat
     2281 ctagaacaag agagtaagag gctaaatagt attctgtttt atatgacaga tggggttctt
     2341 gcgaccaacc gtcggggtca gattatcatg attaacgata cagccaagaa gcaactgggg
     2401 ttggttaagg aagatgttct gaatagaagc attttggaat tgctcaagat agaagaaaac
     2461 tatgaattgc gtgatttgat tacccaaagt ccagaattgt tgctagattc ccaagatatc
     2521 aatggtgaat atttgaacct tcgagttcgc tttgccttga tacgtcgaga gtctggcttt
     2581 atttcaggtt tggtggctgt tttgcatgat acgacggagc aggagaagga agaacgcgaa
     2641 cgaagactct ttgtttccaa tgttagccat gagttacgga ctcctctgac tagcgtaaaa
     2701 tcctatcttg aagccttgga tgagggggct ttgtgtgaaa ctgtagcacc agactttatc
     2761 aaggtttctc ttgatgagac caaccgtatg atgcgcatgg tgacggatct cctccatctt
     2821 tcacgtattg ataatgctac cagtcaccta gatgtggaac tgattaactt cactgctttt
     2881 attaccttta tcctcaatcg ttttgacaag atgaaaggac aggaaaagga gaaaaaatat
     2941 gagttggtga gagattatcc catcaattct atctggatgg aaattgatac agataagatg
     3001 acgcaggttg tcgacaatat tttaaataat gctattaagt attcgccaga tgggggtaaa
     3061 atcactgtca gaatgaagac aactgaagac cagatgattt tatccatttc tgaccacggt
     3121 ttggggattc ctaagcagga tttaccacgt atctttgacc gtttctatcg tgtggatcgt
     3181 gctagaagtc gtgcacaagg tggtacaggt ctaggactgt ctatcgctaa agaaattatt
     3241 aaacaacata agggctttat ttgggccaag agtgaatacg gcaagggttc aacctttacc
     3301 attgtgctcc cttatgataa ggatgcagtg aaagaagaag tatgggagga tgaagtagaa
     3361 gactagaatg agtgaaatag gctttaaata cagtatttta gcgtcgggtt ccagtggaaa
     3421 ttctttttat ctggaaacct caaaaaagaa gcttttagtg gatgcaggct tgtctggcaa
     3481 gaaaattacc agtctgctag ctgaaattaa ccgcaagcca gaagacctgg atgccatctt
     3541 gattacccat gagcattcag atcatatcca tggagtaggc gttttggctc gcaagtatgg
     3601 tatggatctt tatgccaatg aaaagacctg gcaagctatg gaaaatagta aatatcttgg
     3661 caaggtggat tcttcgcaaa agcatatttt tgaaatgggt aaaaccaaaa cttttggaga
     3721 tatcgacatc gagagttttg gtgtaagcca tgatgcagtc gcaccgcagt tctatcgctt
     3781 tatgaaggat gacaagagtt ttgtcctttt gacagataca ggttatgtca gtgaccgtat
     3841 ggcgggcatt gtcgaaaatg cggatggcta tcttatcgag gccaaccatg atgtagagat
     3901 tttgcgatca ggttcttacg cttggcgact caaacaacga atcctatctg accttggtca
     3961 cctttctaac gaggacggtg ctgaagctat gattcggacg ctaggaaatc gcactaagaa
     4021 gatttacctt gggcatttat ctaaggaaaa caatatcaag gaactggctc atatgaccat
     4081 ggtcaatcag ctggctcaag ctgatctggg agtcggagta gactttaagg tttatgatac
     4141 ctcaccagat accgcaacac cattgacaga gatatagaaa gaacgctgag aaggtgttct
     4201 ttttatattg actgaacacc taaaaagtaa tacaatggtg ttaccattaa aaaagggagc
     4261 acaaaagatg actactatta cattaaaagt ttctgaagct gataaaacat ttatgaaagc
     4321 aatggctaag tttgaaggag tttccctttc ggaacttatt cgcaccaaaa ctcttgaagc
     4381 cctagaagat gaatacgatg ctcgtgtggc agatttagcc tatcaagagt atttagaaga
     4441 cttggaaaaa ggagttgaac ccattacttg ggaagaaatg atgcatgatt taggcttgaa
     4501 ggatgaataa tttgtataaa ttagttccaa caagacgttt tatcaagcaa ttgaaaaaat
     4561 tggaccgtta tacgcagaag ctaattacaa actatttaca aaccaatgtt ttggaagacc
     4621 caagacgaca cggaaaggct ttggttggta atcgcgttgg tcaatggcgc tatagaattg
     4681 gtaattatcg agttatcgta caaattgtag atgatgaatt agtcgttgct actctagaag
     4741 ttggtcatcg gagagatatt tattgaatta cttttttcaa gtaaattttc attctttaaa
     4801 atatgatata atagggctta caatataaga gaggttgctg atgacaatag atattagtga
     4861 agaaagtttg gctaaagaat cggctgactt attaaaaatt cttttaaaag atcgaacgac
     4921 aaagaaatca attgtttggg ctacgcattc ttatgaattg ttaggaaagg gatttgctcc
     4981 aagtgatcgc atcaatccaa gtaaggttac tggaaacttt gccaacttga ttcagcccag
     5041 atctgaaaaa tccaaatatg aacaaaaaga tcggacaaaa ataagggcag aagtgtttac
     5101 accaacgtgg ttggtcgcaa agcaaaatgg atatgtagaa tcgaaattag gctcgttaag
     5161 tcttgaggag tatgtggact tacgatggtt agaggtaact tgtggtgaag caccttatat
     5221 ggtaacgaga tatgataccg taactggtga ggaaattcca ttatctgagc gagttggttt
     5281 tgttgataga aaactgcaac ggattagtcg tgaggtttca gatgaagtca cattttataa
     5341 acttgtaaaa gatgcttatc atgcttcgta tggttatgag tatcaaggag attctctcct
     5401 tttggcgcgt gagaatcttt tggcaacttt tgaggactat tatctagcta agactggaaa
     5461 tcaaccaaca ttagagcaaa agaaagaaat tgcgactatt atctcttaca atgtctttca
     5521 gatggatgga ttgaaaaaaa gtagtcctta ttcagctaaa caaaagcaat ctcaacaatt
     5581 gagtttgttt gcggatgagc tagaagtcca agaagtagaa gaatctaaaa cccatatcaa
     5641 agactggaaa aagaatagaa tgataggttt tgagcgccta tctagcgagg aaagtgaaat
     5701 gaaatttgat gtcgtgatag gaaatcctcc gtatcaggaa acaggagaag ccagagatga
     5761 gccgatatat cactatttta ttgatgaagc ttataaaatc gcagataaat ctatactaat
     5821 caccccagca aggtttttat ttaaagctgg tcaaactcct aaaaattgga tggaaaagat
     5881 gcttgaagac aagcatttaa aagttcaatt ctatgaactt aaaagcggta aagtatttac
     5941 aggaacggat attaagggtg gagtagcaat aacttatcgt gatgttgata aagatttggg
     6001 accaatcgga gtatttacta tttttgaaac tttgaatggt attgtaaaaa aaatcgaaaa
     6061 acatccagaa tttgaaactt tttctgattt agtttatcct caaggtattt atagattttc
     6121 aaataaactt ttttctgatt atccaaatgc tgaaaagctg caaggaaaag ggacaaaaaa
     6181 taaaattgtt tcgaaatcac taactcagtt agattttgtt ttcaaaaaca acgaagaaaa
     6241 agaccatgtt gctctacttg gaagaattgg ctccgaacgt gtttatcgat atattaataa
     6301 aaaatattta gatttaccgg aaacattcga aaattataat gtttttgtac cagaagctaa
     6361 tggaagtggt gccttaggtg aagtcttatc aacaccccta atcggggaac ccctaatcgg
     6421 ggaaccccta atcgggcata cagatacttt tttatctatt ggtaatttta aaacaaaatt
     6481 tgaagccgat gcttgtatta aatttattaa aactaaattc gctagagtat tattaggtgt
     6541 tttgaaagtt actcagcata attcacgcaa aacttggtat tacgtccccc tccaagactt
     6601 tacggtcaat tcggacattg attggacaca atcagtggct gatattgacc tccagcttga
     6661 tcaaaaatat gacctttccc ctgaagaaat tgcctttatt gagaatcatg taagggagat
     6721 ggattagaaa agtattttta tttgacaaat agtgctcaat gatctaaaat gactatatag
     6781 gattaggtca ggaagcatac gatgccctga ccctttttgt acttatgaga tgagaaagtc
     6841 atttgttaga taaattgact tcgttagcaa acgttcaaaa aaggaaaact tatgccagta
     6901 gaaattaaaa ccactaaaga gattcatcct aaaatctatg cctataccac accgacagta
     6961 accagtaatg aaggctggat taagattggg tatacagaac gtgatgtcac acaacgtatc
     7021 aaggagcaaa cgcatacagc tcatatagct acagatgtct tatggactgg tgatgcagct
     7081 tatacagaag agcctgataa ggggaaaact ttcaaggacc atgatttcca ccatttcctt
     7141 tctttccatg atgtagaacg tcgtcccaag acggaatggt tctattttaa tggaactcct
     7201 gaaaaatcaa aaaatctttt tgataagttt gttcagcatg atttgtctgg ttatcagcct
     7261 ggaaaaggac aggactatac tctgcgacaa gagcaagaag aagcagttgc taagacatta
     7321 gcttatttcc aagaacatgc tggaggcaag tttctctgga atgccaagcc acgctttggt
     7381 aaaaccttgt ctacctatga cctagctcga cggatggaag ctgtcaatgt cctaattgta
     7441 acaaaccgcc ctgccattgc taactcatgg tatgatgatt ttgaaacatt catagcaggt
     7501 caaacgactt acaagtttgt ttctgaatca gatagcctta agagtcgtcc aatcttgtca
     7561 cgacaagaat ttcttggtat tttagctgac gatgtaagac aacttgcttt tatcagtctc
     7621 caagacttga aaggatctgt ttatttaggt ggagagcacg ataaactcaa atgggtaact
     7681 gatctgcatt gggacttgtt ggttattgac gaggctcatg aaggagttga taccttcaag
     7741 actgaccaag cctttaataa gattcgacgg aattttactc tgcatttgtc aggtacatca
     7801 tttaaagcat tggctaaagg agattttaca gaggaacaaa tctacaactg gtcttatgct
     7861 gatgagcagg ctgctaagta ttcgtggtct cttgagcaag aagaggaaaa tccttatgaa
     7921 agcttgcctc agttgaatct ctttacctat caaatgtctc agatgattgg cgaaaagtta
     7981 gaaaaaggcg ctcagatcga tggtgaaaat attgactatg tttttgactt aagtgaattt
     8041 ttcgctacag atgataaagg gaaatttatt catgagcatg atgtcagaaa ttggttagat
     8101 actctatcaa gcaatgaaaa atatccattt tcaaccaaag aactccgtaa tgaactcaag
     8161 catacttttt ggcttttaga acgtgtcgct tcggccaaag cattaaaagc cctactagaa
     8221 gaacacccaa tctatgaaaa ctatgagatc gttctagctg ctggtgacgg acgtatgtcc
     8281 gaagaagacg ataaagtcaa actcaaatcc ttggacttgg ttagaaaagc gatagcagag
     8341 aatgacaaaa ccattaccct atccgttggt cagctgacga caggtgtcac tatccctgaa
     8401 tggacaggtg tattgatgtt atcaaatttg aaatcaccag ctctttatat gcaggccgcc
     8461 ttccgtgctc aaaatcctta ctcatggagc gataacaaag gaaatcactt tcgcaaagaa
     8521 agagcctatg tatttgactt tgcgccggaa agaaccttga ttctctttga tgagtttgcc
     8581 aacaacttat cgcttgtaac tgcagctggt agaggaactt cagctacacg cgaagaaaat
     8641 attagagaat tattaaactt ctttccaatt attgccgaag accgtgctgg taagatggtt
     8701 gaaattgatg caaaggcagt tctaaccact cctcgccaga taaaagctag agaagttctt
     8761 aaacgaggtt ttatgtccaa tctcttattt gataatatta gtggtatttt ccaagcaagt
     8821 caaacagttt tagatatttt aaatgagctg ccagttgaaa aggaagggaa ggtacaagat
     8881 agttctgatt tattagattt ttcagatgtt acagtcgatg atgagggaaa tgcagtagta
     8941 gaccatgaaa ttgtagttaa tcagcaaatg cgactttttg gtgaaaaagt ttatggactt
     9001 ggtgaatctg ttgctgagtt agtcacaaaa gatgaggaac gaactcaaaa acagctggtc
     9061 aatgacttga gtaagaccgt ttcttcagtg attgtagagg aattgaaagc agattattct
     9121 ctaaaaacaa gggaaactga gcaaattaag aaacaaatta cagcaacact tgagaatgaa
     9181 attcgaaaaa atgatatcga aagaaaaatt tctgaagctc atatcaagca agagttgcaa
     9241 cagcagctca aagaagcaaa tgataaagcg caaaaagata agattcaaga agatttagaa
     9301 aaacgtttag aagaaaataa actcattcat aaagaaaaac tagaacaaac actcaaaaaa
     9361 gaagtggaaa aaatgcctga gaaatttatc gaacaggttg agataaaacg tgtggaacag
     9421 ttgaaacaat cagctcaaga tgaaattcgt gaccatttac gagggtttgc aagaacaatt
     9481 ccaagtttta ttatggctta cggtgatcaa actctaacac ttgataattt tgatgccttt
     9541 gttcctgaac atgtttttta tgaagtaaca gggattacga ttgatcagtt tagatatttg
     9601 cgagatggtg ggcaggattt tgcagggcat ctctttgata aagcaacatt tgacgaagct
     9661 attcaagaat ttcttcgcaa gaaaaaggag ttggcggatt attttaaaga tcaaaaagaa
     9721 gacatttttg actatattcc accgcagaag accaaccaaa ttttcactcc taaacgagtg
     9781 gtgaaaagga tggtagatga tttggaaaag gaaaatccag ggatttttga tgatccatct
     9841 aagactttta ttgatttata tatgaagtca ggcctctata ttgcagaact tgtgaagcgg
     9901 ttatataata gcaatggctt gaaagaggcc tttccaaatc ctgaagaacg cttaaaacat
     9961 attttggaaa agcaagttta tggatttgct ccgtctgaga ttatctataa catttccact
    10021 aattttatat ttggtaatct ttctaaagat atcagtagga agaattttgt tttagcagat
    10081 accattccag cggctaaaga agggagcatt caaaagttgg ttgattccta ttttgaaaat
    10141 aattaaaaag aaggccgagt caaaattctt tgaaatcaga aaaaacgcat aatattgagt
    10201 gcttttgtac tgcccccaaa aagttagaca gaaaaaatct aacttttggg gggcagttca
    10261 gacaatcctt ggtattatgc gttttattgt gggaagatgt ataatggatt gaaataagat
    10321 atgaacaaat cgattaggaa aggaaaattg atttatagaa atgttttagt agtcgatgcg
    10381 tactgttata gattcaacga actataaaat ctgaagaaag caattttaac cagatcttag
    10441 aagcagttgt gtactactct aacttcaata gactatatca tatagataaa aaacaactcc
    10501 ctgatgattc aaggagttgt ctatagttaa attagttttt agaagcttct tggaattctg
    10561 gatttttcca tgcttcgtca atgatagctt gcaattcttt agcagatgct tgcatttttt
    10621 gagtttctgc atcgttcaat ggaatgttta ctggacgaac gataccatgt gcaccaacaa
    10681 cagctggttg accgataaag acattctcaa ctccgtattg accttcttgg aatactgaaa
    10741 gtggaagtac tgcgttttca tcgtcaagga ttgctttagt gatacgagca agggctactg
    10801 cgataccgta gtatgttgca ccttttttgt tgatgattgt gtaggctgca tcacgaacac
    10861 cttcgaacaa ttcaatcaat tcagcttctt gaacattttg agtgtcttta aggaattctt
    10921 caaggtttac accagcgatg ttagcgtgtg accaaacagc gaactcagag tcaccgtgtt
    10981 cacccatgat gtaggcgtgc actgaacgag catccacatc caatttttca gcaagtgctt
    11041 gacggaaacg agctgagtca agtgaagtac ctgaaccgat aacgcgttct ttagggaaac
    11101 cagagaattt ccaagttgag taagtcaaaa cgtcaactgg gttagcagca acaaggaaga
    11161 tacctttgaa accagattca acaacttgag ttacgattga tttgttgata gcaaggtttt
    11221 tacctacaag gtcaagacga gtttcacctg gtttttgagg tgcacctgca gtgatcacaa
    11281 caaggtcagc gtctgcacag tcagagtatt gagctgcata gattttttta ggtgaagtga
    11341 aggcaagggc gtgactaagg tcaagcgcat caccaacagc tttttcatgc aattgtggaa
    11401 tttcgataat tccaagctct tgtgcaattc cttggttaac aagtgcaaaa gcgtaagatg
    11461 aacctacagc accatcaccg acaaggataa cttttttgtg ttgtttagtt gaagtcattg
    11521 ttttaaacat ctccttaatt ttattagggg attttcccta gacaacttca ttctatcact
    11581 tttaaaaaac tttgtcacga atatgcctta tagttctcga tgtaaacgtt ttagtggttt
    11641 agaggctgaa atagatggga atttatggta taatgttgtt acttactaat tgtgaaatga
    11701 ggcatttatt aatgcaggat aaaaatttag tgaatgtcaa tctgacaaag gagatgaagg
    11761 caagttttat cgactacgcc atgagtgtta tcgtagcgcg agctcttcct gatgttcgag
    11821 atggcttaaa acctgttcac cgtcgcattc tctacggaat gaatgaattg ggtgtgaccc
    11881 cagacaaacc ccataaaaaa tctgctcgta ttacagggga tgtcatgggt aaatatcacc
    11941 cacacgggga ttcctctatt tatgaagcca tggtccgtat ggctcaatgg tggagctacc
    12001 gttacatgct tgtagatggt catgggaatt ttggttccat ggatggagat agtgctgccg
    12061 ctcaacgtta taccgaggca cgtatgagca agattgctct ggaaatgctt cgtgatatca
    12121 acaaaaatac agttgatttc gttgataact atgatgccaa tgaacgggaa cccttggtct
    12181 tgccagcgcg ttttccaaac cttttggtta atggagcaac tggtatcgcg gttgggatgg
    12241 caaccaatat tccacctcat aatctgggtg aaaccattga tgcagtgaag ttggtcatgg
    12301 ataatcctga agtgactacc aaggacttga tggaagtctt gcctggacca gattttccaa
    12361 ctggtgctct tgtcatgggg aaatcaggta tccataaggc ttatgaaaca ggtaaaggtt
    12421 cgattgtcct acgttctcgt acagagattg aaacgactaa gactggtcgt gagcgtatcg
    12481 ttgtaacaga atttccttac atggtcaata aaaccaaggt gcatgagcat attgttcgct
    12541 tggttcagga aaaacgcatt gagggtatca cagcagtacg tgatgagtca aaccgtgaag
    12601 gtgttcgatt tgttattgaa gtcaagcgcg acgcctcagc caatgttatt ctcaataacc
    12661 tcttcaaaat gacccaaatg caaaccaatt ttggtttcaa tatgctcgct atccaaaatg
    12721 gtataccgaa aattttgtct cttcgtcaga ttttggatgc ttatatcgag caccaaaaag
    12781 aagtggttgt tcgtcgtaca cgttttgata aggaaaaagc ggaagcgcgc gctcatatct
    12841 tagaaggtct cttgattgcg ctagaccata tcgacgaagt gattcgtatc atccgtgcta
    12901 gtgaaacgga tgcggaagct caagctgagt tgatgagcaa gtttaagctt tctgaacgtc
    12961 aaagtcaagc tatccttgat atgcgtcttc gtcgtttgac aggtttggaa cgcgataaga
    13021 ttcaatctga gtatgatgac ctcttggctc tgattgcgga tttagcagat attcttgcta
    13081 agcctgaacg tgtttctcaa attatcaaag acgaattgga tgaagttaaa cgtaaatttt
    13141 ctgataaacg ccgtacagag ttgatggttg gacagatctt gagtctcgag gatgaggact
    13201 tgattgaaga atcggatgtc ttgattaccc tttctaacag aggctacatt aagcgtttgg
    13261 atcaggacga gttcactgct caaaaacgtg ggggtcgtgg tgtccaagga acgggagtga
    13321 aagatgatga ctttgttcgt gagttagtgt caactagcac ccatgatcat ctgctcttct
    13381 tcacaaacaa gggacgtgtc tatcgtctta aaggttatga aattcctgag tatggtcgga
    13441 ctgccaaagg gctaccagta gtcaatctct tgaaattgga tgaagatgaa agtattcaga
    13501 cggttatcaa tgttgagtct gatcgcagtg atgatgctta tctcttcttt acaacccgtc
    13561 acggtattgt gaagagaact agtgttaagg agtttgccaa tattcgtcaa aatggtctca
    13621 aagcgctgaa tttaaaggat gaagatgagt taatcaatgt cttgttgaca gaaggagata
    13681 tggatattat cattggtacc aagtttggtt atgcagttcg ctttaatcaa tcagccgttc
    13741 gtggtatgag ccgtatcgcc actggtgtga aaggtgttaa ccttcgtgaa ggagacacag
    13801 ttgttggtgc cagcttgatt actgatcaag atgaggttct tattatcaca gaaaaaggat
    13861 atggtaagcg tacagtcgct actgaatacc caacaaaagg tcgtggtggt aagggaatgc
    13921 agacagctaa aattaccgaa aaaaatggct tgctggccgg tcttatgact gttcaaggtg
    13981 atgaggattt gatgattatc actgatacag gtgtcatgat tcgaaccaat cttgccaata
    14041 tttcacaaac aggacgtgca actatgggag ttaaagtaat gcgcctggat caagatgctc
    14101 agatagtgac tttcacaacg gttgcggtgg cagaaaaaga agaagttggg acagaaaacg
    14161 aaacagaagg tgaagcataa tgtctcaaaa aaataataaa aagaaaaaca agcgaaaaaa
    14221 tctgctgaca aatatcctag caggatttct gatattactg tcactggctt tgatttttaa
    14281 tactcaaatt cgaaatattt tcatagtctg gaataccaat aagtatcaag ttagccaggt
    14341 atcaaaagaa aaattagaag aaaatcagga tacagaaggc aattttgact ttgattctgt
    14401 caaagctatc tcttcggaag ctgttctaac ttctcaatgg gatgctcaaa aattaccagt
    14461 tattggggga attgcaattc ctgaattgga aatgaatttg ccgattttta aaggacttga
    14521 taatgttaat ctcttctacg gagctggtac aatgaaacgc gagcaagtaa tgggagaagg
    14581 aaattatagt ctagctagtc accatatctt tggtgttgat aatgctaata aaatgttatt
    14641 ttctccttta gataatgcta aaaatggcat gaagatttat ctaaccgata aaaataaagt
    14701 ttatacttat gaaatacgtg aagtcaaacg tgtgacaccg gatcgtgttg atgaagttga
    14761 tgatagagat ggggtcaatg aaatcacatt agtaacctgt gaagaccttg ctgctacaga
    14821 acgtattatt gtcaaaggtg atttgaaaga aacaaaagat tattcacaaa catctgatga
    14881 aatcctaaca gctttcaatc aaccatataa acaattttat taatacaaat cagtgaaatc
    14941 atggatttca ctgatttttt aagattttca t
//
LOCUS       DAVKLX010000047        14866 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKLX010000047 DAVKLX010000000
VERSION     DAVKLX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14866
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.047"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(186..1817)
                     /locus_tag="VW325_001668"
     CDS             complement(186..1817)
                     /locus_tag="VW325_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU9735019.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLHQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2106..7046
                     /locus_tag="VW325_001669"
                     /pseudo
     CDS             2106..7046
                     /locus_tag="VW325_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
     gene            7136..8332
                     /locus_tag="VW325_001670"
     CDS             7136..8332
                     /locus_tag="VW325_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9735020.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8468..8995
                     /locus_tag="VW325_001671"
     CDS             8468..8995
                     /locus_tag="VW325_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9735021.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9072..9428
                     /gene="glnR"
                     /locus_tag="VW325_001672"
     CDS             9072..9428
                     /gene="glnR"
                     /locus_tag="VW325_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9735022.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9465..10811
                     /gene="glnA"
                     /locus_tag="VW325_001673"
     CDS             9465..10811
                     /gene="glnA"
                     /locus_tag="VW325_001673"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9735023.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLELY"
     gene            complement(10907..11026)
                     /locus_tag="VW325_001674"
     CDS             complement(10907..11026)
                     /locus_tag="VW325_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9735024.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11571..12890
                     /locus_tag="VW325_001675"
     CDS             11571..12890
                     /locus_tag="VW325_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9735025.1"
                     /translation="MPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDT
                     EKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDGWLAIS
                     NYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQRII
                     EAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLL
                     EKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYI
                     QRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNS
                     TGLGTLGRLAIYHENKNPYVWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEE
                     KASGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            12947..13744
                     /gene="psrA"
                     /locus_tag="VW325_001676"
                     /pseudo
     CDS             12947..13744
                     /gene="psrA"
                     /locus_tag="VW325_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
     gene            13755..14354
                     /locus_tag="VW325_001677"
     CDS             13755..14354
                     /locus_tag="VW325_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9735026.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVATSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQL"
     gene            complement(14308..>14866)
                     /locus_tag="VW325_001678"
     CDS             complement(14308..>14866)
                     /locus_tag="VW325_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9735027.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIIFKVDLLFQKVSQLFE"
BASE COUNT         4862 a         2589 c         3076 g         4339 t
ORIGIN      
        1 taataacagg ataaatcgat caggacagtc aaatcgattt ctaacaatgt tttagaaata
       61 gaggtgtact attctagttt caatatacta tatatctgca gaatactctt tcagtttcat
      121 tcagcctagt cttgcttagg aaaaagtaaa aagccatcca attatgatgg atagcttctt
      181 ggttcttaga tttgttctgc aaaaaccttt tcagcaaggt tcatagcgtg gtctgataca
      241 cgagtgtagt gtgagatgat gtcgataaag ttgaccccag cttgtgttga acattcgcct
      301 ttgttgaggc gtttgatgtg ggtttttctg agaacacgtt ctatcttatt gattgcttca
      361 tgacgttcta caagactgcg tgctttttca atatcattgt tttccacact atccagagca
      421 tctttgataa agtcactagt ttggcggtaa acttcctcta attctttcaa ggcggcatca
      481 gaaaattcaa catttttccg ttgaagatag tcagtcagat tgagtagagc ctccgtgtgg
      541 tctccaatcc gttccaaatc acgggaggaa tcaaggatat tggtaagcac ttcactttct
      601 ttttggctga gagcttcgct tgaaagggca atgagataac gtgttaattg ctcatcgatg
      661 gtgttaattg cttcttcggt tttatgccct ttttcagcaa ctttttcatc caagtcaatg
      721 atgtacttat aggaaaggtc aaaggcttta gcagcgtagt ttcctaagtg caagagctct
      781 ttcttagcat ttcctagagc gatagatggg gcctgtttga tgaaatgttc atcaagatat
      841 aagggttcgt atttgacaac ctcgtcctct ccaggaataa tcttggttac aaagtaagcc
      901 agagctccga taaatggaaa ttggacaatg gtgttggtaa tattaaaggt tccgtgagca
      961 aaggcgatgg tcatttccgg tgctagattt agcgtagctt caaaccaatg aatcaggaca
     1021 gtaaaaggaa ctagaaaaat aacgcagacg actgttccga taacgttgaa ggcaacatga
     1081 gctcctgcta cccgtttagc tgcaatatta gcccctaaag aggcaatgat ggctgtaatg
     1141 gttgtcccga tattgtcacc aaatagaacc ggcaaagctc cctgtaggtc aattagattg
     1201 ccggcgtaga ggttttgtaa aatcccaatg gtagccgaag aagcttgaat tagcaaggtc
     1261 aagccagtac cgacaaagac acccaaaaca ggatttttac ttagctcaat catatagtcc
     1321 ttaaagacct gtaaatcctt gagtggagcc attgcgccgc tcatgagatt gagggcaaaa
     1381 aagataccac cgacaccaaa gaggatgcgt ccgatattat tgactgtccg attttttgta
     1441 aaaaagagac agacggcacc gataaagagc ataggtaggg catagttacc taatttaaaa
     1501 ccgatgagaa aggatgtgac agttgtccca atattagcac ccatgacaat cccgatagcc
     1561 tgatgtaagg ttaagagacc ggcactgacc aggccgactg tgataactgt tacaccagaa
     1621 ctagactgaa ttagagcagt catcccaata ccaaccagaa ctccaaagaa aggattacta
     1681 gtatatttgt caatgtaaaa acgaaggcga tctccagcag cttgttgtaa accgtctccc
     1741 atggtcttga tactatataa gaatagcccc agaccaccta aaaagtgaaa taaaatttcc
     1801 tgccaattaa tggacatttc tttttcctcc gaaaaataat cgcggaatat ctcctattct
     1861 attttaaagg ataaaagtaa atctaacaag tgttaatgtc aagtttgtaa aagaaaatta
     1921 taattgataa gtgtattatt tttaagagtt tttaacctta ttttagattt gtgaaaacag
     1981 ctattgtttt ttgtctaaaa atgttttagg ataaaaatgt gattatcatg attctagagt
     2041 taaaaaacta cagaatataa agcgctttct tgaaaacaac aaaatcaatc ttttaggagg
     2101 agaaaatgaa gaatccattt tttgaaagac gttgtcgtta cagtattcgt aagttatcag
     2161 taggagcctg ctcgctgatg attggtgctg ttttatttgc tggtccagcc ttggctgaag
     2221 aaactgcagt tcctgaaaat agcggagcta atacagagct tgtttcagga gagagtgagc
     2281 attcgaccaa tgaagctgat aagcagaatg aaggggaaca tactagagaa aacaagctag
     2341 aaaaggcaga aggagtagcg acagcatctg aaactgcaga agcagctagc gcagctaaac
     2401 cagaggaaaa agcaagtgag gtggttgcag aaacaccatc tgcagaagca aaacctaagt
     2461 ctgacaagga aacagaagca aagcccgaag caactaacca aggggatgag tctaagccag
     2521 cagccgaagc taataagact gaaaaagaag tccagccaga tgtccctaaa aatacagaaa
     2581 aaacattaaa accaaaggaa atcaaattta attcttggga agaattgtta aaatgggaac
     2641 caggtgctcg tgaagatgat gctattaacc gcggatctgt tgtcctcgct tcacgtcgga
     2701 caggtcattt agtcaatgaa aaagctagca aggaagcaaa agttcaagcc ttatcaaaca
     2761 ccaattctaa agcaaaagac catgcttctg ttggtggaga agagttcaag gcctatgctt
     2821 ttgactattg gcaatatcta gattcaatgg tcttctggga aggtctcgta ccaactcctg
     2881 acgttattga tgcaggtcac cgtaacgggg ttcctgtata cggtacactc ttcttcaact
     2941 ggtctaatag tattgcagat caagaaagat ttgctgaagc tttgaagcaa gacgcagatg
     3001 gtagcttccc aattgcccgt aaattggtag acatggccaa gtattatggc tatgatggct
     3061 atttcatcaa ccaagaaaca actggagatt tggttaaacc tcttggagaa aagatgcgcc
     3121 agtttatgct ctatagcaag gaatatgctg ctaaggtaaa ccatccaatc aagtattctt
     3181 ggtatgatgc catgacctat aactatggac gttaccatca agatggtttg ggagaataca
     3241 actaccaatt catgcaacca gaaggagata aggttccggc agataacttc tttgctaact
     3301 ttaactggga taaggctaaa aatgattaca ctattgcaac tgccaactgg attggtcgta
     3361 atccttatga tgtatttgca ggtttggaat tgcaacaggg tggttcctac aagacaaagg
     3421 ttaagtggaa tgacatttta gacgaaaatg ggaaattgcg cctttctctt ggtttatttg
     3481 ccccagatac cattacaagt ttaggaaaaa ctggtgaaga ttatcataaa aatgaagata
     3541 tcttctttac aggttatcaa ggagacccca ctggccaaaa accaggtgac aaagattggt
     3601 atggtattgc taacctagtt gcggaccgta cgccagcggt aggtaatact tttactactt
     3661 cttttaatac aggtcatggt aaaaaatggt tcgtagatgg taaggtttct aaggattctg
     3721 agtggaatta tcgttcagta tcaggtgttc ttccaacatg gcgctggtgg cagacttcaa
     3781 caggggaaaa acttcgtgca gaatatgatt ttacagatgc ctataatggc ggaaattccc
     3841 ttaaattctc tggtgatgta gctggtaaga cagatcagga tgtgagactt tattctacta
     3901 agttagaagt aactgagaag accaaacttc gtgttgccca caagggagga aaaggttcta
     3961 aagtttatat ggcattctct acaactccag actacaaatt cgatgatgca gatgcatgga
     4021 aagagctaac cctttctgac aactggacaa atgaagaatt tgatctcagc taactagcag
     4081 gtaaaaccat ctatgcagtc aaactatttt tcgagcatga aggtgctgta aaagattatc
     4141 agttcaacct aggacaatta actatctcgg acaatcacca agagccacaa tcgccgacaa
     4201 gcttttctgt agtgaaacaa tctcttaaaa atgcccaaga agcggaagca gttgtgcaat
     4261 ttaaaggcaa caaggatgca gatttctatg aagtttatga aaaagatgga gacagctgga
     4321 aattactaac tggctcatct tctacaacta tttatctacc aaaagttagc cgctcagcaa
     4381 gtgctcaggg tacaactcaa gaactgaagg ttgtagcagt cggtaaaaat ggagttcgtt
     4441 cagaagctgc aaccacaacc tttgattggg gtatgactgt aaaagatacc agcctaccaa
     4501 aaccactagc tgaaaatatc gttccaggtg caacagttat tgatagtact ttccctaaga
     4561 ctgaaggtgg agaaggtatt gaaggtatgt tgaacggtac cattactagc ttgtcagata
     4621 aatggtcttc agctcagttg agtggtagtg tggatattcg tttgaccaag ccacgtaccg
     4681 ttgttagatg ggtcatggat catgcaggag ctggtggtga gtctgttaac gatggcttga
     4741 tgaacactaa agactttgac ctttattata aagatgcaga tggtgagtgg aagctagcta
     4801 aggaagtccg tggtaacaaa gcacacgtga cagatatcac tcttgataaa ccaatcactg
     4861 ctcaggactg gcgcttgaat gttgtcactt ctgacaatgg aactccatgg aaggctattc
     4921 gtatctataa ctggaaaatg tatgaaaagc ttgatactga gagtgtcaat attccgatgg
     4981 ccaaggctgc agcccgttct ctaggcaata acaaggtaca agttggcttt gcagatgtac
     5041 cggctggagc aactattacc gtttatgata atccaaattc tcaaactccg ctcgcaacct
     5101 tgaagagcga agttggagga gacctagcaa gtgcaccatt ggatttgaca aatcaatctg
     5161 gtcttcttta ttatcgtacc cagttgccag gcaaggaaat tagtaatgtc ctagcagttt
     5221 ccgttccaaa agatgacaga agaatcaagt cagtcagcct agaaacagga cctaagaaaa
     5281 caagctacgc cgaaggggag gatttggacc ttagaggtgg tgttcttcga gttcagtatg
     5341 aaggaggaac tgaggacgaa ctcattcgcc taactcacgc aggtgtatca gtatcaggtt
     5401 ttgatacgca tcataaggga gaacagaatc ttactctcca atatttggga caaccagtaa
     5461 atgctaattt gtcagtgact gtcactggcc aagatgaagc aagtccgaaa actattttgg
     5521 gaattgaagt aagtcaggaa ccgaaaaaag attacctagt tggtgatagc ttagacttgt
     5581 ctgaaggacg ctttgcagtg gcttatagca atgacaccat ggaagaacat tcctttactg
     5641 atgagggagt tgaaatttct ggttacgatg ctcaaaagac tggtcgtcaa accttgacgc
     5701 ttcgttacca aggtcatgaa gtcaactttg atgttttggt atctccaaaa gcagcattga
     5761 acgatgagta cctcaaacaa aaattagcag aagttgaagc tgctaagaac aaggtggtct
     5821 ataactttgc ttcaccagaa gtaaaagaag ctttcttgaa agcaattgaa gcggccgaac
     5881 aagtgttgaa agaccatgaa actagcaccc aagatcaagt caatgaccga cttaataaat
     5941 tgacagaagc tcataaagct ctgaatggtc aagagaaatt tacggaagaa aagacagagc
     6001 ttgatcgctt aacaggtgag gctcaagaac tcttggctgc caaaccaaac catccttcag
     6061 gttctgccct agctccgctt cttgagaaaa acaaggcctt ggttgaaaaa gtagatttga
     6121 gtccagaaga gcttgcaaca gcgaaacaga gtctaaaata tctggttgct ttattgaaag
     6181 aagacaagtc agcagtcttt tctgatagta aaacaggtgt tgaagtacac ttctcaaata
     6241 aagagaagac tgtcatcaag ggtttgaaag tagagcgtgt tcaagcaagt gctgaagaga
     6301 agaaatactt tgctggagaa gatgctcatg tctttgaaat agaaggtttg gatgaaaaag
     6361 gtcaagatgt tgatctctct tatgcttcta ttgtgaaaat cccaattgaa aaagataaga
     6421 aagttaagaa agtatttttc ttacctgaag gcaaagaggc agtagaattg acttttgaac
     6481 aaacggatag tcatgttatc tttacagcat ctcactttac tcattatgcc tttgtttatg
     6541 aatctgctga aaaaccacaa cctgctaaac cagcaccaca aaacacagtc cttccaaaac
     6601 ctacttatca accggcttct gatcaacaaa aggctcctaa attggaagtt caagaggaaa
     6661 aggttgcctt tcatcgtcaa gagcatgaaa atgctgagat gctagttggg gaacaacgag
     6721 tcatcataca gggacgagat ggactgttaa gacatgtctt tgaagttgat gaaaacggtc
     6781 agcgtcgtct tcgttcaaca gaagtcatcc aagaagcgat tccagaaatt gttgaaattg
     6841 gaacaaaagt aaaaacagta ccagcagtag tagctacaca ggaaaaacca gctcaaaata
     6901 cagcagttaa atcagaagaa gcaagcaaac aattgccaaa tacaggaaca gctgatgcta
     6961 atgaagccct aatagcaggc ttagccagcc ttggtcttgc tagtttagcc ttgaccttga
     7021 gacggaaaag agaagataaa gattaaatat cgaaaaatct tgtgaaatct ttccttatat
     7081 ttccaaagtg tgatataata gtttcgaata aaataaataa aggagttttt gtaacatggc
     7141 aaaacttact gttaaagacg ttgacttgaa aggtaaaaaa gtcctcgttc gtgttgactt
     7201 caacgtacca ttgaaagatg gcgtaatcac taacgataac cgtatcacag cagctcttcc
     7261 aactattaag tacatcatcg aacaaggtgg acgtgcaatt cttttctctc accttggacg
     7321 tgtgaaagaa gaagctgata aagctggtaa atcacttgct cctgtagcag ctgacttggc
     7381 agcaaaactt ggtcaagatg ttgttttccc aggtgtcact cgtggtgctg aattggaagc
     7441 ggcaatcaac gctcttgaag atggacaagt tctcttggtt gaaaacactc gttacgaaga
     7501 tgttgacggc aagaaagaat ctaaaaacga tcctgaactt ggtaaatact gggcatcact
     7561 tggagatggt atcttcgtaa acgatgcatt cggtacagct caccgtgcac acgcatctaa
     7621 cgttggtatc tcagcaaacg ttgaaaaagc agttgctggt ttccttcttg aaaacgaaat
     7681 tgcctacatc caagaagcag ttgaaactcc agaacgtcca ttcgtggcta tccttggtgg
     7741 ttcaaaagtt tcagacaaga tcggtgttat cgaaaacttg cttgaaaaag ctgataaagt
     7801 ccttatcggt ggtgggatga cttacacatt ctacaaagca caaggtatcg aaatcggtaa
     7861 ctcacttgta gaagaagaca aattggatgt tgcgaaagct cttcttgaaa aagcaaatgg
     7921 taaattgatc ttgccagttg actcaaaaga agctaacgca tttgctggtt acactgaagt
     7981 gcgtgacact gaaggtgaag cagtttctga aggcttcctt ggtcttgaca tcggtccaaa
     8041 atctatcgcc aaatttgacg aagctttgac tggtgccaaa acagttgtat ggaacggacc
     8101 tatgggtgta tttgaaaacc cagatttcca agctggtaca atcggtgtga tggacgctat
     8161 cgtgaaacaa ccaggagtta aatcaatcat cggtggtggt gactcagctg ccgcagcgat
     8221 taaccttggc cgtgcagaca agttctcatg gattagtacg ggtggtggag catcaatgga
     8281 acttcttgaa ggtaaggttc ttccaggact tgcagccctg acagaaaaat aagattttat
     8341 aaacaaatca aagaagagag ggatgaaagt tcctcttttc ttttgcttaa aataaaaacg
     8401 cttcctctca actattactc ataaaaatca ccgatttatg ataaaatgga aatagaaagt
     8461 tgagattatg agttatttta aaaaatataa attcgataaa tcccagttca aacttggtat
     8521 gcgaaccttt aaaacaggta ttgctgtttt tctagttctc ttgatttttg gcttttttgg
     8581 ctggaaaggt cttcaaattg gtgctttgac agccgttttt agcctgaggg agagttttga
     8641 tgagagtgtt cattttggga cttcgcgtat tctaggaaat agtatcggtg gactctatgc
     8701 cttggtcttt ttcttattaa ataccttttt ccacgaagcc ttttgggtga ccttggtagt
     8761 tgttccaatc tgcaccatgt taaccattat gacaaatgta gccatgaata acaaagcagg
     8821 ggttattggt ggtgtagcag ctatgttaat cattacccta tcaattccaa gtggtgagac
     8881 aattttgtac gtgtttgtgc gtgtattaga aacgtttatg ggagtttttg tcgcaattat
     8941 cgtaaattac gatattgatc gtattcgtct ctttttagag aaaaaagaaa aataatgtta
     9001 cattttataa cattatcaat tgacgtttgt ctttttttag actataatag acagaaagaa
     9061 ggaaattgta aatgaaggaa aaagaatttc gccgaaatat ggctgttttt cctatcggca
     9121 gtgttatgaa gttgaccgat ttatcggcgc gtcagattcg ttattatgaa gatcaagagt
     9181 tgatcaagcc cgatcgaaac gaaggaaatc gtcgcatgta ttccttgaat gacatggatc
     9241 gtctgcttga aatcaaagat tatatctctg aaggttataa tatcgctgcc attaagaaaa
     9301 aatatgctga acgtgaagcg aaatccaaga aagcggttag tcagactgaa gtacgtcgtg
     9361 cacttcacaa tgaactcctc caacaggggc gttttgcttc agtacagtca ccttttggtc
     9421 gcggttaggc aaccgcaagt agtcatagat taaggagaaa acttatgcca atcacagctg
     9481 cagatattcg tcgtgaagtc aaggaaaaaa atgttacctt tattcgtctt atgttctcag
     9541 atattttggg aaccatgaaa aacgtcgaaa ttcctgctac agatgaacag ttagataagg
     9601 tcttgtcgaa caaggttatg tttgatggat cttctattga aggttttgta cgtatcaatg
     9661 agtcggatat gtacttgtac ccggacttgg atacatggac agtcttccct tggggagatg
     9721 aaaatggaag tgttgcaggt ctgatctgtg atgtctatac aacagaaggt gaaccatttg
     9781 cgggtgaccc tcgtggtaat ttgaaacgag ctcttcgtca catggaagaa gttggattca
     9841 aatccttcaa ccttggtcca gagccagaat tcttcctatt taagttggat gaaaatgggg
     9901 acccaacact tgaagtgaat gacaagggtg gctactttga tttggcacct actgaccttg
     9961 cggacaacac acgtcgtgag attgtgaatg tcttgaccaa aatgggattt gaagtagaag
    10021 cgagtcacca cgaggttgcg gttggacagc atgagattga ctttaagtac gatgaagttc
    10081 tccgtgcttg tgataagatt caaatcttta aacttgttgt taaaaccatt gctcgcaaac
    10141 acggacttta cgcaacattt atggcgaagc caaaatttgg tattgctgga tcaggtatgc
    10201 actgtaatat gtccttgttt gatgcagaag gaaataacgc cttctttgat ccaaatgatc
    10261 caaaaggaat gcagttgtca gaaacagctt accatttcct aggcggtttg atcaagcatg
    10321 cttacaacta tactgccatc atgaacccaa cagttaactc atacaaacgt ttggttccag
    10381 gttatgaagc gcctgtttac attgcttggg ctggtcgtaa ccgttcgcca cttgtgcgcg
    10441 tacctgcttc acgtggtatg ggaactcgtc ttgagttgcg ttcagtggat ccaatggcga
    10501 acccttacgt tgctatggct gttcttttgg aagttggttt gtatggtatt gaaaataaaa
    10561 tcgaagcacc agctcctatc gaagaaaata tctacatcat gacagcagaa gagcgcaagg
    10621 aagctggtat tacagacctt ccatcaactc ttcacaacgc tttgaaagct ttgacagaag
    10681 atgaagtggt taaagctgct ctcggagatc acatctacac tagcttcctt gaagccaaac
    10741 gaatcgaatg ggcaagttat gcaaccttcg tttcacaatg ggaaattgat aattatttag
    10801 agctttacta atactaatat agaagaaaga ttgcctgtgg gtaaacagtc acacagcagt
    10861 ctaagccatc aaatacctat aacccttaaa atagcgattt cggtcgctat ttatttttcg
    10921 aaaaatcctc ttgactcttt tgcgaattag atttatcatc agacgtgtag aagaaaatta
    10981 aggagtcact atggaaaaat tcaatttcaa aaacaatatc gggcaagaaa acaagttact
    11041 tcaaatcgaa atctacaagt ttactaactt ttgcaagctt caaaattaca catctgtaaa
    11101 tattttttcg aaagatatat ttgaaaccat agtaaattga gactagaata gtacactaca
    11161 tctgctaaaa atttctagaa actaatttaa ctttcctaat cgatttgttt acatcttatt
    11221 tcaatctact atattatctc aataaatgat gatggcgact gttttgtttg tgcttgtcga
    11281 tttttgaatc ctgatcgagc ggaagaatat ttctgccgag gttgccaagg ggaaaatcct
    11341 gaagatattg aattttatga tgaacaatta caggcagaaa aggtggagga tttgaatatt
    11401 cgattggaag taaaaaattg aattatctac aatctcatca acaataaacc tatttcaacc
    11461 ccattatcta taggcgtatt tttacgcctt tttatatact tgaagcacat aagaaacatt
    11521 atgggaagtt tgctgatgga agtactcaag aaattgatgt tccttatgat attcctgata
    11581 cttgggagtg ggtgaggttt tctacattgg ttgaaattgt cagaggtggc tctccacgac
    11641 caatcaaaga ttatcttact tctgaagtag atggaataaa ttggataaaa ataggtgata
    11701 ctgaaaaggg tgaaaagtat ataaataatg ttaaagaaaa aatcaaaaaa tcagggctta
    11761 acaaaactag atttgtaaaa aaaggtacat ttttgttaac taattctatg agttttggta
    11821 gaccttatat tttgaatgtt gatggtgcaa tacacgatgg atggttggct atttcgaact
    11881 atgaaaactc attaaataaa gattacctat tctatattct ttcatcaaat gtagtttatt
    11941 ctcaatttct atctctaatt agtggagctg ttgtgaaaaa cttgaatagt gataaagttg
    12001 cttctattct tatccctctc cccccactat ccgaacaaca acgcataata gaagcaatcg
    12061 aatcagcttt agaaaaagta gatgaatatg ctgaaagtta taatagacta gaacagctag
    12121 ataaagaatt tccagataaa ctaaaaaaat ctattcttca atatgctatg caaggaaaat
    12181 tagttgaaca agacccaaat gatgaatcag tcgaagtttt acttgaaaaa atacgagcag
    12241 aaaaacaaaa actctttgaa gaaggcaaga ttaaaaagaa agatttggac atttctattg
    12301 tttcccaagg agatgataac tcttattatg aggaagtacc ttgtgaaata ccagaaagtt
    12361 gggaatgggt gaggttaaac gatataacct cctacattca aagagggaag tctccaaaat
    12421 attcaaatat tcctatatac cctgtgattg ctcagaaatg taatcaatgg tctggctttt
    12481 ctattgactt agcaagattt attgatcccg aaacagttca tagttaccag aaagaaagat
    12541 tactaagaga tggtgattta atgtggaatt caacaggtct aggtacgtta ggacgattag
    12601 ctatttacca tgaaaataaa aatccttatg tctgggctgt tgcagatagc catgttacag
    12661 ttataagagt cttatcggga gttattaatt gccatttcat ctataatttt ctttcctctc
    12721 ctattgttca gtcagttatt gaagaaaaag cgtcgggtag tacaaaacaa aaagaattat
    12781 taacgaaaac tataaaagaa tatttaatcc ccctcccacc ccttcctgaa caatccagaa
    12841 tcgttgataa aatcgaacaa tttttcgccc atattgacgc actaatttag tataatagtt
    12901 ttacgtgcgg tggaatttct atcaaacata agataggagt tttacgttga aagattttga
    12961 attacatttg aagaaagctg gtttagctga aaatactgtg cgttcgtacc tttatggtgt
    13021 tcgatttttc ttggagaatt acgaactcaa aatggaagat ttatttgaat acaaaggata
    13081 tttattagac aattttaaac ctaaaactgt caatttgcga ctacaaggag taaataaata
    13141 tctggctttt attggtcatg atgatttgaa attaaaattt gtcaaagtac agcaaaaacc
    13201 gtttttagaa gatgtcatta gccatgctga ttaccttttt cttaaacgta gtttgaaaaa
    13261 agatggtatt ttaaaatggc attttgtcgt ttgattttta ggtgcaactg gggctcgtgt
    13321 aagtgaactt attaaactaa aagtagagca tgtagaaatc ggttattttg acatttactc
    13381 aaaaggtggt aaaattcgta gactgtacat tcctaaaaaa ttaagaaata gttgtcttag
    13441 ttggttagaa tcagaaaatc gccgaagtgg ttatttattt ttgaataaat ttaacgaacc
    13501 aattacagca agaggagttg ctcaacagtt aaaaaattat gctgataaat acaaaatgaa
    13561 tcctaaagta atttaccctc attcttttag gcatttattt gctaagaatt ttttagcgaa
    13621 gtataatgat attgccttgc ttgcagattt gatgggacac gaaagtatag aaactactcg
    13681 aatttatcta aggaaaacag ctactgaaca acaaaatatt gtagataaaa ttgttaattg
    13741 gtaaaaaata acaggtggtc aaactgacta cctgctattt ttgtgattat ggctcttatt
    13801 atgggaatat acctatgaat tgggttgtta taaaaataaa agatattttt tcaatgaata
    13861 caggtctttc ttacaagaag ggcgatttaa gcattaataa taaaggtgtt agaattatac
    13921 gtggtggtaa tattaagcct ttagaatttt ctctgttgga taatgattac tacattgata
    13981 cacaattcat ctcctctgag caagtttatt taaaacataa tcagctaata acacctgtag
    14041 caacctcttt agaacatatt ggaaagtttg caagaatcga taaagactat gatggtgttg
    14101 tggctggtgg atttattttc caattaacac cattcgaaag ttcagagatt atttcaaaat
    14161 ttctattatt taacttgtcc tctccgttat tttataaaca attgaaagca ataactaaac
    14221 tatcaggtca agctttatat aatattccta aaactacact gagcgagcta ttaattccgt
    14281 tagctccttt tgaggaacag gaacttatta ctcaaaaagt tgagaaactt tttgaaaaag
    14341 taaatcaact ttgaaaatga ttcttttcat ctcttcatga ttagaaatag ggattaataa
    14401 ttcggagata ctggtactat ttaatgtttt ccctttgata gcatcttttg aatcacctaa
    14461 agtagagata agtggcaaaa atatcattaa gtaatctctg ataatatttt ctttattagc
    14521 ataggggaat atcgatataa tggcttcatt atgagtggca ggaatatcca atatggcaac
    14581 ttttccaata gataatttaa aactcattaa taaagttcct ttaggtgaaa tgtctatttt
    14641 ctttgatttt aatgctaatt tagaaataga ttctctcgta ttagttacat aaccagatat
    14701 aggcatatct gatatagata cccaaggtat ttcagttccc caaaaagtag cttcactgcg
    14761 tggaggagtt tttcctattc tgaagttaac taggctagca aatttaatat atctccatgc
    14821 ttctgggatt tcatatatag gataagaggt tgtttcgtct ttgttc
//
LOCUS       DAVKLX010000048        14453 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKLX010000048 DAVKLX010000000
VERSION     DAVKLX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.048"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(122..1972)
                     /locus_tag="VW325_001679"
     CDS             complement(122..1972)
                     /locus_tag="VW325_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9735028.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2014..3171)
                     /locus_tag="VW325_001680"
     CDS             complement(2014..3171)
                     /locus_tag="VW325_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9735029.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNML"
     gene            3229..4395
                     /locus_tag="VW325_001681"
     CDS             3229..4395
                     /locus_tag="VW325_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9735030.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            4534..4860
                     /locus_tag="VW325_001682"
     CDS             4534..4860
                     /locus_tag="VW325_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9735031.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            4847..5440
                     /locus_tag="VW325_001683"
     CDS             4847..5440
                     /locus_tag="VW325_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9735032.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYATRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            5433..6344
                     /locus_tag="VW325_001684"
     CDS             5433..6344
                     /locus_tag="VW325_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9735033.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            6406..7470
                     /locus_tag="VW325_001685"
     CDS             6406..7470
                     /locus_tag="VW325_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735034.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAAESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7541..8401)
                     /locus_tag="VW325_001686"
     CDS             complement(7541..8401)
                     /locus_tag="VW325_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9735035.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWRIIKLFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAHLTDNAVRFTICQGIFNPIFFSHEDLKSFLEEDGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGQVHGVYHLAQGVKTVLPREPFVPAAYMEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILG
                     QDFKTLAQEK"
     gene            complement(8523..9509)
                     /locus_tag="VW325_001687"
     CDS             complement(8523..9509)
                     /locus_tag="VW325_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9735036.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(9781..11256)
                     /locus_tag="VW325_001688"
     CDS             complement(9781..11256)
                     /locus_tag="VW325_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9735037.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(11514..12437)
                     /locus_tag="VW325_001689"
     CDS             complement(11514..12437)
                     /locus_tag="VW325_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9735038.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(12451..13380)
                     /locus_tag="VW325_001690"
     CDS             complement(12451..13380)
                     /locus_tag="VW325_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9735039.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(13699..13920)
                     /locus_tag="VW325_001691"
     CDS             complement(13699..13920)
                     /locus_tag="VW325_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735040.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(13874..14134)
                     /locus_tag="VW325_001692"
     CDS             complement(13874..14134)
                     /locus_tag="VW325_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735041.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            14214..14351
                     /locus_tag="VW325_001693"
                     /pseudo
     CDS             14214..14351
                     /locus_tag="VW325_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         4334 a         3088 c         2666 g         4365 t
ORIGIN      
        1 aaccatatac ggcaaggcga cgttgacgtg gtttgaagag attttcgaag agtataagtc
       61 tctacataat aaaacgcata ttaccaaggt tgtgtaactt gatactatgc gtttttcttt
      121 tttattcaat gtgggttgtt tgattagcaa aggcgataat agcttgcttc aactcatctc
      181 cactgagagt gcggaactct ccaatctttt gattgatgga ttctaaaggc atgacattaa
      241 ccttaccaac gaaaatctta tccataactt gattatcaac gagttcggtt gataccaaga
      301 gttcttcgta gagtttttca cctgggcgga ttccaacttc aacgattgga atttcacttt
      361 cagtgtggcc acttagaagc accatcttct tggccaagtc ataaatcttg actggtttgc
      421 ccatatcaag gataaagact tccccatctt tggcataagc accagcatgg ataaccagac
      481 ggctagcttc tggaatggtc ataaaataac gggtcatgcg gaagtctgtc accgttacag
      541 gcccaccttc agcaatctga cgttcaaaga ctggaatgac actaccacgg ctaccaagaa
      601 cattcccaaa acgaactgca cagtaggttg attggctacg ttggttaaag ccagtgacaa
      661 tcaactccgc cacgcgcttg gttgctccca taacatttgg tggattgact gccttatctg
      721 tcgaaatcat aaccatctta gacactttag cttcatcaac agccttagca acattgtaag
      781 ttccacggat attgtttttg aaggcttctt ttggattgcg ctccatcata ggaacatgct
      841 tgtgggctgc cgcatgataa acaatagcag gtttgtactg ctcaaagact tgcaacaaac
      901 gatcatagtc ttgaatgtcc gcaatcacag gtacataatc aatcccttgg aacttacgaa
      961 tcaattcatg ataaacaagg tagattgagt tttccccatg accgagcaag acaatgcgtt
     1021 caggattgaa gcgactaact tgacgacaga tttcagaacc gattgaacct ccagctcctg
     1081 tgactaagat ggtcttacct gtcagttctg cacccagacg cgattcgtca agacggattt
     1141 cctgacgacc caaaaggtcc gtaatatcaa ttttttggaa gccagtacct gcttggtgaa
     1201 ggccctgaac aacagtttca accttaggca tcttgtaaca tttgacaccc agcttattac
     1261 acatctgcaa gatacgctca tattctgacg gatccagcga cggaatcgca acgatgacac
     1321 gctcgatttg atggcgtttg gctaattcag gcagattgtc ataagagccc aaaacaggaa
     1381 taccaccaag tttttgaccc tttttcttag aatccttatc caaaataccg accagttcta
     1441 attcactggt tggatgttgg taactatcca taaaaagagc cccaccatca ccggcaccaa
     1501 tcaagaaggt ccgacggtgt tctccatcac cactaccttt tttgcgtctg gagtagatta
     1561 actgccaagt aatccgtggc aataaaatca agaaggtact caacaagata aagagaatga
     1621 tgaaacggat ggagaagagt ggcaagaagg cataacagat actatatgac aagacactgc
     1681 tagcagtcac accaaaaaag attttcatga aatccgtaat cttgctgtaa cgactaatgc
     1741 tcgcgttcaa cccccaaaaa ccaatcatca attgatagaa caggaaggcc aaactcgtat
     1801 agataatgta gtcaacaggt gctggattaa tcagcccata aaataaaata taagatacaa
     1861 tgatggaaac caccatactg aaaatatcaa atattcccca gaaaacctgc ttttgttgtt
     1921 tatttaaaat ttccaccaga tcaatcacat aatctgttag ttttttattc atagaaatct
     1981 actcctcctt aaaagagttt tactatattg ttattatagc atattttgat ttatttggct
     2041 tattttatcc aaataaatta ttgttccctt gtactccttc ttccatttaa atcctctaat
     2101 taaacctaca atcgtcccaa agccataagc aaagtgaatg gaaaataaaa gaaagggcat
     2161 cacaattaga aatccatttt tatgttttaa taaagtcagc aaagtgagta gtgacaaaag
     2221 tagaaaatag gcacctaata gtaaagttat gaatacgaat gtgatcggta acaatgctag
     2281 actaaacaca agactcaaaa caaataaaca aggaacatag tgaaataatg ataaacactt
     2341 aaactgaaca tgacttgtca agccaatcca caaaccattt gaatactttt gatgcagcat
     2401 tttcttgaat gttggtcgaa tatactgata agatagaata cttgggctat agcggatttt
     2461 ataaccatgt tctcgaattc tataatgaat atcattatct tcagttcggc caagttgctc
     2521 atttactaaa ccaaccttct ggaaaacctc tcgtttatac attccatgaa aaatagaaga
     2581 aacatatcta tcctcggaac tatttcgata attggcaatg ctactgccaa acatattttc
     2641 ctcaacaaga tgcaaggtct ctgcccattt tccttttcct tcgacaatcg tcggtctagg
     2701 ccccccacag acaaattcac cttgttgaat aatagccaca ttgttcatta caaaagtctc
     2761 agtaactttt gaatgagcat caatttttaa aataaggtcc cctacagaat gtttaactcc
     2821 caggttaaaa ccactagctt gatttttctt aggattgtta tacaatctaa ttgagttaaa
     2881 ctctgtatct tcctttataa attgctgaat gatagctgtg gtcccatctg tggacatagc
     2941 atttataaat agaatttcaa tattctcttt aggataagtt tgatttttta agtcttcaat
     3001 tagaccaggc agatattttt cttcattgta tgcactgatt acaatggata cgagaagact
     3061 ttcaacattt tgagtcacta ctactctcct gaatttttcc ttccaccatt ctatcataat
     3121 tttcaagact ttcccatttt tatcttgaaa gccagaaacc ttacttgaca ttatagtgaa
     3181 aaagccttaa aataagctgt tattaaaatc agctagaaga ggtattatat gaaaaagcaa
     3241 tcactctttt ttgttccagg tattatcctg attggtgttt ccttgcgaac tccttttact
     3301 gttttaccca ttattttggg aaatatttcg caaggtctgg aggtagaagt tagttcgctt
     3361 ggtgtcttga ccagcctgcc tctccttatg tttaccctct tttcaccatt ttctacccaa
     3421 ctggctcaga aaatcggctt ggagcatctc ttcacctaca gcctcttctt cttgaccatc
     3481 ggctcactta ttcgactaat caatctgccc ctactctatc taggaacctt gatggttggg
     3541 gcaagtgtcg cagtcatcaa tgtcctgctt cctagtctta tccaagccaa tcaaccaaag
     3601 aaaattggtt ttctgaccac cttatatgta acgtctatgg ggattgcaac ggctctggct
     3661 tcctatctag ctgtgcccat tacacaagcc agttcttgga aaggacttat ccttctcctc
     3721 acgttactct gtctagcaac ttttttggtc tggctcccaa atcaccgcta taatcataga
     3781 ctagctccac aaaccaaaca aaaaagtcaa ataaaggtca tgcgtaataa acaggtttgg
     3841 gcaattatta tcttttcagg ttttcaatcc ttgatctttt acaccgtcat gacctggtta
     3901 cctaccatgt ctatccatgc aggtctatcc agtcacgaag ctggcttgct gacttctatc
     3961 ttatctctga ttagcattcc tttttcaatg accatcccaa gcctgacaac cagtttatct
     4021 actcgcaacc gtcagctcat gctcactctg gtttcactag ctggtgtggt cggcatttcc
     4081 atgctctttt tcccaatcaa taatttcatt tactggcttg ccatccatct cctcatcgga
     4141 accgcaacca gtgccctctt cccttatctc atggtcaact tttcactcaa gacaagcgcc
     4201 cctgaaaaga cagcccaatt gtccggccta tctcaaacag gaggctatat cctagcagcc
     4261 tttgggccaa ccctctttgg ttacagtttt gacctgttcc actcttgggt accatctgta
     4321 gctgccctct tgctcatcga tatcctgatg actgtggccc tctttacagt ggacagagcg
     4381 gataagatcc tttaaacttc tagttttagc tagaagtttt ttatatataa aaattttaca
     4441 catttctctt gacagggctt tcttttagat gtacaatgta tgtgtagaaa aattatatat
     4501 aaaaatctta cacattagaa aaggaggttt cccatgtact ttccaacatc ctctgccttg
     4561 attgaatttc tcatcttggc tgtactggag cagggtgatt cttatggtta tgagattagc
     4621 caaaccatta agctgatcgc taatatcaaa gaatccacac tctatcccat tctcaaaaaa
     4681 ttggaaggca atagctttct gacaacctat tctagagagt tccaaggtcg catgcgcaaa
     4741 tactactcct tgacaaacgg tggtatagag cagctcttga ccctaaaaga tgaatgggca
     4801 ctctatacag acaccatcaa tggcatcata gaagggagta tccgccatga caagaactga
     4861 atacctgact cagctagaac tctatctcaa gaaactacct gaagctgacc gtatcgaagc
     4921 catggactat ttcagagagc tctttgacga tgctggagtc gaaggagaag aagaactcat
     4981 cgctagtttg ggaactccca aagaagcggc ccacgaagtt ctatccaatc ttctcgataa
     5041 aaaaatcaat gaagcacccg ctcaaaaaaa taaccgacaa attttacata tcgccttgtt
     5101 agccctcctt gcagcaccta tcggcattcc tctgggaatc gccatcctcg tgaccctgtt
     5161 cgcaattctt gtagccgctt tgactgtcat tctggctttc tttgcagttt ccatactggg
     5221 tatcatcggc ggattcctat ttttagttga aagtttcact gtcctcgccc aagccaaatc
     5281 agcctttatc ttgatttttg gttctggttt actggctatc ggtgcttctt cgctagtttt
     5341 acttggcatt tcctatgcaa ctcgcttctt cggtctactc attgttcgtc tggtacaatt
     5401 tgttcttaaa aaaggaaaga gaggtaatca gcatgcgtaa atggacaaaa ggatttctca
     5461 tctttggtgt ggtgactacc gttatcggct ttatcctgct ttttgtaggt atccaatctg
     5521 acgggattaa gagcctactt tccatgtcca aagaacctgt ctatgatagc cgtacggaaa
     5581 agctaacctt tggcaaggaa gtcgaaaacc tagaaattac tctccaccaa cacacgctca
     5641 ccatcacaga ctctttcgat gatcaaatcc acatttctta ccatccatct ctttctgctc
     5701 accatgatct tatcaccaat cagaacgata gaactctgag tctcactgat aagaaactgt
     5761 ctgaaactcc gtttctctct tctggaattg gtgggattct tcatatcgca agtagctact
     5821 ctagtcgttt tgaagaagtt attctccgac taccaaaagg gagaactcta aaagggatca
     5881 acatctcagc caatcgcgga caaaccacca tcataaatgc tagccttgaa aatgcgaccc
     5941 tcaatacaaa cagctatatc ctccgaattg aaggaagtcg tatcaaaaac agtaaactca
     6001 caacgcccaa tatcgttaat atctttgata cagttcttac agatagtcag ctagagtcaa
     6061 cagagaatca cttccacgct gaaaatatcc aagtccatgg caaggttgaa ctgactgcca
     6121 aagattatct cagaatcatc ctagaccaga aagaaagcca acgaattaac tgggacatct
     6181 caagcaacta tggttctatc ttccaattca caagagaaaa gcctgaatca agaggtacgg
     6241 aattaagcaa cccttacaaa actgaaaaaa ccgatgtcaa ggatcaactc attgcgagat
     6301 ctgatgataa tattgatcta atatccacac caagcagacg ttgacaaaaa cgcttacatc
     6361 tgctactatg gaagcatatt tatctaacca aaaggaggtt ctaccatgaa acaagaatgg
     6421 tttgaaagta atgattttgt aaaaacaaca agcaagaaca agcctgaaga gcaagctcaa
     6481 gaggttgcag acaaggctga agaaacgata gccgatctcg atacaccaat tgaaaaaaat
     6541 actcagttag aggaggaagt ctctcaagct gaagtcgaat tggaaagcca gcaagaagag
     6601 aaaattgaag ctcctgaaga cagtgaagcg agaacagaaa tagaagaaaa gaaggcatct
     6661 aattctactg aagaagagcc agacctttct aaagaaacag aaaaagtcac tatagctgca
     6721 gagagccaag aagctcttcc tcagcaaaaa gcaaccacga aagagccact tcttatcagt
     6781 aaatctttag aaagtcctta tatccccgac caagctccaa aatctaggga taaatggaaa
     6841 gagcaagtgc ttgatttttg gtcttggcta gtggaagcga tcaaatctcc tacaagtaag
     6901 ttggaaacaa gtatcacaca cagttacaca gcctttctct tgctcattct gttttctgca
     6961 tcttcctttt tctttagtat ctatcacatc aaacatgctt actatggaca tatagcaagc
     7021 attaacagtc gcttccctga gcagctagct cctttaactc ttttttctat catctctatc
     7081 ctagtagcga caacactctt cttcttttca ttcctcttgg gtagtttcgt tgtgagacga
     7141 tttatccacc aggaaaagga ctggacgcta gacaaggttc tccaacaata tagtcaactc
     7201 ttggcaattc caatcttcct cactgctatt gctagtttct ttgctttctt tgatagccta
     7261 cgatttacag ccctcttgtg tgtgattagc attggaatca ttctgcttgc cagtctccat
     7321 atcattacaa gaccaagtca agcaagtgaa accgactcct tctatcaatt attcttgtct
     7381 gtccttgtga acggagttat tatcctcctc ttctttgtag ctgaagtggc actgattgga
     7441 gattatcttc gtatcttggc ctttctttaa aattcaatcc tgtcatcctt ggcaggattt
     7501 ttctatttca aaaaagcaag tcgattgacc tgcttctgct ttacttttct tgtgctaatg
     7561 ttttaaaatc ttgtcctagg atgggttgta tttctatttg attggcgtct agttggtggt
     7621 aaaaagatgg gggtaatacc tctaagaatt ttgcatagtc caagcgagct atggattcat
     7681 agtcttctgg tttagagccg tctcctgtga tttgaaccaa gtcaatctcc cactggactt
     7741 ccttatccac caattgctcc atataggcag caggaacaaa tggttctctc ggtaaaacgg
     7801 tcttgacgcc ttgagccaaa tgataaacac cgtgcacttg cccttctcca tcctgataga
     7861 aggaaactct tgcaaagtag gcatctgtct cttgaaccgc acgcacacgc gcttcaaact
     7921 gagccaaacc atcttcctct aaaaagcttt tcaagtcctc atgactaaag aaaataggat
     7981 taaaaattcc ttggcaaatc gtaaaacgaa ctgcattatc tgtcagatgg gcttgaatac
     8041 ttgcttggaa ataagcttca aaatcaaaac catctagtcc ttcaatatca gtccctgtat
     8101 aagcaagcag agcatctaaa aataacttga taatccgcca gtctgtcttc gtgagtaggt
     8161 caggaagatc gacacggtaa gccttttgac catcagcata actgacctta aaaagaaatt
     8221 gtgattgccc cactatcgca cattcgatat actcgagacg attaagaggc tgacggagat
     8281 agactacatc atagctatgt gactccaaac cgtctagcaa ggccaagatc gacttggcat
     8341 tcaaaatctc ttgttctcct aaaatgctct gtttatttgg aataaaaaat gttttcgcca
     8401 taactggact cctttgaaat cgtttacata cagtatacct tatttttctg aaagatacaa
     8461 aaacaaactt tagatttttg agtacaaagc atagaaaaac agcccctgag gactgtttga
     8521 tcttatacag tagctgcctg atccaagctt tcaccgatag cggctaggcg ctcaatcact
     8581 tcagcttgtg tcaattcatt ttttgaaaca tagcggttac gtgggtgaac acggcactcg
     8641 tgtgagcatc cacgaaggta cttgtcttca ttttcttctg atgtcaagat acgacggtta
     8701 cagaagggat ttccacagtt aacataacgt tcacatggtg ttccatcaaa ccagtctttc
     8761 cctacgatgg ttgggttgac atggttgaca tcaacggcga tacgctcgtc aaatacgtac
     8821 atttttccat cccaaagctc accttgaact tctgggtctt taccgtaagt tgcgattcct
     8881 ccgtgcaatt ggccgacatc tttgtagcct tcacggacca tccagcctga gaatttctca
     8941 cagcgaacgc cacctgtaca gtaaaccacg acacgcttgt ccatgaattt ttccttgtta
     9001 tcacggaccc attgtggtaa ctcacggaag ttgcgaatat ctgggcgaat agctccacgg
     9061 aaatgtccta ggtcgtactc ataatcgtta cgtgtgtcaa ggacaacggt atctttatca
     9121 agaagcgctt ctttgaactc ttttggagac aagtaagcac ctgttgtttc aagtgggttg
     9181 atgtcattgt caaagtcgtt gtcttccaaa ccaaggtgga caatttcttt cttgtagcga
     9241 acaaacatct tcttgaaggc ttgttcattt tcttcgtcaa tcttgaacca gagttcttcc
     9301 attcctggaa ggctgtgaac gtagtccatg tatttttgag ttgtttcata gtcacctgaa
     9361 actgttccgt taattccctc gtcagcgact aggatacggc ctttaaggcc gattgattta
     9421 cagaaagcca agtggtctgc agcaaattgc tctgcatttt caattggagt ataaaggtag
     9481 taaagtaaga cacgaatatc ttttgccata agatttgttc tcttttctat tcttaaattt
     9541 tctaaatttt ttatttatct acactagtat acctgcttga gaaaacgaat gcaatttatt
     9601 tgactggaaa ttgtagaggg ttctcgaaat ttcctgaatg gttaaaataa ggacaagaga
     9661 aaacatggat atctatatcc ttgtgccaaa aaaaccactg ccctccccag accaacctga
     9721 ggaaagcagt gattcttatt ttaggagtta ggaatgaata cacgaaatca atttagctga
     9781 ttattttttg tttttcaaga attcatcgta ttgtttttgc atttcgttca atactttttc
     9841 gtaggcacct tcagatttca atttttccat caattctgga atcgctttat ctgggtctac
     9901 agtaccagtg ttgatagctg tatcaaattg ttgcattgtg ttagcaatag ctgagatttc
     9961 agatttcaca ttgtcagtat tgaagataaa tccaagcgct ggagattctt tagcttctgc
    10021 caattctttc ttagaatttt cgatttgttg gtctgtaacg ttttcgttga tgtaaaggat
    10081 ccagttgtta ccagtgttcc atccacccat gtgagtgttt cctttgtagc catcaagaac
    10141 gcgaacacgg ttttctttac cttcaatttt ttcccagttc ttgccttctg gaccgtaaac
    10201 aagaccgttc aagagttctg gattcgtatt caagaggttc aagatttcca ttgatttttc
    10261 tttgttctta gagttgtttg agatgacaaa gttagcaact tgtgttgttt ggtttttctt
    10321 gatgaagtta gtaattggtt tgatttggat atctttgttg gcaacacgtg aaagcaagct
    10381 gttaccgtag tcagctggtc ctactgtttc ttcacgaacg aaccaagtat cttgttgaag
    10441 gtcaaaggaa gtatcgcttg ttgcgacgtc ttttggaatg tagccagctt catagaattt
    10501 gtgaagagtc ttcaagtgtt ctttgaaacg aggcacttcg taacggttta caactttagt
    10561 agtatcgcct tcaaggtcga taacgaatgg aagaccgttt gctactgggt agtcaaagtt
    10621 atcagatggg atgaaaactt taccaatagc aaatggtact acgtctggag ctttttcttt
    10681 gatttgtttc aagactggct caagagtttc gtaagaagta acacctgaaa tatcgatacc
    10741 atatttagca aggagagttc cgttgaaggc aaagttttga gatgatgcaa cgttggctgc
    10801 aactggaaca gcgtaaatct taccatttac agtattaccc ttgatgtaag ctgggtcaag
    10861 tgctttgtaa aggtctttac cttctttttt gtacaattct gtcaagtcag cgtaagcacc
    10921 tttttgagca tttacaatat agttatctgc aaaggcaata tcatagtttt caccagatga
    10981 tgtgataact gacattttct taccatagtc accccagcca aggtattgga tatccaattt
    11041 ggcaccaact ttttcttcaa tgattttgtt ggcatttgct aacaattcat ccaagttgtc
    11101 tggtttgtca ccgatttggt acattttgat aacaggtttg tcacctgaat cagcagcttt
    11161 tttgctgtta cctgtcaaat ttccacaagc agcaagacct gcagccaaag cgactacact
    11221 agcagatgca aaagcatatt ttttccagtt tttcatgata aaaactcctt tttttatttt
    11281 taaacttata aacaatgtaa tgatcttata ctcaatgaaa atcaaagagc aaactagaaa
    11341 actagccgca ggctgctcaa agcactgctt tgaggttgta gataagactg acgaagtcag
    11401 ttacatatat ctacggcaag gcgacgttga cgcggtttga atttgatttt cgaagagtat
    11461 taacttcaca caagggaagt tgggaactga gaaatgttct ttttcaataa gcactattct
    11521 ttcacaccac cgatagtcaa accttttaca aagtagcgtt ggaagaatgg atataaaatc
    11581 gcgattggaa gggttgcaac cacaaccatg gccatacgac ctgtttcttt cggtagagca
    11641 actcccagtt gaccagtcaa gccgaccgct ttggcaatgt agtccatatt ttgttggatt
    11701 tgcatgagca aatattgcaa tggatacaag ttatcactct tgatgtaaag aagggcgttg
    11761 aaccagtcat tccagaaacc aagagctgtc aaaagcgtga tggttgcgat acctggtagt
    11821 gacaatggca aacagatttg gaagaaaatc cgggcctcac tggcaccatc gatacgagcc
    11881 gattcgagaa tggcttctgg aatggtcttc ttgaagaagg aacgcatcaa gatgatattg
    11941 aatggtgaga gaagcattgg aacaatcaag gcccaaacag tgtcaccaag ctgaagtaca
    12001 cgggtcacca tgatataacc tggtaccaaa ccagcgttga acaacatact gagaaggacg
    12061 aagatggtaa aaaatctgcg atacttaaag gttgtccgtg aaatagcgta ggcataggtt
    12121 gttgtgataa agacatttgt caatgtccca actacggtta caaagacaga gatgaagagg
    12181 gcttgtagga ttttatcctt aaactgtgcc aaaaactcaa aaccgtctaa gccaaattgg
    12241 gatgggaaga agctatagcc gtattggagg aggcttttct cgtctgtcac tgaaataatg
    12301 ataacgaata caaaaggtag gatacaagag agggcaatca aacccgaaat gatactgaag
    12361 aagatatctg ctttcttact gaaggagtga atgccgacat tatcaatttt ttctttttta
    12421 attttctttt ttgccatatt ctcctccttt ctagaacaaa gctgagtttg gatcgactcg
    12481 tcttgcaagc aagtttgata ggataaccag aatcaaacca acaacggatt ggtaaagacc
    12541 ggctgctgca gccataccga tatctgctgt ctgagtcaaa ccattaaaaa catatacgtc
    12601 caaaacgttg gttacattgt aaagctgacc agcattgtgt gggatttgat agaagagacc
    12661 gaagtctgcg cggaagatat ttccgactgc aaggatggtc aatacagtta caagcggagt
    12721 caactgagga atggttacgt tgcgaatacg ttgccacttg ctagctccgt ccactgtcgc
    12781 tgcttcgtag taggttggat caattcccat gatcgtcgca tagtacatga cactgctata
    12841 tccaaaacct ttccaaatac ctaggaaaag taggagatag ggccagatgc ccaggtcagc
    12901 gtagaaattg acttctttga gaccaagact ttccaataga tgattgaaca cccctttatc
    12961 aatatttagg aaggcatctg taaagaaact gatgataacc caagacaaga agtaagggaa
    13021 caacatagaa gtttggaaaa tcttcaccat tctcttagaa cggagctcgc tgaggataat
    13081 ggcaatccct acagatacaa ctaaacctag aaagataaag ccaagattgt agaggacagt
    13141 atttcgtgtg ataataaagg cgtctcttga actaaataag aatctgaaat tatcgagtcc
    13201 gacccattta ctatttatga tactatctat gaaaccatta ctggtcatgt ggtagtcttt
    13261 gaaggcaacc acgttcccaa atactggaat gtaaaagaat agaatcaacc agagtgcccc
    13321 tggcaaaacc atcaagagaa agatccagtt gtctctcaat gtttttgaaa actttttcat
    13381 aatttcctcc ctttttattt tgatatccat ctaaaaattc ttttttagac ttttgataac
    13441 gattacatta ttagtatact cctatttgca ggttaggtta aactcctaat tatagaaaaa
    13501 actccacaaa ttatgtagca gatttaaaac tttatcacca ctatcaaaca aatgtcctaa
    13561 atcaattgtt tattttatct ctattagccc agtgatggcg tcactctgtt ataagcatcc
    13621 aacaacgggg tatactgaaa aatctccaga ctagggaact cagagatagt tcctaatctg
    13681 gagattttta atatgttatt aggcgtttgc tttcaactta gcaataacct ctttaagatt
    13741 atcaatcacc tctgcttcag tatgctcaga gcctttttca tctgccaaga acaaaactgc
    13801 tttttgaagt tctttttgag agttttcaag gacatcctta tctactgttt caaggtttga
    13861 gtctttaaga agtttactta attccttggc taatttcttg agtttgattt gcagactcat
    13921 cttctcctgc tgtttctttg cccgctgttt gtcctccatc cttagttgct gactggcttt
    13981 ccttaatgga ctctagggaa gcaatggcat ctttgactgt ttgcaagata tcacgtaaac
    14041 cttgctctgt caaactatca tctgcaaaag ctttcttagc ctctgccaaa accagacgtg
    14101 ctgaatctgt ggtaggattc gatacacctg tcaatgatct caaaagattt tctaaggttt
    14161 gagtctgctt actaatacta gactaaaatc aaaaagtatt atataacagt gatatgaaat
    14221 caactaaaga agaaatccaa accatcaaaa cacttttaaa agactctcgt acagctaaat
    14281 atcataaacg ccttcaaatc gttctatttt gtctgatggg caaatcttat aaagagatta
    14341 tagaactttt atagtagttt gaaataagat gtgaacatct ctatcaggaa agtcaaatta
    14401 atttatagaa atattttagc agccaaggtg tactgttata gattcaatac act
//
LOCUS       DAVKLX010000049        14353 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKLX010000049 DAVKLX010000000
VERSION     DAVKLX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.049"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            8..1009
                     /locus_tag="VW325_001694"
     CDS             8..1009
                     /locus_tag="VW325_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9735042.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1617..1694
                     /locus_tag="VW325_001695"
                     /pseudo
     CDS             1617..1694
                     /locus_tag="VW325_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2150..3511
                     /gene="trpE"
                     /locus_tag="VW325_001696"
     CDS             2150..3511
                     /gene="trpE"
                     /locus_tag="VW325_001696"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9735043.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3508..4074
                     /locus_tag="VW325_001697"
     CDS             3508..4074
                     /locus_tag="VW325_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9735044.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEEGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4085..5089
                     /gene="trpD"
                     /locus_tag="VW325_001698"
     CDS             4085..5089
                     /gene="trpD"
                     /locus_tag="VW325_001698"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9735045.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5086..5853
                     /gene="trpC"
                     /locus_tag="VW325_001699"
     CDS             5086..5853
                     /gene="trpC"
                     /locus_tag="VW325_001699"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9735046.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5840..6439
                     /locus_tag="VW325_001700"
     CDS             5840..6439
                     /locus_tag="VW325_001700"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9735047.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGEGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLPQSFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            6417..7640
                     /gene="trpB"
                     /locus_tag="VW325_001701"
     CDS             6417..7640
                     /gene="trpB"
                     /locus_tag="VW325_001701"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9735048.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7633..8409
                     /locus_tag="VW325_001702"
     CDS             7633..8409
                     /locus_tag="VW325_001702"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9735049.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLANTDIALIPLVSLTTGI
                     ERQKELIAGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(8381..8482)
                     /locus_tag="VW325_001703"
                     /pseudo
     CDS             complement(8381..8482)
                     /locus_tag="VW325_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            8711..9442
                     /locus_tag="VW325_001704"
     CDS             8711..9442
                     /locus_tag="VW325_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735050.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            9457..10608
                     /locus_tag="VW325_001705"
     CDS             9457..10608
                     /locus_tag="VW325_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9735051.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRASNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSICLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(10660..11319)
                     /locus_tag="VW325_001706"
     CDS             complement(10660..11319)
                     /locus_tag="VW325_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9735052.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFILQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            11389..11835
                     /locus_tag="VW325_001707"
     CDS             11389..11835
                     /locus_tag="VW325_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9735053.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
     gene            11980..12252
                     /locus_tag="VW325_001708"
                     /pseudo
     CDS             11980..12252
                     /locus_tag="VW325_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12363..12566)
                     /locus_tag="VW325_001709"
     CDS             complement(12363..12566)
                     /locus_tag="VW325_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9735054.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(12597..13205)
                     /locus_tag="VW325_001710"
     CDS             complement(12597..13205)
                     /locus_tag="VW325_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9735055.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            complement(13244..13414)
                     /locus_tag="VW325_001711"
     CDS             complement(13244..13414)
                     /locus_tag="VW325_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9735056.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            complement(13426..13992)
                     /gene="amaP"
                     /locus_tag="VW325_001712"
     CDS             complement(13426..13992)
                     /gene="amaP"
                     /locus_tag="VW325_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9735057.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(14076..14240)
                     /locus_tag="VW325_001713"
     CDS             complement(14076..14240)
                     /locus_tag="VW325_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9735058.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAEMALLPSIVGAAIVI
                     TVVSFFTGRK"
BASE COUNT         4516 a         2566 c         3104 g         4167 t
ORIGIN      
        1 gactgaaatg aaaaatataa atgggaaaaa ggtaactatt tatgatattg cacgtttatc
       61 aggtttttct ccaaaaacag tttcacgagt tattaatggt ggagtaaatg ttaaagaaga
      121 aacttatcaa gcgattcaaa aagttataga ggaattgtcg tatattccaa atgcgtatgc
      181 aaaaaattta actaaaaaag aagctataaa tatattgatt tctgtaaaaa aaatcgactc
      241 ttttccttta atttggtttc atacgctgtt agataaagtg ttacggacgt gtaaagaatt
      301 tggtgttaat gctatagttg agtactttgg agaggaggat acaattagta attcaattat
      361 atcaagcaca ggtagcctag tagatggtgt tattgttttt tatgaaagtg tagatgatat
      421 cagaattcag tatttaaaga agaaccatat gccttttctt gttttcggtg aatctcaaac
      481 gtctggagta gtctatgtat ctaataataa ttttcaagct acttatgata tgatgaaagc
      541 tgtaactgaa gaaaaattta aaaatatgtg gttgcttatg ggaggagaat ctcatgttaa
      601 taaggatcgt gaaagaggtg tgcgttcttt tctgaatgat aaaaattact ttatggattt
      661 aaaagtcatt tatggcttat ctacaattga ttcagtttat tcttatgcaa tgcagcactt
      721 gactacccaa aattatccgg acattatttt tgtttcagga gatgaaaagg ttcagggctt
      781 gattcgtgcg tgctatgaga aaggaattct tattccagat gatatttcta ttattggatt
      841 tgataatatt cctatttctc agtattatac tccagctttg tctacaattg ctcccaatta
      901 tgttaaattg gctaaagaaa tgatagaagg tgttttagca attattaaag gggaaagtgt
      961 cacatctgtt gaagtttctc ctaaatttgt taggagacag agtttctagt attgtttaat
     1021 tcttgaaaat cctagtggaa aagatggagt gttctaaaat aagttctgtt tagcctccat
     1081 tcagtttcta ttgagttgga tccaatttct aacaagtggg acatgttcat gttcgatcta
     1141 aggaagacat ggtcatattc gaatgatatt ttcaaaaaaa tcgtaaaaaa gtcttgacaa
     1201 agaaaaaaca aagtattaaa ataagttcaa caaatcagaa aagtaactat ttttgatctt
     1261 cagggagcct gtggtgattg tgaataggtg gttggaagta gtgaaagtgg gctgattttg
     1321 aaaatgaatt tgaaacaatg aaaattcggt gcgcacacct tacagtgcag cttgttgtta
     1381 aacaaggcag agatgtaaag ggatagtccc tttataattg aggtggcacc gcgttaccaa
     1441 cgccctcaca cggaagtata ttctgtgtgt gggctttttt ctatccgtcg tttggtttat
     1501 cttttattag ggccttaagt cgctttgatg aacttgagtt ctatctacag ctccatgcct
     1561 actactaaaa gcaaacaaaa aggctggact aatacgaaaa aagctagtaa tactcaatga
     1621 aaatcaaaga gcaaactagg aaactagccg caggctgctc aaaacactgt tttgaggttg
     1681 tggatagaac tgacgaagtc agtaaccata cctacggcaa ggcgacgttg acgcggtttg
     1741 aagagatttt cgaagagtat aaaggtgtac cgagatgaac atctcttcat caatgtctct
     1801 ggtcgtggag gcaaggacct agctgcgatt gcagattact agaagctaaa aaataataga
     1861 tggaaaaatt acagttcttt ctctcacaga gagcttgtgg ttgctgaaaa caagcagaga
     1921 aagctgtaaa gttgggttca tctgaaacga gagaagaaaa catcattctc aagggagtgc
     1981 cctttatcgc acagcctgtt acaggagata tctgagacag gaatgagaga taggagaaat
     2041 cctataattg aggtggcacc gcgaatttcg tcctcacgca agttattttg cgtggggatt
     2101 tttcatacag tcaccacgga ctagaagtag aactgaaagg gaaattacaa tggaacgaat
     2161 cattcatgga gatgtcttat caccaatctt ggcttatatg cgcttaaagg ggcaacataa
     2221 ggttatttta gagagtattc cgagagacaa ggaaacagct cgtttttcta tcctagccta
     2281 taatccagtt tttgagatta agtttgaaaa tggggttctc tatcaaaatg gtcaagtgat
     2341 tgatcgggat cccttggatt tcctttatga agtgattcat aagagtcagc accactcaga
     2401 gctacctttt ggtggtggag ccattggttt tgtgggttac gatatgattt cgctttatga
     2461 agaaattggt caaatccctg aggatacaat tgggacgcca gacatgcatt tctttgtcta
     2521 tgagagctat atggtctttg accacaagaa ggagaagata catgtcatag aggatgcact
     2581 ttatagtgag cgcagtcaag aggccttgga aaaatccttg aaccaagtgc ttgaggaatt
     2641 acgcattcct gctccaaatg aatttgaaga tttggatcta tctccgttag acttcaaacc
     2701 gcatatcgct ccacataagt ttgagggaat ggtagaaaca gctcgtgact tgattcgtaa
     2761 cggagatatg ttccaatgtg tactcagcca gcgtttttca gcagaagtta ctggaaatcc
     2821 atttgacttc tacagaaatc tccgcgtgac taatccttct aattaccttt atttctatga
     2881 ttttggggat tatcaaatca tcggagctag tccagaaagt ttagtttctg tcaaaaatgg
     2941 catcgtgaca accaatccga ttgcagggac gcgaccaaga ggggctacgg atgaagaaga
     3001 caagaccttg gcgactgacc tcctctctga tgagaaggaa acagcagaac atcgaatgtt
     3061 ggtagacttg gggcgtaacg atattggccg catctctgaa acggccagtg tccaagttac
     3121 taagtatatg aaggtggagc tcttccgcta cgtcatgcat ttgaccagcg tggtcaaggg
     3181 gcgtttgctt ccagaactca cggccatgga tgccttgaaa gctacacttc ctgctggaac
     3241 cgtttctgga gcaccaaaga ttcgggcgat gagacgcatc tatgaactgg aaacggaaaa
     3301 acggggcgta tatgcaggag caatcggcta cttgtctgcg acgggtgata tggatttggc
     3361 cattgccatc cgaaccatga ttctcaaaaa tcaaagagcc tatgtgcagg ctggggcagg
     3421 gattgtctac gactctatcg cccaaaacga ataccaagaa accattaaca aagcaaaatc
     3481 tatgactaga attggagaac taagaccatg attttattga ttgacaacta tgattctttt
     3541 acctataact tggcccaata cattgggaat tttgcagaag ttcaggttct gagaaatgat
     3601 gattccaagc tgtatgaaga agctgaaaaa gcagatggtc tggtcttttc tcctggtcct
     3661 ggttggccag ttgatgctgg aaagatggaa gacatgattc gtgattttgc aggcaagaag
     3721 ccgattcttg ggatttgttt gggccaccaa gccattgcag aagtctttgg tggtaagcta
     3781 ggtttggctc caaaagtcat gcatgggaaa cagagcaata tcaactttga agcgccatct
     3841 gttttgtatc aaggtattga ggaaggacgt gcggtcatgc gttatcacag tattttgatt
     3901 gaggaaatgc cagaagactt tgaagtgaca gctcgttcga ctgatgacca agccatcatg
     3961 gggattcaac ataaaaacct accgatttat ggcttccagt accatccaga gagcattgga
     4021 acgccagacg gcttgtcttc tattcggaat tttatcgaga aggttgtaaa gtgaggaaac
     4081 taggatgaaa gagattattg aaaaactagc aaaatttgaa aatttatcag gtgtggaaat
     4141 gacggatgtc attgagcgta tcgtaactgg gcgtgtaact gaagcgcaga ttgcttctct
     4201 cctcttagct cttaagatga agggggaaac acctgaagaa cgcacagcca ttgcccaagt
     4261 catgagagga catgcccagc atattccaac tgagattcat gatgccatgg acaactgtgg
     4321 tacaggtggg gacaagtctt tcagttttaa tatttccaca actgcagcct ttgtcttggc
     4381 tggtggcggt attcacatgg ccaagcatgg taaccgctcg atttcttcta aatctggttc
     4441 cgcagatgtc ctcgaagcct tgggaatcaa tcttgacctc aaaccagctg aactaggtaa
     4501 ggtctttgat aaaactggaa tcgtctttct cttcgctaaa aatatgcacc cagctatgaa
     4561 atacatcatg ccagctcgtt tggaattggg aattccaacg attatgaact tgactggtcc
     4621 actgattcat ccaatggctt tagaaacaca gcttcttgga attagtcgtc cagaactcct
     4681 agaaagtaca gctcaggttt tgaaaaatat gggtcgcaaa cgtgccatcg tggttgctgg
     4741 accagaaggg ttggatgaag ctggcttgaa cggaacaacc aagattgcac ttcttgaaaa
     4801 tggcgaaatc agcttgtcaa gctttactcc agaggatttg ggaatggaag gctatgctat
     4861 ggaagatatt cgtggtggga atgctcagga aaatgcagaa attttgctta gcgttctgaa
     4921 aaacgaagca agtccattct tggaaacgac agtcttgaat gctggtcttg gtttctatgc
     4981 taatggtaag attgatagca tcaaggaagg agttgccttg gctcgtcaag tgattgctag
     5041 aggcaaggcc cttgaaaaac tcagactgtt acaggagtac caaaaatgag tcaggaattt
     5101 ttagcacgaa tcttagagca gaaggcgcgt gaggtggagc agatgaagct ggagcaaatc
     5161 cagcctctgc gccagaccta tcgcttggca gaatttttga agaatcatca ggaccgcttg
     5221 caggtaatcg ctgaggtcaa gaaagctagc cctagtttgg gagatatcaa tctcgatgtg
     5281 gatattgtgc aacaggccca gacttatgaa gaaaacggag cagtgatgat ttcggtgttg
     5341 acagatgagg ttttctttaa agggcatttg gattatctac gggaaatttc cagtcaggta
     5401 gagattccga cgctcaacaa agactttatc atagatgaaa agcaaatcat ccgcgctcgc
     5461 aatgcaggtg cgacagttat cttgctcatt gtggcagcct tgtccgaaga acgcctcaag
     5521 gaactgtatg actacgcgac agagcttggt ctggaagtct tagtggagac tcacaatcta
     5581 gctgaactag aggtagccca cagacttggt gctgagatta tcggggtcaa caaccgcaac
     5641 ttgactacct ttgaggtcga cttgcagacc agtgtagatt tagcccctta ctttgaggaa
     5701 ggtcgctatt acatttctga atctgccatt ttcacagggc aggatgcgga acgactagcc
     5761 ccatacttta acggaatttt ggtagggaca gctcttatgc aggcagaaaa tgtggcccag
     5821 agaatcaagg agttgcagat tgacaaaggt taaaatttgt ggactatcga ccaaagaagc
     5881 ggtggaaaca gccgtttcag caggagccga ctatatcggt tttgtctttg cacctagtaa
     5941 aagacaggtg actttagaag aggcagctga gttggcaaag cttattcctg cagatgtgaa
     6001 aaaggttgga gtatttgttt caccaagtcg ggtagaactg ctggaagcga ttgacaaagt
     6061 tggcttggac ttggttcaag ttcacggtca ggtggcagat gatttatttg agaatttgcc
     6121 ttgtgctagc attcaggctg tgcaggtaga tggagagggt catgtgccca attctcaggc
     6181 agactatcta ctctttgatg cccctgtggc tgggagtggc cagccctttg attggggtca
     6241 actggatacg actggattac ctcagtcttt ctttatcgca ggtgggctta atgaagacaa
     6301 tgtagtaaaa gcaattcaac actttactcc ctatgcagta gatgtatcaa gcggagtgga
     6361 gacagatgga caaaaagatc atgaaaaaat tagaagattt atagagaggg taaagaatgg
     6421 catatcaaga accaaataaa gatggatttt acggaaaatt cggcggacgt tttgtcccag
     6481 aaacattgat gacagcagtt ttggagttgg agaaggccta ccgtgaaagt caggcagacc
     6541 caagtttcca agaggaatta aaccaacttt tgcgccagta tgtgggacgt gaaactcccc
     6601 tttactacgc aaaaaacttg acccagcata tcggcggagc caagatttat ctcaaacggg
     6661 aagaccttaa ccatacagga gcccacaaga ttaacaatgc cttaggacaa gtttggcttg
     6721 ccaaacgcat gggtaaaaag aaaattatcg cagaaactgg tgctggtcag cacggtgtgg
     6781 caactgcaac tgctgcggcc ctctttaaca tggaatgtac catctacatg ggtgaggaag
     6841 atgtcaaacg ccaagccctc aatgtcttcc gtatggagct tttgggagct aaggtcgagg
     6901 ccgtgacaga tggttcgcgc gtgctcaagg atgcggtcaa tgcagctctt cgttcatggg
     6961 tggctaatat agatgatacc cactatatcc ttggttctgc cttggggcct catccatttc
     7021 cagaaattgt tcgtgacttc caaagtgtca ttggtcgaga ggctaaacaa cagtaccgtg
     7081 acttgacagg acaaaatctg ccagatgccc tagtagcctg tgttggtggt gggtcgaatg
     7141 ccatcggtct cttccatccc tttgtagaag atgagtcagt agccatgtat ggagctgaag
     7201 cggctggact tggtgtggat acggagcacc acgcagctac cttgaccaag ggtcgtccag
     7261 gtgtccttca cggttccctc atggatgtgc tccaagatgc ccatggtcaa attcttgaag
     7321 ccttctctat ctcagcaggt ttggactatc ctggtatcgg tccagagcat tctcactacc
     7381 acgatattaa acgtgccagc tatgtccctg tgacagacga agaagccttg gaaggattcc
     7441 aactcttgtc tcgtgtggaa gggattatcc cagccttgga atctagccat gctatcgcct
     7501 ttgcggtgaa attggccaaa gaacttggac cagaaaagtc tatgattgtc tgcctatcag
     7561 gtcgtgggga caaggatgtg gttcaagtca aggaccgctt ggaagcagat gcagcaaaga
     7621 agggagaagc tcatgcctaa gacactaaca gaaaaattaa atgctattaa agcagctgga
     7681 aaaggaattt ttgttcccta tatcatggct ggtgaccacg agaaaggttt ggacggtctt
     7741 gctgaaacaa ttcacttttt agaagatttg ggtgtctctg caattgaagt gggtattccc
     7801 ttttcagacc ctgttgcaga tggacctgtt atcgaagaag ctggcttgcg cagtttagcc
     7861 cacgggacct ctacccaggc tttagttgaa accttgaaaa ccattgaaac agagattcca
     7921 ctggtcatca tgacctactt caaccccctc tttcagtacg gtgtggagaa ctttgtcaaa
     7981 gatctggctg atacagctgt gaagggcttg attatcccag acctgcctca tgaacatgcc
     8041 aactttgtag aaccattttt ggcaaataca gatatcgcct tgattccttt agtaagcttg
     8101 accacaggaa ttgagcgcca gaaagagttg attgcagggg cggagggatt cgtctatgcc
     8161 gttgccatca atggggtgac agggaaatca ggcaattacc gtgcagattt ggacaagcac
     8221 ttggcgcaac ttcatcaagt ggccgacatc ccagtcttga caggttttgg tgtatctagt
     8281 caagccgatc tggaacgctt caatgcggtg tcagatggtg ttatcgtcgg ttcaaaaata
     8341 gtaaaagctc tccaccaagg agagccgatt caggatttta tcagacaagc agtagcttac
     8401 caaaaataat caaacaagta gcaagtaaga ggaaaggcac gaaaggaagt ctttcctttt
     8461 tcttttgcag gagaaaggtc agttttatag tgaattgaat ctggaatatt acactacggt
     8521 tcctaaaata ttttcagaaa gcgatttgaa tttcccaata aatttgttca gtttttaatt
     8581 gattttacta tagaagatat aattttctaa attactctga cacagaactt taagaaaaag
     8641 ataattattg tagtaatttc tttaagaaaa tgatataatt cattaaaaag tatttggagg
     8701 aggaagaatt atgttgaatc aggatctctt tgattcgctt gaggcacaaa aaattgtaga
     8761 tactttgatg aaagggcaaa aagattatgt agatgaacgt ctagaaaaaa gagagacaat
     8821 gatagtgtcg aatggttatg catggacgcg acctaatcat attgatactg catttgcatc
     8881 agcagatttg tttgagtata aattacaatt agcaggacag acttggggat atttagaatt
     8941 tgaaacaaat acagaaaaat atgggaaagt attgttaatt ataaagggta agaagcgact
     9001 tacgaaccaa tttcctttgg tacaaaaaaa taagagtggc tacttatttg aatacgctca
     9061 gatgaataca ctttatctta atcaacattc ttcctacaaa aatgatgaag atagtcattc
     9121 ctttccaatt cagatggagt tagtttctga tgaaatgatt caagaaattg aacaagctac
     9181 taaaaattcg aatatcgaaa aatttatgat tttaacttat gaggcggact cagaaaacaa
     9241 tattatatct gtagatgttg ttatgcctga tgcacgaact ggtcagctac acttgattca
     9301 agatttgtct gagtatattc aatcaagttc gtaccatttc gaagaagcta aatatcaaga
     9361 tattcctaat ttttcagaat tatcggaaac agaagatttt gaaattattc caagaataga
     9421 aaaacaagaa ggtcaaaagt agtaaggagt atataaatgt ttaatggtcg ggtattgaaa
     9481 gaattacggc tgttaaatgg tttaagtaga gcagaattag ctcagagaat taatttaacg
     9541 gaacaagcca tttggcagtt tgagtccaac gaaacgaaac ctaaattatc aaccaaaatg
     9601 catttggcca accaatttca tgttgattta acttattttg aacaggaaga agagagcatt
     9661 cgatttgatt cttctgtaat tgcctttaga aatgcagacc tagcaacacg gaaaacaata
     9721 gatattcaaa ctatgtattt acataaggta gatagtttga ttgattattt tgaaagtttt
     9781 gtaattatac ctaatattat aattcatgac ctaagtaatg tagtgagtga atcttatcat
     9841 aagggagaat ccattgagga attggctctt tatgccaggg aaaaattagg tatttcaaaa
     9901 gataatcatg atttgcttta taaattagaa cgttcaggta tctatatcgt ggaacgatta
     9961 attaatggcc aagctgatgc ttatagcgca tggtcaaaat tgggaagacc ttatattgtg
    10021 ttgggaacga ataaatcatc tgtacgtcga aattttgact tagctcatga gctaggacat
    10081 attcttttac ataaatataa ggatatgaat gaagatggcg atcgtttgga gcaagaagca
    10141 aattattttg catcatgttt tttattgcca aaagaagagt ttttagtcaa atttgaagag
    10201 agggttggca agcgtgccag caatcctgat agttatattt tattgaagtc ggatttgaat
    10261 gtttcgatac aggctttaga gtatcgagct tttaagttag gattattgac tccaaagcaa
    10321 cattcttact tttatcgtca aattgcgcaa aaaggttaca aaatgattga acctttggat
    10381 gatcaaattt ttgttaaaaa accaagcaaa gtaaagagta ttctggacgt cgttttgagt
    10441 aatcatctag tcagtctagc gactataatg tctaaacaaa gtatttgttt acagtttata
    10501 agcgaaatat tttcagtcga aatgaaattt tttgatcagt atcaagaaga tagaagaaca
    10561 gatcgatttg ataacatcat ccctttgtac aaaagaaata atttataatt taactgtgaa
    10621 ataatttcat gaaggacata tggcagaaat tggattttat cagacaagca gtagcttacc
    10681 aaaaataatc aaacaagtag caagtaagag gaaaggcacg aaaggaagtc tttccttttt
    10741 cttttgcagg ataaaggcca ggatacccgt cgcagaagca aactgaatca agatcagtaa
    10801 ctccgttacg ctaaagacga gagcacaaga agctaaaaag aggaaatctc ctgcgcccat
    10861 gcggatatcg ataaaatgag ccaaaattcc aagagcaagg aaggagacca tgaccagatt
    10921 ccagccagag gaagctatta ggattaggtg gaaagtcatc cagaccagta agggatattc
    10981 ctgatggtga aagtcgtaga tacccaaggt caaaccagcg gtgattagga cgacttgccc
    11041 caaggaaagc aattcccaag agtaaagcag aaagaggagt cctaagacta attcaaagag
    11101 ggcataccag acaggatagc gaactttgca gtagcgacag cgaaagcgat tgaagacctg
    11161 tgagagaatc ggaatcaaat ctaagggacg caagcgagtc tgacaggaat cgcagtgact
    11221 ggctgaactg ataatggatt gctctggaaa acggtcaatg accaaaccaa gaaaggaagc
    11281 gagaatgctc ccgacgagaa aaaaataaaa atcaatcata cttatctatt cgtaaaaaat
    11341 gggagaaata gtataatgga gaaacataga taagatggtg aggaaaagat gacaattcgt
    11401 tttgaagaaa aggtgagtat agaaaacgct caatttgtat gccagtggtc caactcccta
    11461 ggcaaatcct ttcaagaaca atggatggga ccaaggattc cttttctact gacccttcaa
    11521 gccttggaag gagtcttttc aatctttgat gaacaagagt ttgtgggact tatccagaaa
    11581 atcaggctag aagacagcaa tcttcatatc gggagatttt ttatcaaccc ccagaaacag
    11641 gagcaagact taggtagcca ggctttaagg aaatttgtta gtttggcctt tgaaaatgaa
    11701 gatatagata gtatttctct aaatgtcttc gaggcaaatc aaagagctca gaatctttac
    11761 caaaaagaag gatttgaaat cgttcaaatg gttgaagcac ctgtacgaaa atatagtaga
    11821 ttgaaactag aatagtacac ctctacttct aaaacattgt tagaaatcga tttgactgtc
    11881 ccgatcgatt cgtcctgtta ttatttcatt ttactataca ttatgaaaaa ggggagatag
    11941 aaagcaaaca gccttgaaat caatgttgac tgcgattatg aacctgaatc agcccctcaa
    12001 aagggactgt atatgaatga acgttaccag tgtttaaaaa ctaaagaata tcaggcactt
    12061 ttatcttcca agggtagaca aattttcgct aaacgtaaga ttgatatgaa atctgtcttt
    12121 gggcagataa aggtttgttt gggttataag agatgtcatc tgagaggtaa gcgtcaagtg
    12181 agaattgaca tgggattcat actcatggcc aacaacctgc tgaaatataa taagagaaag
    12241 aggcaaaatt aaaaagctag agttccgcaa ttgggaatct ctagcttttt gttttctgag
    12301 aatcatcttg actcagactc ttttgactaa tgttaccaaa aaagataaag taacttttaa
    12361 ccttatttat cgtcgccact caacatgttt ttaacacctt ctacagcacc ttctacagcg
    12421 tctttagcgt cttcggcaac ttcttttact ttagaaacaa ctttttcagc tgctccttct
    12481 ttttccattt tttcatcacc gatggctttc ccaacacctt ctttaataga acctttagct
    12541 tgatttaatt tttcttctac tgacatgata atttctcctg ttatatttat tttatcttag
    12601 tttacacgag tgttttcgtg agatactaca ccatttgctg cacctttaac agcttctaca
    12661 ccttcgctaa ctttttcttg aacagttgag aaaccagatc caagaccaga tttagctttt
    12721 tcgaattgtt ctgaagtgaa ttctcctgtt gattcagcaa cgtcagatac gcgatcttga
    12781 aggcttactg agtctgcttc atgctgttct ttagttttga tgtcgacaac gtttacgttg
    12841 atttcaacaa tttccaagtc agtcatttta gcaacttctg aagatacgat ttctctgatt
    12901 tctgaatata aagctggaac atttttttgg tactcaacaa taacgtttaa gtcaactgca
    12961 acttgtgttt taccaacttc tacgttaaca ccacttgtta cgtcatcgct gttaacgatt
    13021 ttttctttaa gatttgagaa gaaaccacca tcgattccca aaagacctga aacgttttct
    13081 agtgaaagac caatgatttt ttggataact ttatcttcgt aagtaagttc ccctttgatt
    13141 tcgtgagcta caacagtagc atctttcttt tctacgttag tgtttgtgtt tttttcgttt
    13201 gacatatgaa actcctttaa ttaaattagt aatttttatt tttttattta tctatatagt
    13261 ttttttcgat atataatcca gctgcaactc ccagtgctcc taaaatcaat acaaatattg
    13321 ttttgaagaa gccaaaggag acaatcaaac aagccagaaa tacgcctatg agaccagcga
    13381 taattggata tcgatattgt ttaagccatt ccatttttta cttccttact tcacacgact
    13441 aacagtcttt ttgttttgag gttttggttg gtaatttttt actgcaactt caagttttac
    13501 ttgacgctca ataccaaaaa actgcttcaa tccattagtt atttcatttt gaattatttg
    13561 gcatctgtca gcgatgttgt ctgaaggaag aattttacct tctacatgaa cgaaacattt
    13621 attttttcgt aaatttacat gaacagttgg gttcttgatc aatctatgat cactcaccaa
    13681 acttctaaca aaaccttcga ttgccgaatt ctttaatttt aatgtatcgt ttttagtttc
    13741 aagttggatt tccaagtaac gtttaggata aaacatcaca actaaaatgg ataataaaac
    13801 taaagttgat agaaccactg tcccccaaaa gacatatcta gcaagataga attcaactac
    13861 ggagttctgt ctccagctaa gtagatgaat acctagatca ctaacttgat gataatctat
    13921 caaaacagga aggaaaattg tcaagattaa aatacagaaa ataatgaaac atattttctt
    13981 tgcttttgac atatacaatc ctttcgattt aacatttaaa ctatttttcc cgtcattgat
    14041 ttaaaaatag tttgctaact ttactggcga aaagtttact ttctacctgt aaagaatgac
    14101 actacagtaa tcacaatcgc tgcacctaca atagatggga gcaaagccat ttcagcgatt
    14161 gatggacccc aactacctaa aagagattgt cctgcatatg ccccgactaa accagcgaat
    14221 acatttgcga tgattcccat agaactactt ttttagtgat tttacctgcc aagagaccaa
    14281 taagacctcc agcaataatt gatcacaaca ctgcctaccc tcctttttta ttttaatatc
    14341 aaaaaatcaa ttt
//
LOCUS       DAVKLX010000050        14301 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKLX010000050 DAVKLX010000000
VERSION     DAVKLX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14301
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.050"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(67..1356)
                     /locus_tag="VW325_001714"
     CDS             complement(67..1356)
                     /locus_tag="VW325_001714"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9735059.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1434..2681)
                     /locus_tag="VW325_001715"
     CDS             complement(1434..2681)
                     /locus_tag="VW325_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU9735060.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAVIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(2872..3723)
                     /gene="rgg"
                     /locus_tag="VW325_001716"
     CDS             complement(2872..3723)
                     /gene="rgg"
                     /locus_tag="VW325_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9735061.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            3847..4188
                     /locus_tag="VW325_001717"
     CDS             3847..4188
                     /locus_tag="VW325_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9735062.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            complement(4397..6100)
                     /gene="ilvD"
                     /locus_tag="VW325_001718"
     CDS             complement(4397..6100)
                     /gene="ilvD"
                     /locus_tag="VW325_001718"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9735063.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAEATLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(6333..7265)
                     /locus_tag="VW325_001719"
     CDS             complement(6333..7265)
                     /locus_tag="VW325_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9735064.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFSSRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(7262..8119)
                     /locus_tag="VW325_001720"
     CDS             complement(7262..8119)
                     /locus_tag="VW325_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9735065.1"
                     /translation="MILSENREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(8123..9469)
                     /locus_tag="VW325_001721"
     CDS             complement(8123..9469)
                     /locus_tag="VW325_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9735066.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(9482..9766)
                     /locus_tag="VW325_001722"
     CDS             complement(9482..9766)
                     /locus_tag="VW325_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9735067.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(9770..11800)
                     /locus_tag="VW325_001723"
     CDS             complement(9770..11800)
                     /locus_tag="VW325_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9735068.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            11991..12998
                     /locus_tag="VW325_001724"
     CDS             11991..12998
                     /locus_tag="VW325_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9735069.1"
                     /translation="MTEKLINSKPNGVFALILIELTIILGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            13001..13201
                     /locus_tag="VW325_001725"
     CDS             13001..13201
                     /locus_tag="VW325_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9735070.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            13353..13535
                     /gene="rpmF"
                     /locus_tag="VW325_001726"
     CDS             13353..13535
                     /gene="rpmF"
                     /locus_tag="VW325_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9735071.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            13551..13700
                     /gene="rpmG"
                     /locus_tag="VW325_001727"
     CDS             13551..13700
                     /gene="rpmG"
                     /locus_tag="VW325_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9735072.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(14192..>14301)
                     /locus_tag="VW325_001728"
     CDS             complement(14192..>14301)
                     /locus_tag="VW325_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9735073.1"
                     /translation="TVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         4607 a         3030 c         2452 g         4212 t
ORIGIN      
        1 tatccacaac ctcaaaacag tgttttgagc aacctgcggc tagcttccta gtttgctctt
       61 tgattttcat tgagtataaa atcctagttt ttcaaagatt tctgagaagt tttggctgat
      121 tgtctcaagt gacacttgca cttcttctcg ggtttggttg ttcttgaccg tcacttgtcc
      181 gctttcgact tcgctctctc ctagggtgat gagggtctta gccgcaaaga catctgctga
      241 cttgaactga gctttgagct tacggttgag gtaatcacgc tctgctttga aaccttgttg
      301 gcgaagagct tggactagtt ctagggcttt gacatttgct ccatcaccca agactgcgat
      361 atagacatct agggcgtttt cgatagggag ggccacgcct tgtttttcaa ggatgagaag
      421 caggcgctct acaccaagcc caaaaccaaa tccagcagtt tcagggcctc caaagtaagc
      481 aaccaaacca tcgtagcgac cacccgcaca gactgtcaag tcattgccct caatctctgt
      541 gataaactcg aaaatggtgt ggttgtagta gtccagacca cgcaccatat tggtatcgat
      601 gatgtaatct actccaagat tttccaacat ctgacgcaca gcatcaaaat gagtttggct
      661 ttcttcatca agaaagtcca agatagacgg cgcattctct actgccacct tgtcttcttt
      721 ttccttagag tccaagacac gaagaggatt ttcctccaag cgacgttggc tatccttaga
      781 caaggtctcc ttgagcggtg tcaaatagtc aatcaaggct tggcggtagg ctgcacggct
      841 ctcaggattt ccaagagtgt tgaggtgcaa tttgacacct tgaataccga tttccttcaa
      901 aaaatgggct gccatagcga ttgtttccac atcggtagct ggattgctag agccaaaaca
      961 ctcaacacca atctggtgga attggcgcaa gcgccctgcc tgtggacgct cataacggaa
     1021 cataggtccc atatagtaga acttgcttgg cttttgcact tctggggcga aaagtttatt
     1081 ttccacatag gaacggacaa cgggtgcagt tccttctgga cggagggtaa tatgacggtc
     1141 acccttgtca taaaaatcgt acatttcctt ggttacgata tccgttgtat ctccgacaga
     1201 gcgactgata acctcgtaat gctcaaaaat aggcgtgcgc acttctgcat agttatagcg
     1261 cttgaaaatc tcacgggcaa agccctcaac gtactgccac ttagcagact cagcaggtaa
     1321 aatatcctgc gttccttttg gtttttgtaa tttcataggg aatcctcttt aaacttaata
     1381 gtcttatttt accacaaata aagggattaa aacagtgaga aaaaaattag gatttagata
     1441 tcatttttga gattaagaat tgtcaaaaaa atagctagca aggaaagacc aacaaatagc
     1501 atccaagtca actgtatatt ccatacggct actagtgaaa aacaagctgt tcccacaggt
     1561 atggataagg taaacaatag acctaaaaaa ttactagtac gagctagaac ctctggagct
     1621 agatttttca tgagcatggc actaatcttt ggttgaactt taccagacac atacagagta
     1681 aagaagagaa atagcaaacc aagcacgact tgattgaata aattagccaa accaactaga
     1741 ctaagtccta cggtcgccca catcatcaat ctaggcaagg actgcttccc aaaataatca
     1801 ttgcccgtaa ggctactgat gataactgct actaaaacac agaattgatt gataaatagt
     1861 gcctctgtat aagaaaaatt caagagagaa tggctcaaaa agaagatatt ataaattcca
     1921 cccaaagcgc cacccaagga attaataagc aagacagcaa agagcataaa accaaagttt
     1981 ttctgtccac ttttaagaaa aacgagacgt aaatttcggt aaattgttag gaactggtct
     2041 ttgatagaaa gcttctcatt ttttaagttt tcaccatcag cagatgacat tgacaggctc
     2101 aatttgcttt ttcctaaaaa gaggatagtg gctgatacta ggaaaaagca ggcattgatt
     2161 cccgcaacga gagaaaaatt gttgaccgat agagctaaga gccagactcc gaaagcttga
     2221 ccaccaatag ctgaaatata ggtgatgaac tgtgaaaaag aataagcctc catcagatca
     2281 tcttcagcta ctttttcctt aataagaggc atacgcaggc cacctgcaaa atcactgatg
     2341 atatcactaa tgacattgat caaacacagg ctagaaaagg caaagagact agcttgctga
     2401 acaactaggg ctgctagaaa aaatagaacc gcctgaaaca aaccgctata gaccatccat
     2461 ttgaccttgt ccctcgtgta atctgcccga atccctgcaa aaactgtaaa gagggtcgga
     2521 agaatcatga caatattcgc catagcaaca gcaaaagatg cttgtgacaa ggtcgatgca
     2581 tagacgataa agaccaggtt gaaaatcgaa gcaccaaaag cattgaagaa gcgtgataaa
     2641 gtcaaaattc tataagctgg atttctaaac aatagtttca tagataatct ccctccttgt
     2701 tctaagttct acatcaagaa tagctttagt atagcactta tgaatttgcg aaacgattct
     2761 tttgaatata tgcaacaaac aaaatgagca aaaaaacgat agaacttttg taatgacctg
     2821 agagagggca acagttctat cgcttcaaat tttttcttgg tttgcagata ttcaacattc
     2881 ggtcgtattt ctatagtatt cggcagattt attacagcca agcatctcaa aaatacggac
     2941 agcatcctcc atctttttct ggccttcctt gactctacct tgcttgctat caaggagacc
     3001 ttctgcccac agataaacaa ttcggaaata ggtctcattt tccttgtaga aatgctcttc
     3061 gataacacgt ttaaaataat aggcattggt aaattcttca cactcaatac tagctaaaaa
     3121 gccattcaat agtatagtat gaaaaaggtt tcgattgcca gacatttcca ttagaaaatc
     3181 agatttacgt accatttctc gtacatatct agtaaaaaga gaaacagata aaaatggaga
     3241 actgactgaa aataaattga gttcatagat tccccagatc tcggtagaaa acaaataatc
     3301 atgaaggact tttccttcct ctgctgttaa ctctaccctt tcatccatgc tcttcatata
     3361 agacttgatc ataatggcat ttagaatatg tttctgtttg ttgtgagaat gggcatgctt
     3421 ttgatactcc ctgcgatata agtcctcaag aggtgctata ttctttggtt ccaagacatc
     3481 tgtaatttct tttctcaact cagaatctgt atcatactgg aaacctctcg ccagaaagag
     3541 gatctcctcc acactggcag atatattttc cagagcaaat agaaactttt ccaccgaaag
     3601 ctcactctga cctgtttcaa aacgggacaa catagacggc gaaaattgtc ctccggttgc
     3661 ttgtctcagt gagatatttc ttgactctcg taattgtcta aagacttttc caatctgctc
     3721 catagacttc cccttgattc cgtattttct tcattttatc atatttttca gaaaattcat
     3781 caaaaacttg ccaaattgtc agaattatga gaaaatagag gatatttatc acgtggaggg
     3841 actgctatga gagacgatat caaaatcaat gaccgtgctt tggccttgca agaccaaatt
     3901 atcgaaaaac tagagaaagt ttttgataca gatgtggaat tggatgttta caatctaggt
     3961 ctgatttatg aaatcaatct ggatgaaacg gggctctgca agattgtcat gaccttcacc
     4021 gatactgcct gtgattgcgc cgaaagcctg cctattgaaa tcgtggcagg tttgaaacaa
     4081 atcgagggta tcaaggatat caaggttgaa gttacctggt cgcctgcctg gaaaatcaca
     4141 cgaatcagtc gctatggccg tattgccctt ggactaccac ctcgttaagc aggtcaatca
     4201 cttttaaaga tgaaaaaagc aagccgaaaa tggcttgctt tttgatactc aatgaaaatc
     4261 aaagggcaaa ctagaaaact agccgcaggt tgctcaaaac actgttttga ggttgtagat
     4321 agaactgacg aagtcagtaa ccatacctac ggcaaggcga cgttgacgtg atttgaagag
     4381 attttcgagt atgagtttat tttttacctg acttgtccat attccagaag tctgtcacgg
     4441 ctccgcgtga agcagatgat acgatgtggg catatttacc gagtacacca cggctgtaaa
     4501 gtggtggcaa ggttgcttct gccttgcgtt tttcaagttc ttcttcggat acggccatgg
     4561 aaatttcttt ggtatcttgg tcaaccgtaa cgatatcgcc ggtacggaga taggcaattg
     4621 gtccaccatc ctgagcttca ggagcgatat gtccaacaac cagaccataa gtaccaccag
     4681 agaaacgtcc gtccgtcaag agggccacct tatctccctg acctttacca acaatcattg
     4741 aagaaagtga tagcatctca ggcataccag gaccaccttt aggtccaaca aaacgaacaa
     4801 cgactacatc gccatcaacg atttcatctg tcagaacggc ctgaatcgca tcttcttctg
     4861 agtcaaagac cttagctggc ccaacgtgac gacgcacttt aacacctgat accttggcaa
     4921 ctgcaccgtc aggagcaagg ttcccgttca agatgataag cggaccatcc gcacgttttg
     4981 gattttcaag tggcatgata actttttggc ctggagtcaa gtctgcaaag tcagccaagt
     5041 tttcagctac agtcttacca gtacatgtga tgcgatctcc gtgaaggaaa ccatttgcca
     5101 acaagtactt cataaccgca gggacaccac cgacttcgta gaggtcttgg aagacatact
     5161 gaccagatgg tttcaagtcg gccaagtgag gcacacgttc ttgaatcgta ttgaagtcct
     5221 caagtgacaa gtcaacattt gcggcatggg caatggcgag caagtgaaga gtggcgtttg
     5281 tagaaccacc gagagccatc gttacagtaa tagcatcttc aaaggcttca cgagtcaaga
     5341 tatctgatgg tttgagacca agttccaaca tcttaacaac agcacgtcct gctgcttcga
     5401 tatcttcttt cttatcagct gattcagctg ggtgagagga tgaccctggc aaactcatcc
     5461 ctagaacttc gatagcagtt gccatggtat tagcagtata cataccacca caaccaccag
     5521 ggccagggca ggcattacat tcaagacgtt tcacgtcctc agctgtcatg tcaccgtggt
     5581 tccattttcc gataccttca aagacagaaa ccaagtcgat atctttacca tcaagatttc
     5641 ccggtgcaat agttccacca taggcgaaaa tagctgggat atccatatta gcaatagcaa
     5701 tcatagatcc aggcatgttc ttgtcacagc caccgatagc gacgaaggca tccacgttgt
     5761 gaccactcat agccgcctcg atggagtccg cgatgatgtc acgagatgtt agagagaaac
     5821 gcataccagg cgttcccata gcgatcccgt ccgctacggt aatggttcca aactgtacag
     5881 gccaagcgcc tgcagatttg acaccttctt tagccagttt cccgaaatca tgcaagtgaa
     5941 tgttacatgg tgtattttcc gcccaagtcg aaatcactcc cacaatcgat gtttcaaagt
     6001 ccttatctgt cataccagtc gcacgaagca tagcacggtt aggtgattta accatgctgt
     6061 catagatgct actgcggtga cgtttatcta attcagtcat cttatccctc ccatttcagt
     6121 ttttactatt atagcacaat ttaaaggcaa tgaacagaat aaaattcttg aattttcaga
     6181 aaattctata cgcatgtgaa atatttaaaa ttaaaaacaa caaagcggat tagtgcactt
     6241 tctgatgacc agaatatgct ttttaatccg ctttctttaa ataacgtact gtaattttta
     6301 cagaaattct ttcagataag tgtatttaac atctatcttg cattataaat ttctagaacc
     6361 ttctctttta tattcgattc actcaaacca tactcattaa gaagataatc cattttccct
     6421 acttgaccaa atctttcttg aacacccatc cgatgaattt ttgttattcc atcatcagag
     6481 aataattcac ataaagcact gccaattcca cctatctgat tgtggttttc tacagtaaat
     6541 atagtttttc cacttaacat tgtttttatc tgttctggta tcggtttgat tctaaataaa
     6601 tctatcacac ctactgaata acctaattta gacagttcat ctgcaactcg aatacttgga
     6661 gcaaccatta taccagaagc aacgattaca agatcttcac catgccttaa ctcaatgtag
     6721 cctttagaaa aatcttctcc accttgatac acaggaactg gagcttttct aattgttcga
     6781 atatatttta gtccttttaa gtctaatgtc tggttcaata tttcacgaaa ttggatatca
     6841 tcagttgctt caaaaatgat cgatttagga attaaacgta acaatccaat ttcttcaaat
     6901 ggcatatgtg tcccaccatt catttctgca gttactcctg catctgatcc aatcacagtg
     6961 gcatccaatt gtgcgtatcc aagagaaata aataattgat caaatactct tcgtgaagaa
     7021 aaaggaccaa atgtatgaag ataaggtcta aacccctgaa tagacaagcc tgctgcaagg
     7081 ccgaccattt ctgcttccat aatcccaaca ttcacataac ggtctccaaa gtccttttca
     7141 agattatgag tagccatcga acttgacaga tcggcttcta agactactat atcagaatca
     7201 ctttgattag cctctaaaag gaagtctcta tatacatgcc gtaattcttt cgtacttctc
     7261 atcattctgt ttcctccaat tcctgactta atctttctac aactgaagtt aacatttgtc
     7321 tctcctctac agtagggcga agatgatgat tggatttcat ttcttccagc tcttgaaccc
     7381 cttgaccttt aatagtatct aatacaatgc acttaggtga tgaattattt gactgtttta
     7441 attggacaat cccttcataa atttctctaa tatctgaacc cttgacccta atggattcaa
     7501 atccaaatgc tgaaaatttt tctacgaaat cacctggatt acaaatatcc tttgtaaaac
     7561 catctaattg ttttttgttg tcatcaacaa atacaattaa gttggataac tgttgatgag
     7621 aagcaaactg tatagcctcc caacattgtc cctcatttaa ctcaccatct ccaacaatag
     7681 cataagtata aaagggactc tttcttattc tctgaccata tgcaagtcca gttgcaacac
     7741 taattccttg tcctaaagag cccgttgtca tatctatgcc tggcgttaga tttctatcag
     7801 gatgagacgg taatttggtt ccatttgtat ttaaagaata taagaattct ttgtcaaaga
     7861 aaccattcaa atagagtgta ctgtatagag ctggtcctcc atgacctttt gataatatga
     7921 aataatctct atctcgtgct gcaaatattt ctggagtcat tggcattatt tcaccataaa
     7981 gcactgctaa aacttctacg atagacagac ttcctccgta atgtccgaat ccaagatgat
     8041 tcaatgttct aagagtattt aatcggatgt tagtcgcaaa ttttcttaac tcatcttctc
     8101 tattttcact taaaatcatc ccttactcct cctttgcaga tggcttttta ataaaggata
     8161 ctccaaacat aactgctaga ataagaacaa gaccaatcac aatgcctgct tgtgaaccaa
     8221 attgatttaa cattcctaaa ataattcctg atagaccaaa atctgcatct gagaaagttg
     8281 atccttggaa accaagtcct cccaaaactg gcattaaaaa gactggaaga aaactgatta
     8341 aaataccttg taaaaatgct ccaatagtgg ctccacgaac accaccagat gcattcccaa
     8401 tgacacctgc agtcgctcca cagaagaaat gaggcacaac gcctggtaag ataacaaccg
     8461 ttcctgaagc aatcataatt gccatactta ctaaaccacc aataaaacta gagataaatc
     8521 caattagaac tgcattgggt gcataagtat aaacaatcgg acaatccaaa gcaggttttg
     8581 aattaggtac aagacgctct gaaatacctt taaaggctgg aacaatttca cccaaaataa
     8641 ggcgaacacc tgctaaaata acaaataccc ctgctgcaaa ttgacctgct aattgtaaag
     8701 cataaactag accacttgta ccactactga tttctttttc tatatattct gaccctgcaa
     8761 agatagctac aataatgtaa ataactgcca tggataaagt aatactaaca gtgctatcac
     8821 gtaaaaaagc taaactcttt ggaaatttaa tgtcctctgt tgattttgat ttgtcaccga
     8881 taaggctacc agtaaaacca ctcaaccaat atcccaaaga actgaaatga cctaaagcca
     8941 ccttgtcatt tccagttaat tgaaccatat atttttgcac aaatgctggg gaaatactca
     9001 taataatacc gagtgctaat cctcctagta agataagagg caagctagta aagccagcaa
     9061 ctgataaaat gaccgcaatc atacacgcca tatatagagt gtggtgccct gttaaaaaga
     9121 tatatttaaa tcgagtaaaa cgagcgatta agatattgaa caccattcct gcaaacataa
     9181 tcattgcagt agctgagcca tatgttgtta aagctacagc tacaattgct tcattattcg
     9241 gcacaacgcc agataaatga aaagcatgct caaacatggt accaaatgga tttaaagaat
     9301 tttgtacaat tcctgcacca ccagatacaa ctaagaaacc aacaaaggtc ttaattccac
     9361 ctttaataat atcaggtaat ttcttcttct gaagaactaa tcctaaaatt gcaattaaag
     9421 ctactaaaat agctggtgta ctaacaatat ccaatatgaa cttcatcatg acgctagcct
     9481 cctatataag tcctttttct tcacaaagtt tagtaattaa ttctcgtagt tcatccatat
     9541 caataatact atttaagata cgaacatctc caagatgact agctgaatca gctagatcac
     9601 gaccaacaat ccaaatatca gctgcatttg gatctgctcc acctaaatca taatgttcaa
     9661 cttctacatc cgaaacattc aaatcactca atacagattc aatattcatc tgtaccataa
     9721 aacttgaacc taatcctgaa ccacaagctg taccaatttt taacattatc taatcctcct
     9781 gtttaattat cattttaata tcatcatagt tttttgatga tattaaagtt tgaacatgat
     9841 ttttatctct taaaattgtt gttaaatgtg acaaagcctt taaatgactc tcattatcaa
     9901 tggctgcaat acaaatcaac aatcttacct cttgttctgg attatccaat aaataaatcg
     9961 gttcttccaa aactaacatt gacattccta tttcattcac accttcatct ggccgagcgt
    10021 gaggaattgc tactcccttc cctaaattaa taaaaggtcc aaactcttct actttttgaa
    10081 tcattgcctc agggtagttc tccgttatct tatcttgatc caaaagcggt ttagctgcta
    10141 aacgaatcgc ctccttccat cctaattttt gcgaactaac ctgataggtt tctttggtaa
    10201 taagttgttc tagcactggt acaatttcct ttctatcatt tttttgataa agataattct
    10261 ttaacgccaa tcttaattcc aattcttgtg taataattcc atatcttttg acaatattca
    10321 ggatttgttc aatctcaaaa tctccatact ctaaattcgg aaaatctttt aacactagtt
    10381 ctactagttg tattgcttgc tcttcagtca tcataaccga aactagataa tttggctttt
    10441 ctgtctccac ctttatggta gaaaaaacca tatcatagtc actactagct ttcacctgta
    10501 aatcatcaat ctttgaggtt cctataaact caatttgagg aaataatgct aatagattct
    10561 cttttaacat caatgaagaa ctaacaccat taggacaaat gattgctgct ttataccatt
    10621 tttgaggcaa agtatctgct ttctttaaat aacctccgaa atggataaca aaatatgctg
    10681 tttcactatc aggtatggga ttgtcaatag cgtccatcaa gggcatcaaa gaatctttga
    10741 ctaattcaaa taaatcagga taatgttctt taacatgcaa tacatattca tttgaactag
    10801 gtaggccgaa ctttaatcta tagtaagccg gtataaggtg gcggcgaaga ttttctctca
    10861 atccttccct ttgtttaaaa tgtaacaaag aaatatcttc cattctactt ataatagcct
    10921 ctgttaattg attaaagtaa accggagcaa catctacttc accttcaaag caacttgata
    10981 ataaaactgt gatatagcga taatcatcct cagaaaacac cgtatctata attcccaaat
    11041 caaccactgt atccaataaa atagtggtta tatcttgaat aacaggagat actaatgtct
    11101 ctgaaagaca tactctttca acatcccttt gatacctaca cagaatgaat actaaaccga
    11161 aaaggtaaac ttttaattga ttaacaatag gtactagctg tagcttctca taataatctt
    11221 taactacctg atttatcaaa tcataagtta atgaataccc ccaactggat aaaacataat
    11281 ccaaacccca aatccctatg gaggattcca gcaactcact aaccatttga aaagctaagc
    11341 ggtgcttatt ccactctgaa ccgtgtaaag tataaccttt tgctctactg taccctagct
    11401 ccaaatcatt atctaacata atctttctta atgattgaat atcagataag gttgtattct
    11461 tacttacttt caaaaagtct tggtaatgat tattcgatat aaaatctaat cggcaaaaag
    11521 tgtaaagata gattaaagct aagcgagtcg actttggtaa aaccaattca tccgacttaa
    11581 taatatctgt caaagactgc ttcgtacgat ttgataaact atagcgacct tgttttttat
    11641 ccagcactat ccctttatta gctagataag gcactaaata atctattcct tctttgactt
    11701 cctttatagg taagctcacc ttaacagata attcatataa tgatagctca caatgatcca
    11761 tcaaagtcat caaaataact agtgctctat aatcaaacat ttctcttcct ttctaactac
    11821 aattatagtg taaaaattat aagaataaaa gagtatttga gcacaatata atctcacctt
    11881 ttaaaagaaa ttattgggct gaaagttttt agtgattatt ttcaactttc tattgacttt
    11941 taaaaacaaa gtgatatcat ttagatatca aataatggga ggtcattctt atgactgaaa
    12001 aactaatcaa ttcaaaacca aatggtgtat tcgcattgat tctcattgag ttgacaatca
    12061 tacttggtat ctttatattt ataatgggtg ttggttcgga aaacattttt ggaattatta
    12121 tcggaccttt actaatcgta attgcaggtc tagctcatgc tggtttaaaa gttgtcaaac
    12181 ctcaggaagc tctggttctg acactctttg gtaattatac aggtaccatc aaagaacctg
    12241 gtttttactt tgtcaatccc ttcagcgtag cagtcaaccc tgcaaaccac actcgacttg
    12301 gacaaagtgg tgatgttagc acaaaatctc cttttttagg agctaaatca tcaaatgaca
    12361 atgatgtaaa tcttgaaatt ggcaagaaac acatttccct caaagtcatg accttgagca
    12421 attctcgtca aaaaatcaat gattgcttag gaaaccctgt agaaatcggt atcgcggtaa
    12481 cttggagagt tgtcgatacc gctaaggcag tcttcaacgt tgataactac aaagaatatc
    12541 tttcattgca atgtgatagc gccctccgta atattgtccg catctatcct tacgatgtgt
    12601 ctcctaatgt ggatactacg ggtgacgggc aagcagatga aggtagtctc cgtggctcta
    12661 gcgaaattgt tgctaaccgt attcgtgaag aaatccaaag tcgtgttgag gatgctggct
    12721 tggaaatcct tgaagcacgt atcacttacc tagcttatgc tccagaaatt gctgccgtta
    12781 tgcttcaacg ccaacaagca tctgccatta ttgatgcacg gaagatgatt gtagatggtg
    12841 ctgtaggaat ggttgaaatg gcactagaac ggctcaatga aggggaattg gtagaacttg
    12901 acgaagaacg aaaagctgcc atggtttcaa atctccttgt cgttctttgt ggcaatcatg
    12961 atgcacaacc aattgtcaac acaggaagtc tttactaaag atggctgaag ctaaaaaaaa
    13021 gcagatcccc cttcgactct caacaaagtt atacgctgca ctcgcatcat gggcagaaga
    13081 cgactttcgt tcagtcaatg ggcaaatcga atatctcctc acagagtgtg tcaagcaacg
    13141 gaaaaaagac ggaaaatacg tatcagaaac cattgacgaa ccatttgaga ttgatattta
    13201 acatcttctc ctgtctgaca agtcggacag gagaattttt tcatcttttt ttgtcaagta
    13261 actttacttt acaaaaaaaa tgtgttatcc tagtatggtt gatgaaaatc agtagattga
    13321 atcgaatata aatacctaaa ggagaaatca aaatggcagt acctgcacgt cgcacttcaa
    13381 aagcgaagaa aaacaaacgt cgtacacact acaaagtaac agctccatct gtaaactttg
    13441 acgaaactac tggagattac tcacgttctc accgtgtatc acttaaagga tactacaaag
    13501 gacgtaaaat cgctaaagct gcatcagctg aataatagaa gggagatacc atgcgcgtaa
    13561 atattacact tgaacacaaa gaatctggtg aacgcttgta ccttacttct aaaaacaaac
    13621 gtaacactcc agaccgcctt caattgaaga aatactcacc aaaacttcgc aaacacgttg
    13681 tgtttacaga agtgaagtaa gcattcaata cgaatcaata cagaagaaaa acgcttgatc
    13741 agcgtttttt ctttttgtta atttcactat attgcaaggg aaatcattcg aatgactacc
    13801 tttttcacta ccttttttga aataagaata cggattcgga cgattaaaag taagagaaac
    13861 tcaaatgtaa gttggggttc cttttttgta tatttttcaa ataacgcctc tacactattt
    13921 gtagcaaatt caccaactac agttgtatct tagttaaaat aagttagaat atgtaagtga
    13981 gtaccagata taccaagaca tcgtcaccat ctaaggtata ttcaaaatac aaaagttgac
    14041 caactagatt tctgaatatc cttatatatc cattcttaaa attggtttaa atagcgtagt
    14101 cttttaaact agttttgaga atccaaaaaa tcttcctaca tatgtaagaa gattttttag
    14161 ttcagaatga ttagatttag ctaatggata cctatcctac ccactcaccg ttagcattga
    14221 ctcgatagcc atcgggtgta gtcgtgttta ctaataaatc tcctgaatta taggtatagt
    14281 accatttacc agaaactgta a
//
LOCUS       DAVKLX010000051        14045 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKLX010000051 DAVKLX010000000
VERSION     DAVKLX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14045
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.051"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(23..874)
                     /locus_tag="VW325_001729"
     CDS             complement(23..874)
                     /locus_tag="VW325_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9735074.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
     gene            1201..1593
                     /locus_tag="VW325_001730"
     CDS             1201..1593
                     /locus_tag="VW325_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735075.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAVDMARQNWWDLSAAISGSWSPN"
     gene            1615..2406
                     /locus_tag="VW325_001731"
     CDS             1615..2406
                     /locus_tag="VW325_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735076.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            2399..3040
                     /locus_tag="VW325_001732"
     CDS             2399..3040
                     /locus_tag="VW325_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735077.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLILSRLEEKFKKG"
     gene            3044..3694
                     /locus_tag="VW325_001733"
     CDS             3044..3694
                     /locus_tag="VW325_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9735078.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            3705..3992
                     /locus_tag="VW325_001734"
     CDS             3705..3992
                     /locus_tag="VW325_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735079.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            4138..6651
                     /gene="secA"
                     /locus_tag="VW325_001735"
     CDS             4138..6651
                     /gene="secA"
                     /locus_tag="VW325_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9735080.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            6732..7763
                     /locus_tag="VW325_001736"
     CDS             6732..7763
                     /locus_tag="VW325_001736"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9735081.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            7765..8796
                     /locus_tag="VW325_001737"
     CDS             7765..8796
                     /locus_tag="VW325_001737"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9735082.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            8835..9203
                     /locus_tag="VW325_001738"
     CDS             8835..9203
                     /locus_tag="VW325_001738"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9735083.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            9193..10296
                     /locus_tag="VW325_001739"
     CDS             9193..10296
                     /locus_tag="VW325_001739"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9735084.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            10315..12330
                     /gene="recG"
                     /locus_tag="VW325_001740"
     CDS             10315..12330
                     /gene="recG"
                     /locus_tag="VW325_001740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9735085.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWCMIALHLEKKEHLD"
     gene            12646..13626
                     /locus_tag="VW325_001741"
     CDS             12646..13626
                     /locus_tag="VW325_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9735086.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT         4138 a         2552 c         3128 g         4227 t
ORIGIN      
        1 aaatgatgag agttattaca aattataggt ttgaaaaatc attaatttct ttttcgagta
       61 ttttaactaa tgcctcttct ttggttagtc gtaataaagt tattccttta tcaatcaaat
      121 cggagttatg ttgtagtatt cccaagcgta cttgagagaa tccaagaata tcgtatattt
      181 ttaattcttc tgctaatacc aaaagttgta gggtgattgt ttcacattct ttgaataaat
      241 gatgttgaaa atatacagtg gagagattgg ctaaaaaaga tgataaaaat gcttttgttt
      301 ctcggaagta tttatatttc tcgatggttt tcataattct atcaataaga aggggaagat
      361 tttcagaagg aaagaaaaag agaatggttc caagcaattt caaatcacta atgtaccatg
      421 tatccatagg ttcaagatag tcccaaattt cttcatacaa atctttcaag acgtggttgt
      481 ttttgaatcc tttagttcgt aactctgtga caatttttgt atgacgatag atattttgaa
      541 taggaacatc gtgatgtgtc ttaagatatt tttgacattt ctcagtgagt tcaaccattt
      601 ttctagtatc ttgaaaagta gacggatttt ggctctctac aataatatct cgccttttac
      661 taggatggta ttcgttgcat atatacttga attctgccaa gctcatatca atttgttcaa
      721 gaagaaatac catgttttca aaactgggaa taactttacc gtgttcaata tttgttagag
      781 ttgttcgatg tatgacttgt ccacatacat cttgttgggt caatcctttt gactttcgaa
      841 tttctttaaa aatttgtcca taatcccatc tcatattgtt cctcctaggt aaatgtgaat
      901 attttctaca tttataaaaa acgtagaaaa aggtggtatt attatatcaa aaaggaggga
      961 aaaagtcatg ggctttaaaa aatatttgaa gaatttaccg aaaaactctg gatttttgat
     1021 ttggagttgg attcaactta tctggtttga aacatggttt tggggataaa gaaattagag
     1081 tctatacata ataagtaaag gaggaatttg tatgatatac catcgtttag aataagtcta
     1141 tatttaaaat agaagttata aggaatcttc ttgaataaaa aatgaaagga aacagaaaaa
     1201 atgaaaatta agaaattatt gaaaatggtt attcctgttt taatgataag tgctgttgga
     1261 actactttcg tagaagcaaa tcagataggt gcttttagta attttgttat tactaccagt
     1321 tataagagaa caggttattt gactaaagaa aatgaaggtg cggaatatat catgaactta
     1381 aatccttgta gaaatttaca tcccatgact gttaagcatc gtatagtaaa ttctaatgga
     1441 gaagctcgta gtggagaatc gttaacaacc tgtggtactc gttcaactca tggtaattgg
     1501 gcaacggttg gctatgttta tgcagtagat atggcaagac aaaattggtg ggatttatca
     1561 gctgccattt caggtagttg gtcaccaaat taattcgtaa agtaggtata atttatgaag
     1621 attcctctct taacttttgc aaggcataaa tttgtttatg tcttgcttac tttgcttttt
     1681 cttgctttgg tttatcgtga tgttttgatg acttatttct tttttgatat tcatgcgccc
     1741 gatctagcta aattcgatgg acaagcaatt aaaaatgact tattaaaatc agcattagat
     1801 tttcgtattc tccagttcaa tctaggtttt tatcaatcat ttattattcc aatcatcatt
     1861 gttttgctag gttttcaata tattgagctg aaaaataaag ttttacgatt gagtattgga
     1921 agagaagtga gctatcaagg gttaaaaaga aagttgactt tgcaagttgc aagtatccct
     1981 tgtttgatat atttagtgac tgtgctgata attgcaatta taacctattt ctttgggact
     2041 ttttctcctc ttgaatggaa ttctctattt tctgatggga gtggtttaca aagactccta
     2101 gatggagaga taaaaagcta tttgttcttt acttgtgtcc tattaatcgg tattttcatc
     2161 aatgcaatct attttttaca aatagttgat tatgtgggga atgtgactcg ttcggcaatc
     2221 acctatttga tgtttctttg gcttggttct atgctgctct atagtgcctt gccttactat
     2281 atggttccta tgacgagttt gatgcaagct agctatgggg atgtaagttt gatgaaactc
     2341 tttactcctt atatccttta tgttgtccct tatatggtac ttaaaaaata tgaagataat
     2401 gtttaagaat tttaacaata tttggctaaa gagaaagatc gttttactac ttcgtatagt
     2461 tctgatgatg attttgataa actatctatt gtcaacagcg gttcaaaagc aggatgctgt
     2521 tatctttttc aagagagaat tgatttcaat tttttcctat aatgactatt ctgaagcgaa
     2581 tttagaaatc cccaaactct tgttaaacct ttcgcttttc atggtaggat ggctctctgt
     2641 cattttactt gaaagtgatt tggcagacca ttaccatcac ttgattcgct atcaatcaag
     2701 ctcctttttc gattatacaa ggaaacgatt ggttgtcatt tctaaatttt ttactcaaga
     2761 tttgtttgtc tggtttcttg gtttacttcc gctaggaatt catttcaaaa cagtcgcact
     2821 tttcttttta cttgctcagt taatgatgtt gtacttacta ctgtcttatc tgatagcact
     2881 gattagtgcg ggcgctggtt tttctttttt tctctatttt ttggcatttg tgggacaaga
     2941 atggatgatg gatcatattg taacagtgta tttagtactc ttaagcttat tagttatgtt
     3001 gattcttagt cgcttggaag agaaatttaa gaaaggataa acgatgagac ttgaaattat
     3061 aaatggacag aaaatttatg ggaaaagacc tattttaaat cagttgaatt tggtgtttca
     3121 atcaggaaaa atttatggac ttaaaggtga taatggatct ggcaagacgg ttcttttaaa
     3181 gatacttgct ggttatatta agcttgacaa aggaaaagtt cttcaagatg gtaaagttta
     3241 cggggtaaaa aatcattata ttcaggatgc aggaatttta attgaaaaag tcgagttttt
     3301 atctcattta tccctgagag aaaatttgga actgttaagg tatttttcat ctaaagttac
     3361 ggaaaaaaga attgcctatt ggattcaata ctatgattta caggaatttg aagacgttga
     3421 ataccgtcat ttatccttgg gaacaaagca aaaaatggcc ttgattcaag cctttatttc
     3481 ctctccttct atactctttc tcgatgaacc tatgaatgct ttggatgaga aaagtgtgag
     3541 gttaaccaaa caggtcattt tatcttacct gaaaaaagaa aatggtctgg ttatcctgac
     3601 gtcgcacata tcggaagata tttcagacct ttgtacagat gtattagttg tcgaaaatgg
     3661 acatatacaa tatgtaaagg atatacaatc ctaggagatg gcttatggca catctaaaat
     3721 catttattac acgctattcc aaggtttata ttggtttagt tctgctgatc tggctgtctt
     3781 tcttctttat cccttgggat agagcgattc tgggggtaag gattgacatc ttcatcatac
     3841 agaaagtctt gctagttttt ggaattctgt ccattttgat ggccttgctg tccaagaaag
     3901 tcagtctctt tgtttttgga ctgatttgct gtctttctct ttggattaac ttatttatca
     3961 catttgccat tttgccaatt tttggcaatt aaacagtcat aaaagtcgga gaggttagct
     4021 tgaaaactaa cctctttttc cttttcaaaa tggggattct tccttgaaaa taatcagtaa
     4081 ttgtgctaaa attaaaggaa cattctaaaa tattcggaat ttaaagtaag gaaaaacatg
     4141 gctaatattt taaaaacaat tatcgaaaat gataaaggag aaatccgtcg tctggaaaag
     4201 atggctgaca aggttttcaa atacgaagac caaatggctg ctttgactga cgaccaacta
     4261 aaagcaaaaa cagttgaatt taaggaacgt tatcaaaatg gagaatcact ggattcattg
     4321 ctttacgaag catttgcggt tgtccgtgaa ggtgccaaac gtgtcctagg tctcttccct
     4381 tataaggttc aggtcatggg ggggattgtt cttcaccatg gtgacgtgcc agagatgcgt
     4441 acaggggaag ggaaaacctt gactgcgacc atgccggtat acctcaatgc cctttcaggt
     4501 aaaggggttc acgtagttac ggttaatgaa tacctgtcag aacgtgacgc gactgagatg
     4561 ggtgaattgt actcttggct tggtttgtca gtagggatta acttggctac caaatctcca
     4621 atggagaaaa aagaagccta tgagtgtgat attacttact caactaactc agaaatcgga
     4681 tttgactacc ttcgtgacaa catggtcgtt cgcgctgaaa acatggtaca acgtccgctt
     4741 aactatgcct tggtcgatga ggttgactct atcttgattg acgaggctcg tacacctttg
     4801 attgtatcag gtgccaatgc ggttgaaacc agtcagttgt atcacatggc agaccactat
     4861 gtaaaatctt tgaacaaaga tgactacatc atcgatgtgc agtctaagac tattggtttg
     4921 tctgattcag ggattgacag ggctgaaagc tacttcaaac ttgaaaacct ctatgacatc
     4981 gaaaacgtgg ctttgaccca ctttatcgat aacgcccttc gtgccaacta catcatgctt
     5041 ctcgatattg actatgtggt gagcgaagag caagaaatct tgattgtcga ccaatttaca
     5101 ggtcgtacca tggaaggtcg tcgttattct gatggattgc accaagctat tgaagccaaa
     5161 gaaggtgtgc caatccagga tgaaaccaag acatctgcct caatcacgta ccaaaacctt
     5221 ttccgtatgt acaaaaaatt gtctggtatg acgggtacag gtaagactga ggaagaagaa
     5281 tttcgtgaaa tctacaacat tcgtgttatt ccaatcccaa caaaccgtcc tgttcaacgt
     5341 attgaccact cagaccttct ttatgcaagt atcgaatcta agtttaaagc ggttgtcgaa
     5401 gacgttaagg ctcgttacca aaagggtcaa cctgtcttgg ttggtacagt agcggttgaa
     5461 actagtgact acatttctaa gaaattggtt gcagctggtg ttcctcacga agtcttgaat
     5521 gccaaaaacc actatagaga agcccaaatc atcatgaatg ctggtcaacg tggtgccgtt
     5581 accatcgcaa ccaacatggc gggtcgtggt accgacatca agcttggtga aggtgttcgt
     5641 gaacttggag gactttgtgt tattggtaca gaacgtcatg aaagtcgtcg tatcgataac
     5701 cagcttcgtg gacgttcagg tcgtcaagga gatccaggtg agtcacaatt ctacctatct
     5761 cttgaagatg atttgatgaa acgttttggt tctgaacgtt tgaagggaat ctttgaacgc
     5821 ttgaacatgt ctgaagaggc cattgagtct cgcatgttga cgcgtcaggt tgaagcagct
     5881 cagaaacgtg tcgaaggaaa taactacgat acccgtaaac aagtccttca atacgacgat
     5941 gtcatgcgtg aacaacgtga gattatctat actcaacgtt atgatgtcat tactgcagac
     6001 cgtgacttgg cacctgaaat tcaggcaatg atcaaacgca cgattggtcg tgtcgttgac
     6061 ggtcatgcgc gtgctaaaca agatgagaaa ctagaggcaa ttttgaactt cgctaagtac
     6121 aacttgcttc ctgaagattc tattacgatg gaagacttgt caggcttgtc tgataaggcc
     6181 atcaaggaag agcttttcca acgtgccttg aaggtttacg atagtcaggt ttcaaaacta
     6241 cgcgatgaag aagcagttaa agaattccaa aaagttttga ttctacgagt ggtggataac
     6301 aagtggacag atcatatcga tgcccttgat caattgcgta acgcggttgg acttcgtggc
     6361 tatgctcaga acaaccctgt tgttgagtat caggcagaag gtttccgtat gtttaatgat
     6421 atgattggtt cgattgagtt tgatgtgaca cgcttgatga tgaaagcaca aattcatgaa
     6481 caagaaagac cacaggcaga acgtcatatc agtacaacag cgactcgcaa tatcgctgct
     6541 caccaagcaa gtatgccaga agatttggat ttgagccaga ttggacgcaa tgaactttgc
     6601 ccatgtggtt ctggtaagaa atttaaaaac tgtcacggta aaagacaata aaatgagata
     6661 gtttagaggc ggatatcttg tgaaaagtaa atttttactg ggtatccgtt tgctttataa
     6721 ggagatgagt tatggtattt acagcaaaaa gctctaaaat aaatatagaa gaagttcgtg
     6781 ccttgtcaaa attagaaggt caggctttgg agaggaaatc acagcgagat caagagctag
     6841 aagccattat acgtggagaa gaccagcgaa ttctcttggt aatcgggcca tgctcatctg
     6901 acaacgaaga agctgtcctt gaatacgcta agcgtttggc agtcctacaa gaagaagtgg
     6961 cagatcgtat ctttatggtt atgcgtgttt atactgccaa accccgtacc aacggagatg
     7021 gctataaggg cttgattcac cagcctaacg cgacagaagc gcctagtctt atcaatggaa
     7081 tcaaagccgt tcgccatctt cactatcgtg tcatcacaga aacagggatg acaactgctg
     7141 atgaaatgct ttatcctgaa aaccttccgc ttgtagatga tttgatttct tacatggcag
     7201 ttggtgcccg ttcagttgaa gaccagcaac accgctttgt ggcaagtggg gcaggatttt
     7261 ctactggttt taaaaatcca acctctggaa atctcaatgt catgtttaat gggatttatg
     7321 ctgctcaaaa caaacaaagt ttccttttct taggaaaaga agtagaaaca actgggaacc
     7381 cgctttcaca cgctattctt cgtggtgctc ttaatgagta tggaaaaaat attcccaact
     7441 actattatga caatttaatt gataccattg cccagtatga gaaaatgggc ttggaaaatc
     7501 cttttatcat cattgatacc aatcatgaca attctggtaa gcagtatatt gaacagatcc
     7561 gaattgtccg ccagaccttg attaaccgtg cttggaatga aaaaattaag cagttcgttc
     7621 gtggttttat gattgagtct tatctggaag atggtcgaca aaatgagcca gaagtatttg
     7681 gtaagtctat cacagaccct tgcctgggtt gggataacac agaagctctt gtcagagaaa
     7741 tttacaaaac gttaggagaa taagatggca tttattgaaa aaggtcaaga aatcgatatg
     7801 gaagtcatca aggctgaaac ccaattgtct gcagaagcct tgagactcaa ggaaagccgt
     7861 gacagggaat tggcagatat tatttcaggg gaagatgacc gtattctctt ggtgattggt
     7921 ccttgctctt ctgataatga agaggcggtc ttggaatatg ctcgccgttt atccgccttg
     7981 caaaagaagg tagcggacaa gattttcatg gtcatgcgcg tgtatactgc taagcctcgt
     8041 accaatggag acggctataa aggattggtt caccagccag atacttctaa ggctccaagc
     8101 ctgattaacg gcttgcaggc tgtgcgccag ttgcactacc gcgtgattac agagactggt
     8161 ttgacaacgg cagatgagat gctttatccg tcaaatctga tcttggtgga tgacttggtc
     8221 agctaccatg ccgttggagc tcgttctgtg gaagaccaag agcaccgctt tgtggcttct
     8281 gggattgatg caccagtagg gatgaaaaat ccaacctcag gaaatttggg tgttatgttt
     8341 aacgccatct atgctgctca aaacaagcaa accttccttt atcatgggca ggaagttgag
     8401 acatcaggta atcctttggc ccatgttatc ctccgtggag cagtcaacga gtatggcaat
     8461 tatatgccga attactacta tgaaaaccta ctccaagcca ttgaacgata tgaaaccatg
     8521 ggacttgaaa atccttttat cctcattgac accaaccatg ataactcagg caagcaatat
     8581 atggagcaga ttcgaattgt tcgccagacc ttgcagaatc gtgattggaa tgagaaaatt
     8641 aaaaagacgg ttcgaggatt tatgattgaa tcttacctag cagatggtcg tcaaaaccaa
     8701 ccagagatct ttggttgctc tattactgac ccttgcctag gttgggaaaa tacagaggcc
     8761 ttggtagaag agatttatgt taccttgaca aaataagtga aaaggatgga gttggggaat
     8821 ctcaactcct tttgatgaga atgatagttg gacacggaat tgacatcgaa gaattggctt
     8881 cgatagaaag cgcagttaca cgacatgaag gatttgctaa gcgtgtactg accgctcagg
     8941 aaatggagcg cttcaccagt ctcaaaggac gcaggcaaat agaatattta gctggtcgct
     9001 ggtcggctaa ggaggccttt tccaaggcta tgggaacggg cattagcaag ctcggttttc
     9061 aggatttgga agtcttgaac aatgaacgtg gggcgcctta ttttagtcag gcaccatttt
     9121 caggaaagat ttggctgtct atcagccaca ccgatcagtt tgtgacagcc agtgtcattt
     9181 tggaggaaaa tcatgaaagc tagtccacat agaccaacca aggctctgat tcatctggga
     9241 gctattcgac aaaatattca gcaaatgggg gctcatatcc ctcaaggaac gctcaagttg
     9301 gctgtggtca aggccaatgc ttatggtcat ggagctgttg ccgttgccaa ggcaattcaa
     9361 gatgatgttg atggcttttg cgtttccaat atcgatgaag ccattgaact cagacaagct
     9421 ggactcagca agccaatcct cattttagga gtttctgaaa tcgaagctgt tgctctagct
     9481 aaagaatatg acttcacctt gacagtggct ggactggagt ggattcaagc actcttagat
     9541 aaggaagtgg acctaactgg attgacagtc cacctcaaga ttgattcagg gatgggacgg
     9601 attggtttta gagaggcaag tgaggttgag caggctcaag atttgctcca acaacacggt
     9661 gtttgtgttg aaggaatctt tacccacttt gctactgctg atgaggaatc agatgactat
     9721 tttaatgccc agttagaacg gtttaaaact attttagcta gtatgaagga agttccagag
     9781 ctggttcatg ctagcaattc tgcaacgact ctttggcatg tagagactat tttcaatgcg
     9841 gttcgtatgg gagatgccat gtatggcctc aatccaagtg gagcggtctt ggatttgcct
     9901 tatgatttga taccggcctt gaccttggag tctgctctgg ttcatgtcaa gacagttcca
     9961 gctggagctt gcatgggcta tggagcaact tatcaagcgg atagcgagca agtcatcgcg
    10021 accgtgccaa tcgggtatgc agatggatgg acaagagaca tgcaaaattt ctctgtcttg
    10081 gtagatggcc aagcttgccc aattgtcggc agggtttcga tggaccaaat cactattcga
    10141 ttgcctaagc cttacccact cggaaccaag gtaaccttga ttggctccaa tggggataag
    10201 gaaatcactg caactcaggt agcgacctac cgcgtaacca ttaactatga ggtggtttgc
    10261 ctcctcagcg accgtattcc gagagaatat tattagaaaa gaaaggagtg gagcatgaat
    10321 ctacatcaac ccttgcatgt cttgcctggt gtgggaccaa agtcagcaga aaaatatgcc
    10381 aaactaggaa ttgaaaactt gcaagatctc ttgctctact ttcctttccg ttatgaagac
    10441 ttcaaaacca agcaggtgct ggagctggaa gacggtgaga aggcagttct ttctggtcag
    10501 gtagtgactc ctgctagtgt ccagtattat ggtttcaagc gcaatcgcct gcgttttagt
    10561 ctcaagcagg gagaggtcgt ttttgcggtg aatttcttta accagcccta tctggctgat
    10621 aaaatagagt tgggagcaac ccttgctgtc tttggaaaat gggaccgcgc taaggctagt
    10681 ctgactggga tgaaggttct ggctcaggta gaagatgacc tccagcctgt ctatcgtctg
    10741 gctcagggaa tcagtcaggc cagtctggtt aaggtcatca agacggcttt tgatcaggga
    10801 ctggacctct tgatagaaga aaatctgccc cagtctttac tagacaaata caaactcatg
    10861 tcccgttgtc aggcagtccg agctatgcat tttccaaagg atttggcaga atacaagcag
    10921 gctcttcgcc gtatcaagtt tgaggaactc ttttatttcc aaatgcagtt gcagacgctc
    10981 aagtctgaaa atagagttca gggaagtggt ctggttctga attggtctca ggaaaaagtg
    11041 acagcagtta aagtaagtct tccttttgcc ctgacccaag ctcaggaaaa gagtttgcag
    11101 gaaattttaa ctgatatgaa gtccgaccac cacatgaatc gtctcctaca aggggatgtg
    11161 gggagtggaa aaacggtagt cgctggcttg gccatgtttg cggcagtgac agcaggttat
    11221 caggctgccc taatggtacc aacagaaatc ctcgcagagc aacactttga gagtttacag
    11281 aacctttttc ccaatttgaa actggctctc ttgacaggtt ccttgaaagc tgcagaaaag
    11341 agagaagtct tggagaccat tgccaagggt gaggctgatt tgattatagg aactcacgct
    11401 ctgatacaag atggggtgga gtatgctcgt cttggtttga ttattatcga tgagcagcac
    11461 cgttttggtg tagggcaaag gcgtatttta cgggaaaaag gtgacaatcc agatgtcctc
    11521 atgatgacgg cgactcccat tccacggacg cttgccatca cagcctttgg agatatggat
    11581 gtttccatta tcgaccagat gccagcaggt cggaagccta ttgtgacgcg ctggatcaaa
    11641 catgagcaac tacctcaggt cttgacttgg ttagaggggg aaattcaaaa aggttcccaa
    11701 gcctatgtca tctctccttt gattgaagaa tcagaagctc tagatttgaa aaatgccatt
    11761 gccttatcag aggagttgac gactcatttt gcaggcaagg cagaggtggc tcttctacat
    11821 ggtaggatga agagtgacga aaaagaccag atcatgcagg atttcaagga gagaaagacg
    11881 gatattctgg tttcgacgac ggttattgag gttggggtca acgttcccaa tgcgactgtc
    11941 atgattatca tggatgccga tcgcttcggt ctcagtcaac ttcaccagct tagaggtcgt
    12001 gtcggtcggg gagacaagca gtcctacgct gttctcgttg ccaatcccaa gacggattct
    12061 gggaaagacc gcatgcgcat catgacagaa acgaccaatg gatttgtcct tgcggaggaa
    12121 gatttgaaaa tgcgtggttc tggtgagatt tttggaacca gacagtcagg acttccagag
    12181 ttccaagtgg ctgatattat cgaagatttt ccgattttag aagaagcaag aaaggttgct
    12241 agctacatta gttctataga agcttggcaa gaagatccag agtggtgcat gattgccctt
    12301 catctggaaa agaaagaaca tctggattaa gctttctcta aggaaaactt aagctagttt
    12361 ttaggtttgg ctcttatact aaagtcatca aaaagaaacg aggactctca tatgacagta
    12421 acgattaaag taaattacca aaccactttc caaaagaagg aagcaaaaaa ctagtataaa
    12481 cagaagagag agcgaaatgc tctttttcgt ttctaaaact actttcagcc catcatccta
    12541 aaagtaaaga atctaaattc actttctatt tatccttctt tcttgcattg attacataga
    12601 tatgctacag ttgtggtaac gattacaaaa taaaaggagc atgctatgaa aaatccagct
    12661 ttgctagaag aaattaagac ctatagagga agggatgagg ttccggaaga ctttgatgct
    12721 ttctgggatg gggaagtgaa aaatgtttcc acgcttccat cctaccactt ggaggaaaga
    12781 gatttccaca ttcctcaagt caagtgctat gagttaacat ttgaaggaag caaggaagga
    12841 aaggtctatg cacgcattgt tcttccaaag agtgaggaga aggttccact aatcttccat
    12901 tttcatggtt atatgggacg tggctgggac tgggccgaca tgctgggctt caccgtagct
    12961 ggttacggtg ttgtttccat ggatgtgcgg ggccagtcag gttactcaca agacggcttg
    13021 cgttctcctt taggaaatac cgtgaagggg catattatcc gtggtgctat ggaaggtcgg
    13081 gaccacctct tttataagga tgtttatctg gatatttacc agttggtcga aattgttgct
    13141 agtctgtctc aggttgatga gaagcgtctt tctagctatg gtgcctcaca aggaggggct
    13201 ctagctctag ttgcagcagc gctcaatcct cgaattcaga aaacagttgc catttatccc
    13261 ttcttgtcag acttcagacg ggtgattgag attggtaata ctagcgaggc ttacgacgaa
    13321 cttttccgtt atttcaagtt tcacgacccc ttccatgaaa cagaggagga aatcatggcg
    13381 acccttgcct atatcgatgt caaaaatctt gcccatcgta tccaaggtga ggttaagatg
    13441 attacgggct tggacgacga tgtttgctat cccattaccc agtttgcgat ttataatcgt
    13501 ctgacctgcg ataaaaccta tcgcatcatg cctgagtatg ctcacgaagc catgaatgta
    13561 tttgtcaatg accaagtcta caactggctc tgtggaagtg agattccttt taaatatcta
    13621 aaataaggag tcgactctaa gcacaaaatc ttaaaaatta caaacacgca tagtatcagg
    13681 ggattaaaaa aacttgatac tatgcgtttt atcatggaaa tatagtaaaa tgaaacaaga
    13741 ataggacaaa acgatcagga cagtcaaatc gatttctaac aatgttttag aagcaaatgt
    13801 gtactattct agtttcaatc tattatattt atagaatttt ttgttgctag atttgtcaaa
    13861 ttgcttaaaa taattttttt cagaaagcaa aagccgatac ctatcgagta gggtagttct
    13921 tgctatcgtc aggcttgtct gtaggtgtta atacttttca aaaatctctt taaaccacgt
    13981 cagcttcgcc tcgccgtata tatgttactg acttcgtcag ttctatctgc cacctcaaaa
    14041 cggtg
//
LOCUS       DAVKLX010000052        13965 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKLX010000052 DAVKLX010000000
VERSION     DAVKLX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13965
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.052"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            205..474
                     /gene="blpJ"
                     /locus_tag="VW325_001742"
     CDS             205..474
                     /gene="blpJ"
                     /locus_tag="VW325_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEU9735087.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            543..791
                     /gene="blpK"
                     /locus_tag="VW325_001743"
     CDS             543..791
                     /gene="blpK"
                     /locus_tag="VW325_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HEU9735088.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            1035..1184
                     /gene="blpO"
                     /locus_tag="VW325_001744"
     CDS             1035..1184
                     /gene="blpO"
                     /locus_tag="VW325_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9735089.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            1288..1407
                     /locus_tag="VW325_001745"
     CDS             1288..1407
                     /locus_tag="VW325_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9735090.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            1875..2246
                     /locus_tag="VW325_001746"
     CDS             1875..2246
                     /locus_tag="VW325_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735091.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            2875..3258
                     /locus_tag="VW325_001747"
     CDS             2875..3258
                     /locus_tag="VW325_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9735092.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            3310..3999
                     /locus_tag="VW325_001748"
     CDS             3310..3999
                     /locus_tag="VW325_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9735093.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            4041..4274
                     /locus_tag="VW325_001749"
     CDS             4041..4274
                     /locus_tag="VW325_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735094.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            4425..5036
                     /locus_tag="VW325_001750"
     CDS             4425..5036
                     /locus_tag="VW325_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9735095.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            5197..5991
                     /locus_tag="VW325_001751"
     CDS             5197..5991
                     /locus_tag="VW325_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9735096.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5988..6623
                     /gene="trmB"
                     /locus_tag="VW325_001752"
     CDS             5988..6623
                     /gene="trmB"
                     /locus_tag="VW325_001752"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9735097.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            6749..7228
                     /gene="rimP"
                     /locus_tag="VW325_001753"
     CDS             6749..7228
                     /gene="rimP"
                     /locus_tag="VW325_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9735098.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDGLTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            7272..8408
                     /gene="nusA"
                     /locus_tag="VW325_001754"
     CDS             7272..8408
                     /gene="nusA"
                     /locus_tag="VW325_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9735099.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            8430..8723
                     /locus_tag="VW325_001755"
     CDS             8430..8723
                     /locus_tag="VW325_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9735100.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            8716..9015
                     /locus_tag="VW325_001756"
     CDS             8716..9015
                     /locus_tag="VW325_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9735101.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            9032..11824
                     /gene="infB"
                     /locus_tag="VW325_001757"
     CDS             9032..11824
                     /gene="infB"
                     /locus_tag="VW325_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9735102.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            12075..12425
                     /gene="rbfA"
                     /locus_tag="VW325_001758"
     CDS             12075..12425
                     /gene="rbfA"
                     /locus_tag="VW325_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9735103.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            12704..13483
                     /locus_tag="VW325_001759"
     CDS             12704..13483
                     /locus_tag="VW325_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735104.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLCAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIIAGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            13485..13667
                     /locus_tag="VW325_001760"
     CDS             13485..13667
                     /locus_tag="VW325_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735105.1"
                     /translation="MNPIKAFDKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIRPEERE"
     gene            13627..13815
                     /locus_tag="VW325_001761"
     CDS             13627..13815
                     /locus_tag="VW325_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735106.1"
                     /translation="MRLGGSLGQRRENEIFFGLLAILFIKPIIGIVKFFWMIISFAVQ
                     LLFYKILFKILDWLFKLI"
BASE COUNT         4280 a         2358 c         3110 g         4217 t
ORIGIN      
        1 tgcaaggaga ataacgaaat taagatattt tagatactca agattattga aatcttagac
       61 taaaaaatag gtactgctac ctttaagaag atagtagacg tatatacttt tttaagaaaa
      121 tcaaaaagat actaaaaaaa atctatttgt ttattgaatt taagactttg gtaacaaatt
      181 gaaaataaaa aggaggtatt catcatgaat acaaaaatgt tgtcacagtt agaggttatg
      241 gatactgaaa tgcttgcgaa agttgaaggg gggtatagct ccactgattg tcaaaatgca
      301 ctgattacag gagtcactac gggaattata actggtggaa ctggagcagg tttggccact
      361 ttaggagtag ctggacttgc tggtgcattt gttggagcac acattggtgc aattgggggt
      421 ggattaacat gcttaggagg tatggttggt gataaattgg gactaagttg gtaagactta
      481 tagtaaaatt atgaggattc tattctgatt tattttagtt ttttaaaaaa ggagtatttg
      541 tcatggatac aaaaatgatg tcacaatttt ctgttatgga tactgaaatg cttgcttgcg
      601 ttgaaggtgg cggatgcaat tggggagatt ttgccaaagc aggtgttgga ggagcagctg
      661 tagttgctgc tttaggttgt gcagcgggtg gagtgaaata tgggaaaatt ctaggaccat
      721 ggggcgctgc aataggagga attggaggag cagtggtttg tggatattta gcctataccg
      781 ctacatcata aaaatgaaag ctaaatttta tgaaggctat attctttatt attctcttcg
      841 cttttcaaac ctatcttatt tatctatcca taagtatctc tgataaaaaa caaaagacta
      901 ttgaattaac atcattgaat tgttttgtca tattatttct aatctatgat aaattaattt
      961 ttctctttat tgcctatgtt tttttgataa tttttatatt aaacttgttc cgtaactgaa
     1021 aagtgacata ctatatgaat acaaaaatga tgtcacaatt ttctgttatg gataatgaaa
     1081 tgcttgcttg cgttgaaggt ggagatattg attggggaag aaaaattagt tgtgcagcag
     1141 gggttgcata tggcgcaatt gatgggtgtg caacaacggt ttgacatttc tattgggacc
     1201 atttgctata ggaataggtg taactggtgc tgcaggtgga gctatacttg gaggtgtagc
     1261 ctatgcagca acatgttggt ggtaattatg gattttaaaa gttttattat tggtttagta
     1321 gttggtatat ttggtcctta tatggatgac ttaattagaa aaatattttc aaaatcttcc
     1381 aaaaaggata cggatagtac tctctaaaaa ataaatttct cccctgtatt tgctagctta
     1441 atttacaggg gagtttcttt aatattgttt cgcctgtcaa aatgatatgt gatagcagga
     1501 tgaaaagtct acaaacaagt tggtatcaat acaatataaa gaggaaagta tgtttttcat
     1561 gcttgctttt ttaattttta caattcaaga ggttttgatg accatttatg atttgagtga
     1621 tccaaggtca aaataagtgc tatactagca gtgtaaatgt tcttgctcaa ctaagataaa
     1681 tcgcacattt aggaggaaaa cttatgaaga aaaaaatact gattattttc gttctgtatc
     1741 tgatcatgtc catctttctt tatccgctta gggagagtat ttggtataat ctattttata
     1801 ccatagccta tatgattgcg gttatgatct atttttcttt aattaaaaag aaagaaaaga
     1861 aatgaaagat tttcttgatt aaaaagaatc atattctttg tgagattgtt cctgggtcag
     1921 ttgcctttgc ttgtttctac ttatctattt ctatttcgtc agttttccaa tttcttttag
     1981 tagttattaa cttgacttct attttggtta ctgtttatct tactagggaa atgagattaa
     2041 gagagtttga agatgatgat ttggttagtc ctagaaccaa tcaactcatg tttatcggct
     2101 tgacaggctt tatgtctatt atttgtttgt atagaggtat cacagcagga gaatcttatc
     2161 aacaactaat cgcttatatt ggtgctattc tttgcttgat catcatgctt ctactcattt
     2221 ggggtttgaa gtattataaa aagtaggggc taatgaagtt tttgcaattt aggaggtttc
     2281 gatgacaatt caagatttgg atgaaaaatt ttaaaaaaca atgatatact aaacttgtca
     2341 aagttgcaac gggtgctgct acattatgtt aaaaatcgct cttgattgat aagttggatt
     2401 tgttaggaaa ggagtaaata attatggata agaaaaagat agtttcgact ataatatgta
     2461 tagtatttct tgtagtgtca gtagataatt tttttagaga cttaaccccg ttactgttca
     2521 ttttaaatat tattggcctg tcttgctttt cagtgctaac atacattaac ataaaagaga
     2581 tactcttaaa tataagtaaa tgatgatttg gttagtccta gaaccaatca acttatgttt
     2641 atcggcttgg cagattttat gtttgtcatt tgtttgtata gaggtatttc agaaacagaa
     2701 ttctatcaac aactaatcgc ttatataggt gtattctctg cttgtttatc acgcttctgc
     2761 tcatgtgggg cttgaagtat tataaaaagt agctttctat ccttttctga caggatattt
     2821 gagtagaagg ctctattaaa ttgttagtga aataaatgaa tggaggtatt taatatgaaa
     2881 tacagattat tttttgttat tttcttgagt agtgtgttgg atattctttt agggacattt
     2941 ttacaaatct ctatcgtatc tattgggtgg cttgttcttt acagtggttt gtttgaagca
     3001 ggagttttcc ttcttgctaa taaaggggtg gcggtaaaaa tcaaggaagt agatattcga
     3061 aatcgcttta aatttatttt tggaaaaacc ttatggtttc aaattctttt gctcatcttt
     3121 ttgataatca aactttatct tggtttggat gcgaggttga ttttattcta tggacatatt
     3181 ttcattgtct ttaatgcctt aatgtatcta ttatctagta gtcaggttag ccttaaaaaa
     3241 aacaaactgt cttcttaatc ttacagcagt agagcctgac aaggtgaggt catagtaggg
     3301 aggatttcta tgaaaaagta tcaacttcta ttcaaaataa gtgcagtttt ctcttactta
     3361 tttttcgtat ttggtctttc tcagatgacg cttattattc aaaattattg gcaattttct
     3421 tcccagattg gcaatttcgt ctggattcaa aatttcttga gtttgctatt tagcggagtc
     3481 atgatttgga ttctggttaa gacaggtcat ggttatctct ttcacattcc aagaaaaaaa
     3541 tggctttggt attcgatttt gacagtatta gtggtagtgc tccagatctc ttttaacgtt
     3601 cagacagcta aacatgttca gtcaactgct gaaggttggg ctgtattgat cggttatagt
     3661 gggaccaact ttgctgagct aggtatctat ataactttgt tctttctgac tccacttatg
     3721 gaagagctaa tctatagagg attactgcaa cacgcctttt ttaagcattc gagatttggc
     3781 cttgatttgc ttcttccgtc aattttattt gctcttcctc atttttcaag cctgcctagt
     3841 ttgttagata tcttcatctt tgcaacatct ggcatcatct ttgctagttt gacccgctat
     3901 accaagagca tttatccttc ctatgcggtg catgtgatca ataatatttt cgcaacatta
     3961 ccatttttgc tgactttttt acatagggtc ttggggtaaa aaaacaaaag gcttgctttt
     4021 cagccataga ggagattatc atgtataaac acttattttt cctagattcc aaaactttag
     4081 atcggttgac accctatatt ctagtcttgg cttctgacac cattgctttt aatgtttttg
     4141 tgctaacctt tgtatctgcg gtggttttta atttcctaaa ttccatgcta gctttaatgg
     4201 ctatattcat aggggctggc tatgtggtcg gattttggtt actaatactc aatgaaaatc
     4261 aaagagcaaa ctaggaaact agccgcaggc tgtacttgag tacggcaagg cgacgttgac
     4321 gtggtttgaa tttgattttc gaagagtata aaatggtttg ttttggaaag gctagaacta
     4381 aagaatgacg cgtaggaagt ctgtttggtg aggaggatag ttttatggag ttttttgata
     4441 aatttcatgc cttgtgtttt ggatttttag tactaataat tgtcattaca gttccttata
     4501 cgattaacca tgggggtttt tttcaaaatg aatctgcatt gattcttgta agtcttcttg
     4561 taacctcgct gagtgttgct tatgctagaa agtttgaaat gatttctttt gggatgttaa
     4621 gcaagaaaca acttttgctt ttcattgcaa tctttcttct aagtgtactt gagacgctgg
     4681 tttatattca tttcttcgct gtttcttctg gctcaggggt ccaacacttg gcggaagtca
     4741 gcagaggaat ttccctgtct ttgattttga ctacctcagt ttttggcccc atccaggagg
     4801 aactcatttt cagaggactt cttcaaggtg cggtttttga caattcttgg ttagggcttg
     4861 tgctaacttc ctctctcttt tctttcatgc atggaccttc taatgtccct tcgtttattt
     4921 tttatctact tgggggtttg ttgctgggct ttgcttataa aaagagtcaa aacctatggg
     4981 tttctactct agtccacatg ttttacaaca gttggccact cctatattat ttataaaaat
     5041 tatgaaaagg taagcagaag atggtgctta cctttttcaa gctataatga tacccaagcc
     5101 aatccagagg cttttctttg agaatcgggt tagagtggtt gacgaatagg ccaaaaacta
     5161 gtagaatagt aaggaaactt tatacggagg aaagaaatgg atttgggtga taatgagcta
     5221 acactgactc ccatacctgg gaaaagtggc aaggcttata tgggtagcta tcctgacggg
     5281 aagcgcatct ttgtaaaaat gaacacctct ccaatcctac ctggtctagc tagagaacaa
     5341 attgctccac aattattatg gagtcgtcgt ttggcagatg ggcgtgatat gtgtgctcaa
     5401 gaatggttga caggcaagat attgaccccc tatgatatga atcgtaagca aatcgtcaat
     5461 attttaaccc gtcttcatcg ctcacgtccg ttgatgacac aattgagtcg tttgggctat
     5521 gccatggaaa cacctgtaga tttactccag tcttggcagg aaacggctcc agatgctttg
     5581 cgtaaaaatc attttatcag tgaagtgatg gctgatttac gtcagactat tccaggattt
     5641 agagaggacc atgcgaccat tgtccatgga gatgtacgac atagtaattg gattgagaca
     5701 gatagtggct tgatttattt agtagattgg gattcggttc gcttgaccga tcgcatgttt
     5761 gatgtggccc atatgctctg ccattatatt tcagaacatc agtggaagga atggttgacc
     5821 tactacggtt acaagtacaa tcaaacggta ttaagtaaat tgtattggta tggtcaattg
     5881 tcttatttga gtcagatttc caagtattat atgaaccaag atttagaaaa tgtcaatcgg
     5941 gagattcatg gtttgcgtca tttccgagac aagtatggaa agagaagatg agagttagaa
     6001 atcgtaaagg ggcaacagaa ttactagagg caaatcccca gtatgtggtc ctcaatccct
     6061 tggaagccaa ggcaaaatgg cgggacttgt ttggcaatga taatcccatt catgtggaag
     6121 ttggaagtgg aaagggtgcc tttgtttcag gtatggccaa gcaaaaccct gacatcaact
     6181 atatcgggat tgatattcaa aagtctgttt tgagctacgc tttggacaag gtgcttgaag
     6241 ttggagtgcc taacatcaag ctcttgtggg tagatggttc tgacttaact gactattttg
     6301 aagacggtga gattgatcgc ttgtatctga acttttcaga tccatggccg aaaaaacgcc
     6361 atgaaaagcg tcgtttgacc tacaagacct tcttggatac cttcaaacgt atcttgcctg
     6421 aaaatggaga aattcatttc aagacggata accgtggctt gtttgagtac agtttagtga
     6481 gcttttctca atatggcatg aaactcaatg gtgtctggtt agatttgcat gccagtgatt
     6541 ttgaaggcaa tgtcatgaca gaatacgagc aaaaattctc aaacaagggg caagttatct
     6601 accgagttga ggcagaattt taagagatag cctaaaatta ggctgtacaa gtgcttttgc
     6661 tttacataag ttggcaaacg tgctatactg atagtaagaa tatgaaaagt gaggcgggga
     6721 aatatcttcg cctcttgctt atgaggaggt ggacgcaatc gcaacaatcg tagaattagt
     6781 cagagaagtt gtagaacctg tcatagaagc tccttttgaa ctcgtggata tcgagtatgg
     6841 aaagattggc agtgacatga ttctcagtat ttttgtagat aaacccgaag gaattacctt
     6901 gaacgacacg gcagacttga cagaaattat cagtcctgtc ctagacacca tcaagccaga
     6961 tcccttccca gaacaatatt tcctagaaat taccagtcca ggtttggaac gccctttgaa
     7021 aaccaaggat gccgtcgctg gagcggttgg aaaatacatc catgtcgggc tctaccaagc
     7081 catcgataag caaaaggtct ttgaaggaac cttgttggcc ttcgaagagg acgggttgac
     7141 tatggaatat atggacaaga cgcgtaagaa aaccgtccaa attccataca gtttagtatc
     7201 aaaagcacgt ttagcagtta aattatagaa aaagaaagga tagcttttga ggattcaaaa
     7261 gtgaagaaaa catgagtaaa gaaatgctag aggccttccg cattttggaa gaagacaagg
     7321 gaatcaaaaa agaagatatc atcgacgcag tagtagagtc gcttcgttcc gcttatcgca
     7381 gacgctatgg tcagtcagac agcgtagcta ttgacttcaa cgaaaaaaca ggtgacttta
     7441 cagtttatac tgtccgtgaa gttgttgatg aagtatttga tagccgtttg gaaatcagct
     7501 tgaaagatgc tcttgccatt aattcagctt atgaacttgg agacaaaatc aagtttgaag
     7561 aagcaccagc tgagtttggt cgtgtagcag cccagtctgc caaacaaacc atcatggaaa
     7621 aaatgcgcaa gcaaacacgt gccatcactt acaatactta caaagaacat gagcaagaaa
     7681 tcatgtctgg tacagtagaa cgctttgaca accgctttat ctatgtcaac cttggtagca
     7741 tcgaagccca attgtcaaaa caagaccaaa ttcctggaga agtttttgct tctcatgatc
     7801 gtatcgaagt ttatgtttac aaggttgaag acaaccctcg tggtgtgaac gtctttgtta
     7861 gccgtagtca tccagaaatg atcaaacgtt taatggagca agaaattcca gaagtttatg
     7921 atggaactgt tgaaatcatg agcgtggctc gtgaagcagg tgaccgtacg aaggttgctg
     7981 ttcgtagcca caatccaaac gtggatgcta tcggtacaat cgttggacgt ggtggtgcta
     8041 atatcaagaa gattactagc aaattccacc cagctcgtta cgatgctaaa aatgaccgca
     8101 tggtaccaat cgaagaaaat atcgatgtta tcgagtgggt agcagatcca gctgaattta
     8161 tctacaatgc catcgctcct gctgaggttg accaagttat ctttgatgaa aacgacagca
     8221 aacgtgcctt ggtggttgtt ccagataaca agctttctct tgccattggt cgtcgtggac
     8281 aaaacgtgcg cttggcggct cacttgactg gttaccgtat cgatatcaag tctgctagcg
     8341 aatttgaagc catggaagac gctgcttcag tagagttgga agtagaaaac gatactgtag
     8401 aagaataaaa gctgctagag gagggaaaga tgaaaacaag aaaaatccct ttgcgcaagt
     8461 ctgttgtgtc taacgaagtg attgataagc gtgatttgct ccgcattgtc aagaacaagg
     8521 aaggacaagt ctttattgat cctacgggca aggccaatgg ccgcggcgct tatatcaaac
     8581 tagacaatgc agaagcccta gaggcgaaaa agaagaaggt ctttaaccgc agctttagca
     8641 tggaagtgga agaaagcttt tatgacgagt tgatcgctta tgtggatcac aaagtgaaaa
     8701 gaagagagtt gggacttgaa taagcaaaag ataagtaatc tcttggggct tgctcagcga
     8761 gcagggcgca tcatatcggg tgaagaattg gtggtcaagg ccattcaaga cggcaaggcc
     8821 aagttggtct ttctagctca tgatgctgga cccaatctga ccaagaagat tcaagataaa
     8881 agtcattatt atcaagtaga aattgtaacc gtgttttcaa cactggaatt aagcatagca
     8941 gtcgggaaat cgagaaaggt tttggctgta acagatgctg gatttacaaa gaaaatgagg
     9001 tctcttatgg aatagaagag gaggacatga tttgtctaag aaaagattgt acgaaatcgc
     9061 aaaagaactt ggaaaagaaa gtaaagaagt tgtagcgcgt gcaaaagagt tgggcttgga
     9121 tgtgaaaagc cactcatcaa gtgtggaaga agctgtcgct gcaaaaattg ctgccagctt
     9181 taagcctgca gctgctccga aagtagaagc aaaacctgca gcaccaaaag taagtgcaga
     9241 aaagaaagcc gaaaaatctg agccagctaa accagctgta gctaaggaag aggcaaaacc
     9301 ggctgagcca gttgctccga aaacagaaaa agtagcagtg aaaccgcaaa gtcgtaattt
     9361 caaggctgag cgtgaagccc gtgctaaaga gcaggcagaa cgacgcaagc aaaataaggg
     9421 caataaccgt gaccaacaac aaaacggaaa ccgtcagaaa aacgacggcc gtaatggtgg
     9481 aaaacaaggt caaagcaacc gcgacaatcg tcgctttaat gaccaagcta agaagcagca
     9541 aggtcagcaa aaacgtagaa atgagcgccg tcagcaagag gataaacgtt caaatcaagc
     9601 ggctccacgt attgacttta aagcccgtgc agcagcccta aaagcagagc aaaatgcaga
     9661 gtacgctcgt tcaagtgagg aacgcttcaa gcagtatcag gctgctaaag aagccttggc
     9721 tcaagctaac aaacgcaagg aaccagagga aatctttgaa gaagcggcta agttagctga
     9781 acaagcacag caagttcaag cagtggttga agtcgtccct gagaaaaaag aacctgcagt
     9841 ggatacacgt cgtaaaaaac aagctcgacc agacaaaaat cgtgacgatt atgatcatga
     9901 agaagatggt cctagaaaac aacaaaagaa tcgaagtagt caaaatcaag tgagaaatca
     9961 aaagaatagt aactggaata acaacaaaaa gaacaaaaaa ggcaataaca agaacaaccg
    10021 taatcagact ccaaaacctg ttacggagcg taaattccat gaattgccaa cagaatttga
    10081 atatacagat ggtatgaccg ttgcggaaat cgcaaaacgt atcaaacgtg aaccagctga
    10141 aattgttaag aaacttttca tgatgggtgt catggccaca caaaaccaat ccttggatgg
    10201 ggaaacaatt gaactcctca tggtggatta cggtatcgaa gccaaacaaa aggttgaagt
    10261 ggataatgct gacatcgaac gtttctttgt cgaagatggt tatctcaatg aagatgaatt
    10321 ggttgagcgt ccaccagttg ttactatcat gggacacgtt gaccacggta aaacaaccct
    10381 tttggatact cttcgtaact cacgtgttgc gacaggtgaa gcaggtggta ttactcagca
    10441 tatcggtgcc taccaaatcg tggaaaatgg taagaagatt accttccttg atacaccagg
    10501 acacgcggcc tttacatcaa tgcgtgcgcg tggtgcttct gttaccgata ttacgatctt
    10561 ggtcgtagcg gcagatgacg gggttatgcc tcagactatt gaagccatca accactcaaa
    10621 agcagctaac gttccaatca tcgtagccat taacaagatt gataaaccag gtgctaaccc
    10681 agaacgcgtt atcggtgaat tggcagagca tggtgtgatg tcaaccgctt ggggtggaga
    10741 ttctgaattt gttgaaatct cagctaaatt caaccaaaat atcgaagaat tgttggaaac
    10801 agtccttctt gtagctgaaa tccaagaact caaggcagac ccaacagtgc gtgcaatcgg
    10861 tacggttatc gaagcgcgct tggataaagg aaaaggtgcg gtcgcaaccc ttcttgtaca
    10921 acaaggtacc ttgaatgttc aagatccaat cgttgtcgga aataccttcg gtcgtgtccg
    10981 tgctatgacc aacgaccttg gtcgtcgtgt taaagttgct ggaccatcaa caccagtctc
    11041 tatcacaggt ttgaacgaag caccgatggc gggtgaccac tttgccgttt acgaggatga
    11101 aaaatctgcg cgtgcagcag gtgaagagcg tgccaaacgt gccctcatga aacaacgtca
    11161 agctacccaa cgtgttagcc ttgaaaacct ctttgatacc cttaaagctg gggaactcaa
    11221 atctgttaat gttatcatca aggctgatgt acaaggttct gttgaagccc tttctgcctc
    11281 acttcaaaag attgacgtgg aaggtgtcaa agtgactatc gtccactcag cggtcggtgc
    11341 tatcaacgaa tcagacgtga cccttgccga agcttcaaat gcctttatcg ttggtttcaa
    11401 cgtacgccct acaccacaag ctcgtcaaca agcagaagct gacgatgtgg aaatccgtct
    11461 tcacagcatt atctacaagg ttatcgaaga gatggaagaa gctatgaaag ggatgcttga
    11521 tccagaattt gaagaaaaag ttattggtga agcggttatc cgtgaaacct tcaaggtgtc
    11581 taaagtggga actatcggtg gatttatggt tatcaacggt aaggttgccc gtgactctaa
    11641 agtccgtgtt atccgtgatg gtgtcgttat ctatgatggc gaactcgcaa gcttgaaaca
    11701 ctacaaagac gacgtgaaag aagtgacaaa cggtcgtgaa ggtggattga tgatcgacgg
    11761 ctacaatgat attaagatgg atgatgtgat tgaggcgtat gtcatggaag aaatcaagag
    11821 ataagatttt ttgctccttt cttaggtggt gagggacgca agcaaaccga tggtttcatt
    11881 gcttattttt gagcctaggg tctcaaaaat cccctgtgat gggactgata aatcagttcc
    11941 atcactttca ccacggcgaa agaagcagat gacttcaaat tgaacttcgt ttcaatttaa
    12001 actgaaaatc aagaagttta aaatagctag gtctgctggc ctagcttttg gttcaaagta
    12061 gagaaaggaa tatcatggca aatcatttcc gtacagatcg tgtgggcatg gaaatcaagc
    12121 gtgaagtcaa tgagattttg caaaagaaag tccgtgatcc acgtgtccaa ggtgtgacca
    12181 tcacagatgt tcagatgctg ggtgacttgt ctgttgccaa ggtttattac accattttga
    12241 gtaaccttgc ttcggataac caaaaagccc aaatcgggct tgaaaaagca actggtacca
    12301 tcaaacgtga acttggtcgc aatttgaaat tgtacaaaat cccagatttg accttcgtca
    12361 aagacgagtc catcgagtat ggaaacaaga ttgacgagat gctacgcaat ctggataaga
    12421 actaaagaag aggggttgcc cctctttttt ggtggaggaa aataggttga atttgaaatg
    12481 gaaaaatatt cttttaaaat atattgaaac tagaatagta cgcctctact tctaaaacat
    12541 tgttagaaat cgatttgact gtcctgatcg atttgtcctg ttcttgtttc attttactat
    12601 aaaaaagggg ttctgtattt tttaatgtta tctaattaga aaatgctttt tttgtaggaa
    12661 atataatatg ataaggtgta aaaaagaaat aaggagtttg tatatggctg aacaagactt
    12721 agctatgcaa gtattgcaac aagtggtgaa actacctgtt gttaaggttg atcgttcgaa
    12781 atttttagtg gataagtttt ccaaagaatt ggatccaaaa gatattccta ccttattgga
    12841 acaaggtaca acgactcttc tatctcaaga aatattagat cgtgtagcta atgcttgtat
    12901 tcgggacaat gtattattag cgagtgggac ttctgttttg gcaggattag ctggagggct
    12961 tgctatggca attaccattc cagctgatgt ggctcaattt tatgctttct ctctgaaatt
    13021 ggctcaagaa ttaggttata tttatggtta tgaggatctt tgggcttcac gcgaggagtt
    13081 gagtgaagat gctcaaaata ccctcttgct ttatctaggc gtaatgttag gggtgaatgg
    13141 aaccgctgct ttgctatgtg ctggtggtat aacaattgcc aaacaggtaa tgaaaacagt
    13201 gcctaataaa gctttaacaa agacgctttg gtaccctatt ttgaaaaaag tcttaaaaat
    13261 atttggtgtg aatcttacca agggagggct ggccaaagga atagggaaat ttattcctat
    13321 cttgggtggt atcattgcag gtggtttaac ctttgcaact atgaaaccaa tgggggaaag
    13381 cttgcagaaa gaattatcca agctagtcaa ctatagtgaa gttcaatatc aagaagatgt
    13441 tgaaacaatc cgaaaagagg ctgaaatcat taaaggagag taatatgaat cctatcaaag
    13501 cttttgataa aatttatggt aattactttt tgaccgtgca aggtgtaaaa gtgatgaaaa
    13561 cgataaagaa agctgaccat gtcgttgttg gtctgggaaa actttttatt gcagataagc
    13621 taatggatac ggctcggtgg ctcattaggc cagaggagag agaatgaaat tttttttggt
    13681 cttcttgcta ttctttttat caaaccgatt attgggattg tgaaattctt ttggatgatc
    13741 atttcctttg cagttcaatt gctgttttac aagatattat ttaagatatt ggattggctc
    13801 tttaaactta tctagatggt aatccaagtc gcagagagct agcaggaact ccactgctag
    13861 ttttttaatc tctctccata tggtataata taagcagtaa aatcatttta tactcttcga
    13921 aaatctcttc aaaccacgtc agcttcacct tgcagtatat atgtt
//
LOCUS       DAVKLX010000053        13734 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKLX010000053 DAVKLX010000000
VERSION     DAVKLX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13734
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.053"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(779..1648)
                     /locus_tag="VW325_001762"
     CDS             complement(779..1648)
                     /locus_tag="VW325_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9735107.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
     gene            complement(1872..2549)
                     /locus_tag="VW325_001763"
     CDS             complement(1872..2549)
                     /locus_tag="VW325_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9735108.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKI
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPIPEP
                     TPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGFF
                     KKKEDEK"
     gene            complement(2507..3613)
                     /locus_tag="VW325_001764"
     CDS             complement(2507..3613)
                     /locus_tag="VW325_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9735109.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(3724..3978)
                     /locus_tag="VW325_001765"
     CDS             complement(3724..3978)
                     /locus_tag="VW325_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735110.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(4318..4764)
                     /locus_tag="VW325_001766"
     CDS             complement(4318..4764)
                     /locus_tag="VW325_001766"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9735111.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDLLREVPTIFECLECFFKTR"
     gene            complement(4837..5162)
                     /locus_tag="VW325_001767"
                     /pseudo
     CDS             complement(4837..5162)
                     /locus_tag="VW325_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284991.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5396..7114
                     /locus_tag="VW325_001768"
     CDS             5396..7114
                     /locus_tag="VW325_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9735112.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            7225..7572
                     /locus_tag="VW325_001769"
     CDS             7225..7572
                     /locus_tag="VW325_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9735113.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     ADTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(7850..8686)
                     /locus_tag="VW325_001770"
     CDS             complement(7850..8686)
                     /locus_tag="VW325_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9735114.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(8699..9328)
                     /locus_tag="VW325_001771"
     CDS             complement(8699..9328)
                     /locus_tag="VW325_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9735115.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASELLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(9338..9979)
                     /locus_tag="VW325_001772"
     CDS             complement(9338..9979)
                     /locus_tag="VW325_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9735116.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(10125..11354)
                     /locus_tag="VW325_001773"
     CDS             complement(10125..11354)
                     /locus_tag="VW325_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9735117.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSNLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(11344..12510)
                     /locus_tag="VW325_001774"
     CDS             complement(11344..12510)
                     /locus_tag="VW325_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9735118.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            12654..13667
                     /locus_tag="VW325_001775"
     CDS             12654..13667
                     /locus_tag="VW325_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9735119.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEKGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
BASE COUNT         4148 a         2939 c         2446 g         4201 t
ORIGIN      
        1 taaaaattta actaattaat agaaagtact aaatagactt atccttcaag gagttttcag
       61 cattatccat ccactcactt gctgaaaatg taaacagtct ctggtttata tagtaaaatg
      121 aaacaagaat agagcaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa
      181 gtagatgtgt actattctag tttcaatcta ctatacaacc tcaaaactgt gttttgaaca
      241 acctgcggct agcttcttag tttgcccttt gattttcatt gagtataaaa ttgcagataa
      301 aacgataaca atcataggac tcagcagagt caatgtaggt cgaagaatag cccccctaat
      361 cgcttatttt atagcaaaat gaaatagaaa ctgaacattt tgttgaggaa ggtaaatcaa
      421 ttcctcacaa tgtttgagaa agaagggagg actattccaa cttcaaccca ctataaagca
      481 gacttacaag atagggaacc catctccgat aatatatagt agaatgaaaa ctgcacaatt
      541 tgattggaga attcaaacca atttctcaca atgttttaga aacaagggtc tattatttca
      601 actgcaacct actatagtcc attgaagctg gaatagccca ctgagacttc taaaccactg
      661 ctggagagga gtttgtttag agtttctttc atttccctaa aaaatcaaaa agagagaacc
      721 agtctgatcc tctcttactt atagatggtg ctcactcaaa tgttaaagga gctatttctt
      781 agagataaag gaatttgaat acagctactg ccgcaattgc tgctacgata ggtgctacta
      841 ctggtaccca agaataccac cattttgaat cgcctttatg ctcaccaaga actgattttg
      901 gaaggaaagc atgaaggaga cgtggtccca agtcacgggc tgggttcaag gcaggtcctg
      961 taggtcctcc aagtgatgtt accaaagcca taacgaggaa tccaagtgcc aagtgagcca
     1021 cagaaagtcc tgaagcagtg tgtggagcca cctgtgcttt aatagccaag tcagaaaaat
     1081 caactgtttg tcctgcttct attgcctttt gtttcatgaa ttgaagcact tcagcaccaa
     1141 agaagttttt agtcaaacca agagctgcaa agaacaaaac aaatgaacca acaaactcat
     1201 taatcaaacc attgacagtt gctgcatagc gactttcttt tgtaccatgg tcaatacttg
     1261 aaatagttga gaaagttccc aagatgttat ttgggttttc agttttcaag tagaatggac
     1321 ggtatgttgc cacaactaag gcttggccaa agatagctcc caagacttgc gcgataatat
     1381 aaggtaccac ttgtgcccaa gggaaaagac cgctaactgc aagccctaga gtgaaagcag
     1441 ggttgatgtg attcccagat acgttaccaa acatcaaggc tgggatcata acccccatac
     1501 cataaccaac agcgatgacg atccagccac tttggtgacc tttcgtacct ttaagttcaa
     1561 cgttggcaac tgcaccattc ccaagaatga tcaaaatggc agttcccaaa aattcagtgg
     1621 catacttcag tgcccatgtg aaatccattt gctagattct ccttaaaatt ttttagatat
     1681 tccttatttt atcaattttt accctattta gcaactgatt tctggaaata tttttaaaaa
     1741 aataggattc atagtttccc agttcaacgg acttgtaagc gtttttattc ttcacgaaag
     1801 acctcgttct caaaaccagg aaaaaagccc acatctgtga gcttttcctg gttcctagat
     1861 tctaaaatct attatttttc atcttctttt ttcttgaaga atcctaatcc tagaccagct
     1921 aacaaggtca tgataccagc actagccaag gtagcattag cttctgtacc tgtgtttggc
     1981 aattcctctc tcttacctgt ctcataagtc ggaacttctg ggtctggatt cactggagtt
     2041 tcagtttttg gagtacctgg ttctggagtt ggctctggta ttggagtctc ttgatttggt
     2101 agttctgggt ttggtgtcgg aacacttgga tgctctggat ttggttgtgg gattggtttt
     2161 tctggagtcg gttcacctgg agtacctggt tcctgaggac ttggaatttc tggtttttct
     2221 ggagttggtt tatctggtgt tgataaacgg tcatacctta ccgttatttc tttatcacta
     2281 gagtctgacg taacttcttg tgattcaact gttggaatat ctggatcttt gtacttgtca
     2341 atcttaccag atacaacctc gtcccagttt cctgttgtcc attcaccgta ggttacaact
     2401 cccgtgacct tgttctcaat ttttgtacgg cttaaggtta caggttgaac aacatcttct
     2461 tttacatttt ggttcgtaac tttatcaacg taatgaatga tacgcgttat agtcttcgtc
     2521 tcagtagagg ttgctgtttt gggaaccact gtttcctcaa cattctcacg gtagtaatag
     2581 tcaactgttg caccgtcttc tggtacgcat ttgcaggagt tgctaccaag gtgtatgttg
     2641 ttttccttgt gataactcgg tcttctttgt cctcagttgt tgttttccct tcaatagttt
     2701 ttgattctgt ggtatactca gaacctatcg ctaaatcagc ttttataaca gactctgcca
     2761 acttctcttg gctaccttct ttatagtaat tggctgttac tgtagcagtg gttggcgctt
     2821 cgctttctct ataaactaag gtcactgttc taccttcgct tacaatattc ccagttaaac
     2881 ttgcagaatt tgtatctgct tctttaaaag tataatattt tccgtcagta gtagtcatgc
     2941 tactgagttt tttatctgtg acattgtagc tggtaccaat cagttgtttt ttattggtaa
     3001 tgtaggttcc gtcactttct ttttctccaa ttccagtatc atttttcaat gatagcaaac
     3061 gcccttgttc atcaacatag cgaactttca cattttctga gattagttct gccaattctg
     3121 aggttttttt ctttttcttg atttcttcgg ttattttccc tttctcttct tcgggaatat
     3181 ttagttttgg aatgattttt tcaacaacgg ttcgtgatgg ttccacagta tcttggatga
     3241 ctgaaaagtc agctagaatt gggagattat actgaacacg gtgactttga gtgtttactc
     3301 ctactctttc attattctct gaaaatactt gtacggtata agaaacaaca tcttttccta
     3361 atagaacatc cccagtagag aaatagccgc cttttcctag tttgctatct ccagagccca
     3421 cttctttcct aatcttatca gatagttttt taccagtcag tacattcgtt cgcacaatcc
     3481 ctttgtctac ccctacaaag tgggagaact ttttgaactc ttcagaacca gatctagccc
     3541 aaccattatt aagggcattt gcttttgtat ttgtattctc tctcaaaagt ttggcgatta
     3601 gaattatatt catcggcact tagagttgct gctatatctg actcttgaat accaacttcc
     3661 ttactaccat ttctagcggc agtatatgtg tatttatctg tttctacttc tagctgatta
     3721 tccttaacat gataggtaat tttctcctgg tataagagtc ttcagtagtt tatatactct
     3781 gtcatcatct ttagctttag aagcagctga gtttagccaa gttggggtat taggagtatc
     3841 tttatcagcc tgtttcaaga cattattcaa gtcatttgct agcctattgg ttatcctcac
     3901 tgtgaaagtt ttcttccagt ctgaaccact tttattagac ttagatatgc tcatgaaatc
     3961 ctggtcaaca ttcaacacaa tatcgccttg ttttttagca tcttccgttg cttctgcttc
     4021 aaattgatca tggagttttt gttgctctgg tgtatagttt ttttatttac acgatttaat
     4081 gtactatttt ttattttagt attaagaact ccattaatac gatttttaaa ctctgcttca
     4141 aaaaatccct taatttgttg gtgcgcaagt tcaacattca aacctttctt atacaagtct
     4201 tcattctgaa cgatttcggc tacacggtga atggtttgat tgtataccgt cttctcttaa
     4261 ccagcagtat ccttagtttt aatgactact cgataggttc ctttagaacc gttatagtca
     4321 tctagtttta aaaaagcatt ccaaacattc aaaaatagtt ggaacttccc ttaataaatc
     4381 cgtcactttc tttttcaaga tagcccaagc ttgctcaata ggattcaaat ccggtgaata
     4441 aggtggtaga ggtaagaaaa agtgtttatc ttggatgcag agttcatcca aaatgttctt
     4501 gggatgaaaa ccagcattgc ccatgacaat aacatgaggt gtcttcaaag caggtaggag
     4561 ttgcgttttg aaccactcca caaagaaatc gcttgtcatg cttttcttgt aaatcatggg
     4621 agctataaac tctccgtcta cttgtcctgc aacaattgaa gttcgctcaa aacgacgtcc
     4681 gctaatcttt tcatagactt tctcccctct aggagcccct gcataaggac gatagagata
     4741 gcggtcgatt cctgtttcat caatataaac aggtgctagg tgctttaaac tattaaaatt
     4801 cttaagaaat aaggctactt ttctgggtct tgttccttaa agctcctcat ctttttttaa
     4861 agtgacctta atctgcttta aagctgccca tactgaagga acagcacaat caaaatgtgc
     4921 cgcaatttcc cgtaaaaaag catctggatg agcctctaca aaggctttca attcctctaa
     4981 aggaatcttt cggtttttga cgactcgctt tttcctctct atgtgttctt gttcacgtct
     5041 ttcttttccc acgtgaagag agttctgacg ccaacaccaa aaaacttggc tgcctcgaca
     5101 tggctgtgcc cctctttgat gtaatctaat gctcgctgtt taaaatcagt actatatgcc
     5161 atagctttat tataatatgc ttgtttaaaa ctaagtaact atatcaatgt tttgttatga
     5221 gaagacttac tttttttact gaaattgaat ttttcctagc ctcaacatca tataacccca
     5281 aaattcaatc tgacctggtt tgaagagatt ttcgaagcag ataaggtttt actaaaacta
     5341 aaaaaaatga tataattaaa ctgattaaat gttttaaaaa taaaggagaa tttgaatgtc
     5401 ttaccaagaa aattaccaga aatgggttga ttttgtggag cttcctgact accttcgtca
     5461 agatttggaa aatatggacg aaaaaactaa ggaagatgcc ttctatacaa atcttgaatt
     5521 tggtactgca ggtatgcgtg gcttggttgg tgctggtaca aaccgtatca acatctacgt
     5581 tgttcgccaa gctactgaag gattggctcg tttgattgag tcaaaaggtg gaaacgagaa
     5641 agaacgcggt gtagcaattg cctacgatag ccgtcacttc tcacctgagt ttgcctttga
     5701 atctgcggca gttcttgcta aacacggcat caaatcttac gtatttgaaa gccttcgtcc
     5761 aactccagaa ctatcatttg cagttcgtca tctcaactgt ttcgcaggta tcatggtcac
     5821 agccagccac aaccctgctc catttaacgg ttacaaggtt tacggtgaag acggtggaca
     5881 aatgcctcca cacgatgcgg acgctttgac tacttatatc cgtgcaatcg aaaacccatt
     5941 tgcagttgaa gttgctgatg tggaaactga aaaagcttct ggcttgattg aagttatcgg
     6001 cgaagctgtt gacatagaat accttaaaga ggttaaggac ataaacatca acccagcctt
     6061 gattgaagaa tttggtaaag acatgaagat tgtctacaca ccacttcatg gtactggtga
     6121 aatgttggct cgtcgtgctc ttgcccaagc aggatttgac tctgttcaag ttgttgaagc
     6181 gcaagcaact gctgacccag acttttcaac tgtaacatct ccaaacccag aaagccaagc
     6241 agcctttgcc cttgctgaag aacttggtcg tcaagttggt gcagatgttc ttgtcgcaac
     6301 tgacccagac gctgaccgtg ttggtgttga agttcttcaa aaagatggta gctacctcaa
     6361 cctttcaggt aaccaaatcg gtgctatcat ggctaaatac atcttggaag ctcacaaaaa
     6421 cgctggaact cttcctgaaa atgccgctct ctgcaaatct atcgtttcaa ctgacttggt
     6481 aacgaagatt gctgaaagct acggcgcaac tatgttcaac gtcttgacag gtttcaaatt
     6541 tatcgctgaa aaaatccaag aattcgaaga aaaacacaac cacacttaca tgatgggatt
     6601 tgaagaaagc ttcggttact tgattaaacc atttgtacgt gataaagatg ccatccaagc
     6661 cgttcttgtc gttgctgaac ttgctgccta ctaccgttct cgtggtttga cacttgctga
     6721 cggtatcgaa gaaatctata aagaatatgg ctactacgca gaaaaaacaa tctctgttac
     6781 tctttcaggt gtcgatggtg ctgaacaaat caaagcgatt atggctaaat tccgcaacaa
     6841 tgctccaaca gaatggaacg caacagctat cactgtcgta gaagacttca aggcacaaac
     6901 tgctactgtt gctgacggta ctgttacaaa cttgacaact cctccaagtg atgtgttgaa
     6961 atacacactt gctgacggtt catggattgc cgttcgccct tcaggtacag aaccaaaaat
     7021 caagttctac attgcagttg taggggaaac caacgaagaa tcacaagcta agattgctaa
     7081 catcgaagca gaaatcaatg catttgtaaa ataaagacct ataaaagatg agaccaacca
     7141 acctcatctt ttttcgtatc aaatctaagc aattttagaa actttatggc atactagact
     7201 tatcaatgaa agcgaggtaa tctcatggaa caatttttgg ataacatcaa agaccttgaa
     7261 gtcactacag ttgtgcgtgc gcaagaagct cttgataaaa aagaaactgc aaccttcttt
     7321 atcggtcgca aaacttgccc ttactgccgt aaatttgcag atacattgtc aggtgtcgta
     7381 gctgaaacca aagctcacat ttacttcatc aatagtgaag aaccaagcca actcaatgat
     7441 ttgcaagcat tccgctcacg ctatggaatc ccaactgtac caggttttgt tcatattaca
     7501 gatggacaaa tcaacgtccg ttgcgactct tcaatgtcag cacaagaaat caaagatttc
     7561 gcaggattgt aaaaaatcag ctaacaagtc ttaaaatcac aaaaacgcat aatgtaaggt
     7621 gcaaaaacct tgatattatg cgttttattg ttcctaaaac agttacatca gcatttcatt
     7681 acagaacatt ttaattttgg aagtcaaatc aatttttaac aatgttttag aaatcacagt
     7741 gggctattcc atattcaatt atccatattc aatcgctatt atcgttttcc tagattacaa
     7801 aaaaactacg ttttgctaat caaaacgcag tttcagccac tattttatct tactgtcctt
     7861 cttttacttc tttggttgct acatcttctc caaaccattt ttggctgatt tcttggaact
     7921 tgccgtcctt gtaaagacta gaaaaagctt catttatctt cttaaccaag gttgtatctt
     7981 ccttacgggc tccaaccgca aaagcttctg tttctagtcc aactgtaaag acattataat
     8041 cgtttaaaac accttctgct tctaaataat agtttgcata gacacggtca atcaatagac
     8101 catcaattcg atcgtttttc aaatcaatca aggcttcatt aaaggtttgg tattgattcg
     8161 cttccttatt agcgacaata ttcttcaaaa tttctggatt tgcttcaaag tccgcataac
     8221 cagatgaacc agcttgagct cctaatgtct ttccagtcat atcctttgca gtcgtgatac
     8281 cagatgattt cttcgtaacc aatacctgct cattcttcat atatgagtta ctgaaagcca
     8341 ccttttcacg gcgttcgtct gtagcggaat agccattcca aatcagatca atcgttcctt
     8401 ttgtcaattc agcttctttc aaatcccaat caatcggttg ccaatttacc gtgattccgt
     8461 atttttcaaa aacagctgta gctaaatcaa tatcaaatcc tgcataagaa ccatctttct
     8521 gagcaaatcc cattggaaca aaagtactat caaatccaat agtaatagac ttgttagact
     8581 ggtactttga ccaattatct ccactagttt cgctagaatt tttcccacaa gctactaaga
     8641 acaaagcagt catcagactg actaatacaa gcatccattt tttcatccat ttttcctcct
     8701 atttaggttc tactttcaat aatacatctg cgatattttc agcaaactgc aaatcatggg
     8761 taaccacaat ctgggtcatc ccaagttccc tattttgcaa gattagcttc tccacttcca
     8821 aacgtaattc tggatccagg gcagaagttg gttcatcgta gccaatgatt tctgggtcaa
     8881 tcatcatagc acgcgccaaa gccacccgct gcttttgccc accagatagt gagaaaggat
     8941 aggcctctgc gtgtcctcct agtcctaact gttccaagag ttcactcgcc ttcttctcag
     9001 cctcttcctg cttcattccc atggtcttca caggcgataa agtcaaattt tccagaactg
     9061 atagatgagg aaatagttga aaatcttgga agacaaatcc cagtaggttg cgcttctgca
     9121 attcatccag ctctaaaggt tgtccattat aaaagatttg ccctgaatca atggtttcaa
     9181 gacctgcaag catacgtaaa agagttgtct tacctccacc agaaggtcca acgatagcca
     9241 ggatttgctt ttcaggaata cttagactga aatcagacag gatttgtttg tctccaaaga
     9301 ctttattgat atttcgtaat tctaacatag ctgcccccta tctataataa ctgtactttt
     9361 tctcaacttt tttggaaata attgtcacaa tcccaattaa aatcaagtaa atggctcctg
     9421 ccaagaacat aggaaccaga ctagcatcgc ggttagcagc tgttcgacta gccaagataa
     9481 ggtctgaaat tccgagagca tagaccaaag aagtatcctt gaccaaactc ataacttcat
     9541 taaagacact aggaagaacg atcttggtca cttggggcaa gataatatag cgcactctgt
     9601 caaaagggct aaacttcaag accttggcag cctcatactg tcctcttgga atagtgtcaa
     9661 ttcccccacg gaaaatttct gcaaagtaag ctgcatagtt gagaacaaag gcaataatag
     9721 ctgcaggaag gcggtctaaa cgaatcccaa tacttgggag cacataatag ataaaaatca
     9781 gttgcaagag taagggggtt cctcgcataa cccagatgta aatgttaatc agataatgga
     9841 ggggcttcca atggacttgc aaggcaaagg caatcaaaac gcccaaggga atcgaaaata
     9901 gcaagaccag tgcaaagacc tgtacagtcg tgctcgcacc attcagtaaa ctcggtaata
     9961 tctcaaataa ataagacata ctgcacctcc taaaagtaat tgttcctatt atagcatgaa
    10021 taaacaagat taccaagtat ttttgtaaaa agattctacc atatacaaat aaaaaccgta
    10081 gctatcaaaa gagataaact acagttctat atgtttaaac attcttacaa tctctcaaac
    10141 agttcttgca tttgcacatc atctggaacc agttttaagt aagtgtgagc atggactttt
    10201 gcttcttcaa aatgtcccaa ttcacgcaag agatagatat agtgttccag aaattctgga
    10261 ttgtccttca aatctcctgt caactcttga taatgctcat aagcagtatc caaatcgtcc
    10321 atttcttgat aagaacgagc aatcatccac ttggtcaaaa gattttctgg ctcctcactc
    10381 tgcaagtcta gaatatcctc ataacgctcc tgctccagat aaatagtggc taaacgaagc
    10441 aagatttctt ctgtatcctc agcgtcttct tttgcagtaa ggagataatt ttctgcacca
    10501 ctagcatcat gcaattcata agaaaattgt gaagcagcta gcaagaggcg agtttcaaag
    10561 ggatttttct ctaatccttg cttagcgata cgcagggctt cttgaacttg atgttcctta
    10621 tgtaaagcct gactgtaccc atactcatag ccttcaaagt caggagaaat ggtatcaagc
    10681 tgcttaaagt agagggtggc tttttgatat tcttcttgat caaaataaag actggccaac
    10741 tcaaaagctg ttaagtcatc gtattctaac tccagggctt tttctaaaaa ctcagtagcc
    10801 gtttcaaatt tccctaactg agcataggca aagccaattc gttgataggt ggaaatgccc
    10861 gtttgctcat aaatcgagcg attatctaac tgggcatagg cttgaatagc cgcttggtaa
    10921 ttttccaact cactatccaa ctctgccaaa cccaatatca agagagaatc ctctgagtag
    10981 gtcaaggcct ccaataattt ctcacgtgcc acatctgtca aaccttccag ctggtaaagg
    11041 tctgccttca aaaccaaaga cgagacatac cagtcactgt cagcttggat ttcctcaaga
    11101 taggtaaagg cttcttctat ttgaccatcc tcgctagcaa ttgcagctag attaagatga
    11161 acctctggaa aatcctctac aattttcagg taaatttcct tggcctgagg atagaaacca
    11221 atcccttcaa gataagttgc caactcatac agaagattac ttgaatcatt ttctaaagct
    11281 ttggcgaaat aatgctcagc cttagttaaa tcttgctcct ccaaagcctg taacatctgt
    11341 tgactattgt tcactcttga cttcctcacc ctctaattca tatagaccac tgactacctt
    11401 ataccattcg aaaatggctg aaatgacaac cttagcagag gcataaaccg gaataccaag
    11461 taaaactccc cagataccaa acatagatcc tgaagttaac aaaacaaaga gaacattaat
    11521 agggtggatg tttaattgac ttcccaaaat caatggagag acaaaacggc cttcaatagt
    11581 ttgttctacg ataaagacaa tcactacttt caaaagcatg actggaccag caatcaaacc
    11641 caatactaga gcaggaagca tggctagaaa gctaccaaga taagggacca gatttaaaat
    11701 accagcagta acccccagcg taaccgcata gcgtagacca ataatcttga agaagatgat
    11761 aaacattact gctacaataa tagccactgt cacttgccct cgaacatagt tggacaactg
    11821 ttgattcaca tctgatagaa cttgtccaac aggttccttc aattttcttg gaatgaattg
    11881 ggtcaaatag ttacgcaagc ctttcccatc acgcaagaga taaaagagca tgaaaggaac
    11941 gataatcaag gcaacaatca cttgagaagc tccgctaata aaggcactca cccagttgac
    12001 tgcctgagat gaaaccttac ttgccaaaac tgtagcctgg ctagaaaaat tggtcaaaac
    12061 ttgctctaat tgaggtctga aatcatctgg caggtgctgg gctaccaatc cattaacaat
    12121 cctatctata tcttctaagt aaacaggaac gtttcttgca aaggtcaaaa cctgacgttg
    12181 cagatttgga atggcgactg ccaagcccca aatgataaag agagcgatga taacaaagac
    12241 aatagtgata gctataacac gattaacctt atgcttctcc atccaatcaa caataggatt
    12301 caacaaataa tataacaaac cagacaaaat gactggcaac atcacaactg ctaaaaagtc
    12361 taaaacaggt gaaaatagaa aactaatctt acttaaaata aaaagattca gtcccaataa
    12421 taaggttact aaaaataccg taattgcctt gttatctaaa aaccacttga aaaaccaaga
    12481 taggctaaaa tgtttctctt tttgctccat aaatacttcc tttctattgt tctctcatta
    12541 taccattttt gaaacaaaat aactcttttt taaagtgctt acatctaatt ggatatcttt
    12601 aatttacttg tttttgtgat ataatagtct tatctttaca cagaggtata gtaatgaaag
    12661 aaactgttta ttttggaact tatacacgtc gtacttctca agggatttac aaggcagact
    12721 ttgatacaga aactggtcag ctttcaaatc tagaactttt tgcagctgag ccaagtccaa
    12781 cctaccttgc ctttgaccag caccaacatt tatacactgt tggtagccaa gacgataagg
    12841 ggggaattgc agcctatcaa actgacggga ctgtgttaaa tcatgttgtt gaaaaaggag
    12901 ctccccactg ttatgttgct gtcgatgaaa agcgtgattt ggtttacgca gccaactatc
    12961 acaagggaca agtccttgtt tataaacgcc aggaagatgg tagtcttcta cttagtgata
    13021 tggatcaaca cagtggccaa ggtccacatg aaaatcaagc ttccccccat gttcactata
    13081 cagatttaac acctgaccac tatctagtga cctgcgactt gggaactgac caagtcatca
    13141 cctatgacct cgatcaagaa ggaaaattat ctaagctcta tacctatcac agcaagccag
    13201 gagcaggctc acgccatatc attttccata accactataa aatcgcttat ctcatttgtg
    13261 aactcaatag tactatcgaa gttttaatct acgatggcgt tggcgaattt gaacgtatgc
    13321 aggtcatttc aactttaccg gaagcttacg aaggctttaa tggaaccgct gctattcatc
    13381 tctctaaaga cggtaaatac ctctacgctt ctaaccgtgg ccatgattct atcgcagtat
    13441 ataccatcct tgcggacggt agcttagagt tattagaaat cgttccaacg catggtcaga
    13501 ctccacgtga ttttgatttg acacccgacc aaaaatttct cattgttgtc catcaagact
    13561 ctgacaatgc aactgtcttt aaacgtaatt gtgacaatgg tcgtctagca gaactctcca
    13621 acgacttcca tgttcccgaa gcagtctgca tccgttttgc tccttaaaac accataaaaa
    13681 atggttctat aatatttgta gtgggtaaat cccctatgga tattatggag ccta
//
LOCUS       DAVKLX010000054        13660 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKLX010000054 DAVKLX010000000
VERSION     DAVKLX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13660
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.054"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            1..625
                     /locus_tag="VW325_001776"
                     /pseudo
     CDS             1..625
                     /locus_tag="VW325_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(832..3090)
                     /locus_tag="VW325_001777"
     CDS             complement(832..3090)
                     /locus_tag="VW325_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9735120.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(3319..3549)
                     /locus_tag="VW325_001778"
     CDS             complement(3319..3549)
                     /locus_tag="VW325_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9735121.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3739..4422
                     /locus_tag="VW325_001779"
     CDS             3739..4422
                     /locus_tag="VW325_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9735122.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            4422..5156
                     /locus_tag="VW325_001780"
     CDS             4422..5156
                     /locus_tag="VW325_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9735123.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            5310..6167
                     /locus_tag="VW325_001781"
     CDS             5310..6167
                     /locus_tag="VW325_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9735124.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            6304..7125
                     /locus_tag="VW325_001782"
     CDS             6304..7125
                     /locus_tag="VW325_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEU9735125.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            7139..8011
                     /locus_tag="VW325_001783"
     CDS             7139..8011
                     /locus_tag="VW325_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9735126.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            8293..8976
                     /gene="rpiA"
                     /locus_tag="VW325_001784"
     CDS             8293..8976
                     /gene="rpiA"
                     /locus_tag="VW325_001784"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9735127.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            8990..10201
                     /locus_tag="VW325_001785"
     CDS             8990..10201
                     /locus_tag="VW325_001785"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9735128.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            10203..10745
                     /locus_tag="VW325_001786"
     CDS             10203..10745
                     /locus_tag="VW325_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU9735129.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            10761..11570
                     /locus_tag="VW325_001787"
     CDS             10761..11570
                     /locus_tag="VW325_001787"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9735130.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRTYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            12046..13026
                     /locus_tag="VW325_001788"
     CDS             12046..13026
                     /locus_tag="VW325_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9735131.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGCYLAQIAEVEAYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWNAKNGFWDVGLESRKLAVSGKIKHYVVDNDNVVTP
                     LIHNDRDIVTFTGNSRFKHRSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRF
                     FKKQALPRSSSQPSAEPMENIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHT
                     NFQSKPWWQVDLAKEETIRQINIYNRTDTAQDRLANFDVILLDSSGKELE"
     gene            13051..13563
                     /locus_tag="VW325_001789"
     CDS             13051..13563
                     /locus_tag="VW325_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9735132.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNGETAI
                     QVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         4075 a         2456 c         3052 g         4077 t
ORIGIN      
        1 gcttatttga ctgaaatagc ttctgaattt ggctgtcatc taactaccat ccactatgcg
       61 ctcaaagcta tgggttacac tcgaaaaaaa gaaccacacc tactatgaac aagacccaga
      121 aaaagtagcc ttatttctta agaattttaa tagtttaaag cacctagcac ctgtttagat
      181 tgacgaaaca ggattcgata cttattttta tcgagaatat ggtcgctcat taaaaggtca
      241 gttaataaga ggcaaagtat ctggaagaag atatcagaga atttctttgg ttgcaggtct
      301 aacaaatggt gagttaatcg ctccaatgac ttacgaagag acgatgacga gcgacttttt
      361 tgaagcttgg tttcagaagt ttctcttacc aacattaacc acaccatcgg ttattattat
      421 ggataatgca agattccata gaatgggtaa gttagaactc ttgtgtgaag agtttgggta
      481 taacttttta cctcttcctc cctactcacc tgagtacaat cctattgaga aaacatgggc
      541 tcatatcaaa aagcacctca aaaaggtatt accaagttgc aatacctttt atgaggctct
      601 tttgtcttgt tcttgtttca attgactata gcagattgaa ataagatgag aacaaatcga
      661 ttgggaaagt aaaattaatt tctataaatg ttttagcaat tgtttcgtac tatttcagat
      721 tcagtctact atatatcata atggacaaag ggcaagctgt caagaaatta cagttatttt
      781 agtaaaaaaa gaagcagaaa aatttcattt tctactcctt ttctatagga tttatttttc
      841 tttttcagaa cttttaactt cagctttttt ggcagattta atctgaatgt ttcccttgct
      901 agtcataact gctttgagat ctttttcgct tggattttca aggtagtagt cagtgattgt
      961 gtcctcaata tagtcttgaa tagtccgacg aagtgggcgt gctcccattt ttggatcata
     1021 acctaggtca accaactttt ccttgacctt gtcagttaca tccaaacgaa tgttgttgct
     1081 agagaggcgc ttgttaacat ctgctagcat gagctcgaca atctgaagga ggttatcctt
     1141 gctgagagcc ttaaattcga taatgccatc aaaacggttc ataaactctg ggctaaagaa
     1201 gttaccgagt tcaccgagga cagaattggt acgtccttct ctagcagcac caaatccaac
     1261 gctagcttcg gtctttcctg tacctgcatt tgaggtcatg ataatgatgg catccttgaa
     1321 gctaacggtg cgtccttgcc cgtctgtcaa acgaccatcg tccaagactt gaagaaacat
     1381 gtgcataaca tctgggtgag ctttttccac ttcatcgaga aggatgagag aatacggatt
     1441 gtggcgaact ttttcagtta attgaccagc ctcatcatag ccaacataac ctggaggagc
     1501 gccgaccaac ttagccacac tatgtttttc catgtattca ctcatatcaa agcgaatcat
     1561 actatcagca gaaccaaaaa gttcgatagc cagttgtttg gaaagttctg tcttaccgac
     1621 accagttggt ccaacgaaga ggaagcttcc gattgggcgg ttaggggtac caagtccgac
     1681 acgattacgg cgaatagcct tggcaatctt atcgactgca tcatcttggc caataacatg
     1741 agacttgaga tcttcggcta gatggatgag ttgagattgt tctttctctt tcaaatcacc
     1801 aacagggata ttggttttct gctcgataat gtgctcaata gttttctcgc tgatgatagg
     1861 agtatcctgg tctgtgatct ttttcttttg catttcctta tacttggcaa tctggtcgcg
     1921 gaagtaggcc gccttctcaa aatcttcttc tcgtgtagct tgagacttga gattttcagc
     1981 ctcaatcaag cgctgatcaa ttactttagg atccacaaaa ttcaaggtca agttcatctt
     2041 agaaccagct tcatctagga ggtcaatggc cttgtcaggc aagaagcgat cttggatgta
     2101 gcgattggaa agagttgcag ctgcttcaat cgcagcatcg gtatattgaa cgtggtggta
     2161 atcttcgtat ttcttttgaa tccctttgag aatagtgatt gtttcatcca ccgttggttc
     2221 atcgacttta acaggctgca tacgacgctc gagggcagca tccttttcaa tgatacggta
     2281 ttcattgagg gtagtagcac cgactagttg cagttctcca cgagcaaggg ctggcttgag
     2341 gatatttcct gcgtccatat taccatcact cgcagaacca gcaccaacaa tttcatggat
     2401 ttcatcgata aagaggatga tgtcttcacg tttgcgaatt tcttccatga gtttttgcat
     2461 gcgttcttca aattgtcctc gaatccccgt tccttgaact aagctaacca catccagacg
     2521 gatgacttgt ttaccttgga gtttatgtgg cacatcgcca tcgacaattt tctgagctag
     2581 accttcgaca acggccgttt ttccgacacc aggttcaccg ataaggacag gattattctt
     2641 ggttctacga ttgagaatct cgatgacacg gataatctca tcgtcgcgcc caataacggg
     2701 gtcaatgtct ccacgacggg caatttcagt tacattaata ccaaattctt ccagcaggcc
     2761 tttttcttgg ctaggtggcg gagtttgagc agatccacga ttttgggaac cataaccgcc
     2821 gtttccaccg taacctccac ctgattgggt tgggggaata ggaggagtat tgctagaagg
     2881 tctgaaattg tttagatcat tgaagaaatc accaaaggga tcgaagtcac gattgttcag
     2941 atccgtcata cctttgaaga ggctattgtt aggatctgtt ttgataatct tatagcagtt
     3001 ttgacagagg tcaatttgtt tttgttttcc attgagattg gtgtaaagat gaattgttga
     3061 gtcgttaatt ttacagtttt gacaaagcat acatctacct catttctttc tttagcctga
     3121 cttgtttaaa ggtcaaaaat agtcaaattc tatactctca ttataacagg attggggtgt
     3181 aaagcaagta aaaagattga agctttgaga agttgttaca atgaaaattt ttgtgaattt
     3241 ggactataaa aaaatcctat ttgtgagaca aaaaggattt tacttagaca tgcagggaca
     3301 atcccaactt gttttagatt agtagagaag ttcgtaaatt tccattgcga ttaagtcaat
     3361 gctatcaaac gtatacgttt cgcgatcttc tacgtcacga agggtaaaga ttgggccgtt
     3421 ttcttcgtcg tggctgtacg caacttctgc aacaacaact ccttcgcgtt caaaattacg
     3481 ttttaaattt ccgccatcgt ttgccattgc ttcaaggcgg ttgatgattc taaccaaatg
     3541 tgattccatt ttgattctcc ttcatttctt atcgttacta ttttaccata aaggagggca
     3601 acttgactag aaaaaactaa gatttctcgc taattctgct agtttaaaga tttttaaata
     3661 aattggataa aaaatttaaa ttttaattca aaacatgtta taatcataca gtattctatt
     3721 ttagaaagca gtgtgactat gaatttttct tttttaccta agtatttacc ttattttaac
     3781 tatggggctg ttgtgacgat tcttatttct atctgtgtta tctttttggg aactattttg
     3841 ggtgttgtct tggcttttgg gcaacgttca aagtttaaac cgcttgtttg gttggccaac
     3901 ttgtacgttt ggattttccg tgggacaccg atgatggttc aaattatgat tgcctttgct
     3961 cttatgcata tcaatgctcc gactattcag attggaattt taggtgttga tttttcgcgt
     4021 ctgattccag ggattttgat tatctctatg aatagtggtg cttatgtttc ggagactgtt
     4081 cgtgccggaa tcaatgcggt tccaaaaggt cagctagagg cggcttattc gctagggatt
     4141 cgtcttaaaa atgcgatgcg ttatgtgatt ttgccacaag cagtcaaaaa tatcttgcca
     4201 gcattgggga acgaatttat caccattatc aaggacagct ccctcttatc agctattggg
     4261 gtcatggagt tgtggaatgg ggctacaaca gtttctacaa caacctatct acctttaaca
     4321 ccacttttat ttgcagcatt ttactacttg attatgacct ctattctgac agtagccttg
     4381 aaagcttttg aaaaacatat gggacaagga gataagaaat aatgacagaa accttgataa
     4441 aaattgaaaa tttacataaa tcttttggaa agaatgaagt attgaagggc atcaacctcg
     4501 agattaaaag aggagaagtt gtcgttatca tcggtccttc agggagtggg aaatctacct
     4561 tgcttcgctc tatgaatttg ttggaggaag caaccaaggg gaagcttatc tttgagggag
     4621 tcgatattac ggacaagaag aatgacctgt ttgccatgcg tgagaagatg ggcatggttt
     4681 tccaacaatt taacctcttt cctaatatga ctgtgatgga aaatattacc ttgtcaccta
     4741 tcaagactaa aggtgaaagc agggaagttg ctgagaagag agcccaagag cttttggaaa
     4801 aagttggttt gccagataag gcagacgctt atccacagag tctgtcgggt gggcagcaac
     4861 aacggattgc catcgcgcgt gggttggcta tggaaccaga tgttttgctc tttgacgaac
     4921 caacttcagc cttggatcct gaaatggtag gtgaggtatt ggctgttatg caagaccttg
     4981 ccaagtcagg gatgactatg gttatcgtaa cacatgagat gggatttgcc cgtgaggtgg
     5041 cagatcgtgt tatctttatg gcagacggtg tggttgttga agatgggaca cctgagcaga
     5101 tttttgaaca aacccaagaa caacggacta aagatttctt gagtaaggtt ttataatcta
     5161 ttttaaaatt tcaagacaag attagtgaaa agttcaacca gagctttttc ttatagtttg
     5221 aaactatagg ataacctagg aaagaagtgt tagagggcac tagcagtttt tggtataatg
     5281 gaagatattt gaaagaaaag agaagtgata tgacacagat tattgatggg aaagctttag
     5341 cggccaaatt gcaggggcag ttggctgaaa agactgcaaa attaaaggaa gaaacagctc
     5401 tagtgcctgg tttggtagtg attttggttg gggataatcc agccagccaa gtctacgttc
     5461 gcaacaagga gaggtcagct cttgcggctg gttcccgtag cgaagtagtg cgagttccag
     5521 agaccattac tcaagaggaa ttgttagacc tgattgctaa atacaatcag gatccagctt
     5581 ggcatgggat tttggttcag ttgccattac caaaacacat tgatgaagag gcggttctat
     5641 tggctattga cccagaaaag gatgtggatg gtttccatcc tctaaatatg ggacgccttt
     5701 ggtctggaca tccagtcatg attccatcaa cacctgcagg aattatggaa atgtttcatg
     5761 aatatgggat tgaattagaa ggtaaaaatg cggttgtcat cggtcgttct aatatcgttg
     5821 gaaaacccat ggctcagctt cttttggcca agaatgcaac agtgactttg acccactcac
     5881 gcacccataa tcttgctaag gtggctgcta aagcagatat tctggtggtc gcaatcggtc
     5941 gtgccaagtt tgtgactgct gactttgtca aaccaggtgc ggtagtcatt gacgttggga
     6001 tgaatcgtga tgaaaatggt aagctctgtg gggatgtgga ttatgaggca gttgccccac
     6061 ttgctagcta tattacgcca gtccctggag gtgtcggccc tatgaccatt actatgctga
     6121 tggagcaaac ttatcaagca gcacttcgga cattggatag aaaataagag aaaaatcgct
     6181 gaagagagtg tattttctat agctatattt aaaatggcag aaatgaatat taaattttag
     6241 atataagatt acaaaaggag gtctgcgcct cctttttgtt gtataatgga gtgaggtgat
     6301 tagatgattt taaaaattta taatagggaa tatagtttac aatgggatgg aatatactac
     6361 ctagcactaa ttgattatcc aaatattcaa gagtgggaat tagaaaaaat tgctaaattt
     6421 atagcttacg aaaaacttca taaacgtcaa acaagtattg agtgtgctga ttcttgttta
     6481 aaaaaagaaa ttttagatta catctgtcag catccctttc tgccaccatt tacccctaca
     6541 gataaaagag tagcctcgac ttatgatcta cataagaggt tagtgacttc agactactgt
     6601 agtcatacta cgactataga tgcagcgatt tctatcttta aaacaggtca gctcttatct
     6661 gctgtgaaag cctttaggcg agatgctgaa gagttggttt tggatagtag aaatgctgca
     6721 tcggatccga tagattattt tgattatgtc atgttagggt ggtcaaatac aagttctggt
     6781 tatcgattag cgatggagcg tttattaggt cgagctcctt cagagaaaga attacaagac
     6841 aagtttattc ctggagtgag ttttcatttt atctatacag atttgattaa agttactggt
     6901 tatatttttg atggctacca tgttgcaaaa attaaggaca tgttgaattt atttagtgag
     6961 ttgtatattt gtattattcc aactcataat aagagccaat ttgaaaatat tattccaact
     7021 aaaatacaag atagggtgca ttatcttgac tatgctggag aagacttaga agagtggact
     7081 aagaaagtct atcaagttgt tttaaaacaa tcagataaag gatagttgag gaagaacgat
     7141 gaaagtgatt aatcaaacct tattagaaaa agttattatt gaacgttctc gttcaagtca
     7201 taaaggagat tatggtcgtc tgctgctgct tggtggtact tatccttatg gaggtgccat
     7261 catcatggct gctttagcag ctgtaaaaag cggtgcagga ttggtgaccg ttggaacgga
     7321 cagggaaaat atcccggctc tgcacagcca tttacctgag cctatggcct tttctcttca
     7381 agaccagcaa ttgttaaaag agcaattgga gaaggcagaa gttgtcttgc tggggcctgg
     7441 tttacgagac gatgcttctg gagaaaatct agtaaaacag gtctttgtta atttaagcca
     7501 aaatcagatt ttgattgtag acggaggtgc cttgaccatt cttgctagga caagtttgtc
     7561 atttccctca agccagctta tcctaactcc tcaccaaaaa gaatgggaaa aattatctgg
     7621 aattacgatt gaaaagcaaa aggaagatgc aacggctagt gttctgactt ccttccctca
     7681 aggaacaatt ttggtggaga aaggcccagc tactcgtatt tgggaagttg gccaatctga
     7741 ttattaccag ttacaggttg gtggtcctta tcaggcgact gggggaatgg gagatactct
     7801 ggctgggatg atcgcaggat ttgtaggcca attccgacag gccagtctct acgagcgtgt
     7861 ggcagtagcg acccatcttc attcagccat agcccaagaa ctatctcaag aaaattatgt
     7921 ggtcttaccg acggaaatca gcaggtatct tcccaaaata atgaaaatta tttgtcaaca
     7981 agagagggta agcaaagata aacttgttta aaatagacag ataaatctac ttccttctct
     8041 taatttatga ttcttgaaat gcgcctgctt taaggctaaa ctagacgtat caaagttaag
     8101 agtttgtttt tgtaactgga aagagatgaa caaatcaaat acataaaaga taaactcatt
     8161 tttattcgtt tggtaaaaat aaaatgcata tttttaaaga aagattgatg aaaaatgtag
     8221 atttgatgtt tttacttgaa taaaaagcgg tttcatagta aaataagaac tgagaatgat
     8281 ggaggtaaat cggtggaaaa tctgaagaaa atggcaggta tcaaggctgc tgagttcgtg
     8341 agcgatggaa tggtcgttgg acttggaaca ggctcgactg cctattattt tgtcgaagaa
     8401 atcggtcgtc gtatcaagga agaaggattg cagattacag ctgtaacaac ttctagtgtg
     8461 accactaaac aggcagaagg ccttaatatc ccgctcaagt ctattgacca agtagatttt
     8521 gtcgatgtga cggttgatgg cgcggatgaa gtggatagtc agtttaatgg aatcaaaggc
     8581 ggtggtggtg cccttctcat ggaaaaggtg gtcgcaacac catcaaaaga atacatttgg
     8641 gtggtggatg aaagcaagct ggtcgaaaaa ctaggtgctt ttaaattgcc agtagaagtg
     8701 gttcagtatg gtgcagagca ggtctttcgt cattttgaac gagctggcta caaaccaagt
     8761 ttccgtgaaa aagacggcca acgttttgtg acggatatgc agaattttat cattgacctc
     8821 gccttggatg tcattgaaaa tccaattgct tttggacaag aattggacca tgtcgttggt
     8881 gttgtggagc atggtttatt caaccaaatg gtggataagg taatcgttgc tggacgagat
     8941 ggagttcaga tttcaacttc aaaaaaagga aaatagaagg gggcataaga tgtctaaatt
     9001 taatcgtatt catttggtgg tactggattc tgtaggaatc ggtgcagcac cagatgctaa
     9061 taactttgtc aatgcagggg ttccagatgg agcttctgac acactgggac acatttcaaa
     9121 aacagttggt ttgaatgtcc caaacatggc taaaataggt cttggaaata ttcctcgtga
     9181 aactcctctt aagactgtag cagctgaaag caatccaact ggatatgcaa caaaattaga
     9241 ggaagtatct cttggtaagg atactatgac tggacactgg gaaatcatgg gactcaacat
     9301 tactgagcct ttcgatactt tctggaacgg attcccagaa gaaatcctga caaaaatcga
     9361 agaattctca ggacgcaagg ttattcgtga agccaacaaa ccttattcag gaacggctgt
     9421 tatcgatgat tttggaccac gtcagatgga aactggagag ttgattatct atacttcagc
     9481 tgaccctgtt ttgcagattg ctgcccacga agacattatt cctttggatg aattgtaccg
     9541 tatctgtgaa tacgctcgtt cgattaccct tgagcgtcct gcccttcttg gtcgcatcat
     9601 tgctcgccct tatgtaggtg aaccaggtaa cttcactcgt acggcaaacc gtcgtgactt
     9661 ggctgtatct ccattttccc caactgtttt ggataaattg aatgaggctg gtatcgatac
     9721 ttatgctgtg ggtaaaatca acgatatctt taacggtgct ggtatcaacc atgacatggg
     9781 tcacaacaag tcaaatagtc atggaattga tacactattg aagactatgg gacttgctga
     9841 gtttgaaaaa ggattctcat tcacaaacct agttgacttt gatgcccttt acggccatcg
     9901 tcgtaatgct cacggttacc gtgattgctt gcatgagttt gatgaacgct tacctgaaat
     9961 tatcgcagct atgagagaga atgaccttct cttgattact gcggaccatg gaaatgaccc
    10021 aacgtatgca ggaacggatc acactcggga atatattcca ttgttggcct atagccctgc
    10081 ctttaaagga aatggtctca ttccagtagg acattttgca gatatttcag cgactgttgc
    10141 cgataacttt ggtgtggaaa ctgctatgat tggggaaagt ttcttagata aattggtata
    10201 agatgacgcg ctatgctttg ctggtgagag gtatcaatgt tggtggtaag aataaggtcg
    10261 tcatggcgga gcttcgtcaa gaattgacaa acttgggact ggaaaaggtt gagagctaca
    10321 tcaatagtgg caatattttc tttacttcga tagattccaa agcccaattg gttgaaaagc
    10381 tagagacttt ctttgcagtc cattatccat ttattcagag cttttcttta ctgagtctag
    10441 aggactttga ggcggaactt gaaaatctac cagcttggtg gagcagagac ttggcacgaa
    10501 aagattttct cttttacact gagggtttgg atgtggacca agtcatcgcg acagttgaaa
    10561 gtttagagct gaaagatgaa gtgctttatt ttggaaaact tgggattttc tgggggaaat
    10621 tttctgaaga atcctattct aagactgcct atcataagta cttgctgaag gtgcctttct
    10681 accgccacat tactattcgt aatgctaaaa cctttgacaa aattggtcaa atgctaaaaa
    10741 aataataaag gagacacaca atgacatttt taaacaaaat ccatgaaact gctactttcc
    10801 tgaaagaaaa gggaattgca gcccctgagt tcggtctaat ccttggatca ggacttggag
    10861 aattggcaga agaaatcgaa aatccagttg tagtagacta tgctgagatt ccaaactggg
    10921 gccgttcaac agtagtcggt catgctggta aattggtata tggtgaactg gcaggtcgca
    10981 aggtcttggc tcttcaaggt cgtttccatt tctatgaagg gaatcctctg gaagtggtga
    11041 ctttcccagt tcgtgtgatg aaagttcttg gatgtgaagg tgttattgta accaatgcag
    11101 ctggcggtat cggatttggt cctggtacct tgatggctat ctcagaccat atcaacatga
    11161 cggggcaaaa tccattgatg ggtgaaaact tggatgactt tggcccacgt ttcccagata
    11221 tgtctaggac ctacacacca gaataccgtg ccactgccca tgaagtggct aaaaaactta
    11281 atatcaagct tgatgaaggt gtctatatcg gagttactgg tccgacttat gaaacaccag
    11341 cagaaattcg ttcctataag acactgggag cagatgcagt tggtatgtct acggttcctg
    11401 aagttatcgt ggcagcccac tctggcttga aagttctggg aatttcatgt atcactaact
    11461 ttgcggctgg tttccaagaa gaactcaatc acgaagaagt tgtagaagtg actgaacgtg
    11521 ttaaaggtga tttcaaaggc ttgcttaaag cgattcttgc tgaattgtaa gaaaaaagat
    11581 ttaaaagggg gagtgcctct gtcttttcag gattgactgc ctatccggat taaagaagaa
    11641 acagaggaat actatgagct tcttcctgct cttataactg aaagaagcgg aagaataggt
    11701 atgtctgatc tgatagccag cattgtgaaa gacaagattc taggatacta gcattagctt
    11761 cctagccaag tagactagta tgataaggag agatgagaat gaattgactt tctgaatttc
    11821 tcagtcttat catatatagc acaatgagat ttcgcttgag tctgcttgta aataaacgaa
    11881 aagaaagata agaaataatg aaaattggtc aacgaattat gcgctttggc ataaaaaatt
    11941 aagtatcgga gttgtatctg ttgtagtcgg ctttgatttc tagctccagc tggaatttca
    12001 gccaatgaag taaagcaaga tgtaacatct gaagtggtaa taggtgtgct agattctaag
    12061 gaggaattga aagagtcaga aaatgatgct ccaaaactag aaactcctct tagagaggag
    12121 ccaagactag ctcctcaaac gcttccggaa gcaagtgaag ttcttgaaaa caaaagggaa
    12181 gagtcaaaag tagagataac agagccagct caagcggatg atatccgcaa ggttgttggg
    12241 gaattagcca aggatataag tattactaag ttgtatatga caggtcattc tcttggatgc
    12301 tacctagctc agattgcaga ggttgaagct taccaaaaat atcctgattt ttataaccat
    12361 gtattgagga aagtgacaac tttcagtgct cctaaagtga ttacttccag aactgtttgg
    12421 aatgctaaga atggtttctg ggatgttggt ttggaaagtc gtaaattagc tgttagtgga
    12481 aaaattaagc attatgtggt tgataatgac aatgttgtga ctcccttgat tcataatgat
    12541 cgtgatattg ttacatttac aggtaattca cgctttaaac accgttctcg tggctatttt
    12601 gaaagtccaa tgaatgatat tcctaacttt aatattggta aacaagctac cttggataaa
    12661 catggttatc gtgatccgaa attggataaa gtgcgattct ttaagaaaca ggctctgcct
    12721 cgatcttcta gtcaaccaag cgctgaacca atggaaaata ttgccttagg aaaacaggtt
    12781 actcaaagtt cgacagcttt cggaggagat gctagaagag ctgtggatgg caaagtcgat
    12841 ggtaactatg gtcacaattc tgtcactcat acaaacttcc aatctaagcc ttggtggcaa
    12901 gtagatttgg ctaaagaaga aaccattcgc caaatcaata tttacaaccg aacagacact
    12961 gcccaggata gattggcaaa ctttgatgtc attcttttag acagttctgg taaagaactt
    13021 gagtgaaaac gtataacatc tcctaaagat gtgtcagcac aaattacgat taaccataaa
    13081 aaagcgcgct atgttcggat tgagctagaa ggctataatg ccctcagtct tgcagaagtt
    13141 gaagttttcc gctttatagc tacgaatggt gaaacggcga tacaagtttc taagtcagtt
    13201 caaccaatca gtcagactcc tgtgaaggat aaaacattga caattcaaca cagtggagct
    13261 tacattgccc gctactccat aacttgggaa gaagttccag tagataaaga tggaaaccaa
    13321 gttgttcgta gtcattcttg ggaaggaaac ggtcgcaacc agactgcagg ttttgtcctc
    13381 aacctcccaa tcaaagaaaa tatgagaaat ctgcgagtta agattgagaa aaagacgggc
    13441 ctactatgga atagatggca aacaatctat gaaaacagac caattttagc tcaaccccac
    13501 cgtaaaatta cccattgggg tacgacattg aattccaagg tgagtgacga tgatgtcttg
    13561 taatctgatg gtacaatgat agttagtttg tctagtttat aagaaagtac tacctgagct
    13621 tgaataggac tcaggtagct ctctgtgaaa gaacaaaatt
//
LOCUS       DAVKLX010000055        13092 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKLX010000055 DAVKLX010000000
VERSION     DAVKLX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.055"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(75..272)
                     /locus_tag="VW325_001790"
     CDS             complement(75..272)
                     /locus_tag="VW325_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9735133.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(347..1123)
                     /locus_tag="VW325_001791"
     CDS             complement(347..1123)
                     /locus_tag="VW325_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9735134.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGKEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            1245..1991
                     /locus_tag="VW325_001792"
     CDS             1245..1991
                     /locus_tag="VW325_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9735135.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            2004..2984
                     /locus_tag="VW325_001793"
     CDS             2004..2984
                     /locus_tag="VW325_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9735136.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3179..3499
                     /locus_tag="VW325_001794"
     CDS             3179..3499
                     /locus_tag="VW325_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9735137.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            3545..3853
                     /locus_tag="VW325_001795"
     CDS             3545..3853
                     /locus_tag="VW325_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9735138.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3870..5168
                     /locus_tag="VW325_001796"
     CDS             3870..5168
                     /locus_tag="VW325_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9735139.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGTIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            5319..7757
                     /locus_tag="VW325_001797"
     CDS             5319..7757
                     /locus_tag="VW325_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9735140.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7776..8444
                     /locus_tag="VW325_001798"
     CDS             7776..8444
                     /locus_tag="VW325_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9735141.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            8462..9550
                     /locus_tag="VW325_001799"
     CDS             8462..9550
                     /locus_tag="VW325_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9735142.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            10005..12506
                     /locus_tag="VW325_001800"
     CDS             10005..12506
                     /locus_tag="VW325_001800"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9735143.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGDVKEAAYTEDGLHVNSDFLDELNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIDSLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            12645..>13092
                     /locus_tag="VW325_001801"
     CDS             12645..>13092
                     /locus_tag="VW325_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735144.1"
                     /translation="MNRLVNDSRKCEILLFKISIIVVSVIVLVMSIVGAYYFKNCLWD
                     KESLNLTLTLDTLFAVFITFFSFSNDKSSNIFGISPNEIIRTQSEVYQFLFTKSFATL
                     LIVTNLLSIIHYFGAKNLIVSFNETYPRIQSSVGIMWLPLFGILIIY"
BASE COUNT         4249 a         2012 c         2674 g         4157 t
ORIGIN      
        1 tcctgataga gatgttcaca tcttatttca attcactata ttagagtaaa attctctaca
       61 aaaagaagaa tagcctattt tactattctt ccgagtgatt tcaatttctt tggggaaata
      121 tggagatact ttttaaatac tttggcaaaa tatttatagt ctgaaaatcc aacttggtca
      181 gagatagcgc ttaaagggga gttgggagat tcagccatct tatctatagc ttgtttcagg
      241 cgatattgta agatatattc atttagagtc atggggaagt cttctttgat aaccttatat
      301 agatgcttgt tatctgcaaa tagctagagc atttcaagga aatgaactag aaataacaat
      361 taatatggtg gttcagaaat accttttgat aatcaataag atcttcagga tgaagtgcgt
      421 tgactccatc catttcatat ttccgattta ataaacgata tttgttttca ccaaattgat
      481 gaaaagggag tagttgaact tggtcgatat ttaatgagtt aaatagagta gcgaattttt
      541 ctgcatcctc taaactattg ttaaaattag gaataactgg gattcttaaa acgatagttt
      601 tattttgaga aaaagcataa tgaatgtttt taataatcat ttgattaaaa accccagtca
      661 cttttttatg ttttatagaa ttataatgtt ttaggtctgt gtagataaaa tccacatatt
      721 gaattaaatc aataaatttt tcatgatcaa caaaggcagt agtttcaatg gcagtgtgta
      781 tgtgatgttc tttagctgat tttaagatgg ctttagcaaa ttcaaactga gcaaatattt
      841 cacctcctga taaagttaaa cctccaccgg attcttcgta aaattctttg tcttttaata
      901 cctctgtaat aatttcttct acagtctttt ctttaccgac taaggtgaat ttctctcgtt
      961 gagcatcttt cattttttca ggtttcattc tttgagattc aggattagaa caccatggac
     1021 agcgcagagg acatcctttt aaaaaaacag ttgtacgaat acccgggccg tcatgaattg
     1081 aaaagtgttg aatattaaaa ataattcctt tagatatttc cataagagtt cctcctcttc
     1141 ataagtctat cctatcacaa aaacgaaaga aaaacaatta cgaacgaaag gttaatttgt
     1201 tgttttttgc tttttatttt ggtagaataa aacggaggtg taaaatgaaa agattggaac
     1261 aaattattaa attagtatca gaatatgaaa agatcgacgt taatacatta tcggaaaaat
     1321 taaatgtatc gaaagtaacg attagaaaag atttagataa attagagtca aaaggtttat
     1381 tacacagaga gcatggatat gctgtattaa atagtggaga tgacttaaat gtacgtttgt
     1441 caattaatta tgaaattaag agaaaaattg ttcaggaagc agtaaaattg gtgtcagata
     1501 atgaaacaat aatgatagaa tctggatcga cctgtgcttt acttgctgag gaaatttgca
     1561 agcaaaaaag aaatgttacg attgtaacaa attcattttt tatagcaaat tttgtgagag
     1621 cttatgattc atgtcgtatt attgttcttg gtggagagtt tcagaaggat tcacaggtta
     1681 ctgtgggacc tttattaaaa gaaatgatac agacttttca tgtgcatcaa gcttttgttg
     1741 ggacagatgg ttacgataaa gagatgggct ttacaggaaa agatttaatg cgcagtgagg
     1801 tagttcaata tatttcagca gcgtcggata aagtcattgt actaactgac tcaagtaaat
     1861 ttgataaaag gggtacagta agaagatttg ctttaagtca agtctatgaa gtaataacag
     1921 acgaaaaact ttctaaacaa aatatagcta cattagaaaa tgctgggata atggttaagg
     1981 tagtttcgta agaggttaag tgtatgaatc aagataggaa taaactgctt tctaaaattg
     2041 cttatctgta ttatattgaa aacttaaatc agtcacaaat agcagcaaaa ttaggaattt
     2101 atagaacctc tattagtaga atgttaacag aagcaaggaa tgcaggaatt gttaaaattg
     2161 aaatagagaa ttttgatacc aatatgttta agttggaaaa ttatgtaaaa gaaaaatatg
     2221 gtctggaaag tttagaaatt attccaaatg aatttgatga taatccaaca attttatctg
     2281 aaagaatttc tcaagttgca gcaggtgtcc ttaggaatct aattgatgat aatatgaaaa
     2341 ttggcttttc ttgggggaaa agtttaagta atttagtaga tttaattcac agtaaaagtg
     2401 tccgaaatgt tcacttctat cctctagcag gtggtcctag tcacatacac gctaaatacc
     2461 atgtgaatac actgatttat gaaatgtcta gaaaatttca tggagagtgt acatttatga
     2521 atgcaacgat tgtgcaagaa aataaattgt tagcagatgg tattttgcaa tcaagatatt
     2581 ttgaaaattt gaaaaatagt tggaaagatt tagatatagc tgtagtcgga attggtgatt
     2641 ttagcaataa aggaaagcat caatggttag acatgcttac agaggatgac tttaaagaat
     2701 tgaccaaagt taaaactgta ggagaaattt gttgtcgatt ttttgattca aaaggtaaag
     2761 aagtctatga aaacttacag gaaagaacga tagcaatctc tttggaagat ttaaaaaata
     2821 ttcctcaaag tttagctgtt gcttacggtg atacgaaagt atcttcgatt ctttctgtct
     2881 tgcgtgctaa tttagtaaat catttgatta cagacaaaaa tacaatttta aaagttttgg
     2941 aagaagatgg ggatttgact tttagagaga ttctaggtga gtgaaaatga tagactgatt
     3001 cagtttatcg tttttctttt tagttgattg cacatttgtg cttatataaa caaaaatagt
     3061 ttatctgttg tttttggatt gacaacttta ttatgtagtt gtattctata gttacaaaag
     3121 aaaattttaa aatttcaaat gaaaaaagct ttttacatag tgaaatgagg aggaatttat
     3181 ggaaattatt gttccagatc aaattatcat gggtttaatt ttatatgctg gcgatgcgaa
     3241 acaacatatt tataaagcat tagattacat aaagaacggt acatgtgaac gatgtgaaga
     3301 agaaatccag ttagctgatg cagccttatt agaagctcat aatctacaaa caaaattctt
     3361 ggcacaggaa gcgtctggta caaagacaga aattacagct ctctttgttc attcacaaga
     3421 tcatctcatg actagtatga cggagattaa tttaatcaaa gaaattatta gtttgagaaa
     3481 agaacttcat aaaaaataat actagagtat tatcattgtt attaacatag aggaggaaaa
     3541 cataatggtg aagattggtt tgttttgtgc agcaggtttt tctactggta tgcttgtaaa
     3601 taatatgaaa attgcagcgc aagctagtgg agttgaggca gaaatagagg cgttttctca
     3661 gtctaaatta gcggattatg cgccaaatat agatgttgca ctattgggtc cacaagttgc
     3721 ttatacatta gataaatcaa aagaaatttg tgataagtgt gatgttccga tagctgttat
     3781 tccgatgatg gactatggta tgttagatgg gaaaaaagta ttagatttgg ccctatcttt
     3841 gattagtggg taagaaaagg agatttatta tggcaaagat ggatgttcag aaaatcattg
     3901 caccgatgat gaagtttgtg aatatgcgtg gcattatagc tctaaaagat gggatgttag
     3961 caattttgcc attgacagta gttggtagtt tattcttgat tatgggacaa ttgccgttcg
     4021 aaggattaaa taagagcatt gctagtgttt ttggagctaa ttggacagag ccgtttatgc
     4081 aagtatattc aggaactttt gctattatgg gtctaatttc ttgtttttca attgcctatt
     4141 cttatgctaa gaatagcgga gtagaggctt taccagctgg agttctatct gtatctgcat
     4201 tctttatttt gctaagatca tcttatatcc ctaaacaagg tgaggcaatt ggggatgcta
     4261 ttagtaaagt ttggtttgga ggtcaaggaa ttatcggtgc tatcattata ggtttggtag
     4321 taggaagtat ttataccttc tttataaaga gaaaaattgt tattaagatg ccagaacaag
     4381 ttccacaagc tattgccaaa cagtttgaag caatgattcc agcatttgta attttcttac
     4441 tttctatgat tgtatatatt ttagcgaagt cattgactaa tggcggaaca ttcatagaaa
     4501 tgatttattc tgctattcaa gttccattgc aaggtttaac tggatctttg tatggtgcaa
     4561 ttggaattgc attctttata tcatttttgt ggtggtttgg tgttcatgga caatcggtag
     4621 taaatggagt agtgacagct ctgcttttat ctaatcttga tgctaataaa gctatgttag
     4681 cctctgctaa tctatcatta gaaaatggtg cacatattgt tactcaacaa tttttagatt
     4741 catttttaat tctatcaggt tcagggatta cgtttggtct tgtagttgcc atgctttttg
     4801 cagcaaaatc aaaacaatac caagccttag gaaaagttgc agcttttcca gcaatattta
     4861 acgtaaatga gccagttgta tttggatttc cgattgtcat gaatccagtt atgtttgtac
     4921 ctttcattct tgttcctgta cttgcagctg tgatagtata tggaactatt gcaacaggtt
     4981 tcatgcagcc attctcaggg gtaacattgc cttggagtac accagctatt ttatcaggat
     5041 ttttggtggg tggatggcaa ggagttatta ctcagctggt gatattagcg atgtctacat
     5101 tggtttattt tccattcttt aaagtacagg atcgtttagc ttaccaaaat gaaatcaaac
     5161 aatcttagag gtatttgtgt gttactgtta aactcacaca tttgtgctaa aaattagaga
     5221 gttaaaattt ttctagttaa aagcttgaaa atttctataa aaatcggtat tatattttcg
     5281 aaagaaataa aaatattttc gaaagaaagg tggttacgat ggtaaataca gaagtagcaa
     5341 gaacaacaat caagacagaa tattttggca gccttactga aaggatgaac aaatatcgag
     5401 aagatgtttt aaataaaaaa ccttatattg atgctgagag agcagttcta gcaacacgcg
     5461 cctatgaacg atacaaggaa caacctaatg tcctaaaacg tgcatatatg ctgaaagaaa
     5521 ttttggaaaa tatgactatc tatattgaag aagaatctat gattgcggga aatcaagctt
     5581 cttccaataa agatgctcct atttttccgg aatatacgct agaatttgtt ctcaatgagt
     5641 tggatctttt tgaaaagcgt gatggagatg ttttctatat tacagaagaa acaaaagaac
     5701 aacttagaag tattgctccg ttttgggaaa ataataattt acgtgctaga gctggtgcct
     5761 tattacctga agaagtgtct gtttatatgg aaacaggatt cttcggtatg gaaggtaaga
     5821 tgaattctgg agatgctcac ctagcagtta actatcagaa acttttgcaa tttggcttaa
     5881 gaggttttga agagcgggct cgtaaagcaa aagcagctct agatttaaca gatccagcaa
     5941 gtattgataa atatcatttt tacgactcta tatttatcgt aatcgatgct attaaagtat
     6001 atgcaaagcg ctttgttgct cttgctaaaa gtttagccga aaatgcaaat cctaaacgta
     6061 agaaagaatt acttgagatt gcagatattt gctctagagt cccatatgaa ccggcaacta
     6121 cttttgcaga agctattcaa tcagtttggt ttattcaatg tattttacaa attgaatcta
     6181 atggccactc tctttcatat ggccgttttg atcaatatat gtatccatat atgaaggctg
     6241 atttagaaag tggtaaagaa acagaagata gcattgttga acgtctgaca aatctttgga
     6301 ttaagacaat tacaattaat aaggttcgca gtcaatcaca tacattttct tcagcaggaa
     6361 gtcctttata tcaaaatgtt acaattggtg gacagactcg agataagaag gatgctgtta
     6421 acccattatc ttatttggta ttaaaatcag ttgcacaaac ccatctaccg caacctaatc
     6481 taactgtacg ttaccatgca ggtttagatg ctcgtttcat gaatgagtgt attgaagtga
     6541 tgaaacttgg ttttggtatg cctgcattta ataatgatga gattattatt ccttctttta
     6601 ttgcaaaagg agtattggaa gatgatgctt atgattacag tgccattgga tgtgttgaaa
     6661 cggcagttcc agggaaatgg ggctatcgtt gcacaggtat gagttatatg aacttcccta
     6721 aggttctact tatcacgatg aatgatggaa ttgatccggc ttcgggtaaa cggtttgcac
     6781 caagctttgg tcattttaag gatatgaaga acttttctga attagaaaat gcttgggata
     6841 aaacactaag atatttgaca cgaatgagtg ttattgttga aaattctatt gatttatcat
     6901 tggaacgaga agttcctgat attctatgtt cagcattgac tgatgattgt attggtcgtg
     6961 gaaaacacct taaagaaggt ggagcagtat atgattatat atcaggattg caagttggaa
     7021 ttgcaaattt gtcggattca ttagctgcaa ttaaaaaatt ggtgtttgag gaagaacgta
     7081 taagcccaag tcagctttgg catgcactgg aaacagatta tgccggagaa gaaggtaagg
     7141 tcattcaaga aatgttgatt catgatgcac ctaagtatgg taatgatgat gattatgctg
     7201 acaaattggt tactgctgct tatgacattt atgttgatga aattgctaaa tatcctaata
     7261 cacgttatgg aagaggtcca attggaggaa ttcgttattc aggaacatct tctatctcag
     7321 ccaacgtagg gcagggacgt ggaacattag caactccaga tggacgcaac gcgggtacac
     7381 cgttagcaga gggttgttca ccatcacata atatggatca acacggccct acatctgttt
     7441 taaaatctgt ttcaaaatta ccaacagatg aaatcgtagg tggggttctc ttaaatcaga
     7501 aagtaaatcc tcaaacgtta gccaaagaag aagataaatt aaaactaatt gctttgttac
     7561 gaacattctt taatcgttta catgggtacc atattcaata caatgttgtt tccagagaga
     7621 cgctgattga cgctcagaaa catcctgaaa aacacagaga cttaattgtt cgtgttgcag
     7681 gatactctgc attcttcaat gttctttcta aggcaaccca agatgacatt ataggacgta
     7741 ctgagcatac tttgtaaaat aaagaggttc tttttatgga atttatgctt gacacattaa
     7801 atttagatga gattaaaaag tggtctgaaa ttttgccgct agctggggta acttcaaatc
     7861 ccactattgc aaaaagagag ggttctatta atttttttga acgaatcaaa gatgtaagag
     7921 aattgattgg ctctacaccc tctattcatg ttcaggtgat ttctcaagat tttgaaggca
     7981 tcttaaagga tgctcataaa attcgaagac aagcaggaga tgatatattt atcaaagtac
     8041 ctgttactcc agctggatta cgtgcaataa aggcgctaaa aaaagagggc taccatatca
     8101 ctgcaacagc tatttataca gttattcagg gattattagc tatcgaagca ggagcggatt
     8161 acctagctcc atattataat agaatggaaa atctgaacat tgattcaaat tctgtcattc
     8221 gtcaattagc tcttgctatt gatagacaga actctcctag taagatttta gctgcatcct
     8281 ttaaaaatgt agcacaagta aataatgctt tagctgcagg tgcgcatgct gttacagcag
     8341 gagcggatgt ttttgaatca gctttcgcca tgccatctat ccaaaaggcg gttgatgatt
     8401 tttctgacga ttggtttgtt attcaaaata gtcgttccat ttagatagag aggaaataca
     8461 tatgagaatt tttgctagtc cttctagata tattcagggg gaaaatgcct tgtttgaaaa
     8521 tgccaaatca attttggatt tgggaaattg ccctattcta ttatgcgatc agttggttta
     8581 tgatattgtt ggaaaacgat ttgaagatta cctacatagg tatggtttcc atattgttct
     8641 ggcgctattt aatggtgaag cttctgacaa tgaaatcaat cgagttgttg ccttggctga
     8701 gaaagaaaat tgtgatagta ttatcggtct tggtggggga aagacgattg atagcgcaaa
     8761 agctattgca gatttgattg aaaagcctgt tattattgct ccaacaattg catcgaccga
     8821 cgcacctgta tctgctttat ctgttattta tacagatgaa ggtgcatttg atcattatct
     8881 attttattct aaaaatccag atttagtttt ggttgataca aaagttattt cacaagcccc
     8941 taagcgttta ttagcgtctg gtattgcaga tggtttagca acttgggttg aggcgcgtgc
     9001 ggttatgcag gcaaatggaa aaactatgtt gggacaacag caaacattgg ctggagttgc
     9061 aattgcgaag aaatgtgaag aaacgctgtt tgcagatggt ttacaggcta tggcagcttg
     9121 tgaagctaaa gtggtgacac cagcattaga aaatattgtt gaagctaata ctttattgag
     9181 tggtctaggt tttgaaagtg gaggattagc tgcggcgcat gcaattcata atggttttac
     9241 tgcattgaca ggtgacattc atcatttaac acatggtgaa aaagtagctt atggaacttt
     9301 agtacaacta ttattggaaa atagacctaa agaagaactt gataagtata ttgagtttta
     9361 caaaaaaatt ggtatgccaa caactctaaa agaaatgcat ttggatcaag ttggatatga
     9421 tgatttaata aaagttggta aacaagcaac tatggagggt gagacaattc atcagatgcc
     9481 gtttaagatt tcgccttcag atgttgctca agctattatc gctgtagatg cctatgtaaa
     9541 ttcaaaataa acaataagga ctactgtttt ccaaatggta gtcttttatt gatccctgta
     9601 ttgaattcta tagaagattg aaataggatg agaacaaatc gattgggaaa gtaaaattaa
     9661 tttctataaa tgttttagca attgtttcgt actatttcag attcagtcta ctatacgttc
     9721 ttcataaatc aaaaagcgac ataggtagtc ggctatttat tgtgagtaca ttaattagca
     9781 ttccagtttt atcttcggtc taaaataagt attttgtgct atacgagata agcttcttga
     9841 cttactcctt gatttactgc ataacaatgg gataaaaagt gggagataga gcaattcata
     9901 gtcatcaaaa ttaatgagat acagtataca gtttttcctt taaacacatt tcaaattccc
     9961 tcaaaaatgg tataatagta acatcacaaa attggagaga gaccatgagt ttttacaatc
    10021 ataaagaaat tgagcctaag tggcagggct actgggcaga acatcataca tttaagacag
    10081 gaacagatgc atcaaaacct aagttttatg cgcttgatat gttcccttat ccgtctggag
    10141 ctggtctgca cgtaggacac ccagaaggtt atactgcaac cgatatcctc agtcgttaca
    10201 aacgtgcgca aggctacaat gtccttcacc caatgggttg ggatgctttt ggtttgcctg
    10261 cagagcaata cgctatggat actggtaatg acccagcaga atttacagcg gaaaacattg
    10321 ccaacttcaa acgtcaaatc aatgcgcttg gattttctta tgactgggat cgtgaagtca
    10381 atacaacaga tccaaactac tacaagtgga ctcaatggat tttcaccaag ctttacgaaa
    10441 aaggcttggc ctatgaagct gaagtgccag taaactgggt tgaggaattg ggaactgcca
    10501 ttgccaatga agaagtgctt cctgacggaa cttctgagcg tggaggctat ccagttgtcc
    10561 gcaaaccaat gcgccaatgg atgctcaaaa tcacggctta cgcagagcgc ttgctcaatg
    10621 acttagatga actagattgg tcagagtcta tcaaggatat gcaacgcaac tggattggta
    10681 aatcaactgg tgccaatgta actttcaaag taaaaggaac agacaaggaa tttacagtct
    10741 ttactactcg tccggacaca cttttcggtg cgactttcac tgtcttggct cctgaacatg
    10801 aattagtaga cgctatcaca agttcagagc aagcagaagc tgtagcagac tataaacacc
    10861 aagccagcct taagtctgac ttggctcgta cagaccttgc taaggaaaaa actggtgttt
    10921 ggacaggtgc ctatgccatc aaccctgtca atggtaagga aatgccaatc tggattgcag
    10981 actatgtcct tgctagttat ggaacaggtg cggttatggc tgtgcctgcc cacgaccaac
    11041 gtgactggga atttgccaaa caatttgacc ttccaatcgt cgaagtactt gaaggtggag
    11101 atgtcaaaga agctgcctac acagaggatg gcctgcatgt caattcagac ttcctagatg
    11161 aactcaacaa agaagacgct attgccaaga ttgtggcttg gttggaagaa aaaggctgtg
    11221 gtcaggagaa ggttacctac cgtctccgcg actggctctt tagccgtcaa cgttactggg
    11281 gtgagccaat tccaatcatt cattgggaag atggaacttc aacagctgtt cctgaaactg
    11341 aattgccgct tgtcttgcct gtaaccaagg atatccgtcc ttcaggtact ggtgaaagtc
    11401 cactagctaa cttgacagat tggcttgaag tgactcgtga agatggtgtc aaaggtcgtc
    11461 gtgaaaccaa cactatgcca caatgggctg gttcaagctg gtactacctc cgctatattg
    11521 atccacacaa tactgagaaa ttggctgatg aggacctcct caaacaatgg ttgccagtag
    11581 atatctacgt gggtggtgcg gaacatgctg tacttcactt gctttacgct cgtttctggc
    11641 acaaattcct ctatgacctc ggtgttgttc cgactaagga accattccaa aaactcttta
    11701 atcaagggat gattttggga acaagctacc gtgaccaccg tggtgctctt gtggcaaccg
    11761 acaaggttga aaaacgtgat ggttccttct ttcatgtaga aacaggggaa gagttggagc
    11821 aagcgccagc caagatgtct aaatcgctca agaacgttgt taacccagac gatgtggtgg
    11881 aacaatacgg tgccgatacc cttcgtgttt atgaaatgtt tatgggacca ctcgatgctt
    11941 cgattgcttg gtcagaagaa ggtttggaag gaagccgtaa gttccttgac cgagtttacc
    12001 gtttgattac aagtaaagaa atccttgcgg aaaacaatgg tgctcttgac aaggtttaca
    12061 acgaaactgt taaagctgtc acagagcaaa ttgattccct caaattcaac acagccattg
    12121 cccaacttat ggtctttgtc aatgcggcta acaaggaaga caagctctat gttgactatg
    12181 ccaaaggatt tatccaattg attgccccat ttgcacctca cttggcagaa gaactctggc
    12241 aaactgttgc tgcaacaggt gagtcaatct cttacgtgac ttggccaaca tgggacgaaa
    12301 gtaaactggt tgaagacgaa atcgaaatcg ttgtccaaat caaaggtaaa gtccgtgcca
    12361 aactcatggt cgctaaagac ctatcacgtg aagaattgca agaaattgct ctagcggatg
    12421 aaaaagtcaa agcagaaatt gacggtaagg aaatcgtgaa agtgattagt gtaccaaata
    12481 aattggttaa tatcgtcgtt aaataacgag tttattagct ctatctgcca ccttcaatag
    12541 tccactggac tattgaagcc aactaaatta gttaacattg ttgtgaaata aaaagaagta
    12601 actctagagt atgaacttta gagttacttt tagaataggt cagaatgaat agattagtga
    12661 acgatagcag gaagtgtgaa attttattat ttaagatttc tattattgta gtgtcagtta
    12721 tagtattagt aatgtcaata gtgggtgcat attattttaa aaattgtctt tgggacaagg
    12781 aaagtttaaa tttgacactc actttagaca ctctctttgc agtttttatt actttcttta
    12841 gtttttcaaa tgataaatca agcaatattt ttggaatatc tccaaatgaa attataagga
    12901 cacagtctga agtgtatcaa tttttattta caaaatcttt tgcaactcta ttaatagtga
    12961 caaatttgtt atccattatt cattattttg gagccaaaaa tttaatagtt tcttttaatg
    13021 aaacttatcc tcgtattcaa tccagtgttg gtatcatgtg gctaccactg tttggtattc
    13081 tgataatcta tt
//
LOCUS       DAVKLX010000056        12882 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKLX010000056 DAVKLX010000000
VERSION     DAVKLX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.056"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(389..2116)
                     /gene="ezrA"
                     /locus_tag="VW325_001802"
     CDS             complement(389..2116)
                     /gene="ezrA"
                     /locus_tag="VW325_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9735145.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIDEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2198..4144)
                     /gene="gyrB"
                     /locus_tag="VW325_001803"
     CDS             complement(2198..4144)
                     /gene="gyrB"
                     /locus_tag="VW325_001803"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9735146.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4159..4722)
                     /locus_tag="VW325_001804"
     CDS             complement(4159..4722)
                     /locus_tag="VW325_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9735147.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4807..5361)
                     /locus_tag="VW325_001805"
     CDS             complement(4807..5361)
                     /locus_tag="VW325_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9735148.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5372..6595)
                     /locus_tag="VW325_001806"
     CDS             complement(5372..6595)
                     /locus_tag="VW325_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9735149.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6632..9082)
                     /locus_tag="VW325_001807"
     CDS             complement(6632..9082)
                     /locus_tag="VW325_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9735150.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLRKKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTEASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEDFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVGHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELSQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9282..10211)
                     /locus_tag="VW325_001808"
     CDS             complement(9282..10211)
                     /locus_tag="VW325_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9735151.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     AQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10351..10638
                     /locus_tag="VW325_001809"
     CDS             10351..10638
                     /locus_tag="VW325_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9735152.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10672..12006)
                     /gene="ciaH"
                     /locus_tag="VW325_001810"
     CDS             complement(10672..12006)
                     /gene="ciaH"
                     /locus_tag="VW325_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9735153.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11996..12670)
                     /gene="ciaR"
                     /locus_tag="VW325_001811"
     CDS             complement(11996..12670)
                     /gene="ciaR"
                     /locus_tag="VW325_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9735154.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
BASE COUNT         3921 a         2745 c         2293 g         3923 t
ORIGIN      
        1 agaaaagtgc aaataagaaa tctccagatt aggaactatc cgtgagttct ctagtctgga
       61 gatttttcaa tagacttcgt tattggacgg ttaccaagtt actcaaagta cagatttgaa
      121 gctgcagata agactgacga aatcacttta atactaaatg aaaatcaaaa agcaaactaa
      181 gaagctagcc tcaggctgta cttgagtacg gtaaggcgac gctgacgtga tttgaagaga
      241 ttttcgaaga gtataaaaca ctgttttgag gtggtggata aatcggctgg gtaaaaactc
      301 aatctcagga gaaaatgaag taaatctccc cacaactata gaaaaagatt tgattctgct
      361 cctcacacaa taaaatcttt ttcttttatt aaaaacgaat cgtttcacgt gttttctcat
      421 atgaggtaac aaagcgattg gttacaccag gctctgccac ttccaatgct tgagaaatct
      481 tgtcaaatga agcgtgataa tcaaattctt tttcaaaaat atctaaagct tcgttaaatg
      541 cttcttgaat gcgttcatca aatgagcgat agcggttaga atattgcaag agttgctctg
      601 tcaaagttgc atattgtaca atattataag tttccgtttc taaagcttcc atatcattcg
      661 ttgcaatttc aagaactcgg gtaacagatt caatgttaat cattttttgt tctaactcaa
      721 ccattaaatc ctcggtatta ttgcttgccg taaagaataa cttcaagaaa gtttgtggaa
      781 tacctggcag attgcgtttt tccatgtatc gcttgatagt atggagacga ttgacataaa
      841 cattggcctt ttgacgtgca ttaatatcat ctttctcaat ttgtgtcagg cgctcactaa
      901 ctgaaatttg ctcatcttca atatctttta gttgagtttg taaatcctca agattttctt
      961 caagaactga ataagcttgg gttggttctt cttgatttga agttacctca acaatagctg
     1021 cctcaaaact ctctaattct gtctgaatac gacgaacatg gcttgcagct gtctcaggaa
     1081 gtaaataggt cttgttcaaa cgtgcaatat cttctcccaa taaagtatta ttctctttca
     1141 tatgttgaag gtaagttgga agagttgcaa gtagattttc cactactttc tgagcagcaa
     1201 tttctcgagt aaaaatatca tacaaggcat tgatttcctc ttgtgcctgt ccattctcat
     1261 attcggcatt atccaattcc aactgacgaa tattctcttg gtttttcttg aatgcttcat
     1321 aaagcaagtg gaaacgcgct tcaatatccg tttcaacaaa atgataatta gcatcaatta
     1381 gtttacgata accggattcc aaatcctgta attgatctgg caattctgta gaaagcgtcg
     1441 taaccaaggc tggaacacga tccacaatat gacttaaggc caaaatgtga ttttctgtat
     1501 tatccaaaat cactgcggct tccacagggt cacccgatga attcaaggtt acaaattgtg
     1561 aaaattcaga ttggatattt tctaattgtt tttcaatttc atccaaggct tgaccatact
     1621 gttctgaatt ttcagcaact ctatgctgaa gttcctcaaa taaatccaaa gcatgaagaa
     1681 cacgaccact atttttagat tcttgcttct ctaagtctgc caaagcattg cgaattgccg
     1741 caatatcttc gtcaatcaaa gtaatttgac tctcaatttg gtcaatttga tgactggcct
     1801 tgagaaaacg aaatgaatgg ttatagcctt ctgcttcaaa gagattattt tcaatatcgg
     1861 caaaagagtt gagagataaa tcgacccatt tttgattcca ttcacggaaa gccacttgac
     1921 tttgtccaat caagtgcata ttttttacag cttctacttc atcatttact ggaagattgt
     1981 atagttcttc ttttctttct tctagcgcct ctaatctccc ctcgtttcgc ttacgtagaa
     2041 agattgccac tacataagcc agaactaaaa tgactgcaat tgcaaccatt aaataaatta
     2101 gttgtccatt agacatatca aactcctttt ttacttgaaa caatcgtaac gattatatca
     2161 tattttttag accaagggaa ctacttctcc ctatttttta gacatcaagt gtactataga
     2221 cagcattttc ttcgataaac tcacgacgag gctctactcg atcccccatc aacatatcaa
     2281 agattttatc tgcttctgca gcatcatcta cagaaactct agccatcaag cgatgttcgg
     2341 gatccatggt tgtttcccac agctgatgat cgtccatttc acctagcccc ttataacgct
     2401 gaatagtcgg tttggtacga ccttcactat aacgggctaa agcttcttgg agtttgattt
     2461 cttgatctgc acccggctgg atatattctt taatctcgct tccaaccttg acaccataga
     2521 ttggtggttg ggcaatataa acataaccag cttctaggat tggtttcata taacgataaa
     2581 tcaaggttaa aagaagggta cgaatgtggg ctccatcgac atcggcatcg gtcatcaaaa
     2641 cgagtttttg gtaacgggct ttcgaaacat caaattctgc gccaaatcct gttcccatgg
     2701 ctgtgaaaag actacgaatt tcttcgttag ctagaatctt atccatactt gctttttcaa
     2761 cgttcaaaat cttaccgcga attggaagga tagcctgaaa ctcacggtta cgaccagatt
     2821 tggctgatcc accagctgag tctccttcga cgatgaagag ttctgtttca gcagggttat
     2881 tagaagaaca gtctgctagt ttccctggaa ggttggaaat ttccaaacca gattttttac
     2941 gtgtgacttc acgcgcacgc ttggcagcca cacgagcctt ggcagctaaa atcccttttt
     3001 ccacgatacg tttggcaatc tgtggatttt ccatgaggaa atcggagaag gcttcactga
     3061 agaggcgatt ggtaatcttg accacttcgc tatttcccaa tttggtcttg gtttgtcctt
     3121 caaactgtgg atttgggtgt ttaactgaga taactgcagt taatccttcg cgaacatctt
     3181 cccctgttaa attatcttca ttgtctttca gtaacttatt cttacgagca taatcattga
     3241 taacacgagt cagtgccgta cggaaacctt gttcatgcgt tccaccttca tgggtatgaa
     3301 tattattggc gaaactcatg acattttcat ggtaacctgt tgtgtactgc atggctacct
     3361 caactgtgat atcatccatc tcaccgtctg tatagattgg tgtatcaaag attacatcct
     3421 tgttctcgtt gatatattca acgtaactag caatcccacc ttcataatga taatgcttgg
     3481 tttgttccaa accttggcgc ttatctgtaa ttgaaatttg aagaccgcga tttagaaagg
     3541 ccaactcttg aatccgttta tttaatttat caaaatcaaa gattgttgtt tcagtgaaaa
     3601 tttctgggtc cggtgtgaag tgaaccgttg ttccagttct atctgtatcc ccaaccacct
     3661 caaggtctgc aacaacatga ccacgacggt attcttggta atgaatctta ccgtttttat
     3721 ggacatgaac gtctaattga gtggaaaggg cattaactac tgacgacccc accccgtgaa
     3781 gaccacctga aaccttgtat ccaccaccgc cgaactttcc tccagcatga aggactgtaa
     3841 agacggtctc aacagcagga cggcctgttt tttcctgaat atcgactggg ataccacgcc
     3901 catcatccac aacagtaatc gaatcatctg gctcaataaa aacttgaata tggctggcaa
     3961 atcctgccaa ggcctcgtca attgagttat caacaatttc ccagactaga tggtgaagac
     4021 cttcttttga ggttgatcca atgtacatcc ctggacgcat acgaacagcc tctaagccct
     4081 ctaaaacttg aatttgactg gcatcataat cctgtgcctg cagatttttg atttcttctg
     4141 tcatcttatt cctttttctt atatgtctaa tacttgtctt aaattcacga tactggtaaa
     4201 caagtgggta tcaagtcctg cagcttgacc tgcttcgata tcaatcggcc gatcaccaat
     4261 gacaagacca gagctaatct gatacttttc tcttaaataa agcatggatt cgggatttgg
     4321 ctttctctta aagcctgagc tagaagtcac cacttctgta aaataagctg ctatagaggt
     4381 tttttctaaa atttccaaaa cctgatcatt tcgatgagag accaaaaaat gacggccacc
     4441 ttgatttaaa atgtcttcca ataggtcaga aactccttca aataaaatcg ggtgttcaag
     4501 ctctctggct tcattttcct tgtacttttc taaaaaattc tctaaattgg gagcgaatgt
     4561 ctcaatcgca aaaggagtag aaacctttaa agcttgatag acactgtcat ggtcttgtgt
     4621 gataccatac agtgccaatg tttcaacaaa tgcagctgtt gaagtttcat aattatccag
     4681 taaagttcca cctaaatccc agatgtaatc gtgatatttc atacccttca ttatagcatt
     4741 tttttatgaa aaaagcgatg atttccctga gttttccaca taaaaaacga gagaataact
     4801 cccgttttac tgatttttac caaagacatc tcgatagagc attcctgttc gtaaactctc
     4861 tgcgtcccct cctagttgct ccaccaactc gtaggcaaag gcaagggctg ttgaaggacc
     4921 ccgactggtt gtcaactgac catctaccac tactgtttcc ttgacgtagt gaccatcaag
     4981 gatttgctct tgaacgccgt cataacaagt gtatcgctta tttttcaata tctctgcttg
     5041 attgagggca attggtgccg cacaaatggc tgctagtttc ttcccttctt gctcgaagct
     5101 ttgcaattct tgaatcaagg tctgattatc acgtaaatgt gcagaaccag gcatacctcc
     5161 aggaagaaca atcatatcat agtctgataa atctccatca aagacatgat ctgctcttac
     5221 ttggattgca tgcgaacccg ttacttgctc ttcaaaacca accatatcac atgtgatatt
     5281 ggctcgacgc aagacatcta caactgtcaa ggcttcaatt tcttcaaagc cctgagctaa
     5341 cataactgct acttttacca tgattttctc cttatttaat ttgttttaat acaacctttt
     5401 tccgtttgaa tgggactttt ccgctctttt cttcagctaa attagtctgg taactcatcg
     5461 ataaaagcaa accaacacca atcaaattac tgataatagc tgatccccct tgcgaaatga
     5521 aaggcaaggg aatccccgtc aaaggaagta gtccagtcac agcaccgata ttctcaaaga
     5581 tgtggaagag caacatcata atcaaacctg tggaaatata agtgtagaac tggttatttg
     5641 atttaagagt aatcttcaac atacggtaaa tcaacatgag atagagggca ataaccagga
     5701 cagagccaat aaagccaaaa tcttctgcaa taaccgtaaa aatcatatct gactctcgaa
     5761 ctgggataag cagattcgaa gcattaaatc cctgaccaaa taagccacca ctcccaatgg
     5821 caatctgccc ttgagcctgc tggtaagtcg ttgtttgggc aaactcaaag ggattgagcc
     5881 aagccaaaat ccgattaatt tggtaagtcg gcattccaat ctggtgaaga aaagctcgtc
     5941 cgtccttgct aataaagata gctaagaaac cagcaactcc tgttacagca gtcacaaata
     6001 ctgggataat aattttccaa gaaacccctg ataataaaac gattcctgag aaaatggcta
     6061 caaaaaccaa agccgtcccc aagtcacttt gaagtgctaa aagaactagg actggaatgg
     6121 taaagagaat catccagaaa attaacaaaa agtccagcgg aaccgtgcgt ctccattcct
     6181 tatgtttctt tgtaaattgg acaatgacac gagccaacat gaggatatag gatatcttca
     6241 taaattctga cggttgaaat agggtaattc catttattga tacccagttt ttggcacccg
     6301 ttgatgcaac taagcttgga ttataaaata caatcggcaa gatcataagt cccaagccta
     6361 aaatatatag aaagggggtc accttccaaa gaaattctgt attaaagagc atgaccacaa
     6421 aaccaatcac aagccccaag gcaatccagg cgacctgctg ccctaaaatg ggcagaatat
     6481 tattgggata atcatgacta acggctatat agatagccac cacaccgatg accagtagaa
     6541 aaaatactgg caagagcaaa ctataatcga ctctagagtc gagagaacgt ttcatatgta
     6601 ctaaccttat actttcatac aatactattt atcaaagttc attaaaaaat ctatcaatag
     6661 cctcgtcaac ttcggatcga gagatggttt taacagtcgc ttcttctgct agagatgcta
     6721 ctatttgttt gccgtatcgt tttccgatga ttctcctatc caaaataaga gttaaggaac
     6781 gttggtattc acgtctcata cttcttccca aagcctgttt taaacgaata atggccattg
     6841 gcaattgata atcataaaag gcatttttcc cttcttgact cagttcttga ttaatctttt
     6901 tcgtcaaggg ttcttgagga ttttggaaag gaagcctcgg tacaacttga atcacaaaag
     6961 gatggcttga aaaatcaact ccctcccaga aacttgctgc accaagcaag atttgttgtt
     7021 cacctttttc aaagcgtttc tttagctgat gaacatcccc atttttatac tgggccaagt
     7081 ggccaactgt aagtaaatcc gatactgcta gaagcatgtc tttagcggta aagagaacca
     7141 aaatcggttg ctggaaagct tgaatttcca ctagtaaagc agctacctct ctggcataga
     7201 cttctaagga ggtttctgtt accagaggga aatctttgac catgaccact tcttgttcct
     7261 gttttccccg agattcaatc ttgacaaatt tagcttcagg atagcctaaa aggtctgcca
     7321 aagaaaccct ctgactaatc tcaagagtag ccgatactcc caagacttgg caactctcag
     7381 gaagtaaaga ggacagaaga gtacggcctg attttgtaga agaaatctga attttctttt
     7441 gactcgtttc agttacttca agccagtaat caccttcagc tgtaaaatag cgaaccagct
     7501 catcaaagtc ttctacttcc aattctgaaa aatactgatg gagattgtcc agagaatcta
     7561 agatattttt cctagatttg ccagactgaa attgttctat caagtagaga cactcaaagc
     7621 gaatactttc tagtatccgt tgttgaaccc tgttttcttc tcctactaaa gccttatcaa
     7681 ttaaatcgat aatagattgt atatcgtagg tctcttgaag cagattttct agagctaaca
     7741 aaatcttttg gacttcatca ataatcagta aacggtcact gacaaattca ggattatctt
     7801 caagtctggt tacgagatag gcatgattag tcactaaaag cttgcaagtc tctgcccttt
     7861 cttgactacg tttccaaaaa tcttccgtca caaataagct ctgggatgat aaattcccat
     7921 catgacgaag gtctgctaga aaatgttggt aacggtagag ttgcccgatt tcatccaaat
     7981 ctcctgtctc tgtctcagta agccagacca agacttgcat tttaaagcgt ctaaataagc
     8041 gattttcatc attttcctgc aaggaatgat aaaaggcatc caacttcaga taattttgtg
     8101 gtccctttaa gctatgaata tctgtatgga acacttcctt gaggcgttta ccttcttctt
     8161 ccatgatttg attttgaaga atctttgtcg gaacactaag aacaatttgt cgctctttgg
     8221 attgagataa agcgggtaag agatagccat aggttttccc aatccctgtc ggcgcttgaa
     8281 tcagagagac aggttcatct ttcaatagca agccaacctc tttagcaaaa ctttcttgtt
     8341 cctccctcac ttcaaggttc aacagagaaa tatttttaga aaagtcttga gatagttttc
     8401 gtggctccag agaagcttcc gttttcttaa aatatagacc ttgaacttgg accaagtctg
     8461 gagaactcag gatagattgg ttgcgataag tttcctcaat aaccaggtag gactcatata
     8521 ggagagcgtc agccatttcc agcaagcgtt ccaagagacc tttaggaagc tgggtcatct
     8581 ttttccgtaa aaaaagaagt aattctgctg tagcttgggc atctgaaagg gctgtgtgtg
     8641 cgtgtttaag aggaattcct aattctcgac acaaaatcgg caaactatat ttttccagtt
     8701 cagggaaaaa gacctgggcc aattcgaccg tatcaacacg agggtttctt agctcatagc
     8761 cttcaaaaaa taaattttcc gccaagagat tagcatcaaa ctgaacatta tgggctacaa
     8821 aaatcccatc ctccaccaag tcaaatattt ttctggcaac ttgcgaaaaa tcaggtgctt
     8881 gcgccagacg ttggtctgtc aatcctgtca gttctttgat atgagcatcc aagggttcat
     8941 gtggattgac atccgtcgta tagtgatcga cgatttctcc gtcctcaatc acgacaattc
     9001 ccacttggat aattttagcc ttactacctg tgctagttgc ctctaaatcc accacaacat
     9061 agcgattacc aatcgttttc attataaaaa attataccaa aaaaagggcc atttggcacc
     9121 tgcaaacata ggataatttt caaactcaag aacactactt ttcttaaaaa tcccatcgaa
     9181 tagagcattc tagcctgctg atttttgtaa aatagtgaag aaatacgaaa aattgagaga
     9241 agaaatgttt gcatcttcct tctctcaacg aatcatgtat tttatctaac catgtcagga
     9301 tcgtagtcat aaaatcctgt atcaaggaaa cggtttttag ggtgaagttt acgaacttct
     9361 tcatccagca agaaggcttg agcatgacta aagttgccct cttgaatcaa gctacgcgct
     9421 tggataaaga gttttgcaat ctcaacaaag ttctgaccag gagtgtagag cccttctagt
     9481 ttccagcgag taaagccatg ctccaccaat tctgttaatt tgatcatcaa atcaaggtca
     9541 ttgttggcaa agatatgggt cccatgatta tcttcaaaaa tggaatagtg gctctctgga
     9601 tcacttggct cagccaagaa gaggtcacgt ttatgcgtct tttcatcatc gatatgtgta
     9661 aagttatagt agttttgcaa gagtggacgt ttagaatgat ggatgacgct agcaccgtaa
     9721 accaaaactt cagcaggaat ttccaaaatc tctggcattt tgaaaagttc agctgatgga
     9781 atttcacgcg ccaaaacagc ctcagatgcg ccagcctttt gtccccagaa gttaatctga
     9841 cgactgctag ttaccatggt tgaagcatcg tagatggtct taaatgaata accatcgcgg
     9901 ttaactacgt aaaagacgcc tgcatcccca atcgtaatat agtctgtctt gatttcttcc
     9961 aagaagtcta agaaaggctt gatacggtcc atcatatctt ggtgcatgag agcattgacc
    10021 gcaacgatca attccttacc agcatcatga accaacttag cgatttcacg taattggtca
    10081 taactaaagg tcgttggcag acgaagacca aaatctttct caccgacata gatacggtct
    10141 acgccagctt cgagtagttg ttcaacttgt tcaatacttt cagcagttgc tgtaatgaca
    10201 atcttttcca taagaaaaat tataccacat ttctccaaaa tcagccatgc tttgaaaatg
    10261 aaaagggact ttattctttt tgtaaccttt ctgtaataat tctctgccga aaaaatgata
    10321 aaataggtat gtactgttaa ggagagaatc atgcccgtaa gaaaattaca atcctatgag
    10381 gtagactatc aagaagaatt aaaccagcag cttcctcatt accaagctta tacacctgaa
    10441 gcgcaatctg atgccaatct caaggaaatc ctatttttta ttaatatcgc tgttttttgt
    10501 atctgtattg ctatctttag ttttatcttt ttagcattaa aattatcaac tgctcttgcc
    10561 tttgccgcag caatcggatt cagcttactt gttttaaaag ttcaacggtc tattatcaaa
    10621 cgaaaacgta gaagataaat ccaaaatatc gccccaattg gagcgatatt tttatttttt
    10681 ctttttagat ggtgtctgaa tggcaatctt cacttcaaag attgttccct tgggtttatt
    10741 atctttgaca gtaacagttc cttttagagc atctacaatt tgcttggcta gggataatcc
    10801 taaaccaaaa ccaccttttt gccgggttct agccttgtct actcgataaa aacggtcaaa
    10861 aattttcttt ttatcttctg tcgaaatacc gattccatta tcagaaacaa gtaaataaag
    10921 attgcgatcg gtcgccgaga taagaaaatc aatttcacca tcctcctcag tatacttgac
    10981 ggcattatcg aaaagaatgg tcatcagttg tttcagaaga agctgatctg tgacaattgt
    11041 tcgatggata cgattttcaa aacggaagac acgattattt tccgaagcaa tcatctcata
    11101 gtttgtgaaa gttgtattaa aaaagctagt tggaacttct gcaagctccg gcttaatccc
    11161 atcatctctc cgagctaagt tcagcaagct tgtcgttaaa aaacgcatat ttcggacttc
    11221 ttccaaactc gatgcaatgc tttcgctcac atccataatg gtagcttctg gcttacgaaa
    11281 aagggtctct aagcgatttt gcaaaactgc gagtggagtt cgtaactcat gactggcatt
    11341 ttccacaaaa gactgttgct tctgcatact ctcaagcagg ggcctaacac tgaccctagc
    11401 tagatagaga ctggcaagta aagacaaaat ccagaaacta gccatcacga ccacaatcaa
    11461 ttgctcatgc ttttgactag cctgctccaa ctgactggta ttaatcaaga cagcagcata
    11521 cttgatattg gttgaaaccg aactaatatt ggtctccatc aaaatcacac gatagatttc
    11581 ttcctgccca tagctattaa aaacctgaat ctggtagata tgtcctagtt ctttcttttc
    11641 taacttaatc ttatccaagc ccaaaaatcg atttccagaa agaagttgag taaagtcttt
    11701 atcaaagaga atgacttccg tattggaact gacattaggt tttatttcta ctttactagc
    11761 gtccgcccta gcattttcta aatctttaat ctcttctgtt gccctattta tagccagctg
    11821 aataactgct tgaggatttt cactcagtcc atgaagctta tcgtccaccg aagtataaag
    11881 actcgaatgc atgacttgta aaataatcag agtcattgta gaaaaaatca gggtgaagac
    11941 accgaagttg cggataaaat aactaaagtc atccgcatac catgtttttt taagtttact
    12001 gaacatcttt taaaagatac ccaacactac gcaaggtttg caaattctct gcaaaagtgg
    12061 ttccctttaa tttcttacgg acttttgaaa catagacttc gacaaccgaa atcgttgtat
    12121 cactatcaaa tccccataga cggtcaaaaa tctgcgtctt aggcaaaatc acattttgat
    12181 tttgaaggaa ataaactagt aaatcgaact ctttccccag caattcgaca ggagtatctt
    12241 caactttaac ggtattggtt gataaattaa ccacgatatt cccataagtc aaggtgtttt
    12301 cattaaactt ccctgaacgt ttgagaaggg cctgaattcg cattttaagt tcttctaggt
    12361 agaaaggttt ggtcagataa tcatccgctc ccagttcaaa tccatgtccc ttgtcatcca
    12421 aactttcctt ggcagtcata atcagaactg gtgtcgtaat tcccttttca cgcaattctt
    12481 ttaagacttg gaaaccattt ttttctggca acatcaaatc cagcaaaatc aagtcataga
    12541 caccactctc agcttcgtag agaccttctt ctccatcaaa tacctgcata acatccgcaa
    12601 aatcgtctaa aaagtcaaat actgaatttg acagacctag gtcatcctca accaataaga
    12661 tttttatcat gagaaactcc tccttattaa aactattata ccaaatttgc cttaaaaaaa
    12721 actcaactct ctgcatttta catgagatag ctgagttttc tttggctcta taatatttgt
    12781 agtgggtaaa tcccctatgg atattatgga gcttattttt gtgtagaaaa aaagtcccat
    12841 atgacctata atgaaaagcg acaaaacaac tcattagaaa ga
//
LOCUS       DAVKLX010000057        12863 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKLX010000057 DAVKLX010000000
VERSION     DAVKLX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12863
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.057"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            122..277
                     /locus_tag="VW325_001812"
                     /pseudo
     CDS             122..277
                     /locus_tag="VW325_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            477..3752
                     /gene="rexB"
                     /locus_tag="VW325_001813"
     CDS             477..3752
                     /gene="rexB"
                     /locus_tag="VW325_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9735155.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGAEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYIDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERKNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQPITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3749..7399
                     /gene="addA"
                     /locus_tag="VW325_001814"
     CDS             3749..7399
                     /gene="addA"
                     /locus_tag="VW325_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9735156.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGCQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQITILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7614..8792
                     /locus_tag="VW325_001815"
     CDS             7614..8792
                     /locus_tag="VW325_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735157.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            9001..>12863
                     /locus_tag="VW325_001816"
     CDS             9001..>12863
                     /locus_tag="VW325_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9735158.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTK
                     KAQVIKEQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKG
                     VPEVQPALPEAVMTDKGDPEQVEPLPEYTGVQAGAIVEPEKVEPEYAGVQAGAIVEPE
                     QVAPLPEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVE
                     PEKVEPSKEYTGVQAGAIVEPEQIAPLPEYTGVQAGAIVEPEKVEALKEYTGKIEQPS
                     AEDTKPNNENTNTPEEMSIQKKSSALINMNFITDSSKVTGVGSATFIAPNVLLTVAHN
                     FINNSTDNTTGEFRGDKSKNVYEWVTPDGQKGTFTANNIHFYNKKDYPKGFIYDLAVI
                     KLPETTGREHVELVKNYSKVNLNDKLNVHGYPAGKYTHLKDATVEMEQEYANNTYGVQ
                     YQGGNPGMSGGGIFNANGEVIGVHQNGAQNRSGGLILSPTQLAWIKSIIAGNEIPPVY
                     DELYRHKDEKKDDAKDEKEVIKKLELRNISSVELYSKDGNKYRHVTSLASLPSNAENY
                     FMKVKSENFKDVMLPVTSITNDTKDNRDVYKIVASANSLIQHENNNVLENYTYYLPKT
                     QQSETGVYTSFKNLVDAMNSNPNGTFRLGATMDAREVELPDGQESYVNNVFHGILVGT
                     NNEKYYAIYNLKKPLFGELNGATVEKLSLKDVNISAKDDTATLAKEANNNTHIDNVHA
                     DGAIAGERSIGGLVSQVNNSTISNSSYTGRITNTYKTVASYQIGGLVGKLSGPRGLID
                     KSFASIDLSSNATQGDQSIGGIVGAVENSALISNSYAEGNLNNVQRFANVGGVVGNLW
                     DPVGGLEKSGRLSNVLSDVNVTNGNAIAGYNFNGIKANGTYSNKNNKVVNVVQEDDEI
                     LTKDSTVQRGEVLEDAQIKEKKATFV"
BASE COUNT         4264 a         2454 c         2703 g         3442 t
ORIGIN      
        1 tagattgaaa taagatacga acagcttgat tagggaaatc aaattaattt ctagaaatat
       61 tttagcatcc acggtgtact attatagttt caatttacta tagttcaatc attctatcaa
      121 gaatattgaa tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc
      181 cgatgttgat ggcgataaat cagacaatct acccgtcgac attaagatac tgggacaaca
      241 cattgaagtg tattccgtac ctaagaaaca atcataaaac tcaaagcctt gttaccgagg
      301 cttttttatt atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa
      361 gtatttaaga tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat
      421 ttacgaggct aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga
      481 aattacttta tactgatatt cgaacttctt tgacagaaat tctaacaaga gaggcagaag
      541 agctagttgc agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa
      601 aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc
      661 gctttgctca aatggctcgt tatctggtct tgaatgattt accagctaaa actactcttg
      721 atgatatcgg tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact
      781 tgcgtgttta tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt
      841 accatgagat gaccaaatct cagatgaatt ttttggactt ggagaattta acagatgagg
      901 ataagagggc ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaagggc
      961 agttagccca gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa
     1021 gtagtgattt taatcaaatc gccttggtca ttgacggctt tactcgtttt tctgctgagg
     1081 aagagcgggt tgtggactta cttcacggca aaggtgctga gattgttatc ggggcttatg
     1141 ctagtaagaa agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa
     1201 aatttctcca tcatctggct tctaaatacc aaacgcccgc tcaggactgt tctcaaactc
     1261 atgagaagat ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt
     1321 cagaactcgc tttggatgtc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt
     1381 tgacgcaaaa ggaggagttg gagctagtag cccgtagcat tcgtcagaaa ttacatgaga
     1441 actcagacct gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt
     1501 tatctctcaa aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag
     1561 ccatggctca tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc
     1621 gttttcgtca ggaggatttg attaatcttc ttagaactga cttgtatatt gacctcagtc
     1681 agtctgatat tgatgctttt gaacaatata tccgctatct tggtattaat ggcttgccag
     1741 cctttcagca aaccttcacc aaatcccacc atggaaaatt taatcttgag cgtttgaatg
     1801 tcctccgcct gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg
     1861 ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt
     1921 tacaagattt gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga
     1981 aggctttttg ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc
     2041 tggaagactt cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc
     2101 cagcaacagt ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg
     2161 actttgtcta tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca
     2221 gtcttctgac agatgaagaa aggaaaaacc taaaccaaac gaccgaagaa ggcgttcaat
     2281 tactgattgc cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca
     2341 attctgctcg taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta
     2401 aggaatctgc ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagaa
     2461 tgaatcacaa aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc
     2521 tggttgccta tcaccagcag ggtgagatga gcgatactga gcaagatttg acttttgtca
     2581 aggttctgtc gcgtgtcata ggtaaaaaac tagatctgca aggtctggaa aatccagcta
     2641 tcccaaccag tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg
     2701 ccaaacagga gttttacctg tctacgtcgg gtttgacaga gttttatcgc aatgaataca
     2761 gttatttcct acgctacgtt ttaggcttgc aggaggaatt acgtttgcgt cctgatgccc
     2821 gtagtcacgg gaatttcttg catcgtatct ttgaacgcgc cttacagttg cctaatgaag
     2881 attcctttga ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg
     2941 aagctattta tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg
     3001 cacggacgac tggacatatt ctccgacaca atccagccat cgaaaccatc aaagaagaag
     3061 caaattttgg tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg
     3121 tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag
     3181 actacaaatc cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc
     3241 agttaccaac ctatcttgct gccctaaaaa gagaagggga gcagaacttt ttcggcgcca
     3301 tgtacttgga aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag
     3361 cagtggtaga agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca
     3421 gttatttagg cgaattttat aacaaaaaca aggctaatca actgacagat gaggaatttc
     3481 agctcctact ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag
     3541 gccggttcgc catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc
     3601 aacatcagcc tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag
     3661 aaaagttgga cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggt
     3721 ttgaaaaaat aagagaggag ttaaatcgat gaagcttatt ccctttttaa gtgaggagga
     3781 gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag gaacagaaga aaactgccga
     3841 acaaatcgaa gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc
     3901 tggaaagacc tttgtcatgg tagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat
     3961 ttctcaactc tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt
     4021 agagaaaaaa atcagcaaga aaatccaaga aacagatgat gtcgatctca aacaacactt
     4081 gggttgccag ttggcagacc tacccaacgc tgccatcgga accatggact ctttcacaca
     4141 aaaattcctt ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca
     4201 aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca
     4261 ttaccaaggt aaacagaaag agagctttag tcatttgctg aaaaactttg ctgggcgtgg
     4321 caaggacgaa cggggtctgc gccagcaagt ctataaaatc tatgacttcc tccaatccac
     4381 cagtaatcct caaaagtggc tgagtgaatc tttcctcaaa ggctttgaga aagctgattt
     4441 taccagtgaa aaagaaaaac tgactgagca aatcaaacaa gccctctggg atttggaaag
     4501 ctttttccgt taccatctgg ataacgatgc caaggagttt gcaaaggctg cctatttaga
     4561 aaatgttcag ttaattctgg atgaaattgg ctccctaaat caggagttcg atagtcaggc
     4621 ttatcaggca gtgcttgcgc gtgttgtcgc catctctaag gagaaaaacg gtcgagctct
     4681 gactaatgcc agccgtaagg ctgatttgaa gcccctggct gatgcctaca acgaagagag
     4741 aaagacccag tttactaaac taggacaatt atcagaccag ataaccattc tcgactatca
     4801 agaacgttat catggagaca cttggaaact agctaaaacc ttccaatctt tcatgagcga
     4861 ttttgtagag gcttatcgtc agagaaaacg acaggaaaat gcctttgaat tcgctgatat
     4921 cagccattac accattgaga ttttagaaaa tttcccacaa gttcgtgagt cttatcagga
     4981 gcgcttccat gaagtcatgg tcgatgagta tcaggatacc aaccatattc aagaacggat
     5041 gctggaattg ttgtctaatg gccacaatcg ctttatggtg ggagatatca agcaatccat
     5101 ctatcgtttc cgtcaggcag acccgcagat tttcaatgag aaattccaac gctatgcgca
     5161 aaatcctcaa gaaggcaagc tcattctcct caaggaaaat ttccgtagta gttcagaagt
     5221 gctgtcagca accaatgatg tctttgaacg tctcatggac caagaggtcg gcgaaatcaa
     5281 ctatgataac atgcaccagc ttgtttttgc caataccaaa ctgactccca atccagacaa
     5341 caaggcagaa tttctcctct acgacaagga cgatacaggt gaggaagaag agagtcaaac
     5401 agaaacgaaa ctaacaggcg aaatgcgctt agttatcaag gagattctga aacttcatca
     5461 agaaaaaggt gttgccttta aggaaattgc ccttctgacc tccagccgca gtcgtaatga
     5521 ccagattctc ctcgccctgt ctgagtacgg aattcctgtc aaaactgacg gagagcaaaa
     5581 caattatctc caatccctag aagtgcaagt catgctagac actcttcgtg tcattcacaa
     5641 tcccctgcaa gactacgctt tggttgccct tatgaagtct ccaatgtttg gttttgatga
     5701 ggatgagcta gcacgtttgt cccttcagaa agcagaggat aaagtccacg aaaatctcta
     5761 tgagaaactg gtcaatgcac aaaaaatggc aagtagtcaa aaaggtttga ttcacacagc
     5821 tctagctgaa aaactaaagc aattcatgga tatcctagct tcttggcgct tgtatgccaa
     5881 aacccactct ctctatgact tgatttggaa gatttacaac gaccgttttt attatgacta
     5941 tgttggggct ttgccgaatg gtcctgctag gcaggccaat ctctatgccc tagcactgcg
     6001 tgctgatcaa tttgaaaaga gcaatttcaa aggtttgtcg cgttttattc gtatgattga
     6061 ccaagtctta gaagcccagc acgatttggc aagcgtggcc gtcgcaccgc caaaagatgc
     6121 agtagagctc atgaccatcc acaagagtaa agggctggag tttccttacg tctttatcct
     6181 caatatggat caagatttca acaagcaaga ctctatgtca gaagtcattc tcagtcgtca
     6241 gaatggtctt ggtgtcaaat atattgccaa gatggagaca ggggcagtag aagaccacta
     6301 tcctaaaacc atcaaactct ccattcctag tctgacctat aggcagaacg aagaggaatt
     6361 acagctagca agctattctg agcagatgcg tttgctgtat gttgctatga cgcgggctga
     6421 gaaaaagctc tatcttgtcg gcaagggttc tcgtgaaaag ctggaagtca agcaataccc
     6481 agcagccaaa aatgggaaac taaatagcaa tactagactg caagcacgga atttccaaga
     6541 ttggctttgg gctatcagta aagtgtttac taaggacaag ctcaacttta gttatcgttt
     6601 tattggcgaa gatcagttga ccagagaagc tatcggagag ttggaaacca agagtcctct
     6661 ccaagatagc tcccaagcag acaatcgtca gtcagatacc atcaaagaag ctctggaaat
     6721 gctgaaggag gtggaagttt ataatactct tcaccgcgca gctattgaac ttcctagtgt
     6781 tcaaacccca agtcaaatca agaaattcta cgaaccagtt atggatatgg aaggtgtcga
     6841 gattgctggt caaggtcagt cagtaggcaa gaaaatcagc ttcgatttgc cagatttttc
     6901 aaccaaagaa aaggtaactg gagctgagat tggtagtgct actcacgaac tcatgcagag
     6961 aattgacctc agccagcaac taacccttgc tagcctaaca gaaacactca aacaagttca
     7021 aactagccaa gctgtcagag acaagatcaa tcttgataaa attcttgctt tctttgacac
     7081 agtactcggt caggaaattc ttgctaatac cgaccatctc tatcgcgagc aacctttctc
     7141 catgctcaaa cgagaccaaa agagtcagga agactttgtt gtccgtggta tccttgatgg
     7201 ctatctgctt tacgaaaaca aaattgttct gttcgactac aagacagacc gctatgatga
     7261 accaagtcaa ctcgtagacc gctatcgtgg tcagttagct ctatacgaag aggctttatc
     7321 acgagcctat tcgattgaaa atattgaaaa atacttgatt ttactcggta aagacgaggt
     7381 tcaagttgta aaagtataac ttatactctt tgaaaatctc ttcaaaccac gtcagcttcg
     7441 ccttgctgta tatatggtta ctgacttcgt cagttctatc cacaacctca aaacagtgtt
     7501 ttgagctgac ttcgtcagtt ctatctacaa cctcaaaaca gtgttttgag ctgattgcgg
     7561 ctagctttct agtttgctct ttgattttta ttgagtatta gaaaggagac ctcatgacac
     7621 ttccagttag aaaatccctg cacgatgcag ttttacaggc ttcgaaagcg gatacttggg
     7681 aacaagctac caaggaatgg aatgaagttt ccttgatttt taacggtatc ggccgtagca
     7741 attgtgtctg tggaaatgcc atcaaatacg cttatgaact ctttaacggt gtcacaggcc
     7801 aacgcctctt tccaattggt agcgactgtg ttcgtcattt tcatcgattg tcccttgacc
     7861 agcaattgga agaggaagaa aaactgctca gaaaggttga aaatctaacc agaaaagctc
     7921 agaaaaagga aaaaatcaag gtcaataaaa gtgactttga cgagcgactt ctaaaatggc
     7981 tctgggaaaa aggtgttttc aaacccaatc gtggcaatca atttgcgcct gagagagact
     8041 accagctttt cctagaagtc tttcagggag gaagttggac caaggcggaa ccaaagaaaa
     8101 aggctcggat ggaagaagtc cttgaaaagt gtatcaaacc ctttttactt gggaaatcgg
     8161 atgaccaact ctaccttgtc aagctaggca aggagaaaat tgactacgaa caagaactcc
     8221 gtatccaggc agagaaagaa cgcaagaaga gggataaaat cgccaagcaa tatgcagaca
     8281 atctggttct agccatggga cccgcagagc gtgcttatca agattacttt ggctttacag
     8341 agaccctgac tcaagaagaa cgcaagtggg aaaaaattct ttttggtaaa aatagagcag
     8401 aacgggctat caaggccaaa caataccaaa aagagctgga aaaagaccaa cgaattgcca
     8461 gtcaggatcc aattgaaaga aaacagaagc agacttggct cctcaattcc tattttcgtg
     8521 agcttcctga agaaaaagct cgattttcta ggctcttatt agaatatcga aaaagtggtg
     8581 aagtaccctt ttcaactgaa tacctgtccg atcatctcat cgactttttc tataaaatga
     8641 aagcctttga atttgaaatc gcaccagaac aagtccgaga ttttctaaaa aaaagcctgc
     8701 aggaagacca tcgctcatca gcacaaggaa gctggataga aggaattctc ctcaactgcc
     8761 tcaaaccatt tttagaacga ttagtgatat gagtaacacc taccgtgtaa atttcatggt
     8821 aggtgttatt gtacccccaa ataatataaa acaaagtatg aaagcgcatc tcaattttaa
     8881 gacttttttg taaattaaga gtataattaa ggtatactgc cttttctata gataatagat
     8941 aatacactaa tataataaag gtctttgtaa catatataaa ttttatttca aggaggaata
     9001 atggaaaagt attttggtga aaaacaagag cgtttttcat ttagaaaatt atcagtagga
     9061 cttgtatctg caacgatttc aagtttattt tttatgtctg tattagctag ttcatctgtg
     9121 gatgctcaag aaactgcggg agttcactat aaatatgtgg cagattcaga gctatcatca
     9181 gaagaaaaga agcagcttgt ctatgatatt ccgacatacg tggagaatga tgatgaaact
     9241 tattatcttg tttataagtt aaattctcaa aatcaactgg cggaattacc aaatactgga
     9301 agcaagaatg agaggcaagc cctagttgct ggtgctagct tagctgctct gggaatttta
     9361 atttttgctg tttccaagaa aaaggttaag aataaaacgg tattacattt agtattggtt
     9421 gcgggaatgg gaaatggtgt cttagtttca gtccatgctt tagaaaatca tcttttgcta
     9481 aattacaata cggactatga attgacctct ggagaaaaat tacctcttcc taaagagatt
     9541 tcaggttaca cttatattgg atatatcaaa gagggaaaaa cgacttctga ttttgaagta
     9601 agtaatcaag aaaaatcagc agccactcct acaaaacaac aaaaggtgga ttataatgtt
     9661 acaccaaatt ttgtagacca tccatcaaca gtacaagcta ttcaggaaca aacacctgtt
     9721 tcttcaacta agccgacaga agttcaagta gttgaaaaac ctttctctac tgaattaatc
     9781 aatccaagaa aagaagagaa acaatcttca gattctcaag aacaattagc cgaacataag
     9841 aatctagaaa cgaagaaaga ggagaagatt tctccaaagg aaaagactgg ggtaaataca
     9901 ttaaatccac aggatgaagt gctatcaggt cagttgaaca aaccggaact cttatatcgt
     9961 gacgaaacta tagaaacaaa aatagatttt caagaagaaa ttcaagaaaa tcctgattta
    10021 gcagaaggaa ctgtaagagt aaaacaagaa ggtgcattag gtaagaaagt tgaaatcgtc
    10081 agaatatttt ctgtaaacaa tgaagaagtt tcacgagaaa ttatttcaac ttcaacgact
    10141 gcgcctgttt caagaatagt cgaaaaaggt actaaaaaag ctcaagttat aaaggaacaa
    10201 gctgagactg gtgcagaaca taaggaagta cagtctggag ctattgttga acccgcaatt
    10261 cagcctgaac taccagcagc tgtattaacc gacaaaggtg agtcagcagt ccaaccagag
    10321 ttgccagaag ctgtagtaag tgataaaggc gtaccagaag ttcaaccagc gttaccagag
    10381 gcagtcatga ctgacaaggg tgatccagag caggtagaac cgctaccaga atatacagga
    10441 gtacaagctg gagctattgt tgaacctgaa aaagttgaac cggaatatgc tggagtacaa
    10501 gctggcgcta ttgttgaacc agagcaggta gcaccactac cagaatacac aggggtgcaa
    10561 gccggagcga tagtcgagcc agagaaagta gaagcaccga aggaatacac aggagtgcag
    10621 gctggagcga tagtcgaacc agagaaggta gaagcaccga aggaatacac aggagtgcaa
    10681 gccggagcaa tagtagaacc agaaaaagta gaaccatcaa aagaatacac aggggtgcag
    10741 gctggcgcta ttgttgaacc agagcagata gcaccactac cagaatacac aggggtgcaa
    10801 gccggagcga tagtcgagcc agagaaagta gaagcactga aagaatatac tggtaaaatc
    10861 gaacaaccta gtgcagaaga tactaaacca aataatgaaa atactaatac accagaagaa
    10921 atgagtattc agaaaaaatc tagtgcatta attaatatga actttattac agattcgtcg
    10981 aaggttactg gagtaggatc agctactttt attgcaccaa atgtactttt aacagtagca
    11041 cataatttca ttaataattc tactgataat actactggcg aatttagagg tgataaatct
    11101 aaaaacgtat atgagtgggt gactcctgat ggacaaaaag gaacatttac tgctaacaat
    11161 attcattttt ataataaaaa agattatcca aaaggattta tctatgattt agcagtgatt
    11221 aagctacctg aaacaactgg cagagaacat gtagaattag ttaaaaatta ttcgaaagta
    11281 aatcttaatg acaaactaaa tgttcatgga tatccagctg ggaaatatac gcacttaaaa
    11341 gatgctacgg tagaaatgga acaagaatat gcgaataaca cctacggtgt tcaatatcaa
    11401 ggtggaaatc ctggtatgag tggtggagga atatttaatg ctaatggtga agttattggt
    11461 gtgcatcaaa atggagcaca aaatcgctca ggcggattga ttttatctcc tacacaatta
    11521 gcttggatta agagtattat agcaggtaat gaaattccac cagtatacga tgaactttat
    11581 cgtcataaag atgagaaaaa agatgacgct aaagatgaaa aagaagttat taagaaatta
    11641 gaacttagaa atatatcttc tgtagaacta tattcaaaag atggtaataa gtatcgacat
    11701 gtaacttcac ttgcttcttt accaagtaat gcagaaaatt actttatgaa agttaaatca
    11761 gagaacttta aagatgtgat gctaccagtt acaagcatta ctaatgatac taaagataat
    11821 agagatgttt ataagatagt agcaagtgca aatagtctga tacaacatga aaataataat
    11881 gtactagaaa attatacata ctatttacca aaaactcagc aaagtgaaac tggggtatat
    11941 acatcattta aaaatttagt agatgcaatg aatagcaatc ctaatggcac attccgttta
    12001 ggagcaacta tggatgctcg tgaagttgaa cttccagatg gtcaagaaag ttatgtgaat
    12061 aatgtattcc atggaatatt agtaggaaca aataatgaga aatattatgc tatttataat
    12121 ttgaaaaaac cattatttgg ggaattaaat ggtgctacag tagaaaaact ttcactaaaa
    12181 gatgttaata tttccgcaaa ggacgatact gcaacacttg cgaaagaagc aaataacaat
    12241 actcatattg ataacgttca tgctgatgga gcgatagctg gggaacgtag cataggagga
    12301 ttagtatcac aagttaataa ttctactatt tctaacagta gctatacagg tagaataact
    12361 aatacatata aaacagttgc tagttatcaa atcggaggtt tagtaggcaa actttctggt
    12421 ccgaggggat taatagataa gtcgttcgca tcaatcgatt tatcttcaaa tgcaacacaa
    12481 ggagaccagt ctattggtgg aatagttggt gcagtagaaa atagtgcgct tattagtaat
    12541 agttatgctg aaggaaatct aaataatgtt caacgttttg caaacgttgg tggagtagtt
    12601 ggtaatcttt gggatccggt tggaggatta gaaaaatccg gtcgactttc taatgtacta
    12661 agcgatgtta atgtaacgaa tggtaatgct attgcaggtt ataattttaa tggtataaaa
    12721 gcaaatggca catacagtaa taaaaataat aaggttgtga acgtagtaca agaagatgat
    12781 gaaatcttaa ctaaggattc tactgttcaa agaggagaag tattagaaga tgcacaaata
    12841 aaagagaaaa aagctacttt tgt
//
LOCUS       DAVKLX010000058        12621 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKLX010000058 DAVKLX010000000
VERSION     DAVKLX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.058"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            14..1150
                     /gene="dnaN"
                     /locus_tag="VW325_001817"
     CDS             14..1150
                     /gene="dnaN"
                     /locus_tag="VW325_001817"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9735159.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            1215..1409
                     /locus_tag="VW325_001818"
     CDS             1215..1409
                     /locus_tag="VW325_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9735160.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1493..2608
                     /gene="ychF"
                     /locus_tag="VW325_001819"
     CDS             1493..2608
                     /gene="ychF"
                     /locus_tag="VW325_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9735161.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2680..3249
                     /locus_tag="VW325_001820"
     CDS             2680..3249
                     /locus_tag="VW325_001820"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9735162.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            3250..6759
                     /gene="mfd"
                     /locus_tag="VW325_001821"
     CDS             3250..6759
                     /gene="mfd"
                     /locus_tag="VW325_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9735163.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            6817..7083
                     /locus_tag="VW325_001822"
     CDS             6817..7083
                     /locus_tag="VW325_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9735164.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7076..7444
                     /locus_tag="VW325_001823"
     CDS             7076..7444
                     /locus_tag="VW325_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9735165.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7564..8832
                     /locus_tag="VW325_001824"
     CDS             7564..8832
                     /locus_tag="VW325_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9735166.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFSIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8829..10106
                     /gene="tilS"
                     /locus_tag="VW325_001825"
     CDS             8829..10106
                     /gene="tilS"
                     /locus_tag="VW325_001825"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9735167.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIINTVLYIEKIDR"
     gene            10110..10652
                     /gene="hpt"
                     /locus_tag="VW325_001826"
     CDS             10110..10652
                     /gene="hpt"
                     /locus_tag="VW325_001826"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9735168.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10668..>12621
                     /gene="ftsH"
                     /locus_tag="VW325_001827"
     CDS             10668..>12621
                     /gene="ftsH"
                     /locus_tag="VW325_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9735169.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         4345 a         1900 c         2566 g         3810 t
ORIGIN      
        1 taaaggagaa tccatgattc atttttcaat taataaaaat ttatttctac aagcattaaa
       61 tactactaag agagctatta gttctaaaaa tgccattcct attttatcaa cagtaaaaat
      121 tgacgtgacc aatgaaggta ttactttaat tggttcaaat ggtcaaattt caattgaaaa
      181 ttttatttct caaaaaaatg aagatgctgg tttgttaatt acttctttag gttcgatcct
      241 tcttgaagct tctttcttta tcaatgtagt atctagttta cctgatgtga ctcttgattt
      301 taaagaaatt gaacaaaatc aaattgtttt aaccagtggc aaatcagaaa ttaccctaaa
      361 aggaaaagat agcgaacaat atccacgaat ccaagaaatt tcagcaagca ctcctttaat
      421 acttgaaaca aaattactca agaaaattat taatgaaaca gcctttgctg caagtacaca
      481 agagagtcgt ccgattttaa caggtgtcca cttcgtattg agtcaacaca aagagttaaa
      541 aacagttgca acagactctc atcgcctaag ccagaaaaaa ttgactcttg aaaaaaatag
      601 tgatgatttt gatgtcgtaa ttcctagccg ttctctacgc gaattttcag cggtatttac
      661 agatgatatc gaaactgtag agattttctt tgccaataac caaatcctct ttagaagcga
      721 aaatattagc ttctatactc gtctcctaga aggaaactat cctgatacag atcgcttgat
      781 tccaacagac tttaacacta ctattacttt taatgtggta aacttacgcc agtcaatgga
      841 gcgtgcccgt cttttatcaa gtgcgactca aaatggtact gtgaaacttg aaattaagga
      901 tggggttgtt agcgcccatg ttcactctcc agaagttggt aaagtaaacg aagaaatcga
      961 tactgatcag gttactggtg aagatttgac cattagtttc aacccaactt acttgattga
     1021 ttctcttaaa gctttaaata gcgaaaaggt gaccattagc tttatctcag ctgttcgtcc
     1081 atttactctt gtgccagcag atactgacga agacttcatg cagctcatta caccagttcg
     1141 tacaaattaa gtgaaagagg ttgagcctgg ctcgcctctt ttatgatata atcgaaaaag
     1201 aaaaggagag tagtatgtat caagttggaa attttgttga gatgaaaaaa tcacacgctt
     1261 gtacaatcaa gtcgactggt aaaaaggcta atcgttggga aattacacgt gtaggagcag
     1321 atatcaaaat aaaatgcagt aattgtgagc atgttgtcat gatggggcga tatgattttg
     1381 agcgaaaaat gaataaaatt attgactgag aacccttagt tagagggtta gcactttatc
     1441 cctttttgtg ttataatatt agggattgaa atgaaaacgg agaatgagaa atatggcttt
     1501 gacagcaggt atcgttggtt tgccaaacgt tggtaaatca acattattta atgcaattac
     1561 aaaagcagga gcagaggcag caaactaccc atttgcgact attgatccaa atgttggaat
     1621 ggtggaagtt ccagatgaac gcctacaaaa actaactgaa atgataactc ctaaaaagac
     1681 agttcccaca acatttgaat ttacagatat tgcagggatt gtaaaaggag cttcaaaagg
     1741 agaagggcta gggaataaat tcttggccaa tattcgtgaa gtagatgcga ttgttcacgt
     1801 agttcgtgct tttgatgatg aaaatgtgat gcgcgagcaa ggacgtgaag acgcctttgt
     1861 agatccactt gcagatattg atacaattaa tctggaattg attcttgctg acttagaatc
     1921 agtgaacaaa cgatatgcgc gtgtagaaaa gatggcacgt acgcaaaaag ataaagaatc
     1981 agtagcagaa ttcaatgttc ttcaaaagat taaaccagtc ctagaagacg ggaaatcagc
     2041 tcgtaccatt gaatttacag atgaggaaca aaaggttgtc aaaggtcttt tccttttgac
     2101 gactaaacca gttctttatg tagctaatgt ggacgaggat gtggtttcag aacctgactc
     2161 tatcgactat gtcaaacaaa ttcgtgaatt tgcagcgaca gaaaatgctg aagtagtcgt
     2221 tatttctgcg cgtgctgagg aagaaatttc tgaattggat gatgaagata aaaaagagtt
     2281 tcttgaagcc attggtttga cagaatcagg tgtagataag ttgacgcgtg cagcttacca
     2341 cttgcttgga ttgggaactt acttcacagc tggtgaaaaa gaagttcgcg cttggacttt
     2401 caaacgtggt atgaaggctc ctcaagcagc tggtattatc cactcagact ttgaaaaagg
     2461 ctttattcgt gcagtaacca tgtcatatga agatctagtg aaatacggat ctgaaaaggc
     2521 cgtaaaagaa gctggacgct tgcgtgaaga aggaaaagaa tatatcgttc aagatggcga
     2581 tatcatggaa ttccgcttta atgtctaaaa attaataaat ggtgtcaatt aggttggaaa
     2641 aaaaattcca acccttttgg cttttgaaag gaaaaataaa tgaccaaatt acttgtaggc
     2701 ttgggaaatc caggggataa atattttgaa acaaaacaca atgttggttt tatgttgatt
     2761 gatcaactag cgaagaaaca gaatgtcact tttacacacg ataagatatt tcaagctgac
     2821 ctagcatcct ttttcctaaa tggagaaaaa atttatctgg ttaaaccaac gacctttatg
     2881 aatgaaagtg gaaaagcagt tcatgcttta ttaacttact atggtttgga tattgacgat
     2941 ttacttatca tttacgatga tcttgacatg gaagttggga aaattcgttt aagagcaaaa
     3001 ggctcagcag gtggtcataa tggtatcaag tctattattc aacatatagg aactcaggtc
     3061 tttaaccgtg ttaagattgg aattggaaga cctaaaaatg gtatgtcagt tgttcatcat
     3121 gttttgagta agtttgacag ggatgattat atcggtattt tacagtctgt tgacaaagtt
     3181 gacgattctg taaaatacta tttacaagag aaaaattttg agaaaacaat gcagaggtat
     3241 aacggataaa tggtgacctt attagattta ttctcagaaa atgatcagat taaaaaatgg
     3301 catcaaaatt taacagataa gaaaagacaa ctaatacttg gtttatcaac atctactaag
     3361 gctcttgcaa ttgcaagcag tttagaaaaa gaagatagga ttgtgttatt gacgtcaact
     3421 tatggagaag cagaaggact tgttagtgat cttatttcta tcttgggtga ggaactcgtc
     3481 tatccatttt tggtagatga tgctcctatg gtggagtttt tgatgtcttc acaggaaaaa
     3541 attatttcac gggttgaagc cttgcgtttt ttgactgatt catctaagaa agggatttta
     3601 gtttgtaata tcgcagcaag tcgattgatt ttaccgtctc ccaatgcatt caaagatagt
     3661 attgtaaaaa tctcagttgg tgaagaatat gatcaacacg cgtttatcca tcagttaaag
     3721 gaaaatggct atcgaaaagt tactcaagta caaactcagg gcgaatttag tcttcgagga
     3781 gatattttag atatttttga aatatcccag ttagaacctt gtcgaattga gttttttggt
     3841 gatgaaattg atggtatcag gtcatttgaa gtagaaacac aattatcgaa agaaaataag
     3901 acagaactca ctatctttcc agctagtgat atgcttttga gagaaaagga ttatcaacga
     3961 ggacagtcag ctttagaaaa acaaatttca aaaactttat cacctatttt gaaatcatac
     4021 ctagaagaaa ttctttcaag ttttcaccaa aaacaaagtc atgcagactc tcggaagttt
     4081 ttatctttgt gctatgataa gacatggact gtctttgatt atattgaaaa agatactcca
     4141 atattctttg atgattatca aaaattgatg aatcagtatg aagtctttga aagagactta
     4201 gcgcagtact ttacagaaga attacagaat agtaaagcat tttctgatat gcagtatttt
     4261 tctgatattg aacaaatcta taaaaaacaa agtccagtga cctttttctc taatcttcaa
     4321 aagggtttag gaaatctcaa atttgacaaa atttatcaat ttaatcaata tcctatgcag
     4381 gaatttttca atcagttttc ttttctaaaa gaagaaattg aacgatataa aaaaatggat
     4441 tacaccatta ttctgcagtc tagcaattca atgggaagta aaacattgga ggatatgtta
     4501 gaggaatatc agattaaatt ggattctaga gataagacaa gtatctgtaa agaatctgta
     4561 aacttaatag agggtaatct cagacatggt tttcattttg tagatgaaaa gattttattg
     4621 ataactgaac atgagatttt tcaaaagaaa ttaaagcgtc gttttcgaag acaacatgtt
     4681 tcaaatgcag agagattaaa agattacaat gaacttgaaa aaggggacta tgttgtccat
     4741 catatccatg ggattggtca atatctagga attgaaacca ttgaaatcaa gggaattcat
     4801 cgcgattatg tcagtgtcca ataccaaaat ggtgatcaaa tttctatccc cgtggaacag
     4861 attcatctac tgtccaaata tatttcaagt gatggtaaag ctccaaaact caataaatta
     4921 aatgacggtc attttaaaaa ggccaagcaa aaggttaaga accaggtaga ggatatagct
     4981 gatgatttaa tcaaactcta ctctgaacgt agtcagttga agggttttgc tttctcagct
     5041 gatgatgatg atcaagatgc ctttgatgat gctttccctt atgttgaaac ggatgatcaa
     5101 cttcgtagta ttgaggaaat caagagggat atgcaggctt ctcagccaat ggatcgactt
     5161 ttagttgggg atgttggttt tggaaagact gaagttgcta tgcgtgcagc ctttaaagca
     5221 gtcaatgatc acaaacaggt tgtcattcta gttccgacga cggttttagc gcaacagcac
     5281 tatacgaatt ttaaggaacg attccaaaat tttgcagtta atgttgatgt gttgagtcgc
     5341 tttagaagta aaaaagagca gactgcaaca cttgaaaaat tgaaaaacgg tcaagtcgat
     5401 attttgattg gaacacatcg tgttttgtca aaagatgttg tgtttgctga tttgggcttg
     5461 atgattattg atgaggaaca gcgatttggt gtcaagcata aggaaacttt gaaagaactg
     5521 aagaaacaag tggatgtcct aaccttgacc gctacgccaa tccctcgtac cctccatatg
     5581 tctatgctgg gaatcagaga tttatctgtt attgaaactc cgccgactaa tcgctatcct
     5641 gttcagacct atgttttgga aaagaatgat agtgtcattc gtgatgctgt cttgcgtgaa
     5701 atggagcgtg gaggtcaagt ttattatctt tacaacaaag ttgacacaat tgttcagaag
     5761 gtttcagaat tacaggagtt gattccggag gcttcgattg gatatgttca tggtcgaatg
     5821 agtgaagtcc agttggaaaa tactctatta gactttattg agggacaata cgatatcttg
     5881 gtgacgacta ctattattga gacaggggtg gacattccaa atgctaatac tttatttatt
     5941 gaaaatgcgg accatatggg cttgtcaacc ttatatcagt taagaggaag agtcggtcgt
     6001 agtaatcgta ttgcttatgc ttatctcatg tatcgtccag aaaaatcaat cagtgaagtc
     6061 tctgaaaaga gattagaagc gattaaagga tttacagaat tgggctctgg ctttaagatt
     6121 gcaatgcgag atctttcgat tcgtggagca ggaaatcttt taggaaaatc ccagtctggt
     6181 ttcattgatt ctgttggttt tgaattgtat tcgcagttat tagaggaagc tattgctaaa
     6241 cgaaacggta atgctaacgc taacacaaga accaaaggga atgctgagtt gattttgcaa
     6301 attgatgcct atcttcctga tacttatatt tctgatcaac gacataagat tgaaatttac
     6361 aagaaaattc gtcaaattga caaccgtgtc aattatgaag agttacaaga ggagttgata
     6421 gaccgttttg gagaataccc agatgtagta gcctatcttt tagagattgg tttggttaaa
     6481 tcatacttgg acaaggtctt tgttcaacgt gtggaaagaa aagataataa aattacaatt
     6541 caatttgaaa aagtcactca acggctgttt ttagctcaag attattttaa agctttatcc
     6601 gtaacgaact taaaagcagg catcgctgag aataagggat taatggagct tgtatttgat
     6661 gtccaaaata agaaagatta tgaaatttta gaaggtctgc tgatttttgg agaaagttta
     6721 ttagagataa aagagtttaa ggaagaaaat tccatttgat atttttcttc tataaaatag
     6781 ataaaaatgg tacaataata aattgaggta ataaggatga gattagataa atatttaaaa
     6841 gtatcgcgaa ttatcaagcg tcgtacagtc gcaaaggaag tagcagataa aggtagaatc
     6901 aaggttaatg gaatcttggc caaaagttca acggacttga aagttaatga ccaagttgaa
     6961 attcgctttg gcaataagtt gctgcttgta aaagtactag agatgaaaga tagtacaaaa
     7021 aaagaagatg cagcaggaat gtatgaaatt atcagtgaaa cacgggtaga agaaaatgtc
     7081 taaaaatatt gtacaattga ataattcttt tattcaaaat gaataccaac gtcgtcgcta
     7141 cctgatgaaa gaacgacaaa aacggaatcg ttttatggga ggggtattga ttttgattat
     7201 gctattattt atcttgccaa cttttaattt agcgcagagt tatcagcaat tactccaaag
     7261 acgtcagcaa ttagcagact tgcaaactca gtatcaaact ttgagtgatg aaaaggataa
     7321 ggagacagca tttgctacca agttgaaaga tgaagattat gctgctaaat atacacgagc
     7381 gaagtactat tattctaagt cgagggaaaa agtttatacg attcctgact tgcttcaaag
     7441 gtgataaaat ggaaaattta ttagacgtaa tagagcaatt tttgagtttg tcagatgaaa
     7501 agctggaaga attggctgat aaaaatcaat tattgcgttt acaagaagaa aaggaaagga
     7561 agaatgcgta aattcttaat tattttgttg ctaccaagtt ttttgaccat ttcaaaagtc
     7621 gttagcacag aaaaagaagt cgtctatact tcgaaagaaa tttattacct ttcacaatct
     7681 gactttagta tttattttag agaaaaatta agttctccca tggtttatgg agaggttcct
     7741 gtttatgcga atgaagattt agtagtggaa tctgggaaat tgactcccaa aacaagtttt
     7801 caaataaccg agtggcgctt aaataaacaa ggaattccag tatttaagct atcaaatcat
     7861 caatttatag ctgcggacaa acgattttta tatgatcaat cagaggtaac tccaacaata
     7921 aaaaaagcat ggttagaatc tgactttaaa ctgtacaata gtccttatga tttaaaagaa
     7981 gtgaaatcat ccttatcagc ttattcgcaa gtatcaatcg acaagaccat gtttgtagaa
     8041 ggaagagaat ttctacatat tgatcaggct ggatgggtag ctaaagaatc aacttctgaa
     8101 gaagataatc ggatgagtaa agttcaagaa atgttatctg aaaaatatca gaaagattct
     8161 ttctctattt atgttaagca actgactact ggaaaagaag ctggtatcaa tcaagatgaa
     8221 aagatgtatg cagccagcgt tttgaaactc tcctatctct attatacgca agaaaaaata
     8281 aatgagggtc tttatcagtt agatacgact gtaaaatacg tatctgcagt caatgatttt
     8341 ccaggttctt ataaaccaga gggaagtggt agtcttccta aaaaagaaga taataaagaa
     8401 tattctttaa aggatttaat tacgaaagta tcaaaagaat ctgataatgt agctcataat
     8461 ctattgggat attacatttc aaaccaatct gatgccacat tcaaatccaa gatgtctgcc
     8521 attatgggag atgattggga tccaaaagaa aaattgattt cttctaagat ggccgggaag
     8581 tttatggaag ctatttataa tcaaaatgga tttgtgctag agtctttgac taaaacagat
     8641 tttgatagtc agcgaattgc caaaggtgtt tctgttaaag tagctcataa aattggagat
     8701 gcggatgaat ttaagcatga tacgggtgtt gtctatgcag attctccatt tattctttct
     8761 attttcacta agaattctga ttatgatacg atttctaaga tagccaagga tgtttatgag
     8821 gttctaaaat gagggaacca gattttttaa atcattttct caagaaggga tatttcaaaa
     8881 agcatgctaa ggcggttcta gctctttctg gtggattaga ttccatgttt ctatttaagg
     8941 tattgtctac ttatcaaaaa gagttagaga ttgaattgat tctagctcat gtgaatcata
     9001 agcagagaat tgaatcagat tgggaagaaa aggaattaag gaagttggct gctgaagcag
     9061 agcttcctat ttatatcagc aatttttcag gagaattttc agaagcgcgt gcacgaaatt
     9121 ttcgttatga tttttttcaa gaggttatga aaaagacagg tgcgacagct ttagtcactg
     9181 cccaccatgc tgatgatcag gtggaaacga ttttgatgcg cttgattcga ggaactcgct
     9241 tgcgctatct atcaggaatt aaggagaagc aagtagtcgg agagatagaa atcattcgtc
     9301 ccttcttgca ttttcagaaa aaagactttc catcaatttt tcactttgaa gatacatcaa
     9361 atcaggagaa tcattatttt cgaaatcgta ttcgaaactc ttacttacca gaattggaaa
     9421 aagaaaatcc tcgatttagg gatgcaatct taggcattgg caatgaaatt ttagattatg
     9481 atttggcaat agctgaatta tctaacaata ttaatgtgga agatttacag cagttatttt
     9541 cttactctga gtctacacaa agagttttac ttcaaactta tctgaatcgt tttccagatt
     9601 tgaatcttac aaaagctcag tttgctgaag ttcagcagat tttaaaatct aaaagccagt
     9661 atcgtcatcc gattaaaaat ggctatgaat tgataaaaga gtaccaacag tttcagattt
     9721 gtaaaatcag tccgcaggct gatgaaaagg aagatgaact tgtgttacac tatcaaaatc
     9781 aggtagctta tcaaggatat ttattttcct ttggacttcc attagaaggt gaattaattc
     9841 aacaaatacc tgtttcacgt gaaacatcca tacacattcg tcatcgaaaa acaggagatg
     9901 ttttgattca aaatgggcat agaaaaaaac tcagacgttt atttattgat ttgaaaatcc
     9961 ctatggaaaa gagaaactct gctcttatta ttgagcaatt tggtgaaatt gtctcaattt
    10021 tgggaattgc gaccaataat ttgagtaaaa aaacgaaaaa tgatataatt aacactgtac
    10081 tttatataga aaaaatagat aggtaaaaaa tgttagaaaa cgatattaaa aaagtcctcg
    10141 tttcacacga tgaaattaca gaagcagcta aaaaactagg tgctcaatta actaaagact
    10201 atgcaggaaa aaatccaatc ttagttggga ttttaaaagg atctattcct tttatggctg
    10261 aattggtcaa acatattgat acacatattg aaatggactt catgatggtt tctagctacc
    10321 atggtggaac agcaagtagt ggtgttatca atattaaaca agatgtgact caagatatca
    10381 aaggaagaca tgttctattt gtagaagata tcattgatac aggtcaaact ttgaagaatt
    10441 tgcgagatat gtttaaagaa agagaagcag cttctgttaa aattgcaacc ttgttggata
    10501 aaccagaagg acgtgttgta gaaattgagg cagactatac ctgctttact atcccaaatg
    10561 agtttgtagt aggttatggt ttagactaca aagaaaatta tcgtaatctt ccttatattg
    10621 gagtattgaa agaggaagtg tattcaaatt agaaagaata atctttaatg aaaaaacaaa
    10681 ataatggttt aattaaaaat ccttttctat ggttattatt tatctttttc cttgtgacag
    10741 gattccagta tttctattct gggaataact caggaggaag tcagcaaatc aactatactg
    10801 agttggtaca agaaattacc gatggtaatg taaaagaatt aacttaccaa ccaaatggta
    10861 gtgttatcga agtttctggt gtctataaaa atcctaaaac aagtaaagaa ggaacaggta
    10921 ttcagttttt cacgccatct gttactaagg tagagaaatt taccagcact attcttcctg
    10981 cagatactac cgtatcagaa ttgcaaaaac ttgctactga ccataaagca gaagtaactg
    11041 ttaagcatga aagttcaagt ggtatatgga ttaatctact cgtatccatt gtgccatttg
    11101 gaattctatt cttcttccta ttctctatga tgggaaatat gggaggaggc aatggccgta
    11161 atccaatgag ttttggacgt agtaaggcta aagcagcaaa taaagaagat attaaagtaa
    11221 gattttcaga tgttgctgga gctgaagaag aaaaacaaga actagttgaa gttgttgagt
    11281 tcttaaaaga tccaaaacga ttcacaaaac ttggagcccg tattccagca ggtgttcttt
    11341 tggagggacc tccggggaca ggtaaaactt tgcttgctaa ggcagtcgct ggagaagcag
    11401 gtgttccatt ctttagtatc tcaggttctg actttgtaga aatgtttgtc ggagttggag
    11461 ctagtcgtgt tcgctctctt tttgaggatg ccaaaaaagc agcaccagct atcatcttta
    11521 tcgatgaaat tgatgctgtt ggacgtcaac gtggagtcgg tctcggcgga ggtaatgacg
    11581 aacgtgaaca aaccttgaac caacttttga ttgagatgga tggttttgag ggaaatgaag
    11641 ggattatcgt catcgctgcg acaaaccgtt cagatgtact tgatcctgcc cttttgcgtc
    11701 caggacgttt tgatagaaaa gtattggttg gccgtcctga tgttaaaggt cgtgaagcaa
    11761 tcttgaaagt tcacgctaag aacaagcctt tagcagaaga tgttgatttg aaattagtgg
    11821 ctcaacaaac tccaggcttt gttggtgctg atttagagaa tgtcttgaat gaagcagctt
    11881 tagttgctgc tcgtcgcaat aaatcgataa ttgatgcttc agatattgat gaagcagaag
    11941 atagagttat tgctggacct tctaagaaag ataagacagt ttcacaaaaa gaacgagaat
    12001 tggttgctta ccatgaggca ggacatacca ttgttggtct agtcttgtcg aatgctcgcg
    12061 ttgtccataa ggttacaatt gtaccacgcg gccgtgcagg cggatacatg attgcacttc
    12121 ctaaagagga tcaaatgctt ctatctaaag aagatatgaa agagcaattg gctggcttaa
    12181 tgggtggacg tgtagctgaa gaaattatct ttaatgtcca aactacagga gcttcaaacg
    12241 actttgaaca agcgacacaa atggcacgtg caatggttac agagtacggt atgagtgaaa
    12301 aacttggccc agtacaatat gaaggaaacc atgctatgtt tggtgcacag aatcctcaaa
    12361 aatcaatttc agaacaaaca gcttatgaaa ttgatgaaga ggttcgttca ttattaaatg
    12421 aggcacgaaa taaagctgct gaaattattc agtcaaatcg tgaaactcac aagttaattg
    12481 cagaagcatt attgaaatac gaaacattgg atagtacaca aattaaagct ctttacgaaa
    12541 caggaaagat gcctgaagca gtagaagagg aatctcatgc actatcctat gatgaagtaa
    12601 agtcaaaaat gaatgacgaa a
//
LOCUS       DAVKLX010000059        12008 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKLX010000059 DAVKLX010000000
VERSION     DAVKLX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12008
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.059"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            164..817
                     /locus_tag="VW325_001828"
     CDS             164..817
                     /locus_tag="VW325_001828"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9735170.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            827..1291
                     /locus_tag="VW325_001829"
     CDS             827..1291
                     /locus_tag="VW325_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9735171.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1304..2572
                     /locus_tag="VW325_001830"
     CDS             1304..2572
                     /locus_tag="VW325_001830"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9735172.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2586..2846
                     /locus_tag="VW325_001831"
     CDS             2586..2846
                     /locus_tag="VW325_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735173.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2857..4218
                     /gene="pdrM"
                     /locus_tag="VW325_001832"
     CDS             2857..4218
                     /gene="pdrM"
                     /locus_tag="VW325_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9735174.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4372..5340
                     /locus_tag="VW325_001833"
     CDS             4372..5340
                     /locus_tag="VW325_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9735175.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGSVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5356..6348
                     /locus_tag="VW325_001834"
     CDS             5356..6348
                     /locus_tag="VW325_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9735176.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEEISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCSGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            6566..7609
                     /locus_tag="VW325_001835"
     CDS             6566..7609
                     /locus_tag="VW325_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9735177.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHSYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7673..9358
                     /gene="lpdA"
                     /locus_tag="VW325_001836"
     CDS             7673..9358
                     /gene="lpdA"
                     /locus_tag="VW325_001836"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9735178.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9422..10411
                     /locus_tag="VW325_001837"
     CDS             9422..10411
                     /locus_tag="VW325_001837"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9735179.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEAVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10484..11554)
                     /gene="xerS"
                     /locus_tag="VW325_001838"
     CDS             complement(10484..11554)
                     /gene="xerS"
                     /locus_tag="VW325_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9735180.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3562 a         2241 c         2666 g         3539 t
ORIGIN      
        1 atttaaaaag taattataaa aaatagccag tctttataaa ctgtattcac gtattcaaat
       61 cgtatatata gtcgatttat taaatcttta gataaactgt aaaaagtggt ttccatagcc
      121 actttttgct ataatagagg cagtaaaaat gaaggagtcg gctatggaac actcgtcttg
      181 gcatgctttg attaaggcgc aattacctga gggttatttc gggaaaatca atcagtttat
      241 ggagcaggtc tattctcagg ggattattta tccacccaag gaaaaggttt ttcaggctct
      301 cttgacaaca ctgcttgaag aagttaaggt ggtaattcta gggcaagacc cctatcacgg
      361 accaggtcaa gcgcagggct tgagtttttc tgtacctgac tctatcccag ctccaccatc
      421 cttgcaaaat atcttgaaag aattgtcaga tgatatcgga gttaagaaat ctcatgattt
      481 gacagcttgg gctgagcaag gagtcttgct tcttaatgct tgtttgactg ttcctgctgg
      541 acaggccaat ggtcatgctg gtcaaatctg ggagcctttt actgatgctg tgattcaggt
      601 ggtcaatcat ctagatagac cagtcgtttt tgtactctgg ggagcttatg cacgtaagaa
      661 gaaggcatta gttaccaatc ctcatcactt gattatcgaa tcagcccatc caagtccttt
      721 gtcggtttat agaggatttt ggggttccaa gcccttttcc aaggccaatg cattcttaaa
      781 agagacagga caagagccaa tcgattggct tagataagga gaaaatatgc ctcagttagc
      841 gacgatttgc tacattgata atgggaaaga actgctcatg ctccatcgta ataagaaacc
      901 caatgatgtc catgaaggga aatggattgg tgtgggtggt aagctagaga gaggagagac
      961 gccccaggaa tgcgcggcgc gtgaaatcct tgaagaaaca gggctcaaag ccaagccagt
     1021 tctaaaaggt gtcatcactt ttcctgaatt tacaccagat ttagactggt acacctatgt
     1081 ttttaaggtg acggagtttg agggcgactt gattgactgc aatgagggga cgctagaatg
     1141 ggttccctat gatgaggttt tgagcaagcc gacttgggaa ggtgaccaca cctttgttga
     1201 gtggctttta gaggataaac ccttcttttc agccaagttt gtttatgatg gggataaatt
     1261 gttggatacc caagttgatt tctatgaata aaggagaaag cagatgctac taatcaaaaa
     1321 tggtcgtgta atggatccca agtctggttt ggatcaagtt tgtgatgtct tagttcaaga
     1381 tgggaaaatt atcaaaattg cgtctgagat cacggaagaa ggagcagaaa cgattgatgc
     1441 tactggtctt gtggttgctc ctggcttggt cgatattcat gttcatttcc gtgaacctgg
     1501 tcaaacacat aaagaagaca ttcatactgg tgccctagca gccgctgcag gtggttttac
     1561 tactgtcgtc atgatggcta ataccagtcc aaccatttca gacgtggaga ctttgcaagc
     1621 agttctccag tcagctgcca aagagaagat taatgtcaag acagttgcga ccattactaa
     1681 aaactttaat ggtaaaaact tgactgactt taaggcactc ttagaagctg gtgcggttgg
     1741 tttctctgat gacggtattc cgcttgagag cagtaagatt gtcaaggaag ccatggagga
     1801 agccaaaaaa ctcaatacct ttatcagcct tcatgaggaa gatccaggtt tgaacggtgt
     1861 tcttggcttt aatgaaaata ttgctagaga acatttccat atctgcggtg ctactggggt
     1921 ggctgagtac gctatgatgg cgcgtgatgt catgattgcc tatgcaacta aagcccatgt
     1981 tcacatccag catttgtcta aggaagaaag tgttaaagta gtggagtttg ctcaggggtt
     2041 aggtgcagaa gtcacagcag aagtagcgcc acagcatttc tctaagacag aagcacttct
     2101 tttaacacaa ggtagcaatg ctaagatgaa tccaccgctt cgtttggaat cagaccgtcg
     2161 tgccgttatc gaaggtctca aatcaggtgt catcacagtt attgcgactg accacgcgcc
     2221 tcatcatgta gatgaaaaaa atgttgagga tattaccaaa gcgccatctg gtatgactgg
     2281 cttagaaaca tccctgtctc tcagcttgac ctatttagta gaagctggtg agttgagctt
     2341 gatggaatta cttgaaaaaa tgacatacaa cccagccaag ctttacaact ttgaagcagg
     2401 ttacttggct gagaatggtc cagcagatat cactattttt gatgccaagg ctgaccgctt
     2461 tgtggactcc cattttgctt ccaaagcagc taattcacca ttcatcggtg aaaccttaaa
     2521 agggcaggtt aaatatacca tctgtaaggg acaaatcgtc tatcaagctt gataggagtt
     2581 agaatatgaa ttattttcga aaacgtaggg agagacaagc gaaatctaat agtggaattt
     2641 actgtcccgc agctaatcgt tccaaaattc aatatgaaac taaagaaaaa gctgaccatg
     2701 cggttcaata tgataacgga ggtttagtta gaagctatta ttgtcgtaca tgtgcgtgtt
     2761 ggcatactac ttcaaaatct aataaacctc cgctaagttt gaaaaactat ggtctgaaac
     2821 tctttggttt agataaatca gaatagaaag agaagtatgt ataagacaaa gtgtttacga
     2881 gagaagttag tattattttt aaaaattttc ttcccaatcc tgatctacca atttgccaat
     2941 tattctgcct cttttgttga tactgcaatg acaggtcaat acaacactat ggacttggct
     3001 ggtgtatcta tggcaaccag tatctggaat cctttcttta catttctaac agggattgtg
     3061 tcagccttgg tgcctatcat tggtcaccat cttggtcgag gcaaaaagga agaagttgcg
     3121 tctgattttt accaatttat ttatttggcc ttgggcctat ctgtggtctt gctggggatg
     3181 gtacttttct tggcaccaat aatcttgaat catattgggt tagaagcagc agtagcggca
     3241 gtagcggttc gctatctttg gtttttatct atcgggatta tccccttgtt gctctttagc
     3301 gtcattcgtt ccttgctgga ttcgctgggc ttgaccaaac tgtccatgta cctcatgctt
     3361 ttgttactcc ctctcaatag cggatttaac tatctcttga tttacggtgc ctttggtgtt
     3421 ccagaactgg gaggggctgg tgctggttta ggaacatcct tggcctactg ggtcttgctt
     3481 gggatttctg ttctggtttt atttaaacag gagaagctca aagccttaca ccttgagaaa
     3541 cgaattccac ttaatatgga taacattaag gaaggagttc gtttaggtct gcctattggg
     3601 ggaactgtct tcgcggaagt ggctatcttt tcagtggttg gcttgattat ggctaagttt
     3661 tcgtccttga ttatagctag tcaccagtca gctatgaact tttcaagtct tatgtacgcc
     3721 tttcctatga gtatctcatc ggctatggct attgtcgttt cctatgaagt gggagccaag
     3781 cgatttgatg atgcgaaaac ctatattggt ctaggaagat ggactgctct catttttgcg
     3841 gccttcaccc taaccttcct ttacattttt aggggaaatg tggccagtct ttatggtaac
     3901 gacccaaaat ttatcgattt gacagcgcgt tttttaactt atagtctttt cttccagtta
     3961 gcagatacct ttgcggcacc gcttcaggga attttgcggg ggtataagga tacagttatt
     4021 cctttttacc ttggtttgct tggttattgg ggcgtagcaa tccctgtggc tacgctattt
     4081 gattccctaa cagattttgg agcttattct tactggatcg gcttgattat tagtttgatt
     4141 gtgagtgggg cgctctaccg ttggcgttta actgtgatta tgaagagatt tgaatcttta
     4201 gcaaaatcca aacgctaaaa agtttgtgaa aaaatattct tgataagaaa atcccttgtt
     4261 ttcacggggt tttctttttt attttgtgaa atttctttta gaaaaacttt gacagtattt
     4321 gccaaaagcg gtaaaatagt aaggtaagaa gaaagttaga aaggcaagaa gatgtcaact
     4381 ttagataaaa atcttttgct agaaatgttc cgtaagatgg aagaaatccg tcgcatggac
     4441 ttaaaaattg cacaattagt aaagaaaggg aaagtgccag gaatgacgca cttttctgtt
     4501 ggtgaagagg cagctaacgt gggggctatg ttagctctca atccagatga tctgattacc
     4561 tcaaaccacc gtggacacgg gcaagctatt gctaaaggga ttgacctcaa cggaatgatg
     4621 gctgaaatcc ttggtaaata cactggaacc tgtaaaggga aaggtggatc tatgcatatc
     4681 gctgaccttg atgctgggaa ccttggtgcc aatggtatcg taggtggtgg tatggggatc
     4741 gctgtcggtg cagccctcag tcagcaaatg caaaataccg gtaaaatcgt tgtctgcttc
     4801 tttggagatg gtgcgaccaa tgaaggtgtt ttccacgaag cagtgaacat ggcttctatc
     4861 tggaacctgc cagtcatttt ctattgcatt aacaacggtt acggtatctc tgcggatatc
     4921 aagaaaatga ccaatataga acatatccat caacgtagcg ccgcttatgg aattcctgga
     4981 atgttcatcg aagatggtaa caatgtcatc gatgtctatg aaggatttca gaaagctgtg
     5041 gaccatgttc gcagtggcaa tggttcagtc ttgattgaaa gtgtaactta tcgctggctc
     5101 ggtcactcat catctgaccc tggtaaatat cgtacgcgtg aagaagtgga attgtggaaa
     5161 caaaaagatc caatcgaaaa cctccgcaat taccttattg aaaataacat tgcaagtgcc
     5221 gaagaattgg aagaaatcca agcgcaagta aaggaagcag tagaagcttc tgttaaattt
     5281 gcagaggaaa gcccattccc accgcttgaa tcagcctttg aagatattta cgcagactaa
     5341 ggagaaagag ataaaatgga aacaaaaaca atgtccttcc gtgacaccat tatccttgct
     5401 atgtctgagg aaatgcgtcg cgatgaaaat gtgttcttga tgggagaaga tgtcggtgtc
     5461 ttcggaggag acttcggtac ttctgttgga atgcttgaag aatttggtcc agaacgtgtc
     5521 cgtgactgtc cgatttctga agctgccatc tctggagcag cagcaggagc agccatgaca
     5581 ggacttcgtc caatcgtcga tatgaccttc atggacttct ctgttattgc catggacaat
     5641 atcgtcaacc aagctgctaa aacacgttac atgtttggtg gtaaaggtca ggttccaatg
     5701 actgttcgtt gtgcagctgg taacggagtt ggctctgccg cccagcactc acaatctcta
     5761 gagtcttggt ttactcacat tccaggactt aaggttgtgg cacctggaac acctgcggac
     5821 atgaaaggcc tgcttaagtc ttctatccgt gataacaacc cagttattat acttgagtac
     5881 aagtcagaat ttaaccaaaa aggggaagtg ccagttgatc cagactacac aattccactt
     5941 ggggttggtg aaatcaaacg ccaaggaacg gatgtaacag ttgttactta tggaaaaatg
     6001 cttcgccgtg tggttcaagc tgctgaagaa ttagcagaag aagaaatttc agttgaaatt
     6061 gttgacccac gtacccttgt tccgcttgat aaggatatca tcataaactc agtgaagaag
     6121 actggtaagg ttattctggt caacgatgcc cacaaaacaa gtggctatat cggtgaaatt
     6181 tcagctatta tttcagaatc agaagcattt gactatctag atgcaccaat acgccgttgt
     6241 tcaggagaag atgtgccgat gccttatgca caaaacctag aaaatgcaat gattccaaca
     6301 gttgaaagta tcaaagatgc aatccgaaaa acttataaca aagactagag ttacataata
     6361 taagttatgt aactaaaata aagctgacga gaaactttgt atcttttagg tgcagagctc
     6421 ttcgtcatta atatcttata ttcgagcacg ggctaaaagc tcggaaaaaa gataaatctc
     6481 cttggcttca tcgaagccat cgtcgatttc ctatttttca gtcgcttttg acgccctttg
     6541 tatcataaat agaattggag aggtcatggc tgatgacaag ctaagagcga ctcctgcagc
     6601 tagaaagtta gcggatgatt tagggataaa cctctacgac gtttctggct caggtgcaaa
     6661 cggtcgtgtc cacaaagaag acgtggaaac ttataaagac acaaacgtgg ttcgcatttc
     6721 gccacttgca aaacgaattg ccctcgaaca taacattgct tggcaggaaa tccaaggaac
     6781 cggtcatcgt ggtaaaatca tgaagaagga tgttttggcc ctgcttcctg aaaatatcga
     6841 aaacgatagc atcaaatctc ctgctcagat tgaaaaagtg gaagaagtcc ctgataacgt
     6901 aacaccatat ggtgaaatcg agcgtattcc aatgacacca atgcgtaagg ttattgccca
     6961 acgcatggtt gaatcttact tgactgcgcc aaccttcacc ctcaactatg aagttgatat
     7021 gactgaaatg ttggctcttc gtaagaaggt tcttgagcca atcatggaag caactgggaa
     7081 gaagactact gtaacagacc ttctttcgct tgcagttgtt aagactctta tgaaacactc
     7141 atacatcaac gcttcattga cagaagatgg caagactatt atcactcaca actatgtcaa
     7201 tcttgccatg gcagttggga tggataatgg attgatgaca cctgttgttt acaatgctga
     7261 gaagatgagt ctttcagaac tggtggtagc ctttaaggat gttattggcc gtaccttgga
     7321 tggtaaattg gctccaagtg agttacaaaa ttcaacattc acaatcagta atttgggaat
     7381 gtttggtgtt cagtcctttg gtccgattat taaccaaccc aactcagcta tccttggtgt
     7441 cagttcgaca atcgagaagc cagttgtcgt caatggtgaa attgtgattc gcccaatcat
     7501 gagtttagga ttaaccattg accaccgtgt cgtagatggt atggctggtg ctaagtttat
     7561 gaaggacttg aaagagttga ttgaaacgcc aatctcaatg ttaatttaag agcaagagta
     7621 ttcattttta agatatagat aaaggaagga agacatggcc ttagaaataa ttatgccaaa
     7681 agccggcgtg gatatgacag aaggacaaat cgtccaatgg aataaaaaag tcggagaatt
     7741 tgtaaaagaa ggagaaatcc ttttggaaat catgactgat aaagtcagca tggaattgga
     7801 agccgaagaa gatgggtact tgattgccat tctcaaagga gatggtgaaa ctgtccctgt
     7861 aacggaagtt atcggttacc ttggtgaaga aagggaaaac atcccaacag ctggagcagc
     7921 atcgccagaa gctagccctg tacctgtagc aagtacctca aacgatgatg gtaagagcga
     7981 tgatgctttt gatatcgttg tgattggtgg aggtcctgct ggttatgttg cagccattaa
     8041 agctgcccaa ctcggcggta aggttgccct tgttgagaaa tctgaacttg gtggaacctg
     8101 cttaaaccgt ggatgtattc caaccaagac ctaccttcat agcgctgaaa ttattgaaaa
     8161 tatcggtcat gctgcaaatc gtggtatcgt gattgaaaat cctaacttca ctgttgatat
     8221 ggaaaaactt ttagaaacta aatctaaagt tgttaatact cttgttggtg gtgttgcagg
     8281 acttcttcgt agctacggag ttgctgttca taaaggaatt ggtacaatca ctaaagacaa
     8341 gaacgtcttg gtaaatggtt ctgaattgct tgaaaccaag aaaatcattc ttgctggtgg
     8401 ttcaaaagtc agcaagatca acgtccctgg tatggaatct ccacttgtca tgactagtga
     8461 tgacattctt gaaatgaacg aagtgccaga aagccttgtt atcatcggtg gtggagttgt
     8521 cggtatcgaa ctcggtcagg ccttcatgac atttggttca aaagtgactg ttatcgaaat
     8581 gatggaccgt atagttccag ctatggatgt ggaagtttct aagaaccttc gcttgatcct
     8641 tgagcgtaaa ggaatgacca tcttgactgg tactaaactg caagaaatca ttgaggaaaa
     8701 tggtcaactt cgtatcaagg ttgaaggaaa agacgatatc atcgcaagca aagctcttct
     8761 ttcaattggt cgtatgccag accttgaagg tattggagag gttgagtttg aattggatcg
     8821 tggttgtatc aaggtcaacg aatacatgga aacttcagtt ccaggcattt atgcaccagg
     8881 tgacatcaac ggtactaaga tgttggctca cgcagctttc cgcatgggtg aagtttccgc
     8941 tgaaaatgcc cttaaaggaa atcatgcagt tgccaaattg aatttgactc ctgcagccat
     9001 ctacactctt cctgaagtag cagcagtagg tttgacagaa gaacaagccc gtgagaaata
     9061 cgatgttgcc atcggtaagt ttaactttgc tgctaacggt cgtgctattg catctgacgc
     9121 agcccaaggt ttcgtaaaag ttatcgctga taagaaatac ggagaaatcc ttggtgtaca
     9181 cattattggt cctgcagccg cagaattaat aaacgaagca tcaagcatca tcgaaatgga
     9241 aatcactgtt gaggaaatgc tgaagaccat ccacgggcac ccaacttact ctgaagtgat
     9301 gtacgaagcg tttgcagatg ttctaggaat ggccatccat tcacctaaga aaaaataaaa
     9361 cgaagataga ttagactgga aggttatttc cagtttctat catataaagt aaagggatat
     9421 catgaaatac attatcaatc attcaaatga cactgctttt aatattgcct tggaagaata
     9481 cgcctttaaa caccttttgg atgaggatca aatcttccta ctttggatta acaagccatc
     9541 tatcattgtt ggtcgtcacc agaacactat tgaagaaatc aaccgtgatt atgttcgcga
     9601 aaatggtatt gaggtagttc gccgtattag tggtggtgga gctgtttacc acgatttaaa
     9661 taacctcaac tacacgatca tctccaaaga agatgaaaat aaggcatttg acttcaagag
     9721 cttctcaact ccggttatca ataccttggc tcaactcggg gttaaagctg agttcacagg
     9781 tcgtaatgac cttgagattg atggtaagaa gttctgtggc aatgcccaag cctatatcaa
     9841 cggccgtatc atgcaccacg gttgcttgct ctttgacgtt gatttgtcag tcctcgcaaa
     9901 tgcccttaag gtttcaaaag ataaatttga atcaaaaggt gtgaaatccg tccgtgcccg
     9961 tgtaaccaat attatcaatg aattaccaaa aaaaatcaca gtcgaaaaat tccgtgattt
    10021 actcttggaa tacatgaaaa aagagtaccc agagatgact gaatacgtct tttcagaaga
    10081 agaattggcc gaaatcaatc gcatcaagga tactaagttt ggaacttggg actggaacta
    10141 tggtaaatca cctgaattta acgtccgtcg tggaaccaaa ttcactagtg gtaaggttga
    10201 agtctttgct aacgttactg aatcaaaaat ccaagacatc aagatttatg gtgacttctt
    10261 tggtattgaa gacgttgctg cagtagaagc tgtccttcgt ggggtgaaat acgaacgcga
    10321 agatgttctt aaggcgctaa aaaccattga tatcacacgc tacttcgctg gtattagccg
    10381 tgaagaaatc gctgaagcgg tagttggata aatcaaaaag aagttggcca actaaccaac
    10441 ttcttttttt gatatttatt tacataattt aaaatacgta aaatcataaa ctatccagag
    10501 cattcttttg ttcatcacta acaatatggg tatagaggtc agtgacttgt gtgctagcat
    10561 gtcctagttg gtgactgact aaaacttgtg atttagtcgc atcatagagc ctagttgcta
    10621 gtgtatggcg cagtttatgg ggtgttacac gcactttaaa atcctctgag tatttagcaa
    10681 ccattttctc aacgctagaa gcatcgatac gattaggaac acctctgtag agagttaaaa
    10741 aaagggctgt atctgttttt tccgttttat agcgttgatt ccgaatggcc agataattct
    10801 ctaaataagg tttagcaaaa gcagcgacat tgactgagtc acgtttgcca ccttttcgag
    10861 taacatcaat aaccatcatt tttagattga gatctcttag atctagatta acagcttcag
    10921 ataagcgaac accagatgcc aagagaaggg caataatggc taaatctcgt tctttatttt
    10981 tgttgaatga tgagagagct cgatttgaaa gctgttgtgg gtgctcttga tcgatataag
    11041 ttagaaaacc ttctgtttca tcacctagaa agagtttttg cttgatattt tcagctctgg
    11101 cagcaagggt ttctttcttt ttcttggttg aaactttttt cattacatta cgatagaaat
    11161 aaggttcccc ctgatcgttt tcaacctcct cggttagata cttgtaaaga ctagaaagtg
    11221 ctgataaggt tcgattgata gttgtctgtg aaacaccttg ttttgttgta ttagcattca
    11281 gcaagggacg ttcacgtaga taaaggataa aggattccat gtctttctta gacatatttt
    11341 ccaaaactga taaaggaata tcggatattt tatcagcgtt tgaaatacca gactccaaaa
    11401 cccagctgaa aaatcggtca tattccttaa ggtattcgta cagggttgta aaactgtagg
    11461 gcacagccag tttagattgg tagtattcca gaacatacca gggcatgagt tgttttagtt
    11521 tgtcgattcg ttccagtaaa atctcacgtt tcatgtattt tctccataat taagtatact
    11581 agtagtatag catagacttg tatatttttc aagaaaatta tagtttttct gaaagatgtt
    11641 ataggagttt ttcaaagtga taaaaagact tagacctaca aacaaaacag aataaggaaa
    11701 ataataaaaa tctcacccac taaacttctg ttaatactac ataactgaag agctatataa
    11761 ggagctatga gatacaaaac acattgtata atagattgaa actagaatag tacatctgga
    11821 cttctaaaac attgctagaa attgatttga ttgtcctgat tgttttatcc tatttctatt
    11881 tcatttctct atataattgc tattgttttc tttttcatga ttttaatctt actgtacaga
    11941 tttgatacag taaactcctt tctagcttta tactttatca tttctaactt aattatagtc
    12001 ttattttt
//
LOCUS       DAVKLX010000060        11374 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKLX010000060 DAVKLX010000000
VERSION     DAVKLX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.060"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(218..514)
                     /locus_tag="VW325_001839"
     CDS             complement(218..514)
                     /locus_tag="VW325_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEU9735181.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            complement(1045..1938)
                     /locus_tag="VW325_001840"
                     /pseudo
     CDS             complement(1045..1938)
                     /locus_tag="VW325_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(2692..2922)
                     /gene="blpU"
                     /locus_tag="VW325_001841"
     CDS             complement(2692..2922)
                     /gene="blpU"
                     /locus_tag="VW325_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU9735182.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCWW"
     gene            complement(3125..3415)
                     /locus_tag="VW325_001842"
                     /pseudo
     CDS             complement(3125..3415)
                     /locus_tag="VW325_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3461..3694)
                     /locus_tag="VW325_001843"
     CDS             complement(3461..3694)
                     /locus_tag="VW325_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9735183.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(3700..4692)
                     /gene="plsX"
                     /locus_tag="VW325_001844"
     CDS             complement(3700..4692)
                     /gene="plsX"
                     /locus_tag="VW325_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9735184.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(4689..5459)
                     /gene="recO"
                     /locus_tag="VW325_001845"
     CDS             complement(4689..5459)
                     /gene="recO"
                     /locus_tag="VW325_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9735185.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDDRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(5456..6625)
                     /locus_tag="VW325_001846"
     CDS             complement(5456..6625)
                     /locus_tag="VW325_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9735186.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            6774..7784
                     /locus_tag="VW325_001847"
     CDS             6774..7784
                     /locus_tag="VW325_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEU9735187.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSIVLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            complement(8067..8504)
                     /locus_tag="VW325_001848"
     CDS             complement(8067..8504)
                     /locus_tag="VW325_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9735188.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(8589..11222)
                     /gene="polA"
                     /locus_tag="VW325_001849"
     CDS             complement(8589..11222)
                     /gene="polA"
                     /locus_tag="VW325_001849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9735189.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEYGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAKGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCEDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPDIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
BASE COUNT         3334 a         2717 c         1863 g         3460 t
ORIGIN      
        1 tttttttact ttggataggc agtttcatat tataacgatt caaagtagcg taatctacta
       61 ccaaaaaaag taaaaataat ttcccttgaa aaggaagata gcttttaaaa tagattgaca
      121 aacttatgat aacaaaaaac aaaagggtta tcccaataaa attaagataa cctaaaatac
      181 tttcacattc ttttttagaa agtattttca tctagttcta ccatagagct gctttagcag
      241 cacctttctt tttcaatata taaagaactg ttgctgcaat acccgaacca atagaaccta
      301 ctcctgtgat tgcacctaca attgaaatta ctgctgtaac tgtactataa gcactaatca
      361 aattgactac agtagcagca gcttttgtcg aaattcctaa tgtcccagcc aacataggaa
      421 aagaaacaac acctaagctt aaccaactaa tgactgctaa cattgctgaa acaataagaa
      481 atttattatg aaattccatt cttttagatt tcattttagt gtactccttt tgtatattat
      541 gtatgtttta ttgagagtta attcgcacat gttgtattcc taactctctt caaatgttcg
      601 tctatatttt atactacttt tattaacata tctataataa ataaattact actacatttt
      661 cctagattat caaattactt ttatatcact aagactcaat aaaagataat gtctatttaa
      721 cttttatacg attactacct tctttttagt ttacacttcc tacatcataa ataaacatac
      781 tcaaccctca caatctccaa ataactcttc tatatcattg aaaattgagt ttcatcctct
      841 atactaatgt aggacattcc tttaatccaa tataagaata ccttgttcca ttaatctcta
      901 cccgatatta tagatagaac caaattccta tacttaaaag gacaaataac gttccaacca
      961 taataaacct cctttctttt tgagaacgtt ttctttatag gtttagtata gcaaaaacat
     1021 gaaaaaaaca acatgactta gaagtcaggt tgttgggaaa tctatctgtt tttctgctaa
     1081 aattctctga actaatacct gatcgtctag caattctgct agagaaatcg ccttttcata
     1141 gaaactttct gcctctaaaa tattcttgtc tacaaataaa gcatattttg cctttagcaa
     1201 aaatagatga ggtcggtaaa aaacatgtct tgttttagca ataattttct caaatgtttg
     1261 aatatatatc agactatctt ctgtctgaat ttggataaat aggctcagaa gaactttttc
     1321 catttgaact aggctagcct tatcttcata ttctgaacat agcaatactt tttgagctaa
     1381 ctcctcaaaa tgttctttat cttctaatcc catagcacaa caaaagaaat acaattcaat
     1441 aatcaacaaa tcatttacag aaaaatattt cttttttaat aactgctgga aatattcttc
     1501 cactaaccct aaaccgtagg tcacatctga actaccataa atatcaaaac gagcttgtgt
     1561 cacatccaca attaactgct cttcctcagg caaattctca taatagtttt catatatttc
     1621 ctcaagtata gcttcttttc attcgattct tcccttatca gcgtaggttt gagaatgaat
     1681 cagctcatct ttgagtttta ggaatccttt tggaatctct ataaccttaa catccacaat
     1741 attctgtaat gatactccaa gtttatccgc aatcaataat aatttaggaa gtgttgctaa
     1801 agcttgccca ttttcaatac gtgctagctg acgaatcgtc aactctgatt catcaccaca
     1861 aatatctatc tgagtttttc cacaagatcg cctaatagtt ttgattttat aaccaatttc
     1921 acgtttcaac tcatccatag taatactcct taagttgatt acaaagattt ctttttcttc
     1981 ctaattcttt tgtcaatcat atataaaacg ataagcgaaa acagcggtag aacaatataa
     2041 ctcttttcac tcattatcag ctgaaatccc agggttgcta gtatcttaaa gatgaaaata
     2101 ttcatcaaaa atagaaaaac aaatactaaa aaatatttta aaaccttctt catttctttt
     2161 cttcaacttt cctaatttct acacctattt tatcgaaaat atatataaaa ctccaaataa
     2221 acacttcaac tgcacttttt ttggcaaaaa atagacagaa caaacatcct acctactaaa
     2281 aatatgatat gaaaggaaaa tgatttttta ctgacaccct aggcaagctt gggacaaata
     2341 aatcattgat agcaacattt acgacatcct atttaaagtt ccttcaattt ctaatgtccc
     2401 cgctatttct aagccacctc tggtatgatc tattcctagt cagaatagat aacaccgttc
     2461 taaattacga ttcatagtta ctcctttttt gactcgttac tataagactc ctatttacat
     2521 gcgcaaaata attttgattc agattcatca tttatagttt tgattatttt ttaacagatt
     2581 tttctgtttt cttctccgaa gactttaaaa atttttttct aattaaatca tccatataag
     2641 gaccaaatat accaactact aaaccaataa taaaactttt aaaatccata attaccacca
     2701 acatgtcgct gcataggcca cacctccaag tatagctcct cccacagcac cagttgctgc
     2761 accttgccat gttcttgttt taattcctag ctgaagacct cgtgctgctc ctcctccaac
     2821 acctgctttg gcaaaatctc cccaattgca tccgccacct tcaacgcaag caagcatctc
     2881 agtatccata atttcaaatt gtgacatcgt ttttgtattc ataacgaata cctctttttt
     2941 atttttaata tttgtcttgt tacaaacttg acaagtttag tataacagta tctattaatt
     3001 tttttcatcc aaatcttgaa ttggcatcga aacgtcttga attagctttt ttgtttcaaa
     3061 atcatctcta tttttaaaaa agatgttttc taatcacttt tttactatta gacttcctgc
     3121 aaaactagaa tcctagttca tgattgataa taccagcaat caaattcatt cgtaatccga
     3181 agcgtttacg atgatttcga taggttgttg aaaacatttt aaacgttttt actttggcaa
     3241 agatattctc aaccttgctt ctctccttag atagcgcatg attacaggct ttatcttcaa
     3301 ctgttagcgg cttgagtttg ctggatttac gtggagtttg tgcttgagga tatatcttca
     3361 tgagcccttg ataaccactg tcagccaaga tttttccagc ttgtccgata tttctcattt
     3421 ctaaaaacca tctacttccg ttgacttgga actccgattg ctattttcct tgaatgattt
     3481 taaccacatc tcctacgttt tggagttggt caatttcttc atcgctgatt tcgatactaa
     3541 attcatcttc cagagtcaag ataaactcca tcaagtcaac agaatccgca tccaaatcgt
     3601 ctttcagact caaggattct gtcacgacaa agtcctctcc ctgtcgctct tggataatgg
     3661 tcacaatacg gtcaaaaatt tctttttctg tcatctcttt tattctcctg aaaattcacg
     3721 cgcagtctgg gcaaccacgt ctgtttctag catggtacgg atctgacgaa tcgtactata
     3781 aacagccttg gcatcgcttg agccatgagt cttgacaaca ggtgccttaa caccaaacaa
     3841 gaccgctcca ccaacatctg aataattgag ctgttttttc aaaccactga ggctgtcctt
     3901 gagaaggagg gcacctagtt tcgctcgaag accaccacct gtaatagctg tcttgagcaa
     3961 gcccatgatt cccatagctg tcccttcgat ggatttgagc acagcgtttc ccgtgaaacc
     4021 atctgccaca acaacatctg caacgccatt catcaaatca cgcgcttcca cgtttccgat
     4081 aaagttcaaa ctttcatcag ccgccagtaa ttcataagtt tccttacgaa gcgggtcgcc
     4141 cttgctactc tctgttccgt tgttgagcaa accaacgcgt ggttgcgcaa tgccacggac
     4201 atttttagca tagaaggaac ctagaaccgc atattgatgg aggtgctggg ctgtattttc
     4261 tgcattggca ccaaggtcta gcatgtcaaa accttttcca tcaacggtag gcaaggtaga
     4321 catgagtcca ggacggtcga tattcttgat acgacccacg atgaagaatc ctgctgccaa
     4381 caaggcacct gtattcccag ccgaaaggac agcgtctgct tcaccatctt tgacagcctt
     4441 ggctgccaat accatactgg catttttctt attccgaata gctctcgtag gttcatcatc
     4501 cgaatcaatc ttctcatccg tatggataat gctgacgcgc tctgtcgctg tcagatattg
     4561 cttgatttta gcttcatctc cgtaaagttg aacctcgata tctgaaaagt cagatagggc
     4621 ttgattgaca ccctcaacga tggcctgagg tgcgtaatcg ccccccatgg catctactgc
     4681 gatttttttc atttcttttc ctcttttagt aattgtcccc agtctgctag ggaatcaata
     4741 aatttctttg attttaggtg aatcccaacg tactcttcat atagttgatc cataaattgg
     4801 cgtagctctt gcttgattcc aggcttgagc gaaatggtct ccaaagtctc aaaatcaata
     4861 gcttgaaatt gattgagcag ataggggata ttgggattga gatggcaacg tctatcatcc
     4921 tcatgataat gctctggaca gagacaggct ccatatttga aagaaaagtc aaaagcctga
     4981 ccaacccgat ggcagaagac acactcatta aaattgaggc tgattccaaa tcgagtcaaa
     5041 atttgaattt caaaaatatt ggtcaaaacc tgataatcca agcctgcttc catcaactcc
     5101 aaagtctttt gcaaaaaagc aaacaaggga gcatcctgct gattgtcctg caaactagca
     5161 tctgcaagag ctgccacata ggtcgcatag gccatgacaa agaggtcact attaatcttg
     5221 ggaaaagtca tgacctcatg atagtcttcg atgtaactga gtccgtcatc attgattcgc
     5281 aagagaaatc gtgccagcac caagggctga ataacaggcg ccagcttaga ctgaccagcg
     5341 tgtttgacaa aaaacatgcg tttgccaacc tgctctgtaa aaattttgac gagcttgtca
     5401 tcctcacgaa aattgcgatt gtaaagcacc aagccttgac tcgtgataga ctgaatcatg
     5461 cttctctcat gtactcctca agtcgtttca tggcttcttt gatagtctcc atgctggctg
     5521 cataagatag gcggacgtag ccttccccgt aacgtccaaa ggctgcacca gggataaagg
     5581 caacggcctt cttctgagca aaatccttca gaaaagcaaa ggagtcttga ttgtagcccg
     5641 ctggaatttt agcaaaaata tagaaggcac cgtctggttt gataatctca aaaccaagag
     5701 cagtcatttt ttcgatgata tagtcccgac gctggatata ttccttcttc atgggctccg
     5761 catcgttttt accagccgtc aaggcttcta ctgcagcatg ttgcgccatg gtatttgcgg
     5821 cagtgaccaa gtactggtga ctcttgatta actgggctgt gaaggtcgca ggagcgaaaa
     5881 tcagccccaa acgccaacct gtcatggcat gcgatttaga caaaccattg ataataatag
     5941 cctggtctct caacatcgtt cctagagaca catgggcttc gcctgtgtag gtcaattctg
     6001 agtaaacctc atcacagaca acaaaaattt cgtacttgcg taaaacagct gccaaggcct
     6061 ctaactgctc tcgactgtag gtaattcctg tcggattggc tggatagttg agaataaccg
     6121 ccttgagctt atcaccctgc tccaaaatgg ccttctccaa catctcagga gtcaagacaa
     6181 aaccattttc agtcgtatca atctcaacaa tttctgcccc aactaagtta acaatcggtt
     6241 catagcctgg ataagcagga gctggcaaaa gtaccttgtc tccctcttcc aaaatagccg
     6301 tcaaagtcgc agataaagcc tctgtcgccc caattgtaac caagatttca ttttcaggag
     6361 catagtccag ttggtacttt tccttaacaa agtcactggc tgcctgacgt agagtcagca
     6421 gaccactcat ccctgtatag taggattggt tctgatcaat cgctcgcttg gccgcctcct
     6481 tgacatggtc tggcgttgta aaatcaggtt cccccaaggt caaacgcaag accccaggaa
     6541 tctccgaaat aacctggtca aactgacgaa tcaacgaaac ttgaatttta tctaactgtt
     6601 tattaaagcg cttagttaag tccatagata cctcctactt tcaaaacgta tctctattat
     6661 actacaaaag ccccccgacc gcaaaaatac attagagaat agctttccac tttctatttt
     6721 acttttcctg tttataggtc tataatggta atagatttta tttggaggtt tttatgtcat
     6781 ttctatcaaa aaatggagca ggtatcttgg cctgccttct catttccatc ctatcttggt
     6841 acttaggagg attcttccct gtgattggcg cgcccgtttt tgccattttc ataggcatgc
     6901 tcctacatcc ctttctctcg tcctataaac aactggatgc tggtttgacc tttagttcca
     6961 agaagttgct ccaatatgcc gttgtcttgc ttggttttgg tctcaatatc tcgcaggtct
     7021 tcgcagttgg ccaatcttca ctccctgtca tcctgtccac tatctcaata gctctgatta
     7081 ttgcctacct cttccagcgt ttctttgcac tggatacaaa actggctacc ttggttggag
     7141 taggttcttc tatctgtggg ggttctgcca ttgcagcgac agcgcctgtt atccatgcta
     7201 aggaaaagga agtagcccaa gccatttccg ttatcttttt cttcaatgtc ttggctgcgc
     7261 tcatctttcc aaccctcggc acctggcttc atctatccaa tgaaggcttc gccctctttg
     7321 cagggactgc ggtcaacgac acttcctctg taacggctgc cgccagcgct tgggacagtc
     7381 tttaccaaag caataccctc gagtctgcaa ccattgttaa actcacacgt actttggcca
     7441 ttatccctat cacgctcttt ctatcctact ggcaaagtcg ccaacaagaa aacaagcaaa
     7501 gcctacaact gaaaaaagtc ttcccacttt ttatccttta ctttatcctt gcctctctcc
     7561 tcactacact actcacctct ctaggtgtgt ccagtagttt ctttacccct ctcaaacaac
     7621 tctctaaatt ccttattgtc atggccatga gtgctatcgg tctcaaaacc aatctggtcg
     7681 ctatggtcaa atccagtgga aaatccattg ttcttggagc catctgctgg attgccatca
     7741 tcctcaccac tcttggtatg cagaccctta tcggcatttt ctaacactct tcgaaaatct
     7801 cttcaaacca cgtcaacgtc gccttgccgt aggtatatgt tactgacttc gtcagttcta
     7861 tctacaacct caaaacggtg ttttgagctg acttcgtcag ttctatctac aacctcaaaa
     7921 cggtgttttg agctgacttc gtcagttcta tctacaacct caaaacggtg ttttgagcag
     7981 cccgcggcta gtttcctagt ttgctagtgt gctctttgat tttcattgag tataacacaa
     8041 aaggtagccc atcagctacc tttttcttat gcttcctcaa tcaagcgagt atgttctctc
     8101 ttgatacagc gattcatcac gatatcatca catccaccat cacgcaaaat ctctttcgct
     8161 tctaaacttt caagtcctag ctgtgcccaa aaaatcttgg catcagcttt gagaaaatca
     8221 cgcgccacat cgggcagaaa ttcactgcga cgataaacat tgacaatatc tacaggaaaa
     8281 ggaatttcag cgaggctagc ataagccttt tcacccaaga tttcgccacc tgccgccttg
     8341 ggattgactg ggatgatttt atagccccga gcctgcattt cctttgttac tcgattgctg
     8401 gttgtttctt cacggtcaga caaacccacc acagcaaggg ttttactcgt tgcgagatac
     8461 tgacgaatca cgccatcact tggattgata aattcttgac tcatagaaat cctccttttt
     8521 catcagtata gcacattttg aaaaggtttg cagaattata ctacaaaaaa ggaggactag
     8581 cccccttttt atttagcctc gtaccaggtt gccccttcat tctcatctgc gataagagga
     8641 acactgagtt gaatggcttc ttccatggtt tgtttcacca attttttcat ctctaccaat
     8701 tcagatttag gcacttcaag gacgatttca tcgtgcactt gtaacagcat cttagtctga
     8761 taaccacctg caaccaaggc tttatccagc tgaatcatgg caatcttgag aatatctgct
     8821 gccgaaccct ggataggtga gttgatagca gttcgctccg caaaaccacg aatattgaag
     8881 ttgcgcgaat tgatatctgg caactcacgg cgacgcttaa agagggtctc tacatagccc
     8941 ttatcacgcg cctcccgcac cacttcatcc atgtagtttt taatatctgg aaaacgttca
     9001 aagtaggtat caatgtaggc tttggcttcc ttacgactaa ttcccaaatt attagacaag
     9061 ccaaagtctg aaatcccata aaccactcca aagttaactg ccttggcatt gcgacggtca
     9121 tttgcagtca cattatcagg acgctcaatg ccaaagaccc gcatggctgt cgaagtatgg
     9181 atatctgccc cctcttggaa ggccttaatc aagtgctcat ccttagaaat atgcgccaaa
     9241 acgcgcaatt caatctgtga atagtcagag ctgagtagca cactatcctc ccactctggc
     9301 acaaaagcct tccgaatcaa gcgcccttgt tccaatcggg caggaatatt ttgcaagttt
     9361 ggatccacac tagacaaacg cccggtctgg gtcaaatcct gcatatagcg agtatgaatc
     9421 tttccatcag ccaaaatcca gtcctgcaag ccaattacat aagtagattg aatcttagca
     9481 atttgacggt aatccaggat tttcttaaca atcggagcaa taggagcgag acgctccaac
     9541 acatccactg ctgtcgaata acctgtcttg gttttcttag tgtattctag aggaagtccc
     9601 aatttctcaa agagaagcac gcccaactgc ttaggcgagt tgacattaaa ctcctcacca
     9661 gccagctcgt aaatctcttg agtcagtttt tcaatgacaa gctcattttc agcctgcatc
     9721 tcaagcaagg tctctttctt gaccacaatc ccagcaattt ccatcttggc aagaacaaaa
     9781 gccagaggtt gctccatatc ataaagaagc tctaattgcc cattttcgct gagtttttca
     9841 agtaaaatag gctctgtttc taccaaaaca gcaagtttac aagctaagtg ttccaagaat
     9901 ttctcacgtt caggaatggc ctttttaaca cccttaccgt agaaagtttc atcatcaacc
     9961 aagtaagtct gaccataaag actagcgatg gtcgcaattt cattgtcctc cacagtcgaa
    10021 aggaggtatt tagccaaacg gatgtcaaaa gcaggcgcct gcaaatccac accaaaacgt
    10081 tgcaaaagaa ctttaacctt cttaaagtca taaactctca gagatgtttt ttctaagaaa
    10141 tccttgaaaa tcgggtcttg caacagctca agcttgtctg tggcatagag cttatcctca
    10201 caagaccagg caaatccaac caaattatcc gtatggtaat tctcaccaaa aagctcaaag
    10261 tggaagatag actcttcact cagcatatct tgactgattt ggtcaacaat agtaaaatcc
    10321 aaacccttag ccacatcagc tgacgacaca tttaaagcct gcttgagctg tttgaatccc
    10381 atctcatcgt aaaatttccc aagattttca acatctggac cactatagac caagtcctct
    10441 aaaccaatcg caatcggtgc cttggtatca atggtcgcta gtgttttaga caaaaaggcc
    10501 tgttccttgt cattgatgag attttccttc atcttagaag tcttcattcc atcaatattt
    10561 tcataaatcc cctcaagcga accatactcc agcaagagct taatacccgt cttttcaccg
    10621 actttggtca ccccagggat attatccgac ttatcaccca tgagcgcctt gagatcgata
    10681 aactgagctg gtgtgaggcc catttcttcc atgaggtaat ctggcgtaaa ggcctcaaac
    10741 tcagccacac ctttcttgga aatttcaacc accgtatgct catccgtcag ctgaatcaaa
    10801 tccttgtccc cactgacaat ggtaatatca aaaccatcct gctctgctag cttatccagc
    10861 gtcccaatga tgtcatccgc ctcatactga gccagctcat agtgacgaat ccccatatga
    10921 tccagcaact cacgaatgaa aggaaattgc tcacgaaact catcaggagt cttggcccga
    10981 ccacccttat agtccgcata catctctgtc cggaaggtcg tctttcccgc atcaaaagcc
    11041 accaaaatat gactcggctc aacccgctcc aacaaatgac tcaacatcaa ctgaaaacca
    11101 taaatcgcat tggtatgcaa accagccgca ttcttaaaac ggtccaactg ctgatacagc
    11161 gcaaaaaacg cccgaaaagc tacagaagac ccatcaatca ataataattt tttcttatcc
    11221 atacacccat tataaaggaa agaatcaaaa aataccattg ggaagagcta gagcaagtat
    11281 ttttcaaact ttttccgaat aaatagatag agccagagaa tttagtaaac ctagatttaa
    11341 aaatgtgcta taatataata tattgaatct ataa
//
LOCUS       DAVKLX010000061        11051 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKLX010000061 DAVKLX010000000
VERSION     DAVKLX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.061"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            99..461
                     /locus_tag="VW325_001850"
     CDS             99..461
                     /locus_tag="VW325_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735190.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGRILA
                     IIGGSLFLATLKKFKSEE"
     gene            996..1688
                     /locus_tag="VW325_001851"
     CDS             996..1688
                     /locus_tag="VW325_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9735191.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1741..4128
                     /locus_tag="VW325_001852"
     CDS             1741..4128
                     /locus_tag="VW325_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9735192.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GSERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNSDYHLNVNLQMNY
                     WPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHTQATPFGWT
                     APGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFWNAFLHKDQ
                     QAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLLTEVKEKSD
                     LLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSYKGQEYIEA
                     ARASLNDRGDGGTGWSEANKINLWARLGDGNRAHKLLAEQLKTSTLQNLWCSHPPFQI
                     DGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSMSWEDKKLL
                     QLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDLVQFYF"
     gene            4378..5079
                     /locus_tag="VW325_001853"
     CDS             4378..5079
                     /locus_tag="VW325_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9735193.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNSDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5081..7789
                     /locus_tag="VW325_001854"
     CDS             5081..7789
                     /locus_tag="VW325_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9735194.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEAKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSLLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7885..8376)
                     /gene="tpx"
                     /locus_tag="VW325_001855"
     CDS             complement(7885..8376)
                     /gene="tpx"
                     /locus_tag="VW325_001855"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9735195.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGHDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8499..9428)
                     /gene="psaA"
                     /locus_tag="VW325_001856"
     CDS             complement(8499..9428)
                     /gene="psaA"
                     /locus_tag="VW325_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9735196.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9460..10308)
                     /locus_tag="VW325_001857"
     CDS             complement(9460..10308)
                     /locus_tag="VW325_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9735197.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10305..11027)
                     /locus_tag="VW325_001858"
     CDS             complement(10305..11027)
                     /locus_tag="VW325_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9735198.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3233 a         2057 c         2521 g         3240 t
ORIGIN      
        1 tatacaaatt tttattttat aatatcagaa aatacttttt ttaaaaagca aatatgatac
       61 aattttattt gaaaaaaaat aaaaaaggag attttattat gaaatcaagt ggtatcgtcg
      121 gtcttgtggg aggaatttta cttcttattt gtccttttgt cttgttggga acagcggtaa
      181 atacagctgc tacaactctt aatggaggag ctactgcagg ggctttttca ggtgttgcct
      241 tactcttgaa tgccttgaag attgcaaatc ttgttcttgg tatcattgct attgtttact
      301 ataaaggaga taagcatgta ggtgcagctc cgtctgtact aatgattgtt tctggtgaag
      361 ttagtctcat tccattctta ggatgggttg ggaggattct tgctattatc ggaggatctc
      421 tattccttgc aacattgaag aaattcaaat cagaagaata aaaggtattt tagcatgaaa
      481 agaacaaaaa agtttatcgg tataggagta gctctattat ctctttctct tctagttgca
      541 tgtggaacat aaagttcaaa gaatacttca acaagtaatg atgagaagac agtagcaaca
      601 tccaatagtt caaaagaaac aatcactttc gatacaccgg ttgtaacaga cgatgcgatt
      661 gaatcaatac gcacttatgc agattatata gatctttata aaaatatttt tgatgattat
      721 tttactaaag ctgaggaagg tttcaaaggc acaactatgg aaaataatga ctcgtttact
      781 aaactaaaag agtcaactca aaaattattc gatgcgcaga aaaaaaggtt aaataatgaa
      841 gatagaatag aaacaaccaa aaacaatgtg attgccaaac attgtcaaac agtcctttcc
      901 tttttggttt tgactagctt ttttgtgaaa aattgtgtaa aatagaatag ataaacgagg
      961 ggaaacctcg gaaaatttaa aggagaatcc atctaatggt aaaattggtt tttgctcgcc
     1021 acggtgagtc tgaatggaac aaagctaacc ttttcactgg ttgggctgat gttgatttgt
     1081 ctgaaaaagg tacacaacaa gcgattgacg ctggtaaatt gatcaaagaa gctggtatcg
     1141 aatttgacca agcttacact tcagtattga aacgtgctat caaaacaact aacttggctc
     1201 ttgaagcttc tgaccaatta tgggttccag ttgaaaaatc atggcgcttg aacgaacgtc
     1261 actacggtgg tttgactggt aaaaacaaag ctgaagctgc tgaacaattt ggtgatgagc
     1321 aagttcacat ctggcgtcgt tcatacgatg tattgcctcc aaatatggac cgtgatgatg
     1381 agcactcagc tcacacagac cgtcgttatg cttcacttga cgactcagta atcccagatg
     1441 ctgaaaactt gaaagtgact ttggaacgtg ctcttccatt ctgggaagat aaaatcgctc
     1501 cagctcttaa agatggtaaa aacgtattcg taggagctca cggtaactca atccgtgccc
     1561 ttgtaaaaca catcaaaggt ttgtcagatg atgagatcat ggacgtggaa atccctaact
     1621 tcccaccatt ggtattcgaa ttcgatgaaa aattgaacgt tgtttctgaa tactaccttg
     1681 gaaaataaaa aattgtaagc ctaggattga tttctaggct ttttatgtta gtatggaagt
     1741 atgataagga ataaaaaaca agattatgta ctggcctaca agcaaccagc ttcaaccact
     1801 tacatgggtt gggaagaaga agctttaccg ataggtaatg gttctttagg agcaaaagta
     1861 tttggcctta tagggtctga acggattcaa tttaatgaaa aaagtctctg gtctggaggt
     1921 ccacttcctg atagttcaga ttatcagggt ggaaatcttc aggatcagta tgttttttta
     1981 gctgagattc ggcaggcttt ggagaagaga gattacaatc tggctaagga actggctgag
     2041 cagcacctaa ttgggccaaa aacgagtcaa tatgggacct atctgtcttt tggggatatt
     2101 cacattgagt tcagccagca aggtacgact ttgtctcagg tgacggacta tcagagacag
     2161 ttgaatatta gtaaggcact tgcgacgact tcttatgtct ataagggaac gcgatttgaa
     2221 cgtgaagctt ttgcgagttt tccagatgat ctcttggttc aatgttttac taaggaaggg
     2281 ttggaaactc tagattttac tatagaacta tccttgacct gtgatttggc ttctgatgga
     2341 aagtatgagc aggaaaaatc tgattacaag gagtgtaagt tggatattac tgattctcat
     2401 atcttgatga agggaagagt taaggataat gatctgcggt ttgctagtta tctagcttgg
     2461 gaaacggatg gagatattag agtttggtca gatagggttc agatatcagg agccagttat
     2521 gccaatctct tcttggccgc taagacggat tttgcccaaa atcctgctag caattatcgc
     2581 aagaaactag atttagagca acaggtgata gacttggtgg acacagctaa agaaaagggc
     2641 tatacccaat tgaaatcaag gcatatcgag gactaccaag ccttattcca gcgtgttcaa
     2701 ttggatttgg aagctgatgt tgacgcatcc actacagatg atttgttaaa aaattataag
     2761 ccacaagaag ggcaggcttt ggaggagctg ttcttccagt atggacggta tttattgatt
     2821 agttcgtcca gagactgccc agatgctcta ccagctaacc tacagggagt ctggaattcg
     2881 gactatcact taaatgtcaa tctgcagatg aattattggc cagcctatgt taccaatctc
     2941 ctagaggcgg tctttccggt catcaactat gtagatgatt tgcgtgtcta tggtcgtcta
     3001 gcggctgtaa agtatgcagg aatcgtctct caaaaaggtg aggaaaatgg ttggttggtt
     3061 catactcaag cgactccctt tggttggacg gcacctggtt gggattacta ttggggttgg
     3121 tcaccagctg ccaatgcgtg gatgatgcaa accgtttatg aagcctattc attttatagg
     3181 gaccaagact atctcaggga gaaaatttat cccatgttga gggaaacggt tcgtttttgg
     3241 aatgcctttt tacataagga tcagcaggcg cagcgttggg tgtcttctcc gtcttattct
     3301 ccagaacatg ggccgatttc gattggcaat acctatgacc aatctctgat ttggcagtta
     3361 tttcatgatt ttattcaggc tgctcaggaa ttgggactgg atgaggactt gttgactgag
     3421 gttaaggaga agtctgattt actaaatcct ttgcaaatca ctcaatctgg tcgaatcagg
     3481 gagtggtatg aggaggaaga gcagtatttt caaaatgaga aagtggaggc ccagcatcgg
     3541 cacgcttccc atctagtggg actctatcct ggcaatctct ttagctacaa gggacaagag
     3601 tatattgaag cggcgcgtgc tagcctcaat gatcgtggag atggcggcac aggctggtcc
     3661 gaggctaata agatcaatct ctgggcgcgt ttgggagatg gcaatcgagc ccataaatta
     3721 ttggcagagc agttaaagac atccaccttg caaaatcttt ggtgtagcca tcctcctttt
     3781 cagatagatg gtaattttgg tgctactagt ggcatggcag aaatgttact ccagtctcat
     3841 gcagcttatc tggtacctct agctgcccta cctgatgctt ggtcaacagg ttctgtttca
     3901 ggcttaatgg cacgtggaca ttttgaagtg agcatgagct gggaagataa aaaactctta
     3961 cagttgacca ttttatcaag gagtggagga gatttgcgag tttcttatcc agatattgag
     4021 aagagtgtga ttaaaatgaa tcaagaaaaa ataaaagcga aatgcatggg gaaagattgt
     4081 atttcggtgg caacagcaga aggtgatctt gttcaatttt atttttaaga agatgttata
     4141 aggcagtaat ttgaaactgc cttttaataa ggatttaaga atataagcag ttttcaacta
     4201 gttgaaaaaa cgttataatg ataataggaa gtaatactca atgaaaatca aagagcacaa
     4261 actaggaagc tagccgcagg ttgctcaaaa cagtgttttg aggttgcaga tggaagctga
     4321 cgtggtttga agagagattt tcgaggagta taatttgttt gatagagggt gggtctgatg
     4381 gcttatattg agatgaaaca ctgttacaag cgttatcagg ttggggacac ggagattgtg
     4441 gccaatcgtg atgtgaattt tgagattgaa aagggggagc tggttattat ccttggtgct
     4501 tcaggtgcag gcaagtcaac agttcttaac cttcttgggg gaatggatac caatgatgaa
     4561 ggggaaatct ggattgatgg tgttaatatt gcggattata gttcccacca gcgcaccaat
     4621 taccgtagaa atgatgtggg gtttgttttt cagttttata atctagtttc taatctgaca
     4681 gctaaggaaa atgtggaact ggcttctgaa attgtgacag atgccttgaa ttctgatcag
     4741 gtcttgacag atgtaggtct ggctcatcgt ctcaataact ttccagccca gctttctgga
     4801 ggggagcaac agcgagtctc cattgcacgc gcggtagcca aaaatcctaa aattcttctt
     4861 tgtgatgaac ctactggagc cttggattat cagacgggca agcaggtttt gaaaattctc
     4921 caagacatgt ctcgtcaaaa gggagcgacg gtgatcatcg tgactcataa tggagctttg
     4981 gcgcccattg ctgatcgcgt gattcatatg cacgatgcca gtgtcaagga tgtggtgctc
     5041 aaccagcatc ctcaggatat tgacagtttg gagtactagc atgatcaagc gaaaaactta
     5101 ttggaaggac ttaattcagt ccttcacagg ctccaagggg cgttttttat ccatcttgat
     5161 cctgatgatg ttgggatctc tagccttagt aggcctcaaa gtaaccagtc ccaacatgga
     5221 ggcgacagct aatgcttatt taacaactgc tcaaaccttg gatttggcag tcatgtctaa
     5281 ctatggcttg gatcaagcag accaagaaga actaaaacag acggagggcg cagaggtcga
     5341 gtttggctat ttgacagatg tgactatgga taatgggcag gatgccattc gactgtactc
     5401 caaaccagag cgaatttcaa cctttcagct aagaaaggga cgacttcctc agtcagacaa
     5461 ggaaatcgct ttggccactc atttgcaagg ccaatacagc gtgggacagg agattagttt
     5521 taaagaaaaa gaagagggtc attcctcttt aaaagaccat acttatacca ttactggttt
     5581 tgtggattcg gctgaaatcc tctcccagcg agatatgggc tacgcaggaa gtggaagtgg
     5641 gactctgaca gcctatgggg tgattttacc tagtcagttt gatcagaaag tctacaatat
     5701 agctcgtttg aaatatcaag atttagcagg tttaaatgcc ttttcatcag cttatgaaga
     5761 aaaatccaag caacatcagg aagagcttga acaaatttta tcagataatg gcaaggtacg
     5821 tctgcaactt ttgaaaaaag aaggacaaga gtctctagac aaggggcaag agacccttga
     5881 caaggctcag actaatttgc aggaaggcaa gcgtcgttta gcagctgctc aagctcgtat
     5941 acaggctcaa gaaagtcaac tagccttgtt tcctcaagtt cagagagagc aggctagtgc
     6001 tcaacttacc caagccaagc aggaattggg caaggaagag gccaaactaa agcaagctga
     6061 acaaaatcta gcccaagaaa aggaaaaatt agaaaaacat cagcaagtct tggacgattt
     6121 ggcggagcca aggtatcagg tctataatcg tcagaccatg ccaggtggtc agggctatct
     6181 tatgtatagc aatgcttcat ccagtattcg agcagtgggc aatatctttc ctgtggtact
     6241 ttatgccgta gcagccatgg tgacctttac gaccatgact cgctttgtag acgaagagcg
     6301 aactcatgca gggattttta aggccttggg ttaccgtagt aaggatatta tcaccaagtt
     6361 tctcctttat ggactagtag ctgggactgt cggaacggct ctaggtagta tacttggtca
     6421 ttatttgcta gccagtgtaa tttcaagtgt cattacaaaa ggcatggtgg tgggagaaac
     6481 ccagattcag ttctattgga cctatagctt actagctctt gtcttgagct tgttggcgag
     6541 tgtgttacca gcctatctgg tggcttggag ggaacttcat gccgaagcag cccagcttct
     6601 acttcctaaa cctcctgtca aaggagctaa aatcttattg gagcgtatcg gttttatctg
     6661 gcgtcgtctc agttttactc ataaggtaac agcccgcaac atctttcgtt ataagcagag
     6721 aatgttgatg acaatctttg gtgtggcagg ttctgtagct ctgctctttg caggtttggg
     6781 aatccaatct tctgtagcag gagttccgtc taaacagttt caacaaatcc aacagtatca
     6841 gatgcttgtc tctgaaaatc ctagtgcgac caatcaggac aaggtagagc tagcagaagt
     6901 gttgaaaggg caggagatac tagcctacca gaaaatctat tctaaaacgc tagacaagga
     6961 tttcaaaggc aaggctggtc ttcaaaacat tactcttatg atgatagaga aggaagattt
     7021 gactcccttt atccatcttc aacatcatca gcaggagctg acattaaaag atggcatcgt
     7081 tattacagct aaactcgccc agctggcagg tgtcaaggtt gggcagactt tagaaattga
     7141 aggtaaggaa ctaaaggtcg ctgctattac tgagaactac gttggtcact ttatttatat
     7201 gagtcaggct agctatgagc aactttacgg acagctaccc caagccaaca cttatctggt
     7261 ctcattaagg gataccagtg caactagtat cgaaagtcag gcgggcttgc ttatgaatca
     7321 atctgcggtg tccagcgttg tccaaaatgc ttcagccatt cgactcttcg actctatcgc
     7381 tagctcactc aatcagacca tgaccatctt ggtcatcgta tcggttctat tagctattgt
     7441 catcctttac aatctgacca atatcaacgt agctgagaga atccgtgaac tctccactat
     7501 caaggttctc ggttttcata ataatgaagt caccctctac atttaccgtg agacgattgt
     7561 gctgtccctt gtgggaatcg tacttggtct ggtagctggt ttctatttac accaattttt
     7621 gattcaaatg atttcgcctg cgactattct cttttatccg caggtaggct gggaagtcta
     7681 tgtaatccca gtggcagcag taagcatcat tttgaccttg cttggtttct tcgtcaatta
     7741 ttatctgaga aaggttgaca tgttagaagc cctgaaatct gtagagtaag gtagttattt
     7801 ttagctgatt gaacttctat ttactaatat tcaaaaatcc tccgtttcaa agagcaggga
     7861 actctttgtg acagaggatt ttttctatag ggctttagca gctgcaattg cggcttcgaa
     7921 gtttggctca gaattgatat tatccacgta ttcaacgtag cgaatcgtat tgtcagtatc
     7981 gaggacaaag actgcgcgtg ctaataggtg ccattcgttg atcaagaggg cataatcgtg
     8041 cccgaaagaa tggtcaaagt agtctgaaag cataatggca ttgtcaaggc cttcagcacc
     8101 gcaccaacgt ttttgagcaa aaggtaggtc cattgaaaca gtcaatacga ccgtgttgtc
     8161 cagtccagcc aattcttcat taaaacgacg tgtttgagtt gagcagatgc ctgtatcgat
     8221 agaaggaacg acactcaaga cttttttctt gccatcaaaa tcagccagag attttttaga
     8281 aagatctgtt gtagtaagag aaaaatcaag cgccttgtcg ccgacttgta gttgtttacc
     8341 tgtaaagctc acaggatttc cgagaaaagt taccatagga tactccaatc ttttttcttc
     8401 cattgtatct gaaacagtca gaattttcca atgatttgac cggaaatgtg ggcatagaaa
     8461 aaacgccagc tcatatgaga atgacgtttt tcagaggctt attttgccaa tccttcagca
     8521 atcttgtcaa ggttgtattt catcatgctg tagtagctgt cgccttcttt accttgttct
     8581 gcgatagagt cagtaaagat ttgtgcgtag attgggatgt ttgtgtcttg agaaacggtt
     8641 ttcattggac ggtcatccac acttgattct acaaagagtg atggaacttt tgtttggcga
     8701 agtttttcaa ccaaggtctt gatttgttca ggagttcctt cttcttcagt attgatttcc
     8761 cagatgtagg cacttggaac accataggct ttagagaagt atttgaatgc tccttcgctg
     8821 gttacaatga gtttcttttc agcagggatc ttattaaatt tatccttact ttctttatca
     8881 agtttgtcta acttatcagt atattctttg agattttttt catagaattc tttattgtta
     8941 gggtctttgg cgctcaattg tttggcgata tttttagcaa aaataatacc gttttcaagg
     9001 ttaagccaag cgtgtgggtc ttcttttcct ttttcatttt gaccttcaag gtagataaca
     9061 tcaacgccgt cgctgactgc gaagtagtct ttgttttcag ttttcttggc attttctacc
     9121 aattttgtaa accaagcatt gccacctgtt tcaaggttga taccgttata gaaaatcaaa
     9181 tcagcctcag aagttttctt aacgtcttca ggaagtggtt cgtattcgtg tgggtcttgc
     9241 ccaatcggaa cgatactatg aaggtcaatt ttgtcaccag caatattttt agtaatatca
     9301 gcgatgattg agtttgtagc aacaactttt agtttttgac cagaagttgt atcttttttt
     9361 ccgctagcac atgctacaag aatgattgca gaaagaaaga gaacgagtaa tgtacctaat
     9421 tttttcatta gatcctccaa tttattaggg ctttgcccct tattttaaca aatgtttatt
     9481 tttcagtttc aaatatcgtt gtttgggagc gataaagaag ctaatgagaa agaaactagc
     9541 agctgtaagc acgatactag aacctgccgc aacattaaaa ctatagccaa taaagagtcc
     9601 caaaactgaa gcagtagctc cgaaggttga ggaaaggaaa atcatgcttt tcagactatt
     9661 agcatacaga taagcagttg cagctggggt aatcagcatg gctacaatca ggatagttcc
     9721 gacactttgc atggctgtca cagacacgag agtcaggagt accatgagaa ggtagtgata
     9781 gaaattgaca ggcattccca tggctttagc caagagttca tcaaaggaag ttatcaagag
     9841 ttgcttgaag aaaatccaga ttaacaagag gatggctgcc cccacaccca tagtaataaa
     9901 catatccgta tcttggacgg ccaggatatt accaaaaagg atatggaaaa ggtcagttga
     9961 acttttagcg acaccaatca agatgatacc gagggctaag aaagaagaaa aggtaatgcc
    10021 gatggcggta tcgcttttga taatcgagtt tcctttgatg taggtaatga tgatggcagc
    10081 tagcaatcca aagacaatgg ctccgataaa gaagtcaagg cccaagatga aggagagggc
    10141 tacacctggt aagacagcat gtgaaatggc atctcccatg agtgacatcc cacgtagaat
    10201 aatgaaacat cccacggctc cagctacaat cccgacgaca atagctgtta tcaaggcatt
    10261 ttgtaggaaa tggaattttt gcaatccatc gataaattct gcaatcatag gtcacctcca
    10321 ttgaaaaaga gttgattacc gtaagcttct tttagattgg tttcggtaaa agtttctttt
    10381 gttggaccaa aggcaatcac ttctcgattg acaagtaaga cttgatcgaa gtagtgggga
    10441 atcttgctga ggtcgtggtg aacgatgaga accgtcttcc cagctttttt caaatctctc
    10501 agcgtattca tgatgatttc ctcactgaca gagtcaatcc cagcaaaggg ttcatccaag
    10561 aggatatagt cggcttcctg caccaaacat ctggcaatca agacccgctg gaattgacct
    10621 ccagacagtt gactaatttg acgttcagcg tagtcagcta ggccgacgat ttcaagggcc
    10681 tcttgcactt tcttccaatg tttagccttt aaacttcgaa agagaggaat agagggaaat
    10741 agtcctaacg agacgcattc cttgaccttg atgggaaagt tgtagtcgat attgattttt
    10801 tgttcgacat aggcaattcg gtgtaaggat tttttaactt ccttgtcatc gagaaatgcc
    10861 tgaccttgat gtgggataat tcccagcata ccttttaata gtgttgattt cccagcgccg
    10921 tttggaccaa tgatgccggt aattgttggt ccatggagca ctagtgaaat atccttaagt
    10981 gccaacgttt ctttgtagga gacactgagg ttttcgatac gtatcataaa cttgtattcc
    11041 tcctgtctct t
//
LOCUS       DAVKLX010000062        10761 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKLX010000062 DAVKLX010000000
VERSION     DAVKLX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.062"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(120..1544)
                     /locus_tag="VW325_001859"
     CDS             complement(120..1544)
                     /locus_tag="VW325_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9735199.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2089..4368)
                     /gene="pulA"
                     /locus_tag="VW325_001860"
     CDS             complement(2089..4368)
                     /gene="pulA"
                     /locus_tag="VW325_001860"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9735200.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4480..6438)
                     /gene="ligA"
                     /locus_tag="VW325_001861"
     CDS             complement(4480..6438)
                     /gene="ligA"
                     /locus_tag="VW325_001861"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9735201.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6531..7709)
                     /locus_tag="VW325_001862"
     CDS             complement(6531..7709)
                     /locus_tag="VW325_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9735202.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMVGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLQSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7789..8652)
                     /locus_tag="VW325_001863"
     CDS             complement(7789..8652)
                     /locus_tag="VW325_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9735203.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8655..10556)
                     /locus_tag="VW325_001864"
     CDS             complement(8655..10556)
                     /locus_tag="VW325_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9735204.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKYSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYIEKRATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3235 a         2369 c         1910 g         3247 t
ORIGIN      
        1 agctgtacta ttctggtttc aatctattat aataccaaaa agttcgtatt ttttctataa
       61 ttattattat tttatagcaa aaaaataaaa ccaggaaaac aatttcctgg ttttacacgt
      121 tatttcacat caaaaacaat ggatttgaca tttgtcatcg cttcaatgct atatttaatt
      181 ccttgcactc cagctccaga acctttgaca ccaaggaatg ggaaattatc tggaccacgc
      241 tgggttttat tattaatgtg gactgtacct acttcaagtt tttcagcaat ttcaaaggct
      301 tttttgaaat catttgtaaa gactgatgat tgaaggccga attcagattc gttggcaaag
      361 gcaatagctt cctctacact agccacacga atgattggta aaacaggacc aaatggctct
      421 tcccatgcca ctttcatatc ttttgtaact tggtcaaaaa gcactggcca gagaagattg
      481 ccctcacgtt tgattggtgt aagagcctga gcttcttttt cttgtgcatc ctcaatcaag
      541 ccccaaatga agtcggctga agcattgtca ataacaggtg taatatcagc attgtcaaat
      601 ggatcaccaa ctgttaattt agaaacttct tcctgaagca aagtagctaa tttatctgct
      661 acactttcga gaacaatgac acgtttaatg gccgtgcaac gttgtcctga gtagctaaag
      721 gctcccgcaa caatttgctt ggcagcatgt tccaaatctg catcttctag tacaagagct
      781 gcatctttcc caccaagttc caacatgata ggacgcatac cagctaaacg accaatacgt
      841 tctccaatag gagttgaacc tgtaaagttg atgaagttga cttctttgtg ctcaatgata
      901 taatccccaa tttctgaacc acgacctgta atggtgttga aaacacctgc cggaatccct
      961 gcttcttcaa atgctttagc caacaagagt ccagaaatgg aaccttgtgt tggtggctta
     1021 aacatgacca cattccctgc aatcaaggca ggtgcaattt tagaagcaga taaattaact
     1081 ggataattaa agggagcaat cgctagcacg ataccaactg gttcacgacg gacaacagcc
     1141 agtttgtttt tacttgctgc ctcaaaacca ccaccttcca ttgcttgtcc agtgatacgg
     1201 agaccttcct cagcagcata acgaatcaag tctgctgtac gcactacttc tccaattgct
     1261 gctttaatcc cttttgctac ttctttggca aggatagtac caatttcttc cttatcgcgt
     1321 tctaaaatag ctgctgtttt atgtaaataa gccgcacgtt caactgctga taaagctcgc
     1381 catgctggca gggctgcacg cgcagcttgc atagcctcat cagcttcagt ctgagtcatg
     1441 gctggaactg tacccaattc ttcttgattg attggtgaat aaatcgtaat ttcttgttca
     1501 gatgatttcc attttccatt tactaaattc tgatatcttg tcaaattctt gtcctcctga
     1561 aaatgattaa gatatatttt accaaatttt cagaaaatca caaccctttt tgcaaaattt
     1621 ttgagattat tttcacaaac ttgatttttc aaagtatact caataaaaat aaaaaaaatc
     1681 cactacgtca aggcgaggct aatgtggttt gaagaaattt tcgaagagcg tgaatgagta
     1741 tcatctatag taaaataaaa aaactgaaca atttggttgg ggacagccaa accaatttct
     1801 cacaatgttt cagaaacaag ggtgtgctac tccaatttca gcctactata actgtcatag
     1861 attgctgaaa caaagtctag gtaaaagtct tcataataaa aagacctcct atcaagtgtt
     1921 caaaaacttt gataggaggt cttgttttgt gaaaatattt atcaaatttt ctatacaagt
     1981 gagctgttag ccaggttctt tctattcttt caatttcaat gaatggattt tttactaata
     2041 ctcataactg ggaatttgtc tgtgtaaaaa tagcgagata gatggtattt ataaaacact
     2101 caagacagct agactaatat catttaaaac attatcttct tttgagcgac tgttggttac
     2161 caacatagct aaatttcctg cattttcaaa ttgatagggt tctgatttag cattcacaac
     2221 caccaagagg tgttctttgc cgtgaacttc atagataagg tagccgctat gttcaatcgc
     2281 agaatgcaca aagacatgat ggtaaatttc atcatagcta gagtaagaaa aggcaccagt
     2341 ttttgtcttc aatcggatga cttgacggat aaactcaata ctgtcttgac gctcattaat
     2401 caagttccag ttcacttggt tcacactgtc aggagcatta tagctattca tcgcacgctc
     2461 tctatcatca tgggtcaact caccattttc accagtcgca accagtttgg tacgaccaaa
     2521 ttcttgaccg atttccataa aggccatccc ctgcatgagc agattcatgg ctgtggcagt
     2581 ttcgaccttg cgcatgattt gctctgaact ttggtctgga tgaagggttg ccaataaatc
     2641 gtgaagattg taattgtcat gggcttctac atagttaagc acctgatttg gatgtgtata
     2701 gcttcctaat tcacgacttc ctaggattgc tttagctaga attggctctg tcgcagcacc
     2761 actgacaaaa cctgacttga tagcaccata aacttctccc cctttgacag catcgcgctg
     2821 attgtcatta aagaaaccaa tatttggcat ctggtaggca ttgtccttct tggccttatc
     2881 ataaggggca agacctgttc ccatatccca tccttctcca tagaggataa tgttggagtc
     2941 gatttcatcc aagctttgac gaatcatctg catggtcttg acatcatgaa tccccatcaa
     3001 gtcaaaacgg aagccgtcaa tattatattc ctgcacccag tatagaagag aatcaatcat
     3061 atacttgcga aacatttcgt gttcactggc tgtttcattt ccaacacccg ttccattctg
     3121 gaaggtacca tctggattca tacgataata gtaatcaggg actgttgttt ggaatggtgc
     3181 atcaacaact gagaaggtat ggttatagac tacatccata atgactccaa tacccgcatc
     3241 gtgataagct tgaaccatca ccttcaaatc acgaatgacc tgagctggat catctggatt
     3301 agttgaaaaa ctagtttctg gcgcgttata gttttgtgga tcataacccc agttgtaggt
     3361 tacatttcca tcctcatcgt attctttatg acggtctgca attggttgca attgaacata
     3421 attgtagccc agcttcttga tgtaatcaaa agcagttgac tggccgtatt ggttaactgt
     3481 tccagcctga gcagcaccca agaaagttcc tcgaagatgt tcatctacac ccgatgtagg
     3541 tgatttagtc aaatcacgaa tgtgcatttc acagataact gccttacatg gattttccaa
     3601 gcgccaagta gcctccgaac cgtgcttaac ctcgaagttt tcaacttgct tttctacatg
     3661 gctcagaata gctgaacgtt tgccatcagg gctggtcgcg attgtataag gatcacgtgt
     3721 cagtgtttgg tgatgaggga attggacttg atactgataa gtcttaccta ccaaatcttc
     3781 ttcaacatcc aaactccaga caccgattgt attgtcctta tgattataag agtagctatt
     3841 gcctcttttc atctcaaaag tcttccaaac gggtgcatca ttagcagctg attcataaac
     3901 gacaacttgc acttctgtcg ctgtaggtga ccagagggaa aaatgagcct gattgtcctc
     3961 tacacggcaa cccaattctc cttggtaacc ccaatgatga tcaaaactag cactgttaat
     4021 ggccttatca aaggcaaaag gattttgatt tttatagaaa ggactggcaa tagcaggatt
     4081 ttcagagtaa taaatcctat catcgccttc caaaatccag acctctgtta ataggggata
     4141 gtgattaaaa cggatagaat attctttact agtttgacct gtatgaacca caaaattcaa
     4201 gctttctata acatgtgaac ttgggtgttc aaagctaaat aaagctccaa aataatcttc
     4261 tttgtaggtt agcaaatcaa ttcgttgatc ctgacttttt acaaaggagc aagtgtcata
     4321 ttctccattc ttacgatggt aatgaatgcg catagggtag ttatacattt tttatttttc
     4381 ctttttactt tgtttctatt tcactaataa atttttgtca atctcgtctc aattaacaga
     4441 catagtcata ttctctaaac tctgttttta aacgatccat tacaaacttt ctagccatgc
     4501 ctcatctctg acctggatac caagttcttg tgctttttgc agtttacttc cagcgtctgc
     4561 acctaccacg acgaggtcgg tctttttaga aacactacct gtcactttgg cacccagact
     4621 ttcgagttta cttttagctt ctgagcgctt gagtcgttcc aattttcctg tcaataccac
     4681 ggtcaaacct gacaaggccg catccgctac taccgtctgt cctttatagt ccagattgac
     4741 cccagtttct ttcaattctc tgagcagaat ttcagagcct tctgtcgcaa aataagtctg
     4801 aagacttttg gcaatcacgc cacctagact ttcaatacta gccacttcct ctgaatctgc
     4861 ctgatacaga ttttcaattg aatggaaata ttgaagtaaa agctgactag ccttgcttcc
     4921 gacatgacga attcccaaac caaataagag cttctcggca gaattttcct ttgatgcttg
     4981 gatagcctga tacagtttag cagcggactt ttccttaacc ccctctaaaa ggaggaaatc
     5041 ctcttcttgc aaacgataaa tatccgccac atccttgact aaattagcag caaaaagctt
     5101 ctcaacaata gatggaccaa ggcctgtaat attcatagca tcacgagaag caaagtgaat
     5161 caagccttcc atgatttgag cagggcaacg cggattgata caacgtaggg ccacttcatc
     5221 ttcaaagtgc aacaagtcag agttacaact tggacagttt gtagggatat ctagtttttc
     5281 ttcagaaacc cgtttggact ctaccacacg taaaacggca gggatgatgt caccagcctt
     5341 atatacaatg accgtatcgt cttttcggat atctttttca gcaatataat ctacattgtg
     5401 cagggtcgca cggctaacag tcgtaccggc aagttgtact ggtgttagat tagcagttgg
     5461 agttacaaca ccggtacggc caactgtcca gtcaactgat aagagttgag cttctttttc
     5521 ttcggcaggg aacttgtagg ctactgccca ctttggagcc ttaactgtaa aaccaagttc
     5581 ttcttgactt gctaggtcgt tgaccttgat taccactcca tcaatatcgt aaggcagatt
     5641 ttcccgttcc tgtcctactt cttggataaa attccagatt tcatctatgt tttcagccaa
     5701 gattcgctta ggattgacca caaaacctag ttgttctagg tacttcaaac ccttttcttg
     5761 gctatcacga gttgaagggc tggcttcttg atagagaaac gttgcaagat tacgcttggc
     5821 aactactgct gtatccaact gacgcagagt tcctgctgcc gcattacgag gattagcaaa
     5881 ttcaggctct ccattttctt ggcgcacttg gttaacttgg tcaaaggaag cgcgtggcat
     5941 gtaacattcc ccacgaactg tgatatctag ttcttctggc aaagtcaaag ggatgtcctt
     6001 aacacgcttg aggttttctg tgatattttc accaattgaa ccatctccac gtgttacccc
     6061 agcaaccaaa atcccctttt cataagtcag cgagatagat aagccatcga ttttcagctc
     6121 acaaatatag gtcggatgag ccacttcctt acgaacacgc gcatcaaaag catctagctc
     6181 ctcacgtgaa aaagcatcct gcaaactata aagaggatac tgatgactgt atttttcaaa
     6241 accatctaaa accttgccac caacacgatg agtcggactg tctgctagca cttgctctgg
     6301 ataagcagtt tctaactcga ccaactcacg gtaaaggcgg tcatactcac tgtctgaaac
     6361 cgagggatta tcgctggtat agtactcagt cgcatagcga ttgagcaaag cgactaactc
     6421 attcattctt ttattcataa gaccatttta ccataaaaca agccctcctc acaaacgaga
     6481 agggcggaaa aaacacttag tttgaaatta tttttgaaac tcaagcaacc ttatatcaat
     6541 ttttcaaaat gagttcgaac ataaataaac gatatacaag acaagatgat aacaccactt
     6601 ccaattatca ggaaagaaga gagatgtaca ctttgcaaga ctgtcataaa tccttttgca
     6661 ataggcataa atagaatagc taaggtaaaa attgtactca gtactctccc aagaaattcg
     6721 ctctcaacct tggtttgtac ttgagtaaaa aagtgaatat taaaaatcgt cataaacaat
     6781 tcacaaacta aatttccaga aaaggaaaga aaagttggaa gtggtaatcc catcataaaa
     6841 actccgacac ctgtcaaagc cagtaaaatc aaaagattat aaatattagc tttaatttta
     6901 ctagctagaa gagccccaat gatggaacca atagccccca tagttaaaat acttgcatag
     6961 gctccttctg acccgtaaag ctgattcgaa aagggaagta gaaattcaaa agctgcaaaa
     7021 aagaaattaa cgctggaagc taccagcaaa aggaagaaaa tttcttgctg atgccagata
     7081 tagtgtaacc catccttgat atctacaaaa atatctctcc cagtaaaagc ctttttctct
     7141 tgaacttttg cttcctcttt tggaaggaaa gccactagaa caaaagcaat gaaaaaagtc
     7201 agcgagtcta gcagtagcgt catatggaga cttgcaaact gtaaaacaag gaaggaaaga
     7261 acaggagagc taacacctac aacctgcaaa accagctcta agcgagaatt atagatcaca
     7321 atctcatctt tctccaccac ttcagttatg atagctttat tggctgtgcg agaaaaggca
     7381 aaagcaatag cctgcacaat gttagcaaca atcaaagcgc caaccatcca gctatcattc
     7441 cttatgaaag aaatagccag acaaagaatc ccacaaacaa gatctgccgt cattaaaatc
     7501 ttacgacgag aaaaacggtc tgaaataact ccgccaaagg gattgacgag aatagatgtg
     7561 acgagctcag aaatctgata cattcctaaa actgtctgtc ctatagtccc catagaagcc
     7621 aaccagacac tatttccata atcatagagc atatttccca ttttattgat agccccacgg
     7681 ctaatcaact gcactgcata gcgattcata ttaaagctcc tctcaaattt tgaaactatt
     7741 gtatcaaaac cgaaaggagc tttttatttt ttcccttatt tgagaaaatt aacttttgac
     7801 aaatttttcg tagtgttcct gataataggc tacttgctct ggaagaccta acacatcaaa
     7861 aatatgcatg gcctcttgca tctgcttaca gccttcttta cactgtcctt tttgatataa
     7921 ggcaaaacct tttaaataat ggaaaacatt acgctcataa agcttaatac ctttgtcaat
     7981 aatcttctct gtataagcct caaaatagtt ggcattataa aaagaagaat gctctaaaca
     8041 atgctggtaa caattgaggg ccaaaattaa cactaatctc ttatggcgac taatctcttg
     8101 gtaaaattcc tccctctcca taacttctct accaatccga gtgacatagt ctacatcgta
     8161 gaaactatag aggttaccga aaagaatcaa ctcatacatg gtccattctt ctgttttgaa
     8221 gagataatct gctaccttat ccaaatcatc ctgcttcata tcataagtcg catctctttg
     8281 acaaatcaga ccttgtagca aaatccagtt cagctcaaaa taaaggggag tcgtcgaact
     8341 cttagacttt tcaagttgtt ctctttgaag cttttgaaaa cctgcaatat cgtttgaata
     8401 gtaaagtggg ataatctgtg ccatcataga cacatgttca tgattatgaa aattccttgc
     8461 cttatccatg aaattttcga ttgttacatg aatgttatcc aaaatctcaa agaaacggga
     8521 gactgccagg tcagactccc caagctcaaa gcgagataac tgagaggtag agcaggattc
     8581 gcctgctgct tcctttaaag aataatttcc acttgttcga aattcacgaa atacttttcc
     8641 aagatgttcc atttttacac ctgctctgat aattcttccc actcaagcat agcttcttcc
     8701 tgacgatggc tgattttgtc cagctcagcc tgtaattcca tgagtttgtc ggcatcgttt
     8761 gtttccaaca tttgttcaga aatggcttgg ctttgacttt ctagctcttc aatttcagct
     8821 tctagacttt cgatttgtcg catgagtttg cgaacttctt tttgactttc tttctgggcc
     8881 tgatagtcat tgactggact tgcttccttt gcttgattgc tagttgaagc ttcctcagtc
     8941 tgactcattt ctgctgttgc tctcttctca atatagtagt cgtaatctcc aaggtagaga
     9001 gttgaaccat tctcagacaa ttccaaaaca tgagttgcca cacgattgat aaagtaacga
     9061 tcatgactga caaaaagcaa ggtaccatca aagtcaatca aggcattttc tagcacttcc
     9121 ttactatcaa tatccaaatg gttggtcggc tcatccagaa tcaaaaagtt attgttttcc
     9181 atagacaatt tagctaaaag caaacgagct ttttcgccac cagatagcat gccgactgat
     9241 tttttaacat catctcctga gaaaaggaag gctccaagac ggttgcggat ttcaacttct
     9301 ggtgtcagtt tgaaatcatt ccagagttca tccagcaccg tattacttgg tgtcagcttg
     9361 ctttgggttt ggtcatagta accaacctca acattagcgc caaagcgctt ttcacctttg
     9421 ataaaaggaa tctggtccac aatagacttg ataaaggttg acttgccgat accatttgga
     9481 ccaacgatag cgacagcatt catcttacga agatctaggt tgataggttg tgacaaaact
     9541 tccccgtcat aaccaatagc agtattttca acagtcaaaa caacattgcc cgacgttttt
     9601 tcagactgga aggtcatgtt ggctgctttc ttgccagctt caggcttgtc caaacgctcc
     9661 attttttcta gttgtttacg gcgagattga gcacgtttgg tcgttgaagc acgaactaga
     9721 ttgcgattga caaagtcttc cagagcagcg atttccttct gttgcttttc atagtttttt
     9781 gcctcagtag ctagcttttg ctccttcaac tcaacaaaac gagagtaatt ccccacatag
     9841 cgatccaagg aatacttggt caaatctagc gtaattgtcg caaccttgtc caagaaataa
     9901 cggtcgtggc tgacgataat gagggcaccg ctatagttta ccaagtaatt ctctagccag
     9961 gagatggttt caatatccaa gtggttagtt ggctcgtcca agaccaagag attgggcttt
    10021 tcaaggagca ttttagctag agccaaacga gtattttgac caccagaaag ctcagcaatt
    10081 ttcatctgcc acatagactc gtcaaacttg aatccattca aaatcgctcg aatatcagct
    10141 tcataggtaa agccacctgc ttggcgaaaa ttttcagata agcggtcata atctgacatc
    10201 agtttatcca aatcctcacc agacttttca cccatctcca gctccatctg acgcagttgt
    10261 ttatctgtcc gacgtaagtc atcaaagaca tgaagcatct catcatagat ggtattttca
    10321 gactcaaaac ggctatcttg agctaggtaa gacagagaaa tatctttttt cttattgatt
    10381 tctccactag ttggctcctc ttctccaact aaaatcttca aaagagtaga cttacctgca
    10441 ccatttttcc caacaagggc aatccgatct cgttcatcaa cctgcaggtt gatattatcg
    10501 aaaagaacct ctcctgcaaa agaacgttca attttattag cttgtaaaat aatcatacaa
    10561 gtagtatagc atgtttccct aaggcattca agataatcgt aagtctttta gtacaacttt
    10621 tataacataa aataaactaa attatgtata ttttatatta gattccttca ctatcttgtt
    10681 ggattttcta accagctaat cttgtttcaa atagttatcg cacaagccta ttatttaatt
    10741 cttttcatta tttacgtacg c
//
LOCUS       DAVKLX010000063        10664 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKLX010000063 DAVKLX010000000
VERSION     DAVKLX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.063"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(8..1222)
                     /locus_tag="VW325_001865"
     CDS             complement(8..1222)
                     /locus_tag="VW325_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9735205.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1378..1672)
                     /locus_tag="VW325_001866"
                     /pseudo
     CDS             complement(1378..1672)
                     /locus_tag="VW325_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1834..2286
                     /locus_tag="VW325_001867"
     CDS             1834..2286
                     /locus_tag="VW325_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9735206.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2321..3709)
                     /locus_tag="VW325_001868"
     CDS             complement(2321..3709)
                     /locus_tag="VW325_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9735207.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3775..4737
                     /locus_tag="VW325_001869"
     CDS             3775..4737
                     /locus_tag="VW325_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9735208.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(4943..5953)
                     /gene="ccpA"
                     /locus_tag="VW325_001870"
     CDS             complement(4943..5953)
                     /gene="ccpA"
                     /locus_tag="VW325_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9735209.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6378..6977)
                     /locus_tag="VW325_001871"
     CDS             complement(6378..6977)
                     /locus_tag="VW325_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9735210.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEMVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(6979..8076)
                     /locus_tag="VW325_001872"
     CDS             complement(6979..8076)
                     /locus_tag="VW325_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9735211.1"
                     /translation="MLERLKSIHYMFWISLIFMVFPILTVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGILLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     AKQNAQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(8076..8813)
                     /locus_tag="VW325_001873"
     CDS             complement(8076..8813)
                     /locus_tag="VW325_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9735212.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAIFSFPVVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVDMSLGNWIGAALLLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(8815..9699)
                     /locus_tag="VW325_001874"
     CDS             complement(8815..9699)
                     /locus_tag="VW325_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9735213.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENIIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(9686..9880)
                     /locus_tag="VW325_001875"
     CDS             complement(9686..9880)
                     /locus_tag="VW325_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735214.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(9877..10083)
                     /locus_tag="VW325_001876"
     CDS             complement(9877..10083)
                     /locus_tag="VW325_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735215.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         3286 a         2416 c         1899 g         3063 t
ORIGIN      
        1 gcaagcccta tctacgatac tgtttcaaga aacgagtcat cttttcttgg atttgggcta
       61 actcatcaac acgaggaagg taaacgatac ggaagtggtc tggttcctgc cagttaaagc
      121 ctcgaccatg aaccaagaga accttttcct gcttcaagaa atcaaggaca aactgctcat
      181 catcatcgat acggtacata ttgcggtcga tttttgggaa gatatagagc cccgccttgg
      241 gtttaacggc agacaaacct ggaatatctt gaatggcatt atagatgaaa tttctttgct
      301 cgtagattcg tccaccagga agaagcaatt catcgactga ttggtgaccc cccaaggaag
      361 tttgtacgac ttgttgggcc aaaacgttag agcaaaggcg catattggac agcatattga
      421 gcccttcgat atagccctta acatgagtct tagggccaga caagaccatc caacccacac
      481 ggaaacctgc tatgcggtgg gattttgaca gaccattcat gctgacacag aagacatctg
      541 gtgccaagct cgccacaggc gtatgcacat gtccgtccat taccatgcgg tcatagattt
      601 catccgcaaa gataatcaaa tcgttttgac gggcaatctc aataatctcc agcaagagtt
      661 ccttaggata aagggctcca gttgggttat ttggattgat aaggacaatt gccttggtat
      721 tggaagtgat ttttgacttg atatcgtcaa tatctgggta ccattccaca gcttcatcac
      781 agatatagtg aacggcattt cctccagcta ggctgacagc cgccgtccat agaggatagt
      841 ctggcattgg cactaagacc tcatccccat tgtccaaaag cccctgcatg gacatgacaa
      901 tcaactcact gacaccattt ccaaggtaaa tatcatcaat atctacgttt gggaatttct
      961 tcagctggca atactgcatg atggccttac gggctgagaa aatccctttg gagtcagaat
     1021 agccttcact atcacgcgca ttcataatca agtcatgaat gacctcgtct ggcgctgtaa
     1081 agccaaattc tgctggattt cctgtattca gacgtaaaat cttttctcca ttcgctcgca
     1141 tccgcatggc ttcttccaat acaggaccac ggatatcata agcaacatgc tctaacttac
     1201 tagacttgtt atattctttc atcttgtttc ctcaattcat caggatagta acattatacc
     1261 actagaaaga gaatgcgaca agtttactga aatgtgttac tagtttcgta tagaagaaaa
     1321 accttgctaa aggcgaggtt aagaacgtag agatagcagg tattttgcaa agacacatca
     1381 cataccaaaa gataaaccta gaaaaaagca agcctagaaa ggagtcataa agaagattaa
     1441 acatgaggaa aagcccccat atcatcaagt aatcccaacg cccacttcgt tttgcaacta
     1501 gaaataagat tagatggtag agtacatgaa aaactaaaaa tccaataaaa cttggataaa
     1561 gaagcggtac cagactctct aattgacaga tcatgatcac ttctaccaag actgaaacta
     1621 ggataattcc ataataaagg aaatttgatt tttgaaaaga gtttttcatc attctccctt
     1681 ccttcacttc tttcctgcta tcctttcttt tattttatca tatatttata tagagtttaa
     1741 gccctaatat tcttttgaaa aatacttttt ttgctttact tctatagtga aaaggtttac
     1801 aattatagta agaaaatttc aggaggtttc attatggcac aacgttacca aaatatcatg
     1861 gtcgcaatcg atggttctaa ggaagcggac ttggcttttg tcaagggagt tcattctgct
     1921 ctacgaaacg acgctaaact caccatcgca catgtcattg acacacgcgc tctccaaagc
     1981 gtatccacct ttgatgctga agtttacgaa gaactccaag tcgacgctga aagtctgatg
     2041 aaagagtacg aaaaacgtgc taaagatgct ggggtagcag atgttcatat cgtcattgaa
     2101 atgggaaatc caaagaccct gctagcacgt actattccag atgccgagga agtggacctc
     2161 atcctcgttg gcgcaactgg tctcaacgcc tttgaacgcc tcttggtcgg ctcttcatct
     2221 gaatacatac tccgccatgc taaggtcgat ttgctggttg tgagagaaca agaaaaaacc
     2281 ttataatcac aaagaaaagg agcccctagc tcctttttgt ttacgattta tttctctctt
     2341 tatggcgttc gtaagccttg agctggcgct gcagttcctt tttaatagca ggttctggag
     2401 catatttttc ttcccaatta tctggtttta agattttatg ggtcactgga tcaaaatgag
     2461 ccttgccatc tggaaaaatt ttccccatat tggcctgatg gacaatatca aaaatacgtt
     2521 ctgggtccac ccccatcaag acaaaactgc cgtaggtgaa gtaaagcgtg tcaatcaagg
     2581 catccacttg ccctatcaaa tcttgctgag caggtgtctt cttggctact ttacctgctg
     2641 ccttatcaag ggcctgatga agttgcgata cagcttgacc aaaatcttct tcagaaggac
     2701 tggctgatcg aacaaactcc accaattctt ctattttaaa accagcccta tgggttgcac
     2761 cctctaaatc ccaagctcga ggttcttctt gggttcgttc atccatcatg tggtggaagg
     2821 tcttgacctt attgaaatga tagtcacggc tgacaaaaac tttttctgaa gacaaaacac
     2881 caaaatgttc caaggccttg tggatcccat cttgttgatt gctagtggta atgtgcttgg
     2941 cagcttcctt gacactgcta ctaccatttc ccatagcgac cgacataccg acgccagcca
     3001 gcatttcaag gtcattatct gagtcaccaa aagccatgac ttggttgagg tcaaagccat
     3061 attctttccc aactcggcga atgccttcta atttagaatt tccctgattg atgacatccg
     3121 atgcaaaagg attgctacgt gtcaatttca agtcttcaaa atcagctgcc gccttctcag
     3181 attcttctgg tgtcatcagc atcaaaactt ggtaaatagg ttgattcatc aggtgaagca
     3241 ggtcctcttc cttttgggga acaaccttgc tgaccatgcg attaaaagac tgactcaccg
     3301 tccgagttaa aacagaggga acgaagcgac taattcgttg ggaaaaagaa cccagaccaa
     3361 aggacatgat tttagaaccc aacatggcat ccttggtccc taaggcaatc tccgtgccct
     3421 cttttttagc atagctaatt agatggcgca aatgtaactt ggaaataggg ctcgtgaaca
     3481 agactctgtc tttactaaag atatactggc cattataggt taccgcaaaa tccagatcca
     3541 aatcgtccat caattcctta acaaaaaaag gtcctcgccc tgtcgctacg ccaactagta
     3601 ccccttgttc tttgacaatc ttaatcgcat ccttagtgga tttcaaaaca ctcttgcgat
     3661 tgttgaccaa ggttccatcg atatcaaaaa aaacagcttt gacttccatc ctatcccaat
     3721 ctcccctttt gtgatacaat gattatacca catttcagaa agagtgagta aatcatgcct
     3781 aagaaaatcc ttgttttaca tacgggtgga actatttcca tgcaggccga tgcttctggc
     3841 gctgttgtga cgagttcaga taatcccatg aaccatgtgt ccaacccact tgaaggaatc
     3901 caagtccacg ccttggactt ttttaacctt ccaagtcccc atatcaaacc caaacatatg
     3961 ctggtcctct accagaaaat taaagaggaa gcagataact acgatggagt ggtgatcaca
     4021 cacggaaccg atactttaga ggaaacagcc tatttccttg ataccatgga agttccccat
     4081 atgcctatcg ttctaacagg agccatgcgt agctccaatg aactcggtag tgatggtgtt
     4141 tataattacc taagtgcttt acgagtggcc agcgatgaca gggctgctga caaaggagtt
     4201 ttggtcgtta tgaacgatga aatccacgct gccaagtatg tcaccaaaac acatacgact
     4261 aatgtcagca ccttccagac tccaacacat ggcccccttg gtctcatcat gaaacaggaa
     4321 atcctctact tcaaaacagc tgaacctcgt gttcgctttg accttgatca catacaaggt
     4381 ttagtcccta tcatctcggc ttatgctggt atgacagatg agctgattga tatgctggat
     4441 ttagaacact tggacggttt gattatccaa gccttcggag ctggtaatat tcccaaagaa
     4501 acggctcaaa aattagaaag ccttctgcaa aaaggaattc cagtcgctct ggtatcacga
     4561 tgctttaacg gtattgccga gcctgtttat gcctaccagg gtgggggcgt acagttgcaa
     4621 aaagcaggcg ttttctttgt taaagaactc aacgcccaaa aagctcgctt gaaactcctc
     4681 atcgccctca atgccggact aacaggacag gctttgaaag actatatgga aggctaatac
     4741 tcttcgaaaa tctctgcaaa ccacgtcacg tcgccttacc gtatgtatgg ttactgactt
     4801 cgtcagtttc atctacaacc tcaaaacagt gtgttgcgca gcctttaacc agccgcctag
     4861 tccgctctat ggtattcatt aagtcaacat ctcttgttta agagcacaaa atcaggaaat
     4921 cttctcgatt ccctgatttt ttctatttac gttttcgtgt tgagctacgt tctgtcaaac
     4981 catgaggtaa gagaacttca cgttcttcca actcttcctt atgcataatc ttggtcaaca
     5041 tacgcatact aatggcacca aggtcataaa gaggttgggc aatcgttgtc aagtttggac
     5101 gggtaaagcg tgagatttgt gaatcatcac tagtaataat ttcaaaatct tctggcacag
     5161 aaacaccctt atcagccaaa ccgttcaaga ctcctgctgc caactcatca cctgtcacaa
     5221 ctgctgcagt tgcatttgat gaaatcaaac gctctgctaa ggcgtaacca tcatcatagc
     5281 tatatttaga ttcaaatacc aaaccctcac tataagtgat tcctgctttt ttcaaggttt
     5341 ccttgtagcc aactaaacga accttaccat tgatgtcatc cactagcgga ccgctaacga
     5401 aagcaatacg ctcattttct ttagcaaggt aactcactgc atcaattgtt gcttgcttat
     5461 agtcaatatt gacacttggc aactggtgct caacatcgac agttcctgcg agaacaatcg
     5521 gagtacgtga acgcgaaaat tctgagcgaa ttttatctgt caagtgatac cccatataga
     5581 taatgccatc tacctgcttt gaaaagaggg tattgacaac agaaacttct ttctcgttat
     5641 cttcatcgct attagctagg acaatattgt acttgtacat ttctgcaata tcatcaatcc
     5701 ccttagccaa actcgaaaaa taaccattgg taatatttgg aatcacgaca ccgacagtgg
     5761 ttgtcttttt acttgcaaga ccacgcgcaa ctgcatttgg acgataatcc aaacgatcaa
     5821 ttacctctag cactttttta cgggtattct cttttacatt tttattgcca ttgaccacac
     5881 ggctgaccgt cgccatggaa acacctgctt cacgagcgac atcataaatg gttactgtat
     5941 catctgcatt cattcctttt cctgtccttt ctatctcaca cattctttta caagtagagg
     6001 tactgattga agctctatat ctacttacaa aagtgaagat gtgaaaattt cgttttcata
     6061 tttctactta ttccattcta tcactaattg taaacacttt caagtgtttt ttgaagattg
     6121 attgaaaaaa tttcatagaa aacctaggtt tagctccttg ctaccacctt agactaaaca
     6181 aaaaggagga aactaagccc tcctaaagtt atagtaaaat gaaataagaa caggataaat
     6241 cgatcaggac agtcaaatcg atttctaaca atgttttaga agtagaggtg tactattcta
     6301 gtttcaatct actataggta ttgttccatt cactaccgtc aattttagca catagtcttc
     6361 atgaaaatat tatatcatca taaccaacca gattcttttg cgatattagc tgcctctgtt
     6421 cgattaccag catccagttt tgaaagaata ttggtgacat agtttcgaat agttccgttt
     6481 gagaggtaaa gctgatctgc gatttcttgg ttggataagc cccgggcgat tccctttaag
     6541 acagcaatct cttgttctgt caacggattg ggacgggtca tcaccatttc catcaattca
     6601 ggcgaatact ccttgcgtcc ttcgaggacg gtgtgcaagg tttgcatgag gtctgcaatg
     6661 cttcgttcct ttaatacata agcatccact ccagccttga ccgcacgttc aaaataccca
     6721 gcacgcttga aggtcgtcac cacaaccacc tttgtttcaa gcttttctga tcgtatccac
     6781 tccaagactt caagacctgt cttaacaggc atttctacgt caaggatggc gatatctaca
     6841 gactcctttt ctaatagttg gattgcttct tgcccattct tggcttgaag gacagactcc
     6901 acatctggtt gaagcgtgag caattgacac atggcatctc gcaacatact ttgatcttct
     6961 gcgactaata ctttcatctt agtttctctc cttataaggt agtcgaactt gcacttccgt
     7021 tggatgtttc tgactgatta cacttacttc tcctgaaaat ggaaaaacac gatctcggac
     7081 tgtatggagc tcatctcctt ttagaaaagc aaagccgcag ccatcatcac ttactgttaa
     7141 aatgagttcc ttctctgtcc gttctaattt taagtaagct ttagacgctt tggcatgttt
     7201 gatgatattg gtcaccaact caagcaaaat catagaagcc attgactcca attcttgagt
     7261 aaggctagca gtatctagtt ggttatccgt ttccacctca attccagcaa tttctaacat
     7321 ctttttcaca gtctctagtt cggatgtcaa agttctagac ttaagatttt ccacgatagt
     7381 tcgaacttca ttcatggaat ccttgctgat ctggtgaatt tcttttaatt ccttttccac
     7441 ctgtggataa gcctccatct gaaataactg caaggctaga tcagttttaa cactaagcat
     7501 agcaaaggta tgccccagac tatcatgcaa atcttgaccg atacgattgc gttcattttc
     7561 agcaagcaat agatttatct gggcattttg cttagcctgt gcttctttca aatcctccac
     7621 aattcgaatc cgaaccaagc caaaagtcat taaatcgaca aaagcaagaa ttacaagtag
     7681 atagaataga aactcaactt cgattctctg aaaaatcaac agttgaccca caacaaggac
     7741 ttgagcaaga agaaaagtcc agacatgtaa agactttaaa ccacctacgc tgaaatgata
     7801 gcttaagaga ttggatagga aaaagaaaaa ccagatataa ttaacagcca caaaggcagt
     7861 attcccaact acataagtca gcaggatgcc ccaatatagc caagatagac gctggctctt
     7921 agttgttaaa acacccaaat acgccactac aaatagaata tcaatcaata aatgccaggc
     7981 agaaagccac ccagtcacta cagttaggat ggggaaaacc ataaaaatta aactgatcca
     8041 aaacatataa tgtatgcttt tcagtctttc aagcattaag cattctcctt atgaccttga
     8101 aggtaaatgg tcaaaccaaa caaaactact gaaaaaacaa gtaaataaac tgtggctgat
     8161 agattgatgc caccctcatt taagaaggtc ttgagcaact ccatcaactg ataggtcggg
     8221 agacacttac ctactacttg catccagtct ggaaataaag agataggcat ccagagtcca
     8281 cctaaaacag ccaaccctag ataaagaaga ttgcccacga cagacatcaa ctgactagtt
     8341 ggtaagagag tcaaggtcaa accaagcgct acgaaggcaa tacttcctac tatcagcaaa
     8401 agtgcagccc caatccaatt tccaagagac atgtccacac ctcttacaaa atgcccaact
     8461 gagaaaacca ccaagattga aaccaaataa tcaaccagca tacttgttat ctttgataga
     8521 taatattcta ccatatttac agggctatga cgcaatgttt tctgccagtt gttgatcttg
     8581 tcggtatgta aaacaactgg gaatgagaag atagctgttg acatcatgga aaatgcagtc
     8641 atggagataa gataatcacg cataaaattc gcgagttcac ctggtgtgtc ctgatagata
     8701 ccagaaaaaa ataaatagaa agccgtcggc atccctaccg acaatagata atagattaat
     8761 tgtcgtttgg tcaataaaaa ttctatctta ctaagtgcta tccatcgttt catcttagtt
     8821 atctcccttc tgcgtctctt caaagattgt attcaacaaa ctacgattat taacttcgat
     8881 ttcttgcatc ctacatcctg cttgggctaa tagttcccag aaagcatctg cttctcgagt
     8941 gactacttgt aaagaatctt gctttagggt ccagttttca accaaatttg actgctctac
     9001 gacttccttg taagctatag gaaggataaa gtgcttttct atttcctcgc tacgcatagc
     9061 tagaggcgtc gtatctcgaa tcaactctcc cttatttaag agcaaaatgc ggtctgcagt
     9121 gtgttccact tcctcaatat aatgggacga atagagaatg gtgactcctt gcgcttttaa
     9181 ctcctggaca atttcccaaa aacgttgacg agttgaggta tccatggacg cagtaggctc
     9241 atctaaaaaa acaatctttg gtcgcccaat taaggtcaag atgaaagaaa agagacgctt
     9301 ttgaccgcct gataattttt ctgccaactg ttctttttgt tgcttgacaa actgcaatag
     9361 ttgatcaatt tcctgattgc tcaaaggatt tggatagata cgttgaaaga aagcaatcaa
     9421 ctctttaact tttaatttct gaacgattat attttcttga gggaggtaag ccctagtata
     9481 gtctaactta gaactcgtca ctggtaaacc ttgaatagat acttgaccgc ttgtgaccag
     9541 tttatctcca agcaaacaat ctaagagtgt tgtctttccg gcaccatttg gaccaattaa
     9601 ggcaacacat tcaccatcgt tgatttcaaa ggagatgttc cgcaaaattt ccttgtcttt
     9661 tattttctta ctcaattttt caactttaac cacagtcatg agactctcct ttcaaccact
     9721 ccattcccat agggaaaacg acgaaaatca taaatccaaa ccccaaagca ccacaaatga
     9781 attggcgaag caaggcttga tcaaaccaat cagtaaacat ttccactaac cataccaaga
     9841 gtgacaggcc gataaagaaa tagatgatcc ctctcttcat tcctcaaact cctttttcac
     9901 atctctgact aatttcaaac cttctctaac aagccaagac atgattccaa agccagcaaa
     9961 gaccttccaa ggaaaatggt agaaatcctc atccaatccc gaaaacatga gataggtcat
    10021 aactcctgct actactaaac tcattgcaat aatcatttta tttctcatct cttcttcccc
    10081 catttcatac tacaattata gtcttttaaa atcagaggag gcagaagctt ctgtcactag
    10141 aaaatatgac aaatgtcata aaaattctgt tcaaaacaag caagatacac tatacaataa
    10201 aacacaatca gaaaaatcta aggcaacttc ctcaaaagag atatcaaacc caattcacac
    10261 cataatgtaa actaatactt atttaaaatc aaaaagagta gaaattttta tcagacaaac
    10321 acatatatag tgtattgaat ctataacagt aggccttaaa tactaaaata tttctataaa
    10381 ttaatttaac tttcctgata gagctgttca tatcttattt caattctcta tattatacgt
    10441 tgaacaaaac ccttctattg ctttcttaaa aatttataag agttataaaa tctgttaaat
    10501 ttcaatgtgt atacctaaac tacggtattt attgaaaaga ctggagacaa aaagtatacg
    10561 ctgccaaaat gaattactga aaatcaaaaa agagagaacc aaactgattc cctcttaatg
    10621 tatataatat ctagttttaa aaatacacac tcacatatct ctgt
//
LOCUS       DAVKLX010000064        10583 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKLX010000064 DAVKLX010000000
VERSION     DAVKLX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10583
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.064"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            219..887
                     /locus_tag="VW325_001877"
     CDS             219..887
                     /locus_tag="VW325_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9735216.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LSQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGDSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            847..1524
                     /locus_tag="VW325_001878"
     CDS             847..1524
                     /locus_tag="VW325_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9735217.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            1525..2283
                     /locus_tag="VW325_001879"
     CDS             1525..2283
                     /locus_tag="VW325_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9735218.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKNFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            2295..3156
                     /locus_tag="VW325_001880"
                     /pseudo
     CDS             2295..3156
                     /locus_tag="VW325_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(3236..3883)
                     /locus_tag="VW325_001881"
     CDS             complement(3236..3883)
                     /locus_tag="VW325_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9735219.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(3880..4578)
                     /locus_tag="VW325_001882"
     CDS             complement(3880..4578)
                     /locus_tag="VW325_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735220.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFLEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            complement(4588..5340)
                     /locus_tag="VW325_001883"
     CDS             complement(4588..5340)
                     /locus_tag="VW325_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735221.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(5340..6104)
                     /locus_tag="VW325_001884"
     CDS             complement(5340..6104)
                     /locus_tag="VW325_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735222.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(6200..6605)
                     /locus_tag="VW325_001885"
                     /pseudo
     CDS             complement(6200..6605)
                     /locus_tag="VW325_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6785..7417)
                     /locus_tag="VW325_001886"
     CDS             complement(6785..7417)
                     /locus_tag="VW325_001886"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9735223.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKDDQENWQEG"
     gene            complement(7451..8152)
                     /gene="pyrF"
                     /locus_tag="VW325_001887"
     CDS             complement(7451..8152)
                     /gene="pyrF"
                     /locus_tag="VW325_001887"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9735224.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            8473..8565
                     /locus_tag="VW325_001888"
                     /pseudo
     CDS             8473..8565
                     /locus_tag="VW325_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(8887..9600)
                     /locus_tag="VW325_001889"
     CDS             complement(8887..9600)
                     /locus_tag="VW325_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9735225.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(9593..10426)
                     /locus_tag="VW325_001890"
     CDS             complement(9593..10426)
                     /locus_tag="VW325_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9735226.1"
                     /translation="MIEVSHLSKSFGDKIALNDISFTVKEGQIFGFLGPSGSGKTTTI
                     NILTGQLLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMSLYNNLLFYSKFY
                     NISKLRVDNLLKRVGLYDSCKMVAGKLSTGMRQRMLLARALINKPAVLFLDEPTSGLD
                     PTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKLVEQGAPSELIQR
                     YNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITLTGGKLN
                     A"
BASE COUNT         3493 a         2167 c         1736 g         3187 t
ORIGIN      
        1 cctccttaat aagtaagcgt ttactttgtg tattacaaaa atatcttaac tctttttcaa
       61 aacttttaaa atattttgtt tggaaatttc agaaatttta tgtctatgat aaaaatcctt
      121 ataacggcaa taaaaaatag atattatcca aagaagattt taagtgctac aataactgta
      181 ttatttctag atgggaggtt ctatttttgg attgatccat tgttgaacaa tatctaccac
      241 tatatcaaaa ggcattcttt ctgaccttgc atattgcagt ttggggaatt ttgggatcct
      301 ttctgctcgg tttaatcgtt agtatcatcc gacattatcg aatccttgtt ttgtcgcaag
      361 tagcgacagc ctacattgaa ttgtcacgta atacgcccct tttgattcaa ctcttctttc
      421 tctacttcgg tcttccccga atcgggattg tcctatcttc agaagtctgt gcaacgcttg
      481 ggcttgtctt tttaggagac tcctatatgg cagaatcttt ccgaagtggg ctggaagcca
      541 tcagtcaaac ccagcaggag attggcctcg ctattggtct gacacctcta caggtctttt
      601 actatgtggt tcttccgcaa gcaacagcgg tggcactccc ctcctttagt gccaatgtca
      661 ttttccttat caaggaaacc tctgttttct cagcagtggc tttggccgac ctcatgtacg
      721 tcgccaagga tttgattggt ctctactatg agacagacat tgcgctagct atgttggtag
      781 ttgcttatct gatcatgctg ctacccatct cactggtctt tagctggata gaaaggaggc
      841 tccgccatgc aggattcggg aatccaagta ctctttcaag gaaataatct cctgagaatc
      901 ttacagggat tgggcgttac gattgggata tccatcctgt ctgtcctctt atccatgatg
      961 ttcggaacag tcatgggaat catcatgacc tcccattcta gaatcatacg atttttaaca
     1021 cgattgtatc tggaatttat ccgtatcatg ccccagctgg tgctactctt catcgtttac
     1081 tttggcttgg ctcgaaactt taatatcaat atctcaggtg agacttcagc tattatcgtt
     1141 tttaccctct ggggaacagc tgaaatggga gacttggtac gtggagctat cacttctctc
     1201 cctaaacatc agtttgaaag tggacaggca ctcggcttga ctaatgttca actttactac
     1261 cacatcatca tcccacaagt cttgagaaga ctgctaccgc aggctatcaa tcttgtcact
     1321 cggatgatta aaaccacttc attagttgtt ttgattgggg ttgtggaagt gaccaaagtt
     1381 ggacaacaaa tcatcgatag caatcgcctg accatcccaa ctgcttcatt ttggatttat
     1441 ggaaccattc tagtcttata tttcgcagtt tgctacccta tttccaaact atccactcac
     1501 ttagaaaaac attggagaaa ctaaatgtct gaaactatct tagaaatcaa ggaactaaaa
     1561 aaatccttcg gagacaatcc catcctccaa ggactttctc tagaaatcaa aaaaggggaa
     1621 gttgttgtca tcctagggcc atctggttgt gggaaaagta ccctccttcg ttgcctcaac
     1681 ggcttagaaa gtattcaagg tggagatatt tttctggatg gtcagtctat cgttgaaaat
     1741 aaaaaaaatt ttcacctagt tcgccaaaag attggcatgg tctttcaaag ttatgaactc
     1801 tttccccatc tggatgtctt acaaaacctc atcctaggcc ctatcaaaac tcaaggaagg
     1861 gacaagaaag aagtaacgga agaagctttg caattactag atcgtgtcgg tttgctggat
     1921 aaacaacata gctttgcccg tcaattatct ggtggacaga agcaacgtgt tgcaattgtc
     1981 cgtgccctcc taatgcatcc agaaatcatc ctttttgacg aggtgactgc ttcgctggat
     2041 ccagaaatgg tgcgtgaggt gctggaactt atcaatgatt tggcccaaga aggccgtacc
     2101 atgattttag taacccacga aatgcagttt gcccaagcca ttgctgaccg gattatcttc
     2161 ctcgaccaag ggaaaatcgc tgaagaagga acagctcaag ccttctttac caatccgcaa
     2221 accaaacgag cccaggaatt tttaaacgtc tttgacttta gccaatttgg ctcatatcta
     2281 taaaggagat tcttatgaaa ctattcaaac cactcttaac tgttttagca cttgcctttg
     2341 cccttatctt tatcactgct tgtagctcag gtggaaacgc tggttcatcc tctggaaaaa
     2401 caactgccaa agctcgcact atcgatgaaa tcaaaaaaag gcggtgaact gcgaatcgcc
     2461 gtgtttggag ataaaaaacc gtttggctac gttgacaatg atggttctta ccaaggctac
     2521 gatattgaac tagggaacca actagctaaa gaccttggtg tcaaggttaa atacatttca
     2581 gtcgatgctg ccaaccgtgc ggaatacttg atttcaaaca aggtagatat tactcttgct
     2641 aactttacag taactgacga acgtaagaaa caagttgatt ttgcccttcc atatatgaaa
     2701 gtttctctgg gtgtcgtatc acctaagact ggtctcatta cagacgtcaa acaacttgaa
     2761 ggtaaaacct taattgtcac aaaaggaacg actgctgaga cttattttga aaagaatcat
     2821 ccagaaatca aactccaaaa atacgaccaa tacagtgact cttaccaagc tcttcttgac
     2881 ggacgtggag atgccttttc aactgacaat acggaagttc tagcttgggc gcttgaaaat
     2941 aaaggatttg aagtaggaat tacttccctc ggtgatcccg ataccattgc ggcagcagtt
     3001 caaaaaggca accaagaatt gctagacttc atcaataaag atattgaaaa attaggcaag
     3061 gaaaacttct tccacaaggc ctatgaaaaa acacttcacc caacctacgg tgacgctgct
     3121 aaagcagatg acctggttgt tgaaggtggc cattaactct taaaagggac tggattttaa
     3181 gctccaatcc ctttttaaga ttttacctat aacatcctga gtctatctaa gatgttcaat
     3241 ctgaacacag tgtacatact ttatcttcta ttgcatatac tttatcacat aagatacgaa
     3301 tatcctcttc actatgacta gcaatcaaaa ttgttgtccc tttttcacta gagagctttc
     3361 taaacaatgt tctcatattt tctacacttg atttatccaa ggcattcata ggttcatcta
     3421 gtaaaagaat agagggattc tccataattg cttgagcaat ccctagcttt ttcctcatac
     3481 ctagcgaata agttttaact ttctggtctt tttgctcata tagaccaact attttcagtg
     3541 tatcattgat ttcctgatta ccaactactc ctcgtatgct tgccaaatat tgtaaattct
     3601 taaagccact ataataattt ataaaaccag gttcttcaat caaagctccc aaattagctg
     3661 gaatttttct ctcaggaaca atattttccc cattgattaa cacttctcca taagacggac
     3721 tatataaacc agctattaat ttaaacaata cacttttccc tgagccattc gcaccagtaa
     3781 ttcctataat ttccccctgt ttacaactaa aattaaggtt ttgaaaaaca catgtttttt
     3841 ttaatttcaa ctcaatattt tttaatatga ttatttcatt cattctataa acctcctctt
     3901 ttgacgagtg aaatagaaaa tacttttaaa aagaaagact aaaaatagca actgaagaaa
     3961 taaatctcgt cctatatctc cattccctcg attcaaaata taaaatagat aattagttcg
     4021 atttcctaca aatagaccac caaacacaat catgagtaaa aagaaactaa cgcaagcaaa
     4081 gttcatttgt acattcgctc ctagtgcgta taacataaag caaaacaaga ctaacacaca
     4141 agcattcaaa gaatcaacta taaacaaaac tcctatatcc cagtttggta actgtaacat
     4201 aaattgaatc actagaactt ttaccatgat taaaatgagt ccataaagaa aacaggtcca
     4261 aatctgatac ctaatccaga gcttaataga agaaaatcga atcacttcca gaaagaaatc
     4321 ggatgctctt ctacgatata caactgctag aaaaaatgta tgaataataa accaatgaag
     4381 caaccatata gataagacta aaaaagagtt ttgactcaga ttaggccctt ttaaaatttc
     4441 tataacaagt ctatcatttc cttctaacca ataccataat cctaaacaaa tcaatatcca
     4501 aaaaagaaca taaccccaaa agtacttggt atagattatc tgattcaacc tccacatgtg
     4561 actacttaac tttcccattt ccaactttta cactctcctc cagtttctat gaacagaaaa
     4621 tacagtcaat ggaattatga tagatagtaa tgtaagccaa taaatcaatg catacggatt
     4681 ggaatttacc atatactgaa aaattggcaa taatccaatt atcccattgt ccaactgata
     4741 aatccagaat aaggattcta caaataaaaa agtaaacaga aatactaagc tcatgtttga
     4801 ttttttaaac caagaaaaac tgctaatagt aagtaaggat aaaaagaaat agtatgctat
     4861 ataagagaaa aaaaatccta taaagaaact agcattgttt gcaatactta taccataaaa
     4921 ttctcttaaa aaatcaacct cctttatctc caaagagaag ctaaaaccat tactaaatgc
     4981 aattagaaaa atcaataaaa ataaagtcgc cgtccaaatc cccgtactaa gagctgctaa
     5041 tttgaaacta aaactggtaa agtgcttaat tgatttcaga cgaatgcgac actccaacct
     5101 attaaaatag ttattcatca aataaaaaaa gaacaatata tatgtgaacg gaaagcaata
     5161 tactccagtc gtcatatctt gaagtaaaac taagatccat tctaatacat ttggatggat
     5221 tgaatattgg cgacagcgca ataaatatac tgtactagat aaaacacagg atagcagtaa
     5281 tataaaataa accaatactg ataaaaaatc ttttttgtaa aattgaacaa attgtttcat
     5341 tatacgtagt cctctgaatg tagaaaaaat gtaccataaa caaccaaaca actaacaaat
     5401 aaaataaaag caagatgccc actaactaag gaaagactga tatctttctg atatcccaaa
     5461 gctaatgttg tcacaggttc taagtaagat agccctaaaa tagcccaaaa aataccacca
     5521 accatcatat aggcaactgg gatgaaaata gctcctattt ttttcttcac tagcaaagca
     5581 ctagctagtc caaaaataga gaacacagcg ccccaaactc cataccagag agtcgtcaca
     5641 agactataga gcaactgatt agaatcaaat aattctttta aggcaccact ataatctcca
     5701 atataaattt cctgataagg agtcactaaa agattaattc ctaataataa taaatagggg
     5761 agaaaaaaga ctagaaaaga agaaataatt gcagtactac ccacaatagc tagatacctc
     5821 tttttagaaa ttcggcacaa ttgtgctgtt agaaaatgac tctcagcatc ctctattatc
     5881 tgactagaat agggcagtgt acagataagt gcagctacta ggctaatcgg tgaaaatcct
     5941 cgaatagaag aagcggcaaa aaaaatcgat aaaccttcaa ttttataaat accattgaaa
     6001 gcaaggaaat ttcctaaact catgcaaaga agggtcaaaa ataaagacat ataaaatcga
     6061 ggtgattgaa cgactcgtac aagattacaa atgaaaaatt ccatccttac tcctccttat
     6121 aataaaaata gggtgtagca ttcttttttc atgctacacc cacaatcaac catctttaag
     6181 gcttactctg acaagtaagt taataagaat ctggactcca agaacctgaa gtatgaattc
     6241 ttacatgatt tccaaattgt ggcgccatag ctaatctagt accagaacca atataattgt
     6301 caccacctcc attatagtac atgacaatcc tagagccaga ccccaatgaa tataccgggg
     6361 taatatctga cccactatag gcgcgctacg aatagaggta tttaaccttt taccgccacc
     6421 agtgctgtca ctattattaa ttccagcaga ggcgttttct ttctcaagct cactagttgt
     6481 atccgaaccc caacgcggca acgttaaatc ataatttcca atctgtctag ctaaaactgt
     6541 agggagaaac aaactagatg caattgtaac tacgcaaaaa aggaatatat ttttcttctt
     6601 ttcatgacat cgtcctctta agaagtgata aacaagatag ttgattgatc aatttgatac
     6661 atattacatt ttcattatat caataaaaga aagcgttgtc aattatttta tttaaaatat
     6721 tgtcatattt taaataaaat ggtaaaagag gctcggtagc ctcgtctctc tttatcttac
     6781 tgacctaacc ttcttgccaa ttttcttggt cgtctttaaa gcgttttaga agatcaaggc
     6841 cctctggcgt gatgtaacct tcttcttggg ctagatggat aagctcgcta tagtttgaaa
     6901 gcgtcacaag tttaacacca gcatctgcaa agttcttatc tgcttttggc aattggtagc
     6961 tgaaaatcgc tacaactcca agcacatctg ctccttctcg cttggctgct gctacagctt
     7021 caagaactga accacccgtt gaaataaggt cttcaaccac taccattttt tgaccttgag
     7081 ctacgcgacc ttcgatttga ttaccagctc catggtcttt tggtttgcta cggatgtagg
     7141 caaaaggcaa gtccatctta tcagcaataa tggctccgtg tggaatccct gctgttgcag
     7201 ttcctgcaat cacttctact tcaggaaagg cttctttgat agcttccaca aaaccatttt
     7261 caattagggt acgagtttct ggataggcta gtgtcacacg attatcagtg taaatcggtg
     7321 acttgatacc agatgcccaa gtgaagggtt cctctggttt gaggtaaacg gcttggattt
     7381 tcaagaggtg gctagcgata tctttagcaa gtgtcatggt attctccttt tatttttcta
     7441 atctagttct ttaattccag tcctgtgtcc attcatcctt gatggcatga taagctgcaa
     7501 caggatcctc agcttgggta atgggacgtc ccactacgat atagtcactg ccgatttgat
     7561 aggcatcagc aggtgtcatg actcgttttt gatctccaac tgcaacacca gctggacgaa
     7621 tccctggtgt cagacagata aaatctggat tggtagcctg cttgatgact tgtacttcct
     7681 gagccgagca aacaacacca tccaagccag cttcagctgt cttcttggca tagtgaatca
     7741 cagactcttg cagactggtt tggatatttt gaaactcctg catctgagct tctgacgttg
     7801 atgtgagctg agttacagcg atcaatttgg cttgacttcc aagaccttca cgcgcagcct
     7861 tcatcatctc tacaccacca gccgcatgaa cattggtcat atccacacca agctgagata
     7921 ggatcttcat tgctgacttg actgtattag gaatgtcatg aagtttgaga tccaaaaaga
     7981 cactatgacc caaacctttt aagtaggaca caatctcagg ccccgctgcg taataaagct
     8041 ccatccctac cttgagataa aggctttctt ctgctgggaa aagagctaaa aattccttga
     8101 ccgcctcaaa actaggaaaa tcaagagcaa tgattggacg atgttctcgc ataggtttct
     8161 ccttttacct ttactagtga gagagtctgg ccaacagaag ccacgaaaaa aggaacctgc
     8221 caacagcaag gttccacgaa aaatgggtag tctccaccct ttcaccgtct aaccttagct
     8281 gcctctctgg actgctttaa aggtttttta ctattctatt atttctacta gcacttgtca
     8341 agtaaaaaca tggtcacgta tgtgttgact gctattgaac acctcacaag actctttcca
     8401 aaagaatagt taaagacttt cacttaggac agagaaaaag agtttgcttg ttacccttta
     8461 attgaacaaa tgggaattct gttttatttt gcggatgcct attcatcttg gcaaagggga
     8521 agtaatgaga atgcgaatgg acttcttcgt gagtattttc ttaaaccgat ttggcaacaa
     8581 ttaccgaaaa aatccttaat ggtgctttgc tagcttatta atcaccgtcc ccaaaaatac
     8641 ttggattaca agacaggttt tgaagcactt atagaggaga tttaaaaatg ttgcattttt
     8701 tattgcaatt tatcagttat cttatcaccc aaaacttctg caattagtat gagcactatt
     8761 gttatttttc ttttttcgta cactcaaatc aaaatatagc aataagacta ttctgtagaa
     8821 ttgctgtcaa aaagatcagt tcatttttcc ttcccacttg tgtttataat cattaaaata
     8881 actcaattaa gaaattttct ttttcctaat agttatcgta attaaagtta atagaagaac
     8941 aatccaaata agtagtgtta gattaggaat tagagtttca ttccatgaaa atccctccca
     9001 ttttgtgaaa aacttagtaa acagtcccat aaaactataa tctgttatct tcgcaactgt
     9061 cttatccaaa ccagatagca tcggtaagaa agcaactaaa atcatagcag gaagactgat
     9121 aacctgagct actacttggc tcttcgcggt taaacctatc aataaataga ataaaatgat
     9181 ggataaagag gttagaagaa cgattgtaat ataattaaaa gtatgtacaa ttgtaactcc
     9241 taaaataaga ggagtagttc ccataatcac aaaagttagc aaaaaaggaa gaaacatcgt
     9301 tgataaaatg tattcggatc ctttaacacc actcaacaga agagtttgta aattgtactt
     9361 ttctttttct tcagacaaga taatagttat aggacttcca acagccaaag ccaaagaaaa
     9421 aggtaaacac atcatcaaaa gaactaatgc ctgttgatcg ttgaccttcc cctgtgtttc
     9481 cataagatat ttataaaaat atgtgaaagc aaaaggcact aaaacttgca ataaaatact
     9541 cttattggaa aggataattt gacaacgcaa ccataccaaa gccagaaacc gtttaagcat
     9601 ttagctttcc tcctgttaat gtgataaaaa tatcttctaa agtaggctca catgaatgaa
     9661 ttgaaaaaat attttccaga tcagcctgag atacctgttc tagtgatgta aaatcaaaag
     9721 ttatctgatt cccattataa tctgtaacct taatcttttt atctttatta tatctttgaa
     9781 tgagttcaga aggagctcct tgctcaacta atttcccttt atttaataag gcaacataat
     9841 cacataaaag agttgcttca ttcatatcat gagtcgttag aaaaatcgtt gtccctgctg
     9901 ttttcaattc taaaattaac tcatgaattg ttcgagaagt tgtgggatct agacctgagg
     9961 tcggttcatc cagaaagagt acagcgggtt tgttgataag agctcgtgct aaaagcattc
    10021 gttgcctcat tccagtggat aattttcctg ctaccatctt gcaactatca tataatccta
    10081 ctcgctttaa caaattatca acacgtaatt tactaatatt ataaaattta ctataaaaaa
    10141 gaagattgtt atacagagac attttctcat aaaatccact tgtatcgcta accaatccaa
    10201 ttctctttaa ttcaccgctt gttaaatttt gagatttttg tcccaaaata atagattgtc
    10261 ctttatcggc aaggagctgc ccagtcagaa tattaatcgt tgtggtcttt ccagaaccag
    10321 atggtcctaa aaatccaaaa atctgacctt ctttaacagt gaaacttata tcatttaaag
    10381 ctattttatc accaaaactt tttgataaat ggctaacttc aatcatactc attgcccctc
    10441 ctttatattc tacattttta cttaacatct atttacttag ctttatcaaa taaacgtctt
    10501 aaaaaaaaca ataacattaa gcatataacc gttagcaaga ttgtttcaat attattgata
    10561 atatgataca ttctcaagca gta
//
LOCUS       DAVKLX010000065        10535 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKLX010000065 DAVKLX010000000
VERSION     DAVKLX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.065"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..1754
                     /locus_tag="VW325_001891"
     CDS             <1..1754
                     /locus_tag="VW325_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9735227.1"
                     /translation="AKNYKTNNLFDALEKYRKAFVPDKTNNEWFKEQTKAYIVEEKST
                     IKEVSDKQSIAGSPYSIGVYDRLTSPSWKYPSMVLPLLTLPEKSVFIIANISTIGFGA
                     YDRYRSKEHPAGTDLNDYVEKKAKEAAVRFRDHYDYWYRILDDKNKEKLYRSVLVYDA
                     FRFGTDEKEDKDTYQATFETNHPAIKHFFGPAGNNVVHNSNGAYATGDAFYYMAYRML
                     DKDGAVTYTHEMTHNSDREIYLGGYGRRNGLGPEFYAKGLLQAPDHPNDPTVTINSIL
                     KYDQSEESTRLQVADPTQRFGSVDDLNKYMHNMFDVIYMLEILEGKAVAKLDTNQKYD
                     LLRKIENEYKPDPDGNSVYATNVVRRLKPEELTKLTTFNSLIEHDIITRRGYVDEATY
                     KRNGYYTINLFSPIYSALSSKIGTPGDLMGRRIAFELLAAKGYKDGMVPYISNQYEKE
                     AKAQGKVITSYGKQIGLVTDEIVLSKVFNNQYNSWIDFKKDMYKEREDKFGKLNKVSF
                     IDPNGSWARQQKVTIDNINRLEKMIEDAVKFDAEDEVAKLYPETNSRVLKLKKAIFKA
                     YLDQTGDFRSSIFENKK"
     gene            1798..7011
                     /locus_tag="VW325_001892"
     CDS             1798..7011
                     /locus_tag="VW325_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9735228.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNVDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            7265..8116
                     /gene="ylqF"
                     /locus_tag="VW325_001893"
     CDS             7265..8116
                     /gene="ylqF"
                     /locus_tag="VW325_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9735229.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            8103..8882
                     /locus_tag="VW325_001894"
     CDS             8103..8882
                     /locus_tag="VW325_001894"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9735230.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            8898..10445
                     /locus_tag="VW325_001895"
                     /pseudo
     CDS             8898..10445
                     /locus_tag="VW325_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
BASE COUNT         3603 a         1730 c         2153 g         3049 t
ORIGIN      
        1 tagcgaaaaa ttataagact aataatttat ttgatgcatt agagaaatat agaaaagcat
       61 ttgtaccaga taaaacaaat aatgagtggt ttaaagaaca aactaaagca tatattgtag
      121 aagaaaaatc tactattaaa gaggtaagtg ataagcaatc aatagctggt agtccatatt
      181 caattggagt atatgataga ttaactagcc catcttggaa atatccaagt atggtgttgc
      241 cgctattaac attacctgaa aaatcggtgt ttattatagc caacatttcg actataggtt
      301 tcggtgctta tgatagatat agaagtaaag aacatccagc aggaactgat ttaaatgact
      361 atgttgagaa aaaagctaaa gaagcggctg ttagatttag agatcactat gattattggt
      421 atagaatact agatgataaa aataaagaaa aattatatag aagtgttcta gtctatgatg
      481 catttagatt tggtacagat gaaaaagaag ataaagatac atatcaagca acttttgaaa
      541 ctaatcatcc agctataaaa catttcttcg gtccagcggg aaataatgtt gttcataatt
      601 ctaatggagc atatgctaca ggggatgcat tctattatat ggcatatcgc atgcttgata
      661 aagatggtgc ggtaacttat acacacgaaa tgacacataa ctcagataga gaaatttatc
      721 ttggaggcta tggaagacga aatggacttg gaccagagtt ttatgctaaa ggactgctac
      781 aagctcctga tcatcctaat gatccaacag tcactattaa ttccatatta aaatatgatc
      841 aatcagaaga atcaacaagg cttcaagtag cagatccaac tcaacgattt ggaagtgttg
      901 atgatctaaa taagtatatg cataatatgt ttgatgtaat atacatgctt gaaatattag
      961 aaggtaaagc tgtagctaaa ttagatacaa atcaaaaata tgatttatta agaaaaatag
     1021 aaaatgaata taaacctgat cctgatggaa attctgttta tgcaacgaat gttgtacgta
     1081 gattaaaacc agaagaactt actaaattaa ctacatttaa cagtttgata gaacatgata
     1141 ttataacaag aagaggttat gtagatgaag caacttataa gcggaatggc tactatacaa
     1201 ttaatctatt ctcaccaatt tattcagcat tgagcagtaa aataggaaca ccaggagatt
     1261 taatgggacg acgtattgca tttgagttat tggcagccaa ggggtacaaa gacggtatgg
     1321 ttccatatat ttctaatcag tatgaaaaag aagcgaaagc tcaaggaaaa gttattacat
     1381 catatggtaa acaaataggt ttagttactg atgaaattgt tctttcaaaa gtatttaata
     1441 accagtataa ttcttggata gatttcaaga aagatatgta taaagaaaga gaagataaat
     1501 ttggaaagtt aaataaagtt agttttatcg atccaaacgg ttcttgggct cgacaacaga
     1561 aagtgactat tgataatatt aatagacttg aaaaaatgat tgaagatgca gtaaaatttg
     1621 atgctgagga tgaagttgcc aaattatatc ctgagacaaa tagtagagtt cttaaactga
     1681 aaaaagcaat ctttaaagct tatcttgacc aaaccggtga ttttagaagt tcaatttttg
     1741 agaataaaaa atagtgttta ctattaggaa ataaaaagtg aaggtggagg attagaattg
     1801 tcattattaa aaaaagataa attttccatt cggaaaataa agggtattgt tggttcggta
     1861 ttcttgggga gtcttttatt tgcaccgtca gttgttggtg catcgactta tcattactta
     1921 gattatagta gtttgacaca aactgaacgt gatcaactca aacaaggtag acctgatgaa
     1981 tccaaagagt catatgcttt ggattatgaa aaagacgcac taccaaacac aggaagttct
     2041 caatctatta tgactgcatt gggtttgtta gctatcggga gtcttattgt gattatcaca
     2101 aaagataata gaaataaaaa aatagctaca tttttgattg taggggcgac aggtttagtt
     2161 acactatcaa ctgcttcagc acttaatttg aatgctaaca tccatgagtc tggacgtgat
     2221 ggtgtgttgc aaatttctgg ttacagatat gttggttact tggagcttga tgataaaact
     2281 gtttcgtcgg tttctccagc atcaactgtt tcgccagttg agcaacccaa agttgtaaca
     2341 gagaaaggcg aaccggaagt tcatgagaag cctgactata cgcaaccaat aggtgcaaac
     2401 cttgttgagc ctgaagttca tgagaaacta gcatacactg aaccagtagg gacaacagga
     2461 gtagatgaaa atggaaactt gattgaacca cctgttaacg acatcccaga atatactgag
     2521 ccagtaggta caacaggagt ggatgaaaat ggaaacttga ttgagccacc tgttagcgac
     2581 attccagagt atactgaacc aatatctaca gtttcagaag ttgcatcaga aagagaagag
     2641 ttgccttctt tacataccga tattcggaca gagactattc ctaaaaccac tatagaagaa
     2701 tctgatccta gcaaatttat aggcgatgat tctgttagac aagttggtga ggatggcgaa
     2761 cgtcaaattg ttactagcta tgaagagtta catggcaaaa aaatcagtga cccagttgaa
     2821 acagtaacta ttttgaaaga aatgaagcct aaaattcttg taaaaggtac caaagaaaaa
     2881 cctaaagaaa aaacggctcc tgttttgacc ttagatagaa caaatacaaa tgtattaaac
     2941 cgatccgcaa ctctttctta tcatttagtt aatacagatg gagtcacaat caataaaatt
     3001 actgcgacaa ttaaagatgg aaatgaaatt gtcaaaacag tagatttgac ttcagagcaa
     3061 ttagataagc aggtagaaga tttaaaattc tacaaggact ataagataga gaccactatg
     3121 acctatgacc gtggcaaggg agaagaaact gcaacattag aagagaaacc acttcgcttg
     3181 gatttgaaaa aagttgaaat taaaaatatc gcatctacta atttagtgaa ggtaaatgac
     3241 gatggtactg aaacacctag tgattttatg actgaaaaac cttcagatga agatgtgaag
     3301 aaaatgtact taaaaattac tagtcgtgat aataaagtaa cacgtttagc agttgataaa
     3361 attgaagaag tgactgaaga aggtaagaaa ctttataaaa tcactgccga agctcaagat
     3421 ttgattcaac atacagaccc aacgaaggtt cgtaacaagt atgttcatta tattgaaaaa
     3481 ccagttccaa aagttgatga cgtttattat aactttaaag agttagtaga tgccatgaat
     3541 gctgataaaa atggtacatt taaaatcgga gcagatttga atgcaacgaa tgtaccaaca
     3601 cctaacaaac aatatgttcc aggtacgttt aagggacatc tatcaagtgt tgatggtaaa
     3661 caatatacaa ttcataacat tgcccgtccc ttatttgacc gtgttgaaaa tggttctgtg
     3721 aaaaatatta atttaggtaa tgtggatatc aatatgcctt gggctgacgg aatagcacct
     3781 gttgccaata tggtgaaaaa tgcaactgtt gaagatgtta aggtaactgg taatgtagtt
     3841 gctaataaca atattgcggg tatcgtcaat aaaatcgata gtggtggtca gttgacaaat
     3901 gtagccttca tcggtaatct tacaggtgtt ggtgataaag gtcaatatat ggctggcatt
     3961 gccggtgaga tttggagagg taatcttgca aaagcatatg tcgaggctga tattgttgct
     4021 aacagagccc gtataggtgg tttagttgct aaaacagata atggtaacga ctctatggga
     4081 attggtaaat acggttcaat tcgtaaatca gtcaccaaag gaaccattaa gactaaagtt
     4141 ttgtttgaaa caggtggatt tatcaatagc aatcttcctt ttggtaaact tgaagataat
     4201 atttcaatga tgagagttga aaatggtgaa gagttctttg gttcttccga tttggactac
     4261 gatggtggtt atttcacaaa tggctggtta gagcgtaact ttgttgtcaa aggtgtcagt
     4321 tcaggtaagc attcatacaa acgttctcgt gacaaaatta aagaaattag tcaagatgaa
     4381 gctaataaga gaattgcaaa ttttggactt actgctgata aatacgaaat caacgaacct
     4441 gtggtcaatc gcttaaatcg tttaaccaga agagaagatg aatataagtc aactcaagac
     4501 tacaaatccg aacgtgattt agcttatcgt aatattgaga aacttcaacc attttataat
     4561 aaagaatgga ttgtcaacca aggtaacaaa cttgcagaag attcaaactt agctaagaaa
     4621 gaagtccttt ctgttacggg gatgaaggat ggtcaattcg tgaccgattt atcagatatt
     4681 gataagatta tggttcacta tgctgatggt actaaggaag aaatggatgt tactaagaat
     4741 actgattcta aagtccaaca agtgcgtgaa tatagtgtat ctggtcttgg tgatgttgtt
     4801 tatacaccga atatggttgt taaaaatcgt gataaattga ttgctgatgt taaatctcaa
     4861 ttatcaagtg tggaattgat ttcacaagaa gtaagagatt taatgagtag acgtgataaa
     4921 cctgcagaga atacggatga acgtaagaat ggttatatta aagatctata cttagaagaa
     4981 agctttgccg aagtgaagca aaatctagat aagttagtca aatccttggt cgaaaatgaa
     5041 gaccatcaat tgaatggtga tgaagctgcg ataaaatcac ttctcaagaa agtggaaacc
     5101 aataaagcta aaatcatgat ggctctgact tatctcaatc gttactatga tatcaaatac
     5161 ggtgacataa gtataaaaaa catcatgatg ttcaaacctg atttttatgg taaaacacca
     5221 agtgttattg acagactcat caacatcggt tcaagtgaaa agaacttgaa aggggatcgg
     5281 actcaggatg cttaccgtga aatcatcgca ggtaatactg gtaaaagtaa tttgcgtaac
     5341 ttcttagaat acaacatgcg tctgtttaca gaggataaag acatcaatga ttggtttatc
     5401 cactcagcta agaatgttta tgtttcagaa cctaagacaa caaatacaga gttgaaagat
     5461 aaacgtcatc gtgtgttcga tggtttagat aacggtgttc atggtcgtat gattttacca
     5521 cttctaacgc ttaaagatgc ccatatgttc ttaatttcaa catataacac gatggcgtac
     5581 agttcatttg agaaatatgg aaaacataca gaagaagctc gtaatgagtt caaaactaag
     5641 attgatgaag ttgcacatgc tcaacaaact tatttagatt tctggtccag attagctctg
     5701 cctaatgttc gtgatagact tcttaagagt caaaatatgg taccgacacc agtttgggat
     5761 aaccagacct acaatggttc tcctgtaggt cgtcgaggat ttgacagtaa aggtaaccct
     5821 atagctccaa ttcgtgaatt atatggtcca acatggcgac accatgatag agactggcgt
     5881 atgggcgcta tggcatcaat attccctaat ccaaataatg atgacaaggt cttgtttatg
     5941 gtgacggata tgattagccc atttggtatt tcagccttta ctcacgaaac aacacacgta
     6001 aacgatagaa tgctttactt cggtggtcat aagcatcgtc aaggtacaga cgttgaagcc
     6061 tatgctcaag gtatgttgca aacacctgac agctcaacga ctaatggtga atacggcgca
     6121 cttggaatta acatggctta ccatcgtcca aatgatggta accaatggta caacccagat
     6181 cctgacaaat taaagacacg tgatgacatt gaccgctaca tgagaaacta caatgaagct
     6241 atgatgttgc ttgatcatgt agaagctgat gctgtacttc ctaaaattaa aggagacaat
     6301 agtaagtggt tcaagaagat tgacaaagaa atgcgctcta agattcagta taatgatctt
     6361 ttaggtccaa accaatggga tagtatccgt gatttgaaag atgaagaaaa ggtaatgact
     6421 ttatctagcg tcaatgattt agtggataat aacttcatga caaaacatgg aaatccaggt
     6481 aatggacgtt atagaccaga agattttaca ccaaactcag cttacgtaaa tgtcaatatg
     6541 atggcaggaa tttacggtgg gaatacgagt caaggtgctc ctggttcatt atcatttaag
     6601 cacaatgctt tccgtatgtg gggctattat ggttatgaaa atggctttat tagttatgta
     6661 tcaaataaat acaaagcaga agcagataaa aacaaccacg gtttactaag cgataagcta
     6721 ataattaata aagtatcgaa aggaaacttt aatactcttg aagagtggaa gagacattgg
     6781 tacggggaag ttcttgctaa agctaagaaa ggttttgagg ctatcgatat tgatggcgtc
     6841 catatcagta actatgatga gttaaggcct ttatttgata aagcagttga agaagatttg
     6901 aagaaaccag atgacttctc tcacactgtt gctcttaaat ctaaggtctt caaagccctt
     6961 cttaaaaaca cagacggttt ctttaataaa ctgtttaaag aagacattta aatgtttaga
     7021 atatagaagg aacagttttt tgctccctct gaaaagtcat catttgatgg cttttttcta
     7081 tatagggtac ttgaaaggct atcatcggac aaaataaaga aggcatgata taatataagg
     7141 tagatttcta tgtcataaaa caaaaaactg tttgaacatc attcatttga gaacccttta
     7201 tgttcaaacg atagtaaaat aaaataggat ctctaaaccc ttgctacgaa aggaaaaaaa
     7261 ctcaatggct actattcaat ggtttcctgg tcacatgtct aaagcgcgtc gacaggtgca
     7321 ggaaaattta aaatttgttg attttgtaac gattttggtg gatgcacgct tacctctatc
     7381 tagtcaaaat cctatgttga ccaagattgt tggtgataaa ccaaaactct tgattttaaa
     7441 taaggcggat ttggctgatc cagcaatgac caaggaatgg cgtcagtatt ttgaatcaca
     7501 aggaatccaa actctggcta tcaactccaa ggagcaagtg actgtaaaag ttgtaacaga
     7561 tgcggccaag aagctcatgg ctgataagat tgctcgccag aaagaacgtg ggattcagat
     7621 tgaaaccttg cgtactatga ttatcgggat tccaaacgct ggtaaatcaa ctctgatgaa
     7681 ccgtttggct ggtaaaaaga ttgctgttgt tggaaacaag ccaggggtca caaaaggtca
     7741 acaatggctt aaaaccaata aagacctgga aatcttggat acaccgggga ttctctggcc
     7801 taagtttgag gatgaaactg ttgcacttaa gttggcattg actggagcta tcaaggatca
     7861 gttgcttcct atggatgagg ttaccatttt tggtatcaat tatttcaaag aacattatcc
     7921 agaaaagctg gctgaacgct tcaaacaaat gaaaattgaa gaagaagcgc ctgtgattat
     7981 tatggatatg acccgcgccc tcggtttccg tgatgactat gaccgttttt acagtctctt
     8041 cgtgaaggaa gtccgtgatg gcaaactcgg taactatacc ttagatacat tggaagacct
     8101 cgatggcaac gattaaagaa atcaaagaat tccttgtgac agtcaaggag ttagaaagcc
     8161 ctattttttt agagcttgaa aaggataatc gctcaggagt tcaaaaggaa atcagcaagc
     8221 gtaaaagagc cattcaagct gaattagatg aaaatttgcg cttggaatcc atgctttctt
     8281 atgaaaaaga actttataag caaggattga ccttaattgt aggtattgat gaggttggtc
     8341 gtggtcctct tgctggtcct gtagtcgctg cggccgtcat tttacctaaa aattgtaaga
     8401 ttaaaggtct caacgacagc aagaaaattc ctaaaaagaa acatctggag attttccaag
     8461 ccgttcaaga ccaagccttg tcgattggaa ttggtatcat agataatcag gtcatcgacc
     8521 aagtcaacat ctatgaagca accaaactag ccatgcaaga agcaatctcc cagctcagcc
     8581 ctcagccaga gcaccttttg attgatgcca tgaaactgga cttgcccatt tcacaaacct
     8641 ccattatcaa aggagatgcc aactccctct ctatcgcagc agcatctata gtagccaagg
     8701 taacacgtga tgaattgatg aaggaatacg atcagcagtt ccctggctat gatttcgcta
     8761 ctaatgcagg atatggcaca gctaaacatc tggaaggcct cacaaaacta ggagttaccc
     8821 caattcaccg aaccagcttt gaacccgtta aatcactggt tttaggtaaa aaagaaagtt
     8881 aattgaaagg aaataccatg gaggaacagt cggaaatagt ccgttctaag aaagaattcg
     8941 cctttgcatc cagcactata ctatcccaag ttggtcgagg aatcattgtc ggcctcatcg
     9001 ttggaattat cgtcggatcc tttcgtttct taattgaaaa gggcttccac ctgatacaag
     9061 gagtttatca agatcaaggg tacttagtgc gcaatctttt tgtactggtt ttgttttata
     9121 tactcatctg ttggctcagt gccaaactaa cacggtcaga aaaagatatt aaaggctcag
     9181 gaattcctca agtcgaagcc gaactgaaag gcctcatgtc cctcaactgg tggggcattc
     9241 tttggaaaaa atatgtgcta ggtattcttg ctattgccag tggactcatg ctgggtcgag
     9301 agggacccag tattcaactt ggagcagttg gtggtaaagg aattgccaag tggctcaaat
     9361 ccagtccagt agaggaacgt tccttgattg ccagtggagc tgcagcaggt ttagccgcag
     9421 cctttaatgc tcctattgca gcacttctct ttgttgtaga agaagtctat caccattttt
     9481 cgcgcttttt ctgggtctca actctagcag ccagcatcgt agcaaacttt gtgtctctac
     9541 tcatgttcgg tttgacacca gtattggata tgccagataa cattcctccc atgaccctag
     9601 atcagtattg gatatatctc gtcatgggaa ttttccttgg attttcaggt tttctctatg
     9661 agaaagctgt attaaacgtt ggaagagttt atgacttgat tggtcaaaaa atccatttgg
     9721 atagggctta ttatcccatc ttggctttta tccttatcat accagtcgga atcttcttac
     9781 ctcaaatcat tggtggcgga aatcagcttg tcctttcttt aactgaacaa aattttagtt
     9841 tccaagtttt attagcttac tttttaatcc gctttatttg gagtatgatt agctatggaa
     9901 gtggactgcc aggaggaatt ttcctcccca ttttagctct tggttctttg cttggtgcct
     9961 tagttggtgt tatctgtgtc aatcttggac ttgtcagtca agagcaattc cctatatttg
    10021 tcattctagg aatgagtggc tattttggag ccatatcaaa agctccctta accgctatga
    10081 tcctcgtaac tgagatggta ggagatattc gcaaccttat gccacttggt cttgtcactc
    10141 ttgtttctta tattatcatg gatttgctca aaggtacgcc agtctatgaa gccatgctgg
    10201 aaaaaatgct tccagaagaa gtatctagcg aaggagaagt tacacttatc gaaataccag
    10261 tttctgataa aattgctggg aaacaagttc atgaactcaa cttaccacac aacgtcctca
    10321 tcacaactca agtccataat ggcaagagcc aaacggttaa cggctcaacc agaatgtatc
    10381 tgggtgatat gattcacctg gttattccaa aaagtgaaat tggaaaagtc aaagatttgt
    10441 tgttgtatta tgatcattta cataatttat gttatgtaaa tgatcggttt gatttattta
    10501 gaaaaccgat tctcagaaat gagatcggtt atttt
//
LOCUS       DAVKLX010000066        10426 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKLX010000066 DAVKLX010000000
VERSION     DAVKLX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10426
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.066"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            1..389
                     /locus_tag="VW325_001896"
                     /pseudo
     CDS             1..389
                     /locus_tag="VW325_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            475..687
                     /locus_tag="VW325_001897"
                     /pseudo
     CDS             475..687
                     /locus_tag="VW325_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            587..1033
                     /locus_tag="VW325_001898"
     CDS             587..1033
                     /locus_tag="VW325_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9735231.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1171..2475)
                     /gene="eno"
                     /locus_tag="VW325_001899"
     CDS             complement(1171..2475)
                     /gene="eno"
                     /locus_tag="VW325_001899"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9735232.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2639..3085
                     /locus_tag="VW325_001900"
     CDS             2639..3085
                     /locus_tag="VW325_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9735233.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3082..3885)
                     /locus_tag="VW325_001901"
     CDS             complement(3082..3885)
                     /locus_tag="VW325_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU9735234.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQNFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3894..4223)
                     /locus_tag="VW325_001902"
     CDS             complement(3894..4223)
                     /locus_tag="VW325_001902"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU9735235.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4168..4539)
                     /locus_tag="VW325_001903"
     CDS             complement(4168..4539)
                     /locus_tag="VW325_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU9735236.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4598..6031)
                     /gene="glgA"
                     /locus_tag="VW325_001904"
     CDS             complement(4598..6031)
                     /gene="glgA"
                     /locus_tag="VW325_001904"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9735237.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6028..7167)
                     /gene="glgD"
                     /locus_tag="VW325_001905"
     CDS             complement(6028..7167)
                     /gene="glgD"
                     /locus_tag="VW325_001905"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9735238.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7157..8299)
                     /locus_tag="VW325_001906"
     CDS             complement(7157..8299)
                     /locus_tag="VW325_001906"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9735239.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTIKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8289..10217)
                     /gene="glgB"
                     /locus_tag="VW325_001907"
     CDS             complement(8289..10217)
                     /gene="glgB"
                     /locus_tag="VW325_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9735240.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3197 a         2288 c         1831 g         3110 t
ORIGIN      
        1 caccaagtaa aaggaacaaa accaagaaaa gttgatagag atagacttaa aaactatctt
       61 actgacaatc cagacgctta tttgactgaa atagcttctg aatttggctg tcatccaact
      121 accatccact atgcgctcaa agctatgggc tacactcgaa aaaaaagaac cacacctact
      181 atgaacaaga cccagaaaaa gtagccttat ttcttaagaa ttttaatagt ttaaagcacc
      241 tagcacaggt ctaacaaatg aatggtgagt taatcgctcc aatgacttac gaagagacga
      301 tgacgagcga cttttttgaa gcttggtttc agaaatttct cttaccaaca ttaaccgcac
      361 cattggttat tatattaaaa cgaagtaaga ataggacaaa tcgatcagga cagtaaaatc
      421 taattctaac aatgttttag aagtagaggt gtactattct agtttcaatc tactatatta
      481 atggacaatg caagattcca tagaatgggg aagctagaac tcttgtgtga agagtttggg
      541 cataaacttt tacctcttcc tccctactcg cctgagtaca atcttattga gaaaacatgg
      601 gctcatatca aaaagcacct caaaagggta ttaccaagtt gcaatacctt ttatgaggct
      661 cttttgtctt gttcttgttt caattgacta tataaattgt ctaagcgaaa caaccgataa
      721 gaattggcac aaaagcgacc gtatttttgt taccaataca ggaaaaccag ttcatagttc
      781 tatcttgagc aagtctctcc agcgagcaaa cgaacgcctt aaaaaaccaa ttcccaaaca
      841 tctgtcccct cacatcttca gacacaccac tattagcatc ttatcagaaa ataaaattcc
      901 tttaaaaaca atcacggaca gggttggtca ttccgactct gaagtcacta cttccatcta
      961 cacccacgtc acaaagaaca tgaaagatga agcaatcaat gtactggata aagttatgaa
     1021 aaagattttt taaaaagttt tgtccctttt ttgtcctcta aatacaaaaa tagcccttcg
     1081 gataaaatcc gaggggctag aaacgttgtt aaatcaacgg ccgaactttt gaatttcatg
     1141 gttcgggata aaatagttca ctgaactatt ttatttttta aggttgtaga atgatttcaa
     1201 tccacgatat tcagctactt caccaagttg gtcttcgata cgaagcaatt ggttgtattt
     1261 agcgatgcgg tctgtacgtg aaagtgaacc agtcttgatt tgtcctgcgt tagttgcaac
     1321 tgcaatatca gcgattgttg aatcttcagt ttcacctgaa cggtgtgata caacagcagt
     1381 gtaaccagct tctttagcca tttcgatagc ttcaaaagtt tcagtaagag taccgatttg
     1441 gttaactttg ataaggattg agttagcagc accttcttgg ataccacgtg caaggtagtc
     1501 agtgtttgtt acgaagaagt cgtcaccaac aagttgtact ttcttaccaa gacgttcagt
     1561 aagagctttc caaccatccc agtcgttttc atccatacca tcttcaatag tgatgattgg
     1621 gtatttgtta accaattctt caaggtagtc gatttgttct gcagatgtac gaacagcagc
     1681 accttcacct tcaaatttag tgtagtcgta aactttacgt tctttatcgt agaattctga
     1741 tgaagcacag tcaaatccga taaatacgtc tttacctggt acatatccag cagcttcaat
     1801 cgcagcaagg atagtttcaa caccatcttc agttccttcg aaacgaggag cgaatccacc
     1861 ttcgtcacct acggcagttt ccaaaccacg tgatttaagg attttcttaa gagcgtggaa
     1921 gatttcagca ccgtaacgaa gggcttcttt aaatgttggc gcaccaactg gcaagatcat
     1981 gaactcttgg aaagcgattg gagcgtcaga gtgagaacca ccgttgatga tgttcatcat
     2041 tggagttgga agaactttag tgttgaatcc accaaggtag ctgtaaagtg ggatttcaag
     2101 gtagtcagca gcagcacgag ctacagcgat agacacaccg aggattgcat tcgcacccaa
     2161 tttacctttg ttaggagtac cgtcaagtgc aatcatagca cggtcaatag cttgttgatc
     2221 acgtacatcg tagccaatga tagcttcagc aatgatgttg tttacgttgt caacagcttt
     2281 ttgtgtacca agaccaccgt aacgagattt gtcaccgtcg cgaagttcaa ctgcttcgtg
     2341 ttcaccagta gaagctcctg atggaaccat accacgtccg aaagcacctg attcagtgta
     2401 aacttctact tcaagtgttg ggttaccgcg tgagtctagg acttcgcgag cgtaaacatc
     2461 agtaataatt gacatttttt actctcctta tgagttaaat tttttacacc tctataatac
     2521 cttaaaaccc ctcctttttc aagaaaaaac gttatctttg tgcaactttt ccttaacttt
     2581 ataaagtaat cgctttcttt tgtctgtttt attctaactt ttatgatata ctgttttcat
     2641 gacagattta tcaaaacaat tacttgaaaa agctcatggt gggttaaaaa taaatccgga
     2701 tgagcaaaga cgctatcttg gtacttttga ggaaagagtt cttggatatg tagatattga
     2761 cacagcaaat agccctcagt tagaaaaagg ctttttattt attttagaaa accttcagga
     2821 aaaagcaaag ccactatttg tgaagatttc accaactatc gaatttgata agcaagtttt
     2881 ctacttaaaa gaagcaaaag aaactgatag tcaagccacc atagtatctg aagagcatat
     2941 tacttctcct tttggcctgg ttattcatag caatgcacca gttcaagtag aagaaaaaga
     3001 ccttcgactt gcttttccaa aactttggga agttaaaaag gaagaaccag ccaaaacatc
     3061 cttatggaag aaatggttta gctaaatctt gcacatattt aataagtgcc caatattggc
     3121 agccgtgcgc tccagataga gactggcatt tttcaaacta tcttctaaag gttcactttt
     3181 ctccaaaata gaaaagacag cttggatatt ttcaaatggt aggggaggta aatcttcagc
     3241 aagactaccg caaatagcaa taacaggaac tccaacaggg gttctttttg caacacctat
     3301 aggcgctttc ccagcaaagt tttgactatc aagtcttcct tctccaacaa caaccaagtc
     3361 agcatctgaa actttcttat caaagttgat taagtccaag caggtatcaa ttccagacac
     3421 gatacttgcc tgagcaaagg cacacaaacc agcagcaatc cccagtcctc catcattact
     3481 ggccgtgcca ccaacaccga tataaatatc tttaatccct ttagaaatga gatggagaat
     3541 caactctcca ataccacaag tttggatttg aagtggattt cgtttctcta gcggaatttt
     3601 tccaagacca accaagtcag ctacttcaaa tagtgccagt tccccttttt gaaaatagcg
     3661 catggcttct ttttgtccaa aagggtctgt cacttggatc catttttctt ttaggtcaag
     3721 agaatgtcgg atagcatcta cagtaccttc tcccccatca ccaacagggc agaggagaca
     3781 ttctacatct gctatcgatt gttggaagcc tctttttatt gcttcagcta cctgttgagc
     3841 tgtcaagctt tccttaaacg aatccggtgc aattacaatc ttcatatttt ccctcattct
     3901 aaacagtcaa tcaaaggaag aacttctaaa aaatccctct tgtcaacatg atgtggtatt
     3961 tcttttttga gcatttcttt ggaacaaaag gcgattccta actccgccga cttcaacatt
     4021 aatagattat taaccccatc accgattgcc accgttcttt ctttagaaag ttttagtttc
     4081 tttctccatt tttccagagt ttcttttttg acctggggac ttataatttg tccaactaat
     4141 tttcctgtta aaagaccttc tttgacttca agctggttgg cagtgaaata ggcaatacca
     4201 agggattttg ctaatctctc caactattgg tgtaaatcca ccagacacca gaccaactag
     4261 gatgccattc ttttggagaa tagagatgaa ctctgggaca tttagcgata gatgaattga
     4321 gttgaagacg ttatcaaaga ccaaaatagg aagaccttcc aacaaggaca ctctttttct
     4381 taaactgctt tcaaagacca actctcctcg cattgctcga cttgtaatct gcgaaatttc
     4441 cgcctcatga cctgcctctc tccctaaaag atcaatcact tcttctagga ttaaggttcc
     4501 atctacatcc aaaacacaca agccttttac ttgagacatc agttctcctc tctaaacagc
     4561 ctaaaaatcg tatgaagtca tcatacgatt ttatctatta attaactaaa ctatggtaca
     4621 agtcaaggta tgacttgcag gctgtatccc atgagaagtc actctccata gcttgttttt
     4681 gtaggtttct ccaaatgtct ggatggtttc tatacaagtc caatgctgtt tggaaagtcc
     4741 aatttaacca ataaggagat agattgtcaa agctaaagcc agtaccgctt ccttcgattg
     4801 gattgaaagc gcgaactgta tctcgcaagc ctccaacttc atggaccaat ggcaaggttc
     4861 cataacgcat agccatcatt tgagacaagc cacacggttc aaaacgactt ggcatgagga
     4921 agaggtcaca agcagcgtag atttcctgag caagtttgac atcaaaagtg atatttgctg
     4981 atagcttgtc tgggtaaatc tgagcaaacc atgagaaagc tccttcaaag gctggatcgc
     5041 cagttcccaa aagaacaatc tgaacatctt cttgcaagat atggtgaaga ctttcgacca
     5101 ccacatcaaa acctttttga cgtgtcaaac gagaaacaat tcccaccagt ggaacgtctg
     5161 ctctaacagg caagccaact ctttcttgca attttgcctt atttttggct ttcccagaca
     5221 aatcttcctg attgaaatga tagtctaaaa gagcatccgt ctgaggatta taaagatcag
     5281 catcaatccc attcacgata ccagatactt taccagactc cattttaaga atctgatcca
     5341 aattacatcc aaactgacta gtcataattt catgagcata gctaggtgaa acggttgaaa
     5401 cacggttcgc atagagaata cctgccttca tccagttcag acagttgttc catcgaaggg
     5461 tgccatcagc gtaacgttca aggccaactc caaacaaatc acccaacatt ccttctgaaa
     5521 attgtccttg gaattctaaa ttatgaatgg ttaaaactgt ttcaatgtcc tcataggctt
     5581 gaatccaaca gtatttttcc ttcaacaaga aaggaatcat agctgtatgg tagtcatgaa
     5641 catggagaag atcaggaata aagtcaatcc tttccatagc ctcaatggca gccagttgga
     5701 aaaaggcaaa gcgttctccg tcatcaaaat caccgtaaac atgaccacgg aagaaataat
     5761 attgattgtc aataaagtag aaggttacac catttaatac tgttttctta attccacaat
     5821 actgtctacg ccaaccaacg ctcacctcaa aatgaagcac atcttcaatc tgatttccaa
     5881 atttagcctc taccatatca tagtagggta aaatcactgc aacttcgtgc ccagctttta
     5941 ccagtgattt tggaagagcg ccaatgacgt ctcccaaacc acctgttttt gaaaagggtg
     6001 caccctctgc tgctacaaat aaaattttca tgaatgaata tcctctgtta ctttagcacc
     6061 tttcttaacc acaactggat gttctgcagt tcctcgaatc acaacaccaa gctcaacttc
     6121 aacccctttg tccaagatag catattcgac ctgagcccct tctccaataa caacacgagg
     6181 gaagagcagg ctatctttaa ccaagctatc cttatggaca tgaatattac gtgatagaac
     6241 agaattagct acttgacctt caataatact accagaggca aactgagaag tgcttacctt
     6301 agatgtatta gcataataag ttggctcttc gtttttgacc tttgtataaa tcttttggtt
     6361 tggtgagaaa agagaataga atttttgtga ttcaagcata tcgatatttg cttgataata
     6421 agatttaaca gagtgaatat tggctagata gcccgtgtac tcgtaggcaa aagctccctc
     6481 ttttacagcc aaatcccgta aaacatagcg caatttctct ggatgttctt ttttagcttc
     6541 ttcttccaag tgttcaatca accaaggtgt atcaacgaca aagatatctg tagacatatt
     6601 gaacgtttca gctgttgact tgctatcaaa gagtttatga gaaagaacat ggtctgtttc
     6661 atctacatcc aagattgcat ttacttctga aatatctttc ttagctagtt ttttataaac
     6721 tacagtgata ggctcttttg ttgtactatg taggtggaaa acttggttca aatcaatgtt
     6781 aataagaaca tcgcagttga gggcaaccgt ttggtttgag ccagaacgtt tcaaataagt
     6841 aagaagctgt tggtagtatt cttttccaac tgtactactt tctacacggg tattgtaaat
     6901 tcctagatag taatggctaa gaagggttga taagccccac tcgcgccctg aacgaatatg
     6961 gtcaaagact gagctgatat tatcctgctg gaaaatacca aagacactac gaacacctgc
     7021 attagcaagg cttgaaagtg ggaagtcaat caaacgatat ttcccaccaa atggcaaact
     7081 tgctactgga cggtggtccg tcaatgtcga catatcgtga aaaccaactg tatttcctaa
     7141 aatggcagaa tatttatcaa tcttcatctg ttgctacccc cactacttca ttatatccta
     7201 caacttgtac ttcatctgtt ccatcaattt cgacaccgtc agaaataatc gcaccttcac
     7261 caataatggc acgtttaatc ttagctcctt gaccaataat agctccactc atgataactg
     7321 aatcaaggac ttccgctcct tcgcgaactt gcgcgcctgt tgaaaggata gaatgtttaa
     7381 tagttccatc aacgaaacat ccgtctacaa ctaatgagtc ttccacatga gcatttgccc
     7441 cgaggaagtt tggtggtgaa atcaagtttc ttgagtaaat cttccattga cggttacgac
     7501 tatccaaggc attttctgga gaaatatact ccatgttcgc ttcccaaagt gactcaatag
     7561 taccaacatc tttccaataa ccactaaatt cgtaagcata aacactttca cctgactcaa
     7621 ggtaatttgg aatgacattt ttaccaaagt ctgacatgcc aaccttgctc ttttcagcag
     7681 cgactaacat attacgaagg cgttgccaat caaaaatgta gattcccata gaagcttttg
     7741 tagatttagg ttgagctggt ttttcttcaa attcaacaat acgattgtta gcatctgtgt
     7801 tcatgatacc aaaacggctc gcttctttaa gagggacgtc taaaactgct actgtcaagc
     7861 tggcattatt atccttatga gactggagca tatcatcata gtccattttg tagatgtggt
     7921 ccccagacaa aatcaagaca tactcaggat tgacactgtc gatatagtcg atattttggt
     7981 aaatagcgtg actagttccc tcaaaccaac gatttccttc acttgcagaa taaggttgaa
     8041 gaatagagac acctgaatta ataccgtcta gtccccagct tgaaccattc ccaatatggt
     8101 tgttgagagc aagtggttga tactgtgtaa ccaccccaac attatgaatc cctgagttgg
     8161 cacagtttga tagggcaaag tcaatgatac ggtagcgccc accaaattgc acagctggtt
     8221 ttgcgatgct ttgagtgagt ttaccgagac gagttccttg cccaccagca agaatcaaag
     8281 ctaacatttc atttttcatt ttctactcct ttttggtttt tatttgtgac ggttttagta
     8341 gatttcaagc gacgtttgat tttccataca cttgctccca tagccggtag ggtaaaggtt
     8401 aaggtctgct cataatcttt ccataggcct tcttgcgttt gaacagtttg attatgttct
     8461 ttccaaacgc ctccccactc ttccaactca gtattccata cttcttcgta aattcctgca
     8521 acgggtagtc cgattgtaaa atctttccgc tcaacaggta ccatgttaaa gatacagact
     8581 aacatttctc cctttttacc cttacgaata aaggaaagaa cactctggtc tcgattatcc
     8641 gcatcaatga tttcaatacc atcatagctg gtatcgattt cccacagaca gcgatgatct
     8701 ttgtaaaact ggtttagctg agaagtgaaa tacttcatct tagcattcat tgggtcttct
     8761 aggttagacc attccaactg ttcttcagat ttccattcta ggaattgacc gtattcgcta
     8821 cccatgaaga gcaatttctt accagggtga caaatttggt acgtatagag attgcgcaag
     8881 cctgcgaatt gattgtaacg atctccccac atcttatgca tcatactctt cttgccatga
     8941 accacttcat cgtgcgagaa tggcaagaga taattctcct tgaaaacata cataaagctg
     9001 aaagtcacca ggttaaagtc atatttacga taaatcggat cttcttcgta gaaacggagg
     9061 atatcattca tccagcccat gttccatttg tagtcaaatc ctagaccacc aatctctttc
     9121 attcccgtaa tcttggtcgc agacgaactt tcttctgcaa tcatcatcac atctggatat
     9181 tctaacttaa taacttcatt caagcgttga agaaaataat aaccttcata gttgagattt
     9241 ccgccatctt tattaggtgt ccatggagca ttatcatagt ccaaatagag catgttgcta
     9301 acagcatcca cacgaatacc atccaaatga tagacatcaa tccaatgctt aatgcaagaa
     9361 attaagaagg actggacttc attttttcca aggtcaaaat taagggcacc ccaaccatgg
     9421 ttatgagcct tattatggtc ttggtattca aaagtcggtg tcccatcata ataggctaag
     9481 gcatcatcgt tgatggtaaa gtgacctggt acccagtcca caataacccc aatattatgg
     9541 gtatgacact cctcgacaaa atcttgaaac tcctctggtc tgccataagc atgctctaaa
     9601 gcgaagtaac ccataagctg atacccccaa ctcaagccca aaggatggga catcaagggc
     9661 ataaactcaa tatgagtata gttcatttca acgagataag gaatgagttc atccttgagc
     9721 tgggcaaaac tataaggact gccatcagaa tttcttttcc atgatccagc gtgaacttca
     9781 taaatattga caggacgctc ttcaaagccc caacgttttc ttcgtgccag ccaaagtcca
     9841 tccttccatt tcttctcagg aagctctgtt aagattgccc ctgttcctgg acgagcctca
     9901 tacctgacag caaaagggtc aatcttcatc agttgatgac cattttgacg tgtgacatga
     9961 tatttgtaaa tatgcccttc ttgagccata ttggtaaaga cttcccagac cccaaaatca
    10021 tttcttacca ttggaatctg attttcaatc cagttggtaa aatcaccaac caagtgaaca
    10081 gcctgagcat taggtgccca aacacggaag gtatagccat gctctccatt tagttcttcc
    10141 ctatgtgctc ctagataatg ttggagataa aaattttcac ccgtcataaa ggtttttaat
    10201 gcttctctat tatccatata ctccccttct cctgtaagcg ttttctatgt ttttattata
    10261 ctaccttttt agagaagatt caagtaaatt actatacttc tttaattatt ttgaaaatct
    10321 acaacaagtt cacttactcg ttcaattgta aatcaatatt ttttcaaaaa attgcgaaaa
    10381 cgcctttctt tttctactat agtgaaatga aataaaacat gcgcaa
//
LOCUS       DAVKLX010000067        10039 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKLX010000067 DAVKLX010000000
VERSION     DAVKLX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.067"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..674
                     /locus_tag="VW325_001908"
     CDS             <1..674
                     /locus_tag="VW325_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEU9735241.1"
                     /translation="KRIKRLRRISGGDTYTIIYLKLLLLSLKDEGKLYYDGVESDFIK
                     ELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTEIPNLIGSETAWAEKKRRYR
                     QNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYK
                     NLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYD
                     VGESL"
     gene            1358..1519
                     /locus_tag="VW325_001909"
     CDS             1358..1519
                     /locus_tag="VW325_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735242.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1611..3290
                     /locus_tag="VW325_001910"
     CDS             1611..3290
                     /locus_tag="VW325_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9735243.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEERQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            3558..4124
                     /locus_tag="VW325_001911"
     CDS             3558..4124
                     /locus_tag="VW325_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735244.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            4446..4634
                     /locus_tag="VW325_001912"
     CDS             4446..4634
                     /locus_tag="VW325_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9735245.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            4696..5454
                     /locus_tag="VW325_001913"
     CDS             4696..5454
                     /locus_tag="VW325_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9735246.1"
                     /translation="MVVYIRQSKLLSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIDVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            5512..6366
                     /locus_tag="VW325_001914"
     CDS             5512..6366
                     /locus_tag="VW325_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9735247.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKDYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            6478..7362
                     /locus_tag="VW325_001915"
     CDS             6478..7362
                     /locus_tag="VW325_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9735248.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            7525..9264
                     /locus_tag="VW325_001916"
     CDS             7525..9264
                     /locus_tag="VW325_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEU9735249.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIWKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
     gene            9539..9994
                     /locus_tag="VW325_001917"
     CDS             9539..9994
                     /locus_tag="VW325_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735250.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSENLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
BASE COUNT         3622 a         1393 c         2098 g         2926 t
ORIGIN      
        1 acaaaaggat aaaaaggtta aggagaatat ctggaggaga tacctatacg ataatctacc
       61 taaaacttct actactaagc ctaaaagatg agggcaaact ctattatgac ggagtagaaa
      121 gtgattttat aaaagagtta gcactaacca tagatgaaac agacgatgat gtaatggtaa
      181 cagtaaatta tttaatcaat caaggcttat tagaagttgt aacagaaaat gatgaatact
      241 acctaactga aataccaaac ttaatcggat cagaaacagc ctgggcagag aaaaaaagaa
      301 gatacagaca aaataaacag aggacattgt ccttaatgag tccaacccat gtccgacaag
      361 agatagagat agagaaagat atagagatag ataaagagag agagggagag atagataaaa
      421 agtccacccc cattttttat ggagaattca aaaatgtaag gttaagcaaa gaagaatata
      481 aaaatctaaa agaaaaatta aactcacaca cagatataat gataaataaa ctatccagat
      541 acatggaaag cagtggtaag acctatcaaa accactatgt gacaatctta aaatggtatg
      601 aagaagataa agacaaacta agacagaaag gtttaaataa aaaaatgaat tatgatgtag
      661 gagaatcttt atagataaaa agaggtggaa aggcactatt aaagacttta agtttaaaaa
      721 ggtatcaaag tatgccagag ataaaaaaat ataaatatga cactttaaag gcgattagaa
      781 aattaaagaa atgctagaag acttttagat gacagttacc aagagggtct aattttcact
      841 gctacttatg cagtaggaat tggacctttt tatataagca aattctattt caaaattggt
      901 taccaagaaa gacttctttt cactgttaag tataagaggg taatttatag aattttgtta
      961 catcaagata aatattttct gatggtcaaa ttttaaaaag attaataact ttcgataaat
     1021 tttgaacctt aacaattgaa tagaccaaga caagacgtta attatttttg gagcgtaata
     1081 acctatccgt caagatcttt cggctgaata attcggcaaa aaaagttctg ctaagcaaaa
     1141 agaaagtgag cgataagtaa gagtttgaat atagggaggg ataccctatc cgctatgaag
     1201 aaaatgagga taatgatact tctaaggttg aagccggctc atccggaata gaggcggagg
     1261 tgagattcct atgttgctaa tccaaagcac ttgtcaatgt caaaaaactt aattaaacaa
     1321 ataattatta gatataaaac tttagaggag gtatatattg gacaatgact ggaatggatt
     1381 ggctgattta atagcaaatc tgattgcaaa atatgctggt gctttagatt tagataatct
     1441 acccgatcca acgccagcaa aaaatcaaga gatgaaaaat agttttgaca tggcaaaaac
     1501 acaaattgag acggactaat aaaagtgtta taatatactt gaaatgatag tccaaactta
     1561 aattaaaaaa attatatatg atatagaaaa attatattga ggttaatact atgtcaaaag
     1621 aaaaaataaa agtatacctc tatacacgag tatctacgtc aatacagata gaggggtatt
     1681 cgttagaggc acaaaaatca cgaatgaaag cttttgctat ttacaacgat tatgaaattg
     1741 ttggagagta tgaagatgcc gggaagtctg gcaagtctat tgaaggtaga atacagttca
     1801 atcgaatgat ggaagatata aaatctggaa aagatggagt atcttttgtt cttgtgttta
     1861 agttatcaag atttgcaaga aatgccgctg atgttctttc tacacttcag ataatgcagg
     1921 attatggagt taatcttatc tgcgttgaag atggcattga ttcatctaaa gatgctggga
     1981 aactaatgat ttctgtttta tcagctgtgg ctgaaataga aagagaaaac atccgtattc
     2041 aaacaatgga aggttgcatt caaaaagcaa gggaaggaaa atggaatggt ggctttgctc
     2101 cgtatggata taaacttgaa gacggcaagc tgtttataaa tgaggaagag gcagttgcaa
     2161 taagaacgat tttcgaccag tatgtaaata ctacgatagg agccaatggg atatctaaat
     2221 acttagagaa tcatggaatt agaaaaatcc caagacagaa tggtaagaat cctttgtttg
     2281 atgcaggtct tataagaaag atattaaaga atcctgtata taatgggaaa atagcatttg
     2341 gaagaagaac tttagaaaaa gttcatggta caagaaatga atataagcag gttgaacaag
     2401 atgaatatct aatatctgaa gggatacatg aagctatagt ttccgatgaa gtttggcaag
     2461 ctgctcaggt taagctaaaa tctcaagcaa agaaatatga gcatgtgaat aaaggaaaag
     2521 atacacgcac acacttgctt tctggaattg taaaatgccc gatatgtgga gtgggaatgt
     2581 ttgggaacaa gtgtatcaag aaaaagaaag atggcacaaa gtataaagat ttttattact
     2641 atggttgtaa acataggcag atgataagag gtcataagtg tacattcagt aagcaaatta
     2701 gagaagaatt gttagatgat gctgttgcag aggtgattgt caagatagta agtaatccga
     2761 aatttgcttc tatgatgcaa gaaaaaatca acatgaaggt ggatacctct gaaatagaaa
     2821 aagaaataga taattaccaa aaggaattga gaaagagtca ttctacaaag tttaagctaa
     2881 ttgaggaaat agataattta gatgttgaag ataagcatta taagagaagg aaacaggatt
     2941 tagatgatag actatatcgt atgtatgaca aaatagatga attagaatca tcactaattg
     3001 atgcgaaagc aaagaaacag actattgaag ctgaaaagct tacaggagat aacatatata
     3061 aggttctaat ctattttgat aagctctata aagtcatgaa tgatgtagag cgtaggcagt
     3121 taatttcagc tttgatttct gaaattcaag tttatgaaga aagacaatct aacggacaat
     3181 ggctaaaatc cattactttt aagctaccaa ttatagaaga aaatctcaac ataggtttgg
     3241 acaatgatga acaagttgaa tgtgtaagtt tattagagaa acgtagttaa tcctcaaaag
     3301 gttccccgaa ccttttgagt cccactgacg catcacactg aggcggtatc actgcttgta
     3361 cgagttgaag catcagcaaa gtaaaagtag atgttcagcc aatgcgacga ggtgagaccg
     3421 aaggtcgata aggtgtgttc tgggtataag actcgtaaag taagtaggaa atatgatgag
     3481 atgatacaga gagcctgaat tggtcaggtg acgtgcttat ttacattagc ctgataatga
     3541 aaatggagag attatcgatg gttatattgg aattgtacca aaatgattat tctaaagatt
     3601 tagtagcatt tgattccata gaagatggga aagcctttgt agctcaaatc ccgggataca
     3661 ctctggaaac agaagatggt tttgaagtgg agtattttaa tcctaaaaac ataccggatt
     3721 atatggaaat tatttttaac ggaaatattg ttcctttatc taaatttatg ttcgatcctg
     3781 aggaaaatgt aaacattatt tggaaagaga tttcaaattt atccctcaaa aatgatagag
     3841 tgatcgaagg atattcaaaa attgatgcat atgtcgttaa caaccatgaa gttaaagcct
     3901 atgtcgaaac aagagaaacg aactatcgta aagcaaaaga cttcttagaa agccgcggat
     3961 atgaaatcga cagaagcttt ttcggaagtg aagacggtga agcgattctt tacagaaaaa
     4021 aagggatcga agtttggcat ttcttgtgtc acttagaccc aatgtttgta gagattgaag
     4081 atgtagaagg atatgttaaa gaagaagttg gagagattca atagtcaacc aaagttcctt
     4141 gtgagtatat acctttgcta gtaaaagagt caaaacgtta taacgaaggg ggggaagaaa
     4201 acccttgagt gtaagtcttt ttcttgttta gagggaaagt attagagtag ccgctcataa
     4261 tttatactag atttgataac tgatttgaga cagattttat ttctataaat attgtgaaaa
     4321 ttttttaata ccaaagattt aaagttactg tgttaaaaat cctctccact tttaaactaa
     4381 tgtattataa aagccagttg acacaagtgt tacacctgtg atagaattaa cttgaggtga
     4441 tgaaaatggc aaccgataaa aatagaataa tgattagctt ggatgataaa aatctagaga
     4501 aacttgaaaa tttagtagaa gatgctagag atagaagggg aatgcgacta acaaagtccc
     4561 aggttattga attactccta aataccgttg attatttcga tgatattatg ggggctattt
     4621 actcaaaaaa ataagagcaa aattgctatt attttttaca aattggtgta acactagtga
     4681 tataggagtg atgaaatggt tgtttatatt agacaaagta aattactaag tgaggtttca
     4741 attaataaat acaatgctca agtaggtgcc tacttacaag gagaagaagt tattttatat
     4801 caatcttttt ctgaaataaa agagttaaca agtgaagata tagatgtaga ttatataatg
     4861 gagactagag cattactaaa aatgatgggc ttaaacgttc cggtttatga ctatcctatt
     4921 gagcttaaag agttttatgg tcgaaagatt tatgctggta ttttaggaga gattgtgaat
     4981 atacctgata attggggaaa atttattaag cctaaagctg gttcaaaagt ctttactgga
     5041 agagttgtta atggaaccca tgatttaata ggtattggtc tacctttcga ctatcctata
     5101 tggattagtg aggttgtaga attcatagct gaatggcgtt gttttgtgtt agatggtcgc
     5161 gtattagatg ttcgacccta tacaggtgat tatcatgcac aatttgatgc aagtgtaatt
     5221 gatgaagcga tatcatgttg gaaagatgcg ccaatagctt atggactaga tatcggtgtt
     5281 actcgcgatg gcagaacact tgttgttgaa gtaaatgatg gttatgcatt gggaaattat
     5341 ggcctatctc ctttaaaatc aatcaatttt catagagcta ggtggaaaga aatggtaaaa
     5401 ccctattttg aaaaaaatga aatttttaaa atccaacagg atgttatttt ctaaaataaa
     5461 tatggtagat aaccaatata aaaagcatca aaatgaacaa ggaggaatct gatgcttgga
     5521 gcaattattg gagatattgt cggttctgtt tacgaatgga acaatatcaa aacaaaggac
     5581 tttcctttat ttcggaagga ctgctttttt acagatgaca cggttatgac ctgtgctgtt
     5641 gcagaagcaa ttatgaatgg tggacaaaaa gacgacttca ttgacgcgat gaagaaatat
     5701 ggcagaatgt atccgaatgc tgattacggt gctcggttta atgcgtggct aaacagcgat
     5761 aaccgtgagc cttataatag ctttgggaat ggatcagcta tgcgtatttc tccatgtgct
     5821 tggatcatgg actgtggttt ttatgcgaaa actggtatat ggccatcatc tagaggactt
     5881 acgagtcttt ctgcagaggt aactcataat catccagaag gtgtcaaagg cgctatggct
     5941 acagctgatg ctatctttct atgtcgtttt tactttggtg gttattgtag agagtacgag
     6001 caatcaatta acgacaatcc tacagagtgt aaaagacgaa ttaaggatta tatagaaaaa
     6061 gaatacgatt acaatctatc tcaaacttta gatgaaatcc gtcctaacta tcgttttaac
     6121 gaaacatgtc aggaaactgt ccctcaagcc attatcgcct ttcttgaaag tagagacttc
     6181 gaagatgcga taagaaatgc catctcactt ggtggcgaca gtgatactct cgcagcaatc
     6241 acttgtagca tagcagaggc agcttacggt attcctgatt ggatcaagga taaggactat
     6301 tcttacttgg atgaaccctt aaaggatgta gttaggcgat gggaaaatag aataaaagcg
     6361 tattaatctc cagtacgtca gatttctctt ttctgtattt tcattgtata ataggattac
     6421 atttacttgc atacgacgaa atgtaaacat ttctgtttaa aattaaggat aaaatcaatg
     6481 aaaaaagcaa tggtaattat caaccctact tctggtggcg agaaggcttt ggattacaaa
     6541 gaaaagctgg agaataaagc aaaagaatac tttgaatatg ttgaaaccaa aattaccgaa
     6601 aaagcgctgg atgcaacaca ttttgctgaa gaagcttctc gtgagcagta tgatgcagtg
     6661 gttgtgtttg gtggagatgg aactgtcaat gaagtcattt caggtattga tgagagagac
     6721 tacattccta agttagggat tatcccaggc ggtacgggta acctcattac aaaacttttg
     6781 gaaatcaatc aagacatcga tggcgcaatt gacgaactgg attttgattt aaccaataag
     6841 attgatatcg gtaaagcaaa tgacaactat tttggttata tctttagtat cggttctctg
     6901 cctgaggcga ttcacaatgt tgaaatagag gacaaaacaa aattcggtat tttaacctat
     6961 gctgtaaata ccatgaagtc tgtcatgaca gatcaggtct ttaacattaa ggttgagaca
     7021 gaaaatggaa attatgttgg tgaagctagc catgttttgg tccttttgac aaattacttc
     7081 gctgataaga aaatctttga agaaaacaag gacggctatg ccaacatttt gattctgaaa
     7141 gatgcctcta tattctccaa attatccgtc attcctgatt tattaaaagg ggatgttgtc
     7201 gcaaatgata atatcgagta tatcaaagcg cgtaatatta aaatctcttc agatagtgaa
     7261 ttggagtcag atgttgacgg agataaatca gataacctac ctgtagaaat caaagtccta
     7321 gctcagcgag tagaagtatt ttcaaaaccg aaagaggatt agtatataga gaaagccttt
     7381 tttaaggctt tttgtatact ttaaaagata gttcctttaa caacggacat tccttgcaaa
     7441 tagtttacaa aaatagtata ctggattcat tgagcttgaa aacgtttgcg taaaatttga
     7501 atgaatactt taggagacaa attgatggaa ttgagtgcta tttaccatag gcctgagtcg
     7561 gagtatgcct atctttataa ggataagaaa ctccatattc gaattcgaac taagaaaggg
     7621 gacattgaaa gcatcaactt gcactatggg gaccctttta tctttatgga ggagttttat
     7681 caggatacaa aagaaatggt caagataact tctggtacct tatttgacca ttggcaggtt
     7741 gaagtgtcag ttgactttgc acgtatccag tatctctttg agctcagaga tacagaaggt
     7801 caaaatattt tgtatggcga taaagggtgt gtggaaaatt ctctagaaaa tcttcatgca
     7861 attgggaatg gatttaagtt gccttatctt catgagattg atgcctgcaa ggttcctgac
     7921 tgggtttcaa atacggtatg gtatcagata tttcctgaaa gatttgccaa tggcaatgct
     7981 ctattaaacc cagaagggac tttagactgg gattcatctg tcacacctaa gagcgatgat
     8041 ttctttggtg gtgatttaca ggggattatt gatcatatgg attacttgca agacttgggt
     8101 attactggac tatatctttg tcccatcttt gaatctacaa gcaatcacaa gtacaatacg
     8161 acagattact ttgaaattga ccgtcatttt ggagacaagg agacctttcg ggaactggtg
     8221 gatcaagcgc atcatcgtgg catgaaagtc atgctggatg cggtatttaa tcatattggt
     8281 tcgcaatctc ttcaatggaa aaatgtcgtc aaaaatggtg aacagtctgc ttataaggat
     8341 tggttccata ttcaacaatt cccagtgaca actgaaaagc tagttaataa gagagactta
     8401 ccctatcatg tttttggttt cgaggactat atgcctaagc taaatacagc caatccagag
     8461 gtcaagaatt atcttttaaa ggttgcgact tattggattg aagagtttaa tatcgatgct
     8521 tggcgtttgg atgtggctaa tgagattgac catcagttct ggaaggattt tcgtaaggca
     8581 gttttagcta aaaatcctga tctttatatc ctaggagaag tctggcatac atctcagcct
     8641 tggctaaatg gagatgagtt ccatgccgtc atgaattatc ctttatctga tagtatcaag
     8701 gactatttct tacgaggaat taagaagaca gaccagttca tcgatgaaat caatggagag
     8761 tctatgtatt acaagcagca gatttcagag gtcatgttta atctcttgga ttcacatgat
     8821 acagagcgaa tcctgtggac ggccaatgaa gatgttcaac tggttaaatc agccttagcc
     8881 tttctctttt tacaaaaagg aacaccgtgc atttattacg gaaccgagct agccttgact
     8941 ggaggaccag atccagattg tcgtcgttgt atgccttggg aacgtgtatc aagtgacaat
     9001 gatatgctga actttatgaa gaggctgatt aagatttgga aatacgcgtc agtaatcatt
     9061 tcgcatggca agtatagcct tcaagaaatc aaatctgatc tagtagctct ggaatggaaa
     9121 tacgaaggac ggatcctcaa agcaatattc aaccaatcaa cagaagatta tcttttagag
     9181 aaagaagcag tagcactagc aagcaattgc caagaattgg agaatcagct tgtcatctct
     9241 ccagatggat ttgtgatttt ctaaaaacta gttgatgaag attatggtac atttcatatc
     9301 ttatatagta taataaggct agttactaaa cttgtaaagg agaacttaaa tgaattgtag
     9361 aggacatgaa acaagacaaa gaattgttag agattttgaa gtttagccta aagcacatat
     9421 taagctgtta gcaaatcaac aaaaacatag tgatgcagga gcaactattg aagatgaata
     9481 ttatgtattt atcgctgaga gtaaaattga tggcaagaag gaagttattc agtgttgcat
     9541 gggtgcggca agggattttt tagaactaat taatcacaaa gggctacctc tttttaatcc
     9601 gcttgtaggt gattctcatg taaataatag acaagaatat gacaatacag ggagtgaaaa
     9661 tttacaacct gaaaagtgga atgaaactgc aaagcagctt tataatgcta taatgtggtt
     9721 gattatttta tggaatgcta agccggatac acctttattt aattttaaag acgaagtaat
     9781 taagtataaa acatatgagc cttttgaaag cagtataaaa agagtaaata ctactataaa
     9841 gaatggtagt aaagggaaaa ctctgactga gatgattaat ggctacagag cggataacga
     9901 tattagagat gaaatttgta actttaatat tctgaaaaat aaaattcgtg atatgaaaga
     9961 ccaacaagga aatacaatgg aatcttactt ttagttattg ttgaattttg ggtattctat
    10021 aaaatatcct aattgagat
//
LOCUS       DAVKLX010000068         8943 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKLX010000068 DAVKLX010000000
VERSION     DAVKLX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8943
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.068"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(1..679)
                     /locus_tag="VW325_001918"
                     /pseudo
     CDS             complement(1..679)
                     /locus_tag="VW325_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            935..1780
                     /locus_tag="VW325_001919"
     CDS             935..1780
                     /locus_tag="VW325_001919"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9735251.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(1927..2988)
                     /gene="dinB"
                     /locus_tag="VW325_001920"
     CDS             complement(1927..2988)
                     /gene="dinB"
                     /locus_tag="VW325_001920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9735252.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            3381..5705
                     /gene="pflB"
                     /locus_tag="VW325_001921"
     CDS             3381..5705
                     /gene="pflB"
                     /locus_tag="VW325_001921"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9735253.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIDPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDKYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(5763..7025)
                     /locus_tag="VW325_001922"
     CDS             complement(5763..7025)
                     /locus_tag="VW325_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9735254.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGGFPSLIHLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTVYRKLKQFHFQEDYTTLPEILSWDE
                     FSYQKGKLAFIAQDFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDMSGSY
                     IPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWKFILK
                     DSRKLSLKPFYARTFRETLTPRECLKKIFTLVPELKDYYDLYQLLLFHLQEKNTDQFW
                     GLIQDTLPHLNRTFKTTLSTFICYKNYITNAIELPYSNAKLEATNKLIKDIKRNAFGF
                     RNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(7290..8819)
                     /gene="rlrA"
                     /locus_tag="VW325_001923"
     CDS             complement(7290..8819)
                     /gene="rlrA"
                     /locus_tag="VW325_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
                     /protein_id="HEU9735255.1"
                     /translation="MLNKYIEKRITDKITILNILLDIRSIELDELSTLTSLQSKSLLS
                     ILQELQETFEEELTFNLDTQQVQLIEHHSHQTNYYFHQLYNQSTILKILRFFLLQGNQ
                     SFNEFTQKEYISIATGYRVRQKCGLLLRSVGLDLVKNQVVGPEYRIRFLIALLQFHFG
                     IEIYDLNDGSMDWVTHMIVQSNSQLSHELLEITPDEYVHFSILVALTWKRREFPLEFP
                     ESKEFEKLKNLFMYPILMEHCQTYLEPHANMTFTQEELDYIFLVYCSANSSFSKDKWN
                     QEKKTHTIQLILQHTRGKHLLSKFKNILGNDISNSLSFLTALTFLTRTFLFGLQNLVP
                     YYNYYEHYGIESDKPLYHISKAIVQEWMTEQKIEGVIDQHRLYLFSLYLTETIFSSLP
                     AIPIFIILNNQADVNLIKSIILRNFTDKVASVTGYNILISPPPSEEHLTEPLIIITTK
                     EYLPYVKKQYPKGKHHFLTIALDLHVSQQRLIYQTIVDIRKEAFDKRVAMIAKKAHYL
                     L"
BASE COUNT         2658 a         1643 c         1813 g         2829 t
ORIGIN      
        1 atgatttcga attcacgagc aggagatgtt gttccgatag aacgaggtgc gactgagagg
       61 atatcctgaa ttttagaagc ggtcaatcgc ttaatttcta tcagcttatc aaatcctgcc
      121 tcaagccttt tctgaggatt agggtagcat gtcaagagtt ggtaggtata ttctgaatgc
      181 tttccaacga ttttatccaa ctcaggaaag atgatatcaa gacaacgagt gtattgcact
      241 ttccaatcag actgtttttt cttgagacga tgaatatgtc tagccagtat ttttagttct
      301 acttgccgat tatcgtgttg aaattgttca cgattggggt cagaaagaag tttaagagcg
      361 atgccatgag cgtctttctt atccgtttta gtcttgcgaa gtaataatga tttggcaaat
      421 ttcttgatga gcaaaggatt gtaggtgtaa actttatatc cttgttcatg caggaagttc
      481 agtagattaa aggcataatg tccggtatct tcaagagcga tgagatagtc ttggtcgagc
      541 tgtcgaagag acagatctaa aagttcaaaa ccagctttat tatttgaaaa agtgagtggt
      601 ttaagaacag ttttcctcga acattcaagg ctgtaacatc gtgtttattt ttagcgacat
      661 caatgcccac ataaagcatg ggagtacctc cagatatagt atttcaagtc tacttggtta
      721 tccacgaact ttttgccttg ttaccttaga cgagataaaa cgtctatgcg ttatcaaact
      781 cattaccaat tgaaacaaaa agctgtggtt agaccctttc ggaaatcgtc aagcgattgg
      841 aggaaatgaa ctaatccaca gtggcttatt ccaagtatac cacttgggct ttggcagtag
      901 ctaactgcgt taaatataat ataaggagaa atatatgtat cttattgaaa ttttaaaatc
      961 tatcttcttc gggattgttg aaggaattac ggaatggttg ccgatttcca gtacaggtca
     1021 cttgatttta gcagaggagt ttatccaata ccaaaatcaa aatgaagcct ttatgtccat
     1081 gtttaatgtc gtgattcagc ttggtgctat tttagcagtt atggtgattt attttaacaa
     1141 gctcaatcct tttaaaccga ctaaggacaa acaggaagtt cgtaagactt ggagactatg
     1201 gttgaaggtc ttgattgcca ctttgccttt acttggtgtc tttaaatttg atgattggtt
     1261 tgatacccac ttccataaca tggtttcagt tgctctcatg ttgattatct acggggttgc
     1321 cttcatctat ttggaaaagc gcaataaagc gcgtgctatc gagccaagtg taacagagtt
     1381 ggacaagctt ccttatacga ccgctttcta tatcggactc ttccaagttc ttgctctttt
     1441 accagggact agccgttcag gtgcaacgat tgtcggtggt ttgttaaatg gaaccagtcg
     1501 ttcagttgtg acagaattta ccttctatct tgggattccc gttatgtttg gagctagtgc
     1561 cttaaagatt ttcaaatttg tgaaagccgg agaactcttg agctttgggc aattgttttt
     1621 gctcttggtc gcgatgggag tagcttttgc ggtcagcatg gtggctattc gcttcttgac
     1681 cagctatgtg aaaaaacacg acttcaccct ttttggtaaa taccgtatcg tgcttggtag
     1741 tgttttgcta ctttacagtt ttgtccgttt atttgtataa gaaaaacctt gaaggggcaa
     1801 ctcttcaagg ttttatactc ttagaaaatc tcttcaaacc gcgtcagctt tatctgcaac
     1861 ctcaaaacag tgttttgagc agcctacggc tagcctccta gtttgctctt tgattttcat
     1921 tgagctttaa aatccagtca tggtaatccc caataggcgg acacctcttt ctttctcgct
     1981 taattcttca tagagttgca gggctatttg gcttatctga ctagcatctt gtgttttttg
     2041 agcaagactt tttcgtttgg taagagttga aaagtcctcg tagcggattt tcaaaatgac
     2101 aatttttcca gctttttctt gttgatgtag attgagagcg actttttctg atagaagagt
     2161 cagctctttt ttgatatctt cctcggcacg gagaatcttc ccgtaggttt tctccttgcc
     2221 gattgattta cggatgcgat tggatttgac tggagagttg tgaatgccac gagcctttcg
     2281 atatagatca tagcctagtc taccaaaacg gtctattagg gtcacctcag gaacttcaag
     2341 taaatcagca ccagtaaaaa cgcccatttg atgaagacgt tctactgtct tttttcctac
     2401 tccatgaaat ttggaaatat ccatttgttt gagaaaatcc tcagcctgtt caggtagaat
     2461 cactgtcaaa ccatgaggtt tttgataatc actcgccatt ttagctaaga acttgttgta
     2521 ggaaataccg gcagaagcag ttagatggag ttcttgccag atatcttttt gaatgaggcg
     2581 agcaattttg accgctgact tgataccaag tttattttct gtcacatcca aataggcttc
     2641 gtcaatgctc atgggttcaa tcaaatctgt atagcgctta aaaatagctc gaatctggag
     2701 tcccacagct ttgtatttct cataatttcc tgagataaag acggcttggg gacaacgttc
     2761 ataagcttcc ttggaactca tggcagaatg gacaccaaaa gctcttgcct cataactaca
     2821 ggtagaaacg actccccgtc cacctgtttg ccgagggtcg cttccaataa tgacaggttt
     2881 tcctctgagt ttaggattat ccctgatttc cactgcagca aaaaaggcat ccatgtcaat
     2941 atggatgatt tttcttgaca aatcatttaa caaaggaaaa atcaacatgc ctagcacctt
     3001 tttatactct tcgaaaatct cttcaaaccc cgtcagctct atctgcaacc tcaaaacagt
     3061 gttttgagct gacttcgtca gttctattta caacctcaaa gcagtgcttt gagcagcctg
     3121 cggctagctt cctagtttgc ttttcgattt ccattgagtg ttactgctta ttttctttta
     3181 ttataccctt ttttctgaaa aaaagaaaaa aggactttat tttttcaaaa atataataca
     3241 gtttgaaata aaatatagac tgttttagaa aagaaagttt aaaaataggg atttttcact
     3301 tgttgaaatc ggttactata tggtatactt gtctcatgaa tgtaacagat gactgttact
     3361 agaaaaaaga ggacattaat atggttgtta agacagttgt tgaagcacaa gatatttttg
     3421 acaaagcttg ggaaggcttc aaaggcgtag attggaaaga aaaagcaagt gtatcacgct
     3481 ttgtacaagc taactacaca ccttatgatg gagacgaaag cttccttgca ggaccaacag
     3541 agcgttcact tcacatcaag aaaattgtag aagaaactaa agcacactac gaagaaactc
     3601 gtttcccaat ggacactcgt ccaacatcta tcgctgatat ccctgctgga tttatcgaca
     3661 aagaaaatga agttatcttt ggtatccaaa atgatgaact cttcaaattg aacttcatgc
     3721 caaaaggtgg tatccgtatg gctgaaacta ctttaaaaga aaatggatac gaaccagacc
     3781 cagctgttca cgaaatcttc actaaatatg taacaacagt taacgacggt attttccgtg
     3841 cctacacttc aaatattcgt cgcgctcgtc acgcacacac tgtaactggt cttccagatg
     3901 catactcacg cggacgtatc atcggtgttt acgcacgtct tgctctttac ggtgcagact
     3961 acttgatgca agaaaaagta aatgactgga atgcaatcaa agaaatcgat gaagaaacaa
     4021 tccgtcttcg tgaagaagta aaccttcaat accaagcatt gcaacaagtt gttcgcctgg
     4081 gtgaccttta cggggttgat gttcgcaaac cagcgatgaa cgtgaaagaa gcaatccaat
     4141 gggttaacat tgctttcatg gctgtctgcc gtgtgattaa cggtgctgct acatctctag
     4201 gtcgtgtacc aatcgtattg gacatctttg cagaacgtga ccttgctcgt ggtacattta
     4261 ctgaatcaga aatccaagaa ttcgttgatg atttcgttat gaaacttcgt acagttaaat
     4321 ttgctcgtac aaaagcttat gaccaattgt actcaggtga cccaaccttt atcacaactt
     4381 ctatggctgg tatgggtaac gacggtcgtc accgtgttac taagatggac taccgtttct
     4441 tgaacactct tgacaacatc ggtaactctc cagagccaaa cttgacagtt ctttggactg
     4501 acaaattgcc atacaacttc cgtcgctact gtatgcacat gagccacaaa cactcttcta
     4561 tccaatacga aggtgtaaca acaatggcta aagacggata tggtgaaatg agctgtatct
     4621 catgctgtgt gtctccactt gatccagaaa atgaagaaca acgccacaac atccagtact
     4681 tcggtgctcg tgtaaacgtt cttaaagccc ttcttactgg tttgaatggt ggttacgacg
     4741 atgttcacaa agactacaaa gtatttgaca tcgatcctat tcgtgacgaa gttcttgaat
     4801 tcgaatcagt taaagcgaac tttgaaaaat ctcttgactg gttgactgac acttacgtag
     4861 atgccttgaa catcatccac tacatgactg ataagtacaa ctacgaagct gttcaaatgg
     4921 ccttcttgcc aactaaacaa cgtgccaaca tgggattcgg tatttgtgga tttgctaaca
     4981 ctgttgatac attgtcagcg attaaatacg ctacagttaa accaatccgt gatgaagatg
     5041 gctacatcta cgattacgaa acaatcggtg actacccacg ctggggtgaa gatgacccac
     5101 gttcaaacga attggcagaa tggttgatcg aagcttacac aactcgtcta cgtagccaca
     5161 aactatacaa agacgcagaa gctacagtat cacttttgac aatcacatct aacgttgctt
     5221 actctaaaca aactggtaac tcaccagttc acaaaggtgt atacctcaac gaagatggtt
     5281 ctgtgaactt gtctaaactt gaattcttct caccaggtgc taacccatct aacaaagcta
     5341 aaggtggttg gttgcaaaac ttgaactcac tttctagcct tgactttagt tatgcagctg
     5401 acggtatctc attgactaca caagtatcac ctcgcgctct tggtaagact cgtgatgaac
     5461 aagttgataa cttggtaaca atccttgatg gttacttcga aaacggtgga caacacgtta
     5521 acttgaacgt tatggacttg aacgatgttt acgaaaaaat catgtcaggc gaagacgtta
     5581 tcgtacgtat ctctggatac tgtgtaaaca ctaaatacct cactccagaa caaaaaactg
     5641 aattgacaca acgtgtcttc cacgaagttc tttcaatgga tgacgccttg gatgcattga
     5701 gctaatcaag ttcttgaata ataaaaaggg ctctttgtca actgtagtgg gttgaagaaa
     5761 agctaagctc gagaaaggac aaattttgtc ctttcttttt tgatgttcag agcgatgaaa
     5821 atccgttttt tgaagttttc aaagttccga aaaccaaagg cattgcgctt gatgtctttg
     5881 atgagtttgt tagtggcctc aagtttagcg ttagaataag gcaattcaat ggcgttagtg
     5941 atgtagtttt tatagcaaat aaatgtgctc aaagtggttt taaaggtgcg gttgagatga
     6001 ggtaacgtgt cttgaattaa gccccaaaac tggtcagtat tcttctcttg tagatgaaat
     6061 aggagtagtt gatacaggtc atagtaatct ttaagttcag gtactagagt aaagattttc
     6121 ttcagacact ccctaggagt taaggtctct ctgaaagttc tagcatagaa aggcttaaga
     6181 gagagtttcc gactatcttt taggataaat ttccagtaat atttaagagc tctgtattcc
     6241 agagatttat catcaaattg cttcatgatg ttgattctag tctgattaag agccctgctc
     6301 atgtgttgga caatgtggaa acgatcgaga acaattttag cattgggaaa taatttctta
     6361 atgagaggga tataacttcc agacatatca acagtgacga ctttaacttt ttttctagct
     6421 tctttcgagt acttgaagaa atgatttcgg atggttgttt gacgtctgtt atcaagaatg
     6481 gtcatgattt tcttagtgtt gaaatcctga gcaatgaaag ccaatttccc cttctggtag
     6541 gagaattcat cccaggagag gatttcaggc aaagtggtgt aatcctcttg gaaatgaaat
     6601 tgcttgagct tacgatagac ggtagaggta gaggtagaga tggctaattt agaagcgata
     6661 tgtgtaagag cctctctgtt gagtaggagt tgggcaattt tctgtctcac catttccgag
     6721 atttggcaat ttttctgaac gagagttgtt tcagctacag tgactttccg acaggacttg
     6781 cattgaaatc gtctcttttt caaatgaatg aggctaggga aaccaccaat ctcgataaaa
     6841 gggattttag aaggcttttg gaagtcgtat ttgatttgtt ttcctttaca gtgtttacat
     6901 ttaggtgggt gataatcaag tgtagcgaag acttcgatat gggtatcgtg ctgaatggct
     6961 ttatttaagg tgatgttttt gtcttttatt ccgatgagta atgtggtatg attgatgtgt
     7021 tccataagat actttctaat gagttgttta ggcgcttttc attataagtc ttatgggact
     7081 tttttgatac tcaaaaagcc ctataatctc cacagtggga tttacccact acagaaatta
     7141 tagagccaga aaaaacactt ttgttcacta gcagaaacta gagagcagaa gtgtttttct
     7201 gttcagattt acccaaaact gggaaatatg gggataagaa tagagatggc ttaggaagcc
     7261 cctttttgtg tgtagacagt acgatgaact tataacaaat agtgagcctt tttagcaatc
     7321 attgcgaccc gtttgtcaaa agcctctttt cggatatcta caattgtctg atagatgaga
     7381 cgctgttggc taacatgcaa atctaaggca atcgtcaaaa agtgatgttt ccctttggga
     7441 tactgctttt taacgtaagg caggtattct ttcgttgtaa taataatcaa tggctctgtc
     7501 aaatgctcct ctgaaggagg aggactaatt agaatattgt atcctgtaac agaggcaact
     7561 ttgtcagtaa aattccgtaa aataatggac tttattaagt ttacatctgc ttgattattt
     7621 aaaatgataa aaatcgggat agcaggtagt gaggaaaaga tggtttctgt caagtagagt
     7681 gagaaaaggt acagccgatg ctggtcgata actccttcaa tcttctgctc agtcatccac
     7741 tcttgaacaa ttgctttcga aatatgatac agtggcttgt cgctttcaat cccataatgt
     7801 tcgtaataat tataataggg aactagattt tgtaaaccaa acaaaaacgt tcttgttaag
     7861 aaagtcagtg ctgttaaaaa agaaagagaa ttcgaaatgt catttcctaa gatattcttg
     7921 aacttggata gtagatgctt tcctcttgta tgctgaagaa tcagttgaat agtatgagtc
     7981 tttttttctt gattccattt gtccttggaa aacgaagaat tagcagaaca ataaaccaaa
     8041 aagatataat ccagttcttc ctgagtaaaa gtcatgttgg catgtggctc taagtaagtt
     8101 tggcaatgtt ccatcaaaat cggatacata aagaggtttt ttaatttttc aaactctttg
     8161 gactcaggga actcaagtgg aaattcccga cgtttccaag tgagtgccac tagtatgcta
     8221 aaatgaacat actcgtcagg tgtgatttct aacagttcat gactgagttg agaattagac
     8281 tgcacaatca tatgtgtgac ccaatccata cttccatcat tcaaatcata aatctcaata
     8341 ccaaaatgaa actggaggag tgcaattaaa aaacgaatgc gatattcagg accaactact
     8401 tgatttttca caaggtccaa acctactgaa cgtagtaaca agccacactt ttgtcgtacg
     8461 cggtagcctg ttgcgatgga aatatactct ttttgtgtaa attcgttaaa gctttgatta
     8521 ccttgtagta gaaagaagcg gagtattttt aaaatagttg attggttata aagctgatgg
     8581 aagtaataat tcgtttgatg agaatggtgt tcgattaatt gaacttgttg cgtatctaaa
     8641 ttaaatgtca actcttcctc gaatgtttct tgtaattcct gcaaaatgct taggagactt
     8701 ttagattgta atgaagttaa agtagacagt tcatctagtt caatagaccg aatatccaat
     8761 aatatattta aaatggtaat tttatctgta attctttttt caatgtattt gtttagcata
     8821 gttaccgaat cttagttgca tatagataat tttaattatt ataatacaaa agaaactaat
     8881 tgtcttgtca aaaaggttgt ggaatttccg actttattga taaaacagca tgtaataaaa
     8941 ggc
//
LOCUS       DAVKLX010000069         8726 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKLX010000069 DAVKLX010000000
VERSION     DAVKLX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.069"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..479
                     /locus_tag="VW325_001924"
     CDS             <1..479
                     /locus_tag="VW325_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEU9735256.1"
                     /translation="NVDKKGYELICLQHPVSYDLRRIISVIKISTDIERIGDRIVEIL
                     KNLQIIQNNEILKKIISEIKILHEVIGLHMNRAISCYREEQSGCLDMVVIQKQNEIEE
                     LSINIEKKIMNYIFEDDGNVSEVIGALDIIHHLDKIAHTTQAIYKWIMYRKYGNIN"
     gene            492..1966
                     /gene="galT"
                     /locus_tag="VW325_001925"
                     /pseudo
     CDS             492..1966
                     /gene="galT"
                     /locus_tag="VW325_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
     gene            1979..2992
                     /gene="galE"
                     /locus_tag="VW325_001926"
     CDS             1979..2992
                     /gene="galE"
                     /locus_tag="VW325_001926"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9735257.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKIDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YNL"
     gene            3173..3832
                     /locus_tag="VW325_001927"
     CDS             3173..3832
                     /locus_tag="VW325_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9735258.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRVTEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVRFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3857..4924
                     /locus_tag="VW325_001928"
     CDS             3857..4924
                     /locus_tag="VW325_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9735259.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIPGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSISGLQGGTGRAILASILVRYLDDKGELGVSEKGWEAAKEYLKNAYTL
                     QKGESSIVKMLDKEDPIQYGMMWGSGALVGQKEQNVVFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQTEIQVEYSKNFGSIPANKDALKDLPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            5027..6037
                     /locus_tag="VW325_001929"
     CDS             5027..6037
                     /locus_tag="VW325_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9735260.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            6049..7741
                     /locus_tag="VW325_001930"
                     /pseudo
     CDS             6049..7741
                     /locus_tag="VW325_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            7744..8454
                     /locus_tag="VW325_001931"
     CDS             7744..8454
                     /locus_tag="VW325_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9735261.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            8550..>8726
                     /locus_tag="VW325_001932"
     CDS             8550..>8726
                     /locus_tag="VW325_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735262.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINS"
BASE COUNT         2983 a         1175 c         1691 g         2877 t
ORIGIN      
        1 taaatgttga taaaaaagga tatgagttaa tttgtttaca acatcccgtt tcctatgact
       61 taagaagaat tattagtgta ataaaaatat caactgacat tgaacgtatt ggggatagaa
      121 ttgttgaaat tttaaaaaat ctacagatta ttcaaaataa tgaaattttg aagaaaatta
      181 tttcagaaat aaaaatactt catgaagtta tcggactgca tatgaatagg gctatatctt
      241 gttatagaga ggaacaatct ggatgtttag atatggttgt tatacaaaaa cagaatgaga
      301 tagaagagct tagtataaat attgaaaaga aaataatgaa ttacatattt gaagatgatg
      361 ggaatgtttc tgaagtaatt ggtgctttag atattattca tcaccttgat aaaattgctc
      421 atactacgca agcaatttat aaatggataa tgtataggaa atatgggaac ataaattaga
      481 aagaaaaata tatgaaaaca attattgata attttgttga tagagtaatt gaatacggaa
      541 tgtataatga aattgacaaa atatatgtga aaaatagagt actagcgtta ataggtgagg
      601 agggaattga tcgcatttcc gatgaaaatg acctgaaaca aataaaagat tacttagtgg
      661 aaatcgcctt aaaaaacggt aaaattaaag atttaatcga ggaaaaggaa tgtttaggag
      721 cagagttaat gaatttcata gttcctttac ctagtcgatt aaatgatatt ttttggagtt
      781 catatgatat atcacctcaa gaagcagttg aagagtttta taagttaagt aaagatagtg
      841 attatattaa aacttctgct attgctaaga atattgagtt tcgagcatca actaaatacg
      901 gagagctaga gattacgata aatttgtcaa aacccgaaaa gatccaaaaa caattgctgc
      961 tgaaaaatta gtaaaagcaa ctaattatcc caaatgttta ctatgtatgg aaaatgaagg
     1021 atatcagggg cgaataaatt atccagcacg ttcgaatcat agaattattc ggttgaaact
     1081 tggtgacgaa gtatggggat tccagtattc tccctactca tactttaatg agcatgcgat
     1141 atttttgaat agtcaacatg taccaatggc tattacctct aaaacatttg aacaattatt
     1201 ggagattgtt gatattttgc caggatattt tgctggttca aattctgatc ttcctatctc
     1261 aggaggctcc attcttagtc ataaccacta ccagggaggg aaacatattt tcccaatgga
     1321 aaaagcaaaa tttgagagtg aatttcgttt taaagacttt gaggatgtta atgctggtat
     1381 tgtaaaatgg ccaatgtcag taattaggtt acaaagtgaa aataaaaatc gcttgttaga
     1441 tttagctatt aaaattctaa ataagtggag agaatattca gatttagaag tagacatcat
     1501 tgctatgact gaagatgtcc cacaccatac ggtgactcct attgctcgta aagtggacgg
     1561 gaaatatgaa ttagatattg ttctaagaga taatcatact actgaacaat acccagatgg
     1621 tgttttccat cctcatcaag atgtacaaca tattaaaaag gaaaatatcg ggttaatcga
     1681 agtgatggga cttgcgatat taccacctcg tttgaaacca gagttagaag aagttggaaa
     1741 atatctttta ggagaggata acgcaattgc ggattatcat ttagaatggg ctgatcaatt
     1801 gaaagaaaaa tatcctaaca ttaaaaaaga agaggttaat agcgtagttc aacacgaagt
     1861 aggacaagca tttgcgagag tcctagaaga tgcaggtgtt tataagaata cttcatctgg
     1921 acatgaagcg tttatgagat ttgttaaatc ggttggaata aattaggagg aaacaaaaat
     1981 ggcaatattg gtaacaggcg gagctggtta tattggtagc cataccgtag tagaattact
     2041 aaatttagga aaggaagtta tcattgttga caacctttca aactctagca tcttggtgct
     2101 agatcgtatt gaagcaatta caggaatacg tcctgtgttt tacgaattag atgtttgtga
     2161 taaacaagca ttgagaaagg tatttgaaca agaatcgatt gatgctgcaa ttcattttgc
     2221 aggttataaa gctgtcggag aatccgtgca aaagcctgtg atgtactaca aaaataatat
     2281 tatgagtaca ttggcacttg ttgaagtgat gtcagaattt aatgttaaaa agattgtctt
     2341 ttcttcaagt gcgactgtat atggaattaa caatcagtca cctctaattg agacgatgca
     2401 aacaagtgct acaaatcctt atgggtatac gaaagtgatg cttgagcaaa ttttaaaaga
     2461 tgttcatgtg gcagattcag aatggagtat tgcgttgctt cgttatttca atccaattgg
     2521 tgctcatgag tctggtttga ttggggaaga tccctcagga attcctaaca acttgatgcc
     2581 ttatattgca caagtagcgg taggtaagtt gtcagagcta agtgtgttcg gaaatgatta
     2641 tgatacgctt gatggtactg gtgtgcgtga ttatatccat gtagtagatt tagcaatagg
     2701 acatataaaa gctttagaaa aagtatctga aaaaatagat gtttatatct ataacctagg
     2761 ctcgggagaa ggcactagtg tattacaact tgtaaataca tttgaaagtg ttaataagat
     2821 ccctattcct tataaaatag ttccaagacg ttcaggagac gttgcaactt gttatgcaaa
     2881 tgcagacaaa gcatataagg aattaaattg gaggacaaca aaatcgattg aagacatgtg
     2941 tagagataca tggaattggc aatcaaaaaa tcccaatggc tataatttat gatactgtct
     3001 atatgagtgc tgtattttgg aaaaattact taaactttta atgaaatgca tatttaatga
     3061 aagataaatt tttttaaaaa atgtttgaaa gcgcttgaca aaggaaacgg tttcgttata
     3121 taatataagc atagatgaca ccggtgtcaa atataaaaaa gagggtcttt atatgaaaaa
     3181 cctaacaaaa attaagttca aagaaaatgg agaatttaat cattttccag gaaatactgt
     3241 tgtagcaaat ctttatacta aacaagattt gatggaagtt gttgatatta ttcaatcacg
     3301 ttatagagaa ttaccattta tcgataagtt tactttaact ccaaggaatt ctattcacat
     3361 gactgtaatt gaattgttgt gccatgaaaa tcgtgaaacg gagttttgga gcagtaatct
     3421 tcctcttgat acacctttac aggaaataca tgattacttt gctaaacaac ttgaaatttt
     3481 tccattgttg gatgaagaaa ttcatatgcg tgtaactgaa atgggaaaac aaaacatact
     3541 agttgaacct gcagatgaag cttctgcaaa gagattagaa gaaattcgta cttatgtctc
     3601 agaaaaagca ggtgttcgtt tccctaatca tgatagatat caattccata tttcaattgg
     3661 gtatcttcgg attcctctaa ccgaagagga ggaagaagag tttactaaag tcagagcaga
     3721 attaactgaa attttattag agaagattcc aactattact gtaaaccgta ttgactatac
     3781 tgtatttgaa gatatgagac aatttgttcc atatcatgaa aaatttaaat aaaataataa
     3841 aaaaggagaa cttatcatga aaaaaatgaa agtttggtct actgtacttg caacgggagt
     3901 tgctcttact acacttgctg cttgctctgg aggttcaaat tctacgactg cttcttcatc
     3961 tgaagaaaaa gctgataaaa gtcaagaatt agtaatctat tcgaactcag tctcaaatgg
     4021 tcgtggtgat tggttaactg ctaaagcaaa agaagctggt tttaatataa aaatggttga
     4081 tattcctggc gctcaattag cagaccgtgt tattgccgag aagaataatg cagttgcaga
     4141 tatggtattt ggaattggtg ctgttgattc aaataaaatt agagatcaaa aattactagt
     4201 ccagtacaag cctaaatggt tagataaaat tgatcaatct ttatcagata aagataatta
     4261 ttataatcct gtgattgttc aaccattagt tttaattggg gcgcctgatg taaaagaaat
     4321 gcctaaagat tggactgaat taggtagtaa gtataaaggt aaatattcaa tttctggtct
     4381 tcaaggtggt acaggacggg caattctagc aagcatctta gttcgatacc ttgatgataa
     4441 aggtgaatta ggtgtttccg aaaaaggttg ggaagcagca aaagaatatt tgaaaaatgc
     4501 atacactctt caaaagggag aaagttcaat tgttaagatg ttagacaaag aagatccaat
     4561 acaatatgga atgatgtggg gttctggtgc attagttgga caaaaagaac aaaatgttgt
     4621 tttcaaagtt atgactcctg agattggtgt accatttgta actgaacaaa ctatggtttt
     4681 aagcactagt aaaaaacaag cgttagctaa agaatttatt gattggtttg gtcaaacaga
     4741 aattcaagta gaatatagta agaactttgg atctattcct gcaaataaag atgccctcaa
     4801 agatctacca gaagatacta agaaatttgt tgatcaagtg aaaccacaaa atattgactg
     4861 ggaagctgtt ggaaaacatt tggatgaatg ggtagaaaaa gctgaattag aatacgtaca
     4921 ataagaaata agagttaatt tttatgtcca agtctctcta ttatttctaa atttatagaa
     4981 gaaataaaga gaggcttttg gtttagtaat agagggagtt agtgagatga ttaaatttga
     5041 taatattcaa attaaatatg gtgattttgt tgcaattgat aatctgaatt tagatataca
     5101 tgaaggggaa ttctttacat ttcttgggcc ttcaggatgt ggtaaatcaa ctactttgag
     5161 agcattggta ggttttttag atccatcatc aggaagtatt gaagttaatg gaacagatgt
     5221 cactcatttg gaacctgaaa agcgtggaat tggtattgta tttcaatctt atgcgctatt
     5281 tccaactatg actgtttttg ataatattgc atttggttta aaagttaaga aggtagctcc
     5341 agatgttatt aaagctaaag tatcagcagt ggcagcaaaa attaagatct ctgatcaaca
     5401 gttacagcgt aatgtatcag aattatctgg gggtcaacaa caaagggtag cattggctcg
     5461 tgctctggtt cttgaaccta aaattctttg tctagatgaa ccattgtcaa accttgacgc
     5521 aaaattacgt gtagatttga gaaaagagtt gaaaagactt caaaaagagt taggtattac
     5581 tactttatat gttactcatg atcaagagga agccttgact ttatctgata gaattgcagt
     5641 ctttaacaat ggatacatcg aacaggtcgg tacaccagta gagatttatc ataattctca
     5701 aactgaattt gtatgtgatt tcattggaga tattaatgtt ttgaccgatg aaacagtcca
     5761 cgaagtatta ttgaaaaata caagcgtttt cttagaggat aaaaaaggat acattcgatt
     5821 agagaaagtt cgattcaatc gtgaaactga acaagatttt attctaaaag ggacaattat
     5881 tgatgttgag ttttctggag ttacaattca ctatacaata aaagtttctg aaagtcagat
     5941 tcttaatgta acaagtattg atagtcaggc tgctattaga tctgtcggag aaagtgtgga
     6001 attatttatc acaccatcag acgttctgca attttaagga ggtgcagtat gcgtcataaa
     6061 ttaaatttaa aagattggct tattcgttta gggttaatct ggttcttagt aacatttatt
     6121 atttatccaa actttgatct agtagtgaat gtatttgtaa aaggaggaga attttccctt
     6181 gatgctgtac atcgtgttct aaaatctcag agggcacttc agagtattat gaacagtttt
     6241 aagttagcat tttcactcat tattacagtt aatgtcgtag gtattctttg tgttctattt
     6301 acagagtact ttgatattaa aggtgctaaa attttaaaat taggttatat gacctcttta
     6361 atttatggag gagtggtttt agcgactgga tataaatttg tctatggtcc ttatggattg
     6421 attacaaaat ttttacaaaa tgttatccct tctttagact ctaactggtt tattgggtat
     6481 ggtgcagtct tattcattat gacattttca ggaactgcta atcatacatt gtttttaaca
     6541 aatacaattc gaagcgttga ctatcacact attgaggctg ctcgaaatat gggagcaaaa
     6601 ccatttactg ttttccgaaa agtagtgtta ccaaccttaa ttccaactct atttgcactt
     6661 actattatgg tttttcttag tggtttatct gcagtagcag cacccatgat tgttggtggt
     6721 aaagaatttc aaactataaa tccaatgatt attacatttg cagggatggg gaattctcgt
     6781 gatttagctg ccttacttgc aattatttta ggtattgcaa ctacaatttt gcttactatc
     6841 atgaataaga tagaaaaagg tggaaattat atttctatct ctaagactaa agcgcctctt
     6901 aaaaaacaaa aaattgcgtc taagccttgg aatatcattg ctcacattgt agcatatgga
     6961 ttgttcacag ttttcatgct tccactaatt tttatagtat tatactcatt tacagatcca
     7021 gttgcaattc aaacaggtaa cttaacatta tcaaacttta ctttagaaaa ttatcgctta
     7081 ttctttagta atagtgcggc attctctcca ttcttggtca gctttattta ttctattatt
     7141 gctgcgacaa cagcaacaat tctcgcagtt gtatttgctc gtgttgtcag aaaacataaa
     7201 tctcgttttg atttcttatt tgaatatggt gctctacttc cttggttact accaagtaca
     7261 cttttagcag taagtttatt atttactttt aatcagccac aatttcttgt cttgaatcag
     7321 attttggtag gtagtttggt aattctactt attgcatata tagttgtaaa aatcccattt
     7381 tcttatagaa tggtacgtgc tattttattt agtgttgatg atgagatgga agatgcagca
     7441 agaagtatgg gtgcttcacc tttttatact attgatgaag gttatcattc catttatttt
     7501 accggttgtt ctctctgtta ttgctttaaa ctttaactct ttattaactg acttcgactt
     7561 atctgtattc ctttaccatc ccctagctca accattaggt attacgattc gatctgcagg
     7621 tgatgaaaca gcaacatcta atgcacaagc tctggtattt gtttatacaa ttgttctgat
     7681 gattatttct ggaacggtat tatacttcac acaaagaccg gggcgtaaag taaggaaata
     7741 atcatgacag ccactagtct tgggttatca aatattgaaa tagttgtcag gattgtttta
     7801 tcagtagtca ttggtagtat aattggttta gagagaggga gcaaatccca gcctgcaggc
     7861 atccgaactt atagtattgt ttgtctagct gcatgtttga ttatgatgac gaatgaatac
     7921 gtatcttata aatttgggac aggagatcct acacgattag gagctcaagt tatatcaggt
     7981 gtgggttttc taggcgctgg aacgattctt attacagata aaaagaaaat tacaggtctg
     8041 acaactgcag caggcatttg ggcttcggca ggaattggat tagctattgg agtaggtttt
     8101 tatgagggag ctcttttagt agccatttct gtttggggtg tgatatccat gttccaacca
     8161 ctaaaaaaat atctgcaaaa tcgttctaaa atgattgaat tgtatatagt ggttaaatct
     8221 acagaagcct ataatcgtgt attggtttat tgtgcagaaa atggtatcag aatgaccgat
     8281 tcaagaacag cgtttggaga tgttaattct gacagaattg aatactttga tgttccggac
     8341 aaaaaaatag cgtcatttat tactctggaa ttatcaggta gatttgaaca tcttcgactg
     8401 atggaagaaa ttgctaatat tgtaggtgtt atttatgtcg aagaaattag ctgaaattta
     8461 gtaaacaaag ggacagatgt ttttgtcctt ttgcttttta gatgttttaa tcatctaatt
     8521 tttgagtaga tttttgctca gaggttttta tgaaagatta taaaatcaat tttgatttag
     8581 gtaaaataga atattttgat aataattgtt tgatacaagt atacaaattt atttcttttt
     8641 atgatatttg tgaaatggtg tttgcctttc atttacctcc tgatgagttg ataacaaatg
     8701 ttatctttaa agaaaaaatt aactct
//
LOCUS       DAVKLX010000070         8468 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKLX010000070 DAVKLX010000000
VERSION     DAVKLX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.070"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(550..858)
                     /locus_tag="VW325_001933"
     CDS             complement(550..858)
                     /locus_tag="VW325_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9735263.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(855..1163)
                     /locus_tag="VW325_001934"
     CDS             complement(855..1163)
                     /locus_tag="VW325_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9735264.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(1168..2463)
                     /locus_tag="VW325_001935"
     CDS             complement(1168..2463)
                     /locus_tag="VW325_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9735265.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(2481..3890)
                     /locus_tag="VW325_001936"
     CDS             complement(2481..3890)
                     /locus_tag="VW325_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9735266.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKRS
                     GRWYRKVSDNNGFEVEIEE"
     gene            4048..4776
                     /locus_tag="VW325_001937"
     CDS             4048..4776
                     /locus_tag="VW325_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9735267.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            5235..5429
                     /locus_tag="VW325_001938"
     CDS             5235..5429
                     /locus_tag="VW325_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9735268.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRKLFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(5795..7105)
                     /locus_tag="VW325_001939"
     CDS             complement(5795..7105)
                     /locus_tag="VW325_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9735269.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            7342..8214
                     /locus_tag="VW325_001940"
     CDS             7342..8214
                     /locus_tag="VW325_001940"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9735270.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            8345..>8468
                     /locus_tag="VW325_001941"
     CDS             8345..>8468
                     /locus_tag="VW325_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9735271.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKL"
BASE COUNT         2642 a         1481 c         1598 g         2747 t
ORIGIN      
        1 tgatagagtt gtttacatct tatttcaatt cactatagtt taatttaaga gtagtattta
       61 ctaaggccca attaaaatca aagagcaaac gagaaaacga tgacaaggtg tgttgctttt
      121 tgatttctaa agagtataat gatagatctc tataaaataa gtgcgaagga aatgagcttt
      181 tataatcctt tcgttttaaa atactatctc agatattctt atatcgacaa gaagtttttg
      241 agtcattccc tcatcataca tattaaataa atagtggctc attcaatttt ttactagaat
      301 aataagctag tatagtaaac tgaaataaga tataaacaaa taaattggag cttaacatct
      361 atttccagca attttttaga aactgcagtg gactattcta aattcaacac attataaaaa
      421 ctagagtaaa agaaaaggat tggatcttgt gtaatgcagg atccaatcct ttcaatcatt
      481 ttgtccaact tttggaggtt cctacaatgt agccgtcatt aataaagaca gatgggaatg
      541 acagtgttcc tatttatttt gatagagatc gatgaattct ttagatagca actgaataat
      601 ctctgttgaa gccatttggt cttctgcatg cataaatagc aaggagaatc ctattttttc
      661 tccagtagct tctttttgta tgagattaga gtgaatcttg tgcgcttcta ctaaggagtc
      721 ttccgcttct tcaactttaa ttttcgcttc ttttaaattt cctgccttag ctagttggat
      781 ggcttcaata aaggatgatt tggctgctcc actattggca atgagctgaa aacagatata
      841 ttccatttct tctgtcatct tatttctcct atccatgcaa gtgcttgttc cagaactttt
      901 gctccattca tcattccgta atcccgcata tcaatggtat ctacagggat atttcctgca
      961 atttctttca cagcaagtaa ctcataacga atttgtggcc caattagaat gacatctgct
     1021 tcatggatat tctttttagc ttctgtcatt gattttgctt ggatagaaat ttcaatccca
     1081 cgttcagtcg cactttgttg cattttttta acaagcatac ttgtcgacat tcccgcatta
     1141 catactaata aaatttgttt cataatctta accttccatt tcttgttcaa caactttgtc
     1201 attaactttg ataaatggaa tgtatagaag aactccaagt gcaaagatga tgaattgaac
     1261 tagaactgct ctcacgtccc ctgctgttgc taaccatgca tttaagaata ctggtgtagt
     1321 ccaaggaact tgtataaatg caggactcat gaatcctgta actgttgcta agtagctgat
     1381 taaaatacca aggactggaa ctgtgataaa tggaatagct aatgaaatgt tataaacgat
     1441 tgggtaaccg aataatactg gttcattgat attgaagata ccaggtccaa aagataattt
     1501 agccacgttt ttagagacag cattgcgact cactaagaat gttgctatta ataaacataa
     1561 tgtagatcca ctaccaccca ttaaagcgaa tgtttgtatt tgtgataggt tgatgatgtg
     1621 tggaatggct tgtccattat ttgctgcagt gatgttttca gtaatgttaa ttaatagtaa
     1681 tggttctagg atggcactgt aaataactgc ttggtgaata ccaaatagcc ataacatatt
     1741 tcctaaagag taaataataa tgaccccgat taagcttgta ccaatatgac gaattggttc
     1801 ttgaataaag attgtaatga ttgagattaa gttcattcca gttatattga ataataatgc
     1861 tgaaacaacc ccaaataagg agatgacggt catgactgga agtaatacgc taaatgatct
     1921 actaacagct ggtggaatat tttcaccaag gttcatttgt aaagctttaa cgtttgataa
     1981 ttcaatgaat aattctgttg caataatcgc tacgataacc ccggcgaaca ttgcgcctgt
     2041 acctgtgttg ttgaatgaaa gaacacctga aatgtttacc gcatcttttg ctccgtcagg
     2101 aactacagaa actgtatttg gcatcatcac aattaaagaa actaatgata gcattgatgc
     2161 tgctaacggg ttttcgaaat ctctgttttt agctaagaaa taaccaacca ttacagcaat
     2221 aatcatacct gaaatactta aagtaccgtt tgcaattgtt attccccaat attggaatct
     2281 tgttaatgta tccccttgga aaatccactt aaataccgtg ttgttcaaaa gaacgattaa
     2341 acctgccaaa atatataatg gcattactgt tacgaatgca tctcttaggg tttttaaatg
     2401 aatttggttc cctagtttac cagcaaaggt ggcaaattga tctataaatt ttgagttttt
     2461 catctgaata tctcctctct ttactcctca atttctactt caaaaccgtt attgtcactt
     2521 actttgcgat accatcttcc tgatctcttg atggttctct tttgcgtttc taaatcaacg
     2581 gagataaatc cgtaacgatt cttatatgca ttattccaag accagcaatc aaatgcggtc
     2641 caagcgtggt atccaaaaca gtgacttccc tcttcaatgg ctttatgtag ccatcttaaa
     2701 tgttcttcat aaaattcaat acgatacacg tcatcgataa ctccattttc accgataaac
     2761 cgtgcttcgt tttcaacacc cattccgttt tcactgataa accatgggat attaccatat
     2821 tcttccttca caatcatagc aatatcataa atggctttcg gaaaaatttc ccaaccacga
     2881 tatggattca tgcgacgctc cggccattca tactctttga agtattggtc tggcatccag
     2941 ggcgtctgat attcctcagg atttgcttgt gcttgaacac gttttggatg gtagtagttt
     3001 actccaagaa aatcaaccgt atttgatttc atcagttgaa gctctttttc ggtatgactc
     3061 cataacacgc catctctctc tagctgtttt accaatcttt ctgggaaagt tcctttaaca
     3121 gctggattca agaagacttt gttaaagaag tcatctgtaa atcgacttgc ttctaagtct
     3181 tctggagaat tacttcttgg ataagcaggt gtcaagttta aaataatccc aatctttcca
     3241 tcaagtccta atgagcgata tagttgaatc acttttgcac tagcaaggtt tagattatag
     3301 atgacttgta cggcctcttt tccctttcct tttagatttg gataatggaa agcatataag
     3361 tatcctgctt ctggaatgac cattggctca ttgaaagttg tccagtaatg aaccttatct
     3421 ccaaagcatg tgaaagcagt cttggcaaac ttcacgaata actccactac atgtttgctt
     3481 tcccaaccac cgtatttttg aagaagttcc actggtaaat caaaatgatg taaattcatc
     3541 acaagatcca tctggttctt tttagcttct tcaatgatgg cattgtagaa agcaatacct
     3601 tttggatcag gctcacctgt ctctaaattc ttgatgagtc gactccattg gatggaagtg
     3661 cggaaagaat taactccaat ttccttcatc aagtggaaat cttcggtgta agtatgaaaa
     3721 aagttactgg ctacaagagg tccgacatta tcgaaaaaat cttctggatg cgttttgaac
     3781 cagtagtcca tcacattttc atgtactttt ccatattgac cttctgtctg aggtccgcta
     3841 gaagcagctc cccaccaaaa cagattagga aatactaact tttttttcat gctcaacctc
     3901 ctttctatta ttctatacat aaagtatagc ataattttaa aatttgtcta gacattttta
     3961 aaaattgttg atacatatct tgtgtttgcc tcatttcttg aactttgtta tactactatt
     4021 agaatattat agagagaacc agattaaatg agtacaaaat atttatttat ttacaatgag
     4081 attcgtgaaa agattctttg taataaatat accatgaacg aacaattgcc tgatgaaatg
     4141 acattagcta aacagtttgc ctgtagtcga atgacgatca aaaaagcttt agacttgtta
     4201 gtttctgagg gcttaatttt tagaaaacgt gggcagggaa cctttgttct ctctcgtggc
     4261 agctcaaaaa gaaaattaat cgttccagaa agagatatcc ggggattgac aaaaatatct
     4321 gaagatgctc attctacaat tgactcgagg attattcact tcaaattaga atttgcaaat
     4381 gaatttttag cagaaaaact acaggtcgct ttgcagagtc cagtttataa tatttaccgc
     4441 ctgcgtatta ttgacggtaa accttatgtt ctggaacaaa cttatatgag taccgatgtt
     4501 attccaggta ttactgaaga tattttacaa aaatcgattt acaattacat tgaaggaaag
     4561 ttaggattgc atattgccag tgctacaaaa atcttgcgag cttcttctag ttcagaaaat
     4621 gagcaacatt acttacagct ccttccaacg gaaccggtat ttgaagtaga acaagtggct
     4681 tatttggata acggaactcc gtttgagtac tcgattagtc gtcatcgcta tgatttattt
     4741 gaatttaatt cttttgcatt acgacattcc tcctaggaga aaatgtgaaa atgaagccaa
     4801 tcttttacag actctagttt aagaaaaatt taaaacaggg caagaaggtc ccatctatgc
     4861 ttaaatggtt tctcttttct aaataagatg gctttaaaag agtgatcgtt gtatccatca
     4921 tgttgaaaaa tatcttcgta tagcttatag agtaggtact gaaattgttc acctgatcta
     4981 cttcttatag ttatttagtt ttaaatagtg tttcaaacat tcttacactg acgagaagtt
     5041 tttgagtctt ttcttgtaac acatatagta tactgtggtt agaatagtag actgtgactt
     5101 ctaacaaatt gctagaaatg aatttcaatc tcccaattta tttgttcata tcttctttta
     5161 atatattaaa taaattctaa atcataatca tttaaaaaaa ttttattttt tatttttcat
     5221 tacgaataat atagatgaag gggaaagagt atgaaaacag aactgtttct tttgctatta
     5281 gttcaaaagg agaaaaaatg aaagtagaaa atatttcgta tagggtggat catcgtaaat
     5341 tgtttgataa tatttctttt gatacttcga gttcagacgt gacattaatt actggtaaaa
     5401 atggtacagg aaagtcaact ttactatagt agattgaaac tagaatagta cacatctact
     5461 tctaaaatat tgttagaaat cgatttgact atcctgatct atttgtcctg ttcttatttc
     5521 atttcactat atctcaaatt gagtatgacg aagtgcgctc ccatgtcctg ggaacgcact
     5581 ttcttcatat ttttcatatt cttgaatcca tcgataaaga ctattgggat gaatttttaa
     5641 agttgaacta atcattttta caggatgaga tttacagcag agagtttgaa ggctttatca
     5701 aaggtttttc ttggcataat gacttttcct cgtttccact taattttgtg tctactttat
     5761 tataccaagt ccactcttaa gttagataat aaatctaact taaggaagct agaaggatga
     5821 gaatccaggt ggtcaagagt cccaaactta agctgatggg gacacccaga ataatttgct
     5881 ttttgaaggc aaggccacgt tcctctatat tgggaagtga gagttgaatg agagaaccag
     5941 ctgatgaaaa gggtgagata ttagtagata gagcgccaat aacggtggct gttgtgagta
     6001 agtgaatatc aatctgagga ttttgagcac tgatgatagc aatgatggga aagagggctg
     6061 gagctacaac ggatagggtg gaactaaaga gtgacatcac tccggctatc acacaaaaga
     6121 acagaggtaa ccagaaatga ggaatggttg ttgtcatgag gtgccctatc agtgtgacta
     6181 aacctgactt gaccgctaga gacattagta agctcatgcc gcagagcatg ataattgtag
     6241 cccagggaac cttagctaaa atggcttctt gcttccctaa tttgagcctt aaggcgaggc
     6301 agaccatgag tattgagaca aagccaatat caaatgtttt ttgataagta gctatccagg
     6361 cgatgtttgg gaaaatgaga tgcaacaagg gaaaaagcca aaccaaaacc atgctgctga
     6421 tcatgagcaa ggtggtttgt ctttgaacct tgctgaggag tggtggttgg tcaatagtca
     6481 aggatgagtt tgttcttccc ttactatagt gactgtaaca ggataataaa agcaagacga
     6541 tgagtgggta gataatgctg acgataaaga tatgattgcc aagtgaaaaa gcttgctctt
     6601 cccatcccat ttgcttaaac aggccttgaa agacaatgcc tgagctactg gttatcaaat
     6661 tagcccctcc tgaagctccc caattgacgg cttgagctcc aatcaaaggg tgtttgtccg
     6721 ctttttgaca gagggtaatc gctagaggac agcaaacggc catagtagtg aaaaatccag
     6781 cacctaaagc agacaaaagg gttgccatca ggtataaaat catgtagagg gcgttagggt
     6841 gggtgcgtgt gcggtagaga atgtgttgag ccaaaacatc aagagtaccg ttagttgttg
     6901 caacgttata aaagagagag acgctaaaaa tggtaaaaaa gagtgaggtt ggccaaaaat
     6961 gaagaagttc tttggggctt aatcccatga gagtggttgc gatgaggtaa gaaaaagcaa
     7021 tagccagcag gccaatattg attttggtgc ggtaaccaat tccaatggct agagcaatgg
     7081 cgctaatcat tattaaatga atcattgaat tgtcctttca gttagaatat ggaaataggt
     7141 tggcaatttt ggcgctcaaa agaccacctg tttcctattg gtacttgact attatacaca
     7201 aaaaaaatac aattgtcttg acaattgtat tgacacttgt tagaattaaa aatgaggtat
     7261 tcaggcttgt gatttcctat ggaagttaat agtaattgcc tctagtgctt acaagtgata
     7321 ttaaaaatag aggacttagt gatgtcaatc atttcaactg atttaacccc ttttcaaata
     7381 gatgatacat tgaaagcagc cttgcgagaa gatgttcatt ccgaagatta cagtaccaat
     7441 gccatttttg atcatcatgg ccaagccaag gtgtcgcttt ttgccaagga agctggtgtt
     7501 ttagcgggac taaccgtttt tcaaagggtt tttaccctat ttgatgccga ggtgaccttc
     7561 cagaatcctc atcaatttaa ggatggggat cgtttgacta gtggcgattt ggttttagaa
     7621 atcataggct cggtgagaag tctcttaaca tgtgaacgcg ttgccttgaa ttttttacaa
     7681 catttatcag ggattgcttc gatgacagct gcttatgtag aagccttagg cgatgattgc
     7741 attaaggtat ttgatactcg aaaaactact cctaatttac gtctttttga gaaatatgcc
     7801 gtgagagttg gcggtggcta taatcatcgc tttaatttat cagatgctat catgctaaaa
     7861 gacaatcaca ttgcggcagt aggtagtgtt cagaaggcaa ttgctcaagc gcgtgcctat
     7921 gccccttttg tgaaaatggt cgaggtggaa gtggaaagcc ttgctgctgc cgaagaagct
     7981 gcggcggcgg gtgctgatat tatcatgttg gataatatgt cattggaaca gattgaacag
     8041 gccattaccc taattgcagg acgttctcgg attgaatgtt ctggaaatat tgatatgacc
     8101 actattagcc gttttcgtgg tttagcgatt gattacgtct ccagtggtag tttaacccat
     8161 agtgctaaga gtcttgattt ttccatgaag ggtttaacct accttgatgt ctaagttgta
     8221 aaataaacta actttttaaa ggatgtcttt cctctagaac gagttttatg tcagatagtt
     8281 taaacgcctc ttcaaatata gtaaaatgaa ccaaaaatag tacacaatgt ggtataatct
     8341 tcttatggca tattcaatag attttcgtaa aaaagttctc tcttattgtg agcgaacagg
     8401 tagtataaca gaagcatcac acgttttcca aatctcacgt aataccattt atggctggtt
     8461 aaagctaa
//
LOCUS       DAVKLX010000071         8354 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKLX010000071 DAVKLX010000000
VERSION     DAVKLX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.071"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..956
                     /locus_tag="VW325_001942"
     CDS             <1..956
                     /locus_tag="VW325_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9735272.1"
                     /translation="FASLLGKAPQTIHTEIKRGTVRQCLGKGRFKEVYSADYAQQSYE
                     NNRKRSVKKSSLTKELKEKILHYHNQKFSPEMMVMAKGVNVGISTIYYWIHHGKLGLS
                     KQDLFYPRKGKALKKQASTNFKPAGQSIEQRSEAINLRLENGHYEIDTVLLTRAKNYC
                     LLVLTDRKSRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSE
                     EHIYYAHPYASWERGTNENHNRLIHRWLPKGTKKMTPKEVAFIEKWINNYHKKCLNYK
                     SPREDFLMANLNLKFSVRNKSRNRFKFCRSFKYPARRCSWC"
     gene            928..1572
                     /locus_tag="VW325_001943"
     CDS             928..1572
                     /locus_tag="VW325_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9735273.1"
                     /translation="MLRAGVLGANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(1614..3602)
                     /locus_tag="VW325_001944"
     CDS             complement(1614..3602)
                     /locus_tag="VW325_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9735274.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVYFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3604..4362)
                     /locus_tag="VW325_001945"
     CDS             complement(3604..4362)
                     /locus_tag="VW325_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9735275.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVMTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4790..4981
                     /locus_tag="VW325_001946"
     CDS             4790..4981
                     /locus_tag="VW325_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735276.1"
                     /translation="MKHKEHILIGFLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(5335..5607)
                     /locus_tag="VW325_001947"
     CDS             complement(5335..5607)
                     /locus_tag="VW325_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9735277.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5666..6115)
                     /locus_tag="VW325_001948"
     CDS             complement(5666..6115)
                     /locus_tag="VW325_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9735278.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQRYQRKRRIDTRRYRCGLCRGKLLLINQPED"
     gene            complement(6102..8231)
                     /locus_tag="VW325_001949"
     CDS             complement(6102..8231)
                     /locus_tag="VW325_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9735279.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVNPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESETFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNINTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
BASE COUNT         2527 a         1763 c         1627 g         2437 t
ORIGIN      
        1 aatttgcctc tctacttgga aaagctcctc aaactatcca cactgaaatc aagcgtggga
       61 cagtccgaca atgtcttgga aaagggcgct tcaaagaggt ttattctgcc gactatgctc
      121 aacagtctta tgaaaacaat cgcaagcgct cggtcaagaa atcaagcttg accaaggaac
      181 taaaggaaaa gattctccac tatcataacc aaaaattttc gcctgaaatg atggtgatgg
      241 ctaaaggggt taacgtggga atttcaacca tttactattg gattcatcat ggaaaattgg
      301 ggttaagcaa acaggatctg ttttacccta gaaaaggaaa agcgcttaag aaacaggcta
      361 gcaccaactt taaacctgct ggtcaatcca tcgaacagcg gtctgaagct atcaatcttc
      421 gcttggagaa tgggcattat gagattgata cggttctact tacgagagcg aaaaactact
      481 gcttgcttgt cttgacggat cgaaagagta gacatcagat catccgattg attccaaata
      541 aaagtgctga ggtggtcaat caggctctaa aactcatctt aaaacaacac aagattcttt
      601 ccatcacggc agataatgga acggaattca atcgcttgtt tgatgtattt tctgaggaac
      661 atatctacta tgcgcacccc tatgcctctt gggaaagggg aactaatgag aatcacaaca
      721 ggctcattca tagatggtta cctaagggaa ccaagaaaat gactcccaaa gaagtcgcat
      781 tcatcgaaaa gtggattaac aactatcata aaaaatgctt gaactacaag tcacccagag
      841 aagacttctt gatggctaac ttgaacttga aatttagcgt cagaaacaaa tcacgaaatc
      901 gattcaaatt ttgcaggtcg tttaaatatc ctgcgcgcag gtgttcttgg tgctaacgat
      961 ggaattattt ccattgctgg tgtggttatc ggggttgcca gtgccacgac caatatctgg
     1021 attatctttt tatcaggatt tgcggctatc ttagctggtg ccttttcaat ggctggtgga
     1081 gaatatgtat ccgtttcaac tccaaaagat accgaggaag ctgccgtttc gcgagaaaaa
     1141 ctcttgttag accaagatag ggaactagcc aaaaaatccc tctatgctgc ttatatccaa
     1201 aatggagaat gcaaaacttc tgcccaactc ttgaccaata agatctttct taaaaatcca
     1261 ctcaaggctc tggtagagga aaaatatggg attgagtatg aagaatttac caatccttgg
     1321 cacgctgcca tttctagctt cgttgccttt ttccttagaa gtttgcctcc aatgctgtca
     1381 gtgaccattt ttccaagtga ttaccgcatc cctgctaccg tccttattgt cggtgtggcc
     1441 cttcttctca ctggttacac aagtgctaga cttggaaaag ccccaaccaa aacagctatg
     1501 attcggaacc ttgctattgg tctcttgacc atgggagtta ccttcctgct cgaacaactt
     1561 ttcagcattt agaatacaag aaatacctcg attttgaagt cgaggtatct tttttacatt
     1621 tgcacaatct tgcgataact tcttgaagta atcatgaaaa tcagcacata ggcgatgagg
     1681 aagatagcgc agatagacaa ggtcacaatc aacatcatag tcgtatccag tacaccaatc
     1741 acttttaaaa tcagactaag catatggtag gcaaaggcta gatgtaggaa ggcaaagagc
     1801 aaaggaagga agtatacagt taaaacctgt ttgttgatgg tttgcttgat ttgcttttga
     1861 tctaaaccga ctttctgcaa gataataaag cgctcacggt cttcatatcc ttcagaaatt
     1921 tgtttgtagt agatgaccag aacggttccg accataaaga taatggataa gaaaataccg
     1981 ataaagaaga caccaccaaa gagggcactc atctgagcac tagcatctgc tagagtgcta
     2041 ccatacacat agttaccttc agtgtttaat tgagcattaa acttttgtaa gtatttttca
     2101 tactcctcag cgaccttgag ttgttctgct tcactggcat ttacattcat accaccgtaa
     2161 aactgattat agatagccga atctgggaat tgatccaaaa aggcttgtaa atcaggtaca
     2221 acaaggtaat tgtaatcagc agtcaaaata ttaaactgat ttggaacatg gttgacaata
     2281 aaatctttag taaattcttc ttttacagaa aattgatgat catttagagt tagagctttc
     2341 tgttctttaa ctccctcatt ctttgcaaag agtccgacct catttcctga tagagacagt
     2401 ttttgaccag tcatattttc ataatctttt tggtcaaata ccatgaaaac tgtttttggt
     2461 tggacacggt tttgtccttt ttcaaaaata gttaacttgg ttccttcttg attcgcaaca
     2521 gcaaagtaag tgtaacgaaa tacttctttt tctttaatct tataaccttt gtcacttgca
     2581 aactggctca agagtttgtc caagtcttct ttttcaacat tttgccctga aaccccaaaa
     2641 tcatgaggat ttagaacttt tttaaagctt tctgaggaat tgaaaatgct tgtcgctgct
     2701 gacatggtta ccaaaaccat tgttgacaag atagcgatgg tggctagtcc aactgcattt
     2761 ttcttcatac ggaaaatcaa gttagaaaca gatatgagat tatttggttg gtaatagtat
     2821 ttcttatttt tctttaagat ttggaggaaa acggtaatcc ctgcattaaa caagagataa
     2881 gtcccaaaga taaccagtaa aacagctatg aagaaggttg ttaaggctgt aagaggatct
     2941 tttaccgtaa gggcaagata atagccaatt cctaaactta tggaaccaag aatggtttgg
     3001 agaggaagga agcgaccttt tttctctcca ctagctttct cacgagagag ctggagggca
     3061 ttcatacggg cgattcgaag ggcattcagg aacatgaggc ctaggaaaat caaaccgaag
     3121 acaacaagca ctgtaatgac aactttcgtc tggaaggtag caaccagctc aaccttcaat
     3181 ttcattagtt tgagcaggaa agcgaaaatt aacttgtcaa acaaggctcc aataccgata
     3241 cccgctccaa cagttagaat cccaaatacc actaactcct taaaggtcat actgataaga
     3301 tgacgcttct ccaagcccaa catgccataa attcctagtt ccttggaacg gttcttcatg
     3361 acaaaactat tggcatagag aacgataatg gctgacgcaa gggtgacgac aaacatacca
     3421 aatccaagtg tagcctgaat ggttgttcct ccacggattt ccgcaatctt aggattgaag
     3481 gttagagagt aaaagagata ggtgacagtg actgccaaga gaacagccag cgcaaaagga
     3541 tagtagagtt tgcggttttt aatcaagttc gataccgcta acttattggt taatcgaaac
     3601 atactaattc acctcgcttg ccatgacagt caaggtatca gagatttctt ggaacatctg
     3661 acgctctgtc ttctctccac ggtagatttg gttgtaaaga atgccgtctt tgataaagag
     3721 aacacgcttg gccctgctag cagctgctgt tgagtgggtt accatgagga tggtttgccc
     3781 acgctcattg atttcattaa agacatcaag taaggctgca gatgacttgg aatcaagggc
     3841 tcctgttggc tcgtccgcaa ggagaatttc aggttctgtg atgatggcgc gggctactgc
     3901 tacacgctgt ttctgaccac cagaaatctc gtaagggtac ttctcttgca attggttgat
     3961 acccagattt tcagctgtca tcaccaattt cttcatcatc tccgttatag gtcttcttga
     4021 caagacaagc ggaagcaaga tattgtcctt aacagacaga gtatctagca agttaaagtc
     4081 ttggaagaca aatcctagct tttcacgccg gaaactagaa gcctgtgaat ttttaatagt
     4141 tgctgtatca gttccattta agtaaacctg accacgactt ggtttgtcca gcatagctag
     4201 gatattgaga agagtggatt taccagaacc agactcaccc atgatggcaa catagtcacc
     4261 cttttctacg gtaaagtgaa tatccttgag ggcttctact tggttgccct gaaaacgtgt
     4321 tttataaatt ttttgaacgt gttttacatc taaaagtgtc atgagaatct cctttcttaa
     4381 tatcccataa aatgaaatgt agcttgcatc atagtgcatc ccagctgaac gcgctcaatt
     4441 tctttgtgac ttggttttct agttttctta tgtacgattt gtttcatgat gttactcctt
     4501 tgttctttat gagtctagtt tacatcaaaa aaagaccgct tgccataacc taaccttaca
     4561 aaagagactt taatcttaca tttttgtaag attgggggaa atataagcaa aattacttag
     4621 aaaatgattt gatttcctat ttttaaagtt gtaaaatata agtgcgaaaa ggcttacttt
     4681 gaaaaggagg aagcgggaac taacatttta gtaaggttac ctagatgaca caccattaaa
     4741 ttgaacactt tcaaatcgaa ctacctataa ggaaagaagg tgcgctttaa tgaaacataa
     4801 agaacatata ctcatcggat ttctctatct gctctctcca ttcatcggtc agctcttagt
     4861 tgaacacaca cactttatcg gtacagaatt taccggcact gcttatgtta tttgctggtt
     4921 atcagttgtt atcagcatcc accatttttc caaaaatgta ttgtctcagc agcaaaaata
     4981 gattaaaaaa ctgagtttaa attactgctt ttatagtaaa atgaaataag aacaggacaa
     5041 atcgatcagg acagtcaaat cgatttctaa caatttttta gaagtagagg tgtactattc
     5101 tagttccaat ccactatagt ttgtagatga agctggcgaa atattattaa tactattcaa
     5161 aaatcaaagt gcaaactata tcaacttcac attgccgact aaattacagc ctgcggctgg
     5221 cttcctagtt tgcactttga tttttattga gtataaaagt aaaaaagctt taaatcaagc
     5281 agtttagact attgatttaa agctttttgc gtattccaaa tcttttgtaa aacctcacca
     5341 aaaccagcct tcattatcgt caatggcttg agcttctttg cgtttacgtg ggcctgttcc
     5401 gtacatttct gcttcgattt cttccttggt tggcatgata gaagctagga tgatatagat
     5461 aatcacccca agtccaaagt ttgcgactgt aaaaatggca aacagaaaac gtacaagggt
     5521 aacatcaaag ttccacttgt ctgacagacc tgccaaaact cctgaaatca tgcgatttcg
     5581 tctcatttta taaaatttac tgttcatgat atttcctctt tctgctaggt tagacatagt
     5641 atatcacggg taggctcggc tttcatcagt cctcaggctg atttattagt agcaatttac
     5701 ctcgacaaag tccacagcga tagcgtctgg tatcaattct tcgcttacgc tgataccttt
     5761 gctggcagga ttggcaacga tagagcttga ttggcttgga gttactattg ggcaaggatg
     5821 gtacaaaccg taatccatcc actgctttca acagttcctt aaaatcccga tccttatgtt
     5881 gatagccttt cccttgaaaa tagaggtgat aatgacagag ttcatgtcga acaattttcc
     5941 taaaaacgtc caaccccagt tcctgataaa ccttgggatt aaaatccaaa tgcccatctt
     6001 tggggaaaaa tcgcccacct gtcgaacgta gacgcctatt ccactggaca tgatggataa
     6061 aaggtctgcc gaagtcttct agtgaaacct gcttgacgta atcagtcagt ttcatttgga
     6121 gctaggagag acagattaac tttttcacgt tcagtatcaa ttttcttaac ccaaacgctc
     6181 accaaatctc caactgccac cacttgacta gggtgtttga taaacttgcg actcatatgg
     6241 gaaatatgga tgagaccgtc ctcatgaatt ccgatatcaa caaaagcacc gaaatcaaca
     6301 acgttacgca ccactccttc tagcttttgt ccaaccacta agtccttgat atctaggaca
     6361 tcttgacgga gaactggagc atcaaaggaa tcacggaaat ctcgacctgg tttgagaaga
     6421 tctgcaatga tatctttaag agtttctgga ccaaggtcta actcttgcgc catttccttg
     6481 actgaaagcg acttgagttt gctttgggcc tcttcattca ggtccttaat gtccaagcgt
     6541 ttgaagagtt ccttaacggc agcgtaattt tctgggtgaa ctcctgtatt atcaaggata
     6601 ttgctacttt cggggatacg aaggaaacca gctgcctgtt caaaggcctt agcccccaaa
     6661 cgaggaactt tcttgatttg ggagcgtgaa gtgatttttc cttcctcctc acggtatttg
     6721 acaatatttt cagaaatggt tttattgagt ccagccacgt gagaaagaag agctggactg
     6781 gccgtattga tattgacacc gacttggtta accaccgtat ccacgacaaa gtccagactc
     6841 tcagatagtt tcttctgact gacatcgtgt tggtattgac cgacaccaat tgacttagga
     6901 tcgattttga ccaattcggc aaggggatct tgcaaacgac gggcgataga aatagcagag
     6961 cgtttttcaa cagtcaagtc tggaaactcc tgacgagcaa gttcactggc agagtagacc
     7021 gaagcaccac tttcatttac gatgacataa ctaacctcag gaaaatcttt aagaacttcc
     7081 gccacaaagg tttcactttc acgactagca gtaccatttc cgatggcaat aatctctaca
     7141 ccgtattgac caatcaagtc cgccaaatct ttcttggctt cttcgatttg acgagctgat
     7201 gctgggttga caggatagat gacctgagtt gtcagcattt ttcctgttgc atccacgaca
     7261 gctaacttgg cacctgtacg aaaggctggg tcaaacccaa gaaccacgcg ccctttcagt
     7321 ggagcaacca agaggagatt gcgcaaattg tcagaaaaaa gttggatagc tccctcttca
     7381 gctttctcag ttaattctgt ccgaatacga cgctcaatag caggcaagac ctttttctta
     7441 acggattgct gaacaacttc atcaatataa gcatttttca ccttgaaacg agtagcaaag
     7501 aaggcaagaa tacggtccgt cgcatgttca aaaccgatct tcaagacacc aagtttctcc
     7561 ccacgattga gagccaaggt acgatagcct tgcatagttc caactgtctc tgaaaaatca
     7621 taataaatct gaaaaacctg cttttcatca agactttcat ccttggcttg agaagtgagt
     7681 ttagagtgtc tcagcacttc ctgataagtc atagaacgca aggtcacatc ttccgataag
     7741 gcttcgacca aaatatcaac tgcaccggtc aaggcttcct tgccagtcgc aaatccttca
     7801 cagacgaact tttcagcttc tttctctaag tcaactatat tctgcaaaat caagcgagca
     7861 agaggaaaga gtccagcttc acgggcaatg gttgccttgg tacgacgctt ttccttataa
     7921 ggaagataga gttcttcaac gtctgctaat ttttcggcaa ctaagatagc ttcttccaat
     7981 tccttggtca acttgccttg ctcttgaatc ttagctaaga cagcttcctt acggtcattg
     8041 agatttgtca gacttttatc caaatcaata atagccttaa tcgccacctc atccagacta
     8101 ccagtcatgt ccttacgata acgcgcgata aagggaatag tcgccccttc agctgtcaaa
     8161 cttagaacgg tatcaatttg ctttaacgtc actcccaaat cctgagagat tttttcatat
     8221 tttttatcca taaatctatt ataccacaag ctggaaggct ttctccaatc cttttcatac
     8281 tcaatgaaaa tcaaaaagca aactaggaag ctagccgtag gctgctcaaa atactgtttt
     8341 gaggttgtgg ataa
//
LOCUS       DAVKLX010000072         7199 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKLX010000072 DAVKLX010000000
VERSION     DAVKLX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.072"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(162..965)
                     /locus_tag="VW325_001950"
     CDS             complement(162..965)
                     /locus_tag="VW325_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9735280.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(1049..1261)
                     /locus_tag="VW325_001951"
     CDS             complement(1049..1261)
                     /locus_tag="VW325_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735281.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            complement(1740..1949)
                     /locus_tag="VW325_001952"
     CDS             complement(1740..1949)
                     /locus_tag="VW325_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735282.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            2119..2391
                     /gene="rpsP"
                     /locus_tag="VW325_001953"
     CDS             2119..2391
                     /gene="rpsP"
                     /locus_tag="VW325_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9735283.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            2411..2650
                     /locus_tag="VW325_001954"
     CDS             2411..2650
                     /locus_tag="VW325_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9735284.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            2918..3122
                     /locus_tag="VW325_001955"
                     /pseudo
     CDS             2918..3122
                     /locus_tag="VW325_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            3226..4014
                     /locus_tag="VW325_001956"
     CDS             3226..4014
                     /locus_tag="VW325_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9735285.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            4045..4563
                     /gene="rimM"
                     /locus_tag="VW325_001957"
     CDS             4045..4563
                     /gene="rimM"
                     /locus_tag="VW325_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9735286.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            4553..5272
                     /gene="trmD"
                     /locus_tag="VW325_001958"
     CDS             4553..5272
                     /gene="trmD"
                     /locus_tag="VW325_001958"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9735287.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGSQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKILVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            5284..5622
                     /locus_tag="VW325_001959"
     CDS             5284..5622
                     /locus_tag="VW325_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735288.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(5653..5973)
                     /locus_tag="VW325_001960"
     CDS             complement(5653..5973)
                     /locus_tag="VW325_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735289.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            6071..6286
                     /locus_tag="VW325_001961"
     CDS             6071..6286
                     /locus_tag="VW325_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9735290.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(6355..6435)
                     /locus_tag="VW325_001962"
                     /pseudo
     CDS             complement(6355..6435)
                     /locus_tag="VW325_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(6491..7027)
                     /locus_tag="VW325_001963"
     CDS             complement(6491..7027)
                     /locus_tag="VW325_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9735291.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
BASE COUNT         2314 a         1243 c         1499 g         2143 t
ORIGIN      
        1 tctttctaat gagttgtttt gtcgcttttc attataggtc atatgggact ttttttctac
       61 aataaaatag gctccataat atctatagtg gatttaccca ctatagatat tatagagcca
      121 gaaatacagt accgaatgtg gatactgtat ttttttggtt tttaagattt aaaatcgtag
      181 tctttcacca cttcgatgct gtcgattgtg atagcagtag ttggcttgtc tttttcatct
      241 ttttcggctt tagcaatctt atccacaaca tccataccgt caatcacttg accaaagact
      301 gggtgtttgc catctagact agggtttcca ccttctttgt aggcttcgat aattttctgt
      361 ggatacttgc ttgtagggag tttagaagag gtatctgtag agttttggtt gatgaagaac
      421 tggctgccat tggtgtttgg ttgaccagta ttagccatag caagagcacc acggatgtta
      481 tacaaataag gagtaatctc gttcttgaaa ccagttcctt tgtctttagt cttatccttg
      541 tcatgccata tggactgacc acctgtaccg tccccttttg gatctccagt ttggaccata
      601 aagccatcga tgacacggtg gaaggtaata ccgttatagt agccttcttt ggcgtgagtg
      661 aggaaatttt caaccgctag aggagcgagt ttagggaaga gtttaatgcg aatatcacct
      721 tggcttgtgt ggagaatcac ttcggcttca tcttcagcaa cttccttaga tagttgtggg
      781 aaattggcgt tttcgtttgt taaagcatcg tttaactcct tggcagattg ggcagctact
      841 ttggaacttt cttcagcagc aagactggaa tcaacataat catcaccacg cagactgcgt
      901 tggacgctgc tacacccagc tagggctaca gtagacagta aaagaagggt tgctagtttt
      961 ttcattctga tcctctcaaa aattcattta taaccattgt accctaaaag gaagtggatt
     1021 tcaaatatta gtagtttaca gaactggctt agataatagt tgggatttca tcatcatctt
     1081 gaaaatcttc aacttcttcc ccagtaaatg gtttctgaaa agaacttagt ataaaacggg
     1141 caattggttc aacaattagg aaatttagag gaagagctgc aataaagttt acgaaccagg
     1201 cgcttagata ggtattgggt gtgatagcaa tgccattgta aaaaagtcca tagaaggaca
     1261 tgaaagtgac cagctaattc ttcttctgaa aaatgctcat gaagggcttg atccaggtcg
     1321 caagatttca ttttagaaag tccagtaact cccttcttct tcaggatatt ctttttcatg
     1381 gtagtattta gatggtcaag tgagtcaaaa gaagtttcga caagagcata ccctttttct
     1441 accagagttt ttagatgttt aggggcatca ataccataag tgtactcaaa gtattttgga
     1501 aaccagatct taagaaagct cgtaaagcta gccagttctc taaacgctaa gaactagcta
     1561 caacacagca tttacaacac ttcatggttc acaccatggg gtgtttttta atgattttaa
     1621 gcaaaattca cacttaattg aaagttattt aaattagatg aaaatttatt ttttgaaaaa
     1681 gtgggaaaac attgattcta agcgaatttt gaatcatatt cgaatgggag tttttaaaat
     1741 catgtttcag tggtaaaagt accaaactgg attctccata gtaaaatgat atctcctgct
     1801 agtgaatcat ctactaataa gtccatattt cgcttgggaa cgggcttggg acttagaagc
     1861 caagtatcca actctcgttc aggcgatatg aagggtttga cttggtgttc tttataaaat
     1921 ttttctaaaa tagtattcac ggatagcatc tttcaaacat ttatttccta ttctaacaaa
     1981 tttctaaata ctttaagaaa tttatagtct gtcaagtttt tttcttgaca catgccagaa
     2041 atctgctata atagaacatg tgctagatag ctcagctatt tcaccgaaat aaaaaattaa
     2101 agaaatgaga ctttaaaaat ggcagttaaa atccgtttga ctcgtatggg ttctaagaaa
     2161 aaacctttct accgtatcaa cgtagcagat tcacgttcac cacgtgacgg acgtttcatc
     2221 gaaacagttg gaacttacaa cccacttgtt gctgaaaacc aagtaacttt gaaagaagac
     2281 cgcgttcttg catggttggc taatggagct caaccttcag acacagtacg taacatcctt
     2341 tcaaaagaag gcgtattgaa aaaattccac gattctaaat tttcaaaata agtttaaagt
     2401 aggttgacag atggatacga ttgaaaatct cattattgcg attgtgaaac ccttaatttc
     2461 acaaccagat gccttaacta tcaagattga ggatacacca gaatttttgg aatatcattt
     2521 gaatcttgat caaagcgatg tgggtcgtgt aatcggtcgt aagggtcgca ctatttctgc
     2581 gataagaacg attgtctact ctgtcccaac tgaatacaaa aaagtaagaa tcgttattga
     2641 cgaaaaataa gaagggcggg acggatgtct cgcttttttg tgtggaatag aagatatagt
     2701 aaaatgaaat aataacagga caaatcgatc aggacagtca aatcgatttc taacaatgtt
     2761 ttagaagtaa aggtgtacta ttctagtttc aatatactat aaaagattga acgtttaggc
     2821 aattccaaga ctacttactc gaacattaac agcagtctcg gactgatgaa ggtctcttta
     2881 tgaagttagt ggagaagtcg gagaagtagc cgaggttttg aataggcgct ctggtcgaaa
     2941 agaagggata ggattcgaac gaggaacttg ccagagaact ggctgatatc atttactaca
     3001 ccgtcgcaat cgtagctatt aatcatattg accttaccaa gactgtcttt gagaaagaca
     3061 aaaccgcagc cgttaattac caacataaac atgatttgga aggatttttg aagggaaagt
     3121 agagatgttg tccccttcct ccaacttgag aaaatccttg gaacgtgata aaataaaaga
     3181 taaggatttt taataattca ttatatagaa tgagtggatt tctttatgaa gaggtttgaa
     3241 gtatctacag aaattggtag cctctctgtt acttatcaaa agcaaaagaa agtgctagtt
     3301 tgtctaaacg gagcaggttt gctaccaagt tatgaaaatt tttcacttat acttgaaaaa
     3361 cctcctccta caattggtta tttgacaatt gattttccga acacaggtag gagtccgatt
     3421 catgaccaag ctggaaaaaa tctagataat cttgcagatg tggtttatga agtacttgaa
     3481 gaattaggga tttctgaata tatactttgt gcacatagtt ggagtggaat tttagcttgc
     3541 aaattactcg agaaaccaat taagtgtcag actttagtag caattgaacc gacaactaaa
     3601 aaagtcatgt ttgctgattt ttcagaaaat ccttatccag aaatggaaga gcagatgagg
     3661 ctgattgacg agtgtggtcc tgaactttat tttaagaact taactcaagc aacatttagt
     3721 cctgaaacga ataaaaaaat ctgggaatta atgcaagaaa aaggcttaga gttggaaaat
     3781 caagatccag aatttcagat atctggagag attactgagg aagattttga gaatttgtcg
     3841 atagaatctc atgtccctgt atttattttt tgtcagactt atagagaaaa agagtacaga
     3901 gaatcagaat attggacttc taatactaaa ctcattttag gagggaatca ccattattta
     3961 caatggtcag aatcggaaaa aattgcggct attattcgag aattgtcaga ataagatgga
     4021 aaaaaggaga ttacaggaga caagatgaac tactttaatg ttgggaaaat cgttaatacg
     4081 cagggattac agggtgagat gcgagtcttg tctgtgacgg attttgcaga agaacggttt
     4141 aaaaaaggag ctgagctggc tttgtttgat gaaaaagatc agtttgtcca aacagtgacc
     4201 atcgctagcc accgtaaaca gaagaacttt gacattatta aattcaaaga tatgtaccat
     4261 atcaatacta tcgaaaagta caagggatat agtctcaagg tcgctgagga agatttgaat
     4321 gacctagacg atggtgaatt ttactatcac gagattatcg gtttggaagt ctatgagggt
     4381 gatagcttgg ttggaaccat caaggaaatc ctgcaaccag gtgctaatga tgtctgggtg
     4441 gtcaaacgaa aaggcaaacg tgatttgctt ttaccttata tcccaccagt ggttctcaat
     4501 gttgatattc caaataaacg ggtcgatgtg gaaatcttag aagggttaga cgatgaagat
     4561 tgatatttta accctctttc cagagatgtt ttctccactg gagcactcaa tcgttggaaa
     4621 ggctcgagaa aaagggctct tggatatcca gtatcataat tttcgagaaa atgctgaaaa
     4681 ggcccgtcat gtagatgatg agccctacgg aggcagtcaa ggcatgttgc tcagagcaca
     4741 acctattttc gattcctttg atgctattga aaagaaaaat ccgcgcgtta ttctcctcga
     4801 tcctgctgga aagcagtttg atcaggctta tgctgaagat ttggctcaag aggaagagct
     4861 aatctttatc tgtgggcact atgagggtta tgatgagcgc attaagatct tggtaacaga
     4921 tgagatttcc ctaggcgact atgtcctcac tggtggagaa ttggcagcta tgaccatgat
     4981 tgatgctaca gttcgcctga ttccagaagt gattggcaag gagtctagcc accaagatga
     5041 tagtttttct tcaggtcttt tagaatatcc tcagtacaca cgtccctatg attatcgagg
     5101 catggtcgtg ccagatgtat tgatgagtgg ccaccatgaa aagattcgtc agtggcgatt
     5161 gtacgagagt ttaaagaaaa cctacgagcg cagaccagat ttacttgaac attatcaact
     5221 gacagtagaa gaagaaaaaa tgctggcaga aatcaaagaa aacaaagaat aaaggagaaa
     5281 cctatgcaag taatcaaacg taatggcgaa attgctgaat ttaatccaga taagatttac
     5341 caagccatct tgaaggcagc ccaaactgtt tatgttttga cagatgattt gcgtcaaaat
     5401 cttgctcaag tcactaagaa ggtagttttg gatttacaag aagccaaggt ggaacgtgcg
     5461 actatcagta tgattcaatc tatggttgaa catcgtttat tgggcgcagg ttacattacc
     5521 attgcagaac actacatttc ctatcgttta caacgtgact tggaaagaag tggttatgga
     5581 gatcatattg cagttcattt acattttgaa caaattcgct aagaaaaaag agtgggatga
     5641 agcaactaca tctcactctt tcttaaatct atttttttgg gctgtttgga cagttgaggg
     5701 gacaaatcgc aggcgttgaa tatcgcttat acggataatc cggctcacat ttttggcaaa
     5761 atttttcacg ataatttgct ggccttgctg atcatatttg atgatatcac ctgtaaaact
     5821 tgtctcagac aagataaggt gaacagcggt ttgtttctgg atagcctctt caatagtggc
     5881 tgtaagggag ttggtttcag tctggtcagg ttggtcatgt ccatttaaaa aatcatgtat
     5941 tttatctaga acttgctgga atttatgtct catattggcc tcccttcttt tatatcaata
     6001 ttatataaaa ttttcctaaa atctacaaga ttttacgaat aaactaatga aactaaaaaa
     6061 ggagaagaaa atggcagaat ttacatttga aatcgaagag cacttgttga ctctttctga
     6121 aaacgaaaaa ggttggacca aggaaattaa ccgtgtgagc tttaatggtg cccctgcaaa
     6181 gtttgatatt cgtgcttgga gtccagacca tactaaaatg ggcaaaggga ttactctctc
     6241 aaatgaagaa tttcaaacga tggtggatgc ctttaaaggc aactaatact cttcgaaaat
     6301 ctcttcaaac cgcatcagct ttgccttgcc gtatgtatgg ttactgactt cgtcagtttc
     6361 atccacaacc tcaaaacagt gttttgagca gcctgcggct agcttcctag tttgctcttt
     6421 gattttcatt gagtataagt tttcaaaata aaaaaaagga tattgagtca tgataactcg
     6481 gtatcctttt ttagttagta aagtaagcct gatttttaag gcgttgaagt aggggacggc
     6541 taataaaact aatagccaga agtttgccaa ggtctggaat gataaaggga agaaccccca
     6601 caacaagagc tttttcaaat gccattccag ctaggaaatg caagctgaga atcccgccga
     6661 caaagacaag ggcatcaccc aagaggtttg caagaaaaat cttaacaaca ccactcttgc
     6721 tgttggttag agaggaagta agtccagagt aaacgagata aaaccaaaga tagcctgcag
     6781 tagggccaac taaagcctga aaaccagctc cacctcctgc aaagacagga agaccgatag
     6841 cacctagaag aagatagagt ccagcagaaa gtacagcctc tctcggtcta aagacagtag
     6901 caatcaagcc gattgcaaag ttttgcagag tgaagggaac aggtccaatt ggaagactga
     6961 tttgtgccaa aacagcaatg agagcagccc caatagcagg gatagcataa acgtgagctt
     7021 ttttcaaaat agttaacctc ttttctaatg tatagtaact attatactca tataggagtc
     7081 gatgtcaacc attttttaaa aacaaggtaa caaaaactgt aacagccgat tttcgtgact
     7141 agtcaagttg gataattttt ttgttgattc gaaaattgtc tgtatagata atcctattt
//
LOCUS       DAVKLX010000073         7020 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKLX010000073 DAVKLX010000000
VERSION     DAVKLX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.073"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            108..407
                     /locus_tag="VW325_001964"
                     /pseudo
     CDS             108..407
                     /locus_tag="VW325_001964"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(685..1455)
                     /locus_tag="VW325_001965"
     CDS             complement(685..1455)
                     /locus_tag="VW325_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU9735292.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGYLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(1470..2096)
                     /locus_tag="VW325_001966"
                     /pseudo
     CDS             complement(1470..2096)
                     /locus_tag="VW325_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     gene            complement(2002..2604)
                     /locus_tag="VW325_001967"
     CDS             complement(2002..2604)
                     /locus_tag="VW325_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9735293.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSTGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGRKGGISSSCWGSVVVTSSERLLETSLR"
     gene            complement(2614..2931)
                     /locus_tag="VW325_001968"
     CDS             complement(2614..2931)
                     /locus_tag="VW325_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9735294.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(2948..3265)
                     /locus_tag="VW325_001969"
     CDS             complement(2948..3265)
                     /locus_tag="VW325_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9735295.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(3280..4176)
                     /locus_tag="VW325_001970"
     CDS             complement(3280..4176)
                     /locus_tag="VW325_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU9735296.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(4179..5441)
                     /locus_tag="VW325_001971"
     CDS             complement(4179..5441)
                     /locus_tag="VW325_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9735297.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            5721..6899
                     /locus_tag="VW325_001972"
     CDS             5721..6899
                     /locus_tag="VW325_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9735298.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT         2450 a         1468 c          995 g         2107 t
ORIGIN      
        1 agtttctata gtaaaatgaa ccaaaaatag tacacgttgc ggtataatgt tcttgtggca
       61 tattcattag attttcgtaa aaagttctct cctatttgtg agcgaacagg tatagtcaat
      121 tgattgtcag tttgaagacc aagagtcgta aggcaaagga tatggcagaa tccatccaag
      181 ggtggctggc acaatttcta gtgaacctct ttaaatctat tacgtttgac tgtggaaaag
      241 agttttcaaa atggaaagat atttccaatc atcacgatag tgagagcttt tttgcgaatc
      301 taggatgtcc aagacagcgg tgtctcaacg aacattcaaa tagactttta aggtgtcatg
      361 acttgccaaa gcagacagat ttcaacgaag tcagtcaaga attttaggct gggtaaaaac
      421 tcaatttcag gagaaaatga aataaatatt cccacaataa aacgcatagt atcaaagttt
      481 ttccaacacc tgatattata cattttttaa ttttaaagac tttttcccag cctcaagaaa
      541 tcacatccca cctaaatcct ttggatacca aatacctcat caggtatttt ctgagttggt
      601 aagactgtct agcttaattt gacaaatgga gggcgttaaa tccagttttt acaggaagtc
      661 tatttaaata ttttcacact gaccttacaa aatatgaaat aaattatata aagtcattcc
      721 catgacaaag aataacatcc ctttaaaaca gtatcttacc caattttcag gtaagacttt
      781 tgatacaacc gtccccaata cccctccgca tatagaagca agaaaaataa ctggaacagg
      841 tgctaaatga tactgattta aacctgtaac cacaacaatt gtagccaaat gagacagctg
      901 ggaaaagaaa ataattgcaa gagaatacat cgttgcttct tttatcgaaa tagaaaaaaa
      961 caccatcaat aaagatacat tgagcggacc accaccaatc cctaaaaaac tagaaatcaa
     1021 gcccaataga agaccacaaa tcatatagaa tcctatctta tcaaattgat aagttttttt
     1081 aaaatctgtt aaaacaaatg atacaagtaa catcacaaat agtaaaatca tttgaacaat
     1141 agatactagc cgtactgata attgagataa caaaacttga aaaataagat aacctagaaa
     1201 tcctcctaaa acagaagaaa aggataccgt aaaaatcaat ctccatttta tctctttact
     1261 ttgtgaaaat cttttgattg ttgaaataat cgccattata aaaacagaga aactagaata
     1321 aaaagcaata tcactgaccg aatggtatcc aaaactatcc ataagtggct taattataat
     1381 tccaccaccc atgcctgata tagaacccaa tccactagct attataatga ttagaaaata
     1441 aataatctca accacttttt aagtccatcc tattctttca aacatctaaa acctagatgg
     1501 ctactagtgg aagtagaaat acatccattt ctagcagcta ctcggtatcg attacaataa
     1561 gaacaatgac ataaaaagga acctcctctg atagcatact cctcatcttg tatcccatat
     1621 tttggtaact catagtcatc ttcattaaaa ctagctaata aggtatatct cggattgcga
     1681 caccattccc aaacattccc aatcatttga tatagaccaa aatcattagg taaaaattca
     1741 tagacaggag ccgttcctat aaatccatct aaggcagtat tttcataagg aaatcttcct
     1801 tgccaagtat tggcatgata atatccacct tctaaaagag tatctcccca aagatattca
     1861 gattgtcttc ctcctctagc agcatattcc cactgtgctt cagtcggtaa agacattccg
     1921 ctccaattac agaaagccaa tgcatcttct aaagcaacat gaactactgg atggtcttct
     1981 aaacccacta gattactgtt ctcaccgtag ggatgtttcc agcaagcgtt cggaacttgt
     2041 aaccaccacg gagccccagc aggatgagga tatccctccc tttctgcctc tggcattaat
     2101 atggtttcct ggaatcacat tcccaatagt taccatcaga tttttaccat caaatgctag
     2161 attatcaatt ccttgtaaga tctgatctaa tttcacatct cctacatttg ccaaagggaa
     2221 actctttgta actgtacttc caatcgaaat gacaaacgaa gcaagtgata ccaaaccagt
     2281 agaaactgta tcaaccttgt aaatcttagc gatattcact cccaagcaat agatgaacaa
     2341 caaggaaaca attggtatac ttcccttgaa taccaaatta ttgatgtcaa caagccactg
     2401 aaaggttttc gtaatacttt ctaggtgaaa ttgttgtggt aaatccacta gaaaagcatt
     2461 taataacaaa gcaatggaac ctgtcataat aacaggcata gtccccacaa atgaatcacg
     2521 taaagcaact aaaaacttct gattcccaac tttcattgca attggagaaa gtttttcttg
     2581 cattttatcc aacattgtat tcataccaaa ctcctaattt ttttcaaatc gttcgtatac
     2641 ttctaccact tctttcgcca agtcaacaaa agtcagactc gtcattaaat gatcttgcgc
     2701 gtgtaccatc aagaggctaa ttgatgtttt ttccccctgt gcctctcttg tcaacatttc
     2761 tgtttgagcc ttatgcgcct gtaataaggc agcattcgca tcagctaatc ttcgattggc
     2821 tttagagaaa tccccttttt ttgctgcctg aatagcctcc atagcattac ttttggcttc
     2881 cccaccatac ataattagcc ccattacaga ttctaaatta ctttcatcca taacattctc
     2941 ctcaatctta gtctaataaa ctttcagcta aatctaaaac tttagaacca ttcatcattc
     3001 cgtaatctgt catcggaata accgccacag gaatctgtct atcttttagt ttttcttgaa
     3061 agtcccctag taaatagcga acttgaggac ctaaaagcaa cacatttact tcttttgttg
     3121 ctacaatttc ttctgcttca ggagctggaa ctgcaaaaat ttctgcatcc aaccccttat
     3181 cttctgctgc cttttgcatc tttgtcacta gcagacttgt actcatacct gctgcacatg
     3241 ctaacataat tgttacttta gccatagctt actcctttat cacctattta ctgacctagc
     3301 caagcgagat actgtatgtc tcactcctct aattgccttg ctcaatgcat aaatattatc
     3361 gactgttgcc agtcccccat atcctgcgtc acctatatgt tgaatatcaa ctccacaaat
     3421 tttatttcta agtgcaattt ccttgatagt atctgtatca gatgtttctt ggctagtacc
     3481 aatagcactc aatacaagac cacccttact atgaacgaga tcaacgactt cacgcaactc
     3541 ttggtcatga aaggctggaa cggttccaac tgctggaaca agtatcacat ccgcacctgc
     3601 ttccaacaat tgctctgcga cagaaagctc tgccacaggc tcattcactc ctgccccgtg
     3661 cattttacca gctataatca aacctgagaa attctcttta gcagattgaa ctgctttgat
     3721 aatctcttga ttgctaactc ctgttccagg atttccagtc aaacagacaa agtcaaaacc
     3781 taactcctct attcgattca atgtttcaac actagcaaca cgacctgcaa caatttcctg
     3841 tgtttcctct agcatctttg cagatgggtc aatcggttcc aaatttacac caattggaca
     3901 agcaacaagc tggtgtaagc gatgaataac atcatcacct gaactatcca aagcataaat
     3961 tttaggctca aaaacatcaa cacaattcaa taaaatcata tcagctccaa aagctctagc
     4021 aatttcagaa ttagttatat ccccaacaaa tgtctcacga gtaactacat tttcagatag
     4081 aacaactcta ccttcacttg ctaaaatact ttgttttaat tcttcagcat tcatctgtaa
     4141 tatctcagat ggatttgcac taattaaacg tttcattctc aatcctccaa taacttaaac
     4201 cgtttttcaa tagctattac aaatggtaaa taaagtaaga catccaaaac aatcaataca
     4261 atttgtaata atgaaccaga aatacttcct gtagcaagta aaccacttat aacaggtggc
     4321 atagtccaac ctggatctgt ataagttagc ggaaccaaac ctgatgccat tgctccccac
     4381 gctactagta agttaaacat tggagctaag ataaatggaa ctagtaacaa aatgttcaaa
     4441 acaataggaa cgccaaacat tgctggttca tttatattaa ataatccagg tataacagtt
     4501 ataggtgcca aagtttttag ttgtttacta gctttcttct tatgtgctaa atatcctaac
     4561 gcaagaacta aacctatggt tgcaccaccg ccacctatga atacaaaatt atccataaat
     4621 acgctagtaa aaatatttgg aagagtttct ccagtttgat aagctacttt atttgcatct
     4681 agattagcta accataacgg tttcataact gcattaacaa catttcctcc gtgtaatcct
     4741 acaaaccaaa aactactatt aaggaaaatt aagataagaa gaccaataac attatttcct
     4801 aaaagtccaa gtggacctcc caaagctact tgagcaattt catgaagatt aggtaaactt
     4861 aatttatcta aagttgcata aacaatcaac caaccaacaa tagtcactgc accaggaata
     4921 atggcactaa agcttttaga tactgctggt ggaacaccat ccggcatttt tatctgtata
     4981 ttatgattta taaaccattg gtagatatag ccattaatca atcctaaaat catagcaaca
     5041 aataaacctt ttgatgccat gaaagcggta ggcattcctg ctccagcctc tcctctaatg
     5101 aaaggcgtaa ctgtaataaa agatgataat gacacaatac cagcaggaat accatcaact
     5161 ttgtattgtc tagccataaa gtaagcgata ccaaaactag ctacaagacc aagtaatcca
     5221 aaactactat caacaccctt ccataaatag tcagcaacac caatatcacc cagatatttt
     5281 tcccatcctg gaataggaaa acttgcaata accattagta atgaacctat caacaataaa
     5341 ggtatagtta gagtaatacc gtctcgaata gaaaccaacg ctttattatt acccaatcta
     5401 gccgctactg gaagtaactt ttcttgtaat aatttttcca taaccttttc ctcttaaata
     5461 tattttatta tcaaaagtta tcaattatct ttattttttg acgtcaagtt gttatttgat
     5521 tatcttttgt tatttatatt ttacaactat aagaaagcct tgtcaaggat ttgtgtaaac
     5581 atttgattat tttttattat caatatataa taaaaagaga ccagtaagta atgaaaagct
     5641 taaaaaaatt aacatctaca tttatccata gcaaaaaatc ataggatttt aagaatctga
     5701 caactaggaa gaaaactaat atgaccttat ctaagaaaca attacaacta agagctaaaa
     5761 tactggaaac agtttacaca ttaggtccca tttcacgaat tgaaatcgct actaaaacag
     5821 gaataacacc tgcaacaact agcagtatca caaatgatct gattaaagaa aatattctct
     5881 tagaacttgg tgaagacgaa cacgatacaa gcgtagggag aaaaaaaatc ctgctagaca
     5941 ttcaagctaa acgattttac tacattggtt gtgaactatc tgaaaaacat ttcacttttg
     6001 ctttaggaga taatctagga aatatcttaa aggaagagaa agaaattgtt actaaacagt
     6061 taatccaaga aaagggaaat caactcatta accagacact aaagcaattc ttaaataact
     6121 gctctgatta tgaaatagaa gcaattggaa tcgcgctacc tggtcgctat ttagataatt
     6181 ataaaataac aacaaacaat cctttatggc aacatatcga cttagaaatg attcaatccc
     6241 actttgataa gcctttgttt ttctctaaca atgttaactg tatggctata ggtaaacgcc
     6301 tatttagtcg ccaacagaat gaccctaatt ttgcttattt tcattttgct agaggtatgc
     6361 actgttctta tatctacgac ggaaacattt atgggaaagg aaatctaatg attggagaaa
     6421 tagggcatac agttgtttca tctgagggag aagaatgtag ctgcgggcgc aagggatgct
     6481 tacaaacttt tgctggagaa tcttggttaa tcaaaaaatc aaaaattcta tatcatcaat
     6541 ctccctattc tctacttcct agtttagtca aaaatgccga tgatattgat atccaagtta
     6601 ttttaaccgc ttatcaatta ggtgacactg gaataatcac acttatccat caagccttac
     6661 tctatcttag tcaaaccatt ttaaatatca gtatgatgat tgactctcaa aaaatctacc
     6721 tacatagtcc tttactaaca aatcaacaca tcatccaaaa actatatagt gaaatgaact
     6781 acaaacccaa actgctctat aatcgcctac ctgaagtcat tattgaacct tacaatgatt
     6841 ttacagccgc acactcagct attgcactct gtttgtatca tacaattcta cattcttaat
     6901 tttctattac atttaataaa taggctaaaa agagggagta tatcataaaa agcgaataaa
     6961 cctgttttct gaaaatcaga attctgtttt gttcgctttc tttatatttg gaattatact
//
LOCUS       DAVKLX010000074         7013 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKLX010000074 DAVKLX010000000
VERSION     DAVKLX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.074"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(102..377)
                     /locus_tag="VW325_001973"
     CDS             complement(102..377)
                     /locus_tag="VW325_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9735299.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(484..1323)
                     /locus_tag="VW325_001974"
     CDS             complement(484..1323)
                     /locus_tag="VW325_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9735300.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1464..2312
                     /locus_tag="VW325_001975"
     CDS             1464..2312
                     /locus_tag="VW325_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9735301.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2405..3322)
                     /locus_tag="VW325_001976"
     CDS             complement(2405..3322)
                     /locus_tag="VW325_001976"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9735302.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3760..3965
                     /locus_tag="VW325_001977"
                     /pseudo
     CDS             3760..3965
                     /locus_tag="VW325_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4276..4569)
                     /gene="rpmA"
                     /locus_tag="VW325_001978"
     CDS             complement(4276..4569)
                     /gene="rpmA"
                     /locus_tag="VW325_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9735303.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4586..4930)
                     /locus_tag="VW325_001979"
     CDS             complement(4586..4930)
                     /locus_tag="VW325_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9735304.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4946..5260)
                     /gene="rplU"
                     /locus_tag="VW325_001980"
     CDS             complement(4946..5260)
                     /gene="rplU"
                     /locus_tag="VW325_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9735305.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5296..5383)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5442..6146)
                     /locus_tag="VW325_001981"
     CDS             complement(5442..6146)
                     /locus_tag="VW325_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9735306.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     gene            6549..6848
                     /locus_tag="VW325_001982"
     CDS             6549..6848
                     /locus_tag="VW325_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9735307.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2217 a         1420 c         1167 g         2209 t
ORIGIN      
        1 gaacattcaa ctttttaact ctatttatta ctagatttca taattaaaaa gcctactgac
       61 caagctagaa agcttgatac aataggcttt ttaaagactg attatttaac agcgtcttta
      121 agagctttac cagctttgaa tgctggtact ttagaagctg caattgtcat ttctttacca
      181 gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa cttcaaagtt accaaaaccg
      241 atcaactgaa ctttttcacc agctgcaaga tagtcagcta ctgctgcaaa tacagcttca
      301 actgctgctg ctgagtcttt cttagtcaat tctgtagctt ctgctacttt agcgatcaaa
      361 tcttgtttgt ttgccatgtt aatgattcct ccaaataatt tctaattaac aaatataatc
      421 atatcctaaa atccctttta ggtcaagtta aaaacgctat ttcttcccat tttatttatt
      481 tttttaggag tggtaacgta tcaaaatagc ccaagcgttc tcacccgtgt gagtttgaat
      541 aatggaaccc gtttccaaaa cagaaattgg cttttcaaca taagcttgta agctttcttt
      601 catctctttt gcccaatcat cactaccaga atatgaaatt ccaatctctg ctacagcacg
      661 ttcagaaagc gatgttatca actcatctaa ccatttttta aatgttttag ttccacgacc
      721 tttaaccatt ggctgcaatt catggtcttt catttgcatg acaacacgga tattgagaag
      781 agagctcaac aagccagtta cacggctaat tcgtccacct ttgacaagat tttccaaagt
      841 tgaaacacca atataaagct ctgtatggtt tttaacctct tctacatgag ataaaattgc
      901 ctccatatct ttaccttctt gagctaactt cgcagcctca acaacttgga atttcagggc
      961 ttggtcagtg aaggaactat caacaacagt cacatctgca gtagataggc tagcaccttg
     1021 gcgtgctgct tctaccgtac ccgaaagagc atgggacata tgaatagcaa gaatctggcc
     1081 accatctttg cataggtctt caaaaatctc agcaaagaca cctacaggtg gctgacttgt
     1141 tttcggaaga ttcttacttt cttgcatcaa ctgaagaaat ttaccttctt ctttcaaatc
     1201 cgcatcagaa taaacaacat tatcaatcat tacagataat ggaacaattg taatatctaa
     1261 ttgctttact agttcaggtt caatagtaac agatgaatcg gttacaatct taatttttgt
     1321 catagtatca atctttctat tttaggattc agattggttt ccttacttct aattatatca
     1381 aaaaaagatt aaaaatccta atggagtcaa tcaaattttc cgtaaaattt gatataatca
     1441 acttataaga aaagaggtgt cctatgatta aaaaaattta ccccattttt accattttac
     1501 taggtgctgc tatttatgct tttggactga cttattttgt agttccccat catctctttg
     1561 aaggaggggc gacaggcatt accctcatca ccttttatct ttttaaaatc cctgtttccc
     1621 tcatgaacct gctgattaat attccccttt tcatcctagc ttggaagatc tttggagcca
     1681 aatccctcta ttctagttta ctaggaacct tagctttgtc cgcctggtta gctttttttg
     1741 agcatattcc ccttcatatt gatcttcaag gtgatttact aatcacagcc cttatagcgg
     1801 gaatcctatt gggaattggc cttggaatta tttttaatgc tggaggtaca actggcggaa
     1861 ctgatattct agctcgtatt ctcaacaaat acactcatat atccatagga aaactgctct
     1921 ttatcttaga tttttgtatt ctcatgttga ttctcctaat cttcaaggat ttgagattgg
     1981 tttcctacac gcttttgttt gattttattg tttctcgtgt tattgatttg attggtgaag
     2041 gaggatatgc cggcaaaggc tttatgatta tcacaaaacg tcctgaccaa cttgctaagg
     2101 cgattaatga tgacctcgga agaggtgtta cttttatttc tggtcaaggc tactatagta
     2161 aagaaaattt gaaaatcatc tactgtattg tcggaagaaa tgaaattgtg aaaacgaagg
     2221 aaatgattca tcgaatcgat cctcaagcct ttataactat tacagaagcc catgaaatcc
     2281 taggagaagg cttcaccttt gaaaaagaat aaaaagaggt aatgtcgtga cctcaaaagt
     2341 tagactaaat catctatctt ttgggttaca gacaacctct tttttgtttt atttactcaa
     2401 gctcttaaga ccaattccga gttacttctt catcagcctt taactgatcc actaattggt
     2461 caactgagtc aaatttggtc atatcacgaa tgcgatcaag ccaataaacc atgacggttt
     2521 ccccataaat atcttgatta aaatcaaaaa tattgacttc aaaacgtgct tcttctccat
     2581 caaaggtcac atttttcccg acactagcca tagcacgata cttctgtctt tgaatctcaa
     2641 catcaacgac ataaacgcca tctgctggca tataagtacg gtctaaaagc actaaattcg
     2701 ctgtcggata accaattgta cgaccacgag cattaccatg aaccaccata cctcttgatg
     2761 gaagcggtgc ccccaaaagt tttcctgctt ctttcacatt tccatctaaa atagcttgac
     2821 ggatacgagt tgaactaatc tttcctttct catcttctac aggtggaaca atgataactt
     2881 ctccatcaaa gtaatccttt aaatcttctg ctgttttttt gtcagaacca aatgtataat
     2941 caaaacctgc aacaataatt ttggcattca tagccttgat ataagttgca aagaattctt
     3001 gtgcagtgag actagcgaat tgactactaa aatcaaggag atataattct tctacacctt
     3061 cgcgctttaa ttttctttca cgttcagcag ggttcaaaat atgcaaaaac aaatctggat
     3121 gataaggctc taaagcgatc tttggagatt cattaaaggt cataacgacg ataggtaata
     3181 aatcctttct cgcagccttg ttggcaacac gaaataattc ttgatgcccc ttatgtatgc
     3241 catcaaaata gccgagaaca acgactgaat cagatggtgt gccaatatct ttttggtttt
     3301 ttataggaat agtaataatc ataaaataat tatatcatag cgatagctat ttctggaaca
     3361 gaaaatctga aatgttgttt ttttcacatg aagtgtacct gttttcaaaa agcactttat
     3421 tctatcgttg cttaactatg aactttgcaa tattcttctc aaaaacttgt aggacatctt
     3481 caaaattttg caaggagtga ttagacttgt tcggtaacca taaagtgtca tactatgctt
     3541 atgtatgaaa aagcaatgca actaactcct gagaacttta aattactaat tggtgccgaa
     3601 aaggtagaat ttagaatcga ggtacaccta tggctgtaaa atttacaaaa tgagacaact
     3661 tgggcaagat gtttgaagaa tttcctaaac tccctgattt gaagcaagtc actttccctg
     3721 atgacaaaga aaaaagccaa aacagcaaag aaaaactaga tgactgcttt ccaacaactc
     3781 ccatctagtg tgcttcagac taggctattt ttctctccat ctgttagctt ggattctcag
     3841 accgtttcag ctaaagaata tcttttccct tatcagaagg aacggctcaa accattcaga
     3901 caagtgaagg gacgacaagc caatatttga aaccagatag cagttcttat agtcaattga
     3961 aataaaatct gaagaaatcg agtaggaaac tcatatcaat ttttaacagt gttctattcc
     4021 agattcatac tcaatgaaaa ttaaagtgca aactaggaag ttagccgcag gctgtacttg
     4081 agtacggcaa ggtgaaattg acgtggtttg aatttgattt tcgaagagta tcaatccact
     4141 atacatatta aaaagtatgg gtatcaaaat aaaaaaacag gctctccgaa aactcggaaa
     4201 gccttatttt aagctgtttc tagcttcctc acctttacat ttcaaggttc gggataaaag
     4261 gttcaatgaa cctttttatt ttgcgattgg gtaaacagaa acttgttttt tatcgcgtcc
     4321 tttacgttca aagcgtacta cgccttcaac tttagcgaac aaagtatcgt ctccaccacg
     4381 accaacgttt acacctggat agatgtgtgt accacgttga cggtaaagga ttgatccacc
     4441 tgttacagtt tgtccgtcag ctgctttagc tccaagacgt tttgcttgtg aatcacgtcc
     4501 gtttgatgta gaacctccac cttttttgtg ggcgaaaagt tgcaagttgt taagagtcat
     4561 ttttaacata atgttttcct ccgtgttagt tttctgtgat aactctggtt tggacgaact
     4621 cagaagagtt ctccgataag tttgccatac ctaagaaaaa tgattcaaag aataactggg
     4681 tcatttctct ctggtgtgaa ggaagatctt ttggtatttc aaccatcaga tagccacctt
     4741 catcttcgtt taattctagg attggttcat agcctgcaaa tttctcaata gaattgataa
     4801 agttaatggc aagcgtagaa accgatgcac acacgacatc taagccgtat tcgccactct
     4861 cggcgtgtcc agtaatttcc gcactcctca gctcgccatc ttcggctctc tcaaagactg
     4921 cctgtatcat gtgttctcct taaaattaag cgttgattgc gttgatgaca acttttgtat
     4981 atggttgacg gtgaccttgt ttacggtggc tacctttttt aggtttgtac ttgtaagtaa
     5041 ccactttctt ttgttttcct tgtttttcaa cagttccaac tacagtagct ccagcaacaa
     5101 gtggagttcc gacaacagtg ttttcaccac caacaagaac aacttcgtta aaagtaactt
     5161 cttgaccagc ttcaacgttc aatttttcaa cgtaaactgc ttgaccaact tcaactttaa
     5221 cttgttttcc gccagttttg ataattgcgt atgtgctcat tatgcacctc ctatgatttt
     5281 tatgggtttc cccgattttt tgtgaagact cgcctagcat cgtgggacga accacttaaa
     5341 cttgaataat caagcaacga tgttcgtgcg gttgcacagg atcgtgcata gtcaactctt
     5401 caagtatagc atatctccta ttttcttaca agtaataaca cctaaaatga agctttttct
     5461 tttacttttt tctgccaaga ggcaaaaagc atgctgaggt aaaaaacgct catcataata
     5521 ggaacaccaa gaatggtctt ttcatgatag aaaatcgtca aataggctga aaagacaacg
     5581 ccaaggacaa aactactaag caggctaaca aatatgaatc cttcacgcaa aaaaggagtg
     5641 tgcttggttc ggaaataatc tccaaaagcc agcatggtcc gtttgatatt ccctgtcata
     5701 aaagcgttat tataggcaat acccgacact tctccaaaag cagttgtcac cagtcccata
     5761 cagaaggcca agggcggcac tagatagata ttatccacag tttgcggcac aaaagcaata
     5821 atgattgata agattgccaa gggaatcaag gacagaatag gttttttcac aattctcaat
     5881 ttttccttat aaatcgttaa taaaaagact cccatcataa acgctagcaa ggtgagaacc
     5941 ttgtccctaa catccgaaac attattttta attaattcta ctgaaagaaa gacaacattt
     6001 ccagtttgtc cagctacaag ggtattcccg cgaacaataa aagtgtaagc atccacatat
     6061 ccagcacaaa acgtcaaaaa aagtgctaac cttttagact gacgtgatat ttttcttata
     6121 ggtaataacc tcattttacc tcccattgta ttttctctta gaaatattgt accattttct
     6181 ttctaaaaaa tcgtaggcta ccatttagat tttactatta gcataaaaat aataatagac
     6241 aactatttat ccaaaaatag atagatgtaa catgtttgca aacaaagcat acgaaccttt
     6301 agtaaaatca tttccatgaa actagaatag agccctctta gcaaaaatca ttattttaat
     6361 ttatttctaa tcactccttg acataaataa ctctcaccaa taaaagacta tgtcttaaaa
     6421 aaaatggtat aataaaatca atacttgggc ttgatggcta tgctactaat aacaattagg
     6481 agagaaaatc aggcacttgt taacaacaag gattatcccc ttgagatgaa aggaacttta
     6541 gaaatcttat gatgaacatg caaaacatga tgcgtcaagc acaaaaactt caaaaacaaa
     6601 tggaacaaag ccaagctgaa cttgctgcta tgcaatttgt tggcaaatct gctcaagatc
     6661 ttgtccaagc gaccttaact ggcgataaga aagttgtcag cattgatttc aatccagctg
     6721 tcgttgaccc agaggacctt gagactcttt ctgatatgac cgttcaagcc atcaactctg
     6781 ctcttgaaca aatcgatgaa actaccaaga aaaaactggg tgctttcgct gggaaattac
     6841 ctttctaaaa acaaggagct agaacaatgc ttgtcgataa caaaggctaa gaaaggtgca
     6901 aaaatgactc tataatattt gtagtgggta aatccactat agatattatg gagcctattt
     6961 tattgtagaa aaaaagtccc ataagatcta taatgaaaag cgaccaaaca act
//
LOCUS       DAVKLX010000075         6800 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKLX010000075 DAVKLX010000000
VERSION     DAVKLX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.075"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..379
                     /locus_tag="VW325_001983"
     CDS             <1..379
                     /locus_tag="VW325_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9735308.1"
                     /translation="RGKVSGRRYQRISLVAGLTNGELIAPMTYEEMMTSDFFEAWFQK
                     FLLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKK
                     HLKKVLPSCNTFYEALLSCSCFN"
     gene            465..1415
                     /locus_tag="VW325_001984"
     CDS             465..1415
                     /locus_tag="VW325_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9735309.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYDDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPISLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1592..2599
                     /gene="gap"
                     /locus_tag="VW325_001985"
     CDS             1592..2599
                     /gene="gap"
                     /locus_tag="VW325_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9735310.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2666..3502)
                     /locus_tag="VW325_001986"
     CDS             complement(2666..3502)
                     /locus_tag="VW325_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9735311.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3628..5823
                     /locus_tag="VW325_001987"
     CDS             3628..5823
                     /locus_tag="VW325_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9735312.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQSLAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5876..6028
                     /gene="rpmG"
                     /locus_tag="VW325_001988"
     CDS             5876..6028
                     /gene="rpmG"
                     /locus_tag="VW325_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9735313.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6038..6214
                     /gene="secE"
                     /locus_tag="VW325_001989"
     CDS             6038..6214
                     /gene="secE"
                     /locus_tag="VW325_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9735314.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6269..>6800
                     /gene="nusG"
                     /locus_tag="VW325_001990"
     CDS             6269..>6800
                     /gene="nusG"
                     /locus_tag="VW325_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9735315.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         2118 a         1187 c         1469 g         2026 t
ORIGIN      
        1 aagaggtaaa gtatctggaa gaagatatca gaggatttct ttggttgcag gtctaacaaa
       61 tggtgagtta atcgctccaa tgacttacga agagatgatg acgagcgact tttttgaagc
      121 ttggtttcag aagtttctct taccaacatt aaccacacca tcggttatta ttatggataa
      181 tgcaagattc catagaatgg gtaagttaga acttttatgc gaggagtttg ggcataaact
      241 tttacctctt cctccctact cacctgagta caatcctatt gagaaaacat gggctcatat
      301 caaaaagcac ctcaaaaagg tattaccaag ttgcaatacc ttttatgagg ctcttttgtc
      361 ctgctcttgt ttcaattgac tatagttcac ggatacagtt gggaaagaag ctattaaata
      421 tttggagagt ccttttggga tgaacatgtc aaatattact atagatgggc aagaaaagat
      481 gggcaagaaa tggatctgag tcaaacgatg cttcttatgc tcaagtagtg agtctttatg
      541 atgatacaag tatctctgtt agtaataatg agacggacaa agtgttgact ggatccctct
      601 atactgaaac aaatgaacag ggattaacga ttccaatcag tttactaaaa aattggaatg
      661 aacagacagg aaaaaatttg acaactaatg atcttattgg caaatcagtc tcagccagca
      721 ttgtagaaag tgctgccgaa actagtaaga ttgctcaatt tcaaacgaag attgtacgtg
      781 taatcaatga tgaagatgac atggaggaca gcaacagttt catgctgtct catcaaatgg
      841 aaacgatttt gaaagaggct ggatttacga aagctgtatc ttattttatc ttggaactca
      901 aagatccatc acagacaaaa gtagtaacag aagaattaca gaaaaataag aagtatactg
      961 tgctttctca acagagagtt cttgatattg tgattacctt tattcgtgtt attcagggat
     1021 tattgattgt gctttcatca caagctattg tggtagcagc ggttatgatt ggtatcatta
     1081 tttacatcaa tatcatgcaa cgttccaagg aaataggtgt catgaaagca gttggttatc
     1141 agaatcgtgg tgtcaaagga atttttattt acgaggctat ctggattgta ggcatcgcct
     1201 tgctgctggc atttttggtt gcacaagggg tgggaagttt ggcgaatgcg attgtaagtc
     1261 acttttaccc atccatcact aaggtttttg aattaaatct tttatctgtt ttaggaactc
     1321 tagttttcgc tctattactt ggttatgtct cagcctactt cccggcgcgt aagattagta
     1381 aaatggatcc tgtagaatcg ttacgctatg aataataagg gattccttga tttacagtct
     1441 taaaaagaaa ctcacataga aaaacaatgg ccatacttgc aattcaactc aaatagttgt
     1501 ataataggtt tgttgaaagg tgatttgtag tgattcaagt tactcttttc acaataaaaa
     1561 tttcatgatt ttcataagga ggaaatcact aatggtagtt aaagttggta ttaacggttt
     1621 cggacgtatc ggtcgtcttg ctttccgtcg tatccaaaac gtagaaggtg ttgaagttac
     1681 acgcatcaac gaccttacag atccagttat gcttgcacac ttgttgaaat acgacacaac
     1741 tcaaggtcgt ttcgacggta ctgttgaagt taaagaaggt ggattcgaag ttaacggtaa
     1801 attcgttaaa gtttctgctg aacgtgatcc agagcaaatc gactgggcta ctgacggtgt
     1861 agaaatcgtt cttgaagcta ctggtttctt tgctaagaaa gaagcagctg aaaaacacct
     1921 taaaggtgga gctaaaaaag ttgttatcac tgctcctggt ggaaacgacg ttaaaacagt
     1981 tgtattcaac actaaccacg acgttcttga cggtactgaa acagttatct caggtgcttc
     2041 atgtactaca aactgcttgg ctccaatggc taaagctctt caagataact ttggtgttgt
     2101 tgaaggattg atgactacta tccacgctta cactggtgac caaatgatcc ttgacggacc
     2161 acaccgtggt ggtgaccttc gccgtgctcg cgctggtgct gcaaacatcg ttcctaactc
     2221 aactggtgct gctaaagcta tcggtcttgt aatcccagaa ttgaacggta aacttgacgg
     2281 atctgcacaa cgcgttccaa ctccaactgg atcagttact gaattggtag cagttcttga
     2341 aaagaacgtt actgttgatg aagtgaacgc agctatgaaa gcagcttcaa acgaatcata
     2401 cggttacaca gaagatccaa tcgtatcttc agatatcgta ggtatgtctt acggttcatt
     2461 gtttgacgca actcaaacta aagttcttga cgttgacggt aaacaattgg ttaaagttgt
     2521 atcatggtac gacaacgaaa tgtcatacac tgcacaactt gttcgtactc ttgaatactt
     2581 cgcaaaaatt gctaaataat tcttgagttg atagaaagca aggctttgtg gtcttgcttt
     2641 ttatatagaa aaatggatga cacgatcatc cattcttttt taattctttt tcaaatgtat
     2701 ttgaaagggt agtgaaagtt agcttttcta aagtaagtgg gtgggtaaag gaaagtcgga
     2761 aggcatgaag cataagccga cttgtctttg atttactatt atagagaggg tcgcccagga
     2821 taggaagatt atgatgcgaa aggtgcacac gaatctgatg ggttcgccct gtctttagct
     2881 tgcaacgagc caaggaagtc ttgtttgaga attgctttaa tctgcttaca tacgtttcag
     2941 catattgccc attttttgca tcaactattc tttttctacg atcatggcga tcacgtccaa
     3001 ttttgtctct gaaaacaagt tcttttctgt tgatatttcc atcaactaga gcccaatatt
     3061 ctctagaaat ctcttttttc tccaataagc gattgagaat gggcaggata aaaggatttt
     3121 tggcaaagag aactaagcca ctggtttcca tgtccagacg atgaacgaca tagcaggttt
     3181 ggccaacata ggtactgaca tggttaagaa gggcaatttc gtttggttga ttaccatgcg
     3241 ttttcatccc ctctggtttg tttacaataa tcaagtgttg atcttgataa acttcctgca
     3301 ctaagtctgg gttgccccaa gggatcgtct tttggggata atcttcctcg tcaaaagtca
     3361 actggcaaac atctccagga tttacgattt cgttccagtg gacttcttct tgatttatca
     3421 aaatatgttt cttgattctc aaaaaatgac ggatttttct agggatgagg agttgttcct
     3481 caagtaattg ctttaccgtc atttgaggta tagaggcggg taatgtaaat gtgaattgca
     3541 tacagatatt gtaacaaaaa aagccctatt tggataggaa atagctaaat tcttgtcttc
     3601 ctatgatgaa gatgataaaa taaacgcatg aaattagata aattatttga gaaatttctt
     3661 tctcttttta aaaaagaaac aagtgaacta gaggactctg attctactat cttacgtcgc
     3721 tctcgtagtg atcgaaaaaa attagcccaa gtaggtccga ttcgaaaatt ctggcgtcgt
     3781 tatcatctaa caaagattat ccttatacta ggtttgagtg caggcttgct agttggaatc
     3841 tatttgtttg ctgtagccaa gtcgaccaat gtcaatgatt tgcaaaatgc cttgaaaact
     3901 cggactctta tttttgaccg tgaagaaaaa gaggctggtg ccttgtctgg tcaaaaggga
     3961 acctatgttg agctgactga catcagtaaa aacttgcaga atgctgttat tgcgacagaa
     4021 gaccgttctt tctataaaaa tgacgggatt aactatggcc gtttcttctt ggctattgtc
     4081 actgctggac gttcaggtgg tggctctacc attacccaac agctggctaa aaacgcctat
     4141 ttatcgcagg atcaaactgt tgagagaaaa gcgaaagaat ttttccttgc cttagaatta
     4201 agcaaaaaat atagtaagga gcaaattcta accatgtacc ttaacaacgc ttattttgga
     4261 aatggtgtgt ggggtgtaga agatgcgagt aagaaatact ttggagtttc tgcatcagaa
     4321 gtgagtctgg atcaagctgc gactctggca gggatgctca aggggccgga actgtataat
     4381 cccttgaatt ccgtagaaga ttctactaat cggcgcgata ctgtcttgca gaatatggtt
     4441 gcagcaggat atattgataa aaaccaagaa accgaagctg ctgaagttga tatgacttcg
     4501 caattgcacg ataagtatga aggaaaaatc tcagattacc gttatccctc ttattttgat
     4561 gcggtggtta atgaagctgt ttccaagtat aatctaacag aggaagagat tgtcaataat
     4621 ggctaccgca tttacacaga gctggaccaa aactaccaag caaatatgca gattgtttat
     4681 gaaaacacat cgctatttcc gagggcagag gatggaacgt ttgctcaatc aggaagtgta
     4741 gctctcgaac cgaaaacagg gggagttcgt ggagttgtcg gtcaagttgc tgacaatgat
     4801 aaaactggat tccggaattt caactatgca acccaatcaa agcgtagtcc tggttctaca
     4861 attaagcctt tagttgttta tacaccagca gttgaagcag gctgggcttt gaataagcag
     4921 ttggataacc ataccatgca gtatgatagc tataaggttg ataactatgc agggatcaaa
     4981 acaagtcgag aagttcctat gtatcaatcc ttggcagaat cgcttaatct acctgctgtt
     5041 gccactgtta atgatttggg tgttgacaag gcttttgagg caggcgaaaa attcggactc
     5101 aacatggaaa aggtcgaccg tgttcttggt gtcgccttgg gaagcggtgt tgaaaccaac
     5161 cctcttcaaa tggctcaagc atacgctgcc tttgcaaatg aaggtttaat gcctgaagct
     5221 cattttatta gtagaattga aaatgctagt ggacaagtta ttgcgagtca taaaaattca
     5281 caaaaacggg tgattgataa gtctgtagct gacaagatga ccagtatgat gttggggact
     5341 ttcaccaacg gtaccggtat tagttcatcg cctgcagact atgtcatggc agggaaaact
     5401 ggaacaactg aagcagtttt caatccggag tacacaagtg accagtgggt aattggttat
     5461 actccggatg tagtgattag ccactggctt ggctttccga ccactgatga aaatcactat
     5521 ctagctggct ctacttcaaa cggtgcagct catgtcttta gaaacattgc caataccatt
     5581 ttaccttata cgccaggaag tacctttacg gttgaaaatg cttataagca aaatggaatt
     5641 gcaccagcca atacaaaaag acaagtacaa accaatgata atagccagac agatgataat
     5701 ttgtctgata ttcgagggcg tgcgcaaagt ctagtagatg aggctagccg ggctatctca
     5761 gatgcgaaga ttaaggaaaa ggctcaaaca atatgggatt cgatagtcaa tctatttcgc
     5821 taagatgctt gtcaaagcct agctttcttg ttataatgga taagatggag gcgttatggc
     5881 actaaaaaaa gcaagcctag cttgtgcggt ttgtggttcg agaaactatt caatcaagat
     5941 cagcggaaac cccaagccta cacgactaga agtaaataaa ttttgtaagc attgtggcaa
     6001 gtacactaca cacagagaaa cgagatagga gagagcgatg cgttttattg gagatatttt
     6061 tagacttctt aaagacacaa catggccaac tcgcaaggaa agctggagag attttcgttc
     6121 tatcatggaa tacacagctt tctttgtagt aattatttac atttttgacc agttgattgt
     6181 ttcaggtttg attcgattta ttaacatttt ttagaagatt agtggagtta attacactag
     6241 aaatcttcta tttatgaaag gaaatatcat ggatagtttt gataaaggat ggtttgtttt
     6301 acaaacttat tctggttatg aaaataaggt gaaagaaaat ctattacaac gtgcacaaac
     6361 ctacaatatg ttggataata ttctacgcgt tgaaattcca acacaaacag tgcaagttga
     6421 aaaaaatgga aagagaaaag aagtagaaga aaatcgcttt ccaggttatg ttcttgtaga
     6481 aatggtcatg acagatgaag cttggtttgt tgttcgaaac acaccaaatg ttacaggatt
     6541 tgtcggatca cacgggaaca gatcaaaacc aactccatta ttggaacaag aaattcgtga
     6601 cattttggta tctatgggac aaactgttca agaatttgat ttcgatgttg agattggtca
     6661 aactgtacgt attattgatg gtgcttttgc agactacact ggtaagatta cagaaattga
     6721 taataacaaa gtgaaaatga ttatctctat gtttggtaat gacacagttg cagaagtaaa
     6781 cctaaaccaa atcgcagaat
//
LOCUS       DAVKLX010000076         6456 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKLX010000076 DAVKLX010000000
VERSION     DAVKLX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.076"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            1..437
                     /locus_tag="VW325_001991"
                     /pseudo
     CDS             1..437
                     /locus_tag="VW325_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            592..792
                     /locus_tag="VW325_001992"
                     /pseudo
     CDS             592..792
                     /locus_tag="VW325_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            817..1233
                     /locus_tag="VW325_001993"
     CDS             817..1233
                     /locus_tag="VW325_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735316.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1464..2171
                     /gene="ccdA2"
                     /locus_tag="VW325_001994"
     CDS             1464..2171
                     /gene="ccdA2"
                     /locus_tag="VW325_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9735317.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2182..2739
                     /gene="sdbB"
                     /locus_tag="VW325_001995"
     CDS             2182..2739
                     /gene="sdbB"
                     /locus_tag="VW325_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9735318.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIS
                     VGKDAPDFTLQSMDDKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3113..4504
                     /locus_tag="VW325_001996"
     CDS             3113..4504
                     /locus_tag="VW325_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9735319.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4647..5564
                     /gene="adcAII"
                     /locus_tag="VW325_001997"
     CDS             4647..5564
                     /gene="adcAII"
                     /locus_tag="VW325_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9735320.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDREHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5572..>6456
                     /gene="phtB"
                     /locus_tag="VW325_001998"
     CDS             5572..>6456
                     /gene="phtB"
                     /locus_tag="VW325_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEU9735321.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGL"
BASE COUNT         1984 a         1162 c         1363 g         1947 t
ORIGIN      
        1 gcttatttga ctgaaatagc ttctgaattt ggctgtcatc caactaccat ccactatgcg
       61 ctcaaagcta tgggctacac tcgaaaaaaa gaaccacacc tactatgaac aagacccaga
      121 aaaagtagcc ttatttctta agaattttaa tagattaaag cacctagcac ctgtttagat
      181 tgatgaaaca ggattcgata cttattttta tcgagaatat ggtcgctcat taaaaagtca
      241 gttaataaga ggcaaagtat ctggaagaag atatcagagg atttctttgg ttgcaggtct
      301 aacaaatgaa tggtgagtta atcgctccaa tggcttacga agagacgatg acgagcgact
      361 tttttgaagc ttggtttcag aagtttctct taccaacatt aaccacacca tcggttatta
      421 tagtaaaacg aaataagaat aggacaaatc gatcaggaca gtaaaatcga attctaacaa
      481 tgttttagaa gtccatatgt actattctag tttcaatata ccataactgc tcctagtcaa
      541 cctgcaagtc ctgccccagc tccaagctca gctccgactc aagatcctag agtggtagta
      601 agcgcttcgg ctagctcaac gagcacacaa gctcaagagt aagttgacaa gtctgaactt
      661 cgtgccttga gtcaagagtt agaccaacgc ttgaaagctt tggcaacagt atctgatccg
      721 aaaatcgatg caaccaaggc tgtcctccta gatgctcaaa aagctccgga agatagtgcc
      781 ttgacagagt aaggtctgca tactgcagta gagtctgtga aggctgcctt ggactctctc
      841 aaagatgtga aagcaaatgc tagcgacagc aagcctgcac aggacaagaa ggatgcaaaa
      901 caaggaacgg aagatagtaa ggattcagat aagatgactg aaacaaactc agttccggca
      961 ggagtgattg tggtcagtct acttgccctc ctaggcgtga ttgccttctg gctgattcgc
     1021 cgtaagaaag agtcagaaat ccagcaatta agcacggaat tgatcaaggt tctaggacag
     1081 ctagatgcag aaaaagcgga taaaaaagtc cttgccaaag cccaaaacct tctccaagaa
     1141 acccttgatt tcgtgaaaga agaaaatggc tcagcagaga cagaaactaa actagtagag
     1201 gagcttaaag caatccttga caaactcaag taaaacatga tttatgaaat gtcagaccta
     1261 aaagacattt tcgttcacaa aagcagtgac gagtaatcct tgtcgttgct ttttggctat
     1321 tttgggggat aagatccgcc ttgactagca aaagaaaaga ttggtccaat agtgcccgat
     1381 acgattttgc gtagcagaaa gcttacaaat aaaattgaat atagtaaaat aggattagaa
     1441 ttattaagaa agttggttct ttattggaaa cgatagtatt tttaatctct gtttttctag
     1501 caggtgtttt atcctttttt tctccttgta tttttcctct tctgccagtc tatgctggga
     1561 ttttattgga tgatcaagaa agtgcaaaaa gcttttcttt gtttgggaga aaggttctct
     1621 ggtcaggctt gattcgaaca ctttgcttta tcgctggtat ctctctcatt ttctttattc
     1681 taggctttgg tgctggttac tttggtcata ttctctatgc aaattggttt cgatatgtca
     1741 tgggagctat tattatcatt ttgggtcttc accagatgga aatttttcat ttgaagaaat
     1801 tagaagttca aaaaagtttt acctttaaaa aatcagattc taatcgttat tggtcagctt
     1861 ttttacttgg tattaccttt agctttggtt ggacaccttg tattggtcca gttttaagtt
     1921 ctgttttagc acttgcggct tctggaggca atggtgcttg gcaaggagcg atttatactc
     1981 tcatttacac tctgggcatg gcccttcctt tcttggtctt ggcactagct tcaggtctag
     2041 tcatgccata ttttagtaaa atcaagcgtc atatgatgct actaaagaaa attggtggtt
     2101 tcctcatcgt tttaatggga attttgttac tattaggaca agtaaatgtt ctagctggaa
     2161 tttttgaata aaggaggaca aatgaaaaaa gtaatgtttg ctggcttaag tctcttgtca
     2221 ttagttgtat tgatggcctg tggtgaggaa gaaactaaaa agactcaagc agcacaacag
     2281 ccaaaacaac aaacgactgt acaacaaatt tctgttggaa aagatgctcc agactttaca
     2341 ttgcaatcca tggatgacaa agaagttaag ttatctgatt ttaagggtaa aaaggtttac
     2401 ttgaagtttt gggcttcatg gtgtggtcca tgcaagaaaa gtatgccaga gttgatggaa
     2461 ctagcggcga aaccagatcg tgatttcgaa attcttactg tcattgcacc aggaattcaa
     2521 ggtgaaaaaa ctgttgagca attcccacaa tggttccagg aacaaggata taaggatatc
     2581 ccagttcttt atgataccaa agcaaccacc ttccaagctt atcaaattcg aagcattcct
     2641 acagaatatt taattgatag ccaaggaaag attggaaaga ttcaatttgg tgctatcagt
     2701 aatgcggatg cagaagcagc atttaaagaa atgaactaga atcgataaaa tctgattaca
     2761 agatgtagtc agattttggt aacttctaaa actaacttcc agtttcccac aacctagctt
     2821 ttagtatgag aaaaacgcgt gacatcagtg gaaatccgtc ttagactaac ttccactggt
     2881 tttctttttc ttgatatata agggttcaaa agcgaaaaga ctcagaaaaa gtgcacgaca
     2941 aatagcccta aaacagagtc gtcttagagt aacttccagt tttgggagag agatggaagt
     3001 tactttgaga agttacggta gtcagatttt tttagaaaaa cattttataa atattcacat
     3061 atctcctata tttatggtaa aatagaatta tcagtttatt ttggagtcaa agatgaatat
     3121 atttagaaca aagaatgtta gtttagataa aacagagatg cataggcatt tgaagttatg
     3181 ggatttgatt ttgctgggta tcggagccat ggtagggaca ggcgtcttta caatcacagg
     3241 tactgcagct gcaacacttg ctggcccagc cctagtgatt tcaatcgtta tttctgcctt
     3301 gtgtgtggga ttatcagccc tcttttttgc agaatttgcc tcgcgagtac ccgctacagg
     3361 aggtgcctat agttacctct atgctatctt aggagaattc cctgcctggt tggctggttg
     3421 gttaaccatg atggagttca tgacagccat atcaggcgta gcttcgggtt gggcagctta
     3481 ttttaaaggc ttgctctctc aatacgggat agcctttcct caggctttaa atggtacctt
     3541 taatcctcaa gcagggacat ttgttgattt attgcctatt cttgtcttgg tcttggtgac
     3601 ttcgcttgtt ttactaaatg ccaaggcagc cttacgcttt aattcgattc tagttatttt
     3661 gaaattttcc gctttagctc tctttgtttt agtaggaatt tggaatatta agtttgataa
     3721 ttggagcaat tttgctcctt acggttttgg acaaatctat ggagctagta ctggtattat
     3781 ggctggtgca tccttgatgt tcttcggttt tttgggattt gaatccatct ctatggctgt
     3841 agatgaagtc aagactcctc aaaaaaatat tcctagaggg attgtcttat cgctttctat
     3901 cgtaaccatt ctctatgcct tggtgaccct tgttttgact ggtgtggttc actatagtca
     3961 tttaaatgtc gatgatgccg ttgcctttgc ccttcgtagt gttgggatta gttgggcagc
     4021 caactatgtg tcattagtgg ctatcttgac cttgattaca gtttgtattt cgatgaccta
     4081 tgccctatcg cgtatgattt acagtttagc acgtgacggc ttagtgcctg ctgcctttaa
     4141 ggaactgacg aagactagca agataccaaa gaatgctact atcttgacag gtctagcttc
     4201 agcagtagca gcaggaatgt tcccactagc cagtattgca gcctttttaa atatttgtac
     4261 cttagcctac ttgatcatgt tggcttatgg tctgattcgc ttacggaaag aaaaaggaat
     4321 gcccaaagct ggggaattta aaacaccatt ggtacctctg ttaccaattt tatcgatcat
     4381 catttgttta tccttcatgt tacagtataa tatgaatacc tggattgctt tcctagttgc
     4441 gttgttagta ggaagtatta tttacttcac ttatggctat aagcattcta ccatagaaga
     4501 ataaagtgaa agtctattga gttgttgaca ctcagtagat ttttatatgt gaaagaaatt
     4561 tcaaattttt tctaaaaata tcttgacaga ttaaattttc aggagtagaa tatttactag
     4621 ttaattaaat ggttaaggag ttgttcatga agaaacaaaa tttattttta gtcctgttaa
     4681 gtgtctttct tttgtgcttg ggggcttgtg gtcaaaagga aagtcagaca ggaaagggga
     4741 tgaaaattgt gaccagtttt tatcctatct acgctatggt taaggaagta tctggtgact
     4801 tgaatgatgt tcggatgatt cagtcaagta gtggtattca ctcctttgaa ccttcggcaa
     4861 atgatatcgc agccatctat gatgcagatg tctttgttta ccattctcat acactcgaat
     4921 cttgggcagg aagtctggat ccaaatctaa aaaaatccaa agtgaaggtc ttagaggctt
     4981 ctgagggaat gaccttggaa cgtgtccctg gactagagga tgtggaagca ggggatggag
     5041 ttgatgaaaa aacgctctat gaccctcaca catggctaga tcctgaaaaa gctggagaag
     5101 aagcccaaat tatcgctgat aaactttcag aggtggatag ggagcataaa gagacttatc
     5161 aaaaaaatgc gcaagccttt atcaaaaaag ctcaggaatt gactaagaaa ttccaaccaa
     5221 aatttgaaaa agcgactcag aaaacatttg taacacaaca tacagccttt tcttatctag
     5281 cgaagagatt tgggcttaat caacttggta ttgcaggtat ctctcctgaa caagaaccaa
     5341 gtccacgaca actaacagaa attcaggaat ttgttaagac ctataaggtt aaaacgattt
     5401 ttacagaaag taacgcttct tcaaaagtag ctgaaactct tgtcaaatca acaggtgtgg
     5461 gtcttaaaac tctgaatcct ttagagtcag acccacaaaa tgacaagacc tatttagaaa
     5521 atcttgaaga aaatatgagt attctagcag aagaattaaa gtgaggaaag aatgaaaatt
     5581 aataaaaaat atctagcagg ttcagtggca gtccttgccc taagtgtttg ttcctatgag
     5641 cttggacgtt accaagctgg tcaggataag aaagagtcta atcgagttgc ttatatagat
     5701 ggtgatcagg ctggtcaaaa ggcagaaaac ttgacaccag atgaagtcag taagagggag
     5761 gggatcaacg ccgaacaaat tgttatcaag attacggatc aaggttatgt gacctctcat
     5821 ggagaccatt atcattacta taatggcaag gttccttatg atgccatcat cagtgaagag
     5881 ctcctcatga aagatccgaa ttatcagttg aaggattcag acattgtcaa tgaaatcaag
     5941 ggtggttatg tcattaaggt aaacggtaaa tactatgttt accttaagga tgcagctcat
     6001 gcggataata ttcggacaaa agaagagatt aaacgtcaga agcaggaacg cagtcataat
     6061 cataactcaa gagcagataa tgctgttgct gcagccagag cccaaggacg ttatacaacg
     6121 gatgatgggt atatcttcaa tgcatctgat atcattgagg acacgggtga tgcttatatc
     6181 gttcctcacg gcgaccatta ccattacatt cctaagaatg agttatcagc tagcgagtta
     6241 gctgctgcag aagcctattg gaatgggaag cagggatctc gtccttcttc aagttctagt
     6301 tataatgcaa atccagctca accaagattg tcagagaacc acaatctgac tgtcactcca
     6361 acttatcatc aaaatcaagg ggaaaacatt tcaagccttt tacgtgaatt gtatgctaaa
     6421 cccttatcag aacgccatgt ggaatctgat ggcctt
//
LOCUS       DAVKLX010000077         6411 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKLX010000077 DAVKLX010000000
VERSION     DAVKLX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.077"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            191..1957
                     /gene="patB"
                     /locus_tag="VW325_001999"
     CDS             191..1957
                     /gene="patB"
                     /locus_tag="VW325_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9735322.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2096..2785
                     /locus_tag="VW325_002000"
     CDS             2096..2785
                     /locus_tag="VW325_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9735323.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            2789..2968
                     /locus_tag="VW325_002001"
     CDS             2789..2968
                     /locus_tag="VW325_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735324.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            2955..4304
                     /locus_tag="VW325_002002"
     CDS             2955..4304
                     /locus_tag="VW325_002002"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9735325.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVIIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4428..5888
                     /locus_tag="VW325_002003"
     CDS             4428..5888
                     /locus_tag="VW325_002003"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9735326.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEEFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            5902..6336
                     /locus_tag="VW325_002004"
     CDS             5902..6336
                     /locus_tag="VW325_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9735327.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVSELIPYWWGEERASKFNER"
BASE COUNT         2022 a         1213 c         1382 g         1794 t
ORIGIN      
        1 ttacaagaga taaaacgttt atgcgttatc aaactcatta ccaattgaaa caaaaagctg
       61 tggttagatc ctttcggaaa ttgtcaagcg attggaggaa atgaactaat ccacagcggc
      121 ttattccaag tataccactt gggctttggc agtagctaac tgcgctaaat ataatataag
      181 gaggagtaaa atgaagacag ttcaattttt ttggcattat tttaaggtct acaagttctc
      241 atttgtagtt gtcatcctga tgattgttct ggcgactttt gcccaagccc tctttccagt
      301 cttttctgga caagcggtga cgcagctagc caatttagtt caagcttatc aaaatggcaa
      361 tccagaactt gtatggcaaa gcctatcagg aatcatggtc aatcttggcc tgctggtttt
      421 ggttctattt atctctagtg taatatacat gtgtctcatg acgcgcgtga ttgcagaatc
      481 gaccaacgag atgcgcaaag gcctctttgg taagcttgct cagttgacgg tttctttctt
      541 tgaccgtcga caagatggcg atatcctgtc tcattttacc agtgatttgg ataatatcct
      601 ccaagccttt aacgaaagct tgattcaggt catgagcaat attgttttat acattggtct
      661 gattcttgtc atgttttcga gaaatgtgac gctggctctc atcaccattg ccagcacccc
      721 attggctttc cttatgctga ttttcatcgt gaaaatggca cgtaaataca ccaacctcca
      781 gcagaaagag gtagggaagc tcaacgccta tatggatgag agcatctcag gccaaaaagc
      841 cgtgattgtg caaggaattc aagaggatat gatggcagga tttcttgaac aaaatgagcg
      901 cgtgcgcaag gcaaccttta aaggaagaat gttctcagga attcttttcc ctgtcatgaa
      961 tgggatgagc ctgattaata cagccatcgt catctttgct ggttcggctg tacttttgaa
     1021 tgataagtct attgaaacaa gtacagccct aggtttgatt gttatgtttg cacaattttc
     1081 acagcagtac taccagccta ttatccaagt tgcagcgagt tggggaagcc ttcagttggc
     1141 ctttactgga gctgaacgaa ttcaggaaat gtttgatgca gaggaggaaa tccgacctga
     1201 aaaggctcca accttcacta agttgcaaga aagtgttgaa atcagtcata tcgatttttc
     1261 atacttgcct gataaaccta ttttgaaaga tgtcagcatt tctgccccta aaggccagat
     1321 gacagcagtt gttgggccta caggttcagg aaaaacgact attatgaacc tcatcaatcg
     1381 cttttatgat gttgatgctg gtggtattta ttttgatggt aaagacattc gtggctatga
     1441 cttagatagt cttagaagca aggtgggaat tgtattgcaa gattcggtct tgtttagcgg
     1501 aacgattcga gacaatatcc gatttggtgt gccagatgct aatcaggaaa tggttgaggt
     1561 agcagcaaaa gcaacccaca ttcacgacta tatcgaaagt ttgcctgata agtacgatac
     1621 tcttattgat gatgaccaga gcatcttttc aacagggcag aagcaattga tttcaatcgc
     1681 tcgaaccctg atgacagatc cagaagttct cattctcgat gaagcaactt caaacgtaga
     1741 tacggtgacg gaaagcaaga ttcagcatgc catggaggtg gttgtagcag gtagaactag
     1801 tttcgtcatt gcccaccgct tgaaaaccat tctcaatgca gatcagatta ttgtccttaa
     1861 agatggagaa gtcattgaac gtggtaacca ccatgaactt ttgaagctag gtggctttta
     1921 ttcagaactc tatcacaatc aatttgtttt cgaataagaa agaagttgtc ctatgtgggc
     1981 agctttttct tgtccataaa aaatgtttat cacagcctta aaaaaaacat attagacgaa
     2041 agtcattttg agtgatatga taggactatc gttagtattc gaaaggagag gcatcatggc
     2101 tagaacggtt gtaggagttg ctgcaaatct atgtcccgta gacgcagaag gcaaaatcat
     2161 tcattcatct gtatcttgta gattcgcaga gatcattcgt caagtcggtg gtctcccttt
     2221 agtcattcct gttggtgatg agtcagttgt acgtgattat gtggaaatga ttgacaaact
     2281 cattttgaca ggaggccaaa atgttcatcc tcagttttat ggagagaaaa agaccgtcga
     2341 aagcgatgat tacaatctgg tccgtgacga atttgaattg gcactcttga aggaagcgct
     2401 tcgtcagaat aaaccaatta tggcaatctg tcgcggtgtc caacttgtca atgttgcctt
     2461 tggtggaacc ctcaatcaag aaatcgaagg tcactggcaa ggactacctt tcgggacatc
     2521 tcactctatt gagacagtag aaggaagcgt ggtggctaag ctatttggga aagaaagtca
     2581 ggtcaactca gtccatcgtc aaagcattaa agatttggca cctaatttcc gtgtaactgc
     2641 tattgattca agagaccaaa ctattgaagc gattgagtct atcgacgagc accgcattat
     2701 cggtttacag tggcatccag agtttctggt taatgaagaa gatggcaatt tagaattatt
     2761 tgagtattta ttgaatgaac tgtaacgatt ggaacatctg gtcgttcttt cttttatttt
     2821 ttcaaaattt ttagaatgtg gcattttacg caaacgtttg aattctgata aaaattggag
     2881 aaattcgaca aaaaaacttg aaaaaaacga aggtaagcgt tatgatagaa aagaagaaat
     2941 attggaggaa gaacatgtca catattaaat ttgattattc aaaagtttta gacaaatttg
     3001 ttgcaccaca tgaagtggaa tacatgcaat cacaagtaac agcagcagat gaattgatcc
     3061 gtaaaggaac tggtgctggt agcgacttct tgggatggtt ggaccttcct gaaaaatacg
     3121 accgcgaaga atttgaccgc atcttgaaag ctgctgagca aatcaagtca gacagcgatg
     3181 ttttggttgt gatcggtatt ggtggatctt accttggtgc aaaagcagca atcgacttct
     3241 tgaatcacca ttttgctaac ttgcaaacaa aagaagaacg caaagctcca caaatccttt
     3301 acgcaggaaa ctcaatctca tctacttacc ttgctgactt ggtagagtat gttgcagaca
     3361 aagacttctc agtaaacgta atttctaaat caggtacaac aactgaacca gcgattgctt
     3421 tccgtgtctt taaagaactc ttggttaaga aatacggtca agaagaagct aataaacgta
     3481 tctatgcaac aactgaccgc caaaagggtg ctgttaaggt tgaagcagac gctaacggtt
     3541 ggggaacatt tgttgttcca gatgatatcg gtggacgctt ctcagtattg acagccgttg
     3601 gtttgcttcc aatcgcagca tcaggagctg acataaaagc tcttatggaa ggtgcgaatg
     3661 cagctcgcaa agactacact tcagacaaaa tctctgaaaa cgaagcttac caatacgcag
     3721 ctgttcgtaa catcctttat cgtaaaggct atgcaactga gatcttggta aactatgagc
     3781 catcacttca atacttctca gaatggtgga aacaattggc tggtgaatca gaaggaaaag
     3841 accaaaaagg tatctaccca acttcagcca acttctcaac tgacttgcac tcacttggtc
     3901 aatttatcca agaaggaact cgtatcatgt ttgaaacagt tgtccgtgtt gacaaacctc
     3961 gtaaaaacgt gattattcct actttggaag aagaccttga cggacttggt taccttcaag
     4021 gaaaagacgt tgactttgta aacaaaaaag caactgacgg tgttcttctt gcccacacag
     4081 atggtgatgt accaaacatg tatgtgactc ttccagagca agacgctttc actcttggtt
     4141 acactatcta cttcttcgaa ttggcaattg ccctttcagg ttacttgaat gctatcaacc
     4201 catttgacca accaggtgtt gaagcttata aacgtaacat gtttgccctt cttggaaaac
     4261 caggatttga agaattgagc aaagaactta acgcacgtct ataatagaag aaaagagtgg
     4321 tttgcccact ctttttactc tctttatcca tagaaattgg actcagccaa gacttgtgat
     4381 ataatataga aagcaaaaag gcagacgcct agataatagg agaaactatg tcaaaagata
     4441 tccgcgtacg ttacgcacca agtccaacag gactactaca catcggaaat gcccgtacag
     4501 cattgtttaa ctacttgtat gcgcgccatc atggtggaac atttctcatc cgtatcgaag
     4561 atactgaccg taaacgccat gtcgaggatg gtgaacgttc acaacttgaa aaccttcgct
     4621 ggttaggcat ggattgggat gaaagtccag aatcacatga gaattatcgc cagtctgagc
     4681 gtttggactt gtatcaaaaa tacattgacc aactattagc tgaaggaaaa gcctataaat
     4741 cttacgttac agaagaagag ttggcagctg aacgtgaacg tcaagaagct gctggtgaaa
     4801 cacctcgata tatcaacgaa taccttggta tgagtgaaga ggaaaaagcg gcttatatcg
     4861 cagaacgtga agcagctggt atcataccaa cagttcgttt ggctgtcaat gagtcaggta
     4921 tctacaagtg gcatgatatg gtcaaaggtg atatcgaatt tgaaggtggc aatatcggcg
     4981 gtgactgggt tatccaaaag aaagacggtt acccaactta caactttgcc gttgttatcg
     5041 atgaccacga tatgcaaatc tctcatgtta tccgtggaga tgaccatatt gctaatacac
     5101 caaaacagct tatggtctat gaagctcttg gttgggaagc tccagagttt ggtcacatga
     5161 ccttgattat caactctgag actggtaaga agttatctaa acgtgatact aatacacttc
     5221 aatttatcga agattaccga aagaaaggtt accttccaga agcagtcttt aactttattg
     5281 ctcttcttgg ttggaaccca ggtggtgaag atgagatttt ctctcgtgaa gaactcatta
     5341 aactttttga tgaaaaccgc ctcagcaagt caccagcagc ctttgatcag aaaaaattag
     5401 actggatgag caatgattat atcaagaatg cagaccttga aaccatcttt gaaatggcaa
     5461 aaccattctt agaggaagca ggccgtttga ctgacaaggc tgaaaaatta gttgagctct
     5521 ataaaccaca aatgaaatca gtagatgaga ttatcccatt gacagatctt ttcttctcag
     5581 atttcccaga attgacagaa gcagagcgcg aagtcatgac gggtgaaaca gttccaacag
     5641 ttcttgaaga attcaaagca aaacttgaag cgatgacaga tgatgaattt gtgacagaaa
     5701 atatcttccc acaaattaaa gcagtccaaa aagaaacagg tattaaaggg aaaaatcttt
     5761 tcatgcctat tcgtatcgca gtttcaggcg aaatgcatgg gccagaatta ccagatacaa
     5821 ttttcttgct tggacgtgaa aaatcaattc agcatatcga aaacattcta aaagaaatct
     5881 ctaaataaag aggatacaat aatggacatc tgggaaaaga tgtacgaaga agcacagaaa
     5941 ctatacaatc cacatgaagt atcagacttt gtttatgcca atcatgttgt agctgcagta
     6001 gaagcaggag atggacaaat atttacagga ttctgtatgg agggaacctg tggtgttttc
     6061 catctctgcg cagaacgggc ggccctcttc aatatgtacc aattttcagg acaaactaag
     6121 gttaagaaag tattagcctt tcgagataaa ccaccttatg gtggaagttc agccatgcct
     6181 tgcggtgctt gtagagaatt ccttttagag cttaatgctg aaaataaaga tgcagaattc
     6241 atgatggact ataatataag aaaaacagtt aaagtctcag aactaatccc ttattggtgg
     6301 ggagaagaac gtgcttctaa gtttaatgag cgatagaaga gtcagtataa ttctggctct
     6361 ttttactata agttgaaaaa ataaaaaaaa ttagaaaaag tagttgacaa a
//
LOCUS       DAVKLX010000078         5049 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKLX010000078 DAVKLX010000000
VERSION     DAVKLX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.078"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..370)
                     /locus_tag="VW325_002005"
     CDS             complement(<1..370)
                     /locus_tag="VW325_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735328.1"
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IRKDFDIKLTNEYKKISQFRS"
     gene            complement(367..720)
                     /locus_tag="VW325_002006"
     CDS             complement(367..720)
                     /locus_tag="VW325_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9735329.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLAGAAGRIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
     gene            1479..1799
                     /locus_tag="VW325_002007"
     CDS             1479..1799
                     /locus_tag="VW325_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9735330.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(1851..2438)
                     /locus_tag="VW325_002008"
     CDS             complement(1851..2438)
                     /locus_tag="VW325_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9735331.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            2680..3351
                     /gene="sdaAB"
                     /locus_tag="VW325_002009"
     CDS             2680..3351
                     /gene="sdaAB"
                     /locus_tag="VW325_002009"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9735332.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            3360..4232
                     /gene="sdaAA"
                     /locus_tag="VW325_002010"
     CDS             3360..4232
                     /gene="sdaAA"
                     /locus_tag="VW325_002010"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9735333.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            4254..4886
                     /locus_tag="VW325_002011"
     CDS             4254..4886
                     /locus_tag="VW325_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9735334.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
BASE COUNT         1557 a         1059 c          899 g         1534 t
ORIGIN      
        1 tgcttctaaa ctgactaatt ttcttatatt cgttagtcaa tttaatatca aaatctttcc
       61 taattaaatg aaagattgct aactctagga taaaaacact tattaatacg gtataagtca
      121 aaaaactttc tgggaaaaac ttattgatta aaatggggaa actcattaac agaatcgaat
      181 ttatggctag acagatagat actattttat ttttcacaga atatagaggt agtgatacta
      241 ttgagtagat aaaagctaaa aaaataagca aaaaatactc cgtgtcattt ataatcgccc
      301 tgaatgataa aaatatatac atccaacata cgataatgtg tatttttcga atcttatcta
      361 cattactcat cttactgtcg cagatatgaa tccatcccaa ggataagggt tcccgctaat
      421 atcacaacct ctattaagag caccatatcc aatacagtaa gctattcttc ctgccgcccc
      481 agccaataca cctgctgctg caccaataag ccaacctcca aggcctccaa ctttaatata
      541 gtttattagt tttttggtta aaactgcagc tccagctaat gctataaaag ttcctggact
      601 catagctaaa gctgcgcaaa ttccttgaca agcacgcttg cttaaataag ctcccccatc
      661 aaggtacatc atctcttctt catcaatcac aacataatta tttggtaata caagttccat
      721 ttgttttctc cttttttatt aagcatggtt aggatggttt cctaacaagt gatcgcaggc
      781 tttgtttagt tacctaaatt agacttcctt tctttatctt attttaaata tttaaaacca
      841 gttactgctt gaagcttaac agtttactta attcaaataa cagctaatca ctgattccct
      901 gattttatca aatatactaa taacaacaat tgtgatacga tcatcattat aagaacaaca
      961 tttaacgaat caaagttttt ccgagataat aataaagcaa cagcaagcag tattattact
     1021 aaagattgaa taactactat tactcttaac attttatcct ccactaacaa ctcaagctcc
     1081 cttacattat atcgaccagc cccatggaaa gattatagct agactaatca atgatataat
     1141 acatactaga atacaaaata tttttttatt catacttttt ctcctatctt tattttctac
     1201 ctacatagta cctcttatta agctcatttc aaaattacgt taactttttt cacgaaaagg
     1261 agaaaattat gcgttatgat tatggatagc gctttcaaat ttttaaactc ttatcccatc
     1321 cttttatcta tataataagt gaaaatataa taactgtcaa gtaatcgaag tgaattttat
     1381 aaaaaaaatt acaagccaaa tttgcaaagt ttacaataag ccgctagggc aatcgtctat
     1441 cagaatgtcc gtttatttgt caataatccg agaaaatctt gcaacgctta gaagtctata
     1501 aaaactatca acatttatat gacttgcgaa tagcaatcct gctcaacctt tccacactat
     1561 atctatacaa tcaagataaa aacatgtgta agcaaatctg ctacacttta ctggaggacg
     1621 ccaagaataa gaaaagctac gataggctgg ctatctgcta tgtccgcatt gggatttgta
     1681 cagacgattc taaacttatc caaaaaggtt tctcactcct agagctgacc gaggaaactt
     1741 ctatgctgtc tcatctcaaa aaagaagtag agatctatta ccaagcgaag gaaagataaa
     1801 aaagccgagg gatttcctcg actctttaca tattatttgt tcgttagttc ttaataccag
     1861 ccattgttaa gccagaagtt tttagcagca gtccatgaac cgtaacgtcc tgcaacgtag
     1921 gcatctgcta cacgttcttg gttttcagct gagtagtcac cgttcaggta tgaatctgtt
     1981 aattggtaac gtccgatata acgtccattt gtagctgtat agctaccacc tgattctttt
     2041 tgagcgatcc attctttggc ttctgcttca gatccgctta cagttgaaac aactgtttca
     2101 cttactactg gagtttctgc tactggagct gaaacagttt catcttgagc ggctggtgcc
     2161 gcataagtag ctggcgctgg tgttgcaaca ggcgctacag ggccatcgat aaccaactct
     2221 tgatcaacat aaatcaaatg aatgttatca atgtggttgt tttctgccaa tttttcaact
     2281 gttgtgttgt gagtttcagc gatttctgaa agtgtatcac cttctttaac agtgtaagtt
     2341 gatgattctt gagcagatac aaatgatgga gcaaatactg caaacaaggc agctactcct
     2401 gcaagagttg ctttaatctt tttagttatt gatttcatat tcgaaaattc tccttctttc
     2461 tatagttcta tcataccctt taaatattac cgtttcttga cacttttatg tagaaatatt
     2521 acaaaattat tttttatttc cctaaataag gtgttttttg tcataaaaga tactattttt
     2581 atgctatttg aggtattaga aaggttttca tccacaatta aagccaagcc cctcattctt
     2641 tgatataata gagataagaa tttttataag gggaaactga tgaaatcact tcgttttcaa
     2701 tctgtctttg atatcatcgg accagttatg attggcccat ctagtagcca taccgctggt
     2761 gctgttcgta ttgggaagat tgtctcttcc atttttgatg atactccgac agaagttgaa
     2821 ttccaacttt ttaactcatt tgccaagacc tatcgtggtc acggaacaga cctagccctt
     2881 gttgcaggta ttttaggaat ggatacagat gatcctgaaa ttccaaatag tctggaaatt
     2941 gcccacaagc gtggtatcaa gattgtctgg accattcaga aagacagcaa tgcccctcac
     3001 cctaacacca ctaaaattac tgttaaaaat gcccacaaga ccatcagcgt gactggtatt
     3061 tctatcggtg gaggaaatat tcaagtaacc gaactcaatg gttttgccgt ctctctcaat
     3121 atgaatacac cgactatcat catcgttcat caagatattc caggtatgat tgcccttgtt
     3181 acagaggcgc tttcccgcta tggtatcaat atcgcccaga tgaatgtcac tcgtgaaaaa
     3241 gctggtgaaa aagccattat gattatcgaa gttgacagtc gcaactgtga tgaggctatc
     3301 gaagaaattc gaaaaatccc tcatctccac aatgtcaatt tctttaaata ggaggaagca
     3361 tgttttattc tatcaaagaa ttggtcgagc aagcagatct ggactttcaa ggaaatgtcg
     3421 cagaactcat gattacaaca gagtttgaat tgaccggtcg cgaacgtgaa gaagtcttcc
     3481 ttctcatgga acgcaatctg gaagttatga aagcctctgt ccaacttggc ctcaatgaaa
     3541 ataaatctcg tagtggcctg acaggtggag atgctgccaa attggatcac tacatcgaaa
     3601 acggaaaaac tctgtcagat tatacgattc tctcggctgc ccgaaatgcc atcgcagtca
     3661 atgaacacaa tgccaaaatg ggcttggtct gtgccactcc aaccgctgga agtgctggct
     3721 gtctgccatc cgttctcact gctgctattg aaaaattaga cctcagccac gagcaacaat
     3781 tggatttcct ctttgctgct ggtgcctttg gactagtcat cgcaaacaat gcctccatct
     3841 caggtgctga gggtgggtgt caagctgaag ttggttcagc ctctgctatg agtgctgccg
     3901 ccttgactct ggctgcaggt ggaacacctt atcaggccag tcaagctatt gcctttgtca
     3961 ttaaaaatat gctaggcctc atctgtgacc ctgttgcagg tttggtcgaa gttccctgtg
     4021 tcaaacgtaa tgccatggga gctagctttg ctttcatcgc agcagacatg gccttggcag
     4081 gtatcgaatc taaaatccct gtggatgaag tgatcgatgc catgtaccaa gtaggagcaa
     4141 gcatgccaac tgcctttcgt gaaacagctg aaggtggact cgctaccacc cctactggtc
     4201 gtcgcctcca aaaagaaatt ttcggagaat aagcttatca aataggagaa accatgacct
     4261 ctatcacagc gatttttttc gatttggatg gaaccctcgt tgatagttct atcgggattc
     4321 acaatgcctt tacctatacc tttaaagagc tgggggtgcc tagccctgat gccaaaacta
     4381 ttcgtggttt tatgggacca cctctcgaaa gtagttttgc gacctgcctg tccaaagacc
     4441 aaatttctga agccgtgcag atatatcgtt cttactataa ggcaaaaggc atctatgaag
     4501 ctcaactctt tcctcagatt atagacttgc ttgaggagtt atcgagcagt tatccactct
     4561 acatcaccac gacaaaggat acatctactg ctcaagacat ggccaaaaac ttggaaatcc
     4621 atcatttctt tgatggcatt tatggttcta gccctgaagc accccataag gcagatgtca
     4681 ttcaccaagc cttgcagaca catcaactag caccagaaca agccatcatc atcggagata
     4741 ccaagtttga tatgctggga gctcgagaaa caggtattca gaaattggcc atcacttggg
     4801 gatttggaga gcaagcagat ctactaaact atcaacctga ttatatcgct cacaaaccct
     4861 tagaagtttt ggcgtatttt caatagcata gactgggtaa caattccatt tcagaagaaa
     4921 atgaagtaat actcaatgaa aatcaaagag caaactagga aactagctgc gggctgctca
     4981 aaacactgtt ttgaggttgc agatagaact gacgaagtca gctcaaaaca ctgttttaag
     5041 gttgtggat
//
LOCUS       DAVKLX010000079         4940 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKLX010000079 DAVKLX010000000
VERSION     DAVKLX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.079"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            81..608
                     /locus_tag="VW325_002012"
     CDS             81..608
                     /locus_tag="VW325_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735335.1"
                     /translation="MIKYKELKSYIDEWRYYSDENNPRLDLEYCKTKIVEKAKEFDLP
                     CQIDEEQIKLGGLFNKEIEECLVISHPNHQKDYVKFCFRLKNQGSVQLLTIDTLGESK
                     QLKKYYISEDNKRFREAIRESDLSLGQKLGAQLSNLTVSGLRTLGKNQSKIDAELQYC
                     EFLTEVLTQFRSNND"
     gene            618..2156
                     /locus_tag="VW325_002013"
     CDS             618..2156
                     /locus_tag="VW325_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU9735336.1"
                     /translation="MSKAIGIDLGTTYSAVSVLSETGQPQILLNQDGENLTPSVVFFQ
                     DFDGKDEPLVGIQAKNLAASRPEAVVQYVKRQMGNPNWKFDSPSDTVYTAEEISAIIL
                     KRLKEGAENALGDKVEDVVITVPAYFDDARRTATKHAGEIAGLNVLRVLNEPTAAALA
                     YGISAEKNETVLVYDLGGGTFDVTLMKIKDGEFDVIATDGDRNLGGFDFDNALSMIIA
                     EKMEEQGAEDIYTDEHFTALLREKSENTKRGLTTVEKTNVFLDYKGKSYKIPITRVEF
                     EEATKSLMNRTEELLDDVVEESGMSWDEIDQVLLIGGSTRMPMVQRKLEEKIGKKIVY
                     SINPDEAVAQGAAIQAALEIANKSESADVSETVRGLAEKLVVSDVTSQALGTLALSNG
                     VKRNTIIIPKNSKVPNKYSEYFSTVVDNQQNVLVEVTQGDDEDPQFVTVIGESTIKLP
                     GDWPAGTVLKVTYHYDVDQTVFVEVHDSDNHLLGTFEVERQANLDKSAVEVASRKIKD
                     LTID"
     gene            2169..3536
                     /locus_tag="VW325_002014"
     CDS             2169..3536
                     /locus_tag="VW325_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9735337.1"
                     /translation="MSVINYYEELGISETSSLDDVKKSIKSNRRRYRQLTGSPNIDQR
                     SMAERKMEVIAQAEKVFESEETRQKYDRELENSKQSSEGVPDSTPTNHSNSSYLDSAR
                     QAFYSGKKSLAYSYIEEALKINRNDADVWYFKAMISLEDRKLSDAELAISEANRLRPK
                     NADILSLLGDVYCEQNQQKFAIQYYQEAFELSNNSFYLLKKGRSLFLFDQYKQAVKDV
                     RYLYDHYLEENKNNEEYIYYLLSCSYREIGDNEEAYNFARKLVDVSDSLEHKLHYAGI
                     LFLKSEDECENYLDTLNERYGNNKDFQQVYFKLLLDITIERMKKAPDTDVEAFFRKYI
                     DRTKYENEAVLDNKARVRIAEAYLAKAQSLGIENKIIDNDFYNEFIENKNYINYATTR
                     HSQGMGCLMFILVFAANWFLSGFFDKFVHSFWISNALSLALLSNIVLYYNYPKGWQIN
                     RRKKG"
     gene            3544..4002
                     /gene="grpE"
                     /locus_tag="VW325_002015"
     CDS             3544..4002
                     /gene="grpE"
                     /locus_tag="VW325_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9735338.1"
                     /translation="MSEEVLHEKVSYIVDKVEDIESLITRRLLEDKAKNSLIEELKQY
                     LIYRQDLDKGEKFAPFMKQILQVIDRIESSEEKSDLLTSIAEELLQILSLNGLQVIDN
                     SGMIDPSMHEVVNTVAVTDEQSENNIVEVLQKGYLLNNRVLRPSKVTIAK"
     gene            4027..4839
                     /locus_tag="VW325_002016"
     CDS             4027..4839
                     /locus_tag="VW325_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HEU9735339.1"
                     /translation="MLRFFKHRRNILYFILGFLWGRRQNAKVSPEPPTPSTPKHSELP
                     SISKATHNGKMTGFEPQKLKTYQLYQHELMFGEPGKGLNKSGFDESAVNLGQEGEINF
                     AKALQKKGLLEKLVTFWSVHNLNLEDERVDADIDCVIVSGSTIWLVDLKFYASGNVIY
                     READGLLYTIDSATGAQIGRPKKMSPNMSYAEESFSHKFASLLKYYRLETRVVLMPTY
                     KGAGRLDNVFWPGHIKAVSLEEMLDELSREDKFRDTIGGQMIRQTFNLLLKR"
BASE COUNT         1795 a          637 c         1016 g         1492 t
ORIGIN      
        1 ataaatacgc ttttcgtaaa tcgagcgaag gctatgctat actgtaaccg taaacaataa
       61 gatataaaag gagattgttg atgattaaat acaaagaatt gaaaagttat atagatgagt
      121 ggagatatta ttccgatgag aacaatccaa gattggattt agaatattgc aagacgaaaa
      181 ttgttgagaa agcaaaagag tttgatcttc cttgtcaaat tgacgaagaa caaataaaac
      241 tcggcggttt gttcaataaa gagattgaag aatgtctagt tatttcgcac ccaaatcatc
      301 agaaagacta tgtaaagttt tgttttcgtt taaaaaatca aggatcagta caacttctga
      361 ctatagatac tttaggtgag agtaaacaat taaaaaaata ctatatatca gaagataata
      421 aaagattcag agaagcaatt agagaatcgg acttgtcatt gggtcagaaa ttaggagctc
      481 aacttagtaa cttaacagtt agcggtctta gaactttggg gaaaaatcag agcaagattg
      541 atgcagaatt acaatactgt gagtttttaa cagaggtact aacacagttt agatcaaata
      601 atgattagga gtgaaatatg agcaaagcaa ttggaattga tttaggaacg acttattcag
      661 cagtatctgt attatcagag actggacaac cacaaatttt attgaatcaa gatggtgaaa
      721 atttaactcc atctgttgta ttctttcagg attttgatgg gaaagatgaa ccacttgttg
      781 gtattcaagc taaaaatttg gcagcaagta gacctgaagc agttgtacag tatgtcaaaa
      841 gacaaatggg aaatccaaat tggaagtttg attctcctag tgatactgtt tatacagcag
      901 aggaaatttc agcaatcatt ttaaaaagat taaaagaagg agcagaaaat gcccttggtg
      961 acaaagtaga ggatgttgta ataactgttc cagcttattt tgatgatgcc cgtagaacag
     1021 caacgaaaca tgctggagaa attgctggac ttaatgtttt acgagtttta aatgaaccga
     1081 cagccgctgc acttgcatac ggtatatcag cagaaaaaaa tgagacagta ttggtatatg
     1141 atttaggtgg tggaactttt gacgtcacat taatgaaaat aaaagatggc gagtttgatg
     1201 ttattgccac cgatggagat cggaatctag gagggtttga ttttgataac gccttatcaa
     1261 tgattattgc tgaaaagatg gaagaacagg gagcagaaga tatttatacg gatgaacatt
     1321 ttactgcctt acttcgtgaa aaatcagaga atacaaagag aggactaaca actgtagaaa
     1381 aaacaaatgt ctttctcgac tataaaggaa aaagttataa aattccgata acacgtgtag
     1441 aatttgaaga agcaacaaaa agtctaatga atcgtacaga ggagctgtta gatgatgttg
     1501 ttgaagagtc tggaatgtca tgggatgaaa ttgaccaagt actattaata ggtggttcaa
     1561 ctcgaatgcc aatggtacaa agaaaattgg aagagaaaat tgggaagaag attgtatatt
     1621 ctattaatcc agatgaagct gtagcacaag gagcagctat tcaagcagca ttggaaatcg
     1681 ctaataaatc agaaagtgca gatgttagtg aaacagttcg tggattagca gaaaagttag
     1741 ttgtttcgga tgtcacttct caagccttgg gaactctggc tctttcgaat ggtgtgaaaa
     1801 gaaatacaat tattattcct aaaaattcca aggtaccaaa caagtactct gagtattttt
     1861 caacagttgt agataatcaa caaaatgtgt tagtagaggt tactcaaggg gatgacgagg
     1921 atccgcaatt tgttacggtt ataggagaga gtaccattaa attaccaggt gactggcctg
     1981 caggaacggt tttgaaggta acttatcatt atgatgtcga tcaaacagtt tttgtagagg
     2041 tacatgattc ggataatcac ttattaggga cttttgaagt agagagacaa gcaaatctcg
     2101 ataaaagtgc tgtagaagta gctagtcgta aaataaaaga tttaactatt gattagggga
     2161 ggaacacaat gtcggtcata aactattatg aagagttagg gatttcagaa acaagttcat
     2221 tagatgatgt taagaaaagt atcaagtcaa atagaagacg ctatcgccag ttgacaggtt
     2281 caccaaatat tgatcaaaga tcaatggctg agagaaagat ggaagtcatt gcacaggcag
     2341 aaaaagtatt tgaaagtgaa gaaaccagac aaaaatatga tagggaatta gaaaattcta
     2401 aacaaagttc tgaaggagtc cctgattcga ctccaactaa tcattcaaat tcaagctatc
     2461 ttgattcagc tagacaggcc ttttattcag gaaagaaatc attagcttat tcctacattg
     2521 aagaagcctt aaaaataaat cgaaatgatg cagatgtttg gtatttcaag gctatgatta
     2581 gccttgaaga tagaaagtta tcagatgcag aattagctat tagtgaagct aatagattga
     2641 gacctaaaaa tgcggatatc ttatctttgc taggtgatgt ttattgtgaa cagaatcagc
     2701 aaaagtttgc aattcagtat tatcaggaag cttttgaact atctaataat tccttttatc
     2761 ttttgaaaaa gggaagatca ctatttttat ttgatcagta taaacaggca gtaaaagatg
     2821 tgcgatatct gtatgatcat tatttagaag aaaataaaaa taatgaagaa tatatttatt
     2881 accttttatc atgctcttat agagaaattg gagataatga agaagcctat aattttgcaa
     2941 gaaaattagt agatgtttca gattcactag agcataaact acattatgca ggtattctat
     3001 ttttgaaatc tgaagatgag tgtgagaatt atttagatac cttaaacgaa cgctatggta
     3061 acaataaaga tttccagcaa gtgtatttta agttactatt agatatcacg atagagcgta
     3121 tgaaaaaagc acctgataca gacgttgagg ctttctttag aaaatatatt gatcgaacaa
     3181 aatatgagaa tgaagcagtt ctagataata aggcgcgtgt tcgcattgca gaagcttact
     3241 tggccaaggc acaaagttta gggattgaaa ataaaattat agataatgat ttttacaatg
     3301 aatttattga aaataaaaat tatatcaatt atgccacaac acgccattca caaggtatgg
     3361 gatgtctcat gttcatctta gtttttgcag ccaattggtt tttgagtggt ttctttgata
     3421 agtttgtaca tagcttttgg attagtaacg cattaagttt ggcattgtta tcaaatattg
     3481 tcttgtatta caattaccct aaaggatggc aaataaatag aagaaagaaa ggataaagtt
     3541 tttatgagtg aagaagtatt gcatgaaaaa gtttcgtata tcgtagataa agttgaagat
     3601 atcgaatctt taattacacg ccgtttattg gaagataaag ccaaaaatag tctgattgaa
     3661 gaattgaagc agtatttaat ctatagacag gatttagata agggtgagaa atttgctcca
     3721 tttatgaaac agattcttca agttattgat cgtattgaat catctgaaga aaaatcagat
     3781 ttgcttactt caattgctga agagcttctg caaattcttt cattgaatgg attacaagtt
     3841 attgataata gtggaatgat tgacccaagt atgcatgagg ttgttaatac agtagcagtt
     3901 actgatgaac aaagtgaaaa taatattgtt gaagtattgc aaaaaggata tttgttaaat
     3961 aacagggttc ttagaccaag taaagtgact attgctaaat aagatatttt ttaggaggtg
     4021 gagaaattgt tgcgcttttt taagcatcgc agaaacatac tatattttat attaggattt
     4081 ctttggggca gaagacaaaa tgctaaggtt tctccagaac cacctactcc ttccacacct
     4141 aaacatagtg aacttcccag tatttccaag gcaactcata atggtaaaat gacgggattt
     4201 gaaccacaaa aacttaagac ttatcaactt taccagcatg aattgatgtt tggagaacct
     4261 ggaaaaggat tgaataaatc aggattcgat gaatctgctg ttaatcttgg tcaagagggt
     4321 gaaatcaatt ttgctaaagc attacaaaaa aaagggcttt tagaaaaact agttaccttt
     4381 tggtctgttc ataatctcaa tttagaagat gaaagggtag atgcggatat tgattgtgtt
     4441 attgtttctg gatctacaat ttggttagta gatttaaaat tttatgcttc aggaaatgtg
     4501 atttatagag aagcggatgg tttattgtac actatagata gtgctacagg tgctcaaata
     4561 ggcagaccta aaaaaatgtc ccctaacatg tcttatgcag aagaaagctt ttcccataaa
     4621 tttgcgagtt tattaaaata ttatcgcttg gaaacacgtg ttgtcttaat gcctacctat
     4681 aagggtgcag gtagattgga taatgtcttt tggccaggtc atattaaggc tgttagtcta
     4741 gaagagatgt tagatgagtt atctagggaa gataagttta gggatacaat tggtgggcag
     4801 atgattcggc agacatttaa tttacttttg aaacgttaaa ttatgtaaaa atagtaaaga
     4861 attgatgttg tagatttttt atatgagtta tattttaaga ataatatgag ccgaattgaa
     4921 tttactcgaa cttatacaat
//
LOCUS       DAVKLX010000080         4899 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKLX010000080 DAVKLX010000000
VERSION     DAVKLX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.080"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            89..685
                     /locus_tag="VW325_002017"
                     /pseudo
     CDS             89..685
                     /locus_tag="VW325_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            761..1210
                     /locus_tag="VW325_002018"
     CDS             761..1210
                     /locus_tag="VW325_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9735340.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVGLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            1223..2298
                     /locus_tag="VW325_002019"
                     /pseudo
     CDS             1223..2298
                     /locus_tag="VW325_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2288..3933
                     /locus_tag="VW325_002020"
                     /pseudo
     CDS             2288..3933
                     /locus_tag="VW325_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4036..4728)
                     /locus_tag="VW325_002021"
     CDS             complement(4036..4728)
                     /locus_tag="VW325_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9735341.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1548 a         1036 c          843 g         1472 t
ORIGIN      
        1 aaagtgtact gttatagatt caatatacta tagaatagaa ttgtaggttt taaaataaac
       61 ttaaataaca caacggagat atcccatcat gaaaaagaaa tggatgtatt atgctgcttg
      121 ttcttctaat gaatctgccg atgacagttc atctgataaa ggagacggtg gttcgctagt
      181 cgtttattga ccaaactcag aggacttaat tggagcaact attcctgcct ttgaagaaaa
      241 atatggtatc aaagtagaac tgattcaagc tggtactgga gaacttttca aaaaactaga
      301 gtcagaaaaa gaagttcctg tagctgatgt tatctttggt ggttcttata cacaatatgc
      361 tacccacgga gaactctttg aaaactatac ttcaaaagaa aatgataatg ttatcaaaga
      421 atatcaaaac acaactggct actctactcc ttatacacta gatggtagtg ttttaatcgt
      481 caaccctgat ttaactaaag gcatgaacat cgaaggatat aacgatcttc tcaaacctga
      541 actaaaagga aaaatcgcaa ctgccgaccc agcaaactct tctagcgcct ttgctcaatt
      601 aacaaatatg ctacaagctc aaggtggtta caaagatgat aaggcttggt cttatgtaaa
      661 agatcttttc acacttattg atggtatagt aaaatgaaat aagaacagga caaatcaatc
      721 aggacagtca aatcgatttc taacaatatt ttagaagtag atgtgtacta ttctagtttc
      781 aatatactat aaaatcggtt taagttcatc tggtgtctat aaagcagtcg ctgatggaga
      841 aatgactgtt ggtctctctt atgaagatcc agcagttaaa ctcttaaatg acggagctaa
      901 cattaaggta gtctatccaa aagaaggaac cgtcttccta cctgctagtg ctgctatcgt
      961 taaaaaatct aaaaatatgg aaaatgccaa gaaatttatc gattttatta tctctcaaga
     1021 agtacaagat acacttggta caaccactac taaccgtcct gttcgtaaaa atgctaaaac
     1081 aagcgaaaac atgaaaccaa ttgacaaaat caaaacactc actgaagatt atgattatgt
     1141 catcaagaat aaatcagata tcgttaagaa atacaacgaa gtctttacag atatccaatc
     1201 taaacagtaa aagaggttca ctatgagtga gatcaaaatt attaacgcca aaaaaatcta
     1261 ccacgatgtc cctgttattg agaatttgaa cattacaatt ccaaaattct tggagcttca
     1321 ggatgtggga aaacgaccct tcttcgtatg attgcaggtt tcaacagtat caaagatgga
     1381 gaattttact tcgatgatac aaaaatcaat aatatggaac ccagcaaacg caatatcggg
     1441 atggttttcc aaaactacgc tattttccca catttgactg tccgagacaa cgttgctttt
     1501 ggtcttatgc aaaagaaggt tccaaaagaa gaattgattc aacagaccaa caagtatctt
     1561 gaactcatac aaattgctca atatgcggat cgaaagcccg ataaactcag tggtggacaa
     1621 caacaacgtg tcaccttggc atgcgcctta gcggttaatc caagtgttct cgtcatggac
     1681 gagccactta gtaatctgga ggccaaactt cgcttggata tacgtcaagc catccgagaa
     1741 atccaacacg aagtgggaat tacaactgtt tatgtaaccc acgaccaaga agaagccatg
     1801 gctatttcag accaaattgc tgttatgaaa gatggggtga tccaacaaat cggccgacca
     1861 aaagaactct atcataaacc agctaatgag tttgtggcaa cctttatcgg acgcacaaat
     1921 attatccctg ccaatcttga aaaacggagc gacggcgctt atatcgtctt ttcagatggc
     1981 tatgctcttc gaatgctagc tcttgatcag gttgaggagc aagctattca tgtaagcatt
     2041 cgtcccgaag agtttatcaa agatgaatct ggagatattg aaggaactat tagcgatagc
     2101 gtctatcttg gactaaatac ggagtatttc attgagacag gttttgcctc aaaaattcaa
     2161 gttagtgaag aatcaacttt tgaagaagat ctacaaaaag gcaatcgtat tcgtctacga
     2221 atcaatacgc aaaaattaaa cagcttttct gcagatggtt cccaaaatct gataaaagga
     2281 gtcaaccatg gaatgtaaaa aactaaatat ttggacagct tcctctttct tcctctttct
     2341 tacctatcct atctttctcg tttatcctat cgttaccgtg ctcaagcaag cacttataca
     2401 tgaaggacaa ttctcactag ctaattttgt cactttcttt agtaaagcct actactctga
     2461 gacactagtc aacagtttca aggtttccat taccgctact atcacttcct tagttgtagg
     2521 aaccctatta gcttatctct tctctatgta tgacttcaag gggaagaaat ttctacaaat
     2581 attgattatc attgcttcca tgtcagctcc tttcgtagga gcctactcct gaattctctt
     2641 gctgggacga aatgaggtca tcactaaatt tttgacaaat gcccttcatc ttccagctat
     2701 cgatatttat ggattcaaag gaattgtact tgtctttaca ctgcaactat tcccactggt
     2761 atttctatat gttgctggaa caatgaacag tattgacaat tctctacttg aagcagctga
     2821 aagcatgggg tccttcggat ttaagcgtat cgtaacggtt gttttacctc tcctagttcc
     2881 aaccttacta gcagatcctt gcttgtattt atgagagcat tctcagactt tggaacgcct
     2941 atgttgattg gcgaaggata tcggactttc cctgtcctga tttataccca atttattagc
     3001 gaggttggag gaaattctgc ttttgcaatt atggcgatta tcattgcctt ggcaattttc
     3061 cttatccaaa aacacattgc aaaccgctac agtttcagca tgaatctgct ccatccaatt
     3121 gagcctaaaa aaactacaaa aggaaaaata gctgccattt atgcaacagt ctacggaatt
     3181 atctttatct ctgttttacc tcaaatctac ttaatttata cctctttcct aaaaacatca
     3241 ggtatggtat ctgttaaagg ttattctcca aacagttaca aggtagcttt caatcgtatg
     3301 ggatctgcta ttttcaatac cattcgtatc cctttgattg ccttagttct agttgttcta
     3361 tttgcgacat ttatctccta cctagccgtt agaaaacgga atttgtttac aaacttaatt
     3421 gacagcctca gtatggtacc ttatattgta ccaggaaccg ttctagggat tgccttcatt
     3481 tcttccttca atactggtct atttggaagt ggatttctta tgattacagg gactgctttc
     3541 atcttgatta tgtctctatc tgtcagaaga ttaccgtata ctattcgctc atctgttgct
     3601 agcttacaac aaatagcacc aagtattgaa gaagctgctg aaagcttagg aagtagtcgt
     3661 ctcaatacct ttgctaagat tacaactcca atgatgctat ctggtatcat ttctggagcc
     3721 atcttatctt gggtcacaat gatttcaaaa ctctctactt ctatcctcct ctacaatgtc
     3781 aaaacaagaa caatgactgt agctatttat acagaggttc tcagaggaaa ttacggtgta
     3841 gccgcagcct tgtcaactat cctgactgtt ctaacagtag gttccttgct cttgtttatg
     3901 aaaatctcta aaagcaatag cattacactt tagttttcca catcaaaaac agccgaaagg
     3961 agtaccctcg gctgtttttt cttatcttga tattcttatc aaatccctag ctcatggcaa
     4021 gcaagtctag actgattaaa tagagccttc ttccatcttc tcacgtccta gctctcggta
     4081 actacggtca ccgacaactt caaccctaaa ctgaccgtcc tcatattcaa gaatcgtcac
     4141 gctaccatta tctagaccat gcggatgcat gccattaatc agataaacaa tggttccaat
     4201 agtcattcca tggctgacaa caagggcgtt acctccacct tgatcttcca tttcttttgc
     4261 aatcatttca aagccttcct tgattcggcc actgagtttt tcccagcctt cagcccaacc
     4321 agctgtatcg acctctacca agccctcagc cagttcagca taagacaatt ggtgaacgtg
     4381 gtccacatta aagatacgag gaataatgcc catgaaaaga tcgccatcat aggctccatc
     4441 aaaactaccg aaacaccatt ctctgatacg cttgtccatg cgataaggga ttttcccctg
     4501 caagccaagt tcttcaagga taattcccat ggtctgaatg gtacgaccag aatcactcga
     4561 ataagcgcgc tcaaactgta gatcagattc tcgcaaaccg attcctaact cttgaatccc
     4621 tcgttcacct tcagcagtta agggagtatc gctccaacct tgcgcgcgac caatcgtgtt
     4681 aaacatggtc ttgccatgac gtaccaaata caatcgtact tttaccattt tccgtcctcc
     4741 gtttgcttct attatatcat gaatgataaa ataaaagcca cccatacagg cgacttttga
     4801 aggagattat tatgaaaaag tttaggagtt cattaaataa agatattaga tgaaaatcaa
     4861 attcaaacta attcagtgtt atctgtttca aataatatt
//
LOCUS       DAVKLX010000081         4738 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKLX010000081 DAVKLX010000000
VERSION     DAVKLX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.081"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            28..1573
                     /locus_tag="VW325_002022"
     rRNA            28..1573
                     /locus_tag="VW325_002022"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1625..1697
                     /locus_tag="VW325_002023"
     tRNA            1625..1697
                     /locus_tag="VW325_002023"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1658..1660,aa:Ala,seq:tgc)
     gene            1822..4723
                     /locus_tag="VW325_002024"
     rRNA            1822..4723
                     /locus_tag="VW325_002024"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1313 a          973 c         1410 g         1042 t
ORIGIN      
        1 tgacagaaat gagtgagaac tcaaactttt aatgagagtt tgatcctggc tcaggacgaa
       61 cgctggcggc gtgcctaata catgcaagta gaacgctgaa ggaggagctt gcttctctgg
      121 atgagttgcg aacgggtgag taacgcgtag gtaacctgcc tggtagcggg ggataactat
      181 tggaaacgat agctaatacc gcataagagt ggatgttgca tgacatttgc ttaaaaggtg
      241 cacttgcatc actaccagat ggacctgcgt tgtattagct agttggtggg gtaacggctc
      301 accaaggcga cgatacatag ccgacctgag agggtgatcg gccacactgg gactgagaca
      361 cggcccagac tcctacggga ggcagcagta gggaatcttc ggcaatggac ggaagtctga
      421 ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt aaagctctgt tgtaagagaa
      481 gaacgagtgt gagagtggaa agttcacact gtgacggtat cttaccagaa agggacggct
      541 aactacgtgc cagcagccgc ggtaatacgt aggtcccgag cgttgtccgg atttattggg
      601 cgtaaagcga gcgcaggcgg ttagataagt ctgaagttaa aggctgtggc ttaaccatag
      661 taggctttgg aaactgttta acttgagtgc aagaggggag agtggaattc catgtgtagc
      721 ggtgaaatgc gtagatatat ggaggaacac cggtggcgaa agcggctctc tggcttgtaa
      781 ctgacgctga ggctcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg
      841 ctgtaaacga tgagtgctag gtgttagacc ctttccgggg tttagtgccg tagctaacgc
      901 attaagcact ccgcctgggg agtacgaccg caaggttgaa actcaaagga attgacgggg
      961 gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa ccttaccagg
     1021 tcttgacatc cctctgaccg ctctagagat agagttttcc ttcgggacag aggtgacagg
     1081 tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg
     1141 caacccctat tgttagttgc catcatttag ttgggcactc tagcgagact gccggtaata
     1201 aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct gggctacaca
     1261 cgtgctacaa tggctggtac aacgagtcgc aagccggtga cggcaagcta atctcttaaa
     1321 gccagtctca gttcggattg taggctgcaa ctcgcctaca tgaagtcgga atcgctagta
     1381 atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
     1441 accacgagag tttgtaacac ccgaagtcgg tgaggtaacc gtaaggagcc agccgcctaa
     1501 ggtgggatag atgattgggg tgaagtcgta acaaggtagc cgtatcggaa ggtgcggctg
     1561 gatcacctcc tttctaagga taaggaactg cgcattggtc ttgtttagtc ttgagaggtc
     1621 ttgtggggcc ttagctcagc tgggagagcg cctgctttgc acgcaggagg tcagcggttc
     1681 gatcccgcta ggctccattg gtgagagatc accaagtaat gcacattgaa aattgaatat
     1741 ctatatcaaa tagtaacaag aaaataaacc gaaaacgctg tagtattaat aagagtttat
     1801 gactgaaagg tcagaaaata aggttaagtt aataagggcg cacggtggat gccttggcac
     1861 taggagccga cgaaggacgt gacaaacgac gatatgcctt gggtagctgt aagtaagcga
     1921 tgatccaggg atttccgaat gggggaaccc aacaggtaat acctgttacc cacatctgtt
     1981 aaggatgtga ggaggaagac gcagtgaact gaaacatcta agtagctgca ggaagagaaa
     2041 gcaaaagcga ttgccttagt agcggcgagc gaaacggcag aagggcaaac cgaagagttt
     2101 actcttcggg gttgtaggac tgcaatgtgg actcaaagat tatagaagaa tgatttggga
     2161 agatcagcca aagagagtaa tagcctcgta tttaaaatag tctttgtacc tagcagtatc
     2221 ctgagtacgg cgggacacgt gaaatcccgt cggaatctgg gaggaccatc tcccaaccct
     2281 aaatactccc tagtgaccga tagtgaacca gtaccgtgag ggaaaggtga aaagcacccc
     2341 gggaggggag tgaaatagaa cctgaaaccg tgtgcctaca acaagttcga gcccgttaat
     2401 gggtgagagc gtgccttttg tagaatgaac cggcgagtta cgttatgatg cgaggttaag
     2461 ttgaagagac ggagccgtag ggaaaccgag tctgaatagg gcgccttagt atcatgacgt
     2521 agacccgaaa ccatgtgacc tacccatgag caggttgaag gtgcggtaag acgcactgga
     2581 ggaccgaacc agggcacgtt gaaaagtgct tggatgactt gtgggtagcg gagaaattcc
     2641 aaacgaactt ggagatagct ggttctctcc gaaatagctt tagggctagc gtcgacatta
     2701 gagattcttg gaggtagagc actgtttggg tgaggggtcc atcccggatt accaatctca
     2761 gataaactcc gaatgccaat gaattatggt cggcagtcag actgcgagtg ctaagatccg
     2821 tagtcgaaag ggaaacagcc cagaccacca gctaaggtcc caaaataatt gttaagtgga
     2881 aaaggatgtg gggttgcaca gacaactagg atgttagctt agaagcagct attcattcaa
     2941 agagtgcgta atagctcact agtcgagtga ccctgcgccg aaaatgtacc ggggctaaaa
     3001 caatttaccg aagctgtgga tacctttata ggtatggtag gagagcgttc tatgtgtgat
     3061 gaaggtatac cgtgaggagt gctggaacgc atagaagtga gaatgccggt atgagtagcg
     3121 aaagacaggt gagaatcctg tccaccgtaa gactaaggtt tccaggggaa ggctcgtccg
     3181 ccctgggtta gtcgggacct aaggagagac cgaaaggtgt atccgatgga caacaggttg
     3241 atattcctgt actagagtat gtagtgatgg agggacgcag taggctaact aaagcagacg
     3301 attggaagag tctgtctaag cagtgaggtg tgaattgagt caaatgctta attctataac
     3361 attgagctgt gatggggagc gaagtttagt agcgaagtta gtgacgtcac actgccaaga
     3421 aaagcttcta gcgtttaaac atactctacc cgtaccgcaa accgacacag gtagtcgagg
     3481 cgagtagcct caggtgagcg agagaactct cgttaaggaa ctcggcaaaa tgaccccgta
     3541 acttcgggag aaggggtgct gacttaaagt cagccgcagt gaataggccc aagcaactgt
     3601 ttatcaaaaa cacagctctc tgctaaatcg taagatgatg tatagggggt gacgcctgcc
     3661 cggtgctgga aggttaagag gagtgcttag cgtaagcgaa ggtatgaatt gaagccccag
     3721 taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc
     3781 cgacccgcac gaaaggcgta atgatttggg cactgtctca acgagagact cggtgaaatt
     3841 ttagtacctg tgaagatgca ggttacccgc gacaggacgg aaagacccca tggagcttta
     3901 ctgcagtttg atattgagtg tctgtaccac atgtacagga taggtaggag tctaagagat
     3961 cgggacgcca gtttcgaagg agacgctgtt gggatactac ccttgtgtta tggccactct
     4021 aacccagata ggtgatccct atcggagaca gtgtctgacg ggcagtttga ctggggcggt
     4081 cgcctcctaa aaggtaacgg aggcgcccaa aggttccctc agaatggttg gaaatcattc
     4141 gcagagtgta aaggtataag ggagcttgac tgcgagagct acaactcgag cagggacgaa
     4201 agtcgggctt agtgatccgg tggttccgta tggaagggcc atcgctcaac ggataaaagc
     4261 taccctgggg ataacaggct tatctccccc aagagttcac atcgacgggg aggtttggca
     4321 cctcgatgtc ggctcgtcgc atcctggggc tgtagtcggt cccaagggtt gggctgttcg
     4381 cccattaaag cggcacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
     4441 gtcgcgggcg taggaaattt gagaggatct gctcctagta cgagaggacc agagtggact
     4501 taccgctggt gtaccagttg tcttgccaaa ggcatcgctg ggtagctatg tagggaaggg
     4561 ataaacgctg aaagcatcta agtgtgaaac ccacctcaag atgagatttc ccatgattat
     4621 atatcagtaa gagccctgag agatgatcag gtagataggt tagaagtgga agtgtggcga
     4681 cacatgtagc ggactaatac taatagctcg aggacttatc caaagtaact gagaatat
//
LOCUS       DAVKLX010000082         4342 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKLX010000082 DAVKLX010000000
VERSION     DAVKLX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.082"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            99..173
                     /locus_tag="VW325_002025"
                     /pseudo
     CDS             99..173
                     /locus_tag="VW325_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(635..796)
                     /locus_tag="VW325_002026"
     CDS             complement(635..796)
                     /locus_tag="VW325_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9735342.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNP"
     gene            complement(781..999)
                     /locus_tag="VW325_002027"
     CDS             complement(781..999)
                     /locus_tag="VW325_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9735343.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            complement(1097..1882)
                     /locus_tag="VW325_002028"
     CDS             complement(1097..1882)
                     /locus_tag="VW325_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9735344.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            complement(1884..2702)
                     /locus_tag="VW325_002029"
     CDS             complement(1884..2702)
                     /locus_tag="VW325_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9735345.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            complement(2704..3696)
                     /locus_tag="VW325_002030"
     CDS             complement(2704..3696)
                     /locus_tag="VW325_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9735346.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
BASE COUNT         1465 a          929 c          749 g         1199 t
ORIGIN      
        1 ataaaaacag tatatcattt aaaaaaattt aattcaagaa aaattaacat gcattaattt
       61 attttttagc acacgttaaa aaataagatt agtactcaat gaaaatcaaa gagcaaacta
      121 ggaaactagc cgcaggttgc tcaaaacact gttttgaggt tgtagatgga actgacgtag
      181 tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagt aaccatacgc
      241 acggcaaggc gacgttgacg tggtttgaag agattttcta tgagtataag tccaaaactt
      301 tcgttaaccg ggatacatta taagcacatt cataccatca aaaatagtag caataaactt
      361 gaacaactac cagaaacacg aaaacaggca taattgatga aatatcagcg ctattaaaca
      421 atataaataa ccgctaagaa taaggcttaa cggttatctt ctacctatct attttttcaa
      481 ctcctcagtc gtttatctac ctataaccta ggctttacga gtgccttgct ctaccaactg
      541 agctaaatcg gcgattacac ttacagtatg gcactctgag aatagatgtc aagtaatatt
      601 attcaagctt tttaatcaaa atctgacaag gattttacgg attccatagc tgagaaaaaa
      661 tctctaactt tttaaagcca agacctcgat aaaagtcatt tgttcgatca taatctttat
      721 tagaaccttc tgccactgtt ttgacctgca gataaccaac ttttttacga gcttcactct
      781 ctaaagtagc aagcaattgg ctcccaattt ttctaccttg ataagctttt ttacgccgag
      841 acaatcaatc tctgcacaat cttcactcga ataggataac cttacaaatc cagtcaaatc
      901 actctcctga taggcggtcc aaacttgcag tgtcgtggtt ccttctatat aggcttgtgt
      961 gctttctggg attccaaacc attctggcaa atccttcaaa acctcaacga caactgcctt
     1021 ttttgatttt catccttgac ttcttcaatt acaaacatca taagttcctt tcttgatttt
     1081 agtcttactt tagcttttac gaacccgcac tttcgtagga atctaagccc ttatcccaaa
     1141 gtttaaggga aataacaaag aaaaccagag aaatcaacat caaacctcct acgttaaaga
     1201 acacatcctt ttcctgtaag aaatagctag ctggatagta ggctgtaaag gcgaaaggca
     1261 cgataaagct aatcaaccaa cgaagaagag aattgtaaat agaaatcgga tacttagcaa
     1321 agtcattgaa catatagaag atgtaaatca tggcgcctga ctgcttagtc caaaaggcga
     1381 tactggctgt tgcgatttta agagaagtat aaatcaaggt cgcaaaagga atacaaacta
     1441 ggaaaagcag gaattttgga agagtccaaa caatgctggt cactgttgtt cccaataaaa
     1501 taccaccgac taagagttca cccaaggcat caatctgaaa ggtttcaact aggatgtgaa
     1561 agagaggatt gatgggacga gtcagatact tgtcaaactc tccttttcgg actaggcgtt
     1621 gccctagtgc ccagagattg tcaaaaaaga gatggtccat tcccttggga atcaaggaaa
     1681 atccataaat gaaagctatc tcttgaaagg tccagccttc taggaatgga atatgttgaa
     1741 agatgacatt gagaaacaag agattcaagc cttgagccag aaagactccc aagacaccaa
     1801 ccacaaaatc caccttatat tccatgattt gtttgatgta ttgtctgata aaaatcagat
     1861 gcattcgttg atattttttc atactaacct ccttgaatgg tgataaagga ctggacccgt
     1921 ttccaaatta actgagacaa tcccaccatc actaagagcc agaagaactg caaaaggagt
     1981 gcctgaagaa tctgactggc atcgtatttt ccaacaatga tcataactgg agtataaatc
     2041 aaggacgaaa aaggcaaaaa ggagagaata tctgaaacaa cctttggaaa gaatgccaag
     2101 ggaatcaaac tccccgacat aaaagccact atggaagtct taagtaggtt ggaaccccaa
     2161 agatttttaa acacaaaggc tgaaaatcca aagcaaatat taaagaaaaa gttaatcaga
     2221 taggcgagcg ttaagctaaa aagataaagg acagttaatc ctagcacctc tacaatacct
     2281 tgacccgata tgattttcat caagacaatg acacttaaaa atggaaggcc aacgctgata
     2341 aaaatcaacc acttggaacc aagctcggtg aaaagatagg aggccgcaaa atgcactggt
     2401 cgcaacaaac gcatgataat ggagccatcc ttgacctcct ccccaatcat aaaggacgaa
     2461 tcggatctag tcagaagatt ggtcacaaaa ctcatgatga tgtagagggt gatatccgcc
     2521 atactgaagc cctgaatcaa agactcttgc gaagaatcaa agacagcctt ccagagataa
     2581 aaggccacaa aagcccccat gacatcgcca atccgataga gaataaagtt gactcggtaa
     2641 gtaatcaact cctgaacccc tgcattgata aagggtttat aacgtctcca caatttgatc
     2701 atcctagagc tcctttcggt agaagcgacg gataatatcc tcaatatccg tatccaccat
     2761 cttcaaatcg cggatttcaa aatcagatag ggtttgcttg ataatgtcag ctgactggta
     2821 gcgagaacta tcaaattcaa tgttgaggct gtttccttgt ctatcaatgg tcatatcaga
     2881 cagaccttca tagtgagaga cgagatgact ttgacctggt agcagttcaa aagagagagt
     2941 cttcatctta ccaaaggtct ccttgagttg gctcaccgtt ccatcaaaaa tcttttgccc
     3001 cttgtcaatc atgaaaatcc gatcacaaag ttgctcaata tcactcaaat cgtgagtggt
     3061 caaaagaatg gtagtttctt cctcttgatt gatctgagta attgcccgac gaatattatc
     3121 cttaaccgaa acgtccaaac caatggtcgg ctcatctaaa aaaagaacct tgggattgtg
     3181 gagcaaggag gccgcaatat ccgctcgcat ccgttgtccc agtgaaagag tccgcacggg
     3241 atccttgata aagtccttca aatccaagac ttcattcaaa aagtccatac gcttatgaaa
     3301 gagcgagttt ggcacatcat aaatctcttt taagacagtg taggtctctt gcagagccaa
     3361 atcccaccat agctgggtgc gttgtccaaa gactacgcca atatctttga cataatcttg
     3421 ccgattgtcc tggggaatct tgccgttaat ccgacaaaaa ccagatgttg gtttcaaaat
     3481 tcctgtcagc attttaatgg ttgtcgactt cccagcacca tttgccccga taaatcctaa
     3541 aatctgccct tttggaacct caaaggtcaa atccttgacc gcttcaaagg tctgcttttc
     3601 aggatgaata aaggagcgca aagccccctt caagcccggt tccttaacaa tcttcacaaa
     3661 atgtttctga agatgttcca cttctatcat tgccatatct atctccctat cgtaaggaat
     3721 agcaacattg tgtttttaac tacaaatatt ctaaatcctt actttctaca ccttctacat
     3781 tttattacta cagaaggctt tataccaacc tattatagtc caataaaaaa cgaaatgcaa
     3841 gcgtttgcct atagattgtg aaattaggat ttctctcatg aaataatact tttaatcaaa
     3901 aattctgttt aatagtttaa attaccaacc tactcatctt ctcaccagag ggcggtatta
     3961 actttgatca taaaatcgga tactccctct atccagaaaa tatgctcaga atgattgtct
     4021 ctaaacaata cctgctcttt tacctcattc atgaaaaaga agtccatacc ctgagaatta
     4081 tcaactcacg caccgactac ctaaaccaac tcgaccacct ctttcgtaca tgtaggaaac
     4141 tattttcctc acaaataatc tcattataaa atccaataaa tttttttagc tcacttgaat
     4201 attttctttg tatcgtttta tcagcttcaa tctgatctag acttgttacc ttggaacgac
     4261 ttgaaataac ctttgtaaaa taacacttaa ggctttcatc atctcgtata aatttttcaa
     4321 tagtgatata aaaagctttc at
//
LOCUS       DAVKLX010000083         4321 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKLX010000083 DAVKLX010000000
VERSION     DAVKLX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.083"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            50..1039
                     /gene="manX"
                     /locus_tag="VW325_002031"
     CDS             50..1039
                     /gene="manX"
                     /locus_tag="VW325_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9735347.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYTKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            1067..1870
                     /locus_tag="VW325_002032"
     CDS             1067..1870
                     /locus_tag="VW325_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9735348.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            1894..2805
                     /locus_tag="VW325_002033"
     CDS             1894..2805
                     /locus_tag="VW325_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9735349.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2861..4195)
                     /gene="pepC"
                     /locus_tag="VW325_002034"
     CDS             complement(2861..4195)
                     /gene="pepC"
                     /locus_tag="VW325_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9735350.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
BASE COUNT         1180 a          930 c          910 g         1301 t
ORIGIN      
        1 taaagttttc ttaaatgaaa actaaaacaa aaaaggagga atgacaataa tgagtatcgg
       61 aatcattatt gcgagccacg gcgaatttgc tgcgggtatt catcagtcag gatctatgat
      121 ctttggtgaa caagaaaagg ttcaagttgt gacctttatg ccaaatgaag gtcctgatga
      181 tctatacact aagtttaata acgctgttgc tgcatttgac gcagaagatg aggttctagt
      241 tttggctgac ctttggagtg gttctccatt taaccaagct agtcgcgtga tgggagaaaa
      301 tcctgagcgt aagtttgcca tcatcacagg acttaactta ccgatgttga ttcaagccta
      361 cacagagcgc ctcatggacg ctgctgcagg tgtagaaaaa gtcgctgcta atatcattaa
      421 agaagccaaa gatggcatca aagctcttcc agaagagcta aatccagttg aagaagttgc
      481 aagcgctgca gctgctccag ttgcccaaac tgctatccca gaaggaactg ttatcggaga
      541 cggtaaattg aaaatcaatc ttgcccgtct tgacacacgt ctacttcacg gtcaggttgc
      601 aactgcttgg actccagatt caaaagcaaa tcgtatcatc gttgcttcag ataacgtggc
      661 taaagacgac cttcgtaaag aattgattaa acaagcagct ccaggtaatg tcaaggctaa
      721 cgtggttcca attcaaaaac tgattgagat ttcaaaagac ccacgttttg gagaaacaca
      781 tgcccttatc ttgtttgaaa cacctcaaga tgcccttcgt gccatcgaag gcggcgtgcc
      841 aatcaagact cttaacgttg gttctatggc tcactcaaca ggtaaaacat tggtcaatac
      901 cgttttgtct atggacaaag aagatgttgc tacatttgaa aaaatgcgtg acttgggtgt
      961 tgaatttgat gtccgtaaag taccaaatga ttctaaaaaa gatttgtttg acttgattaa
     1021 caaagccaat gtcaaataag ccattattta tgaaaggatt ttaaacatgt ctattatttc
     1081 tatggtttta gtagtcgttg tagccttctt tgcaggtctt gaaggcatcc tcgaccagtt
     1141 ccaatttcac caaccacttg tagcctgtac ccttattggg cttgtaacag gtcacttgga
     1201 agcagggatt atcctcggtg gatcgcttca aatgattgcc cttggttggt caaatatcgg
     1261 tgctgctatc gctcctgatg ctgcacttgc ttctgtcgct gctgccatta tcatggttct
     1321 tggtggtgac tttaccaaga ctggtatcgg tgttgcccaa gcggttgcta tccctcttgc
     1381 cgtagctgga cttttcttga caatgattgt tcgtacaatt tcagttggtt tggttcatac
     1441 tgcagatgct gccgctaaaa aaggtgactt cggcgctgtg gagcgtgcgc atttcattgc
     1501 gctactcttc caaggacttc gtatcgcgct tcctgcagct cttctcctta tggtaccaac
     1561 tgaaactgta caaagtatcc ttagtgccat gccagactgg ctcaaagatg gtatggctat
     1621 cggtggtggt atggtcgttg ccgttggtta cgccatggtt atcaacatga tggcaactcg
     1681 tgaagtatgg ccattcttcg ctcttggttt cgttctcgct gctgtgtcag atattactct
     1741 aatcggattc ggtgctatcg gtgttgctat cgctcttatc taccttcacc tttctaaaac
     1801 tggtggaaat ggtggcggag gagccgcaac ttctaacgac ccaatcggcg atatcctaga
     1861 agactactaa gataagaaag gactgaaaac atcatgactg aaaaacttca attaactaaa
     1921 tcagatcgta aaaaagtttg gtggcgttca accttcttac aagggtcttg gaactttgaa
     1981 cggatgcaaa acttgggctg ggcttataca ctcattccag ctatcaaaaa actctatact
     2041 aaaaaagaag atcaaatcgc tgctcttgag cgtcaccttg agttcttcaa cactcatcca
     2101 tacgtagctg ctccagtcat gggggttact cttgcgcttg aagaagaacg tgctaacggt
     2161 gtggaaatcg atgacgctgc tatccaaggg gttaaaatcg gtatgatggg acctcttgct
     2221 ggtatcggtg acccagtatt ctggtttaca gtacgcccaa tccttggatc tctcggtgct
     2281 tcacttgccc ttactggcaa tatcttgggg ccactcctct tctttgttgc atggaacttg
     2341 attcgtatgt cattcttgtg gtatgttcaa gagattggat acaaggctgg atcagaaatc
     2401 actaaagata tgtctggtgg tatccttcaa gatatcacta aaggagcttc tatccttggg
     2461 atgttcattc ttgctgtcct tgttcaacgc tgggtaaata ttaaatttgc tttcgatgtt
     2521 tctaaagttc aactagatga aaaggcttat atccattggg ataaattgcc agaagggtct
     2581 aaaggtatcc aagaagcatt cgcacaagta ggacaaggat tgtctcaaac tcctgaaaaa
     2641 gttactactt tccaacaaaa cttggatatg ttgattcctg gattatcagg actactcctt
     2701 actttacttt gcatgtactt acttaagaaa aaagtatctc caatcactat tatccttgcc
     2761 ctcttcgcag tgggtattgt ggcacatgtt cttcacatca tgtaatcaag caactaaaaa
     2821 ggaaccaggt tctaaaatct gattcctttt ttctatgctt ttatttagcc aaggctccca
     2881 ttggatccca tggtgcaagt acaattggtt ctgctccata ggcagcttgt tcttctgctg
     2941 tcagcaattc cttacgaaca acgatttggt atgtgtattc gtccatccaa gcgtctgagg
     3001 caacaaagta accatctgta ccgaccttgt ctccccatga gttttcaacc ttccacttgg
     3061 ttgatttacc attttcgtcc aagtcaacac ctgtcaagac catggcgtgg gtcatcaagc
     3121 tttcgctata gtccaaacgt ccagccttgt cttgagtaag tttaatgtcc atgcttgatt
     3181 caaagtcata aacatctgtc gcaaggattc cagccttacg gttactgagc tggccgacat
     3241 cagaaccaaa ccaaacagtc tcacctgctt gcatttgggc aatcgccaat tctttcaagc
     3301 gctccattgg aacgttgatg taacgaactg cacggctacc aaccacattc cccaacatct
     3361 caactgtgta agattttccg taaggtttat cagcagttgg agcattgata acagaaacgt
     3421 agtcttctaa aggaagattg acatatttct tgtagaactc ttgtggtgtg attccttttt
     3481 cacttttgta gttgttatct ttatcgcgat aagcaaagtc aaacttgcgt ggtggaagtc
     3541 ctaatgacat agcaagaaag ttaaagattt cttgcaagag gtcttctttc ttagcttgaa
     3601 cagtcacttg atctgcacca gaaacaagca aatcacgcaa gatttgagca tcttgacgaa
     3661 gcaatttatt aaggatcgca tttagctcac gactgctgct agatgaaaca gactcaggat
     3721 aaactgactt aggcacgaca ccgtattttt caaagaggga aacgaccata tcccattgac
     3781 caccatcttg ttgaggtgtt tggagtaaga agctaacctt gcggctagtc aattcttggt
     3841 ctgaagtcgc aatgacttgc tccaagaacc agtttgattt ctcatactta tcccagaaga
     3901 aagtgtgggc ttgtgacaac tcaaagttct ccaatttgta ttgcgagatg agtttgtggc
     3961 ggaaggtgtt gagagccgca aacatccagc aacgaccaga cgctttctgg ttagtgacct
     4021 tgtccttggt taaatccaat gagaaaacag gtgtgttgtc tacatggctt tggcgacgtt
     4081 ccagagctgc aaaaattccg ttgtggctgg cagcattttc aatcgcttgg tattttacat
     4141 ttgcttcata gttggcaaat agtttatcag taaatgattc ttgaatcgcg ttcatagatt
     4201 cctcctttta ttctacagtc tattataact cttttcacaa aggaagcaac taaaaaacat
     4261 aaaaggtaag gacattctcc ctcacctttt tcgaatttat actcttcgaa aatctcttca
     4321 a
//
LOCUS       DAVKLX010000084         4313 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKLX010000084 DAVKLX010000000
VERSION     DAVKLX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.084"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            15..1040
                     /locus_tag="VW325_002035"
     CDS             15..1040
                     /locus_tag="VW325_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9735351.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1030..2037
                     /locus_tag="VW325_002036"
     CDS             1030..2037
                     /locus_tag="VW325_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9735352.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2037..3044
                     /locus_tag="VW325_002037"
     CDS             2037..3044
                     /locus_tag="VW325_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9735353.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            3044..3838
                     /locus_tag="VW325_002038"
     CDS             3044..3838
                     /locus_tag="VW325_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9735354.1"
                     /translation="MKGLWSNDLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVMANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT         1559 a          631 c          735 g         1388 t
ORIGIN      
        1 tttaaggagt ttttatgaaa aacaaatttt ttctaatagc tattttagct atgtgtatag
       61 tttttagcgc ttgttcttct aattctgtta aaaatgaaga aaatacttct aaagagcatg
      121 cgcctgataa aatagtttta gatcatgctt tcggtcaaac tatattagat aaaaaacctg
      181 aaagagttgc aactattgct tggggaaatc atgatgtagc attagcttta ggaatagttc
      241 ctgttggatt ttcaaaagca aattacggtg taagtgctga taaaggagtt ttaccatgga
      301 cagaagaaaa aatcaaagaa ctaaatggta aagctaacct atttgacgat ttggatggac
      361 ttaactttga agcaatatca aattctaaac cagatgttat cttagcaggt tattctggta
      421 taactaaaga agattatgac actctatcaa aaattgctcc tgtagcagca tacaaatcta
      481 aaccttggca aactttatgg agagatatga ttaaaattga ttcaaaagcc ttaggtatgg
      541 aaaaagaagg tgatgagtta atcaaaaata ctgaagctcg tatatccaaa gaattagaaa
      601 aacatccaga aatcaaagga aaaatcaaag gaaaaaaagt attatttact atgattaatg
      661 ctgcagatac atcaaaattc tggatttata ctagcaaaga tccaagagca aattatttaa
      721 cagatttagg tctagttttc cctgaatcat taaaagaatt tgagagtgaa gatagttttg
      781 caaaggaaat ttctgcagaa gaagcaaata agataaatga tgctgatgta atcataactt
      841 atggtgatga taaaactctt gaagctttac aaaaagatcc tcttttaggt aaaataaatg
      901 caattaaaaa tggtgccgtt gctgtaattc cagataatac accgttagca gcctcatgca
      961 ctccaacacc actttcaata aactatacta ttgaagaata tctaaatctt ttaggaaatg
     1021 catgcaaaaa tgcgaaataa aaaacaaata aacctaggca taatttttgt aatctgccta
     1081 ggtcttctta ttacaatatt tttgtcatta aagcttggaa caaaagaaat taatatcaga
     1141 gattttttag cagcttttgg aatgggtaat acaaatgatg attttattaa atcaattata
     1201 tataaaagaa tacctagaac tatttttgca attttagcag gttctagtct tgccataagc
     1261 ggtgtattga tgcaatcagt tactagaaac ccaatagctg atccaggtat acttggtata
     1321 aacacaggag caagtcttag tgtagtaatt ggtctttctt ttttaggaat ttcatcaagc
     1381 ataagccata taagttttgc aatcattggt ggcttagtaa gtgcaatttt tgtatacgcg
     1441 attgctgtaa gcggaaaagc aggccttacc cctataaaac ttgccttatc aggaacttgt
     1501 gttagtatgg ctttaagcag ttttgtaagt tttttaattt taccgaataa taacgtctta
     1561 gacaaattta gattttggca aataggtagc cttggagcag ctacattatc ttctatatct
     1621 acactactac cttttataat tttaggtcac ttgatagcta tatttatttc atcagattta
     1681 aacgctttag ctatgggtga tgaaatggct gttggtcttg gagttaatgt taataggata
     1741 agatcacttg caataattgc aagtgtgctt ttatgttcaa gtattactgc aattggtgga
     1801 cctattggct tcgtaggtct tatagttcct cacttttgtg gcttatttat aagcaaagat
     1861 atacgcacaa tgaccatttc ttcatctttt ataggtgcag agctcttgct tatatgtgat
     1921 ataatcggcc gtatgttagg taaaccaggt gaaattgaag tagggataat tactgcaata
     1981 atcgggggtc cagtacttat ttatgtaact atgaaaaata gaggggttaa taactaatgc
     2041 aaaatttaat tataggtatt caaaaaagaa aaaatagaat aacactattc tcctcactat
     2101 ttttattaat aataatcagt ctatcatttt tcattttact tatcggagat gaaagttatt
     2161 ctttttcaac tttgattaaa gtcttaaata gtgaaactgt tcctggagct agtttttcga
     2221 ttatggaaat tagattacca aaattattag caggaattat agctggctgg tcttttggat
     2281 tggcaggatt tatctttcaa actatgttaa gaaatcctct tgcaagtcct gatataatcg
     2341 gtgtcacaag ttcttcatct attgcagcgg tcttttgcat attggtatta aaaacaaata
     2401 gtttaactac tggaattatt tcaataactt gtggactaac atcatcttta atattatttt
     2461 tactagctaa aaaagatggt ttttcagcag caagactgat aatattaggt attggttttc
     2521 aagctgtcac aagagcaggc acctcatttt tattgttgaa agtagcaaga tatgaattac
     2581 aagaagttat gagatggctc agtggctctt tatcttttac aaagttagat gacatacctc
     2641 ttgttctaat agtaagtatt attgctacta tattagtttt attttttaat aaaagactag
     2701 aaattattga acttggtgaa gaaatagcaa tcggacttgg agcaaatccc gagctttcaa
     2761 ggcttgtttt aattttttgc gctgtatctt taactgcttt ttctacttca attacaggac
     2821 caatagcttg tatatctttt ttagctggtc ccatagcctt aaatattggt aagaaaagaa
     2881 gcccaatatt agctggattg gttggaattt tactagtttt gttatcagac atattctctc
     2941 aaaatatttt accagctaga tatccagtag gtgttgtaac tggcttgtta ggttcaccat
     3001 acttaatata cttactaata aaaatgaaca ggagaaatat ataatgaaag gtctgtggtc
     3061 aaatgatctt acctgcggtt atgatgaaaa aataatcttg gaaaatataa atataaaaat
     3121 acctgaagaa aaaatatcag ttattattgg gtcaaatggt tgtgggaaat caacactcat
     3181 taaaaccttg tctcgactta taaagccatt agagggagaa gtattgcttg ataataaatc
     3241 aattaattct tataaagaaa aagatttagc aaaacacata gctatattac ctcaatctcc
     3301 aataatccct gaatcaataa cagtagctga tcttgtaagc cgtggtcgtt tcccctacag
     3361 aaagcctttt aagagtcttg gaaaagatga ccttgaaata ataaacagat caatggttat
     3421 ggccaatgtt gaagatctag caaataacct agttgaagaa ctttctgggg gtcaaaggca
     3481 aagagtatgg atagctctag ccctagccca agatacaagt atcctacttt tagatgagcc
     3541 aactacttac ttggatatct catatcaaat agaactatta gacctcttga ctgatctaaa
     3601 ccaaaaatat aagacaacca tttgcatgat tttgcacgat ataaatctaa cagcaagata
     3661 cgctgattac ctatttgcaa ttaaagaagg taaacttgtt gcagagggaa agcctgaaga
     3721 tatactaaat gataaactag ttaaagatat ctttaatctt gaagcaaaaa ttatacgtga
     3781 ccctatttcc aattcgcctc taatgattcc tattggcaag caccatgtta gctcttaata
     3841 attgaatatt ggaattcatt taaaagtcgc aaattttgaa ctgaccctca aaagttaggc
     3901 caaaaaacta actaaaggag gtcagttttt taatggcaaa tacagcacag aatttaagat
     3961 aaaattagta aaagaatatc atgaatgaaa aataggatat agagaataac agcaatatta
     4021 aaaagatcag gactgataat aaacaaaaag aaagtacaac gactagttca aaagctaaaa
     4081 cttcaagtaa aaagttattc aagaaaatct agaaaatact catcctacaa aggacaggta
     4141 ggaaaaatat cggacaacaa aataaaaaga aatatttatt accgaaaaaa aggggaaatc
     4201 gagtcaagta ccaacaggct catctattcc aaaaagatat ttactcgatg gaaaaactcc
     4261 tagaattagt gctacaaata ttgacaatgg tatcttaggt tactacgaag ata
//
LOCUS       DAVKLX010000085         4209 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKLX010000085 DAVKLX010000000
VERSION     DAVKLX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.085"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            57..823
                     /locus_tag="VW325_002039"
                     /pseudo
     CDS             57..823
                     /locus_tag="VW325_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1642..2001
                     /locus_tag="VW325_002040"
     CDS             1642..2001
                     /locus_tag="VW325_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735355.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            2011..2376
                     /locus_tag="VW325_002041"
     CDS             2011..2376
                     /locus_tag="VW325_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU9735356.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYEQLVFELRRIGNNINQIARAINQSHLISQNQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSY"
     gene            2363..>4209
                     /locus_tag="VW325_002042"
     CDS             2363..>4209
                     /locus_tag="VW325_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEU9735357.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSHAEMVEMYNVNFSNNDKLYESRNDRQEKHQQTIHAHYLIQSFSPEDNLTPEEINR
                     IGYEIMMELTGGRFKFIVATHTDKDHVHNHILINAIDRNSDKKLIWNYALERNLRMIS
                     DRISKVTGAKIIEKRYSYRDYKKYRESSHKFELKQRLYFLLQQSKSFDDFLEKAKQLH
                     VQIDFSQKHSRFLMTDRTMIKPIRGRQLSKRDLYDEDFFKIYFAKQEIESRLKFLLKS
                     VYSLEELHVKAKELNLTIELKQKNVMFTLEEDGKKISLSHKKISDKKLYDVQFFNRYF
                     EDREVRDIQALENLQEDFQTFREEQHKEKVSAEEIEEAFKKYKEKRDAIHEFEIELTD
                     NQIEKLVDDGVYIQVSFGIKQSGLIFIPNYQLDIFEEDNHKKYKVYIRETSSYFVYNK
                     ENMDNNCFIKGRTLIRQLSNDSQKLPYRRPTLKSLKEKISEINLMIELSNTNKQYQEI
                     KDELVLEIAEIDMQLEETQEKIATLNKMAEVLINLKSEDHETRKLAKYDFDQMNMTES
                     IMLDRLNTDILKLQQELGNEINKYEEIARRLDLFVKIINTNKFTVLKFH"
BASE COUNT         1532 a          630 c          827 g         1220 t
ORIGIN      
        1 ctgatcataa tactaccaaa ggtattaaaa agttacaaat ggcagtctca atcataatga
       61 aaaattatcc gatttatgat atacatcctc atattttaca tggagtagaa attagtgcag
      121 cagataaatt gcatattgta tgtatatatg attatgaaca agaatcatgg gttaatcaat
      181 ggttaagtga aaatattata agtgagaaag atggaagtta tcaacattca ctgactataa
      241 tgaaggattt caataatcaa aaaatagtta actatattgc tcatttcaat agttatgaca
      301 ttttgaaaaa aggttctcac ttatcaggtg catataaacg aaaaattttt tctaaagaaa
      361 atacacgatt ttggagttta atattaactc gaaagaatct tcgcaacaac ttgatattct
      421 ctataaagaa gttggtgtat taagtttggg acaaaaagtt gtagccatgc ttgatttttt
      481 attagcatat agtgattatt ctaaagactt cagaccattg attattgatc agcctgaaga
      541 caatctagac aatcgttata tttgcaggca tttagttcag cagtttagag atgtgaaagc
      601 tcaacgtcaa attattttag caacacataa tgctacaatt gtaacaaatt ctatgacaga
      661 tcaagttgtt attatggagt cagatggagt taacggatgg attgaatcac agggatatgt
      721 tagtgaaaaa tatataaaaa atcatatcat caatcaatta gagggaggaa aagattcctt
      781 caagcataaa atgtctatat atgagacggc tttatcagag tagagtcaga aaaagtaggt
      841 tagaaattta gcctactttt ttctttgtcc gacaggcata gtgtgcatct gaggtccaag
      901 tcctctgtgg atatttgctg cagatgaaac caatagcgac tcctaagcct gaatatcgtg
      961 aggtaggggg gataggaagg aattagcgaa atcaaggttc tacaaacaga atcgtgactt
     1021 gaagccatat atagcggatg aggaactcta aaatccaaat aggtgtcgta acctatatac
     1081 gtaaattacg agagtaaact aggaaagatg tacggcttat tccgtgagcg tttaggacgt
     1141 agtacaacga atcatgggag tcagctgaac acatagtatt gaagaaattt ctgtaatgga
     1201 aatggagcga agaagtgaac aattaaatga atacctctct aattaaattt gtcaattcta
     1261 attcctggta tgaaaagaca gtgacctgaa aatgtaaacg atgggagctg attataaata
     1321 taggacggta catgcagtgg tgttagagat tagtccctac ttgatttgtg ataacttccc
     1381 caaatttctt ctgctatact tttctcaact tttaaaaatc caactaagaa ttttacctgg
     1441 gggtttgggg gcgaagccac caagttatct tatcgttggc tgtcaaaact ggaaggtttt
     1501 ggttagctgg cgatatgatt ttttgggtat tgtaaccaca atgcttgagc tcgcaaagac
     1561 cgaacaagga ctttaagaaa tttcccgtat gacacacggg gtcggggttt acccgagaaa
     1621 ggatgaaagt gaggaaaaga gatgtctgaa cagtaccgtg acattcgaaa agaggtaaat
     1681 ctaaccgcag atgagctaaa acagattgaa aaaatgatgg aggttgataa ttatcgtcac
     1741 ttttcaccat tcgtcaggga taaaattttg atgactgatg acaaacagtt agctgccaaa
     1801 gaatggtttt ccctttggca gtctcaaaag tttgaacaga tcagtcgaga tgtgcatttg
     1861 gttttgatta tcgcaagaga gaatcaccaa gtgactcaag aacacgtgtc aatcttattg
     1921 acctgcgttc aagaattgat tgcggaagtc aatcaagtac agtcactcag tcgtgggttt
     1981 cgtgaaaaat acatgagata ggaggctact atggtttatc gttatcgtac caatctcaaa
     2041 aaagtatttc taacagattc agaattacac caattgaatg aacggattgc taagagcaat
     2101 tgtcaaaatt tctcagtcta tgccagaaaa gttttgctta atcccaatat gtcatttgtc
     2161 acgattaaca ctgatactta tgaacagtta gtgtttgaat tgaggaggat tggaaataac
     2221 atcaatcaaa ttgcgcgtgc gattaatcaa agccatctga tttctcagaa ccaattacaa
     2281 gaattgagta aaggagtcgg agagttaatt aaggaagtgg acaaagaatt tcaagtggag
     2341 gtgaaaagac tgaaggagtt tcatggtagt tactaaacac tttgcgacgc acggaaaaaa
     2401 atatcgtagg cgtttaatta agtatattct caaccctgat aaaacggaca atttgaaatt
     2461 ggtatctgat tttggcatga gcaattactt ggacttccct agtcatgcag aaatggtaga
     2521 gatgtacaat gtcaacttca gcaataatga caagttgtac gaatccagaa acgaccgaca
     2581 agaaaaacac caacaaacta ttcatgctca ttacctcatt caatcatttt ctcctgaaga
     2641 taatctgaca cctgaagaaa ttaaccgcat tggttatgag atcatgatgg aattgacagg
     2701 aggtcgtttt aagtttatcg tagcaactca cacagacaaa gatcatgttc ataatcacat
     2761 cctaatcaac gctattgacc gcaattcaga taaaaagttg atatggaact atgccttgga
     2821 acgaaacctc cgtatgattt cagaccgtat ttctaaagtg actggtgcaa aaattattga
     2881 gaaacgttat tcctatcgag attataaaaa atatagggaa tctagtcata agtttgaatt
     2941 gaaacaacgt ctttattttt tgctacagca atctaaatcg tttgatgatt ttttagagaa
     3001 agcaaagcag ttgcatgttc agattgattt tagtcaaaag catagccgtt ttttgatgac
     3061 agatagaacg atgataaaac caattcgagg acgccaactc agcaaacgag atttatatga
     3121 tgaagacttt tttaaaatat attttgccaa acaagagatt gaaagtcgtt taaaattttt
     3181 actaaagtct gtttattcat tagaagaatt gcatgtaaag gctaaagagt tgaatctaac
     3241 catcgagtta aaacagaaaa atgtgatgtt taccctagaa gaagatggca aaaagataag
     3301 cctgagtcat aaaaaaatta gcgataagaa attgtacgat gttcaatttt ttaatcgtta
     3361 ttttgaagat agagaagtta gagatattca agcattagaa aatcttcagg aagattttca
     3421 gacttttcga gaggagcagc ataaggagaa agtctccgct gaagaaatcg aggaagcctt
     3481 caagaaatac aaggaaaaac gagatgctat ccatgagttt gagattgaac ttacggataa
     3541 tcagatagag aagttggttg atgatggtgt ctatatacag gtttcgtttg gtatcaaaca
     3601 gagtggactc atctttattc ctaactacca gctagatatt tttgaggaag acaatcataa
     3661 aaaatataaa gtctacattc gagaaacgtc ttcttacttt gtctataata aagaaaacat
     3721 ggataataat tgtttcatca agggtaggac cttaatcaga caattgagca acgatagcca
     3781 aaaactcccc tatagacgac caactttaaa aagtcttaaa gagaagattt ctgaaattaa
     3841 tcttatgatt gaactatcta acacgaacaa acaataccaa gaaatcaaag atgagcttgt
     3901 attagaaatt gcagagattg atatgcagct agaagaaacc caagaaaaaa tcgccacctt
     3961 aaacaagatg gcggaagtcc ttatcaattt gaagagtgaa gatcatgaga ctagaaaatt
     4021 agctaagtat gattttgacc aaatgaacat gacggaatca ataatgttag atcgactcaa
     4081 tacggatatt ttaaaattac aacaggaact tggtaatgaa attaataaat atgaggagat
     4141 agctagaaga ttggatttgt ttgtgaaaat tattaatacc aataaattta cagttctaaa
     4201 atttcacga
//
LOCUS       DAVKLX010000086         4053 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKLX010000086 DAVKLX010000000
VERSION     DAVKLX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.086"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..629)
                     /locus_tag="VW325_002043"
     CDS             complement(<1..629)
                     /locus_tag="VW325_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9735358.1"
                     /translation="MIKLEFLKQKKSILWFVLIFPIILNVLLYIDLTFRYRGYLLVHQ
                     NELALSNWQLIFKEQTIFYFSELFYLVLSLIIYEVFAVEFKNDAWLTVISLPFRNKYT
                     INSKLLITVVYTFTFWLSDYISLYVIGKAIDNSLEIGLIFFLKTFTIQLISSLMIMLL
                     YFLTLVLIRKISGIIPIGIIMMILTISIYYNDYNFKIYLPFTYLSHAFRV"
     gene            complement(622..1350)
                     /locus_tag="VW325_002044"
     CDS             complement(622..1350)
                     /locus_tag="VW325_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9735359.1"
                     /translation="MAIVEIINLTKSFKDIEVIHNTSFYLNKGKVYGFVGPNGAGKTT
                     IIKMILGILKPDSGKITIFNQTVEQNSENILSRIGLVLGPSFYGHLDAYKNLKLIANM
                     KGLSLDTERLNEYLSMVGLKDVKKKKVKNFSMGMKQRLSIAASLLGSPEILIWDEPIN
                     GLDPQGVIEIRSLIRFLQEKKGITFLISSHILSELDKVISDIIIINYGKVEFFGSCHY
                     LLQKYNCRNLEEAYLACLAGGEYD"
     gene            complement(1384..1554)
                     /locus_tag="VW325_002045"
     CDS             complement(1384..1554)
                     /locus_tag="VW325_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735360.1"
                     /translation="MKKVNEYVISTAASLGVMIGIVFAIFLDFPVEYAISLGLLNGIV
                     LGSLIFYKNNKN"
     gene            complement(1567..3690)
                     /locus_tag="VW325_002046"
     CDS             complement(1567..3690)
                     /locus_tag="VW325_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEU9735361.1"
                     /translation="MTRKVPNIEQMSQIECGLCCCLSILHFYKSKETLLDLRRDIEKG
                     RDGYSIGDLKQLLNKRNFDTGSYQVKDVNKISELPLPLIAFWDNQHYVVIYKVKKNKV
                     YIMDPSKGYINYEFKEFSKHFSNIVLLSFPNENYQSLKSQFPSPWIRVFSSFSKVKGR
                     LILTLLFSIISYLIILSVPVMTSKFINSALGNTFSFQTSFLILFSLLCLYLISILARS
                     MGILFSNIFFSRDIESFTFKHLLKLPYSFFELRAKGDILYRISSLSGFRELFTNQVVG
                     GVVDIGTILSVVIYMFLSSKTLSIIALILSLINFLFLFSTRKIMYDTVNRELQEQSLI
                     YSVETEALNTISSIKISGLEDEIYENWSKYLKNVLTKYKKRSIVHILYNSATNVFQLF
                     APIIILIFGLDNVLNGKILLGEVVAFQTMASILFSSEISIFNAYTQYILAAGYLNRVN
                     DIWLENEENVENGLKKCSLEGRIDIKDLSFSYSKDSAPVIENLNLTIEPGQRIALVGQ
                     SGSGKSTLSKILSGLYKIDTGKILFDGVNINQIDKKILSQNLGVVPQDSFLLNRSILD
                     NITLKNEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRIALARAL
                     INNPSIVILDEATSALDTINEERITKYIQSQGCTQIIIAHRLSTIKDADIIFVMKGGK
                     IVESGNHKYLMDLGGEYYSLYTKRK"
     gene            complement(3683..>4053)
                     /locus_tag="VW325_002047"
     CDS             complement(3683..>4053)
                     /locus_tag="VW325_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735362.1"
                     /translation="LARKEIFDKTNIGNNDLSIIKNVLTNVEKIRELNLCHGSVGTIS
                     CLDAILKDEENLLIKESIDFYFDNVVSQVIKPELSTDLNTMNTFSFMLGVSGVVYEIS
                     RKQDDRLLNVLLLELRGHDD"
BASE COUNT         1448 a          694 c          456 g         1455 t
ORIGIN      
        1 actctaaaag catgagaaag ataagtaaat ggtaagtaaa tcttaaaatt ataatcgttg
       61 tagtatatgc taattgttaa aatcatcatt ataataccaa ttggtattat tccagaaatt
      121 ttccttataa gtactaaagt tagaaaatat aataacatta tcatcaacga ggaaattaat
      181 tgaatggtaa atgtttttaa aaagaaaatc aaaccaatct ctaaactgtt atcaatagcc
      241 ttcccaataa cgtataagct aatataatca gacaaccaaa aagtaaatgt gtaaaccact
      301 gtaatgagta atttagaatt tattgtgtac ttgtttctaa atggcaatga aattacggtt
      361 aaccatgcat catttttaaa ttctactgca aacacttcgt aaattattaa agatagaact
      421 aaataaaata gttcagaaaa ataaaatatt gtttgttctt taaaaattaa ctgccaatta
      481 gataaagcaa gttcattttg atgcaccaat aaataacctc gatacctaaa agttaagtct
      541 atatataaca aaacattcag aattattgga aaaataagaa caaaccataa gatgcttttt
      601 ttctgcttta gaaactctaa tttaatcata ttcacctcca gctaaacaag ctaagtaggc
      661 ttcttccaga tttcgacaat tatacttttg taaaaggtag tggcaagaac caaaaaattc
      721 aactttccca taatttataa ttataatatc agatatcact ttatccagtt cactaagaat
      781 atgacttgat attaaaaatg taataccttt tttctcctgt agaaaacgga ttaaacttct
      841 aatttcaatt acaccctgtg gatcaagtcc attgattggt tcatcccaaa ttaatatttc
      901 tggactacct aacaatgatg cagctatact tagacgttgt ttcatgccca ttgaaaaatt
      961 tttcactttt ttctttttaa catcttttaa tcctaccatt gatagatatt cattaagtct
     1021 ttcagtatct aaactaagcc ctttcatatt tgctattaac ttcaaatttt tataagcatc
     1081 aagatgacca taaaatgaag gtcccaaaac taatcctatc ctagataata tattttcgga
     1141 attctgttct acggtttgat taaaaatagt tattttccca gaatctggtt ttaaaatccc
     1201 tagaatcatt ttgataattg tagtttttcc agccccattt ggtccaacaa aaccatatac
     1261 ttttccctta tttaaataaa atgaagtgtt atgaataact tcaatatctt taaagctttt
     1321 tgttagattt ataatttcaa ctatagccat ctctactcct tagtttacag caaaaaatta
     1381 tgcttaattc ttattgtttt tgtaaaaaat cagcgaaccc aatactattc cattcaacaa
     1441 acctaaagaa atagcatatt caactggaaa atctaaaaaa attgcaaata ctattccaat
     1501 catcacccct agtgaggctg ctgttgaaat tacatattca tttactttct tcattttctt
     1561 tacacctcat ttcctttttg tatataagct gtagtactct ccaccaagat ccattaagta
     1621 cttatgattt cctgactcaa caatcttacc acctttcatt acaaaaataa tatccgcatc
     1681 cttaatcgtt gacaatctat gagctataat tatttgagta caaccctgac tttgtatata
     1741 ctttgttatt ctttcctcat taatagtgtc taatgcacta gttgcttcat ctaaaattac
     1801 aatactagga ttatttatta atgcacgtgc cagtgctatc cgttgccttt gtccacctga
     1861 aatatttgac cccatctctg agatgatagt attaaatttc atcggcatag ccatgatttc
     1921 atcatagatt tgaactgctt tacaaacttc ctctatcttt tgtgaagtaa cttcgttctt
     1981 taaagttata ttatcaagaa tacttcggtt caataaaaat gaatcctgtg gaactactcc
     2041 taaattttga cttaatattt ttttatctat ttgattaata tttacaccat caaataaaat
     2101 ttttcctgta tcaatcttat ataatcctga tagtattttt gataacgtac tttttcctga
     2161 ccccgattga ccgactagtg caattctctg tcctggctca atagtcaaat ttaaattttc
     2221 tattacagga gctgagtctt tggaataaga gaaagatagg tccttaatat ctattctacc
     2281 ttctaaagaa cactttttaa gaccattttc aacattctct tcattttcaa gccagatatc
     2341 atttactcta tttaagtatc ctgctgccaa aatatactgc gtataagcat taaatattga
     2401 gatttctgaa ctaaataaaa ttgatgccat ggtttgaaat gcgacaacct cacccaataa
     2461 aatttttcca tttaaaacat tatcaagtcc aaaaatcaat ataattatag gagcgaacaa
     2521 ttgaaataca ttagtagctg aattgtataa aatatgcaca atacttcgtt ttttatactt
     2581 tgtcaatacg ttttttaaat acttagacca gttttcatat atttcatctt ctaatcccga
     2641 tatttttatt gaagaaatag tatttaatgc ttctgtttct actgagtaaa tcaatgattg
     2701 ttcttgtaat tctctattaa ccgtatcata cattattttt ctagtactaa acagaaacaa
     2761 aaaattgatt aaactcaata taagagctat aattgataat gtttttgaac taagaaacat
     2821 atatattaca acacttagaa tagtcccgat atctactact cccccaacaa cttggttagt
     2881 aaatagctcc ctaaaaccac taagactaga aattctgtat aaaatatctc cttttgccct
     2941 gagttcaaaa aaactatatg gtagtttaag caaatgctta aatgtaaaac tttcaatatc
     3001 tctactaaaa aatatattag aaaatagaat tcccatactc ctagccaaga tagaaataag
     3061 atacaaacaa agtagagaaa ataaaattaa aaacgaggtt tgaaaagaaa atgtattccc
     3121 taatgcagaa ttaataaact ttgaagtcat tactggtacg gaaagtatta ttaagtaact
     3181 tattattgaa aatagtaacg tcaaaatcaa tcgacctttt acctttgaaa aactagagaa
     3241 tactcttatc catggagaag gaaactgcga cttcaaagat tgataatttt cgttaggaaa
     3301 tgatagcagt actatattac taaaatgttt tgaaaactcc ttaaactcat aatttatata
     3361 tcctttagaa ggatccatta tatagacctt attctttttt actttgtaaa taactacata
     3421 atgttgatta tcccagaatg ctattaatgg taacggaagt tcactaattt tatttacatc
     3481 tttaacttga tatgagccag tatcaaaatt tcgtttattc aataactgtt tcaagtcacc
     3541 tatactataa ccatctctcc ctttttctat atcacgtctt aaatctagta atgtctcttt
     3601 gcttttatag aaatgtagta tggacaagca gcaacataaa ccacattcta tttgagacat
     3661 ttgctcaata ttaggtactt ttctagtcat catgtcctct taactccagt aataaaacat
     3721 taagtaatct atcatcttgt tttcgagata tttcatagac aactccagat actcctagca
     3781 taaaagaaaa tgtattcata gtattcaaat ccgtacttaa ttctggtttg atgacctgag
     3841 aaacgacatt atcaaaataa aaatctatag attcttttat taataggttt tcttcatctt
     3901 tcaatattgc atcaagacaa ctgatagtac ctacagaacc gtgacataaa ttcaattctc
     3961 ttattttttc tacattagtt aatacatttt taattattga taaatcatta ttcccaatat
     4021 tagttttatc gaaaatttct tttctagcta ata
//
LOCUS       DAVKLX010000087         3996 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKLX010000087 DAVKLX010000000
VERSION     DAVKLX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.087"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            332..559
                     /locus_tag="VW325_002048"
     CDS             332..559
                     /locus_tag="VW325_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735363.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            847..3792
                     /locus_tag="VW325_002049"
     CDS             847..3792
                     /locus_tag="VW325_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU9735364.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSEEGENITLPAEHVESVSETELSGNKQEQERKDK
                     QEEKIPRDYYARDLENVETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFK
                     PDAKAPAFYNLFSVSSATKKDEYFTMAVYNNTATLEGRGSDGQQFYGNYNDAPLKVKP
                     GQWNSVTFTVEKPTAELPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRA
                     NNTVWGSNLQIRNLTVYNRALTPEEVQKRSQLFKRSDLEKKLPEGAVLTEKTDIFESG
                     RNGKPNKDGIKSYRIPALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWG
                     DKVVISNLRDNPEAKDPAAPSPLNIDMVLVQDPTTKRIFSIYDMFPEGRAVFGMPKTP
                     EKAYEKIGDKTYQILYKQGESGHYTVRENGEVYNAQNQKTDYRVVVNPTEPGYRDKGN
                     LYKGQELIGNIYFAHSTKNPFRVANTSYLWMSYSDDDGKTWSAPRDITPGLRKDWMKF
                     LGTGPGTGIVLRNGPHKGRILIPVYTTNNVSHLDGSQSSRVIYSDDHGKTWHAGEAVN
                     DNRQVDGQKIHSSTMNNRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTW
                     EKDIKRYPQVKDVYVQMSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTW
                     LKHNPIQKGEFAYNSLQELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKNLISPT
                     EAKVKRTREMGKGEMGKGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFA
                     VDKEDIGQEIIGIAKGSIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGIE
                     PAVHEIAEYKGSDSLVTLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGL
                     FTLGKKREQ"
BASE COUNT         1373 a          635 c          895 g         1093 t
ORIGIN      
        1 tcatgcggct agcttcttag tttgctcttt gattttcatt gagtataaaa acagatgagt
       61 ttctgttttc tttttatgga ctataaatgt tcagctgaaa ctactttcaa ggacattatt
      121 atataaaaga attttttgaa actaaaatct actatattac actatattga aagcgtttta
      181 aaaatgaggt ataataaatt tattaacact tataaaaagt gatagaatct atctttatgt
      241 atatttaaag atagattgct gtaaaaatag tagtagctat gcgaaataac agatagagag
      301 aagggattga agcttagaaa aggggaataa tatgatattt aaggcattca agacaaaaaa
      361 gcagagaaaa agacaagttg aactactttt gacagttttt ttcgacagtt ttctgattga
      421 tttatttctt cacttatttg ggattgtccc ctttaagctg gataagattc tgattgtgag
      481 cttgattata tttcccatta tttctacaag tatttatgct tatgaaaagc tatttgaaaa
      541 agtgttcgat aaggattgag caggaagtat ggtgtaaata gcataagctg atgtccatca
      601 tttgcttata aagagatatt ttagtttaat tgcagtggtg tcctggtaga taaactagat
      661 tggcaggagt ctgattggag aaaggagagg ggaaaattgg caccaatttg agatagtttg
      721 tttagttcat ttttgtcatt taaatgaact gtagtaaaag aaagttaata aaagacaaac
      781 taagtgcatt ttctggagta aatgtcttat ttcagaaatc gggatataga tatagagagg
      841 atcagtatga atcggagtgt tcaagaacgt aagtgtcgtt atagcattag gaaactatcg
      901 gtaggagcgg tttctatgat tgtaggagca gtggtatttg gaacgtctcc tgttttagct
      961 caagaagggg caagtgagca acctctggca aatgaaactc aactttcggg ggggagctca
     1021 accctaactg atacagaaaa gagccagcct tctgaggaag gagaaaatat aactcttcct
     1081 gcagagcatg tagagtctgt ttcagagact gaactttctg gcaataagca agaacaagaa
     1141 aggaaagata agcaagaaga aaaaattcca agagattact atgcacgaga tttggaaaat
     1201 gtcgaaacag tgatagaaaa agaagatgtt gaaaccaatg cttcaaatgg tcagagagtt
     1261 gatttatcaa gtgaactaga taaactaaag aaacttgaaa acgcaacagt tcacatggag
     1321 tttaagccag atgccaaggc cccagcattc tataatctct tttctgtgtc aagtgctact
     1381 aaaaaagatg agtacttcac tatggcagtt tacaataata ctgctactct agaggggcgt
     1441 ggttcggatg ggcaacagtt ttacggtaat tacaacgatg cacccttaaa agttaaacca
     1501 ggtcagtgga attctgtgac tttcacagtt gaaaaaccga cagcagaact acctaaaggc
     1561 cgagtgcgcc tctacgtaaa cggggtatta tctcgaacaa gtctgaaatc tggcaatttc
     1621 attaaagata tgccagatgt aacgcatgtg caaatcggag caaccaagcg tgccaacaat
     1681 acggtttggg ggtcaaatct acagattcgg aatctcactg tgtataatcg tgctttaaca
     1741 ccagaagagg tacaaaaacg tagtcaactt tttaaacgct cagatttaga aaaaaaacta
     1801 cctgaaggag cggttttaac agagaaaacg gacatattcg aaagcgggcg taacggtaaa
     1861 ccaaataaag atggaatcaa gagttatcgt attccagcac ttctcaagac agataaagga
     1921 actttgatcg caggtgcaga tgaacgccgt ctccattcga gtgactgggg tgatatcggt
     1981 atggtcatca gacgtagtga agataatgga aagacatggg gagataaggt ggttatctcc
     2041 aatcttcgag ataatcctga agctaaagat cctgctgcgc catcgcctct aaatattgat
     2101 atggttttgg ttcaagaccc gacaacaaag agaatcttct caatttatga tatgttccca
     2161 gaaggtcgag cagtttttgg aatgccaaaa acacctgaaa aagcttatga aaagataggg
     2221 gataaaactt atcaaatctt gtataaacaa ggagagtctg gtcattatac tgttcgtgag
     2281 aatggagaag tgtataatgc acaaaatcaa aagacggatt atcgtgttgt agtgaatcca
     2341 acagaacctg gctatagaga taaaggaaat ctttacaaag gtcaggaatt gattggaaat
     2401 atctattttg cacacagtac aaaaaatcca tttagagtag ccaatacgag ctatctatgg
     2461 atgtcatata gtgacgatga tggtaaaact tggtctgcac cgagagatat tactccaggt
     2521 cttcgcaagg attggatgaa gttcctagga acaggtcctg gaacaggaat tgtacttcgg
     2581 aatgggcctc acaagggacg gattttgata ccggtttata cgactaataa tgtatctcac
     2641 ttagatggct cgcaatcttc tcgtgtcatc tattcagatg atcatggaaa aacttggcat
     2701 gctggagaag cggtcaacga taaccgtcag gtagacggtc aaaagatcca ctcttctacg
     2761 atgaacaata gacgtgcgca aaatacagaa tcaacggtgg tacaactaaa caatggagat
     2821 gttaaactct ttatgcgtgg tttgactgga gatcttcagg ttgctacaag taaagacgga
     2881 ggagtgactt gggagaagga tatcaaacgt tatccacagg ttaaagatgt ctatgttcaa
     2941 atgtctgcta tccatacgat gcacgaagga aaagaataca ttatcctcag caatgcaggt
     3001 ggaccgaaac gtgaaaatgg gatggtccac ttggcacgtg tcgaagaaaa tggtgagttg
     3061 acttggctca aacacaatcc aattcaaaaa ggagagtttg cctataattc gctccaagaa
     3121 ttaggaaatg gggagtatgg tatcttgtat gaacatactg aaaaaggaca aaatgcctat
     3181 accctatcat ttagaaaatt taattgggac tttttgagca aaaatctgat ttctcctacc
     3241 gaagcgaaag tgaagcgaac tagagagatg ggcaaaggag agatgggcaa aggagttatt
     3301 ggcttggagt tcgactcaga agtattggtc aacaaggctc caacccttca attggcaaat
     3361 ggtaaaacag cgactttcct aacccagtat gatagcaaga ccttgttgtt tgcagtagat
     3421 aaggaagata tcggacagga aattattggt atagctaaag gaagcatcga aagtatgcat
     3481 aatcttcctg taaatctagc aggtgccaga gttcctggcg gagtaaatgg tagcaaagca
     3541 gcggtgcatg aagttccaga atttacaggg ggagttaatg gtatagagcc agctgttcat
     3601 gaaatcgcag agtataaggg atctgattcg cttgtaactc ttactacaaa agaagattat
     3661 acttacaaag ctcctcttgc tcagcaggca cttcctgaaa caggaaacaa ggagagtgac
     3721 ctcctagctt cactaggact aacagctttc ttccttggtc tgtttacgct agggaaaaag
     3781 agagaacaat aagagaagaa ttctaaacat ttgattttgt aaaaatagaa ggagatagca
     3841 ggtttttaag cctgctatct ttttttgatg acattcaggc tgatacgaaa tcataagagg
     3901 tctgaaacta ctttcagagt agtctgttct ataaaatata gtagattgaa ataagatgtg
     3961 aacaactcta tcaggaaagt caaattaatt tataga
//
LOCUS       DAVKLX010000088         3948 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKLX010000088 DAVKLX010000000
VERSION     DAVKLX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.088"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(191..802)
                     /gene="rpsD"
                     /locus_tag="VW325_002050"
     CDS             complement(191..802)
                     /gene="rpsD"
                     /locus_tag="VW325_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9735365.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(997..2049)
                     /locus_tag="VW325_002051"
     CDS             complement(997..2049)
                     /locus_tag="VW325_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9735366.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2046..2744)
                     /locus_tag="VW325_002052"
     CDS             complement(2046..2744)
                     /locus_tag="VW325_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9735367.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2911..>3948)
                     /gene="pavB"
                     /locus_tag="VW325_002053"
     CDS             complement(2911..>3948)
                     /gene="pavB"
                     /locus_tag="VW325_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9735368.1"
                     /translation="WSDNGDAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALI
                     DQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTININGLISKETVQKAVADNVK
                     DSIDVPAAYLEKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAK
                     NPALSPLGENVKTKGQYFYQLALDGNVAGKEKQALIDQFRANGTQTYSATVNVYGNKD
                     GKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPAD
                     TMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLG
                     GLGLKNKKEEN"
BASE COUNT         1058 a          880 c          780 g         1230 t
ORIGIN      
        1 gccataaaag gattatacca cattgtgtac tatttttggt tcattttact atatcacttt
       61 ggactatatc ggtctagtta tgtctcaacg attatagctg gatactaaga gaaatcaaaa
      121 aagcactaag ggagaacgtc ccaaagtgct taatatcaag gcctcaaagt ctatcttatt
      181 caataaaata ttacaacatt ttgttgtaga attcaacgac aagtgcttcg ttgatttctg
      241 ggttgatttc gtcgcgttct ggcaagcgag tcaatgaacc ttccaacttt tcagcgtcga
      301 atgatacgaa tgctggacgt ccaagagtag cttctactgc ttcaaggatt gctggaactt
      361 tcaatgattt ttcacgaact gagatcactt gacctggagt tacacggtat gatgggatat
      421 caacgcgttt cccgtcaaca aggatgtgac cgtggtttac gaattgacga gcttgacgac
      481 gagtagtcgc gagaccaaga cggtaaacaa cgttatccaa acgacgttcc aaaagaagca
      541 taaagttgaa acctaggatt ccgcctttga tttttgtagc ttgtacgaac aagttacgga
      601 attgtttttc acctacaccg taagtgaaac gaagtttttg tttttcagcc aattgcaaac
      661 cgtattctga caatttagaa cggttgtttg gtccgtgttg tcctggtacg tagttacgac
      721 gtgccaattc tttacctgta cctgtaagtg aaaggccaag gcgacgagct tgtttccaag
      781 atggtcctgt ataacgtgac atatgaatgt cctcctgata taaataatat ttcggtggaa
      841 atagtcactt agaaagccct gattcgtgca gatgcccttc gcctaaacag ccaaggttac
      901 ttgtcataag acacctgttg acgagcttca tgctttcctg ctgatatttc acacaaaatc
      961 tattctacca tgaatagctg gatttgtaaa ggggttttag gctttatttt cactaccaga
     1021 gaggttgaga acgagggtaa aggtacttcc tagaccgtac tggctgctga ctgtgatttc
     1081 cccacccaat tgatgggcca attcacgcgc aatcgcaagt cctaatccat gaccacctgt
     1141 cttcatgtta cgcgaagttt cgacacgata aagacgtttg aaaatatttt ccaaatcctc
     1201 tggggcaata ccctgccctt catcggtcac actgattgaa agctggtcct tctccagctt
     1261 agccaccact tccagcttgg ttcctggagc agaatattta aaagcgttat cgaccagatt
     1321 caccaagata cgagaaagct tagcataatc tccctcaatc cgggcagact ctgggattac
     1381 ctgcaagtgg acatctcttc tctcctgctc aatcaaaaac tgaaattcac tcatgcactc
     1441 aattaagagc ttgtccagaa aaatactgtc tttactggta gtttccacct gattcctagc
     1501 tgtgtttagg gtcaaaaaat tcaactcctc aaccagttta ttgagcctct ccgtctggcg
     1561 tccaatggtt gctagataat gagcttgctc cgactccttg ataatcccat ccaaaatccc
     1621 ttctaccgtc gcttggatcg aagtgatagg agtcttaata tcatgcgaca actgggcaat
     1681 catcaagccc ttttctcgtt cgctttcttc caaggaatca aagcttacct gcaaatcatg
     1741 ggacatctca ttaaaagttt gccctaattg ctgaaattct acaggacctt gaacctccaa
     1801 atttgaagga aaatccttgg ccgctacccg cttggcatgc tccttgagtt tgcccaacga
     1861 cgtaaagact ggcaatagga gaaagagact aatcccagca ccgacaaggc tggcaacgat
     1921 ggtcatccca agcaaaaagt aaatctcgcc tttcgcaatc agcatttttt gaacagccca
     1981 aaaaacaacc aaaatggtta agagggttga aataatatat ccaaccaaaa tataactttt
     2041 tagtttcatg tttgtcctct cggtttctct atcttatatc ccaaccccca aactgtctta
     2101 atagtgggcg tttggtcact actatatttt gccagctcct gtcgaagagc atggatatgc
     2161 acattcaagg tattggtgtc atccacgtag tcttctttcc agatcttttc atagaggtct
     2221 gtcttggaga aaactcgctc tggattgcta gctaaaatcc acagcaattc aaatgattta
     2281 acagttaaat ccagcatttc ttctcctatt tgaacttcat gactactatg attcattttt
     2341 agattgccaa gggaaatcag ctctgtttcg cccccacgat gaaggcggcg caaaatattg
     2401 tggacacgca aaaccagctc acgtgggcta aaaggcttag caataaaatc atctgctccc
     2461 aagctcaggc cgtaaatctt gtcctgttca ctggtcttag cagtaataaa taggaaaggc
     2521 tgctctggtg ataagtattg aacctcactg attaaatcat aaccatccat ccgaggcatc
     2581 atgacatctg tgataatcaa atcaatcggt tttttcttga acagctctag cgcttccagt
     2641 ccatcatggg ctaccaagac ctgataacct gcctgaatta agtatctctg atgaatatct
     2701 gtgatttcta cctcgtcgtc aacgagtaaa attgtctttc ccatctgtct ctcctttgat
     2761 aaaaacagtg ctatactgct ttaagtataa cactattttt attgttttac gatgatttta
     2821 atctgctatt gaaagaaagt cctgattgct gattgagttg ttaaactaat cttagtcagc
     2881 ccactatcca tcatttattt ccttagctga ttagttttct tcttttttgt ttttcaaacc
     2941 tagaccaccg agtaaacctg caagcgcaag cccaaggaaa ccaatacttg ccattgatgt
     3001 ttgagtctca ccagtatttg gtagcatagc tttatcctct gacatcatcg tatcagacat
     3061 cttgttagca gaagcagcca tgttttcacc tgccatcgtg ttggtagaac ttgtcatggt
     3121 gtcagcaggc atgctatctg tagtacctgt agcatgattg tgattcatcg gagtcacgcc
     3181 agaaccagag ttagaaggtg ataatgaacc atttgctgtg tctgatgttt ctttaacatt
     3241 tatcttaata gtgacttttt tagttgctac gatgttgtcc aaatctggtt taccgtcttt
     3301 gttaccatag acattgactg tagcgctgta agtttgagta ccgtttgctc ggaactggtc
     3361 aatgagcgct tgtttttctt tgccagctac atttccgtcc aaggctaatt gatagaagta
     3421 ttgacctttg gtcttcacgt tttcacctag tggagatagg gctgggtttt tagcgtcacc
     3481 gttatctgac catggtgcct tgtcagatgc cttgagcaag agacgagtca acataccatc
     3541 acctgcgaag agttcgtatg gaatcacatg gttgacacct gctgtgaatg gaccttcacc
     3601 cttggctttt tctaggtagg ctgctggaac atcgatactg tccttaacgt tgtctgcaac
     3661 ggctttttga actgtttctt tagaaattaa accgtttatg ttaatagtga cttttttagt
     3721 tgctacgatg ttgtccaaat ctggtttacc gtctttgtta ccatagacat tgactgtagc
     3781 gctgtaagtt tgagtaccat ttgctcggaa ctggtcaatg agcgcttgtt tttctttgcc
     3841 agctacattt ccgtccaagg ctacttgata gaagtattga cctttggtct tcacgttttc
     3901 acctagtgga gatagggctg ggtttttagc gtcaccgtta tctgacca
//
LOCUS       DAVKLX010000089         3689 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKLX010000089 DAVKLX010000000
VERSION     DAVKLX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.089"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            805..2964
                     /gene="pfbA"
                     /locus_tag="VW325_002054"
     CDS             805..2964
                     /gene="pfbA"
                     /locus_tag="VW325_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9735369.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTIKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNGNGKETVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNIQINDKIDNKQDNKTEEVNHKIVGDGIETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGISLLLTLKGLKYYGKDK"
     gene            2948..3689
                     /locus_tag="VW325_002055"
                     /pseudo
     CDS             2948..3689
                     /locus_tag="VW325_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239016.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
BASE COUNT         1405 a          459 c          671 g         1154 t
ORIGIN      
        1 aatattgttc aaagttttgt gcctgtcttt catgttctag tcattctttt gcatgataga
       61 atttatagca tattgatatt ataataatac aaatattcta tacgtttagt gatgcttgct
      121 atacatcatt agatctcccg cgagacaatc tataaaacac ttgtctacga ttacctatat
      181 gccctattcc agtattttag aagcactgca tctattttta tcgaggttaa atctagcttt
      241 tatagaaggt ctatttaaga aatatattgt agtgttttag tttcaatccg ccatatgagc
      301 gatattcagg taaatatccc tagcgaatgc ttgtatgaca agatatttgt tctttcattt
      361 ataatttaca acatatcaac aaatttaaat atagtaaatg ggatatttta tattcaagct
      421 aagaaagata gcatcacttt tgaatggaag gctaaagagc aaactaggaa gttggccata
      481 gatagctcaa aaccctgctt tgaggttgta gatataataa aatgaaataa gaataggaca
      541 aatcgatcgg gacagtcaaa tcgatttcta acaatgtttt agaagtagag gtgtactatt
      601 atagtttcag tctactatag aactgaccaa gtcagtaacc tagacttagg gcaaggcggc
      661 actgacctag tttgaagaga tttccgaaga gtataaattt taatattttc ttgtgttatt
      721 ccttgacaat tcaatttgga aaatatatga taaagataat gacagcggtg tcattctatc
      781 tattttaaga aaagtaataa tcaattgtta aaaatagtaa aaaaattgga ggttctgatg
      841 aaatattttg ttcctaatga ggtattcagt attcgtaaat taaaggtggg gacttgctcg
      901 gtactattgg caatttcaat tttgggaagc caaggtattt tatcggatga agttgttact
      961 agttcttcac cgatggctac aaaagagtct tctaatgcaa ttactaatga tttagataat
     1021 tcaccaactg ttaatcagaa tcgttctgct gaaatgattg cctctaattc aaccactaat
     1081 ggtttagata attcgttaag tgttaatagt atcagctcta atggtactat tcgttccaat
     1141 tcacaattag acaacagaac agttgaatct acagtaacat ctactaatga aaataagagt
     1201 tataaggaag atgttataag tgacagaatt atcaaaaaag aatttgaaga tactgcttta
     1261 agtgtaaaag attatggtgc agtaggtgat gggattcatg atgatcgaca agcaattcaa
     1321 gatgcaatag atgctgcagc tcaagggcta ggtggaggaa atgtatattt tccggaagga
     1381 acttatttag taaaagaaat tgttttttta aaaagtcata cacacttaga attgaatgag
     1441 aaagctacca ttctaaatgg tataaatatt aagaatcacc cttctattgt ttttatgaca
     1501 ggtttattta cggatgatgg tgcgcaagta gaatggggcc caacagaaga tattagttat
     1561 tctggtggta ctattgatat gaacggtgct ttgaatgaag aaggaactaa agcaaaaaat
     1621 ctaccactta taaattcttc aggtgcattt gctattggga attcaaataa cgtaactata
     1681 aaaaatgtaa cattcaagga tagttatcaa gggcatgcta ttcaaattgc aggttcgaaa
     1741 aatgtattag ttgataattc tcgttttctt gggcaagcct tacccaaaac gataaaggat
     1801 gggcaaatca taagtaagga gagcattcag attgaaccat taactagaaa aggttttcct
     1861 tatgccttga atgatgatgg gaaaaaatct gaaaatgtga ctattcaaaa ttcctatttt
     1921 ggcaaaagtg ataaatctgg ggaattagta acagcaattg gcacacacta tcaaacattg
     1981 tcgacacaga acccctctaa tattaaaatt ttaaataatc attttgataa catgatgtat
     2041 gcaggtgtac gttttacagg attcactgat gtattaatca aaggaaatcg ctttgataag
     2101 aaagttaaag gagagagtgt acattatcga gaaagcggag cagctttagt aaatgcttat
     2161 agctataaaa acactaaaga cctattagat ttaaataaac aggtggttat cgccgaaaat
     2221 atatttaata ttgccgatcc taaaacaaaa gcgatacgag ttgcaaaaga tagtgcagaa
     2281 tatttaggaa aagtatcaga tattactgta acaaaaaatg taattaataa taattctaag
     2341 gaaacagaac aaccaaatat tgaattatta cgagttagtg ataatttagt agtctcagag
     2401 aatagtatat tcggtggtaa agaaggaatt gttattgagg attcaaaggg taaaataacc
     2461 gttttaaata accaatttta taatttatcc ggtaagtata tatcattcat caaatctaat
     2521 ggaaatggga aagaaactgt tatacgtgat agcgatggta atttcaatat tgtaacggag
     2581 aatgggcttt acaaaattgt aacaaataat ttaagtgata aaaacgaaaa agaaaaaaac
     2641 aaagaggaaa aacaatctaa ttcaaataat gtaattgata gtaaccagaa gaacggagag
     2701 tttaactcaa gtaaagataa tatacaaatt aatgacaaga tcgacaataa acaagataat
     2761 aagacagaag aagtgaacca taaaatagtt ggagatggca tagaaactga aaatcatatt
     2821 aataaatcta aagaaatagt agatgtaaaa caaaaattac caaagactgg ttcgaacaag
     2881 attatggaac tattcttaac agtgacagga attagtttac ttttgacact aaaagggttg
     2941 aagtattatg gtaaagataa ataaaatttg ttcgatacaa ggaagttctg tagaaaatga
     3001 ggatactgta ggttcacaaa atcagtattt ttggattatt gatggagcta cagatttata
     3061 taattctaaa gaaggagata ggttattcag tctcagaagt tgtacatatt ttatcagaaa
     3121 gtttgtcggt taattgtaaa gaatcaaaaa ctcttaaaca aatatttgaa actgctttac
     3181 tcgaggttaa agatgaaatc ggtttaaact catatgaatt aacagagtat tatcgcttac
     3241 caacatttgc ttttatattt gctaaacttt cagaaaaaaa gttggagtat atgatgctag
     3301 gtgactgcgt catgttagta aacgaaatgg aaataactga tcatagagta gataacttat
     3361 ttgaaaaagg gaaaaatgag ataaaagact caattggtac aaatagtgtt ttaaataaaa
     3421 aaattatttt acaaaaaatc agaaaattat ctaatcaacc gagtgggtac tggataggtt
     3481 cgttggatga gagattttta gaccatgcta ttattaatca aatagatgtt actagtgaac
     3541 aaattgtttt aatgagcgat ggcttttatg aattctatca aaataatcaa aataaaactt
     3601 ttgaagagtt aattaagatg agatttaatt cttctgctat tgatccaatc tatggaaaga
     3661 aagatgatgc aagtattcta gttattgat
//
LOCUS       DAVKLX010000090         3296 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKLX010000090 DAVKLX010000000
VERSION     DAVKLX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.090"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(169..702)
                     /locus_tag="VW325_002056"
     CDS             complement(169..702)
                     /locus_tag="VW325_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9735370.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(791..1567)
                     /gene="recX"
                     /locus_tag="VW325_002057"
     CDS             complement(791..1567)
                     /gene="recX"
                     /locus_tag="VW325_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9735371.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1605..2960
                     /gene="rlmD"
                     /locus_tag="VW325_002058"
     CDS             1605..2960
                     /gene="rlmD"
                     /locus_tag="VW325_002058"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9735372.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            3022..3093
                     /locus_tag="VW325_002059"
     tRNA            3022..3093
                     /locus_tag="VW325_002059"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3055..3057,aa:Glu,seq:ttc)
BASE COUNT         1057 a          584 c          616 g         1039 t
ORIGIN      
        1 aatagtatat ctatttgttt tatatatttt acgaattctg cataaatctc tttctagtaa
       61 tgtgttgtaa ctctgctata atggatttat tcctttttgt atttacacaa tttattttat
      121 actaccaaaa aaacaaggtc aggattttgt tcctgacctt tgacaacttt accgattctt
      181 tagttctaca tagcgcttgt accaaatgtt tacataggct tctgagaaag gaccacgtcc
      241 attgttaatc caatcaacaa gaattttgac atgttctttt aaaatatagt ccaagtcatc
      301 agaataattc attttgcgtt tgtgacgctc gtattcttca acgtccaaga gacgtttttc
      361 cccatctgta aaaattttaa catccaaatc gtaatcaata tacttcagtg cttcttcatc
      421 cagatagtag gggctagcca tattgcaata gtaagaagtt ccattatcac gaatcatggc
      481 aatgatatta aaccaatatt tcttgtgaaa gtaaacaata gccggttctc gagtgaccca
      541 acgacgacca tcactttcgg taacaagtgt atgatcgttg acaccaataa tggcgttttc
      601 tgttgttttt agtaccatgg tgtcccgcca agttcggtgg agactcccat catgcttata
      661 actttgaatt gtaataaagt cgccttcttt tggaagcttc ataactaacc aactttctac
      721 aatttataag tttatcattt actattgtac cataaaatta cctaaaatct gtgaatttca
      781 cttggaaata ttaaagatat tctctaagag cgcttgctat atccgaaaaa tcgtagccct
      841 ttcgtgctaa aacttgagtt aaacgctgct tcagttcgta tccttcatac tttcgagcat
      901 acttagtata ttgcttatca agttccttga agatgagttc ttgagtcgtt tcttggtcaa
      961 cttggctatc taagtcgtca aaggcaattt tagcatcaga ataagagaag cccttgttag
     1021 tcaagttctg gataatctta tcttgcaggg cacgagctgg aagttttccc tcatattttt
     1081 tcaatagttt attggctaca cgttgagcaa cttcagaaaa atcaaattct ttcaagttct
     1141 cttctatagt agattttgaa atcccttttt gagatagttt ctgagtcagc acataaggac
     1201 ccttgtctcc tgaaagttga ttggtattga tgatagcata agcgtactga ccatcattaa
     1261 tccacttatc ttctttaaga ttagcaataa cttgagaaac gatgttttta tcaatatcgt
     1321 attttttcag atattctctg acttcttttt cagtgcgtgc tttaaaggat aagtggtaga
     1381 gagcgagatt cttaccataa gaaaattgag caaagtcttg aatctctttc aattcctctt
     1441 cgcttatcac cttatctctc gataacataa aacgaacaat tgtatcttcg gtgatatagc
     1501 atttgtcgcc attatcaagc tccatcaggt agagtctttt tttcttttca agttttgtga
     1561 ttttcatagc tctattataa ctcaaaatgt gataagatag gggtatgaat ctgaaagtga
     1621 aacaaaaaat accattaaaa atcaagcgca tgggaattaa cggtgaggga atcggctttt
     1681 accaaaaaac attagtcttt gtaccaggag ctctcaaagg cgaagatatc tattgtcaga
     1741 ttacttctat tagacgcaac tttgttgaag caaaattact gaaggtcaac aagaagtcta
     1801 aatttcgaat tgtgccatct tgtactattt ataatgaatg cggaggctgc caaatcatgc
     1861 acctgcatta tgataagcag ctggagttca agacggactt acttcatcaa gcgctgaaaa
     1921 aatttgctcc tgcaggatat gaaaattatg aaattcgtcc aactattgga atgcaggaac
     1981 caaaatatta cagagctaag ttacaatttc agactcgaaa atttaaaaat caggtcaagg
     2041 cgggcttata tgcacaaaac tctcactatt tagtagagtt gaaagactgc ctggtacaag
     2101 ataaggaaac ccaagtgatt gctaatcgct tagcagaatt acttacttat caccagattc
     2161 caatcacgga tgagagaaaa gttctaggtg tccgtactat tatggtccga cgcgcgagaa
     2221 agaccggaca ggttcagatt attattgtta caaaccgcca gcttaattta actcaattgg
     2281 taaaagagtt ggttaaagat ttcccagaag ttgtgacagt agctgttaat acaaatacag
     2341 ctaaaaccag tgagatatat ggtgaaaaga cagagattat ctgggggcaa gagagtattc
     2401 aagaaggtgt actcaattat gaattttcac tatcccctcg agctttttat caactaaatc
     2461 ctgagcaaac agaagtcctc tatagcgaag cagtaaaagc gctggatgtt gataaagaag
     2521 accatttgat tgacgcttat tgtggagttg gaacgattgg atttgccttt gcaaagaaag
     2581 ttaaaacact cagaggtatg gatattattc cagaagctat tgaagatgcc aagcgaaatg
     2641 ctaaaagaat gggatttgac aatactcatt atgaagctgg aacggcagaa gagattattc
     2701 ctcgttggta caaggaaggc taccgagcag atgctttgat tgttgaccca ccacgtacag
     2761 gtctggatga taagttatta gatactattc ttacttatgt accagaaaaa atggtttata
     2821 tttcttgtaa tgtttcgacc ttggctcgtg atttggtacg cttagtagaa gtctatgatc
     2881 ttcattatat ccagtcggtc gatatgttcc cacatacagc tcgaactgaa gctgttgtaa
     2941 aattaataaa aaaagtttaa aaaagtagtt gacaaagttt gaaaaggctg tataatagta
     3001 agagttgaaa ataacaactc aggtccgttg gtcaaggggt taagacaccg ccttttcacg
     3061 gcggtaacac gggttcgaat cccgtacgga ctatggtatg ttgcgtcagg accacttgat
     3121 gaaaaaaagt ttaaaaaaag cttaaaaatc ttcaaaaaag tgttgacaag tgaaagtggc
     3181 tgtgatatac taatatagtt atcgcttgag agaagcaagt gacaaagacc tttgaaaact
     3241 gaacaagacg aaccaatgtg cagggcgcta caacataagt tgtagtactg aacaat
//
LOCUS       DAVKLX010000091         3063 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKLX010000091 DAVKLX010000000
VERSION     DAVKLX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.091"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            225..1511
                     /locus_tag="VW325_002060"
     CDS             225..1511
                     /locus_tag="VW325_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9735373.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKGRKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDATQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQAGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            1526..1837
                     /locus_tag="VW325_002061"
     CDS             1526..1837
                     /locus_tag="VW325_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735374.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            2083..2811
                     /locus_tag="VW325_002062"
                     /pseudo
     CDS             2083..2811
                     /locus_tag="VW325_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT          950 a          530 c          663 g          920 t
ORIGIN      
        1 atgtattttt acgagtcagt atctttggat actggttttt acttttctag actttttgac
       61 tatttgttaa aactgggata attttcgact gtttaacagt tattatgcaa agtctaaaag
      121 attagaattg tcaaaacaat ccgtctaggc ttgattttat cctttattta ctataaaatg
      181 agaaggaaaa atgtcaaact tttatattgc aaataggaga aatcatgaca aaaacattaa
      241 aacgtcctga ggttttatca cctgcaggga ctttagagaa gctaaaggta gctgttcagt
      301 atggagcaga tgctgtcttt atcggtggtc aggcctatgg tcttcgtagc cgtgcgggaa
      361 actttacttt cgaacagatg gaagaaggcg tgcagtttgc ggccaagtat ggtgccaagg
      421 tctatgtagc ggctaatatg gttatgcacg aaggaaatga agctggtgct ggtgagtggt
      481 tccgtaaact gcgtgatatc gggattgcag cagttatcgt atctgaccca gccttgatta
      541 tgattgcagc gactgaagca ccaggccttg aaatccacct ttctacccaa gccagtgcca
      601 ctaactatga aacccttgag ttctggaaag agctaggctt gactcgtgtc gttttagcgc
      661 gtgaggtttc aatggaagaa ttagctgaga tccgcaaacg tacagatgtt gaaattgaag
      721 cctttgtcca tggagctatg tgtatttcat actctggacg ttgtactctt tcaaaccaca
      781 tgagtatgcg tgatgccaac cgtggtggat gttctcagtc atgccgttgg aaatacgacc
      841 tttacgatat gccatttggg aaaggacgta agagtttgca gggtgagatt ccagaagaat
      901 tttcaatgtc agccgttgac atgtctatga ttgaccacat tccagatatg attgaaaatg
      961 gtgtggacag tctaaaaatc gaaggacgta tgaagtctat tcactacgta tcaacagtaa
     1021 ccaactgcta caaggcggct gtggatgcct atcttgaaag tcctgaaaag tttgaagcta
     1081 tcaaacaaga cttagtggac gagatgtgga aggttgccca acgtgaattg gcaacaggtt
     1141 tctactacgg tataccatct gaaaatgagc agttgtttgg tgctcgccgt aaaatccctg
     1201 agtacaagtt tgtcgctgaa gtggtttctt atgatgatgc gacacaaaca gcaaccattc
     1261 gtcaacgaaa cgtcattaac gaaggggacc aagttgagtt ttatggtcca ggtttccgtc
     1321 attttgaaac ctatattgaa gatttgcatg atgccaaagg caataaaatc gaccgcgctc
     1381 caaatccaat ggaactattg actattaaag tcccacaacc cgttcaagct ggagacatgg
     1441 ttcgtgcatt aaaagaagga ctcatcaatt tttataagga agatggaacc agcgtcacag
     1501 ttcgagctta agaaaggaaa aggaaatgat agaggcacag ggtttcttag tggataagca
     1561 aacaagatgc attcattacc atagcaagct ggatattatt gctttacaat gctatgattg
     1621 taaaaagtat tatgcttgtt atcggtgtca tgattcatta gaaaatcacc cttttgagcc
     1681 gtatccctta tctttgatac aggataagcc tattttatgt ggtgtttgtc taaaactatt
     1741 aacatataag caatataaag aaagtttaag ttgccccttt tgtttttctc gctttaatcc
     1801 aggttgccaa aatcataagg aacgctattt taaatagcaa atcatctagt tttgaagtag
     1861 gagaaaactc aattccaaga gaaaatgaag taaatcttcc cacaataaaa cgcataatat
     1921 caagattgtt caatacctga tactatgcgt ttttaagatt ttaaagactt ttttccttta
     1981 tctggtattt tgactacttg ttaaaactgg gttaattttc aactgtttaa cagttattat
     2041 gcaaagtcta aaagattaga attgtcaaaa caatccgtct agagtatgcg tgatgccaac
     2101 cgtggtggat gttctcagtc atgccgttgg aagtacgacc tttacgatat gccatttggg
     2161 aaagaacgta agagtttgca gggtgagatt ccagaagaat tttcaatgtc agccgttgac
     2221 atgtctatga ttgaccacat tccagatatg attgaaaatg gtgtggacag tctaaaaatc
     2281 gaaggacgta tggagtctat tcactatgta ttaacagtaa ccatctgcta caaggcggct
     2341 gtggatgcct atcttgaaag tcctgaaaag tttgaagcta tcaaacaaga cttggtggac
     2401 gagatgtgga aggttgccca acgtgaactg gctacaggat tttactatgg tacaccatct
     2461 gaaaatgagc agttgtttgg tgctcgtcgt aaaattcctg agtacaagtt tgtcgctgaa
     2521 gtggtttctt atgatgatgc ggcacaaaca gcaactattc gtcaacgaaa tgtcattaac
     2581 gaaggggacc aagttgagtt ttatggtcca ggtttccgtc attttgaaac ctatattgaa
     2641 gatttgcatg atgccaaagg caataaaatc gaccgcgctc caaatccaat ggaactattg
     2701 actattaaag tcccacaacc tgttcaatca ggagacatgg ttcgagctct taaagagggg
     2761 cttatcaatc tttataagga agatggaacc agcgtcacag ttcgtgctta atgtagttgt
     2821 ttagttttaa aaaattatgc aaagctccat atacaacatt taaacgagat taaagaatgg
     2881 cgaaatccct tgatgcgcaa gagattagct gtcctttttt attttttaag tgataaagtc
     2941 ggagcttagg tatcaaagcc tatcaaatta aacaaagaag cgatgtctta gatattttga
     3001 aaaaaattaa taagcagaaa actctctatt attttgttgt agagagtttt ttattaataa
     3061 aat
//
LOCUS       DAVKLX010000092         2906 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKLX010000092 DAVKLX010000000
VERSION     DAVKLX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.092"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..475)
                     /locus_tag="VW325_002063"
     CDS             complement(<1..475)
                     /locus_tag="VW325_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735375.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLN
                     MNRNGCMGDFPHDFFDIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRLF"
     gene            complement(489..1202)
                     /locus_tag="VW325_002064"
     CDS             complement(489..1202)
                     /locus_tag="VW325_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9735376.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1662..2042)
                     /locus_tag="VW325_002065"
     CDS             complement(1662..2042)
                     /locus_tag="VW325_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU9735377.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQTQLAELEINK"
     gene            complement(2459..>2906)
                     /locus_tag="VW325_002066"
     CDS             complement(2459..>2906)
                     /locus_tag="VW325_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9735378.1"
                     /translation="RNKNGQADTNQTEKPNEEKPQTEKPEEDKEHDEVSEPTHPESDE
                     KENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEK
                     VTDSSIRQNAVETLTGLKSSLLLGTKDNNTISAEVDSLLALLKESK"
BASE COUNT          905 a          571 c          450 g          980 t
ORIGIN      
        1 aaaacaatcg aaaatacaat gcattctctt ggtgtaaaat cgctcttttt aaaggataat
       61 actctttaat attattaact ttttgttcgt aggcatattt tgctacatga tctaggtata
      121 tatcaaaaaa gtcatgagga aaatctccca tacagccatt acgattcata ttgaggctat
      181 attttcgatt agtaagataa ttcggaaata tatttcctat tactctaaga ttatctactt
      241 gtgaatccct tctcccaata taatcccctt ctttgactaa tgatggagat ttccaagtaa
      301 aaattaaatc gccagagaaa tttttcttat caaaaaactt aaaaagagat tgataaatac
      361 tatttgctaa ttgacaagtg tcagatgtaa attcataatt aacagcttca ttaaaatcaa
      421 cttcagacat ttcaatgtac tcttcaatag taatattagc tttttttgta gtcattagct
      481 aatctccttt aaaatgtgat cttttcaaga atatttttat ctaaacaatc cagcaagtct
      541 tggtaagaat agacttcgta agtcggctgg gcttgtgtgt gattttcgag gtgatgagga
      601 ttataccaga tagtgtcaat ccccgcatta ttgccacctt gaatgtcggc ggttagagaa
      661 tctccaatca tcagcgtctt ttctttacta aatccagcaa tttgctggcc aatcttttca
      721 taaaagagag catccggctt ttgagtttgc aactgttctg agataaagac ttgattgaaa
      781 taaggtgcta gaccagattg agccaaacgt cctgtctgaa tggcagtaat gccatttgtc
      841 gcagcataca agtcataatc acgctcaatg aggctgtcca agagatcatg agcgcccgat
      901 agtgtttgtc cctgctgggc gaggtaaaat tggtaacgct gggcaagaaa actaccgtct
      961 ttttcctgtc caaaatgaga aaataaacga gaaaagcgcg tgttaaccag ctcttgttta
     1021 ctgattttct tcagctccaa gtctttccag agagccttgt tcataggaac gtaataatct
     1081 ttataagcct gaatatccgc aactccttct tcttttagaa gttgggtcaa agccacatcc
     1141 tcagcagcat caaaatcaag aagagtgtgg tcgaggtcga agagtagaaa tttgtagaac
     1201 aatttgaggt tttcctttct gaaaattcat taagaacatt atatcataaa gcacctcata
     1261 caattaacta atttaatcac ttaaaaaaaa ttcgaacact ttctatacaa ctgacagctc
     1321 aaatctttca gaatagaaca atactaacta tcgagcaccc ccgtcttcat aaatatatgt
     1381 gtaattctag acctagaatt cctataaact aaatgctttc atactcttcc aagtaattga
     1441 ttgccttaaa ttttaatttt tgaaggattc taaagctaga atagccccat cacaatcagt
     1501 tttgattgat tcacaattta gaaacactat agtttcactc ctgttaaaat aaaaaggaac
     1561 tgcataaagc aatccctttc taattttgaa atcatttact taacatttta tagttgagat
     1621 aatcaatagc ttacctataa aaagagttat agtaaaattc cttatttatt gatttcaagc
     1681 tccgctaact gtgtttgaat aactgacagt tctgcaccag cctgcagaag agcagctgca
     1741 ttataggcac cttctacaat tggaaccctg ttgatgatga tacttttatc actgaaatca
     1801 gtcaccattt ttaagttcat tttagcagaa cctaggtcaa aaaaggcaag taaagtatct
     1861 gctggatttt cggaaacaac cctatctact tgatcaaaac tcgttccaat tcctccgccc
     1921 tcggttcctc ctacataagt aatcggaaca tctttagcta ctttactaat cagttcaaca
     1981 acaccttctg caatgtgttt ggaatgtgaa acgataacaa gaccaatacc aatactttcc
     2041 atcaaaccac cccagtttct aaaatagcag taaagagtaa tcctgatgag aatgatccag
     2101 gatcaatatg tccaagaaac catatgctcc taagacaaga gccaacagac tggtcatcaa
     2161 taatagtatt gttctttttt tcatcattac tccttaacta gtgtttaact gattaattag
     2221 ccagtaaata gtttatcttt atttacacta tctgttaaga tatagtaaaa taagaacagg
     2281 acagtcaaat cgatttctaa caatgtttta gaagtagagg tatactattc tagtttcaat
     2341 ctactatatt ttgcacattt tcatgaaaaa aatgagaact agaactcaca ttctgctctc
     2401 atttttcgtt ttcccgttct cctatcctgt ttttaggagt tagaaaatgc tgctaccttt
     2461 acttactctc ctttaataaa gccaagagac tatctacttc tgctgaaata gtgttattat
     2521 ctttcgttcc gagaagaaga ctacttttta gaccagtcaa tgtctccaca gcattttgtc
     2581 taatactaga atctgttact ttttctagca aggcctccgc atctgctatc ttagcgttaa
     2641 taacagaatg ctctacttga ggaatttcag cctcatctgt agtatcttca gcttcttcct
     2701 ctgtctcttc cgtatcagtg cttggtttat aaagattatc tgctgaagga tttaaaccag
     2761 cgtgattctc tttttcatca gattcagggt gagttggctc acttacttca tcatgttcct
     2821 tatcttcctc aggtttttct gtctgaggtt tctcctcgtt tggtttttcc gtttgattgg
     2881 tatcagcttg accatttttg tttctt
//
LOCUS       DAVKLX010000093         2616 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKLX010000093 DAVKLX010000000
VERSION     DAVKLX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.093"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            29..1375
                     /gene="gdhA"
                     /locus_tag="VW325_002067"
     CDS             29..1375
                     /gene="gdhA"
                     /locus_tag="VW325_002067"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9735379.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV"
     gene            complement(1664..>2616)
                     /locus_tag="VW325_002068"
     CDS             complement(1664..>2616)
                     /locus_tag="VW325_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735380.1"
                     /translation="FLENIEESSMQSVIVSSPESEKYEWQLAYLTQLENVKNEDLQTL
                     KSILEANSLPCYFTILNFERLILKDPSLKELLIQKAGNTNFVISDFIREEEVPKLINL
                     IGVKELKFWYLINLENCQNHSYNLFQKLGEKDVDFSVEVLKKIDELRIGHSNLGYMVL
                     HSISEFRDKKEIYKKFIRFAINRPYYYYNNMIDDIIKNDSQIILEILEETNNEQSAIR
                     LVNLGVEFLENNNQKLILFNLLRAKGFGKKSFQEIHFTPYSHFYTGSHVPVLELEKEL
                     LERIKKIFETGIDYINLLLYLNKLIDCKRKAIERELEKEF"
BASE COUNT          830 a          481 c          436 g          869 t
ORIGIN      
        1 taaaatatga caataaaagg agaacgatat gacatctgct aaagaatata tccaaagcgt
       61 gtttgaaact gtaaaagctc gtaacgggca cgaggctgaa ttcctccaag ctgttgaaga
      121 atttttcaac actttggaac ctgtatttga aaaacaccct gagtatatcg aagaaaatat
      181 cttggcacgt attactgagc ctgagcgcgt gatttctttc cgtgttcctt gggttgaccg
      241 tgatggaaaa gttcaagtaa accgcggcta ccgtgttcaa ttcaactcag ctgttggacc
      301 atataaaggc ggacttcgtt tccacccaac tgtaaaccaa gggattttga aattcctcgg
      361 atttgaacaa atctttaaaa acgtcttgac tggacttcct atcggtggag gtaaaggtgg
      421 atcagacttc gatcctaaag gtaaaacaga tgctgaagtg atgcgcttct gccaaagctt
      481 catgactgaa ttgcaaaaac acattggacc atcacttgac gtacctgctg gtgatatcgg
      541 tgttggtgga cgtgagattg gttaccttta cggtcaatac aagcgtctta accaatttga
      601 tgctggtgtc ttgactggta aacctcttgg atttggtggt agcttgattc gtccagaagc
      661 aactggttac ggtttggttt actatactga agaaatgctc aaagctaacg gtaacagctt
      721 tgctggtaag aaagtggtta tttcaggttc tggtaacgtt gctcaatacg ctcttcaaaa
      781 agcaaccgaa cttggtgcaa cagttatctc tgtttctgac tcaaacggct atgttatcga
      841 tgaaaacggt atcgacttcg atcttttggt tgatgttaaa gaaaaacgtc gcgctcgttt
      901 gactgagtat gcagctgaga aagcaactgc tacttatcat gaaggttctg tatggactta
      961 cgctggaaac tatgacattg cgcttccatg tgctactcaa aacgaaatca acggtgaagc
     1021 agctaaacgt ttggttgctc aaggcgttat ctgtgtatct gaaggtgcca acatgccaag
     1081 caaccttgat gccatcaaag tttacaaaga aaatggtatc ttctacggac ctgccaaagc
     1141 tgccaacgct ggtggtgtag ccgtttcagc ccttgaaatg agccaaaaca gccttcgcct
     1201 ctcatggact cgtgaagaag ttgatggacg tctcaaagac atcatgacca acatctttaa
     1261 cacagctaaa acaacttcag aaacatatgg tcttgataaa gactaccttg caggagctaa
     1321 cattgctgcc tttgaaaatg tagcaaacgc tatgattgca caaggcgtgg tataaaaaaa
     1381 aattacggaa actgactacc cagacgggta gccagttttt tcaattccta aaatttaaga
     1441 ttctaatacc ctttatgcaa aaatatagaa ttctaccttt ttctttatat aggctaatgt
     1501 ttttaatata tttattgtct atttttgaaa agacaataaa actattctac tatagattat
     1561 catttcccac aattcataac tcaaacctaa tattttgtta taaaacagta gattttattt
     1621 attatatctg gcaccttaaa aagtaagaaa tataaattaa aatttaaaac tctttttcca
     1681 attctctttc aatagctttt cgcttgcaat caattagttt attcaagtac agtagcaagt
     1741 taatgtagtc tatacccgtt tcaaatatct ttttaattct ttctaggagt tctttttcta
     1801 attccaacac cggaacatga ctaccagtat aaaaatgtga atagggagta aaatgtattt
     1861 cttggaatga cttcttacca aatcctttag ctctgagaag attaaatagt attagttttt
     1921 gattgttgtt ttctaaaaat tctacaccta gattgaccaa tctaattgca gattgctcat
     1981 tatttgtttc ctccagtatt tctaatatga tctgactgtc attttttata atatcatcaa
     2041 tcatattatt gtagtagtaa tatggcctat ttattgcaaa acggataaac tttttataaa
     2101 tttctttctt atctctaaat tcacttatag aatgtaatac catgtatcct aaattggaat
     2161 gacctattct caattcatca attttcttca atacttctac cgaaaaatca acatcttttt
     2221 cacctaattt ttgaaaaaga ttatagctgt gattctgaca attttctaaa tttattaaat
     2281 accaaaactt caactctttt actccaatta gatttattaa ttttggcacc tcttcttctc
     2341 ttataaaatc agaaataaca aaatttgtat tcccagcttt ttgtattaac aactctttta
     2401 aactaggatc tttcaaaatc aatctctcaa aatttaaaat tgtaaaataa catggtaaag
     2461 aattagcttc tagaatactc tttaatgttt gtagatcttc atttttaacg ttttcaagtt
     2521 gtgttaaata agcaagttgc cattcatatt tttctgattc tggagaggaa acaataacag
     2581 actgcattga actttcctca atattttcta aaaaac
//
LOCUS       DAVKLX010000094         2409 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKLX010000094 DAVKLX010000000
VERSION     DAVKLX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.094"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            144..1340
                     /gene="tuf"
                     /locus_tag="VW325_002069"
     CDS             144..1340
                     /gene="tuf"
                     /locus_tag="VW325_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9735381.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(1449..2409)
                     /locus_tag="VW325_002070"
                     /pseudo
     CDS             complement(1449..2409)
                     /locus_tag="VW325_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          751 a          467 c          501 g          690 t
ORIGIN      
        1 ttaaattttt accaagaata attcacaaaa acgttgtaaa acacttgcaa tttagctgaa
       61 atttgataaa atagtaagga aagttagact gtattgccta ctgtctatct ataaaatata
      121 ttttattgga ggcttttact caaatggcaa aagaaaaata cgatcgtagt aaaccacacg
      181 ttaacattgg tactatcgga cacgttgacc acggtaaaac taccctaact gcagctatca
      241 caactgtttt ggcacgtcgc ttgccttcat cagttaacca acctaaagac tatgcgtcta
      301 tcgatgctgc tccagaagaa cgcgaacgcg gtatcactat caacactgcg cacgttgagt
      361 acgaaactga aaaacgtcac tacgctcaca tcgacgctcc aggacacgcg gactacgtta
      421 aaaacatgat cactggtgct gctcaaatgg acggagctat ccttgtagta gcttcaactg
      481 acggaccaat gccacaaact cgtgagcaca tccttctttc acgtcaggtt ggtgttaaac
      541 accttatcgt cttcatgaac aaagttgact tggttgacga cgaagaattg cttgaattgg
      601 ttgaaatgga aatccgtgac ctattgtcag aatacgactt cccaggtgac gatcttccag
      661 ttatccaagg ttcagcactt aaagctcttg aaggtgactc taaatacgaa gacatcgtta
      721 tggaattgat gaacacagtt gatgagtata tcccagaacc agaacgtgac actgacaaac
      781 cattgcttct tccagtcgag gacgtattct caatcactgg acgtggtaca gttgcttcag
      841 gacgtatcga ccgtggtatc gttaaagtca acgacgaaat cgaaatcgtt ggtatcaaag
      901 aagaaactca aaaagcagtt gttactggtg ttgaaatgtt ccgtaaacaa cttgacgaag
      961 gtcttgctgg agataacgta ggtgtccttc ttcgtggtgt tcaacgtgat gaaatcgaac
     1021 gtggacaagt tatcgctaaa ccaggttcaa tcaatccaca cactaaattc aaaggtgaag
     1081 tctacatcct tactaaagaa gaaggtggac gtcacactcc attcttcaac aactaccgtc
     1141 cacaattcta cttccgtact actgacgtta caggttcaat cgaacttcca gcaggtactg
     1201 aaatggtaat gcctggtgat aacgtgacaa tcgacgttga gttgattcat ccaatcgccg
     1261 tagaacaagg tactacattc tctatccgtg agggtggacg tactgttggt tcaggtatgg
     1321 ttacagaaat cgaagcttaa ttcgatttag ttcccagaag aacaattatt taagtcagac
     1381 actaaaagaa tcttgcttgg caaggttctt ttttggctct ttgtcaactg tagtgggttg
     1441 aaaaaaaact aagctcgaga aaggacaaat ttcgtccttt cttttttgat gttcagggcg
     1501 atgaaaatcc gttttttgaa gttttcaaag ttccgaaaac caaaggcatt gcgcttgata
     1561 agtttgatga gattattggt cgcttccaat ttggcgtttg aatagggtag ttgaagggcg
     1621 ttgacgattt tctttttgtc ctttagaaag gttttaaaga cagtctgaaa aagaggatga
     1681 acctgcttaa gattgtcctc aatgagtccg aaaaatttct ccggttcctt attctgaaag
     1741 tgaaacagca agagttgata gagctgatag tggtgtttca agtcttccga ataactcaaa
     1801 agcttgttta aaatctcttt attggttaag tgcatacgaa aagtaggacg ataaaatcgc
     1861 ttatcactca gtttacgact atcctgttgt atgagcttcc agtagcgctt gatagccttg
     1921 tattcatgag actttcgatg aaactgattc atgatttgga cacgcacacg actcatagta
     1981 cggctaagat gttgtacaat gtgaaagcga tccaacacga ttttagcatt cgggagtgaa
     2041 acagtctggg agactgtttc agcctgagcc tagaaatttg aaagcgaagc tgtttagcta
     2101 agtcatagta aggactaaac atatccatag taataatttt gacgcgacat cggacggctc
     2161 tatcatattt aagaaagtga tttcgaatga tagcttgtgt tcttccctca agaacagtga
     2221 tgatattgag attgttaaaa tcttgcgcaa tgaagctcat ctttcccttt gtaaaagcat
     2281 actcatccca agacataatc tcaggaagac aagaaaaatc atgtttaaag tgaaagtcat
     2341 tgagcttacg aataacagtt gaagttgaga tggaaagctg atgggcaata tcagtcatag
     2401 aaatctttt
//
LOCUS       DAVKLX010000095         2229 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKLX010000095 DAVKLX010000000
VERSION     DAVKLX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.095"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..>2229)
                     /locus_tag="VW325_002071"
     CDS             complement(<1..>2229)
                     /locus_tag="VW325_002071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735382.1"
                     /translation="LVDADSEADVLAEADALVDADSDALVDAEAEALVDAEADALVLA
                     EAEALIDAEAEALVDAEAEALVDAEAEALVDAEAEALVLAEADALVDADSDAEILAEA
                     DALVDAEAEALVLAEADALVEAEDEALVLAEADALVLAEADALVDADSDADVLAEDEA
                     LVDAEAEALVLAEAEALVDADSEALVDAETEALVDAEAEALVDADSDADVLAEADALV
                     DADSDALVDAEAEALVDAEAEALVLAEADALVDAEAEALVLAEADALVEAEAEALVLA
                     EADALVDAEADALVLAEAEALVDAEAEALVDAEAEALVDAEAEALVLAEAEALVDADS
                     EALVDAETEVLVEAEAEALVEAEAEALVDAEADALVDAEADALVDAEAEALVDAEAEA
                     LVDAEAEALVLAEADALVDADSEADVLAEAEALIDAEADALVDAEAEALVDADSDALV
                     LAEAEALVDAEAEALVEADSDAEILAEADALVDAEAEALVDAEAEALVDADSEALVDA
                     EAEALVDAEAEALVLAEAEALVDAEADALVLAEAEALVLAEAEALVLAEAEALVDAEV
                     DALVEAEADALVLAEADALVDAEAEALVDADSDALVDAEAEALVLAEADALVDAETDA
                     LVLAEAEALVDADSDALVLAEAEALVDADSDALVLAEAEALVDAEADALVLAEAEALV
                     DAEAEALVDAEADALVLAEADALVLAETDALVDAEADALVDAEADALVDAEADALVLA
                     EA"
BASE COUNT          544 a          744 c          499 g          442 t
ORIGIN      
        1 cggcttcagc aagcaccagt gcgtcggctt cagcgtcaac cagtgcatca gcttcagcat
       61 caacgagtgc atcggcttca gcgtcaacca gtgcatcagt ctcagcaagc accagtgcgt
      121 cggcttcagc aagcaccagt gcgtcggctt ccgcatcaac aagtgcttca gcctcagcat
      181 cgacaagcgc ttcagcttca gcaagcacca gtgcatcagc ctcagcatcg acaagcgctt
      241 cggcttccgc aagtacaagt gcgtctgagt cagcatcgac aagcgcttcg gcttccgcaa
      301 gtacaagtgc gtctgagtca gcatcaacaa gtgcttcggc ttccgcaagt acaagtgcgt
      361 cagtctcagc gtcgacaagt gcgtcggctt cagcaagcac aagcgcctca gcttcagcgt
      421 caacgagtgc gtctgagtca gcatcgacaa gcgcttcagc ttcagcatca acgagtgcat
      481 cagcctcagc aagtacaagc gcgtcagctt cagcctcaac gagcgcatca acctcagcat
      541 caaccagtgc ctcagcttca gcaagtacta gtgcctcagc ttcagcaagc accagtgcct
      601 cagcttcagc aagcaccagc gcgtcagctt ccgcatcaac aagcgcctca gcctctgcaa
      661 gtaccagtgc ttcggcctca gcatcgacaa gcgcctcggc ctcagcgtca acaagtgcct
      721 ctgaatcggc atcaaccagt gcttcagcct cagcatcgac aagcgcttca gcttcagcgt
      781 cgacaagtgc gtcagcttca gcaagtatct cagcgtctga atcggcctca acaagcgcct
      841 cggcttcagc atcaacgagt gcttcagctt cagcaagtac cagtgcatct gagtcagcat
      901 caaccagtgc ctcagcttca gcgtcaacca gtgcgtcagc ctcagcatca ataagtgcct
      961 cagcttcagc aagcacatca gcttctgaat ctgcatcaac aagtgcgtca gcctcagcaa
     1021 gtacaagcgc ctcagcctca gcatcgacaa gtgcctcagc ttcagcatca acaagtgctt
     1081 cagcttcagc atcaacaagt gcgtcagctt ccgcatcaac gagtgcgtcg gcttccgcat
     1141 caacgagtgc ttcggcttca gcctcaacga gtgcctcagc ttcagcctca acgagtactt
     1201 cagtctcagc gtcaaccagt gcctctgaat ccgcatcaac gagtgcttca gcctcagcaa
     1261 gtaccagcgc ctcagcttca gcgtcaacca gtgcttcagc ctcagcatcg acaagcgctt
     1321 cagcttcagc gtcgacaagt gcttcagcct cagcaagtac cagtgcgtca gcttccgcat
     1381 caacgagtgc gtcggcttca gcaagtacta gcgcctcagc ctcagcctca accagtgcat
     1441 cagcctcagc aagtactagt gcctcagctt cagcatcaac gagtgcgtcc gcttcagcaa
     1501 gtactagcgc ctcagcctca gcgtcaacaa gtgcttcggc ttcagcgtca acgagtgcgt
     1561 ctgagtcagc atcaacgagt gcgtcagcct cagcaagcac atcagcatct gaatccgcat
     1621 caacaagtgc ctcggcttca gcatcaacga gtgcttcagt ctcagcgtca accagtgcct
     1681 ctgaatccgc atcaacaagt gcctcggctt cagcaagcac cagcgcctca gcctccgcat
     1741 caacaagtgc ctcatcctca gcaagcacat cagcatctga atccgcatca accagtgcgt
     1801 cagcctcagc aagcaccagt gcgtcggctt cagcaagtac tagcgcctca tcctcagcct
     1861 caacgagtgc atcagcctca gcaagtacta gtgcctcagc ttcagcatca acgagtgcat
     1921 cagcctcagc aagtatctca gcgtctgaat cggcatcaac gagtgcgtca gcctcagcaa
     1981 gtactagtgc ctcagcttca gcatcaacaa gtgcttcagc ctcagcatcg acaagcgcct
     2041 cagcttcagc gtcaaccagt gcctcagcct cagcatcaat aagtgcctcg gcttcagcaa
     2101 gtaccagtgc gtcagcttcc gcatcaacaa gtgcttcggc ttcagcgtca acgagtgcgt
     2161 ctgagtcagc atcaacgagt gcgtcagcct cagcaagcac atcagcttct gaatctgcat
     2221 caaccagtg
//
LOCUS       DAVKLX010000096         2139 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKLX010000096 DAVKLX010000000
VERSION     DAVKLX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.096"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..183
                     /locus_tag="VW325_002072"
     CDS             <1..183
                     /locus_tag="VW325_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9735383.1"
                     /translation="TSIIRQALKNTEYKDQISSTVLPDIKIKLPIDSRGTPDWNYMER
                     YIEDLKLKCNIANYNI"
     gene            239..556
                     /locus_tag="VW325_002073"
     CDS             239..556
                     /locus_tag="VW325_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HEU9735384.1"
                     /translation="MMASKRLSIEEQIEKKEESIKQLQNQKRQLKKKLNEQERKARNK
                     RLIEKGAVFESIFEESIDLTKDEFYKLIKTLNDEEIRLNIMEILEERIDDNVEKSSKD
                     EIT"
     gene            871..>2139
                     /locus_tag="VW325_002074"
     CDS             871..>2139
                     /locus_tag="VW325_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEU9735385.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIYDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEKWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNQLVKTIKEEIKTLK
                     GWIGNLLDNLSTAYEKFKDIERDKVIDNPKLFNLTNYLLTYSEIQKEKSKYLKGYAKT
                     NKEKYDFKKLTSAYSYLRKNNIETIGQLQTKIETLKSNSYRLNKKAKTIHKEMEDVEK
                     KILYYEIYKAKKEVYEEYQKKNIFTKEAFYN"
BASE COUNT         1028 a          254 c          348 g          509 t
ORIGIN      
        1 acttcaatta ttaggcaagc tttaaaaaat actgaatata aagatcaaat atctagcaca
       61 gtattgccag atatcaaaat taaattaccg atagacagta gaggtactcc agactggaat
      121 tacatggaaa gatatataga agacttaaaa ttaaaatgca atatagcaaa ttataatatt
      181 taaaacaaga acgattagaa ataaaaatct agtcgttttt tattttaaga aaggatacat
      241 gatggcaagt aaaagattaa gtattgaaga acagattgaa aagaaagaag aaagcattaa
      301 gcaattacaa aatcaaaaaa gacagttgaa gaaaaaactt aatgaacaag aaagaaaagc
      361 aagaaacaaa agacttatag aaaaaggtgc agtgtttgaa agtatttttg aagaaagtat
      421 tgatttaaca aaagatgaat tttataagtt aataaaaacg ttaaatgatg aagaaataag
      481 attaaacata atggaaattt tagaagaaag aatagatgat aatgtagaaa aatcatctaa
      541 agatgaaata acataattgt cccttggtta caagggcgca attatacacc ctaaagggtg
      601 cttgcgtcct gcggagtcaa acccttgcag ggagcaataa ccattcgctt tttaaaagtc
      661 aagggtaaat aaactcgcta aagctcgccc ttgacttttt aaaatttgaa atgaaagtaa
      721 caattaagcc gacatactga aacaacaaaa attaattcag tagtcggctt ttttaatttt
      781 atcatttcaa aacgctcatt cacaaagagg atataaaaaa actattaagc ctccacctaa
      841 aacaacaaag aaagaaagga agtgataaaa atggcagaca gttttcattt ttcagtaaac
      901 ataatatcaa gaggaaaagg caaaagtgca gtagcaagtg cagcatatat aagtggagaa
      961 aaaataaaaa atgaatggga cggagtaacc catgactaca caagaaaaga aaaaatatta
     1021 gtaaaaaata taatattgcc tgatcatata ccaaaagaat ttaatgatag gtctacctta
     1081 tggaataaag tagaaatggc agaaaaaaat tctaatgcac aactagcaag acaattcata
     1141 ataggactac caaaagagtt atctttaagt gaaaataaaa acctggttga aagatatata
     1201 aaagaaaatt taacatcaca aggaatgata gtagattatg caatttatga tgagagtcaa
     1261 gataaaaatg gaaatatcca ttgtcatata atgaccataa tgcgaccaat aaacgaaaaa
     1321 ggagagttct tagccaagtc aaaaaaagaa tatatcctag atgaaaaagg agaaaaagta
     1381 ttaaacaaaa atggaaaacc aaagacaaga aaagtagaac taacaacctg gaacgataca
     1441 ggcaatgtag aaaaatggag agaaaatttt tcagatcttt gtaacaaata tttagaaaga
     1501 gcaggagcag aaaaaagagt agaccatagg agttttaaaa gacaaaattc agattatcta
     1561 ccgacaatcc atttaggatc agcagcaagt gcaatggaaa gaaagggaat agaaactgac
     1621 aagggaaact ataatagaga aataagaaaa tataatcaac ttgtaaaaac aataaaagaa
     1681 gagataaaaa cattaaaggg ttggatagga aatttattag ataacttatc tactgcttat
     1741 gaaaaattta aggatatcga aagagataag gtaatagaca accctaaact ttttaatcta
     1801 acaaattatc tattaactta ttcagaaatt caaaaagaaa aaagtaaata tctaaaagga
     1861 tatgcaaaaa ctaataaaga aaaatatgac ttcaaaaagc taacaagtgc atatagttat
     1921 ttaagaaaaa acaacataga aaccataggt cagttacaaa caaaaataga aactttaaag
     1981 tctaatagtt ataggctaaa taaaaaagca aagacaatcc ataaagaaat ggaagatgta
     2041 gaaaagaaaa ttctatacta tgaaatctac aaagccaaaa aagaagttta tgaagaatat
     2101 caaaagaaaa atatattcac aaaagaagca ttttataac
//
LOCUS       DAVKLX010000097         2038 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKLX010000097 DAVKLX010000000
VERSION     DAVKLX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.097"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            7..1599
                     /locus_tag="VW325_002075"
     CDS             7..1599
                     /locus_tag="VW325_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9735386.1"
                     /translation="MKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMNLERFLKLS
                     IYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLS
                     WLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKI
                     FASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQ
                     RKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGE
                     LGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNICYKYGDKEI
                     LKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIKKTSYLNMV
                     SNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYVGDD
                     GRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIVT
                     HAPHPELKQYFTKIYQFPKDFI"
BASE COUNT          720 a          241 c          373 g          704 t
ORIGIN      
        1 tcaaatataa aaaaatttag tttattaacg atatttattg ttgctggtca attattgcta
       61 atttatgcag caactattaa tgctctggtg ttgaatgaat taattgcgat gaatttagag
      121 cggtttttga aattgtcaat ctaccaaatg attgtctggt gtgggataat attccttgac
      181 tgggtagtga aaaattatca ggttgaagtg atccaagagt ttaatctaga gattcgaaat
      241 agagttgcca cagacatctc taactctacc tatcaagaat ttcatagtaa atcatcagga
      301 acatatcttt cgtggctaaa taatgatgtt cagactttaa atgatcaggc ttttaaacaa
      361 ctttttttag taataaaagg aatttctggt actatatttg cagttgtgac tcttaatcac
      421 tatcattggt cattgactgt agccaccttg ttttcattaa tgattatgct acttgtacca
      481 aaaatctttg catcgaaaat gcgagaagtt agtctaaatt taactaacca aaatgaagct
      541 tttttaaaat ctagtgagac tatattgaat ggatttgatg tgttagcatc cttgaatctt
      601 ttatatgtat tgcctaagaa aattaaagaa gcaggaattt tattaaagat ggttatacaa
      661 agaaagacaa ctgtagaaac gttagcaggc gctattagct tctttctcaa tatttttttt
      721 cagatatctc tcgttttttt aacaggctat cttgcaataa aaggaatagt gaaaattggt
      781 actattgaag caataggagc actaacaggt gttattttta cagcgctagg tgaattagga
      841 ggtcaattat cctctattat tggtacgaag cctatttttt taaaattgta ttcaattaat
      901 ccaattgagt caaataaaat gaatgatatc gaaccaaatg aggtgaatag agattttccg
      961 ttatatgaag caaaaaatat ttgctataag tatggagata aagaaatatt aaaaaactta
     1021 aatttttgtt ttcaacgtaa tgaaaagtat ttaattttag gtgaaagtgg aagcgggaaa
     1081 tctacattat taaaattatt gaatggcttt ttgagagatt atagtggaga attgcgattc
     1141 tgcggggatg atataaaaaa aacctcctat ttaaatatgg tttcgaatgt tctatatgta
     1201 gatcaaaaag cttatttgtt tgaaggtacg attagagata atattttatt ggaagaaaat
     1261 tatactgatg aagaaatact acagtcttta gagcaagttg gtttgagtgt aaaagatttt
     1321 cctaataaca ttttagatta ttatgttggt gatgatggga gattactgtc aggagggcag
     1381 aaacaaaaaa ttactttagc tagagggcta attagaaata agaaaatagt attaattgac
     1441 gagggaactt ctgctatcga taggagaact tcgttagcga ttgaacgtaa gatattagat
     1501 agagaggatt tgactgtcat tattgttacc catgctccgc atccggaact taaacaatat
     1561 tttactaaga tatatcaatt tccaaaggat tttatttaag aatatagggg agggaaacat
     1621 gagattctat tgtgaagctt atgaggtaat tactgaagag aaaaaagtat actttaatga
     1681 tattgagtta gaagttaagt attcctctgt cgaagaattg tttataatat gtgagaaatt
     1741 attagataag aaaaaagtat cattttttta tgtagatgag aaacccttaa ggtatctgtt
     1801 atttgactat atatttctac tagttctagc taaaaaaata tcccaatctt atgaactgca
     1861 ccccaaaagt tagatttatt ctgtctaact tttggggtgc agttcatttt gcatagtttt
     1921 ggcattcatc ctagatatcc atataaaatg tctgagattg ttgaaatgtc taaaaaaata
     1981 tcagatctaa acgataaaaa agaatttcac aaaaagaact gtgagttgag gtttcaag
//
LOCUS       DAVKLX010000098         2033 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKLX010000098 DAVKLX010000000
VERSION     DAVKLX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.098"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..242
                     /locus_tag="VW325_002076"
     CDS             <1..242
                     /locus_tag="VW325_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9735387.1"
                     /translation="KFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQNE
                     FADFVVQVDKSKLAIQKSLEELETLKKSLMQEYFG"
     gene            302..538
                     /locus_tag="VW325_002077"
     CDS             302..538
                     /locus_tag="VW325_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU9735388.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            535..948
                     /locus_tag="VW325_002078"
     CDS             535..948
                     /locus_tag="VW325_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9735389.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            1066..2031
                     /locus_tag="VW325_002079"
     CDS             1066..2031
                     /locus_tag="VW325_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9735390.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
BASE COUNT          740 a          315 c          388 g          590 t
ORIGIN      
        1 agaaatttat tatccatgtt ttaaggaata ataattatag tcgagtgata tcaggaagtg
       61 ctcagcctca gttaccaatt acaaaattaa aaaaaatact tctccccctc cccccactag
      121 ccctccaaaa tgagttcgca gactttgtag tccaggtcga caaatctaaa ttggcaatcc
      181 aaaaatctct ggaagaactt gaaactttga agaaatctct gatgcaggag tattttggct
      241 gatattctgc cattgtaatt acggtaatga tttgttataa tacttcaaag gaggaaatca
      301 gatggtagta aaaacaagaa aacaaggaaa ttcaatcacc attacgattc caagtgaatt
      361 taatattcca agtggtgtta aatacgaagc gaaattgtta ccaagtggtg agattatctt
      421 tactcctgaa gaattggggc agcaggtttc ttatgtatct gatgatgcct ttgacttaaa
      481 tttagataaa atatttgacg aatacgacga tgttttcaaa gctttggtgg aaaaatgaca
      541 atctatctga cagaaaagca aattgaaaaa ataaatgctt tagcaattca acggtattct
      601 ccaaatgaga aaattcaaac agttagttct tctgccttaa atatgattgt gaacttacca
      661 gaacaatttg tctttgggaa gtctctttat ccaacaattt ttgataaagc aacgatacta
      721 tttgtccaat tgataaagaa gcatgttttt gctaatgcta ataaaagaac tgctttcttc
      781 gttttggtca aatttttaca attaaacggc tatcgttttt ctgtaacggt agaagaagca
      841 gtaaaaatgt gtgtaaccat cgcagtagaa gctttaactg atgaaaaaat gacaagctac
      901 tccaaatggg tttctgaaca ttctgttaga gaaaaggtca aaaagtaacc tagtatgctg
      961 gatttgaatg agcacaagaa aataaatgaa cagacaatat tagaattctg taatgcagaa
     1021 actgatattg tctcttttta ttgatgaata agaaagtgag aaattatgga atcaaaagtt
     1081 acaattatca tgcaagaaat gttacctctt ttaaataatg aacaattact agcgttgaga
     1141 gagagtttag aacatcatct agtagacgga aaaaagcagc agaagtattc gaataataac
     1201 ctgttgcaac tatttattac cgccaagcag gtagagggct gtagctcaaa aacaattcgt
     1261 tattaccaga ggacgattga aaacttgttt aatgctatta aagagtctgt gacacaactc
     1321 acaacagatg atttaaggag ttatttagca aattaccagt ctgaaaagga ttgtagtaag
     1381 gcaaatttag acaatattag gcgtatattg tcttcttttt ttgcttggct tgagcaagag
     1441 gaatatatca ttaaaaatcc cattcgacgg ataaagaaaa ttaagactga gcaaaatgtg
     1501 aaggaaactt atactgatga acatttggaa attatgcgtg ataactgtga aaatttgaga
     1561 gatttggcaa taatagacct actagcatcg acaggtatgc gtgtagggga gcttgtacag
     1621 ttgaatcgtt cagatattga ttttgaaaac agagagtgtg ttgtctttgg taaaggaaag
     1681 aaggagagac cagtatattt tgacgctcgg acgaaaattc atttaagaaa ttatcttaac
     1741 gacagaaaag atagtcaccc tgctcttttt gtaacgctag ttggaaaagc ccagagactt
     1801 ggaattgctg gtgtagagat tcgcttaaga aagttaggag acaaactcgg catacaaaag
     1861 gttcacccac ataagttcag aagaacttta gcgactaagg caattgataa aggtatgcct
     1921 atcgaacaag tccaaaaact gctaggtcat agcaagattg acacaaccct ggcctatgcc
     1981 atggtcaatc aaaataatgt caagcattca caccaaaaat tcatctctta aaa
//
LOCUS       DAVKLX010000099         1873 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKLX010000099 DAVKLX010000000
VERSION     DAVKLX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.099"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..1712)
                     /locus_tag="VW325_002080"
     CDS             complement(<1..1712)
                     /locus_tag="VW325_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEU9735391.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNGTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYVASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGKGVDSVYNVPIYYRLTVTNDGLKLTFTYTVTYVDPKTKTLGNISKKMSN
                     GYSIYNTGTSIQTMLTLGSGLGTPSDVKNSITNKNGGQVQYYNTSTMTKRGSRYTWGN
                     GAQMNGWQAKREYGLTSSWTVPITGTDTSFTFTPYAAKTDNIRTNYFNGRGKVVESST
                     TSQSLSQSKSLSVSASQSASASASTSASASASTSASASASTSASASTSASASASTSAS
                     ASASTSASESASTSASASASTSASASASTSASASASTSASASASTSASASASTSASVS
                     ASTSASASASTSASASASTSASESASTSASESASTSASASASTSASASASTSASASAS
                     TSAS"
     gene            complement(1710..1823)
                     /locus_tag="VW325_002081"
                     /pseudo
     CDS             complement(1710..1823)
                     /locus_tag="VW325_002081"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
BASE COUNT          470 a          404 c          423 g          576 t
ORIGIN      
        1 tctgatgcac tggttgacgc tgaagccgaa gcactcgttg atgctgaagc tgatgcacta
       61 gtacttgctg aggctgacgc actcgttgat gcagattcag acgcactggt tgatgcagat
      121 tcagacgctg aggtacttgc tgaggctgac gcactcgttg aggctgaagc tgaagcactg
      181 gtgcttgctg agactgatgc gctggtactt gctgaagctg aggcactggt tgatgcggaa
      241 gctgaggcac ttgtcgatgc tgaagccgaa gcactcgttg acgctgaagc cgacgcactg
      301 gtgcttgctg aagctgatgc actcgttgat gcagattcag acgctgatgt gcttgctgag
      361 gctgaagcac tggttgacgc tgaagccgac gcactggttg aagccgacgc actggtgctt
      421 gctgaagctg aggcgcttgt gcttgctgag gctgaggcac tggttgatgc tgaggctgag
      481 gcgctttgac tagcacttac tgagagtgac ttagactgtg aaagtgactg actggtcgtg
      541 ctagattcaa ctacctttcc tctaccattg aagtagttag ttctaatgtt atctgtttta
      601 gcagcgtaag gggtaaatgt aaaggatgta tccgttccag taattggtac agtccaagat
      661 gatgttaatc catattctct cttagcctgc caaccattca tttgggcacc atttccccaa
      721 gtatacctac taccccgctt cgtcattgta gatgtattat agtattgaac ctgtccacca
      781 tttttgttag taatggagtt ctttacatct gaaggggtac caagaccact gccaagggtt
      841 aacattgttt gtattgaagt tcctgtatta tagatagaat atccattact catctttttt
      901 gatatattac caagagtctt tgttttagga tccacatacg taaccgtata ggtaaaggtc
      961 agttttaagc catcatttgt cactgtcaat cgatagtaaa ttggaacatt atataccgaa
     1021 tctacacctt tacctgtata aacttttgtc tcacttttag caatgtttag acttgcatta
     1081 attgcagggg cgccatttac aatcgtattg ccagaaaaaa cagcagcttt tatagaagcc
     1141 agcaattgct cgatggaatc cactgaacgc ttgcgtcgct tagcataaga ttggagattg
     1201 acatttgtaa ctgatgctac ataatcacta gctggtttct tacgatcttc ttcgaccttc
     1261 ttagcagttg cttctgtagc ggcagctgtt tgtgatccta ccacagtaga tgatgcagag
     1321 atacttgtcg aagccgatgt acttgctgat tctgatgcac ttgtactcgc tgaggtcgaa
     1381 gcgctggttg aggctgactc agatgcagat gtacttgccg actctgaagc tgacaaacta
     1441 gttgaactcg ctgaattact tgtagatatc gtacctagaa ctactgtacc atttgttgcc
     1501 aaagcatccg ttttctctac agttttttca agtactgccg actcatttgt aaataccttc
     1561 gtttgagttg caacggttcc acttatgaca gttcccgcag caactatccc cttgaggata
     1621 tcaagcccag taattgaatg acttacttta tcttctaccg tttcggtcat gacctgagta
     1681 gtatcaacac caccacgcat gaccttaaac aagccaaaaa gagaagtcga ggcccgtagc
     1741 caatgcttcc ctgatttgat taatttgaac cgagttactc ggtctgtttc tctatattca
     1801 cccttctgac gtctaaaaaa tattttgaaa tttcctttcg agtattattt tcatgtatgg
     1861 atataaataa att
//
LOCUS       DAVKLX010000100         1791 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKLX010000100 DAVKLX010000000
VERSION     DAVKLX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.100"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            365..841
                     /locus_tag="VW325_002082"
     CDS             365..841
                     /locus_tag="VW325_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9735392.1"
                     /translation="MIGKNIKSLRKTHDLTQDDFSRIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            841..1602
                     /locus_tag="VW325_002083"
     CDS             841..1602
                     /locus_tag="VW325_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU9735393.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT          656 a          241 c          349 g          545 t
ORIGIN      
        1 agtaaaaaca acactttatt gaggtattgg atacgctttg ctaatagcct aataatcaca
       61 tgtggagtgt tgctacaacg aaaaaggtga taatccttaa tttcaagcta ttttataagc
      121 attttgtctt tgtagataaa ggcaattttg acaataaaaa tcctaaaagg tgaatcgtta
      181 tagatgtatt tgtagatatt gtttgcgcat cgaaaaaatt aatacaagaa taaatattta
      241 tagctcttta ggtgactttt atagaagtaa agtttaggat agaaaaacaa gaaataacgc
      301 accatttttg gtgcgttatg cttttttatg ctataatgga tttataaaaa taaaggagtt
      361 tgctatgatt ggaaagaaca taaaatcctt acgtaaaaca catgacttaa cacaagacga
      421 cttttcccga attgtaggaa tttctcgaaa tagcttgagc cggtatgaaa atggaactag
      481 ttcagtctct acggaactaa tagacatcat ttgtcagaag tttaatgtat cttatgtcga
      541 tattgtagga gaagataaaa tgctcaatcc tgttgaagat tatgaattga ctttgaaaat
      601 tgaaattgtg aaagaaagag gtgctaatct attatctcga ctctatcgtt atcaagatag
      661 tcagggaatt agcattgatg atgaatctaa tccttggatt ttaatgagtg atgatctatc
      721 tgacttgatt catacgaata tctatttagt agaaactttt gatgaaatag agagatatag
      781 tggctatttg gatggaattg aacgtatgtt agagatatct gaaaagcgga tggtagctta
      841 atggaaatcc aagattatac tgatagtgaa ttcaaacatg ctttagcaag gaatcttcgt
      901 tcactgacaa gaggaaaaaa gtccagtaag caacctatag cgattttgct tggagggcaa
      961 agtggtgccg gtaagactac aattcatcgt attaaacaga aagaatttca aggaaatatt
     1021 gttatcatag atggtgatag ttttcgttct cagcatccac actatttaga actgcagcaa
     1081 gaatatggca aagacagtgt agaatatacc aaagattttg caggaaaaat ggtagagtct
     1141 ttagtaacaa aattgagtag tttgagatac aatcttttga tcgagggaac tttacgaaca
     1201 gttgatgttc caaagaaaac agcacaactc ttgaaaaata agggatatga agtacaattg
     1261 gccttaattg cgacaaagcc tgaattgtcg tatctaagta ctcttatccg ttatgaagaa
     1321 ctgtacatta tcaatccaaa tcaagcacgc gcaactccaa aagaacatca tgatttcatt
     1381 gtaaatcatc tagttgataa tacacgacaa ttggaagaac tggctatctt tgaaagcatt
     1441 caaatttacc aacgagatag aagttgtgta tatgattcaa aagaaaatac aacttcagca
     1501 gcagatgttc ttcaagagtt actctttggg gagtggagtc aggtagagaa ggagatgttg
     1561 caggtggggg aaaagagact taatgaatta cttgaaaaat aaacaattga tatttttagg
     1621 agaatagaaa tgagagggtt taataacaag ataaagtctg tttatcaaga actaacaaat
     1681 tccaaagaga aattcggtag ctttcacaag actttaattc atttgcatac acctgtttct
     1741 tatgattaca agctattttc taattggact gcaacgaaat atagaaaaat t
//
LOCUS       DAVKLX010000101         1664 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKLX010000101 DAVKLX010000000
VERSION     DAVKLX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.101"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            84..491
                     /locus_tag="VW325_002084"
     CDS             84..491
                     /locus_tag="VW325_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9735394.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATPFKEVQKPLENFN
                     HIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            656..955
                     /locus_tag="VW325_002085"
     CDS             656..955
                     /locus_tag="VW325_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9735395.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1216..1539
                     /gene="tnpB"
                     /locus_tag="VW325_002086"
     CDS             1216..1539
                     /gene="tnpB"
                     /locus_tag="VW325_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9735396.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          565 a          328 c          292 g          479 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact cctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tcaaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattgacta atactcttcg aaaataaaat tcaaaccaca tcagcgtcgc cttaccatac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttt attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcagctag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag gcctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataata aaacgcatag
     1021 tagcaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcggag
     1201 gaaaagtatt gtactatgat gattcaacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgataca aggaatcgat tcactggctt atctcgttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     1381 aaagcccttt actgggatgg tcaaggattt tggatactat ataaacgctt tgagaacgga
     1441 aaactgactt ggcccagtac agaaaaggat gtcaaagctc tcgcacctga acaagtagac
     1501 tggcttatga agggcttttc tatcactccc caaatatagt ggatttaaac tagaatagta
     1561 cacccctact tctaaaacat tgttagaaat cgatttgact gtcctgatca atttgtcctg
     1621 ttcttgtttc attttactat agaacgattt gaaggcgttt atga
//
LOCUS       DAVKLX010000102         1541 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKLX010000102 DAVKLX010000000
VERSION     DAVKLX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.102"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            124..>1541
                     /gene="pcpA"
                     /locus_tag="VW325_002087"
     CDS             124..>1541
                     /gene="pcpA"
                     /locus_tag="VW325_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU9735397.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSVI
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLW"
BASE COUNT          604 a          208 c          247 g          482 t
ORIGIN      
        1 gacaatttat aaaatgtact actctattct actagataat gattgaataa aactttttat
       61 aacaacggct agcaaagtta taattttata tctattagaa aataaatatg taaggaaatt
      121 taaatgaaaa aaactacaat attatcatta actacagctg cggttatttt agcagcatat
      181 gtccctaatg aaccaatcct agcagatact cctagttcgg aagtaatcaa agagactaaa
      241 gttggaagtg ttattcaaca aaataatatc aaatataagg ttctaactgt agaaggtaac
      301 ataggaactg ttcaagtggg taatggagtt actcctgtag agtttgaagc tggtcaagat
      361 ggaaaaccat tcacgattcc tacaaaaatc acagtaggtg ataaagtatt taccgttact
      421 gaagtagcta gtcaagcttt tagttattat ccagatgaaa caggtagaat tgtctactat
      481 cctagctcta ttactatccc atcaagcata aaaaaaatac aaaaaaaagg cttccatgga
      541 agtaaagcta aaactattat ttttgacaaa ggcagtcagc tggagaaaat tgaagataga
      601 gcttttgatt tttctgaatt agaagagatt gaattgcctg catctctaga atatattgga
      661 acaagtgcat tttcttttag tcaaaaattg aaaaagctaa ccttttcctc aagttcaaaa
      721 ttagaattaa tatcacatga ggcttttgct aatttatcaa atttagagaa actaacatta
      781 ccaaaatcgg ttaaaacatt aggaagtaat ctatttagac tcactactag cttaaaacat
      841 gttgatgttg aagaaggaaa tgaatcgttt gcctcagttg atggtgtttt gttttcaaaa
      901 gataaaactc aattaattta ttatccaagt caaaaaaatg acgaaagtta taaaacgcct
      961 aaggagacaa aagaacttgc atcatattcg tttaataaaa attcttactt gaaaaaactc
     1021 gaattgaatg aaggtttaga aaaaatcggt acttttgcat ttgcggatgc gattaaactt
     1081 gaagaaatta gcttaccaaa tagtttagaa actattgaac gtttagcctt ttacggtaat
     1141 ttagaattaa aagaacttat attaccagat aatgttaaaa attttggtaa acacgttatg
     1201 aacggtttac caaaattaaa aagtttaaca attggtaata atatcaactc attgccgtcc
     1261 ttcttcctaa gtggcgtctt agattcatta aaggaaattc atattaagaa taaaagtaca
     1321 gagttttctg tgaaaaaaga tacatttgca attcctgaaa ctgttaagtt ctatgtaaca
     1381 tcagaacata taaaagatgt tcttaaatca aatttatcta ctagtaatga tatcattgtt
     1441 gaaaaagtag ataatataaa acaagaaact gatgtagcta aacctaaaaa gaattctaat
     1501 cagggagtag ttggttgggt taaagacaaa ggtttatggt a
//
LOCUS       DAVKLX010000103         1473 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKLX010000103 DAVKLX010000000
VERSION     DAVKLX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.103"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            263..>1473
                     /locus_tag="VW325_002088"
     CDS             263..>1473
                     /locus_tag="VW325_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735398.1"
                     /translation="MLAEAEALVDADSDAEVLAETDALVLAEAEAEALVDAEADALVD
                     ADSDALVDAEVEALVLAEAEALVLAEADALVEAEAEALVEAEAEALVDADSDAEVLVD
                     AEAEADVDADSDALVLAEAEALVEAEAEALVLAEADALVDAEADALVDAEAEALVDAE
                     ADALVEADSDAEVLAEAEALVEADSDAEVLAEAEALVDAEAEALVEAKAEALVDAEAD
                     ALVDAEADALVDAEAEALVDAEADALVLAEADALVDADSEAEVLAEADALVLAEADAL
                     VLAEADALVDADSDADVLAEADALVDADSDAEVLAEADALVDADSEALVDADVLALVD
                     AEADALVLAEAEALVDAEAEALVDAEAEALVLAEAEALVDAEAEALVEADSDAEVLAE
                     ADALVLAEAEAL"
BASE COUNT          290 a          332 c          484 g          367 t
ORIGIN      
        1 tgaggctgaa gctgacgcac tcgttgatgc tgactcagac gcacttgtac ttgctgaagc
       61 cgacgcactc gttgatgctg agactgatgc gctggtactt gctgaggctg acgcactggt
      121 tgacgctgat tcagatgctg atgtgcttgc tgaggctgag gcactcgttg atgctgaagc
      181 tgaggcgctt gtcgacgcgg aagctgacgc actcgttgac gctaaagccg acgcacttgt
      241 tgatgccgat tcagacgctg aggtgcttgc tgaagctgag gcactcgttg atgccgattc
      301 agacgctgag gtacttgctg agactgatgc gcttgtgctt gctgaggctg aagctgaggc
      361 acttgttgat gctgaggctg acgcactcgt tgatgcagat tcagacgcac tggttgatgc
      421 tgaagttgag gcactagtac ttgctgaggc tgaggcactg gtacttgctg aagccgacgc
      481 actcgttgag gctgaagctg aggcactcgt tgaggctgaa gctgaggcac tggttgatgc
      541 tgattcagac gctgaggtac ttgttgacgc tgaggctgag gcagacgttg atgctgactc
      601 agacgcactt gtacttgctg aagctgaggc actcgttgag gctgaagctg aggcgcttgt
      661 acttgctgaa gccgacgcac tggttgacgc tgaagccgac gcacttgtcg atgctgaggc
      721 cgaagcactg gttgacgctg aagccgacgc acttgttgag gccgattcag acgctgaggt
      781 gcttgctgaa gctgaggcac ttgttgaggc cgattcagac gctgaggtac ttgctgaagc
      841 tgaggcgctt gttgatgctg aagctgaagc actcgttgag gctaaggctg aggcactcgt
      901 tgatgctgaa gctgatgcgc ttgtcgatgc tgaagccgat gcactcgttg atgctgaagc
      961 tgaggcgctt gtcgatgctg aagccgacgc actagtactt gctgaagccg acgcgctggt
     1021 tgatgccgat tcagaagctg aggtgcttgc tgaagctgat gcactggtac ttgctgaagc
     1081 tgacgcactg gtacttgctg aagctgacgc actggttgac gctgattcag atgctgatgt
     1141 acttgctgag gctgacgcac tcgttgacgc tgattcagat gctgaggtac ttgctgaagc
     1201 cgatgcactt gttgatgcgg attcagaggc gctcgttgat gctgacgtac ttgcgctggt
     1261 tgatgctgaa gccgacgcac tggtgcttgc tgaagctgaa gcgcttgtcg atgctgaggc
     1321 tgaagcactt gttgatgcgg aagccgaagc actggtgctt gctgaagctg aagcgcttgt
     1381 cgatgctgag gctgaagcac tggttgaggc cgattcagac gctgaggtac ttgctgaagc
     1441 tgatgcactg gtacttgctg aggctgaggc act
//
LOCUS       DAVKLX010000104         1455 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKLX010000104 DAVKLX010000000
VERSION     DAVKLX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.104"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            142..>1455
                     /locus_tag="VW325_002089"
     CDS             142..>1455
                     /locus_tag="VW325_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEU9735399.1"
                     /translation="MNIPEERTDSPLRIAQYYYDTFGKNQYTKKIEEIFFKKGLSKPN
                     ELHKLIEKIAPKHIITTNYDSLLESQFESGLLKYNVVAEDKDIPYTSSERYLIKMHGD
                     FSKKNIVLKEDDYLDYHLNFPMISTLIQSLIMNHTLLFVGYSLSDSTFNSIFRMIQNT
                     FKLDAKNAYFYTPEEPSMIIREYYKKQGIFIISNEENLGQETSEKQNKLYCRTKDFLE
                     VLSENRSQDVNNADDLWNQLAFLDRLSFIDAKDFSRYSDLKKRALNWDDEYCWFGNDQ
                     LRFEIDGHEELRIMVSKKSLLNRFLDMEIGEPRDLKGNRFLSKAFKLYEEKQYSLAKA
                     KFRELANIAFRQKDYFNFLVCEFNFQQIQIIDRYEKTPSYAEPLYDGELSELTEQILN
                     SVTGDEKKIIEFFRDSILNFNFLYRKLETINELFDEIREEHESYRR"
BASE COUNT          555 a          168 c          258 g          474 t
ORIGIN      
        1 gaagaaaatt ataatttatc tataactcaa ataaagaatt ctattaaaga aaattcctta
       61 gttttgtttg ttggtgcagg aatatcagct aattctaatc taccaacttg gggagaattg
      121 attcaatcat taaaaaaaga attgaacata ccagaagaaa gaacagatag tccactgaga
      181 attgctcagt attattatga tacttttggg aaaaatcagt acacaaagaa gatagaagaa
      241 attttcttta agaagggatt gagtaaacca aatgagttac ataaattaat agaaaaaatt
      301 gcaccaaaac atattattac gacaaactat gattcacttc tggagagtca atttgaaagt
      361 ggcttgttaa agtacaatgt agtcgctgag gataaagaca tcccttatac gagttcggaa
      421 agatacttga ttaaaatgca tggtgacttc agtaagaaaa atattgtttt gaaagaagat
      481 gattatttag actatcattt gaacttccca atgatttcta ctttaatcca gtcattgata
      541 atgaatcata ctctattgtt tgtggggtat tctctaagtg attcgacttt taattcaatt
      601 tttagaatga ttcaaaatac atttaaattg gatgctaaaa atgcttattt ttatactccc
      661 gaggaaccgt ctatgatcat tcgagagtat tataagaagc aaggaatttt cattatctct
      721 aatgaagaga atctaggtca ggagacatca gaaaaacaga acaaattgta ttgcaggaca
      781 aaagattttt tagaggttct atcagaaaac cgaagtcaag atgtaaataa tgctgatgat
      841 ttatggaatc aactggcttt tttagataga cttagtttta ttgatgcgaa agatttttct
      901 agatattctg atttaaagaa aagggcttta aactgggatg atgagtattg ctggtttgga
      961 aatgatcaat taagatttga aattgatggt catgaagaat tacgaattat ggtatctaaa
     1021 aaatctttat taaatcgctt tctagatatg gagattggtg agcctaggga tttaaagggg
     1081 aatcgattct taagtaaggc ttttaagttg tatgaagaaa aacaatattc tttagcaaaa
     1141 gcaaaattta gagaactagc taatatagca tttcgtcaga aagactattt taatttctta
     1201 gtttgtgaat ttaattttca gcaaattcaa ataattgata gatacgaaaa aacacctagt
     1261 tatgcagagc cactttatga tggagaatta agtgaattaa cagaacaaat tcttaactcg
     1321 gttacaggag atgagaaaaa gattatagaa ttttttagag attcaatcct taacttcaat
     1381 tttttatata gaaaattaga aactattaat gaattatttg atgaaattcg tgaagagcat
     1441 gaaagctata gaaga
//
LOCUS       DAVKLX010000105         1220 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKLX010000105 DAVKLX010000000
VERSION     DAVKLX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.105"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            144..317
                     /locus_tag="VW325_002090"
     CDS             144..317
                     /locus_tag="VW325_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735400.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
     gene            503..1030
                     /locus_tag="VW325_002091"
     CDS             503..1030
                     /locus_tag="VW325_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEU9735401.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKVKPNKKDMTREI"
     gene            1157..>1220
                     /locus_tag="VW325_002092"
     CDS             1157..>1220
                     /locus_tag="VW325_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEU9735402.1"
                     /translation="MATNKRYYWIKLKEEFFTDKR"
BASE COUNT          649 a          164 c          188 g          219 t
ORIGIN      
        1 agaccaagtt aataaagata atgaatacag agaaatgatg aaaaacaaga aaatggatca
       61 agaaagataa gacctggtaa aaatatctta tccaagctat aatatgacca aacaactaaa
      121 gtcagatcta ggaggtaata gagatgaata aaaggcaaga gctaatagat gaactcataa
      181 aagcaaatga agatggaaca tacaaaacct ataaaagcac agaagaaata aaagcaatga
      241 acaatgaaga aatacagatt atatattcca acatgaaaaa ctatctatca gacaaaagaa
      301 cacacataaa ctattaaagg tggaaaggta gtgtaagcta tttttaaaag tataaaaggt
      361 acaaagacct aagaaatata taaaaacatc aaaataaagt attccaccac caagaacaat
      421 aaaataaaaa caagcaaagg gaagtataga aaaattaaaa cctatacttc ccttttttaa
      481 ttcaaacaaa ggagagaaaa gcatgataaa cgaagaaata agcaaggaag caggtcaagc
      541 agcacaaacc ataatatcat acacaataaa ggcaacaaaa gaatcaatca atttagaaaa
      601 agaaataaga aaaaagatga atgaaacttt agaaaaagca aatggaaact taaaaagtct
      661 tatgggcgat gaaatgaaaa taaaagatct ctacaagaaa ggacaactag aaaatataag
      721 catagatcaa atcgacctca aagacttaaa aaaagaacta aacaaacttg gagtaagttt
      781 ctcagtaatg aaaaacaaag aaagcaaaaa ctatgaaata ttcttccaag ccaaagacat
      841 aaaagtaatg gaatatgcct ttaagcaagt catagccaag gaaaataaaa aagaaaaaga
      901 aagtatccta aaacaaataa agaaatacaa agacctatcc aaaaacaaag ataagacaaa
      961 agaaaaagga aaaaggaaag taaaagaaaa agtaaaacca aacaaaaaag atatgaccag
     1021 agaaatctaa agggagtaac gaaaagttac acccttaaat aaccataata acaagacttc
     1081 cgaaaaacag aagtccagaa ttccgaaaat cggaaatcaa gaatttcgat tatcgaaaat
     1141 caagaaagga gcagatatgg caacaaacaa aagatactat tggataaaat taaaagaaga
     1201 atttttcaca gacaaaagga
//
LOCUS       DAVKLX010000106         1174 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKLX010000106 DAVKLX010000000
VERSION     DAVKLX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.106"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            78..428
                     /locus_tag="VW325_002093"
     CDS             78..428
                     /locus_tag="VW325_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9735403.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWTYLCSQVN
                     QNLEKIRQREGDTH"
     gene            643..759
                     /locus_tag="VW325_002094"
                     /pseudo
     CDS             643..759
                     /locus_tag="VW325_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
     gene            752..985
                     /locus_tag="VW325_002095"
     CDS             752..985
                     /locus_tag="VW325_002095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735404.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEELL"
BASE COUNT          384 a          227 c          246 g          317 t
ORIGIN      
        1 tctataaatt aatttgactt tcctgataga gttattcaca tcttatttca actcactata
       61 gaaggaggaa taggaggatt ctcagacatc cgggcatcag cccaactaat gatttgattg
      121 ctaagaaaat attcagcaat ccagaaatca cttgtcaatt tattcgcgat atgctggact
      181 tgccagcaaa aaatgtgacc attttggagg gaagcgatat tcacgtatta ctctccatgc
      241 cttactcggt gcaggatttt tataccagta tagacgtctt ggcggagttg gataacggta
      301 ctcaagtaat tattgagatt caagtccatc atcagaattt tttcatcaat cacttgtgga
      361 cttacctgtg cagtcaggtt aatcaaaatc ttgaaaaaat tcgtcagcga gaaggtgata
      421 ctcactagag ctacaaacac atcgctcctg tttacgccat tgctatcgtg gatagtaatt
      481 atttctcaga tgacctggct tttcatagct ttagtatgcg cgaagacaca acaggtgagg
      541 tattggcgat taccaacaat ggacaggaaa accatctggt taagatggca ttcttggaat
      601 taaaaaatac agagaaacca gcaaagacaa ggttcgcaag ccatggttgg agtttttcgg
      661 caacaagccc tttacccagc aaccgcaacg agccattagc caagcaaatc aactgctgga
      721 ctacaagagc tggtccgagg aggacaggaa aatgtttagt caactacata tgcgagaaga
      781 acaagtcttg ttagcacagg actatgcctt ggaaactgct agggctgaag gccttgaaca
      841 aggactagag cgtgggaaag ttgaaggaag ggcagaaagg aaactttttg ccttcctaga
      901 catagtacgc caaggtcttc tgacttctga ggttgccagc cagcaattag gtatgtcagt
      961 atctgaattt gaggaactgt tgtaaaatgg ctccataata tccatagtgg gtaaatcccc
     1021 tatggatatt atggagccta ttttgtgtag aaaaaaagtc ccatatgacc tataatgaaa
     1081 agcgacaaaa caactcatta gaaagaatca tatggaacaa ttacatttta tcacaaaatt
     1141 actagacatt aaagacccta atgtccagat ttta
//
LOCUS       DAVKLX010000107         1071 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKLX010000107 DAVKLX010000000
VERSION     DAVKLX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.107"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            240..839
                     /locus_tag="VW325_002096"
     CDS             240..839
                     /locus_tag="VW325_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735405.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEGTGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            976..1071
                     /locus_tag="VW325_002097"
                     /pseudo
     CDS             976..1071
                     /locus_tag="VW325_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          385 a          161 c          211 g          314 t
ORIGIN      
        1 cgttaatatt tggagaagag gatggccatc ttctccattt ttatgtgcag agttatagtg
       61 acttgatgct gggaaagtac actgtgactg ctaatactca atgaaaatca aagtgcaaac
      121 taggaagcta gccgcaggct gtacttgagt acgtcaaggt gaagctgacg tggtttgaat
      181 ttgattttcg aagagtataa aacattccta gaagttactt tgatttccct aatctattta
      241 tgcaaatttt atgttatttt actataacag ttgttgctaa gccaaataat agtggggaag
      301 ttcatttaga cgtaagcatt gaagataatc agggaggtag tgggcataat ttcagttctg
      361 tttcaagtag ctcacaaaca gctaaatatg aaggaactgg ttataataac aattcatcat
      421 tatatataac gattgataaa acgtctgatg caacagctct tttgaaatta aagttgaata
      481 atgttgataa tcaacctgct actgaagttc ctagttcagg aattactgta aaattaaatg
      541 ctaaagataa tgctggaaac tggacaagtg cttcgaataa aaaagaagta acagtaaaaa
      601 ttgtttctgc taaaccgaca tatccagaca aaatcttagt gaaaaatcct gataatataa
      661 aagatacaga aaaaaatgcc attattgaaa aattgaaaga ggcaaataaa aatcatccag
      721 caggagctcc aacctttgct aaaggtgaag gagagcatgc aaatgatatt gtagcaactt
      781 attcagatgg tacaacttat tatgtaccgt taaatgatgt gacaaaatat gcgaggtagt
      841 ggctgtacca ctcacttatt cacctccccg tgatttgtag tagtgatagg ctttctcact
      901 attattataa aacaaaataa agatcacaac actttttcat tctgtgttgt gccttgagtg
      961 aaacgaaagg aatgaattat aaatatgaaa agtatagtca ctagcataga tgagcgcttg
     1021 cgtactcgcc tacgagtgat tatctggaag caatggaaga agaaatcgag a
//
LOCUS       DAVKLX010000108         1018 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKLX010000108 DAVKLX010000000
VERSION     DAVKLX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.108"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..160)
                     /locus_tag="VW325_002098"
     CDS             complement(<1..160)
                     /locus_tag="VW325_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEU9735406.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELMKF"
     gene            complement(173..922)
                     /locus_tag="VW325_002099"
     CDS             complement(173..922)
                     /locus_tag="VW325_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU9735407.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
BASE COUNT          352 a          197 c          114 g          355 t
ORIGIN      
        1 aaaacttcat aagctcttca tttttagatg gaagaatgcc cttttttaaa ttatttattt
       61 ttacagaatc tatatcatat gcaacaactt tatatttaga tgcaaatagt aacgcgtagg
      121 ccagcccaac atgccccaaa ccaattactg ctatattcat aaaactactt ccttatttct
      181 taatccaaaa tctaatagaa taagctgccc cattccttaa atacaactct ttaatattgt
      241 ttaaaagttt ttcaactgat ttccagatta tcaaaatctg agatttatag cacaatattg
      301 atgatattct atcaatataa tgtttttcat caagttcctc ttgatacatt tttaattctt
      361 tagtttttcc catataacta accatactac tatcacttac atatgggaag tcctcataat
      421 atattacttt ataacgcata aattcaagcg cccttccaat actattcaca aaaacatgat
      481 caacatggtc accaagtgaa agcggacaat atacgacaca tttgtcgtct aaatgcatta
      541 acagctcttt tatgatatca ttctttaatg tgtcctcatt ttttaattca ctatagatat
      601 gacggtatag aaaattgcca tttctatctt tcctatagag acattcatag tacgataagt
      661 gtctaaaatc acactgtaga cgttcacaag ctaacctgtc ttctttcttc ctttcttcaa
      721 tcggatattt cccaaggtta cacaacttat gaaattgctt agcagagggc tgtagctgtt
      781 ggctcaaagg gtaaccagaa aatatagtaa taacaagtac aatttctcct tctgaagtta
      841 attttgaaat ataatcacca caggaaaaaa ttgcgtcatc taaatgtgga gataaaaaga
      901 tatacttagt attgttactc ataaccattc cctctacaat ttatctaaaa actcactaag
      961 tgtctgatta aattccacat catcaaaaaa attcacctta ttcttaataa tgaatatt
//
LOCUS       DAVKLX010000109          952 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKLX010000109 DAVKLX010000000
VERSION     DAVKLX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.109"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            105..584
                     /locus_tag="VW325_002100"
     CDS             105..584
                     /locus_tag="VW325_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9735408.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            678..749
                     /locus_tag="VW325_002101"
     tRNA            678..749
                     /locus_tag="VW325_002101"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:711..713,aa:Glu,seq:ttc)
BASE COUNT          353 a          132 c          223 g          244 t
ORIGIN      
        1 gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtgtataagt gtagaaatga atattacctt catttatggg
      181 aattgtcgga ttgggaccaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggt agacgatatt ccacgtttaa ggaaatattt caaaaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aaacaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggtgagatc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgcgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ttagtaagtc atgcaaaaaa
      601 aatgaaaaaa attagaaaaa gtagttgaca aagtttgaaa agactgtata atagtaagag
      661 ttgaaaataa caactcaggt ccgttggtca aggggttaag acaccgcctt ttcacggcgg
      721 taacacgggt tcgaatcccg tacggactat ggtatgttgc gtcaggagca cttgatgaaa
      781 aaaagtttaa aaaagcttaa aaatcttcaa aaaagtgttg acaagtgaaa gtggctgtga
      841 tatactaata tagttgtcgc ttgagagaag caagtgacaa agacctttga aaactgaaca
      901 agacgaacca atgtgcaggg cgctacaacg taagttgtag tactgaacaa tg
//
LOCUS       DAVKLX010000110          898 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKLX010000110 DAVKLX010000000
VERSION     DAVKLX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.110"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            94..>898
                     /locus_tag="VW325_002102"
     CDS             94..>898
                     /locus_tag="VW325_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEU9735409.1"
                     /translation="MKSKLGITLRKVRKGKQISLCSVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLVLHNDDYTSSESFANLVQYIRKQYSSQSINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSNIIPSDEELLHLTDYLFKVEKWGYYEIILLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSLNKTNKQIVSQLAINCFILSIDKEEFSNCSYLISKIK
                     TLLDNELNFYEQTVFLYATGYFEFKRCQSTSGIEKMKQAIQVFDILGEDK"
BASE COUNT          317 a          145 c          129 g          307 t
ORIGIN      
        1 gaaattttct tcatacttgg acttcctttc cgtttttatt tattgtacaa taaataaaaa
       61 cggatttaat ttttgttggg aaaatgggaa attatgaaat caaaacttgg catcacactt
      121 agaaaagttc gtaaaggaaa acaaattagt ctatgctctg tagcagatga acatctttca
      181 aaatcacaaa tatctcgttt tgaacgtggg gagtcagaga tatcttgtat tcgactaatc
      241 aatattttag acaaattaca tattactttg gatgagttcc ttgttttaca caatgacgac
      301 tataccagtt ccgaatcatt tgctaatcta gtacagtaca ttcgtaagca atattcatca
      361 caaagtatca ataatatagc atgtctactt tctgatactt ctgattacac tctaaattct
      421 tttgagaaaa caatggtaaa atctatttta cacacaatgg attcgaacat cattccatca
      481 gatgaagagc ttcttcatct gacagactat cttttcaaag ttgaaaaatg gggctattat
      541 gaaatcattt tattaggtaa ctgtgtacgc acaattaact acaactccta cttcttatta
      601 actaaagaga tgttaaataa ctatatttac tcatctctga ataaaactaa taagcagatt
      661 gtctctcagc ttgctattaa ttgcttcatt ctaagcattg ataaggaaga attttctaat
      721 tgctcctatt taattagtaa gataaagaca ttgttggaca atgaactaaa cttttatgaa
      781 caaacagttt ttctctatgc aactggttac tttgaattta aacgttgcca atcaaccagt
      841 ggaattgaaa aaatgaaaca agccatacaa gtcttcgata ttttaggaga agataagt
//
LOCUS       DAVKLX010000111          788 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKLX010000111 DAVKLX010000000
VERSION     DAVKLX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.111"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(43..189)
                     /locus_tag="VW325_002103"
     CDS             complement(43..189)
                     /locus_tag="VW325_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735410.1"
                     /translation="MKTKEQTRKLAAGCSKHCFEVVDRTDEVSNHTHGKATLTWFEEI
                     FKEY"
     gene            347..565
                     /locus_tag="VW325_002104"
     CDS             347..565
                     /locus_tag="VW325_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU9735411.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT          251 a          116 c          146 g          275 t
ORIGIN      
        1 cgaaatctag tggaatgaat ctggaatagt ccatcgagct ttctaatact ctttgaaaat
       61 ctcttcaaac cacgtcaacg tcgccttgcc gtgcgtatgg ttactgactt cgtcagttct
      121 atccacaacc tcaaaacagt gttttgagca acctgcggct agtttcctag tttgctcttt
      181 ggttttcatt gagtataaca cattgttaga agttggttta aatttcctaa tcagtttgtt
      241 cacatttacc ttcgatatat tatatcccat agttaaggtt ggtcatacag atgattatag
      301 ccatggagcc gtaaaactta gtgtttcttt agttgacaaa gatgccatga aaaaaatatc
      361 tgtaactgta ataggatatt ttgaaataaa tatagatgaa aatatcaccg atattctata
      421 cgtaaatggt actgctattc tttatcctta tttacgttct attgtttcaa tagtttcggc
      481 aattgatagc agtgaagcaa tgttgctacc taccattaat gttttagagt tactagataa
      541 atctcaacct tttgaagaag aataatttat tagctcacta aattgagggt aaggaaaagt
      601 aaaagcagta agaaaaatgt cttgcattat acagcaactt tttgggaatg agtagatgga
      661 ttgaataaaa tttgattaag agtggatgat ttatctgtag attattattg gacagttagt
      721 cttgaagtag tctaagaatt aggttataat cagtagaagc cttgctaata atgaggaggt
      781 tagtttat
//
LOCUS       DAVKLX010000112          762 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKLX010000112 DAVKLX010000000
VERSION     DAVKLX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.112"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            230..>762
                     /locus_tag="VW325_002105"
     CDS             230..>762
                     /locus_tag="VW325_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEU9735412.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HTEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGH"
BASE COUNT          272 a          128 c          163 g          199 t
ORIGIN      
        1 accttagtca agaattagat gtttcactat gtttgagtaa gttgatgatt tcatttgata
       61 acaggtttga aactgttagg ctaggtggcc aaggctaatc atagccttgg tttagctgaa
      121 aaaacaggtt caagggttcc tgttgtcaaa tgaaatgtga tttaaggtat aagaaaacac
      181 ctctgtgcta tacttgttgt tcaccacaaa cacaaggaaa ggcacagaga tgcaagaaca
      241 ttatacccca aaagggaaac atttgacaat agataaccgt cgcttgattg agcggtggaa
      301 gaatgaaaat aagtccaatc gtgaaattgc aggcttgtta ggaaaggcgc ctcaaacgat
      361 tcatactgaa gtcaaaagag gtacaacttt acaacaagtg agaaaagggc tatacaaaaa
      421 ggtctattct gccgattacg cacaaactgt ttaccaattc aatcgaaaac ggtcggtgaa
      481 aaagttaatt ttaacaaagg aaatcagaga gaagatctta cactatcata agcaaaaatt
      541 ttcgcctgaa atgatggtta acaagaagca agtgaaagtt ggtatttcaa ccatctacta
      601 ctggtttcat aatggtcatt taggattgac gaaggccgac atgctttatc ccagaaaaag
      661 gaaaggtgtc aagaagcaag ctagtccgaa ctttaagccg gcaggtaaat ctatcgaaga
      721 acgtcctgac gttattaatc ttcgcttgga aaatggtcat ta
//
LOCUS       DAVKLX010000113          757 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKLX010000113 DAVKLX010000000
VERSION     DAVKLX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.113"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(5..>757)
                     /locus_tag="VW325_002106"
     CDS             complement(5..>757)
                     /locus_tag="VW325_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9735413.1"
                     /translation="KLILSNEDLGATNARNIGIKNSRGKYISFLDDDDEYMSDRILKL
                     MACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKK
                     EVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTI
                     NADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKVIKKAREEKI
                     FNEFLFLKYMLLYHFGFFYCIYDNYVQLKFRK"
BASE COUNT          253 a          142 c           73 g          289 t
ORIGIN      
        1 tttctcactt tctaaatttt aattgaacat agttgtcata tatacaatag aaaaaaccaa
       61 aatgatataa taacatatat ttcaaaaaaa gaaattcgtt aaaaattttt tcttctcttg
      121 ccttcttgat tacttttaaa gccttgcatt tgtctcctat taatagtaac cgctttatgt
      181 ttaaagaata atatttcttt gtaaccaata ttctctcgtt gaaactcaat aaattaaaat
      241 atttcctaca gtaattataa tattcttcat ctgcattaat tgttttttgt gtcactccag
      301 tgataccgtt ttctttactg tgagcgtagt aattcaccaa gaattctcgc actatatcaa
      361 tttggtatcc ttgagcaagt agttttaata aaacaacacc gtcctgatgt gaatctattt
      421 tctcaaaacc attaattaat tctagcacct cttttttaca caaccaaaat gacgtacctg
      481 ctatattgtg aaccatttga acaaacaagg gatttccaac aaaatcggtc ttctcctctt
      541 ctcgtgtacc atttggataa attattattc cataactata aactaaagct aaattcttca
      601 ttctactctt tttaaaacaa gccatcaact ttaaaattcg atctgacata tattcatcat
      661 catcgtctaa aaatgatata tacttacctc tagaattttt gatacctatg tttctggcat
      721 tagttgcacc taaatcttca ttacttaaaa ttaactt
//
LOCUS       DAVKLX010000114          569 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKLX010000114 DAVKLX010000000
VERSION     DAVKLX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.114"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..>569
                     /locus_tag="VW325_002107"
     CDS             <1..>569
                     /locus_tag="VW325_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9735414.1"
                     /translation="TKTNFINQKDKKGSIEDSVLNIVFVTTRTKYCSQILQLLLKYFD
                     KNPNKISEVYSILEANYGLVTEREYIDYTLAENTISELANLDLTKSYNQELIVTILKQ
                     FLKIEIERTEAHEEKFTFGRYKVPDSEKLKQYHRSILKLLANLYNIGSCETRFYIEKM
                     LYDYRRKILTYSESHRNTIFGDLRNIRKL"
BASE COUNT          223 a           63 c           97 g          186 t
ORIGIN      
        1 atacaaagac taattttatt aatcagaagg ataaaaaggg atctattgag gattcagttt
       61 taaatattgt atttgtaact acacggacaa aatattgtag tcaaattctc caattattgt
      121 taaagtactt tgataaaaat cctaataaaa tttctgaggt ttatagtatt cttgaagcaa
      181 actacgggtt ggttacagaa agagaatata ttgattatac cttagctgaa aataccattt
      241 ctgaacttgc taatcttgat ttgacgaaaa gttataacca agaattaatc gtgacaattt
      301 tgaagcagtt tcttaaaatt gaaatcgaaa gaacagaggc tcatgaagag aaatttactt
      361 ttgggcgcta taaagtcccg gatagtgaaa aattaaaaca gtatcataga agtatactaa
      421 aacttttagc gaatttgtat aatataggga gttgtgaaac tagattttat attgagaaaa
      481 tgctatatga ttacagaaga aaaattttga cctattcaga aagtcatcgt aatactatct
      541 ttggagattt gagaaatatt aggaaatta
//
LOCUS       DAVKLX010000115          568 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKLX010000115 DAVKLX010000000
VERSION     DAVKLX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.115"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..>568
                     /locus_tag="VW325_002108"
     CDS             <1..>568
                     /locus_tag="VW325_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9735415.1"
                     /translation="PIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDI
                     TSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKG
                     AEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAP
                     KGYTLEDLLATVKYYVEHPNERPHSDNGF"
BASE COUNT          183 a          119 c          126 g          140 t
ORIGIN      
        1 ccgattcgtc atccagaacg tttaggaaaa ccaaatgcgc aaattaccta cactgatgat
       61 gagattcaag tagccaagtt ggcaggcaag tacacaacag aagacggtta tatctttgat
      121 cctcgtgata taaccagtga tgagggggat gcctatgtaa ctccacatat gacccatagc
      181 cactggatta aaaaagatag tttgtctgaa gctgagagag cggcagccca ggcttatgct
      241 aaagagaaag gtttgacccc tccttcgaca gaccatcagg attcaggaaa tactgaggca
      301 aaaggagcag aagctatcta caaccgcgtg aaagcagcta agaaggtgcc acttgatcgt
      361 atgccttaca atcttcaata tactgtagaa gtcaaaaacg gtagtttaat catacctcat
      421 tatgaccatt accataacat caaatttgag tggtttgacg aaggccttta tgaggcacct
      481 aaggggtata ctcttgagga tcttttggcg actgtcaagt actatgtcga acatccaaac
      541 gaacgtccgc attcagataa tggttttg
//
LOCUS       DAVKLX010000116          499 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKLX010000116 DAVKLX010000000
VERSION     DAVKLX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.117"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..>499)
                     /locus_tag="VW325_002109"
     CDS             complement(<1..>499)
                     /locus_tag="VW325_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9735416.1"
                     /translation="LGHKALKIIGLNPSGRDGGNPKVTYPALGQFGKVIPGSTTNQDV
                     TISFYAKANKNGIMLRTRLGNIGYKTGNVTLSTEIKRYVVHIPKGWTNESKQTTNEWL
                     FNFNQEGTVWIWMPKFEISDVDTSYSEAPEDIEGQISTVESTFKQRANSLEAGVNRLT
                     EGLRT"
BASE COUNT          129 a          110 c           88 g          172 t
ORIGIN      
        1 tttgttctga gaccttcagt cagacgattc acaccagctt cgagcgagtt ggctcgttgt
       61 ttgaaggtcg attctactgt tgaaatctga ccttctatat cttcaggagc ttctgaataa
      121 gaagtatcta catcgcttat ttcaaacttc ggcatccaaa tccaaacggt tccttcctgg
      181 ttgaaattga acaaccattc atttgtggtc tgcttggatt cgtttgtcca accttttgga
      241 atatggacaa catatcgctt aatttctgtc gacaatgtca catttccagt tttatatccg
      301 atattcccta atcgagttct tagcattatt ccatttttat ttgccttagc ataaaaacta
      361 atggttacat cttgattagt cgtacttccg ggaattactt tcccgaattg acccagagct
      421 ggataagtaa ccttgggatt acctccatca cggccagatg gattcaaacc tataatttta
      481 agagccttgt gtccaagat
//
LOCUS       DAVKLX010000117          412 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKLX010000117 DAVKLX010000000
VERSION     DAVKLX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.118"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..89)
                     /locus_tag="VW325_002110"
     CDS             complement(<1..89)
                     /locus_tag="VW325_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9735417.1"
                     /translation="MWYNLFMAYSIDFRKKVLSYCERTGSITEA"
     gene            complement(179..252)
                     /locus_tag="VW325_002111"
     tRNA            complement(179..252)
                     /locus_tag="VW325_002111"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(216..218),aa:Asn,seq:gtt)
     gene            complement(258..373)
                     /gene="rrf"
                     /locus_tag="VW325_002112"
     rRNA            complement(258..373)
                     /gene="rrf"
                     /locus_tag="VW325_002112"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          129 a           94 c           65 g          124 t
ORIGIN      
        1 gcttctgtta tactacctgt tcgctcacaa taagagagaa cttttttacg aaaatctatt
       61 gaatatgcca taaaaagatt ataccacatt gtgtactatt tttggttcat tttactataa
      121 cacaaaatag attattatta cataacaaaa aagaggtcta aacctcttaa ctcaattact
      181 ccgccagtag gactcgaacc tacgacatca tgattaacag tcatgcgcta ctaccaactg
      241 agctatggcg gattaaagct aagcgacttc cctatctcac agggggcaac ccccaactac
      301 ttccggcgtt ctagggctta acttctgtgt tcggcatggg tacaggtgta tctcctaggc
      361 tatcgtcact taactctgag taatacctac tcaaaattga atatctattc aa
//
LOCUS       DAVKLX010000118          367 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKLX010000118 DAVKLX010000000
VERSION     DAVKLX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.119"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..>367
                     /locus_tag="VW325_002113"
     CDS             <1..>367
                     /locus_tag="VW325_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9735418.1"
                     /translation="SRTARGVAVPHGNHYHFIPYEQMSELEKRIARIIPLRYRSNHWV
                     PDSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVS
                     RYIPAKDLSAETAAGIDSKL"
BASE COUNT          115 a           91 c           72 g           89 t
ORIGIN      
        1 caagtcgaac cgccagaggt gtagctgtcc ctcatggtaa ccattaccac tttatccctt
       61 atgaacaaat gtctgaattg gaaaaacgaa ttgctcgtat tattcccctt cgttatcgtt
      121 caaaccattg ggtaccagat tcaagaccag aacaaccaag tccacaatcg actccggaac
      181 ctagtccaag tctgcaacct gcaccaaatc ctcaaccagc tccaagcaat ccaattgatg
      241 agaaattggt caaagaagct gttcgaaaag taggcgatgg ttatgtcttt gaggagaatg
      301 gagtttctcg ttatatccca gccaaggatc tttcagcaga aacagcagca ggcattgata
      361 gcaaact
//
LOCUS       DAVKLX010000119          366 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065262
            SAMN33606440-rid17950823.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKLX010000119 DAVKLX010000000
VERSION     DAVKLX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606440
            Sequence Read Archive: SRR23727360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722402.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722402.1
            Genome Coverage       :: 358x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:31:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,114
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 64 of 130
            Pseudo Genes (incomplete)         :: 57 of 130
            Pseudo Genes (internal stop)      :: 35 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606440-rid17950823.denovo.120"
                     /strain="AUSMDU00065262"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..>366
                     /locus_tag="VW325_002114"
     CDS             <1..>366
                     /locus_tag="VW325_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9735419.1"
                     /translation="LLVLTDRRSRHQIIRLIPNKTAESVNQALTLLLGEHRILSITAD
                     NGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRRWLPKGTKKTTPKEVAFIE
                     NWINNYPKKCLDYKSPSEFL"
BASE COUNT          121 a           71 c           74 g          100 t
ORIGIN      
        1 ctgttagtct taaccgaccg gcggagcaga caccaaatta taaggttaat tccaaataaa
       61 actgctgaat ctgtcaatca ggcgcttacg ttactattag gggagcatcg tattctgtcc
      121 attactgcag ataatggttc ggagttcaaa cgattgtctg aggtatttcc tgaggaacat
      181 atctactacg cacatgctta ctcttcatgg gagagaggtt caaatgaaaa tcataatcga
      241 ttaattcgga gatggttacc taaaggaacc aagaaaacga ctccgaaaga agtagctttt
      301 atcgaaaatt ggattaacaa ctaccctaaa aaatgcttgg actacaagtc gccaagtgaa
      361 tttctt
//
